LOCUS       DAYYKN010000001        61089 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000001 DAYYKN010000000
VERSION     DAYYKN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.001"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..220
                     /locus_tag="ACHBB7_000001"
     CDS             <1..220
                     /locus_tag="ACHBB7_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9207991.1"
                     /translation="EKNELLLNTKFQLINSDGSSSDMLFYEFDGENYHCFNRKDNSIS
                     MYADIKREVKKILKVEEQYYKEIEKRNQ"
     gene            389..1714
                     /locus_tag="ACHBB7_000002"
     CDS             389..1714
                     /locus_tag="ACHBB7_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9207992.1"
                     /translation="MENIEETFLKVLSPYISDSILRFQEQEKNYKFGFDIKNYNDSLH
                     VVFQYNKTGKIGIKEVHIFQIFLAKVNIEASKSKLNYTTLLDTNALFFSKKLLPYLHS
                     YEWALRKLIYLLAPAYFSDNWAEESIPNEIISSIKRKQIQAIGKFDSKNLLQWIDLYD
                     FEEYLFGENYILVTQENEENIVRYKEISQDQLLKLFQDNSHTISDSYSLWSEVFSKYM
                     DVKLEEIQTDMSLIREGRNIVSHNKEIEMALYQKLIKKLKRYTNQLEKAFQKILAGDI
                     AGEDLSDMSDDFEGYIGNYFSNIDLAGVTKLSESLSAMKVLNTDSMKKSMESIGQTLS
                     VQQEIMQNFDTEGIRRATEGINQSLQSIKILNTDSMKRSIESIGQTLSALSAQRAIMQ
                     NFDTKGIRSATEGINQSLQSMKIVNTDSMKRSMESIGKISSTNQVLKSE"
     gene            1814..2632
                     /locus_tag="ACHBB7_000003"
     CDS             1814..2632
                     /locus_tag="ACHBB7_000003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9207993.1"
                     /translation="MVQGLFGGGTSYEGQSISDIKEDVNNWKIYTENVKSQIVEKKEA
                     LIKNGFWNKIPWNFQQTINESIFCLGTFLSDFQIVESAFEENFISEREIKLLRKIGKN
                     SVQFNHSYGKYFKEEYEWREYGNPEFEIVESIYQHGRDFFVTLQDASNAAGRLEDYMS
                     NNSIYNKVNIFGNSYNTNIQQGHNNNMIVSIDDMSELLSKLDLLLENLNDSLPEEEVE
                     EAQEYIETIKDEAIKEKPKKTMLRFAVDSLKKLKWSTEFLASVATIAQLVAPLL"
     gene            complement(3071..4216)
                     /locus_tag="ACHBB7_000004"
     CDS             complement(3071..4216)
                     /locus_tag="ACHBB7_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9207994.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(4277..4924)
                     /locus_tag="ACHBB7_000005"
     CDS             complement(4277..4924)
                     /locus_tag="ACHBB7_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9207995.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            5118..5411
                     /locus_tag="ACHBB7_000006"
     CDS             5118..5411
                     /locus_tag="ACHBB7_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9207996.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            5455..5766
                     /locus_tag="ACHBB7_000007"
     CDS             5455..5766
                     /locus_tag="ACHBB7_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9207997.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            5763..6387
                     /locus_tag="ACHBB7_000008"
                     /pseudo
     CDS             5763..6387
                     /locus_tag="ACHBB7_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            6528..6686
                     /locus_tag="ACHBB7_000009"
     CDS             6528..6686
                     /locus_tag="ACHBB7_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9207998.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            6687..7049
                     /locus_tag="ACHBB7_000010"
     CDS             6687..7049
                     /locus_tag="ACHBB7_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9207999.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            7086..7934
                     /locus_tag="ACHBB7_000011"
     CDS             7086..7934
                     /locus_tag="ACHBB7_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM9208000.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            7924..9399
                     /locus_tag="ACHBB7_000012"
     CDS             7924..9399
                     /locus_tag="ACHBB7_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM9208001.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            9665..10072
                     /locus_tag="ACHBB7_000013"
     CDS             9665..10072
                     /locus_tag="ACHBB7_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM9208002.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            10075..10290
                     /locus_tag="ACHBB7_000014"
     CDS             10075..10290
                     /locus_tag="ACHBB7_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208003.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            10294..10674
                     /locus_tag="ACHBB7_000015"
     CDS             10294..10674
                     /locus_tag="ACHBB7_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9208004.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            10808..10963
                     /locus_tag="ACHBB7_000016"
     CDS             10808..10963
                     /locus_tag="ACHBB7_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM9208005.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            11032..11505
                     /locus_tag="ACHBB7_000017"
     CDS             11032..11505
                     /locus_tag="ACHBB7_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM9208006.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            11502..13196
                     /locus_tag="ACHBB7_000018"
     CDS             11502..13196
                     /locus_tag="ACHBB7_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9208007.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            13162..13347
                     /locus_tag="ACHBB7_000019"
     CDS             13162..13347
                     /locus_tag="ACHBB7_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208008.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            13351..14526
                     /locus_tag="ACHBB7_000020"
     CDS             13351..14526
                     /locus_tag="ACHBB7_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9208009.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            14519..16042
                     /locus_tag="ACHBB7_000021"
     CDS             14519..16042
                     /locus_tag="ACHBB7_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9208010.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            16098..16382
                     /locus_tag="ACHBB7_000022"
     CDS             16098..16382
                     /locus_tag="ACHBB7_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9208011.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            16369..16704
                     /locus_tag="ACHBB7_000023"
     CDS             16369..16704
                     /locus_tag="ACHBB7_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9208012.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            16999..17625
                     /locus_tag="ACHBB7_000024"
     CDS             16999..17625
                     /locus_tag="ACHBB7_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208013.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            18367..19203
                     /locus_tag="ACHBB7_000025"
     CDS             18367..19203
                     /locus_tag="ACHBB7_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM9208014.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            19267..19698
                     /locus_tag="ACHBB7_000026"
     CDS             19267..19698
                     /locus_tag="ACHBB7_000026"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9208015.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(19760..21082)
                     /locus_tag="ACHBB7_000027"
     CDS             complement(19760..21082)
                     /locus_tag="ACHBB7_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM9208016.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(21236..22876)
                     /locus_tag="ACHBB7_000028"
     CDS             complement(21236..22876)
                     /locus_tag="ACHBB7_000028"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM9208017.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            23209..23679
                     /locus_tag="ACHBB7_000029"
     CDS             23209..23679
                     /locus_tag="ACHBB7_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM9208018.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            23901..25004
                     /locus_tag="ACHBB7_000030"
     CDS             23901..25004
                     /locus_tag="ACHBB7_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM9208019.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            25288..25968
                     /locus_tag="ACHBB7_000031"
     CDS             25288..25968
                     /locus_tag="ACHBB7_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9208020.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            26058..27092
                     /locus_tag="ACHBB7_000032"
     CDS             26058..27092
                     /locus_tag="ACHBB7_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM9208021.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            27106..27441
                     /locus_tag="ACHBB7_000033"
     CDS             27106..27441
                     /locus_tag="ACHBB7_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208022.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            27425..29905
                     /locus_tag="ACHBB7_000034"
     CDS             27425..29905
                     /locus_tag="ACHBB7_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208023.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            29961..31520
                     /locus_tag="ACHBB7_000035"
     CDS             29961..31520
                     /locus_tag="ACHBB7_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9208024.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            31517..32881
                     /locus_tag="ACHBB7_000036"
     CDS             31517..32881
                     /locus_tag="ACHBB7_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9208025.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            33052..34122
                     /locus_tag="ACHBB7_000037"
     CDS             33052..34122
                     /locus_tag="ACHBB7_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9208026.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            34137..34805
                     /locus_tag="ACHBB7_000038"
     CDS             34137..34805
                     /locus_tag="ACHBB7_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9208027.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            34825..35403
                     /locus_tag="ACHBB7_000039"
     CDS             34825..35403
                     /locus_tag="ACHBB7_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9208028.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            35804..36715
                     /locus_tag="ACHBB7_000040"
     CDS             35804..36715
                     /locus_tag="ACHBB7_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9208029.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(36803..37720)
                     /gene="rnhC"
                     /locus_tag="ACHBB7_000041"
     CDS             complement(36803..37720)
                     /gene="rnhC"
                     /locus_tag="ACHBB7_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM9208030.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            37922..38359
                     /gene="zapA"
                     /locus_tag="ACHBB7_000042"
     CDS             37922..38359
                     /gene="zapA"
                     /locus_tag="ACHBB7_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM9208031.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            38418..38966
                     /locus_tag="ACHBB7_000043"
     CDS             38418..38966
                     /locus_tag="ACHBB7_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM9208032.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            39081..41441
                     /locus_tag="ACHBB7_000044"
     CDS             39081..41441
                     /locus_tag="ACHBB7_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM9208033.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            41528..41842
                     /gene="trxA"
                     /locus_tag="ACHBB7_000045"
     CDS             41528..41842
                     /gene="trxA"
                     /locus_tag="ACHBB7_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM9208034.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            42024..43802
                     /gene="uvrC"
                     /locus_tag="ACHBB7_000046"
     CDS             42024..43802
                     /gene="uvrC"
                     /locus_tag="ACHBB7_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM9208035.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            43947..44846
                     /locus_tag="ACHBB7_000047"
     CDS             43947..44846
                     /locus_tag="ACHBB7_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9208036.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            44839..46101
                     /locus_tag="ACHBB7_000048"
     CDS             44839..46101
                     /locus_tag="ACHBB7_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9208037.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            46262..48160
                     /locus_tag="ACHBB7_000049"
     CDS             46262..48160
                     /locus_tag="ACHBB7_000049"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM9208038.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(48490..49209)
                     /locus_tag="ACHBB7_000050"
     CDS             complement(48490..49209)
                     /locus_tag="ACHBB7_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM9208039.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    49399..49446
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            49546..49878
                     /locus_tag="ACHBB7_000051"
     CDS             49546..49878
                     /locus_tag="ACHBB7_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208040.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            49865..51868
                     /locus_tag="ACHBB7_000052"
     CDS             49865..51868
                     /locus_tag="ACHBB7_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM9208041.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            51887..52357
                     /locus_tag="ACHBB7_000053"
     CDS             51887..52357
                     /locus_tag="ACHBB7_000053"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM9208042.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            52378..52962
                     /locus_tag="ACHBB7_000054"
     CDS             52378..52962
                     /locus_tag="ACHBB7_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM9208043.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            52977..53963
                     /locus_tag="ACHBB7_000055"
     CDS             52977..53963
                     /locus_tag="ACHBB7_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM9208044.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            53953..54264
                     /locus_tag="ACHBB7_000056"
     CDS             53953..54264
                     /locus_tag="ACHBB7_000056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM9208045.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            54286..56067
                     /locus_tag="ACHBB7_000057"
     CDS             54286..56067
                     /locus_tag="ACHBB7_000057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM9208046.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            56060..57436
                     /locus_tag="ACHBB7_000058"
     CDS             56060..57436
                     /locus_tag="ACHBB7_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM9208047.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            57443..58078
                     /locus_tag="ACHBB7_000059"
     CDS             57443..58078
                     /locus_tag="ACHBB7_000059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM9208048.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            58108..59463
                     /locus_tag="ACHBB7_000060"
     CDS             58108..59463
                     /locus_tag="ACHBB7_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9208049.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(59655..61049)
                     /locus_tag="ACHBB7_000061"
     CDS             complement(59655..61049)
                     /locus_tag="ACHBB7_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM9208050.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT        20502 a        10217 c        12761 g        17609 t
ORIGIN      
        1 cgagaaaaat gaattactac tcaataccaa atttcagtta attaattcag atggaagctc
       61 aagtgatatg cttttttatg agtttgatgg tgagaattat cactgcttca accgaaagga
      121 caacagtata tctatgtatg ctgacataaa aagagaagtt aaaaaaatat taaaagtaga
      181 agaacaatac tataaagaaa tagaaaaaag aaaccagtaa ttaacacagt tatcaaggta
      241 aagaaaaagt tctctttctg tttgctagtt gtggcttatg tagcaaatat caagttaata
      301 ggaaaattaa aattgttgaa aggaaagaag ttgatgacat attagtaagc aagttgttga
      361 atatttatat tatcctaaaa aaggaagaat ggaaaatatc gaagaaacct ttctgaaagt
      421 gttgtcacca tatattagtg attctatctt aagatttcag gagcaggaaa aaaattataa
      481 atttggcttt gacataaaga actataatga ttcgttacac gtagtatttc aatataataa
      541 gactggtaag ataggaataa aagaggtaca tatatttcaa attttcctag ctaaggtcaa
      601 tattgaagca agtaaatcaa aattaaacta cactaccctt ttagatacaa atgcgttgtt
      661 tttttcgaaa aaactattac cgtatttgca tagttacgaa tgggcattaa gaaaattaat
      721 ctatttatta gctccggcat atttttctga taactgggca gaggaatcaa ttccaaatga
      781 aatcatttcg tcaataaaaa gaaaacaaat acaagcaata ggaaagtttg actcaaagaa
      841 tcttttacaa tggatagatt tatatgattt tgaagaatat ctctttggag aaaattatat
      901 tttagttaca caagaaaatg aagagaatat tgtaaggtat aaagaaatat ctcaggatca
      961 attattgaag ttatttcaag ataacagtca cactatatct gattcatatt ctttgtggag
     1021 tgaagtattt agtaaatata tggatgttaa gctagaagaa attcaaacag acatgagttt
     1081 aatcagagaa ggtaggaata ttgtaagcca taacaaagaa atagaaatgg ctttatacca
     1141 aaaattaata aagaaattga agagatatac aaatcaacta gagaaagctt ttcaaaaaat
     1201 attggctgga gatattgctg gtgaggacct tagtgatatg tcagatgatt ttgaaggata
     1261 tattggaaat tattttagta atatcgattt agcaggtgtt actaaattgt cagagtctct
     1321 atcagcaatg aaagtattaa atacagattc catgaaaaag tcgatggagt caataggtca
     1381 aacattatca gtccagcaag aaattatgca gaactttgat actgaaggta tcagaagggc
     1441 aacagaggga ataaatcaat ctctacaatc aataaaaata ttaaatacag attccatgaa
     1501 gaggtcgata gagtcaatag gtcaaacatt atcagcttta tcagctcagc gagcaatcat
     1561 gcaaaacttt gatactaaag gtatcagaag tgcaacagag ggaataaatc aatctctaca
     1621 atcaatgaaa atagtaaata cagattccat gaaacgatcg atggaatcaa ttggtaaaat
     1681 ttcatcaaca aaccaagtat tgaagtcaga gtaaataaaa caggaacgat ggcaatcatg
     1741 ttaccacacg aaatgctatc atttgtagca agtattggat ttagagtaat tattatagag
     1801 aggatgagat aaaatggttc aaggactttt tggtggaggg acttcttatg aaggtcaatc
     1861 gatttcggac attaaggaag acgtcaataa ttggaagata tatacagaaa atgtaaaatc
     1921 acagattgtt gaaaaaaaag aggctttaat taagaatggt ttttggaaca aaataccatg
     1981 gaattttcaa caaactatta atgaatcaat cttctgttta ggaacatttc ttagtgattt
     2041 tcagatagtt gaatcagctt ttgaggaaaa ttttatttct gagagagaga taaaacttct
     2101 aaggaaaatt ggtaaaaatt ctgtgcaatt taaccatagt tacggaaaat actttaagga
     2161 agaatacgaa tggcgcgaat atggaaatcc agaatttgaa atcgttgaat caatttacca
     2221 acatgggaga gatttttttg tgacgttaca agatgcaagt aatgcagcag gacgactgga
     2281 ggactatatg agcaacaatt caatatataa taaagtaaat atatttggaa atagttacaa
     2341 cactaatata cagcaaggcc acaataataa tatgattgtt tctattgatg atatgtcgga
     2401 attattatca aagcttgatt tgcttttgga gaatttgaat gattccttac ctgaagaaga
     2461 ggtagaagag gctcaggagt atatcgaaac cattaaagat gaagctatta aagaaaagcc
     2521 taaaaaaact atgctgaggt ttgcggtaga tagtctaaaa aaacttaaat ggagtacaga
     2581 attcttagct tctgttgcaa caatagctca acttgttgct ccactgctat aagtggagaa
     2641 tgaggtgttc ttcaattaac cgacttgcca agttgtggta atggtttgag ataatgaaag
     2701 ctttgtattc tatagagatt gatgtatctg aatgaatgct tgtttttttg gatatagctg
     2761 attaatcagt ggtgttacat tatttataag tcgtttgatt aactagttct tacgatttct
     2821 tatcaaaagc acgccacgca acttgaatac gtttttgaat acgactaaaa aaccacttaa
     2881 ggtgaattag acttaataaa gtgattagtt ctattcaaaa atcgttgaaa taccaactat
     2941 cgtcaattat ctttaatgag cttaacttca tggcggaaga agaagaatag actataaagc
     3001 taggagaaac cttgatatga tagggtttct ccttttttta ttttttactg tatgccatat
     3061 tgtatgccat ctagtcaata tttaagtgtt gtactaattt ttcaacagtc ttttctttcg
     3121 tttcttttgc caagtgaccg taacaatcga tcgtctgact aatttttgta tgccgtaatc
     3181 tagtttgaac ttctaccata gtagctccag cgtccaagag cattgaagca tgagtgtgtc
     3241 ttgctacatg cacaccgaga aatggaacca tagctttttc acaaatagta atcaatcttg
     3301 cataaatagc gtggtgtgtg attagttctt tatagatacc acaaaaaatc atagtcggat
     3361 cagttacacc aagtgtcagc atatatttcc gctgattaat ctgccatttt tttaatatag
     3421 aaattgttct ttcatctagt ggaagaaccc cagcactaca atcagtttta ggatcactga
     3481 ttttatagcc atttttggat tggcttagtg ttttattgat ggtgaccgta ttttcttgaa
     3541 aatcaatgtc tgaccaattc aatgcaaggg cttcactgat cctaaccccg ctataaaaga
     3601 gaaagcgtag taacgtttta tcaaagtcgt ttcgataagt ttcaggtttg ctgtttaggt
     3661 aatcaaacaa ggtgatcagt tgttctttac tgtaaacttt gaccttttct ttatgcttag
     3721 tggtggttct tttaggcatt aatacgttgt ctagtggatt agtctcaata atcccaagat
     3781 tgatagcgta gctaaaaata cgcttagaag ttgaatgaag taccttgtat tggtcagtag
     3841 tttcagccca gctatttaca atttgttggg ctttttttac cgtgacttca ttcacacgaa
     3901 gatttccata ttttggaaga atccactttc taaacttgat ttcagttttc atgaaggtgg
     3961 cttctcggac agtggtttta tatgtttcta gccacaagtc ataaacttct ttaaacgttt
     4021 cgttgttcgc ttttttaata ccgtttttat cgtagtccac ttgcaagcgt gacaaggcaa
     4081 gctgggcttc tttttttgtt ttgtaacctc gtctacgaac cttatcaggt ttgccagtca
     4141 ttttgttcat tccaagatag gcttcataga gccaaaattt acctttttta gtttcatatt
     4201 gcttgaatgt tgccataata gttttccctc catagtgtag caagccgtgg gggcggttgg
     4261 aggaggcagt ttattattat tttgaatagt gtttatttag ctcagtaaca agagctgtga
     4321 actcttttag aagatcattt tcataaaatt cttgatactc ttttttctca tctgtatttt
     4381 tttctgaatc attaattaat gagtcatatc gtaaaagcgt atttagaaga gctgcgagat
     4441 aagcaattgg ggtactcccg tcaggaaatt caattcgaga tcctgtttta agatatttta
     4501 ttgcgtttgt taaataaatc ttctgtgaga cagtcaggtt atcttcttcg atgttattga
     4561 tagcattaga aagattagaa tcaattatag agttattttt ttcaatagtt tttttcaatt
     4621 gcttttcaag cacatttcgc ttaatttcgg gcatcatatg catatctctt aatgtataag
     4681 atccgtttaa tagataggta gtggatacat ctaatacctt tgaaagttgt ttaagcctgg
     4741 tgttattggg aagattatag ccattttccc aattactaac ggcaccttta ctggccggtg
     4801 gggataattt acctccaagt tgttccattg agagtccctt ttcaattcta gccagtctaa
     4861 ttctttgtcc tgtctccact gcttcggggt atttttttct agccacattt tcaccaccat
     4921 tcatatattt tctctactat atcacgattt aaatatgtgt gaaacataaa agttcaaaaa
     4981 agtatgttaa aattcttgca aatgaaaagg tatgatggta tattgagttt ataagaggta
     5041 tagaaatgta ttcaagagcc tcttgcattt ttttaaaatc tttgtataga aatgtatgct
     5101 aacgaggggg gataaatatg gaattaaaaa ttgacaaact cacgcttgat gatccagctt
     5161 ttaaaattat tgacgatcga gttgttcaac aagtagagca aactcaagga acgttacttt
     5221 cattattaga aaaaaagtac cagtttccaa aatatatgaa caaggcacaa gctgctaagt
     5281 atatgaacgt ttcatacaat actatgatgc agaaatatgt tcctaacggt ttaaagttga
     5341 ttatagttga tggagtcgta agaatttcac aagatgaatg tgatcgcttt atggaagaat
     5401 accaaaaata aacgcaagcc gtgggggcaa agggattaat aggaggaaat aataatggaa
     5461 tcgacacaaa ataaagcaat cgaaaaagta ttatcaacaa ttcttacaga agacacagcg
     5521 gcagaattag caaacttgga aggcaaagcg ctagaagaaa cgtttgaatg tttgtacgaa
     5581 caaatggact atcaaaaatt gctaccacaa gaaccaacgg taagcggagt attgcgtggc
     5641 ttgaacgatt tagtacaagc agagtttaaa gaacgacttt ctattgaaga gtatcaagaa
     5701 attttgtatc agcaagttga gttgttagcg agcttgctag ggcttgaact ggaggacgtt
     5761 gaatgaaaac tgaaatttgg aacggacaca ttataagatt tgtcgatatt aatgatgaat
     5821 ggtgggcagt ggcgaaagat gttgcggaag cgttaggtct taaacaggtt actagagcca
     5881 ttcactcttt gcctaaagat ggggttacta ctagtaaggt catcgattca ttgggcagaa
     5941 cacaagatgt aaacatcatc aacgaaaaaa aatatttacc gcatggcatt caaaagccgt
     6001 aaaaaagaag ccgaggcatt tcaagactgg attttcgaca tcatcaaaga actacgccag
     6061 tccacaggac ttgaaggctt tcaagtattc cgcatgctag acaaagaaca ccaaaaagaa
     6121 gcaatgacta aattaagcca cgctattact gaaccaaaac cagtagacta cattaaagcg
     6181 aatgtgatag ccaataaagc tgtatcaact atttatggtc attccaaaat ggtaaaaaag
     6241 aaagatatga ctcctgaaat gttggttgat cgtgaaccta ttttagatga gacagtagaa
     6301 ttaatgactg ttaaagaaaa atatgggttg cagttttcag tgagtgagaa aatttataat
     6361 cgttctgctg aattgcagac aacttaggag cattaacatg atcgagaaca aagaaaaagc
     6421 cttatccata acagtcttgg cggacaggat aaaggcaact taattgggat aaaacccgat
     6481 attctttgtt ccgattatac catattaaaa aatcggaggg aaccattatg aaacgaaact
     6541 taattatagg aagcatttta atcggcggaa ttttcggctt attaccatta attgcaaaaa
     6601 tagctatctt tgcaggatta gcgttatatc tcttcactgc ttatgacgag tacgactatc
     6661 aattaagaac tggagggcat aagtagatgt acaaaacaaa actattaaac caactcgata
     6721 gccttgaatt agaggaaatt aatcaaggga tcgctgaact agaaaataat atcgggaaaa
     6781 cctactttgg aaattcattc aatgaaaaac taacagtttt gtatgtactc aaaaaacatg
     6841 cagaacacaa aataatttgt cgagaaatca acgagctaaa aaatcagatt ttaaccgcat
     6901 ggttaaacat tactgatatg caagaagcaa gggtgaaaac atttaataca tgggtaaagt
     6961 atcagaatca attaaaaggt gctgaatttg ttcgggatgg gttgaaatac gaacttgagc
     7021 agttgaagct catggaggtg tctgaatgat ttataccagt attttgaaaa aggaggagag
     7081 gtttattggc aaataattat caagcagcaa cggaattaca aaagtcggga tggtcaattt
     7141 atcccttatc tcctggaact aaaattccaa taaaaggctc tagtggttgg aatgatgcaa
     7201 ctaacgattc taatcaaatt aatgaatggt ggacggaaga tgcaactaga aatattgggt
     7261 taatgcttga agatcatcaa atggtggtag ttgacctaga tcaacattct gaaacacgaa
     7321 acggcgttga gaattataaa aaacttgctt caatgtatga tccatttcct tctacttata
     7381 cggaaatcac accaggtaaa ggaatccatt tcttctttaa aattcctgat ggtgtaatta
     7441 tccaacaaga aacaggtgca tttgctgata tcttcgggcg tgatgataaa ggtaaaagtt
     7501 taagcgggat tgatattatc actaaaggag ttccaatagc gccaactatg attatttcaa
     7561 gaagtgttgg gagagcatat gaacctattg aaaacgtatc tctaaataat attgcaccag
     7621 ctcccgattg gctagtgaac ctactgctaa aaaaaccgca agaaataaag actcaatacc
     7681 gacccgtagc aaaaacaggg acagcaaaaa aattagatat gattgttcaa ggcgctggcg
     7741 aaggtagtag aaacgatcat ttaactaagt tatgcggttg gttactatgg cacggtgtag
     7801 ataatcaaac tttagcagaa ttgatttata cagcaaatgc gtacaatgaa ccaccgctaa
     7861 aagataaaga agtgaatcaa attattcgat cgatgattaa aaaagatttg agggggaaag
     7921 ctcatggctc ataagcataa attagatagt attttagact ttcccgaagc cagcgagcgt
     7981 gaagacaata tcattgaatt gaaaacctgg atgtcacgat taagatgcaa taaagacgat
     8041 cagattaaaa gtaacagtgt tgtgaatgca gaattgattt taaccaatga tagcaattta
     8101 gctggaacaa ttgcttataa cgaatttagc ggatacattc acctattaaa agattctcct
     8161 tggatcaatc gtaccgctgg agaatgggaa gacagttttg aggatgcctt aacggcatat
     8221 attgaagaaa actacaatgt agtttttgat gacaacaaaa tacacaaagc agtagtaaat
     8281 gtggctagaa agaatgtttt caacccagta aaagaacgta tcgaaaaagt taaatgggat
     8341 caacagccta gactggaaac actgtttatt gaccttttag gggtagacga caacctttac
     8401 acacgagaag taacaaaacg ttggatagtc ggatcagtcg cacgtatcta taaaccagga
     8461 ataaagtttg agatcgttcc agttttggat ggaccgcaag ggattggtaa gagtaccgtt
     8521 ccagcattac tttacacgga tgattttttc accgattcat tagattcgct aggtgaaaag
     8581 aaagatgatt atatgcagct tcaaggcaat gtaattattg aacttggaga attatcttca
     8641 atgaacaaaa ctaaaattga acagataaaa aacttcatca gtgccaagat cgataaaatt
     8701 agacctcctt atggtcggaa tgtcatagct tgggcaaggc aatgtgtatt tattggaacg
     8761 tcgaatgatg ggcagtattt gaaagatgat acaggaaacc gccgttttta tcctttgcct
     8821 tgtaagaata agccaaagat gaatccgttt aaaacaaagg atgaatattt tcttcaagtg
     8881 ttagctgaag caaaggtgct ttttgataaa ggacaacgaa tctattttag tcccgatgaa
     8941 gataaagaag tcttagaaat tgcaaaagac tatcaagagg atgccaagat ggagaatcca
     9001 attaaagaag caatcactaa atatttggaa atggaaatcc cgtataaatg ggaagaagct
     9061 ccagcgtggg cgagacgttc ttattatcag aactatcctg acagtaaatg taatgagaaa
     9121 aatttacagc atttctataa tggagctact atgcaacaag agttttattt agttgatagc
     9181 gtactcaccg ctgatatttt ggaagctgtt tttgataaac aagcaaagga tttattgaat
     9241 ggtcgatcag atgcagaaac taaaaagatt gccttgatta ttagcagtat tcctggttgg
     9301 gaacggaagc aactgccaaa aagaaataaa cgacgaggat tttacaataa gtcaaatgcc
     9361 tatgaaaata aaaatcgagc tggaaagtac aaaaagtaac gggtagtaac gggtcagtaa
     9421 cacctagccc gttactagtt tacccttact gccccaaggg atacagcaat ttggtaacgg
     9481 ggtaacacct tatttcctta aaacttttga aatagttagc taatatgctg gggaataatg
     9541 ttactggatt ttacccgtta cctgttacca aaacgctaga acccttgtcc ccgttgggat
     9601 tgagtggtaa cgggtacctg ttacgtaggt gttaccacgt gttacgaatc aaggaggaaa
     9661 gtaaatgctc aatattattt caacaaacaa agcaccgaac tttcaatata cagatgaaat
     9721 ggatcggttt ctaatgaaca cgctggcatt tagtgtggga ttggtaacag aagattattc
     9781 cacatttgat ccagaagtat taaagataat ggaagaagaa cctgattggc tgcaggagtc
     9841 agtagcgtgg tgtcaatcgc tagtagtggg atcattggta gatagtggca actatgatga
     9901 tacaggtgag ctaatggatg aatttaattg tctgcttaac ctatatgatc gagcgagaca
     9961 acgagagctt acatcgaatg aggataattt gtttttaaac atccatgata agttcttggc
    10021 tttgctacta acagatgatg aattgataac taatttattg gaggtagaat aattatgcat
    10081 atggaaagta ttagatactt cgatggtgag aaacacattg agtattcaaa cactcaagaa
    10141 gatgtagact ttattagttt tgaggaagat aaaatcacta aagttaattt taaagacgga
    10201 acgtgcttga agatagtttc gccttatatt gaatacaaat caaaatggga acaagatgat
    10261 tctgacgaac gagatcgctt tggttggtaa tctatggcag ttaaaaaaca atgtaatcat
    10321 gctggatgta aaacattgat tgattatagg cagaagtatt gtgggaagca taaagcaaag
    10381 caaacggcaa ttaagcgtga agaaagaaag caaagcgaag gtaaatattt tcaattctat
    10441 caaagtagaa catggcgaaa ggcttcatac ttgtatcggt taaatcatcc agtatgtgag
    10501 gattgcctag aagaaggctt gataaggaaa gctgatgttg tagatcacaa aattgaacta
    10561 aaagatgatt ggaatagacg gttagatgaa actaattttc gttctttatg ccatgcacac
    10621 cataattcca agacagccaa tgaaagacaa agacgagaaa agagtaccct ttgagttaag
    10681 ggggcatagt gtgaaactta ctgacaatcg atgcctactc attttggtac aaataaccgt
    10741 tgtgaaaagg cataagggta attacaaata acgattacac ttgtaatcac aagtgaaaaa
    10801 gtatataatg aaagtaggag atttattgaa aaatacaatt gttattactg ataaaacagg
    10861 gaaacagcgc accattgagt tacctcattt tggtcaagtg accattcaaa tgcagaacgg
    10921 aaaaatcatt tatatagata aattagataa agaaaaattc tgatcgaaaa acgaaggaat
    10981 gtgagcttaa ttgcttacgt tcctttttct tttgtctgaa aggaggacaa catgggacac
    11041 cctaaaatat tacacgatac aaaaggcaat atatcaagtg aagaaaaagc aatccgtgtg
    11101 gacgctagag aagaattgtt caagcagcaa ccattaataa atatcacgcc ccctgactgg
    11161 atggcagcga gcgctagaag tgaatggaat cgtatcgtac caacattaaa aaaagattat
    11221 ccattgagtg aagcagatta tggttcattg gtagcctatt gtttagcctt tgctcgaatg
    11281 aaaacagccg aagccgagat aagaaaatca ggtacgttta tcacatgcga aaacggagta
    11341 aagaaagcca atccagcggt tcgggttcaa tcacaagcta tgagtgattt gaaaaaacaa
    11401 gccacctcac taggtatgac cttagaatca cgatcaaaac tagctttgaa caaggttaaa
    11461 aatgatgaac ccgaagaccc gttcaaagag ttgatgaatt catgaatgat tacattgaga
    11521 aagtcctatc aggtaagctg attgcaccca agaaaattat ccaagcgtgt gagcgtcata
    11581 taagcgattt ggagcgttct aagtcagata gcttccctta tgtgttcgat gaagaacaag
    11641 ccaccaaagc gattaaattc attgagttgc taccatctac ggacggtaaa gcaatcaaga
    11701 tgttaggatt tcaaaaattc attctaggca gtctttatgg ctggcgtact aaagaaggga
    11761 attacaggcg attcaatcga gcgtttacca gtatgagccg taagaatggg aaaacgtata
    11821 tcgcaagtgg catggctgcc aatgcgttga ttatggaaca agaaccagca gaagcaaggc
    11881 aagtattgtt tgtaagtaac gctttgaaac aagctaaatt gggctatgat atgctgtcta
    11941 attcacttag aaacgtggtc aagtctagta agtttttaag accacaactg aaaattatga
    12001 actctaagat tcagcacttg ccctctaatt cgttcgctat ggcactggct agtgaaacca
    12061 gcacgctaga tgggtttgca ccaacaaccg caattcttga cgagtggcac gaagcaaaaa
    12121 ctcgtaaaac gtacaacgtc attaagtcag gaatgaccca acaaaagaac ggcttattgt
    12181 gtgttattag taccgctggg cttgatttaa acgttcctat gtacgaggaa tacttattgt
    12241 tagatcgtgt gctaaaaggc gaagaacaag ccgacaggta ctttatagcg atatgggaat
    12301 tggacgatcc cgaagaaatt cacgatcaag agaaatggat caaagccaat ccgatttttg
    12361 aaagtgaaga aatcaaaaaa gtaatgattc caaccattca agatgatgtg aaccttgctt
    12421 tgaaacaaaa taaccttaat tctgtattgg tgaaaaactt caatttatgg agacaagcga
    12481 gtgaggacag ctatatgatt gctgaagact ggcaggcaac cgaagtagaa ccacaagata
    12541 ttacaggcaa gcccgtttat atcggagtgg atttatctaa aacagatgat ttaactagcg
    12601 tttcatggat cgtaccgcta gataacggcg aactttattg tgattcacat agctttgtag
    12661 ccaccaaata tgggcttcaa gacaaagaaa agcgcgacgg tttgccttat cgagaactag
    12721 aaaaagctgg tgagtgttcc attactcaat tagaaagtgg aatcgtggac tatgaccaag
    12781 tatttcagtt tattcaagat ttgattcaag aaaatgattt ggaatgtatg gggatttgtt
    12841 atgacccgta taacgctaat tcgcttatca gtaaagctga aaaagccaac tacccaatgt
    12901 tagaagtaag acaaggaacg attactctaa acgttccgac ccgaactttt agagaacaag
    12961 tttatgaagg caacgttatt cacaataaaa atacgattct cacccatgca gtgaacaacg
    13021 ctattttaaa aacggataac aacggcattc agattaataa atcaaagaac agtaacaaaa
    13081 ttgatccaat agctgcatta atcaatgcct atgtgtttgc aatggattac ttcaccacaa
    13141 cggaaggagc gaaagcagac aatgaatttt atacaagtga agaattttct ttctaattac
    13201 attcataccg ttcttttact tcttggattg gtgtgtgtgt tggttgctat caccttacta
    13261 acaaatgtct actatggctt gttagcgctg ggcatagtgc ttattggggt agcggtcatg
    13321 ctaaatacag aacagaaggg aggttaaaag atggcatttt ttaaagcgag acaaaataca
    13381 acgggagatc ctttcctaca acatgtggta tcaatccaat cggatgatta caccaccagc
    13441 tttacaagcg ttcgtgcatt aagaaatagt gatgtgtttg cagccgttcg aattattgct
    13501 agtgatattg cttcaagtcc gattcaattg gtcaaaaaca atatgccaca agctgataat
    13561 gaactggtga agttactaaa cgagaaacct aattcagaaa tggatggttg gcatttaaag
    13621 tttgctttgg cggtcaatat gttgctaaac ggtaatagct ttgcagagat taaacgcaac
    13681 ggtgaaaagg tagaagaaat tcacttatta cctaactcaa gtgtaacggt tactcaatta
    13741 gataatggta cgttgtctta tcagattggt gataaaaaaa gacgtgtgaa gtctagcgat
    13801 attttgcact tcaaatattt cacccaagat ggtttaacag gattaccacc actttatgcg
    13861 ttacgtgatg aactgaaaat tcaacaagct ggcaaccgca cattgcacaa tttctttact
    13921 cgtggtgtca gtgggtcagg tattttgaag gttcataagt cagatttaga cggatcagca
    13981 aagaacgcaa ttcgtgaaaa gtttgaagaa gccaacggtt caagtagtgg agataatgct
    14041 cttagaacga tcattcttga tgaaacaatg gactacaaaa cattagaaat aaacaccgat
    14101 gttttgaagc ttatcaattc caatgattgg aacacaaaac aaattgccaa ggcgtttggt
    14161 gtaccgattg agcgtttagg cgttgaaaat gaacattcta gcaccgttca aagtaatctc
    14221 cagtatatcc aaagtacgct gattcattac tttaatgtgt ttgtgagtga attagatacg
    14281 aaacttgaaa ccaatatccg ctttaattcc gatcagttac tagagactga tcctgaaaca
    14341 aaagtaaaaa atgtattgga tcaggtcaaa gggtcacttc tcacgattaa tgaggggcgg
    14401 tcgaaaatgg ggctaccccc catggatggt ggcgatcgtt tactagcaag tttgaacttt
    14461 acgtatttag atacgttgga gaaatatcaa ttaaaagaac aggaaggagt tacaccagtt
    14521 gaataatgaa gaagaaaagg aaaaacggct gacagaagaa gctgagctaa cagccgattc
    14581 tcccaaagta gagaaagaaa atgaaaaaca accaacagac ggcaaaacta tttcaggcta
    14641 tgcgttgaaa ttcgggcaac cgtcaaaaga tttaggcggc tttgtagaag tgattacacc
    14701 cgaagcatta aaagaggtgg atttatcaaa tgtgttctta ttatatggac acgattattc
    14761 aaagccacta gctagtgtca aagcaggtac gttgaaattg aatgtcgatg atacaggatt
    14821 acattttgaa gcagagctaa ccgacacgac ctattcaaat gatgtgtatg agaatatctc
    14881 aaaaggggtt gttgattcta tgtcctttgg gttcgtgctt ggattagatt cattcgacaa
    14941 aaaagaagat ggcacaattg aacgatcaat agataaaatc aaagcactta atgaaattag
    15001 tgtcgtgacc gttcccgctt atgattcatc aaatgtccaa gtaaataagc gttcttacga
    15061 atcgtttatg agtaacaacc aagcaaaaca aacaaacaat agcttagaat ccacttctaa
    15121 agcacaaaag gagagtaata acatggaaaa aactttaatc gataacgaaa aaacagaatt
    15181 gcgtgggtat gaagaatata tccgttcaca aggcgaagtg cgtgatggag tcactactgt
    15241 aaatgcagcg gcagttgttc ccgaagaagt aatcggtgaa gtctttgatt tgaaacgttc
    15301 aaattataac ttagctcaat atgcaacagt aaaaacagta tcaaatggac aaggtaaata
    15361 tccagtagca actaaccaac aagcagtgtt agcaacaaaa gctgaacttg ctgaaattgg
    15421 tgatattgac gctgaaatgt ttacttcagt tgattataaa gtagaaactc gtgctggtaa
    15481 gattgcctta tcaaatgagg ttgtggaaga ttcagcagtg aatattgtac aagaggtcaa
    15541 agatcagtta gcgaaattgg tagaaaacac tgacaataag catatcatgg atttattaaa
    15601 aacattcact aagaaaacgg ctgctacgtt ggacgatttg aaacaactat acaatgtggc
    15661 attagaccca gcattaaata aaatggtaat tctaaaccaa agcggatatt accacctaga
    15721 tacattgaaa gattcagatg gacgttacat tttacaaccc gatgtgacag cacctagtgg
    15781 taaatcatta ttcggtatgc cagtagtatt gattgcagat acattgtttg ccaatcctaa
    15841 agctggaaca ttccctatga ttatggggga tattgcacaa tctatctttg ttgctcgtag
    15901 aaatcaagta acgactcaat gggaaaaatt cgattactac tcacaaggac ttgcagtgat
    15961 cgttcgcaac gattataaga aaattgatca aaatgcttca gtgtatattg agtttacgcc
    16021 agttgtaaca ccaaaagtat agaaaaatat tgggcggcgg tttatcccac tgcctttttt
    16081 tcattaggag agataaaatg gtaattttgg atagcataaa aaaaagtatg cggatcgatc
    16141 acactattga tgatgacttt attcaacaat tgattgatac agcaggcgaa tatataaaaa
    16201 gtgctattga tagtagcgca acggataaag atatggataa ttatcagcaa tttgatttgg
    16261 cggtgtcatt acttactcaa cattggtatt tgaaccgtca agaagccagc agcgaacgga
    16321 taccagtaac ggtacaagca ttagtacaac aaatgagagg tgcttattat gccaatcatt
    16381 aagaatgtaa atgaattgac tgagagagtc aagtttaaaa agacaaaacg ggtaaaagat
    16441 gaagatgggc agatggtgga tagtgaagaa actgtatttg aatgttgggc taatgtgcgt
    16501 tcacaaatgc taaaagacgt gcttgctagt gtaggtacca ttcttgaagg aacgttgacg
    16561 tttattattc gatacgatca agattatgaa ctaacaaacg atatgaaagt agcttggaaa
    16621 aacaagagtt ataaaattat ttcaatcaat gaaggaacgg cgtttaaaga ttatacaacg
    16681 atcatagcta aacagatttc ttagaatgat tacacttgta aacgttgtgg gtatttagta
    16741 taattaaggt agtaaatgaa gggttagctg cctgaatttt gcaagactta gctagtcgaa
    16801 atctattgtt ggactgaaaa ttgtaggtgt ggttgcaaac atgctggact aagaaagatg
    16861 atagatgatg aacgttcaat tttaatacag gcatgctagg actgattgga tggttttggc
    16921 aagctgattg tgtagaaatc aaagaacacg tctgttgtgg acgtgttttt tttgttataa
    16981 ttcaaaagag gtgatttaat ggacaaagca gagtttacaa atttttattg gaagtattat
    17041 ctacatttag aaaatgagtt tattaatacc acagattttg tgatgctcga tgagaataat
    17101 ttcaaaacat tctctattga atatcaaaaa ctactactag cgattggttc ggagtgtgag
    17161 attattttca aagaactttg cggatttgat agcagtagca ataaaagcat aactgatttt
    17221 aaagctgtta ttaaatctag cgaattaatg tgtttggata atggtgttag ggttatgaat
    17281 tctttaacat tgacttcact aaaacctttt ggagatgggt ggcctgaaga tacaccagat
    17341 tggtggaaaa tatataacag agtaaaacat ggtcgttctt caaattataa ggaagcaaat
    17401 ttggaaaatg tgttgtatgc tttagcaagt ctatatttat tagaggaata tttgcataag
    17461 aaagtaatac tagaaggtga agtagacttt ataagtccag agtcaaatct atttacgtta
    17521 tcttgggaaa gaaaacattc gtcgatgcaa aaagtgacgt ttgaaaaagt ggattcaaat
    17581 tatgtagctc cactcattga ttttaaagaa caagaagaaa tttaaaaacc cccacggctt
    17641 attgaagttt ttgtatgcca tattgtatgc catttggttt aaatcgcttg aaaaatagtg
    17701 atagataact ctaattcatt tagtcataaa cgctgttttg ataaccttta tagttctttg
    17761 atatgggttt ctcattcatg gcggaagaag aagaatagaa caaaagaatg aattaataag
    17821 atataaaaaa agggattgtg acaaacgtct tgtcacaatc cctttttcca aataaacgat
    17881 gttcaatcag ctgcccctcg gcattcgctc gaaaaacggc gaaatatgaa gaactatttt
    17941 gccatttgtt tctctaataa tcaggtcaaa acgttgactt cacagtctct tccttaaacg
    18001 gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat gagaagctgt
    18061 tttttgaaat tgttccttgt gactcggagc tgaacggcag gctcacgacc tctttcttaa
    18121 acggtgttca aaagcttgca tctgcggtaa taagttcaac caaacgaaaa ttatgaggag
    18181 ctatttttgt ttggctgttc ttattacttg atgcaggaca gctttttcac aacctctttt
    18241 cgtttagcac taaatttcct taaaaagtag aaaagctttc gaagcctcgt ttgtttcttt
    18301 tactgtataa taaaagaaaa gagtaacaat cgctaaaaga tataaacaag agaaaggaaa
    18361 tacaagatga ttggtctgat ttattttgtg gtgatcgttc tggcgaacac agttggcgct
    18421 atttccggga tgggtggagg cgtgttgatc aaaccgattc ttgatcttat cggcgcccat
    18481 tctgttgcag ggatttcttt ttactcaaca gttgcggtat tcacgatgtc cattgtttcg
    18541 actgtgcgac aagtatcaag cgggaaatca ctgaattggc agattgtcgg ttgggtatct
    18601 agtggagctg ttgttggagg agtagtcggc aacattgtat tcgaagtttt tttacagtta
    18661 tttgaaaacg agaagcatgt gcagctgatc caaatttttt tgacggtatt gaccttggtt
    18721 tttgcgttct tctacaccaa gcatcatcag ccaaaatttc atttgacgtc atggacttgg
    18781 taccttatat gtgggggggt tctgggcttt ttagctagtt ttttaggtat tggtggtgga
    18841 ccagtcaatg tttctttgtt gatgctgatg tttgcattgc caataaaaga agcaacacta
    18901 tattctctat ctacgatttt cttttctcaa ctggcaaaat tggtaacgat tgctttaaca
    18961 agtagcttta tgcgctttga tttatctatg ttgttttatg ttatccccgc tgctgttatt
    19021 ggaggtttgt ggggagcaag atttagcagg gttttgtctc caaaaaaagt gacctttatt
    19081 tttcaagtaa ttgtcctcgt ggttcttttg atcaatcttt acaatgcttt cgtcatctta
    19141 caaacattag gagaatctag actaacgcaa gtcaaaatca ctgaaacata ttgttctaaa
    19201 tgaaaaaagg agtatactgt gataaaaaaa ttcatttaaa aaaataaaaa ttggctggtg
    19261 atttggatgt tgcgggaaat gcgtttagga gatgcagaag cactacgaga aatcaatgcg
    19321 gaacaattag gatatgatgt accattaacc gttacggtga gacaaatgaa gaaactgttg
    19381 aaggagccag aaaaaaattt tttcttggtc tatgaagatc ctacttctaa aaaagcagct
    19441 ggctatgtac aagcggagtc ctatgaatcg attttcagcg atccgatgtt caatattatg
    19501 gcacttgctg tgtctaaaca tgctgaaaaa agaggaatcg gaaaggcctt gatgactggc
    19561 gtagaagaag cgaaaagacg aggaatatca gcaataagac ttaattctgc agaatacaga
    19621 gtagaagcac accgatttta tgaacgcatc ggttatcatt ccgataaaat gcaaaaaaga
    19681 tttttgaaaa tcctgtaaat ttttatcaaa aaatacctgg tcatcaagcc gatacttgat
    19741 gatcaggtat tttttttgac taagaaagta attgcatcaa gaaacctgag atgatcaaaa
    19801 cgatggcacc accaagccga ttgcccattt gagcaaaagc aataagttcc atccgattag
    19861 ctgcagataa gacagctaca tttcctgtac cacccatcga gttgttgcat aagccagcag
    19921 taatagttga ttcgattgga tataaaccaa acaaacgacc gacaaatcct gaaactactg
    19981 aaatcgcgat aacacttgtc aaacataaga caacaaattg ccatgtcaaa gcagaagcca
    20041 atacatttag attcaataac gcaataccga taccggcaag aacagctgct gtcaagtttt
    20101 tgacaatcaa gttgttgaac atgatagctg catcttcgta atattctggt aataggtttg
    20161 cgattttaca gataacaacg ataatgatca tgaatgcgta agcatggact ttaggaacaa
    20221 aatagttaca gatttgtcct aagatgaaga aagtcaatga taccatcaaa ccgacaccta
    20281 attgtggaat ggaaggtttt acatttctag gttttccatc gccaaggtcg cctttttcca
    20341 ttaatttacc attaccgttg aattttggca ttgattgtcc cgcacgagca actaacgctg
    20401 caccaatgat agctaggaca tttgtcattg ctgtcgcagg aatcaggcgg gaaatgatag
    20461 aagaagcgtc cgtacctaaa acatttgcat aaatcgttga taaaggtaca gctccggctc
    20521 caacaccacc agccattgcg ggcaatgaga tatataaaac ggaatttgca aaaccatttc
    20581 cgataagcat acctacgagt cctacggcaa aaaaggagac gaccattgaa ataagtgcaa
    20641 cgggaataaa acgaacagaa gctttcatca atagattacg gttcatacca aggatgctac
    20701 cagtaatcaa ggcagcaata taaaagtcta aaaagcccat attgctgaca aagttttctg
    20761 tggattttac tacatactca ggtaagatcc caaatgtagc aatcgcggca gaggcaaaga
    20821 tcgcaaaaac ggagccacca cctaaataac ttttgacgat agggattttt gttccgataa
    20881 aatgaaataa attaccgaaa atgaccaaaa cagcgattgg gccaatcata ttcgtcggta
    20941 attttcctaa agccattgca atgatcagga caaggaccat gatcaaataa aggggcaaac
    21001 ttacgccggt aatttttgtt gaccaaaacc cttctttttt cttttcctca actgcagttg
    21061 cggttgttgt tttttgtgcc atttttacag atcctttctt attctgtatc gtatgaaaaa
    21121 agccaaagag aagcggcaaa agagcagtag ttggctggct gctgttttgc cgcagtcgct
    21181 tttttctttt tagacgtcat acaaagtttc tgtatgttct tttactataa caatcttaat
    21241 cttctaaaga tttatattca ggaacccatt tagcagattc aacagcttct ttgatatccg
    21301 tgatttcttc acgattcaat ttttgtgcaa caactgattt tgcgacagtt tcagcaatcg
    21361 tttgagagaa ttcagtgatt tttgctactg gaggcaagat agctgctcct ggttttgtta
    21421 catcaacgat tccgcctaat gctcgactag cttgagaaat gatttcagca tttactcgtg
    21481 tagctgttga agcaatcaaa ccaagcccta aacctgggta catcaacgca ttatttgctt
    21541 gaccgatttg gtaagttaca ccgttatatt ccacatcagc agcagggata cctgttccaa
    21601 ctaaagcttt tccgtccgtc cattcaatca aatcttttgc ttttgcttct gccaattttg
    21661 ttggattaga caaaggcatg atgattggac gtggggtatg agccgccatt tctttcacga
    21721 tttcttcagt gaatgctcca ggttgggtag aagtaccgat catgatcgtt ggatggattg
    21781 cttttacaac tgcttctaag tttgtcaatt catcagcatt agtgaattct gagcgtttac
    21841 gagcgaatgg gatttgacca ggagtcaatc cttttgtatc ttcaaacaat aaaccctgtt
    21901 tatctacttg atagaaatgt tggcgtgctt cttcttcagg tacaccttga cggatcattt
    21961 catctaaaag gatgtttgcg atacctacac cagcagtacc agcaccaaat gtcaagatcg
    22021 tttgatcttt caattgctca cctgatatat tcaatccacc taatacacca gctagtacaa
    22081 cgattcctgt gccttggata tcatcattga aagtagtgat tttatcttcg tatttttcta
    22141 agatcgttgc agcatttcct cgtccaaaat cttcccaatg taataacaat tctgggaata
    22201 attcagtagc tgattgaacg aattgatcaa tgaattcgta gtaagtttcg ccttctacac
    22261 gagcatgacg attccctaag tataatggat tattcaataa ttcttcgtta ttagtaccag
    22321 cgtcgattga tacaggcaag acttgtgcag gattgatacc agccgcagca gtgtagacca
    22381 tcaatttacc gatagcgatg tcaacaccgt tcacacccca gtcacccata cctaagattc
    22441 cttctgcatc tgtcacaacg atcaaacgaa tgtcgcgtcc atctgctgcg tttttcaaac
    22501 ttgctttgat attttctggt tcgtcgattg aaagaaatgc agcatcttgt ggtttcaaga
    22561 aaatttcgtt gtattgttcg attgaatccg caacgacagg atcataaacg acaggcatga
    22621 attcaactaa atgttcgccc atcaatttat aaaacaatgt ccggttcgta ttgaatagat
    22681 tcattaagaa gatacgtttt tccaaatcgg tctgtttgct taagtattga ccgtaagctt
    22741 ggacagattg ttgttccaat gtttggacag tacttggaag cataccagtg attccgtatt
    22801 ttgcccgttc ttcttttgta aaagctgttc ctttatttag aaaaggatta tttaataagt
    22861 ttagccctgt aagcatgaat gattcctcct aagttttaat acaaacgaag tataggagtc
    22921 agatgaggat atagtcaaaa agagcaggta taataaattt tatttatatg agtaatagag
    22981 tcaaaagaaa tcaaaatagc ggtatttgtt ctttttatat caacaatcct tgcttgatta
    23041 ctatgcgtat ctcttatatg ctaaagaaag aaaagtagaa gaataaagga tatgttagat
    23101 agataaatag aatctctgaa gaataaatca cgtgatcgtg acgataaaat acacttacgt
    23161 caggtgataa aaaatacata agtagaaaac taagaggagg aacagaaaat gaaagaagtt
    23221 caattgatca gaaaaagcga actatcagaa ggtggttgca atgcatgcgg cgtagtagaa
    23281 gcaacaagct atacattaaa gcttggtgcc aacaaagcga taatctctga gttgacagta
    23341 ggcggtcttg tggacagtct ggcgcttgct gaaggtttta ttggagaaga tatttatgag
    23401 atgttttcag aaatccgtca gttaaaaaaa ggggaaaact gcatagaggt tcatcatgag
    23461 agcccgaatg tgagattcaa acgtggtgat aatgagatga tcttcaataa tcatgtgtcc
    23521 gatcacaccg agctatatgg aatcgtgaat caaatactaa ctgagctttt tggattggga
    23581 ccttatgcgt tcaaggaaga aaacggtaat ccaaaattaa acgaagaatg gcaagaaacg
    23641 attgaaatac aaagaaacaa cccgcactta tttcaatagg catccacagt atttcgtaaa
    23701 agggactttg acacgacttt tgtcaaagtc ccatcgtttt ttatctattt gtgttctaaa
    23761 catttctttc agagtagtaa caactggaat gactgaaatg taaacagatt atttgcctgt
    23821 atcaatacgg aagagtaata taaagtgtgt aaacgctatc atattattca ttcaattttt
    23881 tatagaggga aggcattgaa atggaaaaaa cgtatcaagc aggtacaaat gaaggaatag
    23941 tggattttat caatatggaa gatttagaaa tagccgcttc acaagtgatt ccagctggtg
    24001 gttatggtta catcagcagt ggtgctggcg acttatttac ttatcaagaa aatgaacggg
    24061 cattcaatca tcagctgatt attcctcatg tattaaaaga tgtagaatta cccgatacaa
    24121 ctacacattt tgacaaagaa acgctgactg caccaatcat catggcacca gttgctgctc
    24181 atggcttagc tcatgtgaag gcggagaaag cttctgcaaa aggtgtagca gattttggga
    24241 cgatctatac agcaagttct tacgcttctt gtacattaga agagataaga gaagcaggcg
    24301 gggagaaagc accgcaatgg tttcaatttt atatgagcaa agatgatggg atcaatttgg
    24361 atattcttga agtggccaag cgtaatggag caaaagcaat cgtattgaca gcggatgcaa
    24421 cagttggcgg aaaccgtgaa acagaccgca gaaatgggtt taccttccca ctgccaatgc
    24481 cgatcgttca ggcttatcaa tcaggagtag gccaaacgat ggatgctgtg tacaaatctt
    24541 ccaaacagaa gcttagtcca aaagatgtag aatttattgc tgctcattct gatttgccag
    24601 tctatgtgaa aggtgtccag tcagaagaag atgtctatcg ttcattagaa tctggtgctg
    24661 gcggaatctg ggtatctaat catggtggcc gtcaactaga tggtggtcca gcagcgtttg
    24721 attcgttgca atatgtggca gaagcagtag ataaacgtgt accaatcgtg tttgacagcg
    24781 gtgtacgacg cggacagcat gttttcaaag caatcgcatc aggtgcagat cttgttgcta
    24841 ttggcagacc cgttatttat ggattgtctt taggaggaag tacaggagtc catcaagtgt
    24901 ttgatttctt taaaacagaa ttggaaatgg tcatgcaatt agctggaaca caaacagtag
    24961 aagatatcaa aaaaataaaa ttgcgtgaga atcgttttat ttaagaaaca aatgacgaaa
    25021 aaaataggag tgcggcagat gatgccgtac tcctattttt atagttcaag gatgtcacta
    25081 atagaataat ttgaaacgaa aaaataaaat tgaaatattt tttcatactt aataaaaaag
    25141 gaagaatatt catgtaaacg attttattgt ttttttctct tttatacaaa aagggtcata
    25201 atttcaagta agcggatgaa ttcatgagga ttttttgcat actgtctcta ctatacttag
    25261 gtcactaaga aaaaggagtg atttattatg aaaaagaaca tgattgggac attattactt
    25321 tcaacacttc ttctaggcgg cttggcaaca ccagcttttg ctgagggtca agcgacttca
    25381 aaaggagata ttacctttac tgaaccaaca aataccgtgg agcctcttaa ccctactgat
    25441 ccttcaaaac cagtagagcc agcagatccg gaaaatccag caactggcca gacaggtcct
    25501 ttaacactgg atgtagttcc cgaattgcca tttggtacac acgagattga aagcggaaca
    25561 aaaacgtatc aagtagacgc ttcaaaaaac gatacacctt atctccaagt atcagatcgt
    25621 cgaggtgtag gtgcagatgg ccaagcacaa gggtggaacg tgacagtttc ggtttcagat
    25681 tttgtaaacg gttcacaagt tttgcaaggc gctgaattag attttggaac atccactgtt
    25741 aaaagtacgt cagataacga atccactgga ccaactagtc aaactgtaac tgggttgagt
    25801 aaagcaagtg cagcgacacc aatatttaca gcagcaaaag atcaaggtct gggaacatgg
    25861 ctttctgttt atgatccagc aaatatcaca ctgaaagtgc cgaaagcagc agctggaaca
    25921 ttcacggctg atttgacttg gaacctcgtg gctggcccag tagcatagag tatgaaataa
    25981 attcaacaaa ctgcggtgaa aatactatag gtgaaggatt ttttaccgca gtttttgttg
    26041 aaaggatgtg gtggacaatg aatcaaagta aacgactgtt tgtttcgttt ttttctattt
    26101 taagtctatt tttcattttt ccgtcaatca gccaagcaaa ggattcagca ggagactttg
    26161 gaatcaaacc ggtatttcca gagaaccaaa tagataaagc aatcggttat tttgatttat
    26221 tagtggcacc ggaacaaaat cagacactag aagtaattat cagcaattct tcagatgaag
    26281 aacgaacatt tgaggtctct gtcaatccgg cggtaacgag tgatggtgga acgatcgatt
    26341 atagtcaaaa aaacccaacg ttagatgaga cattgccgtt tgatgtccgt gacgttttgc
    26401 tcattgcgaa aaaggaaatc aatgtatcag cacatgcaga aacaactgta cctatagaag
    26461 tgaaaatccc agcaaaaagc tttaagggac gtgtattagc gggtattcat gtttcaccta
    26521 aagaagaagc tgaaacagaa aatgctacag aaggagcaca aatcaaaaat cgtattgctt
    26581 ataatctcgc agtcgtttta caagaaagtc aagagacgat agaaccggat ctgaaacttt
    26641 tatcaggaga tttggacgag gtcaacgcaa agccaactgt tcagcttcgt ttccaaaatc
    26701 ctcagcctag gattatcagc aatttgatat ttacgagcaa gatcttttat gaaaatcagc
    26761 tttatattga aaatacgtct aatgcatttt tagttgcacc aaattcgaat tttcacttga
    26821 atcttgattt agctggtgat aaagcaaaag ccggcgatta ccgcgcagaa atcatagcaa
    26881 aatcaggcga cagtaatgaa tggcgattta cacaaaactt tacgatcaaa aaagaaaaag
    26941 cacaaaaagt aaatgaaaat tccgtttttg cagtacaaga acaatctttc ccatggcttt
    27001 atgcaggatt ggttgctgca gttctagtgt tatggttgat tttgctgttt ttgtgggtcc
    27061 acaagaaaaa gaaaaaagga gaaacaacat gatgaggtga tttcgttgaa aacatcgatc
    27121 ttattgggag ggattattgc aagtttgctg ttcttcagct ggcaagagcc agtccaagca
    27181 gataccaaaa gttcaggtac gattacattt tatgatctaa gtccaattcc gcatccaagt
    27241 ggttcagtca atggcgcatc aaaacagcag acacagctac cgatttataa agaatctaat
    27301 caacatggcg tattcccgga gacgaatgaa caaaagatcc gttttcttta tttgatcatt
    27361 ggtatagaat tatggcttct tgcgtggttg ttgttatgga gaagaaagga aaaggaggat
    27421 gaacatgttg ctacaaaata aattttcatt ttttcgctcc ttctttttta cttcgctttt
    27481 tgttttaagt atatttatct tttcaccttc ggcagaagct gttggagcaa cgcctcctgc
    27541 catttcttat gatccataca cgtatgaaaa tatcgtacct ccaaatggtg gacgtagtgc
    27601 agtcaatggc gggaagcttg tccaactaac tggggctaac caattgccta aattgactgc
    27661 tacttatttg aacgatggtt caaatgatcc ttggaaaggt ggcacggtcc gttttcattg
    27721 ggaaagccat gtgaaactaa caaatgtact tgatttggtg aatatcactg cacaaggatt
    27781 tgcatttgtg gcgatcatca atttgccgga acatgtatca agcagtgatg tattaaaagc
    27841 aattgactgg aacaacgcct acttgtatat tgccggtgcc cccatcaagg ttactagcgg
    27901 aagtttatta tcagcaggaa gccatacctt gcgttttggg atggggtcgt gggatacaaa
    27961 tactctattg acagatatta ttgctattat tacgcgaggc gaattcaacg tggacgatat
    28021 cccgcttcaa tttgatgtta cagctaatgt cgcgaagatg acagaaaacg gagataaata
    28081 tgatacgagt ccaaacaaaa ttttgaccaa gggtaaatta caaccttcta tgtctaaaaa
    28141 ggccgttttt tctgttgatt tttatgattc gaataatatt atccaagatc gtatgttcaa
    28201 ttcgatttta gctccagctg caggtagatt ttatcctaaa ttaactggcg gaaacttgaa
    28261 tccaggagca tatgcatttt ctgcaagtgc agagataaat acgtggaata gttacatctc
    28321 accaagtgat tcaacaggaa aatataatac atttgctgat accacagaat acgtggatgg
    28381 aaataaccag aaccttccag gctctaccaa tagtcggaca ttgacgatgg ggctgactga
    28441 aggacgtttt gatcagttgc catttagtcg ctttgatcgc gtagtcaact attttacagg
    28501 aaaaaacgtt acacaaggag caatactgac tcatactcca gcaagcacaa gtggattgaa
    28561 tcagacagcc aatatcgtgt acagtgggaa agatgcgcaa ggcggattgc tttctcctgt
    28621 tgctttaaag gtattggagc agtacaagct agacggaacg gttttaccaa caaacttaac
    28681 agccgatagt cagtggggaa agctcctttc ttctgcatcg taccaagtca cggccaattg
    28741 gtcagcaact gaactaaaaa agggttcgat ccattacagg ctttattcga aaggaaccca
    28801 aaaattagcg acaggatgga cagatcaact tttccaaacg atccaatcaa ataacggggg
    28861 tgtaaataca gctacttcta cactacctgc attggcttca gggcaatatt attttgatta
    28921 tcgcttggtg gacgatgtac ttactaacat gtatccgtct tttgcgtaca aatggcagtc
    28981 tgaacaagct ggaatcacca ctcttcctca aataacagtg gctaattttc catctgtgag
    29041 tgcagatagt cagctaaaaa accttagcag acaacctgag acaggagatc ccctgtctgc
    29101 tctatcagga gatacgattc aagaaaatgt gacattcacg ttaacaaaat ctggggatgc
    29161 gataaccgac aaaaaaatca cgatctcact tcctgaaaat actacttata tcaatggttc
    29221 cttaaaatta aatggtacaa ctatcgcaga taccggcatt caacaaggag tttctgtccc
    29281 agcagatcta ttgagcaaaa taggagatac cattcatctt acttacaact atcaattaaa
    29341 tactgtagat acttctttac aatccgtcag catactgaca aaggcagctg tactttccag
    29401 caatattaca ttagcagatg gtgctaagct tcctaaccca gttgtccaaa catctgctaa
    29461 aacaattctt gtccccaaac aagaattaac attagttaat gtaccagatg attttacatt
    29521 tggtaacgat ctccccaaac cgttgaagac aagttattat gaatcaaaag gcgatttttc
    29581 ttttgatgtg cgggatactc ggcttccaag cacgtctcct tggcaactga caggtacatt
    29641 gacttcttta tttaagaatg atcaaggtca agaattaagc ggaacaaaat tatacttcaa
    29701 tcattctgga tcaaagcaat tgattcagca aggtcaaaac acgttgattt atgagagtga
    29761 tggaacagct aaaggagagg ttctcgttga ctttcccgat accgatggtt tactcttaga
    29821 ggtaaatagc agtacgaatg ctcaacctgg cgcaacgtat caaggcatgg tcacatggaa
    29881 actgacagct gggccaacca gctaagtgat tagaatttgt atggatctgt gataactgtc
    29941 acagatcctc tttttcaagg atgagaagga aacttctgct gttaaaatca gaaatgcttg
    30001 aaggagtgga cttggaactt ttcagtttgc ttgaaaaaaa tgaacaacgg caaatcaaac
    30061 ttttccaact tttattagag gaaaaaggag aggtttccgt taataacgta cttacccgct
    30121 tatcagttga tcgggctacc ttgaaagaag atctgtttta tttacaagct aacttggaaa
    30181 atatcaaaca attggaaatt gttatccaag aagggaatat tttattaatc agacatggaa
    30241 atatagcagc agtcgatgtt tattatcatt gttacttgcg ctattctaca aaatatcaga
    30301 tacttcttta tctgttggaa aatggctcat ttgatcggca gaaacttgtc atggaactta
    30361 atatcagttc ggctactttg tctcgcagag tgaaggaatt aaatgattct ttaaatgagt
    30421 ttcatctgaa aataaaaagc gggcggctga ccggtccaga aagtcaaatt cgttattttt
    30481 atttccaatt attttggttt ggacgtccct atcttgaaaa caaaaaagaa tacagtgatt
    30541 tatcaggaaa cactttttta gatctgctgc aaaaagagtt tagttttgct attacagaag
    30601 aaggacgatt gaaatttgaa atatggcttt ccattatgaa gaagcgaatg cgctgtacga
    30661 aaaaatccaa accagattat agcaatcttc ctgttggatt gtttcaaacg acacaatttt
    30721 tattggacct ccatacattt ttgacccgtt acttctttca atatgccttt ccttggaatc
    30781 aaaatgaaac gttggttttt tatcttttca tgatcagtaa ctttacatta gatacacata
    30841 actcgcatgt ccaggagttt ttgataagcg aaaaatacaa agacgacgta gtagctcaat
    30901 tgaatcgaac gtttaaacaa tcgatagaag aatatttctc tttttatgtt tttccagaag
    30961 agatttcgca aaagattgcg ttgacagtga tgcaaaatca cgctcatttt gcctatttta
    31021 gaggatggat gaatattttt ggccaagaag gattaaaaca gcggttaact gaagtagaag
    31081 ataaacaatt cctggtttta tgtgaaagat tggtcagaca aacatttgct attgttgatt
    31141 ttactgaagt agacgaggaa aatgcccgct ttgaattatt tgctcgctat gcatctattt
    31201 tacggacgct gatcaagcag ttagaattac gtctgcaaat tgcttatgat cttccttatg
    31261 aaaggctaat ggcggatatc gtgaaagaaa tcatccagga acaattgctg aatacgatca
    31321 aatatgattt agtcgaatac gagaaagaaa agcagtatga tatcattttg actagccaat
    31381 tgaaagagta cccgtcacag aagtccgcta aagtttttgt attcactagc aacgaatata
    31441 attatgattt cccctatttg aaccaatttt taaaagaatg ttatttagaa aaattgaatt
    31501 taaggatgaa taaaacatga aaatcgtgat cattggtgct tcccatgcag gcatcacagc
    31561 tgccctaaat ctaagaaaac ttcaaccgaa agcagaagtt ttactgatcg atgaagatca
    31621 taaagatgga ctaggctatg tttcaaatgg aatcaacctt tatttaaaag gaaagatacg
    31681 tagtttagct gaagttgccc ataacatgcg gactcttcaa gaaagtgggg caaaactgat
    31741 caccgaatgg agagtaactg aacttgatcc agacaaacat cagctcatcc tcgcatccaa
    31801 agaaggaaaa aacgagacca taacctatga taaattaatc gtagcgacag gttcctctcc
    31861 tgtcaccttg tataaacaaa ttgaagctga aaatgtctac acctataaaa atctagtgca
    31921 atctaagcaa gtacttgccg ctttaaaaga agcaaaagaa gtagtgatct tcggtgctgg
    31981 ttacattgga ttggaattag cagatgcttt gagaaataaa ggatacatta ttcatctagt
    32041 tgattatatg ccgaatgttt tatcaagata tttcgataag gatatgatca acagttttca
    32101 aaatcagctg caaactaagc aaataaattt ttatccaaat gaattcttga tcgattggaa
    32161 aaaatcagaa gagaaagtag tatccgtaca acttctgtct caaacaataa aggcagatat
    32221 ggtcattttt tctgcccaaa ctcgtcctaa tacgacattg ttaaaagaaa aagtggcttt
    32281 gtatgaggat gagactgtga tagtcaatga atatctgcag acaagtgatc cggacatcta
    32341 tgcgattgga gatatcgtcc cagtcagttt tgacaaaaat aaacggcatc tctttttacc
    32401 tttagttact cgagctgtac atatggcaag agcagtagcg ctaactttga gtggtcagcc
    32461 aaccgcttac gacctcagac aaaagataac agcagcagtc attatagact actttttagg
    32521 aactgtaggg ctgacagaag atgaagcgcc attcttggaa caaaataccg gttcgtgttc
    32581 aggagaattt gatctatttc cacaatacga cgaagaaaac aaaacagtaa atgcaaagct
    32641 gatctaccat ccggatacat tagaaataat tggcggacag ctgataagcc aagaattctt
    32701 attatcagat ttgaacctgc ttgcggatat cgtgaaacac aaaactacga ttccacagct
    32761 agcagttgag gattttggat ttctgtctga atacacctct cgttttcact acctcaatga
    32821 gttagctttt aaagtactag ctaagagagc aaatagcaat agagtagata aaagactgtg
    32881 agaaatatca cggtctttta ttgtgtgggg atataataaa tattaatcaa tcgagaataa
    32941 aaaattcata taatgaaata gcggaagaat tttttgagca taatattcgc caaaaattta
    33001 tcaagtgata aaattgttgt ataaaatgat tatttcagga ggaggagtta aatgttttta
    33061 gcattaaatg aaattatgca ttcaaagtta cgttatgcgt tagttgcagg ggtgatgttt
    33121 ttgatcgcct acctggtatt ttttttgact gggttagctt atggcttagc tcaagataat
    33181 cgaacagcag tcgacaagtg ggaggcagat tcgattgtgc tttcgaaaga tgcaaattca
    33241 aatctaggga tgtccatgat tactaagaag atagcagaag aagtcgaagg tgggaaagtg
    33301 gcgtatcttg cccaaacacc tggagtcgtg actagtaaag acagtacaga agaaggaaaa
    33361 atcaatgtta gcttcttcgg tatcgataaa aatcaattca tcatgcccaa tcttgttgaa
    33421 ggaaaagctt ttgataacga tgatgaggca gtaggcgata ttagtttgaa agaagaatat
    33481 ggtctagcca taggagatac agttaagtta tctggtagtg acaagacgtt taaactaact
    33541 ggctttacgg atcatgccaa attcaatgtc tcaccagttc tctatacaac catcaatgct
    33601 taccaagaaa tccgcttcga aaaagaagat acaagtgaaa atgcacggat taacgcaata
    33661 gttgtcagag gtaaaatcag taatttacct gaagatttag aacagatcaa aatttctaaa
    33721 ttcatcaatg aattacctgg atataatgcg caagtattga cttttggatt tatgatcgga
    33781 tttttgatcg ttattgcggc tattgtgatc gggattttca tttatgtact gacgatgcaa
    33841 aagattaata tctttggtgt aatgaaagca caagggatca caggaggatt tattgcgcgt
    33901 tctgtagtgg cacaaacatt cattttatca tttgttggta ttcttcttgg tctattagga
    33961 acagtgggaa catcccttgt tctacctgat gcggttccat tccaaagcaa ttggctgttt
    34021 tttggcgtca ttagcttgtt gatgttagta gtagctgtgt taggcgcact attttctgtt
    34081 cggacaatcg taaaaatcga tccgttgaag gcaattggat aagaaggagg aagaacatga
    34141 aagcaataga atttaaagaa gtagaaaaaa gtttccaaga tggcgaccaa gtagttaaag
    34201 cattgaaaga aacaaccatg agtatcgata aaggggaatt tgttgcaatc atcggtcctt
    34261 ctggttcagg taaaagtact tttttgactt tggcaggcgg attgcagacg cctagcagtg
    34321 gctctgtatt tatcaatgat caagaattca gtaatcaaaa agaaagcgaa cgcgttaaac
    34381 tacgttttaa agaaattggc tttattttgc aagcttccaa tctggttcct tttctgacag
    34441 taaaaaagca gttattatta gtagataaga ttaaacatga acatcgtcaa aaagaagcaa
    34501 acgaattatt tgagcagctt ggcgtggata agttgatcaa caagtatcca gaagaactat
    34561 ctggtggaga acggcaaagg gtcgcaatcg ctcgtgcatt gtacaatgat ccgtccatta
    34621 ttctagcaga tgaaccaact gctagtttgg attcagaaaa ggcacgagaa gtagtcgata
    34681 tcttggcaaa agaatcaaaa gacaaaaata aagcaacgat catggttact cacgatacac
    34741 ggctgattga caaatgcgat agaatattta tgatggaaga tggtgtattg aaagaaaaag
    34801 aataaccaaa aagcaggtgg aaaaatggtc catcgataca acaaagatac acagcaaaaa
    34861 attttgaaag aagcgactga cttgtttatg tccaagggat atctcggcac ttcaacaaga
    34921 gaaatcgcac aaaaagttgg gatcacccag cccaatctct atcattattt cggtgataaa
    34981 gaaaaactat atacggcagt tttagaaaac cacttaaaag atgttggaaa agctttgaga
    35041 gaaatcgttc aaacaagcga aacgggattt caagtaacct tgacaaaaat ggcgcagttt
    35101 ttgattgaga cacatctggt cgatttgttt ctgatgcttc acgatcttga aagcaatctt
    35161 tcaaaggaaa caagagatca tcttttcttt ctatggaaga aaaattatcg tgaacctttt
    35221 gaagaaatct tttcagcaaa ccaatctgtg ctaagaaatg ggatttcaaa agaaatcgca
    35281 gcaaggcatt ttttcttagt gttagcacct tacatcactc ggtcgaaaag acctgtggaa
    35341 gaaccgctaa atgtggcgca attgatcgat ttatatttga atggtgtatt aggagaagcg
    35401 taaagctgtt atatagggag aaaatagtta ggtgaagaag caagatatga cgaagatcgt
    35461 tatatcttgc ttctttatat aggtagtttt tcataaagac aaatttgtta ttctctcatg
    35521 attatcaggg tatattttta tttattatgt cggtgttcca ggaagtgttt taatttaata
    35581 acgaagaaat taattgtttg tagaatgaag gaaactattt attgggtaga tgaatcacca
    35641 cgaatagtca aaaggagatt tcaggatatg ccaaaaatga aaaaaatcat tttagcagta
    35701 atgttatttt ctacggttat atcttttaca gccccttttt tgcttactta agctgatttt
    35761 aagttttggc ccttagtctg gatctacgag gtgagagtca agaatgaaaa aaataattgg
    35821 agcttttgta tttgcaatca ttatcattat tatctgtttt caaatagatg tttttggtca
    35881 aaaaaagata gttggacaaa cggtcagcag tacgaacagt acggttacaa agacaaagga
    35941 atcatttgat gatacagaac aagagacatt aatcaaaatc ggaaaaaatg accgtacact
    36001 atatacagat gactcgctaa ctaccccact tgcaactata aacggcggag agctggcgga
    36061 gtttctttct gaaacaaaaa atgcttatca gatcaagaca aatgatggct atactggata
    36121 tttagccctc gaagatggaa ccaaagtcac aaagaatata caaacaaaac cggaagagct
    36181 atctgatgca gtgattgtat tagatcctgg gcatggcgga aatgatactg gggcattaag
    36241 caatgatgaa caaacagaag aaaaagatat aacattaagt acagccataa aagtaaaaaa
    36301 ggcgctagag gatgctggag caacggttta tttgacccat actacagatg aattagtaca
    36361 gcttggagat atatgcgact acagtgaaga aaaaaaggca gatgtcttta tcagtttaca
    36421 cgcagactct acggaatatg cgaatgaagc aactggaatc acgacatatt attactatgg
    36481 acaagaggaa acattagcac agacaattgc tgactccttc actgatctgc ctctggattc
    36541 aagaggaatc tccactggga actatcaagt tcttcgagaa aatcttcaac cttctatttt
    36601 aatcgagatg ggttatatga ataatgatag tgatttaaag gaattagtaa cagacagtta
    36661 tcagcagcag attgctgcaa gtttgacaca agcattgacc acttattttc agtaataaat
    36721 aagcccagcc aaacaaaaat agctcctcat atttttcgtt tggccgggct tattatcgca
    36781 gatgcaagct tttgaacacc gtttattttt ttagtagctt caaagctttt tctgtatttg
    36841 caaagtgaag tttggcatat ttttcaagca taggcatgcc gcctttctcc aatagttttg
    36901 ccgctacttg gtcagatttc gttccagctc ctgatggaag ggtgattcca gcttcagctg
    36961 atgctttgtg taactcttcc aaaaagctgg cacgactgat gatagaagca gcagcaacag
    37021 ctaaatgata ttgttctcct ttagtaacga agtacagttt ttcttgtaat tgattttttt
    37081 ctaaacgcgc atatttttta aaatttgctt ccggcgtaaa ctgatcgatc aaaatcgctt
    37141 ctggtttttc tggctcgatt ttttttagca gcaaatggat cgcttgattg tgcaaagcga
    37201 ctttcatccg aacggcattg tattttggtt ggatctcatt gtatttttct ggagataaga
    37261 caagcagttt ataggggatg gtttttttta agatgtcagc aagctggatg atttggctat
    37321 cttttaactc ttttgaatct ctaacaccaa gtttacgtaa ttctgcaagc tgatcacgtt
    37381 tcacataagc agcacatact gttagcggac cgaaatagct gccatttccg acctcatcac
    37441 tacctaaaac ggataattga ctgatatttt ttggcaaaga agaggctgtt tttgctggct
    37501 tggcttgttt atcctgtgcc tccccccatt tatcagcttc tattgctgca ttcgtacctt
    37561 gaaacatcac ttttccggat gtataagcgg taatggtcgt cccgtctttt tttgcagcaa
    37621 agctagtata aggaatctgt ttatttaaca aataagagcg atagtatgct ttcatttgac
    37681 taagttggtc gtttgttgct ttgataatga cgttgctcat aaaattctcc tcgtttttat
    37741 acataaaact ataccgcaac tcacgacttt tcgttttcta agagattgcg agacatagca
    37801 tttttttaaa gtttatcata taatagttaa gtatgtgaaa aatatttgtg gagaacggtg
    37861 aaagaaccaa cttcatggta cactaaataa aactttcatc aaataggagg aaatgattcg
    37921 tatggcgcac gaaaaaacac gctacaaagc ggtgattgcc aaccaaacat atactattat
    37981 cggacgggaa accaagcatc atatggatat cgtaacgaag ctgatcaatg aacagttagc
    38041 agaattgaaa caattatcac cacaaatgga taatgaacaa gcagcaatct tgatggctgt
    38101 caatgcgctt tcggatcaat tgaagaaaca agaaagaatc ttggagctgg aagaagaaac
    38161 agcagaatta aagaaaaaaa tgatcaaatt tacagagctt gaaaatcgtg tgaaacgcat
    38221 tgaagcaatc gagaacgaag ctcgggaagt attgaaagaa aatggtcaag cagatcatac
    38281 cattaaaaat catgtggaag cacaacagat tttaaacgag aaacgaaaag gacaaattaa
    38341 acaaaaatct tctaattaag atccttttat caaaataaga aaaatttaaa aaaagttaga
    38401 agagaaagga acgtagcatg ctgagtttgc tgattttatt tttattgcta attgcgtttt
    38461 tttctggagc acgtcgcggg tttgctttgc aaatcatatt cgcgattgga tatgtattgt
    38521 catttattgc tgcccagcat ttttacaaac cattagcaag tcatttggaa ttatacattc
    38581 catacccagc tgtcacgcca acatcaaagt tagcattttt tgatcaggta tttgcctttc
    38641 acctagatga agctttttat gcaggaactg cttttctgat tattttgcta attggctggc
    38701 tgcttacccg atttgtgggg gtttttgtcc atggattgac gtatgtgccg atcttgagac
    38761 aggtagactg gattgcagga ggcatcctgt cactaatcat ggcttacgtt actatatttt
    38821 tgattttgca gttacttgcc tttgttccat tagatgtggt ccaaaatcaa ttcggcgagc
    38881 atagtttcgc tcgctttatc gttgagagaa cgccatttct taccaacaag atgtatgagc
    38941 tttgggtaac gaatattttg aattaaggaa aatagtgctg gaaaatattt cgttttttgt
    39001 acagagtaga ttatttaaga aggctgcgta gcgtatgtcc ggcacagctc ttttttcata
    39061 gtgaaagata aggtgataaa atgaataacc gaatccttga aacattggaa tttgaaaaag
    39121 tcaaacagat ggtcagacaa tttgtagtga ctgcacaagg aaaagaagaa ctggcagaat
    39181 tggtaccagt gagcgaggaa caaacgatca caaactggct tcaagaaact gaggatggat
    39241 tgaaagtgca acgtctgcga ggcgggatcc ctatacctaa acttgaaaat atccgtcccc
    39301 atatgaaacg gattgagatc ggagcagact tgaatggagt ggagcttgca caagtttcca
    39361 gagtattgtc tacgacaagc gaattgaatc gtttcattga tgatctttat gacagtgaaa
    39421 tcgaatttgc acgtttgtat atgtgggcag atcagttagt cacgatccct gttttgagcc
    39481 gccgtttaaa ggaagcaatc gatgaagacg gacgcgtgac tgatgacgct tctcctgagt
    39541 taaagagcat aagacagaac attcggcgaa gtgaacaagc tgttcgtgaa cagttagatg
    39601 ggatcgtccg tgggaaaaat gcaaagtatt taagtgatgc gatcatcacc atgcgaaatg
    39661 accgctacgt tatcccagtc aaacaagaat accgcggtgt atttggcggt gtagtccacg
    39721 atcaaagtgc ttcaggacag actttattta ttgaaccaaa acaagtggtg gatttgaata
    39781 atcgtttgcg tcaataccaa atcgctgaac gaaatgagat tcaacgtatt ttaagtgaac
    39841 tttctgctga acttgttccc catcgtcaag aaatcatcca caatgcttat gtgattggta
    39901 aaatggatct gatgaatgca aaagcacgat ttggtaaaga agtaaaagct atcgtgccgg
    39961 gaatcagtga agacaatcat gtcgtcttga aacaagcaag acatccactg atcgatcagg
    40021 aaaaagtagt ttctaatgac atcacaatcg gaaaagacta tcaagccatc gtcattacag
    40081 gacctaacac aggtgggaaa acgatcacgt tgaagacttt aggtctgtta caactgatgg
    40141 ggcaagcagg attaccgatt cctgcaggtg aagaaagtca aatcggtatt tttgaggaag
    40201 tatttgcgga tataggagac gaacagtcga ttgagcaaag tctatcgacc ttttcttctc
    40261 atatgacgaa cactgtagat attttatcaa aagtcaatga aaaaagtctg gtattatttg
    40321 acgagctcgg tgcaggaaca gatccgcaag aaggtgctgc tctagcaatc gcaatcttag
    40381 atgatcttgg taaaaagtct gcttatgtga tggcaacaac gcattatcct gaattgaaag
    40441 tttatggata taatcgggca aatacgatta atgccagcat ggagtttgat gtggatacat
    40501 taagtccaac gtatcgtttg ctgattggcg ttcctggacg aagcaatgcg tttgaaatct
    40561 ctagccgatt aggtcttgat acagaagtta ttgatgaagc gaagcagttg atgaatgatg
    40621 agagccaaga tttaaacgaa atgatcacag atttagagaa tcgccggaaa atggcagaga
    40681 ccgaatatct ggaaatgcgt cattttgttt ctgaggcaca agaacttcac gatgatttga
    40741 aagaagctta cagctatttc tttgaagaac gtgaaaaaga aatggaaaaa gcaaagaaaa
    40801 aagcaaatga agtcgtgtca gaggcggaag aaaaagcaga aaaaatcatt gctgatattc
    40861 gtaaaatgca acagcagatt gggcaaggaa atgtgaaaga gcatcagttg atcgatgcaa
    40921 agacccaatt agcaaatcta catcaagaag aaactttgaa gaaaaataaa gtattgaaga
    40981 aggcgaaaga acaaaaaacg ttgaaaccgg gagacgaagt gttggttacg acttacggcc
    41041 aaagagggac gcttttacgt aaaaacggga atcagtggca agtagaaatc ggtatattaa
    41101 aaatgaatgt gtccgaagac gagctgacgc ctgttgctcc tcaaaaagag ccgacacaac
    41161 gagttatcca tgctgtacga tctgaatcta gtagtcacgt gccgaatcaa ttggaccttc
    41221 gaggaaaacg atacgaagaa gcgctaagtg aggtagatca atatttggat tctgccattc
    41281 tagctggtta tcctcaagtg actattgtcc acggaaaagg aacaggcgca cttcgcaagg
    41341 gaattactga ttatcttaaa aaccatcgga gcgtcaaaag ctttgagttt gcaccggcaa
    41401 accaaggcgg aaatggtgcg acaattgtta aatttaagta aagcttacta aaggtaagca
    41461 gatggttcgc tatccaagta tagaaatgct atactagtca tagattttaa aaatggaggt
    41521 ctttgatatg tcacaagtaa ttacagataa gacatttaat gaagaaacag ataaaggact
    41581 tgtattgatt gatttttggg caacttggtg tggtccatgt cgtatgcaag caccgatttt
    41641 ggatcagttg gaacaagaat atgatgaaga agaattcaga attgctaaaa tggatgtgga
    41701 tgaaaatcca gaaactcctc aacaattcgg tatcatgagc attccaacat taatgttgaa
    41761 aaaagatggt caagtagtag aaaaagctgt tggtgttcat tcaaaagaac aactacgtca
    41821 aatgatcgat caatatttat aaaaacttac agcaaaaata agctgagggt gggactgaat
    41881 ccagttccgt cctcggtttt tttgcggttg agttaaggtt attcaaatta atattttttg
    41941 agtataatta cgttgtcagg ctggattgtt tttttgacag acatgctata ataactttaa
    42001 ttgaatatga aggggagtac cagatgaacg aacgaatcaa aaacaaatta gctttacttc
    42061 ctgatcagcc cggctgttac ttgatgaaag ataaaaatgg aacgatcatt tatgtcggca
    42121 aagccaaaat attaaaaaat cgtgtccgct catattttac tggtagccat aatacgaaaa
    42181 cggaacggct tgttagtgaa atcgaagact ttgaatacat cgttacagaa tcgaatatcg
    42241 aagcattgct cttagagatc aatctgataa agaaaaatga tcctaaatac aacattatgt
    42301 taaaggatga taaaacctat cctttcctta agataacgaa tgaaaaatat ccccgtttgg
    42361 tgattactcg taaggtacta aaagacaaag cactgtattt tggaccatat cccgatgtcg
    42421 gtgcggcgaa tgagacaaag aagatcctcg atcgattatt tcctctgaga aaatgcaaac
    42481 ctagtcagac gaaggagcct tgtctttact atcatttggg acaatgtctg tgtccttatt
    42541 atttcgacgt agatccgaaa gtctatacga aaatcgttga tgaagtgaaa aactttttaa
    42601 acggaggaca cgaaaaaatc gagtcagaga tccatcagaa aatggaaaaa gctgctgaaa
    42661 acatggagtt tgaaaaagct gcagaatacc gagaccagat ccgtgccatt gagacaatca
    42721 tgactcgcca aaaaatgaca aatactgatt tactggatcg agatgtattt ggttatgcga
    42781 ttgataaagg atggatgtgt gtccaagtgt ttttcgtaag acaaggaaag ctaattgaac
    42841 gagatgtttc aatgtttcct ttctataacg aagcagaaga agatttcttg acttacctcg
    42901 gtcagtttta tcaggaaaat gagcatttca ttcctaaaga agtcttgatt cctgatacga
    42961 tcgacaagcc cagtgtagaa gctttgttag ctacgaaagt gctgcaacca aaacgaggag
    43021 aaaagaaaaa attagttgaa ttggcgagta agaatgcctc agttgctttg aatgaacggt
    43081 ttgatctgat tgccagaaaa caggaacgga cgatcggagc agttgaacgt ttaggaaacg
    43141 cgatgaatat cccgacccca atacgtatcg agtcttttga taactctaat atcatgggga
    43201 cgaatcctgt ggctgcgatg gtcgtattca ttgatggaaa accggcaaga aaagaatatc
    43261 ggaaatataa aataaaaaca gtcaacggac cggatgatta tgcatcaatg agagaagtca
    43321 tctatcgcag atattcgcga gtgattcgcg aagggttgcc tttacctgat ttgatcttga
    43381 tcgatggagg aaaaggacaa gtagatgtgg caaaagatgt cttagaaaat caattaggaa
    43441 tcgatattcc tattgctggc atggcaaaaa acgataaaca taaaacaaat gagttgctat
    43501 tcggtacaga tttggctgta gtaccgcttg agcgaaattc accagagttt ttcttactcc
    43561 agcgaatcca agatgaagtc catcgattcg caatcacttt ccatcggcag ctacgcagta
    43621 aaaacagttt tgcttcccga ttagatgaaa ttgaagggtt aggaccaaaa cgaaaaaaaa
    43681 tgctgcttaa agagttcaaa tcgttgaaaa atatcacagc agccacgaca gaagaattac
    43741 aagaaatcgg actgccgaaa aatgtcgcac aaaacgtatt tgataaatta catcaggaat
    43801 gaggaaaggg cactttacaa agaaagtaaa gtgtcctatc attttatgta caacaaaaag
    43861 agcgtttacg ttttgttggt aaataataaa tcgcttacgt ctggtgtcta aggatatatg
    43921 tgatttcttg aagaaggagt gaagaaatgt tagaagtcaa ggatcttgtt aaaacttttg
    43981 ggaactacac agctgtcgat catgtctctt tccaaatccc cgatggaaaa atcatggggc
    44041 taattggcca aaacggggct gggaagacaa cgacttttcg tttgatcttg gattttttga
    44101 cacaagacca aggagaagtg ttatggaatg gccatcatct tggggagaaa gattatgatg
    44161 tcatcggtta tcttccggag gaacggggac tttatccgaa agtaacgatt caagaccaac
    44221 tgctttattt tgcagaactt agagggaaat caagaaaaga gattgaacca aaaatcgact
    44281 tttggatgga aaaatttcag gtaaaaggga aaaagacgga taaagtaaaa tctttgtcta
    44341 aaggaaatca gcaaaaagtc cagctgatcg ccacgttaat ccatgaacct aaattgatca
    44401 ttttggatga gccattcagc gggttagatc cggtaaatgc tgaattgcta aaagatggaa
    44461 tattggaatt gaaaaaacat ggttcatgtg tcattttttc tagccacaat atggataatg
    44521 tagaaaaaat ctgcgatcat ttgatcatgt tgcgaaacgg aaaaatggtt ttgaatggaa
    44581 aagtacatga gattcgtgaa tcatttggcc gtacgaagtt attcttggaa tctggtttaa
    44641 gccaacagga agtagaagaa atcgaaggcg tgaaaaaagt gatccttcat gaagacaata
    44701 cgttagagat taccttagaa gatcctgaag ctgggaaaga aatctttact cgtgcgactc
    44761 agttcggcta cattcctatg tttaaccagc aaccgccaac tttggaagag attttcaaat
    44821 tgaaagcagg tgaaatcgat gcgtaagttt tggattatta ccaaagatgt ctatttaaaa
    44881 aatgttaagt cgatttcttt cttgatcatg atattagtcc cttttatcat gctgggtatc
    44941 atttatgtag caggaaactt tgctcaacaa aatagtgatg tcaataaaat cggcgtattg
    45001 gctgataatg agcaaatcag tcagcaaatg agccaagtca aaacagatga ttttgagttc
    45061 aaagcagttg attctgaaga aaaagcacaa aaagagcttt cggaagaaaa aatcgatgcg
    45121 tatttagtag tctcaactga gaatgataag ataaatggga tgctttatag cgaaaattca
    45181 ttaggacaaa gtacacaact actgatacaa caacaactga ctggcttgca atcgatgctt
    45241 cgagcgagtc aattaggcat ttcaccagaa gaagtagcta gcttgagcca acaagcaagt
    45301 ttgactcgac aaaaagtcaa ttttgattca aaaggaaaaa tgacagtcgg agaagataac
    45361 tcgacagtcc aatacgtcgt aagttatgta gcaacgattg tcttgttcat tattattttg
    45421 acgtatgcgc agatcattgc acaagagatc gcttctgaaa aaggcacgcg gatcatggaa
    45481 gtgatcctgt ccagtacgac tgcacaaaag catttttacg gaaaattaac aggtgtttta
    45541 cttgttgcat taacgcaaat ggcattatac ggtattattt ttggtatagg atacagtcaa
    45601 ttcaaagata tggaaatggt caaaaatata ttagacggta tctcattgga taacttgatt
    45661 ggaccattct tgtggtattc tcttctattc atgatctttg gcattttgat tttttctgtt
    45721 ttagcagctt tatgtggctc tttggtcaac aaagcagaag atacagcaaa agcaattttg
    45781 ccagtgactt atctatctct aggcggatac atgcttggat tgatactagg agcttcagat
    45841 ccaaacaata tcgttattcg gatcacttcg tatattccat tcttatcttc atatattatg
    45901 ccggttcgct tagcaaatga gacagtcggt atgggtggag cattgatttc cttgttggta
    45961 cttgtagccg taacattctt attgatgatc ttgtctgcaa acatgtacaa atcgaatgta
    46021 ctcgtttata gcgaaggcgg attatggagt tcattgaaac aatcgatttc aattatgagg
    46081 aatgaacgaa aaaaagcata aaacaatcct aaaatacaga aggaagctgg acatctattg
    46141 ttcccagctt ctttttgtat agacctattt tatttatttt actatattgg atatatattc
    46201 attgaaaata gtgtataaca actttataat attgagtata ccaatttttg gaggcgtttt
    46261 aatggaccgt tatttagatt tactttcaga aaaattcccc tcaacggagg atgtcatcac
    46321 ggaaatcatc aatctcgaag caattttgca gcttccaaaa ggaacagagt tatttttgag
    46381 tgatatacat ggcgaatttc ctgcttttga tcatattctg cgtatcggtt caggaaatct
    46441 taaagaaaag atcaaagacc ttttttctga acaaatgacg gaaacggata tcagccaatt
    46501 cactattttt acggcttatc cagaatatgc attaacgact gattggtaca aagaacaaga
    46561 taaatcgcag ctgatccatc atttgatcga tcttttacgg tttacgacag tcaaatatac
    46621 ccggtcaaaa gttcgaaaag gattgccgaa agaatatagc tatatcattg aggaactctt
    46681 gtatatcgat gaccgtatca atggaaaaaa agactatgta aaaaaaatca ttgaacaact
    46741 tgttttgatg aaggaagaag aaaaattttt aacgaaactt gcacagacga tccaaaaatc
    46801 tgtcatcgat catttgcata tcgttggaga tatttttgac cgaggaactc aagcagccaa
    46861 agtgatggat cgaatcatgg atttttcttc tgtagatatc caatggggaa atcatgatat
    46921 tttgtggatt ggtgcgtatt gcggctctga ggcatgttta ctgacacttt tacggattgc
    46981 agcacgctat aattatttat ttgaattaga gaaagaatat gggttgaatt tacgtccatt
    47041 gttttctttt gctcatcaaa cttatcagaa gaatccagta tttgcgccta aaaacagtaa
    47101 aaatctatcc gaaagagagc aagaagaatt agaaaaaatc catcaggcac tttctatcct
    47161 tcaattcaaa tcagaacatc agttattgga tcgtcgacct gaatttaaaa tggacgatcg
    47221 taaattgctg gaaaaaatcg actatgaagc atcaacgatt gatttagaag gtcagctcta
    47281 tgagttgaaa ggaacttgtt ttcaaacgat ccagccagat gatccaaaaa aattcttgga
    47341 agaggaacaa aaagtgattg atagtttgat gtattcattc cagcactctt tgagaatgca
    47401 aaaacatatg acgtttttga tcgaaaaagg gggaatgtat ctcacgaaca atcagcatgt
    47461 gctgtttcat ggctgtatac ctgtagacga gaagggacag ctcttagcat ttgaattaga
    47521 ccaatcttat cgcggaaaag aactgctggg cttttttgaa aaacagataa ctgaaagcgc
    47581 aaaagatctg actgcaaaag aagatttagc aaccgatttg atttggtatg cgtggagtgg
    47641 gccattttcg cctttattcg gcaagagtaa aatggcgact tttgaacgtt attttctcac
    47701 agagcctcat acgcacgtcg agaaaagcaa tccttattat cacttgcgag atgaagagtg
    47761 gttcagtgaa aaaattttag cagaattcga cacaaaagaa gaagggtcag caatcattaa
    47821 tgggcacacg ccagtaaaag taaaaaaagg ggagtcaccg atcaaagcaa atggaaaagt
    47881 atttgtcatt gacggaggtt tatccaaagc ttatcaaaaa acgacaggaa ttgccggtta
    47941 ttcactatta tgcaattctt atggtttcca aatcgtgaca cattatccgt ttttgcctat
    48001 tgaacaacaa tttgcaaaaa aatccgacca aacaaatgtg aaaaaagtaa tcgaacagca
    48061 actaccacgc aaactcaatc gtgatacaac aaaaggtatg gaactgcagc agcagattac
    48121 ccagctcaaa aggctgttgg actatcgaaa ggacgattga gaagaattcc agtgaacatt
    48181 gttccataga ctggagactg agacaaaagt cgtgtcgcag tctctttttc cggataaacg
    48241 gtgttcttct gccaactcct cggtcacaag tcacactatt caacaaacat gaaaagctgt
    48301 tttttgaaat tgctccttgt gactcagagt tgaacggcag gctcacgacc tcttggtata
    48361 aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtgaaaag
    48421 ccattttcac aaaatttttc ttaatactcg aaactaaaca gctttttcac aaccccctca
    48481 gaaagtctat taaaattcat tcacaaagaa atcgaataag cgctgttcag atggatgtac
    48541 acggtgggta agttccggat gccattgaac accgaggatt ttatgcatat catctttgct
    48601 ttgaaccgct tcaatgagtc catcgttaga aaaagcgatg gcttttaata aaggtgaaag
    48661 atctttgact gcctgatgat ggtaagaatt cacaaaatat ggatcgggga gaaggttgct
    48721 taacaaactg tctgaaacaa tcttgacttc gtgagtcgta aattgcgggg cagtgggctg
    48781 ttgttcatgt tttactgacc actttggata taatgaaaga tcttgataaa gtgtaccttc
    48841 caaaacgaca ttcaatagtt ggagtccccg gcagactgca aaaattggtt ttctttgttt
    48901 caatgcttcc aagatcagtg cctgttcaaa ttggtctcgt tgaatatttg tttccgttag
    48961 ctttggatga ggatcttcca tgtaaagttg tggactgaca tcttgaccac ctgcaagaat
    49021 aagtttgtcg atttgtccaa tcagttgagg agctgaatct ggagacatga ctggcagaat
    49081 aagaggcaat ccttgagcat ctaaaactgc atccacgaat ccttgcggtg tatagctcac
    49141 ttgatttcct tggaaagtat ctgttgctcg aatcagttga tttccggcaa tgccgatgat
    49201 tggtttcatt tgtattcccc ctaaagtaga cttattgtaa caaggattta aaaaaaatta
    49261 aactatgtaa gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag
    49321 aaatttcgtg tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa
    49381 agtaagcaga aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga
    49441 gaaggacatt ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt
    49501 caatgaaaaa agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta
    49561 gaagctttgg aacaaatccg aaaagcagaa gaagaaaatg aaaagcgtgc tcaaacactg
    49621 cggcagcagc tgaagatcta tgcaacagaa aaagagcagg aattggaaac gatccaaaca
    49681 aactatcgac aacaactaaa acagttggtg gaagaaaaag aacaatttga aatacaagaa
    49741 ttagaaaaag aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa
    49801 gatcaatatc aaaagcatca gagtcaagcg atcggtgcga tcattgaaag ggtgaaagaa
    49861 acttatggcc gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga
    49921 gaggatttta caagcagtac aaggaatcca aaacgttgaa atccgggatc tgtatcaaaa
    49981 tagaacaaac aaccagtggg tggagaagta ttttccagat accacgatta ttgataaaga
    50041 agcaaaatta agcgctttag gtaatcggtt gaaagacatc caagaagcta tccaattcat
    50101 acaacaccac ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt
    50161 acaagatttg gaagccaatt attccgaaga aaatttcata aataaattga aagaagtcct
    50221 tgatttaaaa gaaaagtggg aacaactaac tgaagaacgt gatcgtttag aagaagagga
    50281 agaatggctt cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac
    50341 gaccattttt gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact
    50401 gagtaagctt gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct
    50461 ttcgtatgtt gctttaaaaa aatcagttgc tgttatagaa gaaattgctg ggaaatacgg
    50521 gttcaataaa gaaacgtatc cttatgaaca aaatcctaaa gaaaaactgc aagagaccaa
    50581 gcagcaatta tccattgttc acgagcagca aaagaagata acgacagcgt taggtcagtg
    50641 cagtggctat atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg
    50701 agaaaaaata aaagaacggt ttatccatgc gacttatctg atcgttttgc aaggttgggt
    50761 agatgcagaa gaaaaacaga atttgctgca tgtgttaaac gaaaccgtat cagaagaaga
    50821 tatatacgtc agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa
    50881 attgaaaaac catccgcttg tcgaaccttt tgaattgctg actgaaatgt acagtctgcc
    50941 aaaatatgaa gaagtagatc caacaccttg gatgacacca ttttatcttg tcttcttcgg
    51001 gatgatggtc gcagatgttg gttatggttt gctgatgctc attgggacaa tactagctca
    51061 aaaactactt gttctaccac gcgggatgaa acgtttcgta aaattctttg agattctctc
    51121 gataccatcg atcatctggg gattgatcta cagttcattt ttcggacagt cattaccaaa
    51181 agaactgttt ggcatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac
    51241 gattttgatt ttatccgtga ttttcggtct gatacagatt ttggttggat tatttgtggc
    51301 agcaaaggaa aatatcagga gaaaagatta cttgagtgct gtcagtgatg gatttgcctg
    51361 gcaagggatc ctaattggga tcgttattgc gttggttggt gccatgttga taaaaaatac
    51421 agcatttgtt taccttggcg ctgggctatc gatcattgct gccttatgta tcttgatcgt
    51481 tccgattatc caaacgccgt ctaaagtcaa aggtgctgcc aaaggtgcgt ataatcttta
    51541 cggactgacc ggctatatcg gtgatttagt cagttataca cgtttgatgg cattgggtat
    51601 ttcaggcgga agtattggag cggcattcaa tatgctagtt gctttcatgc cgccagctgc
    51661 tcgattcagt gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac
    51721 actgctaagt gcctatgtcc atggcgcacg attgcagtat gtcgaatttt ttgggaagtt
    51781 ttatacagga ggcggacgtg cctttgatcc gctgaaaacg gcggaaaaat acgtcaacat
    51841 caatcacaag aaaaaaaaca ataaataatt ggaggatttt tatataatga tggattactt
    51901 aattagtcaa aatggtggaa tggtatttgc ggtattagcg atggcaacag cgacgatttt
    51961 ttcagggatc ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcattgac
    52021 aacaagtcaa cccgaaaaat tcgggcaggc tctgattctg caattgcttc caggtacgca
    52081 aggtttgtat ggattcgtta ttgccttttt gatttttatc aacttaggaa atgacatgtc
    52141 agttgtacaa ggactgaact ttctaggtgc ttctctgccg attgctttta caggcttgtt
    52201 ttcaggtatt gcccaaggaa aagtcgcagc ggcaggtatt caaattttag cgaaaaaacc
    52261 tgaacatgct acaaaaggaa tcatttttgc tgcgatggtt gaaacatacg cgattttagg
    52321 tttcgttatt tccttcctat tagtgttgaa tgcctaagca taaaggagga aaagcagatg
    52381 aacgccattg aaaaaatcat ctctcagatg aacgaagcag cagagcagga aagagcggcg
    52441 ttagaacaag aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaagtt
    52501 gaaacagaac atcaaaaaca aaaagaaaaa caaatcgaat tactcgaaaa aaaataccgt
    52561 cagctaagga atcgccagca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa
    52621 tttttaagac gattgtttgc agatgcggta actgaaatgg aaaattggga tgaatctgaa
    52681 caaatccagt ttatcaaaaa tgcattgtat tcattacctt tgacaggaaa agtagcattc
    52741 attgctggag aaaaatcagc agcctatctt tctcagacgt tgttggatga atggaacaat
    52801 gaacttccct ttatgatggt tttaagtgat gaaactgttg ctgatcaagc gggttttctg
    52861 ataaacgatc aaggagttca gtataatttt cttttcagca gccttgtaca agatatccaa
    52921 ggaacgatga gttttgagat tgccaatcaa ttattcgaat aaggagggat cagtgtatgg
    52981 aataccacga actaaatccc ttgattcgcg gaagagagct tgaacttttg tccaatgaga
    53041 catttgaacg gatgattcaa acaaattctg tcgaagcact tggtgaaata ttgaaatcca
    53101 cgatttacag tccatatatt tatgacggat atgaatatga cttcgaacag aatcttttaa
    53161 aggagcagag ccgtctattt cgctggttaa aggaatcagc accggagcct gaaatcgttt
    53221 ggatctatac gatgcgttat actttccata atctgaaggt tttgacaaaa gctgagatga
    53281 caaatcaaaa tctggaccat ttgtatatcg acgatggatt ttattcatta gaaaccttga
    53341 aggatgccat tcatacgcaa gtttcaaatg aacttcctga aagcattatg gacagtatac
    53401 gagaagtacg tgattatttt gaagagtcta ccattcttca gggaattgac gtgatttatg
    53461 atcgacagtt tttgacagag cagcgacggc ttgccgataa gctaggatat caagaattgc
    53521 tgacagaagt cattgctttt attgacttga ccaatatcac aacgatggct cgaggaatat
    53581 tgcagaaaag aacatatggg ttcatgacga cggtactttc cagctcggga agtatcgaaa
    53641 aagaagtatt tttgaacttt gtagataaag atatggaaga ttacacgaat tttctattag
    53701 agacagatta tgctgatttt ctgcgccctg tcatccgtga gggaaatatt gatctcgtaa
    53761 agcttgaaca gttgaaagat gattatctaa gttcattgta tcaagaagca caaacccagg
    53821 ctttcggacc actgccacta ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc
    53881 ttcgtttact ggttatcggc aaaagaagcc atttcgaatc agaaacaatc agaaagcggg
    53941 tgagacaagt ctatgacgca taagatcggt gtagttggcg acaaagattc tgttttgcca
    54001 ttcaaattgt ttggctttga tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca
    54061 attgaagaaa tggcaaagaa tgagtatggc gtgatttatg tcacagagca atgtgcagca
    54121 ttgagcccag aaacgatcga acgttacaag gaacaaatga tacctgctat cgtattgatt
    54181 cccaatcatc aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa
    54241 gcagtaggtc aaaatatctt atagtatgaa aaggagatag aaattttgca aattggaaaa
    54301 atcataaaag tctccggacc tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa
    54361 gacatgtgtc tagtgggaga tctaggagtt atcggagaaa ttatcgagat gcgtggcgat
    54421 gtggcgtcta ttcaagtata tgaagagact tctggaattg gtccaggcga accggttcgc
    54481 tctactggtg aagctttatc tgttgaatta ggaccaggga tcatttcaca aatgtttgac
    54541 ggaatccaac ggccattaga tacctttatg gaaatcacac aaagtaattt cttagggcgc
    54601 ggagttcaat tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa
    54661 gccggagaaa cggtgagtgc tggagatgtt atcgggatcg tagaagaaac aaaagttata
    54721 aaacataaaa tcatggtgcc aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt
    54781 tcattcacta ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga caaaaatttg
    54841 acgatgcttc aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa attaaacccg
    54901 gatgcaccca tgatcactgg acagcgagtg attgatacat tcttcccagt gacaaaagga
    54961 ggagcagcag ctgtacctgg tccttttggt gcagggaaaa cagtcgttca gcatcaaatt
    55021 gcaaaatggg ccgatgtgga catggttgtc tatgtgggat gtggcgagcg tggaaatgag
    55081 atgacggatg ttttaaatga attccctgaa ttgattgacc ctaatacagg agaatcgttg
    55141 atggaacgta cggtattgat tgctaataca tcaaacatgc cagtagcagc tcgagaagct
    55201 tctatctata cgggaatcac aattgcggaa tatttccgtg acatgggtta tgacgttgct
    55261 atcatggcag actccacttc acgatgggca gaagcacttc gtgagatgag cggacgtttg
    55321 gaagaaatgc ctggtgatga aggttatcca gcttatcttg gttcacggct tgcggaatac
    55381 tatgaacgat ctggaagagt cattgcatta ggatcagaag gtcgcgaagg aagtataacc
    55441 gcaatcagtg ctgtatcccc gtctggaggg gatatttcgg aaccagtcac acaaaataca
    55501 ttgcgggtcg taaaagtatt ctggggtctt gattccagtt tagcccaaaa aagacatttt
    55561 ccttcgatcg attggataca aagctattct ctttattcaa cagaagtagg gaagtatatg
    55621 gatcatatcc ttcaagctga ctgggcatcc atggtcaccg agggcatgcg tattttgcag
    55681 gaagaacagc agctgaatga aatcgtgcgt ttggtaggga ttgattccct ttcagataat
    55741 gaccgtctga cattagaagt ggcaaaatcc attcgtgaag attatttgca gcaaaatgct
    55801 tttgatgatg tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc
    55861 ttgacctttg gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc
    55921 atggacggta ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca
    55981 gaattagaaa aactagatca aattattggt gaaatcaagc aaacgatgca aacgatcgtt
    56041 tcagaaggag gcatgagcaa tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc
    56101 attgatggcg gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg aagtccgtat
    56161 gcaaaatggc gagattcgtc gtggacaagt attggaagtc caagaagata aagcaatggt
    56221 ccaaatattc gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac gatttttagg
    56281 ccatccttta gaactaggtg tatcggaaga catgatcggt cgagtattcg atggtttggg
    56341 acgaccgaaa gataacggac cagagatcct gcctgaaaag catttggata ttaacggaga
    56401 agtaatcaat ccagtagctc gggattatcc agatgagttt atccaaacag gtatctctgc
    56461 aatcgatcat ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg
    56521 tcttcctcat aaagaattag ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc
    56581 agatgatttc gccgtagtct ttgctgctat tggaatcact tttgaagaag ctgaattttt
    56641 catggaagat tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta tgaatttagc
    56701 aaacgaccct gcaatcgaac gtatcgctac tcctcgtatg gctttgactg ctgcagaata
    56761 tcttgcttat gaaaagggaa tgcatgtttt ggttatcatg acagacatga ccaactatgc
    56821 agaggcattg cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc
    56881 tgggtatctt tacacgaact tagctacttt attcgaacga gcaggaagaa tccgcggact
    56941 aaaaggatcg gttactcaaa ttccgattct aacaatgcca gaagacgata aaactcatcc
    57001 aatccctgac ttgactggtt atattacaga aggacagatc atcttgacta gagagcttta
    57061 caagagtggg attcagccac caattgatgt actcccgtct ctttcgcggt taaaagataa
    57121 gggaactgga cccggaaaga caagagaaga ccatgcagct acgatgaatc agctttttgc
    57181 agcctatgca caaggaaaac aggcaaaaga attagccgta gttttaggtg aatctgcttt
    57241 atctgatgta gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa
    57301 tcaaggattc tacacgaacc gttcgattac tgaaacactt gatctagggt gggaactgct
    57361 atcgatgttg ccaagaaccg aattaaaacg aatcaaagac gacatgctgg acaaatatct
    57421 gccagaaggg aagtgagttc tgatggcacg attgaatgtg aatccgaccc gcatggaatt
    57481 gactcgattg aaaaaacagt tgacaactgc cacaagaggg cataaattat taaaagataa
    57541 gcaagatgaa ctgatgcgtc aattcatcct gttgattcga aagaacaacc agctgcgtca
    57601 agaaatggaa gagtctatga cagaagcaat gtctgatttt gtattggcaa atgcttcgat
    57661 cacggaacct ttcatcgaag aattatttgt tttgccggcg gaaaacgttg atttgtctat
    57721 cgtagacaaa aatatcatga gtgtaaaagt acctgtgatg aattttgaat atgatgaaca
    57781 gctgacagac acacctttgg aatacgggta tttgaattcc agtgccgaac tggatcggtc
    57841 aatcgatcgg tttactaccc tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac
    57901 ttgtcaactg atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata
    57961 tatgacgatc cctcaattag aagaaacgat ctattatatt cgtatgaagc tggaagaaaa
    58021 cgagcgcgca gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca ctgactaagt
    58081 ggtatctttt tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt
    58141 ttgccggctg ttcagcttct cgcattagga ttttttctcc tgattttagt tggcggcggc
    58201 ctacttaccc taccattttt tagtaatagt gggcatggga caaattttat cgatgcgctt
    58261 ttcactgcaa cttctgctgt ctgtgtaacg ggattgacaa cattgaatac agcagagcat
    58321 tggaacagtg ctggtcaatt tttgatcatg cttttgattg agatcggcgg tcttggtttt
    58381 atgatggttc cgattctatg ctttgcattg ataaagaaga aagtcagtct cagcacacgt
    58441 attatattaa aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt
    58501 tacatcctaa aattggcctt tgtgatccaa ttgattggta cggttgcttt atcttttgta
    58561 tttgtgcctg aatttggctg gttgaaaggt atctggtaca gtttgttcca tgcaatctct
    58621 agtttttgta atgcagggtt tgatttgtta ggagacagtt tagccaatca ccagcaaaat
    58681 gtttatttga tcatggtggt atctgctttg atcatttctg gtgggttagg cttcatcgta
    58741 tggcgtgatt tactggaata tcatcagaaa aagaagatga cgattcattc aaaaatcgca
    58801 ttaaccgtta caggtaccct tttattaggc ggattcgttg tgttcttttt cacagagcgc
    58861 aatggggcaa cgctagtgga agggaatatg tttcaacgat tggcaaacac cttttttatg
    58921 agtgtgacac ctagaacggc tggatattat tcagttgatt acctccatat gagccatgca
    58981 ggtctgattt taacgatgtt tttgatgtac atcggcggaa catctgggtc gacggccggc
    59041 ggattgaaaa caactacgtt aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga
    59101 cgtacgagag cggaagcatt tggccgtacc atccgacaag gcgcagtgtt gcgggcactt
    59161 actttatttt ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt atcggtgact
    59221 gaaacgatac ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt
    59281 ggtacagtag gtcttacgat gggattgacg ccagacttga ctgcaatagg gaaattgatc
    59341 attatagcgc ttatgtacat tggacgagtt ggtgtattga ctgtggtctt ctctctgatg
    59401 acaaaagcac aaagaacaca atcaaaatac aaatatccag aagaaagtgt catgattgga
    59461 taatttcatc ttctgacaga aacctagtga tttagtaaaa taagaaaata aaataatttt
    59521 tcttgcttct tggatacttt aagtgcaaaa tgttcgttta ttttttgagg cactacccgt
    59581 tgttgaacct cgggtagtgc ctctttttta ttgaaaggaa tcaataagat ttgattcctt
    59641 tatttatcaa taggttataa ttcatatttt tttatttttt cttttacgat acttagtcca
    59701 gaagctaaaa tttcaaggtt attagaggat agggaaattc gaacgaatgc atctggttgc
    59761 gtacgtgtag taaagcgtct ggaatgataa agtcggatac cgttagcaag aaagtcattc
    59821 tcaattatct tagggtcaat ctcaggatca actggaagaa ttcgatagta agggacacga
    59881 ttcgttggtt tttctacttt gaaaagagaa tcaaaaagct gattggctct ttttgtaaga
    59941 cttaatttag tttctaagat cctatttgct tcatttgaat ggaggatttg agtgatgact
    60001 tccacatcta atccagaagt tttcacattt atattaaaaa ttgcttgttc tatcttgctt
    60061 tggacatgta ctggaactac aagataagca actcgcaatc cagaacaaat aaattttgtc
    60121 attccagcta agtagattgt ttggttaggt aataaatttt ggaaaggagg aatgatcgta
    60181 tgttgatagt aagttgttaa aaatgaatgg atatcatctt caataaccca tagaccttct
    60241 ttttcaatca cttcggctaa ttgctctcgt cttttgtgag agatttgaaa ccctatagga
    60301 ttattacaag aaggcataag aaagacacca tgaatcttct tttttctaca ttcctgaatc
    60361 aatatatcag gtcgcattcc ttgctcgtca aaatcaattg gaacgatctc taaatgatat
    60421 aactgagcca attcaatgaa attagcgtat gtatatcggt ctgttgctat tctatcacct
    60481 ggagagaaaa gtgcagaaag tgtgatagcc aatccatttt gaacaccgga aacaatagat
    60541 agagattcta aggacggaat gactccttga gtttttaacc aatttgctgc agtagtcaat
    60601 tgaaaatttg tgcctttggg atcttggtaa ttaagcaaac caacaagatt tgggttatta
    60661 ctagttgatt gaataaacgg gacaacgaat ttattacatt cttcaaaaga actaaccaga
    60721 ccaaaatcaa tgatatgttc ggttacttca ttggtagaga tggtgatgga ctcaaagaca
    60781 ttgggagaaa taaatgtgcc acttccaata ttcgtataca gcaaaccttt ctcaattcca
    60841 tatttataag ccttgccaac ggtagtaaaa tttaaatcta aataatcagc tagctctctt
    60901 tgggagggta actgagtgtt tttctgtaat ttattcttta aaatatcttg ttctaattgt
    60961 tgaatcaacg atcgataata aggacgcttc aatttttgtt tgtctggtcg ccaagttaac
    61021 gggtaattgt cataagaatt aataggcatt ttctccactc cttaaaattg tataccatac
    61081 aattttacc
//
LOCUS       DAYYKN010000002        58727 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000002 DAYYKN010000000
VERSION     DAYYKN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.002"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..292
                     /locus_tag="ACHBB7_000062"
     CDS             <1..292
                     /locus_tag="ACHBB7_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208051.1"
                     /translation="FVVSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECL
                     CAKKRVVLCDAASYQNGLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            513..2276
                     /locus_tag="ACHBB7_000063"
     CDS             513..2276
                     /locus_tag="ACHBB7_000063"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM9208052.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            2360..2818
                     /locus_tag="ACHBB7_000064"
     CDS             2360..2818
                     /locus_tag="ACHBB7_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM9208053.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            2927..3610
                     /locus_tag="ACHBB7_000065"
     CDS             2927..3610
                     /locus_tag="ACHBB7_000065"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9208054.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            3757..4371
                     /locus_tag="ACHBB7_000066"
     CDS             3757..4371
                     /locus_tag="ACHBB7_000066"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM9208055.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            4387..4593
                     /locus_tag="ACHBB7_000067"
     CDS             4387..4593
                     /locus_tag="ACHBB7_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208056.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     regulatory      4818..4984
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            5132..5689
                     /locus_tag="ACHBB7_000068"
                     /pseudo
     CDS             5132..5689
                     /locus_tag="ACHBB7_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            5747..7786
                     /locus_tag="ACHBB7_000069"
     CDS             5747..7786
                     /locus_tag="ACHBB7_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM9208057.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(7895..9046)
                     /locus_tag="ACHBB7_000070"
     CDS             complement(7895..9046)
                     /locus_tag="ACHBB7_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM9208058.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(9200..9730)
                     /locus_tag="ACHBB7_000071"
     CDS             complement(9200..9730)
                     /locus_tag="ACHBB7_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM9208059.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(9829..10179)
                     /locus_tag="ACHBB7_000072"
     CDS             complement(9829..10179)
                     /locus_tag="ACHBB7_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM9208060.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(10336..11865)
                     /gene="ahpF"
                     /locus_tag="ACHBB7_000073"
     CDS             complement(10336..11865)
                     /gene="ahpF"
                     /locus_tag="ACHBB7_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM9208061.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(11875..12438)
                     /gene="ahpC"
                     /locus_tag="ACHBB7_000074"
     CDS             complement(11875..12438)
                     /gene="ahpC"
                     /locus_tag="ACHBB7_000074"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM9208062.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(12582..13244)
                     /locus_tag="ACHBB7_000075"
     CDS             complement(12582..13244)
                     /locus_tag="ACHBB7_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9208063.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            13428..15074
                     /locus_tag="ACHBB7_000076"
     CDS             13428..15074
                     /locus_tag="ACHBB7_000076"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM9208064.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            15065..16147
                     /gene="uxuA"
                     /locus_tag="ACHBB7_000077"
     CDS             15065..16147
                     /gene="uxuA"
                     /locus_tag="ACHBB7_000077"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9208065.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(16255..17808)
                     /locus_tag="ACHBB7_000078"
     CDS             complement(16255..17808)
                     /locus_tag="ACHBB7_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9208066.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            17956..19191
                     /locus_tag="ACHBB7_000079"
     CDS             17956..19191
                     /locus_tag="ACHBB7_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM9208067.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKPEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFAVLKRKINS
                     YQNPESHKTKLYCGAESFY"
     gene            19164..19244
                     /locus_tag="ACHBB7_000080"
     CDS             19164..19244
                     /locus_tag="ACHBB7_000080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208068.1"
                     /translation="MLWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(19370..20449)
                     /locus_tag="ACHBB7_000081"
     CDS             complement(19370..20449)
                     /locus_tag="ACHBB7_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9208069.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(20552..20992)
                     /locus_tag="ACHBB7_000082"
     CDS             complement(20552..20992)
                     /locus_tag="ACHBB7_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9208070.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(21049..21459)
                     /locus_tag="ACHBB7_000083"
     CDS             complement(21049..21459)
                     /locus_tag="ACHBB7_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9208071.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(21639..22073)
                     /locus_tag="ACHBB7_000084"
     CDS             complement(21639..22073)
                     /locus_tag="ACHBB7_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9208072.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(22173..23021)
                     /locus_tag="ACHBB7_000085"
     CDS             complement(22173..23021)
                     /locus_tag="ACHBB7_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9208073.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            23410..25176
                     /locus_tag="ACHBB7_000086"
     CDS             23410..25176
                     /locus_tag="ACHBB7_000086"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM9208074.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            25278..26537
                     /locus_tag="ACHBB7_000087"
     CDS             25278..26537
                     /locus_tag="ACHBB7_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9208075.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            26684..27985
                     /locus_tag="ACHBB7_000088"
     CDS             26684..27985
                     /locus_tag="ACHBB7_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9208076.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            27988..28839
                     /locus_tag="ACHBB7_000089"
     CDS             27988..28839
                     /locus_tag="ACHBB7_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM9208077.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            28852..29667
                     /locus_tag="ACHBB7_000090"
     CDS             28852..29667
                     /locus_tag="ACHBB7_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM9208078.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            29818..30984
                     /locus_tag="ACHBB7_000091"
     CDS             29818..30984
                     /locus_tag="ACHBB7_000091"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9208079.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            31214..31861
                     /locus_tag="ACHBB7_000092"
     CDS             31214..31861
                     /locus_tag="ACHBB7_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM9208080.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            31945..32889
                     /locus_tag="ACHBB7_000093"
     CDS             31945..32889
                     /locus_tag="ACHBB7_000093"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM9208081.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            33024..34475
                     /locus_tag="ACHBB7_000094"
     CDS             33024..34475
                     /locus_tag="ACHBB7_000094"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM9208082.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            34496..35332
                     /locus_tag="ACHBB7_000095"
     CDS             34496..35332
                     /locus_tag="ACHBB7_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9208083.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            35332..35991
                     /locus_tag="ACHBB7_000096"
     CDS             35332..35991
                     /locus_tag="ACHBB7_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9208084.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            36117..36806
                     /locus_tag="ACHBB7_000097"
     CDS             36117..36806
                     /locus_tag="ACHBB7_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208085.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            36812..37768
                     /locus_tag="ACHBB7_000098"
     CDS             36812..37768
                     /locus_tag="ACHBB7_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM9208086.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            37812..38324
                     /locus_tag="ACHBB7_000099"
     CDS             37812..38324
                     /locus_tag="ACHBB7_000099"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9208087.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(38580..39128)
                     /locus_tag="ACHBB7_000100"
     CDS             complement(38580..39128)
                     /locus_tag="ACHBB7_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM9208088.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(39217..40398)
                     /locus_tag="ACHBB7_000101"
     CDS             complement(39217..40398)
                     /locus_tag="ACHBB7_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM9208089.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGLGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(40634..42142)
                     /locus_tag="ACHBB7_000102"
     CDS             complement(40634..42142)
                     /locus_tag="ACHBB7_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM9208090.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            42301..42492
                     /locus_tag="ACHBB7_000103"
     CDS             42301..42492
                     /locus_tag="ACHBB7_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208091.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            42695..43612
                     /locus_tag="ACHBB7_000104"
     CDS             42695..43612
                     /locus_tag="ACHBB7_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM9208092.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            43605..44678
                     /locus_tag="ACHBB7_000105"
     CDS             43605..44678
                     /locus_tag="ACHBB7_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208093.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            44826..45344
                     /locus_tag="ACHBB7_000106"
     CDS             44826..45344
                     /locus_tag="ACHBB7_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208094.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            45325..45717
                     /locus_tag="ACHBB7_000107"
     CDS             45325..45717
                     /locus_tag="ACHBB7_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM9208095.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            45793..46728
                     /locus_tag="ACHBB7_000108"
     CDS             45793..46728
                     /locus_tag="ACHBB7_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9208096.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            46861..47640
                     /locus_tag="ACHBB7_000109"
     CDS             46861..47640
                     /locus_tag="ACHBB7_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM9208097.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            47633..47950
                     /locus_tag="ACHBB7_000110"
     CDS             47633..47950
                     /locus_tag="ACHBB7_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM9208098.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            48194..49804
                     /locus_tag="ACHBB7_000111"
     CDS             48194..49804
                     /locus_tag="ACHBB7_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9208099.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            49961..50809
                     /locus_tag="ACHBB7_000112"
     CDS             49961..50809
                     /locus_tag="ACHBB7_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9208100.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            51040..51519
                     /locus_tag="ACHBB7_000113"
     CDS             51040..51519
                     /locus_tag="ACHBB7_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM9208101.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            51552..53780
                     /locus_tag="ACHBB7_000114"
     CDS             51552..53780
                     /locus_tag="ACHBB7_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9208102.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            53909..55552
                     /locus_tag="ACHBB7_000115"
     CDS             53909..55552
                     /locus_tag="ACHBB7_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9208103.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(55625..56170)
                     /locus_tag="ACHBB7_000116"
     CDS             complement(55625..56170)
                     /locus_tag="ACHBB7_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM9208104.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(56193..56678)
                     /locus_tag="ACHBB7_000117"
     CDS             complement(56193..56678)
                     /locus_tag="ACHBB7_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9208105.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(56668..57480)
                     /locus_tag="ACHBB7_000118"
     CDS             complement(56668..57480)
                     /locus_tag="ACHBB7_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM9208106.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(57619..>58727)
                     /locus_tag="ACHBB7_000119"
     CDS             complement(57619..>58727)
                     /locus_tag="ACHBB7_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM9208107.1"
                     /translation="ILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAHS
                     MGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKVV
                     HMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTSM
                     AQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLPE
                     NTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLIE
                     TINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELPR
                     LNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        19654 a        10577 c        11396 g        17100 t
ORIGIN      
        1 ttttgttgtt tcagaaagtt cagtcaaaca atttttttat cgaacccaat ttcatttgta
       61 tgcggaagaa ggcatcgctg aaataaatcc tattttattt cgttcattag aagaattgcc
      121 tgatgaatgt ctatgtgcga aaaaaagagt ggttttgtgc gatgctgcta gctatcaaaa
      181 tggattagaa actgaaaaga caaagatcta tccaatctca ttaaaaggtg caaacatcca
      241 tttgtatcat attgctcagg aacttcacct attaaaaaaa catatttctt gatgagacct
      301 taattaggag accttctaca aagcttttta attgtctgta gaaggttttt atttgagtcc
      361 tgattcgtgc gaagatctac ttatataaca agctttctct gaaatttttt taaaatgcta
      421 ttgacttaga gtcaactcta agtagtagaa tagataaagt gtaacaaatc tattttcatt
      481 aaaagaagat agtacggagg agagaatgta ttatgagtac agtattacat gtattggatg
      541 caatcaatag ccccgcagat gtcaaaaaat tatctgttga ggaaatgaaa cagttggcag
      601 aagagatgcg ccaaaaaatc ttgcaaaagg acagcgatgt aggtgggcac gttggtccga
      661 atctaggtgt agtagaatta acgattgcgt ttcattatgt atttgattca ccaaaggata
      721 aaatcgtttg ggatgtttcc catcagtctt atggtcataa gcttttaact ggtcgaaaaa
      781 atggttttga aaaaggacat taccgtgaag tgagcggata ttccaaccaa catgaaagta
      841 aacatgacta ttttacagtg gggcacacat ctacatctat cagcttagca acaggtttag
      901 ctaaagcgag agatctgaag aaagaatcag gaaatgtggt agcatttatc ggtgacggct
      961 ccctaagtgg tggcttagct tttgaaggat tgaacaatgg tggtgtttta caatccaatc
     1021 taatcgtatt agtaaacgat aatgagatgt ctattgatga gaatcatggc agtttatata
     1081 aaaacttagc tgagcttcgt caaaccaacg gtaaaagcag caataaccta tttaaagcaa
     1141 tgggatttga ttatcgttac gtggataatg gaaacgatct ggaaacggtg atccgtttat
     1201 ttgaagaagt aaaagatatt gatcatccga ttgtacttca cgtccatacg gaaaaaggtc
     1261 gcggttatca gccggcaatc gataataaaa tgaaatatca ttggcatgta ccatttgatc
     1321 tgtccaccgg ggaggcaaag caagtaactt ctggagaaac atttaatatg atcatcatgg
     1381 attatttgga taagaaaatg caaacaagta cagctcctat tgccgcagtc aatgcagcga
     1441 taccaggcgc ctttggactg aaaacattcc aagaaaaaca tccagatcat tattttgacg
     1501 tggggatcgc tgaacagcac tctatcactt tcgcttctgc tttggcttct cagggagtac
     1561 gtccagtcgt cttccaatca agtactttct tgcaacgggc atatgatcaa ttatcacatg
     1621 atttagcgat caacgagaat ccagtgatta ttttagtcgg tggcggaacg atctcagcta
     1681 gtgaccgaac acaccaagga acatttgaca ttccttactt atcaagtatc ccgaacatca
     1741 aatatcttgc tccagcaaca aaagaggata tgatctctat gctagattgg gcgttcgaac
     1801 aaacagcgca gcctgtagca atccgaattc ctacacatgg tgtagaacat ggtacagcta
     1861 tcgcaaatga ttatagtatt ccagcctatc aaacagtaaa taatggagga aaagtagcaa
     1921 ttttagcttt aggcggtttt cttgaattag gaaaagaaac agtaaaagct tttagaaaaa
     1981 aaggaatcga agcaacgttg atcaatccag gaacgatcac agaaaatgac gtagaaacat
     2041 tagataaatt aaaagaaaca cacgaattag tcatcacatt agaagatggt agtttgtcag
     2101 gaggatttgg cgaaaatatc agtcgttatt atggtgattc ttcaatgaac gtattaaatt
     2161 ttggtgcaga gaaagaattc acggatagcg ttcctgtagg gcagctttat gaacgttatc
     2221 atttaacacc aaaacaaatc gttgaagata gtctgcgtgt cttgaatatt caataattca
     2281 ttatgctcac catccagata attgtttata ctagtatctg gatggttttt tcattataaa
     2341 aagcaaaggg gtggatgtta tgtatcagca agtagaagaa tatgtcaaaa agttccaaga
     2401 aaaatatcct agttatcaat cagaaaaatt aggtttcttt tcatttgggg atacagaaaa
     2461 aatggcagac gagttgagcg aactagttgt aaaaaagata aagactggta catcaagtgg
     2521 ttacgatctg tataaacaag agaatgagcc tttaccaaaa gttggtcaat tagacgtggt
     2581 cctgaatgga aaaaatgaac cagtttgtat catcaaaaat ataaatgtga ccatcttgcc
     2641 ttttagtgaa gtcacgaaag agcatgcttg gaaagaagga gaaggggatc ggacattagc
     2701 ttattggcgg aaagtccatg ctgagttttt taaacatgct tataaagaag cgttgaacat
     2761 agaatttaca gaagagaagc tggtcgtata cgaagaattt gaagtaattt tcttttagta
     2821 agaaaggtct gctgaaagaa atagctcatt attgtctcct gaatagaaaa gggttacatt
     2881 tttatatgaa gctgtatttt tatataacat tactggagga gatattgtgg atacactgga
     2941 acaaatcaaa ggcggagtcg tcgtttcttg tcaagctttg gaaaacgagc cgctacacag
     3001 ttcttttatt atggggagaa tggctttggc tgcaaaagaa ggcggagcag ttggtattcg
     3061 agcaaatacg acagaagata tcaatgaaat caagcgaatc gttgatttac cggtaatagg
     3121 gattatcaaa agagattacg atgggtcaga agtgtttatt actgcgacag aaaaagaagt
     3181 ggaagaactt ttggagacgc aagcagaaat cattgcactg gatgcaactt ctcgtaaacg
     3241 tccgacagaa aaaacaactg ctgaacttgt ccaaatgatt cacgaacacg gtcggttagc
     3301 tatggcagat atttcgactt ttgaggaagc gcttcaagcg caagaagatg gttttgatct
     3361 tttgtctacg actttagcag gctatacaga atattcacga aagacgcaga cacctgattt
     3421 tgatttattg aatcaaatca tagaacaagt cgatatccca gtaattatgg aaggacatac
     3481 tgactctcct gaacaagtag agaaagcata tgaattaggt gctttttcag tcgtagttgg
     3541 atctattatc acccgtccgc aattgatcac taaaagatat gtcgaagccg caagtaaagc
     3601 aaataactaa aaaacacgca cggcacagat tcttgacaaa aaagaaaaat tgatagatag
     3661 tttaaagcag attctaagaa gatgaaaaaa gcattacttt tcattttgtc ttcaagatct
     3721 gctttttaac gaattttata gaaagaaggc gaaaccatgt ttgaacggag tccttcagaa
     3781 ttgacagaac gtgaaaatta caagttgctg attggttcag tcatccctag accagtagca
     3841 gtagtaacga ctaaatcagc aaaagatata gtgaatatcg cacccttcag ttacttcaat
     3901 atcgtcagtt cgaatcgacc catcttatcc ttggcgatcc agagaaaaca agatgcagta
     3961 aaagacacag caaagaatat tcttgagaca agagaagctg ttatccatat ccttgatgag
     4021 gacaacgtag aagaagtaaa taaaacagca gccaatctgc ctaatgagga aagcgaactg
     4081 tacctgactg acttgacttt aactgattca gtaatcattt cagtaccagc attgacagaa
     4141 gcaaaagttc gttttgaaac atccttatat gaacatgtcg agatacggaa taaaaataaa
     4201 gtaacagctg atttattttt gctagaagta cagaaatatc atttaaatga aacagtctat
     4261 gatgataaga caggaaggat cgatccaaga aaattgaatg ctgtaagccg tttggcagga
     4321 aatgattatg cagcaatagg agaaattttc acgattgcac gtcccgaata aaaaggagca
     4381 ctagttatgt taaaaataag ctataaacca tcgacagatt caaaagaaat gaaaaaagaa
     4441 tatgaaacag tgaatgattt tctgcaaggg cagtacttag aagttccgcc attgcaagac
     4501 cattttgtcg ttactactgt tactttagat ggtaaggaaa tagaaatgcc agatcaaacg
     4561 attagcggat tgtttaatta ctttaacaaa taaatattta ttccgaaaag tgaaaaaaca
     4621 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
     4681 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
     4741 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
     4801 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
     4861 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
     4921 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
     4981 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
     5041 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
     5101 gagatgcggt gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca
     5161 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
     5221 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
     5281 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
     5341 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
     5401 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
     5461 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
     5521 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
     5581 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
     5641 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
     5701 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
     5761 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
     5821 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
     5881 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
     5941 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
     6001 taaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
     6061 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
     6121 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
     6181 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
     6241 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
     6301 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
     6361 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
     6421 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
     6481 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
     6541 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
     6601 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
     6661 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
     6721 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
     6781 gacaatcgat gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc
     6841 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
     6901 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
     6961 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
     7021 aatgattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
     7081 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt
     7141 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
     7201 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
     7261 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
     7321 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
     7381 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
     7441 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
     7501 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
     7561 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
     7621 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
     7681 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
     7741 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
     7801 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
     7861 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
     7921 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
     7981 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
     8041 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
     8101 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
     8161 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
     8221 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
     8281 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
     8341 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
     8401 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
     8461 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
     8521 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
     8581 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
     8641 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
     8701 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
     8761 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
     8821 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
     8881 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
     8941 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
     9001 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
     9061 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
     9121 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
     9181 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
     9241 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
     9301 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
     9361 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
     9421 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
     9481 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
     9541 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
     9601 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
     9661 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
     9721 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
     9781 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
     9841 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
     9901 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
     9961 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
    10021 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
    10081 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
    10141 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
    10201 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
    10261 aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt
    10321 tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc
    10381 tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc
    10441 gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt
    10501 catttcgatc gttccatcta gccaatccgt attagggagt aagccgacta gaatgaatac
    10561 tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc
    10621 tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc
    10681 aagagagact agtttatcct gtaagatttg atctgctttt aattctggta agaattctag
    10741 aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc
    10801 accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata
    10861 agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc
    10921 acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt
    10981 aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg
    11041 gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt
    11101 accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac
    11161 aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc
    11221 accgccaatt actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg
    11281 gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc
    11341 atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat
    11401 cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc
    11461 ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt
    11521 ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg cccgtccact
    11581 aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa
    11641 aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc
    11701 tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag
    11761 tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag
    11821 tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat
    11881 ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg
    11941 acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc
    12001 actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg
    12061 atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat
    12121 acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc
    12181 tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc
    12241 aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca
    12301 aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt
    12361 ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt
    12421 tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat aattattgta
    12481 atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa
    12541 cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg
    12601 ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt
    12661 tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt
    12721 tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct
    12781 aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa
    12841 caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact
    12901 gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg
    12961 gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct
    13021 gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt
    13081 tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt
    13141 tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt
    13201 ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa
    13261 tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt
    13321 aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgtataa
    13381 aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa
    13441 acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg
    13501 accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg
    13561 gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat
    13621 tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc
    13681 aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac
    13741 gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg
    13801 ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg
    13861 aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata
    13921 taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgcg ctactttata
    13981 aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa
    14041 acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt
    14101 atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga
    14161 cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag
    14221 agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg
    14281 tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga
    14341 aattcgaaaa agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga
    14401 tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac
    14461 tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg
    14521 aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga
    14581 tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct gatatgcctc
    14641 agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa
    14701 aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg
    14761 ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc
    14821 cagacccact attgacagac tttcagcata agctttccga tattcaactg ggtacgcaag
    14881 atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg
    14941 tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag
    15001 caggaccgaa tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt
    15061 tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga
    15121 aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg
    15181 agaggtatgg cccagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt
    15241 aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc
    15301 ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat
    15361 agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta
    15421 ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc
    15481 aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttgaattgc cgggatggga
    15541 acctgagcgc ttagctcata tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga
    15601 aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt
    15661 aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg gattacctcg
    15721 aatcgtaaag aatcgagaag atttagattg gttgtgccgc atagtagaca gcccttataa
    15781 cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat
    15841 tttagcagaa ttttgtcaaa gagatcgaat cccatttgca catgttcgaa atattaaatt
    15901 tttagctaat cgagatttct atgaggcacc gcataaatcc gaatacggtt cattagacat
    15961 gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac gaatacatcc gtcctgatca
    16021 tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt
    16081 aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga
    16141 taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga
    16201 tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga
    16261 agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat
    16321 atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa
    16381 aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat
    16441 gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc
    16501 tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac
    16561 aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg
    16621 cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat
    16681 ataaaattgt tcaatggcgg tttggacttc tttcaacacg ctatgttctt gataaagcat
    16741 gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt
    16801 tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac
    16861 agtacaaaag gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt
    16921 taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg
    16981 agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat
    17041 atctacagag ggatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac
    17101 gtctttcaca gaagttgcag gaaaattctc tttacatatt tttaatagaa aatataattt
    17161 atctatcata ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat
    17221 aaaagccact acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt
    17281 ccaataaata tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct
    17341 gccattatta gaaaaggaga tagacaaacc gtacaatgct aaaaatgcat taatcttttt
    17401 cattttcgta tacaaataag aggcactata gtttgtttgc tgacttaaac tgataatcga
    17461 atttgtctga cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta
    17521 tcttccaaat agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa
    17581 aaaaggtttt tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc
    17641 taattctttg atgtatagta aaaaatttga ttcagacata tggaaatgat ctgctaaata
    17701 ttgtgtagta ataaagaaac gatgttcaga taggaaaaga aaaatatcta attttgtatt
    17761 gctttttttc ttcaagagaa acgttttaat cgcttcttga gtttgcatga aagaggctcc
    17821 tttttactta aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa
    17881 aaaacagtaa acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca
    17941 aggagtgaga gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc
    18001 ttatcgctta ctataaatca gacagtccac acagaggaga tagaaacagc caaatggaca
    18061 gaaaccaagt gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat
    18121 tcaggagtcg caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat
    18181 tcaggagact tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccgct
    18241 ttgtcgattg gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc
    18301 ttcaccgtta cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca
    18361 gatggactac cgaacgatca tcatcattta acgccgtata gtcagcggca gacaattgag
    18421 caagattcag ctatcgactc aggaggagat cttgttgaag aaccaacaga taaaccagaa
    18481 aatgaaaata agccagaagt accgcctaca gaaaatccag acggagaaca gaaaccggaa
    18541 atagaacctg gagaagaacc cgatacagaa actcaaccag agccagacaa tgaatcaaaa
    18601 cctgaaataa cccctggaga aaaaccagat gtagacccag aagaaaaacc agatgtgact
    18661 ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc agacacagat
    18721 aatgaaactg aaaatccaga aaaaccagag gtagccccag aagaaaaacc agatgtaact
    18781 ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc agacacagat
    18841 aatgaaactg aaaatccaga aaaaccagag gtagatccag aagaaaaacc agatgtgact
    18901 ccagaaccag atacggacgc aagagaccaa ggaataccag aaaaaataaa caaaaaaaca
    18961 attcaagagg atggaaaaaa agagagtaaa aaatcaaatc tagcaattct aaaaataaat
    19021 gaagagcagc tgaataaaaa atcacgaata tttgatagcg cccaatcagc tgaaacctta
    19081 aaaagtagca aggatacaac ctttgctgtc ctgaaacgaa aaataaacag ctaccaaaat
    19141 ccggagagtc acaaaacaaa gttatactgt ggagcggaat cattctatta agtatagcaa
    19201 caatgctttc tgcaaaaaga tttaaacaaa acagatctct ctaacatttg aataatgtct
    19261 gtgaacgcat ttttcaaata aaaagaaata tccagaaaat gaccccaaaa taccagattg
    19321 aaatctgata tttggggtcg ttcatacttt atctttcttc atatttattt taagggatat
    19381 ctgtttcttt tggaagatgg ttcgggatct ttttcacttt caatacatct aaaaagaagg
    19441 taaagatcgg aatgccgaca atcaagcccc aaacgccaaa gaaccgttcg ctaacaagta
    19501 agaccacaaa tgtataaaaa atcggcagtt ctgttttgct ggacatcaac ttcggattca
    19561 gcacataaga ctcaattaga tggacaatca aaatcgtaat caggatataa acaacatctc
    19621 ggatgcctcc ttgggaataa gctatgaaag atagaggaat acaagaaatg ataacacctg
    19681 ctactgggat cagacttaaa atgaaaatca tgatcgctaa actcagcagc tgatgaaagc
    19741 caaatacagc caaggcgatt gtagtgagca acgtatttaa aagagcgaca acaaattgag
    19801 cttctaatac caaaccaaat gtattgacaa acttatcggc aaaatagtaa atatcttgaa
    19861 agaaccaaga aaattcactt tttaaaaaca atcttgagaa tacaattgtt tttttctttt
    19921 caatcataaa aaagaaactt aagatgaatg acatagcaaa cgccactgcc atttttccta
    19981 tatcctgtaa atagccaagc gcaatcgaag cgccattttg aagctgttca aaaaaatcgt
    20041 ttcgctcaat ataagtatgg atatattgta aaacaggatc tgcatctttt ggctgatgct
    20101 cgtaaaaatt aataacggac tggatcatgt caaatgtttg atgaataagc atcgggatat
    20161 actttgtgaa tgccagatag ataaggtaaa cgatcagtgt ataaatcatg atagctaaaa
    20221 cacctgtagg gattcggaca aatcgctgaa tgatttgaac aagatgaatc atcaaataag
    20281 taaagataaa tgtgagcaaa atggttgtaa tcatacttct aactaagaaa agaacaaaaa
    20341 cgacaaataa taaaacagtg agacggcgta ttttttcatt cgccaaaaaa cgctgataaa
    20401 cggtcaaatc gacaacctcc aaataacaaa aaataaacac gttattcatt atatgatgga
    20461 atatgaaaat aagaaagagc aaaaagaaaa accgctaaat gacgttatct tatgagttgg
    20521 ttttcctcat aaaatcgttc atctagcagt ttcagccctt tttctggtct tgatgaaaat
    20581 tatctagcat ttttcgggtc agatgttgca gtgtatccaa ttcttcctgt tcaagtggaa
    20641 gataagactt gatcttttgt ggaatacctt ctgcttgttt ttgcaacgca gcaccttgag
    20701 cagtcagcgt aacatttacg attctttcat cacccgtttc acgctgacga ttaatataat
    20761 ttttttctgc taactttttt agtaagggag tcagcgtacc agaatccaga cacaaatcat
    20821 ttcctagctc tttcactgaa gtctttttct tttcccataa aactaacatc acaagatact
    20881 gggtataggt aagattcagc ggcttgagga ggggagtgta gtgtctaatg atttctttag
    20941 aggcggcata taaaggaaaa catacttgat taactaaata tagatcttcc atttttccac
    21001 ctccgctgtt ttactatagt gtatcatagc ggatttacac aaaaaagtct attcactaac
    21061 tgctttcaga gtaacttcta tatttccagc tgtagctttt gaatacggac acactttatg
    21121 cgctgcctct aataattctt gcgtttcttc cagagaaaga ccttcgatag atccttcaat
    21181 ctctacacct aagacaaagc tcgtttcact ttctgaataa agggaaactt gtgcactgac
    21241 agtagaagcc ccagagatat ttttttgctg tttgatcaat tctaatgcac tgttaaaaca
    21301 tgcactgaaa ccagctgcaa acaattgttc agggttggtt gcattttcga cgcgtttacc
    21361 aggagctgca atattcaatt cgaatgtacg atcgggatta tgcacttctc cagagcgtcc
    21421 gcctgtgttg atcattttcg tagaatacat ttttttcata aaattaccac ctttaattat
    21481 attttataca attgaatttt acacaatata attgtggaaa gcaaagaaat ggattggcat
    21541 aagataaata agagaaagca catgaaacag acgcgctttc tcgaatagca aacatataca
    21601 aaaaatatca gagacaaaaa ataatctcat cataaacatt acatatattt ctcaaaaagt
    21661 tgttttcttt cttcaaaaat tacttcacga tggacctgat gaagcatcca taaataacca
    21721 aaaggatctc tgaacattga gttagtcaca ccaaagtcca tcatttctgt catttcttga
    21781 atctctacac agccatttcg taatgcttta ttatgtgtgt cctcgatatc agagacgatg
    21841 atattgaacc agatcgtatt tggatgttca gcagtaggag cagtcaaacc aagagacgtg
    21901 ttttcatcca gcatatggaa tctcgtacca tacaaggtaa aaatgacttc attattgcct
    21961 ctagggtaat ctgttgcctc aatcagttgt atttcatcaa agattgtttg atacagttct
    22021 agtgctttca aactatcatt taatacaaaa tcgatttccg ttcctttatg cataaagatc
    22081 tcctctcctt ttcagtcgaa cagttactag attcttttat tataccagct aaagaaaacg
    22141 cattcaaaaa aatagcggat ttacttacca actcattcga ttggatgata agtaaatccg
    22201 cgtcccatta cttctgctac gctgctgaca atgacaaagg cacttggatc gatttcttct
    22261 atcaatggtt tcattcggga aaattcatga tcagtcacaa cgatcaataa aacctctttt
    22321 gactttcgat tatacccgcc ttttgcctct agcagggtaa ggccacgtcc aatctcagca
    22381 cttaagcgaa ggcggatgtc ttccaaataa tgttcactca tgaccataac agatttcttt
    22441 cgattgatac ctgtttcgat ataagtcatg acaattgaag tgattgcaat agataaaata
    22501 gcataaagaa actcttctat tccaaaaata aacagattaa gagaaacaac gaatgcgtct
    22561 gtaaaaaata agccaatcga agtattcaag tggaaatatt ttttaaaaat gaggggaggg
    22621 atcgtcgtac cgccgctaga agagttgttt ttatataaaa tcgcaactcc taaagcaaaa
    22681 atcgcacttc caaaaatgac agacaacaac cgatctgaag taatcattgt ttctggaact
    22741 attgcaatga ttaaaggaaa aaccaagctt ccgaaaagta ctttaaaaaa gggtttttta
    22801 cctaaaaata gaagagctag taccaacata acgatattca acactaaaat aactatagct
    22861 cggtcaaagc ctaaagcagc ttccagtaat attcctagac cgctgacacc tccagcagca
    22921 atatgatgag gccctaaaaa catattgata gcgactccta atatgaagag ggcaaaaata
    22981 atcttaatgt atgtccatat aaattgtctc gtttctttca tttattcact tccttaattc
    23041 attaataata ctcttatctt attataactt acttttcacc ttaagttaac tttaattaat
    23101 aaaaaaatta ggctagtcta aattattatg tttcattctt ctagaatata cttctacgcc
    23161 aaaaactgtt aaaaaacttt tactttagaa aattggagaa attgatcgct taagataaat
    23221 tcaggacact aaatagtaca ggaagtagac taaaactttt aaaagtttta agtctatcta
    23281 tttggcaagt ttaaaaaata aaaattcggt atcaaaaaac tgttaccgat aacatttttg
    23341 aaagcgttgt cttttgcttc tctatcactt atagtgatga cgtcatatta aaacacaagg
    23401 aggaaatcaa tggatacagc tgcaatttat catcgaccgg aaagtgaata tgcatttcta
    23461 tatacgaata caagatttcg tattcggctt cgtactagga aaaatgatat caaaaaagta
    23521 tatgtgctat gtggagatcc atataccata acaactgaaa aatggtatca aaaacaacga
    23581 ccaatgaaaa aatgtctaag taccaatgtt catgattatt gggaaattga agtgacgagc
    23641 gaaacaagtc gcttacagta tgcgttccat gttgttgggg aagataacac ggactgtttt
    23701 tacggtgatc aaggaatttt tccttatcga gaaaatgtga ttactgaacc aaatttttat
    23761 tttcgaatac cttattttca tcaaatcgat cgattcacta ttccagaatg ggtaaaagaa
    23821 acagtatggt atcaaatttt tcccgaaaga tttgcaaacg gggacaaagc aaatgacccc
    23881 gaacacactt taccttgggg aagtaaagat ccagaccgag aagacttttt cgggggagat
    23941 ttgcaaggtg tgatggatca tttggattac ttgacggacc ttggcatcaa tggcatctat
    24001 ttttgtccca tatttaaagc cacatccaat cataaatacg atacgattga ttattatgag
    24061 gtggatcccg cttttggcga taaaaaactt ttaaaaaact tgattgatga agcacataaa
    24121 agagggatac gtatcatgtt cgatgctgta ttcaatcata tgggtgtcca ttcaccacag
    24181 tggcaagacg ttctcaaaaa cggagaaaaa tcgatctata aagactggtt ccacatccgt
    24241 tttttcccag tagattctta tcagatgact gacttaccag aaactgctga aaatatccca
    24301 tatgatactt ttgcttttac tccattcatg ccgaaactaa atacagcaaa tccagaagtg
    24361 caaaaatatt tgctggatat cgcaacttat tggataaaag aattcgatat tgatggatgg
    24421 cgtcttgatg ttgctaatga agtcgatcat catttttgga aaaaattcag agaagcagtt
    24481 acagaaatca aaccagacat ctatatcctt ggagagattt ggcattcctc tcaagcttgg
    24541 ctgcaagggg atgaattcca tgcagtgatg aattatgcat ttacagattc gattaaagat
    24601 tattttgcaa aaaagaaaat cacagcttca caaatggtca gtggaatgaa tcatcagcaa
    24661 atgctttaca gagatcaggt gaacgaagga acattcaact tattagattc tcatgatact
    24721 gccaggatct tgaccctttg ccaaggtaac aaagaattga tgaagtctgt gatggccttt
    24781 atgtttcttc aaaaagggtc tccttgtatc tattacggga cagaaatagg gatgactggt
    24841 gaagatgacc cggattgccg caaatgtatg atatgggaga aggaggaaca agatctagag
    24901 ctattcggtt ttataaaaga acttgtttct ctaaggaaac agttgtctaa gattatttct
    24961 gaaggttcta ctcagtggct aatagtaaat gatcgtgaag ataagctcta tttcacacgg
    25021 aaactagagg gacagatcat ctatgtttac tttaatcagt caaaagaacc ttgggtagta
    25081 gaacaggaaa atgaggtgat tcttagtcaa aattgtcagt tgttagaaga cgggaaagca
    25141 gagatacgac aatacggatt tttgatcatg tgttgaaaac tgttaccggt aacaacaaga
    25201 aagcgtttgc aaatgaagat ttgtgcccat tataataagg gtgtaacagc acgttgaata
    25261 caaaggagga aatgaagatg aaaaaaagaa gtgtgaaaca gttgggttta gggttaatct
    25321 ctttaggaat cttaggcgga ttagcggctt gtggcaatgg taactcttca gcagaagggg
    25381 aagccgatga aggaaaaacg ttaacagttt ccgttgattc tggatataaa gattatataa
    25441 acgaaataaa aaacactttc gaaaaagaaa acgatgtgaa aatcaagcta gtagaaaagg
    25501 acatgttcga tcagttggaa tcattagcgt tagatgggcc tgctggaaag ggacctgacg
    25561 ttatgatggc agcatatgac cggatcggtg cgttaggaca gcaaggacat ctggcggaag
    25621 taaaactggg aaatgaagca gcttacgatg aaacagacaa agcccaagtg acttatgatg
    25681 gaaaaatcta cggagaacct gcagtaatcg agacccttgt tttgtactat aacaaagatt
    25741 tagtcgatac agcaccatca acgtttaaag atttggagaa tctatccaaa gacagtcgtt
    25801 ttgcttttga gtcagaagca ggaaaaaata caggattttt agctaaatgg actgactttt
    25861 attattccta tggtttaatt gcaggttacg gtggctatgt atttggtgat gatggcacaa
    25921 atccttcaga tatcggtttg aataatgcag gtgctgtaga aggaatctct tatgcaacag
    25981 attggttcaa aaatgtttgg ccaaagggaa tgcaagacat aaaaagtgca ggagacttcg
    26041 ccagccagca attcatgtcc aataaaactg cagcaatcat tgacggtcca tggcaagcgc
    26101 aaacatacaa agaaaacaat atcaattatg gtgtagccaa aattcctact ctaaataatg
    26161 gacagcctta tcaaccgttt ggcggaggaa aaggatgggt agtcagcaat tatgctaaaa
    26221 acaaagacct ctctcaaaaa tggcttgatt atgtaacgaa tcaggaaaat caagaaaaat
    26281 tctttgaaat ggtaaatgaa atccctgcaa accaacaagc tagggaaaca gcaaaggcta
    26341 aaaacgatga attaacaaca gctgtgattg aacaatatga aacagctcaa gcgatgccaa
    26401 acatcccaga aatgggcgaa gtttggacag gtgcagaaaa cttgatgttc gatgcagcat
    26461 caggttcaaa aacaccaaaa gaatccgcag atgaagcagt aaaaacaatc agtgaagcca
    26521 tagaacaaaa atactgatta aaatgataac cgtccatttt tataaaaaat ggaacaggtg
    26581 cttcagataa aagtggcaag agcgagatgg aaaattatct tgctcttgtt gccattatct
    26641 taaaagcagt ttgcggttta gcaagtttag agggggcatt cagatgcagc aagataaaac
    26701 aagcaatatc aacgaaaaaa aaatcacgct ttgttcactt attccaggga tggggcagtt
    26761 tgtaaatggc caagctttta aaggtatcgt ttttttcgcc atattcgtcc tattcattgg
    26821 tcagatgctt ctgggcggat ttcatgcatt ggtaggattg gttactttgg gaagtatacc
    26881 aatggaagat cattcattat ttattttgat tgaaggcact ttgcaattca tcattacaat
    26941 cattttcttg attttttacg gtttgaatct tttagacgct tatcgagtag cgaagatgcg
    27001 tcaagagggc aaaaaaatca atcggacgat gaaggaagtc atacataata taggtgatga
    27061 aggttttcct tatctattga cacttcccgc ttatcttctc atgattttta cgatcatttt
    27121 cccagtccta gttacattgt tcactgcatt tactaattat gacttcaatc atattccacc
    27181 agccagttta attgattggg taggggctaa aaactttttt agtatcttct ttttaagctc
    27241 atatcgtaat actttttttg cagtcttttc gtggaccgtg atttggactt tatgtgcaac
    27301 aactttgcaa atcacccttg gtattctcac agcggttgtt gctcaccaga aatttattaa
    27361 attcaaacgt attttcggta tcattttctt attaccatgg gcggtcccag cttttattac
    27421 tatcatgagt ttttcgaata tattcaacga tagcgtaggt gccatcaata ctcaagtgat
    27481 tcctttcata aaccagcttc cttttgtaga tatcagtgca atctcttgga aaacggaccc
    27541 gaactggacg aaagtagcaa ttatcttagt gcagggctgg ttaggatttc cctatatcta
    27601 tgtgatggtt acaggaatct tacaatcgat ttcagaagac ttatatgaag cagcaaaaat
    27661 cgacggagca aatgcatggc agcgcttttc aaacatcacg cttcctgctg tcttcctagt
    27721 ggcagcgcca acatttatta cacagtatac ggggaatttc aataatttct ccatgatcta
    27781 tctgtttaac aatggcggtc ccggaagcgt aggcggagga gctggttcta ctgatattct
    27841 tatttcctgg atctataaac ttacgactgg aacttctccg cagtattcta tggcttcagc
    27901 aatcacgttg attatctccg tcatcgttat ctgtgtgtca ttgctggtat ttaaaaagac
    27961 aaatgcgttc aatatggggg attaacaatg aaaaaatttc attcgagtgc tcgattccaa
    28021 aaacgacttt ctcacttttt tacttacttt tttcttttaa tactgtcagt cattatcatc
    28081 tatccgcttt tgatcacagc aagtactgcc ttcaagtcag gaaatattgc agcttttagt
    28141 ttagatcttt caagtgactg gactttagga aactttactc ggctattcag tgaaacactt
    28201 tacgggactt ggtataaaaa tacattgatc atttctgtcc taacaatgat cgtacaagtg
    28261 actgtagtaa ctttggcggg ctatacttat agtcgctatc gttttatcgg ccgcaaatca
    28321 agtttgatgt tctttttagt gattcagatg gtaccgacta tggcagcatt gactgccttt
    28381 tatgtcatgg ctttcttatt agaagcgctc gataagtact ggttcttgac aatgatctat
    28441 ataggcggtg gaattccaat gaatacttgg ctgatgaaag gatactttga cactgtacca
    28501 atagatttag atgaatctgc taaacttgat ggggcagggc atttcaggat ttttttccag
    28561 attgtattac cgcttgtcaa accgatgctc gccgttcaag cattatgggc ttttatggga
    28621 ccttttgggg atttcatgtt agctaagttc ctgcttcgaa cacctgaaaa tcagactgtt
    28681 gctgtcggct tacaaacatt tatttctgat gtgacaaacc aaagagtctc tctttttgct
    28741 gctggggcaa ttttgattgc actaccgatt tgtctattat tcttcttttt acaaaagaac
    28801 tttgttaccg gcctgacatc tggcggaaca aaaggatagg agcagagtat catgcaaaca
    28861 tttccgttaa actatttttc cgcattacgt acgccaactc aagtgtttgc gggacgcaaa
    28921 ctattatcat ggcctaaatt tttcttgata tttgtatttt tagtttcctt gatggtcatg
    28981 cctgtgactc ttttttatgc caatcagatc caagcgattc cattagaaca atttctatcc
    29041 gttcattcgc tgatcgatga acaaggaacc caaaaatttt ctgaacttga attatctgaa
    29101 actgggttac aagcatcgca acaaacgata gctgtgaccc ctgaaatttt ggtaggtgtg
    29161 tctttatctg agaaacaaca atccgatcat ggaactttta ttgattttga gaaggaacaa
    29221 tgggtgatcc aacaaaagga taaatctggt atacgccgct atacgatgaa ttatagccca
    29281 tctttccaac cagattctgt acgtacgccc gaagatttcc aacgattttt agaaagagaa
    29341 ttttatgcca gtaatcgtcc aacaatcatt ttatcttata gtttaagtct tggtcttgtc
    29401 ctatttgtca tgacgagtct cattttattc ggggcttcct ttttcttgtg gatgacaaga
    29461 aaaagccaac tttcatccat tcatacattc aaagagagtg caaatttgat gctgaatgtt
    29521 atgggaatag gttccatggc agcagcagtt gtagggttta tccactttga ctttatcttg
    29581 atgctaagtg tccaaacaac gataactgtc ttgctgcttt tatgggtttt tgtcaaaaca
    29641 agattcaaag ataagcgtgt cgaatgacaa gtttaagcga tttatgcgac aatgaatgtg
    29701 aaaaaaatga aaaataaagt agtttttgaa aaatttctga aaaaattaaa cttcttagtt
    29761 gattcttttc ttttttagta ttaacgtaat agggtagttt ttttaaggag gaaaatcatg
    29821 ttagagaaaa ctttatacaa tcaattattt tcacgttctt tctcgctccc tgtcgaagta
    29881 aattattggg acggtacaac gaaacaatac ggagatacgg acactcctcc ccaaatcaaa
    29941 atcaatatcc atgaagaaat cccaatgaaa gagattacaa gtaacgcttc tttggcacta
    30001 ggagaagcct atatggatca tcgaatcgag atcgagggaa gtatccaagc attgatcaac
    30061 gatgcttatc agcaagcaaa cagcttttta cgaggaaaag attatctaaa atggctgcca
    30121 aagaaagaaa aacatacgaa acaaaaaaac aaagaagata tccatgcgca ttatgactta
    30181 ggtaacgact tttataaact ttggctagat ccaactttga cttattcttg tgcttatttc
    30241 aatacacctg aagatacgtt ggaacaagca caaatcaata aagtccatca tatcttagac
    30301 aaactcttta tcaaagaagg cgaaacgttg cttgatatcg gttgtggctg gggaacattg
    30361 atgttcactg ctaccaaaga gtataatgtc aaggcaactg ggatcacact aagtgaagaa
    30421 caatacgatt tgattaccaa aagaataaaa gaagaacatt tagaagataa atgccgtgta
    30481 ttattaatgg attaccgcga actaaaaggc gaaacgtttg atcatatcac aagtgtaggt
    30541 atgttcgaac atgtaggttc tgaaaatctt gaaggttatt tcaaagtggt aaaagattta
    30601 ttgaaaccaa aaggaacagc tttgatccat ggaatcagcc gacaacaagg cggtgcgaca
    30661 aatgcttgga tcaataaata tattttccct ggcggttatg tccctggtgt tactgaatta
    30721 gttgataata ttacaaaaaa cgatttgcaa ctgattgatt tggaaagctt acgtcgtgac
    30781 taccaattga cattagaaca ttggacaaag aattttcatc aagtccaaga tcaagtcacg
    30841 aaagaaatgg gtgattcctt ctatcgaatg tgggatctat atttacaagc ttgtgcagct
    30901 tcatttgaat caagtaatat cgatgtgatt caatatttgc ttgttcaacc tggaaacaat
    30961 gacatcccaa tgcatcgtgc ataattgagg caaaagtact gtttaaagac tatttaaaaa
    31021 gaagctaagt tttatgatgc tccagaagtg aacttttttg tctaacttct aggattgtct
    31081 ctaaagctta gtttcttttt ctttatattt tcataataaa agtttggttc tttaattttt
    31141 aaacatgaca gaaaattttt tcttcgttat acttgtaata gaatactatt ttataacaaa
    31201 ggggatcgaa tcaatgaaaa aaactgttat ttatactagt ttatttgcag ctttgttttt
    31261 agctgcttgt caatcaaata ccaatcaagg cacatctggg aatacgtctt ccagtagcga
    31321 acagcgctct tcacaggtat cggactcctc aacaaaagca agcacatctt cgtctagcca
    31381 gacaaaaagt tctagtgaag caagtactca gtcagatatc aaagtgtctg cagctcgtgc
    31441 gatcgaattg ttccaagata aatacccaga tactgcaatt actagtttgg aattagatag
    31501 tgattgggga tcttacttct acaagataga aggtgtcgat gatcaaaaag aatacgaagc
    31561 aagaattgat gctcaaaatg aaaaaatgga gacagagaat ccagaaactc tagatcgaga
    31621 tgaacaaaat ggtcaaaaga aaaaagaaga tggtctggat gttgataaat tgatcacgat
    31681 tgaggaagca gcaaaaatcg ccgtcgacaa agtaggaagc ggtacggcaa cagattggga
    31741 tttggataaa gatttaggta ctacttattg ggaagtcaaa gtaaaagacg gcaatcaaag
    31801 tacatcagtc aagatcaata gccaaacagg cgaagtactg tcaactgaac aagatgatta
    31861 agaatttctt cggaaaacaa gagttgattt ttcttttagt catgatacac tagacaaaat
    31921 aacttgtttg aaggagcgat ttacttgagt ttagaaacga cctttgccaa tcatatcttt
    31981 gccaatcctt tgatgaatgc atctggtgtc cactgcatga ctacccagga attagatgaa
    32041 ctggctcatt cagaagcagg agcatttatc actaagagct gtacgatcaa tgaacgaaaa
    32101 ggaaatcccg aaccacgcta ttttgatgtt cctttaggga gtatcaattc tatgggactt
    32161 ccaaacttag gtttttctta ttatctagag tatgctcttg cgtatgaaaa agtgcaggaa
    32221 aaccaaaatc aaccgttatt tttttccatt gccgggatga gtgtccagga aaacttggaa
    32281 atgcttgaga aaatagaaaa aagtgggttt aatgggatca ctgaattaaa tttatcttgt
    32341 cccaatgtgc ctggaaaacc acaactagct tacgattttg aggccactta tgaaacactg
    32401 aaagaagtat tcagcatctt ttctaagccg ctaggaatca aattgcctcc ttattttgat
    32461 tttgctcatt ttgatcagat ggctgatatc ttgaatcagt ttccattgac ctatgtcaat
    32521 gctatcaaca gtgtgggcaa tggtttgtat atcgatacag aacaagaagc agtagtcatc
    32581 aaaccaaaag aagggttcgg cggcattggc ggagagtata tcaagccgac tgctttggcg
    32641 aatgttcgcg ctttttatac acgtttgaaa ccagaaatcc aaatcattgg tacaggtggg
    32701 attcggacag gacaagatgc atttgaacat ctgctatgtg gcgcaagcat gttacagatt
    32761 ggtaccgagc ttcataaaga agggccggaa attttttcta gaatcataaa agaattaacg
    32821 cagattatgt cagaaaaagg atatacgtca attgacgaat ttaaaggcaa attacgcacc
    32881 atttcatagc aaagagacga aggtcatgat tgatcttcgt ctttttttat aaaaaacact
    32941 cgtctgttca ttcctgtgca agcgtactca ttgtatgtta gacgaacttg tgggaaaata
    33001 acagaggcaa aaaggaggat aatatgaatg atgcaacaga attagcaaaa gagcttaaag
    33061 aaaaagaatt tagttttcaa gaactagtta cggatataca gaaaaaaata aagaagcaaa
    33121 atcatgaatt gaatgcgttt gtcgcgcttg aaccattcga gagtaattcc cacgagctat
    33181 tgattcccca gtcagaaaaa gggccattgg ctggtatccc atttccttta aaaatgctcg
    33241 gtcaagaaaa aaagggatgg ctagctactt caggatctcg tttatttgaa acacaccgag
    33301 cttctcatac atctaattat gtgcggcaag cagaagcgat aggtctagtt ccttttggac
    33361 aaaccaatgc gccagagttt ggtttcaaaa atattacaga cccagtcatc tatggcccag
    33421 caagaaaccc ttggaacctt gaatgtacac cgggcggatc aagtggagga gctgctgctg
    33481 cagtttcatc aggaatcgtt cctttagctg gtgctagtga tggcggtggt tctattagaa
    33541 tacccgcttc gttttgcgga ttgattgggc tgaaaccttc gcgagggacg atgccagttg
    33601 gtccatatgc ttggagagga tggcaaggag ctgctattga tttcggcttg acagtttcta
    33661 tgagagacac ggaggcttta ttttatggga tgagaagtat ccattccggt gcgccatatc
    33721 aagtatcgcc agttgagtgg caaacacatc caagaaaaaa acggttgaag atagcttttt
    33781 gtgttgattc accgatccac tcacagatta gtgaagaggc agtacttgct gtaaaaaaag
    33841 ctgtagattt ccttggaaaa gaagggcatg atttgacaga gattccttac ccactagatg
    33901 gtcgtaaact gatcacgtct tattatcaga tgaatggcgc agaaacagca gcaatgatcc
    33961 gctcgattga aacaggcatc cagcgagctg tcagaaaaga agaacttgaa cccatttctt
    34021 ggactttgtt ccaatatggt cagaagctct ctgccgctac ttacatcgat tctctgcatg
    34081 tatgggatga agcagcaatt gtcatggaaa acctacatca agaatacgat ctattccttt
    34141 caccaacgac tgcatttacc gcccctaaaa tcagcgaaga tttgcaaagt gatgcgataa
    34201 gagcacagat ggaacacatc agtgattgtt ctgaagtaga aggtttagac gttgtttctt
    34261 ccatgtttga ggagagcttg aagatcacac cttatacaca gttggctaat ctgactggac
    34321 aaccagcaat cagcttgcct actcatttga caaaagaaaa catgcctcta ggaatccaat
    34381 ttatggctgc cagaggaaga gaagatttgc tatttcaaat cggctatcta tttgaagcaa
    34441 atcatcagtt ccatttacca aatccatgga aatagcagaa acaaggagaa gaagaatgaa
    34501 gaaaaaaaga ttgtttgctc taataactag tgcctttttt attttaagcg cttgcggtac
    34561 agctgccgct aatcaggaag ataacagctg gagtaatgta gaaaaaaaga aagaattagt
    34621 agtagcgact tctggcacac tgtttcccgc atcttattat aatgatcaaa atgaattagt
    34681 tggttatgat gtagatttgg caaaagaggt ggctaaacgg ttaaatgtca aaatctcctt
    34741 taaagaatac aacgtggatg gacaaatcac ttctgtttca aaaagagaat cagattttgc
    34801 tgcgaacgat tttggtatca caaaagaaag agaaaaaaaa ttctcccttt cagagacttt
    34861 caaatattca tttgacagta tgatcgtacg aaaaaaagat gattcaggaa tccactcact
    34921 tgaagatctt aaagggaaaa aagcagctgg cgaacccaat actagctata tgcgtttagc
    34981 ggaaaaacac ggagctaaat tagtcacata tgacaatgcc acgaatgatc agtatttaag
    35041 cgatgtagca aatggtcgaa cagatgttat tctaaatgat tattatctgc agaaaatggc
    35101 cgtatctgct ttaccagata tccctgtaaa aatactggag aacgtttatt tcaatgaaaa
    35161 cgaaagtggt tttttattga aagaaggaaa tgatgctctg aaaaagaaaa tcgatggcgt
    35221 attaaaagag atgaaagaag atgggacgac aaagaaaata tctgaaaaat attttcaaac
    35281 tgatgtttca gtcaaaccga aagaaacgat ggctgagtcg gtgagtgagt gatgtacttt
    35341 cctctaattg atttttccct catgtggact tcacttcctt tcgtactgca aggcttggta
    35401 tacacattag ggattggctt cgtgtcgttt cttttaggaa atctggtagg gttgcttttg
    35461 acagttttag gtcttttgga ttggctgcct ctgaatgtat ttattcgttt ttatttatcg
    35521 ttctttagag gcattcccgc actagtcctg ctgtttctgt tatattttgg gttaccttat
    35581 caattaagtg ccttaacggc ttcagtcatc tgctttacga ttaccagcag tgcatttatt
    35641 ggtgaaatct atcgaggatc actagcaggc gtttcttcag gtcagtggga cgctggttta
    35701 gcttctggtt tttctttctt tggggtattg aaatatatcg ttttgccgca agcttttcga
    35761 atctcagtac cggcactcag taacgtagct atggatctag tgaaaggtac ttcgttagca
    35821 gcaacaatca cagtgccaga aatatttcaa aaagcaaaga tcataggcgg tcggacattc
    35881 gattattttt ctctctatat tttagttgca ctgatttatt gggtcatttg tatcgggatc
    35941 gaaaaagtgc aacagtattt agaaaaacgt tctatgcatc agtcaatctg aaaaatagaa
    36001 atggtgctag cgtggctggc accatttcta ttttttgttt cttgtatatt ctgcctagta
    36061 ttttaaaacc atttcatgta aaatgtaaat cgaaaatatg ttcggaggta gaaaacatga
    36121 agaccccgct aaccatcgaa atagaatcct cgttatacca atattgcatc gagcaaggcg
    36181 gcttggttgt agaagaagtc acgatgcctg atgaccaagg aattgtagat acgcttagct
    36241 gcttgacaaa atttgatgga agccgagaat ggcgatgcta tgagctgaaa gtgacaaaag
    36301 cagattttcg ctctacagcc aaactgtcat ttgttggaca ttataattat tttgttttac
    36361 caagtaaact atatgaagaa gtaaaaactg aaatccctac aggaataggt gttttagtgt
    36421 atcgtccgta cacggttgaa gaggagatgc ctgcctctgg tacatttctg attgcgaaaa
    36481 aagcagcaaa gaaagagctt gctgtaccgg aagaagcttt gacaaaccgc ttcatggctt
    36541 ctttattcag ggaagtaaga aaagcaaagc aaatggagcg aggcgtaaaa tattttccaa
    36601 cagatcaatt gtacaaagaa ttgaaaagac gttcagaaaa tcaagatcca ttttctaacg
    36661 aacgatatta tgaacggttt ttagaggaaa tagctagtca ggcaatcaat gagttgcaag
    36721 aagaagttat cgcgcttcag catgattatg aatatttgag gcaacaacag cagatccgtc
    36781 ggttgcctac agagccattg gagtgaaaaa tatgtactat ccttatttaa gaggaaaaca
    36841 atttgaccta ttagcattaa aagaagcact gtcgcgagga ttgctctcca ataaaatcca
    36901 accagtgatc gaaccagtac gtgattccgc tacgcttaaa aatgtgatcg aattatttca
    36961 aaaaaagcac catccaatcg ctgtgatcca aaatccccaa gtaggacaat tcaaattatt
    37021 cgatcagcat gtgcacagtt ggacagtcaa agaaaatagc tctgtcgtcc ctgcacagat
    37081 cttaacgcca gaaaatctca gtgaagtact ggattcacct ccagcgtttc ttgttttcga
    37141 tggacagcac tacccaaaag atacagaggt gtggaaacag ttggcaggag ttgattcgaa
    37201 atttttgata ccagatacta gtcgctttcg catctggctg cccgaaaaca aaattgtgat
    37261 ccgtgactct tttcaaacga gaaagcatgt tgaaagttat gcagataaaa cagatgactt
    37321 ttttagtgat gactatcttt ttttccacgc agacggatat attggatttt ctgatttcac
    37381 gatcgaaggt agccgttatt tcgataaagg gttccctagc cgagcgcttg ctatccatct
    37441 aacgtatatt gatgcttacg gcaatatacg cgtgaaacat tttgtctctg acagtaacga
    37501 ctctgctaaa gaccaagcat tgaagtttct tgaagcagga gataaaatga aaaaatggat
    37561 catgcgtcac catcatcaac tcttgattac ttcaggaatg attgaattat tggctcttta
    37621 ccagcaacaa aaattccctg gactgggagt gttgaaaaag tggtcactta tgcatcattt
    37681 ggaacttatt agtcacctgc tagatcatcc aaaagattgg cttaaatatt attcaaaagt
    37741 tcaggaacta tatgaagtga tccaatgatt agataatgta aatgaaaaag gaatagaaaa
    37801 gaggaaactg gatgtatatt cggcaaacaa ggaaagaaga agtagaagaa gtggtagaaa
    37861 tcattaatgc cgcagcggat ttcattgctt cgcaaggctc tcctcaatgg caaaacgggc
    37921 agaaaccaac aaaagaaacg attttgaacg atattgaaag aaaagaaagc tatgtattaa
    37981 tagtggaaga tcaaattgct ggaacagctg cattagtctc aagcgtggat cccgtttaca
    38041 caaaaataga tggtagttgg aaagaatcaa ctggtcctta tttatcgatt catcgtgtcg
    38101 ctttagcaga aagtactcga ggaaaaaaac tagcaaaacc gttcttagaa catttgttga
    38161 cgattgcagc cagcaaaaat atccaagata tccgtatcga tacttatcca gctaatgaac
    38221 cgatggaaaa gaccatctat tctgctggat tcacttatca agggatgatt gaatttcctt
    38281 ttgaacacgg cgaaagaaaa gcatatcaaa taatcatcaa ttgactataa aatcatctga
    38341 gcaaagataa agacattaac actactgaaa aaaacaatca tatgatagcc aagaggttgt
    38401 aacagaagtg gccagcttca aaaaacaaga aagaatttcc gaaaatgctt ctcaaatttt
    38461 tgtggaattt tagcgtattt tacgaaatag ctgctttctg aaactccctt catcgagagg
    38521 tcgtgacata gcttttgtca cgacctctcg gcttatccta cgagtattat tcgatacatt
    38581 tatatcaata atcctttttt tattttatct gcagcttctt ggaacgctcc atcgtttgac
    38641 acgactaaaa gtgaggggat agctttcaaa cgtttcaact gtgctaaatc ttctacataa
    38701 accgccagtc tggctgaaat ctgttcagat gaatctccac gttttttcag gcgcgattgc
    38761 aatgtttctt tagagatcgt aataaagata ggacggacta ccgtcccgaa atactcatat
    38821 aaatgccaaa aaccatttgc tgtcaaaacg gcgtaacaat ttcctactgt cagtttcttt
    38881 ttaatttctg ctttactgga accatattta tgttgagcat attggtccca ctctgcaaac
    38941 tcatttccct tgatcatctg attgaatgta ttgtcagaaa caaaataata atcctcttga
    39001 tctttttccc caggtcttgg tgatcgattc gtgtaagtaa tgatcttttg ttccggctga
    39061 aaaatagctt ctgcgagtga agtttttcca gaaccagaag gaccgataaa tacatagcaa
    39121 taattcattg tcatttctcc tttgatgatt gaacaagtga aaaagctttg gaaaatattt
    39181 ccaaagcttt taaaagtaac tttgcttttt tgtatattat accagcaagc cagtcacaat
    39241 agcacttaaa aagctgacta aagtcgctgt tagcaaaatt ttcaatccgt gttttgcgac
    39301 aagatttcct ttttcttcat ttaatccctt catcgcacca gaaatgatcc cgatagaaga
    39361 gaagttggca aatgaaacga ggaagacaga tataattgct gttgtacgct cagtaaaatg
    39421 gaaattacca tttgctaatt ctgtcatagc aacaaattca tttgtaacca gtttagtcgc
    39481 catgatacta ccggcatcca ctgcttcttt ccatgggatt ccgctgatga aggcaagagg
    39541 ggcaaagatg taacctagga tctcttggaa agtgatgccg aatatcccgt ggaagatagc
    39601 attgatcatg gcaatcaaag caacaaaacc gataagcata gcagcaaccg tgatcgccac
    39661 atggaaacca tcaagaatgt attctcccaa catttggaaa aatgtctgtt ctttgttttc
    39721 ttcaatgatc agctcatctt ctttttcttc taatacatag ggatttacaa ttgaagccag
    39781 aatataaccg ccaaataagt tcaaaacaag ggcagtgatc acatatttag aatcgatcag
    39841 tgccatataa gatccgacga tcgacataga aactgtagac atagccgata cactcatggt
    39901 gaacaaacgt ttctcagata ggaagggtaa ttcctttttt atggaaataa aaacttccga
    39961 ttgtcctaaa atagcagaag ctactccatt ataagattcc aatttgccca acccgttgat
    40021 tttacttaat cctagaccaa cagctttcat aaataatgga agaatcttga tatagcgtag
    40081 aataccgata atagcagaaa taaatacaat tggcatcaat acggaaagaa agaaaggtgc
    40141 acttccttta tttgctacac cggcaaaaac aaagtcaaca ccttgcccag caaaagcaag
    40201 aagatgatca aacatttttg ccaatacaga aacaaccgct gtaccaaaag ttgtacgtaa
    40261 taatacgaaa ccaagaacaa gctgtaaaac gaacatcaca attagtggtt tcaatttgat
    40321 tgcttttttg ttactgctga tcaaaaaagc aagccctaat gtgacaaata aaccaataat
    40381 acctaataaa taactcaaat aatcactcct attccaaatt ccgttttatt ttacctgttt
    40441 tgcaaataaa atactaatct ttagtgaaat tttcattact tttttagagt gaaacgcatt
    40501 cattttccat tagaaaataa agcgaacaaa ataaaaggtt taaaaagtgt aacgtcttag
    40561 taaagtctat aaaaaaacga aactatcgat taagctaata gtttcgtttt caacatttat
    40621 aagtaaactt tttctattgg taatatagat tttcggaagg gtagacaggt gcgcaggtaa
    40681 tatcgattcc tagttttttc aatgtttgtt catcattttt attgaccatc acagtagagt
    40741 gggcttgtgc tccctcaagt tcagctagtt tatcataagc aacttgagcg attggattcg
    40801 ttactgcact gattgataaa gcgatcaaga cttcattgat gcttagtgca tccaaagagg
    40861 aatgaagtgt ctttgatttc aatccttgta ttgtttcaag aactaatggt gacaataaag
    40921 gaatgtcatc tgcgatatgg gctaaatgtt ttactgcatt taagataact gccgctgacg
    40981 catccatcaa tgaacttgta cgcccggtaa tgatgacttc atctggtaat tcgattgcaa
    41041 tcacagcaga aggatctttt gtaggacttt gcgcttgaat cttatctgca tagtcatggg
    41101 cagggactac cacgtttcgg tcttcaggac gtaaaccgac ttcttccata atcagtttga
    41161 ttcgattaac tgcttcttcg tcgatcaatc cttttttgaa gtcgcattct gtttggaaat
    41221 aacgacggat gatttcttgt ttagaggctt cttggacaac ggtatcatcc acgatcccaa
    41281 atcctacacg attcacaccc atgtcagtag gagattgata aacagattct ttaccagtaa
    41341 ttttttcaat aatacgtttg attacaggaa atgtctcaac atcccgatta tagttcacag
    41401 ctactttatt gtaggcatca aagtggaagg aatcgatcat attgacatct tttagatcga
    41461 ctgtggctgc ttcataagca atgttcagcg gatgtttcaa cggaacattc catactggga
    41521 aagtttcgaa cttggagtaa ccagctgcat gtcctttttg gctttcatga tacagttggt
    41581 tcaaggaagt agcgagcttt ccacttcctg ggccaggagc tgtcactaca acgattggtt
    41641 tagttgtttc aatatactca ttttttccaa aaccatcttc tgagacgatt ttttctacgt
    41701 tgactggata gtcttcgatt tctgagtgtt tatagacctt gatgtttcgt ctttctagtt
    41761 tattgataaa gactttcgtg gcaggctgtc cactatagcg agtgataacg acactgttga
    41821 ttaataagcc ataaccacgt aattcatcga ttaaccgcaa gatatccata tcataagtaa
    41881 tcccataatc accacggatc ttattacgtt caatatctcc agcatagacg caaatcagta
    41941 tttcagcttg atctttcaat ttttgcagca atttgatttt cgcatcttct tcaaatcctg
    42001 gaagtacacg tttcgcatgc ttgtcgtcaa ctagcttccc accaaattct aaatataatt
    42061 tgtcatagtg gttcactcgt tccaaaatat acgctgactg ctcttcaata tatttttctg
    42121 aatcaaaacc aatttttttc actaggttcc ctccttaagt actaaagcat tttttcattt
    42181 ttttgaggat tcgtcaactg gaagagcgaa attcctcaaa aaaacaaaga aaatggttta
    42241 aaccaacttg tctagatagt tattcattgg tatgatagtt tttgataagg aaggtgctac
    42301 atgaaaaaaa gacagaaaaa gaaaaacgca tataaacact atatccgttc gatttttacc
    42361 ggttatgaaa gaatgcttga agatcccgaa ttggaacaat tgacgtttac ttatctaaac
    42421 gaagaaacgc agctaactcg tgatgagcat cagcgcattc atttcacaac aagagatctg
    42481 cctagtaaat aaaaaataag ataaaggtca tcggattcga ttctgtccta tcagatggaa
    42541 agactagttg atcgaaaaaa aggctgtacc aaattttggt gcagcctgtt ttatggagca
    42601 ttgcccgctc ttttttataa caatttttta cagaaaatgg tactattgaa gtattaataa
    42661 gtgaaaaaca gatgagtaag aggaggcaag tagtatgaaa gagccagata caaatgcaaa
    42721 ttatgagcca gacgaactag aggagacgaa tgcggatcag gatacaggac aagaatcgtc
    42781 ttctttttac gatatcaaag aaaaagcaag catgaatctt tcccaattat tagaagactt
    42841 acaggctttt gaaaaagctg tccgcgaaga agatatccct gagatctacc gtttatacaa
    42901 tggccgattg aatgaagaat taaaaagaag ctcaaatgaa aatcacgaga tcgatcaatt
    42961 gctgatgcgt aagatccatg atcgtttttt acaggaattt ccttttatgg aacaagtaga
    43021 aaacatctcg ccaacgatga gctattacaa aatcggcact tattaccatg atcggcccac
    43081 ggtcggtatt gatgcaagtc tgccagaaat ttttgttttg cctaagatcg atgaagagtg
    43141 ggagaaatat tcccaaccgg agtcaacgga ctatgacaaa aaaatcaatg agctagacgc
    43201 aaaggtgatc actgctcaga cagaaattga acgtttagat gaacaaataa aagaaatcaa
    43261 tcgacaaatg actgatttga acgacaataa aggattcttg aatcgtaaaa aaatcgagga
    43321 agaaattcaa gaattagaga agaagaagca agtactatct aatgaaaaat taggctggct
    43381 tccttatatt gagacgcctg aaacaatcca gcagcaaaaa gaagcattga agcaagaagc
    43441 aagagcagac cagcttcgtg cagccatcgt agaaaaagaa caaaggcaaa tcagacgcta
    43501 tttcggtagc aaagagggat tcggtcaagc aatccatgaa ttcttgatga attatcttgg
    43561 caatgagaat cctaataatc aatctaatga aggaggttcc gagtatgaat gaacacgatg
    43621 aagcagaatg gacaaaaaat gaagaacaaa cagaagatca tgaagaaacg aacacttcat
    43681 tggaagaaca aacaagtaat cctctggatg acacagaaga aatccctatg ctagaggaaa
    43741 caaaaataca cgaaaaagaa aaagtaactc cgacgaatac gaatactgca gaaaacaccc
    43801 atgcttacga aataactgaa aatcccgggt atttagaagg aaaagagcaa gatcatcaag
    43861 atatcgtcag tttgattgaa acagcggaag cagaattagc ggatttacgt atgagaaaag
    43921 atttgatcga atcagatttt ccacaattat tagaatcata tgatcgtttt atattgaatg
    43981 aagaaccaat ctccacgatc ggaaagacaa gcctgatcga atacttcacg tatgaattac
    44041 tgaaaccgtt gaatgatatt ggtctggaac ggtcaaattc agcttatgac acatggaaaa
    44101 caagacattt ttcgatcaat tatacgttga atggacaaaa cgaattagtc ttttcctttg
    44161 tattaccgac catcgatcaa cgataccaag tggaatcgat gagtctccta aacgtatcac
    44221 cagagacaat ggaaatcaat gtccaagatg accgggtgct ttctttgatc cgacactggt
    44281 cagtggatcg agtattctct gcagggcaga tcacgatatt caatcacaaa ttgaatcaat
    44341 tattagccca tgcaagaaca cttggtttta ccgttaatca gacattacta gacaatacga
    44401 agccacttcg tttgaatctt cagagcgaat ttgaattgac gaatgaagta ttggatgata
    44461 tttttatcgt ggcaatgaaa aatccatcct acgatatgga aaagatcaac gaaactactt
    44521 atcaagtacg attagataaa ggacagtctt taacgataac aacaaacgaa aaagatcaaa
    44581 cagtcttgtc gatctcatct ggagactacc cacgatctgt cattgatttc tttatcaatt
    44641 acgaattctt ggtcccatta atggtgagaa agtcctgatt ggataagttg gattaccaga
    44701 tgtcattcag ataattctgt tccaaaggac ttgcggcagt tatccagaat aattgtcaca
    44761 agtcttttct gtatgtacta aatggacgtg aataacttgc tgatgaagga gggatttcat
    44821 taggaatgtc cacaaataaa atagcacagc ttagcttatt gtctgctgct tgtgtagcag
    44881 gtaggatcgc ttttcaattt attccgaatt tccagccaat gactgctatt tttttattta
    44941 tagtacttta cttaagctta aaagatgcgt taattgtcat gagtctttct attgcgatca
    45001 gcagttttta ttttggtgtt ggaccatggg tcatcggcca atggatcagc tatactgttg
    45061 ttctgagttt gttttcaatc gtctgcttga gaaaaacagt gcaaaggaat ctatggttaa
    45121 agggagtctg tttttttctg gcgggcatgg tttacggaat aggtatgacc gtttttgata
    45181 cgctgttata tcgtttgcct caaccttgga tctattacct tcaaggggtg tcgtttgatt
    45241 tgatgcacag tataggcaat gttgtgttct ttcttgtttt tctgccagtc gtcaaacgtt
    45301 tttacaatca ttcaggagga aaaaatgaaa aaaataattt atagtttgct tgcaatgagt
    45361 gtcagtatac tcattttgac aagttgcagt acaacctata cacaatcggc ttccaaccaa
    45421 aaggaggaag taaaaacatc gcaaaaagca acaatcacgt tacaagaaaa tggtcaaaac
    45481 ttttcggaaa aagaagtgtt cttcaaaaaa ggcgatgatt tactgagtat tttgaaacga
    45541 aattttgaag tcaaagaaga taacggcttc atcacttcgc ttgaagggca ctcacaggat
    45601 gagaaaaatc aactctattg gatgtttact gtggacggga aaagtgctac tactggtgcc
    45661 aaagagatca aattgaatga tggacaatcg gtcattttca atttgtctaa actataagtt
    45721 tcataaaaaa ctgttaaagg gcgaatattg ccataattca gtgagttcag ttgatgaaag
    45781 aaggaaaata acatggaaaa ttttaaaaaa agcggaatgt tttccgtaat tgctgctcta
    45841 ggggctaata ttctagttgc tatcagcaaa tttgtcggct atgcaatcag tggcagtgcc
    45901 gccatgctaa acgaaagcat ccacagtatc gttgattgca gcaatcagat tttgctgtta
    45961 ttcggtgata aacaagccaa taaaggacag agcgagttgc atcaatttgg tgaaggacga
    46021 gcaaaatact ttttcagtac aatcgtcgca atgatgcttt tcttcggcgg tggggcactt
    46081 ggggtgatgg aagcttttga aaagttgacg catccatccc atgaagtggg taatccgatc
    46141 attgttatag caatactgtt atttggtttg gtaatcgaag gaagttcgtt acgcgtggca
    46201 atgaaagaaa tcaaagaatt gaatactgaa aaactttcca cgatgcgctt tttgcgcgaa
    46261 agtcgacaca gtgaaatact gattattttt acggaagact tgtgtgctgt aatcggtttg
    46321 gtcttggctt tagcaggaac aatattgact tatgcaaccg ggattgctgc atttgatgcg
    46381 atcagcggat tgctgatcgg tttcttgctg atgggagccg ccattttcct agcaaaagaa
    46441 ttttatagtc tgatcatcgg tgaaagcgtg acagcaagcg atttatcaaa aatcaagtta
    46501 gcttttgaac gtcctgagat cagtcggctg atcgatataa aaacagttca tttaagtcca
    46561 acggagattt tagttgcagc aaaagcagat atcgttggta acaaagagaa tgaagcttac
    46621 tcggtcatca atgatattga aaaaaatatg cgtcagtctc ttcctgacaa gaagatgtat
    46681 atatacattg aaacagataa atatgaccca aattattctc gtaaataaaa cgtttattca
    46741 tgaattgaag caaaaatgag aaaaatataa tcttgttttt aaataaaacg gctacaattt
    46801 agcgaattta tgttacaatg agtccgctgt ttatttgttg taattaaagg agatatttta
    46861 atgaacaaaa agaaaagtgc gccatggctt caagctttgc atgcagtgat gccactttgt
    46921 ataagctata taccagtagg attagcttgc ggagtattgt tgcaaaaagt tggatttgat
    46981 cctctcttat caggactgtt atcgattctg gtcttttccg gtggcgcgca gtttttagtt
    47041 gcatctatgc ttacgacgca agcatcattt gcaacgacat tattgatggt tttcttttta
    47101 gaattacgct atacattatt aggagcaagt cttgccggtt ttatgaaaaa agaaaagcgg
    47161 acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa actatgcggt gaactactta
    47221 aagttttcaa ctgacccttc ctggaataaa agaaaggctt tatatgtcaa ttggttctca
    47281 atgactgcat gggccggtag caatatgatc ggtaattttt tcggctcggt gattcgagta
    47341 gatgctgacc ttgtccattt tgctttgacg gccatgttca ttttcatgtt tatcatgcag
    47401 atgaaaagcg cactactgat ctttacaggt ttattctcag gcgtattagg ggtcgtattc
    47461 atggtattat tccaaaatac tttcggattg attgcagcaa cagtcattgc ttcatttgcc
    47521 ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta agaaactaag agaccgcggt
    47581 aaagatgtca atgagccgct cttcaacttc ccggaacagg aggccaatca ccatgactaa
    47641 actatatttg ctacttttag ttgcttgtct gttcatcgtg gcatatatcc cgcgcttgtt
    47701 cccgatgctt tattttactc atcgaaaagt gccatcctgg ttcagtgact ggatgaaata
    47761 tgtaccggtg gcattatttg cggcgttagc ttttaaagat gttttcatta ctcatgaaca
    47821 tctggatatt gcttggaata tcaaaatagc tgcaatgatt ctagtagcag gcgtggctta
    47881 caaaacacgt tcgatggcgt tgtcagtttt gacaggactt gcgtctgtct ttttgctgtc
    47941 tatgctttaa actgttttat tttattaaaa acagagattg cgacataagt tgttcaaact
    48001 gatctatccg aactgcattt gcaacagtca attctggatg caagtttatt cgtagggata
    48061 aaaaagatta gtgttggctt atgatgcaag ctctttttta ttttaggaat tagagaggga
    48121 tttgtccttc tatgattttt tgctataata gggaagatgt ttattttatg aaagaatagt
    48181 tgagggaatt taaatgaata aaaaattgat gcaaggaaca ttttggctaa cctttgctaa
    48241 tcttctatgt aaagttttag gggtagtcta tttgattccc tggctaagta tgatggggaa
    48301 caatcaagat ggtatgcttg ctacgacatt gtataatgtt ggctatcttc cttatggatt
    48361 gttcctgatg cttggtacag tcggatttcc aaatgcgatt gcaaaaaaag tagctgtagc
    48421 cacaaaagaa ggagataacg ctgcttgtcg gctgattttt agaagtacga tcaatatcat
    48481 gtttgtcatc ggtatcgcct ctgctgcact gatgtattta tttgctcctt tactagccgg
    48541 aatcagtccg attgctaacg ttaataatgg gatactagct atcagaagtt tatgcccatc
    48601 tttgattgct atcccaattc ttagtgcaat gagaggatat ttccaaggaa aaaatgatct
    48661 gcggccttat ggaacttctt tgattatcga acaggctgtg cgagtgatcg ttatcttggc
    48721 tggtacttat tacttgcgcg tattgactga cggaaccata ttagaagctg tattgatcag
    48781 tacagtcgct tctttcttcg gtgggttagc tgcgattatc catatgtata tcgtcggacg
    48841 cagaaaagac ttcttcgaac ttaaagattt tttgatttcc agccgttatt tcgaaagaga
    48901 aaaccgatct gcctctgtct ctatcattcg ggaaacacta ccgtttattt ttgtcggctc
    48961 agtcattact atcgttcaaa tgatcgacca agtaacgatg aaaccactgc ttcacttttt
    49021 ccgtccagag atcgcagatc agcaactgga attcttattc agccgtgctt ctgtcaatcc
    49081 aaataaattg acattgatcc tgatctccat ggtaggaaca gtagctataa gcagcttgcc
    49141 aatattaagc acgatgaaga aaaaagatcg gatacaaatc gaaaaaacag taggagatag
    49201 tttttcgatt gcgttattga ttcttcttcc ttcattgact ggaatgtctt tattagctgg
    49261 accactatac acattgtttt ttggctatga tcctgaaagt gtcggatatt tccaaatggc
    49321 tttgctagct tctttgtttt tctctttatt cacgatccta tctacgatgc ttcaatcttt
    49381 gaatcaccat cgttttgcga tccgattaac tgtagaagca attatcctga aagtcatctt
    49441 ccaagtaatc ggattaggat tagttggcgg atacgggatg agcttatcaa atggcttagc
    49501 attcggtatt gtctttgttc gcggctatca ctttatgtgc aaagaatacc gaatagcacc
    49561 tttagcaaag atcagcaatt ttttcttaaa aactttccgc tccaccctga ttatgcttgc
    49621 agtatgtttt gccgttttct ttttgctgaa tcagcgattg tcaatggaat caaaaagcta
    49681 tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc ttagtatttt tattttctca
    49741 attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa catcatagca gaagcaaaga
    49801 ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg gcaactcgta tgattacgct
    49861 tgtcgttttt ctattttcca attttagttt catatctaat cttacttatg atatagttat
    49921 aaggtctgga aaaattcttt gaaacgagga aattaaacac atgaaacttg cagtagttac
    49981 agatagttcg gcatatctgc cggaacgtat caagaaccat cccgacttat tcgttattcc
    50041 aatccctttg attatggatg ggaaaattta taacgaagga atcgatatcg aagcggatga
    50101 atattatgga cttttaaaaa acagtaaaga ttttcctact acttcacaac ctgtcgtagg
    50161 ggaagttttg ggtttatatg aggaattgaa agagaaaggg tatgacacga ttttaagtat
    50221 ccatctttct tcagggattt ctggctttgt caacacatta tttgcgatga aagatgacat
    50281 cagcggagta acggtcattc cttatgactc taagatcact agtatgccga tgggacacat
    50341 ggtagaagct gccttagact taaatgatga aggtcagtcc cttgatgaaa tacttgcaca
    50401 tatcgatcga atccgagata atacctacgc ttatctgatt gtagatgatt tgaacaattt
    50461 agtacgagga ggacgtttga caaatggtgc agcgctgatt ggcagcttat taaaaatcaa
    50521 accgatcctg acattttctg aagggaaaat tgttttattc gaaaaaatcc gttcaagtaa
    50581 aaaagcattt gctcgagctg aagaaattat tggtagaaga aataacgaaa tacaaccgcc
    50641 agtcaaactt tacgtgatac atgcaaataa tttagaagta gcgattgcgg agaaagagaa
    50701 attgcagaaa aaatatccaa atgcagaaat cgaaattggt catttcggac cagtcattgg
    50761 tacacattta ggtgaaaaag ccatcggcat tgccatttca gcgcaataat tccttttatt
    50821 caaaaaacat accaaaaata ttccaacatc atcttggact ttctacatgt tactaatgag
    50881 cgtgtaaaag aacgagatgg tgtttttatt tctatcaatg aagctctttt tctttgcaat
    50941 cctcacttca atcctctata atgtcgcata tagtttagta aatgagtaaa ctactaattt
    51001 acttatttac taaaaataaa aggatgagga gttaaaataa tggaaatacc aacaaattta
    51061 aaacataaac cagtaatcgt tgtagaagac tatgataaag ttgatggaag aaatgcatta
    51121 aatacggatg caaaagggct ttcactgggc cttgctcagt ggaatgatcg tggaaaagtc
    51181 gatatctcag gcaaggtatg gcgacatact ggagaaaaat ggtcacgaca gtcggaagaa
    51241 ttaccaatca cacgtatttt agatttagcg atcttagctg cacaaggaag tttatacttc
    51301 caagatgctt atcgttatga aaaattttat gatccagaaa atccagtcgt agacatcatt
    51361 ggattacaag ggaaccgaat gacggtagaa gtagatacca aaaatccaaa gatcgatgaa
    51421 gacatcattt tgtattacga tacactacaa aaagacggag aactgattgg ggaacgattc
    51481 cgtattttaa aacgtctttt agatgagcta ggatattaat agacagagat gatcaaaagg
    51541 ggaagagaag catggagaat ttctcactaa cagaaaaccg aaccttttca cggctagaaa
    51601 agcaaatgat ctggaagaaa ccgaaaagcc atcggacaag tgcagaagaa tggcggatcg
    51661 cctccgaaat caaagggaat tggcaagatc ctgaaatgaa aatcagtaac gtacttttgg
    51721 aaggagatgc cgggtcgggg aagacacaat tagccaaagc actatctgct gatctgcaac
    51781 ttccctatac aaaagtgacc tgttttgcag atatggacaa atcagatgtt ttcggagcct
    51841 tattacctgt agtagattct aataaggaac aagaccaaga attactagaa gctatttacc
    51901 aaacagatac acttgaagct gtactgacac tgatccagaa ccattacggc attgatcctt
    51961 tttctgctaa agaaaaatta gcagagctag ttcaaaggat cgaagctgat tatccagatg
    52021 caatccatta cagctattac ccttctgaaa tcgttcgagc gattgaaaaa ggatatttat
    52081 tagagattca agagccgact gtcatccgag atgcctctgt tcttgtggca cttaactcag
    52141 cgctcgaacc aaatgggatg ttgaatttac cgacaggaat cattcgtcgc catccagatt
    52201 gcgtcattgt gatcactaca aatcgaaatt accaaggaaa tcgcccatta aatgaatctt
    52261 taagagatcg gatgcaacat gcggaaaaaa tggatcttcc tgccttagaa gtcatggcag
    52321 aacgtgcgat ggcgaaaact atgatccaaa aacaagaact tctcttaaag atggcagaga
    52381 ttatccgttt gttggatgaa actgcaaaag ctaacgccat caaaggtgta gcaggaatgc
    52441 gttcttattt ttattgggca aatacctata agcaaggaca agatttactc cagtcgattt
    52501 atccaaaagt tttgtataaa ttatccactg attcagatga attggcaatt cttgaacaag
    52561 ctttgtctga cagtggattg ctactggaac ttcaggacat attgagaaaa gaaaaatggg
    52621 gtcatcctca atctgagcat acaaaaggaa gaaccattag cgccgaagaa gcaaatgaac
    52681 gaaacatcac tgaaaatgct gaagaaattc tgcgcttaga tgaaaagaaa tcgttgtctg
    52741 ctgaagaacc aaatgaggga gaagagaaac aagaagaaac aatagaaaaa aacgagattc
    52801 ctcctcaaat gcaaaaagaa cgcagtagcg atatcgaagg aaaaaatcaa aaagaacaac
    52861 aaacaggaga tgattctcaa gaaatgtctg cttctgacat ggaagcacat gataaggaaa
    52921 tcaagaagca actgaataaa gaagcgcgac aaatcatgaa ggaaacgatt catgaaaaag
    52981 aaggtctcat cgttcaccga ccagctttcc aaggaaaaac gccggaagca cgtgagctac
    53041 acatgcaagt aatgccaata gtagaatcac ttagccggca aattttagag ttactggata
    53101 atgaacaaac agagacgtac caaaaaggaa aatacgaagg tcagcggttc aatgcaagca
    53161 aagtagcata tggtgatttg agaaattttg acaaaaagaa cccgccacat gagcaacctt
    53221 ctttagcggt agctgtacgg atcgatgaat ctgggtcaat gatacgggat gatcggatcg
    53281 aagcggcaaa aaaagcagcg attgccattg ccgaattttc taagaaagtc gatatcccat
    53341 tgttgattta cggagataca gcagaccgtt cgtcaagaga aaagacttct ttgttttctt
    53401 ataaagaatt tgaagacgga tttcaatggt taaatgaaaa attagtcact atgaaaccaa
    53461 ggcaaaacaa tcgtgatggt gctgcacttc gtttagcagc agaaaaatta aacagacaat
    53521 ccgcaacaac caaactgttg cttaatatta gtgacggtca gccaaaagcg ttgccggatt
    53581 atacaggcaa aaaagcaaaa gaagatatcc agtctgtttt gaaagaatat gaacgtcaag
    53641 gaatcctatt tgtctccgca gctattggcc aagacaaaga agaaatcaag tcgatctacg
    53701 gtgaaagtcg ttttgtagat attactgact tgaatgaatt tcccaagcaa atgattcaac
    53761 tgattgcccg ctatctatga taaaataaag ctcaacgaac gcacttagca gattcgttga
    53821 gcttttcgtt tctagttacc ggtaacaagg atacttctaa aatagaatcg gttataatca
    53881 agagggaccg ttaaaggagt gaaacgatat gatacaagaa caaaaatggt ggaaagaagc
    53941 agtaggctat caaatctatc ctgctagttt caaagatagc aatggcgatg gtcaaggaga
    54001 catcaatggg atccgtgaaa aacttaatta tttgaaagat cttggtatcg gctttatctg
    54061 gatcaatccg atctatcaat ctccatttgt cgataatggt tatgacgtaa gtgactatca
    54121 agatatagaa gagagattcg gaacgatgga agattttgat ttattgctaa aagaagcaca
    54181 tgaattaggg atcaaagtga ttatggatct tgtcatcaac catagttcgg atcagcatca
    54241 atggtttgaa gaatcgaaaa aatctaaaga aagcccgtat cgcgattatt acatttgggt
    54301 cgacggggta gatggcaaag aaccgaataa ttggacatcg attttcggcg gttctgcttg
    54361 ggagtattct catgaaactg gacagtatta tctgcatgtt tttgcaaaag aacaaccaga
    54421 cttaaattgg gaaagcgaga aattgaaaga agaactattc aatatgatcc gatggtggtt
    54481 ggataaaggg atagatggat ttcgtttaga tgcgatttct catgtaaaaa aagatgaata
    54541 ttcagtaaaa gccacagaga accctttctc accctttcaa aatgtctcag gaatcgaaga
    54601 acatttgacc gatttgaaac atgtttttga agagtacgat atcatgactg tcggagaagc
    54661 aagtggtgtc actgcagaag aaggaccaca atgggtaggg aaagacggtt atttcgatat
    54721 gatattcgaa tttgatcata tccatatctg gcagcaagaa aaagaagggc aattagatgt
    54781 ccttaaattg aaacttgcat taagcgcttg gcaaacttca ttggatggta tcggatggaa
    54841 cgctttatat atggaaaacc atgatgttcc tcgtgccgtt tcagtgtttg gagatactcg
    54901 tcccgatttt tgggctatgt ctgcaaaagc aatcgctatg atgtatttct ttttgcaagg
    54961 tacgccattt atttatcaag gacaggaaat cggaatgacg aatatgccct ttgaatcgat
    55021 cgatcaagtt gatgcagtag attcgaaaag actttataaa aggctgcttg cggaaggtaa
    55081 gacaagagaa gaagcattgg atatcattcg tgaaacaacg agagacaaca gcaggacacc
    55141 catgcaatgg acttcggaac aatatgctgg tttttctaca catgaacctt ggctgatcac
    55201 taatccaaat accaaaacga tcaatgtaga acaacaagaa tatgaaccag agtcggtcct
    55261 gcaattctat aagaacatga tccgcattag acaaaccaat aaaggtctga tatacggttc
    55321 ttataaagaa tatcttcatg aacatccaca actttatgtc tatgaaagat atttagaaga
    55381 tgaacaatat cttattatgg taaacttaac agaatcactt gctgactatg aacttccaaa
    55441 agaagcagat cagtcctgga cattactctt gtcaaattcc tctagcggag aatttgaagc
    55501 taaaggcata ctagcacctt atgaagctcg gttatataaa acaaacaagt aaaacttcct
    55561 cgttaaagtt cattagttgg actttgcgag gaagtttttc agattgctct gtcaattact
    55621 catttcaata attcgctcta ggaccaccga tatatttagg acgagagcga agggctgtca
    55681 cgtgagcttg tccaagggtt ttcaaacgag gtctgacagg tacttggaca tgtttgatat
    55741 gcataccgat tgatgtgtca ccgatatcga taccggcttg tccaactacg tgttccactt
    55801 cgacaggatc attgaactgg tcaaaagcag caacagaaca agcacctcca gcatgtagtg
    55861 caggaacaac agagacaatt tcgaaatttt ttgcttctgc cactgttcgt tctactacta
    55921 atgctcggtt caaatgttca caaccttgga cagctagcgc aatttcttta ggatccaaca
    55981 actcttttaa cgtacggatg atccattgac caacttccgc actggaattt tttccaatat
    56041 gtcctccaac gacttcgctt gtgctgcatc ctaaaacaaa aagatcacct tttttcaagt
    56101 gtgcctcagc caatatgtca ttaactattt cagtcaattg gtcttttaat tctttttctg
    56161 acaacttcat tttctaaaac cttctttttc aattaatatt gcttccgtgc ttgaatacgt
    56221 tgaagacttg cagttatagg aaaggcaagt cccattccaa tcagattttg gaagatgttt
    56281 cctggtatag aagccactcc agccggccaa tcatataaaa tccaaccggc gataccatag
    56341 ccgatgatca taactaagct tcctaataaa agccctaatc cccacatacg agtagaagat
    56401 ttgctgaaag aaccagcaac atatccttgt aacccgtgga tcactaatga aaaaactgcc
    56461 cactgcggat atcctgaaat caaatcgatc aatagaccac tggaagcacc aacaactaat
    56521 ccacccgttg gaccataaag aaaagcagcg gtatagatcc cggcctcgca taatgtcaca
    56581 aatccattcg tagccggtac agggatcaaa attgttaatg aaagggcaac tgtcaacgca
    56641 atcagcatag catagatcgt taattgctta gttttcattt ctcattcctc ctttttgaat
    56701 accgccagac cagacacttc cggtatgatc gttcatcagc acgccattag aaatacaatg
    56761 ggcaacaaat tttttacttt ctgctactgc tttggtaaaa tctgccccta acacaagatt
    56821 cgctgttatt gcagaagaaa aggcacaacc ggccccgttt actgttgtag tgtcgatttt
    56881 tttatgagaa aatacctcat aatgatcaat tgaatagaga agatccacag cttcatctcc
    56941 agccatcctt gtgccgcctt tgatcacaat gttttttgtt cctgaagcag tgagttcttt
    57001 agcgagatgg atcatttctg ttaacgaaga gggaagagct tgaccagaaa gtaaagcagc
    57061 ttctgccaaa ttaggcgtaa tcatcgtaac aagtggaaaa agttcttcga tgagctgcga
    57121 aatatacaat tgattgatca cagcttcagt ttctttgaat gccaaaacag ggtctaaaat
    57181 gattgggata ttttgttttg tttttatgaa gtctcgaaca accatgattg cttcacttga
    57241 attcaataac ccaattttga taccatctaa tgaagtcatt ttatcaatcg aaactaactg
    57301 tcgtttaagc aattccaaag atatagattc cagcataaat cgctcattaa tatctagtgc
    57361 ccctatagaa gtcaaggcgc ttagtccgaa tagaccaaaa ttctcaaaag tttttaaatc
    57421 tgtttggatt cctccaccag caaatggatc agaaccgcca attgtcaaga ttcttttcat
    57481 aacattttcc tccatgaata agagtataac gaaattttca aaaaagaaaa acagccaatt
    57541 ggcatatttt tatctcatcc aatttaaaaa aacaaaaaga aaagaaagac caaaatggat
    57601 ctttctttcc ttaaatagtt aggacttttg tgattttaat tctttctcga tttgaagccg
    57661 ttgcactttc attgtagctg ttcgcgggat tgcatcaaaa tcacggatga tagggacgtt
    57721 caagcgagga agttcatgga cttgctccca ccatcgattc caatccattt ctttatcagg
    57781 agctaaagca ataatcggtt gtggagaatg atttttatcc cttacaatca ctacttctgc
    57841 caaaaaatct aacgaatcta ataaaaagtc ttcaatagct aatgtactat tgatcgtttc
    57901 gatcaaatcc acttgccgat ctttcaaaaa taggtggcca tgcttatcca taaaaccgta
    57961 atctccgcta tcccaccaat cgccataaac attttcctgg aaccgtgcat cttccttgta
    58021 ataagtcaaa gcccgtcctt tagacagtaa ttgtatatga ccatctgtgt tttcaggtag
    58081 gacattccca actgaatctg taatccgagc gcttgtcatg tcttccagtc caacgcccat
    58141 atctcttgca tcacttgttt tcaaactctc tagtgtatga gccttaagaa tcattggtcc
    58201 gcattcactt tggccatata cttgaagaaa taccgcatcc tgtgccatcg acgttcgcaa
    58261 aaatgtcttc attgtctggt tgtttatagc atcaaaagtg gagtgatagt atcggatacc
    58321 cgaaaaagct tctggatgct ccttagcagt aaaacgccac tgaacaaagt tattcggatg
    58381 ggtctctagt gcaattggcc ggtgagcttc caacatatga acaacttttg aactttgtgc
    58441 gtttgctaaa ggcatcatag gaaatcccat ggacatcaaa gaagagatac caatattgaa
    58501 tctggaatgc actggagaga tgtgaaaagc gattaatttc ttttccgaaa tttttgtgaa
    58561 aactgttttt tgccatttcg tccgccatcc catagaatga gcagaatgac aaataagttt
    58621 agggatacca gttgtaccag aagtatgggt catataagaa atttcatttt tgtctaaaaa
    58681 tgattgagaa acagacaaag caggttgttc cattaaataa agaatag
//
LOCUS       DAYYKN010000003        55248 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000003 DAYYKN010000000
VERSION     DAYYKN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.003"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..284)
                     /locus_tag="ACHBB7_000120"
     CDS             complement(<1..284)
                     /locus_tag="ACHBB7_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208108.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSL"
     gene            complement(291..839)
                     /locus_tag="ACHBB7_000121"
     CDS             complement(291..839)
                     /locus_tag="ACHBB7_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM9208109.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(884..1216)
                     /locus_tag="ACHBB7_000122"
     CDS             complement(884..1216)
                     /locus_tag="ACHBB7_000122"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208110.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1230..1487)
                     /locus_tag="ACHBB7_000123"
     CDS             complement(1230..1487)
                     /locus_tag="ACHBB7_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208111.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1682..2224)
                     /locus_tag="ACHBB7_000124"
     CDS             complement(1682..2224)
                     /locus_tag="ACHBB7_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM9208112.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2245..3336)
                     /locus_tag="ACHBB7_000125"
     CDS             complement(2245..3336)
                     /locus_tag="ACHBB7_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9208113.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3344..5554)
                     /locus_tag="ACHBB7_000126"
     CDS             complement(3344..5554)
                     /locus_tag="ACHBB7_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9208114.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5570..8098)
                     /locus_tag="ACHBB7_000127"
     CDS             complement(5570..8098)
                     /locus_tag="ACHBB7_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9208115.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8150..8560)
                     /locus_tag="ACHBB7_000128"
     CDS             complement(8150..8560)
                     /locus_tag="ACHBB7_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM9208116.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8573..8791)
                     /locus_tag="ACHBB7_000129"
     CDS             complement(8573..8791)
                     /locus_tag="ACHBB7_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM9208117.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8788..9780)
                     /locus_tag="ACHBB7_000130"
     CDS             complement(8788..9780)
                     /locus_tag="ACHBB7_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9208118.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9859..10770)
                     /locus_tag="ACHBB7_000131"
     CDS             complement(9859..10770)
                     /locus_tag="ACHBB7_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9208119.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10789..11133)
                     /locus_tag="ACHBB7_000132"
     CDS             complement(10789..11133)
                     /locus_tag="ACHBB7_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208120.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11153..11431)
                     /locus_tag="ACHBB7_000133"
     CDS             complement(11153..11431)
                     /locus_tag="ACHBB7_000133"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208121.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(11790..12290)
                     /locus_tag="ACHBB7_000134"
     CDS             complement(11790..12290)
                     /locus_tag="ACHBB7_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208122.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12291..12836)
                     /locus_tag="ACHBB7_000135"
     CDS             complement(12291..12836)
                     /locus_tag="ACHBB7_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208123.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12833..14089)
                     /locus_tag="ACHBB7_000136"
     CDS             complement(12833..14089)
                     /locus_tag="ACHBB7_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9208124.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14450..14761)
                     /locus_tag="ACHBB7_000137"
     CDS             complement(14450..14761)
                     /locus_tag="ACHBB7_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208125.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14784..16259)
                     /locus_tag="ACHBB7_000138"
     CDS             complement(14784..16259)
                     /locus_tag="ACHBB7_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM9208126.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16708..17160)
                     /locus_tag="ACHBB7_000139"
     CDS             complement(16708..17160)
                     /locus_tag="ACHBB7_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208127.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17162..17527)
                     /locus_tag="ACHBB7_000140"
     CDS             complement(17162..17527)
                     /locus_tag="ACHBB7_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208128.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17626..21909)
                     /locus_tag="ACHBB7_000141"
     CDS             complement(17626..21909)
                     /locus_tag="ACHBB7_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM9208129.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(22056..22295)
                     /locus_tag="ACHBB7_000142"
     CDS             complement(22056..22295)
                     /locus_tag="ACHBB7_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208130.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22385..22627)
                     /locus_tag="ACHBB7_000143"
                     /pseudo
     CDS             complement(22385..22627)
                     /locus_tag="ACHBB7_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22740..22976)
                     /locus_tag="ACHBB7_000144"
     CDS             complement(22740..22976)
                     /locus_tag="ACHBB7_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208131.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(23987..24897)
                     /locus_tag="ACHBB7_000145"
                     /pseudo
     CDS             complement(23987..24897)
                     /locus_tag="ACHBB7_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(25093..26928)
                     /gene="lepA"
                     /locus_tag="ACHBB7_000146"
     CDS             complement(25093..26928)
                     /gene="lepA"
                     /locus_tag="ACHBB7_000146"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM9208132.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            27075..28295
                     /locus_tag="ACHBB7_000147"
     CDS             27075..28295
                     /locus_tag="ACHBB7_000147"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9208133.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28315..28809
                     /locus_tag="ACHBB7_000148"
     CDS             28315..28809
                     /locus_tag="ACHBB7_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM9208134.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            28787..29473
                     /locus_tag="ACHBB7_000149"
     CDS             28787..29473
                     /locus_tag="ACHBB7_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208135.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(29624..32233)
                     /gene="clpB"
                     /locus_tag="ACHBB7_000150"
     CDS             complement(29624..32233)
                     /gene="clpB"
                     /locus_tag="ACHBB7_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM9208136.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(32484..33530)
                     /locus_tag="ACHBB7_000151"
     CDS             complement(32484..33530)
                     /locus_tag="ACHBB7_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM9208137.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(33541..33813)
                     /locus_tag="ACHBB7_000152"
     CDS             complement(33541..33813)
                     /locus_tag="ACHBB7_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM9208138.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(33848..34756)
                     /locus_tag="ACHBB7_000153"
     CDS             complement(33848..34756)
                     /locus_tag="ACHBB7_000153"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9208139.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(34888..36120)
                     /gene="pepT"
                     /locus_tag="ACHBB7_000154"
     CDS             complement(34888..36120)
                     /gene="pepT"
                     /locus_tag="ACHBB7_000154"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM9208140.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(36135..37256)
                     /locus_tag="ACHBB7_000155"
     CDS             complement(36135..37256)
                     /locus_tag="ACHBB7_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM9208141.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(37253..37954)
                     /locus_tag="ACHBB7_000156"
     CDS             complement(37253..37954)
                     /locus_tag="ACHBB7_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM9208142.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            complement(38098..38538)
                     /locus_tag="ACHBB7_000157"
     CDS             complement(38098..38538)
                     /locus_tag="ACHBB7_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM9208143.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(38667..39593)
                     /gene="dnaI"
                     /locus_tag="ACHBB7_000158"
     CDS             complement(38667..39593)
                     /gene="dnaI"
                     /locus_tag="ACHBB7_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM9208144.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(39593..40966)
                     /locus_tag="ACHBB7_000159"
     CDS             complement(39593..40966)
                     /locus_tag="ACHBB7_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM9208145.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(40989..41480)
                     /gene="nrdR"
                     /locus_tag="ACHBB7_000160"
     CDS             complement(40989..41480)
                     /gene="nrdR"
                     /locus_tag="ACHBB7_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM9208146.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(41792..42124)
                     /locus_tag="ACHBB7_000161"
     CDS             complement(41792..42124)
                     /locus_tag="ACHBB7_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9208147.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(42176..42805)
                     /gene="coaE"
                     /locus_tag="ACHBB7_000162"
     CDS             complement(42176..42805)
                     /gene="coaE"
                     /locus_tag="ACHBB7_000162"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM9208148.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            complement(42769..43605)
                     /gene="mutM"
                     /locus_tag="ACHBB7_000163"
     CDS             complement(42769..43605)
                     /gene="mutM"
                     /locus_tag="ACHBB7_000163"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM9208149.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(43650..46295)
                     /gene="polA"
                     /locus_tag="ACHBB7_000164"
     CDS             complement(43650..46295)
                     /gene="polA"
                     /locus_tag="ACHBB7_000164"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM9208150.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(46421..47104)
                     /locus_tag="ACHBB7_000165"
     CDS             complement(46421..47104)
                     /locus_tag="ACHBB7_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM9208151.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(47097..47582)
                     /locus_tag="ACHBB7_000166"
     CDS             complement(47097..47582)
                     /locus_tag="ACHBB7_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM9208152.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(47889..49223)
                     /gene="murC"
                     /locus_tag="ACHBB7_000167"
     CDS             complement(47889..49223)
                     /gene="murC"
                     /locus_tag="ACHBB7_000167"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM9208153.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(49354..50115)
                     /locus_tag="ACHBB7_000168"
     CDS             complement(49354..50115)
                     /locus_tag="ACHBB7_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM9208154.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(50131..50613)
                     /locus_tag="ACHBB7_000169"
     CDS             complement(50131..50613)
                     /locus_tag="ACHBB7_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM9208155.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(50633..52813)
                     /locus_tag="ACHBB7_000170"
     CDS             complement(50633..52813)
                     /locus_tag="ACHBB7_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM9208156.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(52957..53688)
                     /locus_tag="ACHBB7_000171"
     CDS             complement(52957..53688)
                     /locus_tag="ACHBB7_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9208157.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(53757..54698)
                     /locus_tag="ACHBB7_000172"
     CDS             complement(53757..54698)
                     /locus_tag="ACHBB7_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM9208158.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(54878..55246)
                     /locus_tag="ACHBB7_000173"
     CDS             complement(54878..55246)
                     /locus_tag="ACHBB7_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM9208159.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT        15583 a        11889 c         8993 g        18783 t
ORIGIN      
        1 agtgaaaact tggtaatctc tttgtaatta attcttgtta aagaataatt aaacacttca
       61 tttttacttg ttattttttc attgactata actagttcat ctggtaaaaa aattaaccaa
      121 caaacatcga caaacagtaa atgatataat ccatttccac ctccaagcat attcaatact
      181 ggtgaacatg ccttagaaac acgaatatag ttatggttta aatagatatt ttctttaaac
      241 attgcttgtt taaaagccat atgctcttgt tctaaacttt tcataaactt ttactctaat
      301 gcacagctag cagcagctgc tgcaattcct gctacaatat ttaccttagc taactcttta
      361 gctgcagctt ttcctaattg tttttctgca atttttaata ataaatccgc agctttttca
      421 aaattaaaag cttttaatgc ttcaataaaa tctccctgcg tacctatagc ccaagccact
      481 cctcctccag gaagagaatt aatggctaca catccagcat atcgtttcca atcaactttc
      541 tccattctag gaacaataat atgttcataa aagactcttg ctgctcttcc ttgttcgtta
      601 tcttgactga ttcgttcacg aatagcacca aaatccttta tatggtatac accatgttcg
      661 tctaaaaaac caactgtatc aaaataaaaa gttaattcat ctactaattt attctttatt
      721 tctaaatcaa ctgtattcac tttttctgtg gcatacacac ttgttccagt actaacaaaa
      781 gtacttaaac ctaataagaa aacacctaaa acaccaaata ttttaattat tcttttcata
      841 atttctcctc cataaaaaat aagttacttt ttaaatatct tgtttatgaa ttcattcccc
      901 aaaggccttt taagattgat acagcagtcc gttcacttct tttagacttg tcatacaagt
      961 cttttaaatg atcccatatc agactagttt gtctatctag cttcaatcca ttaaacgcta
     1021 atgaacggtt cattttttct attactttag ataaagctac agaaattttt ataccttcac
     1081 aacgcgcatc ttggatttca ataatagtat catcaattag tttttgaatt tcttgcctat
     1141 caacatattc aatcttagaa agagaatctt ttaaagctac taatgattca acgatttctt
     1201 ttttctcttc agacattttc ttcctccttc tagtgacccg taaagccatg tagagcagag
     1261 tccattgaac cagtaattac actatctaca acgccattcc aatttactct acattttcca
     1321 tttttcagat ttgtcggtcc atacggatag cctttttgac attcaatgtt cggattatac
     1381 cagataggat tataccattc agccgcagaa gcagattcag tcgatacaaa caatcccaca
     1441 ccaacaaatc caatacacat cacactatag ccaattagtt ttttcatctc gtatccatct
     1501 cctaaaaaat agaatagttc ttatagttat tcataagaca tattttttcc gtttttgttt
     1561 tatcctttct gtcctataac aacttttttc aagtaaagaa tgacatagtt tttcttaaaa
     1621 aaacagtctg tttttttcta tttcttcggt gtataataaa tatgagtgtt cattttttat
     1681 tttaatacgc actccgtaca taataaatct tgatcgcatc aatcttttga gcttcttgat
     1741 ctactttcaa ttgaagtaat acaaattgag taaccttatt cattgtttct tctccaaata
     1801 acaaatattg atccggattc tctatatctt gtgtcacctg gtaaagtttc ccagttgttt
     1861 tcatttgtac atgcgggtct acatcaatac tattgtcttt aatacaacga tctgtcatat
     1921 agtccctcac agcttgattg cgtttataaa catctgtgaa attactccat tttctagcaa
     1981 aatcttttaa gaaatttttt gtcatttcat ctatttgcat ttgttgcgct gtctcgtctt
     2041 ctaatgaacc aatttgactg ttactttcag tactttgaag agttccactt gtttctttta
     2101 ctgcttcgat tgtttcttct gtcgttctca attgtgtttt ttgtttttca gaagaaaacc
     2161 aatgacaacc agtaagacct atcaaacata accaactaaa tactaaaatt atcccttttt
     2221 tcacgtttct agtcactgcc tttcttattt caccttacga accagactac gaatttttgt
     2281 ttgattgtac gtgcgttgat accgagccat aatccgtcct tgctcggcat tctgttccac
     2341 actagagaaa tgttgtccgc catatgaaac atctgttata attccggtgt gaccatagat
     2401 tccaggagac aatgcaccac ctgcttgcca attcaccaca tctcctgctt gtaaatcact
     2461 tggttttggg ttcatgatta cttgccaacc ataggttgtc cagttataat cttcccctat
     2521 taacatcgca aacatgtgtc cagaacccat taactgtggg ccacctaatt gagatacata
     2581 ccatgccgta ccaccataac attccccatt atttacggat cgtccaatga tccgatccaa
     2641 aaccttcgca ttgcctttac tttttcctgc aataagcgaa gttttttttg ttggtaggct
     2701 attccaatgc ttgatcgcaa aagtatcata ttccgttaag ttataggacg ttatgatact
     2761 cattgctcct cgataatacg gactcccttc ggtaatacct gctgccgcat agcctccacg
     2821 acctagaatc aataaacaac ttccaggatc tttattgccc acacattgat taaagttgct
     2881 ttggctcgcc accataaagc cataatagtc ataacaagct tttaagttcg gaaacttcat
     2941 atagaaacca ccttcagaac caccaattcc acgtgcagaa ccttgtgggt agggacaacc
     3001 aggaaaccag gtaatcccaa aaaagttcgt atcgtttctt ccagattgtg actgtccata
     3061 agctgattca tagcctaatt gagaaagaat cacactcggt aataactgat actttacagc
     3121 tgcaccaact gctgcttgcg catgttcatc tgtgattgga ccacgtcctt taatctctga
     3181 aaaaatcggt aatccttcgg tatattcccc attaaaagcg acagctagac ctgtagcttc
     3241 tcctccatgc tcagaaagtt ctcctgaaaa aaagaatggc aatagaaaaa agggaagaat
     3301 aaagaaaaga agacaaccta gacacccttt cttcatttct tcctcatcct ttcttcgata
     3361 atggtttttg gactgttcgt ccttttggta aagtaaaatc agttcctggt tttgcgtcca
     3421 aaatcggcag tgttttagga tccttttcta ttttttccga ttgctctgtt ggcttatcta
     3481 aagaagaaaa tgcaggttgt aacgattttg gtgcggaaat cgacggttta ctttcagacg
     3541 aacgttgccc cggttgttgc acagaacgat ttcctgatag agtggctacc gacgaattag
     3601 gaacagagta ttggttcaat aactgctgca ttgctggata actcccattt ttcgcctgat
     3661 ttttcacttg gcgaatctgt tgtgaaagag aaccagcagc tgttaattgt ggcacgggta
     3721 cttttccaga aggcaacgga atcgattgac caacaaaagc gataccttta tttaacaatg
     3781 gttttctttg attattccct tgttgcttac tcaattgtgc caatgattgt acctgttgtt
     3841 ttggtcttac tgaattcatg gacgaatttg gaatcaaacc atttgaaaga ccagaattcc
     3901 actctgtttt cgatttggta gaagacattt caggctctac cccatgataa cgaacatgac
     3961 gaccactata aggcaaggct tgatttgaac gcccacctag ttcaaaggga ttaaccactt
     4021 gcctcccttt taccgattct tgtggtgtac gtttttgttg aaacttttga aatccatgta
     4081 tcgctgcaga agcgagtgga ttcgttgctc gatacacatt ccctgctaat cctgcacctt
     4141 tgattgccaa attttgtcca gcagttgata gttgaccagg cagttgtttc atttgagaca
     4201 aagaatgact tgctatgttt ttcgtgttgt ttatagtttg atctgcagct cctaacccta
     4261 gtttctttaa taaatctttc cgttttacaa tgagtaaaat tgataggagt gctttgacaa
     4321 acatatttcc taaaaaacca aaaacagtat tcattggaat taaatcgtct actatgttat
     4381 agatcaaaaa tgccaccaaa ataatcacac tgtaaaaaga tttttgaaac atcaagccca
     4441 aaaaggactt taaggtattc atgagcatat aatcaaatac tggaacaaaa gaagcgagta
     4501 atgaaaaagg aatcacaagt aataaacaca acaccaacat ttgaaagata aaacgaatca
     4561 tgccaatcac tacaagtggt aatccaatac ttagattaag caatggcacc atagaaccaa
     4621 cgactgcttt ataaacagct ttagttggtt gaatatagac acgataagcc ttctttttcc
     4681 ctctatcttc tcctgcagca gtggtcactt ttgtctcaat atcagataat tcttcacttg
     4741 acgcttcctt ggctagaaat tcttgtggtt caatctctgc ttgatacaat tcttctaaat
     4801 ttgttgtccc ataatttaac aatatatacg gatcaacaac agccgcttta taataaagtt
     4861 ggcgcacttt ttgaatggct tccttttgtg tcgttggcac taaaacacca ttgacatctt
     4921 ttgtcgccaa ttcatctaga ctatttgtag cagaaaaaat aaagccttct acctccacgg
     4981 acaaatcatt taaccctttg gtaaaagctt ctcctttact aggtacgtta ctaccatagc
     5041 caaaccaaac cattgaaaaa atgacaacga agagaaatcg aataaacacc atttttgcgt
     5101 aatgtttccc tctaaatata cgaagagtaa atagatataa cacatacaag acgacaatcc
     5161 ccgcaccaat aggatcgtat atttttgaat aaatagtatc cgaaaaagtg aaaaactggt
     5221 gaattaactc gttcattgca gctccttcat agagcttctc aatgacaaaa tcaaggcctt
     5281 gccaaatcaa tttacttaaa ctaaagaaca tattcgtcat catttttaga aaatctgtgc
     5341 catttgcccc aaataacatt ttgcctaatg aaaattcact atcagataaa taagaagaaa
     5401 aggtatcaat atgaaatttc ccaatctcat agatcgattg atccacctgc ttttctgccg
     5461 catacaccaa tccaccaaca aaaagcagtc cgaatccaat cgttaaaaaa aacaaaacgc
     5521 accatttctt ttccttatgc tttatataag ccattgtttc acctcatttc tattgcatga
     5581 ttaacgtatc ctgttcttct acttgagagt gacttgtttt ttccgttgtt tgtaatgcag
     5641 cggtcatttc ttcaaacaaa ctgtgtacat tgagttttcc tacacgtcca tagggatcta
     5701 acatccaaca ctgtccttta atcatgtggc tcatttcagc taagttcgct tcatttactt
     5761 ccaaacgtaa atgattcaaa atcttttctc gctcactgtc ttcatcaaaa gcaaagacga
     5821 caccaaaatt ccctttttct ttatctgaac ctaagtcacc aataaattgt gtatctaaca
     5881 ctaattgatt catctgggaa cgccccacac gtgccattgc tgcaataata ctacgtcctg
     5941 cgttcgaatt attaaatacc catgcttctg taaaataaat cgctgttttc ttactactat
     6001 ctcgttttcc aaacatttca caaaaacgac ctaaactaat catcaaacac aaagactttc
     6061 gatccgcatc tgtatatagt tctggtcgga cattgtcatt tggtaatttc aaatcttgga
     6121 tctctaaaat cgttgttttg gcattaaagt ctaatcccgg gttttctcca taagaaaatc
     6181 ctaaacgtaa aatcgaatct tctaccgtca agcgtaaaaa ctcaccatat tctcgaacct
     6241 ctttttgttc catttgttct aaatagtcga ccacatgcat caagccaact tgttgtccgg
     6301 cttctttttg aactaaaact tcttggattc cctttaatac cgcccctttt aaaaggtgat
     6361 tggtacctaa cggacttaac tcatcaatca tatcctgggc tacttgtttc gcatctactc
     6421 gggataaaca aacaattgga tctaatacac cataattacg gggatttgta gcatctaacg
     6481 tgacataact cattttttgc aaatgttcta caaacaaagg gtaatgtttc tgataatacg
     6541 ggtcttgaat gagtttcgta aaccatttgc gcttttctcc tttgggatcc acaaataaac
     6601 tttgtacatt cataaacgaa ctatacaaaa agagcaagcc aactaaaaag gtcttccctt
     6661 taccggtttt tccagttact gctacatgag gcgcatcaaa caacgcacct ttgattcctt
     6721 ccgcaatggc tagaagattc aaaaagacaa aacgatttga cgcataaatt aattcttcta
     6781 atggtgtttt ttctcctcga gaaccaccat cccctaatgt atcttgccga ccaatatacc
     6841 agccggattc catacctaat tctgatactg ctgaaaacaa ccattcgcca actgcttcta
     6901 cagttgttcg gtgcttccaa tgtaactgcc gtcccaatgg ttgccctaat aaacatttat
     6961 agaacaattc tcgttgatca ttttttgcac ggtggatatg gataatcgta tgaagttgtt
     7021 gaataaccgt tgcactattt tgacgaactt cttcaaccgt ttgaccgtat acaataaaac
     7081 atgcgatcca atcgacgaca ggttgcccat tatctactcg ttgctttaaa tgttttacta
     7141 atcctcgaac agttaataag cgttcactag gttcttcatc attttgaacc aattccttat
     7201 gctctgtttt gaaccgttcc gatagccaat tcagttttaa accaatacct ccctgtcctt
     7261 tcaagcgtgg atagtgaatt ttcattcgca attctgctgg gaaagaaaga tcctgagcaa
     7321 attgaaatag atcacaattt ctcatatttt caggtgtttc cccaataggc aacaatgcta
     7381 aataactact ccctaactca tcttctaatt gaagaaggcc tttatgttcc ttcggtatac
     7441 gtacatacgt tccttgattg tctttcagta cttcccaatt attttgttct tctactttgt
     7501 gagccattcc tcgaatatat tgcagacgat tgagatacaa cagctctttt tttactaagg
     7561 gacgaataga aaagctataa agtaattgtg tcacttcttt tacataagga tcactttcag
     7621 aaaacaaagt ttccaaatca tctacttcta atccaaataa gcgtgctaaa cttcctgcta
     7681 aatctaacca gattagattt attacatcat gaaaactctc caccgttgct ttttctttga
     7741 ctttgattcc taaaataaat gaaggacgat agacttgacc ttctcgttcc aacaatgtca
     7801 ctgtttcttt tgcataatac gcaccaattg cttgactatc gggagaaaat ccctttccta
     7861 atgtgtcaaa cttcgtccgt aaattcattg ttcgtggata catcaccaac tccacatctt
     7921 cataatctgc taaaagcaac agtaaacgag ccatcatttc ttttatttcc tgtgtctttt
     7981 ttggtgtgtt ttttgaaatt gttcgtggaa aaacatgata gtaggcccag atacaatcct
     8041 ctccatttaa caaacaattt ttcttgaatg cttttactgg ttcttttaca agcaccaatg
     8101 aatttcacct cccaataaaa aaggaacaac cagttgattg tccctttttc taatcctcta
     8161 aataaacata cttattcaca aaaagtaatt cttccaatgc attgtttgat accttgtccc
     8221 agttataata cacacaatca ccattcatca tacgaatcat ataacaaaac caatcccata
     8281 aataaaaatg cacttttttt ccatcaggtt ttagttttgc cagtgctaac acacataaat
     8341 aggggagtcc tatataaaaa acaactcctg ctggtggaaa aaaagaaatg acccaaccaa
     8401 taaatgaacg taaaaataaa tattcaaaga ttacgaccaa caaaaaagtt gctgcattga
     8461 ctaatgcaac tggaaatgga agacgccaat tgcttgtaat ctgttgtacg cgataaggtt
     8521 ccgcataaac aatcgaataa tcataaggtt cccaatccac ttcactatcc tcctaatcca
     8581 taaataattt ttcaatgatt ttaccaaacg catcaaatac acgtgttgga tcatttaaga
     8641 agaaataaac tgcggcacct gctgcgatag atattaatgc atttcctact tttgatttca
     8701 taaaatttcg caatcccaga taaacaacca ccattattaa aaaatagcca ccttgcgtta
     8761 atataaaatc tacaattccc ttcagtgtca tttccgttct tctcctttcc tatccaacca
     8821 attatgtgtt aatttttcaa cataatactg atttgttttt ttcgtaatga caatcgtcat
     8881 ttgttctttt tgtaaaagtg acgtatcacc atctcgaaag acgaccgttg cttttacaat
     8941 aaaaggtttt ttcgtttctt tataacgcaa tactttcatc tctacttgat caactttagc
     9001 tgtttgattg agtccttcag gttctttcat catgtatgac atatcttttg cattgttttt
     9061 cgcatactta tctaagaaat cttccacaaa tccttcaaca tctttcgctt ccgacgcatc
     9121 aaccgtttcg tataatgtgt catcataagt aatcattcgt tgtttttctg tattctttgg
     9181 tagtgccgta aaataaggat aagcaataat acgacagccc ttgtttgaaa tagaaaaggg
     9241 aagattgagt acttgtgtga ttgtcacact tgtttctttc ttactctctg gattcatcga
     9301 gcgaatatca taagttactt tataactagc agttggaatg gtttcaattt cttttaatcc
     9361 aataaacgaa gcagcagtta attttcgaac acctgaaaca cttcctggtt cttgaataaa
     9421 gttttgtgca taaaactctt tttttaatgt ttcttgacgt tgttgttctt gttcatgatt
     9481 atcgaatgta ttcatataca aagcaacaaa acgattcata aaggattcaa tttcaggtgt
     9541 gtacgttaat tctgaccgac tttgtacttc tttcttttgt aattgttgga tttcatcttt
     9601 caaaaacgac acttgaacct tcgttttcat ggattgaaaa aatgccaagc cccctgaaaa
     9661 acaaagaaat aaaaccaaac accaaatcca tttcactgct ttttttgaat ggattttctt
     9721 ttgcttcggt actttctctg tataaacttt ttctttccgc cgtagttgtc gttttttcat
     9781 aagctactcc ttctagtatg tttttactag tacgtacgat tgacaacgat aagcttgttt
     9841 ttctgaaaaa tagtccattc aattaccctc attttctatt ccttttctct ttcttttata
     9901 tccaacaaat ccagcaatac ttgtcaacac tcctcctaga acagaaagtg caacatgtat
     9961 ttcttcacca gtctttggta agacttttgt ttttccatct gctgttttga tttcaatatt
    10021 gccacttgga agtttcttta caggaatcga caaatccttt accaaattcc cttcactatc
    10081 ttggattaat ggaataccat cttttacacc aacaattgtt tgatcacctg ctacttttaa
    10141 tggggtggtt ggtgttggaa tatgggtcaa ttcctctaca ttactagtga caatcggtac
    10201 actcattcct tgagaagtat tcgtcgtaat ttcaccgctt ggggttaccg atactgtgac
    10261 ttcttttgct ggaacaatag gtttattttc ttttggaatt gcttctgatt tttcaacagg
    10321 ttgtttcggt tcttcttttt gttcttcact ggtagacgac atcgttggct gttccgtact
    10381 tgacgaatca gtattctttt cagaagaact ggttgttcct gattgttccg gagttgtgac
    10441 tatacttgaa tccgttgttg ttgaactatg cacaccttct tcttcgatcg aatcagtcgg
    10501 taaagttgat tcaggaaact ctggttctgg tgtcactggc tctttttcag gtttctctga
    10561 ctgcaattgt tcaggttctg ctggtttttc cgttgttcct ccatttagct ctgtctcaac
    10621 atctggtaat ccttcccctg gcaatgtttc tacaatttct gacttaggtg tttctacagt
    10681 ttcatctgca taaaccaatg aactccctaa ttgaaacgaa aataatgaaa ttcctagtgt
    10741 tgttacgact gctaatgttg tttttttcat gattattcct ccaatttttt atgcaattcg
    10801 tgttttgttc atagacgccg cactaatatt atctgaacga taaaagaact cccgtccgtt
    10861 ttcttctaac ccaacatatg gattattttt gaataattga atcaatagtt cactggtgtt
    10921 gtattttgga tagtttgcaa aaaaataata ctgtgcttgt cgcccatccg aaaattcaac
    10981 aagtaattca taacgatacc gcttcaatcc ttttcgttgt tttaaaggat ctctatacca
    11041 acttgtccat cgttccattc gtggatagag atagacacga tacacataga acaatgccat
    11101 accacatacc accaaaaata ataatcgcca cataaaaatt cccccttttc ttctaccttt
    11161 cttgaatcat cgaacattca taccctctat agagatttga aaaatccatc atcaattgtt
    11221 catataaacg ttgttgcaaa tagcgattgt ttcgaataca taattcttca tagccatcaa
    11281 tcacttgatc ttctttttca tagcatcttg tagctctatg acgctcagct tgcaagacat
    11341 aaccaaactc tgcaccatat cgttctactg ttaataactg ttgactacgc aataatgcaa
    11401 tataacaatc acaaaaatgt tcaaccgaca tttgattttt ctcacgtttg tgaaataggt
    11461 attctttaga aagcacatta ttcgttaaac aatctgataa atagcgataa tctggcggtc
    11521 ggttattttc atctgtgaat ttttcccata ataaactgat ttttattcgt tcagcggcta
    11581 gatagtcttg ttcgcgtgga taaaattcat caatcgcaca aagtttttta tcacctgaat
    11641 aaacaaatcc atataatgcg aaacgaactc ttccttttca attggctctc ctaaaaattc
    11701 cagtgacata ccatcttctc cttcctaaaa aaaataaaaa agcagaaacc gatttggctt
    11761 ctacttttct ttccttttat agctcttttt tacggaagaa tccgcaaatg acattttttc
    11821 aaaaaatcaa tcggcacaag ttcacgaaaa cttggagcaa ttttggatag aatcacttcc
    11881 ccatcttgta aaatacaatc ataaaaatca tcagttactt gatacttacg cttcttacaa
    11941 taattgataa aatagtagcg atcattttct atttccattc gttgttctac taagcgcata
    12001 cgaacatcat catagaaata agccacaaat cgcccttcaa gttcatgttt ttcaatggga
    12061 tactcttctg gtaacaatct attttttcgt cgtttataca ttgctggata ccttagaaga
    12121 agttcatcta aacgtctgtc agtatatggt aactgacgaa tcaacgattc taatcgttct
    12181 ttatacagat agaataattg gcgcaagcaa tagtgtaccg cctctggatt ttctaatact
    12241 gttggatcac gtgaccaata cgcaccaaca taaatcataa tttttttcat ttagacaatc
    12301 acaccagcaa tactaatgac cgatccacaa taggtagaac aagtggcata gcgaccaatt
    12361 tcctctaatt ctatctcaat ctcttctaaa gaaagatatg tctgttcttc tgttggctgg
    12421 cgactcattt cccaagtgac agtcaccaat gaaatttctt ccataagagg tttttcgctg
    12481 ctaagtgctg ccacacagtt ttcatatatc ttcattttat cgcagccttt tttaaccgct
    12541 aaaatcgcag gtattggcaa tgaaaatcga taaaagcgaa tgaattctag ccttcctttc
    12601 cttcggctaa attccaactc ttcgagttct tgttcgtatc ccttacgaac ttctaataag
    12661 cgtgcaattt cttgccagtt ctctttttcc tgataacgtc cagcttttac cgcttgttca
    12721 atataaggaa ctcgaaaacg aaaataagac aaatcaacca tcttttcatt taatgcttcg
    12781 attgcagcct ttaaatccaa caggcgcagc cattctgttg tcgtatatgc attcatgcaa
    12841 ttttccctcc tgacgttccc atccattcta gatagttttg ttgctcgtca tttagttcgg
    12901 cattatcaat cccttcttgt acaaagtctc ttcctaaaat acgatctgct tcttgtaaca
    12961 actttaatgt cggagctact tgtttcaaaa accagcgttc tgaacgatct aatgagaatt
    13021 tttctggctt tactgtaaaa tccaattctt ccatcgaacg aaataatttt gcccatcgtt
    13081 tatcaacaat ccgtgcatca ctttccagtc tatcccaaac aataagtttt gcattaatta
    13141 aaccaacgcc aatctctcct aaacgtttcg gtgaatcacc taaaataaat gcttctactg
    13201 cttgtgttgc tttctcattg gctaaccgta actcatatcg attataaata ccatatttcg
    13261 ttaatgcttc taattctgaa attttttctc ggttcgcaat ttcaaaacgt ttctcataaa
    13321 aattaaaaaa gagaggactc tttcgcgatc caaaatacaa agataatcct tcacttcgaa
    13381 aatagccatc atctaaaaaa caaccgccaa tattctggaa tctcttaaag tccatttcta
    13441 ataaacctgc tttttgtttt tctagtaatt caaagagatc gaaatgttct tccgtttcca
    13501 atccccaaag ttcatctaac gcaatatcga atcggctaca ttgcacacgt gtataaccag
    13561 cttccaactt tccttgattt ttccaaatgg aatatagcca tgttcgccaa tctgtttgtt
    13621 gttcacgcat gacttcctca tattcccggc aaccttgtcc tgaaaattgc acaagtacat
    13681 tttctgttac agttcgtttt gaatgccaga acacccagat attcccacga acagaaagac
    13741 agtcaaaaag atacaagccc gttggacgtg tttcaaattc tgtcagatcc attcgtaata
    13801 cacgttgaat cactgttttg tagtctgtcc caaaaaagga aagacgcaaa taatcaatca
    13861 ttgctcgaag atctattccg cttttcatat acaatcgaaa gcgttccttg attcgttgtt
    13921 caaaatcctc tgggattttt cttttccctt gttccatctt tctaatgtaa tcttttgagc
    13981 agcccatcaa ttgcgcaaat cttgcttgtg atagcggctt tttatctcca cttatattat
    14041 gaattgcact taacttcttt ctgtagcttt ttagctcaat cggtgtcatt ttctcgctcc
    14101 ttttataaaa taacaaaatc gatttttttt aaccctttag aaacaccgat atatcaacta
    14161 gtccccctcg ttttttggga aaagtagtac aactgctgac cttagataat cacttgatac
    14221 aaaagggttt ataaaacttt tttctatgtg ttatgttatt tctgcccccc atattatata
    14281 tcggggggct ttacacaaag aggccgtgcg caagcgcacg acctctttgt gagctgcaca
    14341 cgctgctagc gagtgcgcat tataggccca tgcttcgcag ccttaatgcg caccgccagg
    14401 cagagtgcgg ctaccagact acgtctagag ccctcccgat agataataac tacaaaatta
    14461 aatgatctaa ttttgttatt tcactatatt tttcaggaga aaaaacgcga taaattgaca
    14521 aagttaaatc ctcttctaaa tctactcgat aatgatacaa tcgagtcaat cgttctatat
    14581 cttttttcgt tagcaatact tgattaataa cttttcctgt aaaaacacgt tctttgcgca
    14641 aaaagcggta atcagttact tgttcaatac gattcaacca atcataaaac gttctggaag
    14701 aaagagaagg attaatcatt cgtattgctg tagctactct ataataacaa ctctctgcca
    14761 tttaacttca cctccttagt tgattacaaa atatgcgcat cagaaaaact tgttcctttt
    14821 ggatcttttg ctaaatacat ttggcgctct agttgttctt gtaacaactg attatcatct
    14881 cccatcgttt gatcttgact atcctctttt actgtaaaat catagacaat catttcagta
    14941 aatttctcaa attctttgat aaagtgataa tctttgtaaa caaatggcgc aaaaaactct
    15001 acaggtacat caaatcctgg aaaagcatat ccacgaccag gttgatcttt gttgtaaaat
    15061 ggcttatttt taaaatcttc tccaaaagtc attaaataac caatgggaga caatcgacca
    15121 acagaaatac ggaatgttaa attatcccga acaccacttg gaaaatattc tgcatctggt
    15181 cgttgcattg ctaaaataat gtacatccct gcttgacgac cctttaatac taatggctga
    15241 atcaatttat cgactctatt tctcgcttcc atatccaacg aaccataaaa agctgcccat
    15301 tcatcaaaaa agataaaatg cggtggtaac ccataataag cataattttt ccctggttca
    15361 tatttaggat ctttcatggt tttcatatag gcataccgtt gattcatttc ttttaatgca
    15421 tttcgtaaac aaataatcat ttcttcattt attttgtaaa acacatgtcc tttgaataca
    15481 tcgacgtcac ctaatgctgc taaatctgcc gcttttccat cgcaaatatc tactgtccca
    15541 acacttaaac aagcttgaat taaagaatac aataaatagg ttttcccacc accgattgca
    15601 ccagcaatca acatatttgg tgattgatca tatacccaat ccaaagtttt cattaaatga
    15661 atacgtccat tttctgctat acattgatca ataggaatcc gaccagaatc tgaaaggaat
    15721 acataattga ttcgggcttt ttctcgttct tcacgaatta aatcacgcaa aaatacttca
    15781 gataacaaat gacccatttt taaaaatcgt tcgtgaaaac gcccaccatc taaaggaaaa
    15841 gaaaccacta attcttgttt cttttgataa aaataaactt ttggtaatct cattttttcc
    15901 cttgtttgtt tctcactaac ttgtttcttt gtataaacaa accgattgtc aattaacatt
    15961 cgaacaagta actgacgcca ataaacagag gcaaaatatc cttcttttag tcgcgaccaa
    16021 tgcatcaacc ctaaaattaa cccactaacc agcaacaaat acaacacaat acttattcca
    16081 ggaataagat acatttgcca attcgaaaac gtacttaaac gtggataaaa tactttccaa
    16141 ctaaaccaac ctaacagaat caaaaatgga aacaaaaaga aaccataata gcaaaaccat
    16201 aaataacgat tgaaatagcg aattcgccga ccacgatact tgaaaaaatg agctttcaat
    16261 tgtcacattc ctcctttctg tttttacttt tcctgtgcta aaaacttttg acaaaaagca
    16321 actaatacag gaatatcaca tttagtaaaa aaacataatc gaaaaagatt taatcgattt
    16381 ttataagggt gtgcacacat attacatatt cgtactatac gaatccaaat cacttcctga
    16441 tgatcttgtt tccactggta cggtaatgaa aaacgaacaa gatcacgtaa aaacatacac
    16501 tgcccaataa ccgcacaaat atatgcattt gtttgtacaa aaatcgttgg attgtctcca
    16561 aaatactcaa tcaattgttg gatattttga taacaagcac aatctctcaa agtatttgat
    16621 tggttttatc tgaattcaaa atctcccctt ccttctatct tagataaaaa gaataggaag
    16681 actccttcct attctttcaa aaaaacctta ttttttctct ttttccttag aatcaacact
    16741 tggttgtttt tgatctaaac ctttttcttg tgattgtttc atattaggat tatttgttgg
    16801 agcttctact ttctctaatc ctttcgcata aatcactaaa tctgaatatt cacgattgcc
    16861 atcttgtgct ggaatatata ccacatgata ttctggatca attaatttca caggatcacg
    16921 tcgtttaaaa ccagtcaaat ctacatctgc tggaaattta actcgtcgtg caatctttgg
    16981 ttcatctgga tttccaaaaa ctaattgtgc tcgctgtgat cgaatttctc ccgtataatc
    17041 gcctgtttca ttatcaaata cacgatcaga ctcttctaat gcgccaaagc gtacggctcc
    17101 taaacaagtt tcatctaatg cttctgtaat gtcataatta tttggaaatt ttaatcccat
    17161 cttacaaaac ctccccaatt tttacaccag ttaaaaagac accaacatct tgatagccag
    17221 ttgttcgacg ctctgtttgt cccatatcat ttgtttcacg actattcact cgttctttac
    17281 gaaaaacaaa ataagcttct tcaaatgaaa tacgcacctc tttttctggt aatcgtaatc
    17341 ttttatcctc ttcagctgtg agataaacaa aaaaagcagg ctgttcttct cgcgaacact
    17401 ctgcttgtaa ttgaacatat cgcccagaca caccattaat ttcctttggt ggattaggtt
    17461 cccgtgaacc caataaaaat acttgaccta aaaactcttt cttcatacgc aatgcttttt
    17521 ctgccataaa gtttttcctc ctcttaaaaa ccaaaataaa ttctaaatat ttctacatta
    17581 caatcaaaca agcggttaaa aatcctaacc gcttgttcct catgactaat ctttggcttt
    17641 acgtacttta taaagtgtta tacctaaagc aatgactaat actactaatc caagaaccat
    17701 aagtatacta cttgtttgtt cacctgtttt tggcaacatt ggtacaacag tagctggttt
    17761 aacccgttgt tctggtttca ctggttgcgg tggttttggc gcttccacaa tttgaaccgt
    17821 ttgatctttg tttgttagat ctacattgtg tttcgccact tcttcaaacg tttctttctc
    17881 atcttccgtt ttcgcttgga gccattccgc aaaactcaag ccttttcctg caagattgcg
    17941 accgtccact aagaattcaa ataagaattc catcatttct ttatcggccg caaagacttt
    18001 ttccgcatga gtgccttcaa atggtgtgcc gtctggtgtt aagtagcctt gaacaattaa
    18061 gcgatagatt tgaccaatcg caagaccgtc tactttgacc cattcaagca cagtttgatt
    18121 tggcaaggcc aagatctctt ttccgccatt tttattcgtc gctttcgtcg taatggttgg
    18181 attactgatt ttgatggttt ggtcattgtc ggtaatatct gcgtgagaag ccaataatac
    18241 gccttctcta aacagatctt caaagaccac gacttctttg cctcgcaacg cacgtgcgtc
    18301 tagaataaag tctagattga ctgtaccatc cgttgtttct gccacaaacg ttaaggtact
    18361 tgtgacttct tttccatcta ctagcactgg tttttccgtt actttgtcca ttagaacacc
    18421 gttgactgtg tatgtttttc caacgaccaa atcgtggtat ttcacagcgt ctgtaatggt
    18481 gactttcgtt aatggattaa cctctttcaa gccagtgggt ttatgtgtag ccgttgtacg
    18541 tacacttggg ttagttactc gtaccgtttg ccctttgtcg ttgatgtctt tatggtttgc
    18601 tagttcttca tcatttctaa aaagactttc aaacacgacg atttcacgac catttatacc
    18661 tgtcaaatca aaggtaaatg gtacgatcac cgtttcattt tctgctttgg caataaagcc
    18721 aacactcgtt tctaccgtat ttccatgcac aagcaagggt tcaccggttg ctttatccat
    18781 taagatccct ttgaccaagt atgcttttcc tggaagaatc tcggtaattt caacggtatc
    18841 gtttaaggta aacaatcctg ttgggtctgc gactgtcaat ccgtcttcaa agacagccgt
    18901 ggtatgaacg gttggattat tcactttcac actttgggct tcatcgttga tatccgcatg
    18961 tgctgcaact tcaatgccat cacggaaaag tttttcaaaa acaacgatcg tttgaccttt
    19021 cagaacacta gcattaacca caaattcaag gtcttttgtg ccattgactt tgtctgcaat
    19081 aaaggccaag gtacttgtga tttctttgcc atcttgtaaa attggcgcat tcgttgcctt
    19141 gttcatcaat actcctgaaa tggtgtattc ttttccgaca aacaagttgg tataagaaac
    19201 actgtcttta atgacgatcg tttctaatgg atcaaccact ttttcaccat cggctttatt
    19261 tgacgcttgt gtttctagtt ttggtggaat caagtaatta cgtacagaaa ccactaacgt
    19321 ttccccatca tctggcgcat tttgtttgaa tgcaaccgtg aatgcttgtt tatctgtatt
    19381 tggttggaaa ctttctaatg gggcattttc caagaagtaa tagtcatcgg caactagacc
    19441 tttcactaaa atttccccgt tttgatcagt ggtatattca ccaattgctt gatttttacc
    19501 gttatacaag gtaaagttca cacctgcgag cggcgttttc ttcttgttcc attcttcata
    19561 cttgaataat tttacacttt tccgtgcttg acggttcgca aaatggatca ctttgatcgg
    19621 ttctgtatca ttggtaatcg agaacactaa tgggttttga ttcaaaacgt aatttacggg
    19681 ggctttgatt tcttccaaac gatagcgatc ttttccataa gtaagtgtac cgttagttag
    19741 gaaaaagcct ttttcattgg ttttaaagat atccgttgtt tctgtatcat ttggaatctc
    19801 cattgtgaca taacgattgg ctaaagtatc aaaaatcttg aattctgtat tagcaagtgg
    19861 aatcactttt cctgtttctt gatccacttt caccacttta agacgttgtt caattacttt
    19921 atcttctaaa acataatgga aagtttgccc attttcagaa attgtgactt caagtggttc
    19981 aaatagttta tacccttctt ttcctttggt ttcagaaaga atgtacgtat caaagacaac
    20041 ggtattttta ccaaaagtag cataaccatc attgtctgtc acaacggttt tcaccacttt
    20101 gccagttgtt tttgacgtca aagtgatttc aatgccagct aattttggtt tgttatctgg
    20161 attcgttgaa tcgactaaat ctttattagc aactttcact aaatcaatgg ttcctttgat
    20221 cacttgatct tttgcggtcg cattgacagt ttttccaggt tcaacaacaa tttcaattgc
    20281 ttcatcagaa agcacataac cttttggcgc tttgatttct tttaaaacat atttgccagg
    20341 atcaagatct ttaatttctg cctgtacttt gccgttaatt tttttcaagg taatttcttt
    20401 aatctttttc ccatctgctt gatacaaacc atagaccgca ttttctaaag aagctgcacc
    20461 ttgtgctttc acaccagttt ccgcatcttc tttaaacaca cgaatgtttc cttgttttag
    20521 aatttgtaaa ttaataacag acgcattagg ttctgttgga tgtaacacac caattgtttg
    20581 agctcctgga cgacgatagg ctaatggcgc accttgaact ttaattttat tcaattgaat
    20641 ttttgcttta tccggtgcct cttttgtgac tgtaatccat acttcattgc cgtgaataga
    20701 tacattgaca ccttttggtt tttgaatgtc tttcacatat ttcaaaaaat catttttatt
    20761 ctttactttc aacgtttcgc caatttttac cgtatgtgtt tgattattaa aagttgcttt
    20821 ttcaccaaag ttattaatca aacgttgaat ttctgctttt cgacgatcat aatcaacacg
    20881 gatatttttg attgggattt ttgtgcccaa agcttcccaa atcataaatt gtgccgcaat
    20941 gtattgttca tcggatttgt ctttatttgt gacatatcct aaataagaga tcaagcttgt
    21001 ttttttccgt acatttgaat ttttcaaata gtttttcaaa gcagtagctg tataaacttg
    21061 accttctgcg gctgagacac caggctcaat acaaaacgcc acttgtccat taatctcaaa
    21121 acgaaccata taatttaaag tgaagtttag attgttcgga ctagaagtaa caccaatatt
    21181 ttcgccccaa tcaattttcc gaccacctaa aacagctgca ttggttgcac ggcgaacacg
    21241 aaccgaacga ttggttggta ctgttactaa cagtgtaggt gtacctgttt ttggaatgtt
    21301 ctgctctttt ttcttttgat tttcaatact agccgtagat tctttgtttg gtttcgttgg
    21361 ttgattttga ctagctgcgg ttgaagagat tgttttttca acgttttcct cattttttcc
    21421 ttctttgtca gcagtagccg ctggttgttc cactgtttct tctattcgtt tcttttcttc
    21481 cgcttgtttc tcagaagact ctggctgcac agaaggcgtt gcttcggcag ctttttctcc
    21541 ttctagagtg gacgtttctg aagcaggggt tgtaattgag gcagtttctt caactggttg
    21601 tgaagtacca ggactattcc ctgggtgttg tgtttcccca atcgtctgat cgagagtcgt
    21661 tttggaattg accgttggcg cttctgtcac aggggcctgt tcaacagtgg cttcaccagc
    21721 tgggaccccg gcttctgctg gttggacttc tgtcacttca gaagcgactt gggcaatggc
    21781 ttccatcgta ggttgtatag ccgtcgtttc ttctgcatgt gcttgagcag ttcccaccaa
    21841 gaaacagaat tgagaaaaag ccaccaatgc gagtgctttc aaccaattca ttttcgtttt
    21901 tgcatacaca agaattcctc cttttcctga gaattttata taataaaaca aaacgccgtt
    21961 accaaaagtg ataacgacgt gaactgtctc ttctattttt atctggcgct cttatctgaa
    22021 cacatttcat caatgatttt accctcccac tataattaca gaacaaaaaa agaacctgct
    22081 tcgtggaaat caactgtcca tttcccaatt ggaataccag cttttttggc aacttcacca
    22141 tcaccaaatc tcatgccata ccaattacta ccttcatcct taatttcttt ccaaccatat
    22201 gacatagctt catctaatga atcaaaaatc atgacactat tgccaattgt tacaggatct
    22261 ggcttaagat cttctggaag agaaatatca atcataccat aatgatccga ttcctcagta
    22321 ttattgtcat tactagctac tggtttggtt gttctactgg cttattttga tacaacaagt
    22381 actcttattc ttcttttact tctccgtttt caatttccca ttcaaaccac tccgcaaagt
    22441 cttgcttaaa accatctact cgatacctat tttgtaaata ggcatcttct gacgtcgtag
    22501 gatctaaata ggtaggttta ttctctggac gagcatagac acctacccat gtttcgtaat
    22561 ctccttcaaa ataactatct accaaatagc ctttttttct aagaatctcc cgaacttgat
    22621 ttttaattat tgatacttgt tcaaaaccat cactcattct atcacctcaa ataactctag
    22681 attctcataa atattcccga caatttcaat cctatcaatg atctctaaca aatcgctact
    22741 tatgcttccc cattcgtata agaatttccc ttccgcaaaa taaattttcc ataaacttct
    22801 tcatgatcgt cccaaccaat atcactctcg aaaaattcca cgttgttctt atcttttagc
    22861 tcagtaaatg ccataagttc aacatgtttt ccaacctcga aacaatcaca ccacccatca
    22921 accgttacac gtgtgccttc aatacctaag aaactttgtc ctcgtgtatt aaacactaaa
    22981 actttccgca ttttttgctc attaattaat cacgcgcgaa atctaggaat cacaacttat
    23041 tcgcttcttc tttataaata aaaaaactcc aataatcgga aattactatc ttttcctcat
    23101 tcctttttta aatcttcata cttcttctta tctttgtcct aacctaataa cacaaatttt
    23161 gaagaaaaac tcagtaaaac ctgattcttt ttttatcttc acaacaacca actttttctt
    23221 cagatccttt tactagaact tgtatacaaa aattgttgaa tgctgatata atttgcggct
    23281 acttgaaaaa acgatagatg taaaaagata aatagaatca ttttttattc tatatcagtc
    23341 tatgattcat tgcttatcaa tcacccaacc cattcgatgc cgtttttttt agagaaattt
    23401 aaaaaagacc gtacgaatct cgcattatcc gattactttt ccagaaaaga tccccaattc
    23461 tgtataaata accctatctt cttgttggtt tcattttctc atataagaat tcagtcaatc
    23521 tacccactat gaccattact acgtccaaca caactgacat attaaccacg taagtattcc
    23581 ctcacagatc tccgaatctc catggtatgg attcccatcc tgacgctgca gaggacaaca
    23641 tatttcatgt ggctctgtac ccttttgatt tggaaacact acaaatcaat actcatctat
    23701 aacttatcaa gcgaactttc gctaggcttc tagtgattac taaattgttg gataacatac
    23761 atagcccatt ttatctagct gttattcata acagttagtt cttgtattac ttcttccgct
    23821 tgtgttgcca ttgagcttgt caaaaagtta cttagaaaaa agctgccagt aaagcaactt
    23881 tcaacaaacc ctttttgttt cttatcatat caagaaacct ccttttataa attttttcta
    23941 taataaaaaa cgtcgataac ttaataagta tcgacgtaaa ctatttttat atcccgtaaa
    24001 tcaaaaattc taaagaaaga tttcctatat aagcatattg gataattcga ttagtattgg
    24061 gtatatctct accattctca tacctatcta tggaccattt tgaaattgaa ggagaaaaaa
    24121 gctttccaaa ctcctccctt tctagataat aatctttcct aattttcctc atccttaatc
    24181 ccatactaaa agaatttatc tttttaaaat cgctaactct ttcatctttt ggaagtatat
    24241 tcttataatc ttctccgtac ataaggtaag gaacagtgac cttaccaaga taggcaatat
    24301 tcattagttt ttcaatatct ggaatattaa tacctttttc ccatctactt ataacatttt
    24361 tgcctgtata gatggtaaag tttttaccaa atttttcaag cgtctcttcc cggttctttc
    24421 ggacttctcg tattcttttt cccataacaa ttttatcgtc ttttgataaa ctaaatatct
    24481 cactatcacg attaatctcc ttcaaaattt ctttttcatt acttaataga aaaatatcga
    24541 ttttttttgc atttcttttg acggatttct ctttctattt aacacataac tgagtaatac
    24601 ttcactaatt cctatattat cagctaactc tttgttaagt aacttattca tatctctgta
    24661 tgcaattagt ttaccgatga gaatctcaat cttatcttcc tcatctttta ttagagtttc
    24721 tatattcaga atcttgctta gagtattatc tacgtcttta gggatttttc ttgacccttt
    24781 ttcatattta actaaagtac tttgtgaaat acctgctaaa tcagctaact ttttttgaga
    24841 tagttttttc tcttttctta gagatttaag aacccttcca cttgaattat caaccatgaa
    24901 aaaagcacct cctatgagat gctttcatca tatactgaca gctaaaaatc ttcaagaaaa
    24961 atattccaat tttgttaatt ttagtatcgc cgtgataacc aaatgacagt ttaaaatctt
    25021 ttatacagat aatcctcagt gttgcatcta ctcaaaaagg tttatttaat aggtttttcg
    25081 attgattata agttatttca cactatcttg ctcatccatt ttcagaacgg ccatgaaggc
    25141 ttcttgcgga acttcgacgg agccgatttg tttcatccgc ttcttccctt ctttttgttt
    25201 ctctaagagt ttacgtttac gagaaacatc tccaccataa catttagcca agacgttttt
    25261 acgcaaggct ttgatgtctg aacgggcaac gattttttgt ccaatagctg cttggattgg
    25321 cacctcaaat tgttggcgtg ggattagttt tttcagtttt tcaacgattg cttttccacg
    25381 ttcataggca aagtctcggt gaacgataaa gcttaatgca tcgacttttt ctccattcag
    25441 caagatgtcc atcttcacta acttgctttt ttgataacca gacatttcat aatccaatga
    25501 ggcatagcct ttcgtgcttg atttcaattt gtcgaaaaag tcaaagacaa tttcagaaag
    25561 cggaatatta tagacgacat ttactcggta atcatctaaa taatccatcg taatgaattc
    25621 tccccgtttt cgttgagaaa gttccattac tgcaccgaca aaatcatttg ggaccatgat
    25681 ctgtgctttg acgaatggtt cttctacatc ctgaatcgtt acaggttcag gaaaatctgc
    25741 cgggttatct actgtagcag tcgtaccatc cgttttatta acatggtaaa taacggatgg
    25801 tgcagtcgtg atcaattcta gattgaattc ccgttccaag cgttcttgga cgacatccat
    25861 atgaagaagt ccgaggaatc cacagcggaa cccaaaacct aatgcttgag aggtttctgg
    25921 ttcgaattgt aaagcagcat cattcaattg cagtttttct aaagcttccc gcaaatcgtt
    25981 gtaacgagaa gtatcaattg gataaagacc acaataaacc atcggattca tttttcggta
    26041 acctggtaaa gcttcagcgg caggattgtc tgctaacgtt actgtatccc ctactcgtgt
    26101 atcttggaca gttttgatac ttgctgtgat ataaccgaca tcaccgacca tcaaaaagtc
    26161 tctagcaact gcttttggcg agaagacccc gacctctgta acatcaaatg ttttgccatt
    26221 actcatcagt ttgattttgt ctccgggttt gaccataccg tccgtgatcc ggacatttag
    26281 aacaacaccg cggtaactat cataaataga gtcaaagatc aaagctttta gcggtgcatc
    26341 taagtctcca cttggtgcag gaacatattc cacgatctgc tcgagaatat cttcaatccc
    26401 aatccctgat ttggcactgg ctaatacagc ttcactagca tcgattccga ttacgtcttc
    26461 gatttcttga cggacacgtt cgggatctgc tgcgggtagg tcgattttat tgatgactgg
    26521 caggatctcc aaatcattgt ctaacgctaa ataaacattg gccaatgttt gtgcttcgat
    26581 tccttgtgct gcatcgacca ctaaaacggc accctcacag gcagcaaggc ttcgagagac
    26641 ttcataggtg aagtcgacgt gccctggggt gtcaatcaag tgaaagatat atgtttctcc
    26701 atttttcgct gtgtaattga gttctacagc atttaattta atcgtgattc cacgttcacg
    26761 ttccaaatcc atcgagtcta acagctgatc ttgcatttcg cgagacgtca cagtgtgcgt
    26821 catttctaaa atacgatcgg ccaatgtaga tttcccatgg tcgatatgag cgatgatcga
    26881 aaaattacga atctgctctt ggcgttcttt cattttcttt atatccatag cgtctcctct
    26941 tttcatataa tcagcacttt ccattatacc aacgaaaagc agtaatttaa agagttttta
    27001 attagtcagc tatagaagtt ttttttgtag aattttgcta tactgttatc aagctaataa
    27061 atggagatga aaaaatggat caaaaagatt ttagagaaaa tcttgaatta aaaccggtca
    27121 ctcaagaaca tatcgatcaa ttcaatgaat tgctttccta tgtgttccaa gtgactgaag
    27181 cagacatcga ggaaagcggg tttgagaata aacgagcttt tatccgttcc aaaaaaccga
    27241 ttttggaagt atcgaaagtg ttcggatggt tccacgaaaa taaactgatc tctcaaatcg
    27301 ctatctatcc ttgtgaagtc aatattcacg gtgcacgtta taaaatgggt ggtgtgactg
    27361 gagttggaac ttatccagaa tatgctaacc atggattgat gcaagatcta attatcgttg
    27421 cattggataa tatgcgcaag aataagcaat ggatctctta tttgtatcct tacagtatcc
    27481 cctactatcg gcgtaaagga tgggaaatca tgtctgataa actttccttt aaaatcagag
    27541 atacacagct acctaaacaa atcccagtaa gtggtatggt tgagcgatta ccagttgatc
    27601 atccagacgt attttccgtt tatgacaaat ttgcccaaca aaaccatggt gctttattcc
    27661 gcagtgattt tcattgggaa gagtattggc gtttcgaaaa tgaagatgag cgcacagccg
    27721 ctgtctacta cgatagcaac catgaaccaa gaggtgttct gttctactgg gtagctgaag
    27781 aaatatttca cgtaaaagaa atgttctatc ttgatcaaga agctcgaaat ggcttatgga
    27841 actttatttc cgcacatttt tccatgatct actgggtaca tggagatatt tataaaaatg
    27901 agcctttagc atttttgatt gaagatagtc agatcaaaga gcagatcgaa ccatatttta
    27961 tggctcggat cgttgatgta aaagaattcc ttcaacggtt tccgtttgtt ggaacagctg
    28021 atgcttttca tttcatcatt gaagatcctg tcgctccttg gaataatggt gtcttcgctt
    28081 taacatggga tgaacaggga caagtccgtg tcttaaatga acctatagga aaatccgttc
    28141 gactggatat ccaaacattg acttgcttga tgatgaatta tcgccgtgct tcttatttgg
    28201 caagaatcga acggttagaa acagatgaag aaacactgaa gtcactagag cgtatcattc
    28261 caaacatgga agcttacttt agcgattatt tttgataaaa aggatgatgt ttagatgaaa
    28321 atttattttg ccgcaccaat gtttgctaaa agtgatttgt tatataacca acagttagtt
    28381 aaagaaatca gagagctctc ttctgagctt tcgatctatc ttcctcaaga aaatgaagca
    28441 atcaatgaca aaactgctta tgcagacagt cgaatgattg ctcttgctga cacagaaaaa
    28501 gtgttggcta gtgatttaat gattgcttta ctagatgggc tgacgatcga tgccggggta
    28561 gcatcggaaa tcggtgtggc ttatgcgaaa ggcatcccag tgatcggctt atatactgat
    28621 actcgacagc aaggcggaac ccacccgaaa aaaatcgctg cactacaaga aacagctgaa
    28681 aatcaatttc actacctgaa cttatataca ataggtttga tcaaattgaa cggaaaagtg
    28741 gtttcttcag aaattgagtt gctttcagaa gtaaaaagat ttttagatgg agggactttc
    28801 agtgattaaa gaaatcaaaa aggtgcaaat agctttactt gcttttggtg tattcgttgt
    28861 ctgctataat ttttatgaat ttatcacaca aaaatattcg acttctcaag gaatcacatt
    28921 tatcgtggaa tctttattag ggattgcatt gattttcatg ccacaagtca ttttgaaagt
    28981 tttcaaactt aaaataccag cagcaatcgt tttgttttac tggtttttct tgttcatctc
    29041 tgtcttttta ggcacaggga tgcatttgat cagtatcatc tctttttggg acaaaatctt
    29101 acatgcggtg agtccaatgg tcctgacagc tcttggctat ggattgattg gttacttgat
    29161 gaaagatgct gagatatcaa aaaccagtcc ttggttgttc ctcttattcg gctttgcgtt
    29221 tgctggactt tgcggcgtgt tttgggagtt ctgggaattc ttatgcgacc agttcttagg
    29281 catgaatctt caacgttttg ctgcttctga tggcacactg tttgtcggac gagcagcatt
    29341 gatggatacg atgggcgact tgctgacaaa tacgataggt gctgccttaa tgggattgtt
    29401 tgcatggtct caaagtaaaa aagatgagcg ttactttgaa agttataaat tagaaaaagt
    29461 gaaaaatacc taataaaaga ggttgtgaca gaagcgggca gcttcaagaa atttcggctt
    29521 atttttcgaa gaaactactt ctggaacact gtttatttga aaaaaggagg tctgacacta
    29581 gttgtgtcag accccttttt tcttttatag tctcaatgga agattagacg atttcttcta
    29641 gctcttctgt tttgaaatga agttgtccat caagtaaagt aatcgttact tttgatttag
    29701 gcataacatg tcctgcaacg atttctttag ctagtggtgt ttctacttcc ttagtgataa
    29761 atcgtttcaa tggtcttgca ccatatgctg gttcataagc attttcagca atccacgttt
    29821 tggcttcatc actgattgtc agcaggattt cttgatgttc cagacgttgt gccaattgcg
    29881 ccaccatttt gtcaacgatt cctttcacgt tgtctaggct caatggtgta aataaaatcg
    29941 tgtcgtcgat tcggttcaag aattctggtt tgaagtttcc acgtaatagc gtgttgactt
    30001 gttcagccac tgcttctgga atcgttccat cagcagtcac gccttccagc agtaactgtg
    30061 aaccgatatt gctggtcatg atcagaaccg tatttttgaa atctactact cgcccttttg
    30121 aatcagtcaa gcgtccgtca tctaggactt gtaacaagat attgaagaca tcgggatgcg
    30181 ctttttcgat ttcatccaac aagacgattg tataaggatt tcgtctgaca gcttccgtta
    30241 attgaccacc ttcttcgtaa cctacatagc ctggaggagc tccgactaga cgagacacag
    30301 catgtttttc catatattca ctcatatcga tccgtaccat atgatcttct gagtcaaaca
    30361 agttctcagc taacgctttc gctaattcgg tttttccgac accagttggt cctaggaaaa
    30421 ggaaagaacc tagaggacga tttgggtctt gcaatccagc ccgtgaacga attaccgcgt
    30481 cactgaccgc atcaactgct tcatcttgac caatcactcg tttatgaagt gtttcattca
    30541 atttaattag tttttctcgt tcaccttcta caagtttcgt tacggggata cccgttaatc
    30601 tgccaacgac ttgagcaatc tcattttcgg taacggactc ttgaaccatc ttaatctcgc
    30661 tgtcttttgc tttagcttct aattccttca attctttttc taattgaggt atcgtaccat
    30721 gacgcaatac agcagcgcgt tctaaatcat agttattttc tgcatcttct aattcgtgtt
    30781 ttgctttatc gatttcagca cgttttgctg aaacagaatt gacttcttct ttttctgttt
    30841 cccactgcat cttcatcgca tttgcttctt cacgaagttc agctaattct tcttgcaaat
    30901 ttttcaagcg ttttttactt gcatcatctg attctttttt cagtgcagct tcttcgattt
    30961 ccaactgcat caaacgtcta gtaacttgat ctaattccgt tggcatagag ttcatttcta
    31021 cccgaatcgt tgcactggct tcatcgatca aatcgattgc tttgtccggt aagaaccgat
    31081 cagtaatata acggtcagat aaagtagctg ctgctactaa tgcgttgtca tggatgttaa
    31141 caccatggtg gatctcaaaa cgttctttta atccacgcag aatactgatc gtatcttcca
    31201 ctgttggttc tttgactaat accttttgaa agcgacgttc caacgctttg tctttttcca
    31261 tatattgacg gtactcatct aaagttgtgg caccgatcaa atgtaattca ccgcgagcaa
    31321 gcattggttt caacagattt ccggcatcca tacttccttc tgtttttcct gcccctacga
    31381 tattgtggat ttcatcgatg aacaagagga tacgtccatc actttttttg acttctttca
    31441 agactgcttt tagtctttct tcaaattcac cacggaattt tgctccagcg atcaatgcac
    31501 ccatatctaa tgaaaaaatc gttttatctt ttaaattttc aggcacatct ttgcgtacga
    31561 tacgttgagc aagcccttca acgattgccg ttttccctac accgggttct cctatcagaa
    31621 caggattatt ttttgttttt cgtgaaagaa tacggatgac atcacggatt tcttcatcac
    31681 gaccgatgat tggatccatt tttccgcttt tgacttgctg tactaaatca acgccgtatt
    31741 tttctaatgc tttgtattgt tcttcttggt tttgtgaggt cactctttct cctcctctca
    31801 tctcttcgat gtttttacgc aattcttttt cagataatcc gtttttattc aaataaacag
    31861 ttaacggata attttttaat ttcatcaacg ctaaaataac gacttccgtt gaaagaaact
    31921 cgtcgccgaa gctttcacgt atttggtctg cttcattcaa taatcgaaag aaattctggc
    31981 tgaggttttg tccatattgg acgtttccac cagaaacaac tggatactca tccagtagac
    32041 gatcaatttc atgttcaaaa gattcgacat ccaatccggc atccgtatag aaatttcgac
    32101 caaaatgatt agattgtaaa aagattttcc atacatgggc aatgtcgata tcttgatgtt
    32161 ttcgagtgac cgcaatctgt tgagcttcag caatcgcttc ttgaagcgtc gtagtcattt
    32221 tctcaatatt catacaggta taccctccta attaaaggta agatcactta tttctttgat
    32281 ctttacaaaa actattatat cgctttggtc aattttggtc aaaatataca catagttttt
    32341 tgactttttt tctcgactta tataaatctt aaaaaaacaa aattgattgt atgtatataa
    32401 aaaaagagcc aaacaaacgt aaaattatct tatccataac gtttttcttt gatttgataa
    32461 aaagggtaga tcttataaaa gacctaccct tgattttctt tattctcttt ttttacttct
    32521 attatttctt ctttgatcac tcttcgccgt tcagcaatca catacacaaa tccatagaga
    32581 aatggcaaga taaaggaact gaaacgatac agtaaaagcg aagaaactgc gtcaactgtt
    32641 ccaacaactg gtctgaataa taacaaatag acaaattcaa aggaaccaat accagcaggc
    32701 gtaggaataa cccctgaaag cactactgca aagctgataa acgaaatggt cagtagaaaa
    32761 tctattttag gatggttttc aatcagaatc aaataaggaa gtacatacca aaatacaagt
    32821 tttaacatat tccatagata aatccgcaat agcgcagtcc tgtcttggat aatggtttgg
    32881 accgtttccc ttaaagagta gatctgctga ttgcatgtat ccacccattt ccgcaaacgc
    32941 tcacttttaa ataacttatt cgataagctg acaaaaaata tttgaacatt caagctgacg
    33001 gacatcgcca ataaaaaggc aacaatcacg atcgtcaaaa ggataccagc gatgataaac
    33061 cagatcattg atggaccatt agaataaaaa agagagaatt ggatgaccaa gccagctaat
    33121 gcataagtga tcactgccag tttgtacata atcatatgga gtgctgtcac ccctgctccc
    33181 tgggatagct tcattccttt tttgttataa aaataaatct ccgagatcag cgttcctgta
    33241 ccaaacgaaa cgattcggta aaacgcaata tagctagagg tcaagaaccc atctttcgtt
    33301 gtaaaattcg gcgcgaaagg ttcagcaatt tctttgattg agcgcccttc agcgatttgg
    33361 taaactgtcc ctaatataac agccgctgcc aaaacaacaa cactggtaga cagcaattcg
    33421 gcaaagatat ctgcaaatga ttgattgatt aaataataaa taatggcaaa aatcacaatc
    33481 agcaaaagca gattcagaat gattttagtt ttcgttcctt tagacgacat ctcctgctcc
    33541 ttaatggaaa aaataaatga gaatcaaatc aacaagtaca cctgcagcga atcctatagt
    33601 aaacttcttt ttccgagttt tatgatggaa aacttttcga gagatcaatc ctcccaaacc
    33661 accgcctaac aggctgacaa atatcaaatt attctcagga acacgccagc cgctagttct
    33721 agcctgatgt ttatcatacc ccatcagaca aaataagtaa aaattcacaa tgaaaaggta
    33781 aaaccacaaa ggattgtgta acaatacatc cattactctt cttccaactt tctgttaact
    33841 actatttcta agctttttct tctttctttg gtgaataaac tgttaatcct tttgatacaa
    33901 cagataattc aactggaaga tcatctgtcg tgtctccatc cgttcgagtg cccacagatt
    33961 tttcctgcgc ttggattttc atgcgtttgg ctgtgaaata agtaatgccg gggatcgtat
    34021 agaatttgcc tctcatgatt ctaggcaaat aaaaaataaa tcggaaaata tctaaggaag
    34081 gtacgatcgt tacatgaaat tttccatcat ctagggtcgc tgagtcgtca aaatttgtaa
    34141 aaccgcccac ggagttagac atcgtgactg tcaacagttg ggattttcct ttccaatgct
    34201 cctcgtctgt ttctaaatcc aaatgatatt ttttcttctt cagcaataac ttaaaaaact
    34261 tcttgataaa gatccacgag ccgtaacgct gtttttcttc ttgactgatc caaactgctg
    34321 tatctgctaa gattccgatt gtcagtgtag agataatgac ttcatcattg acttgaccat
    34381 agtctatttt ccgtacattg ccttccagga tcacgtcagc agcttcttct tttttgaggg
    34441 gaatctcaag aacacgtgca aggttgttga cagttccacc tggaagtaat ccgacatggt
    34501 attgagataa tgttttttgg aacgcttgga cgatatgatg aatcgtgccg tctccaccta
    34561 taattactaa agtatcagct tctatggctt ctgctttctt gactaattcc gcatcatcaa
    34621 tcgatgggcc agtttcagct agttctacat ataattctgg ctgcttttct tttacataat
    34681 cttgaaacca gcgtgctaat ttctcaccct catctttccc tgaacttttg ttataaaaaa
    34741 ctagcagctt ttccattctt tacctcctat ttactcttca aaaaagaggg cgggacaacc
    34801 aattccattg tcctgccctt tagccttaga cagatgacat tccatcattg ggacgaacgc
    34861 cttgtcataa gctgttcttt ttcgatttta actctttgtg ttcaattctg caatttttac
    34921 aatcacgtct gttgcttttt ccattacttg taaagaaaca aattcgaatc gtccgtgcat
    34981 attttctcct ccggcaaata gatttggcgt cggaataccc atgtaagaga tttttgaacc
    35041 atctgttcct ccacgaactg gttcgatcac tggagagata ccaagctcga tcattgcttg
    35101 tttcgctaat tccacgatgc tcatatcttt ttcaatgatt tctttcatgt tgtaatattg
    35161 atcatacata tctactttga ttcgttcttg atcaaatcgc tgattcaact cttcttggat
    35221 atgtgtgatt tgtgctttgc gtgcttcgaa tttttcacga tcatgatcgc gaatgatata
    35281 actcatagaa gcttcttcag gattcccatc caatgccatc aaatggaaga aaccttcgta
    35341 tccttccgtt ttttctggaa cttcatcggc tggaagctga ttatgaaaat cgattgctaa
    35401 ctgtagagca ttgatcattg tatcttttgc cgtccctgga tgtacatttt tcccttggat
    35461 cgtaatattt gcttgagcag cactaaacgt ttcatactgc aactcaccta aaggaccacc
    35521 atcgactgta taagcaaaat ctacatcgaa ttgctcaaca tcgaatttat ccgctccgac
    35581 accgatttct tcatcaggac caaaagccac tttgatggta ccgtgaggga tagaaggatt
    35641 attgatcaag atctccattg cagtcatgat ttccgcaatc cctgatttgt catctgctcc
    35701 cagtaatgta ctgccatctg ttgtgatcaa tgtgtggcct tcataatttt tcaaatttgg
    35761 aaaatctttt gtattcaatg tatagcgttc ttccgcatct agtacgatcg tagactcgcc
    35821 atcatagttt tcaatgattt gtggattgac tcctactgca ttaaaatctg ctgtatccat
    35881 gtgggaaata aatccaatgg aacgcacttc atgatcaaca ttgcttggta atgttcccat
    35941 aacgaatccg ttatcttcat tatagaaaac atcagctaag cctaactctt ctaattcttt
    36001 tttcaattct tgcgcaaaag caacttgtgt ctgagtcgat ggagtcgttt tgcttgttgc
    36061 atcggagcgt gtttctgttt tgacatatcg taaaaaacgt ggcaaaagat tttcgtacat
    36121 agtattctct ccttttaatt aaattgaaat gggtttgtat ttgtttcaga aacgaaaaag
    36181 ttaatatccc aattttcttc ttgtttccaa gattcaaatt tctcgataaa tcgttgcttg
    36241 cataacgatt caatataatg accgggatca attgcaatca acccagcgct ttgcatatca
    36301 tgagccgtat gataatagat atctcccgtg atatagacat ctgctctttg ggcgattgct
    36361 tgcggataaa atttttcacc gcttccaccg caaatcgcaa tgcgcttcac agaggatttt
    36421 gcattttttg gctggactat tctcagtcca tctagttgga acgcttcttt tacttgttct
    36481 acaaatgcct ctatagaaat ttcttgcggc aattcaccta ctcgtcctaa cccaaacatt
    36541 tcgactggtt catctatagc gaaaagatca taagcaggtt cctcataagg atgagctgac
    36601 cgcatggctt gaatcacttg tttttcgata gtttccggca aaataacctc aacttttgcc
    36661 tcttgaactt gttctgtctt ccctactttc ccaatcgctg gttgtgcacc agcttccgga
    36721 gtaaatcgac cgtgtccaat cgaggtaaaa cttgtaccgg tataatcacc ttgtgtacca
    36781 gcaccagctg cagctaatac ttcacgcatt ttctgtgcat gatccacagg tacataaacg
    36841 gccagttttt tataatgaat ctcatgagtc tttactaaat aactctccac ttcaataccg
    36901 agaagttcac aaaaccagtc attcagtcca tcccaaataa tatccatgtt cgtgtgtgct
    36961 gcatagacgg caatatcatg tttcaacaaa tctgcataca ttttttcttg tggctgatca
    37021 gtaaccagcc gttttacagg acgaaagatt ggaggatgtt tagcaatcag caaatcgatt
    37081 tttttcttga ttgcttcttc gaccacttct ggacgaacgt ctaatgtcat cattactcgt
    37141 tggattggct tatccaatgt tccgatatgc agtccgactg ggtctccttc ttcagctagc
    37201 cactgaggac aatagctttc aaacttttgg atgaaagtgc gtccgctgat tgtcatggct
    37261 ttaaaacctc ctcaatcatt tggatttcct gctcgatttg tttttgacgt gcctcttgat
    37321 tagctgcttg tttgagctgt tcacggatag cttttctttg ttgaagctct cttgtccatt
    37381 ttgatttgaa gatctcactt ttctcttcaa gcagaaatgg cccgaacatc agctccattt
    37441 cattgtaagt aactggtttt tgttcttttt cggctacaat gatttcatat atttttttat
    37501 tttcttcaag tattttttct gattggattt tatactgatt ttcttgcagc caagatcgta
    37561 atgtacgctc tccgatgttt ggctgaagga tcaaacgttc ttgtccgctt aagcgttgat
    37621 tcaacttgcc agcttctagg atatctcgga tcagtgaacc tcccataccc gcaatcgtga
    37681 cagctgtaat accatcttgt ggctggatag catctagacc atttgcaagg cgtacggtga
    37741 tacggtcttc taatccatct tttttgactt gcttacgtgc tgattcataa ggaccttgaa
    37801 cgacttcacc agcaactgca tattcgattt ttcctcttaa tatcaatgca acgggtaagt
    37861 aagcgtgatc tgatccaatg tctgctaaac gagcggattc agggacaaag tcacccactg
    37921 cggctaagcg ctgagataaa tctaaatggt tcatagcttc actccttcta gataattata
    37981 gcattttcca aataaaataa ggagactagg aataaaaaaa ttcccatatc tccttaaaaa
    38041 atataccttt tcgttctata aaacaacatt ttcttatttt tgaaaaaatg ttattggtta
    38101 tttgttcaat gaaacatcaa taatcgaatc tccaggaact tgcggtggga tagacatcgt
    38161 catgatcggc atctcaacta atgtaaattg gactttgtca ccgattttca attcatttat
    38221 gtccacgctt attttttcag cagtagcatt taagatcact ccgtcattgc tgaatgacgt
    38281 tcccatattc gctggatctt ctttggccat gacatcgacg agtttcaact gaagcgtttc
    38341 ttcttctgct tctacttgag tgatactttc aatcgttccg acaaatatgc tttgtctctc
    38401 cgttttcgta ctgcttgttt ttactgtttg acccgcatca atcgaatcaa cagaacctgt
    38461 gttgctatct gcgctaccgc agcctgctaa aagaaaaaat agaccaacta cagatatact
    38521 agaaaaaata gccctcataa aaaatcctcc gtttaagttt ctgcattact tgttgcggtg
    38581 attgtctccg gtcatttctg tccatataat gattcgctga cctgctttta ctactaggtc
    38641 aaatgtcagt tggacttgtc aactgttcaa ccattgcgtc ggttgcgacc agtcatttcg
    38701 atttcttttg tcaaataacg tatgcgttcc attatccgtt tcgcttttag cggctcttca
    38761 tctccacgtt gagtgacgga caaatgctgt tccaattcat ccattgtaaa attcgatgag
    38821 aagaaggttg gtaactgttc ctgcatccga tattgaagga taacaccgaa tacttcgtct
    38881 cgtatccaac tactcatcgc atctgcacca atgtcatcga tcatcaaaat aggtgaacgt
    38941 ttcactgcat ctaacttttc tgccacttga tcttttccga ttgcctgttt catctcaacg
    39001 gcaaatgtcg gaaaatggac tagcgtcgtg gtgaatccag caatcgctaa ttctctcgct
    39061 gttgctccta ataaatatgt ttttccaata ccaaaacttc ctactagata cagtccttta
    39121 tgataccctt ttggatcagc agtatatttc tctacaaaat ctagggcttc taagctcgca
    39181 tatcctcgtc catctgtacg ttcaaagttt tcaaaagaag ccgattgaat atcttttggc
    39241 atatccattg cttttactcg gcttctgatt tcttcttgtc tttgccgtgc taacaatgct
    39301 tccgttggta cataagtgac atccacataa tgaaaattca acgtcagctt cggctcatag
    39361 ccgggagcga tcatcgttgg atcgtttttt tgatatttac gcttttcttg cacgaattca
    39421 tacaatttag cgtaactttt ttcaatatcc gcttgtgtca attcttcttg gtgtgcgctt
    39481 agaaaagctt gaacatccgc atcatttaag acttcagcga tcatttcagc atacttttct
    39541 tgataattgc gtttgctgat gatcctcttc aattctttgc ctacgtcttc cactagatat
    39601 caccctcctt ttttgccagg tattctttaa aacggcggtc aatctctgct tttttctcgc
    39661 tgctgatctg tttttcatct tttggctggt tgacccaatc aggaagcttt tcccgtcgaa
    39721 catttcgctt gccagtttga taatttgttt gcttcttttg ttttgattgt tttgcttgat
    39781 tacttatttg ccgaatgtgc tggatcgctt cttctgctgt ggcgattttc ttttgtgccc
    39841 attcatttgc cactgtattt acataactgg catttaagct agcattattc ttgatcacta
    39901 gtacataatt cagcaaaaca ttgatcacgc tactggaaag acctgattga gaaacaagat
    39961 cttgcagtaa ccagttttct tgtttagaaa catatccgcc tttttctgac ttgattgctt
    40021 ccagatattt catcggtgga atgctctgtg cttcttttat cagctgaagc gctgcgtttg
    40081 tcaaatgttc gtttgacgag ggttcattac tgttttcaga aaccgcaact gtttcttttg
    40141 tttgcttcac cggacgtctc tgcattgcca aaatggcttt ttgcagttct ttttcagata
    40201 ctttttgttc actaaagtca taagattgac tgatcagttc gccaagtgtc aattcatcga
    40261 aaccgtaaag ctgatgatac atgtcgactt gtttgatgat tggatcatca agacttgcaa
    40321 tatgtttttt tgccagccaa tcattcaaaa actgccaatc gaatgtcgaa tctgcttcaa
    40381 gtaatgctac tgattcagat tcttcaaaag atttattcag cgattcgatg ttttcttgat
    40441 tagcagcgta cactgcttca ttgaagcgat aaacatctaa aaattttttt gtgatttttt
    40501 gatatccctc tgctgtatgc cttttaggtt caaagaattg ggtaagcttc tggaatcgtc
    40561 gttcaccaac tttttccaga agaagaaaag ccagaacaga gtctttgaaa aatgctttag
    40621 gaatgagtgg ttctaacagc tcataaataa aacgaggtcc cagatcacta tcttctttaa
    40681 aataaacatc cagtaagccg attccttcta gcttttttct tgcatcataa aattgcggga
    40741 taccgcagcc tagactacta agcagatctg catgcatccc ttcaggacct ttgccagatt
    40801 cttgagagat ttctgataac agggtgaaat agatactcaa ggcttctccg ccgattagtg
    40861 gctgataaag atacagcaag gcgttcttgc tgtcttctct cagcggagcc gcttttcttc
    40921 cttggtaaat attttttggt tgaagctctt tccaagcatt ttccaaggaa atgcctcctt
    40981 tttgtttctt aattgttttc tttattattc tctttattat tttcttttgc tttatccacg
    41041 atatcttgca actcttttaa gaaaacagac atatctttga actggcgata aacacttgca
    41101 aaacgaatat aagcgatttc gtctaaatca actaaatctt ccatcacata ttcaccaatt
    41161 agattcgtcg atacttcgtt ttctccgaga ctgcgtacgc ggttttcgac atggttgacg
    41221 atttgttcca tttgttccat agctacaggt cttttttctg ctgaacggat gagtcctctt
    41281 aaaattttat cgcgattgaa ctcttcgcgt tccccgtttt tcttgattac taataacgga
    41341 gcagcttcaa ttcgttcaaa tgtagtgaat cggaacgaac aattctcaca ttcgcgacgg
    41401 cgacggatcg cacgtccatc atctgcttga cggctgtcga ttactcgtga attattatgt
    41461 tgacatctgg gacatctcat tttgtctcac ctcttgcatc gcattttata taaaggatgt
    41521 gtctttcttt acaactagtt gtattataac acgaaacaaa tcacaaacgc gactgtccta
    41581 cccaaaaaac acggatataa gaacaatttt ttctactttc atgcgtaatt gtgtcccctg
    41641 tccttactaa atttcaattt tccaatactg atcaccttga ttactgagaa ataacttaca
    41701 caaaagctga tttagtcatc tagcacaaaa aacttgaagc agatgaacac cttctcatct
    41761 gcttcaagtc acatctcgat tttttactat tttataactt ctcttcgatt cctgtatagt
    41821 gtttcattcg ctgttcctcg attttttgct catcgggaat gtggtcactt tctgggagcg
    41881 gtgttttact acgatcaggt gtcggatttt ttcgttcttg agtcaaatct tgccgcttcg
    41941 cttcttcttc tgcctgctgg tcttctattt catcgattct tgcttggtac tgcttttctt
    42001 tttcttctgc tgtttctgat tgcgtacgac cttcatcgat tagattttct ttattgagca
    42061 tctcgccgat tttttctttt gcgttaccta cgactttatc cttttgattt tttaatgatc
    42121 ccatcgctat ccctcacttt ctatatactc aggatagcaa aattgacaca gatactcaaa
    42181 gaaagcgatt agcaaaaagc caattttcga tttgtcccac aagtatttct tttgttccat
    42241 tgttgtcgaa caaaatatcc gcacgctgct ttttttcttc gattggaagt tgactactaa
    42301 ttctctgctg cgcttgttct agcgtcagtt gattacgttt catcaaacgc tgaagctgag
    42361 tttctggcgt agtataaacg acagctactg catccatata gtggtcataa tgaccttcgt
    42421 agagcaatgg gatatctaca attactaaag gatgattgga acttagagag gcgatttgat
    42481 cttttatcgc ttttcggata aacggatcta atagctgatc aagtttttgc cgtttttcct
    42541 ttgatggaaa aacaatcatc ccaagtttcc ctcgatctag ttgcccatca ggctgcaata
    42601 cctcttggcc aaacactgat acaattttcc tcagagcaag cgttccaggc tcaacagttt
    42661 ctctggcaat cacatcgcca tcgatcaaag gaaatccatg agactgaaag actttggctg
    42721 ccgtactttt accggtagct attccacccg tcaaacctaa aacaaatcct actttgcgat
    42781 ttttagcctt tgacaattcg gacaatagtg cgtccccctt tgtgccactt ttgttttcac
    42841 gatcggcgtt ccacaacgta tacatggttt gccagtttgc tgatagacat gcagtgctac
    42901 ttgaaatccg cctgattctc ctaatgcatt caaataactg cgaatcgtgg tacctccggc
    42961 ttctattgca cgatccaaaa catcaatgat cgatagacgt aattgttcta cctctttggg
    43021 gcgcagtgtg ttcgccggtt gttctggatg gatcttcgct tcccataatg cttcatctac
    43081 ataaatatta cctagaccag tcactagacg ttggtctagc agcaatggct tgattgcttt
    43141 gtgggacttt ttcagtccat ttgcgaaatc tgctaataag aagctatctg gcaaaggctc
    43201 aggtcccaat ttcatgattc ctcggtaagt tgcacttgct cctttttcga cgattgtcat
    43261 acgaccaaat tttcgtacat cgttgtagcg aagttggctt ccatcctcaa aaaagaaaag
    43321 tacatgcgta tgtttatcga ttggtgtatt ttctttcgta aattgatatt ttccttccat
    43381 tcgcaagtgt gagatcagtt cacaatgatc gagatgaaaa attaaaaact tccctctacg
    43441 tccaatggat tggatggttt cgccaacaag agaagcctca aagatgggtg tctctggttg
    43501 ctcgatgatc ttgggccaca agacctgaac tttttgaatc gtttttccta ctactaaccg
    43561 ttccaatcct ttacgaacgg tttctacttc tggtaattct ggcatttttt cttttcacct
    43621 tcattatatt gctctgtctt aaacaatgtt tattttgctt cgtaccacgt ttttccccaa
    43681 ctgctgtcag tgatcagtgg tacatgaaga gaaacagctt gttccataac ttccttcacc
    43741 aatttatcta atgattcaag ttctttttcc ggtacctcga agacaagttc atcatgaact
    43801 tgtaacagca tcgtagcttg aagacctgtt tcttttaatc gtttatccaa ttcgatcatc
    43861 gcgattttca aaatatcagc agcacttcct tggattggtg tattgatagc tgttcgttcc
    43921 gcaaaagaac ggatattgta atttcttgaa ttgatgtctg gcagatatcg acggcggtga
    43981 tacagcgttt cgacgtagcc ttggtctttt gcttcgcgta cgattctttc catatattct
    44041 ttgacacctg ggtatttttc aaagtacgta tcgatgtatt gctgtgctgc ttttcggctg
    44101 attcctagat tttgtgaaag tccgtaatca gagatgccat acacgatacc aaaattcact
    44161 gcttttgcct gcctacgcat atttggtgtg acatcctctg ccttttcgat tccaaataca
    44221 cgcatcgctg tgctggcatg gatatcttga ccttctagga aggcagcttt caaatgttca
    44281 tcatttgaga tatgcgcaag tacgcgtaac tcgatttgtg aatagtcaga agaataaatc
    44341 agccagtctt tttctctagg aacaaatgcc tgacgtatct ttctcccttc atctaatcga
    44401 atcgggatgt tctgcaaatt aggatcaaca gagcttaatc gtcctgtttg ggtcaatgtc
    44461 tggacataac gggtatgcac ttttccatct gattggatca ctttcagcag tccctcgaca
    44521 taggtagatt gaatttttgc aatttgacga taggtgagga tatcctctac gattggtgct
    44581 tgttcacgaa gctgttccag tacatctacg gctgtggaat atccagtttt tgtttttttg
    44641 ataacgggta atcccatttt ttcaaataag atcactccta attgctttgg tgaattcaaa
    44701 ttgaattctt cgccggcttc ttcgtagatt ttttgttcga tctctcttaa gcgagcagag
    44761 aactcgccct tcatttcttg taagcgagtc gcatctactt tgatcccgct tatttccatc
    44821 tctgccaaaa tgatagacaa tggcagttcc attttccgga aaagatcttc ttggtttttt
    44881 tctgccagtt cttggctcaa ttttggtgtc aacgcattga ttgcagctac ttttcgcgct
    44941 aaatgagcaa aaaagagttc ctcttcttct ggtaaacctt ttttcgcccc tttgccgtat
    45001 acagcttcat ctgattggat atcgttgtat ccgtaatgag cagctattcc ttcgatatct
    45061 gtgctttttt cattcgtatc gagaaggtat gcacctagca agacatcaaa atcgattcct
    45121 ttcgtttttc cagtatagcg gtttaaagca acataagttc gtttcgcatc atatactttt
    45181 ttcagccgag tcggatcagt gatccaagaa tggaacgctc ggttttcgaa tacagataga
    45241 tcgttcgtga cataaatttt cttttctgtc ccccacgcta ctccgacgat ttcctctgta
    45301 tgataattgt cgcccatcat ttccacgtac aacgccatat ccgtagtaaa catttcctct
    45361 ttgaattcat gaacgacttc aaacaaaata tcatccattt ttacttcttc ttctacgata
    45421 ttcaatttag aaaggaactg attgaaatcc atttctttat aaaacggaac gagtttatcg
    45481 agatttttcc cttcatactt caatgagtcg atagagacat ccacaggcgc tgaagtattg
    45541 atggtcgcca attttttcga taagaaagcc tgctctttgt catttatcag attttctttc
    45601 attttgcttt gcttcatttc atcgatatgt tcataaattc cttcgactga gccatattgt
    45661 ttcaatagct tgatagctgt tttttcaccg attttagtca caccggggat attgtcagag
    45721 gcatctccgg ctagtccctt catgtcaata atctgttttg gcgtcaaccc atcatatttt
    45781 tccgctacat gttctggtgt atagctttcg atatcgctga caccttttac agtgatatcc
    45841 acttttactt ctttactagc taattgagtc aaatcccgat cacctgaaag cacgacgaca
    45901 tcaaattgat ctttgtctac tttttctgcc aaagtaccga taatatcatc tgcttcataa
    45961 ttgggcaatt cgtaatactg tacccctagt cctgtcaaca aatcacgtat atatggcatt
    46021 tgttctttga attcgccagg tgttttggat cttccacctt tgtactccgc atacatttct
    46081 gtccgaaaag tcgtttttcc tgcatcgaac gcaaccagaa catgagtcgg ttgctctttt
    46141 tgcataacat tttcaaacat cgtattgaat gcatagattg catttgtatg aagaccgttt
    46201 ctatttttga aacgttcaag agaattgtga agcgcaaaga atgcgcgaaa agccacactg
    46261 ttcccatcaa cgagtaataa tttatttttt gtcatcttct taactcctta ctgtaaagtt
    46321 cttattcatt ctaacaaaga atcctgcgga atgcgaaaag aaaaagcggg accataccaa
    46381 ataattcggt atagccccaa ctgatgaatt tttttctttt ttagttgttc cgtccaaaga
    46441 ttctcagaaa cgccaagaac aagttgataa agtccaaata cagcttcaat gccatgaata
    46501 cagcaattcc actatgtgct gttccgtttg tctggacata gatattacgg atgctttggt
    46561 tatcataagc tgtaatccct gcaaagatga cgactaacag tagggagatg aacagctcaa
    46621 ctacgctact tctcaaaatg aagatgttca agactagtgc aatgatcact ccgattaaag
    46681 ctgcctgtcc tgctcttcca accccgctaa gatctctttt tgttgttact ccgacaacag
    46741 acatccccat gaacatcaaa gtagctgtca agaaagcttg cgttactgtt ccaatatcgt
    46801 agtatgcaag agtaaccgct aatattatgc cattcaaaag agagtaggca ataaatccac
    46861 cgattgctaa gctcggattc ttttgtgctt ttacgcctag aaccattact aaaatcagtt
    46921 caacgatcca cagacccaca aagcctagtg gaaaattatt gataaaagtt gctaattgca
    46981 aaggaaatac ccaaagagcc aagtaagaag tgactgcact aagggcaagt cccataccta
    47041 aaaatccata aacttttgca taaaaacgat tgatacctga cattgatgta cttggattat
    47101 tcattcaatt caccttcact ttcgattgct atttccaatt caggaacttg tggcggttgg
    47161 accataacga cagcatccaa cacacgatct tcgttcgtat tctccgcttc tacagaaatc
    47221 gtcaccacat ccttcatctt atctactttt atgacctcaa actgaaaagt cagtgtttca
    47281 taatggaaaa ccggttcaat aaaattgaca gaaaaattga caacatgtga tcctggtcca
    47341 ggaagatgct ttgaaattgc acttgtgatg atccccatca gcatcactga tggaacgatt
    47401 ggctgttgat attctgtttt ttgtgcaaag tcatgttgga tatacaaagg atttgcatca
    47461 ttcgtcaatc ccaaatacag caaaagctgg ttatcttcaa tagattctgt taaggataac
    47521 gagtcgcctt cctcgatttc atttattgtt tttcctagtt ttctcggctt acctatgttc
    47581 aagatagaaa ccctccagtt tatttttaat catacattat tgtaccaaaa gtagcgtaaa
    47641 aggcaagttc tgctcccttg atttaacaga ttcctatagt ttacagaggt cgtgaaaaag
    47701 ctgtcctgca tcaaataata agaacagcca agaaaaaaca gcttctcatg tttttgattt
    47761 gattgggctt attaccgcag atgcaagctt ttgaacaccg tttatcataa gttgcaaaaa
    47821 agggggcttg gcaagatttt tgccactccc cctctttcta tttttataag gacactgcag
    47881 taattgtatt acagtacatt tttcgtcgtg ctgcttaaaa gtttttcata cgcttgttca
    47941 aatttttgaa catctccagc acccatgaag attactactg catcatgata atctaacaat
    48001 ggagatacat tattttcttt gatgacttct ccaccttttt tgattttcgc tccaaggtct
    48061 tcaatcttaa cgtttccttg ttcttcccga gccgaaccaa aaatatcgca taagtaaact
    48121 ttatcagcta aatcaagtgc ttctgcaaat tcatccatca atgcaatcgt acgcgtaaac
    48181 gtatggggtt ggaaaactgc aattatttct ttatctggat atttttggcg tgcgccatca
    48241 atcgttgctt tgatctctgc agggtgatgc gcataatcat cgactacggt catgtctgct
    48301 acgatctttt cactgaatcg acgttttact cctgggaaag tcagcatttc ttctgcaact
    48361 tctttcagat ccagtttttc gaaataagct actgcaataa cacctaaagc atttaagata
    48421 ttgtgtttac cgaatgctgg tacagtgaag tgcccaacaa attcatcccc atgatagaca
    48481 tcaaaagcag aaccagaagt tgtgcgttcg atgtttcgtg cttggatatc atcgttttct
    48541 gtgacaccat aataataaat cggcacattt gcttttaatt tgcgtaaata agcatcgtca
    48601 ccatacgcaa aaatagcttt tttcacttgt ccagccattg tttggaaagc tgtaaataca
    48661 tcgtcgatac ttgtataata atccggatga tcaaaatcga tattggtcat gattgcatag
    48721 tctggtgaat aggcaaggaa atgacggcga tattcacagg cctcaaatga aaagaattcc
    48781 gcttgcggat caccatgtcc tgttccatct ccaattagat aacttgttgg acggacacca
    48841 ctcaatacat gagagagcag tccagtcgta cttgtttttc cgtgagagcc tgttacagcg
    48901 atactcgtat aattttggat aaaatgacca ataaagtcgt gatagcgaat cacttccaaa
    48961 cctaattctt ttgctcgctg gatttcttca tgtgaatcag gaaatgcatt tcctgcaatg
    49021 atcgtcatcc ctggttttac attatctgcg ttaaatggca gaatcgtgat atttgctttt
    49081 tctaaatctc tttgtgtaaa gaaatatttt tcaatgtctg atccttggac atttagacct
    49141 tgttcatgta aaacaagagc caaggagctc attcctgaac ctttgatacc aacaaaatga
    49201 tacaatttat tttgattttc catttgaatc ctcttctctt atctagcgtc cattctttta
    49261 gctattacat tcaacacgct cctcattatc ttataaacga ttgaaaattt caaccaaatt
    49321 tatttggaaa ggaggattcg cttgctaaaa tggtcaagta cgagaaaaat ccgtttttat
    49381 tgactcttta attctggtgt aagaagacca aacttcctct gatatatcaa tgtgaggaat
    49441 aaaatctttt cctttcggac catagccgtt ctcatcgtca tggagacgtt ccatttctcc
    49501 taatactagc gcatggaaat attccttagg ccattgatga ttcagttctt ttgctgtaaa
    49561 gatgatctct tcagaaaagt gaaggatttc cggcacaaat ggcacagcat tcgcgaaaat
    49621 agtttgttca ttttgccata ctttctcaaa agttgctcga gtcctgcgtt gcagtgtcgg
    49681 atcattcatc aataagattt tttctggtac agctcctaag gaatgaagaa tctccagtga
    49741 aaaagtggcg ttctctccag aatttgtaga cttcgattct atcagaaaag ccttatcggg
    49801 caaatcatag acttctttta gatactgacg acagatttca ctttcactca tgccatcttc
    49861 aaaatgaaat ccttgctttt caaaattctc ataaaggatt ctagtcgcat gccccacccc
    49921 tccgaccaaa aacaattgat ctacttggcc atttaaatac atttgtgctg ctttatcagc
    49981 taagatagga agacagtttc cagctaaaat aagtgtaggt gccgtcagtt cctcaaaatt
    50041 tccctcttca ggtgcggcta aaaaatttaa tagctgattc cagtctttta gattcctctg
    50101 ctcacttttt tccaaaggcc ttctatcctt tcatctgatt ttttgtgctt cattaaagtc
    50161 ttctcttttt gtctcaatcc atttgatttc tccatgacat cttccacaac gatatttgcg
    50221 tacatcgatt cttctcttgc ggcagatcgt ggcagaacaa tcttggcatc gataatactc
    50281 catctttatc tgtttcttcg tcaaggctgg tgcatagcgc aaccctcctg tttttttcaa
    50341 aagatacttg aaatccatat ctttatgctg ataccctctt ccagctagat gaagatgata
    50401 atggcaaagt tcatgtttga tgattcctgc aatcgtctct tcgtctgact cttcaaatag
    50461 cttcgcatta aaatctaaat gatgatcgtt taaatgatag cgcccgccag ttgtacgtaa
    50521 gcgtggattg aacattgctt ggtgcttgaa aggaagctgg aagcttttta atgatatctc
    50581 ttctactaac tgctgcagtt cttcttgtgt caatttttgt tactcctcat tgttactctt
    50641 cataagggga aagcatcgtc aaactgattc tgcctttttt cgtatcgact tgttctatcc
    50701 aaactgttac gatatctcct actgacacga catctgtagg atgcttgacg aattttttgc
    50761 tgagtttcga tatatggaca agtccgtctt gcttcacacc gatatcaaca aatgcgccga
    50821 agtcaatgac attacggacc gttccagtca attccatccc tggtttcaag tcttccatac
    50881 tcaatacatc tgtacgtaat aagggcgctg gcatctcatc acgcatatct cgtccaggtt
    50941 gtgtcagccc agctaaaata tcggctaacg tttcttctcc gactgatagg ctttcagcca
    51001 gtttctctac ggataatcgt gtcaattttt ctactgcttc ttctgtacca agctcttttt
    51061 ctgaaagttg gactgaagtt aatatttctt ttgcaataga ataactttcg ggatggatac
    51121 ctgtgttgtc caaaatgttt ttcccaccag ggactcgcaa gaatccgatt gcttgttcat
    51181 atgcttttgg tcctaagcgt ggcacttttt tcaattgagt tcgtgcggtg aattctccgt
    51241 tttcttcacg ataaatgaca atgttttggg cagttgtttt attcaatcct gatatatgct
    51301 gcaagagctg cgggcttgct gtattcacat ccacacctac ttggttaacc gctgtttcca
    51361 caacaaaatc caattgttca gccaaacgtt tttgagaaac gtcatgttgg tactggccta
    51421 ctccgacagc tttaggatcg attttgacta attcagctaa aggatcttgc aggcgacggg
    51481 caatagaaac agcacttctt tcttcaactt gtagatgcgg aaattctttt cgtgcaattt
    51541 cgctagcgga gtaaacagaa gcgcctgctt cgttgacgat agcgtaatag gttttatggt
    51601 cggcggattt caactgctca gccacaaaaa gttcagattc ccgactggct gtaccatttc
    51661 cgatagctac catatctact tgatatttat taattaattg gataaaagct ggtccagcag
    51721 cttctctttt tgcttgcgct gcaggtttat gaggataaat aacttcgatt gctaaaactt
    51781 tccctgtcgc atctacgact gctagtttac atccagtacg ataagccgga tcaaatccca
    51841 ggacaacttt accttttagt ggcggttgca acaaaagatt acggaggttt tcgccgaaaa
    51901 tagcgattgc ttgctcatct gccttttcag tcaattcatt gcgcagttct cgctcgattg
    51961 ctggctgaat aaagcgttta taactatcct gataggcttc tctgacgaat gaagaagaag
    52021 gagaggcagg atttttgact atttggcgat ccaagtaagc taaaattgct gcttcatcta
    52081 cttgaaagaa tactttcaga atatcttctt tttctccacg attagtagct aatattcgat
    52141 gagaaaccat tttatggacg ggttctgcaa agtcataata catttcatat acgccttttt
    52201 catctgcttg ctcatccttg acattgctga catacatacc tttgttatac gtataagacc
    52261 gaatccaagt ccgaaatttt gcattatcgc tgacttgttc agcaataatc tcatgagcac
    52321 cgtgcaacgc ttcttcggca gaagagactt ctttttcttt gtcaatatat ttttctgctt
    52381 cagattgcac ctctccatct gtaagctgca tcagccactt cgcgagtggt tcaagtcctt
    52441 tttctttagc aatcgttgct tttgtccgtc gtttttgttt gtatggacga tacaaatctt
    52501 ccacttgttg cattttcacg gcttgggtga tttctgcttc taactcggga gtcagtttcc
    52561 cttgttcatc gattagccga atcacttcag ttttacgttt ttctaagttt tcaagatagg
    52621 cataccgctc ttcgatttca cgaatctgga cttcatcaag acttccagtc atttcttttc
    52681 ggtaacgcgc gataaaagga acagtgtttc cttcatttaa caaattaagt acagtagtca
    52741 actgcttagg acgatagtct gaaagttctt tttgtacaag ctgaatgatc gtctggttca
    52801 aattttccgt cattaaaatg cctcttttct gctaaaaatt gcttctttat tctatcatac
    52861 tttttatcct gggttaaact actgaaagaa acaatattct ctataaaaat aaaaagagcc
    52921 gcaacaaagt cacagctcct tcattctttc cattagtcag attccatgcc aaggtccttc
    52981 gttgttctga tattcaatat cttgaagaat accttccttg tcaaaaacaa ccccatgcaa
    53041 tgaccctccg aatacagccc caccgtccat tccgatttta tgatcctgaa tccagaggtc
    53101 tgttgtctgc atatctccat ggagcattgg tgtaatcgta tgtccaaata caattgtctt
    53161 acctgtattg tttttccctt catgaaatgg ttcccttatc caaataaagt ctcttagact
    53221 tgtcttacgc cagtctttct ttgtcaaatc cacaccggca tggacaaaaa gataattttc
    53281 ccattcataa tacaaggggc gctggactaa aaatttaatc agttctgaat atctcgattg
    53341 gatcatcatc gagatttcag ttggggagta ttcctccgat gcccctgggt gaatcaggct
    53401 ttcaaacgtt tcttttccac catttctcat ataaggcgca taccagtcct ccggtgattg
    53461 cagaaattgc aaaaaatatt cttcatggtt tccccggagg taaatggctc ctgtttcttc
    53521 gactaattgc tttcctaata aaaaacattc ttttgtacga ggacctcgat cattgagatc
    53581 ccctaataaa acaagctgat ggatctttgg atcataatag tgaataagtt tttcaaaaag
    53641 ctcatgtttg ccatggacat ccccaatagc ataaatcaat gatttcatgt ggatcaccat
    53701 cctcaacgct agtatagcgc atattggaaa tggtcgacaa tcaaaactat tgttttctat
    53761 ttaattaaag ctgtaaaccg cagttctgct tcttctggag agattccatg gtcttgaaca
    53821 tagcgattgc gcggatgttt cgcacactct atgctacatg ctcctaaatg tttttcttca
    53881 ttttcaatgg aagcaagaat ctgacgattg cactcaggat tgctacaatt gatatatcgt
    53941 tcacatggtg ttccgtcaaa ccagtcgcga ccgacgattt gtttatctac ttggttgata
    54001 tcgacagcga tccgttcatc aaacacatac atcttgccgt cccataattc tccttggact
    54061 tcaggatcct taccataagt agcaataccg ccatgcagct gtccgacatc tttgaatcct
    54121 tcacggacaa gccaaccaga aaacttctcg caacgaatgc caccagtaca gtaggtcact
    54181 acccgttttt ccataaattc ttctttgtgc tcgcggatcc agtctggaag ctctcgaaaa
    54241 ctgcgtatat ctgggcgtac agcccctcga aaatgtccca agtcatattc ataatcattc
    54301 cgtgcatcaa tcaccactgt ctgttcatct aaaatagctt ctttaaattc ttgcggtgaa
    54361 agataggcac ctgtcacttg gtttggatca atgtcctctt ctagctttaa acttacaagc
    54421 tctggacggt atcttacaaa catcttctta aaggcatgtc cttcgcttgg atcgatcttg
    54481 aacatcgtat cagcaaatcg aggatcagct ttcattgctt tcatataggc agtcgtgttt
    54541 tcaacggttc cagacacagt accatttatt ccttcttcag ctactagaat acgaccttta
    54601 agatccaaag atttgcaaaa ggctaaatga tttttggcaa attcctctgg ttcttcgatt
    54661 tttgtataat tgtaataaag cagtacttga taatccatgt atttcttcct tccttctttt
    54721 cgttcctaaa cagtataaag aaggcgttcg catttgacga tttatttgct tgtgtataat
    54781 tacgcatgga aagttttttg tttacataat attattattt gaattaaaaa gacttcgctt
    54841 tcctgtttcg cacagaaaaa caaagtcttt atttctatta tttacctgta accgcaaagt
    54901 agttgccatc gggatcagca aagttaaaga catattcttc tccaagctgt accatatcac
    54961 ctaatgtgac tgattgcgtc tgcatttttt tatatagtcc aaagatatct ttactgtaaa
    55021 acatcaaaga tggggcacta gtagctgtgc caggtgaatg ggactcaaca aatgctcgat
    55081 catataacag aaatgaggtt tgactgtcct caactcctac ttcaattata agcgtcccat
    55141 ccatttcttg acgatctttt tccgcaaagc caactgctct ccaaaacgtt gcattttttt
    55201 ctacatcgtc cacataaagc atgattttta tttcattcgt aaacatct
//
LOCUS       DAYYKN010000004        50165 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000004 DAYYKN010000000
VERSION     DAYYKN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.004"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..1168)
                     /locus_tag="ACHBB7_000174"
     CDS             complement(join(42..940,940..1168))
                     /locus_tag="ACHBB7_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9208160.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1572..2354)
                     /locus_tag="ACHBB7_000175"
     CDS             complement(1572..2354)
                     /locus_tag="ACHBB7_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208161.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(2419..3255)
                     /locus_tag="ACHBB7_000176"
     CDS             complement(2419..3255)
                     /locus_tag="ACHBB7_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9208162.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(3273..4682)
                     /locus_tag="ACHBB7_000177"
     CDS             complement(3273..4682)
                     /locus_tag="ACHBB7_000177"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9208163.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(4696..6519)
                     /locus_tag="ACHBB7_000178"
     CDS             complement(4696..6519)
                     /locus_tag="ACHBB7_000178"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9208164.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(7362..7688)
                     /locus_tag="ACHBB7_000179"
     CDS             complement(7362..7688)
                     /locus_tag="ACHBB7_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208165.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(7734..8096)
                     /locus_tag="ACHBB7_000180"
     CDS             complement(7734..8096)
                     /locus_tag="ACHBB7_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM9208166.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(8133..8347)
                     /locus_tag="ACHBB7_000181"
                     /pseudo
     CDS             complement(8133..8347)
                     /locus_tag="ACHBB7_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            8475..8810
                     /locus_tag="ACHBB7_000182"
     CDS             8475..8810
                     /locus_tag="ACHBB7_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9208167.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(8886..10883)
                     /locus_tag="ACHBB7_000183"
     CDS             complement(8886..10883)
                     /locus_tag="ACHBB7_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208168.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(10981..11256)
                     /locus_tag="ACHBB7_000184"
     CDS             complement(10981..11256)
                     /locus_tag="ACHBB7_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9208169.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            11488..12222
                     /locus_tag="ACHBB7_000185"
     CDS             11488..12222
                     /locus_tag="ACHBB7_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9208170.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12261..13403
                     /locus_tag="ACHBB7_000186"
     CDS             12261..13403
                     /locus_tag="ACHBB7_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9208171.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13498..14889)
                     /gene="sufB"
                     /locus_tag="ACHBB7_000187"
     CDS             complement(13498..14889)
                     /gene="sufB"
                     /locus_tag="ACHBB7_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM9208172.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14894..15364)
                     /gene="sufU"
                     /locus_tag="ACHBB7_000188"
     CDS             complement(14894..15364)
                     /gene="sufU"
                     /locus_tag="ACHBB7_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM9208173.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(15351..16586)
                     /locus_tag="ACHBB7_000189"
     CDS             complement(15351..16586)
                     /locus_tag="ACHBB7_000189"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM9208174.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(16583..17869)
                     /gene="sufD"
                     /locus_tag="ACHBB7_000190"
     CDS             complement(16583..17869)
                     /gene="sufD"
                     /locus_tag="ACHBB7_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM9208175.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(17882..18655)
                     /gene="sufC"
                     /locus_tag="ACHBB7_000191"
     CDS             complement(17882..18655)
                     /gene="sufC"
                     /locus_tag="ACHBB7_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM9208176.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(18852..19700)
                     /locus_tag="ACHBB7_000192"
     CDS             complement(18852..19700)
                     /locus_tag="ACHBB7_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9208177.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(19724..20410)
                     /locus_tag="ACHBB7_000193"
     CDS             complement(19724..20410)
                     /locus_tag="ACHBB7_000193"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM9208178.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(20403..21437)
                     /locus_tag="ACHBB7_000194"
     CDS             complement(20403..21437)
                     /locus_tag="ACHBB7_000194"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM9208179.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(21500..21756)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(21832..22170)
                     /locus_tag="ACHBB7_000195"
     CDS             complement(21832..22170)
                     /locus_tag="ACHBB7_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM9208180.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(22172..22528)
                     /locus_tag="ACHBB7_000196"
     CDS             complement(22172..22528)
                     /locus_tag="ACHBB7_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM9208181.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(22534..23721)
                     /locus_tag="ACHBB7_000197"
     CDS             complement(22534..23721)
                     /locus_tag="ACHBB7_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9208182.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(23912..24757)
                     /locus_tag="ACHBB7_000198"
     CDS             complement(23912..24757)
                     /locus_tag="ACHBB7_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9208183.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            24991..25956
                     /locus_tag="ACHBB7_000199"
     CDS             24991..25956
                     /locus_tag="ACHBB7_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM9208184.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            26333..27376
                     /locus_tag="ACHBB7_000200"
     CDS             26333..27376
                     /locus_tag="ACHBB7_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM9208185.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(27438..27902)
                     /locus_tag="ACHBB7_000201"
     CDS             complement(27438..27902)
                     /locus_tag="ACHBB7_000201"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM9208186.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            28148..29095
                     /locus_tag="ACHBB7_000202"
     CDS             28148..29095
                     /locus_tag="ACHBB7_000202"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9208187.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(29306..31378)
                     /locus_tag="ACHBB7_000203"
     CDS             complement(29306..31378)
                     /locus_tag="ACHBB7_000203"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9208188.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(31311..31688)
                     /locus_tag="ACHBB7_000204"
     CDS             complement(31311..31688)
                     /locus_tag="ACHBB7_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM9208189.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(31817..32377)
                     /locus_tag="ACHBB7_000205"
     CDS             complement(31817..32377)
                     /locus_tag="ACHBB7_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9208190.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            32527..33819
                     /locus_tag="ACHBB7_000206"
     CDS             32527..33819
                     /locus_tag="ACHBB7_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM9208191.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(33879..34997)
                     /locus_tag="ACHBB7_000207"
     CDS             complement(33879..34997)
                     /locus_tag="ACHBB7_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM9208192.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(35011..36432)
                     /gene="arcD"
                     /locus_tag="ACHBB7_000208"
     CDS             complement(35011..36432)
                     /gene="arcD"
                     /locus_tag="ACHBB7_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM9208193.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(36678..37799)
                     /gene="mnmA"
                     /locus_tag="ACHBB7_000209"
     CDS             complement(36678..37799)
                     /gene="mnmA"
                     /locus_tag="ACHBB7_000209"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM9208194.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            38203..38802
                     /locus_tag="ACHBB7_000210"
     CDS             38203..38802
                     /locus_tag="ACHBB7_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9208195.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(38940..40226)
                     /gene="efmA"
                     /locus_tag="ACHBB7_000211"
     CDS             complement(38940..40226)
                     /gene="efmA"
                     /locus_tag="ACHBB7_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM9208196.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(40497..40841)
                     /locus_tag="ACHBB7_000212"
     CDS             complement(40497..40841)
                     /locus_tag="ACHBB7_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM9208197.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(40978..42123)
                     /locus_tag="ACHBB7_000213"
     CDS             complement(40978..42123)
                     /locus_tag="ACHBB7_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9208198.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(42303..43277)
                     /locus_tag="ACHBB7_000214"
     CDS             complement(42303..43277)
                     /locus_tag="ACHBB7_000214"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9208199.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(43357..44232)
                     /locus_tag="ACHBB7_000215"
     CDS             complement(43357..44232)
                     /locus_tag="ACHBB7_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM9208200.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            44362..46269
                     /locus_tag="ACHBB7_000216"
     CDS             44362..46269
                     /locus_tag="ACHBB7_000216"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM9208201.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(46810..50088)
                     /locus_tag="ACHBB7_000217"
     CDS             complement(46810..50088)
                     /locus_tag="ACHBB7_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM9208202.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT        14381 a        10513 c         8839 g        16432 t
ORIGIN      
        1 ggatttgagt caatatcttg gacacatttt agtagagacg gttaagccat tttttctttt
       61 gctaattctt caaattcctg tggggttaga tagtgaatcg agctatggat tcgatttcgg
      121 ttataaaatc cttcaatgta gctaaatagc gctcgatttg cttcttcaaa atctgagtag
      181 ctagtcgtgt agacttcttc ttttttcaat gaggcatgga aagattcgat tccagcatta
      241 tcataaggtg ttccttttct actataagaa tgccttattt tgttggtttt aagccattgt
      301 tccacttctc ttgctgtgta ttggctgccc aagtcagtgt gtagaatcat tccttctgga
      361 atgtgataat gtatttttgc tttattcaat gtttgaatga cacaatccac agtcattcgt
      421 tttgaaaatg tataactaat aatttttttc gtgtgtaaat ccatgattga agataagtaa
      481 caccaaccat ttttcttagt aggaatgtac gtaatgtcag ctgcccattt ctcacagatc
      541 gtttcagtag aaaagtcctg atttaagaga ttctcttttg aaatgatcgg tttattagac
      601 ctttgaggtc tatatttttt aactacaatc gaccttaaat ttaattgttt cattagcttt
      661 tggacatgtt taagagatac tgaaatccct tcttttaata aaacttgatg gatctttgtg
      721 gcaccatagc gttgtttatt cgtaaaaaaa attgttgaaa tcttcttttt caacttatta
      781 tttctgattt ctgtagctgt caacggttta ttcttataaa aataatagac acttctaggg
      841 atttctaata accgacataa caaagaaaca gtgtaatcct tacaccattt ttcaataaat
      901 tgaacaagcg aatttaggtt ttccgagaga atatggctgc ggcttttttt aagatttcaa
      961 gttcctcatt taaacgagca ttttcttttc tcagttcagc tacttctttt ccagtcagtc
     1021 ctgtggactg atcaggtaaa tataaattct tccatttgta aatcgttgct tcagataagc
     1081 catattcttt cgctaaccct cgaacagaac gtccagtttg attcagttca acaatcattt
     1141 gtttaaaatt ttcttcatat cgggtcataa aaattcatcc tctcgtagat aagtttaatt
     1201 tattcttgtc tctacgaaaa gctgtctaag atttgatact aacaccaagt tatctttaga
     1261 accatttcag atacatatga aacaattaca tatgattttt tcataaaaaa acagtactgt
     1321 ctagtagtat aaaatataca aatttcttaa aaattttttc actaaaatca ctacctgaaa
     1381 cagtctaaaa cacttatata tcaacgttta atgaaactat ctccgtttca tttcagatac
     1441 aaataaatat agtcgggctc caaatccgaa aaaaaatacc cccagtagct tatcctaagc
     1501 tactggagat gtataagtac ggtcatataa caacggggta gggtaaataa atgaaaaaga
     1561 tggaagaatc attattcaat aactttttta aatacttcgt tgtaatcagt tacctgtccg
     1621 ttcttaaata ccacagaata atcttttttc ccattttcaa gtaaccgaag tggaatgtct
     1681 ttaccaactt gctctgtcac tgcattactg atacctgtta gattagctaa taaatctgat
     1741 aaagattgag catctgtaga aatctctgta tcagtattta tcgtgattcc tttatcttca
     1801 tttacaagct tccaagtagg tggtatagct gtgtatgttt cgatgaatga atcaattctt
     1861 tcatctatcg tcttcccagt atctacttct gtctcattac tcttagatga atcgattctt
     1921 tcatccatcg tctgctccgt aactgcttct gtctcattat tctcaccgga aactctcttc
     1981 acacccaaac ttatatattg tccatcttta gtgtgcatag ttaaaattcc cacgctttta
     2041 ctaataaatt cactataacc agttctttca atagagactt caatgtattt cacactatca
     2101 tactcaaaag aaaaatagac atgtaaaagc attttgttgt ttacatctac ataataatcc
     2161 tttgactcta cttgagacct tcctatacgg aatacagctt tactgtctga tatctctata
     2221 ccagcatcct tattattaat ctgccaatct ccaagtagct cttctggcaa gctagtagtt
     2281 tcttgtgaac actttaccac ttcactggta gaggtcgaaa cattcacctc cgcactgtta
     2341 gaagaatttt ccaaggaatc aggagcctta gcacaaccaa caagtcttta tatattgtgc
     2401 agccgatatt ttcttcattc agtacaccct tgaaatatgc agtgttagat aaagcagttc
     2461 gtcctgattt gctgaggcac ccatctcttc ttgaacgtac tccttgattt tcaaggccgc
     2521 ccgatactct ttcggaaaac gctgcttcac tgaagccagc aaaaaatcat ccttctccgg
     2581 caactcttcc ttcttaatca ttcgggcaat aaaaaactgt aaatgcgtca caaatcttaa
     2641 gtaaagcatc gaatctttat caaccttttt ttcgggaaac agatatccta gcatagccaa
     2701 gatatccttt agtgctctta ccgagagcat ggtttcttcc atatttgctt gttcactttg
     2761 ggcatttaca atgtgaaacg caatgtttcc agcctcctct tctggcaact gaacattcat
     2821 ttttttgttg atcaattgga tagcatagca gccgatctca aattcttttg ggtaatactt
     2881 ttgaatttca atcagcaggc gattttgaaa aacaatgcct ttttgactgc gttcaatggc
     2941 atgcttcaag tgatcaacca aggtaaaaaa tagactttta ttcagcttaa agtcaaactt
     3001 ggcgttggca taatcgataa ttcgcttgac gcaaataatt agatcatccg gagcagcctc
     3061 aaacagttta atgtaatcct tttttagagc atcagaaagt gaatctaatt taaatacttt
     3121 ttggattttg tcagccggaa ccaaatcgcc tttctttagt tgaaatccaa tccctttccc
     3181 catcaaaatt acttctttgc cttgcttatc taagcaaaga attaaactgt tgtttaacgt
     3241 tttaattact ctcattagat tcaccgttct ctctaagcta agccatttct tgcgattact
     3301 ttttgatacc aaaagaagct ttcttttttt attcgtttta attctttaag attctcatcg
     3361 gttcggtcaa catatataaa accataacgc ttattgaagc cttgatgggt actaacaaca
     3421 tcaatcgctg accaaggtaa ataaccaaaa acatctacac catctgttaa agctgcttga
     3481 atagccagaa tatgtttggc tagataatca attcgatcct ggtcattaat tcgtctatct
     3541 tcctcaacta tgtcgggtgc cccatagcca ttttcagtaa ttaaaatagg taaatcatag
     3601 cgatcgtaca atttcctcaa tgttaatcgt agtccagtag ggtcaactac ccagccatat
     3661 ggcgttttat ctacataagg attttcagct gcccggtaga caccttcttc tcccaacatg
     3721 atctgttgat cccccgctct agcggaaaca tctgatccgt catttttgct ggcagcgatg
     3781 gtggcagttg aataataatt aatcgcaata aaatcgggtt taccggagga taaaattgcc
     3841 tgatcctctg cctcaatttt aggatataat ccacgatcat tcaaatagtt ttcaaacaaa
     3901 taattgtatt ttccccgaac tgctacatct aagaaagacc agcctctcaa tgtttcccaa
     3961 ttatgggctg caattgcatc ttctggactg gaggtagctt gatacatagc cgttaaattg
     4021 attgctggac cgatttttcc tttaagattc aactgatgat acaagtgaat tactctagct
     4081 tgagctaata aaacatggtg attaatttga aagagttctt tttttgaagg cagctctcca
     4141 cttggtggca aaccaatcgc tccaggatga aggatcatcg tgttttgctc attaattgtc
     4201 agccaatagt tcaccttgtc accatactca gtaagcaata cctgtacata gtccacaaaa
     4261 taatcgatag ttgctcgatt taaccaaccg ccctgctcct ctagaaaata aggtaaatcg
     4321 aaatggtaca tggtgacaat cggttcgatt ccctgattca ggcattcatc aattacttga
     4381 tgataaaaag aaattccctt ttgattggtt ttattatttt caatatccat aattcgcgtc
     4441 caagcgatgg aaaagcgaaa agcttttaag cctaattctt taaacagctg tatatcttct
     4501 ttatagtgat ggtaaaaatc cgaggcaact tcaaaattag ctaccccttc tgtatgctga
     4561 tacttgtcaa taatagacgg ttgtttccca tcctcctgat aggcaccctc tgcctggtag
     4621 gcactaatgg aggcacccca taaaaacttc tctggaaatg ctgaaagctg tttgtattgc
     4681 acaaaaatcc tctccttaac taatttgcat caatggttcg tgttccttta agcttccgct
     4741 tgcttcacta attcctgcgt aattttcact gttggtcact accacaatca ctgtatcatc
     4801 gtagccagcg tttctaatgg cagcgaaatc ggcgttgatt aataattgtc ctgctgacac
     4861 ctgatctcct gcttttacaa atacttcaaa gggttcccct tttaaattca cggtgtcgat
     4921 ccccacatgg atcaataatt caataccttc tttagaacgt agaccaatcg catgtttaga
     4981 ttctaaaata gtaatgactt ctccctcaac aggtgaataa atgctgtttt cttctggatg
     5041 aatggctacg ccctttccca atagtccctt agaaaagaca gcatcgttca cttcagaaat
     5101 aggacacacg gttcctttta ccggattttc aacaatgact gcttcggcta ttgccgtttg
     5161 ctctgattca tcagaagtat cgtcaaatcc aattagataa gcaatgataa aggttgcaac
     5221 aaaggcgatt acacacgtaa taattgcgtg aacaatattc attggatttt ctccaataaa
     5281 ggctggtaag gctgctaagc caggggaaac aaaggcatat ctgactaaac cgctcaagcc
     5341 agcatacaaa ccagcaattg caccaccaat catggaagca actaatggtt ttttcagctt
     5401 caaggttact ccatagagtg aaggctcagt aattcccatt aaagcagtga tcccagtgga
     5461 cacagctagc tgcttcaact ctttgttttt agttttcgcg gctacagcaa aggtagcacc
     5521 tgattgagca atattagaag ccagcattcc tggcccattg atcatttcag aacccgtact
     5581 gctcaattgc atagtagcga ttggcgtcat tgcccaagct gtacccgtaa ccaccaaaaa
     5641 tggctgcaaa ccacccatca aaaatggaat taaccaagct gctttgccgt taatccattc
     5701 agcccccatt gctataagat cgttcagcaa cgttccaaac ggtccaatga caaccaacgc
     5761 taaaggcccc ataattaaaa ataccagcat cggcttagta aagaatttaa taaacgaagg
     5821 cgaaattcga tccgcaaagg gctctacata ggacatgatc caaacagtaa agataatcgg
     5881 taaaactgat cccgcataat ctgcagctaa taccggaatt cctagaaaag aaacggcatc
     5941 gccagtagaa aaaagctgtg aaatatttgg atgaagcaaa atgccagcaa cagtaatcgc
     6001 caaaattggg ttgacatcaa actttatggc tgccccatag gctaatagta ctggcataaa
     6061 ataaaaggct gcatcagcaa tagtattcaa aatgatgtaa gtttgactac tgtctgatag
     6121 tacaccagtc aaagtcaaga cggccaaaat ggcttttacc atcccggcac ctgttatagc
     6181 tggaatcatc ggtgtaaagg tggtagaaat gacactaatc aatcttgaaa taatactttc
     6241 ttgattgtct gaagaggggc cctttttaat tgaagttccc acttcctttt gaatttcctt
     6301 gaaggctgtt tggacttcat tgccaatcac cacttggtat tgtccaccgc tatccaccac
     6361 actaattaca ccaagtgttt tttctaaggc tttcgtatcg gctttccttt tatctttgag
     6421 ttcaaaacgc agtctggtgg cacaatgggt taagctgacc acattttctt ttcccccaac
     6481 ctgttgcaat acatttcttc ctacttctct gtaatccatt attctctctc cttaaagcag
     6541 taaaaaagac caagtcaaac cacgccccaa tcaacattga agtgttagtt tgtcttggtc
     6601 ttgcctgcat aatcagtaac atgccaataa cgttattttg ctgtaatcca ataatagcac
     6661 attatgatag cgcaatcaac agggaattta attttttatc ttttcgtttg accgtatagg
     6721 gtaatataac agttagatca catcaactag ggtatatctg cgtatactta ctgctaaaat
     6781 aagtatatct aatattaact ataccagtaa aaccattcct acataaccag cgggaaattc
     6841 ccaaaatcgt gaaagcatca aaaatgcata taacggatca cacaacttca acaattctgc
     6901 tcttgggaaa aattgatgaa tttggtgagt aaaggatgga ataaacagca ttccaacaat
     6961 ataggcaaca atagtgatac ttgttttttt ggtaaaaatc agtaagcaat agccaatttc
     7021 aatcattccg agatataaag gtatcgatag aatgatttgg gctagtattc ttaaaaatta
     7081 actagcaaat gtttaaactg atttatttct aatctacatg tagtaagtat ttcaattcca
     7141 ccaattatca ataaaaatac caaacatatc cataataatt ttttcttttt catttctttt
     7201 atattgttat aacagtgaat aacataaaaa ttcccgacaa tcattgtaat atttttttgt
     7261 cacctgtatt agtatttaat ttataggaaa ctataaaaac tccctagtga atttacacct
     7321 ttgggagttc ctgtttggac ggtatgcata atacatttaa atcaatttaa cattaacacc
     7381 tcagcatatg gtatctccac atatatacgg tctttctttg gatagtactc aatatttatg
     7441 ttgatagcac ttccattgtc taatatagct ttagcccggt atgtgttatc tccattttca
     7501 tgagtaatcg tcacatcatc acatgatcga tttaagttgt actgttcttg taaaatctgt
     7561 gttaccaatg gtttcgatga cttctctaat ccagatgtac ctgtcatttt tgaaaaaatg
     7621 atacctataa tagccaagac aacgataact ataatgataa acttcttgtt catatttact
     7681 ttattcaact ttttatctcc tatctccaaa ataatttgct cttatccctc ttatcagtac
     7741 attgacatcc ctaaaagtac accaaagatt gctccagcta cccctgctat catcaaaatc
     7801 gtacccattt gttcatcttt atctcgatat aagtagccca taataacagc aacagcacca
     7861 aatacaacag gaataaatgc taacgctact accatagaaa tccaaccaat aatcaaatac
     7921 agcttccctg tctcttgtcc atgtacgatt tgttccttca tttcattaat tgtctgctca
     7981 gtacttttct ttactgcgtc tgctccagtt gtctcatctg tttcttgtag tgctccgcaa
     8041 aactggcaat acttactttc atctgttatt tctttgccac attttctaca atacatgtct
     8101 tctcctccta aaaacattca attgtttatt agttaactgt ttactttatt aaatacatca
     8161 gaaaacagct cattcattgg tacacttaat atctcagaaa gcatcttcat ttgatagggt
     8221 ttaggaatcg ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt
     8281 ttgctaactc cgtttgagaa agtccttttt cttttctgta tcggctgaga ttacttactt
     8341 ttttcatgat tctcccctcc tgttttatta tatacaacaa tatgatgtat ttcaactcat
     8401 aaaatataaa aaaacttcaa ataccattac aaatacaaca acttgttgta ctatactgag
     8461 atgggggtgt attaatgaaa cagaaacaat atgcaaaggt cattagagaa aagcgaaaag
     8521 ctctcgggtg gacacaaaaa gaattagctg aaaaaatctt ctctacacaa caggcagtag
     8581 caagatggga aaattctgta acagagccta acctcgatag cttaaccgct ctttctagag
     8641 ccctaggaac ccctgtaagc cattttctag acaatgttgt cgtagattat gaggaagagt
     8701 ttttggctct ctatcgttct cttagcactg aagatgcagt tcgaacaatt gactatatga
     8761 agctattaaa aaggcaagaa aatgaacgca atcagctatt gaaagattga aaaataaata
     8821 acccaccgaa ctttaacggt gggtgtattg agatgaaata agattaagag ctatggataa
     8881 cctgtctagt ttaatacaat tgggtctttt ctcttcctta ctctccgtcc atcatctctt
     8941 gttacacttg taaattgata aagaggaact aattcaactc ctgataatat caattctttt
     9001 attacttcat ctatccctgc tccttttgct aggtagacta aaaaactact acttgctttc
     9061 acattgtata gtctcttaat atccttagaa gctcgttcaa gagtgaatcc ttgtttaaca
     9121 aagtagcctc tgttttcttt taaagcattt tccacgctgt tattcccaac attacgtaga
     9181 cgttcacaaa cagtgttagt aagttctttc tctgtcatta gatatgttcc ttgccacgat
     9241 aagccctgta aaacttcatg tgcttcatct aaaatcaatt gtttttcctt gctaagttct
     9301 gatcgggaat tttgaggaaa tactctttta gcctcttttt cgttaaaaca tatcttaacc
     9361 cattcataag aaaaatattg atatgtaaaa tgaaattttt ccagaagtgg aaggatatct
     9421 tcattcaatt tttgaatcac agaagttgca ttaaaaacgt ctaatcgata gacattgctt
     9481 ctcttatgat taaagtttcc taacgctttc tcttccttta acctatttag gtattcgagc
     9541 atcttcaaag gaacatcttc atcatctaat ttttttatca atcctataaa agtcataagt
     9601 gatagtaact tattacattt atctatgtca taagttttac catagtaaaa actcattttt
     9661 ttagctaatt gttcccccga caaaatacaa gtcacacttt ccttactatc atcaaaaaaa
     9721 ttaacatcag aaagcaagac atcatagaca cgtcgcattt cctctagata attccttatt
     9781 gtcctgaaaa gtttgtcttt cattgattca agagtgtcaa aaagtacttt tgattcctct
     9841 ttcaattttc gaaaatgtgt aggatacaag tctaggttct ctaatagcca attaaatgtt
     9901 tctacccgac tgtctttctt taaaattagc atcacaactc caataaaatt ttcactgata
     9961 tcacaattac tggaatgaca ataaaaaatg gaatgcccag ttttactggt aaaaatacta
    10021 gcagagggat tatgatcgtt gtgaaacaga catcttaagt ttttacttga cttaagtaat
    10081 ttattcatat caatggttaa caatctttca taaatctcat taaaagacat gtcggaggaa
    10141 tcaaacaggc agtctttaaa ccactgtttc atttcttctt tgtcgtcatt ttttatcaac
    10201 ttaacaaaat cggtttcatt gactagttca tactctctcc ctgaatcact ttgacaattt
    10261 ttagaacatg tcatggtaaa atcaatcgga agctcagtaa tatctaacat gttgtcttca
    10321 ttaattatta tcactgcatc tgacttatat cctccaaaaa acattctatt cgaagcattt
    10381 acattgtcat cacacccggg gatgttcttt ttcaagtatt cgtaaacagc aaccatttta
    10441 ccatagtcag taattgcttc ttttaataga aagacaatct tgtatttttc tagattttca
    10501 gagtaccgaa tacttttttg tatcgcaaac gcagaattat gaactaaatt gatagataaa
    10561 accttttcta tgcttaagta tctactatca tctttactca aatctactac cttccccttc
    10621 tcatctttaa cagtgttatc gacatccaga ataattactc tggtttccac aaaactctcc
    10681 ttgtcattgc ctttattctt tgtcttgctt ggaataaatg attgtgatga atttagtatc
    10741 tctacaaact catctcttgt aacttctcta tagttatcta aacacctatc tcttacatca
    10801 gttttttctt tttttgtaac tggcttctct aataattttt ctttatctac ataaattgga
    10861 atattaatca ttgatgtttc catttgtgtt tcctccttat aaaattctgt taaagactaa
    10921 aacatacaga taataaaaac aaactcttta ttactatatg ttttagtctt atttgtttac
    10981 tcaaccttta taccgttcca tgaaactatc aatatcagat tttttaaatc taatgaccgt
    11041 atcaattttt ataacaggta acccgtattg agaaacatat tttctaatcg ttcctactga
    11101 tacacctaga tattcacttg cctgctgata gtccatatac tctagagtga ctccagaaat
    11161 aatgtcttct agcataggct ttagcactgt aattatctct ttcacttgtt gttcaacttg
    11221 tatagaaaca atgctatcaa tgatttgtag agacatattt ccccctcctt tcgtgattgc
    11281 ttgaacaaat tttctaagaa ttatcttcgt attttttgct caaaataaaa gtattttgtt
    11341 attaaccgat taatcaaaca aaaaatgatt accagttcat cgacatacac ataatacctc
    11401 gataaaaaag aggtgtcaat atctttgtaa aatttattta caacacccct ttttcgaggt
    11461 atactaattt taggaggtgt ggtcaatatg ataaaacgtc caccaataaa ttatttagaa
    11521 cgaaaaaaga tactcggaac aaaaataaaa gctataagaa agagtaaaaa attaacacaa
    11581 ccagcatttg gtcttatgat taacaacggt caattaattg acaaaaaaac tatctacgaa
    11641 tgggagaagg gaacctattt accaatacca gagcgattat cccgaattgc tgatttggga
    11701 aatatgagta tagaggagtt agtttgtggc aatgtagaag aatatatttt gggaattatt
    11761 ctttatagag atagcattgt actagatggt ataacatttc cagataaaaa cctgttccag
    11821 catcttcgtc aacagttccc accagtacac tctaatttag atacatggct ggatagatat
    11881 tccaaattag aaccagagat gcaagaattt attgctaaca agacttgtaa taaagttaaa
    11941 aatgaaaaaa ttagtctttt caatatctta aaaatagaag aattattcat caatgctatt
    12001 gtagaagaat ttgacaataa cattcttttc ctcactagca gtatagaaga actactagaa
    12061 agaatggtag atgaatggct acctattcaa ttgaaagaca tgtcttatcc cgaagaagct
    12121 gtgagagaaa ttactgataa tataaacaag ctagaacaaa cgatttcatc aattgggaaa
    12181 aaatacacta agaaaaaaat gaagggaggt gataccatct agtggtaagt actccttgta
    12241 aactaagagg agatgatttc atggcaaaaa tatcaaagta caaaaagaaa aacggtcaag
    12301 tggcgtggat gtataaagga cacattgcca ccgatccacg aactggtgaa aaaataaata
    12361 ctacgagaag aggttttagt agcaaacaag aagctcaaaa agattatgac gagtacagac
    12421 atcaaatatt atacggtgtt aaaaagaaag cacctgatat gacatttgaa gacctgtata
    12481 atgaatggat aactcatcag cgtaccagtg ttaaagcttc aacaatcgca atctcagtaa
    12541 gatacgcaaa taatcaaata cttccagcat ttggtaagct taaactatcg aatatctctg
    12601 tcccatattg tcaaaaagtg gtagatgaat ggcacagtaa atatgaaagt tacgattata
    12661 tgagaaaaca gactgcacaa attttacgct acggggttgc aatgcaatat attgacaata
    12721 atcctatgga aaaaacactt cttccacgaa aaaaagagta tgagaaaaat cgtaaatttt
    12781 actcaaaaga ggagcttaat aatttactgg atgcttttaa agattttggt aacatgaaac
    12841 aatacgcctt cttcagatta ttggcataca caggtatgag aaagagtgaa gtactcgctt
    12901 tacagtggaa agacatagac acgtttaata aagaactaca tgtcaataaa actcttgctg
    12961 tagatgaatt tggtaaagtt ataattcaat ctccgaaaac aagagcttct agacgagtta
    13021 tctctttaga tactgagact ctttctatcc tcaacaactg gaaattacag caaaaagaag
    13081 agtatttaaa acttggatac aatactagct ctaaagagca acatgttttt acaacagtta
    13141 aaaacactct atacattcca aacactgtta atgattggtt aagatatatt ttgaaaaaat
    13201 ataacttgcc tcgtattaca cctcatgggt ttagacatac acatgctagt ttattattag
    13261 aagcaggaga atctgtcaag gtagttcaac aacgattagg gcatgaaaat agtaaagtga
    13321 cattagacat ctatgcacat attacaaata atgcacctaa gaaaacaggt caagactttg
    13381 ctgatatgat ggctcatcag taaaagagct ggtcaaaatc tggtcatttc tagtatacag
    13441 gcaaaacaaa aaagctagaa cccttatata tcaagggttc tagctcctat aacaggttta
    13501 cccaacactg ccttccatct cataactgat caatcgattg agttcgactg catattccat
    13561 tggaagttct ttggtgaatg gttcaacaaa gcccatgacg atcatttcag ttgcttcaga
    13621 ttcggtcagg cctcggctca tcagatagta gagttgttct tctgagattt ttgagacttt
    13681 ggcttcgtgt tctaaagaga cttggctgtt gtggatctca ttgaatggga tcgtatcgga
    13741 tttcgaccat tcgtccatga tgatcgtgtc acattcgata tgagagatcg aacccgcact
    13801 gtttttggca aaggttactt gtcctcgata gtttacttct ccgccgtctt ttgaaattga
    13861 tttagagacg atagagctag atgtatttgg tgcattgtgg atcatttttg cacccgtatc
    13921 ttggatttgg tttgctcctg caaaggcgat ggagagcatg gtgcctcgtg caccttttcc
    13981 atctaagtaa acacttgggt atttcatcgt tgtttttgca cctaggtttc cgtcgatcca
    14041 ttcaacggtt gcgccttcgt atgcttttgc tcgtttggtt actaagttgt acacattgtc
    14101 tgaccagttt tggatcgttg tgtaacggca atacgcatct tttcttgtaa agatttctac
    14161 gatggcggca tgcaggctgt tacttgaata agtaggtgcc gtacaacctt cgacatagtg
    14221 gatgcttgct ccttcgtcaa caatgatcag cgtacgttcg aattgccccg tgttttccgc
    14281 attgatccgg aagtacgttt gtaatggaac atcgactttg acgccttttg gcacgtaaat
    14341 aaaggtccca cctgaccata cggctgaatt cagcgctgct aatttgttgt cggttggcgg
    14401 tacaagtttt gaaaagtact ctttgaataa ttcagggtat tctttcaaag cagagtcagt
    14461 gtcagtaaag acgattccta acttttcgaa ttcatctttc atattatggt acacgacttc
    14521 tgattcatat tgtgcggaag caccggctag ataagctcgt tcagcttctg gtataccaat
    14581 cttttcaaat gtatctttga ttttttctgg tacgtcttcc cagtcacgag cgggtttgtc
    14641 acttggtttt tggtaatatt tgatggcatc gaaatcgatg tctgaaaggt cggggcccca
    14701 tgtttgcatt ggcattttgt taaatgtttc tagagatttc agacggaatt caagcatcca
    14761 ttctggttcg cctttgattc ttgacatctc acggatcact tcttctgtca agccttctcc
    14821 tgtactgaaa accggttcga catcatcgtg aaacccgaat ttatattctt ctaattctgg
    14881 tacgcccatg ttctcactcc tcttccttgt ggtaatgatg gaaagctgcg gtaccatcgt
    14941 tttccattgc ttttcctagt gctttccagg caagtgtcgc acatttgatc cgagcgggaa
    15001 atttagctac accaccaagc atagcagcat cgcctaattg gtcgggatct ttgacttctt
    15061 ttccttggac aagctgtaga aagtctacag ttaaatgttc tgcttcttct attgattttc
    15121 cgatgacggc gtctgtcatc atcgaggcac ttgccgtact gatggagcag ccactgccgt
    15181 caaaggcgat atttttgatg attccgtctt ttacatcgac ttgcaattcg atcacatctc
    15241 cacatgtggg gttgttcaat tcgatcttgc ttgtcgagtc agtcaagctt ccacgatgat
    15301 gcggatggga agaatgatcc aagatcactt gacggtataa attgtctaat ttagatagtc
    15361 ccatgttgga aaaactcctt tgttgccatg attgcttcaa ctaaacggtc ggcgtcttgc
    15421 gttgtgttgt agaaataaaa gctcgcgcga gcagtcgctg atacttcaag gtacttcaat
    15481 agcggttgcg cacaatgatg acctgctctg acggctaccc cttccatgtc taaagctgtc
    15541 gcgacatcat ggggatgtaa cccatccaaa ttgaatgcaa cgactcctgt ccgaaaattc
    15601 gggtcttgtg gaccataaac agtcaatcca tcaattgctt gtaattttgg caacacataa
    15661 gcggctagtt cttcttcata tcgatggata ttgtccatac cgagtttttg caaataatcg
    15721 attgcatggc ctaatgcgat agcgccggcg atatttggtg tccctgcttc gaatttccaa
    15781 ggtagttctt tccatgtact gtcgtataat tcgacaaaat cgatcatttc gccaccaaat
    15841 tcaacaggtt ccatttcttc taaccatttt cgtttaccat ataaaacacc aatacctgtt
    15901 ggtccgcaca ttttgtgtcc gctgaaagca aagaaatcac agtctagtgc ttggacatca
    15961 atggccatgt gcggggcaga ttgagcacca tctactacga tgaccccgcc gttttcatgg
    16021 acaagttcag ctaattcttg aacaggattg aagacaccta agacattgga tacatgagca
    16081 atggagacga ttttggtttt gtccgtaatc tttttcgctg catcctccat atccaggaag
    16141 ccttcttctg ttagttcgat atatttcaag acagcacctg ttcgttttgc caactgctgc
    16201 cacgggataa cattagaatg atgttccatg taagagagca ctatctcgtc tcctgctttg
    16261 atatagcggt cgccgaaggt tctggctacc caattcaagc ttgtcgtcgt accacgagta
    16321 aacagcactt ctgctgtttc ttttgcatgg atgaacgccc gtactttttc tcttgcttgt
    16381 tcataatcat gggtcgcgcg ttctgctaac gtatggacac cgcgatgaac attggcgtta
    16441 tcgtgttcgt aatagtacgc taatgcctgg agtacttgtt ttggcttttg ggtcgtcgct
    16501 gcattatcca gataaactaa aggttcatca ttgacgtctt gaaataagat aggaaaatca
    16561 ttgcgtagct ttttagcatc gatcatccat tcaacttccc ttcgattact tcgactaatt
    16621 ctttccgaac ttgttctaca gggattgctg ttaagacaga accgaggaat cctcgaatca
    16681 ctaagcgctc tgcttcttct ttatgcaatc ctctactcat cagatagtac atttcttctg
    16741 gatcaactcg tcctacactg gcagcatgtc cagcagttac ttcgttttca tcgatcaaaa
    16801 gaataggatt cgcatcacca cgtgctttat cagaaagcat caagacacga ctttcttgtt
    16861 gggcatctgc accttttgcc cctttcaaga tgtggccgat accattaaag gtcaatgttc
    16921 ctttttctcg gatcactccg tgttgcaaaa tatgcccgat cgtatgaggg gctttattgg
    16981 ttactcgtgt atcgattcct tggatttgtc tgcccgagct gattgccacg attttgactt
    17041 ctgcgtgtgc accttctcct gctaaatctg agtcaaagtc agcgatcaca tgaccgtcat
    17101 tcatgacacc aattgcccag tcgacagaag catctcgtaa aatgtgccca cggcgattca
    17161 tataagaagt aatattttct cctaattgat ctacggctga atatttgatt tttgaaccag
    17221 ctttagcgat gacttccacg atgatattac ctgatgattt tgctacttgt tctttggttg
    17281 tctggaaacg ttccagataa gaaaattcac tatgttcatc tgccacgatc aacacatgct
    17341 tgaagaagtg ctcatcggat gcgccatctt gaatgaataa ggactcgagt ggttcatcga
    17401 tcacgacatt tttaggcaca tacaggaaaa taccgctgtt cataaaagct acatggagtg
    17461 cagtcaattg gtcttcgttc atgtttactg cttttttcat ataatattct tggactagtt
    17521 ctggatattc gatcattgca gtgaacaagt cggtaaaaat gactcctttt tctgctaatt
    17581 ccacaggcag ttgttcaaaa atcgtccatg aaccttgttg gacaagcaca ggattgtctt
    17641 tcattgcatc aaatgccgga atcgtgcctg tttcactgat ggtttgtgtt tctttcacat
    17701 caaataacga ccagcgatgg aatttcaccc gatcgatatg cggcaatggc agttcatctg
    17761 ctttcgctag tgcgtcttga cggaaggttc tcatccaatc aggttcgcct ttacttaaag
    17821 aaaaatcatt taccgcgtca agataatcga gccagttttt ttctttcatt cttgctcccc
    17881 cttacgcttc ttcttcttta tagtcgattc ctaattcttt actgatacca gcgtagcctt
    17941 ctgcttccag acgtttagct aggtcagcat tccctgtcat gactactcgg ccttccatca
    18001 tgatgtgaac gacatcaggt gtgatatagt tcaataatcg ttgatagtgg gtgatgatca
    18061 atgcaccgaa attgtctccg cgcatttcat ttactccttt agcaacgact ttcaaagcat
    18121 cgatatccag acctgaatcg atttcgtcta aaattgcaaa tgtaggttct aacatcaaca
    18181 attgcaggat ctcattccgt ttcttttctc cgccagaaaa accttcgttc agataacgtt
    18241 cagccatttc ttctggcata tttagtaaag ccattttttc atctaacttt ttcaaaaatg
    18301 ccataacaga aattttgtcg ttttcatcac gtttcgcatt gatcgctgca cgcatgaatt
    18361 ccgcattcgt gatacctggt atctcacttg gatattgcat agctaaaaat aagccaagtc
    18421 gcgcacgttc atctacttcc aagtctaaaa catttttccc gtcaaacaaa atttcgcctt
    18481 ctgttacttc atagtttgga ttgcccatga tagcggcaga taaggtagat tttcctgtgc
    18541 cgttaggtcc catgatggca tggatttctc cggttttcat tgttaggttt acgcctttaa
    18601 ggatttcttt ttcctcgatc gacacatgta gattttttat ttctaagaca gacatgcatt
    18661 atacccccaa attctctttc gttctcactc attttagact aattgagaat gaagcgcaat
    18721 gaccttcctt actaaaatca aatatctttt atttattatt aaacaatttt gatttctaaa
    18781 ttagaatagg tataaaaaag atgagaaata agccattaca gacttatttc tcatcttttt
    18841 tgttccatac cttattttgc atcagccttt actggtttaa cagagccgcc ccatttgtct
    18901 gtgatccatt cttggacttc tttgcggtga agcacttcaa tcagtttttt gattttgtca
    18961 ctgtcttcat cacctttacg agtcgcaata atattcacgt aaggagagtt gtccttttca
    19021 agcaatacgg catcttctaa tggattcaac cctgcgccgt aagcgaagtt tgcgttgatt
    19081 gcaactaggt caccttcatc attttggtac gtagaagcta aaagtgatgg gtcgatcgta
    19141 tggttgaact tcaagttctt agggttttct tcaatatcat cgaatgttgc tgtttccaga
    19201 tctactccat ctttgacttt gactaagcca gcatcttgta gaattgtgat gatgcgtccc
    19261 cagtctgatt cagaatttga agtaatgatt gttgcaccat cttttaattc ggaaacatct
    19321 ttgacttttt tagagtacaa gcccattggt tcgatgtgga cagcaccggc attgacaaag
    19381 tcatatcctt tttctttgat ttctttgttc aaataaggaa tatgctggaa gtaattggca
    19441 tcaatgtcgc cttcttccaa agattggttc ggtaatacat agtcatcgaa tttgacgatt
    19501 tcaagatcga ttccttcatc tttcaataat ggtttcacat gttccaaaat ctctgcgtgc
    19561 ggtgtcggtg aagcgccgac tttcaatgtc gtgtcgtctg ctttatttgc tgaatctttg
    19621 ctgtcgctgt tgttaccgca tgctgctaat cctacagtca gcaaaagcgc tgctgcaaat
    19681 ccaaaaattt tctttttcat atcctacatc cccctatatc ctattatctt ttgtctgttt
    19741 tcttagttaa gaaatcaccg attccttgaa cgataaatac gattagtaaa ataattaaag
    19801 tcgcaacgaa tgtcactgtt agattaccac gctggtagcc ttcctgccaa gcaagacctc
    19861 caagtccacc tgcgccgatc gcgccggcca tcgcagtaaa tccgatcatc gagatcgttg
    19921 taactgttaa tccagaaatc aatgcgggaa tactttcagg tatcaagacc ttccagatga
    19981 tttcccagta acttgctccc attgcatctg ctgcttccag tacaccaggg ctgacttcac
    20041 gaaaagcaat ctctactaga cgggcataaa atggtgctgc ggataaaaca agagctggaa
    20101 tggctgcttg cgcacctagc atagtgccta cgattgcttt agtaaatgga atgatcaaaa
    20161 ccatcaggat cataaatggc gttgaccgga aaatattact gatgatcgat acgattccat
    20221 aaatgattcg agcatacggt ttgtttttgc gtccaataga gtaaaggatc aatcccaaaa
    20281 ctaatccgat gatcgtcact aagaccattg agattaatgt catatatagt gtatcaagga
    20341 tagcctgatt catcgtttcc gggttgattt gtttaaaatc tagatatgtt tctgaaaagc
    20401 ttttatccac gatggatcac ctcaattcct actccttgtt ctttgatgaa cacttctgct
    20461 tgatctaatt ctttggtttc accagtcatc aagacagtca atgaaccaaa ggaaccttct
    20521 ttcacttgtt tgatctttcc atacacaacg ttgacatcga tgttatagcg tcgaatcgct
    20581 tgggaaatga ctggctcgtt ggcatttctt tcgctaaagg tcaaagttgc caaacggccg
    20641 cttggatttt ctttgatgag ttcagctaat agttctgtcg aatcagcttc tggttcgatg
    20701 tcttgttgta caaagcgttt ggttacttct tttttcggtt gtctgaagac agtcaatacg
    20761 tctccttctt ctacgacttt ccccaactcc atcacagcga ctttgttgca gattttgcgg
    20821 atcacattca tttcatgtgt gatcaagacg atggtcaaat tcaatttctt attgatcgct
    20881 aacaataagt ctaatacttc ttctgttgtt tgcgggtcca atgcgctagt agcctcatca
    20941 caaagcagga tctcaggatc atttgccaat gctcgtgcga taccgacgcg ttgtttctgt
    21001 ccgccggaaa gttgtgaagg atatgcttgt ccgcgtcctt ctaacccgac taaacgcaac
    21061 agttcttctg cccgttcttt acgtttgctc tttggtacgc cagctaattc cagcggcagc
    21121 tggatatttt caagtacagt tcttgaccat aacaggttga aatgctggaa gatcataccg
    21181 attttctttc ggaatgtacg taattctttg ttttttaatt ttgtaatatc ttgatcgttt
    21241 acgatcactt cgccgctcgt gggaagctca agtccattca ggagacggac taatgtactt
    21301 tttcctgcgc cagaatagcc gacgattcca taaatatccc ctttttcaac gtttagtgaa
    21361 acgtcattga cggcttggat gtttccgttt ttcccattga atgtcttttt gacattcgtt
    21421 agttggatta atgacatgcc attcacatct ttctcttata atttttcaaa caaaaaagcc
    21481 ttcgccctct tgccttaagc aaaaggacga aagcttgaac ttccgtgtta ccaccttgat
    21541 ttaccgttac ttcgcaataa cagcctcttc cagtacttgg acttagcttc tttccgtata
    21601 ctgtcgcgct gtaacgggca ctcccgtagt aagtttgctt acgctcgctt actctgctct
    21661 aagaccatct tccatctcat tttcgttacc cattttcagc aaacagggct ctcttcaaac
    21721 gtccgtgaca tgtactcttc ttttcaaagc tttctcgttt catataggat tgcttatgat
    21781 tatagcaact ggaagaatca gtgtcaactt ttttcaagtg attttctgat tttataattc
    21841 gtcaaattct tttggatcaa catctttgaa acttaagatc cacgcattca tttcatctga
    21901 atcattcaag atattgatat cttcaccgat tttatcatta acagaagaaa ctgtacccgt
    21961 tagcgggctg gcaaattcat tgaccgcttt ttccgcttca acttcgatca gaggttcatc
    22021 tttttttagc gattgtcctg cttttggcag attggcaaac gtgatgtctc caagttcttc
    22081 ttgcgcttca tttgtcaaac caacacagta ttcttttcca ttaaataaga tccaaaggtt
    22141 gtcctttttc ttcaaatgct tgcctgccat tttatatcag ttctccttca taaatttttt
    22201 cattgaatcc aattgcaaca agctggtcct ctcgtaagat cagtggtcgc ttgatcagca
    22261 taccatctgt cgataagact tggctcgctt ttgaagccgt aaactcgcct acttgatctt
    22321 tcagtccaag ttcccgatat ttcatcccgc ttgtgttgaa caggcgacgg attggtactt
    22381 gactcgtttc tagccaagtc tcaatcgttg aggccttcgg tacttctttg atcatatcaa
    22441 cggtttgata actaacgcca tgctggtcta accacgcttt ggcttttttg catgttgaac
    22501 atttcgggta ccagtaaaag gtgtacaatt ttcttacctc cttgaagatc ttgcaggtgc
    22561 ttcattttgg tatctcatcg ataatatcaa cccgataccg atcatgttcc ctaaaatcga
    22621 ggatcctccc tgactaatga aaggcaatgg gatccctgtt aaaggaagta atccgatatt
    22681 tgctccaata ttctcaaaga catggaacag cagcatcatg atcaaaccgc ttgcgatata
    22741 ggcgtaaaat tcattattcg tatcgaaaca cacccgaatc atacgataga ttaaaataaa
    22801 atacaagaaa atgacgaatg caccaccgat aaagccgaag ttttcaccga taacggtaaa
    22861 aatcatatcg gactcacgaa caggcacata gatattgctt acgttgaatc ctttaccaaa
    22921 caagccaccg gtaccaattg ccaaaagtcc ttgggccggc tgataactca ttcctgacgt
    22981 atcatggaaa gggtccagcc atgaatcaat acgtgcaaac tgataagatt tgaatccaac
    23041 tttataaagc aagtctcttc cgccttcagt cgtaaccaag aagatcgttc ctgctccaac
    23101 gactactgcc aagattacta caggtgcgat gatgcgccaa gagattccag acatcaggaa
    23161 aatcccgcca aagatcgcaa gaaagacaag catcgttcca aagtcatctt gggccatgat
    23221 caatgctaaa accggtattg tcacgatcaa gagcttccca atcagccaaa agtccgattt
    23281 catcgtccgt tcttttactt gcgtattatg tttagttacg atcagtgcca tcatcaagat
    23341 gtacgcaatc ttcatcaact ctgctggctg gaaagtaaac gcaccgaatc gaaaccagtt
    23401 tttagaccct gttgaagcag ctaaattctg atcatagaat ttgagcaaga gtcccattac
    23461 cactaacccc aaaccataaa ggtaaggagt cagtttccag atccatttgg aattcaaatg
    23521 catgatgaca acgattgctg ccgctccaac taaataccag acaacttgca tccctacgcc
    23581 tttcaagact gctggattgt tcggatcatg gctaagtgct acatataatg agagcagacc
    23641 aatcaaacat aacagaaaga ctggtaaaat cacgccataa tcaatacgat tatcgttcga
    23701 agcttttaat tttttttcca tgtttcatcc cttttcttaa aaaccatact gctgattatt
    23761 atagcggtcc agttagataa tggaaagtct ttccatagac atttgggaaa aattaacttt
    23821 ccgtttgtgc agttgcttga gcaaatgcag tacaaaagcc tactgcttat ctttagtgta
    23881 tcatagaact ttttttgata catgcaatta tctaccagct gagattctcc tcgctgtgag
    23941 aagtttctgt gctttcattg tatcgaagga tctcttcggc aatctctagc agctgatcag
    24001 aaaccagatt ggttcgttgg atgaaataaa aatatcgatg gaaattcaac ggagtcattg
    24061 gcacattttc tggaacagag cgcttagaca aaatcgagcg accgattcct tccttcagca
    24121 acgcaacaat tacatcgtta cttttcacga tcatcttctt atcttggata ttcatctctt
    24181 caaaataacg ctgcgtgtaa tgaaaaacac cagagtttgc ttctcttaca agccacaatt
    24241 tgctttttgg gtccccagcg atgactagct ggtcttccat caaagagtat ctttgtacgc
    24301 cggaagtctc taatggcttt tcgataaaac caaaatcgga ttcatgtttc gctacttgtt
    24361 ctaatgattc atgagaattc gtgatttcga tctcaaaatg cactttagga aaccttttga
    24421 tcagatgttt catcaactga ggcaaaagat agacagcaaa ggtgtgggaa gcaacgatcc
    24481 gacatacttc ttcttcattt tgttcattga gcaatttttg ctgagtttct tcccattctt
    24541 ctaacaaaac cagtgtccgt tcatataata agtcggcttg cttagttgga agcatctctt
    24601 ttcttccgtt acgttgaaaa agatcgatat tcaattcccg ctccaactgt ttgatttgat
    24661 tcgagactgc tggttgagat aaaaacagta cttctgctgc tctggaaaaa tttcttgttt
    24721 catatactgc tcgaaaagtc gttaataact taaacatggc accacttcca taacttttta
    24781 tgataattac tataaaaact atttgattta aatatacaaa aaaccatcta taatagaaat
    24841 gtacaacaaa acagttgctg atccaactag taacttagat gaagacaatc agctgtctgc
    24901 ttgctcgtaa tcatacattc ctattcattt tacaatgtat tcgttcatct tggtactaaa
    24961 atctattaaa taaaaaggag aatattatat atgaagaact ttttgaaaat tattccgatc
    25021 ccgatctgcg ggttgatcct tgggttagct tcccttggaa atctgtttaa agactatcac
    25081 catgttgctt taggcaatct aactggcggt atctctatga ttcttatgat attcattttt
    25141 gttaaattgt ttatcttatt cgaacacacg aaacaaacgt tgcatgatcc aatcatcgcc
    25201 tccgtttcac caacttttac gatgtcactc atggttattt gtacttattt tgtttcatat
    25261 gaaagtattg caccaattgt caaattcatt tggctgtgcg ctgttatctt ccaagtgatc
    25321 cttgtctttt atttcaatta tcaccatgtt atcaaagctg atctttcaat tgaagcaatt
    25381 tatccaagct ggtttatcat ttacgttgga tttggggtca tcactgtaac tgccgggaac
    25441 ttcttcccac ttattggaaa aattttcttt tggatttcat tgatctgcta tgcgttctta
    25501 ttgccgatca tcatcaaacg tgtctttttc gtaagaaata tggctcatcc gacacgtccg
    25561 ctgattacga tcgttgcagc acctggttct ctttgcctaa caggttatct taaaaacttt
    25621 gctgaaccta atttttatct tgtcgtagca ttgttcgctt tatctcaagt attgtatttc
    25681 gttgtgctgt ctatgcttcc acgtttgttc aagcttcgtt tttatccaag ctatgcagcc
    25741 tttacattcc cgcttgttat ctctgcaaca gctatattta ctactgttca ttacttcaac
    25801 agtttaggta tctatagtag cgtattggat ttcttaaaag ttgcggaatg tctgtttgca
    25861 agtgtagttg tcttctacgt tcttgctcat tacgtacgtt tcttaattaa agaacatagc
    25921 aaacatggaa aatttgtttc taaaaaaatc gcttaataat cgattcaata ggttgtgaaa
    25981 aagctgttct gcatcaagta ataagaacgg ccaaacaaaa acagctgttc atgtttttgt
    26041 actggttggg cttattaccg cagatgcaag cttttgaaca ccgtttacca agaggtcgta
    26101 aaagaagtgg tcagcttcaa gaaataagaa ggaatttacg aaaattgctt ctcaaatttt
    26161 tgtgaaattt cggcttattt ccgtagaagc tacttctgga acaccgttta ttcgaaacaa
    26221 gaggtcgtac aagacttttg tcacgacctc ttgttctttt gtcaaaatac tggtttagtg
    26281 ctactatgtt cgagtatgct tttagtattt attgacgaaa ggaaccgatc aaatgaaatc
    26341 attgtcacat ccaataaaat ctgtaaagga ttcagcggtc cgcgaaatct tacccggttt
    26401 actgctttct tttgccatcg ctgccttcag caggcttctt gctatttatc tgccacagtt
    26461 aggtggagcg actattgcta ttttattggg tattttatta ggaaatactt tttttaaaca
    26521 gcctgttcta gcaatcggca caaaatttgc tgaaagcaaa ctattagaat tctctgtttt
    26581 tttactaggt gcaactgtca cagctcaaac gattgctcaa attggtgtaa aaggaattgt
    26641 ttttgtgtta ctgcaaatgt cagtcactat catcggtgct tacttgattg gtaaaaaact
    26701 gcaattcagc gatacttctt ctttgatgat ggctggagga aacgctgttt gtggttcttc
    26761 tgctattgct tcaatcgccc cttccatcca tgcgaaagag gaagaaaaag gacagatcat
    26821 cacactagtc aatctactgg gtactgtgat gatgttgact ctcccttttc ttggattaca
    26881 gttgtttggt ggaaatctca ttgaaaaaag tgcacttttg ggtggtacac tccaatctgt
    26941 tggtcaagta gtcgctgggg caagcttact tgacaattct gttgtcgaat tcgccatgct
    27001 atttaagatt ctacgtatta tgatgctcgt tgttgttgtc tatctgtttg aaaaattcat
    27061 gcacaagaaa caaccagctc tgtttgaaac gccaactgat acgcagaaaa aacgaaaatt
    27121 accgcttcct tggtatgtat tagggtttgt gatcttttgt ttggtcaaca gcttgttttc
    27181 tttacctgta gttattgacc aatcggcaca ttttatcagc agctggtttg agatcactgc
    27241 tttagcggca attggtctgc gtttagactt tgcaaaattc atgaaagaag gtacacgctt
    27301 cttatcttat ggattagctg tcgggcttct ccaaacagtg atggctttac tatttatttg
    27361 gctgatcaaa gtttagaatg aacatgaaaa aacagttgtg attctcgcat ggaacaatgt
    27421 ccgaatcaca actgttttta attttgcaga tatccttcta acaacaacaa ttggcgtttg
    27481 atttcaaccc catctattgc gtttcccata tatcctgtca gttttccatt ttgcccgatc
    27541 actcgatgac aaggaataac gattggcaat ggattggttc gatttgcctg cccaatcgct
    27601 cgcacagctt tcggactatc aacagcaacg gctacttctt tatacgttcg gatttctcca
    27661 taaggaatgg tcgacaaagc tttccagaca ttttgctgaa aaggcgtacc tctctttata
    27721 gataaaggaa catcaaattc ttttcttttt cccgcaaaat attcttcaaa ctgttcttgt
    27781 gtgcgcgtta aaattcgctg attcgctgat tcgctttctg aaattggctg aaaagataca
    27841 gcagtcaatc cggattcatc tgcatctaac cataaaaagc cgataggtgt gtgtattttc
    27901 attttgtttg ctcctagtca atagactgga acttcctcct ttttctctat ccaaaactac
    27961 ttgccaacta ataaaaattg ttcttaaaag tttgataaac cgaggaaaca tggtaaacta
    28021 aatacgttag tttacaaaaa gaatacctgt tcttttacgt aaaatcaaat tttaaaggtg
    28081 ttatttaagg tttattaaat tttgaatgta aaccatatat aaacatacga ttgaaaggat
    28141 agaaacgatg aaagtatcaa ttgttgtccc atgttttaat gaagaaaaaa ctgttcctct
    28201 tttttttgat gaggttgaaa aattcaaagg tgcccatgag ttcgaatatt tgtttattga
    28261 cgacggatca aaagacggta cgttagcagc aatcaagaag ctagcctcag cttatcctta
    28321 tgtccgcttt gtctcatttt ccagaaattt tgggaaagaa gcggctctgt acgctggatt
    28381 gcaagcttct acaggagatt tagtgaccgt gatggatgtt gatctgcaag atccgcccga
    28441 actgcttcct gagatgatca accggatcac tacttctgat atcgattgtg tcggcacccg
    28501 gcgttcgact cgtgaaggcg agcctgcgat acgcagtttc tttgcgaaaa aattctacca
    28561 gttgatcaat aaaatcagcg atacagaaat ggtcgatggc gctcgtgatt tccgcttgat
    28621 gactcgtcaa atggtggatg ccgtcctaga attaacagaa tacaaccgat tttcaaaagg
    28681 attatttagc tgggtcggat ttaaaacaga atatatttct tatgaaaacc gcgaacgtgt
    28741 ggctggcgaa acatcttggt ccttctggaa attattcaac tactcgattg atggtatcgt
    28801 gaacttctcg gatgcaccgt taacgattgc ttcatttatc ggcgctttgt cttgtgtcgg
    28861 ttctggtatt gcaattttgt tcattatttt gcgggcattg ttgtttggtg atcctacatc
    28921 aggatggcct tccatggtct cgatcttcct gttcattggt ggaatccaat tgttatgtct
    28981 tggaatcatc gggaaatata ttgggaaaat cttcttagaa acgaaacagc gtcctgttta
    29041 tatcataaga gaaacagaaa aagtgaagcc aaatgaattg aagcctcacc aataatatac
    29101 gtcaaatgaa gtaaacattt ttttaaatga aaaaggactg tgacaaaagt cttgtcacag
    29161 tccttttttc cgaataaatg gtgttccaga agtagcttct tcggaaataa gccgaaattt
    29221 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
    29281 ctatcacaac ctctttcata tcttattact tcttcattgc tgcttttagt gctgctgcta
    29341 atgggctttc ttctggttct tctgcttgtg catatttttt cagcaagcgt cgttcttcat
    29401 gtttggtcat tttcttcttc tgctcttttt tatcaagcaa tttttctgtc gttccatcgt
    29461 atttacaacg gaaataagca ccgtttttac cttcgatgat ttccattttc cggtgacatt
    29521 gcgggcatcg atgattagag acttttggat cttttcttct gcggtagttg caatctgaat
    29581 tggtgcagac atagattttg ccgtcacgtg tgtttttctc gcgcagattg gccccacatt
    29641 ctgggcattt cttttgggta atcgagaaat cttgatattc ttgtttgctt gatttgatct
    29701 cattcaccaa gcgttttgtt tctttttcga ttccattcaa gaaggttcct gcttgtttct
    29761 ttcctgcagc aatagcttca aggtcttttt cccagctttc tgtcaaatct ggtgttacta
    29821 aagagggatt taccaatact aataattgct tacctttagg agatacttgc agtccttgag
    29881 gggtacgttc catcagttca gatttgatta gtttctcgat aatctctgca cgcgtagctg
    29941 gggttcccag actgtatttt tccatttgac caagcaagct tgcttctgac aatggtttag
    30001 gcggtgcaga tagttctttt ttgatggcga aatcgggatt gattctcatt cctttttccc
    30061 atttgactgt ttccgtctca gtttcagttt cgcctttcca tcctgcttgg acgacacgat
    30121 tttgccgaaa aatgaatcgt tctttaccaa attcaaccgt aacttttgtt tggctgactt
    30181 tatggggttg agcaaacagt cctaaaaaac gagagacaat catttgatag atttttcgct
    30241 cttcattact taatttttca taacgtgctc tgttttcagt tggcaaaagt gcatggtgat
    30301 cggtcacttt actgtcttgg aatacttttg tctgttggac gatggctccg tttttcaaat
    30361 actgcttgac ctcaggagca aagtcgctga ctgcttgcaa tcgttctttc atcgtacttt
    30421 tcatgtcatt tgtcagatat ttgctgtctg tacgcggata agtaacgatc ttatgggtct
    30481 catacaaact ttgtacaaga cttaatgttt tctttgcaga atagccatag cgttgattgg
    30541 cttctctttg gatctctgtt aaatcataag gcaatggcgc agattccgtt tttaattttt
    30601 cttggacttc tgtgacagtt cctttgcttg tactgatcaa ttgaacaagt ttttgtgctt
    30661 cttcttgtgt ttttaaagca aattgatttt tttgaagcat tttggcttgt tcttttccag
    30721 cttgcaacgt gatcgtgaaa tacgtttgtg gacggaactg ctcgattttc ttttcttggt
    30781 ctctcactaa tgctagtgtc ggtgtctgca cacggcctgc tgataaatta tccgcgtact
    30841 tcacagttaa ggctctggtc acattcaatc ctaccagcca gtccgctttt gcccgggcaa
    30901 gagcagattc atacaatgca tcatattctt tcgcaggtct tagttgcttg aatccagttt
    30961 tgattgcttt atctgtttga gaagagatcc ataaccgctt cactggttta ttgaaatgca
    31021 catactctaa gatccagcga gcaacaagtt ccccttctct tccggcatct gtcgcaatga
    31081 ctgcttctga gacatctttt cgctgagcca gctgcttgat tgcttttaat tgatgtcctg
    31141 ttttaggaag tggtttgatt cctagttttt tagggatcat tggcaacgtg tccatttgcc
    31201 agctctgcca ttccttattc agatcttccg gcattttcaa tcctaataaa tgaccaaggg
    31261 cccaagtaac gatcaccttg ggtccttcat aataattttt gtgcttttga ttagcaccaa
    31321 gcaccttgct taggtctttc gcaacgcttg gtttttccgc tagaattagt tgtttcataa
    31381 aattcctttc tgctttctac tgtgatgggc tgatgatttt tcaacaacgc aagtcccttg
    31441 tattgctgta aatcatcgtc acactcttca caccagcgct catctccgtc attccaaaag
    31501 gcgctgagaa agttactttt tgcattgata tattcataat tttgatggat ttcgttccat
    31561 tgtttttcgt acgcttgtcg tgcttcttcg aaagactcga atgtttcttc ttcttcaata
    31621 tcttcttccc agtcttcaaa aaaccaccaa ggctcattgt ccccgaacat ggtaattact
    31681 tgatacatac tcgtgctcct tctttcagct tttctagact catcttagtc acttttcaag
    31741 aaagttgcaa gaattttctc ttataaaagg gtcatttaat gaacatctta aaattgttac
    31801 aaatgaatct gattgtttaa ggcaaagtag cgataaagtt attgatcatc caaaaatagc
    31861 tgtcttctgg atcgattgtt ggttcttttg taaagtatcc taaaaaacgt aaggaaagat
    31921 aaccgtggat catactacga agttccctgc ttcgatgaac tttttcttct ttcgttaatt
    31981 gaaaagcctc aaatatctgc aaaatgatct gattcgtttc atggattcca tctaataaaa
    32041 cagcttcatt ggtatgcggt acactgatca gtaactcata cactgcttga tttgaaaaag
    32101 caaaggatcg ataagtttct gcatagatct gcagtgcttc ctgaccactt tttcccacaa
    32161 gagcccgtcg cagctggtca ttaagttcct gcagaagttt cgctgttagc gctgttttga
    32221 catcacgaat atttttgaaa tggttataca gcgagggagc cttgatatcc aagcgtttgg
    32281 ctaaatgctg aaaggttatt tgctgaacat ccatctcttc tgctagttct cgaaagcagt
    32341 cgatgatttt ctcttgagac agatttcttt ttttcatttc catgtttcct ctcataaaat
    32401 aaatccttca ctctttcagt ttaccacgaa aaagaaagag aaaaaagaga ctttcattga
    32461 taaactacat attgtagttt ataataaaga gctgtagatg aaaattttca gaaagaagga
    32521 gtctaattgg ctgcaagaat aaaggaaaaa ttatttagga aaagagaaac ttgggaacaa
    32581 aacttgtttg tcttatggtt tggaacattt atggctggta tcggatttag tttagtcatg
    32641 ccttttatgt cactttacat agatacgtta ggtaattata gtacgtcaca attgaacttt
    32701 tggagcggat tgactttttc atctactttc ttggtgacta cgcttatctc tccttggtgg
    32761 ggaaaattag ccgaccaaaa aggaagaaaa ttgatgctgt tgcgtgcttc attaggaatg
    32821 gcaatcgtca tttctttgat gggttgcgtg actagtgtgt atcaattagt cgcattgcgt
    32881 ctgcttcaag ggattttttc tggttatatc agtaatgcca ctgcccttgt cgcaactggg
    32941 acgccaaaag aaaaaagtgg tcaagttctt ggaaccttag caactggttc tgttacagga
    33001 acattacttg gaccgcttct tggcgggatc tctgcttcag cgtttggcta tcgccctacc
    33061 tttttcatta cagggatcat cttatttttc gtcttcttgt tgagtctgtt tttcgtccat
    33121 gaaaaatttg tcccagtgaa aaaagaaaac atggtttctg ccaaacagat tttcaaagac
    33181 ttgaaatatc cacacgtcgt catcggtatg ttcgtcacaa cgatgatcat ccaagcatcc
    33241 aacaattcca ttagtccaat catcagtctg tacattcgcc aattgctgca tggacatggg
    33301 aatgttacgt tgatcagcgg aatcattgct tcgatacctg ggattgccac tttgatagcc
    33361 gctccaagat ttggtcgctt aggtgacaag attgggagtg aaagaatact tgcgattggt
    33421 ttgggttttg cgattctggt ttatatccca atggcattcg tcacgaatgt ttggcaatta
    33481 gctgccttac gttttttgat tggtatctct gatgcttgtc tgctccctgc tgtccaagca
    33541 ttgatcacaa aatattcgcc tcatcaagct gctggacgga tcttcagtta taaccaatct
    33601 ttccaagcaa ctggtaacgt agtagggcct atgatcggct ccagtgtttc cagtgcattt
    33661 ggttatcgcg gcgtctttct ttcgacttct cttcttgttt tggtcaactt cttgcttgta
    33721 cgacacaata cacaagagat ccatcagcaa gaaactgcag aagaaaaagc ttcaggagtc
    33781 tccatagctt cagggcactc tccttctcat agacaataag cgtaaatgaa aaacactgct
    33841 tgaacagttg tcacagactg tccttgcagt gtttttgttt aggcctgttt tttcaagaac
    33901 tcagataaga ggaataatcc cttttccaag gtttcttttt tacaacagta acctagtcgg
    33961 gcatgcttcg gtaaatcaaa ggcacttcct ggtactaata gtactcctgt ttcttctaat
    34021 aaattgatac aaaatgtctg gtcatcttct tcgatggcta atttaatgaa agaagtagag
    34081 acgtaattgg gagcgacaag ttcaactttc ggttcattgg ctacccattg ttttaaaatc
    34141 acaaggttct ctgtaatgat ctttttgttt cgcgccaata ttttttcttt attcttcaat
    34201 gcatgtacag caagatcatc tgacaggact ccgccgcaaa tcatcgtgta gtctcgatac
    34261 tttcgaaaaa cttctgccaa ctcaggtcca gtagctgtcc agccaatacg aatacctgga
    34321 atggagtagg ttttggatag ggaattggtt gctatacctt tttcatagac atctacgatc
    34381 gacaagaatt cacctttatc cgttaaaggc gcatatactt catctatcaa tacatatgca
    34441 tctactgtac gagcaatttc tgcgattttt tctaaagttt tccgatctag caatgtgcct
    34501 gtaggattgt ttgcactatt caaacagatg atttttgttt caggtttgac taaggcttct
    34561 agctgttcta aatcaaactg ccaatcttct tcttccttca aatgaacaaa gtcaacagcg
    34621 gcacctaaag atttggggat gtcatataat tgctgataag taggaagcat cgaaacaaca
    34681 tggtctcctg cttccactaa cgcgtataga gctaaaagat tagcaccagt cgctccattc
    34741 gtttgtaaga tgttttccgg atcgacggtt tgataaagag aagcgacttc ttgtttgaaa
    34801 acatctgatc cttcgatcca gccatagtcc aatggttcgt ttttgcgttt ttcgaaaaat
    34861 ttacttacgc tagtcccatc taagccaacg agttcgtcta atgtcaatgc ttcgatcgag
    34921 ctttgtgcaa tatcatatac cgctttcttt tcccatttat ttagccattc ttctacaccg
    34981 aaatttgcga ttttcatgtt tctttccccc ttatatcttg atccagccta cagcaagcaa
    35041 cccgattgaa agcagtgcgg ccacacaaat cagcccaatc attacttttt cttttcccgt
    35101 aatttgatgt ttggtttcac gacaagcgat ccagtagaag caaaaaccag gaagataggc
    35161 aatactacat aaaaggacat aagaaaatcc tgctaaaatg atgcccatca attcaaaaac
    35221 tacagcaatc ccaccgatca ctacttgttt tttgattcct tcttttaaag aaaactggac
    35281 ttgatagatc ccgacaagta gataacaaat caaaattgcc gctgtacata aagaataggc
    35341 aaattgatag gcataatcag taactaagaa agtcaataag aatagattag tcaagattgc
    35401 agtaacaact aacgaataag ttggggcatt ttttttattc gtttttcccc aacctttcgg
    35461 caacagaccg tctcgagcca tcaacatcgt tgtttctgct gggagcatcg tccaagacaa
    35521 ccaagaacca atgatcgaaa taatcaagcc gatattgatc agcgctgctc cccaatttcc
    35581 cacgattcct tttaaaatat ttgccatagc aggttgtgaa atagttgcta actcttcttg
    35641 agataagacg ccgtatggca agatcgaagc aaggatatag ataaacaata aacaaagcag
    35701 accaatgatc gatgcttgct gggcttctga acgtttcttc gcgcggttgg ctaaaacact
    35761 tgcgccttcg atccctacaa agacccacat cataaccatc aaacaacctt taatctgcaa
    35821 ccagatcgtt ccttgctgcc ctgttccatt cactagatta tcagatacgt taccccaaaa
    35881 atcagcagtg aatatattca ctttaaatgc gataaataca aatattaaaa ataggaataa
    35941 tggcactaat ttacagatcg tcacgattgt attgatgacg ctcgctcctt caactccatt
    36001 gtttactaat aaagtcagac cccaaataaa aatacttgca aataaaatag aaggaacatt
    36061 ttgtccacct ttgaatatcg ggaaaaattc tcccatggcg ctcatcagca tcgttgcaaa
    36121 agcaacattt cccagccacg cagacaacca gtaaccccaa ccactgatga agccacctaa
    36181 tttaccaaat gaagcttccg cgtaaccgaa aataccacca tctagatctg gacgcttttc
    36241 tcctaaatga ttcagcgata ataccaaagc taaaatacca atcccaacga ttatccatga
    36301 aaggatcgct ggccctggag ctgcagaact agccagatca cttgtgatcc caaacacacc
    36361 gcttccgatt gacgaactga caaccagagc aaccagtccc catttgctaa tacccgtcga
    36421 tttcttttcc atttccatca ccctcactta taaaattgaa caaatcactt gcaaaagatt
    36481 cccttctatt ttgcaactgc tcttgcttaa caaaaagcaa ctttattcat attattggat
    36541 attatgaact ttttacacaa gtatttcaag tataaaatga ataaaaatta attttattgc
    36601 aaaaaaacag ctatgctatc caaaagaaaa ggtgtttctt ttgttagcca tagctgtttt
    36661 gattcattta atatcgctta aacgtattgt aaaacttttg tttcttgata tgcgtggtcg
    36721 atcaaaccac cacccagaca ttccattcca tcataaaata caactgcttg tccaggagtg
    36781 attgcacgta caggttcgtc gaatacgact tctgcacgat tatcatccaa taagcgaact
    36841 gtcacaggaa cgtcttgctg acgataacgg aatttcgctg tacacttgaa ttcttttggc
    36901 attggttcat tcgtcgtaaa gtggatttca cttgcatcca agcttgtggc atacaatgca
    36961 ggatgatgga acccttgccc cacatataat gtgtttgtcg ctagatcttt tcctacaaca
    37021 aaccagggtt cttgcgaatc tcctccgcca ccgatcccta gtccttgacg ttgaccaatc
    37081 gtataataca tcaagccagc atgttgtcct ttgacttcac catcaagagt gaccatattt
    37141 cctttttttg ctggcagata gttgctcaaa aattgtttga agttcttttc accaataaag
    37201 cagattcccg ttgaatcttt tttcttagca gtcgcaaggc ctgcgcgttc agcaattgcc
    37261 cgtacttctg atttttccat gccgcctaga gggaacatcg tttttgaaag ctgttcttgt
    37321 gataattggc taaggaagta agtttgatct ttattattgt ctactccacg aagcatgtgg
    37381 accacgccgt tttcatcgcg agtgacttgc gcataatgtc cagtagctac ataatcggcg
    37441 ccaagatcca ttgcataatc caagaatgct ttgaatttga tttctttgtt gcacatcacg
    37501 tctgggttag gtgtacgtcc tgcgcggtat tcggctagga aatattcaaa tacacgatcc
    37561 caatattctt tttcaaaatt cacagagtaa taaggaatcc cgatttgagc agctactttc
    37621 gctacatcct tataatcttc cgtggccgtg catacaccgt tttcgtccgt gtcatcccaa
    37681 tttttcatga atattccgat cacgtcgtat ccttgttcct ttaacaaaag tgctgttaca
    37741 gatgagtcga caccgccgct catccctacg acaacgcgtg ttttgctgtt gtctgtcatg
    37801 atatcaccat catttcaatt agattctgaa acatctacga gttgaaggtc gcaacttttc
    37861 agtattttac attatacgtc atatttctga aaatttcatc ccttaattat caagtcgtaa
    37921 aaatactgtt ctactggacc tattcagtac acaaacatga tagtcaacca aaaaatcact
    37981 catcttttga gcattcgata taaaaaattg gcatactcat aaaaagggcc agcctttcgg
    38041 caataaggaa taattttata aattgtgaaa aactattttt caaaattgct tcttatcgct
    38101 taaagctgca cgtctcattt acaacctttt ctttttagat gttatgagtt gctcagataa
    38161 gtgattttgt ttactctaag aaaaaaggga ggcaaaagat acatgaatta ctttatcagt
    38221 gatatgcact ttttccatga acgcttatta ggaaaaaacg actttgctcc gcggctcttt
    38281 gcaaatgtgg atgaaatgaa ccaacagttg atcactagct ggaatgcagt aataaaagaa
    38341 accgaccatg tttatcatct aggcgacctt gctatgcatc cgcaatatga aaaagcaaat
    38401 gcagaaattt tggaactggt cactcaactg caaggaacaa tccacttgat caaagggaac
    38461 catgacagtc gcgctttttt ccgttattta gaaaaaaatg atcctaggct tactgacgga
    38521 agtcccaaat tttattttta tgatgtaggt gccatcgtta aattcgacca tcaacaatat
    38581 tacttgactc attatccgat gttgctcggg cctaacgaca ctatccgcaa tcttcatgga
    38641 catatccatc attacagcgt acctatcgag aacgatgtca atgtaggcgt agacgcaccg
    38701 gaaagagaac tcttaaagga aaaactacca tttggcactc cgttatctga gcaacaaatc
    38761 aaagaaatct atgaagcaaa gaggaagtac gataggtcgt gaaaaaactg ccctatatca
    38821 agtattaaac aaataaagat aattactcat atttttcatt tgagtaggct tattgccaca
    38881 ggaacattag gatcagaaat ggagttgtaa caagacttct gtcacaactc cattccatac
    38941 tattcgtctt tttgctcatg gtgttctttt accagttttt tctgtaattc tttatcataa
    39001 tttcgggcag ctgacgttaa aaataaaacg attccgcata ataaaatccc gattccggaa
    39061 aacaagaaca ttttttctac ccctaaacga tcagctaatg gtcctgcaaa taataatcca
    39121 attggacctg ccaggttcat caaagagttg aagttcccta agacacgacc taaaacattc
    39181 gattcataac tctgctggat cattgccatc agcagtgtat tgaaatatgg cgttgcacaa
    39241 cctgctcctg cattcaagat aagaaagtac aagaaccctt gactatcgtt tggaaccaat
    39301 ccgcttgcac cgatcgtcag gcccataatg atgtaggcta taataatagg tttcattcgg
    39361 tctttccatg ttcctataaa actgatcagt gcaccgccta agagcattcc gactgcgtag
    39421 actacttcca ctgctcccgc ctgtccaacg gaaccaccaa agtaatctag cgtcatcaat
    39481 ggatacagac tgattgctgg catgaacaac agcatcacaa atgcaccatt gatcgttatg
    39541 taccataacc ctttattatc catcaattgc tgcagcccaa attttgcatc tttaaaaata
    39601 gtaagcggaa cttccaatat ttcaggagaa acttttggga tcttcacaaa aatcaataag
    39661 ccgacaccaa aaactgcccc caatacatct aataaaataa ggtaattgac aggaacgact
    39721 gaaaacaaag ccgcacctag agctggcgca atgatgaaat tcgctgattg gaccattcct
    39781 aactgtccat tcgtccttgt tatgtgagaa gatggaacga ttgtcggcat aatcgactgg
    39841 atcgttggca tttggaatgt ttgtgcgacc gaacgcataa acaaagacac aaaaactagc
    39901 caaatcggga aggattctga aatcgttccg actactgcta aaataagcgc gaaaatcgct
    39961 acaataatat ccgtgacaat caaaagagct tttttatccc agcgatccac tagcggtcct
    40021 acaaatgggc ttagtaaaac catgggaatc attcctagta aagtagcaaa actcaaaatt
    40081 gtcgccgaac cggtttctct tgtcaaatac cagatgatcg catattggac gaccatacta
    40141 gtgattcctg acaaaaactg tcccgataaa aagaacaaat aatttctctt ccaattcgtt
    40201 aaaacgaccg actgttcatt ttccataaac atccctcttt tctttttcat tttgtatagc
    40261 gtttgcacaa caatcattat acgggattct tttttcaaaa aatacctttt tccctaaata
    40321 acaatcgttt caatttaaat ttctttactt agaaacaata aatcagcaaa atattaaaaa
    40381 taacaacatg tgttaaattt aaaaataaaa aagacaacta atttgcgtcg tccttacaaa
    40441 gttagtcatc taacaatagg ccgtttcaaa actagttgtc tttttatatc cacagatcat
    40501 tcgacttttt cgaataaccc acggctaatc atcccatcca tcagaaaata aaatttatct
    40561 tggctcattt tctgttttgg atctttgaaa atattgactg ctttcaatcc atttgctgta
    40621 gtgatcgaca ttttcaatgt ttgaatatct tttgatacag tgatttttaa tttgaatcct
    40681 tctttgcttt gcggcgtggc atctaatggt cggatcaatt gataattgcc tccgtttgtt
    40741 tctgtaaatc cattatcgcg caatgtatat ctttttgcat caggatgcaa acgatagcta
    40801 attggtgcgc ccagtaccgt tgctgttgtt tcaaatgcca tgattttcac ctcgataaaa
    40861 ttagtttctc tttattataa aagtttatcc tgatttttac taggtttttc agttaagttc
    40921 cctaaaaaat gaaggcgttc tctcagacat ctgttcatat aagaatgact tcactagtta
    40981 ttttttgaca gcacgaacta attgatcaag gaaatactgg atgtcgtctt cggtatttcc
    41041 caaaccaaag ctgatacgga tggattcttt tagtatcggt gtatcttctc catacattgc
    41101 ttctaatacg tgtgaaggct cgatatttcc tgctgtacaa gctgaaccag tagaaatagc
    41161 gaccccatta agatcaagct tcatcagtag caaatcattt gcttcgccaa ttaagcgaag
    41221 attcaacaca tgagggagtt tattcgttaa atcaccattt acttgataag ggatttcgtt
    41281 tgcttctaat gtatcaagaa tcaattgctg gaatccttta tatttgttgt tacgtgcttc
    41341 acgttcttca ggtgacagca gttcaactgc ttttgccata ccccagatcc ctgctaaatt
    41401 ttctgtacct gcacgccgct tttcttcctg ctccccgcct aaaagcaatg cagggatatt
    41461 tactccgtct cgtttatata agaaaccgac gccttttggc ccattgattt tatgagcaga
    41521 gatacttaat aaatcgattc ctaattcttc tggatggatc gtttggtttc cgtacgcttg
    41581 taccgcatct gtatggaaat aagctggatg atccgctaaa agttcaccga tttctttgat
    41641 tggcatcaaa tttccgattt cattgttccc gtacatcaca gagaccaaga tcgtatcttc
    41701 tcgtaacgct tttttgacct cttcgatcga cagattccct ttttcatcta caggcagata
    41761 tgtaacgtca aacccctttg tctctaaata tttcatggta ttaagaacag caggatgttc
    41821 gatagctgta gtgattagat gtctgccttc tttttgacgc gagaacgctg tttctaagat
    41881 agctgtatta tccccttctg ttcccccact gttaaagatg atctcgtgag gttttgcttg
    41941 cagactgtct gcaatgattt gtcttgctgt ttccaatttt tcatgcgcgt ggcgaccaaa
    42001 tccatgtatg cttgaaggat ttccaaacgt gtcttgcatg attgccgtca tcgcttcaat
    42061 tacttgtgga tgcatggggg tagttgccgc atgatccaga taaatagttt tcacttcatt
    42121 catcccttcc aaattattgc taggcacatt ctagcataaa atagcgaaca aaccaaagaa
    42181 gagctcattt ttgtctagag gacgtttcat gggcatagaa aaaaggagct gtgacaaaag
    42241 tcttgtcgca gctccttgaa gctgctcact tctgtcacaa cttctttccc tctttttttt
    42301 gtttattcga attctttttc ttccagacgg aataacggac tcatcggtgt attttcatgg
    42361 atgcgtttga ccgcttcacc cattaattct gaacatgtaa cgattgttaa gttttctgga
    42421 tgacgatttt cttctgtgaa aacagaatca gtgatacaaa tatctttgat tggtgcattg
    42481 tctaaaatac ttttcgcatg atcggacaat aatccatgtg acgcacaggc atatacttct
    42541 tttgcgccgt tttcttttaa tacatttgct gctcgtgcaa gcgttgctcc tgtgttcaag
    42601 atatcatcta ccatgataca tgtctttcct tgaacatcac cgatcacata accaccctca
    42661 gttgaagcat catctgcatg atctacgatg gctaatgtgc tgttcaaata ttctgaaaga
    42721 ctacgtgcac gctgaacacc gctgtttttt ggcgaaacaa ctacgatgtc atctcccgtc
    42781 agacctaatt gacggtaata atgagcaaac aacggcatcg taaataaatt atctacagga
    42841 atatcaaaga atccttgtac ttgaaccgtg tgcagatcta aagtcagtac tcgagttgcc
    42901 cctgcttctt cgatcaagtt agcgatcaat ttcgcagtga tcggttcatg aggtttcgcc
    42961 gtgcggtctt gcctagcata cccatagtaa ggcaatacga cattgatcgt tttcgcactt
    43021 gctcgcttca ttgcatcgat catgatcagt agttccatat agtaatcatt gacaggtgca
    43081 ttcgttgatt gaacgatata cacatgatcc cctcgaatac tttcttcgat attgatggaa
    43141 atctcgccat cactaaattg cttcacgacg cttttaccta attctgttcc gaacactttc
    43201 gcgatttttt cagctaatgg acggttgcca ttcaaactaa aaatcttcaa cgtgtcatcc
    43261 tgatacttat tactcatgta gtcctcccca aaacttctct attcttcgat agtttaacat
    43321 atttttccaa gattttctgt taatttcagc aaattcttaa ggaagtgaaa ctgttccgtt
    43381 actccaactg gcaggaatga ctttatgata atcgccccag ttgtcgtgta ctttgtaata
    43441 acgttctcct gtttcagttt caaaataagc gtacgctgtc acccaatgat tgccatatgt
    43501 actgccaagt actttcaaaa taccaatcat cacaggtttg ccttcacgta tacgttttgt
    43561 agctcgctgc catgaaccta gaagagttgc tcgtgcacgc tcatgattgc cattcttctt
    43621 taaaaaacta gaaatccctg tactaacctg aaaaggaatc gtaggaagtc ctagtggttg
    43681 gatctggcta cgcaatgcct tcgtcaaaac caacgaatca gccgtatttt tcttgcgaat
    43741 ctcatttgga agcatccacc ccttacgata gtcttgataa taggcaagga gaactgctgc
    43801 cgcgtaagta ccacaaagat agcctgaggg ggtagcccat ttcttatacc tcaaaaaacg
    43861 ttcttctggc aatcccaccc agcaatttgc gagttccttt cctgaccttt tttcttcgat
    43921 caaaacggga atatcttctt ttctgttgat ccatctagat aactcgattg gctcttcatt
    43981 gatcagcttc cattttttac aagaaagacc ctcttgataa gtatattttg ctggagaata
    44041 ttctaccgga tgtcctgatt gatcgataat cacataactt tgatcttcaa aaaccagagc
    44101 taaaaaaagc aatttacttt ttcttgactg cagctgctga gtgctggcta attttttaat
    44161 ttttttatct actttgaaat ataaaggttt atccactact tgattcagtt gggatcgcct
    44221 cctttttttc attataagtg aaggattgaa aaaaacaaat cagtttgatt gttcgaattt
    44281 tcaatttttt atgttctatt tgttaattcc ttcattttgt aagataatgg gcacacgaaa
    44341 caaaaaagga gcgatcgatg aatgaacaaa gaacgattga aacaagattt ctatgaagca
    44401 gtcaatgaag aatggcaaaa aacagcaaaa atccctgcag ataaaccagc tacaggcggt
    44461 ttccaagatt tagttgacgg gatcgaccaa cttttgatgg gagaaacaga cgatctgctt
    44521 gcccatccag aaaaagtgtc ttcagaaaaa atgaaacatt tccttgcata ttatcaactg
    44581 gcaaacgatt atgaccgaag aaatgagttg catgctcaac cgcttcaacc tataatcgaa
    44641 aaagttgaat cattgcagtc tttcgacgaa ttgaataagc aatttccaga atggctgttg
    44701 gatacattac cattgccatt taatctggat gtagatgcag atatgaagaa tgcgcaaacg
    44761 aacgccttct ttgcttatcc gccttcttta ttccttccag ataagacgta ttatgcatca
    44821 gaccatccca acggtgcaca actaataggc gttttctttg atatggctgt tcagttatta
    44881 gagcttgccg gcaaagaaaa aagtcaggca gaagcaatca cggaacaagc agtgcaattt
    44941 gataaattga ttgctcctca tgtaaaaagt gcagaagaaa acgcagatta cagcaaaatg
    45001 tataatccgc aatcttttga tacatttatt aaatacacag ataaactgga cttgaaaaaa
    45061 cttgtcacgg gtttgattgg gacaactcca gataaagtga tcgtaactga tccagtgtac
    45121 ttcgatcatt tgaatgacat cctgacagaa gctcattttc cgttattcaa aagctggatg
    45181 atcattaaaa cagtacgttc tcttagtggt tatctatcag aagaattccg ccaagtcagc
    45241 ggaatctatt ctcgcgtatt gtctggaaca gacgaagcaa tgcctcagaa aaaagcagcg
    45301 tattaccttg cctctggaca attcgatcaa gtagttggcg attactacgg aaaaaaatat
    45361 tttggcgaag ctgctaaaaa agacgtagaa caaatggtga aaaaaatgat cgccgtttat
    45421 aaaaaacgtc tagaagaaaa tgactggctt ggggatgcaa caaaagaaaa agccgtcata
    45481 aaattaaata acttaggtat ccaagtaggc taccccgata agatcccagc attattcgac
    45541 cagttcaaaa cagtacctgc agatcagggt ggtactttgc tttcaaatac tcttgttttc
    45601 agcaaactca cattaaaaga tcgtttcagc aaatggaaca aaccagtgga tcgttcagaa
    45661 tgggaaatga gtgctgacac ggtcaatgct tattatcatc catttcggaa tgtgatcgtc
    45721 tttccggcag cgatccttca agcaccattt tacagtctag aacaatcaag ctctgctaat
    45781 tatggtggaa tcggagctgt tatcgctcat gagatttccc atgcatttga taataacggc
    45841 gctttatttg acgaatatgg caacttgaac aactggtgga cagaagaaga tcttacacaa
    45901 tttcaaaaca aagcacaagc aatgatcgaa gaattcgatg gtctgccttt tgctgacgga
    45961 aaagtaaacg gaaaattaac cgtttcggaa aatattgctg atgcaggcgg tctaagctgt
    46021 gcattggaag cagcaaaaac ggaatcagat ctttctatcg aggaattctt tgtcaattgg
    46081 gcaacgattt ggcgtacaaa agcaaaaaaa gaataccaac aacttttact gcaaatcgat
    46141 gtccatgccc ctgctaaact ccgagcaaac gtgcaaccaa aaaaccttga cgaattctat
    46201 gaaacatttg gtatcacgga acaagacgct atgtatctag cgccagaaaa aagagtgcga
    46261 atctggtaag aggttgtgcc agaagtggtc agcttcaaga aataagaagg aatttgggaa
    46321 aatcgcttct caaatttttg tgacatttcg gcttattttt cgaagaagtt acttctggaa
    46381 taccatgtat tagaggttgt gaaaaagctg cttagtttca agtagtaaga aaaattttgt
    46441 gaaaatggct ttccacattt ttgcataaat ttgacttact accgagaaac tggcttttga
    46501 acaccgttta tcgagaggtt gtgaaatcag cgtcctgacc tgagtattag agaaaaaaac
    46561 ggaaaaatag ttcctcatat tttgccgttt gttgagctaa taccgagggt cagctgattg
    46621 aacaccgttt agtggagaga ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc
    46681 aatcaaaagc agcaaggaga acctcatcta taaaaagata gaaggaagtg agacatctat
    46741 tggtctcatt tccttctatt tttactttcc tcagtcaatt gttttacatc ataaaaggag
    46801 aaattccact tatttttttc gctgtttcat gacataaaca actacaccga ttaacaaaat
    46861 tcctccagct aatgctagca acgggttatt aaattctccc gtttttggga gtccttgagt
    46921 gccatttttt tcactttctt ttgctgtcct atccggatga gaaggttttc catttggttt
    46981 tccatttggt gattcatttc ctggattttc tctttctggt ttgtcatttc ccgggggcac
    47041 atcggcagtt tctcgcacag tgatcgcttt ttgctgggta acagattctg tatgagcaga
    47101 ccatttttcc ccatcaaata tttccgaacg ataagtgact tctagcgtat attctccttt
    47161 tgaaggtgct gaaaaaatag cggtatgatt agaaaattga gcacttgact gtgtttcaac
    47221 gatttggatc ttttctggga aataacgcat atcttcaaag acaggcgttc cttgatttgg
    47281 tgtgaaagtt gacacaaaaa ctgtaaaagg cttcagttca atgatatttc cttctgcatt
    47341 ctccgccaca ccatttgctt cttgtataat aatcggatta ttttgttcat ttggttttgt
    47401 ctgatttggt tcttgtacga ttgcatactc tggatattcc gaagacggtg aggtttctcc
    47461 gtcaaaaata gcacctacag ataatggaag accatccact ctttcctttt ctgtttgacg
    47521 attccccggt ttaatctgca tcagtccatg aacatggata cttccatttt catagcgcgt
    47581 aacaggctgc tgaccttctt tcgaacatgt atttagagac tcgatccaat cttttgatat
    47641 ggtatgtcca agagtatttt ctgattgttt ctctcgttca ttccaatagt agttcaacga
    47701 gtggtagatt tgatcttcgt tttgattcaa tgggcggatc tgctctctca tctcaagatt
    47761 ctctgttcgg aaagataaga taccagaagc atggtagtct gtgtttcctg ctgaagaggt
    47821 ataaagagcc acattatatg aaccgttatt aaaacttgta ctgctattga cgataatatc
    47881 tgttccgcta ttggaatcga ttcctttcgc aagattttcg aaagacaaac tattgatcaa
    47941 ttcatgaggg cccgttaaag aagaaccgcc cattttgaat ccattaccgt taccttctcc
    48001 ctcaattcct ggtacccagc cgtttcgata agcaactgag tttttgatga ttacttttcc
    48061 gatcgaacct gtttcatttt tagcaaaaag gtcccaaccg tcatctgcat tatgatacgc
    48121 gatacaaccg tcaaatacat ttccttctcc tattgtcagt ttggccgcaa atccatcggc
    48181 atcttcaaat cctggatcgg cattttcaaa agatgtacag ttcttgacta agttatgcgc
    48241 gggccatgct tcgaaaggat catttgagct accgctgatt tggattcctg tatttccatt
    48301 ccgataagca tgaatctctt cgatcaaatt atgatttcca gaaatctgta atcctttttg
    48361 catagctgca ctatttgtaa tgtcaaaccc tcgtaaaccc cagtagtgac tgaacagtgt
    48421 catcccattt ccagttcctt taaaatcaag tacaggacgc ttgttttctg gatttttctc
    48481 tgcaatcaac tgaatcgggt tttctcttgt cccgttaacg ccttttggaa tcgtcaatcc
    48541 tctttcgaag gaataagttc caggcgccat gacgattgtc tgcccagcat atgcgtatct
    48601 caatgcttgt gtcagagata atggattttg tctgcttccg tttcctgcat ctgacccttc
    48661 tggtgttaca taaactgtcg aatatttagg tttacggtag tccactgtat gaagaatctc
    48721 ttttgtttca taactagtca tctctacgta ttcttctggc ggatagtttt tatctggcgt
    48781 aaaactgaaa gtaaattcgt tcgttccttc catcagtttt gtctgataat caaattcccg
    48841 attcgcagtg atagccaagt tttttgcgtt cttaagcacc tctcctgttg ctttattttt
    48901 aattgtcaat aatccgtcag catttgctct aaaggacata gtatgatctt ggtttcctga
    48961 atgattacta gaggtcacag tgtaaatcgg atcaatcaag cgagtaggtc tttcttgccc
    49021 aggaggatct gtcgcaggat ctgagtaagc aacttcgatt ccttctacct tgatcgtcat
    49081 attccttgct gcagcaaatc ctgcataaat aaccgattca tctgaagcgt ataatttttc
    49141 ccagtcatac atgatgtcag aagccatttc ttctcctgtt tcaggatcgt aataatatgc
    49201 ctctaaacca gtattcgttt ttttcatctc tacgtccagt ttagtaatcg agttcgtggc
    49261 cggaaacaga ttcccatgac cgtttccgaa tatattataa gaacctgttt ctttttccaa
    49321 gaaccctgct gaagtctcta aaggtgttgt ttccactgag acactcccag gagctggcgc
    49381 agcttgcgga tcttcagatg atattcctgt gattgcccgt gtccccaatc caagtcgcat
    49441 cgtatatttg ctgccgctcg tgttgaccac ttcttgtcta tccgagtccc agacatattc
    49501 gatccgactg cctagtatgg caaatgaatt tgaataaaat gcctgcccaa acaaatgatt
    49561 ttttggtact gcgtctctta ccattaaagc aaatccttct tgggcattcg tataggtcca
    49621 agattctatt tcgatcgtcg cccgcatcct aaagttgatg tctttgggga ttgcttgata
    49681 ataataggtc attccatctg atccgcttga ctggaatttt ccgccgttat tcgtggaaga
    49741 gagccgcacg ccgtcttcta atggttcaat ggcataccta ctagcacttg tgctttctcc
    49801 gaaaacaacc cctttccact cttcatttgc ttcttcagat tgcttttttg tcttttcatt
    49861 ttctgattct ttcatcatag gatcatttcc ttcttccgta tccatatctt cactagttct
    49921 tggtatttcc tccattgttg acatcccttc aatgttcacg tgtgcatttt cttcgttcac
    49981 agactgttgt tcgttagcag caacttcaac gacagaaatt aaaaccgggg tactgagcat
    50041 aaggagattc atcattagcg cactgtattt tatacgtttt tttaacaagt gtattcctcc
    50101 cgtaacaaat tagaaagcgt ttacttaaag tattacctaa tttattttta ctgtcaataa
    50161 aaaac
//
LOCUS       DAYYKN010000005        50156 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000005 DAYYKN010000000
VERSION     DAYYKN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.005"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(240..1940)
                     /locus_tag="ACHBB7_000218"
     CDS             complement(240..1940)
                     /locus_tag="ACHBB7_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208203.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2447..3607
                     /locus_tag="ACHBB7_000219"
     CDS             2447..3607
                     /locus_tag="ACHBB7_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9208204.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3623..4066
                     /locus_tag="ACHBB7_000220"
     CDS             3623..4066
                     /locus_tag="ACHBB7_000220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9208205.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4549..5079)
                     /locus_tag="ACHBB7_000221"
     CDS             complement(4549..5079)
                     /locus_tag="ACHBB7_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM9208206.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            5184..5735
                     /locus_tag="ACHBB7_000222"
     CDS             5184..5735
                     /locus_tag="ACHBB7_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9208207.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5888..6361)
                     /locus_tag="ACHBB7_000223"
     CDS             complement(5888..6361)
                     /locus_tag="ACHBB7_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9208208.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6499..7971
                     /locus_tag="ACHBB7_000224"
     CDS             6499..7971
                     /locus_tag="ACHBB7_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM9208209.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7968..8678
                     /locus_tag="ACHBB7_000225"
     CDS             7968..8678
                     /locus_tag="ACHBB7_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM9208210.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            9030..9314
                     /locus_tag="ACHBB7_000226"
     CDS             9030..9314
                     /locus_tag="ACHBB7_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM9208211.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(9631..10014)
                     /locus_tag="ACHBB7_000227"
     CDS             complement(9631..10014)
                     /locus_tag="ACHBB7_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM9208212.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(10016..10600)
                     /locus_tag="ACHBB7_000228"
     CDS             complement(10016..10600)
                     /locus_tag="ACHBB7_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM9208213.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(10647..12122)
                     /locus_tag="ACHBB7_000229"
     CDS             complement(10647..12122)
                     /locus_tag="ACHBB7_000229"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM9208214.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            12303..12614
                     /locus_tag="ACHBB7_000230"
     CDS             12303..12614
                     /locus_tag="ACHBB7_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208215.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(12729..15065)
                     /locus_tag="ACHBB7_000231"
     CDS             complement(12729..15065)
                     /locus_tag="ACHBB7_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM9208216.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            15207..15875
                     /locus_tag="ACHBB7_000232"
     CDS             15207..15875
                     /locus_tag="ACHBB7_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9208217.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            15878..16921
                     /locus_tag="ACHBB7_000233"
     CDS             15878..16921
                     /locus_tag="ACHBB7_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9208218.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(17158..18039)
                     /locus_tag="ACHBB7_000234"
     CDS             complement(17158..18039)
                     /locus_tag="ACHBB7_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9208219.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            18198..18926
                     /locus_tag="ACHBB7_000235"
     CDS             18198..18926
                     /locus_tag="ACHBB7_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM9208220.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            19082..19399
                     /locus_tag="ACHBB7_000236"
     CDS             19082..19399
                     /locus_tag="ACHBB7_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9208221.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            19392..20000
                     /locus_tag="ACHBB7_000237"
     CDS             19392..20000
                     /locus_tag="ACHBB7_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM9208222.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            20055..20846
                     /locus_tag="ACHBB7_000238"
     CDS             20055..20846
                     /locus_tag="ACHBB7_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM9208223.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            20984..21472
                     /locus_tag="ACHBB7_000239"
     CDS             20984..21472
                     /locus_tag="ACHBB7_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM9208224.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            21523..22395
                     /locus_tag="ACHBB7_000240"
     CDS             21523..22395
                     /locus_tag="ACHBB7_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9208225.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            22571..22933
                     /locus_tag="ACHBB7_000241"
     CDS             22571..22933
                     /locus_tag="ACHBB7_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9208226.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            22942..23694
                     /locus_tag="ACHBB7_000242"
     CDS             22942..23694
                     /locus_tag="ACHBB7_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9208227.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            23708..25510
                     /locus_tag="ACHBB7_000243"
     CDS             23708..25510
                     /locus_tag="ACHBB7_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9208228.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            25787..26827
                     /locus_tag="ACHBB7_000244"
     CDS             25787..26827
                     /locus_tag="ACHBB7_000244"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9208229.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26879..29023)
                     /locus_tag="ACHBB7_000245"
     CDS             complement(26879..29023)
                     /locus_tag="ACHBB7_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9208230.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(29380..30753)
                     /locus_tag="ACHBB7_000246"
     CDS             complement(29380..30753)
                     /locus_tag="ACHBB7_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9208231.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(30753..31148)
                     /gene="rbsD"
                     /locus_tag="ACHBB7_000247"
     CDS             complement(30753..31148)
                     /gene="rbsD"
                     /locus_tag="ACHBB7_000247"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM9208232.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(31176..32093)
                     /gene="rbsK"
                     /locus_tag="ACHBB7_000248"
     CDS             complement(31176..32093)
                     /gene="rbsK"
                     /locus_tag="ACHBB7_000248"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM9208233.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(32201..33214)
                     /locus_tag="ACHBB7_000249"
     CDS             complement(32201..33214)
                     /locus_tag="ACHBB7_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM9208234.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(33295..33894)
                     /locus_tag="ACHBB7_000250"
     CDS             complement(33295..33894)
                     /locus_tag="ACHBB7_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM9208235.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            34080..34781
                     /locus_tag="ACHBB7_000251"
     CDS             34080..34781
                     /locus_tag="ACHBB7_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9208236.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            34791..37502
                     /locus_tag="ACHBB7_000252"
     CDS             34791..37502
                     /locus_tag="ACHBB7_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM9208237.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            37953..39581
                     /gene="treC"
                     /locus_tag="ACHBB7_000253"
     CDS             37953..39581
                     /gene="treC"
                     /locus_tag="ACHBB7_000253"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM9208238.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(39641..40822)
                     /locus_tag="ACHBB7_000254"
     CDS             complement(39641..40822)
                     /locus_tag="ACHBB7_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9208239.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            41074..42597
                     /locus_tag="ACHBB7_000255"
     CDS             41074..42597
                     /locus_tag="ACHBB7_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM9208240.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(42666..43394)
                     /locus_tag="ACHBB7_000256"
     CDS             complement(42666..43394)
                     /locus_tag="ACHBB7_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9208241.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(43407..44687)
                     /locus_tag="ACHBB7_000257"
     CDS             complement(43407..44687)
                     /locus_tag="ACHBB7_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM9208242.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(44828..45430)
                     /locus_tag="ACHBB7_000258"
     CDS             complement(44828..45430)
                     /locus_tag="ACHBB7_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM9208243.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            45675..45881
                     /locus_tag="ACHBB7_000259"
     CDS             45675..45881
                     /locus_tag="ACHBB7_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208244.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            46193..46912
                     /locus_tag="ACHBB7_000260"
     CDS             46193..46912
                     /locus_tag="ACHBB7_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9208245.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(46993..47676)
                     /locus_tag="ACHBB7_000261"
     CDS             complement(46993..47676)
                     /locus_tag="ACHBB7_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM9208246.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            47895..50081
                     /locus_tag="ACHBB7_000262"
     CDS             47895..50081
                     /locus_tag="ACHBB7_000262"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM9208247.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        16304 a         8891 c        10013 g        14948 t
ORIGIN      
        1 tgaatggtca aatacaagaa aaggtatttg gtcagtctcc aaaagtcata ttcttcatcg
       61 atcattaaca gacaaagaac tggcccgcca aggatatgag gacatctcct tgaagtacca
      121 gttcgtacac tcaacttatt gaaccgccgt atacgggtcc gtacgtacgg tggtgtgaga
      181 ggggcggaag gtcagttggc tttcccctct actcgatcgg atgtatttat cccttttgcc
      241 taagcacgta aaagcttgta tctatgataa ataaataaac atagcaaggt aaaccagcca
      301 atcaaagtaa gagaaagaat gaaatagaaa tagattggcg tttgataatg aagcgtgagc
      361 tcgttgattc ctttttgctg cttcacgatt ggggtcccga tatctgttac ttcataatca
      421 gtcaatgttt ttccattttg ctgtagcaca gtccgatcgt agacgataac agggatatga
      481 acagttttgt ccccagtgtt tttccacttg attgtcaaaa gaccgcctgg atgtgttttt
      541 ataaaagttg tttcgttttc tagtatttct tcgttatacc ggtcgtattt attgtctttt
      601 gtctccttat agattggcag atagtcaggt gtggtttttt ggaaaacgaa caagctggta
      661 gacatatctt tgctgaagaa tgttttcctt gcttcttccg gcgtatcgaa tagataagta
      721 tgtcgactga caaatgtttc attttcccac ttttccagat gctggtataa gttctggatc
      781 gtctgtgctg aaaaaataag aatgccaaat atacttaaaa gacggaacca cttttgttca
      841 gaccactggt tcagtgaaag ggcaaggtaa agtagaagca atagcgtaaa tggcacgaaa
      901 aatcgaaatg gaaactggat caaactaata aaaggcacat ttttttcgat caatgtcgtc
      961 catggaatca agctagtaga aagaaaaaag aaaacaagac ttgtgtacaa cacgattttt
     1021 tggaataagg tgttcttttg ttgaaagaaa ataaaaagca aaaaacctga taatatcaat
     1081 ataggaaagg ccttagggta atggagccaa taactgccat ccagatctat cgccttttga
     1141 ctcatttctc tgttaacaaa gggaggaaga atcttattcc cactgtaaac aatagctaat
     1201 cctgcccaaa tatttgctgt cagaacaaaa taaagcaaga ccgacttaat cagttgaagc
     1261 agtgcttttc ctttttcagt ctggttaaaa aagtaataaa gataaaaagg gatatacatc
     1321 aaagctaaca tgatcgaaga cagtaaatgg atctgtgtca taagtcccac tgagacagcg
     1381 actttgataa cagggaactt tttatgaagg acgaaatcag caatagggat catacataaa
     1441 ggataaaaac aagtccccca gctagtgaat ccttggttga tagaccagta ttgaatagaa
     1501 tacgtactca tgaataaaac ggctactggt acacttaccc aatttcgaac attgacatat
     1561 ctcagtaaaa tcagcataga aaatcctgaa ataagataaa gaatgaaatt tgaaagaatc
     1621 tgataatgaa accagctacc tgcaagcaac acgatcaatc cttgtaaata agcgaaaaat
     1681 gggccgtaaa cggcattcac gatcctgcca ctttgctgga atccataaag ggaaggaaaa
     1741 taatggaaat ccccatattt aatttgttgc gcggcatcat agaaccgatt gtaatggaat
     1801 ggggcatcag cacccaaaat tagtttatgt gtgaataaat gaggcatgag aaatgccaaa
     1861 gcaatgaaaa aaattgctaa aaatgggcca tattttttca attgtcgaac tcctttgcaa
     1921 actttagata aattcatcat atatcctatg cgagaggtag gcaagaaatt agaaaattca
     1981 tgaaaatatt tatgaatttt acaagaaaaa cataagtgaa agttctaagg agagaggatg
     2041 cgacataagt tttgttgcat ctccttgttt tgaaacagaa tttttcttaa tactcgaaac
     2101 aaagtagctt tttcatgacc tctcggtaaa cggtgttcaa tcagctgctt ctcggtaata
     2161 agctcactag cctagaaaat atgagaagct attttctgac tagcgctctt attactcaaa
     2221 gcataacgca gttttcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt
     2281 aataagccca acccaacaaa aaatatgagt agctattttt ttgggctgta cttattactt
     2341 gatgcaggac agctttttca caacctcatc ggctttcatt ttctattttt ttcctgaacc
     2401 catataataa aagaaagaac aaaaaaactt gcaagggagg acacatgtga agcagagtaa
     2461 gcaaaaacag tctcgtcgac ttttgtttcg tttgttttta ttaatatgta tcgtcgtagg
     2521 gttactgatc gctttatatc cattttatgt ggattcttta aatagtttta ttgatcaaaa
     2581 acgtatcgaa caagtacaaa aacggaccgc agctgaaatt gaagtgcagc gaaagaaaat
     2641 ggaggagaaa aaccaacgat taactgacca aggattcaat cctggtactg atcctttcaa
     2701 tgaaaagaac agaaatgaaa gtacaacatc cagccaatta gaggaatggc tgatcggaag
     2761 tatcaatata cctaagatcc aattaaatat ttcactttat gatcggctca atggtaggat
     2821 ccttgaaaat ggagcaggcg tcttgcaagg aacctctttc ccgcttggtg gaaattcgac
     2881 gcattcagtg atctctgctc acagcggttt acctaatcga cggttattta ccgaattaga
     2941 tcgtttagaa aacggagaca cgtttatttt gactgttttg ggggagaaat tggcttatca
     3001 agttaaaaat atacaagtgg tactgccaga tgacacgtct gttctaacga tttcagaagg
     3061 agaagactta gttaccttgc ttacatgtac gccgtatatg atcaacacgc atcgtttgct
     3121 agtgacagga catcggattc cttataacga atcggtaaaa aaagaagaag gaaaaggaaa
     3181 tcaggaaaga accatgcggc aatggtttat tcttgcagga acgataatag ctgtagtcat
     3241 actgttagtg tttatcggac gactaatcta tcagtataga ttaagtaaaa aagtgcttga
     3301 tttttcattt attattagtg attcgtcagg gaaaccggta aatggtagtt cattcatttt
     3361 gaaacataag aaaaaaacac ttactagaaa tggtgtgccg ttttcagtac aaagcgacca
     3421 tcatgggaaa gtcaagctgg atcaattgcc tggtggaact tatcgaatcg tttcagctga
     3481 tgatcctaaa gttgctgttt cttttggtat tagaaaagta aaacaagaga agatatactt
     3541 ttttgagggg cgaaaacttg taaaagagct tcaaaaaaat ggtttttatt ttaaaatcaa
     3601 tgaataagta aggagagaaa aaatgatccg cacaacaaca aaattgtctg ccaaagaact
     3661 ggaatctgtt ctatcgattt ggctgcatgc taacttggat tctcatgaat tcattccatc
     3721 aacttattgg gagaatcaag tagaatctgt taggaaagct ttaccagatg cagtgattta
     3781 cttagcagaa atagatcaag aaattattgg ttttgctggg gtgattgatc agtttatcgc
     3841 aggaatcttt gtgaaaaggg aataccggca acaaagaatc ggtacgcaac taatgaaaaa
     3901 aataaaagaa accaataaaa cactgacttt gacagtctat caaaaaaatc aatcagctat
     3961 tcatttctat ctttcacaag gttttacaat tcaaacacag caaaaagatg aagcagtaaa
     4021 tgaggaggaa cttgtgatga aatggaatca aaacgctgat ttttgatgaa cagaataacg
     4081 aaataatgct cccagcctaa ttttttgctg ggagcattat ttcattttga agtcactaat
     4141 ttaactgtgt gaatatgcag ttgttacaat ctttgataaa cggtgttcaa tcagctgacc
     4201 ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccatt tgtttctcta
     4261 atactcaggt caaaacgctg atttcacaac ctcttgataa acggtgttca aacggctgct
     4321 tctcggtaat aagcccacta gcctagaaaa tatgaaaagc tattttctaa ctagcgttct
     4381 tattactcaa agcataacgc tgatttcaca acctcttgat aaacggtgtt caatcagctg
     4441 accctcggta ttagctcaaa aaacggcaaa acatgaggaa ctatttttcc gtttctttct
     4501 ctaatactca ggtcagaacg ctgatttcac aacctctctt tcacaacctt attttactcg
     4561 tttataattt tcatcgaagt attttccgct acggatatca tctggcattc catcgtatgg
     4621 ttcttcgcta ataacatctt cattgtctat acctgcatca cccatataag tgatcgtagc
     4681 aaattcagga acaagtaatt ttggataagt ttcatatttt tcacgagctg cttccatttc
     4741 tgcgatatgt tcgttttgga aagtaacagt gatttctgga tgttcttcaa cccatttagg
     4801 gaaaaagatc attccgtgaa gtttcttttc atttggcata aagtccaatg ccacgtctgt
     4861 ccctgttggc tctgtccaag tgattttgaa tacgccgtcc gtcaatttaa cgatatcagc
     4921 ttcttggtct tttacccaac ggcctttgac cattccgcca tggatacggt aatcaacagt
     4981 atggtcattt ttggcatacc attcatattc ccaaccattg tcatacgtat agataaagtg
     5041 agttccaata aaatcttcca attctttaaa ttctttcatg tttaagcatc tccttataaa
     5101 ttaaaacatg taataaacta catgttttcg attactcatt tatgatagaa tggatttaaa
     5161 gatttgtcaa ggagggaaaa ctgatggcaa gaaaaaatac acttcgatat attttgttag
     5221 ggttattaag taagaagaag atgacaggat atgaattgaa tcaatcgttc aaaaatgaaa
     5281 taggagaatt ttggcaagca aaacatagtc agatttatcc tgaacttgca aaaatggaag
     5341 agcaaggaat cattcagcat caagtagaga tcacaggtga aaaattggaa aagaaagttt
     5401 atgaattaac agaagaaggg aaggaacaat tggcagagtg gattcataca cccaccaatg
     5461 aactaccggt taatcgagac gaatttgttc tgaaattgta tttcgtcaaa gacgtgaatg
     5521 atccggcatt agtagaaatt attcagcagc agcgtgacct tcatgaagaa aaacgacttc
     5581 atctattaga gcgaaaaaaa acgatctttc ctactgaaaa agaaaaacat gaaaattacg
     5641 gacactacct gattttagcg catgcgatca atcgggaaac agaatatgca aattggctaa
     5701 cagatgtatt agaagaaagt aaccgaaaaa aataaaaaaa tatccgaaca gtgatggaga
     5761 attcctttct atatgaaaga aacgcagatc accgttcgga tattcttatg ttcaaacagg
     5821 ccttttttca agtatttatg tagcatctac ttattaaaaa gcttggtact taggagatta
     5881 gtggcgttta gagttctttt aacaactgtt cgtagtcagc tttttcatcg acaggaagga
     5941 gcttgtaagt taattcgtct gtctcgttgt catgaatgat gtacatctta gcattctctt
     6001 ctttgaagtt accgatttgt aaaatcgtat agcgttcgtc gatcaattcg atatctttga
     6061 ttgcttttga aaaaacttta cggacatggt gcattttctt ttggcggatt tctgcctttt
     6121 ctgcagcttt tgacaaagta aatccgtcat ctaaatattt tttgatcagt tctactttta
     6181 tgactgtagg taaacgatat tttctaggac aattcgcatc agcttcgatc gaacgaatga
     6241 atcccttttg ttcccaatac cttaattgac gtggagacac acctacgata tcacttaatt
     6301 cactgatgcc aacaaccaat tgatcatttt ccaacaaacg tttaagggaa gccccaacca
     6361 tttttgtcac tccttttgtc ctcttgcttc ccttaattat atccgtatta acttatgtgt
     6421 caagtaggtt gacaaatagc gaatttagga ctaaagtatt ctagtagttt cagaaaaaat
     6481 tcagaaagaa ggagaaatat gagtaacaac caacccgtag atatttacgg aaaaccatat
     6541 aatcggacac ttttagttgt tgtcttatta attggtacat tttgtaccgt tttgaatcag
     6601 accttattga cgacagcttt tcctacattg atgaaagctt tcgatatttc agcgtctgat
     6661 gtgcaatggt taacgactgg tttcttgcta gtcaacggga tcatgatccc aataacagca
     6721 tggctgatca ataaattcag ttcaagaaat ttatatcttt ctgccatgac gatctttttg
     6781 atcggaacga ttacttgctt tacagcacct aattttagta cattactaat cggtcgtttg
     6841 atccaagctt gcggtgtggg agtatcgatg ccattattgc aaaatatcat gctatctatt
     6901 ttcccgccag aaaaaagagg atcagcaatg gggatgtcag ggatcgttat tggacttgct
     6961 cctgctttag gccctacatt atctggttat atcattgata actaccattg gcgtgacttg
     7021 tttggtatgg tactgccaat cgttgttctt gtcttggttt tagcattctt cctaatgaaa
     7081 agtgttattc cattgtcaaa tcctagcatt gatattttat ccgctatttt atcaacgatt
     7141 ggattcggta gtttacttta cggattttct agtgtaggag atgacggctg gggaagcttg
     7201 aaagtaattg gtttcttagt tgttggtgtg attttcatcg ccttgttctc atggcgtcaa
     7261 ttacatttgg aacatccatt cttggaactt cgtgtattca aatcatctac attcacgatt
     7321 gcagctgttt tagctggtgt aacaaatatg gcgatggtag gggcagaaat ggtcgtacca
     7381 ttgtatattc aaaatattcg tggagaatca gcattccatt ctggcttgat gttgttgccg
     7441 ggtgcattga tcatgggggc tatgatgcct gtgacgggtg cgattttcga taaatacggc
     7501 gcgaaacgtt tagccatcac agggatgttc attttgacag cggcaactgc accatttgct
     7561 ttcttgacaa aaacgactcc agttttatat atcgtgatcc tttatgcgat ccgtatgttt
     7621 gggatctcca tggtcatgat gccggtaacg acttctggta tgaatgcatt gccaaataac
     7681 ttgatcagtc atgggacagc ggtaaataat acattccgtc aaatcgcaag ctcgattggt
     7741 acagctattt tgatcagtgt gttgacgaat gtgacaaaag caaatatccc aagcaaatct
     7801 gtattggata atacgccatt agcttataaa gatcaggcaa tcaatgccac actgtcaggt
     7861 taccatgccg cgtttatcgt agcgatcatc tttgggattc ttggctttat tattgccttt
     7921 ttattgaaga aaaaagaacc tgttctaatg aaagcggggg ataatgcatg attattggag
     7981 ttttgattgt cagcttgctt gcttttgctt taacgaacgt gttcgctaaa aaaacatggc
     8041 aaaccttttt atctttgatt ttcggactaa tcttcgtcgc ttcactaagt ttgatcgtcg
     8101 ctaatttgtc caatcacttt ggaatggaaa aagtaacgga aacaaaaaca gaaaaaattg
     8161 tttccagtgc tgattcccaa ggagcagata tgcttttgta taaagcttta gggaatggaa
     8221 aagaaaaagt gtatctttac cgaacaaatg aaaaacaaga aaaaccaaaa gcaactggta
     8281 cggataacga aacaaataaa gtagaaaaga cagacggcga tgctgaaaaa gtgacgaaaa
     8341 ctacttattg ggaatacaaa aacgacatgt ataaattctg gttcaatatt gctgataaca
     8401 atcacgaata tgacagccga gtgaatacat tcaaaatccc agagacatgg gtagaattaa
     8461 gtacggacca agcagccaag ttagctgaat tagtgaagaa acagcaaagt acgatggaaa
     8521 gtgaagcaaa agcttatgtt caagacggta tggtaaaagc aatgactgaa aatccaaaaa
     8581 tgagcaaagc agagcaagaa caacgtacga aagccttagc tgctgaattc caacaacaag
     8641 cattcgcaaa attagtgaaa gaagcaaaag gcgaataata ttttgaacac catgtaggag
     8701 gttgtgaaaa agctgctctg catcaagcag taagaacaat cagataaaaa tagcactcca
     8761 tatttttcat ctgattgggc ttagtgccgt agatgcaagc ttttgaacac cgtttaagga
     8821 agaggttgtg agcctgccgt ttaactctga gtcacaagga gcaatttcaa aaaacagctt
     8881 ctcatgtttg ttgactattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttat
     8941 tcgaaaaaag gaactgcgac aagacttttg tcacagttcc ttcttttttt tgctatgcta
     9001 tactagatgt aaaaaatagg agaaataaaa tgagggaaat ttattctttt gaatcaaaaa
     9061 tctatgcttc cgaagtcggc aagggcgggg catatattat ttttccgtat gatataagaa
     9121 aagaattcgg tgctggaaga gttaaagtcc aagcaacttt tgacaatgtg ccgtatgaag
     9181 gcagtatcgt gaatatgggc gtgaaaaatc cagatggttc agtctgctac atccttggac
     9241 tacgaaaaga tattcgtaaa aacatcggaa aagaaatcgg tgatattgta gcagttacag
     9301 tgaagcaaaa ataaaagagg ttgtgacaaa agacgtgtca caacctcttg ataaacggtg
     9361 ttcaaaagcc agtttctcgg tagtaagtca aatttatgca aaaatgtgga aacccatgtt
     9421 tacaaaattt ttcttactac tcgaaacgaa gcggctttat cacaacctct tgataaacgg
     9481 cgttcaatca gctgtccctc ggtattagct caaaaacggc aaaatatgag aagctatttt
     9541 tccatttttt tctctcatac tcaggtcata acgctgattt cacaacctct tctttcataa
     9601 tggtttttca ccatctttat aataaactgg ttatttgata tggaaatcta ttttttccaa
     9661 ccaatctaaa atcttgtaac ctaactcttc tgacatcgga aaagaggcgt tgatttgtgc
     9721 attggagata tggtcaacat ggataccgga agtgatcgta caacttcccg gaacggcagt
     9781 tttgatgatc acagctattt tttcggctaa gacatcgtct ttatggaacc gaccatcgtg
     9841 gctagggaaa cgtacagttt gcatctctgt atcacgagaa aatgtagtga ctgttccgat
     9901 atgtggtgta tctccgcctg ttaaagtgat caataagtct ttaccgatta ctttaacagc
     9961 agcttggatc gtgaaaccta gtttggttac ttcaactatc gtttcttttt ccatattaat
    10021 caccctgcca acgttttgaa tgctcattta caatttcaaa ttgatccaaa agtttcattg
    10081 tatggtgatt aacaagatct tggatcgttt gaggctgatt ataaaaagca ggaattggtg
    10141 gtatgatttg cacacctagt cgtgctaatt tagtcaagtt ttccaagtga atggcattta
    10201 atggcgtttc acgaggaaca atgactaact ttcgttgctc cttcattgtt acatcagcag
    10261 cacgtgcaat caggttgtcg cctaaaccaa cagagatacc tgcgactgtt ttcatacttg
    10321 ccggtacgat gatcattcca tcggtcaaaa atgatccgct ggcgacagca gcacctaaat
    10381 ctttgtttga ataatggtaa tcaaacaggc tttcgaattc agttagtgat aagtcggatt
    10441 ccagctttaa gttctgtttt gcccatggac tgatgattcc gtgtgtttct atcatcggat
    10501 attcttttag tttcttcact aggttgatgg cataaatggt gcctgaagcg ccacttacac
    10561 caatcacgat ttttttcttc cgttgtgcca attgttccat tttccgcact cctttttatg
    10621 atgtcaaaac agttgcagat cattcattat ttcaaatatt tttcccaatc ttctacttct
    10681 tggaattttg cacgtacaaa atgttctttc atatcgaaag gaactgtacc gtcaaagata
    10741 gtttttgcac ttatcccacg gaaacgaata gagccaggat tatattctgg taattctgat
    10801 gggtccaatg gatggttcct cattcctggg atcacgatga tatctttatc tccttggaaa
    10861 cgagtgttga aagtccaaac tacatcgttc atgtcgaaaa tatcaacatc ttcatcgact
    10921 aaaatgactg tcttcaattc tttgaaggct gataaagcaa gaatagctgc ttgacgctga
    10981 ataccttcat ccgcttcgtt ttctttacgg atttgcatga tggtcatcaa tttaccacca
    11041 cctgcaggag gattgtagac atttactact tttcctggaa tagctttatc gaccaaggca
    11101 agaatacttg cttctgtagg aataccggcc atagatacgt gttcttcact gggaccaatc
    11161 gttgtttgca tgattggatt gtcttttcgg tgagtaactg ctttgacttt cactacttgt
    11221 acagctggat tagcatcccc agtatagcca ggaaattctg gcattgcttt tcctgagttc
    11281 gtgttgatat cttcttgcat tgtttcattt ggcatgattt ctgcttcaat gatgaattca
    11341 gcacgagcaa tacccacttc atcgactgct ataccatcaa cgatttgaat aggttcgtta
    11401 cgcaaagctc ctgcacacca tagttcattg tagccaagtg gagtggtagg tggttcgaaa
    11461 gtcgtgccaa tggcaattgc tggatctaaa ccaatattga tagtgatagg cattggttta
    11521 ttcaaggctt cgaattgttt ttggaagtga ccaatgtggc gtccgccagg catgatgtac
    11581 ataccgatcg tatctttatc ttctagaacc atacgatgga tcgttacatc gctcattgtt
    11641 ttttctggat ctgatcctaa gactacaccc attgtgatgt atggaccagc atcttcagga
    11701 gtatttgtag gtgcaggcaa aatgttgcga atatcgaagt ctgcatcggt agctagatga
    11761 acgacttctt gtgctggagc gtctgctttg tctactttca caggtttgat tggattttct
    11821 acagcgtctt tcaagaaacg accaagtgtt cgataatcat ggtgcaaaat ttcacctaca
    11881 cgtttacgac tagccattgt tccgatgttc acacgtgtgc cggggaaacc tttgatattg
    11941 ttgaaagtca tagctggtcc ttcttgagtc ggacgtttta ctgtaccgcc agcaccgata
    12001 taacggtaaa caccagataa ttctgcttca ggatcgattt caatatctgt ttcgtgatat
    12061 tgacctggaa gtgtttttag ttcttcgatg acttttctta aatcatacgg ttgatctgtc
    12121 atgagagatt cctccttagt tgtctttacc tatctatagt aaaaggtgaa aaagaggaaa
    12181 acaattcaac ttgttcggta aagtattcta caatggaatg gatgcgtttt tttgataata
    12241 gaataaacta tgatatactt atcactaaaa gaaagcgcat acttagtgtt ggaggaagga
    12301 aagtgaaaaa aatggaggag tcatttccta aagcagtaaa agtggaaaat atagcgaata
    12361 ttttgaaagt tacatttgaa aacggagaag taaaatacgt gaaatctcat tggacagaag
    12421 agataaccga tgccttgcaa tttggaaaaa aaggaagagg aaaaagaaaa aatcttctcg
    12481 cactttctcg taatatgtgg attgggactg aagtaacgat tgaagcagat ggaacagtat
    12541 tcataaatgg aaaagacagg tatacgccag aagagttgtg gtacaaagga aagaaaagta
    12601 taccagaatt ataaaaaata attaaaaaaa acagcgcgaa acaaggaaca tattacttcc
    12661 tcgtttcttg ctgttttcta ctatttcagc gctcttatga ctcaaatcta aatgtggttg
    12721 tcacaacctt attctgcacg caaagcgata gcggcgtctt ttttcgctgc cattcgtgca
    12781 ggaagatgac cgccaagcat cgtcaatata gtagagacga tcactagaat aatcgcatgg
    12841 acaggattta gttgagcaac attttttaga tccgtcatgt tgtataagat gctattgata
    12901 gggaacgtag ctagccatgc aataaacaca cctaatatcc cagaagaaat acctaaaata
    12961 cacgtttctg cgtcaaatac gcgagtgatg tcttttttac gtgcacctaa agcttttagt
    13021 acaccgattt cttttgtccg ttcaatcaca gatgtataag taatgatact gatcatgatc
    13081 atacttgtta ctaaagagat ccctgcaaaa gcaataagga cataagtgat agcatccatc
    13141 aatccgccag tcagttcagt catcgttcca gctaaatcag tataaatgat tttatcttca
    13201 tctgatttgc ctttattgta atcatctaga taatccaaga tcttttcttt gtcttcaaaa
    13261 ttgttcggat aaatcatgat acttgaaggc aagctgtctc cacctaaata actaagcaat
    13321 gtttgtttgg cattttcgtc aactttttca gtagttaaga catttacacc actgtctctt
    13381 tgagctttga cgatttctga ttctttgttt tcgttgacga tttgagtcgt taattgatcg
    13441 ctataagcga ttcctggaga taagagattc attgtagaag aagatttcac gcgtaggatc
    13501 cccgagatct tcaattcatc agaagcattc tgatacatcg catcataatc tgtattaggg
    13561 ataaaattcc cagttggaag ttttgtatag taagtattgt tgtttactaa tttgaatgtt
    13621 gtaccaacga tatcatcaaa atcaagtttt tcgtcttctt tcacatcaaa tccaagattc
    13681 ttcaatgcgt tgatattagt attattattg ccgtctacga tcagcactac atctgttgct
    13741 gaggctggat aacttccggc aagtaaagaa taattgtctt ttaaaaagtt ttctttgcta
    13801 gtgtcaagct gtgtagggaa acttgacact cccacacctg tcattgctga catcgtacta
    13861 gataaagaaa gagattccgt atcaggattt tgattcgaga agctgacagg ctgtactttg
    13921 ccatttacat cacgtaaaag attgattcct gtagtacggg taaatccgat attattactt
    13981 aagttaggat cgatgtctgt tacgtaatcg acgtatgttt gatcaatctt attcgtgtgc
    14041 tgtgctttat cttcttcgct gacttttgca gtaatcgttt tgctgtctgg ataatccgct
    14101 ttgcttgcac ccaatccggc atcgtcacga gtctggttag tcgttacctt agaaatagta
    14161 atcggaaatt tcgccatcgt ttcagcttgc gtgttatcga tttgtttttg gaatccagaa
    14221 gaaagggaca gaacgatcgc gattccgatg atcccaatac tggaagcaaa ggaggtcaag
    14281 aaagtacgtc ctttttttgt tcggatattg ttgaaggaaa gctttaacgc tgtccaaaaa
    14341 ctcatctttg ttctgcgtaa gttaaattga tcatcttttg gtcgttcgat gtgaggatgg
    14401 gaatctgtta agatcttacc atcagaaaat tcaatgattc gatctgcgta ttgatgggcc
    14461 agttcaggat tatgtgtgac catgatcact aatttttctt tcgataactc ttggatcaat
    14521 tccatgatct ggatacttgt ttctgtatct aacgctccgg ttggttcatc gcacaaaagg
    14581 atatctgggt cattggctag tgctcgagcg atggccactc gctgcatttg tccaccagat
    14641 aactgatttg gctttttatg catatgattt gtcaagccga ctcggtgcaa tgcatcttct
    14701 gcccgttttc gtttttcgtc tttagaaaca ccacttaatg tcatcccaag ttcaacgttt
    14761 tcaataatgc ctagatgacc aatcaagttg taactttgaa aaatgaaacc gatagagttg
    14821 tttcggtagg catcccagtc actgtcctta aagtctttag tagactttcc gttgattacc
    14881 atatcaccgg aatcataatt atctagtcca ccaatcacat tcagcatagt cgttttacca
    14941 gaaccactag gacctaaaat cgctacgaat tctttttgtc tgaaagcgac agacacacca
    15001 tccaatgctt tggtggtggt ttcgccgact ttataatatt tttttatatc ttttaattga
    15061 agcataatca cccttctccc gtattgaaat attcggtata ctaataagta tatcagaaca
    15121 atgatcgtag tatacgaaat aaatgttaac tgaagatgaa ctacaagaaa aaatctagaa
    15181 atttttcaaa aaacagaggg agatcaatga atcgtatttt agtagtagaa gatgatccgc
    15241 aattacagtt gctttaccgt tctgtgttgg aacgagcggg cttttcagtc atcaccgcat
    15301 tgaatggaga ggatgcgctg aaaaaactag aagcgacacg tatagaatta atcattacag
    15361 atatcatgat gccgaaaatg gatggctatg acttgctaga ctcattacgt agcaccagat
    15421 ttgacacacc tgttctaatg attacggcaa aggccgattt tgaggataag aaacgcgggt
    15481 tcaaattagg agcagatgat tatatgacaa aacccatcga tgtcaatgag atgatcctac
    15541 gtgtggaagc attgcttcga cgttcaaaga tcaattacag cagtagcttg ataatcggac
    15601 aaacaaaatt agagctagat gcttatacag tgttacaaga tcagcattcg ttgattctgc
    15661 ctcaaaaaga atttctgctt ctgtataaat tactgtctta tcccaacaag atcttcactc
    15721 gccaacagtt gatggatgat atttggggat tagatacgaa tacagaagaa cgaacgatcg
    15781 atgtacatat caaacgttta aggacccgtt ttgaaaagac ggaagatttt caaatcatca
    15841 cagtacgggg actaggctat aaggcggtta tttaaagatg aaaaaaaagc tattttcaca
    15901 attatggatc tattttgcta gtattgtctt catttcgatc ttagtgacac tgctgtgttt
    15961 tctcggtttt attttcttat tgtctcgaca ttctatctcg cctgctgaat cggctggaaa
    16021 accaacgtta tacccgttat tcgtctttgc ggggttcagt atggtcgtgg gtacaggaat
    16081 atcgattttt gttgggcggc gtatcctgca tccaatcagt gcgctaggga caaatatgag
    16141 tctggtagca acgggagatt tttcgattcg aatggatgaa cagcagaaag tagcagaagt
    16201 acagcaactg tataaagatt tcaacgttat ggtccaagaa ctcaatagta tcgagacact
    16261 gaggaatgac tttgtgtcca gtgtatccca tgaattcaaa acaccactag caacaatcca
    16321 aggatatgtt caattgttac aagccccaaa tctctctgac gaagaacgcc aaatattttt
    16381 atatcggatt atcgaaagta tcacgcagct ttcacaatta acagagaaca tcttaaaatt
    16441 gaacaaactg gaaaaccagc gtatccaact ggaaaagaaa gagtatcgat tagatgaaca
    16501 aatcagagaa gtaatcgttt tttttcagcc taaatgggag aaagaacagc tagaattgga
    16561 tatcgaactt gctgcagtaa attacacagg caatgaagag tttttgtacc aagtgtggct
    16621 gaatatcatg gataacgcca taaagtacaa tcaaatcaat ggacagattc atatcaaact
    16681 atttgagaca gccacagaga ttgtactgga agtgaccgat tcaggtgtag gaatgaatga
    16741 agagaccaga gatcgaatgt ttgaaaaatt ttatcaagga gatacaagtc gtcaaatttc
    16801 tgggaatggg ctaggacttt cactggttaa aaaaattcta gaactgcatg atggacgtat
    16861 tgattatagc agtatcgaag gagtaggaac gacagcaatg atacggttaa gtaaacaata
    16921 ggaaaaagaa taaaacccct aaaaattcta ttgaaggttt ttaggggttt ttagtcgttt
    16981 ttttgtgatt agggaaatgt tcttacggtt cttttggcat aaaaatcgct gctttcttat
    17041 tgaactacga gaaatgttag aggtgtcatt gaaacaagga catgatgcaa gaaagagagc
    17101 gaaagtcatg cgtcgctctc tttttttggg tggatagatg gacagaaaat gtagggctta
    17161 gttttcttct tgatttaaat actcccaaaa tgtatctgaa gccaaagaat gctcaccgac
    17221 tttacgagta aatgcaagat aactctctgt tgttggagaa atagggagaa tcacagtatt
    17281 ttcttttaat agattcttga ctgttttttc cataacaata gcaatcccca tgtcatttgc
    17341 aatcatattg atgatcaagt cgattcttgc tcctttataa gagatttttg gttcaaatcc
    17401 agcttcgcga cacagatcta gtacaggttg atacaagttc gtagtggggc caaggagcaa
    17461 aaagttctcc tttttcagtt cgcttagatc caatttcttt ttactagcta aaggatgatt
    17521 tttaggtaga actgctacaa aatcgtctgt ttcagtaggc aaccactcaa tcgaacttgg
    17581 tggggcctgg aaacttctag caaagtggat cgtgtcacta tgttcattgg cgtcattgta
    17641 agttaattcg acactttcca cttctttcaa ctggactgta aattcaggat ggcgtttcaa
    17701 aaactcggtg attttggaaa aacctttata attgatcatt gtcggtatcg ttaagatatt
    17761 taatgaaaga ttgttgtctt tcgtataggt ttcaagcact tcttgcatag catggtattg
    17821 ttctacgaca ttttttacat atgggagcaa taagcttcca gcttcggtca attcgatttt
    17881 tctatgggcg cgttcgaata atttgacgtc taattctgtt tctaacgaaa gaatctgttt
    17941 agaaatattt ccttgggtgg tgaattgtcg atcagcagtt ttggtgaaat tcattgtttc
    18001 ggctaaatca agaaaaactt cgaatttatg gatatccatc atattcctcc tacttattcc
    18061 aataaagaat actttaacac aaaaaatgaa ttggtgtaat cttctctgtc tgttacaatc
    18121 tttcttgcag cgaaagcaat aattacagaa atttttctga tttttttcgc ttcgtagaaa
    18181 aagagaggag ttttttgatg ccaactggag ttttgatcaa tgtaggttcc gttttgtttg
    18241 gcggactaat tggcggatta gtaggaaata aattaagtga acattttaaa gcacagctga
    18301 cgatggtttt tggtgtgtgt tcgatgggga tgggaatcta ttccattgcg ccgatgaaaa
    18361 atatgccggc tgttattttt gctttagtga taggaacagc gattggtttg attgttcatt
    18421 taggtaatgg gataaataag ggggctgcgc tgatgcaagt acctatttct aaaatcttcc
    18481 ctagtgaaaa gcttggtatg acccacgatg aatttatcag cacattagtg acggtcattg
    18541 ttctattttg tgcaagtggt acggggatct atggaagttt agattctggg atgacaggag
    18601 acagtacgat tttgatttct aaatctgtcc ttgatttttt cacagcagct atttttgcgt
    18661 gtaatttagg gtatgtcgtt tctgtgatag caattcccca attcattatt ttttatattt
    18721 tgttcttatt ggcaaaattt atttatccat tgactacacc agacatgatc ttagatttta
    18781 aagcttgcgg cggattcttg atggtcgcta ccggttttag aatgatcaac gtgaaaatgt
    18841 tcccagtagc tgacatgata cctgcaatga tcgttatcat gccactgagc tggatgtgga
    18901 caaattggat tatgccactg ttataataaa aatcaaagag aagccatttt ttgctgacta
    18961 aaaaggatca aatcttttag tcagtttttt tgtatttgac aaactgattt tgaaactata
    19021 ttatatgttg tataatatag attgaaataa aatattacac ttgagaatag aaggtgacgt
    19081 aatgcacatc cagattccaa ctgttcttct tgatggtact gtgctagcag cattaagcaa
    19141 ggaagatatg tatgggtatg ccttaacaaa aatcgtacaa gaaagtctct ctgtcagtga
    19201 atctacacta tatccggttt tgagaagatt gaaaaaagaa ggatatcttg agacctatga
    19261 tgaacctttt gaaggcagaa accgtcgata ttactcgttg acaccaatgg gaagtcgtca
    19321 tttagaagaa attattgatg aatggattgt ttttcgagat tctttagacc ggttattgga
    19381 ggagagcaca aatgaatgaa tatttgaatg aagttgtgga tcatcttcaa gaaattcctg
    19441 aagacgatcg ttttgatttg atccagtatt atgaggaata ttttttagat tctggaaaaa
    19501 cattagatca gatcattgat gaatatggta caccaaagca gtttgcctta aaattaaaaa
    19561 tcagttactt ttcagaagaa gaacaagcag aaaacgagtt agtgaaaaat ccaaaaagac
    19621 aaattcgcct gatttggtta atcattatcg gtttatttgc ttctcctgtc ctcattccac
    19681 tagcactggg gtttatattg gttattgggg gactattggt tgctttacta gcgctcattt
    19741 ttagtatcta tgttttatgt atcagtatct taggtgttgg cctattcatg atcatctcag
    19801 gaatcagtgt gtttggacaa tcgattctga gtggattatt ctttatcggt gtcggtctgt
    19861 tcgccacagg aagcgcagta tttatttcgc cactgttgct gaaaggaaca aaagggcttt
    19921 tttatgtcat gatgaacttt gttaaatgga tgggacgacg ttttattaca aaaagagatc
    19981 tgcattcagc gggcgtttga aggaggacac ataaattgag attgaaacat tttttgatta
    20041 ccggtattgg tctgatgctt actggtggcc tcctagcagg tgcaacttat acattaggcg
    20101 ctcaaaagag tctgacttgg gataatggtc ctcggttgat cgatatggtt actgagacaa
    20161 aaaaaatcaa ggatgcaaac aaaatcgtca tccatgcaga aaatcaaaca gtttcggtca
    20221 tacgcggatt ggattttgag ataaaaacga cttatgataa gacagaaaag ccagaaataa
    20281 aggagaaaaa tggcgtgttg actgtgaatg cagtcaaaaa atcaacaggt gcaatcatta
    20341 gtttagaaga cacaagtgca caaatcacta ttgttgtccc tgcagatcat gaattatctg
    20401 aattgacaat cgaagggaat aatagtgcaa ttgatatcaa cgaagtatca atcaaaaaag
    20461 tgaatactaa gacacatgat ggctgggtat cgttcaatga atttacagga ggaacaataa
    20521 ctgcgaaaga tgaattaggc tcacttcgat tagatgaggc caatgtggat aacttgatcg
    20581 tcacaggtga aaacagcaac ttccatctag aagacagttt gatcagcaaa gaaggaaaag
    20641 tcatcctaaa cggtggcaat ttatctttgc atgagatgga aaacagtggc tatactatcc
    20701 aaacgtctgg tgaatcttat attgaaaaag actaccagcc aataacaaat actttgttag
    20761 aacaaggaca gaaaaagcta aagatcactg ctaaagacac atcgatcaat attacgacgg
    20821 atcaagagga caatgaaaca gattgatcct gtactcaact ggatatttta agaaaaaaca
    20881 gcaaagatcc ttccaatctt tgctgttttt catcgcttaa cgcacataac tatagtaaaa
    20941 taagaaagaa aatatctaaa aagaaaacag ggtgaaagag tatatgaagg tagaagaagg
    21001 tcaaagagaa aagttaaaaa cagaaatgga aagattacat acttatatca ctcaactaag
    21061 ccaaaccttc tatgatccag acaaagaaaa agtgatggtt aattacccga acaattcaga
    21121 aggtcgccaa ttagagcaag tgtaccatga agttttcaaa catttattga cggtgaagaa
    21181 agagctggat tattattctt taccgattat cgatacagga atattgaagt atgatgggaa
    21241 gaaggaacga tttatcttta aatctgttcg ggaagatttg cctctttcag cagggatgga
    21301 tctagaggtc ttggtagaag actattttac agaagaaaag cactgggttc gaacaaaatt
    21361 ggattattta ccgcaggcag ccggagggac acaggcaagc ggttggtaca tcactgaaga
    21421 caaagagtta gaactagaag gtgtaatggc gcgtattcga aaaaaaaatt aaaaaagtaa
    21481 cattagatat ttattgaagg ataaaggagc gcaggaagta gcatgagaga aaaaagagca
    21541 ttttatatca atggttatgc agggattgtc ggattggtcg tattagcttt agttggattg
    21601 tttttcttct atttaggtat gtggcaagcg aaagtttggg cacttttttt aagtattttt
    21661 ctttggttga tcacgttatt gttgttaagt tcggcaactg tagtcagtcc taatcaagca
    21721 aaagtaattt tgttttttgg acaatattta ggtacgattc gagaaaatgg ctttttcctt
    21781 acgatacctt tggcacaaaa aatgactgtt tcgttgaaag ttcggaattt caatagttct
    21841 gttttaaaag tcaatgattt ggatggaaac cccatcgaaa tctcagcagt agtagtcttt
    21901 aaagtcgttg atacagcaaa ggcattattc gatgtagcgt attatcaaga ttttgtagaa
    21961 atccaaagtg agacagccat tcggcatatc gctagccaat acccgtatga tacattcaat
    22021 gagaatgatt tgacgcttcg aggaaacacg acggcagtgt ctgacgagtt gcaaaaagag
    22081 ctgcaagagc gtttagcagt agcaggtgtc gaagtaatcg aaacacgctt gaaccattta
    22141 gcctatgcaa cagaaatcgc tagtgccatg ctccaacgtc aacaagcaaa agccattttg
    22201 tctgctcgac aaaccattgt tgaaggagct gtcacaatca ctcaaatggc cttagagcaa
    22261 atcgaagatg gacaagaaat caatttcacc gatgatcgga aagtccaatt gatcaataat
    22321 ttacttgtct caatcattac agacaaagga acgcaacctg ttatcaatac aggcgacatg
    22381 aaagaccgta agtgacatac tttttgtaag ataggaagaa ggagtttagg acggattatt
    22441 gtcttgaact ctttttttag ttgttttaaa gaaaatgaca aaattgaaag aagaatgata
    22501 gttgattcga aggcagaatt gttcgctttt ttctataatc agtatataaa gtaagagaga
    22561 aggggaaaaa gtgaaaaaac ttctgatcgg tctacttgtt ttcatcgttg tggtacttgc
    22621 aggggtgaaa atcgcagatt acgtagttat gggtggagaa gaatactatg tacaaattac
    22681 gacagatggt acaaaaacag tttcaaaatc agactctgga caagaaacag tcaactatag
    22741 atatatcctg cctggctata cgaaagaggg taaggaaaaa gaactggact tcaagggaca
    22801 attgactcga ccgcttcgta aaggagcata tttaaaaatc acctggaaca aaaataaagg
    22861 tgtcacttcg tatgaagaag tcgaaaaaaa agacgtgccg aaaacagcac tggaaaaact
    22921 aattaaaggg tagggaaaga aatgaacaaa atagtcgaag tacagaatgt aacaaaagta
    22981 tacggaaaaa acaatgagaa aaagacgcaa gcattaagcg gcatcagctt cgatgtagaa
    23041 aaaggcgaat ttattgggat catgggcgct tctggttctg gtaagtcgac acttttgaat
    23101 attttatcca cattagataa accaacagat gggcatatcc gaatcaatca aaaagatgtg
    23161 accacattaa aaggaaatca actagcagat tttcgtgcga atgagattgg ctttattttc
    23221 caagatttca atttattaga aagcttgaca gcacaagaaa atatcgctgt tcctctttct
    23281 ttacaaggcg tcagaccaaa agaaatcaag caacgtgtcc aaaaaattgc agaacgttta
    23341 tccatctctc atattttgga aagctatcct tctgagattt ccggtggaca aaaacaacgg
    23401 gtcgccgccg cacgtgcatt gatcacacaa ccaacgattc tattagggga tgagcctaca
    23461 ggtgcgctgg attccaaaag tgcccgggat cttttagata cgatggacga attgaataca
    23521 aaagatcatg tatcgattct tcttgtgacg catgatccgt tttctgcaag ttattgccaa
    23581 cgtattttat tcatcaagga tggcggtatc catcaagaag tgaaacgtgg agatcaaagc
    23641 cgtgattcct tctatcgaga aatactatct attcttggaa acttggaaca gtagggggag
    23701 attgactatg ctttggaaat tatcacttac tgggatcaag agccgtttaa gagactactt
    23761 ggtgttattc tcaggattag ttatggcatc tgctattttt tacatgtttg aatcaatggc
    23821 aagcaacgaa gcttttttga aaagtaatac gatcgttgcg gctacagtta tcattttccg
    23881 attcggttct gttttactag ggatcatcac attcgtttat attttatatg caaattcctt
    23941 tttgatgacg atgcggcaaa aagactatgc aatgtttatg atgctgggag caaaaggcag
    24001 aaaaatcgca cagatgattt tttctgaaac attcttagta gggatcgcag ctactttagt
    24061 gggttcagct gtcggtgtag gattgacaag tatcgtccac cgattattag tggatcagtt
    24121 agatctcaat atttcgcatt ttacaccttt tagtattcac gggttgttga tcactgtttt
    24181 gttttttgct gttttgtttt tattagctgc tgttgtcaat gctttttcga ttgtcaaaaa
    24241 accgatttta gcattgattc gtgcggatca gacaccaaca agaatcaagc agcataaatt
    24301 cttgtttttc cttgaagtag tactgggaat tgcttttcta ggctgcggtt actatatgat
    24361 gaaacacgtg gggacctatc aagtctttgg tataggggta gccttagtta cgatcgtatt
    24421 aggaacttat ttcattttcc attctgtgat cattttcttt ttatcattac taaagaaatc
    24481 agaacagatt tcattgaaaa aattgaacaa tttcacattg tcacagttaa actttcgtat
    24541 tcgtgaatat acacaaatgc tctcaatggt cgctatgctg tttgccttgg cactgggcgc
    24601 tttgacggta ggattaggtt ttaaaaatga aattgcagac ttgacagaaa acaattctgg
    24661 atatgatcta gtgatgaata atcctcgggc agaagatcag caaaaaatca aagaactttc
    24721 accaacactt tctgcttctt atactcaaaa agaagatgcg ggaacgattt actataatgc
    24781 aagtgaattt gcggctcaac cattgatcat gataaaacat gaagatggaa caccgccgaa
    24841 gatcacgaaa gaaaaagttc ctgctaaaaa aatgaatgaa ctgactgtac aagaggaact
    24901 tcgaagctat gaattgccgg aacagcaaga aaaagaaatc aaggtagtca gtcaatcaga
    24961 atttgatcaa ctgaacttgc cacaatccac tttacagcta gttcaggtaa aagacttcca
    25021 agcaaacctg aaaccaatcg aaacacttgt tgctcaaaat aaaatgaaca atccatcttt
    25081 gcaaaaagta gagtacagca accagaaata tgaaatgtat gaagtgtata atggtctatt
    25141 ctctggtttt gaatttatgg gattcttcct aggtttagct ttcttgacca tgcttgctag
    25201 ctgtttgatg ttcaagatct tgtcaggaag caagagtgac attgctcgtt atgaaatgct
    25261 tgaaaagata ggaacacgac gcgggttgct taaacaatcg atccgtagag aagtaggcgt
    25321 gttattctta gcaccaggaa tcttaggtgt agtccatgtc ttattcggat tacaaatgtt
    25381 tggtctattc atgcaaaatc catacaaaga tatttgggtt ccgtttacga tattcttcgt
    25441 attgtatttc atctactatg tactgacgac atggttatat actggaatcg tattgaaaaa
    25501 gagattgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagctcc
    25561 tcatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttgt
    25621 tcggaaaaag aggttgtgac aatatttttg tcacaacctt tttttcacgg tcttttatta
    25681 gaatatagta aattttttct cgttactcac ggaataagct gttaagaaaa cggtttccat
    25741 gtatactgga atcagtgcaa atgaaagaaa agagggtgca aggaacatgg aaatcaatga
    25801 acaaaatttt ggcgaaaact atcacttgat tacgatcacg aatcaaaatg ggacatcttt
    25861 gtctgtcagt gatcttgggg cacgggttgt tcattttcaa acaataatcg ataatgaaaa
    25921 acgagagttg atcttaggtt ttgatgaagc aaaagaatat ctagaaaaag acccttatat
    25981 cggtgcttca atcggacgta tggctggacg gattacgaat ggcacattca aattagatga
    26041 tgaaacgttc gaagtaaaaa cagacccgga cactggtcat tgtttacatg gtggtgcacc
    26101 tagctttgaa gccaagcagt ggcattatac agtcatcaat ggtgaaaacg aagcatcggt
    26161 cattttctat cagactagcc cagatatgga aaacggattt cctggaactt tagatgtaga
    26221 agtacggtat acgctgacga acgatgatac atggcgagtg acggtccaag ggatcagtga
    26281 ccgcaaaaca gtattcaatc ctacaaatca tgtctatttc aatttaaccg gtgatccagc
    26341 acagtcaatc gacgaacata tactttggtt gaatagccga ttatttgcta cgctcaataa
    26401 agatacaacg cctacaggag aagtagtttc agtaagtggc acagcatttg acttccaacg
    26461 tcctaaaaaa ttaggtgagg tctttgccag tgattttcgc caatcaatga tagtgggcgg
    26521 catcgatcat ccatttttgt tgaaaaaaca atctggttta ctaaatgctg cggaattgac
    26581 aagcccagat gaaaaagtat cgatccaagt aaaaacagat gcttcaggaa tcgtgatttt
    26641 tacagcaaat tttggagaag atggaccgga aatgcgcggg aataaattag taaatcatgg
    26701 cggtatcaca tttgagactc aagaactgcc aggagcggag cgacttgaaa gttttggaga
    26761 tgtcactctg gaacctaacc aagtacgcga gtttgtaacg gaatacaaaa taaatataaa
    26821 gaaatagaat aaattaaaaa gggagtgtga cagtgtcaca tccccttttt aatttattct
    26881 atttctttaa aggcagatca agccagaatg atttgtctaa acttaacaag gttagtacag
    26941 gaacagttgc agctttaact gtacgagtag ttgtttctgg tatctgtctt gccgtaaaat
    27001 tgttccacaa atcccagatt gaagcgacac ggaagatgga agaagcgttg ttgacgcgtt
    27061 ttatcgttga aaattctaat cctgtgattg gcacgccatc tgttgtccat ttgattggca
    27121 catcgaatcg cttagacata ttgaggattt cagcgtttgc ttcttcagtt gttgctgctt
    27181 cttttttcgg ttcatcatat tttgtcaggt cgtacgcttc gatgatcgcg ttcaattttt
    27241 gtgaatattg gcgatctgta gcataacgtc cttccaaata tttggttgct tctttgtagt
    27301 cttttgtttc cgacttccaa gttggtttgt agaaatcagg attgccagtc agtccatttt
    27361 tgatcaagtc ggcatagtct tccaaacttt ccttgtaaga aggatattta cggaattgtg
    27421 cgcggatttg gtagctttgt ccggaactat cttgttcaag tgtattgaag gtcacgctgc
    27481 ttcctttata tgatcctttg ataccaaaaa ggttatgatt tggttcgctt gataataagc
    27541 tgtttccaga tccactttca aggatagctt gagccatcat gactgatgcg tagattcctt
    27601 ctttttgccc gatttcctgt gcatcatctg caatcttttt caagaactga tcagttgttt
    27661 ggttttgact gaaatggaac gagccatcta atccatctaa aatagatgag ccgcctgtca
    27721 gcgaacctga agcagcaggt aaacctgcta agtttactcg gacagcaaaa tcaggcgtga
    27781 aatctgaaat agaagttact cgaactttat ctcctggttg aggcgcatgg ataaattgtc
    27841 cgttcccaat atagatacct acatggtgag taacaccgga agaaggtccc cagaagacga
    27901 ggtcgccagg ttgggcctga cttactggaa tctgcgtacc tgcatattgc tgttcgcctg
    27961 tccagccacc gatataatga ccggttgctt tcatatatac gtaataagtc aaaccagaac
    28021 agtcaaaagt atttggacct tttgctcccc atacatatgg ttttccaaga tgcttgaatg
    28081 cttctcgaac gattgcttgt tggacattcg ttgtatttgt aggcaataca gcatcaattg
    28141 gtgattttac aacaaaatca gaagaagcag aagatggatt ctctataccg ctttgatttg
    28201 ctgtatttgg acgagatgaa gcattgttcg ttccgccatt cgatgaacca ttgtttggat
    28261 ggtttggttc tctctgagta tttccattgt tattcgatcc agatgggttt gtttgattcg
    28321 aagaattccc gctgttcgaa ttattgttat tattgtttga attatttgaa ccattattat
    28381 tgttgttatt acccacatct ggtttgtttg gcgtttccgg atctggtgta ctagggactt
    28441 cttcactagg atcagttggt ttctctgttg aagtatccgg tccagcttta aattctgcag
    28501 taatagataa attgttcgta cctactgtaa aggtctttgc atcataattt acttctttgg
    28561 caccattaac agcgactgaa gcaatttgtt caccttcagc tggcgtaata gtgtaagtta
    28621 ccttcgtttc cgctaataaa ccttctaccg ttccactttc cgtaatatcc aaaggttgct
    28681 gttcagggga atccgctgtc gaagcagtca gttggatctt accaatcaaa gaaggagcaa
    28741 ttgtaaggga atatgtctcg gctggctttt cattttctga tggatcagcc ggatcaattt
    28801 cagttggctc ggtcgatcct gttgttgagt tagttgttcc agttgggtct tctgtaaaag
    28861 aatccgttgt ttcactagta gctgagctgg atgtagtcgt atctgttgtg actgtttgat
    28921 cagctatttg attatctacg gcaaatacgg tattggtcga caaaacagac ggagcaatca
    28981 gccaagtagc agctagcaaa cgtatcgttt ttttattttt cataataaac tagtcctttt
    29041 cctttcattg attttctgtc ttattcagca ttcattagac attttttgga acagataatc
    29101 gtgtcgaaaa gctttttctt ttcataaatc tgtctgtgta ttctaaataa aacaatttgt
    29161 aaataagcaa cgagcattca ttcgtttttc tttgacgttc attttattgc atagaccacc
    29221 tttgtttggg gataaacaag tggggataaa aatggttatg catatgtaaa ttataccaaa
    29281 gtttggaaag tatcacaaaa atttataaaa ataatgatcc taaaaagaaa aagactgaaa
    29341 tatgtgataa acatctcata cttcagtctt tttcattaac tattcataag acattaggtc
    29401 ttcatcaaac ccgctaacga gagtagtgat cattgctacg actagactaa cagccagtcc
    29461 tgctaagtac cagaaaaaag taccggaaat gaatgcaggc aatgtggtca gactaccaaa
    29521 tacaaaagcg atcgctttca cttgggcaaa tcccattaca gctccaccag cagctgcacc
    29581 aatgcaagca gcaacaaatg gttttttcaa tgggatcgca attccgtaaa cagttggttc
    29641 ggtcactccc gcaagtaaaa tcgaagcaaa agcagaaccg gagatcgaac gggtttttga
    29701 acgtttactt cgcagaaata cacctaaagc tgcaccagcc attccaaaat tggctgctcc
    29761 tgtcaacgga acgattcggt cgaacccata agcagagata ttactgatca tgacagggtt
    29821 tacagcccat tggataccaa atattaccaa aatattccaa ccaccagcga tgattgcgcc
    29881 actgatcggt ccgttaatat ccatcatcca agtaattgcg gcagctaaac cttcgccaag
    29941 gtaaacaccc gcaggcccga ataccattaa cgtcaaagga accgtgataa ctaatgaaag
    30001 taaaggtaca aatactaatt gcatattttc gcgaatatat tttttgagga atttttcaac
    30061 aaaagaataa aagaaaatac ctaaaatcgc cggcatgaca gtagacgtat actgcatcag
    30121 tactacagga atccccagaa aatcagtagc tgctttgcca ctgtctgaaa gtaaagaggt
    30181 cagattcggt tcaatcagtg ctgcagcaat gacagccgca atatatccat tcactttaaa
    30241 cgttttggca gcagaaaaag caagaaagat tggcagaaaa taaaagacgg cattcgacgc
    30301 agcagacaaa atatgataag ttccgcttgt ttcactaagc cagccaatcg tagtcgctaa
    30361 aacaaccaga ccttttaata taccagaacc ggcaaacagc ggaatgatcg gcgtaaagat
    30421 ttccgatatc aaaccaaata gtttttcgat cggatttagc ttttctttcg gagctgaatg
    30481 atcgatttga cgcgtcaatt ccttgtaagc aggagccaat ccctgatcca tgattatttc
    30541 tgcatatcta tcgagtaaaa tgaaatactt aatggaaggg atttgctgtt ttaatttttc
    30601 tgcattggct actggctgtt ttttcaattt gatacgaagc cgagtcgcac atggaaggac
    30661 agaatcaata ttgtcttttc ctcccaactg ttcaatcagc atatgaatct gctcggacag
    30721 taccatctgt tcaggattca ttgtcgtttc cattagaaga ccactccact cactaatata
    30781 atattggcgt aaggtgtcat ttcgcctgta cgaataaatg catgacagtt attcaactct
    30841 tttttcattt cttcatgtgg gataaagcta atcggtgtat ctggtaacaa taatttgatt
    30901 ttttccaata tttccgggtt cattgtcttt atttcttccg ccaattcaat cgattgtacc
    30961 tcaagttctt ctaaaacgtt ttctaaaaca gataaaaaac ttggaagacc gtttgagaca
    31021 gccaaatcta ttttttcagt accaaagggg acaggcatgc ctgcatcccc gatacttaat
    31081 ttatcaaagt gccccatttg tgcgattact cgtgaaatat ctgaattgat tacttttcct
    31141 tttttcatgt ttgtttcttc ctctcattgt taattttaga ctgctgattg ttctaaagct
    31201 tcgcggtaag ggatagaagg ttgcgcacca taagactgca cagcaacaga agaggctaga
    31261 tttccataaa ggatcgcttc tttcaagtta ctaaggtctg gctgaagtgt gctgcttaat
    31321 gcgccaataa atgtatcacc tgcggctgtt gtgtcaacgg catccacttt aaaagcaggg
    31381 acgataccgt gttctttttc tgtatgatag aaagcgccgg cactgcctag tgtaatgatt
    31441 actgttccaa tacctaattc atggagtttt tcggctgctg cgaccaaact gttatgatct
    31501 gtcactctga ttcctgtaat gatttctgtt tctgtttcat ttggtataat gatatctgtc
    31561 ttttcaagta gttcagtagg aatcgtttca cgagctggtg caggattcag gatcgttgtt
    31621 tttcctgcgt cttttgcaat agaaaaagct aggatcgtcg catctaagct actttcaaac
    31681 tgagcaataa taaagtcact gtttgtaatc agttgcttgt tttttaggac ttcttgttca
    31741 tggaaagcat tatttgcccc tgcatggatc aagatactat tttctcctgc tgcgtctacc
    31801 ataatcattg cttgtccggt tgtggcgcct tggatttcag ctatcccaga tgtatcgatg
    31861 ttttcttttg tcagtagatc taataactgt tgtccttcgc tgtcagcccc aacgcctcca
    31921 ataaaagatg tccgtgcgcc tgaacgtttg gctgccactg cttggttggc acctttacct
    31981 ccgccagcgt gaaaaatctc atgcgcatgc atcgtttcgc ctggcttggg catgttggtt
    32041 aaacgaaggg tagtgtccat attaatactt ccaattactg taaccgtgtt cattttacaa
    32101 acctccaact atctcattta ttttgatggg aaaattattt tttaaaacgt tttagtaaaa
    32161 cgttttaata gttcgataaa aatatagcac accttgaaag ctatttcaag ggtgcagttg
    32221 aaaatctttt ttctaatcgt acaggtaatc ttttttcttc ccattctttt tctgggaatt
    32281 ggatccgatc taacaataac ttggcagatg tttgtcccaa ttcgaagatt ggttgtgcga
    32341 tcgttgtcag tttcggcttg atatactcgc acatatctat attatcatag ccaataatgc
    32401 tgtagtcttc agggatgctt tttccagctt cctctaatcc gcgatataat ccaaaagcta
    32461 attcatcatt taaagcaaac acaccagttg cagcagaatc aagaagttct gctgtgatct
    32521 ggtagccacc ttgttttgag aactgtgtag gtaagaggat cagttggtcg tgagggattt
    32581 ggtaaacatc aagagcagat ttaaacccct caatccgagc gtggacattt tccggtggat
    32641 tttcaggata gacaagggcg atcgtttgat gtcctagact aagcaagtgc atgcccgcta
    32701 ggtaaccacc gcgaaaatcg tctgttcgta cggcatcact aaatccttca gattttttct
    32761 gatccaaaac aatgaagggc cggccttgtt ttttcaaatt ttcgttgatt gcatctgtag
    32821 agacagcaga agtcgcgata atgaatccat ccacacctcg acgtgtgagc tcggctagat
    32881 attcgatttc tttttgatga tcagaatcag cattacataa tatcgtgacg aaattctgct
    32941 tatacaagat atcctcaatt cctcgcatca gtgtactgaa gaatggattc gtgatatcag
    33001 gaacgagtac accaattgtt ttcgtttcct tggtaatcat ttgtctagca aaatagtcag
    33061 gctgatagcc taattcttct ttggcagcta aaactttctg aacagttttc ggactgaatt
    33121 tctccgtatt tccatttaat atcaaggaga cagttgtgat tgaaacgccg gaaagtttag
    33181 cgacttcttt gattgtgatc ttttttttca ccatgttcat actcctatct aaatcctgtg
    33241 gttattttcc catgaaatca aaaagaagta aatgaaaatc acgtgtgcca gtacttattt
    33301 gctttgttgg atagcttcga ttatttgttt cgttgagcgt actcgtgcag ttttcggaaa
    33361 tatctttttt atgaacaatt gatgggattc tttgtcacga tcactgcaag catcttcagc
    33421 aagcacgata tggtacccgt gttgaaaggc atctaatgca gtagcatata caccgtttgt
    33481 tgtcgctaca ccgcttaaaa tgatagtttc gattccacgc cggcgaagtt gaagatcaag
    33541 agaagtacca aaaaaagcac ttggattgta ttttgtgata gtcaacatat tatctgtatc
    33601 tttcaaagaa tccttcaaaa gtaaatcagt gaattctgga ggtacagaag cagtcattcc
    33661 acccatatca gtttgttgac tcaaataagg gaaagtggag atgtctacgt tcactaaaac
    33721 tttcaaggca gcggtacgtt tcaatgttct tactaacaaa tcattgtttg tcagaataga
    33781 tgctgcagtg tgtggagaga gtgtaccgtt atttacgatc tctttttgca tgtcgatcgc
    33841 gacaaaagca gtttttgata aatcaaaaat catattttcc tttaaatcat tcatgatgtt
    33901 ccttctttca aataacaatg ttttttatgt aagtaaatca taaatcgttt atttgtcaac
    33961 taaattaaag gattcctttc tttagtctag agaaattcag tggtaaaata aataagtgtg
    34021 agaaaacgca ttcgatgcat agatatagcg agaaatatcg tgaaaatgga ggaatcagag
    34081 tgaagtatgt cgaagtaaaa aatgaataca aacgatacaa aatgggagaa acgaccatta
    34141 cagcaaatga cgatatttct ttttcaattg aaaaaggcga attagtgatt atacttggtc
    34201 caagtggagc aggaaagtca acgattctta atattttagg tgggatggat acacctgatg
    34261 aaggtcagat cattattgat gacacagata ttgcgcaatt ttcagataaa cagcttactg
    34321 cttaccgacg aacagatgta gggttcgttt tccaattcta caatctcgtt cctaatctaa
    34381 cagcaaaaga aaatgtggaa ttggcaacgg aagtttctcc tgatgcgctt gatcctgtag
    34441 aggtattgag gcaagtgggc ctagctcatc gattgaataa ctttccgtcc caattatcag
    34501 gaggcgaaca gcagagggtt tcaattgctc gtgcactagc taaaaatccc aaacttcttt
    34561 tatgtgatga accgacaggc gcgctggatt ttgaaacggg caagcaagta ttgaaattat
    34621 tgcaaaacgc tagcagacaa cacggcaata ccgttttgat cattacacat aattctgctt
    34681 tagcgcccat cgctgataga gtcattcaca ttaatgatgc taaagtaagg tctgttgaat
    34741 taaatgatca tccttcatcc attgatgaga ttgtatggta gggggatttt atgaaaacaa
    34801 gaacatatct taaagcaggt tttcgcgaaa ttcggcaatc taaaggacga ttcattgcaa
    34861 tcatccttat tgtcatgcta ggaacacttt tatttgtagg tgtcaaaact gctggacctg
    34921 tcatgcaaaa aacgatggat cactatgttg gtacagcagg actttctgat ttgcaaatcg
    34981 tttctactgg tggcttgaca caaaaagata ttgctcaagc agaaaagatc ccagatgcaa
    35041 aagtagaaac aggaaaacag atctattatt ccaatactgg caaaaacgaa gtgattcaaa
    35101 tattttctta taacaaaaat aacaagcaga acaagctaca attaactgat ggaaaactcc
    35161 cggaaaaacc aaaccagctt gtattggatg aaaaagcgga agatgaaggg tataaaatcg
    35221 gtgatattta tcagattgat tcagatgaat tgaaagaaaa agagtatacc atcacgggct
    35281 ttgtccgttc tccattgttc atcaacaatt tggagagagg atacgcgaac gtcggaaatg
    35341 gtagcgtcga ttattttgtc tatttacctg aatccgtttt taaaacagat atttattctg
    35401 tcttatatct tgattttaca aacgtaaaga atttgaatac gtacagcaaa gcttacaaag
    35461 ataaaatgga gcaaaatcaa gaaaaggcag aaaagtattt acaagatcga cccgaagaac
    35521 gcttagaaga gctgaaaaaa tcggccaacg aagaattaga accggcacag caaaaaatag
    35581 cggatggcaa agcacaaaca acgcaagcaa gaacacggtt agaagatgct aaaaaacagt
    35641 tagcgcaaca gtcagcaact atccagcagc ttcctgaagc gcaacgaaca atcgctgttc
    35701 aaacattatc tcaacaagaa gctcaacttg ctgaacaaga aaggcaatta gttgagaaag
    35761 aacgtgaact ggcctctgca gaggaagaat tgaatcgaaa tgcagaaaaa attgaaaatt
    35821 taactaaacc atcataccta tacaatgaac gttcagaaaa tgtcggtttt caagaatttg
    35881 gtgatctgtc tgatcgtatc gctgcgattg ccaatgtttt tcctgttttc tttttcttta
    35941 ttgccgcatt gatcaccttt acgacaatga cacgtatggt agaagaaaac cgcagggaaa
    36001 tcgggacatt aaaggcgtta ggttatacga aagttgaaat cgcaaaaaaa tatgcaatct
    36061 atgcaagtct agcctcaggg ataggtattg ttttaggaac gatattagga acgaatcttt
    36121 tgccaaggat catttttgag ctgtctaacg agcggtatga tattgggagt gcgcttattt
    36181 tttatgactg gccgccgatt ttacaagcag ctgccgcatt cttcattgcc gcatttggcg
    36241 ctgcaatgat cgttttattc aaagatttaa gagaacggcc tgctgcattg cttcaaccga
    36301 aagcaccgaa gccagggaaa cgcatttttc ttgaatacat cactccgctg tggtcacgcc
    36361 ttagctttaa tcaaaaaatc agttaccgaa atctcttccg atataagtca agaatgctta
    36421 tggcgattat cggaattgct ggatgtgccg ggctgatggt tgcaggcgtg ggattgaaag
    36481 attctctaag ttctgtttca gccaaacaat ttggaccgat cattgactat caagcaatcg
    36541 ttacattgga ttcatccacg gaagatagtc gtcaaaaagc agcggatatg ctgcagaagc
    36601 aagaaaaagt aaatgagatc ctagctattc atactcaaac cgtagaattg agaaaaaaag
    36661 gacatgcttc gcaaagtctg acagtgatcg taccaagtaa aacagctgat ttggatcgtt
    36721 ttatccattt aactgatctt gaagagaacc cacgctcctt gcctaaaaca ggaatcgcta
    36781 ttacgcaaaa agtagcagaa ctatttgact tatctacagg agatcatatt tcgctttatg
    36841 atgaagatca aaaagaatgg aaagtcgaag taaaagaaat cgtgaaaaac tatctaggta
    36901 gctttattta tatgtcccct gaatattatg cttcagttac tgatcaatcg atgatggata
    36961 atgcattttt gctgcgtaca gataagatga caacaaaaga agaagaaatg ctttccgaga
    37021 aactgcttgc cacagacgct gtgacaaata cgtctttcat gtccaaacaa atcgaaacgc
    37081 aagaagaatc aatggcgaac ttagactcgg ttgtcttaat ctttgtcgta ttatcaggac
    37141 tgctggcttt tatcgtcttg tataatctaa ctaatatcaa tatttccgag cgtgtacgcg
    37201 aattatctac aataaaagtc cttggatttt ttgataaaga agtcacgatg tacattgttc
    37261 gagaaaatat catctttact cttttaggaa tcatcggagg atttggtgta ggatatatat
    37321 tgactgactt catcttgcaa caagcgtcta tggagaatgt catctttcca ttagtgatca
    37381 catggtgggc ttatgtctta tcagcaggat taactatcgt atttacggtt attgtgatga
    37441 ttgttaccca tttcaaatta aagcatatcg atatgattga tgcgttgaaa tcgaatgaat
    37501 aaaaacagag gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggcaaaa
    37561 tagtttttca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacacca
    37621 tgaatcagag gttgtaaaaa agccgtcctg catcgagcaa aaagaatact caaataaaat
    37681 tagctcctca tgttttacat ttgagaagac tgattgccgt agataaaagc ttttgaacac
    37741 catttataga agaaacaaga atattttgct attttaaaca agaggataga caaatacatg
    37801 tctgatctct tgttttttat tgggcaacag acctcttaga atgtataaag ataaatttta
    37861 caaacgcttt cggttcagtt ataataataa atgataactt gtatatataa gttgtggtag
    37921 gtaataaatc agttgaatgg aggtaaatat ccatgacatt taaagacaaa gtcatttacc
    37981 aaatctatcc caaatcttac aaagacagta atggtgatgg aattggagat ttaaaaggaa
    38041 tcaaagaaaa actcccgtat ctgaatgatc taggcatcga catgatttgg ctaaacccta
    38101 tttatccaag ccctcaaaaa gacaacggtt atgatatctc tgattacaca gccatcgatc
    38161 ctatctttgg tacgatggag gaattcgaac agctaatagc cgaagcaaag aaataccgga
    38221 tagaaatcat gctcgatatg gtgctgaatc acgtttcgac agaacatgaa tggttcaaaa
    38281 aagcactagc aggggaccag cattatcagg acttcttcat tttaagagaa gagccaacgg
    38341 attgggtatc aaaatttggt ggaagtgctt ggtcgccatt tggagatacg gggaaatatt
    38401 atttgcactt atatgatcgg acacaagctg acttgaactg gcggaaccaa gcagtaagag
    38461 aggaactatt caaagttgtt cgtttttgga tggaaaaagg ggtaaaagga ttccgtttcg
    38521 atgttatcaa tgttatcggt aaagacgaac aattgaaaaa taataaagaa aatgatggca
    38581 aacctgaata tacggacaaa ccaatcaccc atacgtatct aaaagaattg aaccaagcaa
    38641 cgtttgggac ggattccact atcatcacag tcggtgaaat gagctctacg acgatcgaaa
    38701 attgtatctt gtatacagaa cctagtagag aagaattatc aatggtcttt aatttccatc
    38761 atttgaaagt cgattatgaa aatgggaata aatggtctac tcctccattt gattttgcag
    38821 aattaaagcg actatttcat acatggggag aaaagatgag tgagggtaac ggctggaatg
    38881 ctctattttt taataatcat gatcagccac gtgcgctcaa tcgatttgta gctatcgatc
    38941 agtatcgaaa acaaggagca gagatgctag caaccatgat ccatttgaac cgtgggacgc
    39001 cctatattta tatgggagaa gaaatcggca tgatcgaccc agactattca tcgatttccg
    39061 actatattga cgtagaaagt ttgaatgctt ataagttact ccttgaacaa ggattcacag
    39121 aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga taactctcga acacccatgc
    39181 aatggtcaga cacagaacaa gcaggattta ctacagggaa accttggtta aaagttggag
    39241 gcaagctaga agagattaat gtagaaaaag aactatcttc tgaagattct attttgtcct
    39301 attacaagaa attgattcgg ctgagaaaaa catatccaat cgttgcacaa ggagattatc
    39361 atgcttatga agcaaatcat ccggaagttt atggctttct tcgccaattt gaaggacagc
    39421 agctgcttgt attaacaaat ttctatgata aagatacaag gatcgccatc ccagaaggat
    39481 tcacagaagc caatattctt atcgataatt attccgatac tgttcttcat caaagtctgg
    39541 agctaaaacc ataccaggca attgctttgc tgaaaaatta aaacaaaaac acaaaacgtc
    39601 gttcctacga gctattctcg taagatcgac gttttgtgtt ttatttaaaa taatcttctt
    39661 cagaaaatga agagttttca tactctcctt tatcgccata aaatttattg tagcgttgtt
    39721 tgaatttacg gaaaataaaa gtaacgagta atttgactaa cgtatagata ggaataccaa
    39781 aaataacgcc cacaattccc attagatcac ccatgacaag taaaacgatt aagatcgtaa
    39841 tcggatggat ctgtaaacga tcacccatta ctcgaggaat caccaagtca ccgtggagaa
    39901 gttgtacggc aaaccagaca atgacaaatt tgacagccat aaaagtagag tcttggaatg
    39961 caatgatcaa acctggaaag aaggcgataa acggcccaat atatggaatg atacataaca
    40021 acccagcaga aatagctaaa acactagcaa attctaatcc gatcagtaag aaacaaaccc
    40081 agtagactgc tccaaggata aatgaagcaa tgatttgccc ctttagaaat gaacccaatt
    40141 gttgattagc aatctttgtc aaatttttaa aatctgtacg aaatgctggt gggacaatcg
    40201 ctaaaactga ctgataaaat ttttgaggat ctctcaaaag gaaaaaagcg acaattggac
    40261 cagtaaataa agtaataaaa atagctgtaa cagttgaaaa aatgttgctg atccgttgtg
    40321 cgccactttc ccaataattt ccaataaatc cagctatatc agacgtcacg tcgtcaatag
    40381 aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt ctccaaaaat tgtcggagac
    40441 tctcttgaaa atcttgaaac aaatctggca aactactgat caatgtcttt gcctgttctt
    40501 caagaaaagg aataagccat atgcttccaa gccccaataa aaataaaata atcacataaa
    40561 cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa acgtttgatg actggttcca
    40621 aaagataata caagatcagt gcaaacaata atgggggcaa gatagtagcg ataatgattc
    40681 cgacaggctt gaatataaac gagatctgat ggaaaaaata aatgatcagc gcaagtaaga
    40741 taactattcc taaaacatag taagaggttt ttcctcctaa aaagcggatc aaacgattcg
    40801 ttgtatctgt ttcttttttc actctttttc ctcctttatc tataacttca ttatagacaa
    40861 aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa aatatcgtca atcattcata
    40921 taaactttct catcaattaa aataagaaaa cgcttgacag ttcttttaac catatgataa
    40981 attaaatcct gttgctaaaa cgtaactatt acggtttgcg aaaagcgaat cggaacattc
    41041 aaatgaaaga agatgagaag aggagtgtca taaatgaaac gattgatagg tatattggcc
    41101 atgctggttt tagcaggtat gttaactgct tgcggggcaa gtgggaaagc cgaggacagt
    41161 aaagaaaaat tgagcgtgat gacgaccttt tatcccatgt atgattttac aaaagcaatc
    41221 gtcggggatg aaggagaagt agagttattg attccagcag ggactgattc ccacgattat
    41281 gaaccttctg ctaaagacat ggcaaagatc caagatacag atatctttgt ttataatgat
    41341 gaaaacatgg aaacatgggt ccccgcaatc caaaaaacat tacaagaagg caatgtccat
    41401 acgataaaag cgacagaagg tatgctttta cttccgggaa gtgaagaagg acatgatcat
    41461 gaccatgaac acggggaaga aggccataca catgagctag acccacatgt atggctagct
    41521 ccaagtttag caatcaagca ggtagcaaac atacgtgatc agctgattga agcttaccca
    41581 gaaaaacagg aagtatggac aaaaaatgca gcagcctata ccgagaaact tcaagcactc
    41641 catcagcttt atcaagaaac ctttaagcaa gcaaaacaaa gatcttttgt cacgcaacat
    41701 gctgcattca actatctagc gttagaatat ggattgaatc aagtatcgat tgctggtctt
    41761 tcttctagtg aagaaccgtc tgcggcgaga attgctgaac tgaaacactt tgttaaagaa
    41821 catggaatca actatattta ttttgaagaa aacgccaaag atagtattgc cagaacgtta
    41881 gcaaacgaag caggtgtatc tttagaagta ttgaatcctt tggaaggatt gacgaatgaa
    41941 caaatagaaa atggagaaaa ctatctttct atcatggaag caaacctcga agcgttgaaa
    42001 aaaacgacag agacagaaaa cccgctagaa gatactttga cccccaaaaa agaaaagagc
    42061 gtctacaacg gttattttga agacgaggat gtacaagatc gctctctttc agattgggca
    42121 ggaacttggc aaacagtaga tacgtatgta gaagatggga cttttgatcc tgtttttgaa
    42181 tataaagaaa aattgaatca ggataagaca gcacaagaat ataaagaata ttatacgaag
    42241 ggctatcaaa cagatattgc ttcaatcaag atcagtgaga aagagatggc atttacgttt
    42301 aagaatggaa caacgaaacg ttcaaattat cgttatgtag ggaaagaaat cttgacctac
    42361 gaggcaggaa accgtggcgt aaggtatcta ttcgaagcag aagataagga aagtggcgct
    42421 tttagatata ttcaattcag tgatcacacg attgcaccgg ctgactccga acattttcat
    42481 ttatatgcag gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca
    42541 ttttatccag atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag
    42601 gcaaaaaaga accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt
    42661 tctttttatg aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt
    42721 gccaacgtga cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca
    42781 taggatttat gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca
    42841 tagaattgta attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa
    42901 gtagaaaaat attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt
    42961 ttgaagatat ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata
    43021 tgctcgacat tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg
    43081 atgttgcttt ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt
    43141 cctaaaggat ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct
    43201 tctagttcga tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg
    43261 agaagttctt ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg
    43321 attctatttt tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata
    43381 taaatgggaa tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa
    43441 cgctggtttt caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc
    43501 aagataaaca agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt
    43561 ttcaactggt ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa
    43621 tgattcttct tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca
    43681 atcgcattgt ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc
    43741 agttcgattg gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg
    43801 agttttaatg acagtaaact acggatctca ggaactttca tattatccaa catacttaga
    43861 ttggccggaa aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac
    43921 cgtaatcctt ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg
    43981 taatcatgtt tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc
    44041 tgttgcatcc tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc
    44101 caataataaa ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc
    44161 aaagctaaga catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat
    44221 gtaaataata tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa
    44281 gtctctaacg atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca
    44341 atgtgaaaat actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa
    44401 tcgccgatgc ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg
    44461 taaaccaatt gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata
    44521 gcaattagag cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa
    44581 atcagaatga ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata
    44641 caaattgtgt aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc
    44701 cattgatttt taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat
    44761 tttgaattca gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt
    44821 taattgttta atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg
    44881 taataggaag caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt
    44941 aagaagctga gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct
    45001 ttaataatgg gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat
    45061 agcgaagcag taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag
    45121 tcaattgcag atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca
    45181 gacaaacgaa ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag
    45241 aaagaaaggt gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga
    45301 ccgctagttt tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca
    45361 ccatatcttc ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt
    45421 tatctttcat ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt
    45481 cttttgtttc ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct
    45541 tcgttttcca agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat
    45601 gacaagtttg tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg
    45661 atggagggat tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta
    45721 aatctgattt atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg
    45781 tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact
    45841 ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag
    45901 agcataaaga ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa
    45961 acagcttttc atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg
    46021 tttatttgaa aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg
    46081 agttactttt ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt
    46141 tatttctata aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat
    46201 aattgtgatc attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct
    46261 tgcccaagat ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc
    46321 gctattttat tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa
    46381 acgctttttg gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat
    46441 ctacgaagat agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga
    46501 agtaaagcag tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc
    46561 acaaaagaaa ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag
    46621 aatcaaaaaa agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta
    46681 ctataaagaa ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa
    46741 aatagtgatc gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat
    46801 cgtacaagaa atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat
    46861 ccgctacact acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa
    46921 ctaagcaaat tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt
    46981 gattcattga gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt
    47041 ctgagtgagg gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa
    47101 cagatgatga cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag
    47161 atagtggaaa aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt
    47221 cgttgctacc ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat
    47281 tttatcaaaa gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc
    47341 gggatgctca tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag
    47401 aacaaacaag cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt
    47461 tagaagaatc cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca
    47521 ttctccgtca aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa
    47581 agcaccatat tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag
    47641 tcctattgta cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata
    47701 tttttgtctt ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac
    47761 taaaatcata tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat
    47821 agacaacgaa actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga
    47881 aggaaatcaa tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct
    47941 gggagaacaa gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta
    48001 cctatgaagc ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag
    48061 aaagtgaagg aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca
    48121 atcaagaaca aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag
    48181 gagctgggag cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa
    48241 aacaagtaga cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac
    48301 taaaaaaacg agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc
    48361 atagtatctt ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc
    48421 aaggaataca aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac
    48481 agctgatacg taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt
    48541 ctcggttaga ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta
    48601 aagaaaaata tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg
    48661 gattgctcac ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg
    48721 tactccaaaa tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa
    48781 aatatgatta tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg
    48841 tgaaacggat gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga
    48901 gtatctatgg attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc
    48961 ctaccaaaac tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag
    49021 caaatagatt gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac
    49081 aaaaagaagc aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt
    49141 ggctaatcaa acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca
    49201 tattataccg atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata
    49261 ttccttttgt actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc
    49321 aacatttcta tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc
    49381 aggcctacca gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat
    49441 gcgatcaata cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga
    49501 taagccccaa tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta
    49561 aaacagacca gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta
    49621 tagctaagga aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg
    49681 ctactttcag aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa
    49741 aaacccgctt gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc
    49801 acaaaagtaa aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt
    49861 tgccttataa cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac
    49921 cagtaacgac gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa
    49981 atcgtctgta tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat
    50041 ttatcaaaga aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca
    50101 gaagtggtca gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaattt
//
LOCUS       DAYYKN010000006        45410 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000006 DAYYKN010000000
VERSION     DAYYKN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.006"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..1307
                     /gene="tgt"
                     /locus_tag="ACHBB7_000263"
     CDS             162..1307
                     /gene="tgt"
                     /locus_tag="ACHBB7_000263"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM9208248.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1404..1784
                     /gene="yajC"
                     /locus_tag="ACHBB7_000264"
     CDS             1404..1784
                     /gene="yajC"
                     /locus_tag="ACHBB7_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM9208249.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            2089..4686
                     /gene="adhE"
                     /locus_tag="ACHBB7_000265"
                     /gene_synonym="adhC"
     CDS             2089..4686
                     /gene="adhE"
                     /locus_tag="ACHBB7_000265"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM9208250.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            4915..6021
                     /locus_tag="ACHBB7_000266"
     CDS             4915..6021
                     /locus_tag="ACHBB7_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9208251.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            6038..7066
                     /locus_tag="ACHBB7_000267"
     CDS             6038..7066
                     /locus_tag="ACHBB7_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM9208252.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            7072..7551
                     /locus_tag="ACHBB7_000268"
     CDS             7072..7551
                     /locus_tag="ACHBB7_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM9208253.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            7567..8862
                     /locus_tag="ACHBB7_000269"
     CDS             7567..8862
                     /locus_tag="ACHBB7_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM9208254.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            8873..9697
                     /locus_tag="ACHBB7_000270"
     CDS             8873..9697
                     /locus_tag="ACHBB7_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9208255.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            9862..10761
                     /locus_tag="ACHBB7_000271"
     CDS             9862..10761
                     /locus_tag="ACHBB7_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9208256.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(10887..11756)
                     /locus_tag="ACHBB7_000272"
     CDS             complement(10887..11756)
                     /locus_tag="ACHBB7_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9208257.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            11909..13369
                     /gene="rhaB"
                     /locus_tag="ACHBB7_000273"
     CDS             11909..13369
                     /gene="rhaB"
                     /locus_tag="ACHBB7_000273"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM9208258.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            13366..14622
                     /gene="rhaA"
                     /locus_tag="ACHBB7_000274"
     CDS             13366..14622
                     /gene="rhaA"
                     /locus_tag="ACHBB7_000274"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM9208259.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            14636..15481
                     /gene="rhaD"
                     /locus_tag="ACHBB7_000275"
     CDS             14636..15481
                     /gene="rhaD"
                     /locus_tag="ACHBB7_000275"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM9208260.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            15483..15794
                     /gene="rhaM"
                     /locus_tag="ACHBB7_000276"
     CDS             15483..15794
                     /gene="rhaM"
                     /locus_tag="ACHBB7_000276"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM9208261.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            15876..17027
                     /gene="fucO"
                     /locus_tag="ACHBB7_000277"
     CDS             15876..17027
                     /gene="fucO"
                     /locus_tag="ACHBB7_000277"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM9208262.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            17154..17912
                     /locus_tag="ACHBB7_000278"
     CDS             17154..17912
                     /locus_tag="ACHBB7_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM9208263.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            18012..18656
                     /locus_tag="ACHBB7_000279"
     CDS             18012..18656
                     /locus_tag="ACHBB7_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM9208264.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            18663..19670
                     /locus_tag="ACHBB7_000280"
     CDS             18663..19670
                     /locus_tag="ACHBB7_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM9208265.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            19686..20528
                     /gene="kduI"
                     /locus_tag="ACHBB7_000281"
     CDS             19686..20528
                     /gene="kduI"
                     /locus_tag="ACHBB7_000281"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM9208266.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            20548..21351
                     /locus_tag="ACHBB7_000282"
     CDS             20548..21351
                     /locus_tag="ACHBB7_000282"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM9208267.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(21600..22664)
                     /gene="fni"
                     /locus_tag="ACHBB7_000283"
     CDS             complement(21600..22664)
                     /gene="fni"
                     /locus_tag="ACHBB7_000283"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM9208268.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(22661..23746)
                     /locus_tag="ACHBB7_000284"
     CDS             complement(22661..23746)
                     /locus_tag="ACHBB7_000284"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM9208269.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(23759..24736)
                     /gene="mvaD"
                     /locus_tag="ACHBB7_000285"
     CDS             complement(23759..24736)
                     /gene="mvaD"
                     /locus_tag="ACHBB7_000285"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM9208270.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(24729..25673)
                     /gene="mvk"
                     /locus_tag="ACHBB7_000286"
     CDS             complement(24729..25673)
                     /gene="mvk"
                     /locus_tag="ACHBB7_000286"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM9208271.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            25989..26648
                     /locus_tag="ACHBB7_000287"
     CDS             25989..26648
                     /locus_tag="ACHBB7_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9208272.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            26733..27638
                     /locus_tag="ACHBB7_000288"
     CDS             26733..27638
                     /locus_tag="ACHBB7_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9208273.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(27640..28272)
                     /locus_tag="ACHBB7_000289"
     CDS             complement(27640..28272)
                     /locus_tag="ACHBB7_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM9208274.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    28423..28548
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            28592..29116
                     /gene="infC"
                     /locus_tag="ACHBB7_000290"
     CDS             28592..29116
                     /gene="infC"
                     /locus_tag="ACHBB7_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM9208275.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            29188..29388
                     /gene="rpmI"
                     /locus_tag="ACHBB7_000291"
     CDS             29188..29388
                     /gene="rpmI"
                     /locus_tag="ACHBB7_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM9208276.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            29441..29800
                     /gene="rplT"
                     /locus_tag="ACHBB7_000292"
     CDS             29441..29800
                     /gene="rplT"
                     /locus_tag="ACHBB7_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM9208277.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            30052..31389
                     /locus_tag="ACHBB7_000293"
     CDS             30052..31389
                     /locus_tag="ACHBB7_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM9208278.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            31437..33566
                     /locus_tag="ACHBB7_000294"
     CDS             31437..33566
                     /locus_tag="ACHBB7_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM9208279.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            33541..34230
                     /locus_tag="ACHBB7_000295"
     CDS             33541..34230
                     /locus_tag="ACHBB7_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208280.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(34638..35251)
                     /locus_tag="ACHBB7_000296"
                     /pseudo
     CDS             complement(34638..35251)
                     /locus_tag="ACHBB7_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(35387..35581)
                     /locus_tag="ACHBB7_000297"
     CDS             complement(35387..35581)
                     /locus_tag="ACHBB7_000297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208281.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(35631..36071)
                     /locus_tag="ACHBB7_000298"
     CDS             complement(35631..36071)
                     /locus_tag="ACHBB7_000298"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM9208282.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(36075..36920)
                     /locus_tag="ACHBB7_000299"
     CDS             complement(36075..36920)
                     /locus_tag="ACHBB7_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM9208283.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            37069..38487
                     /gene="pepV"
                     /locus_tag="ACHBB7_000300"
     CDS             37069..38487
                     /gene="pepV"
                     /locus_tag="ACHBB7_000300"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM9208284.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(38544..39491)
                     /locus_tag="ACHBB7_000301"
     CDS             complement(38544..39491)
                     /locus_tag="ACHBB7_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9208285.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(39714..40064)
                     /locus_tag="ACHBB7_000302"
     CDS             complement(39714..40064)
                     /locus_tag="ACHBB7_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM9208286.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            40264..41343
                     /gene="pepA"
                     /locus_tag="ACHBB7_000303"
     CDS             40264..41343
                     /gene="pepA"
                     /locus_tag="ACHBB7_000303"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM9208287.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            41487..41807
                     /locus_tag="ACHBB7_000304"
     CDS             41487..41807
                     /locus_tag="ACHBB7_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9208288.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            41829..42293
                     /locus_tag="ACHBB7_000305"
     CDS             41829..42293
                     /locus_tag="ACHBB7_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9208289.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            42498..43103
                     /gene="ytpR"
                     /locus_tag="ACHBB7_000306"
     CDS             42498..43103
                     /gene="ytpR"
                     /locus_tag="ACHBB7_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM9208290.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(43142..44431)
                     /locus_tag="ACHBB7_000307"
     CDS             complement(43142..44431)
                     /locus_tag="ACHBB7_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM9208291.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            44859..45338
                     /gene="rlmH"
                     /locus_tag="ACHBB7_000308"
     CDS             44859..45338
                     /gene="rlmH"
                     /locus_tag="ACHBB7_000308"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM9208292.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT        14428 a         8256 c         9727 g        12999 t
ORIGIN      
        1 ctgtcataat gtttttcttt tttttgttat tcataaaaaa aggataaaaa aaagaatatt
       61 aaataaagta gaaggaagcc gagggaactt gcaaaaagca cttgaaacga ttatgatggt
      121 gggagttgtc aagatatttt atatagaaag tagggaaatg aatgacgcaa cctgccattc
      181 gctatcgttt aatcaagaaa gaaaaacata ctggcgcgcg tttaggggaa ttgatcacgc
      241 cacacggaac tttccccaca ccaatgttca tgccagtagg aacattagct acagtaaaaa
      301 caatgtcccc agaagagttg aaagaaatgg gtgctggcgt tattttaagt aatacatatc
      361 atttatggct acgccctggt gaagacttag tagaagaagc tggcggactg cataaattta
      421 tgaactggga tcaaccgatt ttaacagatt caggcggctt tcaagttttt tcattaagtg
      481 atatgcggaa tatcgaagaa gaaggcgtgc atttcaaaaa tcacttgaac ggttcgcgta
      541 tgtttttatc accagaaaaa gcaatcaaca tccaaaataa attgggctct gatatcatga
      601 tgagttttga cgaatgtcca ccattcgatg aaagttacga ctacgtgaaa aaatcagtag
      661 aacgtacgtc tcgatgggca gaacgaggat tgaaagctca tgctaatcca gaccgtcaag
      721 gattatttgg aatcatccaa ggagcagggt ttgaagatct tcgtcgtcaa agcgcgaaag
      781 atctagtcgg gatggatttc cctggttact caatcggcgg gttatctgtc ggggaaccta
      841 aatcagaaat gaatcgtgta ttagaattca ctacaccgtt gatccccgaa aacaaaccac
      901 gttacttgat gggtgtcggt gctgctgatt cattgatcga tggtgttatc cgtggagtag
      961 acatgtttga ctgtgtcttg cctactcgaa tcgctcgaaa tggcacatgt atgacttctc
     1021 aaggccgtct agtagtgaaa aatgcgaaat atgcccatga tttccgtcca attgatgaga
     1081 aatgtgattg ttatacatgt aaaaactata cacgtgccta tatccgccat ttgatcaaat
     1141 gtgatgaaac atttgggatt cgtttgactt cttaccacaa tttatacttc ttgttgaatc
     1201 taatgaaaca agtacgtcaa gcaatcatgg atgataattt gcttgaattt agagaagcct
     1261 tttttgaaga atacgggttc aataaagaaa acgcaaaaag cttctaaagg aaaatctttt
     1321 aaaaattaag taaaaaacta gtgttatcag catttttctg ctaaagtgtt ttttgtagat
     1381 acagaattag tgaggtgaaa aaaatgggaa cattaccaat gcttgtgatg tttatcgcgc
     1441 tattagcaat gtggtttttt atgtcacgtt cgcaaaagaa acaacagcaa gaacgtcaaa
     1501 acttgttaga tagcatgaaa ccaggtgatt cagtcgtaac gatcggcgga ttacatggag
     1561 ttgtttcaga aatcgacaat gacaaacgga cagttgtttt agactgcgaa gggatcttcc
     1621 ttgagtacga tcgtgcagca atcaaaacgg tcaaaccagg gactgtcgta acaaatgatg
     1681 caacgactac tgttgaaaca acagaagaac cagctgagac agttgaagta aaaactgaaa
     1741 caacagaaac gctggaagag acagaagaga aggaagaaaa ataagtatca aagcttattt
     1801 aaagacgagg tagggcagaa agatgtccta cctcattttt tattcaaaaa agaaagagat
     1861 aagagcatgg gatatattgc tcaatattga ataatcgggt gtgtcatata aataataata
     1921 aataacttta tatatttttc tatacaaaca cctgtaaata aagcaatttt atatttaaaa
     1981 ataactgtga aaaaaatcac aaaaaagtgt ttacatagaa aaataaatat tgtatgatat
     2041 acatgtgaat tatttaacaa ataaccatga cccattagga ggataatgat ggctaaagta
     2101 atagaaaaag aaaaaacgaa gacaattgac gtacaagcaa tgattgatga tttggcagaa
     2161 aaagcaaatg cagcattgaa agaaatggaa agctttgatc aagaaaaagt agaccatatc
     2221 gtgcatgaaa tggcaatggc agctttggat caacatatgc cattagcgaa gatggctgta
     2281 gaggaaacag gccgcggtgt ttacgaagat aaagcaatca aaaatatgta tgcgtccgaa
     2341 tatatttggc ataatatcaa acatgataaa acagttggtg tgatcaatga agatgtccaa
     2401 aaagggttga ttgaaattgc tgaaccagtc ggtgtggttt gcggagtaac tccaacaacg
     2461 aatccgactt ccacaacgat tttcaaatca atgatcgcgt tgaaaacaag aaatccaatc
     2521 gtttttgctt tccatccaag tgcccaaaaa tcttctgcag aagcagcacg agtggtacgt
     2581 gatgctgcta tcgctgccgg agctcctgaa aattgtatcc aatggatcga acatccttcg
     2641 atcgaagcga cttctatgct aatgaaccat ccaggtatcg cgatcgtctt agcgacaggt
     2701 ggagctggaa tggtcaagtc tgcttactct acaggaaaac cagctttagg tgtaggtcca
     2761 ggtaacgtac cagcttatat tgaaaaaaca gcaaaaatta aacgtgcagt gaacgatttg
     2821 atcgtttcga aaacatttga taatggaatg atttgtgctt ctgaacaagc tgtgatcgtg
     2881 gataaagaaa tctatgcagc agtaaaagca gaattccaag cacaccaagt ttatatcgta
     2941 aaaccagatg aattgcaaaa attagaagat gcagtgatga acgaaggaaa atatgcggtc
     3001 aatccatcaa tcgttggaca ttctgcgatg gaaatcgcaa aattggcagg aatcagtgta
     3061 ccaaaaggaa cgaaaatgct gattgctgaa ttggaaggcg taggcccaga ttatccgcta
     3121 tcaagagaaa aactttctcc agttcttgcc atgatcaaag ctaacaacac ggaccatgcg
     3181 tttgatttgt gtgaaggtat gttgaatctt ggaggattag gacatacagc agttatccat
     3241 tcagaaaacg aagagttgca tgtcaaattc ggtcttcgaa tgaaagcttg tcgtatttta
     3301 gtgaatacac catctgcaga aggcggcatc ggtgatatct ataacgaaat gattccgtca
     3361 ttgactttgg gatgcggttc ttatggtaag aactctgttt ctcgtaacgt ctctgctgta
     3421 aacttaatca atgtcaaaac tgtagcgaaa cggagaaata acatgcaatg gttcaaatta
     3481 ccgccaaaaa tcttctttga aaagaactct ctattgtact tagagaaaat ggaaaacgtt
     3541 gaacgtgtca tgatcgtttg tgaccctggt atggtccaat tcggttactg cgatacagtt
     3601 cgtgaagtat tggcacgacg taaaaatgat gtgaagatcg aagtcttctc agatgttgaa
     3661 ccgaacccat cgacaaatac cgtatacgca ggaacgaaac tgatggcaga cttcaagcca
     3721 gatacagtaa tcgctcttgg aggcggttct gcaatggacg cagctaaggg aatgtggatg
     3781 ttctatgaac acccagacac atctttcttt ggtgcaaaac aaaaattctt ggatatccgt
     3841 aaacggacat ataagatcga caaaccagaa aaaacacaat tcgtatgtat cccgacaaca
     3901 tcagggactg gttctgaagt aacgccattt gcggtcatca ctgacagtga aacacatgtg
     3961 aaatatccgt tagcagatta tgcattgacg ccagatgtcg caatcgtcga tcctcaattc
     4021 gtcatgtcag ttccagcatc tgtcacagca gataccggga tggatgtgtt aacacatgca
     4081 atcgaatcct atgtttcagt catggcttca gattatacac gtggattgag cctacaagca
     4141 atcaagttag tctttgacta tttagaaaaa tcagtaaaaa cacctgatat ggaatcacgt
     4201 gaaaaaatgc acaacgcttc tacaatggct ggtatggcgt ttgctaacgc attcctaggt
     4261 atttgtcact cagtcgcaca taaaatcggc ggggaatacg ggatccctca tggacggaca
     4321 aatgcgattc ttttaccgca tattatccgc tataatgcaa aagatccaca aaaacatgcg
     4381 atgttcccta aatatgacta tttccgtgca gatacagatt acgcagatat cgcaaaattc
     4441 ttaggtctga aaggcgaaac aacagaggaa ttagtcgaag ctttagcaac ggctgtttat
     4501 gaattaggaa aatctgtcgg catcaacatg agtctaaaag ctcaaggagt cacacaagaa
     4561 acactagata caaccgtaga ccgtatggct gaattagctt acgaagacca atgtacgaca
     4621 gctaatccga aagaaccatt gatctctgaa ttgaaacaga tcatcattga tgcttacaac
     4681 ggataagcaa aagaaagcgt agaaaaaaga taagaaaata gatgaaaaag aacttggaaa
     4741 acgttgtttc tccaagttct ttttttaatt tccaagtaac tgaaagttac ttttgttatt
     4801 taacatttag gtcaatttta gacagttttt gttcaaaaag cgatatttat agtggacggt
     4861 attactgcta tattaaggat ataaaatgaa agcgtttaaa aaggtggcgt aaagatgatt
     4921 attaaagaag agatttttgt acctaaagaa ttagtgaaag aaaaaattga cctgctcgtt
     4981 gaaaacctga caacgatcaa agacgataat ggtgaatttt tgttggactt tgatggactg
     5041 aaagtagatg acaaaagctg gacagtgtgg aattggccac aaggtgtcgg tctatatggt
     5101 atttataaaa attaccagat gacaaaaaat cctcgcgctt atcaagtggt caatgaatgg
     5161 tttgaggatc gtatggaaga aggcgctccg cccaaaaacg tcaatacaat ggcaccgcta
     5221 ttgactatgg cttacttgta tgaagacacg aaagattcac gctatttgcc ttatttagaa
     5281 caatgggcag aatgggtcat gaatgacatg cctcggacaa atgaagacgg gttacagcat
     5341 gccacatatg gaccggaaaa taagaatcag ttgtgggacg atacattgat gatgacagtg
     5401 cttcctttgg caaaaatcgg gaaactgttg aatcgcccag aatatttaga agaagctcgt
     5461 taccagttta tgatccatat caaatacttg atggataaac gaacaggatt gtggtatcac
     5521 ggctggacgt ttgaaggaaa tcataactat gccgaagcat tttgggctcg tggaaactgc
     5581 tggttgacga ttgctattcc agagatcatt gaaatcttag aattatcgaa agaagatgca
     5641 ttgcgcaaat tattgatcga aacacttgaa gcacaagtgc gtgcattaaa aacttatcag
     5701 tctgaaagcg gtttatggca tacgttgttg gatgatcctt cttcttacgt agaatcttct
     5761 gcaactgcag gatttgctta cggtattttg aaagcggtcc ataaacgtta tttaccacaa
     5821 gaatataaag aagtcgctta tcgtgctatc caaggattgc ttgaacaaat cgatgaaaaa
     5881 ggagaagttc aaaacgtttc aatcgggaca ggaatggggg acagtttaga tttttatcgg
     5941 aatatcggaa tcacagcaat gccttatgga cagtcgttaa cggtgctttg tttgacagaa
     6001 ttgctcgtct cttattgtta aaagaaagga aacccatatg aaaaaaacat cactgctgtt
     6061 tgcagcaagt tcgcttagcc taggtctgct aggtctaagc ggctgcggaa ataattctag
     6121 tgtggcaact gtcgcagatg cagatgtaaa tgagatagtt accttgcgct ttgcttatgc
     6181 aagcaacagc cagccagtaa ttgatgcaat gaatgaattt gggcgtttag taaaagaaaa
     6241 aacagatggt tccgtacaga tccagtattt cccagatgga caactgggtg gagaacgtga
     6301 actgatcgaa ttgacacagt caggagcagt agatttcaca aaagtaagtg cctcagcgtt
     6361 ggaaagtttc tctaaagatt attcgatttt ttcagttcct tatctattca atgatgaaga
     6421 acatttcttt aaggtgatgg ataatgaaga aataatggag ccaatctacc aatcgaccaa
     6481 aaatttagga tttaccggat tgacctatta tgattctggt cagcgcagct tttatatggt
     6541 agacggtccg atccatacgc cggaagattt ggagggaaag aagatccgtg tgatgcaaag
     6601 cgaaaccgct atcaaaatgg tagagctttt aggcggttcg cccgtaccta tgggaagtga
     6661 tgaagtgtac acgtctcttc aatccaattt gatcaacgga tcagaaaaca atgaatttgt
     6721 cttgtataca gctggacatg gtggtgtggc taaatactat tcctatgatg agcatacacg
     6781 tgtgccagat atcatcatca tgaatgatgc catcaaagaa cagctaactg atgagcagga
     6841 aaaagccatt gaagaagcag cgaaagaatc aacggttttt gaaattgaag cttttgcaaa
     6901 agcaattgaa gaagaaaaag ctttagcaca agaagaacat ggagtccaat tcaatgatgt
     6961 agataataca ccgtttcgag aggcagttgc cccacttcat gatatattca aaaacgatcc
     7021 tacctacaaa gacctttatc aaaagatcca agagcaagga gcgtagctgt gatgaaaatg
     7081 attttaaaca aggtattaga gatcctggga gctgttttgc ttgttgggat ggtcgctgtt
     7141 gttttatggc aagtattttc cagaacggtt ttaggaaatc caaatacggt cacggaagag
     7201 cttgttcgat tcggtcttgt ctggtttgcc atgttgtctt ccgcgtatgt tgtgggacaa
     7261 aaagcacatt tagccgtaac ggttttaagt gaaaaattaa ctggcaaaaa aagtaaaatt
     7321 ttagaagttg ttgttcaaag tttatttctg gtatttgctg cgatgatcat ggtgtatggc
     7381 ggatggaatg ctgtcacatt gacaatgggg cagatctcgc cttctttaag tattcctatg
     7441 ggatatgttt atctgtcagt acctgtatcg ggggtactca tcattatcta tagcctgatc
     7501 aatctaatcg atacattaag caacaagcca gcaattcaag aaaaaatata aaaatggagg
     7561 aataaaatgg caatagaagc cggattagtg ttattttttg tatttgttgt gttgcttgta
     7621 ttagggatgc cgattgctat aagtattgca ttctcatcga tgatgacttt attgttagtt
     7681 ataccgtttg atgtttctgc ttttagttca gctcaaaaaa tggtgggaag tatcaatagt
     7741 ttttcgctga tcgctattcc gtttttcgtt ctctcgggca ttatcatgaa taatggaggg
     7801 atagctaaga aactagtgga ttttgcgatg ttgttcgtcg ggcgcgtgcc gggggctttg
     7861 gctcatacga atgtgttagg gaatgcttta tttggttcca tctctagctc ggccattgcc
     7921 gcttctaccg cgattggcgg agtgttgatc cctcagcaag tggaagaagg atatgaccgg
     7981 aagtttgcga cagcggtcaa tatcgcgtca gctccgacag gtatggtcat tcctccaagt
     8041 actgctttta tcatgtattc acttgttgct ggtggtgctt cgatctctgc tttgtttatg
     8101 ggaggttatc tcgtaggcgc tttgtgggcg ctaggaatca tgctagtagc attcatcgtt
     8161 gctaaaaaga ataaataccc tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata
     8221 ataaaagagg cgattcctag ccttctattg attgtcatca ttatcggtgg tatcttgacg
     8281 gggatattta cagcagtaga agcttcagcg atagctgtca tttactccct gttgattgct
     8341 atgttttact ataaaacggt aaaactaaaa gatataccta atatgttgtt tcaatcagta
     8401 gcaatgtcag ggacgatcat gttcttattg gctacttctt ccatgatgtc tttcgccatg
     8461 gcgtttaccg gtattcctca ggcaattagt tcgttgatta tgggcgtaac ggataataaa
     8521 tttattattc tgttattagt caatcttgtg ttattactgg tgggtatgtt tatggatgtg
     8581 gcgcctgcta tcttgatttt tacaccaata tttttaccaa tcgtcacaag tgtaggggtt
     8641 gatcctgtac attacgggtt gttttcaatc atgaatctgt gtgtaggctc catcacgccg
     8701 ccagtaggta caggactata tgtcggtgca agtgtaggag gcgtaaaagc agaggcaatg
     8761 ctgaaaccgc ttcttccttt ttatggggtt atcttagtcg tcttgttttt gattacttac
     8821 ttcccgcaaa tcgtcatgtg gctacccaat atgctgagtt aggagaatag ttatgttgaa
     8881 cattacaatt agaggacatg atctttccga agtccaaacg atagaagatc ttgcagaaaa
     8941 aacaaaagaa caagggattc atacactgca gctcgcctta ggtctttcgt ttcctgaaac
     9001 accttctggc gcaaatgaga tcaattctgg gatgggaaat tatgtcaaac gagttttaga
     9061 aaagcaagaa gtgaccgtag gaattttaag ttgctatatc aatatgattc atccagattt
     9121 aacgatacga caagagttgc tgacaaagtt cgagcagtat cttcgctatg cttcttcctt
     9181 tggtgcgtcg atggttgctt cggaaactgg atgtgttttg ccggaaatcc aatatacgga
     9241 agaaaatttc acagatgaag catttgctga agctgtgtcc gttatccgtc gcttggtaaa
     9301 agctggagaa aaataccaaa tgatggtggg gatagaacca ggtttgaatc atccggtgta
     9361 ttctttagca agggtagagc agctgatcca agcagtagat tcggactatc taggaatcat
     9421 tttagatccg accaatttga tcacaagtag tgattatcaa gaacaagtcc aattggtaga
     9481 agaagctttt gaacgttttg gtgaaaagat atgtgcggtt catttgaaag attttcgtgt
     9541 cgaacaggaa aaaatcgtgc cggtaaattt gggagacggt gtgatcgagt acacaaaaat
     9601 caaagaaatc atcatgaaga atcgtccgta cctgtatgtg gttcttgaag aaacaaaaga
     9661 tgatgggatt cgttatggaa gaagtcttct tgaataatgt aaagaagacg ttggttaact
     9721 aatatttaga atggaggggg cggggctaga agatgccacg tctcttcttt tttataagag
     9781 tatataaaag cagctctttt tgattacaaa aaacgtggta taataaaatt gtgcataagt
     9841 gaacaaaagg aggtgcggca catgctccaa aagaaaatca gcggtgaaca agtcaatacg
     9901 atggtttctg atcagtatga aatctatcat gtcaaagatg tcgtcagcca aaataaaacg
     9961 atctatcatc atcatgattt ctatgagatc catgctacat taaagggaga agcgactttt
    10021 tatttgaacg gacgacaatt tacgatcaaa gcaggaagtg tgctgctcat ccattccaat
    10081 gatttgcatc gtatcgtccg ccaatcttca gatgtattcg agcgtgtcta tatctttgtc
    10141 accccttctt ttctagaaag tcgttccacg ccatggtcta atctatcggc ttgttttcaa
    10201 ccaatgggag agaagaggag tcgtgtattg caaatagaac aggaagtatt gaagcaaaaa
    10261 ttatcattta tcgaccatcc tcctgataaa aaaatttacg gtgaagacat aaaatatgaa
    10321 caagctctgg tagaatactt gatttttctc aatcaagttg tacaaaaaga agagagtgcg
    10381 caagaacaga atgctgtagt tcctaatgag cgtgtggaac aaatgttaca atatatctca
    10441 aaaaatttat ctcatccgct ttctttagag gaaatggaaa agcagttttt tgtcagcaaa
    10501 taccatatca cacgagaatt taaaaagtac actggattta cgtttcatca gtatgtaatg
    10561 aagaaaaagc tgatctatgc caagcacttg ttgcgagaat accgcagctc tagtgctgta
    10621 tatagtaaaa gtggtttttc ttcttatccg cattttctga aagcattcaa gagagagttc
    10681 ggcataacgc ctaaagaatt tttgaaacgt gatacacaga aacagttggt tcattatgaa
    10741 cattttgaag atgaacaatg agaatgatca ctaaaaaata accgcagtag ctgctgttgt
    10801 ttacatagaa gcagaaactg cggttatttt atttttatct atatatttca aaatattttg
    10861 aaaaaatgac gtacatatta tatgaatcag ctgaatttac gaaagtctct cggcgatagt
    10921 cctgtttttg ctttgaacag gcggctaaag taagcaaaat tctcaatccc tatttcttcg
    10981 gaaatcattc gaaccgtaaa ttctgtatac cgaagaaggt ctttagcgta agaaagtttt
    11041 ttgctgatca aataatcgat tggtggagaa ccgataaatt tggaaaaatc tttgctcagc
    11101 tgatatttat tcaataagaa ttgttgttct aattgatcaa gtgtaatcgt ttctttgaaa
    11161 tgttcgtcta agtattgctt cagtttgtaa acgtaggcgg gaatatcctc ttcttcgaaa
    11221 tctagctgat acttttgcaa aatcagttca ttcagcaact cgtggatagc tacagaggac
    11281 tggaaatcgg tacgggcatt cgtgccttgt tgcatgtgga tcaattctgt gatcaaatgg
    11341 tggatcttgt tttctgatgg atcccctgtt gaatggaagg tgttatggcc gtcttttgtg
    11401 aactcctgat aaaatgattg gacgttctga ccattcaaat ggatccaatc catttcccat
    11461 ggttcttcgc tgatcgtttg ataacgctga tattcttggc agtcaataaa aaataagtct
    11521 ccagctttta gctgttgctg attatcatta tatgtaagcg aacctttgcc agacaaagta
    11581 aactttatca agtaagaagg taaatgtgct cgttcagtat agtaagggat tggtgctttg
    11641 aagtaaccga tttcttggat ataaaaaaat aaattcctgc ttctttgtgt aggcgtcaag
    11701 atcactcttt ttgagtcttc cgaccatgag tggttggctt tttcccagta atccatgtaa
    11761 acactcccaa cttgtcaaga taatgcaatt atataccaaa atagtgtgaa gcaaaatata
    11821 agattctgta ttatgatagc attgttaata aatgataacg ctttattaat aaagtgctaa
    11881 tcaacaataa agtaaaagag gtggagaaat gaatccaaca tttgttgcca tcgatatcgg
    11941 tgcatcaagc ggacgactaa tgaaaagtac gagggatgca aaaggttctt tgatcttgga
    12001 agaagtacac cggttcaaaa acggctttcg gaacaaagac ggtcacgatc gctggaagat
    12061 cgattatctt atcaaagaga ttttgattgg atttgaaaaa ttaaagcaga cagggattga
    12121 tcgccttttt gcggggatcg atacatgggc agtcgattac tgtctgattg atgaacaagg
    12181 ggatcggata aatgatccga ttgcttatcg ggacaagcgt accaatggag cagtagaaca
    12241 gttttctgaa atctattctc tagataagtt gtatgagcag acagggattc agatccagcc
    12301 tttcaatacc ttgttccaat tgtttgctga agaaaaagaa aatttggcaa aagcacataa
    12361 attgttactg atacctgatt atttagggta tgtctttact ggtaacatgg tgacggagaa
    12421 aactaatgca tctactatgc agctgctgaa tgcagggaca aaagaatggg aaatagaact
    12481 tcttgaaaaa atcggtgtct cgaaggaatt atttccacca ttgacagagc cgggaacgat
    12541 actaggagat ctgcaaaaag aacaattttc aaaatatgat ttgccagatg tgacctttat
    12601 cacagtagca agtcatgata cggcgtcagc tgtattagga acgccaggtc aaggagaacg
    12661 ttggggttat atcagcagtg ggacatggtc gcttttaggg attgaaacaa agatcgccaa
    12721 tgtttctcgc aatgcttttt ctgaaaatta tacgaatgaa tggggtgccc ataacacggt
    12781 gcgttttttg aaaaacatca tggggatgtg gttgatccag gaagttgccc gaatgcaaga
    12841 gtacgagtac agttatgcgg aattggcaga gcttgctgca aaagaaaaac cgctgcagca
    12901 agtgatggaa atcaatgacc ctcgattttt gaatccccca aatatgatag aagagattca
    12961 gcaatactgt tttgaaacag agcagccagt ccctgtctca tcgggagcta tcgctcgttg
    13021 tgtgtatgat aatcttgctc tttgttacgc acaagagctg atcaaagtag aacacttaac
    13081 tggaacgaaa ttggaaaagc tgcacatcgt agggggcgga tcgaataatc gtctactgaa
    13141 tcaattgaca gcagattatg ccggaatcat tgtagaagca ggacctagtg aagccacagc
    13201 aatcgggaat gttgccatgc aaatgctcag tacaggtcag ttttcgaata tagaacaagc
    13261 aagaaatgta atcaaacagt cgtttccatg tgaaactttt tacccagatg aaaataagca
    13321 aaacacgatg gaatggtacg tgaataaaat caaaggagta atcgtatgaa cacgattgaa
    13381 agaaattacg aacaagcaaa agaaaaatat gcaactatcg gtgtagatac agatgctgta
    13441 ctagaaaaga tacaggacat caaaatttct atgcattgct ggcaaggaga tgatgtcaaa
    13501 ggtttcttga caccggatgg cgaattgacc ggtggaatca tggcgacagg gaattttcca
    13561 ggagccgcac gaacacctga agagcttaga caagatctgg agaaggctta ttcgttgata
    13621 ccgggcaaac acaaattgaa ccttcatgct atctatttgg atacagaaga atctgtcgat
    13681 ctaaatgaaa ttgagccgaa acattttgaa aaatgggtag agtgggcaaa aaaagaagaa
    13741 atcggtcttg acttcaatcc gactttcttt tcgcatccaa tgatgaaaga cggctttaca
    13801 ttggctcatc cagataaaga agttcgtgaa tactggatcg aacacgggaa aagaagccgc
    13861 aaaatcgcgg agtactttgg gaaagaaact gggcaaacaa gtatcaataa cttttgggta
    13921 cctgatggga tgaaagataa tccgattgat cgttttaccc cacgggaacg tttaatggaa
    13981 tcactcgatg aaatttttac agaagagtta gatgaacggt atgtacaaga tgcagtagaa
    14041 agcaaactat ttggtttagg cgctgaagct tatacagtcg gttcccatga attttatatg
    14101 ggttacgggt tgactcgtga caaacttgtt tgtctagatg ctggacattt tcatccaaca
    14161 gaagtgattt ccaataaatt atcttcatta gctttattca gcaaaggaat catgttgcat
    14221 gtttcccgtc cagtgcgttg ggattcagac catgtggttt tgatggatga tgaattgcag
    14281 gaaatcgcta aagaattagt ccgtaacgaa ttgttggaaa aaacaaatat cggtctggat
    14341 ttctttgatg caacgatcaa ccgtatcgct gcttgggtca tcggtacgag aaatacacaa
    14401 aaagcactgc tgaaagctat gctggaacca gttgagcgat tgaaagaaat ggagctggct
    14461 tttgacttta cttcaagaat ggcttatacg gaagaattga aagatttccc gtatgccgat
    14521 gtatggaatt atttctgtta caaaaatcaa gttccagttg gtttggattg gctggaagaa
    14581 gtccaggaat atcaaaaaga cgtgttggaa ttaagaaaat aggagtgtgg ctatattgaa
    14641 aaaaatagat attttacaag cgccttatgt gaaagaaatg atcgaaacga cgtcgaatct
    14701 ttatcgcttg ggatgggatg aaagaaatgg cgggaatatt tcttatttac taaaagaaga
    14761 agagatcaca ccgttcttgg atcctgaaaa agtgatccgc cgcatctcga tgatttttga
    14821 tgccagccgt ttagcaggat gctattttat tgtaactggt tcaggaagat attttaaaaa
    14881 tgtagcgagc gaacctgccg agaatcttgg aatcgttcga gttgctgaag atggcaaaac
    14941 actggaactt ttgtgggggc tggaaaacga atgccttcct acaagcgagt tgccaagtca
    15001 tttcatgagt catatcgccc gtttagaagt agacccagat aatcggatcg ttatgcattg
    15061 ccatgctagt catctattag cgatgagttt tacgcatgaa ttagacgaac gttcctttag
    15121 tcgtacgcta tggcaaatgt gtacggaatg cttagttgtt tttccagaag gaatttccat
    15181 cattccgtgg atggttccgg gaacaaatga gattggggaa gcaacagctg aaaaaatgaa
    15241 agaaactcga ttggttttat ggccacaaca tggaatctac gggtcaggaa aagacatgga
    15301 tgaagtcttt ggtttgatcg agacagcaga aaaggcagca gaagtctata cgtacgtcaa
    15361 agcgcaagga ccaatcttgc aaacaatcac ggacgaaaac ctgtggcatt tagcagatgc
    15421 gtttggtgtc actccaaaag caggttattt ggaggaagtt catacgaaag caggtgtctg
    15481 acatggtgaa aaaagctttt tgcatgaaag tttacccaga aaaacaagaa gaatacaaaa
    15541 aaagacatga tgaactttgg ccagaaatgc gtacagcact tcgtgaacac ggggtcatcg
    15601 actattcgat ctatcttgat ccgagtacaa gtttgttatt tggttactta gtgatcgaag
    15661 atgaaaagtt atgggaaaaa atggcaaata catctatcaa tcaaaaatgg tggaagtaca
    15721 tggaagatat catggagaca aatccagatg cctctccagt cagttgggaa ttgatgccag
    15781 tctttgaact gtagcaacaa gcaactgggc aaccggttgc ttggggttct ttcgttttta
    15841 ttgtgaaaaa aaatacaaat taggaggaat agaaaatgag taatcgaatg attttaaatg
    15901 aaacatcgta ttttggaaaa ggagcgattg agtcgatcgt ccctgaattt cagcagcgag
    15961 gattcggcaa agcagctgtc attactgaca aaggattgat cgagcacggt atagcaacaa
    16021 aagtaacaga acttctagat aaagcagcaa tcgcttatgc tttatacgac gggatcgtac
    16081 ctaatccaac gatccaaaat gtaaaagatg gagtggcttt tgttaaagag gcagaggcag
    16141 actgtttgat cgcaatcggt ggaggttcgc caatcgatac ggcaaaagcc attgggatta
    16201 ttttgacgaa tccagagttt tcagacgtca ttagtttaga aggtgttgct gatacgaaaa
    16261 atccatgttt acctatatta gctataccaa cgacttctgg aacagcagca gaagtaacga
    16321 tcaactacgt catcacagat gaagtaaatc accgaaaatt tgtttgtgtt gatcctcatg
    16381 atatcccaat cgtcgcgttc atcgacagcg atatgatgat gggaatgccg aaaaaattgg
    16441 ctgcatcaac aggtatggat gctatgacgc atgctattga agggtttatc acaaaaggcg
    16501 cttgggaaat gactgatatg ctgcatttaa aagcaatcga aatcatcggc cattctttgg
    16561 aagtttctgt agatggcgat caaaatgggc gagaaaaaat ggcattagga cagtatattg
    16621 cgggaatggg cttctcaaat gtcggtttag gactggtaca cgggatggct cacccgttaa
    16681 gtgcatggta caacattcct catggcgtag cgtgtgcagc acttttgcca actgtcatga
    16741 aatataacaa agaatatact ggagaaaagt accgtgagat cgctcttgtt ctagggatta
    16801 aaggagcagc tgaaatgtct ttagaagatg taagagaagc ggcttgtggt gagatcgatc
    16861 ggttgagcaa agcggttgga atcccagaaa ccatttctga attaggtgta aaagaagcag
    16921 atattccagc aattgcagaa gatgctttgc gagatgtatg cacaccagga aatccaagag
    16981 aaacgacagt agaagaaatc attgctttat atcaaagttt gatgtaaaaa cgaagcggat
    17041 cttcaacatt aaattgcctg taaagagaaa tggtagttta tatctggata tctcttttaa
    17101 attgatggta tttccgctat aatttttctt ataaagaaat aggagaatat tatatggaaa
    17161 ataaaaagcc ttatggaact gtattgatca aagcctctaa aatactagac tatttagcgg
    17221 aaaaccaaga tgtctctttg cagacgatcg caaagggtgt gggaatgacc tcttctacag
    17281 tcttgaaaat actagatact ttgttgatga tcggctacgt aaataaaaat tcagagaaaa
    17341 actatcgttt aggtgcaaag cttattcgct acgcgaataa aaacattgaa caaatcgatt
    17401 tggtagagtt gactttaccg tttttagaac agctgcagca aaagatcgat gagactatcc
    17461 acttaggggt attggacaac aatgagatct tgtacgtgaa caaactagat ccaaaacatc
    17521 aaacgatccg catgtcttca aaaatcggaa tcacccgacc attatacaat tctgcgatgg
    17581 gcaaagccgt attagcagaa ttcagtgaag agcagtacac tgattatctt gaaaagcatc
    17641 cgttgatccc ttacacagaa tatacgatca ccaatgcatt acggttgaaa aaggaaatcc
    17701 aaacggtaaa agaaacccac atcgcatttg acgatgaaga aatcgaacgt gatatttttt
    17761 gtataggagc ctcaatcgtg aaaaacgaac aaatcattgg tgcatttagt gtaagtatgc
    17821 cgaaatatcg tttaacagat gagacaaaag aacaaataat cagtgccctc aagcaaacaa
    17881 aaagcgaaat cgagcaaaga attcaaaaat aatgaaaaaa gcgtttgcaa tttttctttt
    17941 ctgccgtata atatgggtat agatttccaa gttataaaat aaaatttcta aataggaaat
    18001 aggtggagaa gatgaaaaaa attgatattt tatctcgatt gaaaaacgcc ggagtgattg
    18061 cggtggtacg cggaaaaagc aaagaagaag ctttgaatgc ttgccacgca atcatcaaag
    18121 gtggtctaac tggtattgag ttgactttta cagtccctca agcagatcaa gtaatcaaag
    18181 aacttttgtc attctacaaa gaccaaccag aaatagtgat tggcgcaggc acagtattag
    18241 acgctgtcac agctcgcttg gctatcttag ctggcgcaga gtatattgtt agcccttcct
    18301 ttgatcaaga aacggcagag atgtgcaatc tgtatcaaat tccttactta ccaggatgta
    18361 tgactattac cgagatcaaa acagcgctga aaagcggggt agatatcgtc aaactatttc
    18421 ctggaagcgc ttatggacca agtatcattt ctgccttcaa ggcgccgatg cctcaagtca
    18481 atatcatgcc aactggcggt gtgagtctgg acaatatgaa agagtggttc gatgctggtg
    18541 tcgtaacagt cggagtaggt gggaaccttt tagcacccgc agctacagga gattttgaca
    18601 aagtaacgga agtcgcgcaa cagtatgcag caaaaatgaa agaaatcaag aggtagctgc
    18661 ttatgggaaa agtagtgaca ctcggagaaa tcatgttgcg attgtccaca gagcagggaa
    18721 cccgtatcga ttcaagtgaa cgattcgctg cccattatgg cggaggggaa gcaaacgtag
    18781 caatttcttt agctaattac ggacatgaag cgatttttgc tacaaaagta cctgataacg
    18841 cattgggaaa agcagtcaaa aagcacttgc aatgttatgg agtagatacg aaacaggtat
    18901 tatctggtgg atcaagacta ggtacttatt atatggaaac tggtagtggc gaacgcgccg
    18961 cctcagtgat ctatgatcgt gccggatcaa gttttgctga aatggatcat ttggaatggt
    19021 cgctagagga gttattccaa ggaatagata tatttcatgt ttcaggaatc accccagctt
    19081 tgtctaaaaa atggcaaaaa atgacgaaac aggtgataga agctgcaaaa aaagctggct
    19141 gcctgatcag cttcgatatc aactatcgtg gaaaattatg gacacagcaa gaagccagcg
    19201 aagtaatcca tcagctttta cctttagtag atatctgctc agctggaaaa atggatgctc
    19261 tttatttgtt aggtatcgag aaagcgccag aagcagaaga acaacctcta atttattatt
    19321 atcaaaaaat gcaggaaaag ttcccgaata ttcaggtgtt ttattcgacc aagcgtaccg
    19381 ttcattcagc aagtgaaaat gaattgaagg ggacattgtg gatgaaccat gaatatgtgg
    19441 aatcccaagt ccatagaatc accccaatcg tggatcgagt aggtggcgga gatgcttttg
    19501 caggtggcgt gctccatggg attttatcca atatgtctcc gcaagaaatc attgattttg
    19561 ctacggcagc ttctgccttg aagcatacga tccatgggga ctgcaaccaa tttagtcaag
    19621 aagaagtcgc acaattttta gcatgcggct ctggaaaaat cattcgatag aaggagtaga
    19681 agaaaatgca agaaatggaa acacgttata cacacagtcc agaagatatc cgtcattatt
    19741 caacggaaca attgagaaaa gaatttttag tagaaaaggt atttgaacca gggaaaatca
    19801 gtttgactta tacgcacaac gatcgaatga tctttggtgg agtgacacct actgaaaaag
    19861 aattagaaat cacattagac aaagaactag gtgttgatta cttcttagag cgtcgtgaac
    19921 taggtgtgat caatatcggt ggaccaggat tcatcgagat cgatgggcaa aaagaagaaa
    19981 tgaaaaaaca agacggatac tatatcggaa aagaaacacg acatgttgta ttttcttctc
    20041 aagatgctga aaacccagct aaattctata ttagttctgt tccagctcat cataaatatc
    20101 caaatgtaaa aatcagtatc gaccaagtaa aaccaatgga gactggtgaa ggcctgacac
    20161 tgaatgaacg taaaatctac caatacatcc atccaaatgt atgcgaaagc tgtcaattac
    20221 aaatgggtta tacgatttta gaaccaggaa gcgcttggaa tacgatgcct tgtcatacac
    20281 atgaacgtcg tatggaagct tatgtctact ttgattatgc caatgaagat acacgtgtgt
    20341 tccacatgat gggcaaacca gatgaaacaa aacatttagt tgtagacaat gaacaagcca
    20401 ttatttcgcc aagctggtcg atccattcag gtgtaggtac aagcaattac tcattcatct
    20461 gggcaatgtg cggagaaaat atcacctata cagacatgga tatggtgcaa atggatcaat
    20521 taaaataagc ataagggaga gaaaatgatg gaatttagta tggatatgtt tcgtttagat
    20581 ggaaaagtag cattagtcac cggtgctgtc tatggtattg gatttgagat tgcacgctct
    20641 ttagctacag caggagcaaa aatcgtattt aataacttga atcaagaatc agttgatgaa
    20701 ggtatagcgc attataaaga agcaggcatc gatgcaaaag gttatgtctg cgatgtgacc
    20761 gatgaagaag cggttcaagc gatggttcaa cagatcaaag aagatgtagg ttcagttgat
    20821 attctagtca acaatgcagg gatcatcaaa cggacaccga tgatcgaaat ggatgcggca
    20881 gacttccgcc aagtaatcga cgtggattta aatgccccat tcatcgtttc aaaagcagtt
    20941 attccggata tgatcgaaaa aggcggcggt aaaatcatca atatctgctc catgatgagt
    21001 gaattaggac gcgaaacggt cagtgcttat gctgcggcaa aaggcggatt aaaaatgctg
    21061 acgaaaaaca tcgcttctga atacggtcaa tacaatatcc aatgtaatgg aattggacca
    21121 ggttatattg ctacaccgca aactgctcca ttacgcgaaa cacaagaaaa cggtgaacgt
    21181 catccattcg atcaatttat cgttggacgt acgccagcag cacgctgggg cgagccaatc
    21241 gatttggctg gaccatctgt cttcttggct tctagcgcat ctgatttcgt taacggccat
    21301 gtcttgtatg tggacggagg catacttgcc tatatcggaa aacaacctta aaagagcttt
    21361 agtacactgt ttagtggaga ggttgtgaaa tcagcggatt gaacgaatac cgaaggacag
    21421 ctgattaaac atcatttatt cgaaaaaaga ggctgggaca gaagcattta actccaagaa
    21481 ataagaagga attcacgaaa attgcttttc aaatttttgt gaatttcagc ttatttccga
    21541 aggagttgct tttacttccg ccgtttgtac gtgtttagag cgtgagacaa aagtgttctt
    21601 tacttttgtc ccacgctctt ttctgtgcgt cttccatact tcactaagtc gatccctctt
    21661 gcttcgcacc aatccttcac tggtccggaa aagatcagag attgttggtg tagggccgcg
    21721 gtattcgttg cgccgaccat agtataaagc agacgaagct cttcttgcca ttgcttcatc
    21781 agtataatag tttcttctac gccgtgattc atcagatgcg tcagtacggt tcctgaagta
    21841 cccacggcat ttgcgccgag acaaagggct ttaaatatgt cataggcatt gcggatacct
    21901 ccggaggcta ggatttccat agaagtgtct gcttcatttg cttcaagcaa tgaagagact
    21961 gtcgattgcc cccagtcagc taaataactc agttcccgct tggagcgacg ggcgttttcg
    22021 atctgggtga agcttgttcc gcttcgaccg ctgatatcaa ctgtatgaac tcctaaagca
    22081 gctagatcat tgagcgtttc tcttgtcatg ccaaaaccga cttcttttac gatgaggggg
    22141 acgtcaatgg ctgtttgagt ttcttgaata agtactttcc aattggtaaa atcacggtct
    22201 ccctcaggca taaccaactc ttgaggtgca ttcaaatgaa tctgtaaggc atctgcgtga
    22261 aacaatcgga tagcttcttt ggctctttcg acagaagtcc ctgcaccgat gttggcaatg
    22321 attttcccgt gaggatattc ttgtcgcata atcgtataag tatcggctaa agaagggact
    22381 ttcaaggcag cgctgactga accagtggca atcatcaaat ctgcttctct tgcgacgatt
    22441 gcaagatctt ggttgatttt ttttgttttt tcgcttccgc cagtcatcgc attgatataa
    22501 aatgggctgc ttagtgtaaa cccaactgcc tgtgtactta gatcgacatc agaaactgct
    22561 atttgcggca aagggttatg gatgatccgt acgaagtcaa attcgttttt ttgtttatca
    22621 tgaaaggctt tagctagtga aacatgttcg tcttttcgat tcatcggttt tcctttcttt
    22681 gatcgctata gacatgtaac ggcagtggag tgatttctgc tttttcccat gcactcatta
    22741 aaggaagaat gccagatttc tggtcaacaa ttacgattcc gcaatcgccc ccacctgcac
    22801 cagaagattt tgcggctcct tcatactgtt cagctaaatt acacaatttg ttcaaagcag
    22861 gcgtttcgat gacgacccca gtgattgcag ataaatcatg cagtaattgt cggttttttc
    22921 gaatcatctg ttggatcaac gttacattat tttctttaaa ccctttgatc atttcgttga
    22981 cacattctgt actatttttt aagaaaagct gataagccac cattttatct tctctcgaac
    23041 ggtgaacttg atcgaccaaa tcagaagtag aggcagggct acccgtccaa ccaatcaata
    23101 aacgtaaatc ttcaggagca atcaatggct caatggatag acctggccaa tccagggcaa
    23161 gtaactcact gatagaatgc tgagtttctt gttcttggag ccaaggatgg tcgaaggttg
    23221 agaaagcgat ccagccacca taacagctag cagcgatgtc gccgcaggaa ccattatctt
    23281 gaactgctaa attggctagt gctgcaatct tgaaaatctc caactgagat aaattcaacg
    23341 cgtaaaaaac attcaaggct ttgaccgttg caacggtcac ggcaccgcta gaacccaatc
    23401 catatttccg gccatttgaa ctgtctaact cacttgttac tttcagatca taaaaggata
    23461 aaagaatgtt tttttcttgt gcatactttt cagtcaagcg aatagcagca aggatataat
    23521 gaaaaggatt ttcccgaata tctaaaacta attctccgtt gcgtcttgtc caacgtacag
    23581 gcatcccact atattgtgca gattggatac ttccgacttt tcgtgcagat tctacagtga
    23641 ctgtcacgaa ttgatcgact gcagcgataa ctgctggatg gcctgtttca acaactgcat
    23701 attctccggc aatatagagt ttgcctggtg cagatacttc aatcataaaa atctcccttt
    23761 attcaataat cgcaattcct ggaccagcat gtgcagtaat tacttgttct ttgctaaata
    23821 atttggtaaa tgtttcttgt acctcagata aatggctgtt ttccactaaa actttgacat
    23881 tagggccagc atccatcgta aagtagcatg gaatgccttg ctttctcaat tgtcgcaccg
    23941 catccatcgc ttttaagctg tcaggagacc agtaagtgaa tggaggctgg gcagctaaag
    24001 tcgttccatg catttttagt ccgtttcttt ccatcgtttc tccgagaagt tggaaatctt
    24061 ttgttttgat tgcttgtttc aattgatata gatccccttc aacggaatct aaccagcctt
    24121 gataaaaact ggatgtttcg actgtccgac gcatcccatt tctgctggac acttcttttt
    24181 tctggtcgtt gatcaaaacg aaaaccattg ctaaatcgtc ttcgaaagaa tcggaaggga
    24241 ctggcttagc gtaagaactt aagtcgtcat ggcctttttc ccattcgacg aaaccaccga
    24301 aaatacttcg gcaagctgaa ccagacccgc gtcgagcaaa tcttgaaaga gagagatcat
    24361 ctaatccaag cttaagagca gtattgcaag cgcctgctaa agcagctagc ccgctggcag
    24421 aggaggctaa tccagctgcg gtaggaacga aattctgact gattactgaa gcttttagtg
    24481 agaggccggc ttctttacga aaaaggtcaa gaaactgact gacttttttt gttgcttttt
    24541 cgtcttgcaa ggtgccatct agataaaatt catctaccat atagctatca gaaaagatga
    24601 cttctgtctc tgtataaaat gcatccaatg ttaacgaaag gctgttgttc attggaagga
    24661 tgagttcttc atttttctta ccccaatatt tgattagagc aatgttggta tatgcgcgtg
    24721 ctttgccttt aaacataggt atgtactcct aatccttgta tccaagtttc agcagcaccc
    24781 gcatcttcaa gtgcttgact gatttcttgg gcttttgttt ttgtttgtgc taatgcaatc
    24841 atacagccac cgcgtccacc gccagttaat ttggcaccca atgccccggt atcttttgct
    24901 gtttggatca atagattaag aaaatcattg ctgattgtca gcttttccag aagtgattgt
    24961 gattcatcca ttgtctgtgc caacgtttct ggtgaatttt caatgattgc ttgttttgct
    25021 tgcttcgtca agtatcctaa tttttggatc atttgtccag tttcatgagg ctgggtttca
    25081 aagaggtgag cgacatcttt gacggcttct cttgtttgtc ctttgattcc tgtatcagcg
    25141 acaatcaaaa aagcatcgat gttcaaagaa aagtagtcga aaggatgccc ttttgtaaaa
    25201 taaatcggct gcaagctgct agtagcggct gcatcgattc cactaggatt accgtgggcg
    25261 attttttccg aaagctggac attttctaat agtatttcac gagacaaagg aaatgctaag
    25321 taatcataaa aagcacgagt gactgctgtg gcgactgcag cgcttgatcc cattccacgt
    25381 tcagcaggaa tcgtgctttc aatggtcagt tgcaagtttt tttggatgtc atgctgtttt
    25441 tttaagtgaa tgaataattt tttgatattt tttaatgcat gaggagcttc ggctaaattt
    25501 cctgaatagt aggaagaaac cagttgatcg tccattgcat ccagctcttc aaggaatgcg
    25561 gtgacttttg ttgcatagaa aggaaaggca atcgctggtt ctccataaac aaccgcgtgc
    25621 tcgcccatca atatgatctt tccgcttgat tctccttggc catagtttgc cataatctaa
    25681 ctccttctca cgtaaaagtg atttttaagc aaaaaataaa gcgcccaaaa cgagttgcgc
    25741 tgcttcacga atttttttgt ccactaaggg tctcaaaaaa cattcctcct ttattttact
    25801 aaaaccatct cagaagtaaa actaagaata cgttttctaa agaaaaaaca cttttttctt
    25861 aagaattttt tgactttttt tcttctttga ctagaaaaaa atacaattga cactactaga
    25921 tattgggggt atatttaaaa aggttggagt taaacacaat atttagtgat gaagtgagct
    25981 ggaggtaaat gagcgtgatc gttttagcag gtacaattgg tgccggaaaa tcaagtttaa
    26041 cagaaatgat ggctgaacac tttgatagtc aggcatttta tgaatcgatt gatgataatg
    26101 aagtcttgcc cttattttat gcaaaccctg aacagtatgc ttttttgctg caaatttatt
    26161 ttttgaataa acgttttgca agcatcaaac aagcaatgaa agatgacaat aatgtattgg
    26221 accgttcgat ttatgaagat tcactgcttt tccatctaaa tgcagatcta ggaagagcaa
    26281 cagaaacaga agttcgtgtg tatgatgaac tgctggaaaa catgatggaa gaacttccct
    26341 atgctgccca taaaaaacat ccggatttat tagttcatat tcgtgtatct tttgatacga
    26401 tgttggagcg tatcgaaaaa cgtggtcgtt cgtatgaaca attgtccttt gatccaagtc
    26461 tatacgatta ttacaaagaa ttgaacagac gttacgatca atggtatgaa gaatacaaag
    26521 aaagtccaaa aatccaaatc gatggagacc gctataattt tgtggaagat cctcaagcaa
    26581 aagaagaagt cttgaaaatg atcgaagaaa aattagcaga gattcgccaa accaaagtag
    26641 ccatttgatt tacagttgct caaataaagg gtaaaccttt atttgagcga cttttttctt
    26701 tatactggaa acaaatgtca ggaggataga gtatgcggga aaaacactat tttaatcaag
    26761 accgtaattt ttggatcaat gtacagcaaa acgatgtgct agctgttaaa aacttaaaag
    26821 aagaatatgg cattagtgac gagatgctga cttattctct agataaaaat gaacgggcgc
    26881 gcgtagagta cgattctttt gatgaagcgt tactgcttgt atcaaatgtt ccgcaccagc
    26941 aaaaagtcga taatcattat gaaactagtc ctatcgcttt tattttaaaa gaagacggtt
    27001 tgtttacatt taccacgccg aataccgagt acgtgattcg actaattcga tcgcttttgg
    27061 aacgaatgcc agacatgagt gtatacagtt tattgtttcg tacattgttt ttgatttctg
    27121 attccttttt tcctttaatc gaggaagtga atagtgaacg tcagcggctt aatctgaaat
    27181 taagagaaaa aacaacgaac aagaatctgc tgcaactttc agatttagag atcggtttgg
    27241 tttacttagt tacaggaaca aaacaaaatg tagtactttt agaacaaatc aaggcattgg
    27301 ctatttatcg caagttgtca gaaaaggaaa aagaagagtt ggacgatgcg ctgatcgaag
    27361 cacgacaagc agttgaaatg acgaacttag cttcgcagat tttagaccag ctctctggca
    27421 cttataataa tttgctgaat aacaacttga atgacacgat gaaatttttg acggtctggt
    27481 cgctgatctt gaccgttcca actattgtga caggattctt cggaatgaat ctccagttac
    27541 cctttaccca ttccgttttc ggatggggaa tcgccttgat catcagtttg gtcttatcta
    27601 tttggatgct tattgcactg tggcggcgga ttcgttgatc tagtctttga tctgcaaacc
    27661 aagggcttgg gcgatcacaa gtgcttgctc ttggttgacg attagtcccg ccaaagaatc
    27721 ttgagataaa gcgattgctg tgaagaaatt agtcgtaaag tctaatccct tcagagaagt
    27781 tcgaaaccag ttcgaaccgg tcaactgatt gtcagacaaa aaaaggtttt tccaagtgac
    27841 ctcataaaat tcgctgtctt ctaattgaca atgatcgaag gagacagctt tcaagtttgt
    27901 actactaaag gaagccatcg aagcaataca atcagtgaaa gtacagtctc gaagataact
    27961 ttcggcaaaa ttcgtacctg ttaatttaca ttgatgaaac acgacttggt ggaaactgcc
    28021 accaatccac tccagattgg aaaaatcaca tttttcaaaa atgacattac tgcattcaaa
    28081 atgacgtaaa actgtttttt gcatcgtaag tttttggata tgactctctc ttaataccat
    28141 atttttggca tcgagatagc tttggtcacc cccgttgatc agaagaccag agaagatctc
    28201 ctcatcttct aaataaaact ctccctttgt aagaagagga agagaaggtg gaataggttc
    28261 gatttttttc atgtgggttc acctcgtttt tctttagtat agcaaagaag cgggaatcac
    28321 tgctagaaag tctgctaatc taatcatttt cagaaaagga agacaaactg tatgaaaaac
    28381 attgacaaaa agattagttt ttgctatact atgatggttg agaaataaaa gcagaagcac
    28441 ccgcttctcg ccttagttga taatgtcact gggctagata gttttcactg gttctattag
    28501 gtgaagtacg cgcgggttct gtaagagaac tcgttttttt attgccgttt attcggtaag
    28561 cttttctttc tctcataata tttggaggtg aatgaccata gcaaaggata tgatggttaa
    28621 cgacggcatt cgtgcacgag agttacgatt gatcggttca gatggtgagc aattaggagt
    28681 taagacaaaa gcagaagcat tgaatattgc agaacaagca aacttggatc ttgtgttagt
    28741 tgcccctggt gcgaaaccac cagttgcgcg aatcatggac tacggtaaat accgtttcga
    28801 acaacaaaag aaagaccgcg aagcgcgtaa aaaacaaaaa gtgatcaatg taaaagaagt
    28861 tcgcttaagt cctacgatcg acttgaatga cttcaataca aaacttcgta atgcacgtaa
    28921 gttcttggaa aagggcgata aagtgaaagc ttctatccgt ttcaaaggcc gtgccattac
    28981 ccataaagaa attggtcaaa aagtcttgaa tcgcttagct gaagaaactg cggatattgc
    29041 aacagtggaa caaaaagcga aaatggacgg acgcagcatg ttcttgacgc tggcaccgaa
    29101 aaacgacagt aagtaatttg aatgactaac tggctgttgg acagccgata gtcggataac
    29161 gaaagatttt gaggaggaaa tatagtcatg ccaaaacaaa aaacacaccg cggatcagca
    29221 aaacgtttca aacgtactgg taaaggcgga ttgaaacgct tccgcgcgtt tacaagtcac
    29281 cgtttccacg gcaaaacaaa aaaacaacgc cgtcaattgc gtaaagcaag catggtttct
    29341 agtggcgact tcaaacgtat tcgccaacaa ctagcaaaaa tgaaataaga agtacaaata
    29401 acagaatctg aagatttatt tttattagga ggaattatac atggcacgtg ttaaaggtgg
    29461 aacagtaact cgtaaacgtc gtaaaagagt gctaaaatta gcaaaaggtt actacggttc
    29521 aaaacacact ttatttaaaa cagcaaaaga acaagtaatg aattcataca actacgcata
    29581 tcgcgatcgt cgtcaaaaga aacgcgactt ccgtaaattg tggattgctc gtatcaacgc
    29641 agcagctcgt atgaatggct tgagctactc aaaattaatg cacggtttga aagttgctga
    29701 aatcgacatc aaccgtaaaa tgttagctga cttggctgtt aacgatgcag cagcatttac
    29761 tgcattggct gaccaagcaa aagaagcttt ggctaaataa gctaaacaat tataaagagg
    29821 aaagaagata acgaaaagtt atgttctttc ctcttttttt atttacaatc ctccatcaaa
    29881 tgaaacagga caataacgta taagtctaaa atagcataat taaggttttt taattgaaaa
    29941 ctgaacattt atggtcaccc tttaatgaaa gcgctaacta cataagttag aatgagtttg
    30001 actgatatat tagtttgctt atcagaaaaa caaaatggac ggaggaataa catgagtgat
    30061 attattattg gtattttgtt agttatctca ttctttttca tggtctggta ctgtgtaaaa
    30121 ggctacaact tgatggtcgg ctttgccatc atggctactg tatggatggg attagcgcta
    30181 gtcggaaaca cgttttcacc aaacccggcg atggaagggc aaggagtaat tgatgtgttg
    30241 acgcatatct atacgacggg gccagctgag tacgccaaat cgatcttggt aaatgtcttc
    30301 tttggtgcct ttttcgggcg tgttctagta gatagcggaa tcgcagctac cctgattcga
    30361 aaggtagttg aacttggagg ggacaagcca agaatcacga tgagcttact atgtattgta
    30421 accgctgtta tattcatgtc gatgacagga atcggaccag tgatttctat tgcagttatt
    30481 gtgttgccaa tcttgatgtc attaggtatt tcagtaccgg ttgcactgtt ctcatttatg
    30541 ggctcgatta tggctggaat ctttgctaat atcgtcaatt tcaaacaata tcaaacgatt
    30601 tatgctggtt tcaatccagc ggctgaaagc tatacttata atgactattt ccaaattggt
    30661 atgattggga tgattgtaag cttagttgtg gtactaacgg tcgctaatat atcaatgaac
    30721 aaaaagaaac gctatgcaat ggcagcaaac gtaccagctg aaggtggcga cgcaccaatg
    30781 atctcatggt tagccgtatt attaccagtt ttaggggtcg ttcttttaga tcttccaatc
    30841 atcttaggtt ttattctagc aggtatctgg gcattgctgt ttactggaaa attgcgcgga
    30901 ggttataaag aaatctgtcg tcagtttgcg aaattattta cagatggcgc agtagatgtt
    30961 gcaccaatgg ttggtttctt gatgacctta gcgatgttca ataattcagc tgcgtatgct
    31021 tctccttatt tttcagctat ttttggagat tggataccgc aatcaccatt agtcttggca
    31081 atcgtatttg ctatattgac accactagga tttttccgcg gtccaatgaa tctagtcggt
    31141 tcaggctcag ctattttagc tgtcgtatta gctgttaatc caacgatgtc gccagccttt
    31201 ttgttcccgt tatttgcaat cacaacgatt gctcctcagc atttagatat tacacaatct
    31261 tgggttgctt ggggattagg ttatacaaaa gtaacttctc gagaatatat gaagaaatcc
    31321 atcccaactg gttggatcat cggagcgatc ttatgcctga tcacgttctt attgtacggg
    31381 aacgcctagt aaacaaaacg tcgataaaaa cgattagatg aatggaggaa aagaaaatga
    31441 aacgttttgt gcgaatggga attgatgttg gcggtacaca tacgaaagca gtcgcaatcg
    31501 ataatgcgac gcacgaaatc atcggaaaat cttcagtgaa aacaacccac gatgatgtcc
    31561 gtggagtagc cgcaggtgtc gttcagtctt ttcaaaattg tttgcgggaa aataatatca
    31621 gtccagaaga tgttgtgttt gttgcacatt ctaccacaca agcgactaat gcactgattg
    31681 aaggagacgt agcaaaagtc ggcgtaatcg ggatggcaaa aggtggatta gaaggttttt
    31741 tagcaaaacg acaaactaga ttgaacgata tcgatttagg gaataagaaa aaaatagaga
    31801 ttgtcaatgc attcttacct gtcaaacact taaatgtaga ccgtgtttca gaaacgattt
    31861 cttctttaga aagagaaaga gcggaggtct tggtttcttc tatggcattt ggagtagata
    31921 atggggaacc agaacgagtt gtttatgaag cagcatcggt aaaatcgatc cctacaacga
    31981 tggcttctga catcacaaaa ttatatggat tgacaagaag aactcggaca gcagcaatca
    32041 atgcatctat tttaccaaaa atgctagata cggcaacctc tacagaagat tccgtacgag
    32101 aagcaggggt caatgtttct ttgatgatta tgcgcggaga cggcggagta atggaaatca
    32161 atgaaatgaa aaaacgacct gttttaacca tgctttccgg tccagcagca tcagtcatgg
    32221 gctctttgat gtacttgaga gcgtctaatg gtgtttattt tgaagtaggc ggaacaacaa
    32281 ccaatatcgg ggtcattaaa aatggacgtc cagcaatcga ttattcaatt gtaggtggcc
    32341 acccaacata tatctcttca ttagatgttc gggttctagg tgtggcaggc ggttcgatgg
    32401 tacgggctaa tcagtcagga atcatcgatg tcggtccacg ttctgcacat attgccggtc
    32461 tagattatgc agtatttacg gagacagaaa aaatcaaagg tcctaaggtt gaatttttct
    32521 ctcctaaaga aggagaccct gctgattacg tcaaggtagt gatggaagac ggagaagaag
    32581 tgactattac aaatacatgt gcagccaatg tccttggatt agtgcaagaa gaacattttt
    32641 cttatggaaa tgtcccatct gcacgaaaag ccatccaagc attagctgat tattgtcata
    32701 cgacggttga agacatcgca gaacagatca tggaaaaatc ctatgctaag attgaaccgg
    32761 tgattttaga attggcagac aagtatcact tggagaaaga tcagatttct cttgtaggtg
    32821 tagggggcgg cgctgcctca ttgattactt actttagtaa taaaatgggt gtgaaatatt
    32881 cgattcctga aaacgccgaa gtgatttctt ctattggtgt agcacttgca atggtccgag
    32941 atgttgtaga acgtatcatc ccttcgccaa gtaaagaaga tatacgatcg ttgaaaaatg
    33001 aagcgatgaa taaagcgatt gaatcaggcg ctacaccaga atcgatcgaa gtccacgtag
    33061 aaatcgatcc acagacttca aaagtaacgg ctatcgctac tggttctacg gaagtgaaag
    33121 caaccgatct aaccaaggag atcacaacag aagaagcact agagcttgct gcagaggata
    33181 tgcgattaaa taaaaatgaa gtttgcttat tggaaaatac acctttcttt tatgtatgcg
    33241 gcgaacagaa tcgttcaaaa aatgctggaa gtcttcggat catcgatcaa aaaggattta
    33301 tcaaggtcca acgtggacat gcttcatgta tgaaaacaac tgcagccaat tatatgacag
    33361 ctgtcgaaca actttgggaa gatatggcag tttatcaaac tgaattgatt gcccgaccag
    33421 aattttatct ctgcttagga gcaagagtct ctgattttac agcgacggat ctggaacaac
    33481 tacagctttt gatggatctg gaagtatcta ctatggaacc ggaggaagaa gtgatcgtcg
    33541 ttgccggaaa tatcaaacaa acataagtta gaagagttac taaacgaatt acaacagatc
    33601 cccgaagaaa tttggggatt ctaccagttt cggcgagatt tattttggaa aaagattcct
    33661 ttgtccaagc agaaaattct tatacaacaa tcaatcgatt gtggtatcga aacggcatgt
    33721 agcatcaaaa aaaagtaccc ttttgccgat gtaggagaaa tttgtgaaca aatggctgtt
    33781 ccaatcgttt cctgtgaatc agagcaaata aacgaacgaa tcacttttgc aacttatgca
    33841 gaagatgaag ggattcgatt gatgacggag ccgttagaaa aattaaaatg ttcaggacta
    33901 gcttcgatct ctaaagaaac agcgcaagct ttgattattg gacatgaact atttcaccac
    33961 atcgaagctt ctgttaaggg aatttatacg caaagcgaaa aaattgtgct gtggcggttg
    34021 cctttttata cacatcaatc aaacattcgt gcattgagcg agatagcagc gatgagtttt
    34081 tcaaaagaga tgaatcaaag cgatttttcg ccttatgtat tagaggcagt cttactttgg
    34141 ccatataatg aaattcgtag ccaagggatc ttagaagaaa taaaaaaaat agaaaaaaga
    34201 tgtacggagt atgatttaga ttacaaataa agagactgtg acaaaagtcg tgtcacagtc
    34261 ctttttttcc gaataaacga tgttctcctg tcaactcctc ggtcacaagt caaaatattc
    34321 aacaaacatg agaagctgtt ttttgaaatt gttccttgtg actcggagat gaacggcagg
    34381 ctcacgacct cttggaaaac ggtgttcaaa agcttgcatc tgcggtaata agtccagcca
    34441 agttaaaaac ataagaagct atttttactt ggctgttctt attacttgat gcagggcagc
    34501 tttttcacaa cctctcaata aacggtgtgc aatcagctga ccctcggtat tagctcaaaa
    34561 aaaacggcaa aatatgagga actattttgc cgtttctttc tctaatactc aggtcaaaac
    34621 gctgattcca aaacctctta tttgatgggt ctgtcctgaa tatcttcaaa ataatcgggg
    34681 taattatgaa tcacgtattc ttgctcctcg ataatcatat gtaaatgcat cgtgcaaaga
    34741 aatccgactt catcaaagtt atgatcaatc attgattgaa gcattgtctg atgttcatct
    34801 aatactcgac cccagtctaa ttctgaaatc gtcaaacgta accatctgaa acggttcaag
    34861 tgcgtactat aactttctag ccaatcccaa atttctcgtt ttccagcaat ttcataacaa
    34921 gttcggtgaa aatcagtatc caaatcaaaa aaagcttttt tattttgagc gattactgcc
    34981 tgatcggttt ttccaacttt tcccgcgtat aacgggctaa tgaagcagat cggatatcga
    35041 tttttgaaac ataagtgccg gactgtggaa tggtttttac aagctcttga ttgcgcagtt
    35101 ggatcagtgc ttctcgaatc ggtgtacgcc cgatagctag ttgttcttct aacactttat
    35161 cggagatttt acttccaggt gctaactcgg aataaatgat ttgtttacgt atagattcat
    35221 aagcttgctg ttgcaaatta ttgataacca tttaaccttc ctttctatgc agatataatc
    35281 tattataaca taaatcgctt acaaatcgtg tgggcataaa aacacagtct gcaaaaaaag
    35341 ccctgatagg catgcctatt agagcctcga aaagaaaaat attgaatcaa tctggtcgta
    35401 cctcttgtat cggtttgaca cgaccgccga ttcttttagc aaaagcttcg gattcttttt
    35461 tgtctttgaa aatgagcaga ttgtctaagt ttttttcccg agtgcgacag ccagttttat
    35521 cgatgtaacc gtgcattact ttaacgacat acatggggat cacctgcttt tttcttttca
    35581 ttatacgaac tactcgacac aatacaagaa aaatgcttag acggtcttta ttaagaattt
    35641 acatcatcca aagaagcgta cgcttggaat aactgatcta actccggatt gactttgctc
    35701 agcttcacta atcgttgcga atcagaaagt aaaaaacagt gtttactact gccagtagtt
    35761 tttaattttt tgtcttcagt tcccacaatt tcgtagctaa aatccaaccg tgtccccgta
    35821 tacttttgga tagttggatg gatgctcact ttttctccgt aatggatcat ttctttatag
    35881 ctgcaattta cttccagaac ggggatgatg atcccagatt cttcgatttt tttataggga
    35941 aaccctagat gtaccaataa ggctacccga gcctcttcaa accagcggat ataattgctg
    36001 tgatgaataa tccccatttg atctgtttca taataaaaag gttctcgcac ataacctgta
    36061 aatttctcca tgactcatgc ctcgttttcc attgattttt tacaaaaata attcatccat
    36121 ttcggaagtg cttcgctgat ttgtgtcggc aaaacaatat attgttcttt tgccagctgg
    36181 tctccaatga aactatgaag ataaatagct gctgcaatcg tttggtcatt ttgaggaaac
    36241 tgggctaaaa atcctgtgat catcccagct aaagtgtcac ccatgccgcc cgttgccata
    36301 gcagcagaac ctaaaggatt ggtgtagaag tgctggtgac cataaatttt tgtctcatga
    36361 cttttcaaaa cgagcacagc accgatttcc tgttgcttct ttttgttttc tttatcccgt
    36421 tgttcttgta tcggtagaga actcaaccgt tgccactcca tttgatgagg tgtaagtaca
    36481 gtttgttcgg gatagggtag caaaagatga ttagcagcaa gtaaacttaa agcagagcca
    36541 tcaatcacca gccattgttc ttttgattgg ttttctagta cttgtttgaa acgttccaaa
    36601 ctcttagaag aagtacccaa tccgggacca atcaagagta catctgcttg ctttattacg
    36661 gaaagaaact ggtcagtatc cgtccaaact acaatcattg cttctggcag atgactgtga
    36721 agagcactcc gatttttttc atcagttact acagtcgtga gacccgctcc gctgtgtacg
    36781 gcagctaaag cactcataat gattgctccg ccatattgtt cattgccgcc aatcaaaacg
    36841 atacgtccaa atgttccttt atgtgaaatt tttggacgag gtgtgatgac tcttttcact
    36901 agttgttcgt ttatcagcat ctaaaaacct ccttttactc attatagcgt atattttttt
    36961 tagagaaaaa cagataagaa aaggaattgt cagatttttt gaactatttt tttctgtaat
    37021 ttgtggtatc atgaacacga agacttaaat aagtggagga ttaactttat gacaattgat
    37081 tggcaaaaag aagtagaagc acgtaaagaa gacttgcttg aagacttgaa aaatctttta
    37141 cgtgtcaaca gcgagcgcga tgactcaaaa gtaacaccag atgcaccatt tggacctgga
    37201 cctcgcgacg cattgaaaca tatgttagct tatggcgaac gtgatggatt taaagtaaaa
    37261 aatgtcgata actatgcagg gcatattgat cttggcgaag gagacgaaac gctagggata
    37321 tttggacaca tggatgtcgt tcctgcagga gatggctggg acacagatcc ttatgaacca
    37381 gtaatcaagg atggaaagat ctttgcacgc ggatcaagcg atgacaaagg tccaagtatg
    37441 gcagcttact atgcgatgaa gatcattaaa gaacttgact tgaaactatc aaaaaaagtt
    37501 cgcttcgtag tcggcagcga tgaagaaagc ggctggggcg atatggctta ttatttcgaa
    37561 catgaagaag aaccagattt tggtttctca ccagatgctg aattcccaat catcaatggt
    37621 gaaaaaggaa atgtttcttt agctttacgc ttcaaaggcg acaatgcagg agattatgtc
    37681 ttgaaatctt ttgtttccgg acttcgcgaa aatatggtgc caggaacagc aactgcagca
    37741 cttcaagtac caagtgcaga tgcagctatc gcgatggaag aagcatttta ccaattcatc
    37801 gaagcaaatc cagtaagcgg aacgattgaa gcggacaata cgtatgtaaa aatcgaatta
    37861 gttggtaaag gcgcacatgg tgcaagccca caatctggta tcaatgcagg ttctttcttg
    37921 gcattgttct tagataacta tgaattccta ggatctgcta aacaatttat ccatgtagca
    37981 gctgcttatg tccatgaaga tttttatgga gaaaaactag gagtcgctta tgaagatgaa
    38041 aaaatgggca aattgacaat gaatgctgga ctatttgctt ttgaagaaaa tggtacagag
    38101 gaagcgaact tcatcaatat gaatttccgt tttccaaaag gtgtaacagt agacggattg
    38161 caaagtgaca tcgaacaaac agtggggcaa gaaggcgcaa cagttactcg aggtgctcgc
    38221 gtaatggaac cacattacgt accaatggat gatccattgg tggctacatt attgcaagtc
    38281 tatgaagacc acactggaga aaaaggctac gaacaaatca tcggtggcgg aacttacgga
    38341 cgtttgttga aacgtggcgt tgcttatgga gcgatgttcc caggatatac tgatacaatg
    38401 catcaagcaa acgaatttat gagtcttgat gacttgttca gagcaacagc aatctatgca
    38461 gatgccatct atcgtttggc gaaataatta attaaaaaga ggatcaagaa ttgcaaatta
    38521 tttttgcagt cttgatcctt tttttacttt ctactgtggt attcatgata aacatgatat
    38581 agattcaatt catattcttt cttagcgttc gtgacgactg tatccaaaat caaccctgtt
    38641 actaatgaaa gaagtgctaa agtcatcaat ccagtactta aaatcgcaga agggatgcgt
    38701 gtaataaagc ttgtcagcat atattcgcga atcacaggaa tccccgtgac caatccaaat
    38761 aaaaagaaga agatggtcca aatcccaaag aaaagcaaag gcttataatc tttaaacatt
    38821 ttaataatca tcatgatcac tttgaatccg tcagagaatg tattcaattt tgattcgctg
    38881 ccttcaggac gatcacgata atcgattggg atttcaacga acttgaattt tttatctaaa
    38941 gcatggatcg tcaattccgt ttcgatttga aaaccagaac tcataatagg aaaacttttg
    39001 acaaagatac gattgaatcc tcgatagccg gtcatgacat caagaatttt tgttttatac
    39061 attctagtaa ttgtatttct aacaaggtta tttccaaaat cgtggaaagg tctcttattc
    39121 tcatcaaagt aggttccatt tgataggcga tcaccaatga ccatatctgc ttcaccagaa
    39181 cgtaatttct cgagcaaacc atgtacagct tctgcaggat aagtatcatc gccatctacc
    39241 attaaataat agtcggcatc gatatcaaag aacatttggc ggatcacatt tccttttcct
    39301 tgacgaggtt ctttttttac gattgctcca ccttgaacgg ccaattcgta tgttcgatcg
    39361 gtcgaattat tatcatagac ataaatatct gcttctggta attctcgttt aaagtctgca
    39421 atgactgttg aaattgttgc ttcctcattg taacaaggaa tcagaacagc aattttttct
    39481 tgttgttcca tttttcactc tccttaacta tagaaaacaa aagctaaatt aaaaatacct
    39541 cataatcaaa ggaaatgcta gtttttcccg ctaaatatca tttatttaaa aagaaaatac
    39601 tggaatatat gtttggaaag ctctatctaa aaaaccaccc actacaaaag aaggacatga
    39661 tacctctctt ttgaatagtg ggtgcgtatt cgttctctca cttttttgac gtattaatct
    39721 gttgcacctt tttttcttga aatttctaat acggttcctg tatgagcatc tgctaaaaat
    39781 tcataataaa cagtttcgcc atcttctatt cgagaaagac ctccccgata agcactcgac
    39841 cgaacggcga atttcctaat aggttccttt tcaaaagaga tccaagaacc ttcgattgca
    39901 ccttctttta gaaaagatga cttgatattt tcaagtactt ggtctgctga caatactttt
    39961 ttgcgatgat accaaatcgt tgaagctacc ccgctcaata atcctagacc gattccaact
    40021 gctaacccgc ctttaaaata gcttagttcg ctttcttcat acatgtggac atacctcctt
    40081 cttctcactt gttactcctt gtgcttattt taccacactc tcatattttc tttgaaataa
    40141 ataaccgcta ccagaaaaaa taagctataa taaatacgat cggtctgcag aaaaaatcat
    40201 attttatccg cagatcaata taagtctgag aggagtttag tctgaaattt agacgaatag
    40261 catatggaag aaaaaacatt tcaacgaatc aaagagttaa cagaattaca aggaacaagc
    40321 ggttttgaac aagatgttcg cgcttatatg gaaagtcata tggaaccgct ggtagatgag
    40381 ttacagttag atggtttggg cggcattttt ggtctgcgcc atcatgaaga agcggatgca
    40441 ccccgtgtaa tggtcgcagc acatatggat gaagtcggat tcatgctgac acaaatccaa
    40501 gaaaacgggc tttttaaagt cgtacctcta ggcggctgga atccttacgt tgtttctgct
    40561 cagcgtttta cacttaaaac ctctactggt aagaattatc catgtatttc ttcttccgtt
    40621 cccccacatt tattgcgagg aacaagcgga caaaaatcag tagaagtaac cgatattttg
    40681 ttcgatgctg ggtttgaatc aagagaagag gcaatgagtt acggtgtcct tccaggagac
    40741 acaatcgttc cttacgctga aacgatcaaa acagccaacg gaaagaatat tatcagtaaa
    40801 tcatgggaca accgctatgg ctgtacaatg gtattagaag ctttagaagc gttgcaaaat
    40861 gaaacattag gtcatacatt gattgcaggc gcgaatgtcc aggaagaagt aggcttgcgt
    40921 ggttctaaag cttctgtcaa taaattcaag ccagatctgt tctttgcagt agactgctct
    40981 gcagcagatg atacagtgac gaaaaatggc acattcggac atttaggaga aggtacattg
    41041 atgcgtatcc aagatccagg tttgatcatg cttcctcgtt tgcgcgagta cttgctggat
    41101 atcgctgaaa caaacaatat cccttatcaa tactttgttt caaaaggcgg tacagatgca
    41161 ggtgctgctc atacacaaaa cgaaggaatc ccaagtacag tgattggcgt agtgggacgt
    41221 tatatccata cgcatcaaac gatgttcagt attcgcgatt ttgaagctgc acgagaaatg
    41281 ttgattcaaa cactgaaagg tttggataaa tcaacagtga atacgatcgt atacggaaaa
    41341 taatgcgata gtgaaactat aaaatctggc aaaagattgc tttttgccag atttttttta
    41401 taaaaagagg atcaatgttt gttagttcga aagaaaggtg tataatttaa ttgaaaaata
    41461 cataaacaga aagtagagga caaagaatga ttatcccaaa tagtttagaa gaattagcag
    41521 gctacgtaga aactggaaag agtgtatttt tctttactgc cggctggtgc ggagactgcc
    41581 gttttatcaa accgcaaatg cctgaaattg aaaatgactt ttctgattgg caatttattg
    41641 aagtagaccg tgacaagtac atagatgtag ccgctgaatg gaacattttt ggtatcccaa
    41701 gttttgtcgt gatccaagac ggaaaagaat taggtcgtct agtgaataaa gatcgtaaaa
    41761 caaaacagga aatcgaatcg ttcctagaaa gtgtaacaaa aggctgacat ctgttaggag
    41821 gggtcaagat gaatcaagaa tataagaata ttttagtagg tatcgacggt agtgaacaag
    41881 cacttgctgc tttcaaaaaa gcggtagaag tggctcgccg taataatggg aaagtatatg
    41941 tagcaaacgt aatcgatcag caattttata actttatggg ctatgcacct attgatcaaa
    42001 atctagtcga tcagcaaact gaaacagcca aggaaatgat cgaagaatgt aaagaatatg
    42061 ggaaatctgt tgattacaca aatatagaag gaattattgc atatggttct gttcgtgaat
    42121 ccatggcagt caaattgcca aaaaaatatg agatcgatct aatcatggtt gggcagtcag
    42181 gtctaaatgc agtcgaacgc tttatgacag gaagtgtagc cagctacgtg atacgtcatt
    42241 caccttgtga tgtcttgatc gtttctgacg aagaagaaca gccaagtaat taagcaagtg
    42301 gaacttgtac gaccttttta atcaattaac taaaacagta aagggactgt gacaaaagtc
    42361 ttgtcacagt cccttgttac gaataaatgg tgttccagaa gaggcttctt cggaaataaa
    42421 gcatgaaaaa aatttcatgc agtgaatgca attttttgtt aagataaatc tcagcaaagg
    42481 ataatggagg aaagaacatg atttttgctt ataacaaagc acacgtcgga gacaccttga
    42541 tggttattat agccgacgat aaaggaacag aaaacactgt tgaacgcaaa tggaatgttg
    42601 ctcggatcaa agatgaacaa ggtcaagttg ttgcttggaa ctttttccat atctccgatc
    42661 atctaacaat cgaagggaac ggccaagtaa gcttaacaga tgaggaatta gcgaaattga
    42721 atcgattgat caaagaagca ggattcgaag aacagcttga agctgatcat gaaccgaaga
    42781 tcgttgtcgg gtacgtaaaa acatgcgtcc cacatcctga ttctgaccat ttatcgatta
    42841 ctgagacaga agtagacaat ggtacagtat tgcaaatcgt ttgcggtgca cccaatatcg
    42901 aagctggaca aaaagttgtc gttgcaaaac caggagctat gatgccagat ggaatgatga
    42961 tctggccagg aactttgcga ggggtagata gctatggaat gatctgttcc gctcgtgaat
    43021 tgcatttgcc aaatgctcca gcaaaaaaag gaatcttagt attgcctgag gatgccgtag
    43081 tcggagaagc atttccaaaa taaagaggtt gtgacaagtc tttgtcacaa cctctttttt
    43141 attaattttc actattttga ttttgtttta atgccgattg atcgatcgtc aattcaacag
    43201 tagttgtttt ttctttcgag ccttcatagt aagtgatttt gacagaatct cccacttttt
    43261 tcttatacaa agcagattga agttctacac cagaagaaac ttctttgtca tcgatcttag
    43321 taatgacgtc gtattgtttc aatccagctt tttcagccgg tgtagctgtt tggacatttg
    43381 tcacgatgac accgttagtt acagatgaag gaatcttcaa gatttgttct tgttgctgag
    43441 tagaaacggc agataagtcg accattgtga tacctagagc tggacgtgtc acttttccgt
    43501 ctttttccag ttgattgatg atattcacta catcgttact tggaatagca aatcccattc
    43561 cttctacgct tacattagaa gtagattcag aagtacttgc gatcttactt gagttgatac
    43621 cgataacttg tccttcgata ttaactaaag gaccaccaga atttccaggg ttgattgctg
    43681 catctgtttg gatcgcattg atattgactg tttcgttcga ttcattcgta cttgtcactt
    43741 gtcggttcaa ggaagaaatg attcctgaag tgacagaatt tgcatattca gatcctagtg
    43801 gggaaccaat cgcaatcgca ggctcaccaa ccttcagagc acttgagtca ccaaaagagg
    43861 caactgtttc gactttgtct gaattgatct tcaacaccgc taaatctgaa taagcatctg
    43921 ttccaactaa ttcagctttt accttcgtac cgtctttcat caagacttca agaccttgct
    43981 ggccatctac tacgtgatta ttcgtgacta cataagcagt attcccgtct tttttgtaga
    44041 tcacgccgct tccttcacta taagcttcca aattactatc atctgagctg ctactttctt
    44101 cttgctgccc gaatagtccg ccaaaaccgc ttgattggtt ttgactttgt aagttgataa
    44161 cagaaacgac agcgtcttga actttatcta ccgctttggt gatatcagaa tctacattga
    44221 ctttgacatt ttccacaact gtttcaccgg cagaattctg attgccgctt gtagcagtag
    44281 aactattgtt gccagtgccc atcgctgcat aaaaaatgcc agctgtcagt agtcctccaa
    44341 cgatccctcc aacaagcccc agacctaact tacgccagag gctattattt ttactttttt
    44401 tcatctttgg tgtcacattt tttctatcca tgtgttcctc ctccaattac attcatttat
    44461 ctactgttag tatagtctta tttgttgact ttagtctact ttgagactgt gaacttttta
    44521 tgaaaaactt taggaaaaat cattgttttc agatgaaaat atttgatttt ctttttccac
    44581 agatactgtg taaaactacg cggataggat tggaaaaata agggataaaa catatccaca
    44641 gcctgtgtat aaagtggata aattcttata aaacgtatgt ttgcctaatg aatgtgatga
    44701 aataaggtaa aataacactg ttttctgtgg ataaacctgt ggatgttgtg cataactgtt
    44761 ggggaaatct ttttaacaaa aatagccaaa ataaacatat agacgaaaaa attgattata
    44821 ataaacaaag tgagcatgaa gaccaaagga gagaaaacat gaatatcaaa attatctcag
    44881 taggaaaatt aaaagaaaaa tacctgatcc aaggtatcaa tgagtatgtc aaacggctga
    44941 atgcctatgc aaaaatcgaa ttgatcgaag ttcctgacga aaaagccccg gagaatttaa
    45001 gcgaagcgca aatgagacaa gtaaaagaaa aagaagggga acggatctta gcgaaaatca
    45061 aagaacaaga atacgtctat gccttagcaa ttgaagggaa aaatcctaca agtgaatctt
    45121 ttgctaaaca aatcgaccaa ttaggtatcc aaggaaaaag ccaccttgtt tttgttatcg
    45181 gcggctcttt aggactcagc gaagccgtca tgaaaagaag caatgctcag atctcttttg
    45241 gaaaaatgac gtatccacat caattgatgc gtctgatcct tgtcgagcaa atctaccgtg
    45301 ccttccggat caatgcgggg gctccgtatc ataaataatg atatttaaga ttgaatgctc
    45361 ctgaacacta agttagcttt tcattgaaat ttatatataa aaaagttaac
//
LOCUS       DAYYKN010000007        44863 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000007 DAYYKN010000000
VERSION     DAYYKN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.007"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..1012)
                     /locus_tag="ACHBB7_000309"
     CDS             complement(2..1012)
                     /locus_tag="ACHBB7_000309"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9208293.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1192..1536)
                     /locus_tag="ACHBB7_000310"
     CDS             complement(1192..1536)
                     /locus_tag="ACHBB7_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM9208294.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1529..1963)
                     /locus_tag="ACHBB7_000311"
     CDS             complement(1529..1963)
                     /locus_tag="ACHBB7_000311"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM9208295.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2101..2838
                     /locus_tag="ACHBB7_000312"
     CDS             2101..2838
                     /locus_tag="ACHBB7_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9208296.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2831..4042
                     /locus_tag="ACHBB7_000313"
     CDS             2831..4042
                     /locus_tag="ACHBB7_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9208297.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4246..5028
                     /locus_tag="ACHBB7_000314"
     CDS             4246..5028
                     /locus_tag="ACHBB7_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM9208298.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5098..5760
                     /gene="trmB"
                     /locus_tag="ACHBB7_000315"
     CDS             5098..5760
                     /gene="trmB"
                     /locus_tag="ACHBB7_000315"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM9208299.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5858..7612)
                     /gene="ade"
                     /locus_tag="ACHBB7_000316"
     CDS             complement(5858..7612)
                     /gene="ade"
                     /locus_tag="ACHBB7_000316"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM9208300.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7744..8673
                     /gene="comGA"
                     /locus_tag="ACHBB7_000317"
     CDS             7744..8673
                     /gene="comGA"
                     /locus_tag="ACHBB7_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM9208301.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8630..9679
                     /gene="comGB"
                     /locus_tag="ACHBB7_000318"
     CDS             8630..9679
                     /gene="comGB"
                     /locus_tag="ACHBB7_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM9208302.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9676..9954
                     /gene="comGC"
                     /locus_tag="ACHBB7_000319"
     CDS             9676..9954
                     /gene="comGC"
                     /locus_tag="ACHBB7_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM9208303.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9929..10399
                     /gene="comGD"
                     /locus_tag="ACHBB7_000320"
     CDS             9929..10399
                     /gene="comGD"
                     /locus_tag="ACHBB7_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM9208304.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10371..10718
                     /locus_tag="ACHBB7_000321"
     CDS             10371..10718
                     /locus_tag="ACHBB7_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208305.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10681..11106
                     /gene="comGF"
                     /locus_tag="ACHBB7_000322"
     CDS             10681..11106
                     /gene="comGF"
                     /locus_tag="ACHBB7_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM9208306.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11078..11437
                     /gene="comGG"
                     /locus_tag="ACHBB7_000323"
     CDS             11078..11437
                     /gene="comGG"
                     /locus_tag="ACHBB7_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM9208307.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11609..12616
                     /locus_tag="ACHBB7_000324"
     CDS             11609..12616
                     /locus_tag="ACHBB7_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9208308.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12641..13825
                     /locus_tag="ACHBB7_000325"
     CDS             12641..13825
                     /locus_tag="ACHBB7_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM9208309.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14262..15845)
                     /gene="abc-f"
                     /locus_tag="ACHBB7_000326"
     CDS             complement(14262..15845)
                     /gene="abc-f"
                     /locus_tag="ACHBB7_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM9208310.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15950..16078)
                     /locus_tag="ACHBB7_000327"
     CDS             complement(15950..16078)
                     /locus_tag="ACHBB7_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208311.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16423..16566)
                     /locus_tag="ACHBB7_000328"
     CDS             complement(16423..16566)
                     /locus_tag="ACHBB7_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM9208312.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16789..17268)
                     /locus_tag="ACHBB7_000329"
     CDS             complement(16789..17268)
                     /locus_tag="ACHBB7_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9208313.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17341..17625)
                     /locus_tag="ACHBB7_000330"
     CDS             complement(17341..17625)
                     /locus_tag="ACHBB7_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208314.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17703..17897)
                     /gene="ssrS"
                     /locus_tag="ACHBB7_000331"
     ncRNA           complement(17703..17897)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHBB7_000331"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17969..19255)
                     /locus_tag="ACHBB7_000332"
     CDS             complement(17969..19255)
                     /locus_tag="ACHBB7_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM9208315.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19393..20064
                     /locus_tag="ACHBB7_000333"
     CDS             19393..20064
                     /locus_tag="ACHBB7_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM9208316.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20030..20518
                     /locus_tag="ACHBB7_000334"
     CDS             20030..20518
                     /locus_tag="ACHBB7_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM9208317.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20531..21478
                     /gene="prmA"
                     /locus_tag="ACHBB7_000335"
     CDS             20531..21478
                     /gene="prmA"
                     /locus_tag="ACHBB7_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM9208318.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21479..22243
                     /locus_tag="ACHBB7_000336"
     CDS             21479..22243
                     /locus_tag="ACHBB7_000336"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM9208319.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22476..24689
                     /locus_tag="ACHBB7_000337"
     CDS             22476..24689
                     /locus_tag="ACHBB7_000337"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM9208320.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24700..25146
                     /gene="dtd"
                     /locus_tag="ACHBB7_000338"
     CDS             24700..25146
                     /gene="dtd"
                     /locus_tag="ACHBB7_000338"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM9208321.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25317..26576
                     /locus_tag="ACHBB7_000339"
     CDS             25317..26576
                     /locus_tag="ACHBB7_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM9208322.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26665..27612)
                     /locus_tag="ACHBB7_000340"
     CDS             complement(26665..27612)
                     /locus_tag="ACHBB7_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9208323.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28006..29298
                     /gene="hisS"
                     /locus_tag="ACHBB7_000341"
     CDS             28006..29298
                     /gene="hisS"
                     /locus_tag="ACHBB7_000341"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM9208324.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29313..31082
                     /gene="aspS"
                     /locus_tag="ACHBB7_000342"
     CDS             29313..31082
                     /gene="aspS"
                     /locus_tag="ACHBB7_000342"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM9208325.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31191..32066
                     /locus_tag="ACHBB7_000343"
     CDS             31191..32066
                     /locus_tag="ACHBB7_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9208326.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32332..34215
                     /locus_tag="ACHBB7_000344"
     CDS             32332..34215
                     /locus_tag="ACHBB7_000344"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM9208327.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34290..34574)
                     /locus_tag="ACHBB7_000345"
     CDS             complement(34290..34574)
                     /locus_tag="ACHBB7_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9208328.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34564..35298)
                     /locus_tag="ACHBB7_000346"
     CDS             complement(34564..35298)
                     /locus_tag="ACHBB7_000346"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM9208329.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35427..36050
                     /locus_tag="ACHBB7_000347"
     CDS             35427..36050
                     /locus_tag="ACHBB7_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM9208330.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36242..37363
                     /locus_tag="ACHBB7_000348"
     CDS             36242..37363
                     /locus_tag="ACHBB7_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM9208331.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37370..40498
                     /locus_tag="ACHBB7_000349"
     CDS             37370..40498
                     /locus_tag="ACHBB7_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9208332.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40517..43711
                     /locus_tag="ACHBB7_000350"
     CDS             40517..43711
                     /locus_tag="ACHBB7_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM9208333.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43857..44564)
                     /locus_tag="ACHBB7_000351"
     CDS             complement(43857..44564)
                     /locus_tag="ACHBB7_000351"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9208334.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14395 a         7965 c         9409 g        13094 t
ORIGIN      
        1 tttattttga tgaatcagtt gtttcttcag ttgaagattc ttttgttgaa tcagttgatt
       61 ttgtgtcaga actctttgtt gaagctgtag tttcacttga gtctttcgta cttgatgaag
      121 ttgttgtgaa tgcactcaac acattttcaa aggcatcatc tttgattttt acatttgcat
      181 cttttaattc ttcaccgatt actttagttg tgaatgcatt gtcacttagt ttagtttcag
      241 tagcgatatc ttttagttga tctttgtatt tatccatgtc attgccttta ttttggtttt
      301 tcaccatttt gacaacgtag tattctgtcg cataagaagt aggatttgtt gtagtaataa
      361 catcggagat ctcaccgtct ttcaatttga aagctgcttc ttttacttct gcaggaatag
      421 ttgttgtcgt tgagtcgaat ttaactttac cgccatcttc ttttgtctct gtatcagttg
      481 atttgtcttt ggctaattta gagaaatcat ctccatcatc cgctgatttt ttcacatctt
      541 ttgcttcatc ttcacttgat aatttgatga tttgtgcttc tacttctgga tggaaagatt
      601 tccaagcagt tttcaaatcg tcatctgtaa tatctacgtg agctttcaag ccagcttcaa
      661 aagctaaatt gttacgaata tattctttat aagtatcttt cgtatatcca gcagcttcta
      721 attgtgattc aaaagtatca cctaatgatt tcgcttgttt gtcgtattct gcatccactt
      781 gcttatcggt tactttatcg ccatatttgt tattgaatac tttataaatg atcatccgtt
      841 gtactaagga ttggttcgat gattcaagtt ttgcttcatc atagaaatca gatacagtga
      901 tcgttccgcc tttcatcgtt gcgatatctt tattagtatc gcctgaacaa gctgctagag
      961 ttaaaacagc caacgcacta gtagctgcca aaataattga tttttttttc attaaaattt
     1021 gcactcctgt aattagttat tatttttata tattaactat accatatccc atttccttta
     1081 aaaacgttgt cgtggtagtt tcaaacaaaa ttcaaaattt catcacattc ctaggaaata
     1141 cgcaaacttt tctcacttat atagttaaat ctgttgagaa aataatcatt attatatttg
     1201 ttcaggatac tctggcagat cagcttttag tttttctact tgttcattga tttgtgtgat
     1261 acgtggttcg gcttggaatt taaaatcttc gatatctttt ttgattgctt cttggaaagc
     1321 aggaatcaca gattcagctg tttcttttgt ttcgatcaat gcgcttttaa aatgattcag
     1381 gctgttattt aaatcatttg tcagagttgt tgcttcttcc aattcttcga tcagtttctt
     1441 tcttgtttca ttcccgctcc tgggcgccaa taacagacct ccgacagtgc cagcagcagc
     1501 tccaaataac agtcctttga aaaagttttt aatcatcgca ggacacctgc tttacgattg
     1561 tttcttgaat agcttttagt tcttccgatc cataactatc ttgatgattg acaaagtgaa
     1621 tagaaaaatc atcctctttg ctgtagcgag ggactaaatg gatatgtgaa tgaaaaacag
     1681 attgataggc tagttcacgg ttgttattca agatattaag acctttcatt tctggaaaag
     1741 ctttttccaa tgcttgagca atcttaggca cacgcgaaaa aacctctcct gcaagttcgg
     1801 gatcatattc aaaaatatct gctacatgac gtttaggcac aactaatgtg tgtcctttcg
     1861 ttgcttgaga gatgtctaaa aaagcataga ccttgtcgtc ttcatatatt ttatagcttg
     1921 ggatttcttg attgatgatt ttgcaaaaaa tacagttttc catataaaac atcctttcta
     1981 taaagaataa atacttcagc ttaactttac cataagttaa agctattgta aggaaaaaaa
     2041 caaaataccg tcattttctt cgtggtataa taaataaaaa taaatggcgg aggaaaaaga
     2101 atgagtttaa ttatcgatca tgtcacaggg gggtacggac atctccctgt gttaaaagat
     2161 atcagtttcg atgtacagcc tggagagata gtagggttga ttggactgaa cggagcaggg
     2221 aaaagtacga ccatcaagca cattatcggt ctgctggaag cttcaaaagg aacgatagaa
     2281 atcaatcatc tatccttaaa gaaaaatacg gaagaatatc gcaaacaaat tgggtatatt
     2341 ccagagactc cttctttata tgaagaattg acattgagag aacatatcga agtcactgcg
     2401 atggcctacg atatacctat ggatatagca atgaaaagag cagaaaagtt gttggagact
     2461 tttcggttat ctaatcgtct tgactggttc ccagctaatt tttcaaaggg aatgaaacag
     2521 aaagtgatga tcttatgcgc ctttttgatc gagccttctt tatatatcat cgacgaacca
     2581 tttttaggct tggatccact agctatcaat gcattgcttg aattgatggt agaaatgaaa
     2641 aaagaaggag ccggtatttt gatgtctacg catattttag ctactgcaga aaaatattgt
     2701 gatcgttttg tggtcttaca taacggagag atacgagcag acggttctct tgagatgttg
     2761 agaaaagaat ttcagctgcc ggattcttct ttggacgaaa tctatattgc tttaacaaag
     2821 gagcaggcac atgagtagtt tttttcaaaa acgtttagca agacatcaaa agaaaatgat
     2881 gaaatatatg cgttatgtat tgaacgatca ttttgtttta gtttgtcttt tcttgattgg
     2941 cggtgtggga ttatattatt ctaattggtt gaaaaccctg cagccaccgt ttgcaattgg
     3001 agggctcata atctctgtat tttggatgct ctgtttattc gtgggtaaga tggctacttt
     3061 tgcagaggca gcagatattg tttttttact tcctaaagaa aaagaaatgc aaacctattt
     3121 ggaacgagct tttcgctatt cttgcatttt tccgttgatc tttttgatat tggcatgcgg
     3181 attggccatg ccattagtgg tggttagtac ggagcaatcc ttttcgatgt ttttcgtcta
     3241 tctgatcata ttatggagtt tgaaattcag tcatctgcaa atcaagagag cagaattgtt
     3301 tcgactagat caacaagtta ttcgttattg gaaattaggc tggttttttt cttctctgat
     3361 ctttttgttt ctatcatttt attggctaat atgggcagga atagcaggtg cattagtaca
     3421 ggcaagtcta tattactggt ttttgtggaa gcaacaagaa aaaggattgg attgggagaa
     3481 aatggtgcat gtagaagaac ataggttgca tcagatctat caatttatca atttattcac
     3541 agatgttcct gaaattacgg caaagatcaa aagaagacgc tatttggatc ctatattgac
     3601 tcatattaaa agagaatcaa aaaacactta tctttacttg tttgcaagaa gattggcaag
     3661 aggttctgat tttagtggtc tttatttccg attagtaatt ataggtgggt tattgattgc
     3721 cggagtagtc gacttttatt ttagtctgat tatcggtttc ttatttttat atttgatcgg
     3781 tttccaactg ttacctttag cgaaccagtt ccgctatatg gttctcgttc agctttatcc
     3841 aatccataag aatcagaaga agcaggcgat tcagtatcta ctcacatggg tattaattat
     3901 cgcgtcggtt atttttggtt tgattgctgc agtcgtatta gatgggcagc agcgatgggt
     3961 tccgctagcg gcttatttga ttgttactat gctctttgtt aaactatatg taccaactcg
     4021 tttgaagaag atgtcagact gaaaacaaag ctgtgtaggg taaaaatgag aatgaaaaat
     4081 agagcgttga cagtaagaat gctgttcgtt ctattttttt tgagaaaagc tggttttttg
     4141 aatggcaatt aaatgaaaaa gaacgtatat gtgcttgacg tgtagtgttc aggcgactag
     4201 aataataagt actgcaaaac aaagagtaag aggacgggag aatgcatgat ggagtttcag
     4261 ttagaccaag agtggcggtt acgtcccatc aaaggcgaca caggaaaaac atacataggc
     4321 atgcgtgatg aggacaaagt ctttattaaa cgaaacacga ctccgatgtt ggctgctctt
     4381 tcaagggaag gaattacacc aaaacttgtt tggacaaaac gaacggggaa tggcgatact
     4441 ttgacagcac aagagtggct ggaaggtcgc ttgctgactc ctaatgagat tggtcaaaga
     4501 aacgacgtga ttgatgtttt gtaccattta catcattcaa attctcttaa aaccatgctg
     4561 acccgtatcg gtggtcgtgt catgacgccg gagaagatgc tgcaaaaata tgaaaaagaa
     4621 ttaccggaag aactccagaa aaatcaattt ctgcaaattg tccatcgttt tcttcgtgag
     4681 cagatcccgt caatagataa ggaaaactta tcagtagtac acggagatgt aaaccatcgt
     4741 aattggttag tatgtaaaaa ttatttatat ctagtcgatt gggattcaat catgtttgca
     4801 gaccctgcgg tggatattgc aacgatttta ggaaattatg tgccactgtc tagctggaat
     4861 cagtggctga tcagttatgg catacgcccg actgacgaaa tgcttgaaaa atttcattgg
     4921 tatgcggcca tgaatttgct ccaagaaatc accagatatt gcaaacgtga agatcatcga
     4981 cggatgaacg aagaaatttt acaattaaaa cgaattttca gtggctgagg tttggcccaa
     5041 actctgcgga gatttggcca gaccttttta attagaggaa aggaatggag ataaataatg
     5101 cgtgtccgaa atcgtccggg agcagcggaa atgttagcgg ctcacccgaa ttttgtaatt
     5161 agtgatccaa cattgtggaa gggaaaatgg aatgaattgt ttgaaaacga tcatccaatc
     5221 cacattgaga ttggtatggg gaaaggccag ttcatcactg gaatggcaaa agcccatcct
     5281 gaaatcaatt atattggagt agaaatgcag gtcagtgttg tttcgattgc actagacaag
     5341 ttgatcgaac aaccgcttcc taacctgaaa cttctacatg tggatggatc agcgttgaca
     5401 gagtatttcg ctgacagcga agtagatcaa atctatctga atttttccga tccatggcca
     5461 aaaaaacgtc atgaaaaacg gcgattgaca tataagactt ttcttgctgt agatgaacag
     5521 attcttcgtc caaatggaga aatccatttc aaaacagata atcaaggatt gtttgagtat
     5581 tctctggcaa gtttttctca atatggcatg atcctcaaac aggtatggtt agatttacat
     5641 caaagtcaat ttgaaggcaa tattatgacg gagtatgaag aaaaattttc ttcaaaaggt
     5701 caacgtattt atcgagtaga agctcaattt caagataaga ccaaaaagaa aagtctgtga
     5761 aactaacttt ctgctttcat aaaaacatta atatccataa ataaatccca gaaaatgaac
     5821 attacagatg taatgtattt tctgggattt atttgattta aaatgtattt atttcatcgg
     5881 tttttccaca aggtggaatc aattggaagc gagaaaatga aaataatcct ttgtcggtga
     5941 tcttcaattc gggtatgaca gataatgtta agaaagaaag tgtcaaaaaa ggatcaaagg
     6001 ttttagctgc tcctagttga tgtgctttag ttattaattc attcaactgt tcatagacga
     6061 tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag agaaacacaa gctatttcct
     6121 gctgattgtt gacaacggtc atcccgccac ctttttgaat caaaagatta gcagcataca
     6181 gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg cgagtcatgg gcaatcgttg
     6241 tcgctatcgc tccttgtttc agctgaaatc ctttcactat gcctaatcca atgtttcccg
     6301 tttgatggtg acgctcgatc accgcaatct ttaattggtc tttttcgatt gaaggaatga
     6361 atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt agtcactaga ctattcggga
     6421 taatttcgat cacatgggct tgtgaagaag aaagaagcag ctctaacgaa tgtggattta
     6481 atggttgagc gtttagtttt ggcagacaac acgtatatga atttgaatga tcagcaaata
     6541 aatggtcttg gagttgccca gattgaacaa ctaatgttcc cttatagaaa acttcatgaa
     6601 ttggcagctt ggtaagatcg tctgttaaga agaaatcagc ttgataccca ggggcaatcg
     6661 cacctagtgt tttcaaatca aaacattctg ctgcattcaa tgttgccatc tgaatggctt
     6721 gtaatggatc gattccaagt gcaatagctt gacggacgat atgatcgatt gatccttctt
     6781 ttactaaatc attgatcagt ttatcatcgg tgacaaagaa gcatcttctt gaattttcag
     6841 gtgtgatcgc cggtagtagc gatgccagat cttttgcaac ggtcccttct cgaaccatta
     6901 aatacatacc aagatccaaa cgttctttag cttcttttgc agttgtagct tcatgatccg
     6961 tccgaatacc tgctgcagga taaacattta actcttccat gccgatacca gctgcatgcc
     7021 cgtcaatttt tttcttatgc tggtgcatca attggatctt ttctaggatg tctgtttcat
     7081 tatttgctac tgcttgatag ttcatgactt cagctaagcc taaaacttcg ggtttttgta
     7141 aaaaaggggc taattttttt gcatttaaat ttgcaccatt ggtctcaaaa ggggttacag
     7201 gtacacaaga aggtagcata aaaaagatat tcatgagtgt ttgtcttgca tcttctatca
     7261 taaactcaag acctgtactc ccagcaatgt ttcctatttc atggggatct gtcactacag
     7321 tagtaactcc atgaagcaag gatacttttg aaaattcgct tggtgataaa agtgagcttt
     7381 cgatatgcac atggccatcg atgaatcctg gcactagata ttgcccatta gcattatgta
     7441 cgctttttcc ttcgtattca ccgattccta cgatcattcc ttcgcagatt gctacatctt
     7501 tttccatgat ttctttcgta aagacgttga cgatcttagc atgcttgatc actaggtcag
     7561 caggcttttt tttacttgct acttcgatat atttccttaa tgttgtttcc aatggagtgc
     7621 ctcccaagat aaatttttat aagtataaca taaattcggt ttttgccaat atttttttgt
     7681 aaattcattt tatttgaaac aaaaagatca aaaagacgta tgttttttgt ggaggggaga
     7741 ataatggaaa taaaacgatt atcggatcaa atgataagtg aaagttttgc caaacatgcc
     7801 caagacgtat atatattgcc agcttcagaa gtattttaca tctattttcg ctatggaaaa
     7861 aaacgaacat tctggaagaa agtctcttcg gcagatggac agcaactgat ctcaagattt
     7921 aagtatttgg gacagatgga tatcggagaa aaaagaaaag ctcagttagg tgcatttact
     7981 tattctttag aaaaacagga agttcgtctg cggttgtctt ctgtaggaga ttaccttcaa
     8041 cgagagagtt tagtgattcg gctattatac ggtacttttg aaacacccca tcgttgcttt
     8101 agttctcgag attttgcaac tattttacag aaaaccgtca gacgtggatt ataccttttc
     8161 agcggaccag tcggatcagg caagacttct ctaatgtatc ggttggcttt ggaagaaaaa
     8221 ttgcaagtga ttactatcga agatcctgta gagatagaag aaccgttttt tctgcagcta
     8281 cagatcaatc cgaaaatagc acagacttat gatacacttt tgagactcgc attacggcat
     8341 cgtccagact tgctaatcat tggcgagata agagatgaat caactgcaca tgctgcggta
     8401 agagcagcac taacagggca ccgggttttt gcaaccgtac atgcacgcaa tcttgaaggt
     8461 acattggtca gaatccatga actgacaaag aaaaaagaag agctatctga atgtttagct
     8521 ggtgtcattt atcaagaact tttggaagat gccaatcacc atccttctgt tttatggagt
     8581 tatcaattta tggaggaaga atcagtaaaa aaatcatggg aggaaagtta tgaagaagct
     8641 caggaaaata tttggcagtc agaactattc taaaggatta acaagaagcc agcaaaacca
     8701 gtttgcacaa accatgggag aactgctttt atctggtttt agtctgcaag aatcccttta
     8761 tttattactc acgatccgtt cgtttccacc tgttattttg gcaaatatcc aacatcagct
     8821 aaaggaaggg caagcgttta gtgaggtact ttcgcaaata ggatatggag cagagcaact
     8881 ggtacagatc gagttagctg aacgtcatgg gaatctgccg acaactttgt tggaaattgc
     8941 caaacagttt cggcttgttg aaggatttcg caatgatttg aaaaagctgc ttgcatatcc
     9001 gatgctattg ctcacctttt tgctaggtat cttgtttagt atgcggttgt tcgttttgcc
     9061 tcagcttatt ggttcaggaa tggtcatgca agagcattgg ggtattcagc tgatacaagt
     9121 ctttcattgg tatgttctac tattcatcct agcggttact ctattggctt tactagtaag
     9181 gtatcgtctg atgaagttgt ctcctgttga tcagagtgaa tggataagca gacaattatt
     9241 gattggaccg atatattcct tgtatcaatc ggcttatttg tcactagaga ttggaaagct
     9301 ttttcatgaa ggtttcgaat taaggcaaat catagtctgc ttaaaggaaa cgaaacaaga
     9361 aagtctgatt cagcatcttg cctctaagtt gattcaagga ttggaggcag gtatgtcatt
     9421 ggctgagcag ttccagaatt atcgttttct atcagaagaa ttcagtaaaa ttgttttaca
     9481 gggagaggca aagggaagtt taggtaaaga actgattttt tacagcgaat ggacaagaaa
     9541 acagcttttc aaacgtcttc atcactggat gcattttatt cagccaatca tttttttggg
     9601 cgtagcggct ttgattttat tagtttatgc cgctgtttta ttacctgttt acggaagcat
     9661 agaggaggtt ttgccatgaa aaagagaaaa atatcttatt atagcggttt tacattgatc
     9721 gaaatgctaa tcgtcttact gatcatcagt gtattagtgt tgttgttcgt ccctaatttg
     9781 tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag cgatcatcca gttggtagac
     9841 acacaaaaag agctctatgc gcttcaaaat aatggaagag ttcctactgt ggaggaattg
     9901 ttgaatgaag gctatatcaa acgagaacat gcagaaatct atcagcggcc ttgaaggtaa
     9961 ggttttagca tcaggatata cgttgtttga atcactctta ctgattttat tacttacaac
    10021 gatcctttct tttccagttc tcgcattttc tgcatggaag aatgaattgg cagtctatca
    10081 atttttcggc caatttgaaa aacgaatcta ttctacgcaa aaaatcgcta ttgtgaatca
    10141 gatccaaaca ggcttttatt ggaatagtga agagaatcaa atcatttttc atgttccgaa
    10201 tccagataaa ataaattggg aagtattaga tattcctgat gagattacct tgaaacgcca
    10261 tgcttctatt acttttgctg ctagaaccgg gaatgaaagt tcactaaaag cttatcaatt
    10321 ttattgggaa gaaaaagagc ggacgattac gtatcaattc cagataggga gtggtcgcta
    10381 tacaaaaaga attgaataaa acaggatttg ttttgttgga gtcccttaca gcgcttatga
    10441 tcgcttctgt ctgtgtctac ttagtgagct ttggccagct gtttttgctt caggcagaaa
    10501 agaaaagtgg ggaggaactg ctatttattc gaatgattta tgaagatatc aagacgcagc
    10561 ggatgtatca caaaagacca gagcgaaggg aagaaatgat cccatacgat atccaaattg
    10621 tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat ggagaaagaa gcgatcagta
    10681 ttgaaaaaac aagtaattcc agcatttacg ctgattgaat gtctaattgg actgatgata
    10741 ttgagcagtc tattgctcac attcaatctt ttgattcagc aaaccatcca agtaagtgaa
    10801 tttttgcagc gtaaagatca aaaagagtgg ctaatatttt tagcacaatt agaagaagaa
    10861 ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac tgcactacac gatcgaggat
    10921 aaacagtata tcatcgaaag atatcaggca atgatacgta aaagaagaac ttccggcgga
    10981 catcaaccta tgttaacaag tatcactgag cttcaattat tggaaaaaga acacacaatt
    11041 tctttttatg tacactttga aaatggagaa gatggatatg ccatatggac aaaaaacgat
    11101 cagtagaaaa tgttataagg gctctatttt attttctgtc ttgcttttat ttcttttatg
    11161 tacgttttta ttgatggcag cattagaaag ctaccgtttg tcaatggatc ttaatatacg
    11221 aatcaaaaat tattatatag caaaaataat gaagcagatg gctttatcgg aacagtctac
    11281 gcttgccgaa agttcggaag gagtttttta ctacacaact ggatctgtaa catatcagtg
    11341 ggtacaacag tccctattct tagaggtgga agtctcaccg tttatctttc ggttcacaga
    11401 agaagtaaaa aatgatacag atactagtac agaatgatgg aatgttcgtg aaaacgccct
    11461 cttttctgtt ataacactgt ttttaataaa aaaatgtttg ccgtttcaca aataaaaaac
    11521 agtgaaaacg attgaaattt tgtcaattgt tcgatagaat taaacaggtg aaaaaattgt
    11581 tataatatat ctagaaatga ggtgcaccat gttaccagaa aaaatggaac aagggtttaa
    11641 tcaaaacctg gaagctattc agcttttaca gaatgcattg ggaacttcct ttttagaagc
    11701 ctatgtcgaa aatgcagaaa atttaataga tgattatcaa gtacgtgtgg tggatggagt
    11761 gccgacaaaa gagacaacgc aacggatcac agctctttat gaagagttga agaaactttc
    11821 tttcgagcca gaagaatggc gcagactgtc tcaattactt ttattgaagg gcagccagac
    11881 agaacatttg caggcgaacc atcaactcac tcctgatagt atcggttttt tatttgtttt
    11941 tcttattgaa caactataca caaataaaaa agcaccggtt aagattctgg atattgcggc
    12001 aggaatgggc aacttgttat taacagttct tttaaaccta tcgaatgcgg ggtaccaaac
    12061 agaaggaatt ggtgtggata ttgatgatac attgttggct gtcgcagctt ctacaagtga
    12121 tttgacacaa gcaaatgttc aatactttca tcaagacggg ttgcaggaat tattaataga
    12181 tcctgtcgat tttgcaatca gcgatttgcc aatcggttat tatccgaatg acgaaaaagc
    12241 aaaagagttt ttaacaagta ccgaagaagg ccatagttat gcacatcatt tattgctaga
    12301 gcaatcaatg aaatatgtga agccggatgg ttttggtttg tttttgatgc cgagtggatt
    12361 tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt aaagaagaag gttatcttca
    12421 aggaatgatt caattgccag atgaattgtt ccgtaataaa caatcgcaaa aaagtatttt
    12481 gatcttacag aaaaaaggac cacaagcaca acaagtaaaa gaagtcttgc ttgtgaaact
    12541 tgcttcatta aaagaaccag agaaggtgac ggaatttttc aacgagttta aaaactggaa
    12601 atcatctgct ctgtaatcat tcaacgagaa ggagagtcat atgtctaaaa caattgcaat
    12661 caatgctggg agttcaagtt tgaaatggca attatatcaa atgccagaag aaaccgtagt
    12721 tgcgaaaggg atcgtcgaac gtatcggttt acaagattct atttttacca tcaagtacgg
    12781 cgataatcaa aaattcgaac aaatcatgga tatcgaaaac cacgaagtag cagtaaaaat
    12841 gctgttagat cagttgattg aactgcaaat tttaagctct tatgatgaaa tcaccggagt
    12901 agggcaccgt gttgttcacg gaggagaaac ttatggggat tcagtagtga tcgatgagaa
    12961 agtaatggac cgcattgctg aattagcaga atttgctcct ttgcataatc ctgcaaactt
    13021 aatggggatc aaagcgttta aaaagatttt acctgacatt ttgagtgtgg ctgtattcga
    13081 tacatctttc cactcaacaa tgccaaagca aaattattta tatagcttac cggtgtctta
    13141 ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc acaagtcatc gttatgtgtc
    13201 ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa ctaaaaatca tcacttgcca
    13261 cttaggaaat ggtgtatcga tcacagcagt cgatggaggt aaatccatgg atacttctat
    13321 gggattcaca ccattagctg gggtgacaat gggtacacgt tcaggtgata tcgatccagc
    13381 tgttctccca tatttaatgg aaaaattaga gattgatatt gatgaaatga tcaatgtttt
    13441 gaacaagaaa tctggtctgc taggcctaac aggattgtct agtgatatgc gtgatcttga
    13501 aaagaactat gaaaaagaac atattcgttt agcttatgac attttcgtgg accgcatccg
    13561 aaaatacatc ggcggttatg tgacaacgat gaatggtgtt gatgcaattg tctttactgc
    13621 tggtatcggc gagaatgacg gacatgtacg tagtgaaatc atcaaaggta tgtcttggtt
    13681 tggttgtgaa atcgatccag cgttgaatga attgcgtggt gaagaagtag atatctctac
    13741 aaaagaatca aaagtaagag tattagtgat cccaacagat gaggaactaa tgattgcccg
    13801 tgatgtggag cgtctgcgcg gctaagccaa agcattcgtt aggataaaag atgaaacgca
    13861 tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta
    13921 acttcaattt cccttctttt ttacaggatc ttcttaggga attttgcttg gaaaagtggt
    13981 caaaagggaa aagactggca ttttatttat tgattttcag gaaaatccca atattttatt
    14041 ttcattcatc tttataaggg acattacata tgtaaaaaga aaaaacattt ttagatcagc
    14101 ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca
    14161 ttcattgatc gaataataaa aacttgattt tccacctgta ctttgcctga atcgtgaaag
    14221 cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat cagttggccg
    14281 agcggctggc ggctgatctg ctcctgatca ataacgagtg cttcttggaa gtgttgctgc
    14341 aaaaagtagc ggtcatggct gactgctagg agcgttcctt gatagtcttg cagcatttct
    14401 tcaatttctt ctcgagcata gctgtcgagg tgattggttg gttcatccaa aatcaggacg
    14461 ttgatttttt ctaaaaagag tttcagcaac gccaaacgga ttttttcacc accggataaa
    14521 tctttgatcc gtttacccac atcgctttga taaaagccac ttttggctaa ttgccgtcga
    14581 gcgacttctt cgtttccagt caatgtgatg gcgtaagcca gcaaacggct atcgggatcg
    14641 gcaaactgca gctgctgcgg caaataaccg atttgcaagc tactacctaa tttgatggtg
    14701 ccaccgtaag gcttttcctc accgagtaat aatttgagta aggttgattt tcctgcacca
    14761 ttgtcgccga tgatggctaa atgatctcca cgatagactg caaaactgct gtctttgaat
    14821 agtacttgat cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc tttgccagat
    14881 tttccaccat tagacaagct ttgcagccga tttttcgggg gaggtggcgg tttgacgagt
    14941 gtcagtttcg ccaggcggcg ttccagttct ttggcttttt tgaagaagga ctcattgtct
    15001 ccttcatgtg cccactgacg aaaacgacga atcatgactt tcaaccgctg gacttctttt
    15061 tgctgcagtt cgtaattttt tgtcagctca gccaagcgag cttctttcaa gactcgaaaa
    15121 cggctgtagt ttccgggata ctcaatcaat gcaccttctt ccagttccca tgtttttgtg
    15181 gtgacttgat cgagaaattg ccgatcatga gaaacgacca gaaccgattt ttttgtattc
    15241 tgtaaatagt tttccaacca gcgaatactg ttaagatcta aatgattggt cggttcatcg
    15301 agtaaaagta aatcggcatc ggctgctaaa attttggcta aagcgacttt aactcgttcg
    15361 cctccgctaa gttctgttaa ccgtgcttcc tgacggtgtg gaatccctaa gccttgcaag
    15421 atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc
    15481 tcaccgtaac gggccagaca cttctctaag tttgtatctg gttctgtcat caatgcttcg
    15541 tagcgactca tttgctgttt gaggtcctgt atctcagcgg cagaatggag caagtagtgg
    15601 ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg tcccgatctt taagcctttt
    15661 ttgcgactga taacaccgct gtcgacacct tctctgccgg tcaagatttg taaaaaggtt
    15721 gatttgccag ttccgttgat accgactaag gcaatttttt caccagcttc aatttgacag
    15781 gataaatcct caaataatgg attcccgccg attgtttttt taatagaaat tgcttgtatg
    15841 atcatgatct ctctccttga gaccactcaa aaaggccatg gttatccatg gcctgtgtta
    15901 tttaaactca gattatcatg agaaaaatac cttgagatgg ccattgattt tacgtgaatt
    15961 caactgtttt ggacaaactt gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa
    16021 atgcgatatg gtatcgttgg taattttcat tacttgttat tttagtcatg cgcatcatag
    16081 tattttccct ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca
    16141 actagcgctg acatttactt aatatttatc gatgtgtaat tctgtcagag agcttttttt
    16201 atctgactga gggtaatgtc tcgtggacca catcattgta ttctgagcta aaccagcgtt
    16261 caggcgaaat catatggtgt ttgataaaaa actttgtcta catgacttcc ttttgccatt
    16321 tgcttaatac cgttgcatta aacagctctt ctctttggaa atctcaccaa catcgcgttc
    16381 aggagtatcg ttgtggttgc aatggcaagc ggtagccttt catcaattct tgaatgattt
    16441 accgaatgta gcacttgcct tctcgcggta cccgaacccg aaataaaatt ttagtttggc
    16501 gataaaatcg gtatccgcca tcgatacttc atattttttc gtatcagatt catcggaaac
    16561 ttccacattg acgaaccaat aagctttggc atgcttaggt cgttttaaac cacaccaggg
    16621 cgactaatcg ccctggtgtt cacttttttt tattttactt gaactcattg actgttttcc
    16681 ctttatttta aacattataa agaccgatga gtgcttgccg cgtcttttgt ttttatcacg
    16741 gttttttgtg caaaaaaacc tgccgatagt tggcaggtcg tctacgtgtt aagctttggt
    16801 gtggtcttta gctggcaatt ggttttctaa atcagttcgg acaacaagca catcgcaagc
    16861 tgcgtttcta ataacatatt cagaaacaga accaataaat aaacgttcta cagcattcaa
    16921 tcctgtggct cctaacataa tcaaatctac ttgattatct tctgggattt ctctggcaat
    16981 aatttgtttt ggtgagccat attctacgac agaggttaca ttattcaagc cagctttctt
    17041 cgcattgctt tcataatctg ccaatgtttg ctttgccatt tcagtagctt gttctgcaag
    17101 cattccatca aaggatgaaa cggtttggaa agaacgtgtg tcaatgacat gtgctaatag
    17161 aagttctccg ttatttcgga tagctacatt gaccgccttt ttaaaagcta attcagcttc
    17221 atcagaacca tctacggcaa ccatgatttt tttgtattgt tgtaacataa gcattcactc
    17281 ctcaagtcaa tcgtattgta ctcttttatt gtagaacact ttagggaaaa agagaattat
    17341 ttactgcttt ggttcagcaa ttttttttgc aaatcggata ctgaaggtgg cagaaacaat
    17401 aattacgatc agtaaaaaga ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat
    17461 acccattacg cctaaaacag catatgcagc tccgtaaaat tgtaggaatc cttgattttt
    17521 atcatttttg ttgatcaata caaaaaagat atcctgttta ttaaataaat aatagctaac
    17581 gaataataat actaaagtgg cgataatcat taaaatccta atcatgaaca aatcctccaa
    17641 aataagcaac aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga
    17701 tggtaatctc cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag
    17761 gtgggtccca tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac
    17821 acaagacggg tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc
    17881 gaacatcaca gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga
    17941 gctcgcagaa acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt
    18001 ttatacggta atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat
    18061 aatactgagc attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa
    18121 aatgatgagg atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc
    18181 tatctctcaa atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg
    18241 catctaatgc catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt
    18301 ctgctagtgg gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca
    18361 ctgtacgagc cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca
    18421 gacgccggca aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg
    18481 cagcatccac atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag
    18541 catctccatt tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca
    18601 gtgtaagatg aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc
    18661 cattcaatgc actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt
    18721 cgtctaactg gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga
    18781 ctgccaactg gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc
    18841 ggatcgctgg attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc
    18901 ggccactttc taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt
    18961 catccaataa gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat
    19021 ccttttttga atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga
    19081 tcgcacttgc gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga
    19141 gcatgcgagc atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac
    19201 caacgacttc atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat
    19261 tttcctcctt cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa
    19321 gaacagcctt ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag
    19381 taggtgagaa aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg
    19441 ccagaagtgg cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga
    19501 ttagctggat atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat
    19561 agctatggtt taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc
    19621 tacctttaca cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt
    19681 cctcaaggag taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa
    19741 aatcgtggcg gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca
    19801 ttagcaattc ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt
    19861 tttgagaaaa aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac
    19921 aaaggtgtat ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct
    19981 ttacaaagaa aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa
    20041 ggatctatta acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc
    20101 tttagattgg gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa
    20161 taaagcacaa tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga
    20221 atttgaagac ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta
    20281 tctagcagta atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt
    20341 agtggaatat ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga
    20401 tgaagacgat gacgaagctg tattcgagct tcactgggat aatcagcaac tagcagaatt
    20461 agtagaagta aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg
    20521 agtgacagat atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc
    20581 agttgctaat attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga
    20641 tgttgaacat tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc
    20701 gctgaaagaa ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat
    20761 cttgcctttt atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa
    20821 aaataaagta acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata
    20881 ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca
    20941 gacagaagat cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg
    21001 cactcatccc acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga
    21061 aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg
    21121 tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa
    21181 tatggatatg aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg
    21241 aatcgagatt gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat
    21301 gatcccagat gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat
    21361 tgaagagaag aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca
    21421 aattttccag caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat
    21481 gcagcggtat tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga
    21541 aaactatcac cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc
    21601 attcaatgat caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta
    21661 tctgaaagaa attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc
    21721 gagtggctat cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg
    21781 agcgcaagct tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa
    21841 acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca
    21901 tcggcagatc gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct
    21961 agatgattat gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc
    22021 aaatgcagcc aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg
    22081 accagaaggt ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact
    22141 ttgtggatta ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc
    22201 tgctagctat caatgggaat taatgaatcc ttctttcagc taacgttctt cattaataaa
    22261 agagtatcga cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat
    22321 ttatacaatg gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa
    22381 aaaaacgtat aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc
    22441 cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg
    22501 gacccggtgt cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg
    22561 agaaagcatg cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac
    22621 cctacattat tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc
    22681 atacagtggc tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag
    22741 atctgagaga agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag
    22801 ggaaaataaa atataaatcc cacgaagagc agctagcaga aaaccaccga aagatgctgc
    22861 tagcaatggc tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca
    22921 tgcgtacgtt gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag
    22981 aaatctatgc gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag
    23041 atacagcact tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga
    23101 cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga
    23161 cagaagagct ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct
    23221 atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc
    23281 gagtgatcgt agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat
    23341 ggaaaccaat gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc
    23401 aatctttgca tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa
    23461 cacaggaaat gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag
    23521 gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga
    23581 tactcgaact tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag
    23641 atatctttag tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa
    23701 aaggatccgg accattggac tttgcataca gtatccatac agatatcggt aacaaaacca
    23761 ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata
    23821 tcattgagat catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag
    23881 ttgctactag caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag
    23941 aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc
    24001 ctaaagatat tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa
    24061 cagaagatga tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca
    24121 atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag
    24181 aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg
    24241 gcgttgtcat tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga
    24301 ttcctggtga tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc
    24361 gagattgtcc gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg
    24421 aatgggaaga tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct
    24481 ataatcgttc aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc
    24541 tcaacagcgt tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg
    24601 gtatccagaa tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg
    24661 tctacagtgt acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg
    24721 gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta
    24781 cttgttttat taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa
    24841 aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa
    24901 gatgtgggag gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa
    24961 ggaaatcgac caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa
    25021 tcattcaatg aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca
    25081 gatatgtcgg tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac
    25141 aaataaagta atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact
    25201 gataaactct aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc
    25261 ggaaaaaaag ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga
    25321 aagaagtacc agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat
    25381 acaacatgtt ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt
    25441 tcaataaaga agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa
    25501 ggaatttcaa acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag
    25561 gtggagtaca gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc
    25621 taatgacacc ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag
    25681 cacttgaagc acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta
    25741 catctatttg cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa
    25801 aattagcaga atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca
    25861 taaaaggtgt cgagaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag
    25921 ctcgacaaaa tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca
    25981 cagatttaga tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca
    26041 tccgtccaac ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta
    26101 gaccagattt gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc
    26161 aagaaatgag acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag
    26221 tagaagtaga tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa
    26281 cagaccatga tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag
    26341 ggaaagcaaa agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa
    26401 attttcgaca aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt
    26461 tttccgatca gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc
    26521 cattcaatca gtttccgact tatccgaata gccgagcgct actttacaag aattagatta
    26581 gcataaactt atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta
    26641 ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata
    26701 atcttcattg gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg
    26761 cttagctcca ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag
    26821 ttgcgcagga gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa
    26881 aatttctctg aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc
    26941 aaattgtttt tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa
    27001 atagggaaca aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg
    27061 ccgatggatc aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc
    27121 tttcaattgt tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt
    27181 taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat
    27241 ataaaaaatg tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt
    27301 gagaaactca ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt
    27361 agcgatgacg acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc
    27421 ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa
    27481 agctgtacga actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc
    27541 tccaacggca tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct
    27601 tatttctgcc atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct
    27661 gaactaaaac gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt
    27721 ttagtaggtt ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt
    27781 acaagagagg atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct
    27841 ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa
    27901 atctttcaaa attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca
    27961 agggtgattt ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga
    28021 ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa
    28081 acagcacgtt tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa
    28141 cactatgaag tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg
    28201 tatgattttt atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca
    28261 atcgttcgtt cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa
    28321 actttttata tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa
    28381 ttccatcaaa tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc
    28441 atggtgatgg cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc
    28501 aattcattag gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt
    28561 ttaccttttg aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta
    28621 cgcgtattgg acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc
    28681 ttagattatc taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat
    28741 gcattggctg ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca
    28801 catacgattt ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc
    28861 gcaggcggac gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc
    28921 ggatttgcaa tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc
    28981 cctgcattta atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa
    29041 gcattgaaag tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg
    29101 aaccgtaaag caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg
    29161 gtgatcggtg aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga
    29221 aaagaaaaaa gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa
    29281 atgactttat tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat
    29341 tagtttccga acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac
    29401 gagaccttgg gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg
    29461 tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat
    29521 acgtgatcga agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa
    29581 tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa
    29641 ccccgccatt tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc
    29701 gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa
    29761 caaaagcgat tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc
    29821 tagggaaatc tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg
    29881 gacatttcta tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg
    29941 gattcgaccg ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc
    30001 gtcagcctga atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc
    30061 aaacttacac agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca
    30121 cattgccatt tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac
    30181 cggatacacg ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat
    30241 tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag
    30301 gcgctgcaga tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat
    30361 tcggagctaa ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg
    30421 ctaaattcat gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg
    30481 acttgctgat gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc
    30541 ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg
    30601 taactgattg gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc
    30661 atccattcac gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag
    30721 tctatgcaga agcttatgat atcgtcttga acggttatga attaggcgga ggttctatcc
    30781 gtattcatac tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac
    30841 aaatggaaga acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg
    30901 gcggtattgc attaggattg gaccgttttg caatgttatt agccggagaa gataatattc
    30961 gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa
    31021 gtatggtttc gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat
    31081 agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa
    31141 ttgaaaaaca ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa
    31201 cattcgaaaa ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg
    31261 cgttgatcgc atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg
    31321 gtattactgg tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag
    31381 taccggtatc aacaactttg tatttactga atataccttt gtttgtattg gcgtggttaa
    31441 aaatcgggaa gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta
    31501 tccatatttt accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg
    31561 gggctgtgat gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc
    31621 tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga
    31681 tctacttcaa tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt
    31741 tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac
    31801 aaaagaagat gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc
    31861 aaaactgtat gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg
    31921 ataagcagac agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag
    31981 caatgaaagc ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg
    32041 gtcgtttcta tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg
    32101 tagacagacg ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa
    32161 ctgctttttt atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt
    32221 tatgggatga tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt
    32281 aatacccgta tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa
    32341 caaaaaaggt taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct
    32401 gtggtgggtg ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc
    32461 gttccgattt ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg
    32521 gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat
    32581 ccggcacttg tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg
    32641 ctgttaaaca atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg
    32701 caaatcaata acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat
    32761 atctggtctt tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg
    32821 ttgatggtat ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc
    32881 ctctccgcca tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat
    32941 tacggtaccg acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg
    33001 ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct
    33061 ggaggaatct cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat
    33121 ttcatctatt acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg
    33181 acagcacacc ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt
    33241 ggcaaacgaa gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca
    33301 aaggtgactg atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt
    33361 ctaatcatca gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat
    33421 ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa
    33481 ccatgggttg ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca
    33541 ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa
    33601 aaattagcag aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg
    33661 atccttgata agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta
    33721 acggcatttg gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca
    33781 aaagtagtgg tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa
    33841 aaattaaagg aacaagattt gttgaaagac actgttctga ttggactagg aacgaatggc
    33901 tcattctcgg aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac
    33961 tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa
    34021 cagatgacga aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac
    34081 catagtgact ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac
    34141 attcacttag tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca
    34201 aacgtgacag aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag
    34261 tttttttata tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag
    34321 tgtgtctgta aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct
    34381 tttgttgctt cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt
    34441 ttttgaagac gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa
    34501 agatcggttg tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa
    34561 tgttcatttg ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta
    34621 agtgatcaat ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac
    34681 taacaaaata tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc
    34741 aattcgatag tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat
    34801 agtaaagcgt ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt
    34861 cgtatgaatc tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg
    34921 cagccctttg aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac
    34981 agataagctg tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag
    35041 cgcgatacta cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc
    35101 cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac
    35161 aatcttccct tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa
    35221 agaaaacact ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg
    35281 ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac
    35341 tatttttatt aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg
    35401 accataagaa gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag
    35461 tactattagc cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag
    35521 atgagaatta catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg
    35581 tagctgcagc acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag
    35641 ttctgcgatt tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac
    35701 caagcgtcat taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt
    35761 ttcccagtgg gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa
    35821 tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt
    35881 tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga
    35941 aaacggataa agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat
    36001 tggacaaaga agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt
    36061 ttatgcgtgg gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa
    36121 acgagggaga gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca
    36181 ggtgtcatgt aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg
    36241 aatgcgtttt ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt
    36301 gattgaagac cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt
    36361 agaggcaatc gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt
    36421 agaattatac aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc
    36481 tatatcagga aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta
    36541 ttcgaactat tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa
    36601 cgtacaattt ttctttcttc cttactttga accaattgca gcgagattat attttgaaga
    36661 tgacaatatc cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct
    36721 atttgattca gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa
    36781 atctgattcg gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact
    36841 tacaccgttt gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga
    36901 taatgctcgc tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa
    36961 aggcgtatgg atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc
    37021 cctgcatgat attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta
    37081 tcagtccgtc aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc
    37141 gaatatgatg aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa
    37201 cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa
    37261 attagcagaa gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa
    37321 atggctcgga gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa
    37381 aaaattacaa cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt
    37441 gacagaagca ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt
    37501 tgatgggatc accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga
    37561 gatgcgttct ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca
    37621 tcagcagatg agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg
    37681 aaacggaaca aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa
    37741 agaaataaaa caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa
    37801 tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt
    37861 tttggtttca tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt
    37921 tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga
    37981 tcatttagag caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga
    38041 accagcagca gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga
    38101 aaaacaaatc acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact
    38161 agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt
    38221 aataaacaag caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg
    38281 gattgggtat tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat
    38341 aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat
    38401 gactacgcag aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac
    38461 aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga
    38521 atccttacgt agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga
    38581 attgaatgcc gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct
    38641 tcaagaaaat ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt
    38701 aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc
    38761 gctcgcacag ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca
    38821 gctattgaaa gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca
    38881 aattgctcgt cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac
    38941 ggaacacccc atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt
    39001 agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa
    39061 aagtgaagca catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat
    39121 ggcaacgaat atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc
    39181 atttcctgag ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt
    39241 agatagacga aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca
    39301 agcagaaaag ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa
    39361 tcagctccag caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga
    39421 tagtaactgt ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa
    39481 tcaactagct gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg
    39541 gacacagctt gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga
    39601 acaagaaaga atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat
    39661 ctgtgcattt gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta
    39721 tcatgaagag caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac
    39781 tgagacattc ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat
    39841 cgaaataaag caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat
    39901 ctacgatgaa tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca
    39961 attacagcag ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg
    40021 gtatgtgctt caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg
    40081 tttaacgaaa aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa
    40141 aaccggatta gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac
    40201 cctgtctggc ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt
    40261 gatccagaca caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg
    40321 gtcattagat gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga
    40381 aggaagaatg atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca
    40441 aataagaatt gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa
    40501 agggagattg gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca
    40561 atacttgcaa gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca
    40621 tgcagaagtc ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat
    40681 agattactgc caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt
    40741 ggaactttat ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct
    40801 agaaccggta aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa
    40861 aacacaaaac aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat
    40921 tgagactaat cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc
    40981 attggcatca ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta
    41041 cacagaaaaa gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc
    41101 gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca
    41161 attattaggt acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct
    41221 cccagtagaa caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct
    41281 gcagataaat gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc
    41341 attcaaagtg gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt
    41401 tttctttatg cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata
    41461 tcaacaaacg gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt
    41521 agtgttcaag aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga
    41581 gatcggcgac gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga
    41641 atttattttc tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta
    41701 tggtgaagtc atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca
    41761 ggtgttacgt gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta
    41821 ccagaaaaat gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt
    41881 tttccgcaca gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt
    41941 gcgagagttg tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg
    42001 ttcatggctc gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt
    42061 cctgcaaagc ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc
    42121 ttttgattca gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat
    42181 acgtacagaa gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca
    42241 attggaaaaa agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat
    42301 tcatccagaa agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta
    42361 tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt
    42421 agccgatgaa atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa
    42481 agaagaacgg aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg
    42541 gcaggcagag gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa
    42601 aaaagaacgt gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag
    42661 cttaagacag gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga
    42721 ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt
    42781 acctcagcgg tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc
    42841 attgtttgca acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc
    42901 cgcagaagaa attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt
    42961 gttgaaagac ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca
    43021 aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc
    43081 tgaagcattc aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat
    43141 ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca
    43201 agtaaaagat ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca
    43261 atttacaggg atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt
    43321 ttatttaaga ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc
    43381 tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac
    43441 gggagcagat acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc
    43501 tgctggacgt gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc
    43561 tgaaggaacg attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca
    43621 aaaagtgatc caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat
    43681 gattttaagt gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt
    43741 aagaaagcct ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg
    43801 gattccattt tatcccagct tccttgctat cattttttta tttctatttt taaaggttat
    43861 acaaagattg tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa
    43921 attggagtct gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca
    43981 ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga
    44041 agcatatgaa attctttctc agccagctga aaaatctctt tttctggttt ttggaaacct
    44101 gttgcttgag aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc
    44161 agtttttttg tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt
    44221 tccttcaaga aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct
    44281 tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa
    44341 tcttttagtg attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc
    44401 attacttttg ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata
    44461 tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga
    44521 tcatacatcg tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt
    44581 cggttttcat tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa
    44641 aagctgataa ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt
    44701 tatatttcag tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat
    44761 taatcattat ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcgaa
    44821 agatttattt ttttaataca gtctcaatat aactattcaa aca
//
LOCUS       DAYYKN010000008        44565 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000008 DAYYKN010000000
VERSION     DAYYKN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.008"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..744
                     /locus_tag="ACHBB7_000352"
     CDS             97..744
                     /locus_tag="ACHBB7_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM9208335.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            759..2285
                     /gene="zwf"
                     /locus_tag="ACHBB7_000353"
     CDS             759..2285
                     /gene="zwf"
                     /locus_tag="ACHBB7_000353"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM9208336.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2334..5132)
                     /gene="ileS"
                     /locus_tag="ACHBB7_000354"
     CDS             complement(2334..5132)
                     /gene="ileS"
                     /locus_tag="ACHBB7_000354"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM9208337.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5394..6101)
                     /locus_tag="ACHBB7_000355"
     CDS             complement(5394..6101)
                     /locus_tag="ACHBB7_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM9208338.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6122..6904)
                     /locus_tag="ACHBB7_000356"
     CDS             complement(6122..6904)
                     /locus_tag="ACHBB7_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM9208339.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(6953..7174)
                     /locus_tag="ACHBB7_000357"
     CDS             complement(6953..7174)
                     /locus_tag="ACHBB7_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM9208340.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7255..7854)
                     /locus_tag="ACHBB7_000358"
     CDS             complement(7255..7854)
                     /locus_tag="ACHBB7_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM9208341.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7868..8545)
                     /locus_tag="ACHBB7_000359"
     CDS             complement(7868..8545)
                     /locus_tag="ACHBB7_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM9208342.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8562..9803)
                     /gene="ftsZ"
                     /locus_tag="ACHBB7_000360"
     CDS             complement(8562..9803)
                     /gene="ftsZ"
                     /locus_tag="ACHBB7_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM9208343.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9826..11151)
                     /gene="ftsA"
                     /locus_tag="ACHBB7_000361"
     CDS             complement(9826..11151)
                     /gene="ftsA"
                     /locus_tag="ACHBB7_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM9208344.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11307..12527)
                     /locus_tag="ACHBB7_000362"
     CDS             complement(11307..12527)
                     /locus_tag="ACHBB7_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM9208345.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12542..13630)
                     /gene="murG"
                     /locus_tag="ACHBB7_000363"
     CDS             complement(12542..13630)
                     /gene="murG"
                     /locus_tag="ACHBB7_000363"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM9208346.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13656..15017)
                     /gene="murD"
                     /locus_tag="ACHBB7_000364"
     CDS             complement(13656..15017)
                     /gene="murD"
                     /locus_tag="ACHBB7_000364"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM9208347.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15031..15993)
                     /gene="mraY"
                     /locus_tag="ACHBB7_000365"
     CDS             complement(15031..15993)
                     /gene="mraY"
                     /locus_tag="ACHBB7_000365"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM9208348.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16019..18211)
                     /locus_tag="ACHBB7_000366"
     CDS             complement(16019..18211)
                     /locus_tag="ACHBB7_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM9208349.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18212..18613)
                     /gene="ftsL"
                     /locus_tag="ACHBB7_000367"
     CDS             complement(18212..18613)
                     /gene="ftsL"
                     /locus_tag="ACHBB7_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM9208350.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18618..19577)
                     /gene="rsmH"
                     /locus_tag="ACHBB7_000368"
     CDS             complement(18618..19577)
                     /gene="rsmH"
                     /locus_tag="ACHBB7_000368"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM9208351.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19598..20029)
                     /gene="mraZ"
                     /locus_tag="ACHBB7_000369"
     CDS             complement(19598..20029)
                     /gene="mraZ"
                     /locus_tag="ACHBB7_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM9208352.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20199..20567)
                     /locus_tag="ACHBB7_000370"
     CDS             complement(20199..20567)
                     /locus_tag="ACHBB7_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM9208353.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20738..21694
                     /locus_tag="ACHBB7_000371"
     CDS             20738..21694
                     /locus_tag="ACHBB7_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9208354.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21767..21883
                     /locus_tag="ACHBB7_000372"
     CDS             21767..21883
                     /locus_tag="ACHBB7_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM9208355.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21938..23620)
                     /gene="recN"
                     /locus_tag="ACHBB7_000373"
     CDS             complement(21938..23620)
                     /gene="recN"
                     /locus_tag="ACHBB7_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM9208356.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23638..24087)
                     /locus_tag="ACHBB7_000374"
     CDS             complement(23638..24087)
                     /locus_tag="ACHBB7_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9208357.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24194..25012)
                     /locus_tag="ACHBB7_000375"
     CDS             complement(24194..25012)
                     /locus_tag="ACHBB7_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM9208358.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25021..25911)
                     /locus_tag="ACHBB7_000376"
     CDS             complement(25021..25911)
                     /locus_tag="ACHBB7_000376"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM9208359.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25913..26137)
                     /locus_tag="ACHBB7_000377"
     CDS             complement(25913..26137)
                     /locus_tag="ACHBB7_000377"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM9208360.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26142..27479)
                     /gene="xseA"
                     /locus_tag="ACHBB7_000378"
     CDS             complement(26142..27479)
                     /gene="xseA"
                     /locus_tag="ACHBB7_000378"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM9208361.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27480..28334)
                     /locus_tag="ACHBB7_000379"
     CDS             complement(27480..28334)
                     /locus_tag="ACHBB7_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM9208362.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28431..28880)
                     /gene="nusB"
                     /locus_tag="ACHBB7_000380"
     CDS             complement(28431..28880)
                     /gene="nusB"
                     /locus_tag="ACHBB7_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM9208363.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28873..29298)
                     /locus_tag="ACHBB7_000381"
     CDS             complement(28873..29298)
                     /locus_tag="ACHBB7_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9208364.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29544..30608)
                     /locus_tag="ACHBB7_000382"
     CDS             complement(29544..30608)
                     /locus_tag="ACHBB7_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM9208365.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30780..31025)
                     /locus_tag="ACHBB7_000383"
     CDS             complement(30780..31025)
                     /locus_tag="ACHBB7_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9208366.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31164..31538)
                     /locus_tag="ACHBB7_000384"
     CDS             complement(31164..31538)
                     /locus_tag="ACHBB7_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM9208367.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31621..32535)
                     /locus_tag="ACHBB7_000385"
     CDS             complement(31621..32535)
                     /locus_tag="ACHBB7_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9208368.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32556..33359)
                     /locus_tag="ACHBB7_000386"
     CDS             complement(32556..33359)
                     /locus_tag="ACHBB7_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM9208369.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33392..34381)
                     /locus_tag="ACHBB7_000387"
     CDS             complement(33392..34381)
                     /locus_tag="ACHBB7_000387"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM9208370.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34484..34975)
                     /locus_tag="ACHBB7_000388"
     CDS             complement(34484..34975)
                     /locus_tag="ACHBB7_000388"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM9208371.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35166..37973)
                     /locus_tag="ACHBB7_000389"
     CDS             complement(35166..37973)
                     /locus_tag="ACHBB7_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9208372.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(38407..38700)
                     /gene="rpmA"
                     /locus_tag="ACHBB7_000390"
     CDS             complement(38407..38700)
                     /gene="rpmA"
                     /locus_tag="ACHBB7_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM9208373.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(38713..39057)
                     /locus_tag="ACHBB7_000391"
     CDS             complement(38713..39057)
                     /locus_tag="ACHBB7_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM9208374.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(39084..39392)
                     /gene="rplU"
                     /locus_tag="ACHBB7_000392"
     CDS             complement(39084..39392)
                     /gene="rplU"
                     /locus_tag="ACHBB7_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM9208375.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(39412..39488)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            39674..40315
                     /locus_tag="ACHBB7_000393"
     CDS             39674..40315
                     /locus_tag="ACHBB7_000393"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM9208376.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(40377..42008)
                     /locus_tag="ACHBB7_000394"
     CDS             complement(40377..42008)
                     /locus_tag="ACHBB7_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM9208377.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(42131..42490)
                     /locus_tag="ACHBB7_000395"
     CDS             complement(42131..42490)
                     /locus_tag="ACHBB7_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM9208378.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(42649..42722)
                     /locus_tag="ACHBB7_000396"
     tRNA            complement(42649..42722)
                     /locus_tag="ACHBB7_000396"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42686..42688),aa:Arg,seq:tct)
     gene            complement(43031..43714)
                     /locus_tag="ACHBB7_000397"
     CDS             complement(43031..43714)
                     /locus_tag="ACHBB7_000397"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM9208379.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(43842..44360)
                     /locus_tag="ACHBB7_000398"
     CDS             complement(43842..44360)
                     /locus_tag="ACHBB7_000398"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9208380.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
BASE COUNT        12638 a         9650 c         7517 g        14760 t
ORIGIN      
        1 aaaatccttt gttttttatt tagaaaaata gtcaattaac gttttttttg ttacacttac
       61 taatgatggt tttttaccat gaaaggatga tcgaatatga caccaaatcg agaagattat
      121 ttgaaaatca ttttagaact aggtggagat acaacgaaag tcaacaataa acaaatcgta
      181 tctagtctag atgtctcagc tgcatctgtc agtgaaatga tttctaaact tgtgaaagaa
      241 gagcttgtag aacattcccc ttatcaaggt gtacaattga ctgcaagcgg actgcaaaaa
      301 gctagcagtc tgattcgcaa acatcgctta tgggaagttt ttttagttga acatttagac
      361 tatagctgga atgaagtcca tgatgatgct gaagtattgg aacatgtgac ttcggaacat
      421 cttgcagatc atttagaaaa ctatttgaat catcctgaac attgtcctca tggaggatcg
      481 attcctaaaa aagaggaagt tgtccatgaa gaacgccgac aaacattgga gtcctatcct
      541 gtcggtacaa aagtgcgtat cgcacgagtg cttgatgaaa aagaattgtt ggattactta
      601 gtcactattg acctgaatat caacgaagaa tatgagatcg ttgacatcgc agcttatgaa
      661 ggaccgatca ctatccaaaa tgatagaaag aaaatccccg tcagctttaa ggcggccagc
      721 acgatttttg tcgataaaat ataaaggagt gtatccacat gtctaatgaa aaaaatgtat
      781 tgtttacgat attcggcggt acaggtgacc tagcccagcg taaattgtac ccgtctttgt
      841 tccgccttta cagaaaagga aatttaggcg agcattttgc tgtaatcgga accgctcgcc
      901 gtccctggag cgatgagcac taccgtgaag ttgtcaaaga aacaatccgt gcattgaatc
      961 cttcagatga agaagcaact acctttgcaa gccattttta ctatcaatcc catgatgtaa
     1021 atgattcaac gcattatcag acattaaaag aattgtctga ccgattagat ggacactatc
     1081 aattaaacgg taaccgtgtc tattatttgg ctatggcacc tcaatttttt ggaacgatcg
     1141 tttctcattt gaaatcacaa aatattatga caaaaaatgg cttcaatcgt ttgatcatag
     1201 aaaaaccgtt cggttctgat taccagtctg cttttgaatt gaaccagcaa atcagagaag
     1261 tattcccaga acaagatatt ttcaggattg atcattattt aggaaaagaa atgatccaaa
     1321 acatctcagc tatccgtttt gccaataata tttttgaggc tcaatggaac aatcgctata
     1381 tcgacaatat ccaaattact tttggcgaaa acctcggtgt agaagatcgc gggggctact
     1441 acgatcatag tggcgcactt aaagacatgg tgcaaaatca tattttacag gttgtagcat
     1501 tacttgcaat ggaaccgcca gtagcatttt ccgaaaaaga aatccgtaca gaaaagatca
     1561 aagcattgaa agctatccgc atttataacg aagaagaagt gttagaaaac ttcgttcgcg
     1621 ggcaatatgc tcaaggtgaa ttggatggtg tacaattcaa gggctatcgc gaagaagaca
     1681 aagtggatgc tcaatcagag actgaaacat tcgtcgcagg caaattcact atcgataact
     1741 tccgttggtc aggtgttcct ttctatgtac gtacaggcaa acgtttgact gaaaaaggga
     1801 ctcggatcaa catcgtattc aaacaagttc ctgttaatgt attcaaaaca tctgtcgatg
     1861 aaccatgtga cgatacgaca cttccaccta atgttctaac gatctatatt cagccaactg
     1921 aaggattttc tttatccttg aatgggaaag aagtagggca aggatttgaa acggaaccaa
     1981 tcaaattgga atttagaaat agtgcagaaa tggttgaaaa tagcccagaa gcttacgaaa
     2041 aattactatt agatgcgttg aatggtgatg gaacgaactt ctctcattgg gaagaagtcg
     2101 ctcaatcttg gcatatcgtg gatgtcattc gaaaagcttg ggaccaaaca caaccggatt
     2161 ttcccaacta caaagctgga tcaatgggac ctaaagcagc tttcgaacta cttgaaaaag
     2221 acggattcga atggatctgg caaccagata actggtatcg tgaacgtgga aaattaaacg
     2281 attgataaca aaagggggtg tgacaaagaa catgtcacac ccccttttcc cttttacttt
     2341 tcttcgaatc cttcagctac agcttcagga tagaagtctt caacgatttt tgcacaacga
     2401 ccgcatagat gaggaagttt ttcatctact cctacatctt tacgaacttg gcgacagcga
     2461 tcgcaagttt ccccgtctgc tttttccact gtaatagcaa catcatcaaa taccatagcg
     2521 ttatctggca cttgttcatc tgctgccata acttcaaagt agtctggtga aacgatcaat
     2581 aattgtgcaa tatctgcatc caaagctttt aacatttcac gaacttgttg ttttggataa
     2641 acagttagtt ttgcttccaa agatttacca atcaatttgc tgtttcttgc ttcttccaat
     2701 gcttttaaga cattatcacg gaagtccatg aaagcagtcc aggtatccat caattcttct
     2761 tggtttgcaa actcttcata accaggaaac tcgcttaact gaacgtactc ctcttcttct
     2821 ttcaggaatg accagatttc ttctgctgta tgcggaatga taggagtcaa taattttgtc
     2881 aaagctacag ctgtttgata gaaaacagtt tgcatgcaac gacgttgata atcatcttct
     2941 gcttcaatat aaactacatc tttagcaaaa tccaggtaga atgacgatag atcaaccgtc
     3001 aagaagttca taactgtacg gtaaatatgc atgaagttat atttgtcgta cccttcttct
     3061 cggatttctt tgattacttg gttcaatcga acggtcatat atttgtctac agaacgtaaa
     3121 tcattgtacg aaacagtatc ttttttagga tcgaaatctt ctgtattcgc taataagaaa
     3181 cgcatcgtat ttctgatttt acgatatact tctgccactt gacttaagat atccatagaa
     3241 acacgaacat ctgcttcata atctacacta gatacccata agcgaaggat atctgcacca
     3301 aattgtttga tcactttttc tggtgcaatg atgtttccta aagatttact catcttacgg
     3361 ccttcaccat ctaaagcaaa tccttgagaa ataactgatt tgtatggtgc tacaccattg
     3421 atagccacac tagtcgtgat acttgagttg aaccagccac gatattggtc tgatccttct
     3481 aaatacatat ctgctgggaa tgataattct ggtcgctgac gtaaaacagc ttcatgagaa
     3541 gaaccagaat caaaccagac atccatgata tctttttctt ttgtaaattc gccattagga
     3601 gaacctgggt gggtaaatcc ttcaggaaga agttctttcg cttctttttc aaaccagata
     3661 attgatccat gttcagcaaa taattttgcc acatgttcga ttgtttctgg tgtgatgatt
     3721 gcttctccat tttcagcata gaaaatcgga agtggtactc cccatgcacg ctgacgggaa
     3781 attacccaat cgccgcggtc acggatcata ttatacaaac gtgttttacc ccatgggata
     3841 acccactcta ctttttctac ttcatccaaa atattctgac ggaatttatc gatagatgca
     3901 aaccattgtg gagttgcccg atagatcaca ggctttttcg tccgccaatc gtgaggataa
     3961 ctatgagtaa agaaatctag cttcagcaaa gctccttttt cttttaatag atcagtgatc
     4021 ataggatttg ctttgtcata aaagatacct tcaaatccag gtgcttcttc tgtaaacaat
     4081 ccttttgagt ctacaggtga cactacagat agtttatatt tgctaccagc gatatagtca
     4141 tcttccccat gtcctggagc agtatggact aatccagtac cagcgtctaa agtaacatga
     4201 tcgcctaaaa tcacaagtga ttcacgttca tagaatggat gacttgcagt catatattcc
     4261 atttcttgtc cagcaaattc ttttagaact gttacatttt cccaaccgat ttctgattgg
     4321 actgtttcta gcaaatcttt cgctacgaca aacttacggc catcggcttc caccaataca
     4381 tacgtaaatt ttggatttac agcaatacct tggtttgctg gcaatgtcca tggtgttgtc
     4441 gtccaaatga caaaagctgt atcttcatca agaatatctt tgccgtcttt gacattgaaa
     4501 gctacaaaaa tagatggaga ttttacatct ttatattcaa tttcagcttc cgctaaagaa
     4561 gactcacttg acggagacca gtaaattggt ttcaatcctt tgtaaatata gcctttttca
     4621 gccattttac caaatacgcg tacttgtgca gcttcataag aaggatctaa tgtaacataa
     4681 ggatgttccc aatctcctgc aactcctagg cgtttgaaat ctgctcgttg cttatctact
     4741 tgggaaagtg catattcacg acatttttcc aagtattctg ccatggacat ttctttacgt
     4801 ttcacgcctt tattagccaa tacttgttca atcggcaatc catgtgtatc ccatcctgga
     4861 acatatggtg cacgatagcc agacattgat tttgaacgaa tgataatgtc tttactgatt
     4921 ttattcaatg aatgtcctaa atggatatta ccattggcat atggaggtcc atcatgaaga
     4981 acaaagctag gttttccttc gttcaactct tgtctttttt ggtagattcg attttcttcc
     5041 cattcttttt gccactcgcc ttctcggttt ggcaaattac cacgcatagg gaattttgtt
     5101 tttcctaatt gcaaggtttc tttcattttc attcaaccat acactccttt atttgttttc
     5161 cggacacaaa aaagcatgtt cagccgcaag ggacgaacat gccgtggtac cacccaaatt
     5221 tagttgcatc tatctagtgc aaactctctg tgaccaataa cgaggtcagc cgctgcttct
     5281 tactctaatt tcggaaaagc aggtctcaag tagaggatct ttcaccatgc agtgtcttac
     5341 cgaacttcca ctctcatcgg ctcgctttaa gaataacaaa gtgcttttct gttctactcg
     5401 ttttcatttt caccaggcac gatcgagata tcaatagcct gtgtatcaaa tgccgtcata
     5461 ggtgcttcta tgactcctgt gtctgagttt acatcagtat cattttcatt gtcaagctgt
     5521 tttgctaaaa cttctttgat cacttcatgg ctgtcagtca cgtagctgga gaatggttgt
     5581 aaaatttcat cccattccgg acttttcact tgttctaatt gagactcaag catcaagctg
     5641 atacgttgat ggaatactcg tgtttttttc ttaagatctt cagtttctgt cgcaagctgt
     5701 cttgcttttt ctgttgcatc tgtcaagatt tttcttgctt tttcttctgc atcagttgtt
     5761 aattgatgtg cacgtttttc agcgttcgca actaactcat ctgctttgtt ttgtgcagaa
     5821 gtaacgatca cttcggattc cttgcttgca cttgttttta ctttatcagc cgtatcttga
     5881 gctacgatga ttgattggtt caaagcatct tttaattcat tgaagtactc aagtttttct
     5941 tcagaatgtt tgactgcttt ttctaattct cgatttcttt gagtcaattc ctcatagtca
     6001 cgaacaacta aatctaagaa atcatctact tcgtcttgat tatatccccg catttttgtc
     6061 gggaatgtct tattttggat atctaatgga gttaaagcca tgagtaaacc acctttttat
     6121 tttatttacg taatacaccg aacagtaccc tgtacttttc ttttttcgtc ttcccctcaa
     6181 gttcttggat ctggactctg ccataacctc tgactgaaat aatatctaat agttcaagta
     6241 caaaatcagg tcttgtattt tctgtccagt tcacttttac tttaccagct tcgatcagct
     6301 gcttggaccg ctgtctggaa atattataaa tctcagagat cacactgtct agtcgcatgg
     6361 aactaactgt tcctctttct tgtgtccagc tatctttcgg cataatcatt tctgtgtatt
     6421 tgattggttc caagcgtaca gcaacctttc caatctttgt tacttgtgca gcaacaaaat
     6481 gatccacctc atttgcaata aatacttgcc agcgatcacc gtccgagatg atatcgccaa
     6541 agtaagagcg tttgatcccc gttcccacta aagttccaag gattttccca tgacttaggc
     6601 tggcaaattt gctaggataa tggatttcat acaatgctgt ttcaaaatct tcctctacag
     6661 gttcgtaata atcggggaaa atcaaacatc tttttctttc tgcctgttca tatccgccat
     6721 aaaacatgaa acgcaaatca ctgctttgtc gaatcagtgt ttctaaaata taggattgtc
     6781 ttgggtcaag aaattcggtc agatagggtg catattgtcc ttgaacctgt tcaatccaat
     6841 cgcctaccga atcgataaat gtacgttcat ctgcccgaaa gtgctggtat acatttgcat
     6901 ccaaaatttt cccctcctat ctattctgag aataaagatt ttgttgcttc aatcaataaa
     6961 ctaaatggac agttatccat ataacgatct gtgctaaacc ttgtacggct aagttcaaga
     7021 tcaggattgc ccctaaaatt gtgaaatcca agggtccaat cctcaaaggc agacgatcga
     7081 acaaactgat atacggttcg cagatcttag ctaataatcg tcctagccct gattgatacc
     7141 cgcctggaaa ccaagagagc aatgcatata ccatcaagat aatgctatag aaataggctg
     7201 cccgttgaag taaatgaagt aatagtgcaa tagtataaat cgtagttcct cctcttacag
     7261 atcaaacaaa ttcgcatctg caaaagattg agcggttgaa ctgtcaatct ccatcccagt
     7321 cggtgtacaa agaaaaatct catctccaac acgtttgata tctccatcta gtgcataaac
     7381 tgttcctgtt agaaaatcaa cgattcttct cgcttgtttt tcttctacta aatgaaaatt
     7441 cactaaggtt gcttctccag caatgattcg tttagcaatc gccatagctt cagaataagc
     7501 tcttggttcc attacagtga tttttccagc aagatttgct ttaggttgtg tagaggttct
     7561 tgaagctgtt ctttctttcg ctacagtttt tgtaggtgat tgatgtagtt caattacttt
     7621 tttcttttca gccggctgaa tactttcatg cgttacagga cgtgaagtcg ccgttttttg
     7681 tttttcatga gtattcgcag cagatacaga atgagttgat tgattctgtg ttgtattttt
     7741 tttagttggc tgtacagcct tttttgttgt ttcaggataa tcttggtatt cgtactcttc
     7801 ctcatcagct aatccgaaaa aattagcaat tgctcctctg tttaaaagcg gcatttttgt
     7861 tcctccttta ttcgtctttg aatagcgctg ttcctacacg gataaatgtt gcaccttctt
     7921 caatcgcaat ttgaaagtct tgactcatcc ccatacttaa ttcactacat ggtgcatggg
     7981 cataaccttt tgcttgaaca agatcacgca attctttcag cgtaccaaac actttatgga
     8041 tttgctcatc agaagcttct ttaggcgcca tagtcatcaa cccaacaacc ttgatttttg
     8101 tgcaggcagc taaagattcc acaaacggta aaagctcatc tggtgaaatt ccatgttttg
     8161 attcctcacc agaaacattt acttcaataa aacaggataa ttctttctct aaccgcttct
     8221 ggatttcttc tgctaatctt aaactgtcca acgcatgaaa gtaatcaatt tcatttacaa
     8281 tcaattttac ctttctgcgc tgtaaattac caattaaatg ccactttatt tcaggaaaat
     8341 caaataaatc ttgtttcttt tctaataatt tatctactcg gttttctgca caattcataa
     8401 cgccagctct aaccaattct tcagcttgag cggattcaac agattttgtt acagctacta
     8461 atgtaacttc tttcggatca cgatgaacaa gagcacacga atcctgaatt tcttgcttga
     8521 ttttctgcaa gttgtcagca atcatgtgct cttcctctcc tttatttttt acgacggaaa
     8581 aacggtggcg tatttaattc atcatcgcca tgagattttg tttcttcgcg gttgaatgta
     8641 tcaaattctt ttttttcgat attgtcaaaa ttggaatcat caactctagg acgaacattt
     8701 tgttcacgtc tgatatccca atcgccaaaa ctgttttctt cttctgaaga agttggttta
     8761 gcttgatcca tatctaatac agtcttttga gcaggtgatt gcatttgtgc ttgtctagct
     8821 ggtcggctag acttgcgttc cttttttgat tcatcgatac ctgttgcgat gaccgtcaca
     8881 cggatttcat cgcccatttc ttcattgata gatgtaccta gaatgatatt tacatcgcca
     8941 gttgctgcat ttgctacgat atctgaagca tcttgtgctt cgaacaatgt catatccaaa
     9001 ccgcctgtga tgttaagaag tacttgttca gctccatcaa tagatgtttc aagcaatgga
     9061 gaggagattg cttttttcgt tgcttctact acgcgatctt ctccactagc cacaccgatc
     9121 cccatcaatg ctgtaccttg gttttccatc actgttttca cgtcagcaaa gtcaaggtta
     9181 acgtaacctg gcgctgtgat caaatcagag ataccttgta caccttgtcg taaaacatta
     9241 tctgcttcac ggaaagcttc aagcatcggt gtctttttat ctacaacttc taataaacgg
     9301 ttatttgaga taattaataa tgtatccaca ttttctttca aacgagcgat accttctgca
     9361 gcaaaacggc cacgtttcgg tccttcaaaa gtgaatggac gtgtcacgac accaacagtc
     9421 aatgcgccca attctttcgc gatacctgca acgattggtg cagcacctgt accggttcct
     9481 ccacccattc ctgcagtgat gaaaatcata tctgcaccgt ctaaagcttc gcgtaaggat
     9541 tgttcacttt cttctgccgc tttttgtcca acttctggct gagaaccagc acctaaacca
     9601 cgagtatatt tggggcctaa ttggataact gtttcagctt ttgaattttt taatgcttgc
     9661 acgtctgtgt tggctgtaat gaattcaaca cctttaacgt tttcttcaat cattcggttt
     9721 acagcatttc caccgccacc accgactccg atgactttga ttactgcgcc gtcattgata
     9781 ttattatcaa ttgagaattc catatcttat tcctcccagg atgaattaat caaaaatatt
     9841 tgtaaagaaa cccttgattt tattttttac attttctccg gactcttttg gttccggctc
     9901 atcgtagatt gtttcttcag gtgcttcata gctctcatat gtgtttgttg tttcttgttc
     9961 aactaccatt tgacgagctg ctgaggtttc acccgtaaca gcaatttttg ccaattgata
    10021 cacttcactc aagtttgctg aataatccac gatacttatt acatttgtaa ataccgggtt
    10081 tctcagcccc atgtgatttg gaacatacaa ttttacattt acgccgaaga tttcttgtgc
    10141 caagtccaca acaccaggaa gactagcagc tccgcctgtc aatacgacgc cacctggcaa
    10201 ctctaatgct tcgatttggt ccaatgcatc ttttgctttg ttgaaaatct gttccattcg
    10261 agctgaaata atttctgaca agtagcgttc atcgactttc actggttctg actgaccaat
    10321 cacatctact ggaaattctt catctggtga tgtccgttct gggtatgcat ccccgtagtt
    10381 tattttcaac gcttctgcat tgttaaatga cgtatttaat acaatagaga tatctttagt
    10441 gacaaattcg ccaccttctt ggtcaagatt ggtaaacttc aattgcttat catgcataac
    10501 agccgttgtt gtttggccgc cacccatatc aatgacgatc gtaccaaaat ctttttctcc
    10561 gtcagaaaga attgattcag tcagcgctaa aggagtaatc accatttcgt tgacgattaa
    10621 tccggcattt tccacgcatt tacgtatgtt atggatgatc gttttaggtc ctgtaaacaa
    10681 cagaccgtac atttctaatc ttacgccgat cattccacga ggatctttga ttccctcgaa
    10741 cccatcgaca gtaaaatctt gtggcaatat tgacacgatt tgacgttcag gaggtattga
    10801 acgaacaagg gcagctgaag ccacatttct gacatcttca tcggtgattt cctttgaatc
    10861 tccgttaact gcaatcattc cctggcaatt ttctacttca agcatgtttg ctggcaaacc
    10921 gacacttacg cctttgatct ggatacctgc tttttcttct gcttgtcgta ctgctcgttg
    10981 tatcgcttgg accgttttat cgatatcaac gataatgcct cggttaatcc cttctgattt
    11041 tgcgtttcct actccaataa tattcatttg gctgtcgata tattcagcca cgacaacttt
    11101 aacagatgtc gttccaatat caaggcctac atacattcct gtttttgcca tgaatgggat
    11161 tccctcctac ttactattcc atatacaagc gaacaactag cgtccgtttt aaaatatact
    11221 tattaataga tatattcact gcttccaatt ctaccataaa ttactggttt ttagaaagta
    11281 cattcttact ttttaaagtt gttttatcaa ttttcattta aacttgacgg agttgtactt
    11341 ggattatctt ctatttgact tgtttcactt gaatcatttg aatcagatga tccgtttgtg
    11401 ctagacgatc caggtgtttg tgctttttgc gtttctgcct cttcacttga ttcgtttgtt
    11461 tgatccgcat tttcttcact ttcagttgtt tcttccgatt ggcccgattc ttccgtttct
    11521 tgagtgctac cgtaaggatg cgagaagata ccaacttcca tgtcgatcac acctttttca
    11581 tccatatctt tggctacttg cgaataatat ttcatttggt tcgcaagatt attgatattt
    11641 acaataactt gattgccgtc attcatattc aattggatca gatttttatt gcttgctctt
    11701 ggcgtgtact tgatttcaga aattgctttt tgtaattctg atgataactg attgtattgc
    11761 tttgttaatt ctttgatttt tgcagagtct ttgaactctt ccaagatagg gagatttttt
    11821 gtaggatttg ctactttttc atctaatact gttccatttt caatcactgg gtagtattga
    11881 ttgttttttg cgatcaaagc aatctcttta tattctgtta catccaaatc gaattcattc
    11941 atccctttga aatgtatatt tgctgtttcg attcttggct gtgctttttt aaggcgatcg
    12001 atataagtag atcgatgaaa atactgtccc caaacatttt ctcctaatga caattttgta
    12061 tcagagatga tttcttggct gttgactgtc ttattgcctg atactgcaac tgcttgtaat
    12121 ttgctataag gagaaacata atagatcaaa aaaatcagcg gtatacccaa tacagtgatg
    12181 atcaaagcta accgacgaaa tagtactttg tgccgttgac tttttaaatt gggcagccgg
    12241 ttcaaaaaag aaccgttata tggtccaccg tttttctttt gcttgatctc ttcaaaaaat
    12301 tcgatttttt tctcggactg gctttcttct tgctgttcct cttgaaccgt tttttcttct
    12361 tcagaatctg aaaggggtgt atctaatgtt atgtcttctt tttttggctc ttggtttttc
    12421 atctcttgtt ctgccagata ttttcggtta gccttttgcc aaggagtcaa attgttttca
    12481 tcagaagacg gatgagacaa tttttttggt tcttttttct tactcactaa gaccaccgcc
    12541 tttatttttt gattatttgt tttactaagt cataaagacg ttttgaagca tcagggattc
    12601 cttgttcttt agaagctcgg cacatttgct tttgcaattc ttcatcaccc atgatttcgt
    12661 tgactgtctg acttagtgat tcacctgtta gttcattatc tgcaatcatt tttgctgctc
    12721 ctgcgtgaac aagactcatt gcatttttag tctgatgatc attcgtaaca tatgggctgg
    12781 gaaccaacac tgccggtaac cccagagctg tgaattctgc gatcgaagtt gcaccagcac
    12841 gccccaccag cagatcactg gaagccatga cctctgccat atttttgata taaggctgga
    12901 cactaatatt ttgtatttca ggatttattt tatttaggat ctcttcataa tatctatctc
    12961 ctgaagcata caaaacttgg taagaagaag gaatatattc acttaaaaaa actgatactg
    13021 cttgattgat tttaagtgct ccttgactac cgccaaaaat caagactgtt ttcttttctg
    13081 gatccaaatc aaatgttttc aaaatatctg attttttagt atctgctact tcttgtgctc
    13141 gaggattacc tacaagaact gctttgtttt caggaaaaaa ggaagccgca tctttaaatg
    13201 aaagagcgat ccgatccaca tatcggctta aaaatttatt ggtaacacct ggtacactgt
    13261 tttgttcatg gataactgtt ggaataccca ttttggctgc agcgtacact accgctccgg
    13321 aaacatatcc tccagtaccg ataacgatat ctggttgaaa atcactgagg attttttttg
    13381 cttgacggat actcttaaga aacaatcgga ccgtcttgat gttatgcatg gaaatttttc
    13441 gtttaaatcc ttgaatctcc aaggtatgga aaggcattcc tgtatcaggg acgattttgt
    13501 tttctagacc tcgttttgct cccacataca tgaattcagc attcggttct tgagtcttta
    13561 cataattgac aaaggcaaga gctggataaa tatgtccccc tgttcctccg cctgtaacca
    13621 aaattttcat tttttatcgc tcgcttctac tttttctatt ttaatcggtt cacagcttcc
    13681 atgaatctgt ttcctctagt ttcaaaattt gggtattgat cccagctggc attcgctggt
    13741 gataataaaa ctacatcacc atctgtactg ttttctaatg ctaaaggaac tgcttgttct
    13801 acagaatcgg ctgttactat tgtctcgatg cctgcttttt ttccagcatc catcaacttt
    13861 tcttttgttt ggccaaatac gaccattgct ttgattcctt ttaatgaagg aattaattca
    13921 tcaaatgtat ttcctctgtc taatccgcca gccaataaca cgaccttaga cgtttcaaat
    13981 ccacttagag ccattttagt cgctaaaata ttggttgctt ttgaatcatt ataaaatttt
    14041 ctgccttgga tttctcctac atattgcgta cgatgtggta cgccatggaa aaaatgcaac
    14101 gtttctcgta tagcttcatt tgatatacca tacaattttg ctacagaaat agcagctagt
    14161 gcattttcta cattatgctt acctggtacg ccaagttcag atatttccat tattttttct
    14221 tttttatagt atattgagta atcgtcagca tacacaccgt cttccaacac ttcttttgtc
    14281 gagaaaggca gtacgcgtgc ttttgttctt tgcgctaatt cttgtagttc tgactgattc
    14341 cagttaagga tcagataatc tttttctgtc atgttttttt gaaggttcca tttagctttg
    14401 acatattctt ttcttgtacc atgataatca atatgtgctt catagatatt ggtaatcacc
    14461 gctatatgag ggcggaaatc tgtgattccc atgagttgga agctagacaa ttccatcaca
    14521 attttatctt ctgattttgc ttcttgcgct acaccacttg ctggataccc aatattaccc
    14581 gctaaacgtg caattccttg atctgctcct gcattcagta gtagtcctgt catcgttgtt
    14641 gtcgttgttt ttccgttcgt tcctgtaatc ccaataattg ggcattccgc tacttcgtaa
    14701 gctagctcaa cttccgtaat taccggaata cccatttctt gggcttttgc tactaaagga
    14761 tgactgtagg gaatccctgg atttttgacc atcaatgaaa attcttcatc caacaattcg
    14821 attggatgac tacctgttat tacttttatc cctaaagaaa gcagctcttg tgcttccgga
    14881 ttttcgtcaa aaggttttcc atcattaacg gtgacaagtg cgcccaattc atgtaataat
    14941 ttggccgcac ttactccgct ttttgcgagt cctaagacaa gcactttctt attttcatat
    15001 gttgatattt ttttcatttc ccgtcatctc ctaccaaacg atccataatg tgatcaacga
    15061 agtcacaatg ctgatcaacc aaaaaactgt atcaatcttc cactccgacc atccgcacat
    15121 ttcaaagtga tgatgaattg gagacatttt aaaaattcgt tttcctgtaa gcttaaagga
    15181 agtaacttga agcatcacac ttgctgtttc cataacatag atcaaaccaa tcaaaagcaa
    15241 tgtccattct tggtttagca ttatagaaat tgccgctaaa agtccgccca acgctaatga
    15301 accgacatct cccataaaaa tcttggcagg ttttctgttg tagacgaaga aacccagtaa
    15361 acctcctaaa acacttaagc aaatgattaa tacatcatac tgatgctgat gccaagcaat
    15421 tagaccgtaa gctgcaaaag agatacttcc taagccagca actaatccat cgatgccatc
    15481 tgtcagattt actgcatttg aaaatccaac aagccagaag agagcgaaaa taccataaat
    15541 gattcctaaa ggtagatcta gtccgaagaa attcaataca cctggataac cttcagaacg
    15601 gtaaacaaga taaaagatga ttccccctag aatctgtcca atcaatttct gcttcgagtt
    15661 cagtcccatg tttcttttct tgaatatttt tataaaatca tcaagaaagc cgattactcc
    15721 gtataacgct aatacaaaaa gcaagatgaa aagagtagga gttagctgtt tttgccaagc
    15781 tccaacccaa attcctgtca aaatagagcc gatcagaaac actaagccgc ccattgtagg
    15841 agttccggct ttgctattgt gccactttgg tccctcctca cgaatcgcct gaccttgctt
    15901 tttcatttga aaataaccaa taaatagcgg cattgcggca attgtcattg cacaactgct
    15961 gacaatggga atcaatgctt gtgtccattc catgctcttt tctctcctat ttctcttctt
    16021 attcttctaa agtaaaacta agtttgtctt cagtgatttt ttcatatgga gcaagttctt
    16081 gtttgacaca ataaccatct ccatcaaaac taacttcaat accgaggatt ttccccagct
    16141 tcatgatatc tgcttttgac catcctgtta cgtcaggcat cagcttatca ccaccagtat
    16201 ataggatcag tttttctcca gagatcaact gatcaccgtt ggcagtagat tgtttttgta
    16261 cttttttacc attaccgatc actacaggtt gcagtccact tttttgagca tcagctgctg
    16321 caacgtccgc ttcaagatta cgataatcgg ctacggatac tttttctgtt tttgtttctg
    16381 tatctgaatc ttcttcagtt tctttaaaat ccattgcccg tttcatcaat ggatttgcga
    16441 ttttagccaa tgccattcga tcatatgtct tagggtgttt cattgttaga tacaagacat
    16501 agtctggatc ttcagaagga accatctcca caatcgaata caggtaggct gtatctcctg
    16561 tttgatatcc ccctgtatca gaagcgattt gggctgttcc gttttttgcc gatacattgt
    16621 atccaggtac actatataca ccataagcac taccgtagtt ttcgctttcg acaacatctc
    16681 tcatatattc acggactttt tctgttgttt cttttgaaaa aggttgccct agaacttctg
    16741 tttgagttgt tcgctcttct ccagtttggg gatccaccac tttgctgata tatcgaggtt
    16801 gaatcattgt accgttattc gcaatagaag tgaaagcttt catcatttgg aaattcgtca
    16861 cacctaccgc ttgtccataa gcactcattg ctcggtcgac aatatttaat gtaggtaaag
    16921 caccgtttac ttcatcatct aatccagaat gagtactttg tccaaatccc agtttttgta
    16981 aatagttata ccatcgtccg cctaatcgct gttccagtat gaccatcccg acgttacttg
    17041 accatgaaag agcttgacgc atggtcagca cacctttttc accaaagtca tggtcattga
    17101 tcgtagcatc ttctacttgg atttttccag attggaaggt ttcattctcg ttgaattctc
    17161 cttcttcgat agcagctgca gtagtgaaaa ctttcatggt tgatcctggt tcataactat
    17221 cttgtaccaa gaaattacgc cagatagcat cttttccagt caatccttcc attgtttcag
    17281 gattgaaagt aggtcgttgt cccattgcca agatttctcc ggtttttgct ttcatcagga
    17341 ctgctgtcaa ttcttctggc tgatattctt catttacttg atccatcagt gtttctaaat
    17401 aactttgtag tctgctgtct aaagtcgtgt agatatcttg tccgtcgaca gctttttctt
    17461 cttctgccac tgtacctggc agaggatttt gaaaattatc tttttgataa ataatcttac
    17521 catctttacc gctcaagata tcattgtaag cagactccaa acctagttta ccaactaagc
    17581 cattctcatc tttgtctgga actgcgtatc caataaaatg agaagaaaag acgccatttg
    17641 gatacatccg agcctgatgg tcgacaaaat aaagaccagc gacaccttct tttttcatgt
    17701 ccgcttcgat gttttgcttg gtttcttggg agatattttt cccatagcta ccaaattcaa
    17761 cttgatacag ctcttttttt gcgccgtcct ctaaaatatt cagtgcatct tttttgtcta
    17821 tagaaagatt cttatgcagg atttctgcta ttttatcaaa attcttttct tcaacataca
    17881 gttttttatc cccagaggta tatgtttttg agagtatcgc tttaattgaa taagaggagg
    17941 catcttctgc gagcgccaca ccgttacgat cataaatcgt tcctcttttt gcttttacta
    18001 cttcacttcc ttggtacaac tgttttgttt tttcagctaa tgaggtacca gcaacatgac
    18061 ctccaataac gatatacgag aaacggacgg caaataaaaa gaacaatcca atactcgtag
    18121 caaataaaat gatacctact tttttgcgat tgttcatcgg atttagattt ttcttttcca
    18181 taaaccgccg aattttattt ttaagactca tttacttcac tttccttaaa ttgtcgtcat
    18241 taatagacaa tcctttttct tctgcgattt gtttaatccg ttcagtacga gaaagttcgt
    18301 ttttttcttg ttctaaactc gttttttctt gctttttatc attgatttct gtttgtattg
    18361 acgtgatttc tttttcagct ccgctgatat ttgtgcgcag cataactgtt aaaacacata
    18421 aagcaacaat agcaatcgcc acgcttgcca cagcaatttt ttccaaagca gaaatctgct
    18481 ttaatcgttt tgccggtgat tgtggaagaa tgatttcttg attcgaagaa gaatcaggca
    18541 tttgttgttc cggctgatcc accaattcag gtaaatcata ttcgtaatct tgcattttct
    18601 ttaattctgc catcattcta ctcctctttc attcttattt tttctgcaat acgtaacttt
    18661 gcactgcgtg atcgattgtt ttctgttagc tcttcctctg aaggaaggat tggtttacga
    18721 gtaatgactt tcagttctgg ttggaattcg tcaggtatga caggaagtcc cggtggtaga
    18781 tcttgtaccg tactatattc tttaaacata cttttgacaa ttctgtcttc caatgaatgg
    18841 aatgtgatta cgctgattct tccattgact ttcaataacc ggattgcctg ttctaaagag
    18901 gcctcttcgg caccgagttc gtcatttact gcaatccgca cagcttgaaa aattcttttt
    18961 gcaggatgcc cgccttttct tcttgcggga gctgggatga cttgtttaat gatttcgaca
    19021 agctcaccag ttgtttggat tggttttgtt tttcgtactc gttcgatttc acgagcaatt
    19081 tgtttggaaa atttctcctc gccatagcgg aagaaaatct ttactaattc atggtaggaa
    19141 tattcattaa ttacgtgata agcggataaa ggagctgact gatccatgcg catatccaac
    19201 ggtgcatctt gatgataact aaatccacgt tctgcttcat ctagctgagg agaggaaacc
    19261 cctagatcat acaaaatgcc gtctacctta tctacatgct cactcaagcg ttcttctaat
    19321 tctctgaagt tcgctttgat aaatgtgacc ataccttgtt cgacataaga agctaaacgc
    19381 agcttggcgt gatcgattgc tttttgatct tggtcaaacg catacagatg ccctttagaa
    19441 cttagttggc ttaataaata ttcactatgt ccggcaccgc cgagtgtaca gtctacataa
    19501 acgccatccg gtttgatgtt caatccatct accgtttctt tcaacatgac tgtatagtgt
    19561 tgaaatgttt cagtcattag tcagcctctt cctattctta tagtccgaaa tcaatcatac
    19621 tttcagcaat ttcgtcaaag ttctcttctg ctgctgcaga aaaatcttgc catcgtgtct
    19681 catcccaaat ttcaattcgg ttagatacac cgatgactac gcaacctttt gtcaaagctg
    19741 cataatttct taacgtgctg ggtatattga ttcgtccttg tttatcaagt tcgcattcag
    19801 tggcagccga atagaagaat cgcacaaaag tacgtgcgtc ttttttagcc aacggcattt
    19861 cgttcagttt agcttccaga ttttcccatt cgctcatcgg atagccgaaa agacagccat
    19921 ccaatccgcg tgtcagaaca aacttttcgc caagttcttc acgaagtttt gagggcacga
    19981 ttaaacgtcc ttttgcatca atattatgtt ggaattcacc catcaacatt gaaagccccc
    20041 cattcctcca ctaactaaaa ttagtctacc acaatccccc actttctacc accttttctg
    20101 ggaaatccgg gaacaacttt ttttattcat aaacattttt ttaaaaatca agctgcaaaa
    20161 agccttttaa aagacttttt acagcttttc tatttttttc ataggtaaga aagtatgttc
    20221 acaatgatca agacagcgta caagacgatt gtcaacagaa aaaccaaacg ccaagtcatc
    20281 ttgaaataac gcggataaat aatttcttca taataatatg cttggaaaac agcaatacaa
    20341 attcccatta gcaaaacaga aataaagaaa aacggtaaga tagaaattgt ccctgtattt
    20401 ctagacaatg catgcatacc gccccacaaa aaaggaatcg ccaaatcagg ggcttttatt
    20461 ttaaaacgac gatttaacga aaataatttt acaagaaatt ggcaaccaaa caaaacgatt
    20521 accgggtaaa tataccaaaa caagataagt ggtgtgaatg tgcccatgaa cgtaaactcc
    20581 ttttttatcg ttgcttcaag tatactgtat atactttaca caactgtttc attttattta
    20641 aatttatgca gttatattct atatcttaca aacacattcc atgtattcca attccctatc
    20701 cgttataata aaagaaacag gcaacaggag gaaaatcatg atcaaatatt ttacattaga
    20761 taatgagaaa ctgcgtaata ccggaaagga aactgaccaa acgatctggt attgtgtaga
    20821 acgtcctaat caagaagaga ttcatcagct gacacatcaa tttgaaattc cgtcagatta
    20881 catcacttcc atattggacg atgcagaaaa ttcccgtaca gaagaattca atcaatcaaa
    20941 gttgacaaag ccagcgttat tattgttaca atatcctttt gctaaaacaa gtcctagcgg
    21001 ttattttcaa gtggatacat acccgctctc tattattttg acgcccaaaa agaagcttat
    21061 cacaattacg aatcatgaac cactgttttt aaagaaagtc tttagccaga cctttccaaa
    21121 aagtgatatt tcgcctaatc tcaatatctt ctttcaattc atttggcagg tcacactctc
    21181 ttacaacagt tatttagcta cgcttcaatc tcaatgcgat actttggaag ccaaacttca
    21241 agtatccact gaaaacaaac aactttatca aatcatggat atccaaaaaa gcctcgttta
    21301 tttcagagaa gcgacttctg ctaatttaga tgcattgaaa gaactagcgc aaaataaact
    21361 tttccaagaa aatcatgctt tgaaaaatca tttacgtgat gtattggtgg aaacagagca
    21421 ggcaatgact acttctcgca ttcaattaaa actagtagaa caaatgaatc agactttttc
    21481 agcaatcgta tcgaataatc tgaataatat tatgaaaatt ttaacttctt taacgattat
    21541 tttaactata ccgacaatcg tcggcagtat ctatggaatg aacattaagt tacctattgc
    21601 tgaaagggat gatgccttta tatggctagt tctattaaca ttatttattt gtatcgtaac
    21661 tacctattat ttaaaaaaag ggaagttttt ataaaaaagc cctcttgtgg aaatggaaaa
    21721 aagattgtac acttgaatca actaattaat gacgaggttg aaaacaatgc gtgaaaaaaa
    21781 acaaacaagt acttttgcta aagtaacaaa atttgtcatt tggacaatgc tgattttaac
    21841 gattggatct gttgtattga ctgctgtaat gagtgtcatg taaaaataaa atatagctat
    21901 cagctgctga aaaggacctg aaccactgcg aggtggctca ggtccttttt gcgagtttca
    21961 atagttcttt tgcatgttcg agtgtgagtt ttgtaatctc acttccagcc agcatacgtg
    22021 caatttcttc ttctctttct tttgttttca actctgaaac agaagtttgc gtacgtccat
    22081 caatcactgc tttcacaata taatattgat agtcggcgac tgcagcaact tgaggcagat
    22141 gagtgatgca caaaacttga gagtatttcg atatttgaga aattttatct gcgatggcct
    22201 gtgcaactct tccgctcaca cctgtatcca cttcatcaaa aataatactt gtcacacctt
    22261 gctcagaaga aaaaatagtt ttcaaagcaa gcaacatccg agacagttcg ccaccagaag
    22321 cgactttcac caaaggcttc agtggctctc ctggattggt cgtaatgtaa aattcgattt
    22381 ggtcaaatcc atcatgattc aattgtctat tttcttcttt taagaaacgt acctcaaact
    22441 cagtgttttc catatacaga cttttcaatt catgcagaat ggatttttcc aattcttttg
    22501 cgattttttt acgcgcttca tgaagatctg cggcttgttg ctgaagtaga agctcttttt
    22561 gctctagttc ctctttcatt ttgtccaatt gaccttctga aaaatcagaa gaagctagct
    22621 cttcggtgat ttcgtcatag taagccaata ttgactcaat ggattcgccg tatttacgtt
    22681 tcaactgtcg gattaattct aaacgttgcg taacttcttc taaacgactc tcatctaact
    22741 ccaataaatc aatctgacga ctcatatcgc cgattgcatc ttgcagcaaa tagtaagcac
    22801 tttggatatt ttcataaatt gcttcatact ccacatctag atgggcgatc ccttgcagtt
    22861 ctgaaacggc caagccaaca ccatctacac tgttttgttc attattcgtc agtgctccgt
    22921 aacctgcagc taatccatca gcaatttttt gataattgct aagtttttct ctttcttctt
    22981 ttaatttctc ttcttctccg acttgcaaat cagcagcagc tatttcttct tgctggaaat
    23041 ggagcatatc tattctctga acataggatt tttcattttg ctgcactttt cgtacccttt
    23101 tttccagctc acgataagag atatattctt gctgataact ctttttcttc tgttggaaag
    23161 ctacatctcc aaaacgatcc aataaagcaa gatgtgattc aggctggagc agttcctgat
    23221 gttcgttttg tccttggata tctactaaat aactgccgat tcttctcaga ttagctaatg
    23281 tgataatatg accattcact cgacaaacat tttttccagt taaggacata tcccgtcgaa
    23341 caatcagatt gtcctcatct gtttcgatcc ctaattcaac catcagttca gagaatcctt
    23401 cttgttttgg tagttcaaac aacccttcaa gcacacattt ttctgctcct tgacgtatat
    23461 aatcgcttga acctcttcct cctgctaaca gacctaaagc atcgataata atggatttac
    23521 ccgcaccagt ctctccggtc agcgcggtca ttccttcgtg aaaagaaagc cgcaattccg
    23581 gaataatcgc aaagttagta atactgattt cttgtagcat cacattcgct cctcaactta
    23641 aaggtaatgc aggaagtctt tcttcaataa aactgcctgg tcttcagttc gtgtaatcat
    23701 caaaacatta tcgtcatcat tgataatcga aaataattct ttcttgtaac gtttatcaat
    23761 caaatttgct gctgccgaag catttcccgg caatgttttc aaaataacaa atttttccat
    23821 ttggtcaact gccacgaaag cgtcttttaa tagtttttcc aatttagtat ttacatcttc
    23881 gtttgtttca gctggtatgc tataacggta gccgccatct gaagccggta ccttgatcag
    23941 tttcagttcc tttatatctc tggaaatcgt agcttgagta acagatattc ctttgctttt
    24001 caggatttca acaaattctt cctgctttcg gatatcttgc tcactcaaca atcgagtaat
    24061 caaacgatgc cgatcttttt ttctcattac gtcttcgctc cttagcatat tcttcttaat
    24121 caatgatagc ttatttcctt ggtataataa agaagaatcc gcatacttct cacttgattt
    24181 tcacagttac aatttattta tggtcaaact gttcgtgtgc atttgaaacc acttctgcca
    24241 tattaatagc tgaattgatt gttccgcttt cgggtacttt cctcaagtga gccaagaact
    24301 cgatatttcc ttctcccccc gtgatcggag aataacttaa ttccagcaca tcatatccac
    24361 cttgtgccgc aaatgacaaa atgtcttcta aaactttttg atgggtctga gggtcacgaa
    24421 caattccgtt tttccctaca gcttctctgc ctgcttcgaa ttggggtttg attaaagcaa
    24481 tcacttctcc ctcttctttt aaaatagcat gtaatggagg cagcatcagt tttagtgaaa
    24541 taaaagaaac atcgataaca gcaacttctg gaattcctaa ggtaaagtct tcgggttttg
    24601 cataacgaaa attcactctt tccattacga cgactctagg atcttgtctt attttccaag
    24661 ctagctgatt gtagccaacg tctaaagcat aactcatttt tgcgccattt tgcaaagctg
    24721 catcagtaaa tccgccagtg gatgcaccaa tatctaacaa tgtttttcct tcaacagata
    24781 gatcaaatgc tttgagtgct ttttctaatt ttaatccccc tcgagaaaca taaggaagtt
    24841 ttttaccttt gactcttaat tcactactca ctggaatttt ttctcccggt ttatcaaacc
    24901 gctcgttttt ttcgttgtaa atcaaaccag ccatcactga acgtttggct tgttctcttg
    24961 tttcaaataa tccttgtttg accgctaata catctacgcg ttctttttcc attctttccc
    25021 tcatttctcc acatcaaatt gcttgatcat ctggcgcatc agcccgccat caaaagaaaa
    25081 aacgtcctct tctaatttgt ctatgatctt gttggcggat aacagctcaa tctccaacgc
    25141 ctcacgtgtc ttttctaggc ctaataaacg cgggtaagta tttttttcca tacgttcatc
    25201 tttaccagct gttttcccta atgttttcgt tgtaccaatc acatcgagta gatcatctct
    25261 tatctgaaaa gctaaaccta aatgttcggc taatcgttga agtagcaata agatttcttc
    25321 aggttgcttc gctaatatgc caccagccag caaggcataa cgaatcaaat gccctgtctt
    25381 tctttcatga ataaatgcta attcttctaa agaaagcttt ttcgattcgc cctcgatatc
    25441 tgcagcttgc ccagcaacca taccttgtga tcccgcacaa acagctaatt gctgaagcag
    25501 cagaagctta ggagaattac ctaagtgtgc catactgatt agctggaatg ctcctgtaag
    25561 caaaccatct ccggccaata tagctaatgc ttcgccgaat actttatgat tggtaggttt
    25621 tcctctacga agatcatcat tgtccatcgc aggcaaatca tcgtggatca aggaatacgt
    25681 atgcaccatt tctaaagcag ctgctacttg atagacggga acatcaatag gctcatcaaa
    25741 ggaagcaacg gccgttaaaa gtaataaggg acgtattctt ttcccgcctg ctttcactga
    25801 ataactcatc gcatcataga gtcctctatc cgttgtatgc tcatctaaaa aagacaagat
    25861 ttctttttct acagcaggta gatgaatagc tgaaaagtca gttaatctca tctcagcttt
    25921 cctcgtttcc gtcaaaatca acttcttcat ttgattctgt catcattttc gtcaaggtct
    25981 cttctgcttt agctaatgta tcttggcaat gtttgcttaa ttccattcct cgtttaaacg
    26041 aatctagagc tgattctaat ggaacgtctc cttgttctaa ctgcatgaca attttttcta
    26101 attcttgcag tgactcttca aaagttggat tttccatatt tttattctcc tttgtctatc
    26161 tcttgtactt gtcctttaac gattccatct tgataatgga tcgtcagcaa atccccttga
    26221 tgaatctcgg tgacactttt tactacttga tcatttaacg tcgtatagct gtagcctctt
    26281 cccatgattt tcaatggact caacaaatca agagattgaa cagcttgagc aaaacgctgc
    26341 tgctgttgtt tcatatattg ttggattttc tcttctaagc gtttttctag ataagcagtt
    26401 tcttggcgag ctgctttaac tcgataaagc ggagccactt gttccaattg gtggcttaat
    26461 gcgatcgttt gtttttcttt cgcatgataa atttgctgtg tagcttgata taatcgctgt
    26521 ttcaactggt ctaatttgat ggtctgacct tcatacaatc tttcaggctg ccgaaataca
    26581 taggaacgtc gagcacgctc gaaccgttct tgtttgcgct ggatctggcg tacaaacgcc
    26641 tgctgcaatc tagcttgccg ttcttgaatg cgcatcaact cttctgacaa aacaggaaca
    26701 gctaattctg cagctgctgt tggagtggca gcccgaacat ctgctactaa atcagcgatc
    26761 gtggtatctg tttcatgtcc tacagatgaa atcaccggcg tattagcttg ataaatcgcc
    26821 cgagcaaccc gctcttcatt aaacggccat aaatcctcaa tcgaaccacc cccacgaccg
    26881 atgatcattg tatcgaaatt gcccatctct tctactctct ggatattcct gacaacattg
    26941 tccgctgctt ggtttccttg tacaactgtt gggaacaaaa ccaattggac aattggatat
    27001 cttcttttca ccgtcgtgat gatatcgcgg ataaccgctc cgcttggact ggtaaggacc
    27061 gcgattcgct ttgggaaacg tggcagcatt tttttaggcc cttgaaaaag gccttccttc
    27121 atcagctttt cttttcgttc ttccaattct tgatataaag cacctattcc atcaggctcc
    27181 atgtgctcta catagatttg ataagaacct cccgcttcat ataatgaaat acgcccaacg
    27241 acaaggactt tcattccttc ttttggctgg aatcgtaatt tttggaatgc tcctttgaac
    27301 ataatagcag atatttttgc atggtcatct tttaagctga aatattgatg ggcattggca
    27361 cgtaaacgga aattcgatat ttcgcctgtt aaatatacgc gttctaaata aggatcggca
    27421 tcaaattttc ttttcaaata cttcgttaat gtagtgacag ttaaatattc ttgtgccatc
    27481 tattcacctg ctccaaaagc ttcggcagat tttacggttt gatacatcag catcgtgata
    27541 gtcattggcc ctacaccctt tggcacagga gtgatccagc ctgcaatagg ctccacttca
    27601 tcaaatttca catcaccgat cagcttaccg ttagcatcac gattcatccc tacatctatc
    27661 actacagcac ctggttttac gaattctttt gtgacaaaat gtccacgtcc tattgctacg
    27721 actaaaatat ctgcttcttg tgccagttca gccagatttt cggttttaga atgagcaaca
    27781 gtaacagttg catctgccat caagagtagc tgagccatcg gtttaccgac gatattgctt
    27841 cgtccgataa ctactgctcg tttacccgcc ggatcgatag aataagcttc aaacattttc
    27901 atgatgccgt atggagtaca aggaatcttg tccggtttac ccgcaaggag tctgcctaaa
    27961 ttcatcggat gaaaaccgtc tacgtctttt tcagggcgga tagctaacag gatcttttct
    28021 tcatctatat gctttggcaa tggtaattgg accaatatac catgaaaact aggatcttga
    28081 ttgtattttt caatttctgc aagtagttcg tcttcagaaa tcgtctcagg ataacgctct
    28141 accaatgaat ggattccgat ttcttttgct acacgttctt tatttcttac atagatttgg
    28201 ctggctggat tttcccctac caataaaacg actaatccgg gatggatacc attatctttc
    28261 aatttttgga ttttttcagc aatctctgcc tgcatttttt cagctaattc ttttccgtta
    28321 atcaattctg ccatctaacc actcccagat attttttgtg ttttcattct accatagaat
    28381 tttcccgatt tataacaggt ttatgatgat tcatagaaaa aagctaggac ttacgcccca
    28441 gcttctaatt ctttcaatac attggataaa acaccgttga cgaatttacg tgaacggtca
    28501 tcactaaatg tttttgccaa ctcgattgct tcatttaaag caacagccgc tggtacatct
    28561 gtcacataag tcatttcaaa aatcgcgata cgcaagatca ccaaatccat ttttgagatc
    28621 cgattgatgt gccaattgtt ttttaaatgt ttttcaatca ctttatccaa ttcatctttt
    28681 tttgcacaga caccgccaac tagcgtatca agataaacag gaacaaactc tgattcatct
    28741 tcgttgatca tgtcgcggtg gtctaattcg attgcgttga aaatcgcatc ttcttttgtt
    28801 aggtctgcat tgaagtctaa tggaaataaa gcctgcaatg ccatttcccg aatctcgtgt
    28861 cttgataatt ctttattcat tctcttcctc ttcgtcttca aaaagttctt cgattgttgg
    28921 ttgttctaat ttttcaggaa caacagctac tacatggata ttgacttcag ataaatcgat
    28981 atccgtcatg aaaaggactt gctggctgac ccgttcctgc atttccattg ctactttagg
    29041 aactgaaaca ccatattcta gataacagta aagatctact ttcaagccat cttcctcgtt
    29101 tgctaagtaa acaccttttc cgtgagctgc acgtcctaat aattcagtga cattgctggc
    29161 aaaagttccg cgcattccgt atacgccttc tactttagaa gctgcaatcc cgataattac
    29221 ttcgatgact tcaggagcga tcacgatctc tcctaaatct tggtttgcgt ttaaaatcaa
    29281 atttttttca tctgccattt atttattcct ccctttgcct aagcacgaga tacatccgaa
    29341 ttctctatga tgcggcatag tgatcacgtg gtaagaaaac tgccgccgat cagctgcaaa
    29401 ctgctatgaa ttggagaaaa cacccaactt tattatctct tacagcttaa aagttcttct
    29461 atagtgtatc attaaatcga gtgattttct ataacaaagt ctgtgaacac tgatttttac
    29521 gcttacttct tctattgatg aaactataag atgatcaatt cttttggtga acgagtcaac
    29581 acacgatttc catcttctgt gatcaacaag tcatcctcaa tacgtactcc accgattcct
    29641 ggtaagtaga tccctggttc atcagtgatc acatttcctg gtacaaattg cttttctgca
    29701 cgagaagaaa cgtttggtcc ttcgtggatc tctaaaccga tcccatgtcc tgtagaatgt
    29761 ccaaatgctt ccccgtaacc atgttttgaa atataatctc tagcaactgc atctaattgg
    29821 acaccagtca ttcctggttt tgcagcatca ataactgcta attgagcatc taagactatt
    29881 tgataaattt cttttaattt ttccccagga tcaccaatag cgaatgtacg tgtcatatcg
    29941 gaaacgtacc cttcgtaata gcaaccaaag tccaatgtga tcaagtcacc ttgttcgatg
    30001 atttttttgc ttgctacacc atgcggcata gccgaacgca atccgctagc aacgatcgta
    30061 tcaaaagaaa cacctgaagc gccaagagaa cgcatataaa agtctagttg atttgcgact
    30121 tcaatctcag tcatgcccgg ttggatcatt ttcaggatgt gatcatatgc catatccgca
    30181 atatgacatg ctttttcgat aatagcgatt tcttcttcat ctttcacttc tctgagttct
    30241 tcgatcatct gtgaaacagg tactaattcc gcatcgatga tttcttctaa aaccgaatat
    30301 tctaaaaatg agactgttgt ttcctcaaaa gctaatgagt gcagttcttc tttgcgtact
    30361 aagtttgcta cctcatcaaa aataggtccg acatttttaa taatttcaaa cccttgagcc
    30421 tgcgctgccg cctgctcggt ataacgaaaa tcagtaataa aaaatgcttt ttccaaagta
    30481 ataacagcta atcctgtcgt acctgtaaag ttcgttaagt aacgaagatt gtacggactg
    30541 gtaaccaaaa atgaatccaa attttcttct tgcatttttt tgcgcaattt ttctacacgt
    30601 agcatcataa tgaaactccc tctttctttg cacgcttttt tccattatag caaacttttc
    30661 ttcgtacacg acagttaaat aaaaatttcc ggaaaacttc tttttatttt aaataaataa
    30721 agccaaaaag gagtcacagt ctcgctgcaa ctccttttag tttactattt tgtaaaaact
    30781 tatgatcgtt ttccaaagaa aaatgagaca actgctacca aaatcactgc gcctaaaata
    30841 gatggaataa gtgccatacc tgcagctttt ggtccccatt cgccgaaaat cgcttgtcca
    30901 atacttgaac cgactagtcc agcaatgata ttcgttacac agcccatcga atttccttta
    30961 ctggtgatcg cacctgcaat tgctccgatc acggctccaa caatcaatgt ccataaccaa
    31021 ctcataatat aacctccttc tttgctaatc aattttagca tatgggattt aaaggggaca
    31081 aataattggt tttgctattt catattttct tctgcaaagt aaaaaaataa agaaaacctc
    31141 ctcttagaag ttttctttat tttttacttg cgtttaaaca tactacgaat accacgtttc
    31201 actacatcaa agaaactcaa tgattgtacc attctatctt catctacata ttctctgatc
    31261 gcacgaagca cttttttagg ttctttggaa gaaaatgtgt atgtaccatt ttttttcgtt
    31321 tgaatggcat atcgaggaat ccattttccc tttagcatga ctgatgctag tacataatcc
    31381 acttcttccc atggaatttg gatatatttg cgtctatctc gcgcatggta aaactcaaaa
    31441 ccattattac ctatcatgat ttgcccgtaa tctgtcagac ccgtaaacga agtagcatca
    31501 attaccaagt ctacttttgt attgattgat tgaaccattt tctccctcca gatatttgtt
    31561 tatgaacagt atactatata aaaaaagctc gggacaacgc ccgagctata tttttatgga
    31621 ttataaaagt ccgatgacgt gtccaacaac accgactacg aataaaccaa ggatgataac
    31681 gattggagaa acctttttct taagcaacca catacataac aatgttaatc ctaatgcagc
    31741 taaaccagga atcaattggt ctaagttgtc ttgtaatgtt gttacctttt caggagaaag
    31801 ggctaagcca ttattaactt gttcaaatgc actcttgatt ccttcaccac ctgaaggcaa
    31861 tttatcccat tcgatgtaag cacctttatc taattttaca gttgatacaa ctggtaagaa
    31921 tttgattgat acccatcttt gtacaagggc agctaatacg aacatcccta aaattgaagc
    31981 accttttgtt acgtcttgta acaatccgcc agaaaggtca tcagtaattt tagaaccagc
    32041 tttgtagcca aattcttgtg tataccacat gaatgcccaa cgaataatgt tccaacctaa
    32101 gaagaagatg attggtccaa ggatatttcc tcccatagct aatgaagcac ctagtgcacc
    32161 tagcattgga cgtactgtaa accagaacac tgggtcacca acaccagcta aaggacccat
    32221 catacctact ttaacccctt gaatcgctac atcatcaaca ggcgcgccat tcgcacgttc
    32281 ttcttccaga gccaatgtaa cccctaaaat tggagaagca atgtatgggt gtgtattaaa
    32341 gaattccaag tgacgtttta atgctgcaga acgttcttct tttgttttgt ataatttttt
    32401 gattgctgga atcatagaaa atgcccagcc accgttttgc atacgttcgt agttccatga
    32461 accttgaatg aaggtagagc gccacgcaac agctaaacga tctttttttg ataatttgat
    32521 tttttcttct gccattttat tttcctcctt caagattaat agtcattcaa aatgtcgcct
    32581 aaagggtcac cagaattgct tccgcctcca ttatttgatg aaccacccat tttagaaagg
    32641 ttcaagtaaa taagagctaa tgcaacacct aaagcaccta gagcaatcaa tgttaattga
    32701 gaaattgcag ctacaacgaa accaataacg aagaatggcc atacttcttt tgtagccatc
    32761 atgttgatta ccaaagcgta acctacagct acgaccattc ccccaccgat agccatacca
    32821 tctgttaacc atgctggcat tgattcaagg aatgattgca cagtttgtgc tgggataaat
    32881 agcaacgctg ctgctgggac cgcgatacga accccttgta aacatacagc aatgatgtgc
    32941 cacatttcaa ccttacggat gtttccttct tcagcagccg catccatcaa atgaacgatt
    33001 ggaacagcga ttgtacgtac gatcattgtt aagaacaacc ctgcaacagc caaaggaacg
    33061 gcaatcgcga tcgcagaagg aacaccttta acaccttgtc cacctaaaac taaaataatt
    33121 gctgatgcta ctgatgctaa tgcagcatct ggtgctacag cggcaccgat atttgcccaa
    33181 cctaaagcga tcatctgaag cgtaccgcca agtacgatac cagcttcgag gttaccggta
    33241 actaaaccga tcaatgtaca tgccactagt ggttgatgaa attgaaattc atcaagaata
    33301 ccttccatac cagctagaaa ggcaactaat acgactaaaa ttattgaaat aatagacatg
    33361 ataagcctcc tataaatttt aatattattt attacgcttt tgctaattca gcttttgctt
    33421 ttttcaaaat atcatccatg ttatcgcgtg aatcattagg tactttacgt acatcgaatt
    33481 tcacaccttt ttgttcaagc ttctcaaacg cttcaacgtc ttcttgtccc atagacaata
    33541 ctttacttac aacgacttta ccgaccgaat gcgccattga cccaacattt aattctttga
    33601 tgtctacgcc accttcaaca gctgtcaata cgtcttcagg attttcaaat aaaagcaatg
    33661 ctttggtatt tccaaaacga gggtcttttg ctacttcgat cattttgcta ataggaataa
    33721 catttgcttt tacacctgga ggagcagctt gttcgattag tctcttacgc agatcatcct
    33781 ttgatactgc atctgaaaca acgataatac ggtttggttg cactgctttt gtccaagctg
    33841 tcgcaacttg tccatgtaac agacgtgagt caacacgtgc taatacatat ttgatttttc
    33901 cgtctccgac cacagttcct tctggaagtg ctcctttagg ttgtgcgtct tcaactgctg
    33961 ctttcggtgc ttctgccggt tctaattctt ccggttttac ttttacaccg tcttttgctg
    34021 tttcaatgat gtgtgctgca atttcatgag cagattccat tgaaaagcgt gatgcgtagg
    34081 cttcaatcaa cattggtaaa ttcaaaccgc tgacgattgc ccatttatct ttgtgctctt
    34141 cgaataatgt gtttgattga ttgaacggtg ttccgcccca taaatcaact aagaataaca
    34201 cttcgtcttg attatcaaaa gaagcaaccg cttcttccaa tttggctctc aagtcatctg
    34261 gaccttcact tggtttcaaa atcacggctt tgacgttttc ttgttcgcca aagatcattg
    34321 aaccggattg taagattcct tcagcaaatt gtccatgact tgctaggata atccctacca
    34381 tatttatacc tcctatttac ttttgttgta acaaattcaa aaatattaat aattgctaac
    34441 cattacaaca gaattatttg accttttcca acaaccattc ttgctacacc tttttgattt
    34501 tcgctagtag ttccatcaaa aggattttcc tgtcagcagg tacttttcga atctctaact
    34561 ccacttcttg actgttcaag taacgaaagg aatcaatatc ttgttgattt actgaaacaa
    34621 aattggtaat cattgatttg ccttcagtaa aactcatacc gccgatattc aacgacttca
    34681 actgtacacc gcagcggaca accgcttcaa catcttgggg attggtgaat aaaagcatca
    34741 cttttgctcg tgataataat ccactatgat agatttgaat cattttctgc acggttatta
    34801 catttgctgt gatccccggc ggtgcagctt cttttagtag aaactttctc aatttatctt
    34861 gagcgactac atcgctgatc acaatgatac gttcaacacc tgtttgcttc gaccaaactg
    34921 ttgcgacttg cccgtggatt aaacgatcat ctatacgtgc caatcgaata tccatctgca
    34981 aaactccttc ttcctcacta cttcacagaa acccctatca tttctgtgtg aacaaccacc
    35041 tcctagcaca cactactata agcaaaaagc gtgccaactt ttttacgata cactaattga
    35101 atttaaagga ttcttaacca atacacaaaa acgatacact ataaaagtgt atcgtttttg
    35161 tgtattcatg gcacagtgta ttctttttta tctaactgat tatggactag acgtagcaga
    35221 taatacattt cttctatcgg cacttttact tttatctgat cttccaacag aacaactgag
    35281 gctgccaatt ggctataaag aggatcattc gttgtatttg tcaattcgtt ttcttccaca
    35341 gtcaatggtt cattccgaat aatacgttcg atcattcctg ctgtgtgcat cactaggttg
    35401 attttgaatc catattcttc atcgccgatt cccgtcgtct gagagatttg tgtagaaaat
    35461 tgccacattg gatcgattaa cttactacca ttaataaaga tgaagttatc agagataaac
    35521 tcaacacacg tatttttagc tgactcttca tctagggaga tgaattcagt ttcctccaat
    35581 tctgattcca tcagcaactg atccaaaatg acttcgatat tttgatcaat aaaacgttct
    35641 aaaggaataa atggggcttg gatattcaaa tcagtaatcc cagtagtagc tatgatctga
    35701 tattcttttt ggataactgg cagttcttct ttcacttcca caatcgacaa agctagaact
    35761 tctaattctg tttcttgacg cttgtttacc gctcgttcga tcaattcttt gattctttgt
    35821 gctgtacctt cgccagatgc gcagatagcc aaaacagctt ttttcttatg aataggtttt
    35881 gcttcctgaa cagattctac agaagatatt tctgcgtaac ctcgaaaatt tttcaatgat
    35941 tcatgcaaca tatctagatc tgtccctaat actgagacct ttcgtacagt ttccaaaact
    36001 agagaagttg ttaccatatc aacagtacgt actggaattc cagtgtctct ttgaatttgc
    36061 gaattaaacg acgcaaggga ccccatatct actaagagta tgaccccgct tccttcatct
    36121 acttcttgta cattgcgttc aattcggtcc aatgctgttt tcggatccat atccaaaggc
    36181 atgtctactg ctcgtacgtt ctctgcatct aacaactgtt ggacaacttg gaccatactg
    36241 cttgctgtgc tgtttccgtg agctgcaacg actacaccga ttctgccgct tgcttgattt
    36301 gcccgtaacg agacgagtag aacggtcaag taatagtctt cactttctgg aatctccact
    36361 tgaaaatacg tttcgatata actacgcact tctttcgcaa cagcatattc atctggaaaa
    36421 tcaaaagcca tctcacggat gttatcattc gtatgccgtt cttctcctaa gttgattttt
    36481 ttcaaaaaag aactgatatg caagctcata gcataaataa agttttgctg aaactcatat
    36541 ggtaaagatc ttttgaccat tacataaatc tgattggtca cttctatgat tttcggatct
    36601 acaaattctg ctaatttgct gtctgcatta aaagaaaaac catggtcttt atagaaagac
    36661 ttcagatgga tgttgatatc tgtagaaatg aaatgattga ttgcttcttg atccaaacca
    36721 tccgacttca gtaaagctgc tttatctccg atgatatcat atagattata aggtaactca
    36781 taggagtccg attggatttt catgatcgta tcatttggtg ctacagttat ctttggctcc
    36841 aatattttag aaagctctgc cattgcactg cggttgcttg ctaattgaat caatccgcta
    36901 cgaatccctt ctgtcaaatc atcgattgtg atcgagattt cgggtgattg catatgattt
    36961 agaaatcctc gtgcacatac taactggata tttgacttca attgtcctat atttccatag
    37021 gtcacactgc cgatcaatgc ttttgctaca tcttctgtca aagaaatctt tcgctgcgtg
    37081 cggtttgctt cgtgggcaat catgattttt actaaatcga ctttctcatt tgcaggacgt
    37141 ttttcaaaag ccggaagttt gatattgatt gggatacgcc gaacaaaagt ttctaataga
    37201 gaagaacctg ggtcttctgt ggtcgcacct acgatccgta catttgcttc atgggatttc
    37261 gttgtttccc caagacgact gtaagttcca tgatccataa agtaaaagat catttcttga
    37321 ccttcaggcg gcaaccggtg aatctcatcc aaaaacagca taccgccatc cgcttgatcg
    37381 ataatcccca ttttttcttc atctgcgcct gtaaaagcac cttttgcgta cccaaataaa
    37441 tgactcatca aaagctcagg gttattggca taatcagcac aattgaaaac aatcagttcc
    37501 gtttcctcat ctattacatg atgcgctttt gcaaaatgat acatcgcgtg tgcaaaataa
    37561 gtctttcctg atccggtagg tcctgtaatc aaacaattta atcctcttgg cggatacaaa
    37621 atcgcagctt ttgcctgttc cactgaattt ttcatgctgc cgttagctcc aatgattttt
    37681 gcaaagatat ccttagtatc tatttggagt gtattttttt cttcagagac cggcgtttct
    37741 ttgtaactga ctacatgttt tgtaactggc tttacgtgcg taacgagtgt ttgatatatg
    37801 taacgtactg gtcgtccgtc tgtctttatc agttttcctt cgcgcactag ctgattcaga
    37861 tctttactgc tatttgttcg ctgaatccct aacacttcag caatttcctg tgttgttact
    37921 ccttgatcat attctgcctt tgttaaatga gcggtttttt ctttgacata catataaatt
    37981 cggtcaatgc gttttgccaa tggtatcgct ccttttctat agactattgt acttaggctt
    38041 caaaacataa tactatgtat ttatttctgt ttcgtcaact atttacctga ttttgttttg
    38101 ttgataacac gcggatagat agaaatgttc aatttaaaag aacgaaaaat cccgatacga
    38161 tttttataat ggaaacaaga aaaagaacac tgtttaccat ataattattt cactgatagc
    38221 aaacaatata gcttccatca attgaaaacg atagttatca aaacatcctg attccaaatt
    38281 ggcaaaaaaa tattaagtgt aaaaagacaa agtaaacttc gtagcctcca ctacaacttt
    38341 tatttttata aacaaaaaag gctgtgacaa aagtcttgtc acaacctctt taatcataag
    38401 attcaattaa gcttcttgag ctactggata aacagacact tgttttttgt tacggccttt
    38461 acgttcgaaa cgtactacgc catcaacttt tgcaaataaa gtgtcgtctc caccgatacc
    38521 cacgtttaca cctggataga tttttgtacc gcgttgacgg taaagaattg atccacctgt
    38581 aactgtttgt ccatcagcac gtttagcacc tagacgtttt gattctgagt cacggccgtt
    38641 agatgtagaa ccgccacctt ttttgtgggc aaataattgt agattcatag ataacaacat
    38701 ggtctgcacc tcctatttat cattaattgt tttaatttgg atatattctg agttttcagc
    38761 ttctatcgat tgtaatccta ataaaagatt ttctaataga atctcagcaa tattgttttg
    38821 ttcttgtgtc attcctgaag tgacttcggt ataaagataa ccgccttctt cttcattcat
    38881 ttcaacaatc ggctcaaaac cggctaatga ggcaatcccg ttgacagtgc tgatcgctaa
    38941 cgcagaaaca cccgcacaaa cgatatcact tccataagga ccagcatcag catgcccggt
    39001 caaagtgaat gaaacgatct gacctgcgtc atttcgctta aatgttcctt tgatcatata
    39061 taggccttct ttctcaaacg ctattaagcg ttgatcgcgt cgataaccac ttttgtatat
    39121 ggttgacgat gaccttgttt acggtgtgaa tgttttttag gtttgtattt gaaagtaacg
    39181 actttctttt gtttgccgtg tttttcaaca gtaccttcaa cagttgcacc tgcaacagtt
    39241 ggagcgccta cttttgtaga ttctccgcct actaagataa cttcgtcaaa aacaactttt
    39301 tctcctgctt caacgtcaag tttttcaaca tagattgctt gtcccacttc aacttttact
    39361 tgtttaccac cagttttaat aattgcgtac atgctataaa gcacctcctt ctgatatact
    39421 tagactcgcc atccgaagtg acagcatatg ctgtgcttat tacttgttct gagcggttgt
    39481 agctgtggag tccacaatta caacagtact agcttaccag aacagcaacc gttagtcaac
    39541 taaatttaaa atcaaaaagt aaaacaaaaa ttcaagcaat acttctgctt ttctatcctc
    39601 acagttctac caaataaaat agtcgcattg tgtttctttt attcttataa taaaagaaaa
    39661 aggaggagca taaatggaat acccaaaaga actgatacaa gaagcttctt taagaatgaa
    39721 cggccgtcct gtagaaggat ttatcgctgg gcaaggacct cttcatccta aactgatgct
    39781 cgtgggagaa gccccaggga aaacagagat tgatacgcat gttcctttta gcggtcaagc
    39841 tggaaaagaa ttgatgcagg cactttcttc aactggctta acacgagaag aggtgtatat
    39901 caccagtgct gtaaggagcc gcccgtatcg cgtgactcat cggatcaata aacggacaca
    39961 acagtcagaa actgtttatc caaatcggac acctactcgt tccgaagtat ttgcgcatgc
    40021 accaatattg gattacgaac tgatgaaggt ccagccaaaa ttaattgcga cacttggaaa
    40081 cattggtctt caacgtttac ttggcaaaga atatacggtt accaaaaatc atggtcaact
    40141 ctatacaggg cctgtcttac aattaacaga acaaaaggat gcctacgaat ggtcagaaaa
    40201 aaactatcga attcttccac tttttcatcc agcagctatt ttctacaatc gctcccttgt
    40261 acctgttatc catcaagact ggcaagtact agcttcactt ttacagcaat attaaaaaga
    40321 acagacaaga ccataaaatc ttcagtttac agtcttgtct gttctttttc attgttttaa
    40381 tggctaattg cagtatattt cgcatgtaaa gctgtcaacg catgagcaac tgtttgatgt
    40441 tgatgcgtga atgtatgttc ccacaagtga gaaagttgtt tttctacttc tggctgcaag
    40501 gaagtataga taatcgtttg tcctctttgc tctgcttttt cttgaaattc atcaaaaact
    40561 gcacgtgccg tcaaatcaat acttggtacg cctcgcatgg agaaaatgac accatcgtga
    40621 tgatttagtt cttccaattt atttttgagt ttttctgaag acataaaaaa cagtggacca
    40681 ctaatataca ctactgtcca gttattcagc ttttccgttt ctggcagccc cattctttgc
    40741 caatcgatcg tttctaatga aatcgtgata gcagcgcttt tgacaacaaa aaatacacag
    40801 ccgccgatca ccccgatcac gatagcaata cttaaatcaa agataactgt acaaatcatc
    40861 gtaaggataa acaatagaac tgctgaccaa tatttattag taaatagctc cttgatcgtt
    40921 tcccattcat tcatacgcca agccgtcaca atcaaaacac ctgcaagtgc tggcattgga
    40981 atctgtgaca tcactggcgc aaaaacaagc atggagagca gtaagacaac tgcatgtatc
    41041 ataccagcta atctagtttg tgcaccagat ttgatggcca cgcttgtacg ggcgatggcc
    41101 gcagtagcag gaatcccgcc aaagaaagga agcaacagat ttccaattcc ttgcgccatt
    41161 aattcttgat tgctatctaa gggcttattt gccattcggc cagccgaagc accacatagc
    41221 aagctttcaa tcattcctaa taaagcaatg ctaatagctg gaactgccac ttgttgcaag
    41281 gcagaaaagc taaaatctcc catattcaaa cgattactac tgatcaatgt ttgtggaatc
    41341 ttacccacag ttgctactgg taaatggaac aatagcataa ctgctgttgc caaaatgata
    41401 gccagcagcg aactaggcat tcgtttcgcc catttttttg gatagataaa catccctaaa
    41461 atgactagcc ctcccatgat caaagaggct gtgtttatcg gaaatcctat cgtttgataa
    41521 ctacttagtt tttcaataac gtttgtacct tccgagtgaa caccaaataa attatcgatc
    41581 tgacctaatg caataatgat agcaatacct gaagtaaacc ccgtaattac gggtgctggg
    41641 ataaaagaag tcagtgttcc taatcggaaa agtcctgctg caagtaaaaa tagacccgct
    41701 aaaaaagtag caagcaatac accttgcatt ccctttgttg ccgcaataga catcaagata
    41761 gctgccatcg cacctgtcgg acctgatatt tgataaaaac cgcctgataa accgccaata
    41821 acgataccgg caatgatagc cgtaatcaaa cctgcggccg catcagctcc gcttgatacc
    41881 ccaaaagcca aggctaaagg caatgctaca gcagctactg tgatcccagc caaaagatct
    41941 ttctgaaatc ttgaaagatc ataacctgaa aattcttttt ttagtgagga aacataattt
    42001 ttgaacacct atctaactcc tttttcatga aattttctaa aataccattc attagaatat
    42061 catatttcat gagataaagt aatatttttc acaaagtttc ctgttagata aattcttatt
    42121 ttttctatat ttataagaaa atagaaagca atgccaacag agctagccca ccttgtttga
    42181 gtaaaatcca acgatcgcta gtcaaactgc catataatgc aacaaaaaaa atagatatta
    42241 atagcatact tgtgatttct ttagatgcgg aagaaagata agctccataa agtagtccca
    42301 ctcctaatag tccattgtac actccttggt tcttaaacaa tgtttgaaca gattttctgc
    42361 ttaactcttc tttactcatt ttaaaaaccc tacttgtcgt gtcagaatca gttctcaccg
    42421 tttcgatata catgatataa aaaaattcaa ttgcaactag agtcactaaa attctagata
    42481 gtatcgtcat atcttcttcc tcttttctga tgagttttat ttttttgctc cttataattg
    42541 tactcgataa ggatgttttt ttattttatt tgcctttgaa aacaaacaat attggcacaa
    42601 aaaacaaaga aaaaccgtta gaaacctcag tttctaacgg ttttttagcg tcacaggagg
    42661 gattcgaacc cccgaccgtc cgcttagaag gcggatgctc tatccagctg agctactatg
    42721 acaagaatca gcggaacatc aataattata gataattaaa ttgcttctgt caatacgaaa
    42781 tagttattta attagcaaat aatgaattat tttggcccaa ctactaaatc aatcttttaa
    42841 aacgagacat tctccttcag ctgttctaag gttatcaatt gaaaacagga gctacattga
    42901 cttcatgcta ctcctgttta tttagatttc ctgtatcctt taattgactt acaaatatag
    42961 aaacttttac atcaagttcg aattgagccc tcgattcaat cgattatcta caggctgcca
    43021 taatcatgta tcacaactcg attgtttctg attcctttgt cattaagatc acaccatcac
    43081 ctaatggcaa gattgtcgat gtcaaatcgg gatgatccat cacaacacga agaaattcat
    43141 ttaatttacg atggatagct cgtttgccac ggggaatctc ttcatctggg agcaagatcg
    43201 ttcctccttg gaagatgtca tctaccatca aaacgccacc ttttcttaac aaacgtaagc
    43261 attcaggtaa aaattcaata tattttgatt ttgcactatc cataaaaata aagtcatacg
    43321 gaccagacaa ctccggcaag atatctgctg cttgaccttc aagcaaagtc actttatctg
    43381 tcagtcctag acgttcatag ttttttttag ctttctcgat catgacatcg aaacgatcaa
    43441 ttgtcgtaac atgagcgttt tctccgattg tcgtggccat gacacttgat gaaaaaccga
    43501 tagctgctcc gatctccaag attcgttcag gctgaagctg tccaagtaaa aatttcaaaa
    43561 atacaactgt ttcatgggga ataactggta cttctgctac atgagcttct tcttccagtt
    43621 tgcctagctc acccggtaat tttttttgct gggtacgcat aaaatccaat agttcttctt
    43681 tgactacagg acgatgcatc atttcattgc gcatactact cctcctcttc acacattata
    43741 cgaacaactg actaaatcta caagctgcat gagtgttttc tcgtgtttca tgttcagaag
    43801 agtcgttgga tctctggaat acatttatgt ttttaacatt cttacaaaaa tttctgtgtt
    43861 cgtctccgct tctttttcaa ctctacaaag gtattccatg ctgtgattcc tgttaggcaa
    43921 aaagcaatcg taaagaataa cccctgaatc catgcaaatg ccgtttttga aaggactagt
    43981 ccaatatatg gaatctgaaa gatgggacta ccgataatat ttctcttttt tactactcca
    44041 ggctcaatat aagtctttgt atcagctttt gtataaaaat cttcgtggtt gttttcaacg
    44101 gctattacgc gacgcgtcga gatttttttt cctgaatttt cataatatgt aataatatct
    44161 ccgactaaaa tctcatttgt atttttcggt cgtacatata tcagatttcc ttgtgcataa
    44221 tatggactca tttctttgtc ctcaataata taaggagtca aaccaaaaat tcggggaacc
    44281 aagacgacga gaagaataag aattaaaaat ataatcaacg ttatagaact tataaaagtt
    44341 atttttttgt aatcaggtat cgttttcaac ttctttcttt attcaatcct tcttaatcat
    44401 ataataacga taaatttttt caaattatag ctgtaaaaat gtttcccttt aataattata
    44461 tgtttctaat tctatttcct catctttatc tcatttttta ttataaataa ttgacgttag
    44521 tttaaaaaac gtttacccta acattacaag ttttagaaag gaata
//
LOCUS       DAYYKN010000009        44280 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000009 DAYYKN010000000
VERSION     DAYYKN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.009"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(118..1461)
                     /gene="vanS-B"
                     /locus_tag="ACHBB7_000399"
     CDS             complement(118..1461)
                     /gene="vanS-B"
                     /locus_tag="ACHBB7_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM9208381.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            complement(1464..2123)
                     /gene="vanR-B"
                     /locus_tag="ACHBB7_000400"
     CDS             complement(1464..2123)
                     /gene="vanR-B"
                     /locus_tag="ACHBB7_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM9208382.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            complement(2741..2908)
                     /locus_tag="ACHBB7_000401"
     CDS             complement(2741..2908)
                     /locus_tag="ACHBB7_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208383.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            complement(3066..3251)
                     /locus_tag="ACHBB7_000402"
     CDS             complement(3066..3251)
                     /locus_tag="ACHBB7_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM9208384.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            3396..3767
                     /locus_tag="ACHBB7_000403"
     CDS             3396..3767
                     /locus_tag="ACHBB7_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9208385.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            4025..4354
                     /gene="mobC"
                     /locus_tag="ACHBB7_000404"
     CDS             4025..4354
                     /gene="mobC"
                     /locus_tag="ACHBB7_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM9208386.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            4315..5643
                     /locus_tag="ACHBB7_000405"
     CDS             4315..5643
                     /locus_tag="ACHBB7_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9208387.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            5640..6011
                     /locus_tag="ACHBB7_000406"
     CDS             5640..6011
                     /locus_tag="ACHBB7_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM9208388.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            6194..6430
                     /locus_tag="ACHBB7_000407"
     CDS             6194..6430
                     /locus_tag="ACHBB7_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9208389.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(6730..6933)
                     /locus_tag="ACHBB7_000408"
     CDS             complement(6730..6933)
                     /locus_tag="ACHBB7_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM9208390.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMLVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(6937..7881)
                     /locus_tag="ACHBB7_000409"
     CDS             complement(6937..7881)
                     /locus_tag="ACHBB7_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208391.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            complement(7882..11802)
                     /locus_tag="ACHBB7_000410"
     CDS             complement(7882..11802)
                     /locus_tag="ACHBB7_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM9208392.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            complement(11951..14032)
                     /locus_tag="ACHBB7_000411"
     CDS             complement(11951..14032)
                     /locus_tag="ACHBB7_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM9208393.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            complement(14029..15258)
                     /locus_tag="ACHBB7_000412"
     CDS             complement(14029..15258)
                     /locus_tag="ACHBB7_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM9208394.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            complement(15248..15499)
                     /locus_tag="ACHBB7_000413"
     CDS             complement(15248..15499)
                     /locus_tag="ACHBB7_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM9208395.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            complement(15522..17513)
                     /locus_tag="ACHBB7_000414"
     CDS             complement(15522..17513)
                     /locus_tag="ACHBB7_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM9208396.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            complement(17545..19944)
                     /locus_tag="ACHBB7_000415"
     CDS             complement(17545..19944)
                     /locus_tag="ACHBB7_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM9208397.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            complement(19874..20296)
                     /locus_tag="ACHBB7_000416"
     CDS             complement(19874..20296)
                     /locus_tag="ACHBB7_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM9208398.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            complement(20310..20846)
                     /locus_tag="ACHBB7_000417"
     CDS             complement(20310..20846)
                     /locus_tag="ACHBB7_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM9208399.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            complement(20883..21746)
                     /locus_tag="ACHBB7_000418"
     CDS             complement(20883..21746)
                     /locus_tag="ACHBB7_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM9208400.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            complement(21838..22053)
                     /locus_tag="ACHBB7_000419"
     CDS             complement(21838..22053)
                     /locus_tag="ACHBB7_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM9208401.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            complement(22130..23938)
                     /locus_tag="ACHBB7_000420"
     CDS             complement(22130..23938)
                     /locus_tag="ACHBB7_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM9208402.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            complement(23935..24411)
                     /locus_tag="ACHBB7_000421"
     CDS             complement(23935..24411)
                     /locus_tag="ACHBB7_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM9208403.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            complement(24486..24698)
                     /locus_tag="ACHBB7_000422"
     CDS             complement(24486..24698)
                     /locus_tag="ACHBB7_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM9208404.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            complement(24720..25685)
                     /locus_tag="ACHBB7_000423"
     CDS             complement(24720..25685)
                     /locus_tag="ACHBB7_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM9208405.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            complement(26176..26697)
                     /locus_tag="ACHBB7_000424"
                     /pseudo
     CDS             complement(26176..26697)
                     /locus_tag="ACHBB7_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(26713..27411)
                     /locus_tag="ACHBB7_000425"
     CDS             complement(26713..27411)
                     /locus_tag="ACHBB7_000425"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM9208406.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(27368..29029)
                     /locus_tag="ACHBB7_000426"
     CDS             complement(27368..29029)
                     /locus_tag="ACHBB7_000426"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM9208407.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            29247..30647
                     /locus_tag="ACHBB7_000427"
     CDS             29247..30647
                     /locus_tag="ACHBB7_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM9208408.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(30899..31978)
                     /locus_tag="ACHBB7_000428"
     CDS             complement(30899..31978)
                     /locus_tag="ACHBB7_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9208409.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(32064..33023)
                     /locus_tag="ACHBB7_000429"
     CDS             complement(32064..33023)
                     /locus_tag="ACHBB7_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM9208410.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(33035..34546)
                     /locus_tag="ACHBB7_000430"
     CDS             complement(33035..34546)
                     /locus_tag="ACHBB7_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM9208411.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(34568..35131)
                     /locus_tag="ACHBB7_000431"
     CDS             complement(34568..35131)
                     /locus_tag="ACHBB7_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9208412.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(35137..36000)
                     /locus_tag="ACHBB7_000432"
     CDS             complement(35137..36000)
                     /locus_tag="ACHBB7_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9208413.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(36014..36910)
                     /locus_tag="ACHBB7_000433"
     CDS             complement(36014..36910)
                     /locus_tag="ACHBB7_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9208414.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(37085..38374)
                     /locus_tag="ACHBB7_000434"
     CDS             complement(37085..38374)
                     /locus_tag="ACHBB7_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9208415.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(38346..39863)
                     /locus_tag="ACHBB7_000435"
     CDS             complement(38346..39863)
                     /locus_tag="ACHBB7_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM9208416.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            40061..40951
                     /locus_tag="ACHBB7_000436"
     CDS             40061..40951
                     /locus_tag="ACHBB7_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM9208417.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(41024..41848)
                     /gene="nadE"
                     /locus_tag="ACHBB7_000437"
     CDS             complement(41024..41848)
                     /gene="nadE"
                     /locus_tag="ACHBB7_000437"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM9208418.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(41860..43332)
                     /locus_tag="ACHBB7_000438"
     CDS             complement(41860..43332)
                     /locus_tag="ACHBB7_000438"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM9208419.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(43969..>44280)
                     /locus_tag="ACHBB7_000439"
     CDS             complement(43969..>44280)
                     /locus_tag="ACHBB7_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM9208420.1"
                     /translation="YPKVVEFFFGNTAINYYKLFSLDRTQLLLVDTYDKKQVVMINTK
                     KKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFKRPNKDIVVIEQKNTKDS
                     I"
BASE COUNT        10847 a        10978 c        10033 g        12422 t
ORIGIN      
        1 ttattatggc aagcgcatct gtactttccc tgtagccagt ctgtagtttt cctgtagcag
       61 caaaaaaaga ccggcagggt tatacctgtc ggtcaaaatc atttaagttt taaatattta
      121 caatgttgat gtgagcggta aatccagcca gaacaaaacg ccatccgagg tgttttccag
      181 cgcatactgg aggctcatgg catccagcgt tttttgtacg atggcaagtc ctaaaccgct
      241 tcgcccactt tttctgcttt gcgcctgatc aatgcgatag aatggggtga acagccttgg
      301 aagcgcagta tcatcaatgt gaacgcccat gttcaaaaca aaaaggcggc atttttcagc
      361 accaggctca ctccatatcc gtacctcgcc tccctgcggc gtgttctgaa ctgcattcaa
      421 tatgacattg gatagtgcct tttggagcag cctcggatcg gacaggacaa tttgcccggc
      481 tggaatatct gtgacgaacc gctggttgtt tgcctctgcc aaggtttgaa aatcgggcag
      541 caactcggca accgtgcgcc ctatgtccaa cggttcagct atgggtacga ttctcccatc
      601 attcaggctg acaagctcca gtatttcgga aatgattttg ccctgcctat ccatcatttt
      661 gatgcattcg cgcagatact tagaatgatc tttgtagtca ccgatatttt caagcattcc
      721 ctccaacaga acgcttgtag ccgcgatggg cgtttttagc tcatgagaag ctgccgcaaa
      781 gaaatatcgc tgtgtttcct ccaactcatg ttcccttgcg atttcatcct ccagccttgc
      841 gatggtttct ttcagcctga catacatgga atgcatatca tgagccagtg cgccaagctc
      901 atcctttcgc tccagcggcg gcggtacatc tttcaggttt gccattttat tcgcactgtc
      961 cgctaaggct ttgatcggcg ttgtcatttg ccgcgcaaag atatacgcgc ataaaaggct
     1021 gaatacaacc attatcgcaa tcataacaat tccccgaatt gtcagccctt gataaagcaa
     1081 tcccacacct gccttgcttt gagcaacaat cgaaatatta tcatctctat gtaccacata
     1141 aagaaagtcg ggtctaaaac tatttgatgt attggcattt ggtgtggcat agagtacgct
     1201 tccctcttta tcttcaatat aaaactcaaa ggattggtta ttgtagtgaa acagccctgc
     1261 cacctcttga atatcaagcc tatcgctatt ctgaatcagt tccaccaatg gctgatagga
     1321 ttttactgtt tgctgtaatt ccatcactct gaaataagac acaaattgct gtgcaaacag
     1381 tgttgctgtt acaccgacaa gcagtagcag ggcaatgatc gtataggaaa aaaccttaat
     1441 gaaaatccct tttctttcca ttattatgat tcctccaatc ggtatcctac accgcgaatg
     1501 gttttgatga tattttccgg cagcttggcc cgcaaatttt tgatatgagt gtggactgtg
     1561 ctgccatcac cctcaaagtc atatccccat atacgtgaca aaatgatttc atgcgttaag
     1621 gttctgcctt tgttctgcac cagcagcaaa aggatttcaa attcttttcg tgtcaggggc
     1681 agctctgtac cgtcacaaag taccgtgaaa tcctccggca gaagtgtcag cctgccgaca
     1741 cggatttcct ttgccagcgc accgctgcgc cgcagcaggg cttccacccg ctttagtaaa
     1801 atctgcatct tgaatggctt tgttacatag tcgtctgcct ctgcatcaaa cgcccggatt
     1861 tggttttcgt catccgacag ggctgtcatc atcagaatgg gagtatcatt tttcgcacga
     1921 aattcacgca aaagttcatg cccgttcata ccgggcagca taatatcaag aataaccagt
     1981 tgataagtgt tttcgtaaaa cttggtgtat gcctcatttc catctgtgca ggcatccacc
     2041 tgatatcctg cctcagccag aaacgccctt actgtattac agatatgatc atcatcctcg
     2101 acaagtagaa ttcgtatcga cataaaaatc cctccatttt aagaagataa cataacagtc
     2161 tgtagtttcc ctgtagtttc aaagcgattt cttttcggat tttaatcatc cgtcattggg
     2221 atactcgctt cttttcatgg tgtatgtatc tatattattg gtttaaacga attaaatcaa
     2281 gataataata attaacacct tcttaatgtt tgggatgttt cgattacagc ggttgtcaaa
     2341 acatcccgat aatactggca agcaatggat cagaatatcc tttacggata ttctgatcca
     2401 ccacttgccc gcctgcggcg ttctcgtttt gcttgttggg gacaccccaa gcctcagcaa
     2461 aatgcgcggc ctcttttttg cgaatgcacc ttgcggtatg gcttttcaca caggcagggc
     2521 ctgtgtgtac tccctccggt cgctcttgcc gcactccgcg cagcagattc agtcctaata
     2581 ccgttcaaga cagtagaaaa tcagcaagtc tatattcctc atatcttcat catccgtgtc
     2641 cttgcagtta caggaaatgt aataatcgcc aaatgtaaat cccagaatat tttcctcaaa
     2701 aagccgtacc tccccggtat taaaatcaat caccccatac tcaaaggcgc attagaccag
     2761 cagttatcca atgtctggag acttcaccat aaagaatcca gatgccggcc ttttcaattc
     2821 taccatatac ggctgataca gcagcagctt cctcgtcttt gtttcgggca ttttccattt
     2881 ctgtcatgat aaagtttcgc ttcttcatac atccatgtgt aaatcttttc tttctgtttc
     2941 agatgagaat actttttatc ggtctggagc agcctgccat ctatcatctt atagtttttc
     3001 cgtgacaaaa taccatgctt tctctctctt caaaaccata tccaaaatgc gccggtttca
     3061 ttatactatt cagtaaccgc ctttcctttc tttgtacgcc tgtcaatcgg cttttccgca
     3121 gttttgggaa tgagccagat cgtcgccatc tttacagcgc cggggatgcg tcccgcagca
     3181 caatagtagt ttatccatcg tgggggcaca ccccatttct cgcctgcctc tttcaacgtc
     3241 atgtagtcca tagtacacct ccacagggta cattataatt cttttgctcg aagaataaaa
     3301 gaaacaaaat gtgaaatata aactttaaga aaggtgcata taccaacatt ccacgggcag
     3361 agtatgaaat atacaatgta agtgggaggt gcgatatggc gaatattgaa gattgtcccg
     3421 gctttgaaac tttcggcgcg gatgtgaaag cggcccggca agcaaagcgc gtttcgcgca
     3481 aagcaatggc agagaaaata aatattgact ggcgctatct tgccaacatc gaaaacgacg
     3541 gggccattcc cagtctgccg gttgtcatac agcttattaa agcctgcggg cttcctgtgg
     3601 aacgctactt taacccggag atcatgcggg aggaaagcga actgcggcaa agggtcagcc
     3661 acaagctgaa actctgcccc gaagaatacc tgccgatcat cgagggcgcg attgacagcg
     3721 ccatactaat tgaaaagccg aagaagaatg aaacggagga tgtgtaactg gcatcctctg
     3781 ttttactttt agcgggagct gcacagcgta gcttctattt ttttgcactt ttttctttcc
     3841 agagggctat tttgcgggta gtttggggct ttggaaacgt cttgttagtg gagaaacagt
     3901 aacgtagcaa gcatagggag tgtagccact cccggaaaaa cggcctgccc cgtccctgcc
     3961 gggccgcgtc cgttttgctt gttggggaaa tccccaaacc cctgaatgga aaggacggtg
     4021 aagcatggca aacagaaagc gaaaaatcgt gctgcgcgtc cctgtgacgc cggaggaacg
     4081 ggctatgatt gaacagaaaa tggcgctgct gcatacgaag aacttttcag cctacgcccg
     4141 gaagatgctc attgacggct atatcgtcaa catggacacc agcgacatcc gggcgcagac
     4201 cgccgagata cagaagatcg gcgtcaacgt caatcagatt gcaaagaggc tgaacggtat
     4261 ggggccggtg tacgcacagg acatcgagga tataaagggg gcgctggcgc agatatggca
     4321 gttacaaaga tacatcctat caagtcaacg ctgaaaaaag cccttgacta catcgagaac
     4381 ccggacaaaa ctgacggcaa gctgctggtg gcctcctttg cctgctctta tgaaacagcg
     4441 gacattgaat ttgaaatgct gctggcgcag gcataccaaa agggcaataa tctgggccac
     4501 cacctgatac aatcctttgc ccccggcgaa gccacgccgg aacaggccca tgagatcggg
     4561 aagcagcttg ccgaggaagt gctgcaaggg aaatatccct atgtgctgac tacccatatt
     4621 gataagggcc acgtccacaa tcacattatt ttctgtgccg tggatatggt aaatcagcgc
     4681 aagtacattt ccaacaaaca gagctatgcc tatatccggc gcaccagcga caggctgtgc
     4741 aaggaaaacg gattgtccgt ggtgaagccc ggccagagta agggcaagag ctatgccgag
     4801 tgggacgccc agcggaaagg gacgagctgg aaagcgaagc tcaaagcagc cattgacgcc
     4861 gctatcccgc aggcaaaaga ttttgatgat ttcctgcggc tcatgcaggc gcaaggctat
     4921 gagatcaagc cgggaaagtt tatttctttc cgcgctcccg gacaggaacg ctttacccgc
     4981 tgcaagacat tgggggaagc ctacaccgag gaagccatca aagagcgtat caaaggccgc
     5041 gttatcacca gagcgcccaa agagcgcaag gggatttctt tgcggattga tctggaaaac
     5101 aacattaagg cccagcagtc ggcaggctat gagaggtggg cgaagctcca caatttgaag
     5161 caggccgcaa agactttgaa tttcctgact gaacatggga tagacaccta cccggatttg
     5221 gaaagcaaag tggccgagat caccgccgcc agcgacgagg ccgccgctac cctcaaagct
     5281 atggaacacc ggcttgcgga tatggcggtg ctgataaaga atatttctac ctacaagcag
     5341 ctctggcccg tggctatgga ataccgcaat gcagcggata aggcaaaatt ccggcgtgag
     5401 catgaaagca cgttgatttt gtatgaagct gcggcaaagg ccctaaaggg acagggtgtg
     5461 aaaaagctcc ccgatctgta cgccctgaaa gccgagtaca agcgattggc cgaggaaaag
     5521 gaacggctgt atgagaagta cggcgaagcg aaaaagcaga tgcaggaata cggcatcatc
     5581 aagcagaatg tggacggcat tttgcgtatg acgccgggaa aggaatggac gcaagagcta
     5641 tgagtattcc acgaatgaat taccctcaat atcgcaaggc ccggaagctg acgcatgagt
     5701 gctgcaacta ttgtgacggc aactgcctgt tgctggacga cggcgaagaa tgtatttgcg
     5761 tacagagcat ttcctattcg cttctgtgcc gctggtttaa ggccgctgtc ctcccgctgg
     5821 atgcggccct gtatgccgag atcaccaaag accgggacga agtgaaacgc tgctgtgagt
     5881 gcggggccac gttcacgccg aagtctaacc gggccaaata ctgcgcggcc tgctccaaac
     5941 ggatgcgccg ccgcaaagag gccgagcgcc agcggaaaag atactggaaa cctacgcact
     6001 tagagctgta aaagccctta tatttcaagg cttttccggc atggtcagcg gggtatgtgg
     6061 gtgttttatc ctatccccgc cgaagtgctg ttctaaatgc gtagaattta ccagcagaaa
     6121 agcgccgttt tctctggttc catgcttccg aatatggtat catagaaaaa atcagtctga
     6181 cgggggtatc aggatggacg gacaagaaaa gctgctggac tatgaaacga tcaaagccgc
     6241 cgttgccggt gagaggtggg ccacggaaaa agtgttggcc cattatgctg actacatcaa
     6301 cgagctttcc acggtggaga tcagacaggc aaacggcaaa aagaagaaag tcattgacga
     6361 agatatacgg cagcgcattt ccctgaaact gctggaagca ttgccgagct tcccgctgga
     6421 acagagatag acggcaagag ggctgcaccc tcttacgccg ctttgcggct atcacctgca
     6481 cacccctgaa acagatacac ccgctgcgcg gctatatctg tttcaggggt ctaattatag
     6541 ctgcaaccta cgcatttaag ggattgaaaa ccgcccgctg caaggcttct cccgcatgtt
     6601 tgcaggggca agcgaatact tcatcctatc cccgacaata ccgggttcta aatgcgtagg
     6661 aataggcaaa aagaaaagac gccggacgca agcgaccaca tggccgccgc gcccggcgtc
     6721 tgctgttcgt tattcgtctg tgaagtggaa cttctggccg tcatagtcgg catccgtcat
     6781 gcgttccagc ttggaaaggg tctgctgggc ctgcgcccgc atatccccat ccataaccag
     6841 catgacggcg gtgagcctgt ttatcgttct gcgccggtct gcattgtggt acatacagat
     6901 caggttttct tcctccacgg taaagtgcat cattccttat acctccaatt cattcttttt
     6961 ggttctggcc gccgcctttt ttgggctggc ggcctgtttg ctgtcggata gctgcttgcg
     7021 gatagagggc cggggcctgc gaatactcac atcccggcgc tcgtcccggt taatcacggc
     7081 atacacgcca tgcctgcctt tcatcccggt aaaacacagg gatttatagg gcagcatggc
     7141 aaagagccgg tcattatcct tggtggaagc aatcgccgca aagtgcgggg agatctccac
     7201 catgaaatgc gttttattgg ggctgttagg cgcggacaga tctttcaggc tgtccacaat
     7261 acgccgggcc tcggtttcaa tccggctgtc atgcaaagcg gtgacgtgga ctttgaaatc
     7321 gccggggacg gcccgccccc ggctgtgctg ctccgtccgt agcgcctcct gcagcacagc
     7381 cgggtcatgg ggctgggcct catagtacaa aaagtttccc aatacccggt cagggcttcc
     7441 gtcaaaacgc tggctctccg ggatgtcacg ggtgccatgc tcataaaaga gccggttcac
     7501 actcaccggc tgggcggttt caatgacgtg ccggaaatgc tgcggatagt ccagctcata
     7561 gagattgccc cggatagtgc cgccctgtgt gccggtcagc tccaccgcgt aggccagtat
     7621 cttgtcacgg gtctgctccc cgtaaaattt ccatgtgttg tactgtcggg tgtctttcag
     7681 gaaaacatca cgttcccgga aacagtacgt cccggagggc cgggacatcc agagcagggt
     7741 cttgtctgcg gcctgatcgc tggcggcatc ccgcctgata atgtctttgt caatgccaaa
     7801 gtcactttga taaaacgcgg tgttctgccg catgatctgt tccagcgcgg ccagaacgtc
     7861 cacattttca aacttctgca tttactctac ctccaatccc tttgattttt cccgcgccgg
     7921 tggctgcttg gcctgttcgg tcttggccgc gttgagctgc gcccggatag agggcttttc
     7981 tttctccctg cgggccgcaa gctgtaaggc gctgggcttt tcgccggaaa ggggctggat
     8041 gcacttgata cggtcagcga cgcggattgt ggtgtcgttt aactgccgct gccatgcccc
     8101 ggccttgggc gaccagcgga aaccgttgct tttcagctcg tcgcgtacag cagcatccgg
     8161 cttcccctca aagaagattt gcaggcggtt ggccacccgg ttgatctcca cagtgccgcc
     8221 gtcaaactcc catcccacat aggccgcctc cttttggcgg gtcaattcct caatgcggcc
     8281 ctttaaccgg cgtatctcgg cgttattgtt ggagagctgc caggactgga acggtttttt
     8341 ctcatagtgc cagcccgacg ccatatccgc tttcaggttt tcaatgcctc tttcggtcag
     8401 atgggggcat ccgtccagcg tcccatgctt gcggtagtag gcattgacgg ccttcatcaa
     8461 ctcctgcgac gcttccagct tttccagttt cttttccagc ttggaaacgg cctgcggatc
     8521 gtcggcgctg ataccaccca tgccggtgct gcggattttg tcaagcaagc cctgaatgta
     8581 ctgaaattcc tccatgttgc ggtcactggc ggcgttctgc ttttctttcg cccgtaccgg
     8641 gaaattggac ggcccggcaa tcaggacaga ggggacgcgg gcggtaatct catagccctt
     8701 attcatgttc tcggccagct tgcgggcgta ggtgtcaagg aggctgtcta tcttttcgtg
     8761 atactctggc tccacacgct ttttctggcg ctccgcaatt tccacggcct gatcgacata
     8821 gtgccggtat tccgccgtgg cgctgccggg ccggtagtca gaaaagctca tggcttcctt
     8881 ggcgcggcgg gcggctccct cattgatggg ataatagcgg acagccgttt gcgggggcgc
     8941 agcctccggg ggttcctcct gtgtctggcc ggaagcaact gccacatcct caaaagatag
     9001 ctgttcgggt tcctccggct gcggttcggg cggcgtattg ttgataatgc cgtcaatcat
     9061 gttttcgttc tgctccgtag acagttcggc ggtacgcagg ggattttccc gcaaaaactc
     9121 cggcacttcc atatagccga agctgtctac atagtgggcg gtgtcctgtc cattctggtg
     9181 cagcaccacc acgtcggaaa cggaaaggct gtgtccccta aaatctgccg ggtggtcgat
     9241 gttgaatgtg cggtaaatat cctccaaaga cgtgctttta tccagcggtg ccgtataaac
     9301 aaggtcatag ttggcactgt ccacggccag ccctgcggcc tgtaaacgct cgtatggctc
     9361 aaaccgatag tcgcgggttt cctccccgcc tcgaagctgg tagatggaaa aggtgtcctg
     9421 ttggggtgct tcatagccag ccgccgcgta ttcctccacg gtcataccgg ctgcggcggc
     9481 ctcctgcgcg atctcggcct gtacctgtgc tttgtagtcc tgcaagctct ggtcgaagtc
     9541 agccagtttc ggcggctccg gcaactgata ctggccctga ttgagcatgg ggcggcactc
     9601 ctgcacataa gagagaagcg cccccaaata ggccgtttcc tccggcgtca gggtttcccc
     9661 ggactgtaag cggccctgcg ccgtctgttc catttgggca aggctgcaat gcagcttcat
     9721 ataggggaca aactcgccta acagcatatc ccgatgggcc ttgtcctgct cccagccctc
     9781 tatgccggtt gtagcgatta catgcttttt ccagttttcg tcctgcgcgt aaaattggtg
     9841 gtagctctgg atatgttcga tcagggaacc gtccctatct ccaaaatcct gccgcccctc
     9901 gtagctgtcc ggctcgccct gaaagataca gtcaatgcgg aacgcggttt tgtcatagcc
     9961 tgcctctgcc ggatatgctg catccagacg ggcaaacagc gcgtcggcct catgcaacgg
    10021 catttgctgg ccgtccttta gctgggggct ttcgctccat agcacagtca caagcggctc
    10081 ggctgccgga tcaagggccg gggcctgttc ttcctcggcc tgcgccgggg ccgctgcctc
    10141 cggggtaacg tcaaccgtga cggcctcctg ttcctgccgg gcctcgcgct cctgctgaag
    10201 ctgggcaaaa tggccgtcga tctcggttat caattcgctg gccgtggccc ggatggtttc
    10261 aagggaggct ttcagctcgg ataactccct gccgctgctc caatcggcca catagccaaa
    10321 agaatagtcc gacgtgtcca gcccgtaata ctggcagacg gtataggcca cgctttcggc
    10381 ctgtacctcg cgggtgctgc ggtctggccg ggccgcctgt tcctcggcgg gggcctcccg
    10441 gtcaatggcg tggagcttgg catgggcaat ctcatggatg gcggtcttta gggtctgcaa
    10501 ttcgctcatg ccctcgttga tggcaatgcg ctgttttgcg tagaagcagc ggcccttggc
    10561 cccgtctgaa atcggctcaa tgcttatggg atagggtgat gtccgttcca gcgcggcaaa
    10621 gaaatcctga tactgctgca cgtcgccggt aagttcactg atatagggca tgggaaaatc
    10681 cttgccctcg gtctggctga tgtcaaagac tgtcaccacc ttaaaggccg gaaccgtcac
    10741 ttcctgttct tcggtcacgg gcttgccgtc cgcgccgatc acgggctgct gggtggccgg
    10801 gtcgattttc tccatctgct gcttaacctt gaacggggcc ggggccagta tcttaatgcc
    10861 tttctcgccc cgcttcacat ggcggtcaaa ttcatcctgc cacctgttat agcccgccac
    10921 aatcgaaccg ccctgcatgg cgatcagcag cgtattgttc agactgtaat cgtgcatctt
    10981 ggacatagcg gataagtaag ccatatagcg gctactttca aaaagctcct gtacgccggt
    11041 ttccagacgg tcagtaatca ctttcatgcg ttctttgctg ttcatgccgt ccagcatgat
    11101 cgggataacc gggggcggcg ctttgtcggg gctttcggat gcctccggct gctgggcctg
    11161 cggttcctcc cggtcagctt ccggctgggg gtagtgcatc acccggtatt cctccggcac
    11221 cggctggccc tcatggacgc gctgccattc gtcgccgctt ctcaccagat agccgtactg
    11281 ggtaaaaacg ccttgttcct gctgggcaat gtggcgtcca aactgcgccg tatcaatccc
    11341 ggctttccat tcctccggca tatccaccat gccggaacgg ttcaggtagt agtcgcccaa
    11401 ctgtccatcc cccctaacgt cgggaagatg cacatagtaa tccacatttt cggtgtagtc
    11461 gatgatctgg ccgatattct caaagccgcc acggggattt tgcagggcgg cgttcagctc
    11521 tgcgatctcg gcggcgtcaa gctggcccag ccgggcggca aggaaattca gctcgtccac
    11581 gtcggcggcc agcaccatgt catagggaat ggcaaggtgt ctgtcctccg ggcaggaata
    11641 cccgttgata aaaaaatcct gcggattgtc ggcagtaatg ccaacatccc gcagggcggc
    11701 ttgcagcgtt tcggttgtgg tgggcaaatc cagccagacg ccttgtttgc cctgttccaa
    11761 aaactgccgg ttatggactt ggatagaaaa tacttcgttc acgcggtcac tcctttctct
    11821 gttctgtttc aaaaaatagg gggttgggga cttccccaac aagcaaaaat ccccggcccg
    11881 gcagggacgc ggccggggga ttttaccggg agtgtagcca cactccctat gcttgctgtg
    11941 ttactgtcct ttagctcctg tgttcaatgc ggtagttgac gtacttgccc ccggtatcag
    12001 ccaaaagcac cgtcccgtcg taggttttgc cggtctttac ggaatagagg ccctttacct
    12061 tgactttgcc ggttttgagc agcgcagcgg cgatcttcgg ggtgaacgct ttcttgcgtt
    12121 cctcaaaaaa tcggtcattc ttccacatga cgaactggca ggcccggttt ccgcagtagt
    12181 agtttttctt gccctcatag acagcctcac cacagcgggg acacgtcccg ataggaacac
    12241 gctcggcctg aaacaatttc tgcttttcct cactgataca ggaataggtc tgcaccagct
    12301 cccgcgcctg cgtctcgatg ccgtccataa attcgtcagg atcggcctgc cccttggcga
    12361 tctcggtcag ccggttttcc cattcggcag taagctgggg agaagtaagc gcctccggca
    12421 gcaccacagc cagattgatg ccgtccttgg tgggaatgag ctgcttgcct tttctctgga
    12481 caaagcccat ctgcaccagc ttttccagaa tggcggcgcg ggtggccggt gtgcctaatc
    12541 ccttgcgctc ggcatcctcc ggcatatcct cggccccggc ccgttccata gcggacaaaa
    12601 gcgtatcttc ggtgtagggc ttgggcggcg tggtgaaatg ctcggtgaca gaggcggcca
    12661 cgtctgcaaa ggtctggccc tcgcaaagct ccggcagctc ccgcaccgcc tcggcgtcct
    12721 cgtcggcgtc ggtctttaag gaagaacgga accggcgttc aatctctttc cagccggggg
    12781 aaaggacggt cttgcccttg gcggtaaact cctgcccggc acaggtaaat gtggcggtga
    12841 cggcctcata gatatgcggc ccggccacgg cacataagag cttgcagcag atcagggaca
    12901 gcagcttttt ttcactctcg gccagagtgc caaagccctg cttcacaaat tcagcggtgg
    12961 ggataatggc gtggtggtcg gacacctttt tgctgttcag gatgcggtca aactgcgggg
    13021 aaaggttcag gcccttggca aacggcacca gctcccacag gccggacaca aggctttcca
    13081 cggcgggcag catatcgtcg gtaagatatt gactatccgt tctcggatag gtcagcagct
    13141 tctttagata cagtgcctgg gcatagtcaa gggtctgctt ggcggtatag ccgaaaagcc
    13201 ggttagcctc ccgctgcaag gtggtaaggt catagagctt gggcggctgt tccgtctttt
    13261 tctcccgcgt cacggaaacg caaacggcct gcgcttttgc acaagccgcc ttgacggtgt
    13321 ttgcctcggc agcgtcggaa aagcggctgc ttaccgcgtc cgcgccgccc gccgtgatat
    13381 ggacgatgtg atatttctct ttcttgaaat cggtaatctt ggcgtccctg tccaccagca
    13441 tttttaaggt cggcgtctgg acgcggccca cagtcaggtt cttgtggtac aggacggaaa
    13501 aaagccgggt ggcgttaatg ccgatcagcc agtcggcctt ggcccggcac agggccgatt
    13561 gatacagcgg atcatagtcg ccgcccggtt tcaggtgggc aaagccgtcc ttaatagcac
    13621 tgtcctccat agaggaaatc caaaggcgct caaagggctt ttggcatccc gccatctggt
    13681 agacgaaacg gaaaatcaat tccccctcgc gcccggcgtc ggtggcgcac acaaggccgg
    13741 tcacgtcggg gcgctgcatc agcgaacaga gaacgtcaaa ctgctgtttc ttgtcgtcgg
    13801 ggacgatgta ctgccatgtt tccgggagaa tgggcaaatc ctcatagcgc cattttttga
    13861 aacgctcgtc atagcgtccg gcgtcggcaa aggacaccag atgtccgatg caccagctca
    13921 ccagatagcc gttcccctcc aaatagccgt cgtgtttctc cgtggccccg atcacgcccg
    13981 caatgctgcg ggccacgctg ggtttttccg cgattacaag tttatatgtc attcctcgtc
    14041 ctcctgttca ctcacaaaat cagcttccgg gtcaaactcg gtttcgggtt cctcgtcctc
    14101 caaatcctcg tcctccccaa aatcatactc gcccaaatcg tcgctgccgg tggtttcagc
    14161 cttgggcttt ttgaacttga aatagtaaag ggccgcaccg ccgcccgcca gcgccaccag
    14221 caggaacacg atcaggccgc ccgcgccgcc cttgggttct tcctccgggg cttcgggttc
    14281 ctccggctgg ggttcctgcg gctccgggcc gctgcactcg gtcaggttgg ttttacatac
    14341 cgggcaattc gtgttcacag ccccggcctt gcatttcgtg gtacaggagc agacagcagc
    14401 gggggtgacg gtttccccgt cctccatcag cgtcagcaga tcggcttcat ccacctgatt
    14461 taagaaatgg acagtttctt ttccctcgtc gtcccggtca ataatcagat aaaacacatt
    14521 gccgtttttg gtttccacgg tgataaactg cttgccattt ttcgcggcgc tgccgatgtc
    14581 gtcaatcagt gacaggttcc cgtccgggga cagggcggcg ctggccccgc cgccattgaa
    14641 cagttccagc agagaagcaa tttcctccgg gtcaatggag agggaaaaac cgccctcgcc
    14701 ggtagccgct acttcctttt cgctgacggt catatagtcg gaatggacat agccgatgcg
    14761 ctccggcaag agaattttaa tccagtcgcc gtctgtgccg actacctcaa ccgtcgtccc
    14821 attgggaagc tgggtgaacg cggtcttgtc aagccccgcg ccggtgcgga cattcagcct
    14881 gccgccgttg gtggtgacgg tgccggtctg ttcaggttct tttttgtcgg tgccggtggt
    14941 gatctggatg ccgtcgtccg tggcggcaat gctcaccttg gaaccgaaca gcgcggaaaa
    15001 caggtcggtg atgtccttgc cggtcagagg gccggtatct ttttcggcct cggtttctgt
    15061 cccgtccgtg gcggtgccgg tttcgtctgt ttcaggctcc aaaccgcccg tagcgggttc
    15121 ggttgtagcc tccggcagcg gggccggtgc cgggtcattg ccgcctgcaa gggcggtaac
    15181 gggaaaggcc gtgacgcaga gcatcacggc cagaagtgcg gggagaatac gggaaatccg
    15241 tctggtctta ttcttcatgg gcattgtcct cctgttcatg gtaaggggtt gcggcagggg
    15301 cggcctgcat tgcgccgctg cgtaagaacg cggcaaattc ctccggcttc atgttcatgc
    15361 cgcgcacaag ctggatgatc tgcaaattct cctgttcggt tttctgcgtt tccagctccc
    15421 gaagctgttt ctgaaactcc gcgatcttag acttggtttt ctggatgtcc ttttcgatgc
    15481 ggtcaagttt ggttgccata attgaaagct cctttctggc gttaaggtaa acgcccgtaa
    15541 ctgtaaaaat gggattgcca gtagtttgaa gtaaggttcg tgtaggaaat cgggttcccg
    15601 caatgcagca tcacattgtt gcccacatac aggccgacgt gggacacgcc gggggtgtcg
    15661 taggttccct tgaaaaatac aaggtcgccg ggcttggcct gcgaactgga aacaggggtg
    15721 cagatgttat ataggccctg cgcccccagc cgtcccacgt tccatccact atgattgatt
    15781 acccacgaaa tgaagccgct gcaatcaaag ctggtgctgg gagagctgcc gccccacaca
    15841 tagggataac cgatatattt ttccgcctct ttgagcatgg cggcaaaggt ggcatcctcc
    15901 aaagcctccg ggggtacgtc gtagtaggtc ggcggcgtca gggtggaggc gttggggtac
    15961 tggctgccgg aaaacagatc aggccggttt cctagcgtgg acatatacag ggaatagaaa
    16021 cttagcgttt cctcgtccat gatatagact ggcacatggg acaggtcgaa gttttccagc
    16081 gtgacgtagc agatgtaata gtcatacggc acttggtagg tgtctgtatg ggtgttgccc
    16141 tccgcgtccg tccatgtatc ggtttcggtg cggtaacggg tttctaccac gacgttttct
    16201 gtcaggatat attgcttgtc aaagagcatg gacaaatcac cctgtacctc cccgatggta
    16261 aatatcccgt catggagcgc cgacagaatg gaaatcagca cataggggtc atggctgatc
    16321 tcgtcaaggt caaaatggta ttcgtcatag tcatgggtgg cctcgtagtt gtccagatag
    16381 tcttgcagct cggcttccag ctccgcgtag gcgtcctcgg cggccaacat atcgctatcc
    16441 tcggaaaagt aagaggacgc cgtgatgccg cttccggcgc tgccgaacat ggcggtgcag
    16501 gactgtaagc cgccgatgat aagcagcatc aaaagcccca acacaaggac ggccagcgcc
    16561 cctttccagt ggcgggccac aaaggaggcc gcccgttcac tttctttggc tgcggccttg
    16621 gccgctttcg tggaggcccc ggctgtcttg gccgcgccct gtgccgccgc gcctgcggtc
    16681 tggcccgctg tccgggcggc cttggcgtac tggcgcttga tctgctgttt ctgccagaac
    16741 cgggaaatgg ggttgctggc cgcctgtgcg atctccgggt tatcccgcaa agatttctgg
    16801 tagaaatact ccgcgttggc agacaatagc ctgcgctcgg ccttttccgc gtcccagtag
    16861 ggtttcagcc tgtggtgccg gatggtgctg cggatggctt ttccggcctg ccgctcggca
    16921 agttcctccc ccttatggcc gccctccaca cccacgtttt catgctcgac ttcatggatt
    16981 ttcccgtggc cgaatagaaa aacctcggaa acaggacggc ctgccggatt gggtttcagg
    17041 gagggaggcc ccttgtcctg tttgtcaaag tgcaggacgg tctttgcttt gccgcttgcc
    17101 tcgtcaaaga cggtttcctt gaccgggacg cgcttcttgg ggatggcggc gcgggcctta
    17161 tccagcttat cggcccgttt gtccgatttt cggatatagg gctgtaaatc cgggtcggcc
    17221 cgttcttcct ccgacagttg gagccgggcg gatggccggt gcagggcagc gtccccctcg
    17281 gcggccacag ccccggcctg cttcgcggct ttcttggcct tatggccgtc atggatggcg
    17341 tcggcccgtt ccagcagctt atcggcggtg ccgcccggcc ccggcgcgga caaatcctgt
    17401 tcggcctccc ggctggaaat gttctccacc tcgccggtgg cctgattttc cagcgtcagc
    17461 ccgtcgcggg tcatggcctg cgtcactttc gccttggctt tcagctcttt catggtggtt
    17521 cacctccttt gtggcgtcgc cgggtcatac tcccgccacc tcgtttaagc gggtcgtcat
    17581 aatcttgtaa agctgggtgt tgtgcgggat ggggttggta aagggcagca ccacgttttc
    17641 aaagatcaaa aggccctcgc cgggttcgga attgtcgatg tacttctgct gttcggtgga
    17701 caaattcaag cgttccgcaa ggatttttct atccccggac gcctgattga gcagcgtaat
    17761 gaagtcgctg ttttcaagga tattttcaat ttccggggag gaaagcaggt ctttgacgtt
    17821 ctgggtggca cctgttggaa tgccgcccca tttcctaaac cgcttccaaa tctccgcaga
    17881 ataggccgcc gtctgttcct ctttcaaaag caaatggaac tcgtcggcaa aataccatgt
    17941 ggcctttcct tggcttcggt tggccgtgac gcgcccccat atctggtctt ggacaatgag
    18001 catccccagc tttttgagct gcttgcccag ctccttaatg tcaaaggaca caagccggtt
    18061 gccgatgtcc acgttggtac ggtggttaaa tacgttcagg gagccggaca catacaggtc
    18121 aagggcctgc gccacccggt cagcctccgg gacatgctga tctaacaacg ctttgtggag
    18181 gtcggacagg atcggcatat tctccgggcg ggggtctgcc agatagggcc ggtagatcac
    18241 gcgcacagcc cggtctatca cggtcttttc aatggcttcc agcccgttct tgccgcccat
    18301 gacaagctca cagaacgaca gcacaaagtc ggatttcagg gccagcgggt tatcgtcctc
    18361 cgaataattc aggttgatgt ccagcgggtt cacaaagtcc ttgctggtag gcgacagccg
    18421 gattacctgt ccgtggagcc gtttgacaag ggggtaatat tcggcctccg ggtctgaaat
    18481 aaaaatatcg tcaggcgtcg ttaagaacac actgacgatc tcggatttac agctcatgga
    18541 tttgccgctg ccgggggtgc cgagcttcaa ggcattgggg caacgggcct gcttcctgtc
    18601 cagcataatc atgttgcggg acttggcgtt gatgccgtaa tacatggccg cgccgccctg
    18661 aaaaagctcc tgcgtcacaa aggggacgaa cacggccacg ctggaagtcg taaggctgcg
    18721 ctgtatcttg atctgattga cgcccagcgg caggctgctt gcaaggccct gttcctgctg
    18781 gtagtccagc cggacaaggg gacagttatg cttttgggcc acgcctgcgg cttggaacac
    18841 gtcgttattc agcttttgct tggtgtcggc cacgttgagg acaaggaacg tcaactggaa
    18901 cagccgttca ttgcgggtct gcaactttgt cagcagcttc ttggcgtcct cgccgtaggt
    18961 ggcaaggtcg ctgggaagaa tgtccatatc gtaaccgctg cggacggcct ttttctgctc
    19021 cgcaatcttc atagcgtcaa ggtcggtaat tttgcgcttg atggtcttga tcgccttggt
    19081 ctgttcaatg gcctgcacat ggagattgac taaaacaccg ctttccgtat tgaggaagtc
    19141 ggccagcata tcgtccgaca gctccgggga caggatttgc aggaaactta ccgcgccgta
    19201 tttgccgccc atgccgaatg tctttgcatt gccgaagcac agggaggacg gggcaataaa
    19261 atccttggtg gagaggccgg acggggccag ccacttccag tcaaaattga atatctcgcc
    19321 gtccgggtgg tacacgccat gcagcaggtt cagccgttcc cagccgtcca tcacatgggc
    19381 tacggccccc atgctcttga aatagcctaa caggtcaaag ccgatgcgct tcaatctggc
    19441 tcgcgccgct ttcacgcttt cggcctcgat ggtgaatgtc aggtatttgg tctttaccat
    19501 gccgttgttg ccgtttgcaa gctgcttttg cagaatgccg gtgtactcgg cccggatgtc
    19561 gtcgaagtcg tccccctgcg gcgcgatctc aaagctcttg gcgtactgca ccgggtcaac
    19621 tttccgattg aggaaagaaa actggatatg aatggaggcg tccacatagt tatacaggtc
    19681 gcacagtttt tcaaagatag ccgtctgcgt gtccggctgg gcaagctgat agctgatgtc
    19741 cgcaaactca atgcacttgg aaaagacgcc gggcgatacc cggcacacgc cgtccgggta
    19801 catgacctcg taggggatgc tctgctgcgc cgtgtggggc ctgccgtcgc ccttatattt
    19861 acggattacc gcgtcaattt cttttcgctg ggcgcgggtc agctttgttt tttcggtttc
    19921 tcttgggatt tcctttttgg acaatggcct ttacctcctg ttccatctgg tattgccgca
    19981 caagggcggt gtaggtgttg tcggtctgat agggacgctc ctttggctgg atgaagcagc
    20041 actggatgat ctgcataatc accacttcaa ggggctggcc gtgacgctcg tacatggcaa
    20101 gcaaaaagcc cggcagcatg gcaaatacca tgagcagggc cgccgcgctg ttggtaagat
    20161 agtcacgggt gagaaaatat aaggggacgc cgatcaccag cgccccggaa aaacagatca
    20221 gttgccgttt ggtaagccca aacacaacct tgcttttcac atgggttaag tccttgggga
    20281 cggttacata agccatttgg cacctccttt cacgtcagga taaggtcaca ttccacctcg
    20341 ttcccccaca cgtcccagcc gggggttttc tgtctggcaa acagctccac gcggggcaaa
    20401 tcgcccgcca gctctacgat tttttcacgg gtcacatccg gctttttgct gtgttgttca
    20461 atcgggctga taataaactg atggacagag gcagaacgac gcttcggctt gccgtgtgtt
    20521 gccagcaggc agatttccgc gtttcccctt gtccagcggc ccagcccgta aaaccatgta
    20581 aggcttttac ggttctgttt cagccataca aaagccacgg tcttgaatga aaagccccac
    20641 gctttgatta accgcagagc ttccggcagc atggggaatg tggcccacaa aaacagcagg
    20701 cagtctttgg acgccagccg ttccaccggc agggcgcaca gctcgtcaat gctcatggtg
    20761 gaatagtgat tttccgctgc gccctgtacg tttttgcagt cataccgcca cggcgggtca
    20821 gcgtaaatga tgttgtacgt tgaaataagc aagttcctcc tgtgtatgct tggattttgg
    20881 gatcaatgcg ccccgaatac ggcctttgat atactgccgg tcttgaacag gcagaagcac
    20941 agcagcaccg tatagcccat acagccccag attgcgccgg atatgtcgga agcggaaccg
    21001 atattctgta tcagcaccgc gtagataccc accacaacca tgattaaaaa ggcttggaag
    21061 cccagcgcca gcagggattt cagataccct tggcccatgc cgcgccattc cgcgttgccc
    21121 attgtggcaa gggggatagg ccccagcgac gtgacaacat agatttccac catgcggcca
    21181 taggtcacaa ggaaaatgca gatggaaagg atattcatgg tgaggcccac aacaagggtt
    21241 tggaaccaca gccccaaaag agcgcccacg ctcatagcct ccaattggct ttcaaggtct
    21301 ggtatcaggg tgttaaaatc aatggatgtc tgcccgataa tcacaccggc gctttgattg
    21361 acgacgcttt gggtggcgtc gaatacgccc atgacgatgt tccatgtgtt cgttacgatc
    21421 aggattgcaa acgcggattt gaatatccag cggaagaaca tggagctgtc aaagtcgtgc
    21481 atattgttct tttccgttat catctggata agctcatagc acatgacaag ggcgagtatg
    21541 gccccggcta tggggacgat cacggtttcc gacaggctgt gcagcatatt gaaaataccg
    21601 ctgttccatg cctgcggggt tcgtcccacg tcggcggcga tttctccgac tttggcattt
    21661 accgtatcga acagcccgga caggttgccc gtgataccgt ccatgaggat gccttttatc
    21721 cattcttcaa tgatttcact aatcacgggt agcacctcct ttcccgcgcc ctcccggtct
    21781 ggccgggagg acgcggaggc gattagttaa gggtttggac agggacaaag gccaggatca
    21841 gcccagcagc ccggacagca ggggaaccag agtgacaccg atcagggcca cgccgccgcc
    21901 agcgacaagc tgcttgacac cctgcgattt tgcccccgga ttgtcctgcc cgtatccctc
    21961 caacagattg atgccgcccc atacgcacag agcgccgccc agtccgatca cgatagtctg
    22021 caaagtagta actgcggaat taaaaaatgc cataaattta cctctttctg ccggtttccg
    22081 gctcataatg attttttgat tttgttgttg gaatgaaaaa agccgctgtt tattcagcgg
    22141 cggcgtcctc gtccgacagg tcgatttcat acaggtcgaa tacttcatcc tgcgttacca
    22201 cttgctgccg tttccggcgc gtgatagtca aaaatctgtc cacgtcaaag gtattcttct
    22261 tgtcggcgtc ggcaaggtat ttgtaccggg gatgcttggt aatatcgaat ttctccgata
    22321 aaaacgggcg cacaccccgt agctgcaaga tacacttgcc gccgtccatc acggcaattt
    22381 cgtcttggga catcaactcc tttcctaatt tttgatagtt caatccgtgg gacgtttggc
    22441 ttccccggtt ctcgctggtg ttgaaactgt caatcgtttc tttccccagc gcatcggaaa
    22501 tctccttggc atttttgccg cgcccactaa ggaaaagcac actatcgcag ttatccgaaa
    22561 tgatctcggc ggcgtccttg taaatcgctt ttagctgcga ctggctctgc aaaataatag
    22621 aggccgatat ttcacggctt cgaatggtgg caatgagttt atcgaaattg ggtatttggc
    22681 caatgttggc aaattcatcc aaaatcaggc gtacatggac aggcagccgc ccgccgtaca
    22741 catcatcagc ccggtcacaa agcaggttga ttagctggct ttggagcata gccagaatga
    22801 aattaaaggt cgtgtccgta tcgctcataa tgagatacag caccgtcttt tcgtccccga
    22861 tggtgtccag ctccatttcg tcgtcctcca tcagctcgcg tacctcccgg atgtcaaagg
    22921 gcgcaagtct ggcaccgcat gaaatcagga tggaagaacg ggtcttgcct gcggacagca
    22981 ggaatttctt gtactggcgc accgcaaaat ggtcggggtc tttctgctcc aaacgctcaa
    23041 acatgagatc gacggggctt tgaaactccg ggtcgtcctc gcgggcttca ctggcgttta
    23101 tcatttccag cagcgttgag aagttcattt cctccgctgg ggcctcgtac cagatatagc
    23161 cgatcagggc cgaataaaac aaccgttccg acttcaccca aaaatcctcc gctgactttt
    23221 ccccctcgcc cttggtgttg gaaatcaggg tatttaccag ctttagcacg tccttttccg
    23281 aatggatgta gacaaagggg ttgtacttca tggacttgga aaagttaatg gtattcagga
    23341 ttttaatgcg gtagcccgcc tgctggaaca tcctgccggt ttccagaacc aaagtgcctt
    23401 tcggatctgt caccacaaac gaagtcggat agtccgtggt cggaaagcat tgcatcaggt
    23461 tgggtttcac gaaaaatctc gttttccctg aaccgctgcc accgatcacc agcacgtttt
    23521 tattccgcgc tgtcttgggg tctttgggcc tgccggtcat ggtaagccgt tccgtttggg
    23581 tgagcaggat attattttca aacttggggt cgatgtaagg cgctatgtct ttggccgtcc
    23641 cccagcgggc gctgccatac tccatgcctt tgcggtattt cttcacgttc ttgctcttgc
    23701 agtacaccac cagccgcacg atcacggcac cggcgatacc gatacacagg tcaaacaggt
    23761 gcaggcttgg cagcgggttg gcaaaagccg ccgcaaaacc atccgcaaaa tgcaggagct
    23821 tccccgacag gtcagcacca gcggccagcc ggtacgcctc cccgcatttc gcaaacaggt
    23881 acacaaaaat cagatacggg aggttcagga tcagcaattt ctttacgtca ggcttcacag
    23941 ttccagcccc ctttccttga ttttctcctt ggcccgctgc ttggtctggg ccgccgcctt
    24001 ttgcttcaca gcggacaaaa ccttgcggat agagggccgc tgctccttgg aaagcgtctt
    24061 ggcggtaaac tcccgaaacg ccagatcaag gttatctgca tccttggcct tgaaaatcac
    24121 aacgtagtgg ggcggctggg tggtcttatc ttttcgtaag gtaaaatcca cgccgtattt
    24181 cttggcgcat ggcttgaacg ctccaatgtt ggcctcggtg atctcaatgt tggagagatc
    24241 agccccatgc tttttaagct gctttaggct ctgctggccg tggtgcagcg tgtttttgcc
    24301 cttggcccgg tgttgcaggt actttttcag cgccgcttgc agcacttgag ccgtgagctt
    24361 gcccgttttc atggacaggg caatggtctt ttgagtaact tcttcctgca atcgcatccc
    24421 tccttgtcag aaagtcaaaa gcggcgtcta aggcggaacc accagacgcc gcaggtagat
    24481 ataggtcaaa tccgcacccg caggccggaa aggtcgtcgg cccggatgcc gacaagatac
    24541 cagttttcgg ccatttcaat gcgggtgggc ctccatttgc cgccgatcat cacgtcgaaa
    24601 cactccccgc aatgcaggcc gccgtaatag tcggccagat caaagcggat gtcgtaacgg
    24661 tctgttcgct cgtcaaaaat caatgcgccc tgcttcatag ggtcgtcctc cttttctcgt
    24721 tacaggcttt cgccctcgcc gcaggaatag tccggcaggc cggatttagg ctttccccaa
    24781 tcgggctgtg ccatgtcgtg cgcgacaagg gccgtgtagt agttggaaat ggtgctgggg
    24841 gcgttgaaca gcatagcccg caggtattgt ttgatattgc ggactttcgt tgtgttctcc
    24901 cgcagacagt caaggacaaa gcggatatgt tcgccggtca gcttcatgta cttggatttc
    24961 accagctcgg cagggtagtc gtccccggcg acacggattg tctttcgggc ggtgcagacg
    25021 gtttccagca tcaggtctac gatttcgtca agctgttcac ggtcaatgct cctgtcctga
    25081 atgagatagg catactcgat gttctccaaa atgatttccc gataaatccg tacagcctca
    25141 tcctttcctg tccttttccg ttccggcggc gtagccgctg cctcgccctc cataggggca
    25201 ggggtcgggg atgggatagg aatggaatgg gtactttgtc catctgtaat tattttttct
    25261 ttttttgata agtcagttct ttgtacttct ttatttaatt gcgttggatt ttccgacgcc
    25321 ggattatccg atgtcggatt ttccaacacc gggttatccg atgttggatt ttccaatacc
    25381 gggttttcca ataccggctg ttcgtagatg gtatagacca tatcggccat tttgcctttt
    25441 gcgtcacggc cctgctcacg ccggatatat ccagcttttt caagctccca taccgctgtc
    25501 ctgatcgcgt cgatgctttc ccggttgata taggacagtc cttttagggt atagtcccaa
    25561 tcctccggca aggacaacat ctgcgacagc aggcccttgg cctttaaggt caggttctta
    25621 ttccgcaggt ggtgatttga catcaccgta tagcctttgt tcttttccac acggaaaact
    25681 gccatagcgt tgttcgctcc tttcactgtg aatttgaccg ctgcatagtc ggtcagcttg
    25741 cccggctgat agggacactc tgcaaactgg cagaattgat atttccaatc cgggcggtaa
    25801 aatcggcagg tgccgcaatc ctccggcgcg tccgccccgc cgccgtcgta gtgatcgaaa
    25861 ccgggctttt cctgcatcag ccgttcaaac gcccggtcag ggccggaagt aaatagcatg
    25921 gcgtcgcctc ctttctctat ttgggcataa aaaagcggcg tcctgttctc ccatgacggg
    25981 aaagaggaac gccgcgttta cggtgtcgta ttcaatttat gatgtgccgt atttggggtg
    26041 gcgggtatta gcagtatggt tcagcatata tgcggctcaa tccgaaagta gtaaattggt
    26101 agtaaattga gttctcatat cctgcgaaat gcccgtattt ccggggcttt cagcgataac
    26161 ctaaattttt ggaataaaac tttcattata ggctactgca acatccatcc aatcagcaat
    26221 ctctttttgg atctcaggtc gtttccagta gccaacgacg attttatttt gtttatttaa
    26281 tcgagcattg atgaacccat attcgcggtc accatgagcg gattgattta aattcatgaa
    26341 atccatatcg atcgttttcc aaggaatgct ttcgttatat tgcgtagcta aatgaagcaa
    26401 tggtttttgt agaagctttg tcccgcggat ccacattttt gctggtgaga aagtatgcat
    26461 ccaagtgatc acacctgcta cttcttctcg ataattgacc tctttcatga tttttgtgat
    26521 cgtatcagca gtaactgcta attcttgaag gcggattgga taaggcaatt ggccattttc
    26581 attcagttca tcgaccattt cttgtgcatg tttctttact tctcgcagtg cttcttcccc
    26641 atataaatgc tgtgatccta caacaaacca aaattctttt tctccaatat tcaacataat
    26701 tagccactcc ttttatttgt tttgtccgta atacgcattt tttccgtgtt ttcgtaaata
    26761 atgtttatct aaaatccgtt gaggcagctc ttctgaaaat gaattgagct gtctggtaaa
    26821 taaattcatc ttgcatactt catctagtac gactgcgttc attaccgcac tttgcgcatc
    26881 tttcccccaa gtaaatggac cgtgtccgtg taaaagtacc cctggaattg ctaagggatc
    26941 aattttgcgt tctttgaatg tttctatgat aacttttcct gtttcatatt cgtacccgct
    27001 gtcgatttcc tgttgtgtca aaaatcgagc acaaggtacc gatccataaa aagtatctgc
    27061 atgcgtcgtc cccatcgcag gaacatccaa tcctgcttgt gcccagatcg tcgcccatgt
    27121 cgagtgagta tggacgattc ctccgatttg aggaaatgaa cgatataaaa cagcatgagt
    27181 aggcgtatcc gaagagggat tgagtttacc ttcaatgata tgattgtcca agtccacaac
    27241 gaccatgtcg tctgctgtca tctcttcata actgactccg ctaggtttga taacgaaata
    27301 tcgtgtttca ggatcaaaag cgctcacatt tccccatgta tatttgacga gtccgtgttt
    27361 aggcaagtca agatttgctt ggtatacttc ttctttcagt tgttccagca ttttctatca
    27421 ctcctttttc ttgcaattga tgaatcgctg ttttttcaat gtcgatcccc tcacggtaac
    27481 gtctcataaa tatctgatag cctgctattt cttctgctga aggtgtgatg gtgattcctt
    27541 ggtcttgtcc aaacacgtct tctgccaaga agttttctaa agacttccca tgtcgatcca
    27601 tcatataggc tgctaataac gcgattcccc acgctccacc ttctccggct gtgtccatca
    27661 ctgtaactgg tgcctccata gcagatgcta gaatccgctg tccaacttct ggtgttttaa
    27721 aaatcccccc atgccccact aaacgatcaa tttggacatt ctcagatttc aatatatcca
    27781 ttccgattct cattgcacca aaagcactgg ctaaatggat ccgcatgaaa ttcgctaaat
    27841 caaaacggct acctggtgta cgtacaaata atggccttcc ttcatttact cctgtaatgt
    27901 tttcaccgga aaaataacca taagaaagta atcccccgca atccgtatcg ccttctagcg
    27961 ctttgttgaa taataccgtg aataatttat ctctgcttat ctccataccc aaagaattgg
    28021 taaattcttc aaaaatactg acccaagcat tgatatccga agagcagtta ttcgtatgaa
    28081 ccatgcctac tagttctcct gaaggtgtag tgaccatgtc gatttccgga tggacatttt
    28141 ctagttcttt ttctaatacg atcatagcga aggcggaagt accagccgaa atgttccctg
    28201 tccgtttggc tacactgttc gtagcgacca taccggttcc tgcatcccct tctggcggac
    28261 aaaccgggat tcctggtctc aaatttcccg tagggtcgat cagataagcc cctgattcgc
    28321 ttaacttgcc agcttcttct cctgctacta gaactttagg caaaatgtga tccaagtcaa
    28381 tcgctacgtg cgctttttcg attaactgac caaaaatatt tttcatttct tgatggtagg
    28441 ttttcgttga aggatcgata ggaaacatgc ctgatgcatc tcctacgcct agtactttct
    28501 caccagtaag ctgccaatgg acataacctg caagcgttgt caaaaaatta atttctttca
    28561 catgttcttc tttattcaaa atagattgat acaaatgagc gatactccat ctctgcggaa
    28621 tattgtattg aaatttttcc gttaatattc gttctgcttc ttcagtgatt gtatttcgcc
    28681 aagtacgaaa tggtactagt aactgttcct tgctatcaaa tgctagataa ccgtgcatca
    28741 ttgcactgaa cccaatcgac ccgattgtcg taagattctc gccatagatt tcgaatactt
    28801 ctgctgccat ttttcggtaa ctgacgcgca gtcctttcca aatatcttct aatgaatagg
    28861 tccagatacc attttctagt ttgttttccc actcataact accagcggca ataggagtaa
    28921 aagatgaatc aatcaataca gctttgatcc tagttgatcc aaactcaatc cctaaagatg
    28981 tacgaccatc tatgacatct aacatacttt gcgcatttac ttccttcata atgatttaac
    29041 tccctccttc tcgtcaaacc tgatcaaacc gtctttttag tagcgcttac aatgattatt
    29101 gtatactttt gtacgtacat gtcaagcggt tatttacaag aatacaataa aaaacacttg
    29161 ttttgtacgt acataagtta taaaatgaaa ttgcttacaa aatctgatca accgccttct
    29221 cgaaattcta gaaggagaga atacttatga aaaaagaaaa aaaagtttcc tcatcgttta
    29281 tttatttttt tggcgcattt ggcggcattc tatttggata tgatattggt gttatgaccg
    29341 gcgccttacc ttttttacag catgattggg gtttggcagg taaagcttct ttgataggat
    29401 ggatcacgtc ttcggtcatg ttaggtgcga tccttggcgg ctctttatct ggtcttcttt
    29461 ctgataagct tggacgacga aaaatgattc ttctttctgc acttattttt atggctggtt
    29521 ctgtcctttc tgcatctgcc cctcacaatg gttcttattt tctgattgca gcaaggattc
    29581 ttctaggttt agctgttggc gctgcatctg ccctagttcc tgcttatatg tctgaaatgg
    29641 ctcctgctag actaagggga agattgtcag ggatcaatca agtgatgatt gcttctggca
    29701 tgctcctttc ctatgttgca gattatttgc taaaagtgtt gcctgaaacg atggcttggc
    29761 gcgtgatgtt agggctcgct gctgtccccg cgctgatttt atttttcggt gttttagctc
    29821 taccagagtc accgcgtttt cttatgcaat ctggccgctt ggaagaggca aaaagagtat
    29881 tgaactatat tcggacacca aaagaagctg aacaggaatt cgaacaaatc cagctgaatg
    29941 tcaaacaaga aaagacaacc ggaacttctt ggcatacttt gtttttagaa aaatatcgtt
    30001 ccttagtcat tgccggtatt ggtgtagcag tattccagca atttcaagga gctaatgcta
    30061 ttttttacta tattccgctg atcgtagaaa aagcgactgg gcatgcagcg agcgatgcat
    30121 tgatgtggcc gatcatccaa ggaatcattt tagttgctgg ttctttgctc tttttggtta
    30181 ttgcagacaa gttcaatcgg cgaactttat tgaaaatagg cggtagcgtg atgggacttt
    30241 cattcatcct accagctgtt ttaggaactg tactggatgc tcatacaaat tcgttgttga
    30301 ttctcttgtt tttatgtatt tatgtcgcat tttattcttg cacatgggct ccactgacat
    30361 gggtaatcat aggcgaaata ttcccactag ctgtccgcgg gcgagcatct ggcctagcct
    30421 cttcttttaa ttggattggt tctttcctag ttggcttgct ttttcctgtg atgactgctt
    30481 ctatgtctca agaaatcgtt ttcggcattt ttgggatcat ctgcttttta ggggttttat
    30541 ttattcaaga gatcgtacca gaaactcgtg ggaaatcttt agaggaaatc gaacaaagtg
    30601 catctaaaaa aacttatcct aaacgtattt ctatccatca ttcttaaaga ggttgtgaca
    30661 gaagtggtca gcttcaagaa ataagaagga accgcttagt ttccagtcac aaggaacaat
    30721 ttcaaaaaac agcttctcat gtttgatgaa tattgtgact tgtgaccagt tggtaggaga
    30781 acaccgttta gtggagaggt ggtgaaagaa acgttcagcc ttgagcgatg acaaaagctc
    30841 tcacaaacat tgcgacgagt aaccgtagga gcaataagag gcggtaaaaa cccgtctctt
    30901 atttttcgtt ttctttcact gtttcccgtt caactagtac aggttcatag agaatatttg
    30961 gctgtgcctg tcctttttca atcgttgtga cgatccaccg tgcggcatct tgtcccaact
    31021 tttcttttgg atgcgtcaat gttgtcaatt ttttattccc taaatggctt aaagaagaat
    31081 cgtctgttcc tacgatcgat aggtcatctg gaatatgata gccattttct gtcaacttat
    31141 ctagcattaa actagctatt tgatcgttat agcaaatgat gccatctaaa gcttgttctt
    31201 ctttcaaccg ttgtactgtt ttttcagcga ttgtttctcg tgtttccgtg gtgaaagtaa
    31261 tgatatcttc cgtcaaaaaa cgcacattat actgttcaca cgcgctgata aaacctttca
    31321 tcctgtattt tccttgaaga tcgtcgattt ttgtgatgaa caacagacgc tgatgtcctt
    31381 tttttatcaa atattctgct gccataaaac cgactttcac atcatctaaa caaatataag
    31441 aggcatttaa ctcttcataa gcagcattga tcatcaccat cggaatgcct tgttctctca
    31501 aagcaacata aagtgctaag tttgggttat actgattact tttcgtaggc tcaacgatca
    31561 aaccatctac cccataagca atcattttct tcaaacactc tttctccaat tcgtgatcgt
    31621 tattcgtact ggctaaaagc aatgaatacc cagattctcg taattcattt tcgattcctc
    31681 ggataatcga cggaaaaata taatcagaga gatatgtggt gatgacaccg atcgtttttg
    31741 ttctgttcaa tggtgttttt tctttcttat agctgtcatc tacatatgtg cccgaccctt
    31801 tttctttacg cagatagccc tcatttacca acacagcgat tgcttgccga atcgtatgtc
    31861 tgctgacgtc atatatttct tgcagcttga attctgaggg aatggttgta tttacttcgt
    31921 attcctcacg aaggatcttt tgttttatct cttctgcaat atcttgatat tttgccattt
    31981 gccttgcccc tttttatttg tacgtacata tatcataacg agtctgcaag gtttattcaa
    32041 gcaaaaatca aggtttcttg tgctcataag gcatacggaa caggttcacc aaattctgga
    32101 aatccttgct cattccacgt aaagcattgc gcgttcgcgt gccgattagg attgtctaac
    32161 ggatcccctt tttggttctt ctctggtcga gcatgataga tcaaaatatc aaagcacccg
    32221 tcttctgaaa ccgtaaacga gttgtgacca ggaccaaatt gcttattttt ttcagaagaa
    32281 acgaatactg gctccgataa tttatgccat gattctttta ataaaagatt cttgtgctca
    32341 tcagcccaca gcaggcccat ggcatagttt tcatctgttg cactaccaga atacgtaatg
    32401 aatattttcc cattgcgagt aaggactgct ggaccttcat ttactgaaaa accccgtttt
    32461 tcccattcat actcaggtat agaaaggagg atctgcttcc cctttaacgt ccaaggatta
    32521 tccatttcgg atagataaag attagaattc ccagtaattt caggatcttg ttgggcccac
    32581 acataatata gctttccttg agaagagaaa gttgttccgt ctaaactgaa agtttccctt
    32641 ttcgttttta tttgtccctt ttctatccaa tctgtattca ttggatctat atgctgattt
    32701 tcaagtacga acatgcggtg atgatgagat tcatcacgta tagttttatg attgcttgca
    32761 gcaaagtaaa tataccattt tccattgata taatggactt caggtgccca gatcaattca
    32821 ctcatcggtc cttcttcatg agcctgccat acaatcaggc ttgcagcatg ttctaattca
    32881 ttcagtgact ttgcccttct taattcaatc acttggtagc ctggaaccga tcctgtaaaa
    32941 taataataac catctgtatg tttatatacc caaggatctg cccgttgccg aacgatagga
    33001 ttattataaa gtgtcttcat cataacaaat ttcctcacat tttctttatt ctaattacat
    33061 tccaagagta tttttctatc cctacattta attcgtcatg tttttctatt gaaaaatagt
    33121 ttatttcagg ttttattttt tctggttctt cacgtgtatt tttgtccaac agatcgctac
    33181 tggttaatgt gtagtgctct ccttgatcta gcaaattgaa atcatgttca atcgttatct
    33241 tttgttgttc atttgtgcga ttgatcaaga aaatagtcag ctgattgccg ttatctacga
    33301 tcacactgtc cacataagga acatttttta tcttttcaca atcataaaca gggcaaatga
    33361 tttgttgttt caatacttga cctttagcat atttcgataa gtgttgcatc acataataag
    33421 ttggattcct ccatgcttgt cctttagcat ctgtcagtat taaaggtatt gtgtttacta
    33481 acaatgattg acaactaatc tttacgcgat ctgcgtgtcg taaaatagcc agccctaatg
    33541 aaccaaataa taatgtatct tccatagtaa agtaacacca atcgatggga ctaccgactt
    33601 caaaatccct atgtcttttt tccggttggg catgtacatt ccattcatca aacgctagat
    33661 acattgtttt ctcacttctc aaatatcctt ttacataatc acaagtagca acgatttctt
    33721 caatttgacg atcgaatcga taagcttttg caaggtatgt cgctgtatca tcacgctcat
    33781 ttttaggagg tttagtcaag tcttcttcat catacttatc aatatagtta tgcaaagcta
    33841 aataatctac attctcataa gcttcttcta atgcgatacg atcccattca gggtacgatg
    33901 gtaaacgtgg cgtagagctt ccaactaaga ttgtttcaat tgaatcatcc actaacttca
    33961 tgacttttga ggcttcattt gctaacctac catattcata agcactctta gcccccactt
    34021 gccatggacc gtctagctca ttccctaaac accataattt aacgccaaaa ggcttttttt
    34081 gcccattttt tattcttaaa tcactccaga aggttccttt tggaaaatta caatattcta
    34141 ctaaattttt tgcttcatca attccttttg ttcctaaatt caccgtgaat atcggtttcg
    34201 cttgtacatg actgatccat ttaaaaaatt cgtggagtcc aaaagaattt ggttctatac
    34261 ctcgccatgc taaatcaatt ttttttggtc gttgttcttt cctacctaca gtatccaccc
    34321 aatcataacc agatgtaaag tttcctccag gatagcgaat gacacctaat tctaattcct
    34381 ttacataaga gaggacatct tgtctaaacc cgtcaccatc agctgtttca tggcccggtt
    34441 cataaatccc tgtatagacg acacttccca tatgctcaac aaatgagcca aacagccgag
    34501 aactgatctc tcctaccgta aaatcctctc tgatttttac tttcacttct tcgtccaaca
    34561 gctttcccta cctttcattt cctatagtca agataataga tacaagtttc catacgccac
    34621 cagcaaagat aaaaataaaa ggagggaaaa gaaatatgag cagtacaaat cctaataaca
    34681 atacgcaaca aagaatattt ctcaaaaaat caaaaaatac tttccatgca ctttgttcaa
    34741 aaatttgtaa aaatgtgata tctttgtttt cagaaatcac atatatccag gataaaagaa
    34801 acatggaata aataagtaaa actacatatc cactaattga aagtacgatt gtctgttctt
    34861 ctaataattg attaacaaga aaataaataa tgatcgttaa cagactaagg aacaagttct
    34921 taccaaaatg ttgctttatt ccttgaaaga aaaaataaat gatatttcct gatccctcac
    34981 ctttccattg cgccattaca taaaaaacag cacatgtagc aggcacgata gtaataacag
    35041 gcaaacacac gattacccag agaataccta ataagatgta atcagaaacc acaatcaact
    35101 gtccataaaa tttagtctca actattttca tcttcctcac tctttcattc cagacatagc
    35161 tgttccttga acaaaatatt tctgtgcaaa aatataaacc actagtaatg gcagcattga
    35221 aataaccgtt ccagccatta caggtccata aaaagaaatg ttctgccctg taaataaagc
    35281 taaccctgct ggtaaagttc tcatttttga actacttgtc aatatcaatg gataaagcaa
    35341 gtcattccaa ctattcatca gcgtgaaaat ccctaatgat aaaagagtag gtttgctcaa
    35401 tggcaacatg attttcaaaa aaatattaaa ctcacttaat ccatcaatgc gtgctgcctc
    35461 ttccaaagaa tctggtaaag taataaaaaa ggaacgcatc atgaagatac caaatgcagt
    35521 tgtcatacga ggaatgatca aacctgcata agtatcaagc atgccaagca aattgacttc
    35581 aataaataga ggaatcatga agacttggaa tgggatcatc attgtcagca aaacgaaata
    35641 gaacaaaact gattttcctt tgaaacgcat acgagcaaat gcataccccg caagagaatc
    35701 aaagaataca gaagtgatca ctgtccctcc agcaaaaatc actgtatttt tgatgtaatc
    35761 taataaaggg atcgttgtcc aaaccttaat atagttatct agggtatagc tatctgcaaa
    35821 tattctaggc ggaaatgaga taatgtcttt ttcgtgttta aaagaagaaa taatcagcca
    35881 caaaaaagga aacgcaataa ccaacaatat aactagcata ctaatcgttc gaatgatttt
    35941 tttaccacta actgcttttc gactattcac agtaactgta atcgagcttt ctggtgccat
    36001 acaggtttcc ctcctatttc atttgttttt ctttccgtga catgtacaca ttagtggaga
    36061 tagctatagt tgcaacaatg ataaacaaaa ccgaagacag tgctgatgca tagcccaatt
    36121 cataaggtgc tgtaaatcct cgactgtaaa tatattgaac agctgtttct gtcttaaatt
    36181 gtgggccacc agcggttgca acatatactt ggtcaaacac ttgcatcgaa ccaatcagat
    36241 tcgttaaaat acaaaaacct aaggagggaa cgatctgagg tagcgtaatg ttaaaaaact
    36301 gctgtcgatt tgttgcacca tccatttgag cagattcata caaagaggga gagattccct
    36361 gaatagcagt caagagaatc gtcatagtaa caccaaaatt cttccaaaca gtcatgatgg
    36421 ctaccgtcgg catagcaagg gttggatcgc gaaaaaattg agggatcggc aagccgactt
    36481 gtgaaaacaa gtaaggaatc gcccctacat tcggatcaag caacatagaa aaaataatcg
    36541 caattgacgt caatgaacaa ataactggaa tgtaaaatgt cgaacgacaa aaacgattga
    36601 atctggtatt tttagataat gctactgctg ctaataaacc aacgataatt tgtgttggcg
    36661 tttcgagtac ggtgaaatat aaagaatgaa tcaacgaatt aactgctcgt tcatcactaa
    36721 atatacgtgt gaaattttca aaacttacaa aatttcttga tgtaaagaaa atattcaaat
    36781 ctgtaaaact aatagcaaat gttcctaaaa gcggtatgac agtaaataat aataaaacta
    36841 aaaaagatgg agttaaaaac aaatacgcag cacgatcagg acgattccaa tatttggtga
    36901 atccgttcat atagtccacc tcatctttca ttttgataaa atatacaatc ttggtttaaa
    36961 cgaatctgaa taagatatcc gaacaatcgc tgtgattttt gcaataacac ctatctgcaa
    37021 atactaggtc gatcattcag atatctgtat cttgccaaca gtctatcctg tataaagttc
    37081 tgtactattt ttgcagtaat tgattgatgg catcatctgc atcgttcatg agtgtctcag
    37141 gatcttctcc tgctaataat ttttcaatca gtggattgat gatatcgtta ttgatcgcag
    37201 agatttgggt aaaacctggt aaatatggct ctgcatagtc catttgtttc gataattcag
    37261 atacaatagg atcatttttt acttcttcat cttttgctac tgaatccaag taggcaggaa
    37321 aaccattttc gatactccat tttttaccaa tttcagttgt attccaatat ttcaaaaact
    37381 catagacagc ttctttttta ttattatctg tacttgccgg aatggcatat cctacgccat
    37441 ctaaaatagc tgtttttttg tctgaatcac tcccttgtgg taaggtagtc acattataat
    37501 caatctcgtt tgctttcaat ccgttattta accatggccc attgatttca atggctagtt
    37561 gaccagcatt cattaaattg tccatttcgg cgccagaaat cgattctgga ccagtcttgt
    37621 ctacatggat cattttttgg atcgctttta acgttttaag gttctcagga gacgcaaatt
    37681 cagacttagt ataatcatcg ttcactaatt taccgccatt tgctaacatc cagttatata
    37741 acggtgcagt tccatcctta ttgaagacaa aacctgatac atttttacta ctatcagtta
    37801 attttggtgc catttctgtc aattcttccc aagtagtagg tggttgttca gtatctaatc
    37861 cagcttgttt aaacaaatca ttattccaat acatatacat gccttgtacc tgcatgggga
    37921 caaaatactg tttaccttct acttcaccta attcaatagc cgtttcttta aaatcagatt
    37981 tatctactcc atcatattcc cagaaatcgt ctaatggttg taccgctccg ttttctacat
    38041 actgtgcaaa atctggataa tttaatgcaa taaagtcagg tgctttttta gaagaaatcg
    38101 ctgtaggaag tttttcattc aaattggccc aagtcatgat atccatttca attttgatgt
    38161 ttttatcatt tgttttatta aattcattaa cgatttcttg caatatttca ccatcagatg
    38221 cagtaaaccc attccaaaaa gtcaaagtca cttctttatc agaatcagat tctgctgaac
    38281 ttccgccacc gcaagctcct aaaatacctg ttgcagctac caacaaagag ccaattaacc
    38341 attttttacc tttcattttt tgttcctcct tcatctgata aacgacatct gaccacattc
    38401 caagatagcg gtttcaataa acaattgatt ttcccatctt cagcttttac atttgttgaa
    38461 tgacttggtt ttacttgttc acttttagga agattagttg cttttgtatc aaatccactc
    38521 atttcagaaa aatcaatcac tccctctaat gataattcag acaagtgaac agttagattc
    38581 atttcttctt ctccacggtt aactgcaaat atgacaagtt ctttgtcatt tattacggct
    38641 atactttcta aatacggcac ttcttcaaaa tcttccgtcg catagtttgc tgaacgaatt
    38701 tgtgggctta acacctctcc ttgtccatac acagcagctt gcataaatgg ataatagatc
    38761 gtctgtctcc aagcttctcc gtttgtttct gtcctgattg gtgcaataac gtttaccaat
    38821 tgagcaagac aagcaatctt tactctatct gcatgtttca ataaagtgat taatagacag
    38881 cctaatagta atgcatcttc aaaattatag ttatcttcta gtaaaggagg agcaatctgc
    38941 caaggcgctg cttgctcatc ttgttccgct gaatgatacc aaatattcca ttcatcaaag
    39001 gacaggttga tctgcttctt actatgtttc ttcgccttga ctgtatcgca catcgctgtc
    39061 acttcagaaa tgaaccgatc catgtctaag gaacgtgcaa gataatgaga aagatcattt
    39121 ttttggttgc cataatattg atgcagagac aaataatcta tttgatcgta cgcttgatca
    39181 agaactgtta actcccaatc accaaatgta ggcatttgcc gattcgaact tccgcataaa
    39241 accacctcaa ttgaatcatc tacgagtttc atcgccttag ccgtttcctc tgctaaccgt
    39301 ccatattctt ccgctgtttt atgtccaatc tgccaaggac cgtccatttc attccctaaa
    39361 caccatgtct taattccaaa agggctctct tgaccattcg aacgccttaa atcactccaa
    39421 taagttcctt taggaaaatt acaatattcg actaaattac gagctgcatc tactccacga
    39481 gtacccagat tgacagccat attgatttcg gcccccacat gctgacacca gttataaaat
    39541 tcatgaatcc caacttcatt cgtttcaatt gtccgccaag ctaaatccaa ccttctaggt
    39601 ctatttgcta tgggcccaat actgtcttcc caacgaaatc ctgacacaaa atttccacca
    39661 ggatatctaa tcatgggaat gttcaattct tttaccaagt gaatgacgtc ttggcgaaat
    39721 ccacttttat tagctgatgg atgatctggc tgataaatcc cttcatatac tgctcgtccc
    39781 atatgttcaa taaatgaact gtacaaccgc ggatcaactt tgcctacacg cttgacacta
    39841 ctaatcaaca ttgtggcttc cataactgac ctcctttaat ttatacatga ataatacacc
    39901 tcgttgaaag cgttgtcaat aatataaatg ttaaacttac ttatttttta tcgattttaa
    39961 ttaaccaaaa tattaataat gattaaaaca aataatttaa tataactttt attaacaaat
    40021 atgttaatat ataagaaaaa ccttttaaga ggtgatatat atgcaactac aaataaacga
    40081 agaatcctta ccggtatatg aagcattagc tagtaaaaca aggatcaaga tcatccagct
    40141 actttccaaa aaaaagatga atgtcaaaga tttggcaaaa gaacttggag tgagcagtgc
    40201 tatcacgacg atgcacgtga aaaagctaga ggaagcaaac atcatcaaaa cagaaaaagt
    40261 aggacaacaa aaaatttcga gccttcgtgt cgataaaatc gatattagtt ttccagaaaa
    40321 aatattcaat gcttttgata cgaaagaaac atcgatacct attggtcact atacaaatta
    40381 tgcaattgaa ccaacatgcg gtttagctac gatccatgat tttatcggga aagtggatga
    40441 accacgctat tttatggatc ctcgaaggat ggatgcccgc attctctggt ttaccagcgg
    40501 atttgtcgag tatcaggcac ctaatttttt aaatacagag gatacattgg agatgttgga
    40561 agtttctgtg gaaatttctt ctgaatttcc tttttcaaat gacaattggc cttcagatat
    40621 cactttcagc ctaaatggtg tggagctagg aacatggacg agccctggag attttgctga
    40681 tatccgcggt aaatatacgc ctgattggta tccagataat cttaatcaat acggcttgct
    40741 gaagacgatc cgaattacca aacacttgac gaatatgaat ggagaaccct tgtcaaacat
    40801 cacgatcaac gacatcccta aagagcaaga cacttggcat ttgcgaatcg aagtgaaaga
    40861 cgatgccaaa catgtggggg gctgtacttt atttggtaaa ggattcggta attacgatca
    40921 ggatatcaaa ttaaaagttt actacagctg atatttttct tttctatttt ataaaacaaa
    40981 aacagccaat ccctatgaag ggacggctgt ttttgcttta ttgttatttc cagaaatcat
    41041 ccaaaacgga aattggcaaa tgtcgtttat gttgtgtctt gttccaccat gcttcgattt
    41101 tttcttgtgc ttgagcggga acttcttttc cttctagata atcatcaatt tcattatatg
    41161 tgacacctag tgcgacttca tctgcaatca ttggtttccc atcttcaaga tctgcagtcg
    41221 gaatttttgt gtataatttt tccggtgcat tcaatgcttg aagcaattgt ttcccttgtc
    41281 gtttgtccaa acggaataat ggcaaaatgt cggctccacc atcaccaaat ttcgtgaaga
    41341 atccagtaat gttttctgca gcatgatctg tccctaatac ggctcctgct ctttctccag
    41401 ctacggcata ttgcgtaatc atacgttgac gtgctttgat atttcctttg ttaaaatcgc
    41461 tgattcccac acctgcttcg cttaatactt gtacttgtgc atcaacagct ggtttgatat
    41521 ttactcgcag actaacatcg ggctggataa agtcaagagc agcttttgca tcttcttcat
    41581 ctgcttgttc accataagga agacgaacag cgataaattg atagttttca tcttttgttt
    41641 cttcacgcat ttcttccatc gttagttgcg ccaaacggcc tgctaaagta gaatcttggc
    41701 cgccgctgat acctagtaca aaagttttaa gaaacggata ttttttcaaa tatgctttca
    41761 taaagtcaat acttttacgt atttcttctt ttggatcaat tgttggtttg acacctaatt
    41821 cattgataat ttctgtctgc aggctcattt attctcctcc tatgcttctg tatgaagttc
    41881 tgctacgctt tttttcattc gttccatgat agccatttta tggttccagc attcagtaga
    41941 cagatcgact gggtattttt gcggattcaa gtctcgttta tactcttccc atagtgaatc
    42001 tagattttct tttgtatatt ctttgatttc gtttagtgca ggtaattcgt aaactctttt
    42061 tccttcgata aagatatctt gtaaaactgg gcgagctgta aagtctcgaa cagttttatt
    42121 gataaacgta tgcactggat ggaacatgaa gatagcttct tcttctcgag ggtcttcatc
    42181 ccataatgtc acgtagtctc cttcagattt cccatcaaaa tttcgagtga tgcgccacac
    42241 ttgttttttc cctggagtag ttactttttc tgcattgctt gataacttga tcgtatccat
    42301 catttttcct tcatcatctt cgatcgatac tagcttgaat actgcaccaa gggctggctg
    42361 atcataggct gtgatcaatt ttgtcccaac accccataca tcaattttag ctttttgcat
    42421 ctttaaattc aaaatcgttg cttcatccaa gtcattcgaa gcatagattt ttgcttccgt
    42481 aaatccggct gcatctaatt gttcccgaac acgcttagat atataggcca tatctccact
    42541 gtcgatgcgt acgccttgga agttgatttt gtcacctaat tctttcgcca cgcgaatcgc
    42601 acttggcaca cctgatttca acgtatcata agtgtcgact aagaacacgc agtctttatg
    42661 ggttttagcg taggccataa atgcttcata atcatttcca taagattgga ctaacgaatg
    42721 agcatgggtc ccgctagctg gtatcccaaa tatcttccca gcacgtacat tgcttgttgc
    42781 atctgctccg ccgatatatg ccgctctagt tccccagact gctgcatcca gttcttgtgc
    42841 ccgtctagtt ccaaattcca atagtggatc ttctccgatg acagatttga tacgagctgc
    42901 ttttgtcgcg atcaacgttt ggaaattgac catattcaac agtgctgttt ctactaattg
    42961 gcagtgcgct aaaggccctt ccacttggat caatggttca tttgcaaaaa ctagttctcc
    43021 ttcacgagca gaacgaactg ttgctttaaa ttcaaaattt tgtaaatact ccaaaaaacc
    43081 ttcaggatag acttctaatc cacgcaagta agcaatatcg gaatcattga acgtcagatt
    43141 ctctaaataa ttgactaacc gttccaatcc tgcaaaaacc gcgtatccgt ggttaaaagg
    43201 catttctcga aagtaacatt caaaaacagc atgtaaatca gctcgaccta attcccagta
    43261 tgtctgcatc atattgattt gatatagatc cgtatgcagc gtcaaactgt cgtctgggta
    43321 agtattattc atcttgtatt actcctcttg gcaagttttc atttcctacc cattataaca
    43381 aaaaaaatta ataggaaaag aaatcttcca ttttttctga aaaagcacat gttttacgga
    43441 tatttcttgc taaaaatgaa ctccatctgc taaaactatc ctttcttatt ggtatttaac
    43501 ctaaacgaga aagaatactt attttattca atgtatccga gacacgaaga aattagcgtt
    43561 ttctattttt cttacttgat aaaataagta gattttccta ctacaatcgt agccgttaag
    43621 ataccgatga ttgaacaaat ataaccaaat gtctctacaa cataatcaat ggcaaaagtt
    43681 tttgaagcaa taatacgtct ttcctttttt gcttcttatc cccaaagaaa cggatataac
    43741 ttattatgta ttgaattttt ttggaaaggc gaattatgac aagatatacc ttgcgttgaa
    43801 aaaactcgcg gaaaaaatat cacacgtttt taaaaagata caaagtaaga aagcagctgt
    43861 caatagaatg gaaacaaaaa tggcgagaca tcaatcagtc tcgccttgtt ttttattgta
    43921 tacattagat atggagcagg ttaccataaa gatgattatt agtcgttatt aaatagaatc
    43981 cttcgtattt ttttgttcta tcactacaat atctttattt ggacgtttga attgtattcc
    44041 ggcacgttct agctcctgtt tcactcctac atggactttg acatcttctc gtgtcatttt
    44101 taaaacgtga tgaatcgcat agtcgatttc ttgccttgtg atttttttct tcgtattgat
    44161 catcacgact tgctttttat catatgtgtc taccaatagc agttgtgttc gatctaacga
    44221 aaataatttg taataattaa tagccgtatt cccaaaaaag aattccacta ctttaggata
//
LOCUS       DAYYKN010000010        38220 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000010 DAYYKN010000000
VERSION     DAYYKN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.010"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..729)
                     /locus_tag="ACHBB7_000440"
     CDS             complement(64..729)
                     /locus_tag="ACHBB7_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM9208421.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(876..1481)
                     /gene="dhaL"
                     /locus_tag="ACHBB7_000441"
     CDS             complement(876..1481)
                     /gene="dhaL"
                     /locus_tag="ACHBB7_000441"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM9208422.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(1509..2519)
                     /gene="dhaK"
                     /locus_tag="ACHBB7_000442"
     CDS             complement(1509..2519)
                     /gene="dhaK"
                     /locus_tag="ACHBB7_000442"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM9208423.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(2494..2889)
                     /gene="dhaM"
                     /locus_tag="ACHBB7_000443"
     CDS             complement(2494..2889)
                     /gene="dhaM"
                     /locus_tag="ACHBB7_000443"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM9208424.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            3043..3363
                     /locus_tag="ACHBB7_000444"
     CDS             3043..3363
                     /locus_tag="ACHBB7_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM9208425.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(3475..4332)
                     /locus_tag="ACHBB7_000445"
     CDS             complement(3475..4332)
                     /locus_tag="ACHBB7_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9208426.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(4440..5744)
                     /locus_tag="ACHBB7_000446"
     CDS             complement(4440..5744)
                     /locus_tag="ACHBB7_000446"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9208427.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(5741..6250)
                     /locus_tag="ACHBB7_000447"
     CDS             complement(5741..6250)
                     /locus_tag="ACHBB7_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208428.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(6425..8560)
                     /locus_tag="ACHBB7_000448"
     CDS             complement(6425..8560)
                     /locus_tag="ACHBB7_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM9208429.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(8643..10298)
                     /locus_tag="ACHBB7_000449"
     CDS             complement(8643..10298)
                     /locus_tag="ACHBB7_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9208430.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(10300..10614)
                     /locus_tag="ACHBB7_000450"
     CDS             complement(10300..10614)
                     /locus_tag="ACHBB7_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9208431.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            10784..11044
                     /locus_tag="ACHBB7_000451"
     CDS             10784..11044
                     /locus_tag="ACHBB7_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM9208432.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            11058..11372
                     /locus_tag="ACHBB7_000452"
     CDS             11058..11372
                     /locus_tag="ACHBB7_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM9208433.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(12102..12656)
                     /locus_tag="ACHBB7_000453"
     CDS             complement(12102..12656)
                     /locus_tag="ACHBB7_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM9208434.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(12782..14711)
                     /locus_tag="ACHBB7_000454"
                     /pseudo
     CDS             complement(12782..14711)
                     /locus_tag="ACHBB7_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(15017..15733)
                     /locus_tag="ACHBB7_000455"
     CDS             complement(15017..15733)
                     /locus_tag="ACHBB7_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9208435.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(15734..17557)
                     /gene="glpO"
                     /locus_tag="ACHBB7_000456"
     CDS             complement(15734..17557)
                     /gene="glpO"
                     /locus_tag="ACHBB7_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM9208436.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(17571..19067)
                     /gene="glpK"
                     /locus_tag="ACHBB7_000457"
     CDS             complement(17571..19067)
                     /gene="glpK"
                     /locus_tag="ACHBB7_000457"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM9208437.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(19203..20663)
                     /locus_tag="ACHBB7_000458"
     CDS             complement(19203..20663)
                     /locus_tag="ACHBB7_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9208438.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(20653..21948)
                     /locus_tag="ACHBB7_000459"
     CDS             complement(20653..21948)
                     /locus_tag="ACHBB7_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9208439.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(22280..22957)
                     /locus_tag="ACHBB7_000460"
     CDS             complement(22280..22957)
                     /locus_tag="ACHBB7_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM9208440.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22987..24078)
                     /locus_tag="ACHBB7_000461"
     CDS             complement(22987..24078)
                     /locus_tag="ACHBB7_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM9208441.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(24179..24613)
                     /locus_tag="ACHBB7_000462"
     CDS             complement(24179..24613)
                     /locus_tag="ACHBB7_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9208442.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(25197..26654)
                     /locus_tag="ACHBB7_000463"
     CDS             complement(25197..26654)
                     /locus_tag="ACHBB7_000463"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9208443.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(26651..28546)
                     /locus_tag="ACHBB7_000464"
     CDS             complement(26651..28546)
                     /locus_tag="ACHBB7_000464"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9208444.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(28604..30379)
                     /locus_tag="ACHBB7_000465"
     CDS             complement(28604..30379)
                     /locus_tag="ACHBB7_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM9208445.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(30429..31925)
                     /locus_tag="ACHBB7_000466"
     CDS             complement(30429..31925)
                     /locus_tag="ACHBB7_000466"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9208446.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(32119..33741)
                     /locus_tag="ACHBB7_000467"
     CDS             complement(32119..33741)
                     /locus_tag="ACHBB7_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9208447.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(34069..35382)
                     /locus_tag="ACHBB7_000468"
     CDS             complement(34069..35382)
                     /locus_tag="ACHBB7_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM9208448.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(35627..35851)
                     /locus_tag="ACHBB7_000469"
     CDS             complement(35627..35851)
                     /locus_tag="ACHBB7_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208449.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35871..36293)
                     /locus_tag="ACHBB7_000470"
     CDS             complement(35871..36293)
                     /locus_tag="ACHBB7_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9208450.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(36435..37145)
                     /locus_tag="ACHBB7_000471"
     CDS             complement(36435..37145)
                     /locus_tag="ACHBB7_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM9208451.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(37163..37660)
                     /locus_tag="ACHBB7_000472"
     CDS             complement(37163..37660)
                     /locus_tag="ACHBB7_000472"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9208452.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(37697..38149)
                     /locus_tag="ACHBB7_000473"
     CDS             complement(37697..38149)
                     /locus_tag="ACHBB7_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9208453.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
BASE COUNT        10740 a         8217 c         6451 g        12812 t
ORIGIN      
        1 aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt
       61 tgtctattga aatttttccg cacctagctt ttgatatagt ggtgctagtt cttgtaaggt
      121 ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga tagaagaata gtttacccat
      181 ttgaaaggtt cggtctctca tcgctttttt caactcttct tgtttctttg tatcggacaa
      241 atagtcagac aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata
      301 atccttgata tcgactgttt tgctccactc tttcagtatc gcaagcaact ggttatacgc
      361 tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg tacatcaaat agatttggta
      421 ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag attcgcttcg
      481 gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc gtccagcttt cgattttagg
      541 tttgctcatg ccgtacatat caggcaaaag tgcagctgtc tggagctgaa tctctttcca
      601 tttttgccag tgttctttat attctttctt gatttgttca atcttatctg ttggatggct
      661 ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac aactgataat ctgattttcc
      721 aagttccatt gtttcgcctc tttccgttat cttttgtatt ctacctgaat tttgcgatat
      781 ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt cataagaaca gccaaacaaa
      841 aaactgcgat gagcatcacc gcagttttat tgaacttaag gttgattgat cacatctaaa
      901 agcgttgaaa ataaaatggc actagaagca gctcctgggt cgatatgtcc gattgcacgc
      961 tctcctagat aggaagctcg tcctttttta gctaccaaga ctttggtgtg ttcttttgcc
     1021 tggtcaatga tttcttttgt taaattccct gccttcaatg cctctgtgac tggcagccaa
     1081 acatctaaca tcgttttatc gtcagcttct gcctttcctc ggctttgaat acccgctgtt
     1141 ccctgctcga tcacttcgcc taatttttct tgagaatcaa tagaatcgac ctcttttgtc
     1201 gctttcatca tgttcatgaa tgcagatccg tataacggac cagaagcccc accgactttc
     1261 gatatcatcg ccatagagaa tactttgaat gtatcgctga ttgtttcagg atgatcggct
     1321 tgaaaagctt cttcataagc tgaaacccct cttcccatgt tatttccatg gtctccatcg
     1381 ccaatcgggg tatctagatc actgagatat tgtttgttct gattgattgc ctctgcaaat
     1441 tgcgtgagcc attcttggat ttcttgaacg gttagtttca tattatcacc cttttatctt
     1501 gagatttttt accatgaaat cgtctgtaca ttgctttcta aagaatcttt ccattgtgtc
     1561 tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat ctagtgaggt cataaaatta
     1621 ccgacttttc ggaaaactac attgacgcct ctttcttcca acagagctaa aacatcattc
     1681 atgaacacga actgttccat caaaggcgtc ccgcccattc cgttgactag tacacctgct
     1741 tcttttggta aattcgagta atcatctaaa atcttagtaa caagttcctt tgccagtact
     1801 ttagaaggct gcatcttttc tcgacgatag cctggctcac cgtggatacc aacaccatac
     1861 tcgatctcat cttctcctag ttcgaatcct ggtttcccta cttctggtac agttgctgct
     1921 cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct tttctcccag ttctttcaac
     1981 tcagctaggg aagcaccgtt tcttgctgca tcgcccacga ttttgtgtac aaggacagta
     2041 cctgcaactc cgcgttttcc agcagtatac gtactatttt caacggcgat atcatcatct
     2101 acgatcacac tttctacttc aatatcgtcc atagcagcta attcctttgc catatcgaag
     2161 ttcaatgcat cccctgtata attcttaacg atcagcaaaa ctccttttcc actatcggct
     2221 tccttaatag ccgtctgaat ctgatctgga gttggagaag taaacacatc accaagcacc
     2281 gctgcactta acatcccttc ccctacaaac ccggcatggg atggttcatg tccgcttcct
     2341 ccaccagaaa ccaaaccaac ttgttccgct ttctgattct tggctacaac acgggaagat
     2401 tctacacgat gaacaagttc tggatagctt tttactaatc cttccagcat ttcttcgaca
     2461 atattgcttg gatcattcat gatttttttc aatttattcc catcctttct ttccggcatt
     2521 ttgtgcttct gttaagattt gctgagtatc atctgttata ccggcagtga tggctgctgc
     2581 aaaagctcct tccactaaag gcgcatccac cagatgataa cgttgctgct gctcttcttc
     2641 caacatgtct tttgccagtt ccgagttcaa taccgcgcta ccgatatccg caaaaacaaa
     2701 ataactagct gcatcgcttg attcattgac tgcttctacg attttcatcg ggtctgatcc
     2761 gatctcgcca tttattccgc cgagagaaaa aatttgcacc tcttcggatt tttgcatctg
     2821 ctcaatcatt tctttaatac catctgttac tttttgacta tgagaaataa gcaaaatact
     2881 ttttttcaca agaatcttcc tttcttttac atagttgcta acgattggtt tcgctttcat
     2941 taatgaacaa ttcaagtata taataaattt ctaattttgg gaaaatatgt gtagtgcttc
     3001 ttgaagtttg ttatacttta attaaaagag aggagtgatc gaatgattac aacaacaacg
     3061 aacagtgtag agggaaaagc cgtagtagct tataaaggga tcgtttttgg tgaggtcatt
     3121 actgggatcc atgcatttaa agacttggag gcggggttgc gaaatatctt tggcggccgc
     3181 tcaaaaagct atgaaaacga actaatgggt gcaagagaag aagcacttgc cgaaatggcc
     3241 gaacgtgcaa aagcattagg agctgatgcg atcattggcg taaaaatgga ctatgaagta
     3301 ttaggcagcg acaatggcat gctgatggtc acatgtagtg gaacagctgt ctccacgaga
     3361 taagtagggg acatctgttt taataactaa agagagatgg gacatgtagt ggcactcaag
     3421 aattagattt ttcagtctaa cttcgaggta ctctaccttc ccatcctctc ttttttattt
     3481 ctttttgcgg atgattccgt aagctgctgc gactaacaca cccgcccctg ctaaggaata
     3541 actgacagaa gctgtttttt catttgtttt tggcaaagtc tgacttgttg atttggatgg
     3601 ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag gtcactgatt tttctcctgc
     3661 cgaatgcaaa atatctttta ttcgttggaa actagctgct aagtcagctt tttttgcttc
     3721 aaaatcatta gcaactgttt gtgcttcgct gatctttccg tggattgctt gactggccgc
     3781 tgtgatatcc gcttgagttt gacttagctt tccagaagct gtttgagcaa cctgatttaa
     3841 ttgctgattg aattcttttg cttgttggat cttcccgtca agatctgttt ggacagcttc
     3901 cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt gcacggtcat ttcccacttt
     3961 atttttaaga tcagctaaat cttgatcgat tgctgcttgg tcttctttca atttgttttt
     4021 tgcagcttct aatgcttctt cgagttcttt tcttttattt tccaagtctt gcaaatgatc
     4081 gatttggtca gaagaggtag aactatttat tgatgtatct tctgaagatg aaatcgtttc
     4141 tcctgaatta tctgtcggta cattggaagt catttctgtt tccgaggcac cgttttcagc
     4201 accagaagag cttatggttg ttgaaggttc tgtttgagca ggttcagaag tttccgcaaa
     4261 agctgtctgt cccaataaga aaatactgga aacagcaatt ggccagatta acggcttcat
     4321 tctttttttc atcacttttc ccatccttac tactttttaa ctaatttctt attataagca
     4381 aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt ctaaaaaacg gtgcatcttt
     4441 tatttttttg tacgttccgg gcttttccat gtcccgcttc cataacctaa aatcgttttg
     4501 actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca ctatccagaa caaaagcata
     4561 tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc gatcatctac aatcagcgaa
     4621 gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga tgaacacaaa actgaatgta
     4681 aaagtaatat aaatctgttc gtagtttcct tgatatacat aatgcccgat taatccagca
     4741 aaaagaacta cggaaataca aaagaatatt gaccagacaa tactcaatgt ttgatcaatg
     4801 aagatgatcg ttcttccaat atgtttaaaa ggatggagca tcactttttt gaaattcgtc
     4861 agccagactt ctgttccacc ttttgcccaa cgttttctgt gtcgatatag catcttcaac
     4921 gtcacaggta cttccatgaa aaagataatc tcaggagcaa aaacagataa ccagtcattt
     4981 aactgatgat cccaagcaat actgatgtct tctgtcgctc gatcttgtct gaatccgccc
     5041 acatcgatca aggcttcttt tcgatacatc gtatttgctc cactgtacgc ataaagacca
     5101 ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga tacttgagaa ttcaaccgtt
     5161 tgagattttg caatcaattt ggtacgattt tggacatcca tatttgcagt gaccgcagca
     5221 atatgtcttg caccagggcg aatgaaataa ttcacatacc taatcagtgc atctggctcc
     5281 ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt ttgcaaaggc aagtccgata
     5341 ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa ccacacgaag attagcgtat
     5401 tttttcatca gtctagctaa tatttctggt gtttggtccg ttgaaccatc gtctgtcact
     5461 aatacctcat agttgtgata attcaatttc gtcatcaaat actcgattgt atattcgatc
     5521 acaatttctt cattatgggc tggcaccatg atcgtgatga acggttcgac actcaaggga
     5581 acatctaccc attctttttg cttatgtttg tataaaaaca cataacacca gacaccaata
     5641 aaccatacaa atccgcctaa aatcggataa gaaacgagaa aaaaatacat aatagacaaa
     5701 caagtatcta agaataaatt acctgtatgg gtgattgaga tcatttcagt tccaccccat
     5761 tttctttata caaatctgca atgaatgtcg tatctagatt tttctcttcg gagacagaat
     5821 aaaaacggac cgtttctctc tcttcttttg ttccaaaacg ttccgtatac acttcttcaa
     5881 gtaattcttg tctttttttc aatttttcat cttcatattc aaaagattta tttacttttt
     5941 tgtatagatt tcgattgttc cacctagtca atacaaatga ggtaatcagg aaactgaaaa
     6001 aaatcaatac aaaaaagccg attaagaaat acagcatttt cttttctgat aaaaaagcgt
     6061 acaaggaaaa atccttggct ctttctggtc tcgttaatgg gaaaaagacc catataaaag
     6121 gaagaacaac gatcatccat cctaaaagag ccactaacgt ttgccaaatc tttaaccagt
     6181 aattaccttt tgaaaaatac ttatctttca ccaaaattga tgcaacttct tctcttgtta
     6241 cttttttcat atcctcacct cacgtttttt tgttacgacg taggagttta ccatacattt
     6301 catttctttt cacataaagt gttcctaaat ttttcctaag aaaaagaaca aaacatttct
     6361 catcagcaaa tgaaaaatat tttgttcttt ttatcagttg ttctttttat tttttatctc
     6421 ttaattatgt tgctctttcc aatacgacaa tgcttttcat tcctctagat atcgccgtgt
     6481 acatttgtct gcgtcttctt tgctcatctc catcgttttc aaatggatat aagattacat
     6541 gatcaaattc cagtcctttt gctagactca aaggaatgat ttggcagttt tctaatccat
     6601 atgaattttt tttaatcgat tcttgtagcg tttgggtttc ctcttctgtt tgggtgataa
     6661 ctgccgcttt ttctcctttt agagaaacca aaatctcagc tagtattttc aacaattctt
     6721 cagaggatcg aaaagagcgg tattcgggtt cttttccttg cggtcggatc gatacgatgt
     6781 cgatcttctc ttgatccaca gcataggttt tgaaaagctc tgtgattgct ccgctagaac
     6841 ggtaactatt tctaagatca taccgatgga tcggcagatg gtagtcgtcg aaacaacgca
     6901 taatatcagc aaaagtagtg gaagaattaa agattgcctg attctcgtca cctactaaag
     6961 taaagtgagt ttttggaaat aattcgatca atagacctaa ttgtgccagg gtataatctt
     7021 gtacttcatc aatcaataga tagcgaaatg ctggtacatt taccttttca acaagtaaat
     7081 gacgaatagc taatataatg actgcttcat caacagtacg tgtgggttgc caagcataag
     7141 ctgagccata tcgtttttca taaattcttt caaacaactg atgctcatcc acccatgcca
     7201 tttcttcgat ttgtctggtt atttttcgat attttttttg taaaagtttc cttgcataag
     7261 ctacaatact ttgttccgaa tcatcagaaa tcaatttacc gaataattcc aactgcctat
     7321 cttctgtcaa cgagagtacc tgattctgca cagcaggact ttttgcttgt ttcaaaagat
     7381 gattcttcca ttcgctcatc agtgcctttt tcgttgcttg aagcctatcg atcaatttag
     7441 gatgagcagg aactttctca tatatttttt cgatcgtttt tttagaaagg actgtttttc
     7501 catttttagt aagtccacgg ataaaattcg gatgatctaa aaacaaagga tctgattgtt
     7561 ttagattatc gataaacttt ttgctacgca aacgttctgt ctgctccgat acattttctc
     7621 ccgtgatacg tttgaaataa gcctcttcac cttctatttc ctctgctgaa agctgtgaaa
     7681 ccagctgtat gatggttaga ttcagtggat ttctttcacc taatgatggt aggacattcg
     7741 aaatatagtc gataaaccga tgattaggtg atagaatcag caagtcatcg gatgtcatcg
     7801 tatctcgata taaatagagc agatacgcaa tacgctgcat gatagcagag gttttaccac
     7861 ttcctgctac gccatttacc aagatatttt gacttttcac atcccgaatg attgtgtttt
     7921 gttccgattg gatcgtagca gtgatagcct tcatttcatt ggtctcatct tggcctaaga
     7981 cttctaataa tacgtcatct tgaatagcta cagacgtatc aaaaaacttg accaaacgat
     8041 ctctttccaa aagaaattgt cgtctttctt taataaccgt atcgatttta ttttgacgaa
     8101 ccgtataaga agatggacca atcatgttat tgtaaaaaag ttcggcaatt ggtgaacgcc
     8161 aatcataaac aagattttca tctgcatcat ctgtgaaatt tgctgtaccg atataaaagg
     8221 attcttttgg ctcattatcc agaaagtcta caacgacttt tccaaagtag ggacttttca
     8281 ataaacgttc gatctttgct agctgatttt ctgcgttttt ctgtttgata ttcatctgat
     8341 cgatttctct atttttcatt tcaatcatcg aaaaagtatc caggttatct aagtaactgt
     8401 caaaattcaa tcgaagctcg ccagccattt ctttcaacga ctggtttcct tctatttttg
     8461 ccgtatccat cgattcccgc agttcgtttt ttttatcaat tagcttttga tacacgttct
     8521 tcagatatgc ttcttctaac tgtttttgtg tgttgaccat acacattctc ctcttttttt
     8581 aaagtggtgg aaccgattag tttacctctt ttaagagtcg aatgcaagct ttttaacgag
     8641 tatcatttgt taggatatat cagttcttca ggtcggacag tttgatataa agaaaatgca
     8701 ccatcaaggt tttttacgtg gtagccagac tgcttcaaga ttctttccgc tagatagcta
     8761 cgtaatccgc tatgacagct gacgatatat tcttgttggc tgtctaattc atttaatcgg
     8821 tcccgaagtt cattcaaagg gatatgtttg gcttccttga atttgccctt ttccaattct
     8881 tctgctgttc gcacatctaa taaaattttt ccttttgcta gttctgtcgg caattcatgc
     8941 cattgtacgg tttcactaag cccttctgca atgttcattg ctgcatatcc tagcatgttt
     9001 actggatctt tggctgagcc aaatggcggt gcatacgtca attctaattc cggtaaatcg
     9061 aagatcgtca aatgcccttt gattgctgtt gctaaaatat cgatccgttt atccacacct
     9121 tttgccccaa caccttgtgc accataaatc tcgccagtct ctggatggaa gatcaacttc
     9181 agtaaaatat ctgttgcttc tggataatag cttgcatgat cttttcctga tacatgaatg
     9241 acttcaaaag acagttcgtt ttctttggct gttctttcac ttaaaccggt ggatgcggca
     9301 gtcattccaa acgttcgtat aattgctgtt ccaataccgc cttgatttct tcgagaaatt
     9361 ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg ctggtgaagc aagagaaata
     9421 agtgcatctt ttccagtgat ttgctgtttt acaacgatag catcccccac cgcataaata
     9481 tcaggaatat tcgtttgata acgttcgtct actaggatac ccccgcgtat gcctaattcg
     9541 attccagcgg tcactgctag ctggttatca ggacgaacac caatggataa aataactaaa
     9601 tcagcaggga tggtacgccc atcatccagt cgcaatcgtt tcccgtggtc ttcaaatcca
     9661 gcgacagcat gagaagtaat cacttctact ccttgatgag acaattcttt ttctataaaa
     9721 gctgccattt cttcatccag tgttggaaga atatgtggtg cttgttcgat caccatgact
     9781 tctaaacctc ttctttttag gttttctgcc atttccaatc cgatgaatcc tgcaccaacg
     9841 atcacggcac gctttggctg tttttctaat gcatgcatca ctttatcgat atctggtaca
     9901 tttctaatag aaaaaacatt gtcggcttct gttatccctg gaatcgatgg aacaactggt
     9961 tttgcaccag gagataaaac caatgcatcg tactgttcaa tgtgtttttg accctcatgt
    10021 acgacttcta ccgttttgtt ttcgggaaag atggctgtca cttcatgatt tggacgcaca
    10081 tctaaacaaa aacgagcgga taatgattct ggcgtttgga caagaagatt ttcccgttca
    10141 ctgatttctc ccgaaagata ataaggcaag ccgcaatttg caaaagaaac atacggtcct
    10201 ttttcaaaaa caacgatttc tgcatcttcc attaatcggc gaagccgtgt agcagcagac
    10261 attccacctg ctacacctcc gacaataacg attttcatct tatttccctc ctactgttcg
    10321 atcaaaccat tgattcatgc caccacgtac attcactaca tcataaccag atttctttaa
    10381 ttctttagct gcttgtttac tcctcatccc tgattgacaa atcacataga cggtcttttc
    10441 tttgtctcca tcatatctgt ttatactttg taatggaaca tttttagctc cttttatatg
    10501 cccgccccgg tattctgcag gtgttctaac atcaagtaac gctggatttt ccaaaagctt
    10561 ttcctttaat tctgggactg ttatcgattt tattgattga aatagcaaat tcattttctg
    10621 cacctcattt acatttttat aaatacctca tggggtatat taaacaggag ctttattaat
    10681 atgtcaacca gaatgactct tcattttttt cactactttt tatatttgca taaaataccg
    10741 ataaggggta ttatctaatt ataaaaatag aggagaatgt gttatggaga ttgataataa
    10801 aaatatcctg aaccgtttga aacgtacaga gggacaaatt cgtggtatcc aaaaaatgat
    10861 cgaagacgaa aaagaatgta tggatgtcat cactcaatta agcgctgttc gttctagtat
    10921 tgatcgcgta atgggcatga tcgttgcaga taatctgaaa aattgctttg agaaacctga
    10981 tagtaatcca gaagaacaag cggctaaact agaacaagcg atcaagatga ttattaaaaa
    11041 gtaaaggaat gatttcaatg aaagtcgttt tccatattga tgaattagaa aaatggtcag
    11101 aaacagggaa aaacgtaaaa aacctgataa aagcttctcc agaaacgacg attgtcgtat
    11161 ctgtaaatgg catcgcgatt accggttatc tagactctgc caatgcagaa ttcttagatt
    11221 tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg tgctaatcat atttcagaaa
    11281 gttctttacc tgaacaagta atcgttgtac cagcaggtgt ccttgattta gttgaactgc
    11341 aatcccaagg ttatgcttat atcaagcctt agtcagtaga ggttgcgaca gaagtgatca
    11401 gcttcaagaa atttcggctt atttttcgaa gaagctactt ctggaaaacc gtgaatcaaa
    11461 ggttgtgaaa acgctgtcct gcatcaagta ataagaacag ccaaacaaaa atagcttctc
    11521 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttaagg
    11581 aagaggttgt gaaatcagcg gtttgaccta agtattagag aaaaaaacgg gaaaatagct
    11641 cctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa caccgtttaa
    11701 ggaagagatt gtgaaaaagt cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg
    11761 atttctacat ttttgcataa atttggctta ataccgagaa actggctttt gaataccgtt
    11821 taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa
    11881 tagctgctca tattttgccg ttttttgagc taataccgaa ggtcagctga ttgaacaccg
    11941 tttaccaaga ggttgtgagc ctaccattta actccgaatc acaagaaata atttcaaaaa
    12001 caacttctcc tgtttttggt tcggttggct tattatcgag aagtagctac tgggagtcta
    12061 tttatcaaaa aaaggaatgc gacataggtc acaccccttt tttattttct ttgaaacatc
    12121 tttcggtact cgacagcata caagacaaaa gattgtaaaa gaccgtaagt gatgacggca
    12181 aaaatgactc tcacccacag cgttggtaat tctgggaaaa gcagcaaaag gatcatgcca
    12241 acatagatga cagcagtcgc tactttgcca taccacttcg ctccacttaa tttgatattt
    12301 ttagatagcc cgattaatcc agctacaaac ataaagcctt cttttatcaa gaatagacca
    12361 aacaacagcc aaagccacgg tcgataccat gccatagaca aaatcagaaa aagttgtgtc
    12421 aatttatctg caaacggatc aagcaccttc cctaagtctg ttacctgatt atatctccga
    12481 gcaatgaccc cgtctagata atcagacaaa cctgagacaa caatgaccgc tcctgctata
    12541 taaaatgact gtctcagcac aaaataaaga taaatcggca gcaaaaaaat acggaatatc
    12601 gataaaagat tcggaatcgt ttgtatttct ttacgccagt ctgtgcttaa tttcatttta
    12661 tcgccctacc tcctcagcac tcttttcttt ttattttagc ttacacgggg aataaaacca
    12721 atgatttcac tgtttttatt tttctttaca gacactgata ttatgagcta atttgagata
    12781 atcacttgct gttttcatcg tttactcttc tttcttcctg tcgtttgcgc atttctttta
    12841 ctagtacttt atgagctttt tttgatcctc gctctaggtc acgctgccgt ttacgtgttt
    12901 tataatcgat aaacagactg tctaaatcgt aacctgtcgg gtacagatct tctgctgaac
    12961 gcaccagacg aacacgctga cgaggcacgt ggattatttt tttgtctaaa tatacatcaa
    13021 tcgtatcttc acctatatct tcataaacta acgcttcttt ttggtattca ttgacaaata
    13081 tccgatcacc ttttgcaaag agctgacgtt cttttttatt ctccttccta tttgtaactg
    13141 ctgtcttagg aagaaattct tttttctgtg tccgataggc tttttccgcc aagtaacgct
    13201 ctgcttgatg gatcaatttc atcgacatat ccattttatg agcaatccac aaagcttggc
    13261 tttctcctgt ttcttccaac ccgcaactga tatttaggac gaagtgcttc ccgatcgaaa
    13321 gccatagcag ccggcacaaa atcttcatga tctccggcga atttcttgat ttctccataa
    13381 tgggtcgtgg caatgatcaa tgccccttgc tcatacatag attccatgat tgcgatcgct
    13441 agtgcagccc cttcatttgg ctctgttcca ctgccaatct catctaaaag taccaacgta
    13501 ttccttttta tctttttcaa aatagcagcg atgttttgca tatgaccaga aaatgtactc
    13561 aacgcatttt ctaaattttg gtggtcaccg acatcgacga aaatctcatc aaagacagca
    13621 agctctgtcc cttcttttgc cggaacttgc ataccgaaca tcgccatcaa agtcaataaa
    13681 ccgactgtct tcaacacgac tgttttgcct ccggcatttg ctccggtgat aatcaagcct
    13741 cgatagtctt taccgatttc aacatccaat ggtaccgcat gctcaggtaa aaatggatgt
    13801 ctgccttgct tgataatgat atgttccgat ttattgattc taggtgtgct tccattgatt
    13861 tctcggctaa attttgcccg agcaaagatg atatctaaca ccgtgatcgt ttcgatcaat
    13921 aaatcgatca cttcttcgtt ttctgcgata gcaccagtca atgccgccag gacttgatat
    13981 tcttcactga tttcttcagc ttttaacaac tgatagtgtt cattcaattt agaaatcaca
    14041 gttggttcaa taaaaaccgt cgttctttta tttgattcat cgataatcgt tccatctatt
    14101 ttatttttat agctggcttt gataggaatc gtatagtatt cgcctttttg aacaatcatt
    14161 gcttcttgga tcatttcttt gtttttcgga tgacgcaaga atttcagtaa ttttgactgg
    14221 atttcttttt cgattagttg taattgtttg cgtactttac gcaaattacg tgacgcgtcg
    14281 tcactgactt tattattttt tattttctgg tcaatcaact cttctacatt tatcaaatca
    14341 ggaagatgtt tgctataagc aaacaacaag ggtgtctggt attgattctt atcaaaaaac
    14401 tttgtgatca tccgactgct tctaagaaaa tccgcatatt cgatcaaatc ggttggctgc
    14461 agaatcaacc cttttttgac ttgttctgtc agtgcatcga tcctcggcaa ccccataaat
    14521 ggaacatgct gactgctttc taagatcaag cgtgcttctc ttgtttcttc ttgacgatcc
    14581 aagacagttt gtaggtttgt cgagaccgtc atatcgctga ttcgtttttt gctatagttt
    14641 ccaattgctc ttgcttggat ttcctttttg atccgatcaa attgtaattg actaatcatt
    14701 tcgttgtcca tattctctcc ttttcttgaa gagatacctg tcccaaaata aataagggcc
    14761 tagagataaa taaaaggata gaacacagct taaaaaagtc atgtcctatc cttattagaa
    14821 tacaaagata ttgttttcct gacaggtacc gtttcataag cacaccaaat aaactttgtt
    14881 tattggctat tcgacttact gattagatac cagctacact cacaaaacat accccattct
    14941 tctaggtctt atttattttc cacttaacta tagcaagaca cattttggtt gtcaaacttt
    15001 agtatgtttt atagatttac accattaatg aatagacacc taccgcgata attgctccga
    15061 tcattggacc tgcgattggt acccatgaat atgcccaatc tgaagttcct ttattagcta
    15121 ttggcaatac cgcatgtgct aatcgaggac caaagtcccg agcagggttg attgcatatc
    15181 ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa tccacctaca gcgaatacgt
    15241 tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa cccaagaact aatacaaatg
    15301 taccaattaa ttctgtgatc acatttgctg gataattccg tatagcagga cctgttgcaa
    15361 acgttcctaa aatcgcgctt tcatctttcg ttgcattcca atgtggcaaa taagaaagcc
    15421 aaacaagaat ggcccctgca aaagctccta aaacttgcgc aatgatgaaa ggaacgacaa
    15481 gattccaact aattgcccct gtcatcgcca tagctagaga gactgcagga ttcaaatgag
    15541 ctgggcccat gaatccagaa atatagacag ccattgttac agcgagtccc caaccaaatg
    15601 cgatgacaat ccagccagaa gcctgtgctt tacttttgtt taaattcaca gccgcacaca
    15661 caccatctcc aagcaaaact aggatcagtg tcccaataaa ctcccccatg atttgagtca
    15721 tatcactgtt catttatttc tcccccttta gttcccgcag atctgattct gcaatcaatg
    15781 cttctaaacg tttagtctgt tgcgccttct cctcttctgt ccattcaaaa tatccagcaa
    15841 ttgcatcgat cacaggttgc ttgatctcat caagctgatc tcgttcaaac aacagatgat
    15901 ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat ttcttcttct aaaccataac
    15961 gtaaccgagc tgactcagcg agactcaaac caggataagg tgccatttct tttgctaatt
    16021 caaagatacg tcgggcatta gtcccgtaaa aatcagcgat ataagtagca tcttcttcct
    16081 ctaaaccggc tgctactcct tcttttgcta attctgtcac tgtttcttct aatttagttg
    16141 gatcgaagtt tccacctgaa atctgatatt ttttagagtc gatttctttc gtctctattc
    16201 cgtattcttc ttttaataat tggcgtatca atcgcaaagc tccttctgcc atcttacggt
    16261 aatcggtgat tttcccacca gataaagtca gcaaaccatc tggttctctt tctaaagaac
    16321 tacctctgga aatcgtagaa ggtgcttttt catcacggct gttttccaaa tggttcaaca
    16381 cgtcttctac ttcagcacga gaaactttgt tttccttata ttcacttact gtatcgacaa
    16441 ctttattgaa gctcttgtct gaaatcgatc cattatctcc accattgtaa tcagaaccag
    16501 aattaccaat caacagtgga cgaagtcctg cccagcttgc ttcgatatca ttcaatgtga
    16561 tatttgcttc tggatagcga tgattgatca catccaatag ataatctaca tcttcttgtg
    16621 tgactttcgg atccgtaaaa tctccttggt aatccgtatc tgtcgtacca aagtaagtct
    16681 tgttttctct tggaatagca aaaaccatcc gcccatcttg ctttcctgta tcgaagtatg
    16741 tgggttgcgg tacaggcagt tttttcgcat ctacaactaa atggatccct ttagttggac
    16801 gcattttagg agagactgga cgcgtaaaat tcaagtttcg tactttatct acccaaggac
    16861 cactcgtatt gatcactaat tttgctttga tttcgatcac ttcatctgtc agcaggtcac
    16921 gagctttgac gccaacgatt tgatcgccat catataaaaa gccaaccgct ttcattttac
    16981 ttactagata agccccatct tcggcagcct ttttgatatt atcaatcact aaacgggcat
    17041 cattgttgcg gaaatctaga tagacacccg cgccttttag cccttctttt ttcaaaaacg
    17101 gttctctttc caatacttct tctggtgtca gcgtataatt ctcatatttg gttcctgtca
    17161 catttgccag tttgtcataa aggtccattg ctactttgac cgagaacata ttgaaggttg
    17221 ttgctccttc gccctcatag attggcaaaa gcattggatc tggttttggg atgtgtgggg
    17281 caatactttg tacgactgca cgttcaccaa ctgtgtcagc tactacttcc acatcaaatg
    17341 tcttcagata acgaataccg ccatgaacaa gtttggtcga gcgagaggaa gtcccttctg
    17401 caaaatcctg catttcgatc aatcctgttt tgattcctga tgctgctgac tggactgcca
    17461 caccggcacc agtgattccg ccaccgatga tcaacagatc caattcttct tgctgcattt
    17521 tttgaatgct atcttgtctt gtcttgtttg aaaacatatg gatttcctcc ttattcaaat
    17581 gcttgggtag cttttactgc ttttttccaa ccgttgtaaa gtttttctcg gcgctcttcc
    17641 tccatttgaa cttcaaatag cttgccagct tcataaaatt cgcggatctc gtctgtatct
    17701 ttccaataac cgacagctaa tcctgctaaa aatgctgccc ctagcgccgt tgtttccaag
    17761 ttttctgctc gttggatcgg tacattcaag atatctgttt ggaattgcat cagatattca
    17821 ttgtttgctg caccgccatc tactttcaat acaggaatct tgattccagt gtcatcttgc
    17881 atcgtatcga tgatatctct cacttgataa gcaatcgact gtaaagtagc tttaatgata
    17941 tcttcttttg tcgtaccacg tgtcaagcca aacatagccc ctcgtgctgc ctgatcccaa
    18001 taaggagcac ctagcccaac gaatgcagga acaacataaa cctcatcttc gcttgtagag
    18061 cgtttcgctg cgtcttctga atcgctagct ttttgcaaca tttgaagtcc atctctcagc
    18121 cattgaatcg aagaaccagc cacaaaaata cttccttcta aagcatagta aacctttcca
    18181 tttatgccgt aaccaattgt cgtcaataaa ttatttttgg ataattgcgg tttttcaccg
    18241 gtattcatga caataaatga acctgtacca taagtattct tgaccatgcc tggttcgaat
    18301 gccatttgtc cgaataatgc ggcttgctgg tctcctgcca tccctgaaat tggtgcttcg
    18361 cttccataaa aatgataacc ttgcgtataa ccataaactt ctgagttaga agtgactttt
    18421 ggtaacatca ctcgaggaat atttagtaaa tctaaaatct cctgatccca atctagctga
    18481 tggatattga acaacatcgt ccgactggca ttcgagtaat cagtaacatg tgcttgtcca
    18541 tcggtcagtt tccatactaa ccaagaatcg atcgttccaa acatcaattc gcctttttca
    18601 gctcgttctt gcgcgccttc tacatgatca agaatccagc gtatcttcgt tgctgagaaa
    18661 taggcatcga tcactaaccc tgttttttcg tggaaaaatt cttgatagcc ttcatttttt
    18721 aagttatctg caattccagc tgactgtctg gattgccaaa caactgcatg atagattgga
    18781 cgtcctgtat ctttttccca tattactgtt gtttcacgct ggttcgttat gccaatccca
    18841 gcaatctgat ttggtttgat ccctgattcg atgaacgctc cggcaataac tgactgtaca
    18901 gaattccaga tttcatttgc atcatgttcc acccaacttt cttgcgggaa atattgcgtg
    18961 aactcttttt gagaactgcc gatgtgtctt cctttcttgt caaagataat tgctcgtgag
    19021 ctagttgtac cttggtcaat cgccatgata tacgttgatt ctgtcattgt gattccctcc
    19081 attatatgca aacgctttct ttaactactc ttatcatact actgttttaa aaaaagaaac
    19141 tgtcaaaatt ttattctttt tttctttttt tgacagtttt ttattatgaa gctatttttt
    19201 aatcaattct tttctttttt cagcgtctct attcttcttt tcaatcgaat aatgtcgtaa
    19261 ggtgacgcaa attctgacag ataatattca cactgttttt tgtttctctg gcaaaatgtg
    19321 atcaccaaat catattcttt accttcttca taacttccaa tctcaatacc gccgattccc
    19381 ctcagttctc tgatcagata ttgttgaaaa gcaatacgat aaatcggcag actttctaaa
    19441 tcgattccaa tcactactgg ttttgcaata taaaaatcga tcatcaaaag aacattggca
    19501 tactggatca tcaggtaact aagctcacta tcttcagctc ttttcctcgg caattgctgc
    19561 accgctgttt cctgtaattt cgccagtaac tgtgttaagg aatgcccgag cattttctgc
    19621 tgctgttcca aaagccgatc acgctcatag acttcgatct ttccaacaaa gaaaaaccat
    19681 tcattgttga tctgggaaat ttggtagcct actgcctttt cttctttgat tttcaaacga
    19741 cgaggacgat aatgccataa catcgtttcc cgaatgtaag tatctaaaac agcggttggt
    19801 aactttttgc tccgtgtcaa atcatactga taaaatattt cttcgtctac tatcgaaaaa
    19861 gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat tcagttctgc tgctgttcga
    19921 ctaagatgca tcacgatgat tggatcgatt gcttgataaa gccggtccga ctgatagagt
    19981 aatttctttt ctttcaaagt tgcgtacgta cttttttcat tcaatagtct ttttttcgta
    20041 atcccgagcc agcaagcaat ctgtgcttct gctgatgccg taaacgttgt ttctaataca
    20101 cggtccaatc ctagaatgaa cggtcttttt tctggtgtat tttgcaaaaa aagcggtcgc
    20161 tgatcttccg ggatatattg gaatagttca taataaaaat atcgtatctg tagctcttct
    20221 ccgtataatt gaccgttttt gatttgtaat ccaaattcct ctaagagctg gttcaattct
    20281 ttgatctttc gaaaaacaga agactcactg atattaaacg ctgtcgctaa tcgaacgatg
    20341 gatacttgct gatgttctaa taaatagacg agcatcttaa acttcaagct ttcctgcaat
    20401 aaaaacaata gaattttttc caaacttaca tcttcgtcca acacaagcag cgcctgatac
    20461 tcatttcgct gtacttctac ttttttttcc atcattcggc caatcaattc aatatcttcc
    20521 aaatattgat caaacgaggc ttttgacagt tggacatgct ctctcaaacc attgatagag
    20581 gctgttcccc cttgcagaac aaggtttcgc aaaatcagga tctgtaactg ctcttttctt
    20641 tctaataatg cttcaatctt cataggcagt attcgccacc tttgaccaca ccatatctgg
    20701 cacacggtat tctggctgga aaaatagctc atgttcttct aactgttcaa acgtccatcc
    20761 ttcttctact gcaattgtca gttggtcaag aaggtggaac atctcttttt tgctttggag
    20821 ctgtgctcca agaatctttt tttcagaatc aaatagaaat ttgagtttaa ctttgctttg
    20881 atttattgct gtaagcttgt agtctacgta tcctgtttca attttttggg gataaaaaat
    20941 gctctcttct tctgtcaaac ctacgcttcc taagaaatag ccaaaccact gattgccgac
    21001 tggacgatag gttccctgat cctttgtctg tgagccacta attgtcttgc tgacatttcg
    21061 tgctgtacgg acagcgttat ttactagaga aacatacatt ttttcatttg ttggacgaaa
    21121 agttacttgg atcgcatcgc caatcgcata aacgtctttt tgagacgttt gcaagtagtc
    21181 atctacccag atcgttccat catcattcaa tgttaattgt tcttcccaca tctgattgtc
    21241 gggacgagaa tgattagcca gcaaaacaag atcatagttc tcctttcttt tttccgtctc
    21301 tacaatagcg gtttcatttt cggtcaaaga cacaacttgt tcttctaaaa aaagtgaaag
    21361 cccttgattt ttcatttcct ttactaacgg ttctgtcatt tcaggatcta gataacgaaa
    21421 aagaattgac tttcggcttt cgtaaagatg gatttgtttt tttcttgatg ccaacccttc
    21481 agctatttcg attcctgctt gtcctgcacc aataattgca attttctttg cattcatgat
    21541 cttttgctga agagccgtta cgttttgtat ctttttaact gggtgaataa gcgttgattc
    21601 tgcagaaata tttcgaaact tttgatttga accagtcgct agaatcaaac gatcaaaatc
    21661 caattttttt cctcctgcta aaacgacttt tttttctttc aatcctatta ctgtcttttc
    21721 tgtgtaaaca tggattcgat agcggtcagt cagttcttct ttcgtgatcc aagaaagttg
    21781 tttttcactg gtaacctttc cttttaatat caaattgaac ccgctgggaa tccatcctaa
    21841 cttcgattgc ttttcaatca aatatatttc acttgttggc atcaattggc gaagataaat
    21901 agccgcatga atccctgcaa acgacccgcc gacaattaca atgttcatct gcattcctct
    21961 ttcttataag gtgttctact gctaacttct caatcagaaa acacagtatt cataactcgc
    22021 tttcaacctt atgatacata gcgatgcagc agaatcaacc ctctccatca taccgtgttc
    22081 taggtctgct tctcaataat gtactagtgg acctaattga agagtatcat ttctgaagag
    22141 atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg ccttttacgt acctctgtcc
    22201 aaataggaaa cagaaaagaa cgaaaagacg agttttagcg tgtgtaatgt aatagttaga
    22261 atagacacat catttatttt taattcttta atgcttcaac aactgcttcg cctaattgat
    22321 gagcagattg aggattttgc cctgtaagaa gccgcccatc aatttgaacc acttctgaaa
    22381 aagctgctcc agactgatag tttgctcctt tttctttcaa agcatcttct gctaagaatg
    22441 gtacttcatt cgttgtacca tttgcttcct cttcttcatt actgaaccca gtcactgtac
    22501 gtccagctaa aatagatttt ccagactcat ctgtaactgg caacaatcct acaacaccgt
    22561 gacaaacagc tgagaccagc ccttcatttc gatagatttg tgaagcgact ttcccaagat
    22621 ctattgcttc tggaaaatcc cacattgttc cgtggccacc agcataataa accgctgcat
    22681 aatcatttgc atccacatca tcaggacgta aggtatttcc caaggcccga tttctgaaat
    22741 cttcatcttc ataataggcc caatcgattt catccatatt caaaacactt tgcggttcta
    22801 aaggtacata tccgccttta ggactgacaa aatctgcatc gatcccattt tttactaata
    22861 catcataaaa atgagtcagt tcacttaacc acaaacctgt aggacgctcg atcttttcgt
    22921 acttttctac atttgttaat acgatcaata ctttcatttt cccatcctct ttctataaaa
    22981 attgcgttat tgataacgtg ctttcaagaa attatataat gggcttggca aataaagctc
    23041 tttatatgct tcttcaaaag gtttcgttac aaattcatcc tcacggtttt ccaataattt
    23101 ctctgcccag tcaggatcga cgatcatttg ctgtccaatt gctgcaagtt ctgcatattt
    23161 caatacctgc tctacatctt cgcgattgcg gatcccaccg acaccaacta aagggatctt
    23221 tccttgaatc gtgttatgga ccacttcaag aatcgattta tcgttgtatt tatcagaacg
    23281 agctattcga tcatacatat ttaatgaaac atgcagataa tctagttttg actcacgtaa
    23341 tcgttcaaga agccagatcg tgtcttcaaa acggatacct ggtgtttcat attcttctgg
    23401 tgagaaacga taccctacga tgaatggttt tttagcatat tgttcaaccg ctgagaaaac
    23461 cgttgttaat aattcttcaa taaaacgata acgattttcc aagctaccgc cccattgatc
    23521 ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc aaatacgtat ttgctccatg
    23581 caactcgatt ccatcaaatc ctgattcaat cgctctacgt gtggctttgc caaatgcttg
    23641 gattgtttca acgatttctt cttcactcat tgctcgtggt gtttccgcat caggacgttt
    23701 tgctgcaact gcacttgcag atacaggctg ttctccagat aaagttgctc gacttgtcat
    23761 tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct tctccttgga ttgctttcgc
    23821 tagctcactt aatcgaggaa tcatttcatc atgagcgatg ctgagttcac ctggccagcc
    23881 ttttccgata tcttggacat ttgcagcacc agtaataaca gcaccaagac ctttagaacg
    23941 ttgtttataa taagtgatct catcttcggt gatcactcca ttgaaaaagc tgagttgtgt
    24001 cgtcataggc gacagcatca atcggttctt caattgaact cctgaaggta aagtaattga
    24061 ttgatcaaat tgtgacattg tcattcctcc cataattcgc tctgattaac ctatcgagaa
    24121 tcatctatca ttaaaccact cgattagttg tacatgcaac taatttgctt aaaaaatttt
    24181 attttttttg taacttatct gttgcatcat tcattgtctg ggctaattct ttacttgctt
    24241 catttcccaa aactgctttg tatgactgat gaagttcttc ccagctacca tcgacttcct
    24301 tagcaaattg gcgtccttgt tctgtaagat aaactaatga caagcgacct tcgctaactg
    24361 ttttgacgta tcctttatat tctaattttt caacaaaacg agcaatcgtt gatggggcgg
    24421 aatagagcat ttgtgcaata tctttttgtg gtattccatc tttttctttt aatatcaata
    24481 aaataaaacc ataagttggt gccagacctg tccttctaaa cgcttcatca gctaatttat
    24541 tcaacatgcg atctagcttt ttcacgctaa agaaaagaca ttcatctaac gctttttcaa
    24601 ttggcttttc catttcatcc cctccattgg cgtacatatc atagtatgat ttttattttt
    24661 tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag atttgaaaaa aagaagtagc
    24721 catcttcaaa gaatttcggc ttgttttcga caaaactcct tctggaacac cgtgaatcag
    24781 aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa attttgtgaa aatggctttc
    24841 cacatttttg cataaatttg gcttaatacc gaaaaactgg cttttgaaca ccgtttatat
    24901 cagaggttgt gaaaaagccg tcctgcatca agtaataaga acagccaaac aaaaatagtt
    24961 cctcatattt tgtcgttttt tgagctaatg ccgatggtca gctgattgta caccgtttat
    25021 tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag gagcaatttc aaaaaacagc
    25081 ttctcatgtt ttatgactat ggtgacttgt gatcgaggag ttggcaggag aatactgttt
    25141 attcgtaaag aaggggctgt gacaagactt ttgtcacagt cccttatttc cttttattat
    25201 aggatttcgc catttgattg gatgacttgt ctgtaccaat caaatgattt tttctttaac
    25261 cgtttctttg tgccattccc ttcgtcatct tgatcaacat agatatatcc ataacgtttt
    25321 gacatttgtg aagtagaagc gctgacgata tcaatgcagc cccacgacgt ataaccaaat
    25381 aaatctactc catctttgat agcttccttc atctgctcaa tatgctgctt caaatagtca
    25441 atgcggtaat cgtcttgaac ggttccatct tcttctactt tgtctatagc tccgagtccg
    25501 ttttcagcta tgaacagtgg tttttgatac ctatcatata attgattcaa agcaattctc
    25561 aaacccttag gatcaatctg ccagccccat tcacttgatg gaagatacgg atttttaatt
    25621 cctccagtta ttaagtttcc ttggacccct tcccaatttt ctggatgagc actgactgca
    25681 ctagacatgt agtagctaaa agaaacaaaa tcaacagggt aggctgctaa gatttcttca
    25741 tcacctactt ctatttttaa gttgacacct ttttcttcaa acattcgttg cgtatacgct
    25801 ggataatatc ctcttgcttg aacatcacta aagaacaaag aagttctttg ctgctcaatt
    25861 gttttttgaa tatcatccgg atcacaagaa tacggaacaa gaagctgata gctgatcata
    25921 caaccgactt gtgaaccttt gataatttca tgacaagctt ttgtagctaa agcacttgct
    25981 ataaactgat ggtgtgcacc ttggtatttc gcttgttcca aatggggatg gttttcttca
    26041 attactccta cgctaacaaa agggtgatgt tttacacagt tgatctcatt gaaagtcatc
    26101 caatatttta ctttattttt atagcgtttg aatactgttt cagcgaaacg aacgtaaaac
    26161 tgaataagct ttcgattagg ccatccacca tattctgtaa ccaaatataa cggcatttca
    26221 taatgggaaa gagaaacgac tggctcaatc ccatatttta gacattcgtc aaacacttga
    26281 tcataaaaaa ataatcccgc ttcattgggt gtttcttcat caccatgagg aaaaattctt
    26341 gtccatgcga tagacatccg atagcattta aaacccattt cagcaaataa acggatgtct
    26401 tctttaaagt gatgatagaa atcatttccg tgtcttttag gataataata cttgctgttg
    26461 ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat gccattgatc tgtccatttt
    26521 tctttaggta aatcgctgtc gaatgtatac acatcagaca cagataatcc tttttcatct
    26581 acatcccatg caccttcaca ttgattggcc gctgtcgctc cgccccataa aaatccttct
    26641 ggaaattttt tcatatcgat ccccctaaac ttgctagttt tcttgtttga ttatttttct
    26701 tatgactagt aatgatattt ttgaatctac tactatttgt aacaataacc attgtggtcg
    26761 gatcatatcc ctctttgatt aattcttgca aatctacttc catcaataaa tcgcctttac
    26821 gtactttttt tccatcagat actgcacttt gaaagtattt gccatttaaa ttcactgtat
    26881 caataccgat atggatcagt atctctgctc cttcttcact gactacccca attgcatgtt
    26941 tcgttggaaa aactgttgaa acttccccgt ttacagaact gtagattttt ccgtccgttg
    27001 gaatgatcgc aaacccttct cctaaagatc cactagcaaa tgttggatcg tttacttcag
    27061 ataaatcaat tatttcacct gctgcaggag tacttaacac tatatccttt gaggattctt
    27121 cttcgatttc atccttgtag ccgaaaagaa aagttagcat acaggaactg atcatcgcta
    27181 agaaataact agcaactagc caaacaaatc cttcaccaat ataaatcggg aaagacaaaa
    27241 tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc accagctact ccaccaaata
    27301 tagctccaca tatacaagca atgtaaaatg gtcgtttata tttaagagtc acaccataaa
    27361 cagcaggttc agtgattcca aataatgctg tgataaatgc agataaagat aaacttttta
    27421 ctttcttatt tttcgttttc aaaaataccc caagtactgc tcctgcttgc gcagctacgg
    27481 cagtcataca tgctggacct agcgtatcac gaccgtagaa agaaagattg ttggtcatta
    27541 ctggtacaag tccccaatgt aacccaaaaa tcactaacac ttgccaaaat ccgccgataa
    27601 cagcaccaga taaaacaggg ctaaagttat aaacagctaa atatccagct gcaatcagct
    27661 gacttgcata atctgcaatt ggacctacaa ctaataaaat aattgggacc atcacgatga
    27721 tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg caagcttttg cgaatcttag
    27781 gctcaatata agaaagaacc catacagcta aaattgctgg aataaccgta cttgtatacc
    27841 ttgctagaat cacgggcatc cctagaaatc gcaaggttat gctatcacta tatgcactga
    27901 caatcgtagg ataaacgaga gctgctgcaa taaccatagc gatgaaacga tctgtattga
    27961 acttttttgc agcagtatat gccaaaaata taggcaagaa atagaaaaat gcatcagctg
    28021 ctgcattcaa aatgatataa gttcctgatt cagtagttaa taaattcaaa ctcgtacata
    28081 aggcgagtac tcctttgatc aaaccacttc ctgccaagac gcctaaagtc ggggcaaaaa
    28141 cagcggcgat catgtctata aaagagttta aaatgccttt tttctcagtt cctctctttc
    28201 cactagatgc atcatcttct agtttcattt gtttggataa tacgtcatac acttcattta
    28261 ctgcatttcc tactacgact tgaaacattc ctccagcttc gactacagtg atcacacctt
    28321 ctagttgctc taccttctcc ttatctgcct tcttattatc ttttagggta aagcgtaacc
    28381 ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc tccgacaaat cggacaatat
    28441 ctttcgctaa ttgtgaatag tccattttta cttccccctt gttaattaat gccgtagcaa
    28501 tccttcttac aagataagta tagttcttag aaaacggttt ctccattcaa ttagtacccc
    28561 ttctaatagc gaacgaaact gtacaatagt gtgtatggtt tacttagccg agcaaatcac
    28621 tcactgcttg aaagaaaaca agtatatcgt ttctcttata gtcaaaggag acaaaaaaaa
    28681 cacgcttgat cttcctttgc tgatgtataa gtggtatctc aagctgatac tctgtgattt
    28741 ttgtttctac ttggcttttg ataagacagc acaacaaagt ctcggcattt aacggaagca
    28801 taactaggtc agaactagga gagattagtt gctgaatgga atctatcttt ttatggattt
    28861 ttatttgatt ttcttctttg tagtaatgat caagcagttt tgttttcttt ttttcttcaa
    28921 ctatggtcag cttttcacgc aaaatctgat aaatctccca agattcaaac tcatctagaa
    28981 cactaggtag ataaatcaaa tcatcccact caaaaataag ctcttgctca gtagtcaaaa
    29041 ttgcatcata ttccgttatt ttgtttgatt gatttttgaa aagatacact ggttctatcc
    29101 ggcattcttt gatccacatg ttcatacttt gttgaatctg tttttttagt gtattcaaaa
    29161 tactaattgg ctggttacta acaatcagta tctccccttg ttttcgaaaa ggattcaacg
    29221 cttcggcaag atacaaaaca aaataagcgc tttcagctaa attgggacgt aattgaaaat
    29281 gtattgggaa gaaacgccga attaaataaa tttctaaagg ataacgggag atgatctttt
    29341 gtacaaaatg attcatggta ttatgtccgg catccatcct cgttttcatt tgtttgatat
    29401 gggttagcaa aaattctgtt tcatggaaca ataacgaaac aggaagatct aaaaaacgaa
    29461 taatcgtttg ctcaaatgct cttagattct tctctatttc tttgtttttt tcatcattaa
    29521 tcatcaaata atttaactct aatagtcgat tttcaattgc ttgtttttct tgattggaaa
    29581 aagaataggg tagttctgat gataactttt ttgtgagggt ctctaccatc ggaataaata
    29641 atgtatacct gtttgttttt tctaaaagtt cttctcttcg aagaatggtc aaaacgagat
    29701 accgagaaaa tcgtatgaag tcctctcctg aaacaatata attatatgat ataagaacct
    29761 cttttaatgt cttttggaca gtcggcatga tattggaaaa aattgtactg atttgttgag
    29821 gaaaccttgc gtgttggatc accgtttctg ttccaacaag agataagaaa attctttttg
    29881 aattttcttc agcaatgatt tttagtcctt ttgtcgacga aacttccagt tctaatttag
    29941 gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa tgatacggct gtttttgaaa
    30001 gaaagaaaat gtctgctatc ttttgcatac tgacataccc tttttgaaac aataagtacg
    30061 tcacaatagt tgaagctcgg aaatccatgc tggaatagat tttatgtttc gtcacctctt
    30121 gtatgatttg ctgtttagtt tcagattcaa ttgattctaa aaaataacct gaacgtttat
    30181 ttgactggat aatgattttc ttctcgtaga tagcaagcat atttttgatt tcagtttgga
    30241 tcgttttctt gttggtacct aaagcttcag ctaattcttc gcttgttatc caatgattct
    30301 tatttcttaa tactacaaga atttggagtt gtcgtttatt taagtccatt tgcttacacc
    30361 tcctctttgt tataaacata caggaaaaac ctctcaaatg acaatagtca cttgcagagg
    30421 tttaaaaatc atttgaagaa agatctatat tcaaggtttt taccattaga ctggataatt
    30481 tgtttataat attcaaaact atcttttttc aatctcctaa agcttccgct cccatcattt
    30541 tgtctatcca cataaataaa gccatatcgc ttcctcattt cccctgttcc tgccgatacg
    30601 atatcgattg gtccccacca taagtaacca atgatttggc aaccatcttt tattgcctca
    30661 gcaatttcct gaagatgtat ttttagatac tcacaacgtt ttggatcgtg gattcgattc
    30721 aattcatctg gtgcttcatc tagtccaatg ccattttcaa ctatgaataa tggtaattca
    30781 taacggtctg tcaattcatt catgacataa cgaaacccta gaggatcaat tggccaactc
    30841 caaggttcgg gagatttttc cttcaaaaag ggattatcga aaccatttgc tccgccagta
    30901 tctacgcgca tttctgcttc ttttttatag acagcgctac gataataact aaatgcaata
    30961 taatctgccg tgttagcttg caagcactgt tcgtctccat ctaacattac tggtgcacag
    31021 tcttcttcct tccatgtcct gtaaatataa gctggataat gccctcggca catgacatcg
    31081 gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa atacattctc tggatcacag
    31141 tcatatgcat aagtcgctaa tgcagaaaag ctacacataa tacccatctt agcatttgga
    31201 tcgattttct ttaataattg agtagcacgg gcgttcgcta gaaacatatg atgacaggct
    31261 tgataaatca ctttttttgt tagtgtagca atgggttcaa gtttatctct agcagggaat
    31321 gtaaccaatc cagcagctgc ataaggcata cgaaaagcat tattgatctc attaaaggtc
    31381 atccaatatt tcactttccc ttggtagcgt tcaaagacgg tctgcgcata aaattcaaaa
    31441 aagtcgatta attttcgatt ttgccacccc ccatactctc ctaccaatgc taagggggtt
    31501 tcataatgac taagagtaat tactggctcg atatcatatc gatttaatgt attgattagt
    31561 ctgtcataaa atacgagacc agcctcattt ggcttctgtt cgtctcctct tggaaatatt
    31621 cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta actccttcag taaacgaata
    31681 tcctcttcaa atcgatgata aaaatcaatc gcttcatgag aaagataatg gagtgattcg
    31741 tctaaagcaa tctcccaaat ctttttttct tcattccact gaatactagg atgctggttc
    31801 atgatcccga taagcgtatc tgtcacatta ggtaatttgc cgtcttccaa ccatgcacct
    31861 tcacattgat tagcagctac tgctccgccc cataaaaaat tttctggaaa tatttcctga
    31921 tccataacta tctctcctcg ctatcaacaa tttcttactt gaagtatagc tcgattcatc
    31981 aaaacaagat attctaacta tttcggcaga agagaagaaa aaaattgaat gctactcttc
    32041 atttttaata tttatatata acaaatacat aagacgatcc ccgaaagaaa acactcgtta
    32101 accatctaag caaacgattt attttacttc caacacaaat gcttcataag gtcgtaaatc
    32161 aactttgccg tctggtaact gtttcagagg ataatttgac acaataaccc ttaaagcttc
    32221 acgtggaata gtgaacgtct gcttttcact ggacatatta caaataacga gccattgctg
    32281 gtcttcatat atgcgagaat aggcaaagac atgctcaggc gtcttttcca ctaattcata
    32341 gtctccccag acgatgatgt catgttttct acgcaaatcg atcaatttct tgtacatata
    32401 aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg atttctttat aatttggatt
    32461 gactcgaagc catggaattc cagtagtaaa cccagcatgt tcacgggcgt tccattgcat
    32521 aggcgtccta gcattgtctc ttcctttcgc cttgattgat tccaagattt cctcttttgt
    32581 aaatccagaa gaaatccttt cgttatacat attgattgtc tcaatatctt ttgcttccgc
    32641 gatatcatcg attgggcaat tcgtcatgcc taactcttct ccttgataga tataaggggt
    32701 gcctttcatc atatggagca agatagcaaa catcttggca ctttcttttc gatacacatc
    32761 gtcatttccc caacgagaaa caattcgcgg aagatcgtga ttattccaaa ataaactatt
    32821 ccatccttca tcacctaaag atgtttgcca tttggataat acttttttca gttcagagat
    32881 actcaagggt ttcaaatccc atttgtcttt gccttcttgc tgatctaatc ctatgtgttc
    32941 gaattggaag accatggata attcatttct tttaggattg gaataaagtt tagcgatttc
    33001 ggtagttgct ccccatgttt cgccaacagt catcagattt ttgtcaccaa aagttgcttg
    33061 attcatttcc tgaagatact catgcagttt aggtccatta cttgtgattt ctttatccgg
    33121 ttctttgcca atcatatcga tcacgtccat acgaaagccc ccaatacctt tatcgatcca
    33181 gaaattcatc atttgataaa tctcttggcg aacctgttta tttttccaat tcaaatcagg
    33241 ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt ttttcatcca actgccaagc
    33301 agaaccactg aatattgagc gcaatctatt aggctctttt ccattgaccg gatcacgcca
    33361 aatataatag tcccgataag gattttcttt atttttacgg gcctcaatga accacggatg
    33421 ttcatcagaa gtatgattca ccaccagatc catgatgata cggatatttc ttttctttgc
    33481 ttcttcaatc agttcttcca tttcttccat cgtaccgaat tctgccatga ttgcttgata
    33541 attactgata tcatacccat tgtcatcatt cggagactcg tatactggag aaagccaaat
    33601 tgcatcaata cctaaatacg ccagataatc caacctggaa ataatccccc gcaaatctcc
    33661 aataccatct tgatttgaat cttgaaaact tcttggatag atttgataaa caactgaatg
    33721 gtgccaccat ttttgttcca tgattttcct cctataaatg gatgtttcta ttttttgctt
    33781 aatcatttat tttagtctct ctcactaaaa attgtaacgc tttcaaatag ctattgcacc
    33841 ttgttatcgg taacagaata aaacaaaaaa acagctgagg aaattccctc aactgttttt
    33901 tgaaaggatt gatttatgca gggaagaatg ctgatttcac aacttcttta taaacggtgt
    33961 tcactcagct gtcccttggt attagctcaa aaaacggcaa aatataagaa actatttttt
    34021 cgtttttttc tctaatactc aggccagaac gctgatttca caacctcttt atttcacatt
    34081 caatatccac atgaatccca caaatacaaa gaacagacca tacatgattg gagatacttc
    34141 ttttcctctt ttagctgcta acatggtaat tggatagaag ataaaaccta atgcaatccc
    34201 atctgaaata ctataagtca aaggcatgcc gatcaagatc aagaatgaag ggatcgcaat
    34261 ctctaagttt ccccattcga tttgtcgcaa agattgtgcc atcaatactc ctaccataat
    34321 caatgctggt gcagttactt gtgaagtaac aactgatagt aatggagaaa agaacaagcc
    34381 taacaagaag aaaatccctg tagtaatagc tgtgatacct gaacgtcctc caacggcgat
    34441 accagctgat gattctacaa atgcaccaac aggagaagta ccaaataagg aacccgcaag
    34501 catagctgtt gaatcagaag ctaaagcttt ccctacacga ggcatcttgt tgtctttcat
    34561 gaaacctgct tggtttgcta atccaactaa agttcctgct gtatcaaaga aagtcacaag
    34621 caagaatgtc agcactacga cccacatttg aagagaattg atgtccccta catgttttaa
    34681 tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca acaatttcag aaggcattgg
    34741 aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc atcccgatga agatgccacc
    34801 tggtacacgc cgaacaagca agatagcagt tacgactaat ccaaaaatcg tcaaccaagt
    34861 agaaccaacg ttgagtgatc ctaatcctac taatgtagat tcattcgaaa cgatgatccc
    34921 accttcgctc agaccaagga aagcgataaa tagaccgata ccgccagaaa tcgcatattt
    34981 caaatctgct gggatcgcat caatgatcat ttcccgcagc ttgaagattg tgatcaaaat
    35041 aaaaatcaaa gaagcaacga atacgccggc taatgctgtt tcccatggaa tccccattcc
    35101 taaacaaaca gaataagcaa aaaaagcatt gatcccaaga gcaggtgccg ttccaattgg
    35161 ataacgggct aataccccca ttaaaacaca gccgatcgca ctggctaatg cagtcgcagt
    35221 aaacactgct ccttcatcca ttccagaagc acctaataca gttggattta caaataagat
    35281 ataagccatt gagataaatg ttgtaaatcc tgccagtacc tcacgtttta ccgtggtgtt
    35341 caatttttct atttcgaagt aggaaagtat tttttccttc atcttccatc cccctaaatc
    35401 cgaacattat aaataaagtc cgttaaaatt tcgatttatt aataaaacaa cgtgttttat
    35461 catatactaa aacaaaacat aagtaaacaa aaaattcgga ttttaagcaa aatccgaacg
    35521 ttccatctga ttaaatccct ttatctcaaa gttcatgaac attcactttg gattacaaga
    35581 atttctttat tgtgttaata aagtttcttc attctagtct caaaaatcaa tagtgtgatt
    35641 tgattttacc atttcgtttc tgttcttctt tgatgaattc ttttgtatcg acaggattca
    35701 gattatttag tccgccaaaa cctgtatcga ccatttttcc ccagcctttt gtcttctttt
    35761 ttcctgattt gagattcttt gctcgaagct tctcactatg ttttgctcgc cgatacttca
    35821 aataaccttc taatcttccc attagatcca ctgtttttcc tcctttttat tcaatcagac
    35881 gactcagctt ttcctataac aaaataattg ccttcagggt cagaaaaatt gaaagtcaag
    35941 atgccattgt tatctgtgat ttctcctggt tcttccatgt tctcgtagat agtttcaaat
    36001 tcatccgtaa aaaatactaa agaaggcggt ggtcctaata cctctggtga aaacttctgc
    36061 acaaatactt ttgggaatac gcctaattct aattcttttg agatgctgag tacaatactc
    36121 ttaaattctt ctggcaattc gattgtttca gaaatttctg caccaatttt ttctacaaag
    36181 aatcggcaaa tcatctctac gtcattgaca taaagcatca cacgtgtttt ttgtatcatc
    36241 atttgaccct tctttctatt tgattttctt tcctgtctat aaactgattc cattatatca
    36301 cgaaagcgca aacaccttgt gtttgatact attcaagaaa aataaagcaa tagtttctag
    36361 gattgctttc ctccacaaac tttttttaca aaaaaaaaac ggcaatgaat gccgctcttc
    36421 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
    36481 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
    36541 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
    36601 gtcagcatcg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
    36661 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
    36721 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
    36781 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
    36841 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
    36901 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
    36961 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
    37021 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
    37081 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
    37141 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
    37201 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
    37261 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
    37321 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
    37381 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
    37441 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
    37501 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
    37561 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
    37621 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
    37681 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
    37741 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
    37801 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
    37861 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
    37921 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
    37981 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
    38041 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
    38101 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
    38161 tttattttgt ttgtaagact agttcacctc tttttgttgc aaatgcaact attttcaata
//
LOCUS       DAYYKN010000011        34306 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000011 DAYYKN010000000
VERSION     DAYYKN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.011"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..883)
                     /locus_tag="ACHBB7_000474"
     CDS             complement(32..883)
                     /locus_tag="ACHBB7_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM9208454.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(896..1681)
                     /locus_tag="ACHBB7_000475"
     CDS             complement(896..1681)
                     /locus_tag="ACHBB7_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9208455.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(2033..2974)
                     /gene="ribF"
                     /locus_tag="ACHBB7_000476"
     CDS             complement(2033..2974)
                     /gene="ribF"
                     /locus_tag="ACHBB7_000476"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM9208456.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(2978..3901)
                     /gene="truB"
                     /locus_tag="ACHBB7_000477"
     CDS             complement(2978..3901)
                     /gene="truB"
                     /locus_tag="ACHBB7_000477"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM9208457.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(4269..6631)
                     /locus_tag="ACHBB7_000478"
                     /pseudo
     CDS             complement(4269..6631)
                     /locus_tag="ACHBB7_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            6796..7252
                     /locus_tag="ACHBB7_000479"
                     /pseudo
     CDS             6796..7252
                     /locus_tag="ACHBB7_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(7409..7756)
                     /gene="rbfA"
                     /locus_tag="ACHBB7_000480"
     CDS             complement(7409..7756)
                     /gene="rbfA"
                     /locus_tag="ACHBB7_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM9208458.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(7783..10089)
                     /gene="infB"
                     /locus_tag="ACHBB7_000481"
     CDS             complement(7783..10089)
                     /gene="infB"
                     /locus_tag="ACHBB7_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM9208459.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(10102..10413)
                     /locus_tag="ACHBB7_000482"
     CDS             complement(10102..10413)
                     /locus_tag="ACHBB7_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM9208460.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(10410..10703)
                     /locus_tag="ACHBB7_000483"
     CDS             complement(10410..10703)
                     /locus_tag="ACHBB7_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM9208461.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(10725..11900)
                     /gene="nusA"
                     /locus_tag="ACHBB7_000484"
     CDS             complement(10725..11900)
                     /gene="nusA"
                     /locus_tag="ACHBB7_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM9208462.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(11923..12396)
                     /gene="rimP"
                     /locus_tag="ACHBB7_000485"
     CDS             complement(11923..12396)
                     /gene="rimP"
                     /locus_tag="ACHBB7_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM9208463.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(12541..16899)
                     /locus_tag="ACHBB7_000486"
     CDS             complement(12541..16899)
                     /locus_tag="ACHBB7_000486"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM9208464.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(17111..18820)
                     /locus_tag="ACHBB7_000487"
     CDS             complement(17111..18820)
                     /locus_tag="ACHBB7_000487"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM9208465.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(18888..20156)
                     /gene="rseP"
                     /locus_tag="ACHBB7_000488"
     CDS             complement(18888..20156)
                     /gene="rseP"
                     /locus_tag="ACHBB7_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM9208466.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(20317..21117)
                     /locus_tag="ACHBB7_000489"
     CDS             complement(20317..21117)
                     /locus_tag="ACHBB7_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM9208467.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(21114..21926)
                     /locus_tag="ACHBB7_000490"
     CDS             complement(21114..21926)
                     /locus_tag="ACHBB7_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM9208468.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(22121..22678)
                     /gene="frr"
                     /locus_tag="ACHBB7_000491"
     CDS             complement(22121..22678)
                     /gene="frr"
                     /locus_tag="ACHBB7_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM9208469.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(22681..23403)
                     /gene="pyrH"
                     /locus_tag="ACHBB7_000492"
     CDS             complement(22681..23403)
                     /gene="pyrH"
                     /locus_tag="ACHBB7_000492"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM9208470.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(23539..24420)
                     /gene="tsf"
                     /locus_tag="ACHBB7_000493"
     CDS             complement(23539..24420)
                     /gene="tsf"
                     /locus_tag="ACHBB7_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM9208471.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(24519..25301)
                     /gene="rpsB"
                     /locus_tag="ACHBB7_000494"
     CDS             complement(24519..25301)
                     /gene="rpsB"
                     /locus_tag="ACHBB7_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM9208472.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            25660..26139
                     /locus_tag="ACHBB7_000495"
     CDS             25660..26139
                     /locus_tag="ACHBB7_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9208473.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            26356..27447
                     /gene="phnW"
                     /locus_tag="ACHBB7_000496"
     CDS             26356..27447
                     /gene="phnW"
                     /locus_tag="ACHBB7_000496"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFM9208474.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            27440..27568
                     /locus_tag="ACHBB7_000497"
     CDS             27440..27568
                     /locus_tag="ACHBB7_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM9208475.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            27572..28117
                     /locus_tag="ACHBB7_000498"
     CDS             27572..28117
                     /locus_tag="ACHBB7_000498"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM9208476.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(28571..30262)
                     /gene="argS"
                     /locus_tag="ACHBB7_000499"
     CDS             complement(28571..30262)
                     /gene="argS"
                     /locus_tag="ACHBB7_000499"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM9208477.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(30682..31629)
                     /gene="arcC"
                     /locus_tag="ACHBB7_000500"
     CDS             complement(30682..31629)
                     /gene="arcC"
                     /locus_tag="ACHBB7_000500"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM9208478.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(31744..32763)
                     /gene="argF"
                     /locus_tag="ACHBB7_000501"
     CDS             complement(31744..32763)
                     /gene="argF"
                     /locus_tag="ACHBB7_000501"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM9208479.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(32854..34083)
                     /gene="arcA"
                     /locus_tag="ACHBB7_000502"
     CDS             complement(32854..34083)
                     /gene="arcA"
                     /locus_tag="ACHBB7_000502"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM9208480.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         9197 a         7385 c         6054 g        11670 t
ORIGIN      
        1 tttaataaat gaaattttac catctttatc ctcaaataaa gaccgtgacc atgcttccta
       61 ctacgatcag caataatccg ataatcgtta attttttttc ttttggtgtc ttattttcat
      121 gcaggatgta cataccgcct aatgtagaga taactacgtt tagctgtgta atgatatatg
      181 cagtagcaac cccattttca tttgctgaaa aaatataagc caatgctgac acactaaaga
      241 tggctccaac gatcgttgct ttccaactct tgtcttcaga aaatgcggtt ggtttacgga
      301 aaatcacata aatcactgcc cctaaaaaga caccgatcat ttgcggaaag aaaatggaaa
      361 gtccgcttgc atcaactagt ttaggcaatg cactgtatac ccaataacca atacttgttg
      421 atgctaatag actaattcct tttgtgatac cgcctttttt tccaggatca tcactgattg
      481 ccgttaaagc agaaccaaat acaagaagaa tgattgctgc tgctccaatg attttcccca
      541 tacttccaga ccattctcca aaagcgaaga caccaatcaa cgaggttccg actaactgta
      601 atgcagtcga aattggcatc gttttagaaa caccaatcaa gttgaaagat gtatactgtc
      661 ctgtttgtcc aataacccaa aatgcaccag aaagtaaact aatcaaaaat gtagtcactg
      721 aaataccagc tggagaagcg aataattgaa cgagtaagcc gacaagcacc gctccaatac
      781 cagttcctaa tatctcattt gttggtctgc caccaatctt tttcaaaatc agtggctgga
      841 tcccccagcc aactgccggg atcaatgcaa tcaataaacc catttttatt cacccttatc
      901 cttttccacc ttggaaagaa ggatataaag tcatcccacc atctgcaaat agtgtaatac
      961 ctgtaacata actggattcg tctgaagcca accatgcagc tgtcgccgct atttcttttg
     1021 gttcacctac gcgtcctagt gggatcatac tttccaagag ttctttttgt tcaggatcat
     1081 caaattttgc cgcattaata ggtgtacgga tagcaccagg gcctatacta ttgattctga
     1141 ttcccttcgg tgcatactcc attgctaccg tttcggtaaa taattttacc ccgccttttg
     1201 aagcagcgta atgagcaaac gttggccatg ggatctgttc atgaacagaa gacatattga
     1261 tgatatttcc ttttttatta ttctctgtaa agtaatttaa agccgctttc gttccgataa
     1321 aaacgccagt caaattgact tttaacactc tttcccaatc ttcaaggcta agttcatggg
     1381 ttggatgctg attttccatc ccagcattat taacccaaac gtccagtgtc ccaaaatttt
     1441 ctaaagccgt atcaagtaaa cgctgtattc cttcttcaga actgacatct gcttgtacag
     1501 agactgcttt tccgcctgcc tcttcgattg ctttgactgc tttttctgct ccttctttat
     1561 ccgagttata gttgacaact acattcattt tttctttccc gaatcgttga gcaatcgctg
     1621 ttccaatccc ctttgagcca ccagttacta ctgcgacttt gttttctaat tccttgtaca
     1681 tagtacccac tcctttttca tacctaaacc tgttattacg gataactttt atttccgcct
     1741 taaagtgagt gtacaccaag atatatagtc taaagcaaat gatacgctct agcaatcgtt
     1801 ttcatgaatt gctagtgtcc ttttatttct ataaagaaag aggttgtgac agaagtggtc
     1861 agcttcaaga aatttcggct tatttccgaa gaagctactt ctgaaaaacc atttatttgg
     1921 aaaaaaaggc tgtgacaaga cttttgtcac agcccctttc ctgggcttat ttatcaccca
     1981 ttttattgta attgctagtt atacataaac aagagatttt atgcataatg ttttattgct
     2041 gagaaaaata ttcagctgtt gcttgttcat cctccttgag ttgttcaatc aattcctcta
     2101 cactagaaaa tttcacttga tctcttaaaa attgattcca gcgtaccgcg acatgttcac
     2161 catagatatc ttgattaaag tccaaaatat acaactctac agtcagctcc cgtccttcac
     2221 cgaatgtatc attatgtcca attgaaccca tggctggata ccatgtttcc ccaattttta
     2281 tttccgacac ataaacacct tctttaggca aatgaaccgt gctctttact ttgacatttg
     2341 ctgtagggaa tcctagtagt ctgcctcgtg catcgccatg gacaactacc ccttctacct
     2401 catatgtgca acctaatagt tttgctgctt cttttacttg tccgctgtct agtaatccac
     2461 gaatacgggt agagctgatt ttttcacctt gttcttcttc tttagaaact gtgacgattt
     2521 tgaatcttcc tttggcatat tcaggaaaat gagcgacatc tgcaatgtct tttggtccat
     2581 acgtataatc aaaaccggaa acagcatatt ttgcatgtaa ccctacgatg tattgatcaa
     2641 caaattcctg cggtgctaaa tgagcaaaag aagaagtaaa atcaacttca tataaataat
     2701 ctatacccaa agcggccatt ttttcttctt tttgcttaag gctagtcaaa tatttcacgc
     2761 tgctaggatc gatcttttta aagacaatag atggatgctg attgaaagtc atcaatgcta
     2821 atttcaatcc ttctttttgt gcaatttcca tacctgtctt tatgactttc tggtgtccga
     2881 tatgcacgcc gtcgaaaaat cccagcacta aaacgacatc gtcttctgga atttgttctg
     2941 attggtaagg atgtctgatc ttgatgatat tcattcgtta ttcctcattt ctcaatactt
     3001 tactaggttt taatttgttt ttttctttcg ggtttggctg ataaatactg acaacttttc
     3061 ctttataaaa caaagcaatt tcttctgacg gcatttctgg gagcgcaaat acagtataat
     3121 caagtctcat accgtttttt actttctgcc atacttcatc agtaatatct actcgtttga
     3181 accgttccac tcctttttct attggcagca aacaatcatc gattgtccca tttttcacag
     3241 cttgagctac ttcttctaat gtcagagcat ccttctggga cataccggca cttgctgttc
     3301 gggtcagatc agacatgtgc gcagggtaac caagtttctt gcctgtatct acagctaatg
     3361 tgcggacata ggttcctttc ccgcattcta cttcgaagcg ccaagaaatg gttcctgtca
     3421 cttcgtctct tgtcaattca cttgtccgga taaaacggta aatctgagcc tttctaacag
     3481 gacgctccac tgtttcatta ttgcgggcat attcatataa ccgcttaccg ttgactttta
     3541 ccgctgagta cattggtggt atctgggtga tctcaccaat aaatgaagcc atcacttcat
     3601 ctattttttt ttcagataaa gacatatcta ctggttttgt ttctacccgt tctccggatc
     3661 gatcttccgt cgttgtactg aaacctagag ttatctcgcc tacatagatt ttcccagaat
     3721 ctgtcaaata ttcgataact tttgtggctt ttccaataca aatgggcaac acaccgtcta
     3781 cgtctgggtc aagcgtaccc ccatgtccga tttttttcgt atgtaagatt tttcttaatt
     3841 tgaatacgca atcatgactt gtcattccgc gttctttcca taaaggtaat aacccgtcca
     3901 tgtcttttct ccatttcatt tttgatttaa ctgaattatt ataacacatt gcatcaatta
     3961 ctgacgaatt tgacatcata atgaacggtt tttatttttc ggcaataaaa acgtaacaaa
     4021 attcatgctc acgtaaatag aaatcatgtt atgctttttt aaaggaggga atagaatgga
     4081 agattttttc tttgcaccat ttgattggat ttatgggtgg cttacaggta aatagtttgg
     4141 ttttcacttc ccaaaaacga aaaaaaagac tggccattct tccagtcttt tttccgtttt
     4201 ttttatttat tttttcagtt tcttaatcaa tgtaaaaaat tctattttgc gggatcgatt
     4261 ttatcatttt attggtcaag atttttccat ttccaatcca atacttctgg cagatcaata
     4321 ccattttcac gaatatagtt ttgatgttct tgtaatttct gatccatttt tttcgcaaaa
     4381 tcttctgctt gacttccttt gatttttaaa gctgcgtctt ttgccaaatg ataacgatca
     4441 agttcattta ctacacgcat gtcaaatggt gtcgtgatat ctccattttc tttgtagcca
     4501 tgaatcatta aatgatggtt atgacgatca aagaaaatcg tgcggaccaa tgtttcataa
     4561 ccgtggaaag caaataagat cggtttgtct tttgtaaaat attgatcaaa ttcttcatcg
     4621 ctcaacccac gcggatcttg acttggatga cgtaatttca ataaatctac gacattgatg
     4681 aaccgaattt ttagttttgg gaattcttcc agcagcaaag ttacagctgc taatgcttct
     4741 aagttagatt ccgttcctgc agcagcaatg accaattcag gttcctcctc ttttgctgtc
     4801 gaagcccaat caatgatttt caagccttca ttcactaaga cggctgcttc ttctgctgag
     4861 taaaattgag cacgcggatg tttggaagag atgatcaaat tgattttttc tgacgagcgg
     4921 aatgctttat ccatgactgc gagcaatgta tttgcatccg caggcagata ttcacggatg
     4981 aactccgcct tcttttctgc caaatgtgtc aatattcctg gatcttggtg gctatatcca
     5041 ttgtgatctt gctggaagac tgttgagctt gcaatgagat ttagcgaagg ataatcattt
     5101 cgccaagaaa ggtcatgtga tttccgcatc catttgaagt gctgagtcaa catagagtca
     5161 accactcgca agaatgattc gtaacttgca aaaaaccatg ccgtccagtc agaacgtacc
     5221 cttctaagaa cccttcatct tggtgttccg ataattgact gtcgatgacg cgaccagatg
     5281 gcgaaagcca ttcatcattt tctggttcga ttttttccat ccattggcga tttgtcgttt
     5341 tgaaaacttg attcaatcgg tttgattttg tttcatctgg tccaaatata cggaaatttt
     5401 ctggattttt ctcaatgatc tcgcgaacga atttaccaag ttcggtcata tcttctgctt
     5461 taacggagcc tggtttttca aattgcaaag tatatttttt ccaatctggc ataataagcg
     5521 gtcttggatc gattccacca ttggtgatag gattttttgc cattctttgg tcaccagtag
     5581 gcgcgatttc tctgatttct tctaaaatac gtccttgtgc atcaaataat ttttctggtt
     5641 cataggattt cagccaacgg agtaaagcat ctgcatgatc catatgttct tgatctacag
     5701 gaatcggtac ttggtgtgct cggaaggttc cttcaattgg ttccccgtcc cattcttttg
     5761 gaccggtcca tccttttggt gtgcgtacga tcaatactgg ccattttggc atcacgacat
     5821 ctgttgctgt cttcaggcga gcttcttttt ggatggattt aattttctcg attgcttgat
     5881 ccattttttc agccataaca ggatttaatt tttctgaatc atttccttca ataaagaaag
     5941 gttcccagcc aagtccgtta aagtaatttg ctaattctcc atctgattta cgtgccaaga
     6001 tcgttgggtt ggctattttt gcaccattta aatgaaggat cggtaaaaca gctccatcta
     6061 tcactggatt gataaagaca tttgagaacc agcttccagc taaaggaccg gtttctgctt
     6121 caccatcacc aatcacgaca gcagagatta cttcaggatt gtctaataca gctccgactg
     6181 catgtgaaag agagtatcca agctctccgc cttcatggat agaacctggt gtttctggtg
     6241 ctgcatgaga agcaatgcct ccaggaaatg aaaatcgttt gaataatttt tgcattcctg
     6301 tctcatcttc cgttacttct ggatagatct ctgtgtaact tccatctaaa taagcattcg
     6361 ataccatgac ttgtccgccg tgtcctggcc cttcaatata aaacatgttc aaatcatatt
     6421 tattgatgac tcggttcaaa tggacataaa taaaattttg gccaggaatc gttccccaat
     6481 gaccgattgg atgttttttg acatcttcgg gtttcagtgt acgtctcagt aaggggttat
     6541 ctttaagata aagttgccca acagaaagat aattagcagc acgccaccaa gccgtcattt
     6601 tatcaaagta ttctttacta gaataatcca tacataacac tccttttcat tattactctt
     6661 attctagcgc agatactccc aattgaaacc taataagcac ttctgttcat ttgtttggat
     6721 tttatatcta gccaaatgaa caggtataaa aaaccacgaa atttttcctt tcgctgacgt
     6781 tttttacatt gatccaaaaa acttgtcgtt atcacaatgg ctgttccgca gttcatttcg
     6841 ctcctcatca tgcataattt gctgaatgac gctgggccag ttaacacatt gctggaaaac
     6901 tggggctgga tcgagcaatt cctttcctga ttgatccgtt ttatgtaaaa gtctctgtta
     6961 tctttgtcaa tatgtgaatt ggtatcccag ctactatgct aatttctaca gggattatcc
     7021 aaaatttgga tcgtgaccaa atcgaagcgg cgtaatcgga aatatcaata acttcaacgt
     7081 gatttttctt ttaactgtca acggaccgac aaactcaaac ttttatggtg caggttccac
     7141 tgatcttcta gttacttggc tatacctgac tgtcactaaa atagattaca atctagcttc
     7201 cgttatcggt atcttgatct ttatctatat gcaaaaattt atatactact agaaaaaaga
     7261 aagtcgaatt ttttactttt ctttttctct tttttggtta gctgacacgc cgcaagacta
     7321 aagtcatgga actcttaggt aatacgtctt aatgaaaaca aaaaaatccc gcaacccctg
     7381 atttcacagg atttgcggga gtttttgatt agtctttgtt caagtcgcga agcaattcat
     7441 cgatatggtt gccatagaca actgattcat caagttcaaa gaacaattca ggtgtcttat
     7501 aaatacttag attgtgccct aactctcgac gaatcaatcc tgatgcttta ttcaatccat
     7561 cttgtgctgt tttcttatct gatgctaagt cagataaaat gctgtaatag atcgttgctt
     7621 gctgtaagtc ccccgtaact ttcacatctg tgatcgttac gttctccaca cgagggtcac
     7681 ggaccttttt gcgcaaaata tcattgactt cttttaagat ttcttgaccg actcggcggt
     7741 cacggtagtt agccataatg attggcctcc ttttatcagt ttttagtcat ttttgacttc
     7801 ttccatgata aatccttcaa ttacgtcatc tactttgatg tcattgaatt tttcaatcat
     7861 tgcaccacat tcataaccca ttttgacttc tttcacgtca tctttgaatc gtttcagact
     7921 tgcaagttgg ccttcataga ttacgattcc gtcacggatt acacggacac cgctatctct
     7981 acgaatatat ccttctgtta cataggcacc tgcaatcgta ccaactttag aaactttgaa
     8041 ggtttcacga acgatcattt gaccggtgat tttttcttca aattcagggt ctagcatccc
     8101 tttcatcgca gtttcgattt cttcgatggc tttatagatg atacggtgaa gacgaatatc
     8161 gacttcttca gtatcagctt gcacttttgc ctgcggtgta ggacgaacgt tgaaaccgat
     8221 gataatagcg ttgctggctg ctgctaaggt tacatcactt tcattgattg ctccgacagc
     8281 tgaatggacg atttttacac ggacaccttc cacatcaatc ttcttcaatg atgcagccag
     8341 tgcttctgct gaaccttgta catctgcttt gatgattacg ttaacttctt tcaattcgcc
     8401 ttctttcagg ctttcaaata agttatccaa agtcacacgg cttgtggcag cacggttttc
     8461 taataatgca cgtttcgcgc gttcttcacc cgctgcacgt gctgtctttt cgtcttcgaa
     8521 gactacgaaa cggtcacctg cttgaggtac atcattcaag ccagtgattt ctactggcgt
     8581 tgcaggtcct gcttctttat cgcgacggcc aagatcgttt gtcattacac ggacacgtcc
     8641 gtaagtatta ccgacaacga ttgggtcacc aacatgaagt gttccttctt ggacaagtaa
     8701 ggttgacaca ggacctttac ctttatcaag acgtgcttcg atcactgttc cgattgcgcg
     8761 ttgtgttgga tctgctttta aatcttctac ttctgcgact aacaggatca tttccagcaa
     8821 ttcttcgatg ttttggccga atttcgctga gatctcaaca aagatcgttt ctccacccca
     8881 tgcttcagga attaactcat actcacttaa ttcttgcata acatgttgag ggtttgcacc
     8941 tggtttatca attttattta cagcgacgat gattggaacg cctgcagctt tggcatggtt
     9001 gattgcttca actgtttgtg gcatgacgcc atcatctgca gcaacaacta agatcgtgat
     9061 atctgtgata cttgctccac gtgcacgcat acttgtaaag gccgcatgtc ctggtgtatc
     9121 caagaaagtg attggtttac catcaatatc gatctggtag gcaccgatat gctgtgtgat
     9181 tccgccggct tcgccgcttg ttacacgtga atggcgtaat gtatccaata aagttgtttt
     9241 cccatggtct acgtgtccca taatcgttac gactggcgga cgtgagacta atttatctgg
     9301 atcgacttct tctgcctcga agaatttatc gatatcagca atgtcaactt ggactttttc
     9361 ttgtggttcc atgccatagt ccgtcgctaa cagttcaatc gtatctttat ccagtgcttg
     9421 gttttgattg accatgacac caagcataaa taatttttta atgatttctg ctggctcacg
     9481 atgaattttt ttcgcaatat ctgctacgtt cataccttca gtatactcta atacatctgg
     9541 caattcacgg aatttgcgtg gtggtacagc tggtttagtt gattgttgct gtttgccttt
     9601 tttacctttt ttattataac gattacggtt attattatta aatttgccgc gattgttatt
     9661 gtttggtttg ttgttattgt tagtacggtt ttgattgccg ccttggctcg ttccttgacg
     9721 attttggctg ctggtatttc ccgtattatt ttggtttgtt gttggttttt ttccttgatt
     9781 cacttgaccg ctcccttgac cgcgatcttg ataactgcgg ttatttttat ttttttgttg
     9841 attgttcgtt gtttcttttt tacgttgatc gttcggctgt tgattggctg gttttctgct
     9901 tgcttgttgt gcagcttgtt gtttcgtgct tgggtttttg aacgcttgtt gcaatttctt
     9961 ctcatcattg ctagtaatcg cacccatatg gtttttcaca tcgatgccaa gttgttgtgc
    10021 tttttccacg acgtctttac tagatttttt gagttctttt gctaattcat agattctttt
    10081 gttgcccatg caatcacctt cctaaccttc aattagctcc ttggtcttct tcgcaaatcc
    10141 ggcatccatt attccgaata ccttgcgagg ttttccgatc atctgcgtga tttctgcttc
    10201 agaaaatagc tcaaagcaag gaacttcata atatgaactc ttatctttta tcttttttct
    10261 tgtattctcg cttgcatcgg ttgccacaaa caccaatttt gctttttgtt tgcggatttc
    10321 agcaatggtc agttcttcac ccgtgattag ttttcccgcg cgcatcgcta gaccgatgag
    10381 attcatggct ttttgccgat taactgtatt cattttccaa ataactcttt acgtgctttt
    10441 tggtgagtga catagtcaag aagttcttga taaaactcat ctgttaatgt tgtttcgagt
    10501 actcgatcga gaattttttt atcccaagct tgttgtactt cttccggctc aagtgagaca
    10561 tacgctcctc gtccaggcat ttttccagtt ggatcgatcg atacttcccc ttcttttgaa
    10621 cgggtgattc ggaccatctc ttttttaggt ttcatttcac ctgaaaccac ggatttgcgt
    10681 aaaggaattt tcctttgttt catctctttg cctccttact aaagttaagc ttctggtttt
    10741 tcttcgaccg tttcattgtt gaatgcaatt gtttcatatt catcgtctgt taaatctgac
    10801 tgaacgattg cttcatcatg cagttcttct gtttcttttt gttgttcttg attttcataa
    10861 aattccgtca tgtcagattc tgatttgata tcgattttat ggttagtcaa tttagcagct
    10921 aaacgtgcgt tttgtccgcg tttaccgatt gctagcgata attggtagtc tggaacgaca
    10981 actgtgcagg cctttggatt tgtctcatcg aagatcacat ccatgacttg tgcaggattc
    11041 aatgcatttg caatgaatac agctggatct tcattccatt cgacgatatc catgttttcg
    11101 ccttttaatt cattgacgat tgcttgaact cgttgtccct ttggcccgac acatgtaccg
    11161 actgcatcga tatttggatc atttgagcga actgccactt ttgaacgatc gccagcttca
    11221 cgggcgatgc tgacaatttc aacgattcca tcatagactt ctggtacttc ttgctcaaat
    11281 aagcggcgca ataggtctgg atggctgcgg ctaacaaata cttgcggacc tttagacgta
    11341 ttttctactc gggaaacata aactttgatt cgatcatgtg gttgataaaa ttcatttggc
    11401 atttgatctt gtttagaaag aactgcctca attttaccaa gattgacata aatataacgt
    11461 ttatcctggc gttcgacgat tccttgcatg atgtcttttt cataagcact gaattcgtta
    11521 taaataatcg ttctttctgc ttcacgtaca cgctgtaaaa taacttgttt agccgtttgc
    11581 gcagcgatcc gcccaaaatc ttttggtgtt acttcaaaac ggatcgtatc accgatctca
    11641 tatgctggat tgattaaaag tgcatctttc aatgagactt ccaactgtga atccatcact
    11701 tcttccgtta cttcttttac cgcatagaca tggatctttc cttttttctg atcaaattcc
    11761 acttcgacgt tttgtgcttg gccatagtga cgtttgtatg cagaaaccaa tgctgcttct
    11821 aaagcatcga tcacgatttc ttttgagatc cctttttcag cttctaaagc gtctaacgca
    11881 tttaacattt ctttactcat tttttctgat tcctctccgt gattaaaatt ggatagctaa
    11941 gcgagctttt gcaatatttt ttcggtcaaa agtcaattct ttttctcgtg tttttatgcg
    12001 tattttcaat gtaagctgtt cagcatcaaa tgattgaaga aatccttcat attgtttttc
    12061 gccatctact ggttggtata aggagacatg gacataatca ccaagtgcct tttcatagtc
    12121 gctttctttt ttcaatggcc gctctgctcc cggggaggat acctccagga aataagcttg
    12181 aggaatcgga tctggatctg ctgcatcaag tttttcactc agtttctcac tgacaaaggc
    12241 gcattcttcg atatcgatac cgccttcttt gtcaataaat actcgtaaga accagctttt
    12301 tccttctttt acaaattcga cttcgacaag ttcaaaattt tgttcatcta aaatcggtgt
    12361 caccatttct gtgactgttt caacgacgct actcaaaagt ttcgcctcct taaaaaatta
    12421 agtgcgtaat tccattaaaa agagtgagcg aaaaatccgc tcactcacag ttagtatcat
    12481 tacctataag aactatacca tactctgctt aaaattacaa gcagacagga aaacagatgc
    12541 ttatagcata tcgaataacg ataattgatt ttcatctggt agatctttca atacgccatt
    12601 gtcgttcata tattcgatca atgtttttga aactttaccg cgggtggcaa gatcttcttt
    12661 ggataaaaat ggaccgtctt ttcttgcttc aacgatttgt tttgccacgt tggttcccaa
    12721 acttgggaca gctctaaatg gtgcgatcaa cgtatcacct tcaataacaa agttaacggc
    12781 atccgattta tacaaatcaa tcattccaaa tttgaatcct cgttccagca tttcattggc
    12841 taactccagc actgtcagtt gattcttttc ttttacggaa gcatctaatc ctttgtccgt
    12901 gatttctttc attgcttgtt tcacagcatc ttttcctttg cacatggaga ctagatcaaa
    12961 gtcgtctgca cgtacagaaa agtatgcaca gtaatacaaa atagggaaat aaaccttgaa
    13021 ataagcgaca cgaagagcca tcaatacgta agctgcagca tgggctttcg gaaacatata
    13081 tttgattttt gaacaagaat cgatatacca gtcgggcaca ttattttctt tcatcgccga
    13141 tagataggtt tctcgcaatt catcagggat cttgttccat tgtcctttac gaacagtttc
    13201 catgatttta aatgccatcc cgctatctaa tccagcatgg atcaaataaa ccataatatc
    13261 atcacgacag ccgattactt ccgccagtgt cgcatctcct cgtcgaatca attcttccgc
    13321 atttcctaac catacgtctg taccatgaga tagtccggaa atttgcagta gttctgcaaa
    13381 agttgtagga tgcgtttctt ctagcatccc tcgaacaaaa cgagtgccga attctggaat
    13441 acctaatgtc cctgttttgg aataaatttg ttcctgactt actccaagta cttcaggacc
    13501 ggcaaatatc cgcatcactt ccgggtcatc cgtagggatc gtttgtggat cgatccctga
    13561 taaatcttga agcatccgaa taacagttgg gtcatcatgc cctagaatat ctaatttcaa
    13621 tacattatcg tgaatcgagt gaaaatcaaa atgagtcgtt ttccattccg aattttgatc
    13681 gtctgctgga tattggatcg gcgtaaaatc atagacatcc atataatcgg gaataacgat
    13741 gatcccccct ggatgctggc cggttgtccg tttgactcca gtagcgcctt ttgctaatct
    13801 gtcgacttct gcacttcgaa actgcagatt attgtccctt tcgtatcctt ttacatagcc
    13861 atatgctgtt ttgtcggcga ctgtaccaat cgttccggca cggtaaacat agtcttcgcc
    13921 aaacaatact ttcgtatagt tatgggcttc tgcttgataa tcacctgaga agttcaaatc
    13981 aatatcgggc actttgtctc catggaatcc taaaaatgtc tcaaatggaa tatcatgacc
    14041 gtctttgttt aagcgtgcac cacattttgg acattgtttt tctggcatgt caaatccaga
    14101 accatacgta ccatcttcaa aaaactcgga atattggcac tctggacaat aataatgagg
    14161 ggctaacgga ttaacttcag tgattcctgt catcgtcgca acaaagctag agccaacaga
    14221 gccacgtgaa cctactagat agccatcttc attactttta tgcactagtt tttgggcgat
    14281 cagataaata accgagaagc cattaccatt aatagaattc aactcttttt ttaatcgttt
    14341 ttgaatgatc tcaggtaacg gatctccgta catttgtttt gcttttgtgt aactcaattc
    14401 tgatatttca tcttctgaac ctggaatctt cggtgtataa agttcatctt tgactggaat
    14461 cacttcttcg cagatatctg cgattttatt cgtattttct actacgatct ctttcgctgt
    14521 ttcttcccct aaaaagtgaa atgcagttag catctcatcg gttgttcgga aatggacatc
    14581 cggcaaacta tgacgattca acggattcgc tccgcccata gaattgatca aaatttttcg
    14641 gtaaatggca tcttcttcgt tcagataatg tacattacct gttgcaacaa cgattttatc
    14701 caaacttttt ccgatttcta ccaaattttg gataatttct tccaaatcat gttcattttt
    14761 taccaattct tgctcgatca aaggcgcata gaccgctttt ggcataactt caatataatc
    14821 gtagaatttt gctcggtttc ttgcctcttc tacccctttt tgcatcatgg cttcgaatat
    14881 ttctccctta tcacaagctg agccgatcaa tagattttca cgcatctttt tcaattggga
    14941 gcgtggtatt cgtgggacgc gttcgaagta ttcgacattc gacattgaaa tcagtttgaa
    15001 caaatctttc agtcctgcct gattttttgc taaaatcgtt acatggaaag gtcgagccct
    15061 tttgtaagaa tctccttctc cgatatgctc atttagctga tcatgataaa gcatgttatg
    15121 attgtccatt gcctctttca caaagatcca tgctaagtga ccagttgctt cagcatcata
    15181 gattgctcgg tgatgctgtt ccaagctgac accaaatttt ttagataaca cacctaatcc
    15241 gaaacgtttg aattgaggat acagatatct agctaattcc aacgtatcaa tgactggatt
    15301 ggccgcttca ggaataccat accttgcgta gctagtattc aagaacccca tatcaaaagc
    15361 cgcgttgtgg gctaccaaaa ttgtatcttt tgaaaattca aggaacatcc gcaatacttc
    15421 ttcttccgac tttgatcctc gaaccattcc atccgtgata ccagtcagat ctaccgttgt
    15481 tcgtgacaaa ggatgtcctg gatcgataaa ttcatcaaag ctttcgatca catttccttt
    15541 atacattttg actgcggcta gctcgatgat cgtgtcatat actgcagaca atcctgtcgt
    15601 ttccacgtca aatacgacat atgtggcttc actcaatgct tcatgtgcat cattataagc
    15661 aattgggaca ccatcatcta cgacattcgc ttccacgccg tataagattt tcacacctgc
    15721 tttttttcca gcactatgtg cttccggaaa ggcttgcgca ccaccgtggt ctgtaatggc
    15781 gatagctcgg tgtccccatt tccctgcttg tgccacaaga tcagagatgc tgtttgttgc
    15841 atccatcgta ctcatattgc tatgaacatg aagctctact cgtttttccc cttcaggagc
    15901 ataatctttg cgaggtgtat gtttgacttc tatgatatct tgcgcattca tgaccaaatc
    15961 acggacaaac gtatcttctt gaatacttcc tcgtaccttt aaccagctac cgacactgat
    16021 cgcatcaaat acttgttcat ctttttcgcc gttcgagaat tttttgacga taaatgatga
    16081 agtataatct gtgattttca aagtcaagat cttgcgtttt gaacgtagtt ctctgacttc
    16141 tttgtcgaat acgtacccct caagtgtgac tcttcgttct tcttcaacga tactgatcat
    16201 tggtgtcgtt ggttcatcgg ctggaatatt tcgtcctagc tggatgtgga tgggtccttc
    16261 aagagctggc gattgctctt ttctttcctt tttcttttgt tcgtggacaa ctagactttc
    16321 agctgcttgc ttcataaagg cttctgcctg ttcttgtttg cgttcttcaa aaagtttcaa
    16381 tactcgttcc gcttgctgct catccacttc tggttcgata cgaaattttg ggaatccgta
    16441 actgacgaac aactcttcta ctaatggcaa gtattgttgc ttcaaataag aaatagctcc
    16501 tgtactgtcg attggcaaga tgactttacg atcttttatc acaggtacct gcgttttcag
    16561 tacttgctgt gctagtggtg tatcgcattg ctgattttcc aaagcttggg cccagtaatc
    16621 ttgcaagagc tgttcatcga atgtctggtc atctgctaag atattcagtt tgacttgtgc
    16681 aatatcttta aaagcgatag tcagatgctg agtcaatgac cgatatagca taataggcaa
    16741 aattttggaa aaatgcagcg taaattccca taaacgcgat ttacgatgga ccactacact
    16801 atccatttca ccagcagtaa gcagtggatg atttttttct gcttcctcta gttggatttg
    16861 ttccaacagt ttttcaaata aatctcgttt ttctgacaaa gatatacgcc ctttctgatg
    16921 agaaacaatg caattgatcg catcttatcc tcttcttttt cagtaccttt tagtatactc
    16981 gttttttctg agttttacat taaaaaattg gaactgcgat tcaaatcgca gttccaattt
    17041 taaaagcgaa tcatcagatt gtcaaaaaca gctgattcag ctatttttat tttctgtgtt
    17101 ttaacgctta ttattcgcct tggttcaaga ggattggtaa agtattcacc aattcttctt
    17161 tacgtacttc aaccatttcg ccagttttct tgattttcac ttctacgatt ccatccactg
    17221 cttttttacc gacagtgatt ctgattggac agccaatcaa atcagaatcc gcgaatttca
    17281 ctcctgcgcg ttcatttctg tcatcaacca gtacttcata gccagctttt gtcatcgctt
    17341 cttctagttc attcgtcagg tttgtttggt attcgtcttt tagattcatt tggacgatat
    17401 gcaaatcaaa tggcgcaatt ccttttggcc agttgattcc ttgttcatct gaatgttgtt
    17461 ctacgattgc tgataataaa cgacttacac cgataccata acagcccata atcacaaatt
    17521 tttctcgtcc attttcgtct agtacagttg ctcccataga ttcactgtaa cgagtgccga
    17581 ttttgaagat atgtccgatt tcgattcctc gagtaaaggc aagaacacca ttgttgtctg
    17641 gtgaaggatc tccttcttgt acaaaacgaa gatcttcata agaaagtaca gtgaaatcgc
    17701 gttctggatt cacattttta tagtggaatc ctgtttcgtt cgctccagta atggcattcg
    17761 ctaaatcttg gacataacgg tctgcatata cttttacgtc atctgaaaca ccgactggtc
    17821 cgacagaacc aaactcagca cctagatatt tttgtgcttc ttcttctgtt gcttcttcta
    17881 aaaagtcagc agataaaaag tttttcagtt ttacgtcatt gacttcatgg tctccgcgta
    17941 ccaaaacaag gacaggttgt tcatccgcaa tgaacaatac agacttgatg attttttgag
    18001 gttcaacaga aaagaaatct gctacttcgt caattgattt tacgttaggt gtagctactt
    18061 tctccaattc aagctgtgtt tcatgcgatt tttttgatac atagtagctt gtagccatct
    18121 ccaagtttgc agcataatca ctttcagtcg aataacagat cgtatcctcc ccgatttctg
    18181 agatcgccat gaattctttt gagtctttcc cgcccattgc gccaccgtct ccgataatcg
    18241 agcgaaattc taagccacaa cgcttgaata cttcggtgta ggctttttcg taatctttat
    18301 atgtctcgtc caaactttct tccgttgcat ggaaagaata cgcatctttc atgataaact
    18361 ctcttccgcg aagcaaaccg aagcgaggtc gtttttcgtc acgatattta ggttggattt
    18421 gataaagatt taaaggtaat ttcttatatg aattgatttc attacgcatc aaatcagtaa
    18481 aagtttcttc gtgagttggt ccaaggatca tttttcgatc attgcggtct ttgaaacgat
    18541 acaagtttgg tccatatgtt tcgtaacgcc cggattcttc ccaaagttct gctggcaaga
    18601 tggcaggcat cagcatttct accgctccga ttttttcaaa ttcttcccgc ataatgcgtt
    18661 tcagtttttc caatacacga tttgctaatg gcagatatga ataaataccc gctgatactt
    18721 ggcggatata tccagctctt agcaagattt gatggcttaa tacttctgca tcatttggaa
    18781 cttcgcgtaa tgttggaatc aacatttttg attgtctcat tctatatact cctttatttc
    18841 ctcaaaatag ttatcgtttg ttattatacc ctatttgtca actttttcta gaaaaagaat
    18901 cgttgaatat cgttccatgt aactagcacc atcaacaaca ttaaaaatcc aaagccagct
    18961 aatgtcaata ttccttcttt ttcttgactt aatggtttcc cgcgaatccc ttcaaaaata
    19021 ttcaagacta atttcccgcc atctaaggca ggaatcggaa gcagattgac gattccgaga
    19081 ttcattgaaa gaagcgccat caaactaata acagttgtta caccttgatt tgctgcttct
    19141 gaagacaatt ggtacatcat gacaggtccg cctaatttat ctaaactgaa acctgtaaat
    19201 agtgatccta aggctttgaa aatttccaat gaaccgctga aagcttgacg cgtccctcca
    19261 atgatcttat ccataaaacc tgtattcatt ggggctttta taccaagttg gccaactttt
    19321 tcgccatttg actctacaga tttcggtgtc acgtcgacag attgcacctg accttctcgt
    19381 tcgatcttaa agtctaatgt tttatccgga tttttcgtaa tgactgttgt cagatcattc
    19441 catgaatgga tttcttttcc gtccacactg actacctcat cattttcttt taagcctgct
    19501 tctgccgcag ctccattagg catgatctct cctacgcgat tagtgttcgt cacttgaaca
    19561 ccaccttgca taaatgccag aatgatgaat aacaggattg ccaaaataaa gttattcatg
    19621 ggtccagcaa agttggtcag catacgctgc cacaatttag ccgattggaa ctgtacgtct
    19681 tttggcgcaa ttctgatttc tgttccgtcg ttttcaataa tcgtcgcatc gtgatcaaca
    19741 gggtaggtta ctacttctgt ttcgtctcca ttcacataac cagtgatagt cagctcatct
    19801 tctaaatcat aacggctcaa ttccatcggg atagcatttg tcaattggat tttcttactt
    19861 aaatttatct tctctacgat gccgtcagaa tttagtaaaa gagaaagagg cattcctggt
    19921 gccatttctg tttcatcatc cccattgcct gccattcgaa cataaccgcc aataggtaat
    19981 aggcgaagcg tatatgttgt cccgtctttt gcttgatgcc cataaatttt cggacccata
    20041 ccgatagcaa attcgcgaac gaggattcct gatcgtttcg caaagaagaa atgaccaaac
    20101 tcatgaacaa tcactaatat accaaaaacg atgataaatg tcagaatcgt tttcatagct
    20161 tttttctcct ttacttgttt tttgtgtttt ttataaaaca acgataaaac actataattt
    20221 taccatagta taccaaagac accaatagta tcaatataat gaaaaataaa aacctgcgtc
    20281 ttttatgaaa ttcacaaaga aacgcaggta tacttactaa ataatgccaa ataaatgcat
    20341 gataggaaat acaaacagca aactgtcgaa acggtctaag attccaccat ggcctggtaa
    20401 gatatttccc gagtctttca catcataatg acgtttgatt gcagattcga ccaaatcacc
    20461 aaattgtccg actgctgaaa aaataatcgt catcagaagc atgacaaaca taccatgatt
    20521 aaatagttct tttgctgggt atagcaataa ataaagaaat gcgactacaa cagcactacc
    20581 aataccaccg agcgcacctt caatcgtttt gttgggcgaa atatcaggac ttaacttatg
    20641 ctgaccataa cgtcgaccaa ccatatatgc tccgatatca gttgcccaaa caataaatag
    20701 accaaatatc aaaacagcca agctttctgt tcgtgcattg acaaaattct ggaaacctat
    20761 tcccacatac aagctgacaa gcacaggaaa acctgcttca tcaatcgtat acgtattttt
    20821 agaaataact gatgttccta aaagaatcat aactataaga taaaataaca taaatgtact
    20881 tacattctct ggaagaaaga aaaaccatct ttcttttggc aaaacaagta acactgcacc
    20941 aacggcggat aaaactcctt caaaacttaa gagtgtcaat cctttcatcc gaaacagctc
    21001 gtaaactcct attactgcta agattgctgc tgtaagctca accgctatgc ctccgatcca
    21061 tataatcgga ataaacaaga ctagcgccac ggctgctgtg accacacgtt gtttcatgac
    21121 tgatcccctt tctcatcttc tgtttttttt acaccgccga atcgacggtc tcgattttga
    21181 taagaggcga ttgcttcttc aagatgcgca ccatcaaaat caggccaaag cgcttttgta
    21241 aaatacagct cactatatgc gatctgccat aacagaaaat tactaattcg ctcttctcca
    21301 cttgtacgga tcatcagctc aggatcttgt aactctttag gcaagaaacc tgtcattaaa
    21361 tgcttagcaa tcaattcatc atcgatttct tctgcgtgta tttctccttt tgctacttct
    21421 tcggcaattt cttggacagc agtcacgatc tctgctctgc ttccataatt caaagcaaaa
    21481 ttcaatacca ttccagtatt gttttttgtt tgctcaattg cacgtcttac agcatcttgc
    21541 gtatgttctg gcaatacgtt ttcataaccc atgacatgaa ctttgacatt ttctttaatc
    21601 aattctggta cgaacgtatc aaagaagtcg acaggtagtt gcatgagaaa acttacttca
    21661 tcttttggtc gcttccagtt ttcagtcgaa aaggcataaa gcgtcaacac tttcactcca
    21721 agccttgatg ctttttttgt gactttcttt actgtttcca tgccttcttt atggccagca
    21781 actcgtggca aacgacggtt ttgcgcccaa cgaccattgc cgtccatgat gatggcaatg
    21841 tgctgaggaa ttggtccatc ggtattaaat tgaaattcgc ttttttcttg aatatatttg
    21901 tttttttgcg gaaaaaaacg taacattctt tttcctccta tacttaaaag attcccacta
    21961 tcttaagtat tatagcaata tctaactgtt tttcctatga aatcattgtt tttttattta
    22021 aaaactgaat aattttttct ttttttaatt tttaaataaa agaaatataa ataaaaacag
    22081 aggaagcgga atgatccgcc cctctgtttt tatgatttta ttaaacttcc aataattctt
    22141 tttctttatc agcagcgatc ttatctactt ctttgatgct gttatctgtc aatgtttgta
    22201 cgtctttttc taatccgcgc aaatcatctt cggtaatatc accatttttt tgttgttttt
    22261 tatattcatc gatcgcatca cgacggatgt tacgaatcgc gatttttgca ttttctgctt
    22321 cttttttaac atctttagct aattcttttc tgcgttcttc tgtcagttgc gggataacta
    22381 agcgaatgac atttccatca ttggttggac tgattccgat atcacttgcc ataatagctt
    22441 tttcaacatc ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg
    22501 gaatttggat tgatgccatt tggttcaatg gtgttggcgc accgtagtag ttcacagtaa
    22561 tacggtccaa aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa
    22621 gattttgtgc tgcttttttc attttttctt ttgcttctgt cataattgca tctgccattt
    22681 ttatttcccc cttacagttg ttccgatatt ttcgcctaag attgcacggc gaatatttcc
    22741 gtgttcattt aaattgaaga caagcagtgg aatatcatta tccatgctta gtgaacttgc
    22801 agttgaatcc atgacttgta atcctttggc aataacttcc agatgagtca gttcttcaaa
    22861 ttttactgca tttgcatcta ctttcggatc agcagaataa acaccgtcta cattgttttt
    22921 agccatcagg ataacatctg cgccgatttc tgccgcgcgt aatgctgctg ttgtgtctgt
    22981 cgagaagtac gggttgccgg taccgcctgc aaagataacg atccgtcctt tttctaaatg
    23041 acgttctgct ttacggcgaa tatatggctc tgcgatttga cgcatttcaa tcgaagtttg
    23101 cacgcgtgtc ggtacaccca cgttttccaa cgtatcctgt aaagctaagg cattcataac
    23161 tgtagctaac atacccatgt aatcagcttg agcacgttcc atgcccattt gtgctccgat
    23221 ttgtccgcgc cagatatttc cgccgcccac aacgattgcc atttcaacgc ccagctcgtg
    23281 aacttctttg atttcttgga tgatttcttt gattactggt ggtttgattc caaatcctgc
    23341 gtccccagct aaagcttctc cactcaattt taagactaca cgctgatatt tcggtgtgac
    23401 catgttcatt cctccaatat tttctattgc cattttacca tacttagatg atttcgacac
    23461 tctaagactg gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc
    23521 gtctgttatt caacagaatt attttttcac ttggctcata acttcttcta caaagttatc
    23581 ttcgcgtttt tcgatacctt cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc
    23641 ttttgatgca acgtattttt caacagtcat atcaggatct ttaacgaatg gttggtccac
    23701 caaagcgatt tctgctttga atttcttcaa gcgtccttct accatttttt caacgatatt
    23761 cgctggtttg ccttcgttca atgcttgttc tgtaagaaca gttttttcat gttctaattc
    23821 agcttctgga atttgtgatt cgtttacata acgaggattg atagctgcta cgtgcatagc
    23881 tacgtctctt gcaactgttt cgtcagttgt tccatctaga acagtcaata cggcaatacg
    23941 tccgcccata tgcaagtatc caccaaatgc agcattgtcg tctttttcta caacttcaaa
    24001 gcgacggaag ctgatttttt ctccgataac ttgagttgct tcgatcaagt cagattcaat
    24061 tgtaccttta tctgttttga ttttcattgc agcttccatg tctgctggtt tgttttcagc
    24121 aacaagttct gcaatttctt ttactaaatc ttggaacatt tcgtttttag aaacaaagtc
    24181 tgtttctgag tttacttcaa cgattgctgc agtgttacct ttcacagcaa ctgaagccaa
    24241 tccttcagct gcaatgcggt catttttctt cgcagccttt gccattcctt tttcacgtaa
    24301 aagatcaact gctttttcca tgtcgccttc tacttcaact aatgcttttt tcgcatccat
    24361 cattcctacg ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat
    24421 gtttaactcc tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc
    24481 ttgtcgccca gcctttgaaa cagctccatc attcaaaatt attctgctga agaattgtcg
    24541 ccttcaacaa catctacgat ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct
    24601 tcttcaacta cttgatcttc accttggttt ccttcgatga aagcatcagc catttttgaa
    24661 gtaatcaatt taacggcacg aattgcatcg tcgtttgaag ggattactac gtcgatttca
    24721 tctggatcgc agtttgtatc aaccatagct acgattggga tatttaattt gtgtgcttct
    24781 tgaacagcaa tacgttcttt acgtgggtca acgatgtaca ttacatctgg gattcttggc
    24841 atatcagcga taccgcctaa gaatttttca agacgttcac gttctttgtt caaaccagca
    24901 acttcttttt taggtagaac ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc
    24961 aaacggctga tacgtttttg gatagtatcc cagtttgtca atgttccgcc taaccaacga
    25021 tggttaacga agtattgtcc tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt
    25081 ttttttgtac ctacgaataa tgctacgccg ccttcttcag caacgctctt catgtaatca
    25141 taagctgcat ctactaattt aactgttttt tgcaagtcaa tgatgtagat tccgtttctt
    25201 tctgtgaaga tatatttctt catttttgga ttccagcgac gagtttggtg accaaagtgt
    25261 acgccggctt ctagtaattg tttcatagaa attactgcca tgtttgtttc ctccgatttg
    25321 gttttatttt tcctcttctt atcttctacc ttgccggcaa actttttcaa gcacctaccg
    25381 acaatccaat aagaatgtgg atttgctgat gcataaagca ccctcagcta ttatacagac
    25441 aaaagatccg tatcgcaaga gcaaatatac ttttctgctc aacattcaat agtttacctg
    25501 aaagataaaa agaattcaag aaaaacagta gatttctctt tgtttcttta ttatcctttt
    25561 tccataaaat aattttaggt ataaaagacg aatattattg ggcaatcctt cttttttttg
    25621 ttatgataca tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat
    25681 ttgctgatta aacaagtcat tgaagaattc ccgatccgta cgcaagaaga actattaaac
    25741 aaacttgggc attatggcgt taccgccacg caggcaacga tttctcgaga tatccgtgat
    25801 ctaaaaatcg tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa
    25861 aaaggaactc ctgagactag gactgaggaa gaggatcggc tggttcaaat gattgaagac
    25921 attgtattga aagtcgaacg agtccacttt ttgacgatcg tcaatacctt gccagacaat
    25981 gctcatttat ttgcatctgt tttagatgag atcaaacctc cctctattgt cagtacaatt
    26041 gctggattcg atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa
    26101 gaatttcttc accatccaac tgaagtatca cttttttaat cagagcacgt attcctaata
    26161 tggaatgcgt gtttttttat tttcaatatt ttccttattg aataaacctt tacactcttt
    26221 acaaaaatac atcatttttt attcaaattt ttcaacacct tgattgaatc acaataatca
    26281 tcatttatat tggatgttaa caagagcagc ttcttgctct ttgtgtattt caaaaaagaa
    26341 atgaaaggaa atcatatgta taaattatta acttctggac cattgacgac ttctgaaaca
    26401 gtgaaaaatg ctatgctgat cgatcattgt acttgggatc aagaatacaa agaaattaca
    26461 caatggataa gaaaaaaatt attggatctt gctcaagtat cggaaggaat atacacaacg
    26521 gtcttaatgc aaggtagcag ctccttcggg gtagaatcgg tattaagttc tgtgatcagg
    26581 ccagaagata ctgtactgat ttgttcaaac ggcgcatata gacaacgaat gatcaacatg
    26641 tgcgaatgtc tgaaactgaa ctataccgtt tatgaggtat cggaacatca aatcctccaa
    26701 gctcatgtga ttacacagct gattgaagag aatccgtcta tctccgcact agcaatggtc
    26761 catagcgaga ctacttccgg tattttgaat aatttggaag agattgctgc tgtcacacaa
    26821 gaaagagatc ttttgtttat agtggacgcg atgtcaagct ttggtgggat accgatttgt
    26881 gtagccgaac tagggattga ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt
    26941 ccaggctttt cttttgtact tgctaataaa gcggaactgg aaaaacgaaa aaatgttagc
    27001 cgaagtttgt cacttgatct atatgaccag taccgcgtga tggaaaacga gaatggcaaa
    27061 tggcgtttca cttcccctac ccatacggtt ttggcttttc aacaagcgct gaaggaacta
    27121 gaggcagaag gaggaatcga aactcgatat tcacgttatc tcgccaataa tcatgtgcta
    27181 cgacaaaaaa tgacagagat ggggttccag cctttcattc aagaatatca ggggccattc
    27241 atcacctctt ttctttaccc taaggactgg cgatttgatt ttaatcattt ttatcagtat
    27301 ttaaagcaaa atggatttgt gatctatccc gggaaaatca gcgatcatga atgttttcgt
    27361 atcggaaata ttggcgagat ctatccggaa gatattttgg aactgacagc aattatcaaa
    27421 gactataagg agaacgttaa tgattaaaac tgtaattttt gattgggcag gaacaactgt
    27481 ggactttggc tgtatggctc ctgtccacgc ttttagaaat gcttttctag aaaaaggaat
    27541 ccaactgact gacaaagaaa tccgctaacc gatgggaaaa ttaaaatggg atcatatcca
    27601 acagcttctt tccctccctt ctgtcaaaaa acaatggata gctatttatg gacaactacc
    27661 tcaggaaaaa gaggtggagg aattgtatca acaatttgaa cattatctgt ttaaagattt
    27721 ggtcaagcat tctaccttga aacccgacac attggaaacg atcgaacggt tgagaaacgc
    27781 tgggatcaat attggttcca cgaatggatt taatgctcct atgatgacaa tcgtagcaga
    27841 aaccgcaaaa aaagaaaggt attcaccaga cttcatggca acttttgaag acgtcgaagg
    27901 gtatggtcga ccgtttcctt atatgatcca taaaaacatg cagtacttca aaaacaagtc
    27961 ggttgatgaa gtaatcaaag tcggagatac ggtctcagat atccaggaag ggaaaaatgc
    28021 cggagtgttg acagtaggtg taattgaagg tagttcgcta atggggattt cttatgatga
    28081 gtaccaaaaa ttagagatgg ggaaaaagat cgtttaaaac agtctgttgc tcttagattc
    28141 caacaagccg gcgctgatat ttgtgtaaac aatttaaaag agttaacaga atacattttg
    28201 tacgctgaca tgaatccaga atgactttta tacaaaataa gaggttgtga aaaagctgtc
    28261 ctgtatcaag taataaaaac agccaagcaa aaatagctcc tcatattttt cgtttggtta
    28321 ggcttattac cgcagatgca agcttttgaa caccgtttat tcgaaaaaaa aaagggagct
    28381 gtgacacgac ttttgtcaca gtccccttat ttgcaaacaa acagtgttta agagacaacc
    28441 tctccactaa acggtgttca ttcagctgac tctcggtatt tgctcaaaaa acgataaaat
    28501 ttgaaaagct attttctctt ttgtttctct aatactcgga tcagaacgct gatttcacat
    28561 tctctttttc ttacatctta tctggtgcat gaagtcctag caaacgaagg tcttctttca
    28621 ataatacagt cactgcataa actagagcta agcgtgcttc tttttgttca tcatcagcta
    28681 aaatcttcgt atgagcatag tatttgttga atgcttgtgc taatttgatt gcatgtttcg
    28741 caatcactga cggttcgtat ttttccccag cagacaagac cgtttcaggg tatttttgaa
    28801 ctaatttaac cacttcccag ctggtatcgt cattcaatgc atagtctgct gtttctgaag
    28861 ggacaaatcc tgctttttcc aacaggctga ctgcacgagc atgtgtatat tgcacataag
    28921 gtccagtttc tccttcaaat cgaacaactt cttccaggtt aaagtcaaat gtattcagtc
    28981 gatcgttttt caaatcgtgg aaaatgactg ccccaacccc aacttgttta gctacagcat
    29041 ctttgttttc caaatcaggg tttttctcgc tgatttgttc tttagcagat tggattgctt
    29101 catttaatac ttcttctaat aagacgatct ttcctttacg agttgataat tttttaccac
    29161 cttgcgtgat caaaccaaat ggaatatggt gcatgtcgtc agaccagtca aagcccattt
    29221 ccttcaagac agctttcaac tgtttaaaat gatagctttg ttcattccct actacatata
    29281 gagattgttt gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa
    29341 tgtatagtgt cgcaccgtct gacttcttaa tcaacgctgg attcaggtca taagcagata
    29401 ggtcaacgat ctctgcacct ttatcctcgt tcagcaaatg tttttcttct aaaagtttga
    29461 cgatttcatc catcttgtca ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt
    29521 ccagcagatc atagatttta ttgaattctt tcatcgattc atcacggaac cattgccata
    29581 attgaatagc ctctttatcg ccttcttcca gacgtttgaa ccaagcacga gcttcttcat
    29641 ttaattctgg ttctgtttca gctacttcat ggaattggac gtataaacga agcaattcat
    29701 tgattggttc tgctttaaca gcttcttctg taccccattt tttgtaagcg acgatcagtt
    29761 ttccaaactg tgttccccag tcacctaaat ggttgatgcg gattggctga taaccgattt
    29821 tttccataat gaaaccgatc gaattaccga taactgtcga acgcaaatgt cccattgaga
    29881 ttggtttggc aatattagga gaagacatgt cgattggtac tgttccttga ttcccgatgt
    29941 tgctatcccc ataatgttct ttttctttca ctactgtttg caaaactttt ttgctgataa
    30001 gttctttatt catgaaaaag ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg
    30061 cagaatcgat tttttctgct aaatctgcag cgatttgctg tggcgctttt cggtaaactt
    30121 ttgctaatga aaaggctgga aatgcaacat ctccgtgttc agctgatttt ggattttcta
    30181 gtaattgctc gacttgttcc agtgttaaat catctttgac agcgtcatag acagcttttg
    30241 cgacaagatc tttattattc atctttattc ctcctaaagt gttcccttga ttgttaaaca
    30301 aaaaaaatct ctagcgacgg catacttagt atgccatcac tagagacgag atttcccgcg
    30361 gtaccactcc aattgtccta aaaaaggacc agctccatct gatatcggta gacgaccgtc
    30421 tgtttaaaac agaattctcc aagtgcgctt cgttgtatct tcttcattcg gcttccacca
    30481 cttccgaatt cgctagccaa gaatgataca agtactctct tgttcaacaa atcttagtct
    30541 tattaaactt tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt ttcataaaaa
    30601 aacaatccag atggcctaaa ggagaagaaa taccacctgg atagctataa atcatttcgt
    30661 aaatacgaaa gacttaacaa attaatcagc tacgataatc gttccttctt cagaagctaa
    30721 aagattctcg atattttcta aagaagtgat gattgcctta gcgtttggtt ttgcttcaac
    30781 aaatgcaata gctgcttcta ctttcggaag catgcttcct ggagcgaatt gcttttcatc
    30841 aatgtattgt ttcatttctg aaacagtaac agtttctaat ttcttttgat ctggtttttg
    30901 gtaattgaca tacacattgt ctactcctgt caatacgatc agcaaatccg catcgatgat
    30961 ttcggctaat ttttctgaag caaaatcttt atcgattacg gcttctcgtc cttctaatcc
    31021 agtagctgtt tctacaacag gaatcccacc gccaccaaca gagactgtga taactccttg
    31081 atcgaccagc gtttcgatga cacgtgcttc tttaatcgag atgggtttag gggaagcgac
    31141 tacttttctc catccacgtc cagcatcttc tttgaatgtc acagtggagt cagcattcat
    31201 ttgttctttg gcttcttctt ccgtgtagaa tggaccgact ggtttacttg gatttttgaa
    31261 tgaaggatca ttttcatcca caataacttg agtgactaaa gaaaccacat ctttatcgat
    31321 ccctttttcc ttcagtactt cgcccatggc gttttgtaac cagtaaccga tagatccttc
    31381 tgtcattgca acacatgtat ctaaaggcat tgccggtgtt ttctcagaat cagcagcttg
    31441 ctgctgaatc aataggttac cgacttgcgg accatttccg tgggagatga tcaattcatc
    31501 tccttgttcg ataaatttca ctaaatactt tgctgtttcc attaacgctt cttgttgggc
    31561 ttttgcactt gcatccgtag acaagatggc attgccccct aaagcgacaa ctacttttcg
    31621 tttggccatt tcattcgtcc tttcttcagt tctaccgaat tttctagata acaaaacgag
    31681 ccagcgtttt gtgattttcc ctcaatgctg actcgttttt tcgtgagatg aatgtggtta
    31741 tgcttaaacg cgagggataa acaagttacc taatgttgct gccatgatcg ctttgattga
    31801 gtgcatacga ttttctgctt ggtcaaattg acgagcatat ttgctgcgga agacttcatc
    31861 tgttacttcc atttcagtga tgccaaagcg ttctttcatt tgttcaccgt aaacagtgtt
    31921 tgtatcatgg aaagctggca agcagtgcaa gaagatcaaa cgatctgtgt tgtgtgtttt
    31981 ttctaccatt tccatattga tttggtaagg tttcaacaat ttgatacgtt cttcgaattt
    32041 atcttcttca cccattgata cccatacatc agaataaagg acgtctgctc cgtctacacc
    32101 tttatcaaca tcgtcggtaa tcatcaattg tgcaccggat ttttcagcaa agccttctgc
    32161 cattttgacg atttcttctt caggttgtaa ttcttttggt gcaacgatac gcatattcgc
    32221 tcctaaaatt gcacctgtga ctaataaaga gttagccata ttgtttcgac catctccgca
    32281 gtaagcaaca gtgatacctt ctactgttcc aaagttttct tggatagtta aaaagtcagc
    32341 gatcatttgt gttggatgcc attcatcagt caagccattc caaactggaa caccagagaa
    32401 ttctgctagt tcctctacca ttttttgact gaatccacgg aattcgattc cgtcaaacat
    32461 acgtcctagt actttagctg tatcttctgt ggattctttt ttacctaatt ggatatcatt
    32521 tgcacctaga tattcaggat gtgcacctag atcgatcgct gcagtagtaa atgcagaacg
    32581 agtacgtgta gatgtttttt caaataaaag cgcaatattt tttccttcta gatagtgatg
    32641 tgggatacca cgttttttca agtctttcaa gtgttctgaa aaatcgatca aatattgtaa
    32701 ttcttctttt gtaaagtctt tttctgctaa taagcttctt ccttggaata ctgattcttt
    32761 catattaaac attctccttt agattgtttt tatttaagat taaagaggaa atcccgacgg
    32821 cagctgacag taagactgcc gtcaggaaac aaattacttc aagtcttcac ggacaagtgg
    32881 ttggctcatg cagcgtggac caccacgacc tcgtgacaat tcacttgaat tgatttctaa
    32941 cactttgatg ccatagctac gaagcaattc gttcgataca taatttcggt tataagtgac
    33001 tacaacacct ggagcgattg ccaatgtgtt tgatccatca ttccattgtt ctcgaggtgc
    33061 aacgatctca tctccattac cagttgggat cagtacaaga tcatctaatc caagagcagc
    33121 tttcatcgtt tcgtgtaagt tatctgaatg agtaatcttg atatcatcac catctggttc
    33181 gattgtaaag acatccatct taccgtcttt actttgaatt gctggatgga tagtgaattt
    33241 atcgtgatct accattgtaa atacggtatc caaatgcatc attgcacggt tgttcggaat
    33301 tttgattgcc aacacttttt caaatcctga attttttgcg aataggtggc gtgctaattt
    33361 ttctaaagct ttcgcattag ttcgttgaga aatcccaaca gctactactt tatcgcttaa
    33421 gatcaattcg tctccgcctt cgatatgatc tggattttca cgatccaacc atacttcgat
    33481 tcctttattt gcaaatcttg gatgatgttt caaaatatat tctgtaaaca tcgattcacg
    33541 acgacgtgct tcaaaagtca tcttattgac tgtcatccca tttcccattg atgcggaagg
    33601 gtcgcgtgta aagtacaagt ttggcattgg gtccatgtaa aatggatagt catcgtctgc
    33661 tgacagatca tatagactgc tagaacgtac ttcgatatct tttgtacgaa ccccagccat
    33721 gattttatct accatttctt gcgtttccat tgatagaagg aactcatgaa gaccctctct
    33781 tacagcattc gatgcaatat gagattcatc caacatttta ttcaagaatt gttctttgac
    33841 attgcctgcg tcaatcgctt ctgccgctaa tttttccaag taaagagtct cgacgccttc
    33901 attttgcaat gtcttcgcaa agttgtcatg ttcttcttga gcaatcggaa gatatggaat
    33961 atcatcgaac aacagacgat ccataatatc tggtgtcaaa ttttccactt cttgtccagg
    34021 ccgtttcagt aaaactgtct ttaatttccc tatttcagag aaaacgtgaa taggtttatc
    34081 catgttgtgt tgcctccttc tttttgatta catgtatagg atatcctcct tttgtaatca
    34141 ctttcataat tttgtctcat tcaaaatgag acattcctca cattaatatt cgataattaa
    34201 ataattaaac gtttttctat ttttaagcgt aatttattca ttttttattc tgtatacact
    34261 ttcaaaaaaa tatttattca ttgaaaaaat ttttttgaat aaatta
//
LOCUS       DAYYKN010000012        33713 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000012 DAYYKN010000000
VERSION     DAYYKN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.012"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACHBB7_000503"
     CDS             <1..1152
                     /locus_tag="ACHBB7_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFM9208481.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHBB7_000504"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHBB7_000504"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM9208482.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACHBB7_000505"
     CDS             2728..3003
                     /locus_tag="ACHBB7_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM9208483.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACHBB7_000506"
     CDS             3009..3776
                     /locus_tag="ACHBB7_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM9208484.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACHBB7_000507"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACHBB7_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM9208485.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7793
                     /gene="recQ"
                     /locus_tag="ACHBB7_000508"
                     /pseudo
     CDS             6034..7793
                     /gene="recQ"
                     /locus_tag="ACHBB7_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
     gene            7907..8209
                     /locus_tag="ACHBB7_000509"
     CDS             7907..8209
                     /locus_tag="ACHBB7_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM9208486.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8212..9375
                     /locus_tag="ACHBB7_000510"
     CDS             8212..9375
                     /locus_tag="ACHBB7_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9208487.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9401..12829
                     /locus_tag="ACHBB7_000511"
     CDS             9401..12829
                     /locus_tag="ACHBB7_000511"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM9208488.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12850..13974
                     /locus_tag="ACHBB7_000512"
     CDS             12850..13974
                     /locus_tag="ACHBB7_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM9208489.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13955..14401
                     /locus_tag="ACHBB7_000513"
     CDS             13955..14401
                     /locus_tag="ACHBB7_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9208490.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14385..14723
                     /locus_tag="ACHBB7_000514"
     CDS             14385..14723
                     /locus_tag="ACHBB7_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM9208491.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14842..15402
                     /gene="rsmD"
                     /locus_tag="ACHBB7_000515"
     CDS             14842..15402
                     /gene="rsmD"
                     /locus_tag="ACHBB7_000515"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM9208492.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15399..15890
                     /gene="coaD"
                     /locus_tag="ACHBB7_000516"
     CDS             15399..15890
                     /gene="coaD"
                     /locus_tag="ACHBB7_000516"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM9208493.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15880..16923
                     /locus_tag="ACHBB7_000517"
     CDS             15880..16923
                     /locus_tag="ACHBB7_000517"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM9208494.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17082..17810
                     /locus_tag="ACHBB7_000518"
     CDS             17082..17810
                     /locus_tag="ACHBB7_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM9208495.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17926..18171
                     /locus_tag="ACHBB7_000519"
     CDS             17926..18171
                     /locus_tag="ACHBB7_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9208496.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18168..18386
                     /locus_tag="ACHBB7_000520"
     CDS             18168..18386
                     /locus_tag="ACHBB7_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM9208497.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18522..19061
                     /locus_tag="ACHBB7_000521"
     CDS             18522..19061
                     /locus_tag="ACHBB7_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM9208498.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19054..21327
                     /locus_tag="ACHBB7_000522"
     CDS             19054..21327
                     /locus_tag="ACHBB7_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM9208499.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21365..22411
                     /gene="holA"
                     /locus_tag="ACHBB7_000523"
     CDS             21365..22411
                     /gene="holA"
                     /locus_tag="ACHBB7_000523"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM9208500.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22500..22754)
                     /gene="rpsT"
                     /locus_tag="ACHBB7_000524"
     CDS             complement(22500..22754)
                     /gene="rpsT"
                     /locus_tag="ACHBB7_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM9208501.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23154..23423)
                     /locus_tag="ACHBB7_000525"
     CDS             complement(23154..23423)
                     /locus_tag="ACHBB7_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208502.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23685..23813)
                     /locus_tag="ACHBB7_000526"
     CDS             complement(23685..23813)
                     /locus_tag="ACHBB7_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208503.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23893..24348)
                     /locus_tag="ACHBB7_000527"
     CDS             complement(23893..24348)
                     /locus_tag="ACHBB7_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM9208504.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24486..26414)
                     /locus_tag="ACHBB7_000528"
     CDS             complement(24486..26414)
                     /locus_tag="ACHBB7_000528"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9208505.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26611..27444)
                     /locus_tag="ACHBB7_000529"
     CDS             complement(26611..27444)
                     /locus_tag="ACHBB7_000529"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM9208506.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27636..28340)
                     /locus_tag="ACHBB7_000530"
     CDS             complement(27636..28340)
                     /locus_tag="ACHBB7_000530"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9208507.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28363..28989)
                     /locus_tag="ACHBB7_000531"
     CDS             complement(28363..28989)
                     /locus_tag="ACHBB7_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9208508.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29315..31690
                     /locus_tag="ACHBB7_000532"
     CDS             29315..31690
                     /locus_tag="ACHBB7_000532"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM9208509.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31714..32205)
                     /locus_tag="ACHBB7_000533"
     CDS             complement(31714..32205)
                     /locus_tag="ACHBB7_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM9208510.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32392..33312
                     /locus_tag="ACHBB7_000534"
     CDS             32392..33312
                     /locus_tag="ACHBB7_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9208511.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33466..33594
                     /locus_tag="ACHBB7_000535"
                     /pseudo
     CDS             33466..33594
                     /locus_tag="ACHBB7_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
BASE COUNT        11158 a         5900 c         6989 g         9666 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt atccagcagt cccaaagaga agaagggcaa aagcaaaaag
     7081 aatatgaaaa attaaaagcg atgacagaat acgtgtatat tgagtcctgt cttcagcaat
     7141 acatcctaaa ctattttggc gagacaagca gtccgtgcaa ccgttgtggt aactgtttag
     7201 atgacagaga aatagtagaa gtaacgacag aagcacagat ggttctttct tgcttaaaac
     7261 gtatgggtga gaactatggt aaacaaatgc tgatgaaagt cttagcagga tcgaaagaac
     7321 aaaagctgca agctttaggt tttggacatt tatctaccta tggactgatg aaaaaccaat
     7381 cgcaaaaaga aacgatgcag ttaattgaat atttgatatc gaatggctat cttctgacca
     7441 tcaatggtga gtatcctgtt ttgaaagtaa cagaaagagg gatacaagta ttgaaaggac
     7501 aagaatctgt ttatcgaaag gaacccaaga aagtccagca gctatcggat gaagaaaccg
     7561 atacactatt tgaagtgctg cgggaactgc gaacggatct agcatcagaa gcaggcgttc
     7621 ccccgtatgt cgtattttca gattccacat tgaaagaaat gagccgaata cgtccatcaa
     7681 gcagattgga gatgcttcag ataaaaggag ttggccaaag caagcttgat aaatatggcg
     7741 aagccttttt atctcggata aaaaatgcgg actctaacgt tctggataag tagaaagaac
     7801 gaatacgtaa aaatgaataa aaacaactgg ttaaatctgt tttttttgct ccttatgaaa
     7861 atcaaatatg ttataattaa ggaattagaa tgggaggtaa gcacagatgg attctttgtc
     7921 gaaaaaactt gcgatccaag tattaaatga tgaagcagaa cggatcaaac gattaatccg
     7981 aaatcaaaac aacagtctct gtatctcaca atgcaaagcc ttcgaagaag tggtagatac
     8041 acagatgtac ggcttttccc ggcaagtcgg ttttgctgtc cgtataggag taatcggaaa
     8101 gaacgagggc cagctgatgt taagtgagct agaacgagaa ttgaatcagt tatatagtga
     8161 ggtttaccaa gaaatttatg ataaaaaaga agttggcaag gaggaataga cttgcctaag
     8221 aaagtgaaaa aaaggcatct attagactat agcatcttga ttccctattt aattttatgt
     8281 gtattgggat tgatcatggt atatagttct acgtcgtatc tattattaga aaacggacaa
     8341 aatccgtccg catcagtcat taaccagagt attttttggg tgcttagctt gattgctatc
     8401 gcactacttt ataaaatgaa aacagatgtt ttgaaaaacc agcgtttgat catggcagcc
     8461 atcgcagttt tgacgatttt gctgctgatt gttgtgtttt ttggtaagga gatcaatggg
     8521 gcgaaaggtt ggctgcaaat tgcaggattc tcgatccaac ctgccgaata cctaaaaatc
     8581 atttctatct ggtacctgtc tctgacttta tctaaaagac aaaacagtgt acaaaaagat
     8641 tttcttggga ctgtgaaacg tccacttgca atggttatcg gacttactgc attagtcgct
     8701 attcttcccg attttggtaa tgcggcagtt atctttttga ttattttggt tctgctttta
     8761 gccagcggag tcaattatgt gtatactttg attgtaggag taggaggctt ttgtctaagt
     8821 acttttacga tctggctgat caatatcaca aacggaaaga tcttacccgg acgtttacag
     8881 tatatctata atcgatttgc tatttaccaa aatcctttct cagacgaatt aaataaaggg
     8941 catcaactag tcaacggcta ctatgccatg ttcaacggag gactatttgg caggggatta
     9001 ggtaacagta tccagaaaaa aggattttta caagaagcac aaaccgattt tatttatgca
     9061 atcgtagtgg aagaactggg agtgattatg gggattttga tattagcgtt gctattcttt
     9121 atgattgtgc gcattgtcct tgtaggtatc cgttcaaaag atccgttcaa ttcgcttttg
     9181 tgcattggga tcggcgcaat gtttttgatc caagtatttg tcaatcttgg aggaattact
     9241 ggcgttattc ctttgacagg gatcacgttc ccatttttaa gtcaaggagg ctccagtttg
     9301 ctgatgcttt cgatttgtgt cggctttgtc ctcaacatca gtgcggatga aaaacgcaag
     9361 agcttaggtt tataataaaa aaagatgtag gagtggttcg atgaaaaaag ttttagttgc
     9421 taacaggggt gaaatcgctg tacgtgtatt tcgtgcttgt acagaattag gaattaaaac
     9481 tgttggaatc tatgcagaag aagatgaata ttccgtacac cgtttcaaag cggatgaagc
     9541 atatttagta ggacaaggaa aaaaaccgat tgatgcgtat ctagatattg aagggatcat
     9601 ttctatcgcg aaagaatgcg gagctgacgc tattcatccg ggttacggtc ttttgtcgga
     9661 aaacttgaat tttgcgcaac ggtgcgaaga agaaggcatc atttttgttg gacccaaact
     9721 gcatcattta gatatttttg gagataaaat caaagcaaaa gctgcagcta tcgaagcggg
     9781 gattgcttcg attccaggca cagatggacc aatcgcttct atagacgatg ccttggagtt
     9841 tgcgaaacaa tacggttatc cgattatgat caaggcagct ctaggcggcg gcggacgcgg
     9901 catgcgtgta gctcacgatg aaaagagtgc aagagagggc tacgaacgtg cgaaaagcga
     9961 agcaaaagca gcctttggta gtgatgaagt gtatgttgaa aaatacatcg ctaatcctaa
    10021 gcatatcgaa gtacagattc tcggggatac gcatggaaac gtcatccatc tttttgaaag
    10081 agattgttct gttcagcgcc gtcaccaaaa agtagtagaa gtagcgcctt gtgtctctat
    10141 gaatgaacag caacgtcaaa aaatctgcca ggcagctgtc caattgatga aatatgtcgg
    10201 gtatgtcaac gcaggtacag tagaatttct tgttgaagga gatgattttt actttatcga
    10261 agtcaaccct cgcgtccaag tggaacatac gatcacagaa atgattacag atattgatat
    10321 cgtaacaacg caactgttga ttgctcaagg tttggatctg cataaagaaa tcggattgcc
    10381 tcagcaagaa ggaattaaat taaatggttc agccattcaa tgccgaatca ctacagaaga
    10441 cccgttgaat aactttttac cagacacagg gaaaatcgat acgtatcgtt cacctggtgg
    10501 ttttggcgta cgtctggatg tcggaaatgc ttacgcgggc tatgtagtga caccgtattt
    10561 tgattcattg ctagtaaaag tatgtacgca tggtgctacc tttgaaacag ccattcagaa
    10621 aatggaacgt tgcttgaaag aatttagaat ccgtggagtg aaaacaaaca ttccatttat
    10681 gctcaatgtc atcacacatc ctgaattcca atcaggaaat gcaaaaacaa ccttcattga
    10741 tagtacagca acattatttg aatttcctcg actacgagac cgcggaaaca aaacgatgaa
    10801 atatatcggt gaaattactg tgaatggttt tccaggtatc gaaagtggcg aaaaaccatt
    10861 ttatgaagaa ccacgtatgc ctaaagatct aatcacaaga actgattacg taacggctaa
    10921 gaatgtatta gatgcgaagg gtgccgatgc tttggtcgaa tggatcaaag gacaggaaaa
    10981 tttgttgttg acggatacga catttagaga cgcccatcaa agtctgctag caactcgtgt
    11041 tcggacaaag gatttcaagc agattgcacg tttgactgga gaaggtcttc ctgaactctt
    11101 ttcaagtgag atgtgggggg gagcaacatt tgatgtggct taccgctttt tgaatgaaga
    11161 tccttggcaa cggttgagaa aaattcgttc tttaatgcct aatacgttgc tgcaaatgct
    11221 tttcagaggt tcaaatgccg ttgggtattc taattatcct gacaatgtat tggtggaatt
    11281 tgtcaaagaa gcagcagcgc aaggaatcga tgtatttcga atctttgata gtttgaactg
    11341 gactccacaa atggaaaaaa gtatccaagc tgtacgggat actggtaaaa tcgcagaggc
    11401 agctatttgc tatactggcg atatcaacga tccaagtcgg gcaaaataca atgttcagta
    11461 ttataaagac atggcaaagg aactagaaca actgggtgcg catatcattg caatcaaaga
    11521 tatggctggg ctgcttaaac cgcaggcagc ttatcgtttg attagtgaat tgaaagaaac
    11581 gacagatctg ccaatccatc tgcatactca tgataccagc gggaatggaa tcatcactta
    11641 ttctgctgct tcaaaagcag gagtagacat cgttgacgtg gcaatgagtg caatgagtgg
    11701 gaatactagt cagcctagca tgagcagctt gtattatgca ctggtcaatg gtccacggct
    11761 gccagaaatc acgattgaaa atgcacagaa attaaaccat tattgggaag atgtgcgcat
    11821 gtattacaaa ccgtttgaaa acggcttgaa tgctcctgaa acagaagtat atatgcatga
    11881 aatgccagga ggacagtatt cgaatctaca gcaacaggca aaagctgtcg gattaggtca
    11941 tcgctgggat gaaatcaaac aaatgtatca tactgtcaac ttgatgtttg gcgatatcgt
    12001 taaagtaact ccatcatcta aagtagttgg agatatggca ctatttatgg tccaaaatga
    12061 tcttacagaa gaagatattt atgaaaaagg cgaaacacta agtttccctg aatctgttgt
    12121 gactttcttc caaggagaac tagggcaacc cgttggcgga tttcctaaaa aattgcaaaa
    12181 gattattctt aaaggcagac ctgcattgaa tgaacgtcca ggtcttttgg ctgaatcagt
    12241 cgacttcaac gaagtaaaaa aagaattggc agaaaaaatt ggctatgaac caaaacaaga
    12301 cgaagtatta agctatttga tgtatccgca agtctttttg gactatcaaa aagcatataa
    12361 tcaatttggc gatgttacat tactggacac accaacattt ttccaaggta tacgtttggg
    12421 tgaaacaatc aatgtacaaa tcgaacgcgg aaaaacgctt atcattcgtt tagatgaaat
    12481 cggagaaccc gatatcgaag ggaatcgcgt actgttcttc aatttgaatg gacaaagacg
    12541 tgaaatcact atcaaagata attcgatcat cagtacggtc caaacccgat tgaaagctga
    12601 accgacaaac cgagaacaaa ttggagcaac gatgtctggt tctgttttgg atgtattagt
    12661 gaaaaaaggc gataacgtta aaaaaggtga tacgttgatg attactgaag cgatgaaaat
    12721 ggaaacggcc atcgaagcac gttttgacgg agaagtcgca catgtatatg tgtcttcagg
    12781 agacactatt tcgtctggtg accttttgat tgaagtgact gagaaataat aagcgaaagg
    12841 tggggaaaca tgaaaagact gggtttgttt tggctgattc tgctattagt tattgtggca
    12901 ttctacatgg aaccagtctt gtttccgcca aaacttgaag tgaaggaaaa ctctgatcaa
    12961 caaatatcaa accaaatgac aacgtggaaa taccaggagc ttaaagcaac tggtttttct
    13021 acttatatcg gaaaaccgat cgatcagctg gaaaaggatt ttggccaagc ctatgatcga
    13081 ttaactagcg gatttggttt tgaaacaaga tattatgaaa ataaagctag tcatctcaca
    13141 tttgaagcga acatccaaga cagcagagtc acaactgtta aagtactgca aacagggcct
    13201 gctgatatcg cgccgtttta tttagggatg acgatgcaag atcttacgaa aatcactact
    13261 atctatacta atttcacttt tgagtaccat gatacagaag ttggaatcga actaatggaa
    13321 gaggatatga attatcgtcc gctaattgct tttgataacg acacttttgc tattttattt
    13381 tttgatcaaa caacaaacgg cctattttca gttgtgtatt tagataaaga tagtcttttg
    13441 aaactattgc catatcaagt gtttggtgaa ggccttccac attatcaagc agataaatcg
    13501 gctgattggg aatcaatcaa tcgcgttaaa gaacgaaaaa gtacgacttt attaaatatg
    13561 cttagaagag aagacgaact tcccatatac aatcaaacac ttgcctttca aaaccacagt
    13621 cagctgctac tacatgattt tttgacaagg cctgaggaaa tattgtcaga ggaacgtatg
    13681 gctgattggc aaaaatcgct aggcagtgct caaacgacag tgaaattctc actgacgaat
    13741 gatgaattgg agaagttaat cagtaaacaa aaattagaaa atactagtgg gctatttatt
    13801 catccggcga ttgacgctac tttttcgttt ttatattggt acagcgatcc ttacaaccat
    13861 gaccgtttca tggacccggg aacggattct gtaggcattg ctttttctaa agaaaatatg
    13921 ttagtcttat tgcaagaaga aaacggagaa agtagtgata gcagtgatcg ttaacgaaga
    13981 gctgtttcaa ctggaggatc aatgcctcca agtcgctgaa atgataaaaa agagtaaaac
    14041 gatggcttct tatcttgaac ataaacagtc aatggaagaa tctgcaaaag tttgccagtt
    14101 gaaagaagcg ttcttgtcta agaaagaagc atttgaaaat gtagcggcat atggtgagta
    14161 tgcacctgat tatcgtgaaa aaaaacgagc tgttcgaaaa gcaaaaagac agcttgatat
    14221 ggatgagcat gttgcagctt tccgatatag tgaaacacag ctgcaaagtc tgttggatca
    14281 aattgtccaa gtacttgccg aagaagtttc taatgaaatc aaagtagatg caggtagccc
    14341 gttttttgaa acgaaaaaac actttggatg cggaggtaat tgtcatgcaa ttagacaata
    14401 gtgaatttga aataacaaaa cgacgcgggc tgatcgtatg ggtctacagc ttaaaacaat
    14461 taaagaatct gaaaaagttc ggcttgttgc attatgtctc aagaagaatg aaatatgtcg
    14521 tgctttactt aaatgaagag acattggaag aaacagaaga acgaatccgt aaattacatt
    14581 ttgttcgaaa cgtagaacgt tcttaccgac cggatatcga aatgaatttt gccgaaaaaa
    14641 taggaaacaa aacagtatta aaagacgaag gtttcgaaat tgaagagcta aatacagaaa
    14701 tccgattagc tgaacatctt taaaaatctc ttatgagcag tttttaaata aaaaataact
    14761 tggataacga tcgatttttg atcattgtcc aagtattttt ttggtatact agtaccagta
    14821 ccatttctaa ggagtgagta gatgcggata atatcaggaa attatggcgg gcgccgttta
    14881 aaaagtttag caggggcgaa caccagacca actacagata aagtaaaaga atccatattc
    14941 aatatgattg gaccatattt tgatggagag acagtattgg atctgtttgc aggaagcggt
    15001 ggtttagcta tcgaagcagt atctagaggc tgcagtcatg cggtatgcgt agacaaaaat
    15061 taccaagcgc taaaaatcat caaagaaaac atcgaaatta cgaaagaacc agagaaattt
    15121 accacaataa aacttgatgc ggacaaagca atcgaacggc tagcagcgga gaaacaacaa
    15181 ttcgactact tgtttcttga tccgccatat gcaaaacaaa aaataatcga tcaaatccat
    15241 aaaatggaag aactgggact tttttctaaa accgcagtta tcgtatgcga aacggataaa
    15301 acagtcgaat tgccagaaac tgtggctgga ttccatcaaa taagaaaaca gatttatggc
    15361 ataacggccg taactatcta tagaaaagag ggaaatcaat gaaacgtgca ttattcccag
    15421 gaagttttga tccttttaca aaaggtcact tagatacggt ggaacgtgcg gctaaattat
    15481 tcgatgaggt agtaattggt gtttttatca atacttcaaa gaaaagtttg tttcccccag
    15541 aagaaagaat gacattgatc acaaaagctg tttctcatct tcctaatgta aaagtcatgc
    15601 atcaagaaaa tcaattgacg gtagaaacag caaaagagat tggtgcagat gcgttgatca
    15661 gaggcatacg aagtatcaaa gactttgaat atgaacgaga gattgctcag atgaatcatc
    15721 atctatatga tgaattggaa actgtcttct tattagcaaa accagaatat agtcacgtga
    15781 gttccagtat tttaaaagaa gtactgcact ttggcggaga tgtgagcagc tacttaccgc
    15841 cagtgatcaa cgaagcttta gctggaaagc gtgaatcaaa tgaaagctaa acaatggttg
    15901 aaaacagtac tgattatcat atttggaatg ttgatcattg gatttgtagt agttcccttg
    15961 ccgtattatg tagaatcacc gggagccacg atcgatctga aagaactaat tacggtcaat
    16021 gataaggaag ataaaggttc tgggtctttc tctcttacct ctgttggtat caggcaagct
    16081 acaggtttct tagctttaaa agctaaaatg accccttttg aagaattgat cagtgaagaa
    16141 gaattgactg gtggggcgac aaatgaagaa tataatcaaa tgcaacagta ttatatggat
    16201 tcttcccaaa atgcagcaat cgaacaagcg ttaaaacttg cagacaagcc ttataaaatg
    16261 gaattcaaag gcgtatatgt catgagtgta gagaaaacat cgaatttctt tggcaagctt
    16321 tcagttggag atacagtaac aaaagtagac gggaaaagct ttcaaagtac agaagcattt
    16381 atggattatg taaaaagcca aaaagttgga caaacaatcg aaatcagtta tttgcataat
    16441 ggtgaagaaa aaacagcttc tggtgatctg atcgagttac caacagataa aaaggcagga
    16501 ataggtatca cgcttactga ccataccgag atcgaatcag atacagatgt tcgtattaat
    16561 tctggctcta tcggtgggcc atcagcggga ttgatgttca ctttagaaat ctatgaacaa
    16621 gtcactggta aaaacttgcg acatggtaag caaattgcag gaactggaac gatcaacagt
    16681 gaaggagaag ttggccgaat cggaggaatc gataaaaaag tggcaagtgc ggataaagct
    16741 ggcgtagaga ttttctttgc accagatgat acgatttcag acgatgttaa gaaagaatat
    16801 cctgaaataa aaaccaacta cgaagaagca aaagctgccg ctaaaaaatt gaattcctca
    16861 atgaaaatcg taccagtaaa aacagtacaa gaagcgcttg actatctaga gaaaatgaaa
    16921 tgaaactaaa taattaaaga ggggttgtga cacgagttct gtcacaaccc ctctttttcg
    16981 tcaaaaatct tcacgataaa aataaaaaca aaagaaaaaa ttgacgtaat tagatgttga
    17041 agggacgatt ttgccagagc tcactgtttc ttcaatttta aatgaaagga gtaatgaatg
    17101 atttttactg gtcattcttt gaaaaaagaa caatgataga aaaaatcagc aagaaaatac
    17161 tagcttcacc caaattgatc atttggatgt ctatgatagt tcttatcttt tgtatcgcaa
    17221 gtggtttttt attatcaaat ttgctatttt atagagaaac agaagagact gctgcaaaag
    17281 aaatactttc ttcgtttagt tccagtggag aagatccatc agctgaagga aacgatgtag
    17341 aagaggatgc tcgtccaaag gtcatgtaca ccgatatcaa aggaagtgtg aaggagcctg
    17401 gtatctattc cttttcttca gaagaacggg tctatgatgt actaaagcgt gcaggtggat
    17461 tattagaaga agcggatagc gatcgaatca acttttctgc gaagatagaa gatcagcaag
    17521 tcctatatat ccctgctgta ggtgaagagc tgccagaaca tcttgatcag ttggcatcct
    17581 cggaaggaaa acagcctaca gcagattccg agcctcccaa aataaatatc aatacagcca
    17641 ctccatcaga acttcagcag attcctggga tcggatcagt caaagcgcaa gaaatcattc
    17701 gatttagaga agaaaacgga tccttccaga aagtggaaga cctgcaggag atttctggta
    17761 tcggtgaaaa gacagttgaa aaactaaaaa acttcgttac aattaaatag aatgattcaa
    17821 aatcgactgg cacgagcaat aaatggacgc taatggatac taagaataat ccaattctaa
    17881 gagcttggca ggacgaggag gtagtatttt tggataggaa atagcttgat tgttcgcaaa
    17941 gaaacattaa aaaaaccaat gctgaatgtc tatctccaaa ataaaatatc tgggattcat
    18001 attatgaata cggctgtcag cggcaataac agtcaagctc tacgagaacg ctttgccaaa
    18061 gatgtactta gctatacagc agataaagta tttattctca ttgggacgaa tgatctagca
    18121 gagcataaac aactttctaa agaaacgtac caaaaaatct gcagtggatg atagttcagc
    18181 tgaaaaaaga ctatagttct gatcatgctt tctttatcac gccaccagca gtcgatgaga
    18241 caaaacaaaa atatcggaat aacaagttga tcaaagaata tatctctgtt ctcacagaag
    18301 tagcaaaacg ccatgattgt gaagtatcga tttttatacg gaatttcttg aacaaatgga
    18361 ttcgaagaat ttttataatg ggctaatgga tgacggactt catttcggca gtgaagggta
    18421 ggatcttttg tcagacagga tgattcgtta tatttaaaga atattttgtt taagcaatga
    18481 gtacttttta atcaacttga aaaaacggag gaatataaaa catgttagaa aaatcttcaa
    18541 aagcatgcga tacgaataaa gaaaaagtta ctgataagtc acataatgaa cgcattcctt
    18601 gggaccagta ctttatggct caggcagtac tgttatcttt acgtagtacg tgtacacgtt
    18661 tagaagtagg agcgacactc gttcgagaaa aaaggatcat tgctggcggc tataatggag
    18721 cagtcagtgg agatgtccac tgcatagatg aagggtgtta cgtcgtggat ggacactgct
    18781 tgcgaacgat ccatgcagag atgaatgcac tcctacagtg tgcaaaactt ggtatcccga
    18841 cggaaggttc agaaatctat gtcacacatt ttccatgttt ggcctgtaca aaggctttgt
    18901 tgcaagctgg tgtgaaaggg atcaattatc tccatgacta tcgaaatgat ccttatgccc
    18961 aagcgttgat cgaacaatta ggtgtatctg tacaccaagt tgctttagac tcacagcatt
    19021 tttcaaaact tcaaaatgaa ttaacaggaa aacatgtcta gcgttttaga aaggcatgtt
    19081 cgaaatcggt tgatttttcc ggcaattacg tcgatgatta tctgtttgtt gtattacact
    19141 gacaattggg gttatcgttt gcttcttgcc ggaatttttt tatggttttc gctaaaagaa
    19201 ggacgtttta tcgcgtactt gtgtattttg tgtagcataa cggtatccat ttcttatagg
    19261 ctacaagagt attcattttt agaggaaaaa acaacatctg cgcaaattac tgtatttaca
    19321 gatacgattc gtgcaaatgg taattttttg acaatggaag gaaggttatc agagaataaa
    19381 caaaagctcc agctttctta tcaagtaaac acagaagcag aattgaaaac atggcttaac
    19441 ttccgaggaa agggaataga attgactgct gaaggcgagt tcatctctcc agcaaaaaag
    19501 cggaatcttt ataattttga ccaagaagct tacttcagga accatagaat ttctggacgg
    19561 ttccaaattc aaaagattga acagatcacc atgcaaaatc actggcgaaa ctggcttcag
    19621 agaaaaagag gatcttttat ctcctttgtc caatctgaat ttccgcaaaa aacaagtgta
    19681 tatattaatt ctttattatg gggctataaa gatgcaggat tcagagaacc agaagaaatt
    19741 tttagggaga gtggtttgct gcatctgttc agtctatcgg gtctacacat ccaattttat
    19801 ttagggtggc tgtattattt gtttcgacgt atcggatggc cactacacgt cagtattatt
    19861 ccgttgagtc tactaactat ctgttatgtt tctattgcag gaagttctat ttctgttttg
    19921 cgtgctagtt tgctgttttt attgcgattg ctagtcaaat tgctgaataa gcattattcc
    19981 accatggact tatttgcagt tgtcttttgg cttcttttac tatttgatcc tcgatttctt
    20041 tttcatgcgg gtggacagct tagttttttc atgagttttt tgtttatcct gatttctttt
    20101 gatggagatt ggttgaaaaa aacggcctct cacgtcctgt ttactatgat cacgatgccg
    20161 ttgattgtat ggcattttta tgagtggcct attttaggta gtctttttac attactgatt
    20221 gcaccggtat tcaagtttct ttttcttcct ggtgtgttat ttctcacact cttcaatcgc
    20281 tggctgccgg aaactttact tttgctgata gaagagggat taatcttatt tgagcgaata
    20341 atcgattttt cttcagggtc gtctctcatt ataggacgtt tgccccttgt gctatctggg
    20401 atgtatattt tcggattact gatttgtatg gataggttga aagaacaacc tgtgaaacac
    20461 ttgttttatg ttggtctttt ttcttttatt tttttgactt tgccattttt acgcttttct
    20521 tcaacgatcg cttttatcga tgtcggtcaa ggagacagta tctttttgca aagtcctttt
    20581 cgaaaagaaa cgattttgat cgatactgga ggtcagttac actttgaaag agaaaagtgg
    20641 gcgcaaggta tgatacgtcc tcctgctgaa agaaatatcg ttccatatct aaaaggtcag
    20701 gggataagtg taatcgataa actcatcttg acacacgacg atacggatca tgtgggagaa
    20761 ttacccacgt tatctcagca ttttactatc aaaaaaattt atatcggttg gggcgctgga
    20821 cgaaatgagc cattacaaag gcatttatct gaagctcaaa aaaatggtac tgagatcatt
    20881 gaaatcaaac atggggacag gatcagcggt tattatgatc tttatgtatt gactcctttt
    20941 gaaaagggag agggaagaaa tgaagattcg attggtctat ggatggaata taacaatagg
    21001 cgctttttat ttttaggaga tttgaatcaa gcaatggaaa aacaattgct tttgatttat
    21061 cctaatctga aagccgatgt agtcaaatta ggtcatcacg gtagccgtac gtcaagtaac
    21121 acagattttc tttcccatat tgaagcaaaa catgggatta tatcctgcgg cgtaaataat
    21181 cgttatggtc acccgcacag cgaagtattg gagacactag aagaaaatca gataaaaaca
    21241 tggcgaacgg atcaacaagg aatgatcacc tatcgctggc atccagtttt tcatcctcat
    21301 gggctagtgg aaacaatgat agactagtaa ttctcatctg ctcatggtag gataattttt
    21361 gaaagtggtg agtaatttga gtatacaagc agagttacaa aaaatacgta aagaaaaact
    21421 tgcatcttgt tacttgattt taggaacaga aaagtttctc caagatcaag ttcggacaga
    21481 aattgtcaaa aaaatccaat tagagggcga agatgactta aattttttaa gttttgacat
    21541 ggaaaatgca agtttagatg aggtagtagc tgaagcagag acgttgccgt ttttcggaga
    21601 tcaacggtta gtattcgtag agaatcctta ttttttaaca ggtgaaaaag taaacaatgg
    21661 aatcgaacaa aatactgatt tattgacggc ttatttgaaa gaaccattag aatcgactgt
    21721 attagtattc tttgcgccat atgaaaagtt ggatgaacgg aaaaaagtaa cgaaacaatt
    21781 gaaaaaaaca gcaattatca tcaatgtcca acagttgaat gaaaaagaag tacgtcaata
    21841 tctgatgaat accttagaga attcctcaat caaaatggaa cgaagtgcaa ttgatttatt
    21901 cttgaggttg accgacttag atttatccaa aatgatgaga gagttacaga aattggtcct
    21961 ttatggacaa aatcaacagc aaatcacggt aagagaagta gaagaattag ttcctaaaac
    22021 gttagaacat aatatttttg acatgaccca atatatttta aaagggaaga cggaacaagc
    22081 tttgcgttta tacgaagatt tagttatgca aggggaagaa acaatcaaaa ttaatgcgat
    22141 tttgctttct caattacgtt tatttttaca gacaaaattt cttgtaaaga ttggttatca
    22201 gcaggcaaat attgctgaaa cattgaaaat ccatccatat cgtgtaaaac ttgccatgca
    22261 ggaagtaaaa aaattcgatg aacatttgct ggtacagttg tttgatcaat tagttgagat
    22321 ggattatcaa atcaaaacgg gacaaatcga aaaagaatta agttttcaac tattcgtttt
    22381 gcgcacaggg cagttgttaa gaaatcgata agaagaaaga cgagggcaaa gcatgcggga
    22441 aggtcgtttg tttttgtcct ttttttgaca aataaaaaaa tcggccgaag ccgattcttt
    22501 tatttagcga tttttttgct taaacgagat ttgtcgcggt tagctttgtt tttgtggatt
    22561 agaccttttg attcagccat atcaatagct ttaacagctt ctttatataa cgcatctacg
    22621 ttgtcagcac ctgaagctac agcatcttca aattttttga ttgcagtacg catagcgctt
    22681 gtttgagatg aatttttcac attagcgttt tcgcttgtac gaacacgttt gattgcagat
    22741 tcaatgtttg gcattagttt cacctccgat aacttaagtt cttacgcgtg ttaccgcata
    22801 attcaacatt cattattata cctaaaggca gcgtgctttg caatatgaac atgacaagtt
    22861 tttttcctta acagtctttt attaggattt tttttcagta aaaacggata gtacaagctg
    22921 aaaaaaatca aaaagacgct attgtgggag gcaatagcgt caaaccaaac tagattttta
    22981 tttatctatt taaacaaagt ttgaatggga atgtttggta ctaagttatt atataacaaa
    23041 gagaaccgtt tgcaaagttt tttttgaaaa gggatacgat tccctgttgt tctttgtttt
    23101 tctattaaga aactgcagtt attttataac cagtatcctt tgatcagtaa aggctattct
    23161 ggtgtaccga ttattttaat gtattcatcg atatttttta tcaacatttt ttttatgacc
    23221 aactggttcc gacggttcca tttgccactt acttccagca catatttatt taggggcaaa
    23281 cgtaagatat attttgccaa atagcgatct ttaagcaggc cattgatcac acggtcctca
    23341 agctgcaagt tgatcccagt cagcatcatt ggctgttctt gtaaaacgta cgtatcaatt
    23401 aaaagacctt caaaagtttc cacaagcgtc ttcctttctt ttcaaagtaa cctgaaaaca
    23461 ggcactaatt cttccatttt gatattcgtt gaagtggatg cgatcattct ttcttttgtc
    23521 cactgtaatc tagtataaca aaaaagagca gaagagccaa aaagaaaaaa gagcatcgaa
    23581 ttctatagga agtctcattg gcacggccta aattattaca aaacattgat taaaaaaagc
    23641 ccctgcatcg caggagcatc ccctatatcg ttttaatagg tcaatcaaaa agcagtatca
    23701 aacacatttt gtatcaaatc aggaaataat agataagcta agcccagaat caaaagtacc
    23761 gctagaagaa accagccgat tgttcctatg aaatacttta atttttcgaa cattgtatca
    23821 cctcgttgtc agtatatcac ggatcagtaa attgggggtg ttaagaatct cctaaaatag
    23881 acaaaatttg ttctattttc ctaaagcaaa tacttgtaag atacagtaag tgataccaga
    23941 agcaatgacc ggtccggcgg cgattcctct taagaagaca acacctaaaa tcgtgccgaa
    24001 aacaagtgca acagtgattt gtggatcaga agcaatcaat cctacaccct tggaagataa
    24061 gacagccact agcccaccgc aaaaaacagc tacccagcca ataggggatt taaaggcttc
    24121 taatagatca cgtagaccga tttctcctgt tgcgataggg attagaatag ccgctgtgat
    24181 gatcgtcacc ccaatattga ttcctttgcc ttgaagccaa gggaaaaagg aacgggaggc
    24241 tggtatgagc ttcataatca aaacaaaagc tgccgctatc atcagacttt gatttttagc
    24301 taaaattgct acaagtaaaa taattcctaa aaaaagccaa ctttccatga aatatattcc
    24361 tcactttctt cgaaacaatc ataacatgtt tttagaaagt tacaaaagta ggaattcatc
    24421 cttcaagacc tattcataag ctgttttttt cataaaataa acaaaatcat aaaaaaacac
    24481 tttgcttatt tgacagaaga cagccgcgct ttgtatagtg gacaagactt acattttttt
    24541 gaagaatcag aaagatcagg taaaggtctt agtaaacgca agccattcag aataaccaat
    24601 attgtgcttc cttcgtgtcc tacaactcca agaggtagat ttaaaaattg caggaagtta
    24661 gaagcaatca acaagagaat aacaagaatc gaaaaaataa tattttgttt gatgatccgc
    24721 cgcattttta aagatagccg atgactgtag acaaatttat ttaagtcgct tttcattaag
    24781 acgatgtctg cgatatccat agcggcatct gtcccttgcc ccatagcgac acctaaagaa
    24841 gcggctgcta aagcaggcgc atcattgact ccatcaccaa ccatcgcatt tacttggtaa
    24901 agttcattct cttttttgac tagttgtgtt ttttcttccg gagtacagct tgcatagtaa
    24961 tgttcaatcc ctacttgttc agcaatggcc tttgctgtgc cagaatggtc gccggttatc
    25021 atacttgtat ggatgttttg ggatttaaag taggaaatta cttgttgggt atttgctttt
    25081 ggaatatcta gcagacctaa aacagcaatt aactgctgat ctttagaaag ataaatcacg
    25141 gttttgcctt gattttccaa acgttcgatc atttccgcaa tctctttgct gattatcatc
    25201 gtctcaggat catatgcgtg cttgccaact ttccattgcg cacctaaata ggtagtttgt
    25261 aatccaaaac cagtaatttc ttctattggt aagtattgga tttcttcagg tattgtgcaa
    25321 tcgaaatatt gcaaaatcgc atgagcaagt ggatgcgtcg ttttttgttc catagcaaca
    25381 agaagttgtt gtgccaaatc cttttcatca aagaaaaagc tgtcagttac aactggtttt
    25441 cctttagtga gcgttcctgt tttatcaaag gcaatcgctt ttaattcaga aagcttttct
    25501 aaatggatac cgcctttgat taaaatccca ttttttgcag aatttgaaag agcagctaga
    25561 gaagctggtg tagcagatgc gacaagcgca caaggagaag ccacaactaa taataccatt
    25621 cctcgataaa agctttcatc ccaactccaa cctaggaaaa agtgggggag aaggatcatt
    25681 agaggaacag caagcaaaat caattttaca taattatttt cgattcgttc gataaatgaa
    25741 gctgtttgag ttggcgtatt ctgcgcttct tctactaatt ggataatctt agcaaagaca
    25801 gtttcggaac tcgtttttgt tactcgaatc gtgatgggat ttccttgatt cagcgttcct
    25861 gcatacatct catcgcctaa atatttttct acaggcacag attcaccgct taatgcagac
    25921 tcatcgatcg ttgcctgtgt gctttctaaa aagccatcta tcggaacagc tgctccttta
    25981 gcgacaaata ccaagttatc cacctttagt tgagaaacat ctacttcttg catttgtcca
    26041 ttttctttta ataattgggc tttttgtggt tgaagattca tcaaagcagt aatttctttt
    26101 tgacttttat tagttgtgta ttcctctaat gcaccgctta aacaaaagat gaatgtcaac
    26161 attgctcctt caaagtaatt accgatcaaa caggcaccaa tagcagctaa agccatcaat
    26221 aaatccacat tcagtgtccg atcattccac aattctttca agccttccga tgtttgttta
    26281 tatccaccga tgataatggc actgatataa aaaagtgggg tgatggtaag agagaatcgg
    26341 tcgaagataa aaccgaagat cataaataaa aggcagccga ttgttgctgc aaaagctttt
    26401 ttctgattag tcatcgttat tagacctcct tgtcaaaaca aggatagcat aataaaaatg
    26461 taattgataa taataacagc taaatgagaa cgataatggt tatcatttgg tgattaagct
    26521 tgatgttgtt tttattattt ataatgatta ttattaaaga aaataaaaaa ccagaacatt
    26581 tctcaaataa gagaagtgtt ctggtttttt ctacattgca ataaaccaat aaccaatcaa
    26641 aatgatacca aggataatac gataccagcc aaaagcggta aagtcgtttt tcttcaaata
    26701 gcttagtaag aatttgatag ctaagacgga aaccacaaaa gatacgaatg ttcctacaag
    26761 aagaacaaca gtttcttcca ttttgaatga attcccttca gctaaatatt tgacgatctt
    26821 caagccgctt gcgccaaaca tgacaggaat ccctaagaaa aatgaaaact ctgtagcgac
    26881 aaaacgcgaa gcaccaatca agatagatcc taggatcgtt gcgccagaac gggatgttcc
    26941 gggaacaagt gccaagactt ggaataaccc aacaagaaga gctgcttggt acgtaaagtc
    27001 attcagagaa gtccaacgag gtgttttgtt tttgttgcgt ttttcgacaa taataaaggc
    27061 aataccgtaa acgatcaaaa caatggcaac tggtaagaac ttgtggaaat ttgcttctaa
    27121 ccaatcatcc aaaggcaaac caatgatgat ggctggtatg caagcaacga tcactttaaa
    27181 ccaaagtgtc cacgtatctt ttttttctac cgttgattta cttggagaaa atgggtttaa
    27241 cttgtgaaaa taaagaacga cgacagccaa gatcgcccct aattggataa cgacattgaa
    27301 catcgacata aattgtgaac tggcatccag tttgatgaat tcatctgcta ggatcaaatg
    27361 accagtacta ctgatgggca gccattccgt tattccttca ataataccga gaatcactgc
    27421 tttgataata tttgcaaaaa acataaattc cttccttccc aattaagata catcttatag
    27481 tataccgtta ttttttgaga aacccaatct gttttttaat attaagaaaa acttgagaaa
    27541 taaagcagat aagtacacga agagaaaaaa gaagacaaag ccctcgttca gactttgcct
    27601 tttatgattc tctggtaaaa attaaaatat ccccattata gaaacgagtc gcgactatgt
    27661 gttttcccgt tatcgatata agacgtatca ttgatgccgt ggatagaaaa tcgaccgttt
    27721 gcataagtga cctttgttac gctgccattt aaaagcccag cacgaacagg ctgtttagga
    27781 tcgatcagat taagcaaaaa ggcgatcgtc aaaccatgag atacgactaa aacatttcca
    27841 ccaccatttt tttcgcagtg atgggcgata tcttcaaatc ctgtccatac acggtctcgc
    27901 aatgtttcat aagtttccgc ccacccagcg gtatctaagt tgtatacagc attagcaatt
    27961 tgttcaaaag aaacttctgt ggtaaacatt tcatcatatg ttttaaaatt caatacacga
    28021 gggatcacac cccacatttc catgtcatat ccaccttcaa gagacccgaa acaccattct
    28081 ctaatccttt tttcaataaa ataaggaatt tcttttccct tagaatgttc tcctagaata
    28141 atacgggcag tttcgattgc acgaccactg tcactagaat aagcaaatga aaagtcgaca
    28201 ttttttaacc cgcgacctaa ataatgaatg ccttcttgtc cttgctttgt gaggggtgta
    28261 tcacaccagc cttgtactcg ttcaattgta ttgaacatcg tttttccgtg tcgtacgata
    28321 taaagtgtag tttctgtcat tttattctcc tcctaagtcg ttttaacgat tgaaaaaagg
    28381 atttgtcctt ttttcctgtt caatcgtcgt tgcatcgcca tgtcctggat agactgcaaa
    28441 ttcatcaggt aatacaaaca gctgggtccg aatactatga agtaattgtt ctaaattccc
    28501 tgtatacaga tcggttctac cgatacttcc tctaaacagg gcgtcgccag aaacgacaaa
    28561 atcatcaaaa ataaagctga cgcttcctat agaatgccct ggggtaggga cgacgcgaaa
    28621 agacatgttt cctaaagtat aagttttcat ttcaaattca tattctgcag gcgagacaat
    28681 aacatcagca atatcatcat gtctaccgag tccagaaaga ttcaaaatcg gatcacctag
    28741 ccatttttgc tctagtgggc tgacataaac tgggatttga taatacgcgc gtaaaggttc
    28801 cactgcaccg atatgatcgt agtgagtatg ggtaagcaaa atcgctaccg gctgttggtt
    28861 tgtttttttg atttcttgtt tgattcgttc cgcatcttct ccagggtcaa tgatcaaaag
    28921 tgcttcttcg ttgtagacca aataacagtt ttcttcaatc attcctgttt ttaatctttc
    28981 gatatgcatg tgcattcctc cttgatagcc aaaacttctt tcatccatta gtataacgca
    29041 ttcattgcta ggaaacaaaa acaggtactt atttctcttt acttatctga attatcagac
    29101 tatatgaaaa gtatttggat attctaaaga gaaggaaagt aagttttcat agcattttaa
    29161 tatgtccttg ttacattaga ttcatccaaa aatccaaatt ctatatccta tatccctgcc
    29221 taatcagcag ggtgtttttt tatgtttgga aagagagctc tttttgctaa actaggtttt
    29281 ttcaggtaat cttagagaga aagaggtggt ggaaatgatc aaacagtcaa aaatatctgt
    29341 gcggcactta gtagaattta ttttgcgacg tggaagtatc gacaatcgta aaaaaagtaa
    29401 ccatactgca ttagaaggag cgagaatcca ccgcaagctc caaaaagatg cgggagaaaa
    29461 ctatgaaaaa gaagtctttt taaaaacaac agttcaatta gatgaatatt acctgatcgt
    29521 tgaaggccgt gcagacggta ttttcaaaag agacggtatt tattatatag atgaaatcaa
    29581 aacctccgaa ccacgatttg aagatttaga accagaacaa gtggaacttt tctttcatca
    29641 agcaagggtg tatgcctata tctatagtca agaacatgat ttacaagaaa taaatctgca
    29701 attgacttac ttccagacgg cagaagaagt gatcacgaga aaggttgaac atcagacaag
    29761 agagcagtta gaaacttttt ttaaaaagct gactgaagat tataaagaat ggcttgtttt
    29821 tcaagaaaac tggcgcactg tacgcaatgc ctctttgatg gctttgaaat tcccccatga
    29881 tgagtatagg aaaggtcagc gtgaactagc ggtagctgct tataaaacga tccgtacgaa
    29941 acagaaattg tttgtagaag cacctaccgg aacaggaaaa acaatctcta cgttattccc
    30001 cgctttgaaa gctgtagggg aagaagaggg agaaaagatc ttttatctaa cggctaaaac
    30061 aataactaga caagtagctg aagatgctat gacagcatta aaagatacag gagctgaagt
    30121 aaaaagtgtc actttgacgg cgaaagacaa gatctgcttt ttagatgaga cgacctgtaa
    30181 tccagatcag tgcccatatg ccaatggtta ttacaatcgg attaatgaag gtttatggga
    30241 tcttttgaat catgagaatc agatcactcg tgaagtcatc gaaacttatg caagaaagca
    30301 tacactttgt cctttcgaac tatcattaga tgtcagtatc tggtgtgacg taatcattgg
    30361 ggattacaat tatttatttg atccaactgt ttatttaaga cgtttttttg aagatgaaaa
    30421 aaacgaggac tatctgtttt tgattgacga agcacataat ttggtgaatc gttcccgaga
    30481 gatgtattcg gcagaactat cttatgaaaa aacgaaacga agtaaggaag ctataccaaa
    30541 ggaattcaaa aaacttcatc gccgtttcaa caaactatta aaagaatttg attcgatccg
    30601 agaaatagca aaagaagatc attgggatta tcatcatcaa aaagcaccgg ctgaatcttt
    30661 agtaaaggca ggttatcaat taagcgaaaa aattaaagaa tggctggcag aatttcctga
    30721 gcacccatca caagaacaat tgcttcctta ttattttgat ttgcttcatt ttttgaaggt
    30781 cagtgaattt tatgatgatc actacgaaac aacagtcgaa aaaacgtatc gcgatctgat
    30841 agtaaaagag ttttgtattg atccaagctt gtttcttgag cagtcgttgg ataaaggaaa
    30901 aagcagtatt cttttttctg ctagtttttc accactctct tattatcagg aaacactggg
    30961 gggacaaaaa agtctggctt acaaactgcc gagtcctttc ccagaaggaa accagcaagt
    31021 tttcatcgca aattacctag agacgactta tcgaaaaagg gaacacagtc tggcaaaatt
    31081 agtagagacg attcatgctt ttgctgatgg aaaggtcggg aattatttcg ttttctttcc
    31141 ttcttatcaa tatttagatc ttgcggtgga agcattcaga cagcaatatc ccgacagcaa
    31201 tgtgctaatc caagaaacgg acatgaatga ggaagaacgc gaacgatttt taacaaaatt
    31261 tcaaacaaat cccgctgaaa cattgattgg attttgcgta cttggcggga ttttttcaga
    31321 aggaatcgat ttgagagggg accgattgat cggcagtatg atcgtaggtg taggtttgcc
    31381 gcagatgaat catgagcagg aactgatcaa atcatattat gatgagaagg aacaccttgg
    31441 atttgcttac gcttatcagc ttcctggaat gaacaaagtc ttgcaagctg gtggtcgagt
    31501 gattcgtggc atggcggatc aaggaatcgt tgtattggca gatcaacgat tttctagttt
    31561 ttcttatcga cgtttattcc cacaacattg gcaaacagca agagaagtac gaagtgttgg
    31621 ggaattggaa gaaggaatac agcgtttttg gttatcaaaa gaagcagtaa atcaaaaaaa
    31681 cgaggaatga cagacgtcag cctcgttttt tgattaacga tagttagtga attgcaactc
    31741 gattggtaag tcaagtttac gtaataaagc aatcacagct tgtagatcat cacggttctt
    31801 acctgtcact ctaatctgat cttcttggat ttgtgctttt actttcagtt tttcattttt
    31861 gatagctgta gtgattttct tcgcgttttc acgatcgatt ccgttgacca aatcggcagt
    31921 ttgtcgagct ttgccaccta gagcgtgttc tgattcggaa aaatggatat ttttgattgg
    31981 cacatttctt ttgatcagct taccaaataa tacatctttg atctgttcga ttttgaaatc
    32041 atcatcgctc aatacaacta gattgctgtt ctctaatttg atttctactg tagaatcctt
    32101 gaagtcaaag cgattggaca attcttttgt tgtttgctga atcgcatttt ttacttcttc
    32161 tgtattgact tctgatacaa tgtcgaagct agcatctttt gccataggaa ccctcctgat
    32221 atacatataa atatttacca tacatctatc ttatcatagg attagggcag attcttacct
    32281 gtttgctaaa attcttctca tttgagttcg aagtatttta ttttaataga taaactgtta
    32341 ttcttacatt tggaatacca aagtagggga gaggtgcttg tgagtaaaat tatgactgcg
    32401 tatcataaaa atgaaagatt aaagaaaata gtggttattt tgataaccgg attgaccgct
    32461 gcggtcgctt taaattattt tttgattcca gccaatgtct tttctgcagg gatgaatggg
    32521 atcgctcaaa tcatttcctc gcttttatat gaatggtttc atatcaagat agacacagga
    32581 acatttattt ttcttttgaa tatccctgtg tttgtattag ggtttctcaa agtaggaaaa
    32641 cgagcaacga tccttagttt tgtgaatgtt gtatgtgtat cggtgatgac gatgctgctt
    32701 ccagtcggac agatcactga taatattttg atgaatgccc tagtaggcgg tgttttatta
    32761 ggtcttggcg taggattatc tttaaaaatg ggctttacaa ctggtgggat ggatatcatc
    32821 tctcttgtac tttcacaaac aactggaaaa acagtcggaa actacatgct gattctaaat
    32881 ggtatcattg tagtcatcgc aggcttcttc tttaactggg aaagtgcctt gtatacgatt
    32941 atttccattt atgcaatgac tcaagtaatt gatgccatcc acacgagtca tcaaaaagta
    33001 acagcaatga tcgttaccgt caagccagaa gaggtaacag aagaactagc aaaacggatg
    33061 gtacgtggaa tgacattact gccgtctgtc ggtggctatt caaaaaaaca agggaacatg
    33121 atcatgatgg tcatcacccg ctacgaaatg tatgatcttg atcagatcgt tcatgaaatc
    33181 gacgacgatg catttatcaa tattttacca actcattcag tatttggccg gtttgcaaat
    33241 gaaaacgaac aaaaaatgta tcgaacaact ggcgtattgc cagaaataaa aaatcgttca
    33301 caaaaaaaat gaggaatcag gtttatttta gaataaattt aatctattac atctaataag
    33361 aacattttta taaatttaaa cgagaatctt acttctaaat tctcatttac aagcagctaa
    33421 aaaaagagta tgataaataa aaatcgtgtt aaggagtgtc gtacgatgag cgaagcaaac
    33481 caagaacggt ctggacaaga aatagaacta atcaaagaac gtattcttgc tgccttggaa
    33541 atggtgattg atcctgaatt aggaatcgac atcgtcaatt taggcttgat ttatggttct
    33601 ataatatttt gtgttggtgg aaaatcgaaa gcgattttct actaacactt ttttcttcgt
    33661 ttcaactcaa aaagagactc acttttggta aaatttagtc gaccaaaacg tca
//
LOCUS       DAYYKN010000013        32497 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000013 DAYYKN010000000
VERSION     DAYYKN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.013"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..940
                     /locus_tag="ACHBB7_000536"
     CDS             167..940
                     /locus_tag="ACHBB7_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9208512.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            1087..1452
                     /locus_tag="ACHBB7_000537"
     CDS             1087..1452
                     /locus_tag="ACHBB7_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM9208513.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(1600..2808)
                     /locus_tag="ACHBB7_000538"
     CDS             complement(1600..2808)
                     /locus_tag="ACHBB7_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9208514.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2827..3105)
                     /locus_tag="ACHBB7_000539"
     CDS             complement(2827..3105)
                     /locus_tag="ACHBB7_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208515.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            3298..3954
                     /locus_tag="ACHBB7_000540"
     CDS             3298..3954
                     /locus_tag="ACHBB7_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9208516.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(4396..5193)
                     /locus_tag="ACHBB7_000541"
     CDS             complement(4396..5193)
                     /locus_tag="ACHBB7_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM9208517.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(5190..6374)
                     /locus_tag="ACHBB7_000542"
     CDS             complement(5190..6374)
                     /locus_tag="ACHBB7_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM9208518.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(6399..7646)
                     /locus_tag="ACHBB7_000543"
     CDS             complement(6399..7646)
                     /locus_tag="ACHBB7_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9208519.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            7861..9606
                     /gene="dnaX"
                     /locus_tag="ACHBB7_000544"
     CDS             7861..9606
                     /gene="dnaX"
                     /locus_tag="ACHBB7_000544"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM9208520.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            9626..9940
                     /locus_tag="ACHBB7_000545"
     CDS             9626..9940
                     /locus_tag="ACHBB7_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM9208521.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            9965..10561
                     /gene="recR"
                     /locus_tag="ACHBB7_000546"
     CDS             9965..10561
                     /gene="recR"
                     /locus_tag="ACHBB7_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM9208522.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            10625..11269
                     /gene="tmk"
                     /locus_tag="ACHBB7_000547"
     CDS             10625..11269
                     /gene="tmk"
                     /locus_tag="ACHBB7_000547"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM9208523.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            11284..11613
                     /locus_tag="ACHBB7_000548"
     CDS             11284..11613
                     /locus_tag="ACHBB7_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM9208524.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            11627..12565
                     /gene="holB"
                     /locus_tag="ACHBB7_000549"
     CDS             11627..12565
                     /gene="holB"
                     /locus_tag="ACHBB7_000549"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM9208525.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            12601..13425
                     /locus_tag="ACHBB7_000550"
     CDS             12601..13425
                     /locus_tag="ACHBB7_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM9208526.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            13418..13765
                     /locus_tag="ACHBB7_000551"
     CDS             13418..13765
                     /locus_tag="ACHBB7_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM9208527.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            13834..14706
                     /gene="rsmI"
                     /locus_tag="ACHBB7_000552"
     CDS             13834..14706
                     /gene="rsmI"
                     /locus_tag="ACHBB7_000552"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM9208528.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            14815..15936
                     /gene="dinB"
                     /locus_tag="ACHBB7_000553"
     CDS             14815..15936
                     /gene="dinB"
                     /locus_tag="ACHBB7_000553"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM9208529.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(15990..16592)
                     /gene="nrdG"
                     /locus_tag="ACHBB7_000554"
     CDS             complement(15990..16592)
                     /gene="nrdG"
                     /locus_tag="ACHBB7_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM9208530.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(16907..19096)
                     /gene="nrdD"
                     /locus_tag="ACHBB7_000555"
     CDS             complement(16907..19096)
                     /gene="nrdD"
                     /locus_tag="ACHBB7_000555"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM9208531.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            19422..20189
                     /locus_tag="ACHBB7_000556"
     CDS             19422..20189
                     /locus_tag="ACHBB7_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9208532.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            20179..22221
                     /locus_tag="ACHBB7_000557"
     CDS             20179..22221
                     /locus_tag="ACHBB7_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9208533.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            22502..22651
                     /locus_tag="ACHBB7_000558"
     CDS             22502..22651
                     /locus_tag="ACHBB7_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM9208534.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            22662..24176
                     /gene="dltA"
                     /locus_tag="ACHBB7_000559"
     CDS             22662..24176
                     /gene="dltA"
                     /locus_tag="ACHBB7_000559"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM9208535.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            24173..25378
                     /gene="dltB"
                     /locus_tag="ACHBB7_000560"
     CDS             24173..25378
                     /gene="dltB"
                     /locus_tag="ACHBB7_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM9208536.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            25423..25656
                     /gene="dltC"
                     /locus_tag="ACHBB7_000561"
     CDS             25423..25656
                     /gene="dltC"
                     /locus_tag="ACHBB7_000561"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM9208537.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            25659..26927
                     /gene="dltD"
                     /locus_tag="ACHBB7_000562"
     CDS             25659..26927
                     /gene="dltD"
                     /locus_tag="ACHBB7_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM9208538.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            27068..28150
                     /locus_tag="ACHBB7_000563"
     CDS             27068..28150
                     /locus_tag="ACHBB7_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM9208539.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            28150..28953
                     /locus_tag="ACHBB7_000564"
     CDS             28150..28953
                     /locus_tag="ACHBB7_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM9208540.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            29023..29763
                     /locus_tag="ACHBB7_000565"
     CDS             29023..29763
                     /locus_tag="ACHBB7_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9208541.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            29774..30778
                     /locus_tag="ACHBB7_000566"
     CDS             29774..30778
                     /locus_tag="ACHBB7_000566"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM9208542.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(30823..>32497)
                     /locus_tag="ACHBB7_000567"
     CDS             complement(30823..>32497)
                     /locus_tag="ACHBB7_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208543.1"
                     /translation="VFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANL
                     LIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQN
                     YAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYS
                     LILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYK
                     KWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNN
                     RFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILN
                     TDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAK
                     NYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDD
                     DLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGI
                     TEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        10324 a         5722 c         6650 g         9801 t
ORIGIN      
        1 aatgtgtggt caagagggta tgacaatagt cttgtcatac cctcttaacc acacacgatg
       61 ttcagaagta gttttttcga aaaatttctt cttatttctc gaagccaatc acttctgtca
      121 caacctcttt tttcctgtta ctatataaag taaatggggt gattttatgt tgaatgaagt
      181 ggagaataga cagcaggaga ttgtgaatta tctaaaaaac aaccaatttg caagagtcac
      241 cgatttgatc gagttagtaa attatagcga agccacgatt aaacgtgacc ttgtttttct
      301 ggaaaaaagt ggattgatcc gtagagttcg cggtggcgcc atgcttattg acaatcgaaa
      361 aattgacgtg ccttacctga tgaagatcac caaattagaa gaagaaacag aaaaacgtaa
      421 gattgcagat acagcttctt ctttgattcg agatgatatg gtcttattct tggattccag
      481 tacaacctct ttacacttag tcagaaacct agcaagattt gatggtttgc agatcattac
      541 gaatggcatc gtcacagctg ctatgttgag tgaatataca acggcaaaag tatctgtatt
      601 gggaggggca attgttccga aacgagcaac aatcaacggt tcaaaagcct ataacgatgc
      661 attgtcatat tatgcggatt tagcgataat gagttgtcgc gggctagatt tcagccaagg
      721 agtaacagaa actcatgaag gagaggcaat ggtcaaaaaa gcttttagaa aacaatcaga
      781 ccggcttgcc gttttagtta cacagcaaaa aattgcacat aaattcatgc atcaagccat
      841 tgcttttcat gaaatcgatt atctggtgac aagtaaagaa ctgacaaaag aacagtatgc
      901 gaaagtaaag gaacaccata tcgaatgtct gtacggataa attattacgt gaatgttcta
      961 agtggcttcc tcatttttag ataggacctt atctttgctc gcttctctca gccgatggta
     1021 taataatgaa aaggttatct tgttagataa tcttactttt ttatccagga gaaaggagtg
     1081 cagaaaatga aacatgtgaa ggcgttggta gtgaaggcaa tcatgatctg ggcgattctg
     1141 tgggtcgtgc tgacaggttt atacggtgtt agctttatgg attcaacgat tgttggggta
     1201 attattgtag tcatgatcta tgtgctaggg gatctgttga tcttacgtaa agtagggaac
     1261 atcgcagcga cgatcgcaga tgctggttca gctgcagtta tcttgtggct ctatctatat
     1321 tttatgaatg atacagtcga tatttggatg aaagtattga ttcctgctct attgatcggt
     1381 gtggcagaat ggttcttcca caagtggttg ctaagtcaag gcatcgtacc agatgaacgg
     1441 aaaatggaat aaatcaacag ttaaactatt cttatcgata aatgaaagct ttaaaaataa
     1501 atagatgttt aattaagaat aatcataaac tatttttact caaaaaaccg ctcagattat
     1561 tttcagatct gaacggtttt ttttatctat tcatcaagat caaatttcat ttttcaatga
     1621 ttcaatcaat tcgccctctg cttcatgact atgttttcgg ccattaacaa gatagagtgc
     1681 tgtacatatc aatggaatga ctccggcaat cacatacaat cctctaaatg aagtaagaga
     1741 ttttagcatc cccatcaaat aaggaccaac gcctaagcct aggtctaaac caataaagta
     1801 ggtagataaa gcaatactga ttcgatgttc gcggacgatt tttaaacaaa cagcctgtcc
     1861 atttgacata aacgtaccat aacctaggcc gactaatgct ccagagataa gcaatgtcac
     1921 actattcgtc gttacactca gtaaaaataa tcctgctgtt aagaataaat agctaggata
     1981 catcacgtat tgttctcctt tgacatcgaa aatccttcca gtcaatggac gtgtaaatgt
     2041 gatgaccaaa gcatatacga cgaagaaaaa tgacccagca gcaactaagt ggattacttt
     2101 tgtataagaa gataaaaatg ataaaacact tgaataagaa agacccatca aaaaggcaat
     2161 tcctgtaatg aaaagtgctt tcttttcaat aaaactattg atattccatg agcgaagaga
     2221 tattttttgt tcttcagata atggaatatt ttttacagga aagacaaggc aggcaatcgt
     2281 aacaaagaag accagtacaa ttgaaaacca gataatgaaa cgaaaatcag tcatattcag
     2341 caaaatcatt cctaaaaatg gcccaacagc ggcagctaaa ctagtactta atccataata
     2401 attgatgcct tccccttttt tagatgcagg gatatatgca gtaacgatag catttgtagc
     2461 tgtagaaact gtaccgtagc cgaatccatt caagaatcga actaaataca atgccccgat
     2521 agttggcgta taaagatatg ctaatgttga aaggagatag aagatcgcgc cgcctcttaa
     2581 ggtaaacttc cgaccaaata gttctaactg tttccccatg aataagcgtg ccagcaatgt
     2641 tccgatgata tagatcccag aagcaaaacc tgcttggctc aagctggcgt gtaaggtgtc
     2701 ttgcgcaatg actgccatga tgaccatcaa cagataataa atcaggtaga caataaaatt
     2761 gatcaatgtg attccaataa aatatttatt aaataatttc ttttccatgt atgttataca
     2821 ttccccttaa gctgaatatg cttttaattt ttgtatttga acttctacat cgtgataatt
     2881 ggatggatag cgatcaacaa gttggcgaat aaataaatct cttgtagaca taaacagatt
     2941 taaaaattct tcgcattcgt ttgttggatt ctcatccact gctttgatca attcgatcga
     3001 gttttgatac gcatcattta aaaagactaa agaagctttc atatcaaatc ttgtttgata
     3061 tgctgtttct aatcgattaa tgattgtgtt catccagtaa ttcatgtttt ttccctctat
     3121 tcttaaaaaa ttttacttct gcagtataac gaataaaaaa aaggaaaaaa atcgcataag
     3181 tgatggaaaa aggtacttaa caaatgttaa tatcattagt aaaatacctt tggactttaa
     3241 aaagttttaa agtactgtat gatcttttta gatgaaagtt tggatggggg agcatcattg
     3301 gatagtagag tcttaaagga atatttggat aaaaatgatt ttccgattat acgcaaagga
     3361 tatcataaat atttgacatt cgaagggatg gaagattccc atacgtatat cttgaaagag
     3421 ggaacggtaa aagcaagtgt tatttcaaat gatgggagag aatttaacct tcgctatatc
     3481 aaagatcttg aaatcgtctc tcttttaaaa gacgaatatt cacaattcat cgattcgccg
     3541 tataatattc gagttgaatc agaatatgca gagttttatc gaatcaatcg tgtgcgcttt
     3601 tggcaaaata tcaaccaaga cccaaagcta caacactatg taaaagagta ttaccgctat
     3661 cgtctgcttt attcgatgaa aaaaatgcag caaatgctat taaacggaaa gctgggagct
     3721 gtttgtacgc aactttacga attatatgat ttattcggtg tgcggacaga agaagggatg
     3781 ctgatcgatt tttcgatcac gaatgaagaa atcgccaaat tttgtggcat ctcaactgct
     3841 agcagtgtaa acagaatttt aaaacaactg aaaaaagatg gtattatcac cgttaaagac
     3901 catcatatcg ttattttgaa tatggacttg ttagaagaca atattatctt ttgatgaaca
     3961 aagaggttgt gaaatcagcg ttttgacctg agtattagag aaacaaacgg aaaaatagtt
     4021 cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttac
     4081 caagaggttg tgagcctgcc gtttaactct gagtcacaag gaacaatttc aaaaaacagc
     4141 ttttcatgtt tgatgaatat tgtgacttgt gaccgaggag ttggtaggag aacaccgttt
     4201 accaagaggt tgtgaaaaag ctgttctgca tcaagtaata agaagagcca agtaaaaaca
     4261 gtctcttatg tttttaactt ggctggactt atcaccgtag atgtaagctt ttgaacgccg
     4321 tttatttgca acaaaagggg ctgtgacacg atttttgtca cagccctttt tttgcgaaca
     4381 ttcatttagc aatttttaga aacttctgcc gccaccgcca aaagtcccgc caccgctaga
     4441 atgggtagta cttccgccac cgccgcttcc aggaggtggg cttttgggga ttcttcttgt
     4501 cgtgacaaaa gaatttgtca gacgatcggt tttttcagtt aatttgatac tggatttttc
     4561 tcggaatgga tatttgtaga tgcctgattt caattgataa cgcgaaacag tcacaaaata
     4621 aaagacaaga ctaaggataa gagccaaaac aacagcaatg atgatttcta ctgtcgtcaa
     4681 aaccttatag cgtgtaattt tgccagtttc ttcgtctaca cgataatgtc ctcctggaac
     4741 tccgtcttcc acatatgttg ctgatttaga caggtaagct tttgcagcat tgtaataggc
     4801 agcatttgtc atctgatcat aaacatcatc taagatcgaa tcgattctgc tatcagtcaa
     4861 ataatcgatc atattaccag aagtcgaaat gtagatttca cgctgtccca tatctaatag
     4921 caatactgag ccattttgat catttccgac tgcgtctctt aagtaattat cagcaaagtc
     4981 tcttggttct tctgaattag atgtagtcgt tacgatcaag actcgtcctt tgattttttc
     5041 attcagcttt tctgcctgtt gttccaaaga ttggatttga tctgttgtga ataacccagc
     5101 ctcatcattt accgtagctg tatctgcatg agtagccacc ggccagccaa cacttactag
     5161 caaaagaaag aataaaaaaa attttttcat catatcaagt aacctccgat cagaaagagg
     5221 atcgctagcc cagcgaaaat cccaagagcg gtcagtccca attttttctg gctgatcggc
     5281 aggacaccgc tgacctttcc tgtttgtcca ttcattgcgt agtagtagac ttttttactt
     5341 gagtcattgc ttcgataagt aacaagccat accggcagta atacgtaatt atttacttcg
     5401 tttgtgattg aagcagaagt tcgcacaccg gtcaatgttg tatatccatt tgcggtatct
     5461 tttaacagct tctctgaata gtcttttaat tcagattgaa tctgtgattt gattgcctca
     5521 tattcgatat ctcttttttc tgcttggaac ccggctaaat actggctttt aaaatcaatt
     5581 gctttatcta acgggaaagg ttgcacgcct tcgaccatct tttgttgcgt attttttgat
     5641 aaggcatttt ttactagttc tttaaaagaa agcttgcctg ctcttgaaac agcaaattgc
     5701 ttcgtttctg tgtattcgat gtcacctatc cgccatatgc ggataactgt accattcgcc
     5761 tgcatggcac catctacttc agcatctgta gcccaataag ggaaatacac acctgtcaac
     5821 ttatcgattt gatccttatt gaaaaaagct ttgggaacaa accatttttt cttggtccaa
     5881 gcgagaaatc tgttgattgc ttcttctttc tcgacagcaa aaggaagtac tttatttggc
     5941 aagaattctc cgcttaaacg tcccgatagt acgacgggat tatggcagta gtaacaataa
     6001 gtcgcagcag ttgtcgcatc agtcacgatc tctgcaccac agttgggaca aagaaaaaga
     6061 tctatcgctg cggtattatc tgcagattcg tccgctgtat ctgaaaaacc gcctgcctcg
     6121 tcaaccgctt tgcgctcggc ttcattcata tcggcaagct gttcttgggc tgtaaaagta
     6181 aaatcttcgg taggtgaagg ttcttctttg ctttcttcgc cagccatacg cgcttctttt
     6241 tgcttttgct catattggct gacttcttct tctgtataga tattcaggca gtattcgcaa
     6301 tgaaattttt ggtctttggg atcaaaagtc aacggaccgc cgcaattagg acatttatgt
     6361 gtaagaacat ccatcagctg cacacctttc taaataaatt aattaacggg gattccttta
     6421 aacggtttac cacagtttgg acagaattta gggatgccgt tggaaagatc cacgatctca
     6481 ttgcattcac tgcatcgcat ttgtagctta ggctgaacgt tttctacagg tttggcagct
     6541 ccgcagttgg aacagaattt tccggaattt tctgtcccgc aaactggaca tgtccaagta
     6601 tccgcatttc cttgtgcagt ttgttgattt tgtttttctg cttgttgttt gatttgttct
     6661 tggttatttt gagctgcttg agaaagataa gaaccatttg cattcattcc catacctaca
     6721 cccataaagc cggtcattgc tcctgcttca ttttttccgg ctgcttccat accacgtgca
     6781 atggaacctt gcacgtagcc ttcacgtacg ctaggatcac ctagcatcgc cccttcgtta
     6841 cgcatattga tcagtttcac agaatcatcg gtgtaagaaa tgctggctac agccacagaa
     6901 acaatctcca tcccacgtaa ttcacgccaa gaatcatcca atgcagtatc catgtatttg
     6961 cttaattcca aactcttaga tggtacataa gaaatccgtt gaccatctgc agacatctga
     7021 ttgattgctg attgtagcgc tgttaggaat tcagctaaat actgctcatt gatatcattg
     7081 atttccactt gtgttttgtt tttagggatt gcgtttgtat aaaataaaat cgggtcaacg
     7141 acatgaatag aataagtgcc atgtgcccgt aagaataatt ctgcgttata gaaattgtca
     7201 aagtagttca acggcgcgct tgtaccgaat ttgattccct ttatttcttg gagattgata
     7261 taaaaaactt gttgtttctg cggtgtgacg ccgccaaatt tgaatcggtc aaaagtttca
     7321 gcaattgctt ctttcagtgt tccattgaac atggaaggag ctgcgtcatt tttaatggtg
     7381 taataacctt cctcagcggt ataatcgatg atttttccac catcgaccaa aagcatcatc
     7441 atgtttggat agacatggac gactgttccg tctgttaaaa catcttctgt tcctttacga
     7501 ttggagccac gtttgtcatc tttgcggact ttgacacctt ttgtcatgac cgttgtatca
     7561 ctcatattgt ctggttcgat gacttctagc cattgatctg ccagaccgcc gccaaccata
     7621 cttgttgctg ctttgataag acccataaaa aattcctcct ctgattcagc tagtgccaaa
     7681 caattattgt ttatttatta taccatagga agatgtgaaa cttctcggac tagagacgga
     7741 attgaaatgg aaaattgcgt attgtttaaa tacctgaaaa agccgagaag gatagtagtt
     7801 tttttaccta atatcacgta taataagaga tagtgaattt tttaaaaaag ggggatcctt
     7861 atggcatatc aagcacttta tcgagtctgg cgttcgcagc gtttcgaaga catcgttgga
     7921 caaaaagctg tgacacagac actaaaaaat gctattgttt cccacaaaac ctcacatgcc
     7981 tacttgttta caggacccag aggaaccgga aagacgagtg cagcaaagat tttcgcaaaa
     8041 gcaatcaact gtccgaacag taaagacggt gagccatgca acgaatgcga aatgtgtcga
     8101 tcaattactg ctggtacgca agaagatgtg atcgaaatcg atgctgccag caataacgga
     8161 gtcgaagaaa tccgatttat ccgtgatcgg gcaaattatg cgcctacaaa ggcagcgtac
     8221 aaagtgtaca ttattgatga ggtacatatg ctttcaacag gagctttcaa tgccttgttg
     8281 aaaacattgg aagagccaaa agaaagtgtc atattcatct tagcaacgac cgaacctcac
     8341 aaaataccag ctacgatcat ttcacggact cagcgatttg attttaaacg gatcaatacg
     8401 gcagatattg tcgaacatct tgaatttatc ttgaaagaat cgactatcag ctatgaacca
     8461 gctgcattga atgtgattgc ccgttctgca gaaggcggga tgcgggatgc attgagtatt
     8521 cttgatcagg caatctcgtt tagtgaagga acaattactt tagatgatgc catgcaagtg
     8581 acaggtagct taactgacga aatgatggac agttacttga gtgcttgcgt gaaaaaagaa
     8641 gtaccagaag cattggaaac attgaaccaa attctcgcag ccggcaaaga atcacgtcgc
     8701 tttctagagg atatgttaca atactgccgt gacttgctga tgtatcagca agcacctaac
     8761 ctagttgagg aacaatcgag tacgatcaga agtacattta cagaattggc acaaactgtt
     8821 tcagcggatc agctgttttc aatgataaag attttgagcg atactcaaaa tgaaattcgt
     8881 tttaccaata gtgccgctat ttatttggaa gtcgctacag tcaaacttgc acagctaccg
     8941 tctgcttctg ttccttcagc agatgtagca tttgcagatg atcagatagt aaaacagcta
     9001 caacaagaga ttaaggaact aagaaacgaa atcaaggaac tacgcagtaa tacaggaatt
     9061 gagaatacaa aagtacagcc ggtacagcaa acaaatcata aaaaacagac aagtacctat
     9121 cgtgtaccga cggaacgagt atataaagtt ctgcgggaag cgacaagaca gcatctgaac
     9181 caagtaaaag aagtatggga agacttgctg atgagtctat ctgtcactca gcgagcaatg
     9241 cttaaagcaa gcgaaccagt tgcagctgga cctacaggac tagtgattgc gtttgattat
     9301 gagattgttt gtcaaagagc agcaaatgac gaagaattgc agctttcggt acacaacaac
     9361 ttaagccgaa tgatcaaaga ttatgcgcct gatcctgttt atattacaag agaaagctgg
     9421 ccagaacttc gaagaaatta tttgacgcag gcacaagaag agggaacaga aaactctttt
     9481 gcttctgaag actcagaagg agaaatcgag ttgcttcctc aagaacaaga agtaccagaa
     9541 gaaacacttg tagtaaaagc agaggaactg tttggctccg aagctgttac agtggtagat
     9601 gattaataaa aaggagtaga taattatgat gcgtggaatg ggcaatatgc aaggcatgat
     9661 gaaacaagta caaaaaatgc aaaaagaaat ggcagaagca caagagctgc taaataaaac
     9721 agagtttaca ggtaaagcca ccaatgaatt ggtcaaagcg gtttttacag gcgatcgtcg
     9781 catgaaagat atccaaatca atgaagaagt cgttgatcct gatgatatcg agatgcttca
     9841 ggatcttgtc gtaatggcag tcaatgatgc gatcgcacaa attgatgtgg aaactgagaa
     9901 aaaattaggg aaatacgcaa aaggtttgcc aggattttag ttttaaactg aaggagtaat
     9961 gaatatgcaa tatccagaac caattgcgaa actgatcgac agctacatga aacttccagg
    10021 aatcggtcag aaaacagcga caagactcgc cttttatacg atcgatatga aagatgaagt
    10081 agtaaatgaa tttgctaaat ctttgttaag tgtaaaacgc gatttgcatt tttgtagtat
    10141 ctgcggaaac atcacagaag aagatccctg cgaaatatgt agaaatcctt cacgtgatcg
    10201 aagcgttatc ctagtggtcg aagagccgaa agatgtaatg gcaatggaaa aaatgcgaga
    10261 atatcacgga ctttatcatg ttctccatgg tgtgttatca ccgatggaag ggacaggtcc
    10321 agaagacatc aatatttctt ctctgttgca gcggctgcat gatgcagatg taaaagaagt
    10381 gattatcgca acaaatgcaa caacagaggg agaagcaaca gctatgtatc tctcacgtct
    10441 gatcaaaccc gcaggtatca aagtgactcg tctagcacat ggtctgtctg taggctctga
    10501 tatcgagtat gcagatgaag ttactttatt gaaagctgtc gaaggaagac gtgagttata
    10561 aatttctaat taagtgtttt ttcagataca atataaaagc agcacaagag atgggggcaa
    10621 caatgtgaac ggactattta ttacaatcga ggggcctgat ggtgcaggaa aaaccagcat
    10681 cttgaatgaa ctttaccctc ttttgaaaga agtagcaaaa acagaaatca ttcaaacaag
    10741 agaaccagga ggcattccga ttgctgaaga gatccgagca gtgatccttg atccaaaaaa
    10801 tgatcggatg gatgaacgta cggaagcact gctttatgca gcagcaagaa gacaacatct
    10861 agtcgaaaag gttctgcctg ctctggcaaa agggaaaatc gttttatgcg atagatttgt
    10921 cgacagttct cttgcttatc aaggtgcagg ccgacgcatc ggcgttaccg agattgctcg
    10981 gttgaatgag tttgctacag aaggtactac gccggatttt accctttatc ttgacgtaga
    11041 ttcagacacc ggattaagaa gaatcaagaa aaacagacaa aatcaaatcg accgtttaga
    11101 ttcggaaggt ttggaattcc accagcgtgt acgtcatgct tacttaaagc tggcagaaga
    11161 gaatccggaa agaatccaca aagttgatgc aagaaaaagc tttgaagaag tgctgcaaac
    11221 aagttatcat acaattattg aacagtatcc gcaatttttt gaaaactagg aaggtgaacg
    11281 tttatgaaaa ttattttagc gattatccaa gacaaagaca gcaaccgttt atcaaatgaa
    11341 ttgattgatg caaatatccg tgccacaaaa ctatcatcga caggcggctt ccttaaggct
    11401 ggaaacagta cgtttatcgt gggtattgaa gatgaccgtg tagaagaagc attggaaatc
    11461 atcaaaaaaa cgtgtgaatc aagaaagcaa tttgtatcta cacctgttac actggatatt
    11521 tcgatggatg ggggcgtccc ttatccagta gaagtagaag taggcggtgc cacagtcttc
    11581 gtcttacctg ttgaaggatt ccatcaattt taggaggagc tgagctatga atcaagaaat
    11641 aaacttggct caaatgcaac cgatcgtgta tcagcagctt caacggagtt ttgagcatgg
    11701 gcgtatcgct catgcttatc tttttgaagg ggagaaagga acaggaaaac atgaagtagg
    11761 tatatggctt gcacagcacc tgttctgtac ccaaatgaaa gacaatctgc cttgtggaaa
    11821 atgcaacaat tgtcagcgga tcgaaaagaa agaacatcca gatgttttgg ttattgaacc
    11881 ggaaggacaa accatcaaag tcgatcagat cagacgtttg cagacggaat tcagtcgcag
    11941 cggttacgaa tcaagaaaaa aagttttttt gatcaaagaa gctgaaaaaa tgaatagcag
    12001 tgcggcaaac agtttgttga aattcttgga agagccccct ggagattttt tagcggtgtt
    12061 agaaacagat gcagtaggaa gaattctccc tactattcaa tctcgttgtc aagttctgca
    12121 ttttcaagaa ctttcgaaag aagcactgat ccatcgattg caagaagagc agattccatt
    12181 ggaaaaagca aaacttttgg ctttcttaac aaacagttta ggaaaagcag ttgaaatctc
    12241 tcaagatgaa tggtttaatg atgctaagga cttgatttat caatggttcg tttacttgca
    12301 aaagcaagat actcaagcct ttatctatgt ccaaaaaaaa ttggtaaaat catttaaaga
    12361 aaaaacacaa cagtttacag gattatctgt cttgatgttt tattatcagg aagcacagcg
    12421 tcaagcagta gcaaacggcg aatatggcaa agtgaaacag ataaaccaga taattgaacg
    12481 gatattgatc gcagagcaga aactacgcag caatgtcagt tttcaggctg ttgcggaaca
    12541 gttcgttttg catacgattt tttagcttgc cgcaaaggat gatgatcaag gaggatggaa
    12601 atggtagaag tagtaggtgt ccgatataga gatgcaggtc atatctatta ttttgcacca
    12661 ggagactctg aatatgttta tagtgacaag gtattagtcg aatctcagca atccttacat
    12721 attgctactg ttgtccttcc taaaaaagag attgatccca atgaccttcc tgaagctatc
    12781 cgtccgatct tgcataaagc gtcacaagct gaattagaaa aagacaaaaa aaacaaaaaa
    12841 gatgcttatg atgcattgaa aatcgcaaaa gaaaaagtgc agttgcgtga gttggaaatg
    12901 aaaatggtca aagccgaata cacatttgat cgaaataaat tgattttttc tttcacagcg
    12961 gacgggcgaa ttgactttag agaattggta aaagatctgg ccgctgtatt tcgtactcgt
    13021 attgagcttc gacaaattgg tgtccgcgat gaagcaaaga taataggcgg aattggtcca
    13081 tgtgggcgtc cattatgctg ttcttctttt ttaggcgatt tcgtgcctgt atcaatcaaa
    13141 atggcaaaag atcaaagttt atctttgaat ccaacaaaaa tttccggttt atgcggacgc
    13201 ttgatgtgct gtctgcaata tgagagcgaa gaatacgaga cagcgaaaaa agaactccct
    13261 gactttggaa aagtaattgc tacacctgat ggtaaaggga aagtcgtagg gttgaatctg
    13321 cttagccgta tcgttagtat tcggctgact ggacgggaaa atcctgtcga atatgaatgg
    13381 gatgaattga aagaatttat ccaagtaggt gagatgaatg gataagagat cattatacga
    13441 cggattgaat caattagaat cagagttgag aaatacatta acagaacttg ttgaaatgaa
    13501 gcatgctttg catgagatcg tagaaaaaaa tacaactttg gagatggaaa atcaacgtct
    13561 gcgggaacac ctgcgggaat tgaaccaagt tgcacaagct ccagctgaaa atgtgaagca
    13621 ggaactttct aagtctcgta tgaatcttga aaaaatttat gaagaaggat tccatgtctg
    13681 ctacgattta tatggatcaa gaagggacaa tgacgagcct tgtgcgtttt gtctagaagt
    13741 gatctatcgc gaaagcggtc gatgaccaat aacaagagga gactggagcg gagaaatact
    13801 gctccagtct ttttattaag gagattgtga cagatgtata atcaaaaaag tttcaaggga
    13861 aatccggtat atggggattt atatcttgta ccgacgccaa ttggaaattt agaagatatg
    13921 agctacagaa gtgttcgtat gctgcaagaa tccgatctta tcgcaagtga ggatacgcgt
    13981 aatacacaaa agttattgaa tcattttgaa atccatacac cccaaaaaag tttacatgaa
    14041 cataattata aagaacgtgt ccctcaattg attgctttgt taaaagaagg aaaaacgatt
    14101 gctcaagtca gtgacgcagg aatgccttcc atcagcgatc ctggacatga gctagtttta
    14161 gcctgcataa aagaagggat ttctgtgatc tctatcccag gacctacagc tgggatgacg
    14221 gctttgattg cttcagggct agtgcctcaa ccatttttat tttatggctt ccttccacgt
    14281 aagaaaaaag aacagaaaga aacattgaaa aagctaaaag aacagacagt tacgctgatt
    14341 ttttatgagt caccttatcg gatcactgct acattgacga atttcttaga aatttttgga
    14401 aatcgccaag tcgttttgtg tcgggaattg acaaaggtcc atgaagaata ccttagagga
    14461 agtacggaag agctactcgc ttatatcgaa gaacatccag taaagggtga atgttgtctg
    14521 cttgtagaag ggaatttgtt cgaaatgcca gtgacggaag ttgatgaaga gttgggaacg
    14581 ttaaaggaac aagtagaagc aaagatcgct tcaggcgaaa aaccaaatgc tgctatcaaa
    14641 gcagtagcgc tgaaaaatgg gctgaaaaaa caagaagtct accgtcagta ccatgaatta
    14701 gactgagata gtataagttg tctataatga aagactaatg aggaaatcag ctactaactt
    14761 ttcaaatctc ttgaacgcga acgaatgttc ttgtataatg aataagaggt gaggatgatg
    14821 gagctgcaat ttcctttaca taatgataca tcaagaaaaa tcattcatat tgatatggat
    14881 gcttttttcg cttcggttga agaacgagat catcctgagt ttcgcggaca tccattagta
    14941 attgccagac accccagtga cactggtggg cgtggagtag tgactacagc aaattatgaa
    15001 gcaagaaaat tcggaattca ttcagcaatg agtgcacaaa aagcgtatga gctttgtccg
    15061 caagccatct ttaagcctgg cgaccatcat aaatacagcg atatttccaa acaagtacgg
    15121 gaagtattct atcgttatac agatattgtg gaaccagtta gtatcgatga agcgtatctt
    15181 gatgtgaccg aaaataaaat caattgcaag tcagccatca aaatcgcccg aatgatccaa
    15241 gcagatattt gggagagtgt ccagctaaca tgttctgcgg gagtaagtta caataagttt
    15301 ttggccaaat tagcatcaga ctatcaaaaa cctagaggaa tcacggtagt tgcccctcaa
    15361 gacgctgtgc catttttaaa agctttgcca attgaaaaat ttcatggggt agggaagaaa
    15421 actgtaccta gaatgcatga gctagggatt tatacaggaa aagacctata tgaatgcaca
    15481 gaaatgatgt tgattcgaaa ttttggaaaa atgggctatt ctctttatcg taaagtcaga
    15541 ggaatccatg attctccagt aaacgttaca agagagcgaa aatctgtggg aaaagagcat
    15601 acttatgggc agccgttgca aacggaagaa gcagtgctga ctcagttgcg ccaactagct
    15661 gaaaaagtag aagaagcact gagaagagtc caaaaacatg ggaaaacagt agttttgaag
    15721 gtcaggtaca cggactattc tactgtgacc aaaagagtga cattgccaga atatatctat
    15781 aaaaaagaag cactctttta tcaagcaagc cttatatggg aggaaatctt gggagtagaa
    15841 aaaggaatcc gattgttagg gattactttg acgaatctgg acccgatgac ttatgaaaac
    15901 atcgtccttc cattatggga aaatcaagaa atataaaaac agctttgaga gttgaaaccg
    15961 gaaatccgtt ttctctccca aagctgtttt tatatcaagt cttgcttttt gatttgttca
    16021 taggtttctg ttgcatccgt acatttttcc caaatcacaa cttgattgga ttctaaagat
    16081 ttttggacgt caatgatccg ctggttgcta cttccgcgaa attgtagttt taggtcacgt
    16141 ttgcttaatt caaagcgtcc gtcaacaagg atatcgattt gcgaaaggag ttctaattta
    16201 tcttctgaat cttgtagcaa ttcatcaaag gtatagcctg tccatgacca gatatctttt
    16261 gtcgtaccga actcttttcg gatacgtcgt acgacttgca gacaaacatc tgtattcaaa
    16321 aaaggttcgc caccaagtaa agttaatcct tgcacgtaat cgttgcgcaa gtcagcgatg
    16381 atttgttctt ctaattcagg tgtgaatggt ttgccatatc gaaaattttg tgcagctttg
    16441 ttgaagcagc cttcgcaggc aaataagcag ccactaacgt aaagactgtt gcggacacct
    16501 tcaccatcga caaaattaaa cggtttgtag tcagcaatat agttttggct gtagtctttt
    16561 gctaaccatt cttttggttt tggatttctc ataaaaaaac ttctttcatt taaggttcaa
    16621 agatatagct attgaagata aaatctactc gaccacataa ataagaacaa actgacctaa
    16681 atccattgtt caagaaagct tgttcttatt ttgatagagg gcgttcaaag atacaaagcc
    16741 atcgaaagag ggtggtgaaa aaagacttgt catagcctct tattctgaat aaacggtgtt
    16801 caaaagcttg catctgcagt aataagccca actaaacgaa aaatatgagg aactattttt
    16861 gtttagctat tcttattact tgatgcagga cagctttttc acaacctcat ttcatgtgtt
    16921 tcacacgtga ggaaatttct ttatggcggc catgtaccat cggacgagct tgcggatttc
    16981 ctaaatagcc acaagtacgt ttgactacat cacatgtttt tggatcatga tttccacatt
    17041 ctggacattc gaaccctcgt tctgtcggtt tgaaatcacc ttcgaaacca cattgatagc
    17101 aatgatcgat tggcgtattc gtaccaagat acccgatttt gtcatatgca aaatcccata
    17161 cagcttctaa agctttcgga ttttgttgga gcatagggta ttcacagtag tggatgaatc
    17221 cacctgaaca atattttgga taatccttct caaattccaa tttttcaaat ggcgttgggt
    17281 ttttacgcac atcatagtgg aaactgtttg tataatattc tttatctgtg atattttcga
    17341 tgatgccgaa tttttctgta tccaaacggc agaaacggtc agttagactt tcacttggag
    17401 ttgaatagac actgaaatga taaccatatt catcccccca tgcatctgca ttagctttta
    17461 attctttgac gatatcaagt gtgaaagctt ttgcttctgg gtttgtttcc cattcgccac
    17521 cgaagaaagc tgaagcaact tcatacaatc caatgtagcc taacgataca gtagctcgac
    17581 ggtttttgaa taattcattg acagaatcgg tacgtgaaag acgtttcccg aaagcaccat
    17641 acatatataa aattggcgca tttgctggaa tggcttcttt acaacgttct acacgataga
    17701 gaagcgcatc tttcatgatt tctaagcggt cagacagtaa ctgccagaat ttattctgat
    17761 ctccttttga ttcgatcgcg attcttggca aattcaatgt aacaacacct aaattcattc
    17821 gtccgacatt tacttcttga ccattttcgt ctttccaccc ttgcaagaat gaacgacagc
    17881 ccataggtac tttgaaactt ccagttaatt caacgatttt atcgtaattc agaatatcag
    17941 gatacattct tttcgttgca cattccaatg ctagttgttt gatgtcgtaa ttaggatctt
    18001 cagggtttaa gttcacacct tttttcaaag tgaagatcag tttagggaag atagctgtcc
    18061 gcttttcact ccctaatcca ttgattcgaa tctgtaaaat tgctttttga atttctcttt
    18121 caaaccagtt ttctcctagt ccaaatccta atgaagtgaa aggagtttgt ccatttgaag
    18181 taaataaggt attgatttca tattccaaac tttgcatagc atcgaagata tcttttttgg
    18241 tttttgcttt tgcatattct ttttggcgtt ctggcgaatc gatccattct tccgcgtctt
    18301 ttagatgttt tttatagttc aattctgcaa aaggagcaag caattcatct gttcggtcgg
    18361 cagaacagcc accatattga cttgacgcta cattggcaat aatctgtgag atttgtgctg
    18421 tggcggtctg gatagatttt ggcgattcga cttcggcatt gccgattttg aacccattgt
    18481 tcagcatgcc tttgaaatca atcaagcagc aattcgtcat cggtgtataa ggatgataat
    18541 ccaaatcatg gtaatggata tcgccttttt gatgcgcatt tgctatatgt ggtggcaaca
    18601 ttttcagtcc gattgatttt ccaacgatcc cggctgtcag atctcgctgt gtattgaaaa
    18661 cattactgtc tttatttgcg ttttcgttca cgacagtttg gtccttgttg attagctttt
    18721 caatcgaaaa gttgatatct gttgctttac tgcgagcaaa atctcgtttg gtacgataat
    18781 tgatgtactc ttctgctaat tcatattcgt ttttttctag taatatatgt tccacgatgt
    18841 tttgaatctc atagattttg acattttcag caaaccggct tttgatttct tggacaacca
    18901 agtctgcaat catttgaatt ttttcataag taagtggtga taatgagccg tgaattttct
    18961 tttctgcttt gatcagtgct tcattgatct tttgttcatc gaaatcaaca tggcggccat
    19021 cgcgtttgat gactttcatc tgggatgtct ctggctgctg agtaacgaca gttttttctt
    19081 cataatttac tttcattatc ttcaactcct gctaaattgt tcttgtctat cataaaacaa
    19141 aatcggggaa ggagcaacta aatatcggct atatatagta ttcaatgtgc tgtataattg
    19201 gatttctata ctatatgtag tgtatatttt attgtgaaaa aaggtgaaaa cttgtagctg
    19261 gtggatttgt tttttgcata tttaattttg ctgaataaaa taattgaata aaatttttta
    19321 ttcagtccaa gtaaaagtga caaaaacata agagcaaatt attcgtagat atgctactat
    19381 gcaaataaat agaaatgaaa aatgaaagag gaggagaaga attggaaaag atgatcgaag
    19441 tgaaacatct aagtaaaagc tacggcagca aactttctcc cgtcaaagcc ttagataata
    19501 tctcattcac agtagaagaa ggcgagtttg ttgggattat gggcccaagt ggtgcaggaa
    19561 agacaacgct gatgaatatc ttgtccacga tcaatttgcc tacgatgggc agtgtctcga
    19621 tccaaggaaa agaaatcaca aaaatgaaaa acagtgagct cagtgacttt cgtcgaaaaa
    19681 aactaggatt catttttcaa gagttcaatt tgatcgatac cctaaatgca aaagacaata
    19741 tcctattgcc cctagcagtt gaacggatga ctaaagagga aatggaaaag agagtaagac
    19801 atgtagctca attgttgaat atagaagaac tgctcaaacg gtatcccgat gaactttctg
    19861 tcggacagag acaacgaatt gcagctgcta gagcacttgt tgttcagcca caagtgattt
    19921 ttgcagatga accaactggc tctttggact cgaagtcggc tacagaattg ttgaactact
    19981 tgaagaccat gaatcaaaaa gaaaaagcaa cgatactgtt agtcacccat gatccatata
    20041 cagcaagcta ctgcgataga attttattta ttaaagatgg tgtgattttt tctgaagttg
    20101 tcagaagagg gacaagaaga gagttctttg agaaagtaat agacatgcag gcgacgattg
    20161 gcggaggagg aaaaatgaat gctgtataaa ctagccgtta aaagtttttt tcggcagtca
    20221 cgaggctatc ttgtttattt tttcagtttg actttatccg taatgatcta ttattccttc
    20281 agcgcaatga cctacgatca atctttagtc agaagagcaa gtcaagatac ttcgatagat
    20341 ggtggattga gttttggtgg atttatcatt actgttgttt tgcttttttt cgtattcagt
    20401 gccaaccgat ttttcttgaa tagaaggcaa aaggaaatag gcatctatca gctttttggt
    20461 atgaataaac tgcagatatc aggtatatat gtgttagaaa tcatgataat tggattgttc
    20521 gcctgtattt ctgggatatt actcggtatt attttttcca aactcttttc aatgattttg
    20581 gttcgaatga tggatatgga tcttactagt ccatttttta tctccattcc atcagtagta
    20641 gatactttat ttgctttttt catcatcctt ttagcagtat ctgtatattc aatatggaaa
    20701 atatggcgct atccaatgat tcgaagtttt ggagaaaaag aacaagcaga tagttcaaca
    20761 atgcgcttca ggacaaggca ccgattactc gcagtcatcg ggttactact tttaacgagc
    20821 ggatattttg gagctagtca ttttagggaa atcacttcat ggatgatcag ttatcattat
    20881 cttgatacat tagttttctt ttttcctttt ttaatttttt tcgtttgtgt ggtcggtact
    20941 tatttgtttt tctgttatac atttcggtta tttttgatca tattgagtaa atggaagatt
    21001 tattactatg gtatcaattt tttgataatg ggtaacacgc aagttcattt gctcaaaagt
    21061 tggcgtacaa attctctgat tacagtcatt ctcggaattt ctttaatgac aattggcggc
    21121 acggctagta tctctactat tttatcctac caggagaaag tttcctcccc gatggactat
    21181 cagtttgatg ttgagacaga gaaaaaaatc aagccgattc tagcagagga agggcagaag
    21241 ataactaaag aaatcacctt gcattataaa gtcattggga gtatctatag tcaaaatctc
    21301 gaagaaccta tgtttcaaac tgctaatctg atcacggaaa cacaatatca agccttcaga
    21361 aaagttaatc cagatttgcc gaacatttct ttgaaatcac ctggtcatac tgttttgctt
    21421 gatcaaatgc aaactctttt tcggaatatt tcattatatg gatctagtat tggactgcca
    21481 gataatcaag aattgaagat tcaacaaatg cttccaagtg tattaggtga cgaaacaatg
    21541 gtatatctaa gtcctgtact gattgtagat gataccgttt ttgaaaaagc aaaaggtgtg
    21601 gattatcaag tagtcaatgt agatgtatca ggtggaaata atgaaaaaat agatgaacga
    21661 ctgagcaatg acctgtcgat aaagtggctc aatgctgttt attattctta tgatatcgta
    21721 aatggaagtc tacaaggaga aattagcaca aagaaacttc cggatgacca aatgaaggaa
    21781 gcacaattta gccaagaaag ctctcgtttg aatttctctt ccagctattc gaaattgcgt
    21841 gcagttaatc ggcgaatcgg tatcaatatt tttgtcacac tatttgtcgg gatgatcgtg
    21901 attattgcta ctggtagtat tttgacagtc cggcaattag cagaagcaga ggaagaaaaa
    21961 gaaaattatc acctattgac taagctgggg attccacaaa gaaggatcag acggatgatc
    22021 ttcgagcaaa acgcacttac cttctttcca cccatgatat tagggatcac tcacgcagta
    22081 tttgcaatca atgtcttcac tcagtatata aaaactgccg attactggtt ggcttatctt
    22141 gtgagtggct tattgatcct tatctatctt ttgctttatg tcattacgag tcagttatat
    22201 tgtcggatta tcgaggaatg atatacggaa taagacaaga taataaatag aactaaattt
    22261 tgaataccat ttatttggaa taagaggttg tgacacgatt tttgtcacaa cctctttatt
    22321 ttttatattt tttaaaagta cgagaaatta taaagaaaaa agcagtgatt tgatgtgcta
    22381 tttttataaa agctatgtga caaaactgtt agtaaaaaaa cggtttctgt aagctagaca
    22441 catgtttctt tttgtcaaaa ttagttacac tttgtgtgag gttatatgag gagggaatcc
    22501 aatgaacaaa tggcgttcta atccaaaatt gaattattgg ggaaaattca tcattcgaac
    22561 aattttttat agtgcagtac tgctgttcct gatttatctg tatcactata aaaatattca
    22621 aggaggaact tttatctata acgaatttta ggaggaagag tatggaaatc aaaacgatta
    22681 ttgaagcaat cgatgattgg ggaataaatg aacctgatcg agttgcttat caggcggatg
    22741 agacgcatac ttttggtgaa ttgaaacaag catcagacgc acttgcttat tatttagaaa
    22801 aaaaggtaga aggcgatgga ccaattgtcg tttttggcaa tttggagttt gaaatgattg
    22861 tctcatttct aggggttgtc aaatctggcc atgcctattt gccgattgaa gagcatacac
    22921 ccaaggagcg tatcctatcg atctttcggg tggctaaacc atctatggtc atcagtatcg
    22981 gggactggat agaagatatt ccttcggttc ctgtcataac aaaaaaagaa tttcaagaaa
    23041 tcaagaagat accagtagga ttttatccca gaaatagcgt aaaaggtaca gaaaattttt
    23101 atattatttt tacttctggt actactggag aaccaaaagg cgtacaaatc agtcatgata
    23161 atctagtcag ctttgttcaa tggacgatgg aagactttgg gatcaagcct ggtatgcatt
    23221 tcttagccca agcgccattt tcttttgatt tatcggtatt ttctatttat cctgcattag
    23281 tttctggcgg aatgctgaag ccgttaaata aagcagttgt tcaagacttt cgtcaattat
    23341 tcgcgacatt gccagccttg aagttgaatg tgtgggtttc tactccatca tttatggata
    23401 tttgcttgat ggaacctaca ttcaatggag aaaatgtgcc ggaattggac atgtttttat
    23461 tttgcggaga agaattgacg aaaaaaactg cagaaagtct acttgatcga ttcccaaatg
    23521 cacggatata taatacttac ggtccaacag aagcaactgt agctatttca agtattcaga
    23581 ttgaccagca tgtactagat agttatgacc gactaccgat tggctacgta aaaaaagaca
    23641 cgaaagtttc tattgtccaa gaaggtaaac ctgtttcaaa aggagaaacg ggtgaaatca
    23701 ttattgcagg accaagcgta tcaaaaggtt acctgaacaa tcccgaaaag acagctgctg
    23761 cgttcttttg tcaaaatgac ttagcaagtt accatacagg ggatgcaggt agactagatg
    23821 aatatgggct tttattttat gaaggacgta tggatcagca ggtcaaatta catggatacc
    23881 ggattgaact cggggatatc gaacattatc tgctccaaga caaccgaatc aaacaagctg
    23941 tcgtcgtacc aaaataccaa ggaacaaaag tacagcagct ggttgctttt gtcgtattag
    24001 aaaacaaaac gaaacagccg gattttcagt tgactaaatc tatcaaagaa caattactag
    24061 aagttgtaat ggattatatg attcctcaga agttccatta tgtcgatctg ctgcctcaga
    24121 cagttaatgg aaaaatcgat cgaaaaaaat taacagctga ggtgaatcct gaatgatgtt
    24181 acctcatatg atcccttatg cagagccgtt ttattttttt cttttagcca ttgcgcttgt
    24241 accgatcgtt ctatctttat tgatatggga aaaaagatta ccagtttatc aatcagcagt
    24301 gacacttttc tttttatttg tcagctttgg aggtaattat tggcaacagg gagcggcgct
    24361 gattgggtat gttttgtggc agacgatact tgtatggggc tattttgttt atcggcaaag
    24421 gaaaaatcag acaggatggt tttttgccgc cgttatttta gcgataattc cactgttttt
    24481 aacgaaagtg actccttttt ttgaacatgg aaaagcatcg ttacttggtt ttttaggaat
    24541 ctcttattta acctttaaat ctgtacaaat gatcatggaa ataagagatg gcatgatcaa
    24601 aacatataag ccatttcgtt atatccagtt tctgctgttc tttccaacaa tctcttctgg
    24661 accaatcgat cgctatagga gatttgaaaa agatcagctc catccgcctg ttcgtgataa
    24721 atatgttgat cttttgggaa aaggcgtcca aaatattttt gtcggttttt tatataaatt
    24781 cattatcggt tattattttg gtcaagtact gcttcctgta gtgggacgca ttgccttaca
    24841 gcatggtggt atttcatggg cattagtagg ttatatgtac gtgtacagta tgtatttgtt
    24901 ttttgatttt gctggctata gcttatttgc agtcggtaca agctacatga tgggatatca
    24961 gacaccaatc aatttcaata aacctttttt atcatggaat atcaaagaat tctggaatcg
    25021 ttggcatatg acgctgtcct tttggttccg tgattatgta tatatgcgat tgatgttctt
    25081 catgatgaag aaaaaatttt ttaaaagcaa gatcgtaatg tcaaatatcg gttattttgc
    25141 tttgtttctt ctaatgggta tatggcatgg attgacgtgg tactacatcg tatatggact
    25201 gtatcatgct tgtctgatct gtctgacaga tgcatggatc cgatttaaga agaagcataa
    25261 agagcagttg ccttctaaca aatttacgca tggcttagcc gtatttttga catttcaagc
    25321 agtatgtttt agcttcctga ttttttcagg atttttagat aaactttttt ttagctaacg
    25381 aatagtgaca tattaattta tataacaaag gagaaaagga aaatggaaga acaaatttta
    25441 tcaatcttag aagaaataac aggaacagat gaagtaagaa aaaatcgtga tattgatcta
    25501 tttgatgaag ggttgcttga ttctttagca tctgttcaat tattagtcga gctagacgga
    25561 gagctggata tccaagtccc tgtatctgaa ttcgagcgag aggattggtg cacaccgaat
    25621 aaaatcattc aacaagcaac tgcattgaag ggttgagtat gaaaaagaaa ctttttgcta
    25681 ttttcggccc ggttgtttta gctgttggct tactgtttgt gttttttacc tcctcctatc
    25741 aagtagatat ctctaatcca gaaacgatta gacgtgcctc ttcttccatg tcagtgaacg
    25801 tgttgaaagg agatgcgata aaaaattatg cattatctga aaaacaatat attccttttt
    25861 ttggctcaag tgaattaagc agaatcagtc catttcatcc gtctgttctg gcagaaaaat
    25921 accagcgaaa ctatcgtcca tttttgctgg gagcacccgg gacacaatcg ttaagccaat
    25981 atatgatgat gcgttctgca ggagatgcta tgaaaaacaa gaaagtcgtc tttattattt
    26041 ctcctcagtg gttcgttaaa aatggtgtaa aaactgatta ctttaatacg tattattctg
    26101 aacttcaaac atacgactgg ttgttttcaa tgaagaaagt aacgcctgct gatcgctatt
    26161 tagctagaag attattaact ttctcaaaag taaaagagaa tgatacgttg acggcgattc
    26221 tgcaaacgat caaaaaaggg aagctacctt tacctgaatc tttgaatcag ctgcgcagcc
    26281 aatggaacat gctgaagaga gaggatgaag tattcagcaa tattgggttg aaagatcgac
    26341 agcaaaaaat cgatcatgag tcaaaacgat taccaaaaca ataccaagaa acagaattat
    26401 ccattttagc aaatcaaatt ggagaacgag aaacgacgaa taatccattc gggttgaaaa
    26461 acgattttta tacgcatcgt atccgagccc acgagccaga gctcaaacag tcacaaaaaa
    26521 attgggatta tcgtttttcg ccagaatttt ctgattttca gctggtcttg gatcaattag
    26581 caaaaaacca taacgaagta ttgttcatca tcccaccggt taacgagaaa tggagcgatt
    26641 ataccggact atctcaagaa atgttacaag gatttgccaa aaagatcaaa tttcaattaa
    26701 atagccaagg attcaatcga atagctgatt ttgtcaatca agcgggtacc aactatttta
    26761 tggaagacac gatccatcta ggctggaaag gctggttggc tgcagatcaa cagatcagac
    26821 cgtttttaga agaaaatcat attactgcta gtaagtatca tcttgacgat gcttttttta
    26881 gcaaatcttg gcaacatcaa ataccagata aactacaatt aaaataaaga aaacaggcga
    26941 gtgttgctcg cttgttttct ttatttttga atattctgaa taaaagataa aaataccaag
    27001 tttgcctgaa caaaaatcat aagcaatggt ataatcacaa tatgaatata gaaatggggg
    27061 aaaacatttg gatacaaaag aagaacagct acttattgat ttaacttctg caaaaggagt
    27121 acctggaaat gaagaagagg taagagaagt attcagagaa tatgccaaac cattcgctga
    27181 tgatattttt tatgacggtt taggcagtgt gattgccaaa catggtgctg gcggaggccc
    27241 caaggtcttt atttctggac atatggacga agttggtttt atggtaacaa aaataaccga
    27301 aaaaggattc ttggaattcc aaactcttgg aggttggtgg gggcaagtca tgcttgctca
    27361 gcaagttgaa atcaaaacac gtgaagggaa aatcgtccac ggtgtgattg gttccaaacc
    27421 accacatgtt ttaacacctc aagtaagaaa taaaccttat gaaataaagg atatgttcat
    27481 tgatattggc gcatcaagcc aagaagaagc caaagagtgg ggcatacgtc ctggagatat
    27541 ggtcactcca tatattgaat acaaacgaat gaatggcagt aaatatttac tggcaaaagc
    27601 atgggataat cgtatcggga cagctgtttc tctgcgagta ttagagaatc tatctaaaga
    27661 agcacaccca aatgttttat tcgcaggaag cgatgtgcaa gaagaagtag gcttgcgagg
    27721 tgctcgaaca agtacacacc ttgtaaatcc cgatattgct tttgctttag atactggtac
    27781 agcaggagac actccaggaa tgacaccaaa agaagcagat tcgattttag gaaaaggccc
    27841 acagattttg atttttgatg catctatgat cccgcataaa aaattattga attttgttat
    27901 tgatgtagca gaggaaatgg atattccatt ccaatataca gtgatttctg gcggaggaac
    27961 tgatgcagga caaatgcatt tgacaagaga tggtgttccg tctgtagcca ttaccgttcc
    28021 tgttcgttat ctgcattccc atacatcagt gattcatgaa gatgattatt tgaatacagt
    28081 gaaactagtc acagaattag tgaaacggct tgatagtgaa acagttgaaa aaattcggag
    28141 ttatgaataa tgaataatag aatacctatt tctgaatatc ggaaaaaagc acgagtttct
    28201 ttggaaaatc aatggggaat caatacttgg cttgttttct tatctataat atttacgagc
    28261 gtcatccaga acttttttga cggtttcagt ccttttccgg aagaaagcat gcagcagcac
    28321 ttattgagtt ttctagtcga taatttactt ttattcgcat tcaactatgc attgttttac
    28381 attgcgctag tagttgtacg gggtggcaga ccaaaaagta accttttatt ttcagttttc
    28441 caaagaaagt attttggttc gatacttggc atcaatttga tcaattcagt catcaactgg
    28501 ctactcaaca gtttaatctt tctgcccatg tttttaatca gcggagtgag tacttatatg
    28561 cagctgcttt tcagttcaaa tgcggtacct acgggtgtca tgccagaaga ttttcttgat
    28621 atcagctttg ttttatcgat gatagggatg atttttgttt cagcactcat catgagtatt
    28681 gttggcggtt tattccaatt tgctgcatgg acaaagttcg actatcctga tttgtcggtt
    28741 attcaatgtt tgaaatatgg ctggttttta ttgaaagata gaattggtac gtatattttg
    28801 ttgcagctgt cttttatcgg ctggtatttg ctaggagcaa tcgctttgtt catcggtctt
    28861 ttgtgggtca ttgtttatgt gaatgtgtca gttgcagaat tttatgaaca agcacggatt
    28921 gaaaaaggag gtccagacgc ctattttact tagcatgtaa gcagtttatt catcaaaaaa
    28981 ataacttttg tggtaggata aatctttgag ggggaatgta cgatgaaatt aactgtttta
    29041 ggatgcttag gagcgtatcc ttataaaaat caagggacaa caggttattt gcttcaatct
    29101 gacgggttca atttgttgat cgatgcaggt agtgctacgc tgatcaaatt acaagaacac
    29161 cttgatccac ttgatctaga tgctgtgatc cttagccatt atcatcatga tcatattgct
    29221 gaccttggtg tattacagta ttactggcaa cttcatccta agagaaatcc ggagacattg
    29281 ctttcaattt atggacacac aaaagattcc attcactttg aagaattgac gttggaaaat
    29341 gtctcaaaag gacatcctta ttttgaagct gaagaattaa aaattggtcc ttttctgatt
    29401 acttttatgg aaacaatcca tccagtagta tgttacgcca tgcgtatcgt agaagaaaca
    29461 acggggaaag tattcgtatt tacaggtgat tcaggttatt tggaaagttt cgctgatttt
    29521 gcgaaagatg ctgatttgtt tctagcagat acctatcttt ttgaaggaaa tgaacatcat
    29581 catgcccatt tcacttcaaa agaagctgga gaacttgcga aatcagctaa cgtaaagaaa
    29641 cttgtactta cacacctacc tcagtttggt gatttagaac aattgaaaaa tgaagcgcaa
    29701 aaggcagcag gcaaagggat tgatatagaa cttgctgcag ttgacaaagt gtttaacatt
    29761 taggaggaag aacatgattt ttgtaccaaa tgaaaaccac gacccaagaa tcaatttagc
    29821 aattgaacaa tatttgctgc aagaaatgcc tttagatgaa cctattttgc tcttttatat
    29881 caatgaacct tcaatcatca tcggcagaaa ccaaaatacg cttgaagaaa tcaatcgaga
    29941 ttatgtggaa gaaaatggca ttcatgttgt ccgtcgctta agtggaggcg gagctgtcta
    30001 ccatgatttt ggaaacctga actttagttt tatcatgcct gatgatgggg attcattccg
    30061 tgactttgcg aaagtaacac agccaatcat ccaagcactt catgaattgg gtgttgctgg
    30121 ggcggaattg aaaggccgaa atgatctagt cattgatggc atgaaatttt ctggcaacgc
    30181 gatgtatgct accaacggtc ggatgtttgc tcatggaaca attatgttcg actctgatat
    30241 caatgaagta gtcaatgcat taaaagtaaa aaaagacaaa atcgaatcaa aaggaatcaa
    30301 atcgatccgt tctcgtgtaa cgaatatcaa accatttctt ccagaagaaa agcaaaaaat
    30361 gacaacagaa gaatttcgtc aagaaatctt gttgaaaatt ttcggcgtag agtcagtaga
    30421 agaagtaaaa acgtacgaat tgactgatga agattggaaa aaaatcaatc gaatctctga
    30481 ggaatattat cgaaattggg attggaatta cgggaagtct ccagatttca atttctctcg
    30541 ccaaaaacgt ttttctatcg gttcgattga agtccatttg aatgtctcag aaggaatgat
    30601 caaagaagca aaaatctttg gcgatttctt cggactagga gatatctctg acgtggaaaa
    30661 aaaattggtt ggacagaaat atgataaagc atctctatca aaaatagtca gccaaattga
    30721 tgtgaagaaa tattttggtg cgatagaacc agaagaattg cttaccttac tttattgaaa
    30781 ataaaaaata gcggaatcca catatatgga ttccgctatt ttttatacag gattcgggta
    30841 atttcgagag agttgaacag tgatccgatc aatgaaagta tctttttcct gcgttagtgt
    30901 tcctgtgtaa cgatccgcta cgtctttcat catcattaag tcaaaacgtt cctgatcatc
    30961 taattcttct gtgatgcctc gactaaatag gtttgtatcg taatgtgcac tatgttcgca
    31021 acaaatgacg aaatgttcct cttgttcgtg tatcgacaaa taaatttttc gatacagtgg
    31081 attttcgatg gcatgagttt ctctaaagga atgctctaaa gcgtggatca aaagttgagc
    31141 cagatcgtca tcgttgatag gaagttcttc gggaagatct gcttggaagg ttatctgaat
    31201 attccgtttg gcagctaact tctggtaacg cgctaagata agatttgtca cctggtggtt
    31261 gcaaaaagaa cacagcattt cgaaattttt tgcttcttct aatatctttt ccacatagtt
    31321 tttagctttt gtttggtccg tatgcattaa atcttgcaga ttctccatct cttggacaaa
    31381 ttccaggcga agtgtattta cttcttggat atgctgtcga agctgctcat aatgttgctc
    31441 caacaaatca tttttgattt ttaagaaatc tacttgtctc cgatctttgt cggtatttaa
    31501 gatgtgatcg attagattgt agctgataat aacgattaga atgataatat ataaaatcgt
    31561 agaccaattg atcgttgttt gatagatacc taatgccgtt tggatgtaaa tcaagcaatg
    31621 gataatggct accaagacga tccaaggtgt acaagctaca taaaaacggt tattcttata
    31681 tgcttcgcca atgcatgcaa gtgaagtgac taaagtgctt ataacactag caatattagc
    31741 aatcggcatt aattcaggaa gttctgcagg agtaaaagtt gcataaagca gaacgccgat
    31801 aaaaatcaaa agttggaacg tacaaaaaat tccgtaccat tttttatagt aaaacattcg
    31861 gctccaatag tagcagatga tgaaagctga cgttaagata ataaataaat gagataaaaa
    31921 agaatgaacg aatggttcaa aaagaatacc acttaaaata tcttctgata cagcttcaaa
    31981 tcctaatgcc aaagcaaagc aacttaagag aataagtgag tattcaagac gtttttgttt
    32041 atacatcaag tagatcgtat agaaaaaggt ccctatagcg ataaaaacag cgattatcat
    32101 cgtcaatact tgcggcactg attgtgaata aataaaacta atcaatggtc cagttgttcc
    32161 tagataaact ttcggtggca gtccagcata gttctgataa ggagatgaga ctgtaagggt
    32221 caattttttt ccgatataat ttgctggtaa atctatcaca gccaatgaga gtccgggatt
    32281 gctttcttcc tgataatctt tccgttggta taaaatcttt tcgcctagat atacagtaat
    32341 ccattgatgg tttcctttga ttagtaaatt tgcttcgtcc aacggttttt caagtgtcgt
    32401 ttccataatg aatgtttcgt ctttatccac ggtaggaatc gtacgagtgt acttgctgtc
    32461 aaacttaaaa tcaggatcat cttttgtata gaaaacc
//
LOCUS       DAYYKN010000014        32423 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000014 DAYYKN010000000
VERSION     DAYYKN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.014"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHBB7_000568"
     CDS             complement(99..1229)
                     /locus_tag="ACHBB7_000568"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM9208544.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHBB7_000569"
     CDS             complement(1878..3080)
                     /locus_tag="ACHBB7_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9208545.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHBB7_000570"
     CDS             complement(3140..3343)
                     /locus_tag="ACHBB7_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9208546.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4540)
                     /locus_tag="ACHBB7_000571"
     CDS             complement(3485..4540)
                     /locus_tag="ACHBB7_000571"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9208547.1"
                     /translation="MQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFEELD
                     YAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQFFRK
                     VLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNESNG
                     ETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKIISK
                     THDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSFEKT
                     MSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQEEV
                     EYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHBB7_000572"
     CDS             complement(4610..4717)
                     /locus_tag="ACHBB7_000572"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9208548.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHBB7_000573"
     CDS             4880..5692
                     /locus_tag="ACHBB7_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9208549.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHBB7_000574"
     CDS             complement(6066..6257)
                     /locus_tag="ACHBB7_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208550.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(6502..6732)
                     /locus_tag="ACHBB7_000575"
     CDS             complement(6502..6732)
                     /locus_tag="ACHBB7_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208551.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            complement(6716..6913)
                     /locus_tag="ACHBB7_000576"
     CDS             complement(6716..6913)
                     /locus_tag="ACHBB7_000576"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM9208552.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="ACHBB7_000577"
     CDS             complement(7059..7937)
                     /locus_tag="ACHBB7_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9208553.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="ACHBB7_000578"
     CDS             complement(7976..9094)
                     /locus_tag="ACHBB7_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM9208554.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="ACHBB7_000579"
     CDS             complement(9091..11190)
                     /locus_tag="ACHBB7_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM9208555.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="ACHBB7_000580"
     CDS             complement(11241..12062)
                     /locus_tag="ACHBB7_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9208556.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="ACHBB7_000581"
     CDS             complement(12059..12868)
                     /locus_tag="ACHBB7_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9208557.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="ACHBB7_000582"
     CDS             complement(12889..13383)
                     /locus_tag="ACHBB7_000582"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9208558.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13895)
                     /locus_tag="ACHBB7_000583"
     CDS             complement(13395..13895)
                     /locus_tag="ACHBB7_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9208559.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(13960..16656)
                     /locus_tag="ACHBB7_000584"
     CDS             complement(13960..16656)
                     /locus_tag="ACHBB7_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9208560.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="ACHBB7_000585"
     CDS             complement(17084..18157)
                     /locus_tag="ACHBB7_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9208561.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="ACHBB7_000586"
     CDS             complement(18154..18981)
                     /locus_tag="ACHBB7_000586"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9208562.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="ACHBB7_000587"
     CDS             complement(18982..19788)
                     /locus_tag="ACHBB7_000587"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9208563.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="ACHBB7_000588"
     CDS             complement(19800..20885)
                     /locus_tag="ACHBB7_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9208564.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="ACHBB7_000589"
     CDS             complement(20902..21444)
                     /locus_tag="ACHBB7_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM9208565.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="ACHBB7_000590"
     CDS             21702..22691
                     /locus_tag="ACHBB7_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM9208566.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="ACHBB7_000591"
     CDS             22688..23425
                     /locus_tag="ACHBB7_000591"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM9208567.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="ACHBB7_000592"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="ACHBB7_000592"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM9208568.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="ACHBB7_000593"
     CDS             23989..24621
                     /locus_tag="ACHBB7_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9208569.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="ACHBB7_000594"
     CDS             24706..25353
                     /locus_tag="ACHBB7_000594"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9208570.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="ACHBB7_000595"
     CDS             25358..26341
                     /locus_tag="ACHBB7_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM9208571.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="ACHBB7_000596"
     CDS             complement(26744..28258)
                     /locus_tag="ACHBB7_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM9208572.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="ACHBB7_000597"
     CDS             28396..29202
                     /locus_tag="ACHBB7_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208573.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="ACHBB7_000598"
     CDS             complement(29377..29946)
                     /locus_tag="ACHBB7_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9208574.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32088)
                     /locus_tag="ACHBB7_000599"
                     /pseudo
     CDS             complement(30029..32088)
                     /locus_tag="ACHBB7_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
BASE COUNT         9718 a         6777 c         5509 g        10419 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttaccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gtgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgagccat ttgttgtttt tttctgacaa
    30481 gctttcttct tgtctttttt gttctttttg atctatctgt ttgtttaatg gtaaaacatg
    30541 taatagtgac tgagcatgga gttcttggag tgaatacggg tcatgataac tatacataat
    30601 cgtaaaatca cctgtttgtt tggtcaaata accatcctcc aatggtactt cattgattgt
    30661 aaccgataca tcctttaacg gtacgggttt acctaaggaa tttgttgctg attggatcaa
    30721 ctcttctttg tttatttttt cgtttggatg aacaataaga tcatgacctt ctaatgaacg
    30781 attgtctggt atataaacat aagtaaaggt aatttcccct cctgattgct tcctattatt
    30841 catcagttct gagtgaagta cttcaaggat ctccgaccag tgactttttc tgtctaatcc
    30901 gtttgcttgc atccaatcag aaatagaagt gactttccca ttttgttcat aagtcgataa
    30961 atctatattt ggcactcgat cagaaaattc agcaggtagg cgcaaatcag gaatacgatt
    31021 tacactactc atatgtgttt ctgcttgaag ttctttattt gtttgactat ctataaaatg
    31081 agtgatgaca gtcgatgaag cagcagatgc ttctacataa actacagctg ttgtttctgc
    31141 cacatttgat gttttcgagt ctatataagt taatacaacg ggatattctc ccggagttgt
    31201 agtatctact gtcccgttca ttttgatttc ttctttattt cctgaagttc catcgctttt
    31261 tgttacatat tcaacaagat ctttaggggc gtaactctct cctgttctta agtgaagatc
    31321 ttttcctttg atagctgttt gatcagcacg aacatggatc acagcagatg cagtcattgt
    31381 ttttaaagca attggatcaa aataggacaa ggaaatagta gatgttcctg tattagtcgt
    31441 atccacgata ttcccattgt ttgaaattac tttggacata tctccttgtg ttccatctgc
    31501 gttcttcagc ctttctacca gatcagatat cgaaactttt gtccctgcca gaacatcgat
    31561 tttctttctt tcaaactgtg aataatcttt ttcatataca taagaaactt gtgcgcttcc
    31621 accgtccttt atatctgtca aagcatgatt caaaatatca gtgatctgtt ttaactgatc
    31681 accttttgta ggatcagtca tatcggaaaa gatagaaagc ggcacactgc ttccggtttc
    31741 cgatgtccat aatgttttgt taggaattaa ccagtagtgg tttatcgttg gaggataact
    31801 gactttatta taggaagtca atgcaggaag tagctgtttt ccatttgcat cagtgaaagt
    31861 atgagtaata gtgatagggg aatcagtggt ctcagaaaca gaggtacgta cgaccttgat
    31921 ggtttgtgtc gttgcctgaa cgggtacatg catatagaca cctacagata aactgatcag
    31981 catcgtgcgc atcataaact taacgtagtg tttttcagaa cacgaccgtt ccttatctaa
    32041 acgacaagcg tgtttattta gttgttttga acgtcttttt gaacacattt attcttagtc
    32101 ctccctactc ttgtctaaac atgaagtagt aattttcata agatgatttc attatataaa
    32161 taaaatcaaa agaagatgga ctattaggag agtatttttt ttgatttttt attgtttcaa
    32221 gaaaatcgga ataataactt atgtactttt taattttgtt aagtaatata tctagttata
    32281 tctagttata tctagttacc ttttctttga aaaaaacata aatcagctaa taaatatgtg
    32341 agacaaagct gccatccata aatactagct gattttttca gttaaatgtc tatttttttc
    32401 tctgatgatg gtatttccaa aat
//
LOCUS       DAYYKN010000015        30670 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000015 DAYYKN010000000
VERSION     DAYYKN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.015"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..2136)
                     /gene="glyS"
                     /locus_tag="ACHBB7_000600"
     CDS             complement(61..2136)
                     /gene="glyS"
                     /locus_tag="ACHBB7_000600"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM9208575.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2138..3055)
                     /gene="glyQ"
                     /locus_tag="ACHBB7_000601"
     CDS             complement(2138..3055)
                     /gene="glyQ"
                     /locus_tag="ACHBB7_000601"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM9208576.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3439..4251)
                     /gene="recO"
                     /locus_tag="ACHBB7_000602"
     CDS             complement(3439..4251)
                     /gene="recO"
                     /locus_tag="ACHBB7_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM9208577.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4394..5293)
                     /gene="era"
                     /locus_tag="ACHBB7_000603"
     CDS             complement(4394..5293)
                     /gene="era"
                     /locus_tag="ACHBB7_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM9208578.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5308..5703)
                     /locus_tag="ACHBB7_000604"
     CDS             complement(5308..5703)
                     /locus_tag="ACHBB7_000604"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9208579.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5687..6163)
                     /gene="ybeY"
                     /locus_tag="ACHBB7_000605"
     CDS             complement(5687..6163)
                     /gene="ybeY"
                     /locus_tag="ACHBB7_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM9208580.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6180..8372)
                     /locus_tag="ACHBB7_000606"
     CDS             complement(6180..8372)
                     /locus_tag="ACHBB7_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM9208581.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8396..9367)
                     /locus_tag="ACHBB7_000607"
     CDS             complement(8396..9367)
                     /locus_tag="ACHBB7_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM9208582.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10073..11452
                     /gene="brnQ"
                     /locus_tag="ACHBB7_000608"
     CDS             10073..11452
                     /gene="brnQ"
                     /locus_tag="ACHBB7_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM9208583.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11608..12054)
                     /locus_tag="ACHBB7_000609"
     CDS             complement(11608..12054)
                     /locus_tag="ACHBB7_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM9208584.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12081..12257)
                     /gene="rpsU"
                     /locus_tag="ACHBB7_000610"
     CDS             complement(12081..12257)
                     /gene="rpsU"
                     /locus_tag="ACHBB7_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM9208585.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12419..12847)
                     /locus_tag="ACHBB7_000611"
     CDS             complement(12419..12847)
                     /locus_tag="ACHBB7_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9208586.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12939..13799
                     /locus_tag="ACHBB7_000612"
     CDS             12939..13799
                     /locus_tag="ACHBB7_000612"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM9208587.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(13883..14233)
                     /locus_tag="ACHBB7_000613"
     CDS             complement(13883..14233)
                     /locus_tag="ACHBB7_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM9208588.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14249..15976)
                     /locus_tag="ACHBB7_000614"
     CDS             complement(14249..15976)
                     /locus_tag="ACHBB7_000614"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM9208589.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16189..17115)
                     /gene="trxB"
                     /locus_tag="ACHBB7_000615"
     CDS             complement(16189..17115)
                     /gene="trxB"
                     /locus_tag="ACHBB7_000615"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM9208590.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17260..18693)
                     /locus_tag="ACHBB7_000616"
     CDS             complement(17260..18693)
                     /locus_tag="ACHBB7_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM9208591.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18693..20096)
                     /locus_tag="ACHBB7_000617"
     CDS             complement(18693..20096)
                     /locus_tag="ACHBB7_000617"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM9208592.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20326..21228
                     /locus_tag="ACHBB7_000618"
     CDS             20326..21228
                     /locus_tag="ACHBB7_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9208593.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21269..23155)
                     /locus_tag="ACHBB7_000619"
     CDS             complement(21269..23155)
                     /locus_tag="ACHBB7_000619"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9208594.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23252..24103)
                     /gene="licT"
                     /locus_tag="ACHBB7_000620"
     CDS             complement(23252..24103)
                     /gene="licT"
                     /locus_tag="ACHBB7_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM9208595.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(24561..26345)
                     /locus_tag="ACHBB7_000621"
     CDS             complement(24561..26345)
                     /locus_tag="ACHBB7_000621"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9208596.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26345..28096)
                     /locus_tag="ACHBB7_000622"
     CDS             complement(26345..28096)
                     /locus_tag="ACHBB7_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM9208597.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(28238..28462)
                     /locus_tag="ACHBB7_000623"
     CDS             complement(28238..28462)
                     /locus_tag="ACHBB7_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM9208598.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28713..30299
                     /locus_tag="ACHBB7_000624"
     CDS             28713..30299
                     /locus_tag="ACHBB7_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM9208599.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8531 a         6597 c         5486 g        10056 t
ORIGIN      
        1 aataaaccag agaaatagcc tattttgtgc ctgttctctg gtttatttac atattagaaa
       61 ttattttgtg atgatttgat ctaaacttgc tagtgacaaa gccatggcag caatttgact
      121 taattgcgtt aatcggttct tacgtactgc tggttcttct gccatgacca tcgtctgatc
      181 aaaataattt tcgatcagtg ggcgaaggct tacaagctga tcataatttt cgttgatcgt
      241 ttggtcagca aagcttgttt tcattgcctc tactgctttg tgcagttctt tctccgcttc
      301 gttttcgaac agttcagggt ctacttctga ttgtttttca agctccgctt ttttcgctaa
      361 gttcacgaca cgtgtcaaag cttccatcga tggtttgaag tcttcatctg caagatgctg
      421 cttgaacaat tgtgctgctg caaatacttt tgtcagatct ttttgttctg cattcaaaac
      481 agcttcgatg acatcatgac ggatattctt cgttgtcaat aattggcgca gacggccttt
      541 gataaagtcg atcacctgtt gttgtcccgc tgacaagcca atgccaaaac gatccgtatc
      601 tttactgata acttcactaa tcgtttcttg cagaacagac aaaggaaagg cccatttttt
      661 cgattcgatg attcgaacga caccatatgc ttgacggcgc aaagcatatg gatcgtttga
      721 gccacttggg atcatcccaa ctgcaaagaa agaaaagata ctatctaatt tatcagcaat
      781 cgctaaaaca gaacctactg tagtctcagg taaatctcct tcactggata taggcatgta
      841 atgttcacgg atcgctgttg ctacggctgg tttctcgcct ttcagcagag catatttttc
      901 tcccatgatc ccttgtagtt cagggaattc tcctaccatg ttggttacta gatcaaattt
      961 atagatttca gcagcgcgtt gcagatccat caattcttcc tcagatagtc ctacttgttc
     1021 gcctatgatg cgagcgatga cttgtacccg ttgcattttc tcatagatgc taccgatttt
     1081 ttcatggaaa gtaacagttt ttaatttttc aacgtattgg tcgattgaag ctttcttgtc
     1141 ttcattataa aagaattccg catcttctaa acgagccgtc aatacttttt gattgcctcg
     1201 taccacattt tccaatttta cccgatcgcc attacgagca gaaataaaat gaggaagcaa
     1261 cataccttga tcgttccgta cttcgaaata acgttgatgt tctttcatag aagtgactag
     1321 tacttcttca ggaacagata gatatttttc ttcgaagtcg ccagaaaaga cagttggata
     1381 ttcgactaaa ttgttgactt cttctagtaa atcttcatct agatcaatga tccagtgatt
     1441 tttttcggct aattcgtttg cttgtgtgat gatcatttct tttctttttt gcggtgaagc
     1501 aataacaaat tgttcattca atttttcttc gtattctttt gcatgcgtaa atgtcacatc
     1561 ttcgcctaag aaacgatgac ctcttgaagc tctgtctgtt ttgacatcca agatctcaaa
     1621 cgggattatt tcgtcatcta acaatgccac gatccagtga atagggcgaa tatattcaaa
     1681 atcgtattct gcccagtgca tcgttactgg gaaagtgagg cttttaatga catctgctaa
     1741 acctgtcaat acttcttttg cttctttgcc gtgagtgtat ttcgttacat agacatattc
     1801 cacgcctttt aattctctaa aagtgatagc atctgtcgtc agaccttgtc cgcggacaaa
     1861 tccttcagct gctttcgtcc agtttccatc tgcgtccaaa gcaatttttt tggctggtcc
     1921 tttgacttct tcttcgctgt ctgcttgacg ttctggaatg tttgttactt tgattgctaa
     1981 gcggcgcggt gtagaaaacg tttcgatcgt atcataagtc aattggtttt catctaaaaa
     2041 ttttgctgtt ttttcttcta attgtttcat gcttggtgtg acaacatgag caggcatttc
     2101 ttccagcccg atttcaagta ataagtcttt tgccatctta ttcagcctcc ttcatgtttt
     2161 ctggatttgc tgtgttttta ttcaataaag ggaatcctaa tttttcgcgt tctgccacaa
     2221 agattttggc tacagcacga gccatgttgc ggatacggga aagataacct gctcgttctg
     2281 ttacggatac tgcaccgcga gcatctaata aattgaatgt atggctgcat ttcaaaatat
     2341 aatcatacgc tggatgaaca agattttcat cgatacagcg tttagcttct ttttcaaaac
     2401 ggtcaaagtt gcttagtagc aagtcttggt cacttacttc aaaagaatat ttggaatgtt
     2461 catattctgg ttgtttgaag atctcgccat atttcacacc atcagcccat tccagatcat
     2521 agacactttc aacttcttga atatatgaag caagacgttc tagtccataa gtgatctcag
     2581 aagtaactgg tttacaaggt agtcctccga cttgctggaa gtacgtgaat tgtgtgatct
     2641 ccatcccatc aagccatact tcccaaccca caccggcaca ccccatcgat gggttctccc
     2701 agttgtcttc aacaaaacga atatcgtgtt ctaatggatc aattcctaaa agacgcaagc
     2761 tttctaaata aagctcttgg atattttcag gtgaaggttt cattacgact tgaaattgat
     2821 gatgctgata caaacggttt gggttttctc cataacgccc gtcagctggt cggcgggatg
     2881 gctctacata agcagcattc catggttccg gtccgattgc ccgtaagaat gtatatgggc
     2941 tcatcgtacc tgctcctttt tctgtgtcat aagcttgcat cagcatgcag ccgtttgatg
     3001 accaaaattt ttgtaatgtt aaaatcatat cttggacggt caatttttta ctcatctgtt
     3061 ttgccccttt caatttttaa gcgttgctgt caataagaat acaggaaaga aaacgacaaa
     3121 aagtccctat gtctgattac agacataggg acgaataatt tcgcggttcc accctacttc
     3181 cgaataaaga ttcggcagct tcatttatcg tactcgcaag tgcctcttca aaaatcgttt
     3241 gggtccggct tccactctcc cgaactcgct aaaccacgtg ttttttactg atcttggtca
     3301 atgtacgtat tcattttgca gttttcttgt ttatataata gatcctctca gtgaacgatc
     3361 tatttaatct acggttaata atagacggaa gtccctgtat ttgtcaaata gttttgattt
     3421 ttttgttgta tctttcattt attcttcttt ttcaggtatc tctttttcag gcatattttt
     3481 ctctggcgtc tttaatacgt gtgcccaaga cttcatttga tcaatgaact ttttgctttt
     3541 tagatgaata ccgacatatt catcatacaa ctggtcaatg aggtcacgga ttgcctgctt
     3601 cgtttcttct ttcaagtgga ttccactgat cttatcataa gaaatagccg aaaacattcg
     3661 aacgaaataa acagcgcgag gatctgcatg ataacggtgt tcgtccattg gccagtgttt
     3721 ctcgcatatc acaccgccag atttggaaga atagtcaaat tttccttttg tttccccgca
     3781 tacgacacag tgcgtccaaa ttggcgcgat tccgaagcgt tgcagtattt gaacttcaaa
     3841 gatattcgtg atgatttctg catcatctcc ttgatccatc cgctgcagag cttgttgcgt
     3901 aaattggaat aaatgcgggt catattgatg atcttcgatt gctgcatcta ctagatttag
     3961 caaataggta ccataaccgc tcaaaaagat atcttcttgg atgttccgaa acgcatggac
     4021 gtctttgaca ctgcttaaaa acgacaaacc ttcacttcga aaattaccga tatagactgc
     4081 ttcggtaaag gggagcaaag ccggagctaa tggctgattt tgccgatgga ttcctttgat
     4141 ataaaacatc attttgccag aagattccgt aaagattttg actaatttat ctttttcttt
     4201 gtaattacgg ctagataaaa tcaagccttt cgattctgca agctggctca tattctccct
     4261 cctaaaaaat aaatctggaa aagcagattc tgccgatcca gattatttat gtttcgatct
     4321 tccgccgttt ttctcaacgt gcggattttt atccagttta tttctatact taacctataa
     4381 gaagttcaga atatcagtat tcgtcttttc ggtagccaaa gtcttgcaga tagacttttt
     4441 tgtctcgcca gtctttttgg actttgaccc aaagttctag aaatactttg tctcctaaaa
     4501 gattttcgat atcctttctt gctttcgttc ctacttgctt cagcattttt ccgcctttgc
     4561 cgataatgat tcctttttgg ctatctcttt cgacgatgat cgtggcctgg atatggattt
     4621 tatcattctc gtttcgcttc atcgaatcaa caacgactgc gacagaatga ggcacttcgt
     4681 ctcgtgttaa aaacaagact ttctcacgga tcaattcaga aacgataaag tattcaggat
     4741 gatcagtcac ttgatcatct ggaaaatatt gtgggccctc tggcatttca ctgactaata
     4801 catccattaa gcgttcgaca ttgtttcctt ctgtagcaga gatcggaatc acttgtgcaa
     4861 aaggcatctg cgtcgaataa tcttcgatga ttcccagcaa ctcgtctgga tggatcttat
     4921 cgattttatt gataatcaga taaactggag aatgcacgtt ttttagacgt tcaataataa
     4981 aatcgtctcc ccggccgcgt ttttgatcag cactgatcat aaataagact gcatccactt
     5041 ctttcaacgc actgtaagct gcttctacca taaaatctcc tagacgatgc tttggtttat
     5101 ggattcctgg tgtatcgata aaaatcagct gagcttctgg tgtagtatat actccttgga
     5161 ttttatttct agttgtctgt gccttgtcac tcataatggc aattttttga cccacgattc
     5221 ggttcaataa tgtggatttt cctacattcg ggcggccgac gattgcgaca aaccctgatt
     5281 tatgttctgg catatacgtt ctccttttta attaaacaac aaatgatata ttttagggac
     5341 aaataaaatc aacccaatga tgacagcaaa acaagcggtc actaaaactg ctccagctgc
     5401 catatctttg atttttttcc caatcggatg aaaatgaaaa tccgtaaaca tgtccactac
     5461 attttcaaaa acagtattga tgatctctac tatccaaacg agaaaaacag ctaaaagcag
     5521 ccataaccac tctatccgat caagctggaa aatcacgccc ataaataaag caaggatacc
     5581 gaacgcaaca tgtttcctca tattgcgttc ttcctcaaaa acggttttga ctccttggac
     5641 agcaaattcc acagacatca ggaaatgttt gtttttctct acgtttttat cttttgagtc
     5701 cataggcatc caagatctct ttctgtaaac caaacatttc tttctcatct tctggtgtca
     5761 tatggtcata cccgttgata tgcaagaatc catgtaccgc taaaaagcca agttcccgat
     5821 catatgaatg gccgtaagct tctgcttgtt cagcagcacg ttcaatagaa atcatgatgt
     5881 ctcctagtac acgaggcaaa ggattctctc catcttcaaa aatcactggg atttcgtctt
     5941 cgccttcttc ttccagagca aaactgatga cgtctgtcgg tgcatctttt ccacggtaat
     6001 cacgattgat tacttggatt gccgcattgt ccatgaaagt aacagacatt tctgtttcct
     6061 ctggcagctt taaataagat gctgcaaatt gcagcaagtc atcgatttct ttcattttct
     6121 cttctgagac ttggtttgtt tcatccataa aggtaatatc cattatacat cgcttctttc
     6181 tattcttctt gttcttcctt tatcttctca acttctgttt tcattttagg atatttgatc
     6241 cgagaatgga aggtgctact taaaccgctg atcaatgatt tttccacttt tcggatttct
     6301 ttcaaggtca gtccactatc gtctaattga ccatcggaaa tacgctcttc gataagatta
     6361 tggacaaaac tttcgatttt ttcgctcgtc ggatgatcca ttgcccgtac tgcagcctca
     6421 caactgtcag caatattgac gatccctgct tcacgacttt gcggttttgg tccaggataa
     6481 cggaattgct cttctgttac ttccggattg cgttctttcg ctttgatgta gaaataacgc
     6541 attagcgtgg ttccatgatg ctgtttgcaa atatcgatca ccatttgcgg catattataa
     6601 tcttccaata ttttggctcc atctgtcaca tgtccaaaga taatatgttt actgtcttct
     6661 ggcaataaga aattgtgcgg gttttccgct cctgaaggca agttctcaac gaaaaagcta
     6721 gcatgtttga tcttgccgat atcatgataa tagcaagcta ctcgagttag caaagaacgt
     6781 ccgccgattt ctgctacagc gttagcactt agattagcta ccatcatact atgatgataa
     6841 gtacctggcg cttcttccag caactgtttc aataaaggat gatttggatt gctgagttca
     6901 tttaatacaa ttacactgtc atcattgacc atcaattcaa tatatgggtg tagaccgatc
     6961 gtcagcaaga atgagaagag tgcaccagtg aacccgcaga caagcattaa ccaagtcgtt
     7021 gcatcaccaa agctcattcc ttgatagacg atcaaaatga catccatcat cacaggaaaa
     7081 ataatgaccc acatggcagc agataatcct tgttctgaga tccgtttgcg tctgacaatc
     7141 gttgccatga gcccagagaa aacataagac atcaagataa tcgttagaaa gttcgtgcca
     7201 atcgaatcat aaaaaatgaa aagtgcagat actgcttgga aaataccagc catgatccct
     7261 gctcttcggt tcaagaaaaa gcttaaaaca agtggtgcaa aagcagcggg ataaaacagc
     7321 gggatataag tagcttgttc cgtttggaat aattggaaaa atttcatcag cactatgctg
     7381 actgacatag ctgtcacata gaaaagcacg tattttgttc tttctcctgc attttggaat
     7441 tgaagtgaat tataaatcag tacgccgatt tgaagcaaaa cagctaaaac catggcaatg
     7501 agtgggaaaa tcgaagtcgt ttgattcgtc aatcctagta attccaattt tttcatcgct
     7561 gaagcatcaa tttgatttcc ttcgcgaaca atgatttctc cttggaagat catcactggt
     7621 tgtacagctt ctcgtgcaga ttgttttaat tcttctgtct ttttctcatt caatacatca
     7681 ttgactacaa tgccttgatc cactaagtag cgaatcattt gttgctgctc tgttgtgaca
     7741 ttcaaatact gaatctgatc ttctgcttct tgtttaaaag cttccaattc gctttcacga
     7801 acttgtttac tcatctgttc gtcaatgagc ttcaaacttt catccctaac cgtgtgaatt
     7861 tccgtttcag tcatttcaaa tatcgtcgaa taaaaattgt tcggcagttt ttggtaaaac
     7921 gcaacgtttt ctgcattcac gttttcaaaa tttttcttca gtgtagcaat ccgctcttct
     7981 gtagtaggtt tcgggatatt ttcatcttcc ttcgccttat ctaccttgtc attataagat
     8041 ttgttgatgg tatcattcgt cttatcgatc aattcgaata attgcttgat ccgattgttc
     8101 tggtcatctg ccagatcttt ttgataagta tattctggag tcactgcttc tgctgccagt
     8161 tttcttcttt gttcggtttc ttccgtgttc tcgatcgttt tattcgcacg gatactctct
     8221 tctgctactt ggccttctct gaaatcattg gccttttgtt tgacactact aaatgtaacg
     8281 ccgcagacaa tgataaaaaa caagaacaga aggaaaggca atgctgcctt tccgagtttt
     8341 tcttgtagtt tctgcagtgt tttattcaac atgtgcttat cctcctaagc aaagcttatt
     8401 cttgatgaag atccgccttt tcataagctt tgatgatttc ggcaactaca gggtgccgaa
     8461 caacgtcact tgcttcaaaa ttaacaaaat cgattttttt gatttgcttt agtgttcgtt
     8521 ctgcatgaac aagaccactt gtcgtccctc gaggcaagtc tatctgactt gtatccccat
     8581 tgacaatcat ttttgattga taaccgagac gggtcaagaa cattttcatc tgagcgactg
     8641 tcgtgttttg tgcttcgtcc aagataacga atgcatcatc caatgtccgc ccccgcatgt
     8701 aagctaatgg tgcgatttcg attacgcctc gttccatcaa tcggttcgta tgatccaatc
     8761 caaaaatttg gtataaagca tcataaacag gccgcagata aggatccact ttttctttca
     8821 gatccccggg tagaaagcct aggttttccc ctgcctctac agcgggccgc gtcaaaatga
     8881 ttttttgcac ttcgcctttt tttagggcag caattgctaa tactacggca agaaacgttt
     8941 tccctgttcc ggctggccct acaccaaata cgatgtcatg tttggctacc gattcaacgt
     9001 atttcttttg accgctgttt tttacccgta taggttttcc attacggtct ttgacgatct
     9061 cttcctcata catctcaaca aaataatcta atgtaccttt ttgccccatt ttcaatgccg
     9121 tcacgacatc tggtgtactt acgtggattc ctcgtttgat caattcctgt aatgtccgaa
     9181 tgactgaggc agccaaggaa atctgggatt gttcaccaat gatctgaatg atttctccac
     9241 gtgtatggat ggttgtttcc gttgtttcct cgatcagttt tatatgcttg tcgtgtgcac
     9301 ctaaaagcat gctgacatca tcagcagctg tcaatctaat ctctaaagaa ctagactctt
     9361 ccgtcaaaaa tagacatccc ctttttttcg attcattctt tcctatttta ccatactcag
     9421 aagaaactat atagcaaata agaaaaggtt gtaaaagagt cgtttaacca tacgtaccaa
     9481 gaaacgttaa aaaatagctg tttccattcg ctaaaggtct cgttttgata aaaagaatct
     9541 gagaatgtaa gttatatgaa agtggagaac aaaatattcc gtttcattca gaggtattct
     9601 ctaggtattt ttcttgtttc aaaaaaagaa aacaaaatag ttaaaatatt cttgcatttc
     9661 aaaaaaaaca ggtgtattat tacgtcaaca aataaacata cagcaaagcg tcgaggaaaa
     9721 cagtaggcta ttcaacgatt tattcccagt gagtcatgta agtgagaaat gacaataaat
     9781 cgaatagacg aacatcattt ccgagtcaat gtcgaaaacg tagatcattg tggctgcctc
     9841 cattatcagg ctagcggata actttatcaa aaagcgtccg tgaatgaaga agctttttta
     9901 agcgaaaaaa ggtggtaccg ttctagaatc tagagccctt ttcccaagtg gaaaagggct
     9961 tttttttata ttttaaggtg gtggttttca tggaatctga tattagtggc attactaatt
    10021 gctgcaacgt ttatatcgtc aatcaaaatg ataattattg gaggaaaaat ttatggaaga
    10081 aacaaaaact tattcatgga aacaactatt gatggtcagt tcacttatct tcgggatgtt
    10141 tttcgggtca ggtaatctaa tctttcctgt ccacttaggt caaatggctg gcgcaaactg
    10201 ggtcagtgct ggcgtaggat ttgccatctc aggcgctctt ttcccattac tagctatctt
    10261 agctgtagta gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg
    10321 gtatgctgcc ttgttcttaa tccttgttca tttgacgatc ggaccatttt tcggtacacc
    10381 acggacagct gctactgctt ttgaaatggc tgctaaacca tttttgcctg aaaaatataa
    10441 ttcattaggt atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt
    10501 ccatcccaca cggttggtca aatacgtcgg aaagtatcta aactccttgt ttcttgcatt
    10561 attagcaatc atttttgtcg tttctttatt ccacccaatg ggaaatctaa atcaatctgc
    10621 tgcagatact taccaaacaa acgctttaac aaatggtgtc ctagaaggtt ataacacggt
    10681 tgatgcagta gccttgcttg ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg
    10741 tttcaaagat aaaaaagtca cagaattaac agcaaaagct ggtagcctga gcgttgcgct
    10801 agaagtcatg atctattttg gtcttgttct tctcggtgcg atgagtttga ataagatgtc
    10861 tttatcagaa aatggcggaa ctgcactttc tcagatcact aatttttatt taggtaactt
    10921 cggcgctagc ttcttaggag tgatggtcac attaggtgtc ttcactaccg ctatgggact
    10981 cgttgtttct tttgcacaag atttccataa actgttccct aaagtcagtt atatgacatg
    11041 gctgcggtta acgactttcg tctcttttgt cgttgcaaat gctggtttgg acaatatcat
    11101 ccaatggtct ttaccagtat tgatgctttt gtatccattg tcgcttgcgt tgattttgct
    11161 ttccttaaca gcaaaatttt tccaaaagac accatttgtc tatcaagtaa cgatgttgtt
    11221 tgctgctgtt cccgccgtat tggatatgct ggcaaattcg cctgctttag tgagtcaaca
    11281 aagagttgta gcaagcatgc tcgaatttta tcatcatcac gttccttttg ctgcattggg
    11341 acttggctgg atggtcccaa cgttacttgg gtatgctggc agcttactct tctattacgc
    11401 ctatcgccta tcagggtata aacaagaagc aaatgaacta ccagaagaat aaccagatca
    11461 aacacaattt aacataagaa atattaaaca ggaaaaaaac agctagatga aagaatccta
    11521 ttctttcgtc tagctatttt ttagatctct tttttacgag cacaaaaaaa ctgcggacaa
    11581 tgattgtccg cagtttaaat gataaaatta agattgtaat agttctttca caatggcatt
    11641 tacttggttt ccatctgctt ttcctttgac tttaggcatg atcgcaccca tgactttgcc
    11701 aaattctttc attgatgaag caccagtttg cgtgattgct tcttgaacaa gttgtcgaat
    11761 ttcttcgtcg gaaagttgtt ccggcatgta tttttcgact atcacaattt cactttttac
    11821 tttttcagct aaatcgtcac gtcctgcttc ttcaaactca tgcaaagaat cacgtcgctg
    11881 tttcatttcg cgagacaaca ccgttaattc ttcttcttct gtcaagtcgt gaccttcttt
    11941 aatctgctcg ttttgtatag atgacttgat cattcggatg acttgtaaag attccttatc
    12001 tttcgctttc atcgctgttt tcatgtcatc gtttaatcta cttagtagtg acacactacc
    12061 aactccaatt catcacagaa ctagaatttt ttgcgttttc tagctgcttc agattttttc
    12121 ttgcgtctta cacttggttt ttcgtagaat tcacgtttac gagattcttg taaagtaccc
    12181 gcttttgaca cggaacgttt gaagcgacga agagcatcgt caagtgattc gtttttgcga
    12241 acgactgttt ttgacatgtt aattccctcc ctccgagctt aaaaagtaac cgtaattgtt
    12301 attgaaatat atctcaaaaa acaagactgt cctgtaaata ttatatctaa tgctgttttg
    12361 accgtcaagg aaatcttgct tttttttaaa attatcaaat gttattttat ttaaatcctt
    12421 attgacaatt ttcacatctt ccgaaaatct cgaatctatg cccttcgatc gtacatccac
    12481 cgagctgttc ttcgaaataa ttcattggac acatatggat ttctttcgtt gttccgcaca
    12541 ccgtacagat aaagtgatgg tgatggccga tttccccttg acagcaatga aaacgaaact
    12601 tcatctctcc atttaaatcg gtttcttcca gtaaatcgat ttctgcaaaa tcatggagat
    12661 ttcgataaat cgtatcgtaa cttactcctt tgaattcctg attcatatag tcaaaaactt
    12721 ctttagcgga tacgtagcga ttgcgcttca ctaaataagt taacaatgct tctctttttt
    12781 tagtatattt aaaaccgtta tttttcaatg tttctagtga ttgttcgatc aacgtctgtt
    12841 ttttcatgac ttattcctcc aaatcaaaat gattacgaag tacacattct agagtatggt
    12901 ataataaaca taaaaaaaat gcaagtgagg ttttatccat gtcttccgat tctacagaaa
    12961 tcgttacttt ttttgtcatc tccgactctg ctggagaaac tgcgacaaaa ctagcgcaag
    13021 ccactatggc ccaatatcct agcgttgagt tcaatttatt ccgcagaaca tttgtgacag
    13081 atgagaaaac attgatgcag gcactgcaag atgcactaga agaaaaagct cttgtcctgc
    13141 acacattgat caatcaagaa ttgatcgaga tgacgcgttc tttttgtgaa gaacatcaat
    13201 tatttagttt tgatgtgatg actccgccga tatcagaaat cgagcgctta actggtatca
    13261 agcccattcg gcaacctggc gcccttcatt tattgaatga gaactatttc aaacggatca
    13321 aagcaatgga atttgctgta aaatacgatg acgggaaaga tcctcgtggg tttctagaag
    13381 cagatgtcgt tttgctaggc gtatcccgaa cttccaaaac gcctttgagc ttgtttttag
    13441 ctaacaaaaa tttaaaagtt gccaatctac ctttgatccc acaagcacat attcccaaac
    13501 agctttggga gatcgatcct aaaaaaatcg tgggactgac gaataaccca gatattttaa
    13561 acaatatccg taaagaacgg atgcgctctt acggattaaa tcctgatact gcttattccg
    13621 atatcgaaaa aatacgagct gaactcgatt ttgcgaatga tctttatgaa aaattgggct
    13681 gcgtagtaat caatgtcgct tctttatcta ttgaagaaac agcttcgctt attttgaatg
    13741 ctttagacct agaagaccat agttattatg gaactgaaac caccgaagaa acagactaac
    13801 aatttttctt taaaaaaact ggactgtgtc agcgaagaag attcgttgac acagtccagt
    13861 ttgtctctat ttgcacctat cgttattgat gttccacatg tttgagtgtc tggttcaaaa
    13921 tatccagtac gtgagaatca tcaagactat acaaaatcgc ttttccctca cgatgggact
    13981 tgacgacatg gttttctctt aataaacgca gttgatgcga tacagcagat tgttccattt
    14041 ccactgcttg gatgattgct gtcacatttt gttccccgtc tttcagatac agcagaattt
    14101 tgagacgagt ggggtcactc aacattttga atagttggct gactttctct acttttgttt
    14161 tttgcgtgtc tggcaatcca ttcacctcca acttgaaaaa agactgactt ctaaagttta
    14221 ccactttttc catcagtctc aagttttttt attcttctgt catcttttgg ataatcgttt
    14281 cataagcggc tactttttca gctgcgtctt ctgaagagtt tccttttaca ccgatataaa
    14341 atttgatttt cggctcagtt cctgaaggac ggatagcgat ccagccattt tcttccagcg
    14401 tatacttcaa tacgtcagat ggcggtgtcg ttagtttttc tacgtttcct tcacggtctg
    14461 ttctcgtcaa ctctttgaag tcttctgttt ggatgacttt tgctccggca aattctgttg
    14521 gtgcttcttc acggaatttt tccatcaaag cagagatttt cgcagaacct tcttgtccgc
    14581 tcattgtcac agaaatcgtt ttttctgcgt agtagccata ggtttcaaaa atcgtttgca
    14641 agccatcata caacgttttg ccttgttttt tatagtaagc tgctacttct gctaataaga
    14701 gcaatgcttg gatcgcgtct ttgtcgcgaa cgaaaggttt aactaaataa ccgtaacttt
    14761 cttcgaatcc gaacataaaa gtatgggaat gatcttcttc aaattgttga attttttcag
    14821 caatgaattt gaatcctgtc aatacattga acatggtagc cccatagtgt tccgcaattg
    14881 ctgtaggtaa ttcgctagaa acgatcgatt tcaataccgc tgcgttttca ggtaaagtgc
    14941 ctcgcgattg gtgtgcttcc aaaatatatt gagcaaacag tgctccgatt tggtttccgg
    15001 tcaatacttc ataggagcca tttggcaaac gaacggctgc acctaaacgg tctgcatcag
    15061 gatctgttgc aatcagcaaa tcagcgcctt ctttctcgcc aagtcgtatc gcatactcaa
    15121 aagcagagtg ttcttctgga tttggtgatt tgactgtagt gaaatctggg tctgcgacag
    15181 cttgttctgg aactagtacg aattgttcga atccagcttg tttcaacgca cgttcgccca
    15241 acatttttcc tgttccgtga agtggtgtgt acacaagttt caaatctttc cccattgttt
    15301 ccaccaattc atgatcaatc gttactgctt tgatttcttc tagataagcg gcatccactt
    15361 cttctccgat gatcgtgatc aatccgctat gttcagcttc ttcgtcagat aatacttcaa
    15421 cttttaacgg attgctgact tcacggacat atttcgtcaa tgcatctgcg tctgcaggag
    15481 gcatttgtcc cccatcttct ccgtagactt tatatccgtt gtaagctgct ggattgtggg
    15541 atgcggttac catgattccc gcaaacgtct tcaaatgacg gactgcaaaa gaaagctctg
    15601 gtgttggacg tagactttcg aatacataag atgggatatt gtgatgtgct aatgttttgg
    15661 ctgcttccat ggcaaattct ggtgattgat ggcgtgagtc ataagcgata gcgacacctc
    15721 ggcgttttgt ttcttcgcct tgtgtatcca tgaaacgtgc aagtccttct gttgcttgtc
    15781 ttaccgtata gatgttcatt cgattgatcc caggccctaa gattccccgc atgcctgcag
    15841 taccgaattc caacggtgca taaaaagcat cttcaagttt ttcaggatct tcacccagat
    15901 catttaattg tttttttaag ttttcttcta aattttcttc atgtacccat tgttcataca
    15961 cttgttccca agacatgaaa ataacacctc tttctgaaat atggttctta ataagtatag
    16021 cattcactta ttagatatga aagtatgagg accaaaaaac gcttaaatca agaaaggatt
    16081 cttttatcaa gcacggttgc aagtattcgc ataaatcgga ttcagtagat ttcacgcgaa
    16141 aaaaaagagt aaaagaacaa ttaaaccgtt cttccactct ttttaaactt atgcagttgt
    16201 ttcttctaaa gattccaagt atttatacac ttgttgtcca gcgatcccac catcaccaac
    16261 agcagtagtg atttgtcgca aatctttttc acgaacatct ccgatggcaa aaacgcctgg
    16321 tatattcgtc ttcatttcgt tgtctgttgg aatccagcca gcttcgttcg tcaaccctgc
    16381 ttctctaaat ggtgcagtta atggatccag tccaacatag atgaatactc cgtctgctgc
    16441 caaatcaaac tcattgccgg ttttgacatt acgtgctttc acacctgtca ctaccatttc
    16501 attcccaacg atttcgtcaa cgactgtatc ccacataaag gaaatttttt cattcgcaaa
    16561 tgcacggtct tggatgatct tttgtgcacg taattcatct ctacggtgga cgatgactac
    16621 ttcagaagca aattgtgtca aatagatggc ttcttctact gctgagtctc ctcctccgat
    16681 cactactaga cgtttgttac ggaaaaacgc accatcacaa actgcacagt aagaaacgcc
    16741 acgtccagca aattcttcct cgccaggtac gcctaattta cggtggatac agcctgttgc
    16801 gataatgact gtttttcctt cgtaagtttt atcttcacaa ataacttctt tgtggctgcc
    16861 gtgatccttg atatcttgca cgattccata agcattttct gtcccaaatc ttgaaacacc
    16921 ttcatacatc ttataagcta gatcaggacc cattattgaa tcgaaaccag gatagttttc
    16981 gatttctgcc gtattgttca tttggccgcc aggggcgcct tgttcgatca tcaatacgga
    17041 taagtttgaa cgtgaagcat ataaagcagc agtcatccca gcaggacctg cgccaataac
    17101 gattacatca tacatatgta gattctcctt tacttaatca agtaagcata ctttacagcc
    17161 aatgcgaaaa aaagtctatt gttctgcttg cgtttctcga atagctttat tatactcgaa
    17221 aaatccgctg aaaaacaata gacttttata aatcattgat caatcattcg aaagattttc
    17281 tccatttgaa tcaattactt ttttatacca gttgaatgat ttcttcttcg aacgggctaa
    17341 tgtgccattt ccttcgttgt cacgatcgac ataaatgaat ccgtaccttt tcttcatctc
    17401 acctgtacca gctgatacta aatcaataca tccccaagtc gtatagccta atagatcgac
    17461 tccatctaat tctactgcat ctttcattgc ctggatatgt gctgccagat agtcgattcg
    17521 atagtcatct tccacataac cattctcatc gggcgtatcg actgcaccta agccgttttc
    17581 tacaataaat agtggttttt gataacgatc ataaatatca ttcatcgtta ttctcaagcc
    17641 taatggatca atttgccaac cccattcact tgcttccaga tatggatttt tgacagaagc
    17701 aaagatattt cctgcagtct gttccaagag ttcgttgtca gtagtcgaaa ctcgagaaga
    17761 ataataagaa aaggagataa aatcaacagt atgttctttc agcaatgcgg catccccagg
    17821 ttccattttg atctgaatgc catttcgttc catttctttc aatgcataag caggatattc
    17881 tcctcgtgac tggacatcaa tgaagaaata attttctcga tctgattttc tagaagccca
    17941 aacatcttca ggattgcaag tgtatggata atatgctcct gctgccagca tacagcctac
    18001 tttgttctcc ggatctactt catgagcaat cttcgttgcg atggcacttg caaccaactc
    18061 atgatgtgcc gcttgatatt tgatttgttc ttcattttct ccttcttcaa aatacagccc
    18121 tgcgcccatg aatggtgcat gaaggatcat attgatctca ttgaatgtca accaatattt
    18181 gacaagtcct ttatagcgat tgaagatgac tcggcacaga ttttcataaa aaccaacgag
    18241 ttctctggaa cgccacgcac catattcttt gatcaaatgc atcggacaat caaaatgtgt
    18301 aatcgtcaca agcggctcga tcccgtactt ttgacattct ttaaagacat cctcataaaa
    18361 tttcaaacca gattcatttg gctctttttc atcgcccatt ggaaaaatac gactccaagc
    18421 aatggacatc cgataagtct tgaaccccat ttctgcaaat aaagcaatat cttccttata
    18481 atgatgatac atatcgatcg caatttttgc tggataaaaa tgattttcat caaattcaaa
    18541 cattttcttt tttccagtaa tgacaggaaa acggtcttct ccgattggca caacatctac
    18601 gttcgctaac ccacgtccgc cttcatcata accgccctcg cattgattgg ctgctgtagc
    18661 tccgccccat aaaaaatctt ttctgaatcc catcatactt cctccattac atgtgtattg
    18721 cttgataatt ctcttgccag ttcatcttgg acttcgattc cttctgcttt taaccggctg
    18781 attgtttcct tgaattgcgg taaatactgc tgatgtgcaa taaacaactc attcatgatc
    18841 cgttttgctg tctttcctga tcgtataagc ggattgatcg taaacgcttg taaagctgtt
    18901 ccataatcac cagtcacagc tgcttcgatc gtcagcaatt ccatattctt cattacttgc
    18961 aaccagcctc tttcagcagc aggaagggaa ccaaacgcta catttcgtgc gccttgtgcg
    19021 ccaacatagg cagtgacttc tactacacaa tctgccggca gatcaggaac agctccatca
    19081 ttctttgtag ataccacgat ctgtgtttct ttatttccat agatcgaagc aatcgtttca
    19141 caggcagcat ctgaataata agcaccacct cgtttttgca attgttcagg tttatgatcc
    19201 aattctggat ctttgtataa ttcaaacagc tctgcttctg tttctttcac ttgctgcgct
    19261 cttgtaccaa tcgtttgata ttcttctaaa gcatgttcca gcatttcttc ttcacggtag
    19321 taatagcggt ggtagccaca agggatcatg ttcatttgtt ttaattgttc tttgaaaaac
    19381 gggatatcaa agatattttt cggcaatcca ttgtcttcat cgtataaacg atcaatcaat
    19441 tctgccgtgc ggtcatttcc tttattatct gctactttgt gccaatggaa gtgatttaga
    19501 ccggcaaact tgtagatcag ctcttctttt ttcttgccaa gcattgttgg ttctgtcatc
    19561 atcgccatta ctggcacatt gcataatcct atcactttat cccatttgcc atatctgata
    19621 actgcttctg tcaccatccc gcttgggttc gcgaaattga tcagccaagc atttggacat
    19681 aaacgcttca tgtcttcaac gattgccaaa accactggaa cggtacgaaa agctttgaat
    19741 atcccgccag caccatttgt ttcttgccca atcatcccat aataactagg gatccgctcg
    19801 tctttgattc gtgcatctaa ttgaccgaca cgaaactgag tcgtaacaaa atccgcatct
    19861 ttcaatgctt cttcccgatc cagagttgca tagattttga cttcatacgg agaagcatcc
    19921 cacatacgct gagacatctg actgacgatc tcaagctttt cttttccttc ttctacatct
    19981 acgagccaga tttctcgtat cggtaattcc tcatatcttt tgataaatcc ttccatcaac
    20041 tcgggggtgt aactgctgcc tccaccaatc gtagcgattt tgattccttt tgccatgttc
    20101 tcactccttt actttttaca cttttattat cttttttgta aacaaaacaa acaatagaaa
    20161 ttcgtttctt tccagtaaca tttacaaaag ttagtgtttt ctactggatt tttgtaaata
    20221 tcttgtcaaa aaatgtcttt attaagctat ataacataaa tccgtttaca aatcattttc
    20281 aaaaaagtta taatttacgt aaaaaaacaa gaagaaggta tctacatgtt attacgtgaa
    20341 aaaatcaaag aaatctcctt ttctccttca gaaaatgaag tgattcagtt tctcttaagc
    20401 gattctccag cacttggcga aatgaccatc caagagattg ctgagcgatg ttatgtccac
    20461 ccgtccacac tcatccgtgt agcaaaaaaa atgggttttg atggctgggt cagttttcgt
    20521 aatgcttttt tagctgaaac agcctatctt gaagggaatt ttcaagacgt agatgcgaat
    20581 cttccttttt ccaccacaga cggtattatg acgatcgcaa ataaaatggc ttctttagaa
    20641 cagatgacga ttcaagatac actttctctc attcatcatg accaactgca aaaagccaaa
    20701 caactaatgc ttcaagcaaa aaaaatcgtg attttcgcca gtaactcgaa tactttgatt
    20761 tctcaagatt tcatgctgaa aatgaagcgc atcaaaatgg atgtcacgat tgtcaccact
    20821 tttggtgaat cgttctatga agcgtacaat tgcacgagtg atatgtgcgg gattctgatt
    20881 tcttatacgg gtgaaaacaa tgtgatcctt cgtaccgcag aaatattgaa acaaaatcat
    20941 gttccaattt tagctttgac cagtatcggt gattcaacgc tatcttcaat gagtacagcc
    21001 gctttgcgac ttacgacacg cgaacgactg tattcgaaaa ttggtaattt tacgatcaat
    21061 acatcgattt gttatcttct agacgtgtta tatagttgtg tttttgcaga aaattatcaa
    21121 cagaatttag tccatctaat ttctgtagga caaaaatttg ataaacgccc tatcagctca
    21181 gccatcatga aagagccttt caattttgaa aataaagaag tggactaaaa agaataatta
    21241 agcctagtcc attctttcac ctaaccgttc aggtcagtgc ggtgataagt gcatcgtctg
    21301 gcccgaccgt ctccccttga ttttccaaaa tatccaaata atccgtgtaa ttggtgacga
    21361 tcacaggtgt ttctaaactg tatccttcct cttcgatttt ctttttgtcg aagcggagca
    21421 acagatcgcc ttgtttcact ctttctcctt gctgcaccaa gctttcaaaa tatttccctt
    21481 ccaactgaac agtatccaat ccgacatgaa tcagcaattc cagacctgct tcagaaacca
    21541 ttccgattgc atgtttcgtc gggaacaggg tcatgactgt tccgtcaaaa ggggcacgta
    21601 cttctccttt ttcagggtag atcaagattc ctcgacctag tgttccttgt gcaaaagctg
    21661 catcctttgc tgcattcagt ggagcgattt gtccttgtat cggtgttttt acaatttctt
    21721 tacctgatct tttctgtcta tcatctgttt cacctgcttc ctccttccaa aagaaaaaag
    21781 tcagcgcaaa ggataccacc atcgcaatgg cgattccaat caatacatta atcacatccg
    21841 ttaaatcacc atctggtcta atatagttgg gaattgtaaa gatgcctaat ccacctggat
    21901 tccaagaagt taaattcatt aatccactat acgcgccccc taacgcacca ccgacacagg
    21961 taaaaataaa tattttttta cgtggcaatg taaacccata aatagctggc tctgtaatcc
    22021 ccacaatacc agaaacaatg gctggtattg cgattgcttt gtcttttggg tttctaagct
    22081 taaagtagag tgcagtaagt gctgcagttt gtccaaaaga cactactgca acaggtgtca
    22141 tgatattttg ccaaccattc acagaaaatt gcatcaatcc cattggtacg attgcccaat
    22201 ggagaccaaa catcactaga atttgccaga agaagcctac gattgctcca taaataactg
    22261 gcgaaagtgc taaaagtgtt tcaaatccag ccattaaaat atcagttgcg aatgtgaatg
    22321 ctggtccaac gagtaaaaaa cctaagggca ttgtaataag aacggtgaag aatggaacga
    22381 agaaattcgc gatcatctca ggtactaact ttttaattgg tttctgaatt tggctagcta
    22441 acaacacaat aaagataatg ggaatgacag aagaagcgta gttacgcgca atccagggaa
    22501 tgccgaatac tgtttgataa ataggtgctt caaaaatagt gccagaaaat atggtagtca
    22561 gtggtgcttc tgctgcacca ctcaaagctt ccagcgacaa gctaggattc attaatgctg
    22621 ccccaagcaa catccctacg aattcattta ctttaaattt acgggcagct gttaaaccga
    22681 taataacagg cataaattgg aatacagaat caccgatatt atcaaacata acaaatgtgc
    22741 cagaaccgcg gtcaaatgcg cctaagaaga caagtaatgc tgtcagtcca cgaatcatcc
    22801 caccagcagc tagaatagca aggaatggct ggaaacagcc cgatattcca tcaataagca
    22861 tatcaaacgg tctcattttt tctgttgaaa cagcctcatc aaaagtaagg tttcctgctt
    22921 ttataacctc ttcaaacacg actggcacat gattcccgat gacaacttgg tactgtcctc
    22981 ccgctcgcat taccgtcaca acaccgtcca tatttttcaa gatctcatca tttgcttttt
    23041 cttcgttttt taatttgaaa cgaagccttg tgatacaatg tgtcaaacca ttgatatttt
    23101 ccttcccgcc gacattttct acaattttgc tcgctaattc ttgatatttt cccatttcta
    23161 ttccctccaa tttattagtg gaaggtttag ccaactgaat gtcactaccc ttatttcatg
    23221 cgttcttgtt aatttatcct ccttttttta tttatttaca acgattctca tgcactaaac
    23281 gcgcaatatg gatcgttaaa tacatttttt cgtccgtgga catctggtag ttgaatatct
    23341 cggctaaaaa gtctgctatt ttctcaacgc aaccaaacgt atccttatat tttttttgaa
    23401 taacctggta caattcctca gaaacttctc ccacttctga tttttctcca tctaggacgc
    23461 gtgtcagaaa aaaacgtagg tgtgtgacga atcgaaaaaa atggactgaa tcttcatcaa
    23521 atgtaatctt atagtaatag cggacgatat ttagaatatc ctgtattgtt gtagtaaaca
    23581 ggtaggtatt gtcttgttgg ctttgaatgc cttgtgcatt gactaaatgc aatgcgataa
    23641 atccggcttc atcttctgat aacttgatgt ggtatttatc cgagattttc ttcactgttt
    23701 ttttcccgat ggcatactct ttaggaaata gttgtttgat atcatacagc aatacatttg
    23761 gtacaaatat tcccattttg tgacgttcga tagccgtata caaatgatcc gtcaatgcaa
    23821 tatacaccga atcattcaac tggacttgta gagcctcttt tgtatattca atgatttcac
    23881 aagaaatttc taagtactca agaggaatcg cttgtgccaa ttcttggaac tgttgtgaaa
    23941 gtccgtcatt tgtcaactga aattttttgt cgatgcatga gtcaaaaatc gggtcaccca
    24001 ctttcttttt gaatgccaaa ccttttccca tcacgatcat ttcctgattt ctttcatccc
    24061 tggttattac gacgttgtta ttcagtactt tctcaatcag catgttttac tcctttccat
    24121 gatatatagg gctgtatcca aaacaaaaag aaaaacccaa aaagccgtcc atggaaccat
    24181 gggagactca tcaggtttag cttgcatcag gcaatcacta tccaagttgt atcgttttct
    24241 ttatattatc gaaagcaagc acttttgtca acgctttctt agcggctatt gggcacttct
    24301 gactagctgt cgtgattttt tttaaatctg gtaattacga cactaacgct cacagatgaa
    24361 gaatctggga agctctgttt tattttcact ctagcgctta ggtcaaaatg ctactatgct
    24421 actgtcccaa cttctaatat acggtgttcc agtaccagct tatcggtgat aagccaaatt
    24481 tatgcaaaaa tctggaaagc cttttttaca atttttttct tatcacctaa agctgaacgg
    24541 agccctgtca caaccacttt ttacacacca ctgctttgca gtttgtacat atctgcatac
    24601 aaaccatttt gcgctagaag tgtttcatga ttcccacgtt ctactatatg acctttgtct
    24661 aaaactaaaa tcaattctgc atcccgaatc gtggacagcc gatgggcaat cgcaatcgtt
    24721 gtccgtcctt gtcgcatttt tgtcaatcct tcttggatca atccttctgt ttcagtatcg
    24781 atatttgctg tcgcttcatc caatactagt attttaggat ctgtcacgat tgttcgcgca
    24841 aaagtgatca gctgtcgttg tccgctagaa tagctggcac cgtgttcgat cacttttgag
    24901 tgatatcctt ttggtaattc atcgatgaac ttgtttgctt gaacaaactc agctgcatgg
    24961 cgaatctgtt cgtctgtgat agttggattc atcatccgga tatttcctgc gatatctcca
    25021 taaaacaaga aggcatcttg caagaccaat cccattttct tacgtaactc ttccattgga
    25081 tattctcgaa tgtcatgatc atcaatcaag atctggcctt cataaaattc gtaaaaccgc
    25141 atcaatacgt tgatgatcga gctttttccg cttccggtgt gccccactaa tgcaacggtc
    25201 tctcccggat tagcgacaaa agagatattg ttcaatacat tgtgctctcc atcataagaa
    25261 aagcttacat tgcgaaactc gatcttacct gaagtaatcg cttgatctgc tccaggattt
    25321 tgttgcggtg catattcttc tgtatccatg attttcaaaa tacgactacc ggcaaccatt
    25381 ccatctgtaa aggtgctcaa aaagtccatc atttgagaca ttggattaaa aaatccctgg
    25441 acataggtcg taaatgcata gatcagtcca acttctacgg gagaatgcag cgcatcgatc
    25501 ccaaacaaag tcaacgccaa cgcaatcgcc aatgcatata gaaaattgat gatcggactt
    25561 aacagaatcg aatttgtttt gatcatcgca tagcgcgtgt ctaaatactc gttgttcact
    25621 tgttcaaatt ctttagctaa acgagcttct tggcgaaact gctggataat acgcatgccg
    25681 gaaatcgatt cattcaattt cgtgttcagc tgacttaatt tttcccgcat attccggtaa
    25741 atacgagaac tgaatttctg gtaataccag atgacgattc ctaaaatcgg caagaacacc
    25801 aaagtccaca aggcaatttc tccattgatt tgaaacatgg caatgaagga agaaacgact
    25861 gcaaagatcc cagtcaatac catcaaaaag acataccaaa attcaaatag cgtttctgtg
    25921 tcatttgtca ctcgagaaac agtcgatcct gccggtgtct gatcaaaata acgcatacct
    25981 agtgtatgca gcttttcaaa taatcttacc cgaacatact gataggtctt caatgaagcc
    26041 atggaataca aataccattg gaaaaaccaa atgatacttt tgactatgac tccaaacaag
    26101 tacataccag caaaaaacaa aataacttgt gtcgtagccg actgtttagc cagatgatca
    26161 tccataaaag tttggatgat ccttggcaat aggacattga tcacagacag ggcaaacgca
    26221 aataagatag caacgatgaa cgttcttcta aaaggtttgg cgaacttcat cagtcgtttg
    26281 atgatcttct tctgttcttt aagtggaatc gattttgtcc attccgattg ataattttca
    26341 tccatcaggc ctcgcctcct tctatcttcg cttctaattt ctgtttctcc cacattcgtt
    26401 cataccagcc gcctaatgca agcagctcat gatgggttcc tcgttcaatg attttccctt
    26461 gatcgacaac aatgatttct tttgcatgca tcacgctgct taaacggtga gctgcaatga
    26521 tggttgtttt ctcttttctt atttctttta aattatgcaa gatagcttct tctgttttgg
    26581 catctactgc agaaagtgca tcatctaaga tcagcaattc aggatttttg atcaatgctc
    26641 gagcaatcga aatcctttgt ttctgtccgc cagataaaga gactccacgt tctccaacta
    26701 aggtatcata accttttggt aattccttga tttcattgtt gataaatgct aaagaagccg
    26761 catgctctac ttcttcttct gaaagttttg gattagaaaa acggatatta tctcgaatgg
    26821 tcatcgaaaa caagaagtga tcttgaggca catagccgat tgatcctaat aaagcattca
    26881 atgaatattc tttgatcgaa catccgccaa attgaatttt tccttgataa tcatcgtatt
    26941 cacgcatcag taatttgatg attgtcgttt ttcccgcacc agttttccca acaataccta
    27001 atgtttctcc ttcttggatc gcaaatttga tttgttctaa tgctggtgat tcttctttcg
    27061 gatagttaaa agaggaaata gctacattca gctcgccttt tgccggagtc tgcactgcac
    27121 ccagttgttc cacgatatgc gtcttttcat gcaaaagttc attgacgcga tcataagaag
    27181 catttcctct ttccaatacg ttgaacaatc gaccaatcgc aaacatcggc caaacaagca
    27241 taccgatata gctaataaat gagaccatct gcccgattga gatcgtgcct tccaagacat
    27301 actttccacc taaaataatt gttgcaacat aagacagacc gataatcaag gtaatgaatg
    27361 gatcaaataa ggagtctaaa aaattgactc gtttgttttt aataattgca tcttcaatct
    27421 tttcttcaaa atccgcgaga tcttcttttt cttgtccaaa tgttttaatg actttgatcc
    27481 cagtaatact ttcttgtgtc ttatcattga tactagaaaa agcttcttgc gaatcacgaa
    27541 aagcatcgtg gagttttgac cccagcactc gtgaagtaac ggctagtaaa ggcagaggaa
    27601 tcagcgcaat cagtgttaag cgccaatcaa taaacaaaat catcgcaatg atcgttgttc
    27661 cgcccgtgat cactgaatct gcaaaggtca agataccagc tcctgctaca ttttggattg
    27721 cattcaaatc attcgttgca tgagccatca aatctccagt ccgatatttt tggtaaaaga
    27781 gattgtccat ttctgtaaaa tgtttgaata gctgctgccg aagttctttc tccaaccgag
    27841 ctgcacttcc ccaaatattc gtccgccaga tataacgaaa aagatattgt aagagagctg
    27901 caaggatcaa taataagatc caccaggaaa tgatctttat tgataaattt ttatcggcga
    27961 tctcatcaat gatgatccca atcacttttg gaggagctaa ctgtaccaag gccactgcga
    28021 atagggagaa aatcccaatt aaataatgtt tcttttcctg tcgaaaaaac caacctaatt
    28081 ttctaaaaat cgacatttta ctcctccttt gtctttatca agagcatagg attgtctgtg
    28141 cttgtctttt ttgctgaata gtctgaatag tctaacaaat tacgaaataa aaaacggact
    28201 gaaaaaatca gcccgcaaaa agtctttggg tcagaaccta ttttttccct tggttcttca
    28261 tttgctgcat catctggcga attttcttct cagatggttt ttgccccatt gacatcatca
    28321 ttgatcgcaa catgtcctca ttaacgggag gatttttttc taaatagtct ttcatatatt
    28381 tacgcgctag gaaaaagcca cctactgcgc cgattagcaa agcaataata gcaataagta
    28441 cgacaatacc tgttgatacc atataaatct ctcctttcta tttccgaatt gctctcaatg
    28501 atttatttta ctaaagttta ttgaaaaaag aaaggcgtat tctttagttt catgcaaaaa
    28561 atagtggttt ttttagagaa gaacaaaatt tctttcatta accagcaaga cacgccacga
    28621 aaaaaaatta ggcataccaa atctttttct ttaatttttt ctttctgagg tgtagaatgt
    28681 aatcgtagat aaataggaaa ggtggttttt tcttgaataa tcaatccaac aaacaatttc
    28741 ataagcatcg acttattgaa catacaaatg gtttatcttt agctgaagta aatggaacag
    28801 tggatgtacc caagggaaaa agttttttta aaacacttct tgcttactct ggaccaggtg
    28861 cattagtagc agttggttat atggatccag gtaactggtc cacttcgatc actggtggac
    28921 aaaatttcca atatttattg atgtcagtta ttttgatgtc tagtctgatt gctatgcttt
    28981 tacaatatat ggcagcaaaa ttaggaatcg tgacccaaat ggatttggcc caagcaatcc
    29041 gagcacggac aagtaagaca ttgggtgtca ttttatggat cttgactgaa ttagccatta
    29101 tggcaacaga catcgcagaa gtcattggtg cagcaatcgc cctctacctg ctttttgata
    29161 ttccattggt aattgccgta tttatcactg tgttagatgt tttcgtcctt ttattgctga
    29221 caaaagtcgg gtttcgtaaa atcgaagctt tggttgtctg cttgattttc gtcattttat
    29281 tcgttttcgt ctatcaagtt ggcttgtcta accctgattg gcacagtttg tttgccggat
    29341 ttatccctaa cagtcacaca ttctcagact caccggtcat cggcggcgaa actccgctga
    29401 ctggagcttt agggattatt ggtgcaacag tcatgccgca taacttatat cttcattcag
    29461 cagtttctca aactcggaag atcgatcacc aagacgaaga agatgtagca caagcggtac
    29521 gcttttctac ttgggattct aatatccagt tgtcaatggc ttttctagtc aatgcactgc
    29581 tattgatcat gggtgtggct gtattcaaaa ccggttctgt ccaagatcct tccttttttg
    29641 gtctgtatga agctttatct gatccatcag ctatgagcaa tggtatcttg attgctgtag
    29701 caaaatctgg tatcttgtct actttatttg ctgtagcact tcttgcttct ggccagaact
    29761 ctactattac tggtacgttg accggtcaag tcatcatgga aggcttcatc catatgcgta
    29821 tcccaatttg gctaagaaga ctagtcacac gattgctttc tgtgattcct gtattgattt
    29881 gtgttttgtt tacaagtaca aaaggaacga tcgaagaaca taccgctttg aataacttga
    29941 tgaatgaatc ccaagttttt ctggcttttg cattgccatt ctcgatgatt cctttactga
    30001 tgatgaccaa tagccaagcg gagatgggga gccgattcaa aaattcattt gttgtaaaac
    30061 tacttggctg gttctctgtg attagtctga cttacttgaa tttacgagga ttaccaggtc
    30121 aaatcgaaag tttcttcggc gagcaagcca cctcagctca actagcattg gctgatcaaa
    30181 tcgcttatgt aatcattgtt cttgtccttc tcctgttggc atggacgatt ggtgatttat
    30241 ataaaggaaa caaaaaatat gaacagcgtt tgcaggaaac gatagaagaa atcgagtaga
    30301 aactgattct tttacacaaa ggaggttgtg tcagaagtgg gcagcttcaa gaaataagaa
    30361 ggaatttaag aaaattgctt ttcaaatttt tgtgaaattt cggcttattt ccgaagaagt
    30421 tacttctgga acaccgtgta tcagaggttg tgaaaaagct gccttgcatc aagtaataag
    30481 aacagccaag taaaaacagc tttttatgtt tttaacttgg ttggacttat taccgcagat
    30541 gcaagctttt gagcaccgtt tattcggaaa aaggaactgc gacaagactt ttgtcacagt
    30601 tccctttcct acgattagtc aagtaaaagg tgtaagaaaa atagctcata aacgaaagcg
    30661 acaaaatcaa
//
LOCUS       DAYYKN010000016        30460 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000016 DAYYKN010000000
VERSION     DAYYKN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.016"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..767)
                     /gene="pyrE"
                     /locus_tag="ACHBB7_000625"
     CDS             complement(138..767)
                     /gene="pyrE"
                     /locus_tag="ACHBB7_000625"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM9208600.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(774..1490)
                     /gene="pyrF"
                     /locus_tag="ACHBB7_000626"
     CDS             complement(774..1490)
                     /gene="pyrF"
                     /locus_tag="ACHBB7_000626"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM9208601.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1487..2419)
                     /locus_tag="ACHBB7_000627"
     CDS             complement(1487..2419)
                     /locus_tag="ACHBB7_000627"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM9208602.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2416..3192)
                     /locus_tag="ACHBB7_000628"
     CDS             complement(2416..3192)
                     /locus_tag="ACHBB7_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM9208603.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3246..6431)
                     /gene="carB"
                     /locus_tag="ACHBB7_000629"
     CDS             complement(3246..6431)
                     /gene="carB"
                     /locus_tag="ACHBB7_000629"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM9208604.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6436..7512)
                     /locus_tag="ACHBB7_000630"
     CDS             complement(6436..7512)
                     /locus_tag="ACHBB7_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM9208605.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7517..8797)
                     /locus_tag="ACHBB7_000631"
     CDS             complement(7517..8797)
                     /locus_tag="ACHBB7_000631"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM9208606.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8816..9742)
                     /locus_tag="ACHBB7_000632"
     CDS             complement(8816..9742)
                     /locus_tag="ACHBB7_000632"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM9208607.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9755..11038)
                     /locus_tag="ACHBB7_000633"
     CDS             complement(9755..11038)
                     /locus_tag="ACHBB7_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM9208608.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11052..11591)
                     /gene="pyrR"
                     /locus_tag="ACHBB7_000634"
     CDS             complement(11052..11591)
                     /gene="pyrR"
                     /locus_tag="ACHBB7_000634"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM9208609.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11826..12737)
                     /locus_tag="ACHBB7_000635"
     CDS             complement(11826..12737)
                     /locus_tag="ACHBB7_000635"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9208610.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12734..13198)
                     /gene="lspA"
                     /locus_tag="ACHBB7_000636"
     CDS             complement(12734..13198)
                     /gene="lspA"
                     /locus_tag="ACHBB7_000636"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM9208611.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13203..13901)
                     /locus_tag="ACHBB7_000637"
     CDS             complement(13203..13901)
                     /locus_tag="ACHBB7_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM9208612.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13888..14274)
                     /locus_tag="ACHBB7_000638"
     CDS             complement(13888..14274)
                     /locus_tag="ACHBB7_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208613.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14421..16091)
                     /locus_tag="ACHBB7_000639"
     CDS             complement(14421..16091)
                     /locus_tag="ACHBB7_000639"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM9208614.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16274..16507
                     /locus_tag="ACHBB7_000640"
     CDS             16274..16507
                     /locus_tag="ACHBB7_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9208615.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16606..17037)
                     /locus_tag="ACHBB7_000641"
     CDS             complement(16606..17037)
                     /locus_tag="ACHBB7_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM9208616.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17116..17529
                     /locus_tag="ACHBB7_000642"
     CDS             17116..17529
                     /locus_tag="ACHBB7_000642"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM9208617.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17567..18715)
                     /locus_tag="ACHBB7_000643"
     CDS             complement(17567..18715)
                     /locus_tag="ACHBB7_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9208618.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18837..19433)
                     /locus_tag="ACHBB7_000644"
     CDS             complement(18837..19433)
                     /locus_tag="ACHBB7_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9208619.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19633..22275)
                     /gene="alaS"
                     /locus_tag="ACHBB7_000645"
     CDS             complement(19633..22275)
                     /gene="alaS"
                     /locus_tag="ACHBB7_000645"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM9208620.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22663..23094
                     /locus_tag="ACHBB7_000646"
     CDS             22663..23094
                     /locus_tag="ACHBB7_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9208621.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23139..24488)
                     /locus_tag="ACHBB7_000647"
     CDS             complement(23139..24488)
                     /locus_tag="ACHBB7_000647"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9208622.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24558..25721)
                     /locus_tag="ACHBB7_000648"
     CDS             complement(24558..25721)
                     /locus_tag="ACHBB7_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9208623.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25850..26173
                     /locus_tag="ACHBB7_000649"
     CDS             25850..26173
                     /locus_tag="ACHBB7_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208624.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26356..27705)
                     /locus_tag="ACHBB7_000650"
     CDS             complement(26356..27705)
                     /locus_tag="ACHBB7_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9208625.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27774..28727)
                     /locus_tag="ACHBB7_000651"
     CDS             complement(27774..28727)
                     /locus_tag="ACHBB7_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM9208626.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28829..30019)
                     /locus_tag="ACHBB7_000652"
     CDS             complement(28829..30019)
                     /locus_tag="ACHBB7_000652"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM9208627.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(30219..>30460)
                     /locus_tag="ACHBB7_000653"
     CDS             complement(30219..>30460)
                     /locus_tag="ACHBB7_000653"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208628.1"
                     /translation="KNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVFL
                     LVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         8553 a         6686 c         5347 g         9874 t
ORIGIN      
        1 caaaaactga tcacgataga agtttctttt catacctgat gaaaagatgc ttctcgcaat
       61 cagtttttgg atttttgcta ttccaattga tgattctgga gacacaaatc ggatttctct
      121 atcttcatca agcgcgctta aatctgccaa ttttcaggat cttttttcca ttcttttagc
      181 agagttaatt cttgctcatc gatataacga tcttctaaag ctgcttcaat caatacagaa
      241 taattggtca acgtcataag agggatttcg gctttttcga aatttgcttt tccttttggc
      301 aactcatacg taaagatagc cgcaacacca aggatatccg caccttctct ttttgctgct
      361 tcagctgctt ccaagacact tccgcctgta gaaatcaaat cctcgatcac aaccatcttc
      421 tgaccttcaa atatacgtcc ttcgatttgg ttacctttcc catgatcttt cgccttactt
      481 cgaatataga ccattggcaa atccagtatt tccgctaccc atgcggcatg agggattcct
      541 gctgttgcag ttccagcaat cacttgtact tcagggtaag cttctttgat tttgcttgct
      601 aatccttttg cgatctcttt tcggactttt ggatagctca tcgtgattcg gttatcacag
      661 taaattgggc ttttgattcc acttgcccat gtaaaaggat tacttggatt taaaaatact
      721 gcttcaattt ctaataagtc ttttgcgata ctttgttcaa cattcatttt aggttagctc
      781 cattctgttt gaatcgtttg atatgcttta taaggattag ttgactgagt gattggtcga
      841 cctataacga taaaagttga gccgatcttt tttgcctgag aaggtgtgac tactcttgtc
      901 tgatctcctg cgactgttcc ttctggtctt atacccggag ttaagcaaac aaattcgtct
      961 cctgccattt ctttgatcgc ttctacttcc caagcagaag aaaccacacc atctaatcct
     1021 gcttttttgg cacagctagc ataatggatc acactttttt ctaaagaaac ttcgattagt
     1081 tgatcggcat gcatttcttc ttcacttgta gaagtaagct gagtgatagc tagaagtagc
     1141 ggacgttttc caccttctgg agtcccctct tctaatcctc gcattgccgc ctccatcata
     1201 cgaataccgc cagcggcatg aacacaggtg agatcaacac caagctttgc caatccccgc
     1261 attgcttttt cgaccgtatt cggaatatcg tgaagtttca gatctaaaaa tacattatga
     1321 ccagcctctt ttagataacg tacgatttca ggtccttctt ggtagaacaa ttccattccg
     1381 attttcacaa acaatttttc ttctttcggg aaaaatcgaa gaaagtcttc tacctcttgc
     1441 ctagttgaaa aatctaatgc aataattggt cgttggtcta gttgattcat cgttcacgtt
     1501 cctctctcac ttctttgatt aatctttcca gcgattcgat gcctagttcg ctcattcgca
     1561 cagggagctc tttaattagt ttaggacaga tatacggatc ggtgaaattc gcggtcccta
     1621 tagctacagc acttgctcca gccataaaca tctcaagaac atcatccact gtctgcacgc
     1681 cgcccatccc aatgattgga aggctggata tacttgctac ttggtgaatc aaacgaatag
     1741 cgacaggctt gattgctggt ccggagagtc cacctgtttg attggctaaa atcggttttc
     1801 ttgtcttaag gtcaatccgc ataccaagaa gagtattgat catcgtaaac ccatctgccc
     1861 ctccagcttc gattgcctga gcgattggaa cgatgtctgt tacatttggg gacaatttga
     1921 catagacagg aacagaagaa actttcttta ctgcttgtgt taattgaaaa gcaacatcag
     1981 aatctgttcc aaaagcaatt ccgccatgtt tgacatttgg gcaagaaatg ttcagctcga
     2041 ttgctttcac atttggtgct tcgccaattt tgctacaaac ttctacatag tcttcttcac
     2101 aagcaccagc aacatttgcg atgatgggta actcattgta ctcctctaat tctggaagaa
     2161 gtttatgcat aacaacgtct agaccaggat tttgcaaacc aattgcattc aacatgccag
     2221 atggtgtttc cgccactctt ggcgtgggat tgccaaagcg ggcatttgga gtcgttgcct
     2281 tgaccatgat cgatccaagc tgattcaaat catagtattt tccgtattct ttgccaaaac
     2341 cgaaacagcc gcttgccggc atgataggat tttttaattc taggccagga agtttgaccg
     2401 ctaaaggatt ctcattcata taattacctc tcctatttta aaaatcggtc cttcgtcaca
     2461 aactttcaca ctttttgttc ctgtttcatc ttccggcgta tgacaaacgc aagcatagca
     2521 agcccctatc ccacatgcca tgcgggattc taatgataac tggacatttt gatgcgaaga
     2581 aaacagctgt tccactgtct tcaataaccc gtttgaacca catgcaaaaa cagctgtagg
     2641 ttccgtcttc gttcctaaca ataaattacc gacatttccg tgcataccgt atgttccatc
     2701 gtcagtagcg attcttgttt ctcctagacg attaaattct ttttcataat aaacgacttc
     2761 atgtgtggca aaacctaaaa aatgcgttgg cttaattcct ttttcgacta actgttttga
     2821 taactcatat agaggaggga caccgattcc tccaccgatt ataaatactt cgtctcctgt
     2881 atgaagattg gacaaatcaa aaccattgcc taaaggaccc atgacatcta aatggtctcc
     2941 ggcagaaaga gtagagaaga ctctagtccc ctcaccttcc acccgataaa tgatacggca
     3001 agtatttgtt tcgtggtcga tatgattcaa gctaatcggt cggcgtaata gcaagtctgc
     3061 acgcggcacg agaatatgca agaactgtcc tggttgctgc atttctttta ctagatcacc
     3121 tttcaatgtc atttcaaaaa tctttggtgc cagctgtttt tgctgaacga tttccatcat
     3181 ttcttgtttc atctgtcccc tcctgcaacg gtttttcgtc tttttgtctt aacatgtgct
     3241 cgccgttaaa tagggttagt tgaaaaagca cgggattcca tcactcgaac gatcgcatct
     3301 gctgtgtcta aagaagtaaa caatggaata ccatgttcaa ctgcttctct tcggatgatg
     3361 aaaccgtctt tagaagcatt tccccgatct ttatcaatcg tattgacaac cacttgtgtt
     3421 ttaccatctc ttattaagtc gactacattt ttttctgctg cttcctcaga aattttcgct
     3481 actgtttcaa cgaccagacc atttttttcg aaataagcag ctgtatgttt cgttcctatc
     3541 agactatagc caatttctgt aaaacgttta gctaattcta gtgcttcttc ttttgtttcg
     3601 tccgcaatcg taaataatac ggctccatat tcaggcaagt gtaatccaga agcttcgaat
     3661 gccttgtaca aagctttgtc taattgtctg tcagatccca ttacttctcc agtcgatttc
     3721 atttcaggcc ctaagtaagt atcaactttc tgcaatttcg taaaagaaaa gacaggtgct
     3781 ttaacatgaa cttcttttgt ttctggataa agtccgtttt cataaccaag tgtctttagt
     3841 ttctctccca aaatagcttt cgtcgcaatt tgtgccattg gaattcctgt caccttactt
     3901 aagaatggaa cagtccgact tgctcgagga ttgacttcga tcacataaac ctcattttca
     3961 tggatgacaa actggatatt catcatcccg acacaattca aacctaatgc taagcggatc
     4021 gtgtaatctt cgatcgtttt cttgatcgat tcagataatg tttgaggtgg atacacagcc
     4081 attgaatccc ctgaatggac accagcacgc tcaatatgct ccatgatccc tgggatgagt
     4141 actgtttcac cgtcacaaat cgcatccact tcgcattcac caccaagcaa gtaacggtcc
     4201 actagtactg gatgttctgg agatgctttc acagcatgtt ccatgtaatc ttctaaatct
     4261 cgctggtttt caacgatttc catcgcacgt ccgcctaata catagcttgg acgaacaaga
     4321 actggataac caattctcga agcgataacc acggcttctt ctttacttgt agcagtatcc
     4381 cctaaaggtt gaggaatatc caactctttc agcgcttgct cgaataaatc acgattttcc
     4441 gcacgatcca agtcttcgat cgttgtccct aagattttta caccagcagc agtcaaaggt
     4501 tcggctaagt tgattgctgt ctgaccacca aattgaacga ttactcctat cggctgttct
     4561 agctcgatca cattcatcac atcttcgaag gtcaatggct caaaatataa tttatccgaa
     4621 atagaaaagt cagtcgaaac tgtttcagga ttgctgttca tgatgattgc ttcgtatcct
     4681 gcctgctgga ttgctttgac agaatgcaca gtcgcatagt caaattcaac cccttgtccg
     4741 atacggatag gaccagatcc cagtactaga attgagggct tttctgatcg tgaactttca
     4801 ttttcatatt cataggaact ataaaaataa ggtgtttttg attcgaattc cgctgcacaa
     4861 gtgtcaacca tcttatatac aggaatcaaa tcattttctt ttctcatctg gcgaacggct
     4921 tcttcgttta tttcccacag ctccgccact tttttgtctg taaaaccgtt ttgtttcgct
     4981 gttttcaata tcttgtcatc caacggctgc tgtttcaact gctgttcgat ttctacgata
     5041 tgcaacagtt tatctaaaaa gaataaatcg atttttgtca aatccgctaa ttcttctatg
     5101 gtgtaccctc ttctaatagc ttcagctaca taaaacaggc gatcatcttg tgctcgaacc
     5161 actttttgga ttaggtcatc gtccgtgatg tctgccaact caggaagttc gatatggtgt
     5221 gtaccgattt ctaaagaacg cacagctttc aataaagact cttcgatatt ccgaccgata
     5281 gccatcactt caccagttgc tttcatctgc gtgcctaaaa gccgttctcc gttttcaaac
     5341 ttatcaaacg gccagcgagg aattttagct acaacataat ctaatgcagg ttcaaattct
     5401 gcaaaagtcg ttcctgtaac aggatttttc atttcgtcta atgttaatcc aagcgctatt
     5461 ttagcagcta gcttagcgat cggatagcca gtcgccttac ttgctaacgc agatgaacga
     5521 gaaacacgcg gattgacttc aatcacataa taattaaaac tatgtggatc aagagcgagt
     5581 tggacattac agcctccctc gatcttaagt gctcgaataa tcgaaagaga agcatcacga
     5641 agcatttgat attcatggtc agacaatgtt tgactagggg caaatacgat agaatcacct
     5701 gtatggatac ccaccgggtc aaaattttcc atattgcaga caacgattgc attatcagcc
     5761 gaatctctca tcacttcata ttcaatttct ttaaaacctg caatacttcg ttcaatcaaa
     5821 cattgggtag cgggggaaag acttaatcca ttttctgata cgatacgtaa ttcttcttca
     5881 ttttcacaca tacctccacc agttccgcct aaagtaaagg ctggacgaac gatgacagga
     5941 tagccgattt tatttgcaaa atctacagct tcttctactg tattgacgat ttcagactcg
     6001 gggattggct gattcaattc tttcatcaac tgtttgaata gatcacgatc ttctgcttga
     6061 tcgatcgcag aaagctttgt tcctagaagt tctatgttca attcttctaa aatacctgat
     6121 gctgccagct ccattgccat attgaggcct gtctgtccgc ctaaagttgg aaggagagca
     6181 tctggacgct cttttcgaag aatccttgat acaaattcaa gtgtgatcgg ttcgatatat
     6241 acttgatctg ccacttcttt atccgtcatg atcgtcgcag gattagaatt tactaaaacc
     6301 acttcatatc cttcttcttt taaggcaagg caggcttgtg ttcctgcgta gtcaaattct
     6361 gcggcttgac caatgatgat cggaccagat ccaatcacca taattttctt gatatctgta
     6421 cgtttcggca tctgtttact gctcctttcc tgcgtccatc atttccataa attcatcaaa
     6481 taaatgtagt gcatcatgcg gaccaggggc agcatctgga tgaaactgca cactgaaagc
     6541 gggataatct ctgtgtttga ctccttctac tgttccgtca tttacttcta catgagttac
     6601 gatgagtttt tctggatcaa tcgtggcttc atcgactgca tagccatgat tttgagaagt
     6661 aaaatcaatt cttccggtcg ctacttctcg taccgggtga tttaatcctc gatgtccgaa
     6721 tttcatttta taggtatctg caccgttggc taaagcaaat aactggtgac cgagacaaat
     6781 accaaaaatc ggtacttttc cttggatttt tttgatcatt tcgattgctt ctggcacatc
     6841 ttttggatca ccaggtccat tcgtcagcat gactccgtct ggtgatagcg ctaaaatcgt
     6901 ctcggcatct gtgttatagg gaagtactgt gaggttacag tttcttttac tcaattctcg
     6961 taaaatacta tgtttcaagc cgaagtcaat gacaatgact ttccgacctg ttcctggact
     7021 tggataaggt ttgttagttg agacttgctg cacttgattg gttgctagaa cggttgcttt
     7081 cagctgatca aatgcatgtt cgaaggaatc tccagcatca actatactag ctttcatcgt
     7141 tccgacttca cggattttgc gagtaagggc gcgcgtatcg atacctgata ttccaggaat
     7201 tcctttttgt ttcaaaaatg aatctagcgt catttggttt cgccagtttg aagctacacg
     7261 tgcatgttct tttactacta ctcctttgca tgttggagaa atcgattcat aatcatctct
     7321 atttacccca taattaccca ccataggata ggtaaatgta atgatttgac cgttaaaact
     7381 ttgatcagtg atagtttctt gataaccagt catacttgtt gtaaaaacga tttctccgaa
     7441 gacactagca tctgcaccaa aagcctttcc ttcgaaaact gttccatctt ctaatataag
     7501 caaacgttcc atacttttat tctcccttct gccacacaag ttttccgttg acaaaagtca
     7561 tttgtgtttc gccttttact ttccagccga taaaaggtgt attctcccct tttgataaaa
     7621 agtcttcttt atcgatcgtg caagtttgtg taatatcaaa aactgctaca tcagcggggg
     7681 caccaaccgt tagcgttcct gcattcaaac caaaaatctc agccggttta actgccaacc
     7741 aattgatcac ttgttccaac gtgaatcgac cagtttctac aaaatgagta tagatcagtt
     7801 gaaacgctgt ttcgcttcct acgataccaa atggtgattt catgaaactt tggctttttt
     7861 cttctaatcc atggggggca tggtctgtag cgatgcaatc gatcgtaccg tcaagcaagc
     7921 cttcgatcaa agcttctctg tcctcccgtc ctcttagcgg cgggttcatt ttccaaaagc
     7981 cgaaatcttc gggaatatct tcgtcgatca aaataagatg atggggtgaa acttctgctg
     8041 ttacgtggat tcctgctttt tttgcgtctc tgattacacg gacactttct ttagtagaaa
     8101 catggcaaac atggtaatga acaccagttg cttcagctag caacagatct ctagcaattt
     8161 gagaagactc ggttacgctt aagatcccgg gaagaccaag ttcttctgcc ttcttgccag
     8221 cgtgcataac gccgccaaat aataaagatt catcttctgt atgggctact agggctttat
     8281 tgttttttgc agcttctttc atagccagat acatcgtccc agctgtttgg acacctacac
     8341 cgtcatttgt gaaagcaaaa gcaccttctt caatcagtgc ttcttgatca actaatttct
     8401 cacttcgcag attttctgtg attggcgcat actgcaatac cttcacaact gcatcttttc
     8461 taatgatgtc atatacttgt ctcagctttt ctgccgtatc aggtacagga tttaaattcg
     8521 gcatcgcgca tacagtagtg aaaccgccac gagcagctga gcgagtccca gcttcgatcg
     8581 tctccttgta tgtaaatcct ggctctctca aatgaacatg gacgtccact aaaccaggtg
     8641 tgatcaattg tccctttgca tcaaacactt catcaaattc ggcttctgag aatcctgtac
     8701 cgattgcttt gattcgccca tcttctatcc aaatttctgc tggtgtcgtc atattttttc
     8761 gtgtattgat ctgaccgttt ttaatgagtg ttttcatttt ttcatcctcc tataattacg
     8821 cttttccatt tagcactgct tctaagattg ccattctgac gaatactcca ttgctcattt
     8881 gctgaacgat ccgcgactgt aatccttcca ctaatgaatc agctaattct acatcacggt
     8941 taactggtgc tgggtgcata atgattgcat gtttttgcaa cttcttcgca cgctcaactg
     9001 ttaacccatg ttcctgatgg tagccttctt ttgagaaact ttcttttcca tcatggcgtt
     9061 cgtgttgcac ccgaagcatc atcatgacat caacttgatc tagtagttca tccaatggca
     9121 tataatgtcc gtacacttcg aattcatcgt cgtaccattc tctaggacct gagaagaata
     9181 cttgtgcgcc tagacgtttt aacatctgca tattggattt agctacgcga gagtgagtga
     9241 tatcacctac gatggcaact ttcagacctt caaaatgacc aaattcttca taaatggtca
     9301 tcaaatcaag caagcattgg gttggatgct gtccgcttcc gtctcctcca ttgataatcg
     9361 aacactggat tgttttactt tgtatcagtt cgtcataata gttctcatca ccatgacgga
     9421 taaccgctac gtctacgcct aatgctgaca tcgtcagtac tgtatcgtat agtgtttccc
     9481 ctttttggac agagcttgtg cttgcttcaa attcaatcac ttctagccct agttttttct
     9541 ccgctacatc gaagctttta tgtgtgcgag tactattttc gaaaaagagg tttgtagcaa
     9601 agtactggcg cttttgcgga gtccaattag caccttgctt gaattcctgc cctctgcgga
     9661 tcaagcccat aacttcttga tcagtaagtg cctcaacggt caataaatgc tttaagctga
     9721 tacgttctga tttgatgatc ataatgtttc ctccttagtt ttcttcgctt cgtgaagttt
     9781 ctggtaagat aagatttaaa atgatgccaa gtacagttgc taatgccatt gccgacaatg
     9841 taaatgtgcc tacttcaaag acgagtccgc caattcctac tactaagata actgaagcaa
     9901 tcaacagatt tttcttttta tcaaaatcga ttttgttctc gattaaaatc ttcattccac
     9961 ttgcggcaat tactccaaat aagataaaac tgattcctga gatgactggt ccagggatac
    10021 ttaaaatcaa agcgcttaat tttcctacga atcctaaaat gactgcaaat actgcagctc
    10081 caccgatcac aaacacgcta tggacacgtg tgatagccag aacaccaatg ttttcaccgt
    10141 aacttgttac aggaggacct ccgacaaagg ctgccacgat ttgtgctaat ccatcacctg
    10201 tcaacgtctt atgaagaccc ggttcttcaa agaaattacg tttagttagt ttattcaata
    10261 ccataagatg gccgatatgc tctgtcatcg tcacaaaagc gatgggtgcc attgttgtga
    10321 ttgctccaag atatagcttt ggttcatatt gtacaaaggg aatctcaaag ttaggaacag
    10381 caaaccaggc agcgtctacg attggcttag tatccacgat tccaaacagc aaggctacga
    10441 tgtatccgct aataatacct agtaaaatcg gaatcaatcc gagaaaacct cttagccaca
    10501 tgttgaagaa aattgttaat cctaatgtga tcagcgctac agcaatatac ttgaaatcat
    10561 acgtgccctg attatacata gcatttgttg ctgcatttgc cgcaagtccc aacccgataa
    10621 ccatgatgac tggaccgacg acgattggag gcaagatttt atccagccat gctgaaccga
    10681 tctttttgat gatcagcgca acaatcaaat agactaatcc agttgtgact gctccttgtg
    10741 caatcgctgg ataaccatct gttttcatca aaagctgcat cgctgcaatg aaagcgaagc
    10801 tgcttcctaa gtacgcaggt atctttcctt tagtagtaaa taagtacacc aatgtaccta
    10861 gtcctgaact gactagcgca atacctggat cgatacctac taagatgggg actaacacgg
    10921 ttgctccgaa catcgtgaac aagtgttgta aacttaatcc tacccaatgt cctggggttg
    10981 ggcgatcgtg gatatctaac accacgtcat cattatgaaa tccttttttc tccgacatgt
    11041 ttctctctcc tctaatcttc ctctttcaaa atcatgatgc gatcttgtcc atcaagctct
    11101 tgcatctcta ctaaaatctc ttccgcccga gatgttggaa tgttcttccc aacataatct
    11161 gcacggattg gcaattctcg atgcccacga tcgaccagga ctgctaatga aatttttctt
    11221 ggtctgccaa aatccattac tgcgtccatt gctgctcgaa tcgttcgtcc tgtatacaaa
    11281 acatcatcaa tcaaaataac ttcttttcct tctaaggaaa ctgggatgtc agaagaatga
    11341 agttccggtt cttctggatt ttcttttttg tcatctcggt ataaagtgat atccagttct
    11401 cctacaggaa tatcgatatc ttctagctgc ttcaggcgtt cggcaattct tgatgcaatg
    11461 tagattcctc gtgttttgat acctaccaat acgatatctt ggatactatg gtttctctcg
    11521 ataatctcat aagtaattcg agtcaatgct cgcttcatgg ttacctgatc tactacttct
    11581 tttgcttgca ttttcattcc ccctactttt ttgcataaaa aaactcctgc cctaagagga
    11641 caggaggaat gtttagactg agcgtatact gatacactga tagctatact atgtcaatga
    11701 gcaatctact tatggcgcac catacgtatc cttgctccca cccttcttag cctcacggga
    11761 ctaaacttaa aggaattcta ttaagtttta tgctttcgtt gttaattact ataccgttat
    11821 ctaaattaaa atgcaatgtt ttttcttaat ttttctaaag ttttttcaaa aacttctgga
    11881 agaggtgctt caaagatcat cttttcgttt gtagtcgggt gcgtaaaacc taacatttgc
    11941 gcatggagaa actgaccatt gcctttcaaa gttttcttcg gtccgtatac tggatctcca
    12001 gctaacggat aaccgatata cttcatatgc acacggatct ggtgtgttct tccggtttct
    12061 agttttaatt caaccagagt aaatccttcg aaacgttcca atacagtaaa atgtgtgaca
    12121 gccgacttcc catcttctct tatcgcctgc attttccggt tttccttcga acggccgatt
    12181 ggcgcattga tggttccttt ttcgtgagga atctctccat ggactaaagc aacatatttg
    12241 cgcaaagagg tcttatcttt taattgctga gctaatgatt cgtgtgcctg gtcatttttg
    12301 gcaaccatta gcagtccgga agtatctttg tcgattcggt gaacaatacc tggacgaata
    12361 acgccgttga tcgtagaaag atccttgata tggtacatca gcgcattgac cattgtgcca
    12421 tgcgtatgac ctgctgaagg atgaacgacc attccttgag gtttgtttat caccgctaca
    12481 tcttcatctt cgtaaacgat cgtcaacggg atattttctg ggaccacatc taatgtttct
    12541 ggttcgggga tcaagacagt aatctcgtct ccttgtttta ctttgtaatt tgctttgact
    12601 ggctgtccat tgacagaaac cgctccattt ttcagccaca gctgaatttg cgaacgactg
    12661 taatcggtga ataaattagt caatacttta tcgatcctac cagattcatg gtgaatcgtc
    12721 gttttaagtt ctgtcatttt tttctttgat ccttttcatc taaaataata taaattaaca
    12781 tacaaatcac tccacataca agtgtcacat ctgctacgtt aaaaatcgga aagctcataa
    12841 aatctgtctg gaacatatct actacatagc caagacgaat acggtcgata aaattgccaa
    12901 tcgcaccagc tagaatcaaa ctcaatccaa tcatgaacca gcgattacct ttttcacgat
    12961 ttttaatcat caatgtgaga atcacaacga ctgcaatgag tgtcactata gtaaagaacc
    13021 acatcttccc ttctagcata ctccatgcgg ctccagtgtt tcgaatatga ttcagtgaca
    13081 aaatacctgg tataagattt ttcgtctctc caagcgctag attatcagtg atccaccatt
    13141 tgaccaactg atccactcct actagcaaag cgctgaatat ccaataaata attaacaaaa
    13201 aatcaatcct tttctatttg tttataaata tcacgaggtc gtacgatccc gagcagatta
    13261 ccatcgggat tcccatcttt agtgataagt acggcttcca aagtcccttg ctcacgaaac
    13321 atcgcttcaa cctcgtaaat atgtgtttta gctggaacaa aatgataatt tgtgtacttt
    13381 ccgttttgga tgatcaaatc caacgctcgt ttatttgtta aatcaaccgg acctttttgg
    13441 ctttcttttg ctagccaaaa cccgatatta cgtaaagtaa tcaatgctac aaattttcct
    13501 ttattgtata acggaaattg tgaatacctt ttttctgcta ctatctttaa cagttcaagt
    13561 aacggaagat cttgttcaaa acctgttaca cgttttgcaa atcgaggcag cacggtttca
    13621 ggctgaatca gttcttgttc gatcagctgg attcgcttga ctgtccaatc atttggctct
    13681 gcaatgacaa aatcgactgc gatccgatca tggacgatcg cattccttaa ctgagcaagc
    13741 tgtaaaagat catcttcgta ttttctgatc attaattgtt ttctttgagc taggcgtctg
    13801 acaagttcgg taaatcccat atttcgtgca tttcccatct cgtctctcat ccatttttcg
    13861 attcgattga aactgattaa aaaaagatca gagtttgcca taattttcct ccttgatcaa
    13921 atatgcccgg ttttttatat agaaaaagaa cgcaatcaac acaataactg caaaaaaact
    13981 gaaaaataaa ttttgtagga aaacggcccc caacccagtt gctagaaata aaaacggata
    14041 gatttgaaga atcaagtaat tcattgtgat cacataagca ggctttgctt ctttcactgt
    14101 ataaacgggg ctttttagcc aataaaaggt gacctgcatc gtaaagggca cttccagatc
    14161 aagcgttatc ttttgttcat gactgagaac actgcgtttt tcattgttga ttcttagctg
    14221 aatcgagcta cccataccgt aaaaaccggt ttttcttata aatgtcgttt tcatcatacc
    14281 ctcctattat gctttagtat agctcaaaat acaataaaga aaaagaatga tcctagtgta
    14341 aacaaaatta attccctatc gtctaaaaaa agaagctgta gtcaaaatac agctccttcg
    14401 atggtagatt ataatcaagc ttaaaacaat cccaacactt ctccgtcatt tgtcacgtcc
    14461 atattcaatg cagcaggttt tttaggcaaa ccaggcatcg tcataacatc tccagtcaag
    14521 gctactaaaa atcctgcccc taattttggg atgatttcgc ggatcgtgat ggtgaaatct
    14581 tccggtgcac ctagcagtga aggatcatca gaaaatgaat actgtgtttt tgccatacaa
    14641 atcggcagtt tgtcccatcc attcttcgta aactcttcta tttgtttttg cgcttttttg
    14701 ctgaaaacaa caccatttcc tccgtaaatt ttcttgacga ttgtttctgt cttttcttgt
    14761 atcgtttctt ctaggcggta taatggcttg taattagctt cttttctatc aatcaatcgt
    14821 actacagctt ttgctaaatc aacgccgcct tctgctccat ctgcccatac actcgctctc
    14881 ttggcaagaa tcccctgatt ttcacacaag tgctccaaca gtgtgagttc attgtctgta
    14941 tctgttacga attcattgat tgcgacgatc acaggtagct gatattgttc catattacgt
    15001 atatggcgct ttaaatttgc aaaacctact ttcaaagcat caagattctc atttttaagt
    15061 tcatcttttt tcattccgcc atgcattttc aatgcacgga cagtggcgac aatcaccaca
    15121 gcatccggcg cttttttcaa gttaggtact ttgatatcta aaaatttctc tccgccaaga
    15181 tctgcaccaa atcctgcctc tgtgacaacg taatctgcta atctcaatgc tgtcttggta
    15241 gctaaaatac tattgcagcc atgagcgata ttggcaaacg gtcccccgtg gacaaaagct
    15301 ggtgttccgt aaattgtttg tacaagattc ggcttgattg catctttcag cagtaatgcc
    15361 agagcgcctt ctgctttcaa gtctccagca gtcacaggct gacggtcaaa tgtatagccg
    15421 acaacgatcc ttgcaagacg agcctttaaa tcttcaaggt ctgccgcaag acacaagata
    15481 gccatgattt cgctggcaac tgtgatatca aacccatctt ctcttggaac accttggatt
    15541 ggcccaccca agccaacaat tactttacgc agttcacgat cattgagatc gactacccgt
    15601 ttccaaatca ctcggcgggc atcgatgttc aattcatttc cttgatgaat atgattatct
    15661 aaaagagcag ataatgcatt attagctgtt gtgatagcat gcatgtcacc agtaaaatgc
    15721 aagttgatat cttccatagg caatacctgt gcatatcctc cgcctgcagc accacctttg
    15781 attcccatca caggaccaag tgagggttct cgcaatgcaa taacagattt tttattgatt
    15841 ttattgagcg cgtctcctaa ccctacagtg atcgttgact ttccttcgcc agctggtgtt
    15901 ggattgatgg aagtaactaa gatcaaatgc ccctcagcgt tatcctttga tcgattgatt
    15961 ccagaaaaat tgattttcgc cttatatttc ccatacagtt ccaaatcgtc ttccttaaga
    16021 tctaatttac tagccacttc aacaatcggt tctagctctg tttcttgtga aatttgcaga
    16081 tcattcttca tttgattacc ccttttcacg aactttattc aacaaaatat cttctttgtt
    16141 aagatatgcc aattatacct ttttttattc agaaaaaaaa cataaaatta caggttttta
    16201 atttatttga cgtacttata aatactcttt ataatgaagg ctatgatcag actttagaaa
    16261 ggtcgtgagt gctttgacca caggaattgt aaaatggttt gacaacaaaa aaggatacgg
    16321 ctttatcagt tatgatgaca cagaagaaat ttttgtacat tttactgcga ttgaagaaga
    16381 gggattcaaa actttggaag aaaatcaagt ggtcgaattt gagatcatcg aagggaatcg
    16441 aggaacacaa gctgcccatg tcaagaaagg caacgttcag actaccgata ctagtatgca
    16501 tgaatagata aacaaataga gtgagacgct aaacgggatg aaccgtacgt ctcactcttt
    16561 tcttttcctg taaaaattcg tttttttagg ttctgatttt taaaattagc tttttctttt
    16621 tttcgtataa ggataaggaa ttgattctga tttgggtccg tttttttcaa aatgatcaaa
    16681 aaggactact tgatcttttt ttcgcatcat gaaaacagat ggggctttct catcttgcaa
    16741 catcatttgc accccagtat tagttggcgt caaagatcga atctgtccga cagatatttc
    16801 tcggtctttc caaaaagcag cataacgtat aatcacagag tttgatttta aaataaaaga
    16861 acgccgcaaa cctagaaata gaaaaacaag aaaaatacct aaaacaaaat tgcttttcca
    16921 ataaggacct gtgttttcta aagaaagaat cagactaaaa aacagaatga tcaaggtcaa
    16981 tgaccaatag atgatcgtat gtgccagttc aggctgccat cgaatatttt ttttcattct
    17041 ctaacctctc accgattcag taatgatata atcttaccat aacattagtt tgaacaaaaa
    17101 ggagaatctc tttttatgtt aaaagtatac attgatgcct ctacaaaagg caaccctggt
    17161 cctagtggag gtggaatctt gattgtctat caaggtaaac aagaacagct atctatcccg
    17221 ctttctatag ggacaaacca tcaagcagag ttcgaagtct ttttaaaaac tttagaattt
    17281 ctaaagaaaa acgacttaca gaatgaaacg attttctgtt tttcagacag caaaacacta
    17341 gtttctacgg tcgacaaaaa cgcaacgaag aacgagctct tccttcccta ccttcaaaga
    17401 atccaagaac ttctatccga attttcttta ttgatcctgc aatggttgcc cgaaaacaaa
    17461 aataaaggag cagataatct tgcacgtcag gctctccata aacaattaaa agaaaaaaag
    17521 actctttaaa ttaaaaactt taaggagtct ttttatagtt tctcatttaa tataaaagat
    17581 aattgtgatc aaacgtaatt tccaattggt aatctccgat tttttcttct atttgactac
    17641 gtaagattct ttctagttct gtatcaacaa actggaattt ctcagggacc actacatcaa
    17701 attgcagcgt ttttccttga tgaattactc taaaatcatg aatattaagc cctgtacctg
    17761 tttcaagaag aatttgcttt aactgccgca agatattata gtatttttcg ttatcaacag
    17821 gcattggatc caagtgacag acaagttcca catccaaaga ctttttaaag tctttttcta
    17881 tcagatcaat aatcttatga gccacattca aattcattga ttcatctact tcgatgtgga
    17941 ctgatgcaaa gcgtttattg ggtccatagt tatgcaccaa taaatcatga tagccaagta
    18001 tatcatcgca agcatttaaa tgctcttcca tttcttcaat ctctgctggc gttgggcgac
    18061 ttcctaatag ttcgtataca aaatttcgaa gcatcgtcaa tccactataa aggatatata
    18121 atgctaaagc aaaacctata tatccatcaa tacgccatcc agttacccat tcgattgctg
    18181 ctgaaagcaa tacagccagt gtagtaaaga catcattata actatctgtt gctgtagctc
    18241 tcaatgtttc agaatcaatg gattgcgcaa taacctgata cattttgcct tgtgcaaatt
    18301 tcaaaaagat ggaaatgatt aggacaaaaa agacgatggg tgttaaagag acattttctg
    18361 gatggatgat tttttcagca gaagactcta aaaattgaaa tcctacataa gtaatgatga
    18421 tcgaaacaaa taacccactg atatattcga agcgttcgtg tccataagga tgttcctgat
    18481 ctgctggctt tgcagcaatt ttaaatccga tcaaagtcaa tatggatgaa gcagtatctg
    18541 atagactgtt cactgcatca gccataatgg agacacttcc tgaaaaaagc ccgatcatta
    18601 gcttagagac aaacaagatc agattcgtaa ccaatcctaa gatacccgct agtactcctt
    18661 tttttgtccg ctgttgcggt atttgtttct ttttttcttc ttgtttctta gccaagtttt
    18721 ttaccccttc tagtttgctt gttgctcagg taaaaagatg gagaggacga ttgttttcct
    18781 ctcctctctt cctttttcta tctatcgtgc gtcctattac tattaaaaga tggtcattat
    18841 tctggtttca acggtgttgc tgcaaataac gcttttgcta gtaatggtgt tatgattccc
    18901 gcacctattg cttctggtat cccattgatt gctgcaaccc ctgctaaggt tttaaataaa
    18961 agagcagagt ctacaccata ggcatctgcc gttgcacctg tataaaataa tcccatgaat
    19021 cctaacacta agactgtatt gaccaatgaa cccagtaccc ctgcaaaggc tgagctgatc
    19081 gttattgatt tatttttttg ataaatccag tggaaaacca agcctgctac taagccaaca
    19141 agtactctgg gaactaccga gataatcgga ttcgtgaaca ctaatgtatc aaatggcgtt
    19201 gttggcatgg cataggcacg aacaattgta gcgattcccc aaaacaagcc aatcaccatg
    19261 ccatcttttg ggcctaatac tactgcagca ataattactg tgatatggat aattgttaaa
    19321 ctaatgaatc ctaatggaat aaaccccagc caaggaacca tcgcttgtac gatgatgatt
    19381 gctaacaaga tcgcacgtaa gactagtcga aaggtcttat ttctactatt cattttttca
    19441 ccccttttta tttatactaa tcgtaaaaat ttctgtcact ttcgtcaact aaagaccatc
    19501 cgaatccgac catttgatta gatgcgccat ccaattgaaa aaagtatgag ggcaagactt
    19561 gtttgtcttc gcccccataa tcatgtttcg ttcaatgttt gaacttttgt ttttctgtca
    19621 aacgtaaacg ccctattgtt gcgcaaccca ttctttcgca gctttaagtg catctggaat
    19681 accagcagga tttttcccgc cagcttgtgc catgtctgga cgtccaccac cgccaccgcc
    19741 tactttcggt gcgattgctt taatcaagtc gccagctttt agtccagctt catttgattc
    19801 tttcgtcatt gccgctagca gactgacttt tccttcttgt tcgtttgcta aaacaagaat
    19861 atctgaaata tttttttgtt tccattggtc agctaattga cgcagctgat tcatatcttt
    19921 cacttttact tgtgctgcaa tgattgtttt tccattgatc gtttctggat ctttgaatac
    19981 atcaccagct tgttgattcg ctaacttgct agctaattgt tcattttcct tttgtagctc
    20041 tcttacttgc tgttgcaatt gttccgcttt tgaaacaact tctttcaatt gaggtgattt
    20101 aacaattgtt gctacttgtt ttagttgttt ttcttcttct tcaagtagtt cgtacgcttc
    20161 tttacttgtt acagcctcga ttcgtcgcac gcctgcacca attcctgatt cagaaacgat
    20221 tttgaagatt ccgatgtttt ctgtatttgc tacatgtgtg cctccgcaca gttcgattga
    20281 ccagccgcca atatttacta ctcgaacgtc atgtccgtat ttttcaccaa acaaagccat
    20341 tgcacccatg tttttggctg tatcgatatc tgtttcaatt gtttcaactg ggatagcttc
    20401 ccagattttt tcgttgacga ttctttccat ttcagctaat tcatctgaag tgatttgacc
    20461 aaagtgggta aagtcgaatc gtaaatgtcc aggagcgact aaagaaccag cttgatttgc
    20521 atgctcgcca agtacttctt tcaatgcctt gtgcagtaga tgggttgctg tatggttttt
    20581 gatgatccga ttgcgcatcg attgatcgat caatagatga taggtttgtc cttcactcat
    20641 tggtgctttt acttctacgg tgtgcataaa ttgaccatta ggcgcttttt gaacatctgt
    20701 aacaactgct acgatttcgc ctacttcatt catgatccat ccttgatccg ctacttgccc
    20761 acccatttct gcatagaaag gtgtttgatc aaagatcatt tgagcttcac cttctgcgac
    20821 ttcattcaaa agagactcac ctttaacgat gaccaataat tgactatctg attcttgttc
    20881 tgtgtatcca atgaatttac tttctacttt gatatctgtc aataaagcag attgtacccc
    20941 cattgatttt tcagtactgc gggcagagcg agcacgttcg cgttgtgcat ccatttcttt
    21001 ttcaaaacca gcatgatcaa ctttcagtcc ttcatcttct gccacctctt ccgttaattc
    21061 taccgggaag ccatatgtgt cgtatagttt gaagatgtct tttcctgcta gagtcgtccc
    21121 gttttcgttt tttacttctt ggatcacttg atttaagatt tccaaacctt cattgattgt
    21181 ttcattgaaa cgttcttctt ctgtacggat aactttttca ataaattctt tttgttgaag
    21241 tacttcagga taataactta ccatgatttc cccaacaaca gggactaaac gatataagaa
    21301 tgcttgttca atacctaatt ttttcccatg catgacagca cgacgaagca aacggcgtaa
    21361 gacataccct ctcccttcat tcgatggcaa tgcaccgtcg ccaatagcaa atgaaagggc
    21421 gcgaatatga tctgcgatta ctttgtaaga aatatctgtt actgggtttt caccatattt
    21481 tacttgtttg cttagctttt ctacggcgtg gataataggc ataaacaagt ctgtctcaaa
    21541 gttcgttgac gcatcttgaa cgattgagac catgcgttcc aatcccatcc ccgtatcaat
    21601 gtttttatga ggaagtggtt caaatgtgtt atcaggctta tggttgaatt ctgaaaatac
    21661 taagttccag atttctaaat aacgttcatt ttctcctcct ggatagtttt caggatcgtc
    21721 ttctgctaaa tcgttataag cttctccacg gtcatagaaa atttccgtat ccggaccaca
    21781 tgggcctgca ccgatatccc agaagttgtc ttctacatca ataatgtgat ctagtggcag
    21841 gccaacttct tcatgccaga tgcgttttgc ttctgtatct tttggataaa ctgttacata
    21901 aagtttttct ggatcgaatg ccatccattt tggatcagtc aaaaattccc atgcccagtg
    21961 gatggcttca tttttgaaat agtcaccaat cgaaaaatta cccagcatct caaacatcgt
    22021 atggtgccgt gctgttttac cgacattttc gatatcattt gttcgaatag atttttgggc
    22081 atttgtgatt cttggatttt ctggcacaac tgaaccatca aaatattttt tcaatgttgc
    22141 aacaccagag ttgatccaca ataaggttgg atcgttcacg gggatcaaag acgcactagg
    22201 ttcaatcgta tgtcctttag attggaagaa atcaagaaac atttgtcgaa cttgtgcact
    22261 acttaattgt ttcatgtcat tacctgcttt ctataaatta aatacaaaaa aacaccatca
    22321 cagccaagga cgaaaactcg cggtaccacc ttgattgcaa tgatgtgtaa tcattacctc
    22381 ttaaactcga taacgctgag ctgcgtcggt atttctacca aaatagaaaa agggagcaat
    22441 ttgattagtg actattcttt tttcagccaa gaaagaattt tctgtgagtc ttaaaaaaca
    22501 aatcatatcc ttttcgtact taattagtat acgcattcta ttgatcactg tcaatatctc
    22561 aatttttatt ttcatttcct tttggtcata ctccacgatt tttggtacga taagaataaa
    22621 ggaatcaata catctctgct attcatgaca aggaggaata tgatgaaaaa aatagttgga
    22681 gcggatgaat ggatacgcgc agctagctgc tttttgagat atcaagtttt tgtattagaa
    22741 caagggattc ctgcagaact agaattcgac ggaaaagata tcccgcaaca aacctattat
    22801 ctatttcttg atcaaaaaag accagtagct actttgcgtt atgaaaagaa aacacctact
    22861 tgcttcaagc cggaacgatt ttgcgtagca aaagaatacc gaggaaaagg gttaggcggt
    22921 cagttgcttg aagctgctga aaaacgggcg gtttcagaag gttgtcagac tagttatgta
    22981 gtagctgaaa tgacggctgt agatttttat gaaaaacaag ggtatcatac ttgcacagaa
    23041 ccttttattg aagatgaagt actttgtatc ggaatgaaaa aaagtctagc ttaaaaacaa
    23101 gagattgtga cacgcctttt gtcacaacct cttgtttttt atttatgtga atttttctta
    23161 tttttacgag cagtacggct ttgctgtctg cgttcgattt tgcgttcttt tttatttttt
    23221 tctgcaattg cccaatcgat ttttttctta taaccaggct tgatcttttt ctttttcttc
    23281 ttcacaagac caatcagcgt gggatctaat acatcttttg atttttctcg tttggcacgt
    23341 ctattacgat cgtatgtttt gacgatttcc ccgtttttga tttcttttgg ttcaaatgag
    23401 acgcccaatt tttctatcgc atcaatggct tgctcatcat ttggcgaata taacgtaatg
    23461 gccgttcctt ttaagccatt acgtccagta cgtccgacac gatggataaa gaagtctaga
    23521 tcttctggaa tttctgcatt aataacatga gagacacctt cgatatcgat ccctctagca
    23581 gctaaatcag tcgctacaac atattgatac tccagattct gtacttgtcg catcactcgt
    23641 tttctctctc ttggggtgat gtctccatga atctttgcta ctttcaagcc ttggtttttc
    23701 aagtaatcgg caatttcatc cacatgctgt tttgtattgg caaatacgat tgctaaataa
    23761 ggatgaccaa ttgtcagcag ttgataaatc atttgattct catctttacc ttttgttgaa
    23821 atcagccaat tttcaatgtc ttcagaaata actgcttttg gtttgatttc ttcaatgatt
    23881 ggattttcca tgtattttcg taagaatgga cgaagttttt ctggaatagt ggcagaaaaa
    23941 actaaaaact gcagcttttc tggtaatcga cttgcaattt ggtctacttc atttaaaaaa
    24001 cccatatcca atgtcatatc cgcttcatcg acaacaaatg caaatgcggt gtgaatcttc
    24061 agcgcttgtt cattcatcat gtctaatatt ctaccaggcg tgcctactac aacctgtggc
    24121 tgttggtgat gcagacgatt taattgacgc tgtttgtctg taccgccgac aaaattcgag
    24181 acacggatct ccggtgtaga aaatttagcg atttgtgtag ctgcttcata gatctgtgca
    24241 gctagttccc tacttggagc tgtaataaca acttgtactt cctctttttc tgcattgatt
    24301 ttttcaaaca aaggcaacaa aaacgtatgt gtttttcctg taccagtttg agattgccca
    24361 atgatatttt ttccttttgc aattaaagga atcaaacgtt cctggacctc tgtgggctgt
    24421 ttgaagccct ttgcggttaa cgcttcattg ataaacggtt tgaagcggaa tatatcaaaa
    24481 ttattcattc gtgcacctct tctctttaaa ttatcacgac tttggcatta taacacagac
    24541 aaagcgtttt tgctcagcta atttcgagaa tcatcatcct ttgtttggcc aatcgtgcta
    24601 acagtatatg tgtgactagc tttttcataa aacagaatcc gtttttttct caaaaatgat
    24661 agcaagatat ttaaaataaa aataccttgt agtaacaaga caccgagaga aatgaatact
    24721 gcactcatct gcaacaccgt attcgttgtc gaattcatga tttgctgaag caactgcccc
    24781 aaccagctta aaccataaaa aagaaagtac agatacccgt agcggatcat caatgctttt
    24841 aaagaaattc gttcgcggtc ttcttccacc aaacggatac gcacaatttt tttgccaagt
    24901 gtccatccat tggttataaa cggtacaatc ataaagtata cccctacttc taataaaagc
    24961 caccagatat gagaagtaac tgcccctgaa tcgaacgcaa ttttaaagaa aatcgccgct
    25021 accaaattaa taagagataa aaatgtccag tcgatcaaat aagcgaccaa gcgtcgagtc
    25081 aatgagacag tctttccttt ttcataagaa agttcatcca ttcgtcttct tgtaggaagc
    25141 ataaatgtaa ataacggcgt aacgacaaaa ccaatgattc cacccaatgt atttgtcact
    25201 aaatcattca catcaaataa gcggtatggt cttgggtaga taaaataaag tccacttagc
    25261 tgcgtcaatt caaaaaatag agaaaggcag aaactagcaa tcatcgtttt cataaaagaa
    25321 agccggaaat aatatcttaa ataaatgcca aacggtatga gtaataaaac attaaaagct
    25381 ggttccaaaa atacactctg cttcattgcc ggcaggtagg tactaggttc agacaagacc
    25441 aataccgtgt tgtctctgaa atgttgccat gaagcaaata aatgcagctc catctttggc
    25501 cctgtcattt gggcaaccgt ctcctttgcc ggaagtggta agatcaccaa aaaatacgca
    25561 cacaataaat aaaaaacgaa agaatacaaa ataactgccc ttgttactag aaatgttcca
    25621 tactttcgat attcataaat aaaaaaaaca gatgagatcg ctaaggctaa aaatggaaaa
    25681 acaatcagcg caaaacgaat cggttcgata taacttgcca tatgactcct ccttgattct
    25741 ttgtatctct cttatcttag cagtcctttg ctgtttcaag caagtaacga ggctattatt
    25801 tctcatttct ttcaaagtaa agtaagataa acacaaaggg ggtgtcgaga tgctgaatat
    25861 gtactttgtt tttggcgtac ctattttttt actgttcctt tatgctacga tcgcttatgt
    25921 aagaaaaaga actacaattc attatttagg atttattctc ctgattattt cgggtttcat
    25981 gctagtcttc aatcttcaga cttggcagca agcacttctt gaaatggata aaatgactcc
    26041 acatgctttg tctaaagtgc ttggctaccc tgtctatctg atttggcttc ccattttcat
    26101 ttctggttgc ttggttttat taaatattta tcgcggcgta cgccgaatag ttcagttacg
    26161 caaatcaaag taaaagacag aaatcggttg agcccgattt ctgtctttta ctttgctatt
    26221 tcatcgtaga caaaacgaca tttacgtgat caaaattaac gagtactttt gacatttctt
    26281 ccacgtactc ctcatttaaa taaaatattt tgtttggcag agaagtgcag ttctaataaa
    26341 attcgaatgt tttagctatc tacgttttaa atagagataa atcgggatac cagcaagtgt
    26401 caatactatc cccatcgaag ctaaaaaggt ctgagtgata agcgtgcttg atacgataaa
    26461 agtccctcca atgattgcta ctagtggaat aaacggatac attggaactt tgtatggtcg
    26521 aaacaaatca ggttcttttt tacgaagaag aatgacacca acaaagacca tcgtataaaa
    26581 aatccaaatg acaaagatca gcatatccgt tagtgtatca aaaccaccga tcatcatcat
    26641 gccaatagcg atgactagct ctaagatacc tgcggctaca ggaactttag ttttatcatg
    26701 aagtttagca aagagcttgc taaaaggcag gttgttttct tttgccataa cataagggag
    26761 acgcattcca gtaagggtat agccattgat ggttccataa acagaaatca agataccaac
    26821 tgtgaccagt cttccaccga agccgccaaa aatcattttt gcaacgtcac ttgccgcatt
    26881 gctgtttcct gcgactccgt cgatagatgc ggttcttaag aacaccgcat ttactaataa
    26941 ataaacgatc atgattccga tgattcctaa agaaattgct tttggcaaat ctttcgccgg
    27001 ctttttcaat tctcccgaaa tatttcctac gtgtatccaa ccgtcgtatg cgaacatcgt
    27061 tgctagcaag cccgctccca aggctgagaa aaatgaaaga ttttctcctg cttggatcgg
    27121 aaacagttgg aagtctactc cttcttgtcg gaacaaaccg aaaatgacga tcacaaacaa
    27181 aggaataagc ttgcagacaa gcgtaatcga ctgaaaagcc cctccagctt tagaacctaa
    27241 aaaattgatc aacaggatcg atacagcagc tgtaacagct acgggaacaa tcattgactg
    27301 ggaaagacca aatagattga cgaactgtgt accgaaaatg atcgacaagg ctgctacatt
    27361 tgctggaaaa taaatcacga cttgcgccca gcccaataaa aaagctgctg tatttccgta
    27421 aatccgttcg atatatttaa tcatccctcc tgtttctgga atagcagctg ccagttcagc
    27481 tcctgttagt cctgcacaga cagaaatcat accaccgaga aaccaagaaa acatatgcaa
    27541 actggcggat cctgtaactt ctgctacgct tgcggcttta aaaaataccc ctgcaccgat
    27601 cactgtgccc ataacagtcg ataaagcagt aaaaaatccc atcgtacgtt ttaattcatt
    27661 ttctattttt acttctttta ctggcgtggc ttgatattct tccatccctt gtcccctttc
    27721 ataccatagc caatcccaaa agtataaaaa actttttgga tgtggttctt gttttatacc
    27781 gtctctacca atttttcttt cagtttttcc aacatatcct ttgtcatcac atctaagtcg
    27841 tatttcggcg aaaagcccca ttcctcttgc gcacaagaaa catctagtga atttggccaa
    27901 gattctgcta ttgactgacg tacaggatcc acatcataat ccatttcaaa atctggtatg
    27961 attttttgaa tagatgcttt gatctcttcc gggtcaaaag acatcgccga tatattgaag
    28021 gcatttcgat gaaccaatcg atcaggatct gcattcatga gttttataat ggcatcgatc
    28081 gcatctggca tatacatcat atccattgat gtaccttgat caatgaaact agtgtacttt
    28141 ccttctttga ttgctgaata ataaatatcg actgcgtagt ctgtcgtacc gcctcctggc
    28201 aaggttgcat aagaaatcaa tccagggaaa cggacaccac gagtatctac gccatatttc
    28261 acataataat aatcacatag tagttctcca gctaccttcg ttaccccata catggttgta
    28321 ggacgctgaa gagtatcttg tggggtatga tcttttggag tatttggacc aaacgcgccg
    28381 attgaacttg gcgtaaaaaa tttcagctgg aattctctag cgacttccaa tgcattgacc
    28441 agcccagtca cgttcaaatt ccaggctaat tgtggttttg cttctgcaac agctgacaaa
    28501 agagcagcca aatgaatcaa tgtgtccact tgatattgtt ctgctatgct gcgcattttt
    28561 tcgtaatcca ttacatctag gatctcaaag atatcgtttt tctcaatgac ttcaggcatg
    28621 cgtatatctg tcgcaatgac attttcactg cctagttctt gacgtagccg tgtgatcaac
    28681 tctgaaccaa tttgtcctaa acatcctgtc accaatactt ttttcatgat gatcctccct
    28741 gatctttttt actactatct tcagatgatt aacaatatat gcgtgaacat gttctctaat
    28801 tttaaataga cgaaaaaact aaatttagtt aaataatttc catttctttt cctatttttt
    28861 gataaactgc tactgcttca tcaagcattt ccctcgtatg ttccgcagtc ggcatatttc
    28921 tgatcctgcc tgtcccaaga ggcacagttg gataaacgat tggtttagca taaatcccat
    28981 gttcgatcag cttcttagag aaagtctgag tcactttttc gtctcccaaa atgactggag
    29041 taataggtgt ttcagacata ccgatgttat agcctacttt tttcaattct tctttaaaat
    29101 aattagcatt ttcccaaact ttttcgacaa gttccggatg ttcttgcatc aaagtaatcg
    29161 aagccaatgc agctgcagct gcacctggag ttagcgaagt agaaaatagg aatggccgac
    29221 ttctagcttt caaccagtcg attagtgttt tcgatccagc tacataaccc ccaacaacac
    29281 ctatagcttt cgataacgtg cccatttgca tatcgatttt gtcgcttaat ccaaaatgct
    29341 tgacggttcc tgcacctttt ccagttacgc ctgagccgtg cgcatcatcg acgtaggtga
    29401 tcagtccata tttttcaaca atcggaataa tttccggcaa tcgtgcaatg tctccatcca
    29461 tggagaaaac accatctgtg atatacatga tttttttata tttcttactt tctatcgctt
    29521 cctttgcttt tttctcaagg tctttcatat cttgatgttt aattcggatg atttttgctc
    29581 cggataagcg gcaaccgtca ataatagatg catggtttaa ttcatccgat aagatagcat
    29641 cttcttttgt cattactgca gagatagcgc ccatattgca gttgaatcca gattgaaagg
    29701 cgattgctgc ttctgtccct ttgaattctg cgattttttc ttctaattgc tgatggattt
    29761 tcagcgaacc attgatcgtt cggactgctc ctgctcctac accataagtt tcggttgctt
    29821 caatacatgc ttttttcaat tcttcgcgat tggcaaatcc aagataattg ttggatgcta
    29881 gattgatcat cttttttccg tctacgatga tacaagcacc attttcactt tctagtatgt
    29941 cgatcgtatt gtataacccc ttttctttca gttgatccaa ttctttctct aaatacattg
    30001 ttagttcttt ggaagacatt gtctccacct ccatattcat ctgtcttgca gaacatacca
    30061 aacatatatc gtgtcattca tctacaaagc aagtatgcta tatttttgat aacgcttaca
    30121 ctgattatga gaattattta accacatttt ttatttacag tatcttttta ctttcaaatg
    30181 acttatatag tatgaacaat taccttttag ataatgatct attttactct acctgggcag
    30241 ataaatacaa cacaaccatt gatagaagcc ctttcgttaa aatcaattgt attctgaata
    30301 tttaatttat ttattgcata gaccaataaa aatacataaa atttccatct tcgtttatca
    30361 gcaattttta attttactct ctgaatattt cttaaaagtt ttacttgttg aattcgaaaa
    30421 aaggactgtg acaaaagtct tgtcacagtc ctattcttcc
//
LOCUS       DAYYKN010000017        29011 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000017 DAYYKN010000000
VERSION     DAYYKN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.017"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..632)
                     /locus_tag="ACHBB7_000654"
     CDS             complement(51..632)
                     /locus_tag="ACHBB7_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM9208629.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(638..1897)
                     /locus_tag="ACHBB7_000655"
     CDS             complement(638..1897)
                     /locus_tag="ACHBB7_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM9208630.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1899..2945)
                     /locus_tag="ACHBB7_000656"
     CDS             complement(1899..2945)
                     /locus_tag="ACHBB7_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208631.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3180..3455)
                     /locus_tag="ACHBB7_000657"
     CDS             complement(3180..3455)
                     /locus_tag="ACHBB7_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM9208632.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACHBB7_000658"
     CDS             complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACHBB7_000658"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM9208633.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACHBB7_000659"
     CDS             complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACHBB7_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM9208634.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACHBB7_000660"
     CDS             complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACHBB7_000660"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM9208635.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(7283..7918)
                     /locus_tag="ACHBB7_000661"
     CDS             complement(7283..7918)
                     /locus_tag="ACHBB7_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM9208636.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(7984..9387)
                     /locus_tag="ACHBB7_000662"
     CDS             complement(7984..9387)
                     /locus_tag="ACHBB7_000662"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM9208637.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(9374..10405)
                     /locus_tag="ACHBB7_000663"
     CDS             complement(9374..10405)
                     /locus_tag="ACHBB7_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9208638.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            10436..10657
                     /locus_tag="ACHBB7_000664"
     CDS             10436..10657
                     /locus_tag="ACHBB7_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM9208639.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(10822..11559)
                     /locus_tag="ACHBB7_000665"
     CDS             complement(10822..11559)
                     /locus_tag="ACHBB7_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM9208640.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(11712..13052)
                     /locus_tag="ACHBB7_000666"
     CDS             complement(11712..13052)
                     /locus_tag="ACHBB7_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9208641.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(13289..14812)
                     /locus_tag="ACHBB7_000667"
     CDS             complement(13289..14812)
                     /locus_tag="ACHBB7_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM9208642.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(15190..15801)
                     /locus_tag="ACHBB7_000668"
     CDS             complement(15190..15801)
                     /locus_tag="ACHBB7_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9208643.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(15924..16049)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(16131..16847)
                     /locus_tag="ACHBB7_000669"
     CDS             complement(16131..16847)
                     /locus_tag="ACHBB7_000669"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9208644.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(16853..17422)
                     /gene="scpB"
                     /locus_tag="ACHBB7_000670"
     CDS             complement(16853..17422)
                     /gene="scpB"
                     /locus_tag="ACHBB7_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM9208645.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(17406..18176)
                     /locus_tag="ACHBB7_000671"
     CDS             complement(17406..18176)
                     /locus_tag="ACHBB7_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM9208646.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(18173..18544)
                     /locus_tag="ACHBB7_000672"
     CDS             complement(18173..18544)
                     /locus_tag="ACHBB7_000672"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9208647.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(18563..19450)
                     /gene="xerD"
                     /locus_tag="ACHBB7_000673"
     CDS             complement(18563..19450)
                     /gene="xerD"
                     /locus_tag="ACHBB7_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM9208648.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(19546..20019)
                     /locus_tag="ACHBB7_000674"
     CDS             complement(19546..20019)
                     /locus_tag="ACHBB7_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9208649.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(20124..20987)
                     /locus_tag="ACHBB7_000675"
     CDS             complement(20124..20987)
                     /locus_tag="ACHBB7_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM9208650.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(21183..23282)
                     /locus_tag="ACHBB7_000676"
     CDS             complement(21183..23282)
                     /locus_tag="ACHBB7_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM9208651.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(23502..24611)
                     /gene="rpoD"
                     /locus_tag="ACHBB7_000677"
     CDS             complement(23502..24611)
                     /gene="rpoD"
                     /locus_tag="ACHBB7_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM9208652.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(24635..26476)
                     /gene="dnaG"
                     /locus_tag="ACHBB7_000678"
     CDS             complement(24635..26476)
                     /gene="dnaG"
                     /locus_tag="ACHBB7_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM9208653.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(26658..28973)
                     /locus_tag="ACHBB7_000679"
     CDS             complement(26658..28973)
                     /locus_tag="ACHBB7_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9208654.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
BASE COUNT         7941 a         6239 c         4905 g         9926 t
ORIGIN      
        1 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
       61 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
      121 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
      181 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
      241 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
      301 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
      361 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
      421 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
      481 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
      541 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
      601 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
      661 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
      721 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
      781 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
      841 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
      901 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
      961 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     1021 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     1081 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     1141 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     1201 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     1261 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     1321 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     1381 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     1441 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     1501 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     1561 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     1621 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     1681 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     1741 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     1801 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     1861 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     1921 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     1981 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     2041 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     2101 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     2161 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     2221 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     2281 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     2341 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     2401 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     2461 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     2521 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     2581 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     2641 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     2701 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     2761 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     2821 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     2881 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     2941 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     3001 agataagagc aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg
     3061 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     3121 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     3181 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     3241 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     3301 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     3361 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     3421 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     3481 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     3541 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     3601 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     3661 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     3721 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     3781 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     3841 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     3901 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     3961 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     4021 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     4081 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     4141 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     4201 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     4261 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     4321 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     4381 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     4441 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     4501 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     4561 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     4621 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     4681 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     4741 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     4801 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     4861 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     4921 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     4981 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     5041 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     5101 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     5161 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     5221 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     5281 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     5341 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     5401 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     5461 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     5521 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     5581 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     5641 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     5701 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     5761 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     5821 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     5881 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     5941 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     6001 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     6061 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     6121 cgttttgaaa gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact
     6181 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     6241 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     6301 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     6361 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     6421 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     6481 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     6541 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
     6601 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
     6661 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
     6721 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
     6781 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
     6841 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
     6901 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
     6961 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
     7021 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
     7081 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
     7141 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
     7201 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
     7261 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
     7321 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
     7381 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
     7441 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
     7501 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
     7561 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
     7621 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
     7681 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
     7741 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
     7801 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
     7861 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
     7921 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
     7981 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgttcccc aatgaagttg
     8041 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
     8101 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
     8161 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
     8221 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
     8281 catccatttt ttttgcagct catcaaaggg agcttgctct gtggcatatt ccaaataaag
     8341 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
     8401 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
     8461 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
     8521 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
     8581 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
     8641 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
     8701 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
     8761 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
     8821 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
     8881 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
     8941 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
     9001 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
     9061 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
     9121 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
     9181 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
     9241 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
     9301 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
     9361 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
     9421 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
     9481 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
     9541 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
     9601 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
     9661 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
     9721 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
     9781 gttgcagata aaccgctgac tcatcattgt tttttgccag ctgataagga agcagccctg
     9841 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
     9901 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
     9961 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    10021 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    10081 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    10141 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    10201 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    10261 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    10321 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    10381 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    10441 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    10501 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    10561 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    10621 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    10681 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    10741 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    10801 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    10861 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    10921 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    10981 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    11041 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    11101 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    11161 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    11221 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    11281 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    11341 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    11401 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    11461 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    11521 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    11581 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    11641 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    11701 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    11761 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    11821 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    11881 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    11941 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    12001 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    12061 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    12121 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    12181 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    12241 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    12301 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    12361 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    12421 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    12481 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    12541 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    12601 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    12661 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    12721 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    12781 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    12841 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    12901 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    12961 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    13021 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    13081 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    13141 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    13201 agctattgac ttatccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    13261 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    13321 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    13381 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    13441 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    13501 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    13561 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    13621 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    13681 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    13741 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    13801 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    13861 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    13921 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    13981 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    14041 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    14101 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    14161 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    14221 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    14281 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    14341 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    14401 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    14461 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    14521 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    14581 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    14641 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    14701 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    14761 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    14821 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    14881 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    14941 cctattccct cctctttttt caggttttta aagctttctt ctttttcatt gtagcgtttc
    15001 ctttttttta taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa
    15061 gaattgattg atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt
    15121 tacattattg catgtcttgt atttttttcc aaaaaaaaag accaagaaat ttcttgatct
    15181 ttttgatatt tatttgactg ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa
    15241 ccatggaaga agttgcgcat gcagcactaa aaagacagca ctcacgatga atcctttgat
    15301 caagttgaaa ggaacgatcc caatcagtac atagtttgct aaagacatgc ccagcatctg
    15361 tgttgcgttc agccctaaaa acattaagta aagaggcgta ataacgaaat aatttgcgat
    15421 actcataaaa atagttagag cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc
    15481 tttatgctgt ttctttacac taaagaaata ataaatcggg aatgtaaaga tcgttgttga
    15541 taagaaacta gcagtgtctc ctaccaaatt atccggagaa aaacctgtag tcagcagatg
    15601 taatactgaa cgcaagaacg ctgttgcaac tccggccgct ggaccgaata agaacatgct
    15661 gatcaatacc gagatatcac taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg
    15721 aaaagcaaaa tactgtaaaa ctaatgcgat cgcagcaaac atcgctactg caaccatttt
    15781 ctgtacacgg gtgttcttca tttgaaatcc tcctgttagg actcctcttc agcgaacttt
    15841 ttgacgtttc tctccaaatg aaaaaactct attttccagg gaaaatagag ttagagttat
    15901 ttgatcaagc gtcgtcaaat aagctctatc ttctttatcc agactatact gtcggtatcg
    15961 gaatttcacc gattcagcta tttttttgca aaataggtcg tggactataa ccaccggtcg
    16021 ggaatttcac cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat
    16081 acatatctat tcaaaaaacg caacatctaa cacttataaa aagtaagtgt tcattttgag
    16141 tggttcagca aactgctgac ttcttttttg ttcagttcac ggtaatcccc aggacgtagg
    16201 ccttgtaaag tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact
    16261 gcttgaagca tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata
    16321 ctgtggtttg tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt
    16381 ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt
    16441 acctttgcaa cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa
    16501 tctccatcat ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga
    16561 tagatacgtt cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct
    16621 gcaacagcag aaataactcc tttaggtttg tacaataaat agtaaccgta ttcttcttga
    16681 taaattggga cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc
    16741 cttacaactt gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga
    16801 cgagaagtga tgccagcgtg ggcaattact ttctgcaatc tctccattac aattattctc
    16861 ctttattcat tccatcaaag aatagatctg gtgacacgtc ttcttcaagt gaaatttcca
    16921 tttcttgtgt atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc
    16981 cagttgttcc atataatatc ggccgacctg gtccttctac tctacctttt tcttcaatca
    17041 gtttatgggc agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga
    17101 tttcagctct tgtgattggc tgtttataag cgatgattgc taaggtttcg acggcggctt
    17161 gagataatgt gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt
    17221 tagtcgttaa tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt
    17281 gagtgtaaga ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta
    17341 acagtccgct taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt
    17401 caagttcact taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat
    17461 tcatagtttt ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc
    17521 ataaaagtag tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag
    17581 ccatgcgcat ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg
    17641 tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc
    17701 tgcatggcac ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca
    17761 tccgctaacc catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct
    17821 ttttctgtca gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc
    17881 aattcttctc gaggatcttc ttcccataat tcttcatcgt ctgtgacgaa ttcttggaca
    17941 ggaagcaatg tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct
    18001 gcaacagcta gctcgagtgt tttcattgta tggatataat tcatatattg ctccgtgatc
    18061 gtagcgatcg gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga
    18121 tcgagaggac cctcgaatac atctaattta atattgatct ccgtcaactc attcactctt
    18181 tcttcttttt ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga
    18241 tggagacatc cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac
    18301 cgcgataaga cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt
    18361 aaataccaat aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat
    18421 aatcgtcttc agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt
    18481 tcttatggaa actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta
    18541 acatcttctc accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca
    18601 ttcttttctt cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca
    18661 attcctgtac tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc
    18721 gcagcgtatg tggtgttact tctttgtaaa tcccagcctc tcgtactaac tgcttcagat
    18781 ttttccagat tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt
    18841 cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga
    18901 tcgcataatc acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca
    18961 gtccaagaga aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc
    19021 cagtagcata catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat
    19081 caggtgcttc aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg
    19141 ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta
    19201 aaaactgatg gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt
    19261 tttcttcatg caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg
    19321 aagcaatttt ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt
    19381 gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt
    19441 gctctttcat aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga
    19501 tagtcttttt ccataatctg agcaaagaaa aagccgtcta gacactcaga cggactggtt
    19561 tcttttttct ttttcatagc attcctgaca gattccatgg aacgttagcc gatgatcttt
    19621 tactaaaaaa tgataacgtt cttctactac ttgctccact tcaagtaaaa gatcctcttc
    19681 tacttcctcg atcgtgccgc agttcaagca tagtaaatga tgatggaaat gtttggcgcc
    19741 ttcttttctc aaatcataac gggctaatcc atcattaaaa cttactttat cgacaatttt
    19801 caattctgtc aagatttcaa gggttcgata aacagtagca agtccaattt ccggactctt
    19861 ttttttcact aaaaagaaga tttcttctgc agataaatgc tctttctcat tttccaaaag
    19921 cacaagcaga gtagcttctc tttgcggtgt cagcttaaac cccgcttcat gtagctgctg
    19981 ttttgttttt ttcatcgccg aaatagcatt catgaccatt tactcaaccc caacaattta
    20041 taataattac aaataaaaaa agatataaca ctatgttaat taaaattatt ataagctagt
    20101 taagaaaaac atgcaaggga ttctcaatta gattttttga ctagttttgt cacaccgttt
    20161 tcttgtgtaa tcaatccttg tttcaaaaga ttacctaatg cgcgcttaaa tgctcctttg
    20221 ctgataccaa aagctttcat gatttcttct ggatccgatt tatctgtaaa gttgatttgc
    20281 tgatctgcag aacgttctaa aatggttaaa atcatttgtg catcatctga tatagcttca
    20341 tatgctcttg gcttcaatga aagattcagt acaccgtctg gtcggacacc aatcactcga
    20401 ccttttatct gctcgcctag acgaggttca ttatatcgtt cagaaggatg gataaaccca
    20461 agatagaaat cttccgttaa aacgtacgta cctgttaatt tcaagcggta tgctgttcct
    20521 atcacatctt tgtttttcat ttcttctgtt ccatatttgc ttaaagattt gaatattttt
    20581 tcatctgcta aactgcccca aatacggtct tttgcatcaa ctctaaggga aatcatcaag
    20641 cgatcccctt ttttaggcca tagctctgtc attgtcggca attcatcaag agatacgaca
    20701 aagtctttat ctggaagacc gatatcgaca aacgcaccta aatcttttcg tgtagcagta
    20761 acgattccaa aagcgtaatg accgatacgg cttttaggaa tttgtgtagt gatccgattt
    20821 tcatgttttt gattttggta accgaatcct tcaactgctt caccgatttt gtgttcacct
    20881 tctgatttgc ttaagccaaa ggtttgtccg tttttttgga caaagtagtt tttttcattt
    20941 tcatcaataa taatacctgt aaatatagtt gctaataaat cgttcatttt tttctccctc
    21001 tatagtgaaa ggttttctta atacatttct ttgctatcat aacataatgt tatctacttg
    21061 aatagctgag ccttggtttg gaaaggtgaa aaaaggagta tgacaacagt tgtgtcatac
    21121 tccccttttt cgaataaacg gtgttaaacg gcaggctcac agcctctttc atttgcctaa
    21181 aattaattgc tcccatttcc tccgaaccat ccagttttac agttgatggt tacgatgtaa
    21241 aatggcaaag cactttctcc aatgcctttc atcaatgcag attttgttga agtggcatat
    21301 agattataat agccttcccc attttcgatt ctggcttttt ctaatctgta acctccttca
    21361 acgatatatc cacgttggat acatgtagca atcactttgc tttggacacc tggtgcccac
    21421 gtccatgctg gattagatgc atcggcaata tcacttgctc tggcagattt gattggctga
    21481 ttcgctgagt ttgaagagga aggggtcgag gatgccgctt cttgctgtgc tgcttgtgcg
    21541 accgcagctt gttgagcggc ttgagctgca gctgctgctt gagcggcttg ttttgcttgt
    21601 tcttcagctg cttttttcgc ttcttctgct tttttctttt cctcttcttc ttttttctct
    21661 ttatcagtga gcatcttatc tacttgtttc aattgttcat tcaactgagc agatagatct
    21721 gtatttttca ctttttctac tgcttctttt gccgtcttgt attggtcgcg tgttgctgaa
    21781 tccactactt ttccgtcttt aaagactata gctgtttttt ctttagctgt ttgcaattca
    21841 gcatattgat ttttagcttc tgtttgtgct tgttgaatca attttccaaa atctgtgtta
    21901 tccgtttcga ttgcttggat ctctttatct gctttcaact tgatgtcatc tgctagatga
    21961 tcatcgacga tggcaggtgc tatgaaccac tcattcactt tttggatatc tcggatctta
    22021 gattggatat cttggtaagt tgtttctaac tctgaatatc ctttttcatt cttaagactg
    22081 gataaatcat tttttagttt tgataaatct tgatgaatca tatctgcacg aataaattga
    22141 tggtgatcat ctgtataaaa ggcagcaagt ttctcatgaa gttcttccat ttgttgctgt
    22201 ctttttgctt ctgctgctac ttcggcttct actttttgat gatggtcata ataagcccag
    22261 cttcctccac cgagtagaag tactgctgct aaaacgagat aaggtacttt ttttgatttt
    22321 ttcttttgcg ctttatctgg atctgttttt tctaagtttg attcttgatg tgttttttca
    22381 gtatctggca cgattgttat tgatttcgct tctgcttcaa acagcttagg ctggttctct
    22441 tctttttcta tttttgcttc aagatcagta ccctctttgc ttacagattg attttcagca
    22501 tcggattcac tatttatttc atcgggctct gttgactttt tgtctgtgtt ttcgagtatt
    22561 tcttttttct ctgcttccgg cagtttattc gttttttcct tttctaattg tttttcagta
    22621 aatacttcag ttttttctgt gatatccgtt tttacgtctt ttatttttac tgtttcttta
    22681 cctgtggcgt tttctctatc agagtctttt tcagtttctt gttccttttc tggctctact
    22741 ttgttttcct gtgcaatttt ttcactgatc attttgagca gctctttcgc gtgctcttct
    22801 tttgtatctt cacgatgtgc tttgatataa gcggatagta tcgaattttc ttcctcatta
    22861 tcgacgattt ctttcagttc agctactctt cttttttgtt ttgattcaag cgtttcttct
    22921 tgattctttc ctgattcttc cgaagtctca tgaacagttg ctttcttctg ttcatttttc
    22981 tctatattag ttacttgatc gtgagcaggt ggttcttcgt cttctggttc tgctttttcg
    23041 aataattcca ttacagattc tagaggtaaa tctttcaatt ccgaccattc gattggatcg
    23101 tttaattctg tatcaggaaa gactgcatct tcagttgatt tatcctgcat agctgcatct
    23161 gtatttgttt cttcaattga ttcttttatt tgctgatctt ctgcagttat ttgatgaccg
    23221 cagtctggac agatagtatt ctgctccatt tttttcgatc cgcaatttgg acattttttc
    23281 atcacttagc gatcggctcc tttactaggt ttacgctacc atttaatcta tcatattcta
    23341 tacgtcacgc caatagctta tttttaggat ttacttaaaa ataatgaatg aatttccttc
    23401 ctatagaaaa aaaagcttga gactgcgtag tctcaagctt tttttctttt aaaataaacc
    23461 aaacagtttt actgatttct cttctggtca atagtccacg cttattctaa gaaatctttt
    23521 aattgttttg aacgagaagg atggcgcaat tttcttaatg ctttagcctc gatctgacga
    23581 atacgttccc gagtaacacc aaatactttc cctacttctt ccaatgttct cgtccggcca
    23641 tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc gatcagtcaa tgtatctagt
    23701 acatcttcca attgttcttt tagtaactca taagctgcgt gttctgctgg acttgttgct
    23761 tcctggtctt ctatgaaatc acctaagtga gagtcatctt cttcaccgat tggcgtttca
    23821 agggaaacag gttcttgcgc gattttcaag atttctcgga ctttttctgt tggaagatcc
    23881 atttccgcac cgatttcttc tggcgtaggt tctcttccaa ggtcttgcag caattgacgt
    23941 tggatacgta tcaatttatt aattgtttca accatatgaa ctggaatacg gatcgtacgt
    24001 gcttggtcag cgattgctcg agtgatggct tgacgaatcc accatgtagc ataggttgaa
    24061 aacttaaatc ctttgcggta gtcaaatttt tcaactgcct tcatcagtcc catgtttcct
    24121 tcttggatca agtcaaggaa ctgcatgcct cgtcctacat aacgtttagc gatacttact
    24181 actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt cttggtcgcc ttcttcgatt
    24241 ttcaatgcta acgctacttc ttcttccgca gttaaaaggg agacacggcc aatttctttc
    24301 agatacatac gaaccgggtc attgattttt acaccggttg gcgcagaaag atcttctgtt
    24361 tttgcttgtt cagctttttt ctcagcactc ttcaagctgt gaattgatgg atcaccattt
    24421 tcatccacca cactgatacc tgcgtcttca atcttttgga tcaatttgtc catttcatct
    24481 gcattcaaag tgaatggtgt agcaagctga ttggataaat catcatatac aacttgtcgt
    24541 ttgggtttgt tttctttgat gaaagctgct actgctgctt catacgtttt tttatctgtt
    24601 ccatttgcca taaaagaagg ccccccttga gtagctattt ggcttgtttt aactgtttcg
    24661 ttaaattgat gatttcaatc gtcaattcaa gctcaagctg tttgtttcca gttcgtctgg
    24721 cttcttgctg catttgtttt tttaatgtta tttcatcagc taagccagat ttgctgatca
    24781 catctagcaa gtcttggatt tctcgtggcg aactttcttc tgccaccggt aaatattcga
    24841 tttccaccac caaccgcctt aaattttcat cttgcaaaaa gtctaaaaac tctgcaaggt
    24901 tgaattcata atgttgtgag acatatgcat cgaataaaaa ataaacttct gcatatgcat
    24961 catgaataaa ctgaaattct cgttctttta aaatattacg gacagaaact tctttaaatg
    25021 cacgatataa tagaatctgc tctgcctttt ctacttgcgt caaaggtctc tttttccgtg
    25081 cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg aggttcttga cgattttgct
    25141 gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt ctctctgccg atctgaaact
    25201 ctgttgacag ttgagataga tacatatctt gttcgattgg tgaaacaact cggctaagtt
    25261 caactagtaa ttcttcgagg tattcgatct tgtctttttc attatctaaa tttttccctt
    25321 gttttaaata acgtgattta aaagtaaaga ctgtttcgcg cccatgagcc actaattctt
    25381 ggaaagatgc ttctccatac ttacggagat attcatcagg atcaagcttt tctggaacga
    25441 ctacgacaga aagtgctaat cgactgtttt gccgaagcaa ttcaatccct cgattggttg
    25501 cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa ttctttcgtc agcttttcca
    25561 tttgttggat ctgttgagct gttagacttg tccccataga agctaatcca gttttgatac
    25621 cagcctgcca tgcagcaatc acatccataa acccttcgaa caacaaaatt tctccgtttt
    25681 tccggatatc gcttcttgct ttatcaagat taaataaaac ttgtctttta ttgaacagtt
    25741 ctgtttctgg actattcaag tatttgggct gatctttttc ctgccctttt tcaggtgcaa
    25801 gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt acgaagtgga aacatcactc
    25861 tttgataaaa acgatcagac atacggccat catcatgcgt gacaaacaat ccagagcgtt
    25921 ctaacaattc atttgaaatt gattcattct tgaatacttg ctgaagaaaa gaacgctcat
    25981 taggtgcaaa acctatgttg aattcttcaa ttaattctaa agacaaccca cgactttgca
    26041 aatattctaa agctggctgt ccagcttttg tatgcaatag catgtgatga tagatttccg
    26101 ctgctttttc atgagcagca atcagtttcc ccgtagcaga ttcaggattt tggacagcta
    26161 tagtacgctg acgccattga tcttctaacg gtatctgttc gaagtctgcc acttttacca
    26221 ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa agtaaacacg tttccgcctt
    26281 taccacaacc aaagcaatga aaaatctgct tgtcttctgc aacagaaaaa gacggtgtac
    26341 gttcttcatg aaaaggacac agccccaagt aatttttacc agactttttc aactggacat
    26401 attgtccaat cacctcaacg atattcgttt gactgcgtac tgtttcgata acctcttgag
    26461 gaatccgttg agccattttt tcaccccctg ctctattgat atcttttggg tttaacagaa
    26521 gatatttcag gagaataacc ggtaaaaaat cgcactaact gaaatcagtg cgattttaaa
    26581 ggacacaatt atacatgttt gattgtatca tatttcattg ctttttcaag tataacacat
    26641 ttctatctca ttaagcatca atttgctact cttttcagta actgattttg aatatcagcc
    26701 ttccagtaca atactcgaag aacaatattt acgagcatga atacaaagaa gtatcctacg
    26761 ctctctccca accctaagtt tacttccaaa attccatgga acagcattgc agctacatat
    26821 atccctacga tcgtcgttac tgctaggaag atgcgcaatg ggttgaaggg ataacaagct
    26881 tttacaactg ccatgcaact gatcccaatc agcaagtaat acatcaaagt agtagtttca
    26941 gcatttcccc atgcgaatgt ttttccaagt agatacaccg caatgatatt cactactaca
    27001 agcaaggcat ttggtaaagc attaataagt gctgtaggca aataacggta agtgattttt
    27061 cttttatctt gttcaaaaga taaaaagaag gaaggatacc cttcaatcgc taaatcaatc
    27121 agcgtgattt ggatagggat aaacggaaat ccaattgctg taacggcaca tataatcgat
    27181 aaaataaatg aataaatcgt tttgataaaa aagatgcctg agactttcgt cacattgttg
    27241 accactcggc gtccttcaaa caggacttct ggcaatgtag taaaatcaga gtccaacaat
    27301 accagattcg caatctgacg tgttgctcca tcaccttctg ccatagcaat gctgcaatct
    27361 gcttctctta acgccaaaac atcattcaca ccgtcgcctg tcattgcaac cgttctgccg
    27421 ctttctttca attcatttac cagcaatttc ttttgctgcg gactcactcg tccaaaaacc
    27481 gtatagcgat gagcagcttc tcgcacttcc atttcttcag taatttgaga aagatcgaca
    27541 tacgcatcat aattcggtaa tcctgcacgt ctagcgatat tggagacagt aaaaggattg
    27601 tctccagaaa tcactttcag atccacacct tcctcatgaa gataagctag tgtttcttga
    27661 gcgttttttc ggattggatc atcgatttca aacagagcaa ccggctcaac aaatgctggt
    27721 ttttcttcgt tcaattgctg atttggagcg atccccaaca taagtacgcg atagccattc
    27781 gcttgggctg tttttagact gtccggaagc cgatcttctt ccaccaaacg ttcaggcgct
    27841 cctacgtaca cgactcccag ttctggaaat tccatcgcgc cccatttgcg atcagaagaa
    27901 aatgcgactt tgtttgtcac atcataccga ttagaaagct caaaataatc tctcatagcc
    27961 cgcatagtga tgttgttatc actgctttct gccagatacg tccccataat ttctgggatg
    28021 gctttctcgt acagcggatg tagtgcttct acccgctgga tactcatttt cccttcagta
    28081 atggtccctg ttttatccaa gcataatgta tcgacatggg ccagcgtttc aattgaatac
    28141 atgtcttgaa caaggattcg tttttttgcc agtttggtta cccctgtcgt taatgcaata
    28201 cttattagca ataccaaacc ctttggaagc atgcctaaca aagctgctgc agatgcaata
    28261 acagaaattt tgatagaagc atcgcggatg aacagtgctt ccaaaaatag tatgatcccc
    28321 aaaggaataa tcacgaaact agtgaatttg gatactttgc gaatcgaatc tacaagttca
    28381 gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc ggacagcata attatctgca
    28441 cctactcgga taacttcagc catgacagaa ccgcttgtca aaaagcttcc agaaagcaat
    28501 tcagctccag tttctttgac aatcaaatca gattcgcctg tcaaaagagc ttcatttgct
    28561 tctgcccgtc cagatataac ctgtaaatcg gctggtatct gttctcctgc tccgattttt
    28621 accaaatctc ctaataccag ctctttagta gaaatcgatt gttctttacc gtcccggatc
    28681 actagtactt ctgattggga aacaatcgat aatttccgta caagatttct tgcatgtatt
    28741 tcttgataaa ttccaataac gatattgacg acaataattg ctaaatacgc catgttggaa
    28801 tacgcaccta caaataacag acaaatccca ataccaaagt tcagaaaatt gaataacgtc
    28861 aatatattat cgctgaatat ttgttttgta ctttttgatg catcttcctg gtagtcattt
    28921 tgctggccag aagccatttt ctgaactacc tcttgctgtg taagtccatt cattttcttt
    28981 ttctccattt tccgccaact ttcaaaaata a
//
LOCUS       DAYYKN010000018        28626 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000018 DAYYKN010000000
VERSION     DAYYKN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.018"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..906
                     /locus_tag="ACHBB7_000680"
     CDS             199..906
                     /locus_tag="ACHBB7_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFM9208655.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            899..1027
                     /locus_tag="ACHBB7_000681"
     CDS             899..1027
                     /locus_tag="ACHBB7_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208656.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            1085..1426
                     /locus_tag="ACHBB7_000682"
     CDS             1085..1426
                     /locus_tag="ACHBB7_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208657.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            1419..2165
                     /locus_tag="ACHBB7_000683"
     CDS             1419..2165
                     /locus_tag="ACHBB7_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM9208658.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            2171..2857
                     /locus_tag="ACHBB7_000684"
     CDS             2171..2857
                     /locus_tag="ACHBB7_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM9208659.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            2864..3697
                     /locus_tag="ACHBB7_000685"
     CDS             2864..3697
                     /locus_tag="ACHBB7_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM9208660.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            3714..4565
                     /locus_tag="ACHBB7_000686"
     CDS             3714..4565
                     /locus_tag="ACHBB7_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9208661.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            4562..4921
                     /locus_tag="ACHBB7_000687"
     CDS             4562..4921
                     /locus_tag="ACHBB7_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM9208662.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            4936..5097
                     /locus_tag="ACHBB7_000688"
     CDS             4936..5097
                     /locus_tag="ACHBB7_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9208663.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            5094..5399
                     /locus_tag="ACHBB7_000689"
     CDS             5094..5399
                     /locus_tag="ACHBB7_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208664.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            5399..5716
                     /locus_tag="ACHBB7_000690"
     CDS             5399..5716
                     /locus_tag="ACHBB7_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM9208665.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            5713..6099
                     /locus_tag="ACHBB7_000691"
     CDS             5713..6099
                     /locus_tag="ACHBB7_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM9208666.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            6096..6431
                     /locus_tag="ACHBB7_000692"
     CDS             6096..6431
                     /locus_tag="ACHBB7_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208667.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            6431..6565
                     /locus_tag="ACHBB7_000693"
     CDS             6431..6565
                     /locus_tag="ACHBB7_000693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208668.1"
                     /translation="MKDWLEEASALVGCFINCAFISVIIALVGFVSLKVIILLWQLIF
                     "
     gene            6577..7146
                     /locus_tag="ACHBB7_000694"
     CDS             6577..7146
                     /locus_tag="ACHBB7_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM9208669.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            7143..7316
                     /locus_tag="ACHBB7_000695"
     CDS             7143..7316
                     /locus_tag="ACHBB7_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208670.1"
                     /translation="MSRQIMLHIPDGTKAMTITIVAAQKDGGLALKTKSIDTKQILEG
                     RDVEIEIDEEEAE"
     gene            7313..7618
                     /locus_tag="ACHBB7_000696"
     CDS             7313..7618
                     /locus_tag="ACHBB7_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208671.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            7642..7839
                     /locus_tag="ACHBB7_000697"
     CDS             7642..7839
                     /locus_tag="ACHBB7_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM9208672.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            7836..8042
                     /locus_tag="ACHBB7_000698"
     CDS             7836..8042
                     /locus_tag="ACHBB7_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208673.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            8042..8338
                     /locus_tag="ACHBB7_000699"
     CDS             8042..8338
                     /locus_tag="ACHBB7_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208674.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
     gene            8414..8827
                     /locus_tag="ACHBB7_000700"
     CDS             8414..8827
                     /locus_tag="ACHBB7_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM9208675.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            9511..9678
                     /locus_tag="ACHBB7_000701"
     CDS             9511..9678
                     /locus_tag="ACHBB7_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208676.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            9704..10048
                     /locus_tag="ACHBB7_000702"
     CDS             9704..10048
                     /locus_tag="ACHBB7_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM9208677.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            10053..10334
                     /locus_tag="ACHBB7_000703"
     CDS             10053..10334
                     /locus_tag="ACHBB7_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208678.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVDIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            10437..10751
                     /locus_tag="ACHBB7_000704"
     CDS             10437..10751
                     /locus_tag="ACHBB7_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM9208679.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            10729..12423
                     /locus_tag="ACHBB7_000705"
     CDS             10729..12423
                     /locus_tag="ACHBB7_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9208680.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            12443..13621
                     /locus_tag="ACHBB7_000706"
     CDS             12443..13621
                     /locus_tag="ACHBB7_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9208681.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            13584..14270
                     /locus_tag="ACHBB7_000707"
     CDS             13584..14270
                     /locus_tag="ACHBB7_000707"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM9208682.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            14270..15430
                     /locus_tag="ACHBB7_000708"
     CDS             14270..15430
                     /locus_tag="ACHBB7_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9208683.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            15440..16315
                     /locus_tag="ACHBB7_000709"
     CDS             15440..16315
                     /locus_tag="ACHBB7_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208684.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            16312..16623
                     /locus_tag="ACHBB7_000710"
     CDS             16312..16623
                     /locus_tag="ACHBB7_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208685.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            16613..16966
                     /locus_tag="ACHBB7_000711"
     CDS             16613..16966
                     /locus_tag="ACHBB7_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9208686.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            16956..17357
                     /locus_tag="ACHBB7_000712"
     CDS             16956..17357
                     /locus_tag="ACHBB7_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208687.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            17350..17754
                     /locus_tag="ACHBB7_000713"
     CDS             17350..17754
                     /locus_tag="ACHBB7_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208688.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            17766..18371
                     /locus_tag="ACHBB7_000714"
     CDS             17766..18371
                     /locus_tag="ACHBB7_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9208689.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            18391..18753
                     /locus_tag="ACHBB7_000715"
                     /pseudo
     CDS             18391..18753
                     /locus_tag="ACHBB7_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18756..18938
                     /locus_tag="ACHBB7_000716"
     CDS             18756..18938
                     /locus_tag="ACHBB7_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208690.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            18956..22375
                     /locus_tag="ACHBB7_000717"
     CDS             18956..22375
                     /locus_tag="ACHBB7_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM9208691.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            22426..23163
                     /locus_tag="ACHBB7_000718"
     CDS             22426..23163
                     /locus_tag="ACHBB7_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9208692.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            23173..25464
                     /locus_tag="ACHBB7_000719"
     CDS             23173..25464
                     /locus_tag="ACHBB7_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM9208693.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            25488..27569
                     /locus_tag="ACHBB7_000720"
     CDS             25488..27569
                     /locus_tag="ACHBB7_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208694.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSITSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SDYTFAITYTI"
     gene            27586..27735
                     /locus_tag="ACHBB7_000721"
     CDS             27586..27735
                     /locus_tag="ACHBB7_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208695.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            27732..28178
                     /locus_tag="ACHBB7_000722"
     CDS             27732..28178
                     /locus_tag="ACHBB7_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208696.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            28180..28317
                     /locus_tag="ACHBB7_000723"
     CDS             28180..28317
                     /locus_tag="ACHBB7_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM9208697.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            28355..>28626
                     /locus_tag="ACHBB7_000724"
     CDS             28355..>28626
                     /locus_tag="ACHBB7_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208698.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT        10324 a         4276 c         6119 g         7907 t
ORIGIN      
        1 agtaatctat gtgggcaaat tctgttaata tgcttttttg atttaacaca atcaaactac
       61 tgttggacaa aaatgacagt aaggaaagag ttgctccgat tactaaataa aaaatagttg
      121 ttaacaatat tatattatca atttttctgc tcatgttttc accccaccag aattatatca
      181 aaaaggagag aagaaataat gcaagaatta gtaattttga aaaataaaga agctgtgact
      241 acgagcttac aagttgcaga aagcttcgat aaaaagcaca gacatgtttt gacggctatt
      301 gatgaactaa aagagggggt tgccgaaaat tgggcagacc tattttggga agatacttat
      361 gttcatcctc aaaataaaca atcataccga attatttata tgaatagaga cggcttctct
      421 ctattagcaa tgggcttcac tggtaagaaa gctttaagtt ttaaacttca atatattgag
      481 gccttcaaca agatggaaaa agaattaaaa gaccaattgc catcaattcc agcaactaaa
      541 cgagaaatgg cattacttgc tctagctgca aacgaagaaa cgaatgaaag agtagacgct
      601 atcgagtctg atttaaacga tttgaagaac aatcaacttc tagctgaacc agattaccga
      661 acaatttcta atatggttcg caacaaaatt agagttattt gtaatcagca gcatttaaat
      721 agcaaagcaa aagcagaatt gtttaaagac ttgaatggcg gaattaagcg aatcactgga
      781 gcagtagcta gaaatcgtat caaggcaaaa cagttcgatg atgttatcga ttttattaac
      841 aattggatgc cttctacagc gacgatgaca attattaagc aaatggagtt gttagaggat
      901 gaatagagct gaagcgctaa aaataggcgt aataattgct aatcgctggt ggagacacaa
      961 taaaccaaac atcctaagcc aacaacatat tgataagcaa aaagcatggc aacaaataaa
     1021 aaagtgactc agccgaccaa agcaatgagt cacaaagaaa atacatctaa ggagatgtta
     1081 ccacatgaaa aaagaacttt ccactctaga tcaatatttg actgatccta gttggggcaa
     1141 atcgaatatc aaggaaacaa gcaatcgaaa aatcagacgt aatcttttga cggatgaaga
     1201 actagcatgt gatcaagacg atttgggaaa ttttgtgagt atttgggatc atgtctatct
     1261 tatccattta tcgaggaagt ccaaaaaaac tgaatatatc tatgtcatcg aagatggctt
     1321 gattgatgcg ctagaagagt atgacagaga taacttgatt gatatctctt attacggatc
     1381 aggtaagaaa tacattgctg aaatggaggc agaatttgat gagtgaaaag gaacaacctt
     1441 tgaaaaatag aagtgataat acgcttttta ataccttata caaaataaat gtgaaagatg
     1501 ttactgaaaa acgaaataac ctcacttatc tttcgtgggc atgggcttgg gcagaagtta
     1561 gcaaagtgtg tgaggcagta gattatgaaa tctatcatga tccagaaaca tatctgccgt
     1621 atgtctttga taagaaaaca ggctatatgg tttttaccag tatcacagtc aacggagtaa
     1681 agcgtgacat gtggttacca gtcatggatg gtgcaaacaa ggcaatgaaa gatgagccat
     1741 atacctacga agtcaatgat tatcagtgga ataacgaaac gaagaaaaaa gagattgttg
     1801 gaaaaatcga aaagcgagtt gaagcagcaa ctatgtttga tatcaacaaa acgatcatgc
     1861 gctgtcttgt aaaaaatcta gcaatgtttg ggctagggct atatatattt gctggcgaag
     1921 atatgccaga agatgtctcg atgcttgaac cagctactca aagaagcaaa aagctattct
     1981 tggatgcttt acaactggtt gctaacaagt acgaaaaatc aattgatgaa gcaattgttg
     2041 cattgactga tgcggcttct ataaccgctg atgacagtaa atggaccaag agagacttgg
     2101 gcattctaaa acgaggcgtt aactggattg aagatcagta cagagaagaa acaaaagaga
     2161 agtgatatga gtgtttaaac cattgatcga ttcatattca gcggttctga aaaagttcaa
     2221 aggaaaagac ataggtgcaa cgatcaatga agaagtgaac attgatcgac taaagacgat
     2281 gtatgacgga tatgatggcg atcggattat tgaagttcga tttattgatc cacgtcgatt
     2341 tactgtgcag caacgaaact tcatctatgc gctgataggc gatattttca tcgatacagg
     2401 catgccaacg gacttctgga aggaattctt ttacttccgt tttgaaggcg tcacagggcg
     2461 caaaataagc ctcaaagacg aatcgaatac aactgtgagt gatgctaatg tcttagcaaa
     2521 tatcatctta gatttcatct ttgaacatca tattcctttc aaagaaggct atgagatttt
     2581 accagcgaat caagaatatt acttctacaa atgcatcaca aaaagagtct gctgcatctg
     2641 tggcaaaaca ggagctgaca tcgatcactt tgacaaagcg ctaggaagac gaaagcgcaa
     2701 agaagttgat cattcagagt acacatttgc agcactctgc aggattcatc acacggagaa
     2761 acacaatata ggtgtgatca atttcaaaaa taaatatcaa atcaaaggga tcaagttaaa
     2821 ccaggaaaca attaagaaac ttagaatagg agggtaaaaa atattgtctg acaacaaacg
     2881 ctactactat ttaaaactaa aagagaattt tttcgatagt gacgagatgg ttctcttaga
     2941 aagtatgcca gatggctata tttattctaa tattcttctc aaactttatt taagaagtct
     3001 aaaacacgaa ggtaagttga tgtttaatga caggattcca tttaactcta caatgctcgc
     3061 aactattaca agacattctg taggagtcgt agaaaaagcg gtacaaattt tccgtgattt
     3121 gcagcttatt gacgtattag ataacggagc aatttatatg tctgatatac aaagtttcat
     3181 tggaaaatcc tcaactgaag cagatagaaa aagagaatac agaaagaaaa tagaagaggc
     3241 aaaacggaat ttaataactg gaggacaagt gtcggacaaa tgtccagaca aaactacacc
     3301 agagttagag atagagatag agaaagatat agatatagat aaagaagaaa agaaaggtaa
     3361 gtattctgac gaacacttac gccttgctaa aaaattgcaa agtaatttaa ctgaagattt
     3421 tccaaaagaa atgaacaaag tagatatcga aaaatgggca gacacaatca ggttgatgga
     3481 agaaagagat aaagcgtcta tagaagcgat tgagtatgtg atcaattggc tacctacaaa
     3541 tgaattttgg tttggaaata ttagaagtgc taagaaattg agagaaaaat ttgagaagct
     3601 caaattcgaa atcaaagcag acaagaataa tcataaaaag caaagtcaaa aactacagta
     3661 cagcaatcct agtgaatatg acgacttgcc aatttaaaaa ggagatgcat cacatggaaa
     3721 gcctagcaaa tgctatggag aaactaataa gaagagtatt agtgcaaagc gggaaatgtc
     3781 cagaatgtag cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt
     3841 gtaaaccaac atgcatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga
     3901 ccgaacggat atataacgac agcttaaaag cacgagcgtt gagttttttt caaaatggtt
     3961 cggtattaac agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc
     4021 aagaaacgaa aattgcttta gaaagagcta aaagctttgt aaatgatgtc ttactgaacc
     4081 atcctgcaca tttcattcta tcagggaaat caggaagcgg aaaaagccac ttgtcaatgg
     4141 cgacagcttg ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct
     4201 atcaagagtt attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa
     4261 ttgaaggatc gcttatagcc gatattaaaa caactgattt ggtggttttt gacgatattg
     4321 gagctgaatt aggtagtggg gtatcaaata gtaggcagtt tacaaacaac acgttaaaca
     4381 cgctcttaga agcaagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg
     4441 aactaagaga agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt
     4501 atgcgctgaa attccagcaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg
     4561 catgaataaa taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc
     4621 agaagcagag tattacgatc tagccttgtg gcaagctgaa gcgaatggct ggaaagtaaa
     4681 acttcaggaa agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa
     4741 gatcgagtat attcccgact tcacatttta taaaaacggc aaacttgtca aagtcgtaga
     4801 tgttaaagga atgcagacaa aagactttaa gatcaaggca aagctgtttt gtcatcaata
     4861 tcaagtgccg ttgatattag ctaaaaaata tcggaatacg ttcaaggaag agcgttttta
     4921 aagaggtggt ctatcatgac aacagaagaa gtgattcaaa tgcgaattcg aagcattcaa
     4981 cgtgagattg acgatctgga acgaacaaag gcagtgatgg tcaatgaaac ggcgagaaag
     5041 gcaatcgatt tgcacataga gaacttaaga agggaaattc gtagattgga ggaatgagcg
     5101 tggataagaa agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag
     5161 aagacaaaga aatagttgca gaagtccaaa agctcggtaa atcaatgtgg actgaaaaaa
     5221 ccagacggag aatgccgaga aaaattgcaa tctggcacgg tgaccgaatt ctagtaacag
     5281 gtaccgctga acagttatct gaaattactg gtctgagcaa aaacatcatc tgggatagag
     5341 cgaagaatat ggatattgat tctaaaggaa gacagtttag gtatgtggag gagaaataat
     5401 gaatctcatt acacaataca gtgatatcat cctcaagaaa atcatgatga agattcagaa
     5461 agacaaaaaa tcaaaagaac gagcggaatt agttaagttg gaaatggctg aaacaggggc
     5521 aggagtgcga agtagcaggc attggaaggc agcagcaaac attgaatttt attacaacga
     5581 aattcaaaaa gggttcgatc aaatgcgtga gctggatcgg caaacaaatt ggagcaagaa
     5641 acttcatcaa gatcgtttca aatttgtaga gaaatataaa gagatattag acgaatacat
     5701 ggagggcagc aaatgatacc gagatttcga tcgtggtaca caccatttaa aggtaaaaca
     5761 attggacaag aaatgaaata tgggcaagca ggaaggttga tcactcatgc tgaaatggct
     5821 ccagataaat atgtgcttat gcaatccaca ggactgaaag ataagaatgg cgtagaaata
     5881 tttgaagggg atgtagttct ggttaatgta agtaatggtt ttgatcattt agttaatgaa
     5941 aaaacggttg tacaagaatc cgagtttcat tccggattag tttgtaaatc attaattagt
     6001 ggaatggaat atagaatatt cagccaccaa aagatgggat acgaatatga agtcaccgga
     6061 aatatacacg agaatagcga gttattggag gtagagtgat tatcttagct actgactaca
     6121 tcgattcttt aaaagatgaa aatgggaatg tcccagcatt gctaaattgt ggaattaaaa
     6181 gtaagaatag aatcattttg ttacggatat cggctgattt agcaaaatat ctttatctga
     6241 gcaagacagc agtatattac actgcggcta cacgttatca gtacaaggga aaaacaatca
     6301 gtcaggatta ttatgatcgt ttaatcagtc ctgacataca tggaaaatca aaatcagcga
     6361 tcaagttatt tggcgcaata gagatatatg ccgatgattt tccaaattta tggttgaagg
     6421 aggaacagta atgaaagact ggttagaaga agcaagcgcc ttagttggct gtttcataaa
     6481 ctgtgcgttt atatctgtaa ttattgcact ggtgggattt gtatcattga aagtgattat
     6541 attgctttgg cagttaattt tttaggagga acagcgatga ataaacagga agcaatcaaa
     6601 aagttagaaa gtattaaagt gataggaaat gatgcactag ctgcttgcta taacgagagt
     6661 ataaatgcag gtattacgtt aatgaaaaaa atagacgaac cgaagaagct agttattcca
     6721 caactcgtgg ccggttggct tgagaaatct acggaccctt ttacgaaagc tgaaaaaata
     6781 gcgtatttaa tcaaatctaa agatggtgat tcatattatt tctgtgattg gtttgtacga
     6841 gatggcatag tgacgcaaga acaaggagaa gaattactcg cttgggcaac gagacaatca
     6901 tatgaaacac tattgagctt gtacaacggc tacgaggtcg agaaagagcc gttgtacagt
     6961 gtaataattg ctggcgagta ccttgtcaag gagatcagtt gcagtaatga agttaaacta
     7021 gtatcaacag cttacctgcc acaatacctt gcacaccact accaactgac cgaaaaacag
     7081 atcaaagcaa ttgatgaaag atactggcca tttgctgtgc cagtggaaga ggtggcggaa
     7141 ggatgagtag acagataatg ctccatatac cagacggaac aaaggcaatg acgattacca
     7201 ttgttgctgc acaaaaagac ggtgggttgg cgttgaaaac aaaaagcatt gataccaaac
     7261 agattttaga aggcagagat gtcgagatag agatcgatga ggaggaagcg gaatgaatgt
     7321 tcaagtttat ttaaaaagag atttggccag gtttcctcga agctgggata caaattccca
     7381 cccatccgag gcagaagaag agggctatct aatgtctgct aacatgatag aaattacaag
     7441 agatgatgcg gatgagtttg ataaaaaaag atcatggtac tcggttccga atccaatgta
     7501 tacggctgta atagaagatt atcatatatc cgacagattt attctgattg atctagaaag
     7561 gcctaagaaa cgaattaaac agtatagacg ttgtggattt tcaacaaaga aatggtgata
     7621 acaaaacaga ggtgaagaaa aatgaacaac agacaccgca gagtagcaaa actaagaaat
     7681 caagaactga atgtagcgaa agtaaagttc gaacgggagt atggtgtttc tgcagaagaa
     7741 acatataaag tggcaagtca gtgtgttgct gatgcaagtg atgctattcg taagtttgga
     7801 atttcgatac tagatattaa atgggaggat acagaatgaa gatttatgta gtaaagtttg
     7861 gaaatcaatt ttacagaagc gatgaacgtt ctataggggc taacacatta tccattgtag
     7921 acatactcca aagcgcaaga tggtttgata atctcgaaga agctaaccaa gtttcacgac
     7981 gacttggtgg attaacgcaa gtatatgaac tggtcactgt cgatcatgag ggggtagagt
     8041 aatgaaacta aaagacggat tttacgctag tagtcatggt atcggcggtt tgatgctaga
     8101 tatgccgaca aagaacccta aaacacgtga gaaaccaaaa ttcaaagtcg gtgacatggt
     8161 tcgctgtgaa gcagaaggat ttatctatcc atttcgtgga tatgtagaaa aggtattgtc
     8221 gaactcagca atcattcgta ttgaaaacac gatggaatgt gacaagtggt tagcgaaaag
     8281 caaagagaat ttagctgtag cgagattagt tgacatggaa gtaatcgaga gcaaataaaa
     8341 aagccggatc gctccgacta acataataaa acagacaagt ttattatatc acataaagga
     8401 gcggtttgac ttgatgcaat tgttacgaga ggtagatttc aaacagacaa gatgtaatgc
     8461 gagagatgtg ctgaagaact ttcggcgttt ggagcggatg gcaggtcgct ctttgataga
     8521 tattaagtct ccaattatta cggatatgcc gaaggcgccg aagcacggca ataaggcaga
     8581 ggacgcgatc attcagatga tggatataga agcagagaga gatgcgattt tagcggcttt
     8641 gatggctctt agtctgatta gccgtcagat actctactac agcttttgtg tgccagatag
     8701 cttctcaaac tacagaatta gccgtgaagt gggttattca gaaagaagta tacaacggat
     8761 gaagtcggaa gctctaatag agtttgcaga agcatataaa cacggaagaa taattgctta
     8821 taaataattt ggcggttttt tggcggaatg atggcggttt ttagctattt accagtgata
     8881 ttatggtagt gtcgaaagat tagtgatagg tctgagacaa aataataata aaaggaacat
     8941 cgttttatta ttgtttcaca attaagcttc gatagacagc aacggaaata ttaagaataa
     9001 ggatgtgaat ttcaactcct tctaaattgt tcttattatc tatcatccgt tgctgtctat
     9061 tgttttttta attattcaca cgataactaa aggtgagtga agagaaatga ttccattaat
     9121 aatttcaatt tttgcgctct gtcttaatgt ctatatgatt ggatttaaaa atgggcaaaa
     9181 taaaaaatag tagcagccaa gaataatttt atagtgtcac tgtggcggaa agggtagacg
     9241 cttaaaaata aggtcaatac gtcgagggat agccttaacg ttttatgatt tgaccatgca
     9301 atgttcgatt cattgccagt gactttggca accgagggtt ggaaatgggc gctcaaagta
     9361 cacgagcaag gcgaggtcga tagtaatcga tggaatcggt gtaggttgct tgatagagct
     9421 ataactgcat ctcgtttatg aggtgtagtt tttacagatt agatcactct ttgagtggtc
     9481 tttttatttt acacaaagga ggtaacaaga atgtatagac cacaatactt agaacagaag
     9541 tatgaagtaa tcactgtgca aaacggtaac ggtgagatag tacgaaagta tagaagacca
     9601 ataaagagcg atacatacaa acgaaaggaa agcaatgaag ttattccatt gtatggcaaa
     9661 agaatagcta agcattaaat aagattgcga aaggagacgg aatatgaccg aggaattcta
     9721 tagatggcta ttacagttga taagagaaga tcgtttagtt aagttctatc agtctcctaa
     9781 atggcgcagg cttagagaga aagcgatgaa acgagatcac tatgaatgcc aagagtgtag
     9841 aagactaggt aagtatcata gagtagaaaa cgttcatcat ataaaggaag tcaaggatag
     9901 acctgactta gctttagatt tagataatct tatttgttta tgtgttgaac atcataatga
     9961 agttcatggc agatatctta cagcattaga taaacaagag aagaagatag aaagctttgc
    10021 taacttcgat gcaagtgaaa ggtggtaagt gcatgatcat caatgataat ggcagagagt
    10081 atgatacaga aaagattgaa gagtattcat cttatactca gggattaatt aaacgtttga
    10141 tatacgttcg ctatgtagat attagggatc tgttatcaga taactgttgt agtaaataca
    10201 aagtgaatca agtaagagaa gcgttgaata aagataataa cgtcgaaaga ataaaaaatg
    10261 tttttggata tagtattgaa gagattaatt attacattga cttcgctgaa gctttcattc
    10321 cgatggtgag ataacccccc cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg
    10381 agggggctgt caggaaaaga gattttttcg aactttatca tgaaaggagg gctaaaatgt
    10441 ttaaaaacga attgtctcaa aatcgctaca gagaaaaatt acgccgctct ttaataagcc
    10501 aattggaaag tcagaaaaca aatattgagc cattcttaga taatgttgat cgttatatca
    10561 gtttatggga aacggcgata tcactggaag aagatatatc cgagaacggc attagactgg
    10621 agaatggtaa aaagaatgaa tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat
    10681 tgatgttgga taaacttgcc attactcctg aattggtagg tgaagcaaat gaatcaattc
    10741 ctgagttata agcatattga aaattggttc aaagctatag aagaaggcac tatcaaggta
    10801 tgcaaagagc aattattgct aaaaaattat ctagaagaaa gagtctttac tagagaagat
    10861 atttacttcg ataagcagat ggtagaggat tcaatcaata taccagcaca atactttcca
    10921 ttcgaattaa ttccgtggga aaaatttcta caatgtttta tttatggtgt tcgatggaaa
    10981 aaagataaaa cactagtgtt caatagatat ctttcattaa tgggacgtgg taatggtaaa
    11041 actggttttg cttcttggaa caacttcttt ctactaaccg ctaaacacgg tattaaaaat
    11101 tatgatattg atatctatgc caataatgaa agccaagcaa agactagttt tgatgatgta
    11161 tttaaagtaa ttaaagatca tcctgattta gataaaaaag tatttaaagc tacgaaggaa
    11221 gttattcaaa atatcgctac aaacagcaaa cttcgttata acacggcaaa tgctagaaca
    11281 aaagatggga agcgaccagg tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat
    11341 tcaatgataa atgtggctac ttctggtggt ggtaaaattc gagattatag agaattttat
    11401 gatacaacta atggtcatgt tcgtggtggt ccgcttgatg acattataga agaatcaaaa
    11461 atgattcttt ctggagaact tggaattgac aaggatggag cagaattttc tagtttgttt
    11521 ccatttattt gtcgcttgga taacgataat gaagttgatg atcccgacat gtgggaaaaa
    11581 gcttgtccaa ctattaatta caatgcagat ttaaaacgga aaatgtttca agaatactct
    11641 caaatgcaac gtaatgctgg tttaagactt acgttcatga ccaaacgaat gaacagacct
    11701 atggaagata cacgatttgc tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa
    11761 gaatttcctg aaaaaatgga tgaagtgata ggaacagtcg attttgctga tagacgagat
    11821 tttgccagcg ttgggttgct aggaaaatac gataaagatg tttattttac acaacatact
    11881 tttatccacg aatcagccct tcgattacaa aacatcaaac gagaggttat agatatttct
    11941 atagatcaag gaaaatcaca gatcgttcat ggaaaaaata tagaagctga ttatattgta
    12001 ggttggtttc ttgaaatgag taataaatat tatattaaaa aaatcgctat ggatatgtac
    12061 cgtgcaaaaa tattgaagcc cgctttagaa gaagcaggtt ttactgtgga aattgttcga
    12121 agcggatctg ttacacatgg tatgttaaaa gatctggttg atgacctttt tattaatcaa
    12181 cgtttatttt ttggtgacga tgcgattatg cgttggtatt gcatgaatgt atatgaagag
    12241 catatttcta atggaaatat acgctatgaa aaaatagaac ctgaaactag aaaaacggat
    12301 ggcttttttt cattccttca tggtttgaat tttttagatg atatttatga ttctgctcct
    12361 gtaacagtca caaatagctc agtagaaaat acaggaactg gatttactcc tctagtattc
    12421 taacttgaaa ggaggtgaga aagtggggat ttttcaaaag gcggtaggat acttcacaaa
    12481 aaaagcaacg gttactttag aagaatactt ttgtaaattg caagttgatt ttgtgtatcg
    12541 aaaatttgcg attgaaactt gtattgattt gattgcaaat gcgatgagta aagcggaatt
    12601 caagtcatat gaagatggaa aaaataaaaa gaatgatctt tactataggc tgaatgtagc
    12661 tcctaataag aaaaataatg caacagaatt tagaaaaaaa ctgatcagga gattaatatt
    12721 ctacaatgaa gtattgatcg tttctccgtc taataattct agcgaaatat ttattgcgga
    12781 tagttgggat gtcacagaat atgcattgaa agatgatgtg ttttctcaag tgcaaattaa
    12841 caacatagtc cttgatagag aatttctaga aagtgatgtt atctatataa aatacgcaga
    12901 tcaacaaatt aggcaactag tcgatgcgta ttatcaagcg tatgggaaac tcatttctag
    12961 tgccatgaat gtttacaagc gctctaacgc tcgtagatac gtactgaaag ggaatttatt
    13021 ccgaccgcaa gacaatacaa cacaagatca aatcaataaa atgatgacat cacaatttaa
    13081 ggcttttatg gaagctgata atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt
    13141 agaagatttc agcggaaact tccaaagcaa ttcaagagat ataaaaaact taatagacga
    13201 catctttgag atgacagcag cagcgtttca cgttccgaaa aacctactaa agggagacat
    13261 gagtgggtta tcggatcaag tggacgcttt tttaatgttc gaaatcatac cgattgctga
    13321 acttattcag gatgcgttta acgctagtct ctatgaagta gaagaatact tgtcagggaa
    13381 ttttgtacgt gtggatacaa ctatgatcaa gattactagc ttcaaagatt tggttgacgc
    13441 tattgatgta ggcattagaa atggagtatt tacaatcaac gaaggaagag aacgcgttgg
    13501 aaatgatcgc tctgataagg cgatggcaga tgaaatattt ataactaaaa acaatcaaca
    13561 agtatcgaaa ggaggtgagg cgaatgacga caatgaaaac atttctagca gtaaagaatg
    13621 aaggcgcagt accgcaaatt tttattcagg gatttattgg ttctagttgg ttctttgaag
    13681 ggaatactga caagggaatc aaaaatattt tggatagtct aggtgatcaa gaagaaattg
    13741 aagtagtaat taattcaaac ggtggagacg tatttcaagg gattgctatt gggaacttac
    13801 ttaagtcaaa taaagcaaaa gttaacgttg tgattaacgg cttagccgct agtgctgctt
    13861 caattatcgc aatggctggc gatactataa aaatctacaa caatgcacaa ttgatgattc
    13921 accgcgcttc cacatacgga gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac
    13981 tggaatcaat tgataaatcg gtaaaggctt catataaaac acgattcaat ggcacagatg
    14041 aagcattgca agaacttctt gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt
    14101 atggattggt cgatgaaatt atcgatgcag aaaatagctc aggtactgaa gctaaaaaag
    14161 aacaaagcgt tgaagaaatt ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg
    14221 catttacagc agcattaaat aaaacatttg gacaaggaga tgtaaaataa tgacagttaa
    14281 aaatttaaaa ggtgtaacag ctgcaagcga ccaattgatg aaagctttta aagatggtaa
    14341 cgaagaatct tttagcgcag ctatggtaag cttatctaag gaaattcagg ataaaatttt
    14401 agaagaagca acagcaaaaa atcaagatca attagtatta atgaaccgtg gtcagcgtgt
    14461 attaactaca caagaaacaa aattctataa cgaagtagtg aaaaacgaag gttttgcagg
    14521 ggtcgaagaa ttagtgccag ctactgtatt tgaacgtgta tttgaagatt tagaacaatc
    14581 tcatccacta ttgcaaaaaa ttacttttgt taacacaact ggtgtaacag aatggattgt
    14641 gtcacgtgga gtcaatccag catggtgggg taaactttgc gaagctgtta aaaaagtttt
    14701 agataatggc tttgatgtaa ttaacatgaa gcagttcaag ctatcaggtt atattcctgt
    14761 atgtaaggca atgcttgatt taggtccagt atggttagat cgttatgtcc gtactgtttt
    14821 agtagaatca ttgagaattg cattagaaca agcaattgtt gatggtactg gtaaagatat
    14881 gccagtcgga atgatgcgtg acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa
    14941 agcagaacct attacagctt tagatgctgt aactatgggc ggtttgatgg cgcgactatc
    15001 aaaattcaat atcgaaggtg tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt
    15061 ggtcctaatt gtgaatccaa ctgattactg gtctaaagta ttcccagcta agactgtact
    15121 aactgctaat ggagaatacg tacaagtatt gccagtacca gtttcagatt tgcagtcaac
    15181 ggctgtgcca gaaggaaaag cagttattgg ggtagcctca gattacttca tgggtgtagg
    15241 atctacacta aaaattgaag cttcagatga ataccatttt gttgaagacg aacgcattta
    15301 tctagctaaa caatatgcaa acggtcaacc taaacgtaac gatagtttca ttgtattaga
    15361 tattagcgct ttgggaacta ctactacaac tacaaaacca acaaccacaa caactacaac
    15421 acaagcgtag gtgatcagaa tgaagtatat tctttgtcag ccggcaatca atcggtttaa
    15481 atgggagctt gaagtttgtt taactaatct gaagaaacta ggaatcaaag atatcgtatt
    15541 gcttttcagc agacacgatg atcagattcc tatttttttt gagaaggaat atggtgttga
    15601 agttcatgtg tacgatgatc tgcgggacga caaagagtat attccttcga ttaaaccata
    15661 tttatggtgg aaatatttag aagaagatca ttcgcgtgag gacgaccgat atttctatat
    15721 cgattcggat gtcattttca ataaaagaat taatttgcgc aaattgcctt ctaaagatga
    15781 tgtttggtat tgtagcgact gctgtagtta tctaagtctt gattatatta gaagctgtga
    15841 aaacggagaa aatattctaa aagatatggc aaacattgta aatgttacag tagaatcttt
    15901 ggaaactata aacactaatt caggaggcgc acagtgggtt attaaccgcc ctaaagcgaa
    15961 ttattggaaa aaggtttatc tggattctaa tcggctatat cgctacctta gagggcaaaa
    16021 aacaaatata aaaatctgga cagccgagat gtgggcacag ctttggaaca tgatgtattt
    16081 caatattggt cctaaagttc acgaggaatt agacttttgt tttgctactg atccaataga
    16141 aaaagttaaa gaagtaaaaa tcttacacaa tgctggagta acaacaaacg atgaagattt
    16201 atttttcaaa gggagatacg tgacttctac gccttttgat gaagatttat catttgtaaa
    16261 caagaaaaaa tgctcttacg catatgttaa agcaattaag gcggtggtta gatgacgcct
    16321 gaacaagtga ctgaagaatt gctaatagct gtgaaggata atatttacgt tacctggaac
    16381 gaagaagatg agtcaattaa aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa
    16441 gtgagtacaa cactttcttt ttctcctgaa agcttagaat acggattgct aatcgaaaga
    16501 tgtagatacg actggaatcg tgctttagat gagtttgaac aaaatttcgc tagtgagtta
    16561 ttaggtttca ttcaacatta tgcgctacaa gaatatattg caggtgatgt gaatggcgaa
    16621 taatcgtaga ctcgaagaaa cattcaacga tggttggtta aagattttga cgcaaactac
    16681 caaaagaaat gaactaggaa aaaagattgg tgtagaagat acagaaatca cttctttaaa
    16741 atttagaaat ctttccatga gagatagtga tataacagct atggatgcga tgggatcgaa
    16801 attaactaag aaagtaaaga ctccatttca tccaatcgcc aagaaattta ataaagatca
    16861 atattttatc gtaatcgata gtatgcgtta caacgttatc tatgccgatt acgataattt
    16921 ttatatctat ttttatcttg aaagtgtggg tgaatatggt gattgataat tctaaagaaa
    16981 aagaacgttt aaataagcaa atttctgcta tcaaaacttc cttagaagag cattttggcc
    17041 tcaaactctt tcaagactcc gtaggcgagg atgagctacc tgatgatttt aattacttca
    17101 ttctcgaaac aggagaaata gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc
    17161 tatatctaac tttctattca gaaaatagag aagatttaac aggagattca ctagatatta
    17221 tttcattgat tcaaaatcgt tcgattcgtt ttcagagaat ggatcccaat catttaaaac
    17281 tagagaacca agatcgctat atcgatcaat tggtatttac gtttagacga ttattgaaga
    17341 gtgattgtca tggctaaaaa tagttgggag ctaaaaataa atggacatga tgaacttctt
    17401 gtgcggatgg aacgctattc aagcgagagc gaacgactga ttaacgaagc attgaaatca
    17461 aaaggttcgg ctattgcagt ggataggatt acagaaaaaa ttcctgtttc tgaagcagat
    17521 ttaagaagag ggcaccaaca cgcaaaaaat agtcgtccac ttaagactca atacattaat
    17581 ttgggtttca tcattagacc tacaagaaaa tttgagtatt taaaatatcc tgatttgggg
    17641 ataggtactt ctaaaagaaa tcagccggac gaatttatga gaagaggatt aggtcttgca
    17701 cttgatccaa ttacagaact tctgattcgt caattcgata aattaaataa ataggaggaa
    17761 caacaatggc taaaacaaca acagtaacaa cttttgacaa tataagtatt aaaagaattt
    17821 cttttaattt taagaatgca acaaatgcaa tttcaactga ttgtaatgga caattagatg
    17881 gcgaaacaga aatgcagaga attgtaaaaa aatgcggttc aacagaagta aaatcgaaat
    17941 ctaaaccaat caatatgacg gtaacaatta ctgcacatgt accgatggaa gtttatcgac
    18001 gcttcaatgg gttgaaacaa gatgaacgta ttaaatcggg aatttactct tatggtcctg
    18061 attccgtagg ggaagatttc tctcttgctg cagagatcgt ggatgatttc gaagaaaaaa
    18121 ataagctgat tggtatgtta gcatgcactt cgaatacagg attaacattc tctattgaaa
    18181 atggtgcgga tgaagtagct gcgttagaac tagaaacaaa agttatgcaa gatgaatttg
    18241 gtaaatttta tcatgaagca attgttgcag aacttgaaga agacttaaca gatcaatgga
    18301 tgacgaatct atctgctgat gtgattaaaa aaacttcagt tgtgacaact acggccactc
    18361 aatcacagta aaaaaaacgg aggtagcgaa atgaacgaag attactcaaa aattgaacta
    18421 aacgatggaa caattttgaa tttagaacct aaactgaata tcaagaaatt attgatgatc
    18481 aatagagatt ttaacacaga cgagtttgca aaaatgtcga tgggaaaagg ctctatggat
    18541 attacagtta ttcaaggtgc aaaagccgta tatgtagctt atcgtcaagc gaacatggtc
    18601 gattacattt cattcgatga atttatagat aaatgagatt ttgatatgga ggttgcagta
    18661 gctgtataca gtactatgat gttcaaacaa gcacgtgatg cctatcaaaa agaattcgaa
    18721 aaagcaaata aggaaaaaaa gcttcaaaag taaaaatgcc aaagctctta gttgaaacgt
    18781 gggtcgatgt ctattcgatg ttgaccgacg ttttttctat gccttcagat ttggttttaa
    18841 gcgatatctg tttagatgac attttgcaaa tggcttacaa caagagcgct tatgaaggat
    18901 ggaagaacta tgcaataaat caatcccaga aaaactaaag gaaaggaggt aaaaaatggc
    18961 taaaaagaga acggaagcag aagtaacttt catagctaac gatgacggat tgaaatctac
    19021 gttaaaagaa atcagtgctg aattaactaa aaatagagca gaattaaaac tagaacaagc
    19081 tcaattgcaa cagactggtt ctgaatcaga caagttagga agtaaattat cttctttaga
    19141 aaagcagtat gaattacaaa gtcaaaaagt tgaagtaact agccaacgtt tagccaatgc
    19201 gaaaaaatat tatggagaaa attccaccga agttcagaaa cttgaaagag aactgattaa
    19261 tcaacaaaca gcgcaacaac gtttgtcaaa cgaaattgat aaaacgagta atgcactagc
    19321 tcaagcaaaa ggcgaaatac agacgtacga gtctacaatg caacagttgg atagcgaaca
    19381 aaaaaatgtt caagctagtg cttctctgat tgaatccgaa tacaaaaaat ggcaagcaac
    19441 tgctggtcaa tcagcttctg aagccgagaa attagcgaaa gcccaagaat atgtttctca
    19501 acaatctgaa aatgcggaga aaacgataga tatcctaaga cgacagttag aagctacaca
    19561 gtctgaattt ggcgctacat ccacagaagc aatgcagatg gaagcgaagc ttaatgatgc
    19621 tgaacgtgaa tttgaagagt taggacaagc tgctaaaaat gtagatacaa ctaacttgga
    19681 cgatatcgga agcaaaatag atatgaataa tttaatggaa gcttctgacg ttttaagcga
    19741 cattggcgat aagcttacag aattagggaa acaagcagtg gactctgcta acagtgtagg
    19801 tagttcccag agtaagatac aagctaattt tggtttgtct aaacaagagg ctgaagaatt
    19861 aacgaatgta gccagagaca tttattataa aggttttgga gaatcgttag atcagtccac
    19921 agatgcattg attttggtaa agcgtaattt aggagattta aataatcaag atttacaaaa
    19981 tatcacggaa caagctatgg tcctagaaaa caccatgggc gctgatatgg atgaaacgtt
    20041 acgtggtgta aatggcttaa tggtcaattt cggcttgagt gctcaagatg caatggattt
    20101 aatggtttcg ggtactcaaa acggtttaga taaaacgcac gaattaggcg acaatatggc
    20161 agaatatagc caattatgga gtcaaatggg atattcagct gatgaaacgt tcggaatgct
    20221 tcaaaatggt ttagatgcgg gtgcttataa ccttgataaa gtcaatgact tagttaagga
    20281 aatgggaata tcgttaacag atggtcgatt tgagcaaaac atggatatgt ttagtgaaag
    20341 tactagaaaa gcttttgaag agtggaaaaa tggcggagga acacaaaaag acgttattaa
    20401 ttccatgatt caagatttta gcaatatgga tggtcaatac gaccaattaa ataaagcttc
    20461 gacaatttgg tctgcgcttg gcgaagataa tgcgatgaaa gttgtccaat ctttaactga
    20521 tgttaaccat acatttgatg atgttagtgg atctgcacaa aaaatgaatg aagattctac
    20581 tactccgtta caagaattaa acggaaaaat agctgaatta aaggattcat tagctcctat
    20641 aggcaacaca atcatagatg cactcgaacc agtaattgat tttctaggaa agatggctga
    20701 tgcgtttaat aatcttccac aaccagtaca ggattatgcc gtagcgattg gcggattgac
    20761 tgctgcattt actttattaa tgccaataat agttggcttc atggctctag gtggtcctac
    20821 tacattaata ataggagcag ttattactgc tattgctgga gttatagcaa ttataaaaaa
    20881 ctggggcgca attactgact ggtttaaggg aatatggagt aaattcactg attggttggg
    20941 tggtacttgg gaaagtataa aagaaggtgc ctcatcagtt tgggatggag ttaaagaaac
    21001 ctggtctgga tttgtagatt gggttcaaga tatttggcaa ggagtttctg attggtttgg
    21061 agagctatgg agcggattag ttgaaggagc ttccaacatc tggcaaggag tccaagaaac
    21121 ttggcaaaca ttcgttgatt gggtttcaaa tatttggaac ggagtcaaag aagtatggtc
    21181 gattatttgg gcagacattg taggaattgt tcaaatacca tggacattaa taacgtcatt
    21241 gattcaagct ggtattaata ttatcgtggg tatttttgat gtagctggac agttattagg
    21301 cgcagcttgg caagctgttt ggacacctat ttctgatttc cttaaaaata cttgggatac
    21361 tatgacacaa tggataagca tcgcttggaa cggaattgta actacattcc atactatatt
    21421 tgatccagta gtggcatggt ggaatggtat atggacatct attagtacta cggcttcaaa
    21481 tatttggaat tcaattagtg caacagcttc tagtatttgg aacagtatca agaatacaat
    21541 cactagcttg gtacaagcag ctgctacagt aattcaaaat atttggtcaa ctgtatctag
    21601 ttggttaggt ggaatttgga attcaatcag ctctacagca tcaaatatct ggaatagtgt
    21661 gactagtagt ataagcaatg ctataaacgc agctaaaagt gccattcaaa gtgtttggaa
    21721 tagtatatct tcgtggatca gcggaatttg gaacggtatc aaaaacactg ctttgaatct
    21781 ttggaatgga attacaagca ctattagctc taaagtaaac gatggaaaaa atgcaatttc
    21841 aagcggttgg tccaatctaa caggtattgt ttccgacata ttcaataatg ttaaaagtac
    21901 aattgctaac atttgggaag gtatcaaaaa gactgttagc gctccgattg attggattag
    21961 agataaaatc agtggcatct ttgataattt gaatatttcg ataccacata ttccgttacc
    22021 acattttaaa ttgagtgggg aattcaatcc attgaaggga aaaatcccaa cgttgggtgt
    22081 tgattggtat gcgaaaggta gtgtgtttaa ttctccgaat attatcggtg tcggtgaagc
    22141 aggacctgaa gcagttttac ctttgaaaag atctgttttg caagaaattg gtgatcgtat
    22201 cttgagtagc acatcagttt catctagggc acaaacgatt caacctgtga ataactacga
    22261 attcaatttc acaattgatg gtaacgcaga tgaggttact atgaagcaaa caactcaaca
    22321 aatcattgat agcattacaa aaattcaaaa tgataatgct tcggcatggc gttaaacagg
    22381 agagtatttc tcctgttttt ttagtattaa aaaggatgtg aaaaaatgac tgattgtata
    22441 cattctataa tcgatggatt tcctgattat ttgcataaat tggctttagc tgaaagacca
    22501 accatacctt ctccaaaaag acagagagtt gaaacttctg ttttaggtag gttaggtggc
    22561 ttagtacaag attactcgtt tgaagatatg tcgtttacat tgcactataa ctatttagag
    22621 gatgtggaag accatcaagc gttcaagcaa tcgttttata tcatgcgtca ttggttaaac
    22681 tatgcaaaga aattagaatt ctctgacgat cccaacgtct attacgttat ccagactatc
    22741 gatattgggg atgcagaaaa cgatattgtt gaatggggag agttcgatgt aaatattact
    22801 gcgaaaccat tcgcaagagt tcaagaagat gtacctataa ccgtagataa accacagtca
    22861 tttaacttgc tgaataatag tttagaagaa agttttccaa agattatcat cactccttca
    22921 gctacttcat gccagttcat cttaaatgat tatgtgttta gtttcgaagg cttagtagca
    22981 ggaactgatg tagtcattga tagtgatttg atgctttgct acgaagagca atcggacgga
    23041 gatattttag atcggtccaa caaaatgaag accatgcaat atccaacgtt gcaagtggat
    23101 attaatcatt ttaattgtac tggtttgagc aaaatacaaa tttatcgtaa cgggttaagg
    23161 taggtgaaat agatgatcga taatttaata actatttacg ataaaaatga cgcgaataat
    23221 ttagctgaac atttatatga tacgcaaggt ttaggcgctt tgtcagactg gttaacagct
    23281 actgttagca ataaactaaa cggagccgag atatttcagg gtacttattc aataaacgga
    23341 actaatgcag atttgattgt agaaggacgt attattcagt gttatgtaga tgaaaatcga
    23401 gcaaaacagc gtctacggat ttattatgca aaaacttctg taataggcaa tacgatagaa
    23461 gtaaaagctg aacctatttt caatgatata agaaaatcgg tgttgaataa atatgacagc
    23521 ggaacagaaa agatcactgc tactcaggca tggcaaaacg caaaagtttt agcgaaacca
    23581 gctatccctt cgcagttttc tttctcgtca ttagtagata cgcttgctaa tgtgaagata
    23641 gaaaaggcga attttttaga attctttggt ggaaaagagg gatctattct agatcgattt
    23701 catggtgagt ttctaaaaga taataacaca ttacgtcatg aaaaaagtct aggtacggat
    23761 cataaaatca aagcgattta tactaaaaac ttaactggtc ttgacttaga gattgatgct
    23821 caaagtgttt tagttggagt ttatccattc attagcagct cttcagaagg agaagacgag
    23881 atcactctac cagaagaagt tattttcacg gattacgtgg atgattatcc tgctggatat
    23941 gtttcttttg ttgattttaa agacaaagcg actgatgtag ccacattaag ggaagctgct
    24001 aaagactggt tgaaaacaaa catagataaa caaaaaccac aagtgagtgg ttcgattgaa
    24061 ttagtaccat tgaggcatca aagaggctat gaaaaatttg ttgatctaga aaaagtttcg
    24121 atgggtgacg gagtagatgt gtatcatcca cagttaaaag tgaatatgtc agcgagaatt
    24181 gtggaatata cgtttaatgt tctaaccaat tcatacgata aattagttgt aggaaacgtc
    24241 aaaacaaact tcttagaaaa cacagagaat aatgtcagca atttgattaa tgatgccatt
    24301 gatcaattga aaaatggtgg cgaaatcagt gatttaatca atgatattgt agatcatcaa
    24361 actgatatga ttactggtca aaatggtggt tacgttttat tagatcctaa agaagcccct
    24421 agtcgtattt tgattatgga cacaccagat aagaatactg cacggaacgt tttgcaaatc
    24481 aacaacgctg gtattggttt ctctaaaact ggcattaatg gaacgtatga caccgcatgg
    24541 acgttagatg gcggattcaa tgcctcgttt attacagctg gtgagatagt agggattact
    24601 attagaggta ctacattaat tagtgatggc actgattata gaacaagtat agctaatggc
    24661 aaaatgactt ggtactcaaa aaaagttaac aaagatatta tggagctaga agcacgtgat
    24721 tatgtaagtg ctgatgcagg tattgtatca tacaccatga aaactggtgg tggtttcatg
    24781 attagaaatc cacaggggaa tttggttttt agtacgtggg ataatggtaa taacaaaccg
    24841 tttttatctt ttggtgcgcc caatttcagg tatagcaatg ctagttatat aactgatggc
    24901 gatggtagtt ctttaagcat taatggtagt gcgggcagtt catgggagtt taaagtagct
    24961 ggtaggacta tgaaatttac tagtgatggt atgctaacgt tgccaggttg tttttttggt
    25021 tcatgggaag atggaaaact tgctaggttt gaacaatcaa cggtacaagt atataaaaat
    25081 tttactgtta gaggtactaa aaactcaacc gtaccaacag aacattatgg acaacgacta
    25141 ttaaacgctt atgaaactcc agaatattat ttcgctgatt atggggaagc cgttacaggt
    25201 gatgatggta aagttcgtgt tgatattgat cctatgtttg ccgagacagt aaatctaagt
    25261 cgatatatga cacatgtgac acctacagag ctagttttgt gtgctgttac tcatgaagat
    25321 attgaccatt tcatcattga aactagtaag ccaaacgtat tggttagatg gaatttagtg
    25381 gcacatcgac tagggtatga agatgttaga ttaaaagagg atacagcata tgatagcaca
    25441 gtgcttgacc aaaaacgttt ttaaaacgaa gacaaggagg tatataaatg gctagcagtt
    25501 tatataattt ggctttagat ttcagcaaag aattaaacta caccaaagct attatggctc
    25561 gtcaaggtga caaaggaatt acggtgactg ttaaaccgtt tttaaatggc ttgcagatgg
    25621 atacgagtgg cggaaccttt acattaaaag gaacaacacc atctaaccgt tacgtagata
    25681 gtgtcgctac tagtataact agcgaagaag tcacattttc tcttgatgga acatttatga
    25741 gtgaagcagg atactataaa cactgctacg tagaatatag aaaagataat caaattttaa
    25801 cgacgcaaga tatcattttt ttctcactag gagtgtctga catttcgcaa ggccaagccg
    25861 atgaatatgt ttcgcaatta gaagaattga ttcgaaagta taacgaaact tttgatgctt
    25921 ttctggctga aattaaaggt agaatggata gcttaaataa acagattact gatttaactg
    25981 gtcaagctaa aacactacaa gacaagttag atgctctgaa agaagaaatt tctaaattag
    26041 gtaacttaca tgtgatgtac tccaacagca tcgacttcgg gggctatgat tatagtggga
    26101 atccgaattt aatgtccaaa ctaaaatcga gcgattttaa cgttggttac cacgggtcac
    26161 taactaatgt taacggaatg ttacatttta tgtctgatgg tacaggggac attgatatgt
    26221 ttacgcgtat taatacacct cagcttgcta gtgggaaaac ctatactctg agtgcgaaag
    26281 ttcgatttga tgaaggaact acaggagcta ttgataaatt acgtttggtg tatcgtacat
    26341 caccaggaga aaagatatta ttggaagcaa atagtacaaa tattacaaca gatgatgtag
    26401 ggaaagaaat aacaatcaaa ggtacagcta acgttaatta tcaaatcaca aatttagatc
    26461 gattttatat tagtgttagt ttttctaaca gggataaaat aaatggtgga tttaaattgt
    26521 acgacatcaa aatcgaagaa ggctcaacag ccactccgta tcagccaaac ttactagatg
    26581 acccttactg ggtaggtaaa acatcgttgg gtaagaatat tgcaaataaa ggtacgacgt
    26641 tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa gagcttataa
    26701 taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa acctttgtag
    26761 catataatta ttggaacgtt aattttggag atctaaaacc agttgaggga ttgacagacg
    26821 tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa gaccttcgca
    26881 tttttcagtc gcctaaagaa acggccggcg catgccgaat tgactggctc aagatcgaaa
    26941 aaggcgacac acgaaccccg aatattgagc aatataaata ccgaggaatc ggcatgcgag
    27001 actcaaacaa tccaaaagat tatgtatggg atctagaacc tagctatgtt gagaataacc
    27061 tagctacaga tatcaaggtt gctgaaatca caggtaaagc aaacaattat accgatggga
    27121 aagtatcgga gattaattcg cagttgactg cttcaattaa tgaagtagac accacagcta
    27181 aggatgctca aacaaaagcg aatgctaatg cgactgctat agatgaatta gacaataaga
    27241 tcgatgaacg cattaatgat acagctacta ccacattaac agttacaaac gggaataccg
    27301 gatcagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg gctttaaacg
    27361 gaaaacgcag tggtggaaat gattcaacga tactaacaat tccagaaggc tatcggccac
    27421 caattagttt tgagcaactg gttggctcga tagaccgttc tactttgaac agtgctcagt
    27481 tatctattgg tgcagatgga gccattaaat ggcgaagaaa ctcaagttat ggatcggatt
    27541 atacctttgc aattacttac acgatttaga aaagcgtgaa tcgatatgaa ggcagcatat
    27601 agaccaattg aaccttacgg attcgagcaa atcattgtga atgatgaaga acatttaccg
    27661 gaagaatgca cagaagtcga accaccgatt ccaaattgga aaccgaaatt caattactgg
    27721 gagggaaata aatgaaaaac atttggaaat atggacgtac tggcggagag tacgcaggaa
    27781 aagtattgga cgacatgctt gtatccgttc cttacacaga tcagccaccg cttgaaggaa
    27841 ttcgtgctga tggcgaaccg ttaacaatcg ctgatcaaat gtttgatcct aaattgaatc
    27901 aatggattat tttagcgaac gcactagatc acaacgattt aaacaatctc aaagcaatgt
    27961 atgagtcgtt agaaaatgag aacggcgatt taaaacagat caatgccaaa ctcatgctaa
    28021 gcgatgtagc aattaaacag gaaaatactg cattgaaaga aaaagcggat agtttagcac
    28081 aaatcaattc aaaaatgatg cttgcttcgt tacaaaatag caaagacatt tcagaaatta
    28141 aagagcaact aaatccagct tcaaagggag gtgagtagta tgtttagttt tagcgatgtg
    28201 aaaatgatgt atgattgggg ctgtttcact aacgaacaag taatggtttt cgttccgttg
    28261 tgcattactg aagaaaaagc agataaaatc attagcaaag aagagagcgc atcttaattg
    28321 atgtgctttt tattttgatt caaggagttg tcacatgatt aatttagggg aatggggagc
    28381 gatagcagga tcaataaccg ctatcgtttc tttgatttta ttagtaataa aaccaattac
    28441 tgcatctttc tcgaagatta ctgagactct ttcaaaagta agtcacaatt tagatttgct
    28501 gactaaagat ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa
    28561 acacgatgaa agattagata cacatgcaga aaaattggta gaacacacgc aacaaattaa
    28621 aacttt
//
LOCUS       DAYYKN010000019        28119 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000019 DAYYKN010000000
VERSION     DAYYKN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.019"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..304)
                     /locus_tag="ACHBB7_000725"
     CDS             complement(<1..304)
                     /locus_tag="ACHBB7_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9208699.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKS"
     gene            complement(306..503)
                     /locus_tag="ACHBB7_000726"
     CDS             complement(306..503)
                     /locus_tag="ACHBB7_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM9208700.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(897..2624)
                     /locus_tag="ACHBB7_000727"
     CDS             complement(897..2624)
                     /locus_tag="ACHBB7_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM9208701.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2617..3810)
                     /locus_tag="ACHBB7_000728"
     CDS             complement(2617..3810)
                     /locus_tag="ACHBB7_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9208702.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3997..4641)
                     /locus_tag="ACHBB7_000729"
     CDS             complement(3997..4641)
                     /locus_tag="ACHBB7_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9208703.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4735..4869)
                     /locus_tag="ACHBB7_000730"
     CDS             complement(4735..4869)
                     /locus_tag="ACHBB7_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM9208704.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4871..7003)
                     /gene="feoB"
                     /locus_tag="ACHBB7_000731"
     CDS             complement(4871..7003)
                     /gene="feoB"
                     /locus_tag="ACHBB7_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM9208705.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7000..7473)
                     /locus_tag="ACHBB7_000732"
     CDS             complement(7000..7473)
                     /locus_tag="ACHBB7_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM9208706.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7874..8098
                     /gene="nrdH"
                     /locus_tag="ACHBB7_000733"
     CDS             7874..8098
                     /gene="nrdH"
                     /locus_tag="ACHBB7_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM9208707.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8257..10416
                     /gene="nrdE"
                     /locus_tag="ACHBB7_000734"
     CDS             8257..10416
                     /gene="nrdE"
                     /locus_tag="ACHBB7_000734"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM9208708.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10466..11431
                     /gene="nrdF"
                     /locus_tag="ACHBB7_000735"
     CDS             10466..11431
                     /gene="nrdF"
                     /locus_tag="ACHBB7_000735"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM9208709.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11520..12659)
                     /locus_tag="ACHBB7_000736"
     CDS             complement(11520..12659)
                     /locus_tag="ACHBB7_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9208710.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12968..13681)
                     /locus_tag="ACHBB7_000737"
     CDS             complement(12968..13681)
                     /locus_tag="ACHBB7_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM9208711.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13935..14636)
                     /gene="nagB"
                     /locus_tag="ACHBB7_000738"
     CDS             complement(13935..14636)
                     /gene="nagB"
                     /locus_tag="ACHBB7_000738"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM9208712.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14870..16402
                     /locus_tag="ACHBB7_000739"
     CDS             14870..16402
                     /locus_tag="ACHBB7_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9208713.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16593..17795)
                     /locus_tag="ACHBB7_000740"
     CDS             complement(16593..17795)
                     /locus_tag="ACHBB7_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9208714.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17877..18566)
                     /locus_tag="ACHBB7_000741"
     CDS             complement(17877..18566)
                     /locus_tag="ACHBB7_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9208715.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18672..19040)
                     /gene="rplL"
                     /locus_tag="ACHBB7_000742"
     CDS             complement(18672..19040)
                     /gene="rplL"
                     /locus_tag="ACHBB7_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM9208716.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19093..19596)
                     /gene="rplJ"
                     /locus_tag="ACHBB7_000743"
     CDS             complement(19093..19596)
                     /gene="rplJ"
                     /locus_tag="ACHBB7_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM9208717.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19800..19927)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20006..20695)
                     /gene="rplA"
                     /locus_tag="ACHBB7_000744"
     CDS             complement(20006..20695)
                     /gene="rplA"
                     /locus_tag="ACHBB7_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM9208718.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20799..21221)
                     /gene="rplK"
                     /locus_tag="ACHBB7_000745"
     CDS             complement(20799..21221)
                     /gene="rplK"
                     /locus_tag="ACHBB7_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM9208719.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21564..22232
                     /gene="sdaAB"
                     /locus_tag="ACHBB7_000746"
     CDS             21564..22232
                     /gene="sdaAB"
                     /locus_tag="ACHBB7_000746"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM9208720.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22245..23117
                     /gene="sdaAA"
                     /locus_tag="ACHBB7_000747"
     CDS             22245..23117
                     /gene="sdaAA"
                     /locus_tag="ACHBB7_000747"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM9208721.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23208..24308)
                     /locus_tag="ACHBB7_000748"
                     /pseudo
     CDS             complement(23208..24308)
                     /locus_tag="ACHBB7_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24400..25287)
                     /locus_tag="ACHBB7_000749"
     CDS             complement(24400..25287)
                     /locus_tag="ACHBB7_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9208722.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25364..25909)
                     /gene="nusG"
                     /locus_tag="ACHBB7_000750"
     CDS             complement(25364..25909)
                     /gene="nusG"
                     /locus_tag="ACHBB7_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM9208723.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26010..26180)
                     /gene="secE"
                     /locus_tag="ACHBB7_000751"
     CDS             complement(26010..26180)
                     /gene="secE"
                     /locus_tag="ACHBB7_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM9208724.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26204..26356)
                     /gene="rpmG"
                     /locus_tag="ACHBB7_000752"
     CDS             complement(26204..26356)
                     /gene="rpmG"
                     /locus_tag="ACHBB7_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9208725.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26518..27162
                     /gene="cbpA"
                     /locus_tag="ACHBB7_000753"
     CDS             26518..27162
                     /gene="cbpA"
                     /locus_tag="ACHBB7_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM9208726.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27218..27721)
                     /locus_tag="ACHBB7_000754"
     CDS             complement(27218..27721)
                     /locus_tag="ACHBB7_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM9208727.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27734..27780)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         8511 a         5811 c         4969 g         8828 t
ORIGIN      
        1 gagatttatc tccataatct gctcgatata aaccatttat tccagcagtt cttccaattt
       61 cactcaaatt tcttattaat tcctcttgat attcagtgaa tcttatttta ttagtataag
      121 cagtaaaata tagataattc accaatctat ttattaaagg accgggatct aattcatatt
      181 tttgtttttc taattttaaa tatacctttt gtaaaacctc aagaatatca gaatatttag
      241 gatctttact tatagatata tcattatata attcatgaac aatttgctta gcattttttt
      301 tcattttagc acttccctgg aattgctcca cctaaaaaac cacctataga cattcctgca
      361 atacaagtag ttgccttggc ccaatcgacc gtacatttat ttttagtgca atacactcca
      421 tttccataat attttccact atgagtggta cccccataga taagttgtgt ctctttaata
      481 gacaaaattt ttaaatgttt cataatctaa tcacccctaa attaatgttt ttagactatt
      541 attctaacat tcattaaatt tcaaaaaaca attccttctt gaaacgtagc aaaataattc
      601 ctgaataaga atttaatcac ttttaagtat ataaactatg tatattcttt aatatttttt
      661 gagaatgcac ttcgtacata ataaacttta atcacattag tctttcatac ctacgatcga
      721 tctctttgac tcgcacccat aaattcataa tagtttgtcg gcgtctttgg acgtttcgaa
      781 gatgagctac gcattctctt tttttataga caaaaaagat agagacgaac atggagcttc
      841 caaatttgga tatttgatcc aattttggaa gctccattta tttcgtgtct gtctttttat
      901 tttttccagc tttcgactaa ttcttggttc tctttgatcc agtcttttgc tgcttgctgt
      961 ggatcttttc cattgttgat ttccaacata actttttcca tatctttttc tgaccaatgg
     1021 aaattgtcta atactttgta agcttctggc tggtcttctt tcaatccttt tcgaaccatc
     1081 gtatggatct gctcttcgct tcccattccg ttttcaggat cttctaagta tttcagatca
     1141 tatttggcaa acatccaatg aggtgtccat cctgtaacta caattggttc gtgttttttg
     1201 atcgcttgac ctagtgcaac ggtcattgct cctgaagaag atgtctctac tttccagtct
     1261 ttcaagttgt catatttttg gatcgtgttt tcagcagccg taactacacc agctcctggc
     1321 tcgattccta tgattttctt acctgcctga tcggttaact catcgatcga atttacatcc
     1381 atgtaggctg gcactgctaa accaactttt gctcctgtca aattggcacc taggtcttcg
     1441 acttgatctt tatattgtgc atattgtgaa ccatgagttt taggtagcca agctgaaacc
     1501 atggcatctg actcgccatt tgaaacagat ttccacatga ttgaattatc taatggcgtc
     1561 atcgttacat catagcccat ttgtttcaat acttcaccga ctacatttgt tgaagcaact
     1621 tctgtatccc attcaacata agataagttg atacgttttg tttctttggc agatgaaaaa
     1681 ctccctatac caatcgcacc taagatcact actactacag caccaatgat cagtccttga
     1741 cgttttttac ttttctgtgt tgttgctcct gcagcttttt tattgtttgg ctggttcaaa
     1801 tgctgtgtaa aacggtcaat gatgattgct aagataacta atgcaacccc gttgacaaat
     1861 ccattcccga cttgtgctct ttgcaatgct gataatacgc cgcgtcctaa gccgggtgca
     1921 ccgatcatag aggcaataac gaccattgaa agtgacagca ttgttgtttg attgatccct
     1981 gccatgatcg tacttttagc taaaggcaat tctagtttga atagtttttg acgacttgtg
     2041 ctcccgaatg aatcagaagc ttctactaat tctttaggaa cttgtcgaat ccctaaattc
     2101 gtaaaacgaa cggttggtgg cagagcaaag atgactgagg caaatacccc tggaaccatc
     2161 ccgataccga aaaatgcaac tgctggaatc aaataaacga atcctggcat tgtctgcata
     2221 aagtcaagga tcggtttgat aatactttgg gctttactgc tttttgccat caagatccct
     2281 aatggtaccc caatgatgat cgagatcaca ctagataaaa gaacaagtgt gacagtattc
     2341 atcaaatcat tccatagacc ttggttataa ataaaccata gaccgattaa tgtgaacaac
     2401 gtaagtcctg gacgtttctt agaaataaag aaagctgcta ccgtcaataa caagataaac
     2461 aaaggagctg gaatcactaa cagtaaattt gtaatccccg acatcactgc ctgtccaatc
     2521 gtttgtaaaa atgaaaatag gccagaaaaa gttgtcgtaa accattctgt gatattttct
     2581 acccagtttg ccactggtaa ttgatagttc aatagattat tcatgttcac tcacctcgct
     2641 tgtttctgct aatgcttcaa tcacactacc tcggatgacg acacctttta attttccatc
     2701 ctctacaact gcaagtggag tcggtgagtc ataaataata ttgaaaatat cacttaccac
     2761 catgtctttc ccaatcaccg tcacattttc atctaggaag tcgatcaatg gacgtttttg
     2821 cttcctagct tctaacgctt gatctgcagt gatgatccct ttcaggtgtc tctttctgtc
     2881 aacagccatc agcatgctga cttcttcatt acgcatcctt gtcaatgcaa cgtttggtcc
     2941 atcagattcg atattcgtcg tcaatgcagg taccatgatg ttttgagccg taagcacttt
     3001 ggaacgatcg acttcttcca caaattcacg tacataatca tttgctggat tggtcaatat
     3061 ttcttcacct gttccgatct gcatgatttc gccgtctttc atcaaggcga ttctgtcccc
     3121 aatccgtaaa gcttcattca aatcatgggt aatgaaaata atcgtttttt gaacattggc
     3181 ttgcaggtcc agtaattcgt cttgcatttc tcgccggatc agcggatcaa gagccgagaa
     3241 cgcttcgtcc atcagcaaga tctcaggatc atttgctaaa gctctggcta ggccaacccg
     3301 ttgttgcatc ccaccagata actgactagg atattggtct ttaaatgaaa gcaaactaga
     3361 attttctaat gctttttctg ctttttcttg tcgttcttct ttaggtacac cacggacttc
     3421 taacccgtat tctgtatttt ccaagatcgt tcgatgtgga aacagaccga agttttggaa
     3481 aaccatattg attttatgac gacggacttc tcgcaattct tctttgctta acttcgatac
     3541 atcttctcca tcaatataga tactgcccga agttggctca atcagacgat tcaatagacg
     3601 gatcaatgtg gatttcccac tcccggaaag ccccatgatc acgaagattt ctccttcttt
     3661 tacgtcaaaa ttcacgtcat acacaccaac cgttgcaccg gttttcttta aaatctccgt
     3721 tttcggctgg tgatcattca tcatatctaa agcggcttgt gtctttttac caaatatctt
     3781 tgttaaatgt tttacttcta cttttaccaa acaaattctc cttttttatg taaattcatt
     3841 tttctttctg aaaaaagtga ccacactatg ttaacatcat gtagtcactt tgtcaaaaat
     3901 gaaagaaatt tgcttgtcca aactgaaaaa aacttggcta aatctttgta aataaaaggt
     3961 ttagccaagt taatattttt ttaatatttc acacaatcat tctgcaagct gatgctccgg
     4021 gtaaaaaaag tttgtcatac gctgtagtgt atattcattt ccaataaaat gaacgatgtc
     4081 ccctgccgta aatttagcat aggggccagg tgaaacaagc agttcttcat tatgagaaat
     4141 tgcgataact gtggcgccag tcgcctgcca gatattcaac tcgctaattg aacggtccag
     4201 atgcattgct ttttctgata aagataattc aaacggaagt aaaggattca tcttatgtat
     4261 tcgcttcgtt tgattgacca gctgatccaa taattgagaa aaatgattca gctcatcttg
     4321 ttgcttctcc acacttgcca tgatttctgc ttttgtatct tggatggatt gtacatcttg
     4381 atattgcaat aggaaatctt ttgctttttc cttcgaagca acaaatgccc cacttccatg
     4441 ccgtacttcc atgattccaa gatctaccag cacattgata gccttgcggg tagtctcagg
     4501 agaagcattg tacgtactgg caagtgttga gcgggcatgg atcttctcac ccactttata
     4561 tcggttttca acgatacgtt cggctaaatc aacagcaatc tgctggtagc gcgggcggac
     4621 gatttttttc tttccagaca ttttctttcc tctttctttt tttactttcc ctcatcttac
     4681 tatgctctag caaaataagc aaaaaagggg agaatagcct cttgcaacga ctgatcatcg
     4741 cttgatctgt tcctttttta ctgggcaagc tgtttgacag tcatcacaat tcttaccgga
     4801 ttttaaacga gtatagacaa ttgcgcctgc acttccaaag attaacacac ttaataagaa
     4861 tgtcgccatc ctattcctct ccttctaatg acaatatagt ataaaactgg ttcttggatt
     4921 ttggctgacg gaacaaaaca taaacgagtc ctatcatcag taaaatagca agatacgtcc
     4981 ctaacgctac ctgtccaccc tccaataagt gtcccaactg ataaatcaca aaactaacta
     5041 aataagctaa accgcattga tagcctatgg cgatccaagt ccatttcttg gcattcattt
     5101 cccgatggat agccccaatt gccgcaaaac aaggcgcaca aagtaaattg aaggctaaca
     5161 atgaataagc tgctgcggga gtatatgtgt gttgtagcac ttgccagatt tctctgccat
     5221 tttcggatac ttccgcatga ccaaacaaaa taccaaatgt gccaataata ttttctttag
     5281 cgaccaatcc tgtaatcgct gcgactgttc cttgccagtt tccccaacca agcggctgaa
     5341 aaacaactgc caggattcgc ccgaaaaagg ctaagatact ttggtcttca gaaacaggtt
     5401 gagcataaaa attataagaa gagctgaacc agatcacgat actagaaaca aagataatcg
     5461 ttcctgcttt tttaacaaaa gacttgctgc ggtcataggt ttgtcttagg accgctgacc
     5521 attgaggaaa atgatatgcg ggcaattcca tgataaaagg agctgtatct ccagcaaaca
     5581 aacgtgtttt tttcaagctg attccagaga aaataattgc agtcatacca agaaaatagg
     5641 cagaaggagc tacccaagat tgatgaggaa aaaaggcccc tgctactaag gcaataatcg
     5701 gcaactttgc agaacaaggc atgaaggttg tgaccatgat cgtcaaacga cgatcttgct
     5761 cgttttcaat cgtgcgactg gccatgacgc ctggcactcc acatcctgta gcaatcagca
     5821 taggaatgaa ggattttcct gaaagaccga atttcctgaa aagtcgatcc atgacaaaag
     5881 cgatcctcgc catatatccg cagtcttcca gaaagctcaa gcataaaaac aatataatca
     5941 attgcggtag aaatcctaac actgctccca cgcctgcgat gatgccgttc aagatcagct
     6001 cctgcatcca tcccgctact tgccattcag ctaacaatct gccaacatgt tctggtaacc
     6061 atgtaccaaa caatgtgtca ttgatccaat ctgtccccat tgttcctact gtttgaatag
     6121 ccagataata gatcagccac atcacaaatg cgaagatcgg caaggctaac caacgattcg
     6181 ttgcaatctg atcgattttg tcactcatcg ataattggaa agaatcattt gacacaacac
     6241 agagtgtaca tagttgggta ataaattcat aacgttcatt gaccagaatc gtctcactat
     6301 cgtcgtccaa tagtttctct gttaactgga tcagttgctc gatctctttt tgctgagaaa
     6361 ctgctatctc gtaatcctct cttaccttct gatctcgttc aaaatatttc attgcatacc
     6421 agcgtttctt gccgctttgt tctcctagta catcttcaat ttcatgtaaa gctgcttcta
     6481 aacgtggatc atatgtacag ggaacaggaa tagaaggccg cttttttgca gactcctgaa
     6541 tcttttcgat tagtttttcg attccttgct ttttcaatac gctgattggt aaaactggta
     6601 caccaagacc ataggaaagc ttttctagat tgattttttt tccttctttt ttgatgacat
     6661 ccatcatatt cagaccaatg atcataggaa tgtctaaatc caataactgc gtagttaaat
     6721 aaagatttcg ttctaaattg gtagcatcaa taatgttgat gatcagttca ggctgttcct
     6781 gaaccaaata atctcttgca atcacttctt ctggtgtata aggagacaaa gaatagatgc
     6841 cgggtaggtc ctgaatatga atgttcggat tttttttcaa cgatccttct tttttttcta
     6901 ctgttacgcc cggccaattc cctacatatt gattcgaacc agtcaagaga ttgaacaagc
     6961 tagttttccc gctgtttgga tttccgacta acgcgatatt cattcttttt cctccctttg
     7021 tatcaagatt agttcggcct ctgactttcg taacgtcagt tcataccctc gcactgtcag
     7081 ttcgattgga tctcccagag gggctagttt tcggataact atttttacat ttcgcgtcaa
     7141 tcccatatcc attaaccgtc gcttcactgc gccttgtccg tggattgcta cgacgatccc
     7201 agtctctcct gcttttaatt gatcaagaga agtccagctt tcagaggaag cagtcttttt
     7261 attgacaaag atttgaccta gtatttcccg acttactgcc actcgatgac tttttaccag
     7321 taaaatggca ttgttgcctg ataaattgac gacgacgatt ttacttccgg caacgatccc
     7381 gatattttgc aaatggcgtt ttgttttttc ttctgtttcg attcgttcga cttgataaac
     7441 gaaattctgc tttgcctgcg ctaaggtctg catattgatt cctctcttgt gaaataaatc
     7501 atgtattaaa atcattcatt atatgttcta attttcttac gtgttacgca aataaatgag
     7561 aatcattctc acatagaata ctctcggtaa ttgagaatgt caattatact tgtgatatta
     7621 tgagcgaatc tttagtattg atagataaaa atgagaaaaa aatatttctt catagttttc
     7681 cacaattttt tctctattaa atttgctatt ttcccacacg atacttgttt tcagctcttt
     7741 tttcaaaaac acaaaactta gtattccaca gtttccacaa cacaatatgt agtgtttgtt
     7801 gtttgacttt tttactaaga gttttatact atctattata gataataaga atgtgttaga
     7861 ggaggcactc accatgaacg taaaaatctt ttctaaaaat aattgtatgc aatgtaagat
     7921 ggtcaaacgg tttttaacag aaaatcaaat tgcttttgaa gaagtcaata tcgatgaaca
     7981 gcctgaagca gtagactggt tgaaagcaca aggattccaa agtgttcctg tcattacttc
     8041 tgatgcagaa acagttatcg gattccgtcc tgatcagttg cgtaaacttg ccagctaaca
     8101 tgactaaaag aatcaaaacg tgatacatgg atttgaaggg cttgagacag caccagtttt
     8161 tgtcgcaaac ttccaaatcc ttttttaaag cttaaaaaca atgcaatgat ttgcaatcaa
     8221 gcatcatcaa atacgaaatg aaggttgggc gtacgtatga gtcttaaaaa tttacaagat
     8281 gtcagttatt tcaagctgaa taatgaaatc aatcggccag tcaatggtca aatccctttg
     8341 aataaggata aagaagcttt agttgccttt tttgaggaaa atgtcaaacc aaatacgatg
     8401 acttttctat ctattatgga aaaaatcaat tacttgatcg atcaagaata tatagaagca
     8461 gaattcattc aattgtattc accatcattt attgaaaagc tatatcaatt tttggacgag
     8521 caacattttt ctttcaaatc ttttatggct gcttacaaat tttattctca atatgcgttg
     8581 aaaaccaacg acggttccgc ttatctggaa acttatgaag atcgagtagc cttcaatgcg
     8641 ctttactttg cgaacgggga tgaagaatta gcacttgctc ttgcagacga aatgatccat
     8701 caacgatatc agccagctac accttctttc ttgaatgctg gacgtaaacg tcggggcgaa
     8761 ttggtgtctt gtttcttgac tcaagtcact gacgatatga actctatcgg gcgctcaatc
     8821 aactctgctt tgcaattgtc ccgtattggt ggcggcgtag ggattacttt atcaaatctt
     8881 cgtgaagccg gcgctcctat caaaggatat gagggtgctg caagtggtgt cgttccagtc
     8941 atgaaattat tcgaagatag tttcagctat tctaatcaat taggtcagcg tcaaggagct
     9001 ggcgtggttt atttgaacgt tttccatcca gacattattg ctttcctttc agcaaaaaaa
     9061 gaaaatgcag atgaaaagat tcgcgtaaaa actctttctc ttggcgtgat cgtacctgat
     9121 aaattttatg aattgacacg taaaaacgaa gatatgtatt tatttagtcc atatagtgtc
     9181 gaaaaagaat atggtgtccc attctcttat gtaaatatta cagctgagta tgatcatcta
     9241 gttgcaaatc caaacattcg caaacaaaaa atcaaagcgc gtgatcttga aaatgaaatc
     9301 tcaaaattac aacaagaatc tggctatcct tacgtaatca atattgatac agcgaaccgc
     9361 gagaatccaa tcgatggcaa gatcatcatg agtaatttat gttcagagat tttacaagtt
     9421 caaacgcctt ctgtgattaa cggcaaacag gaatatgaag tattaggaac ggatatctca
     9481 tgtaacttag gctcgacaaa tatcgtgaat ttgatgcaaa gccctaactt tggtcaatct
     9541 gtgcgtacga tgactcgtgc attgacattt attacagatg catctgatat cgatgtcgta
     9601 ccaccgatcc aaaacggaaa taagctgaat catacgattg gattaggtgc catgggctta
     9661 cacacttatc ttgcaaaaga acaaatggaa tatggctcag aggattcatt agactttaca
     9721 aatatttact tcatgctgtt gaattattgg acacttgttg aaagcaacaa tatcgctcgt
     9781 gaaagaaaac aagttttcca taattttgaa aaatcagctt atgctgacgg cacttatttc
     9841 gaaaaatacg taactggaga attccagcca aaatcagaaa aagtcaaagc attgtttgaa
     9901 ggcattttta tcccaactgc tgaagattgg aatgctttga aacaagcagt aatgaaagat
     9961 ggactgtatc atcaaaatcg cttggcagta gcaccaaatg gctcaatttc atatatcaac
    10021 gatacgagtg caagtattca tcctatcact cgcatgatcg aagaacgcca agagaagaaa
    10081 atcggtaaaa tttattaccc agcgccttat ctatccaatg agacgattcc gtattacact
    10141 tctgcttatg atatggacat gcgtaaagta atcgatgttt atgcaatggc acaacaacac
    10201 gtcgatcaag gcatgagttt gactttattc atgcgttctg aaatcccaga aggcatttat
    10261 gaatggaaga caacaacgaa gcaaacgaca cgtgatttga atattttaag acattacgct
    10321 ttccacaaag gaatcaaatc catttattac gtacgtacct ttactgatga tgcagaagaa
    10381 atcggaagca atcaatgtga aagctgcgtg atctaatatc atagatagat tcgcttattc
    10441 atattttaat taaaggagca ttctcatgtc agaaacatat tatgaagcaa taaactggaa
    10501 tgaaattgaa gatatcattg ataaatccac ttgggagaag ctgacagagc aattttggct
    10561 agatacacgt atcccacttt caaatgacct agatgattgg cgtacattgt ctcaattgga
    10621 aaaagataca gtaggacatg tatttggcgg tttgacactg ctagatactg tccaatctga
    10681 aagtggcatg gatcagttgc gtaaagatgt ccgcactccc catgaagaag ccgttttgaa
    10741 caatattcaa ttcatggaat ccgttcatgc caaaagttat tcttctattt ttagtacact
    10801 gaatacgaaa aaagaaatcg aagaaatttt tgactggaca aatacgaatc cttatctgca
    10861 aaaaaaagct gagcgtatca atgaaatcta caaacacggt acaccgctag aaaagaaaat
    10921 tgccagcgtc tttttagaaa cattcttgtt ctattcaggt ttttatactc ctctttatta
    10981 tttaggaaac aacaaattgg ctaacgtagc agagatcatc aaactaatca tccgagatga
    11041 atctgtacat ggaacctaca tcggttacaa attccaatta ggattcaacg agttacctga
    11101 agcagaacaa gaaacgttaa aagactggat gtacaatctt ctttatgagc tttacgaaaa
    11161 cgaagaacgt tatacagaag aattatacga tccaatcggc tggacagaag aagtcaagac
    11221 attcctacgc tataatgcca acaaagcatt gatgaaccta gggatggatc cactctttcc
    11281 agatactgcc aatgatgtca acccaatcgt aatgaacggg atttcaacag gcacaagcaa
    11341 ccatgacttc ttctcacaag tcggaaacgg ctatttgtta ggtcatgtag aagcgatgaa
    11401 agacgacgat tatttgattg gattggaata aggatttatt ttaattcaaa aatttttcga
    11461 aaaataaaaa gagtttagcg tacagatatt ttaaatgtac actgaactct tttttatgct
    11521 tattcacttg tttcttccag cacttttgga ctgtctaact tttccgaaac atgttccatt
    11581 cttctcaata aatagatttt ccggatattt aatattatcg tcttaatcac tgcatagaca
    11641 ggtatgcaaa tcagcatgcc taatattcct gcgacacttc ctgctccaat caataccaag
    11701 atgatcgtca aaggatggat attcattgat ttcccaaata acaacggctt gatcaaattt
    11761 ccatctaatt gttggatcac taaaatcgac aacgccatat acaaagcttg aaatggagag
    11821 ataaacaaac cgacaatgat tgctggtacc gctccaataa aagggccgac atacgggata
    11881 atattcgtga ttccgcaaat gaccgcaagc aacaagctat aaggctgatc gaatattctc
    11941 attgccaaaa agctcattac accaataatc aaagcatcta agatcgtact gctgatatag
    12001 gcagataatg tttcgtttaa ttcttttacc gtttggcgta actctttacg aatcgctttc
    12061 ggaaagaagt gagacaacgc atcaataaat ttgtgcccat ccttgaacat aaatagcaaa
    12121 ataaatggaa cggtaaataa caaaataaaa aattgcatca taaatgaaat gatttttgag
    12181 acacttgcag ataaactgac taatatgata ttgaacaaat tactaagaga taaatttgct
    12241 gatgctaatt gttcttccaa attgaaattt ttaaactctt cccgctgcgc caattcattt
    12301 aaccatttgc ttgtttcatc tgcatacatt ggcaagctct gggtcaattg aattgtctgt
    12361 tccattaact gaggcactac attcatgaca gttaaaacaa ctaaaatgat tacgaccaaa
    12421 aacgtcaata aaaaaccgaa cactctcggg atccgatgct tttcgagaaa aacaacgatt
    12481 ggatcgaaca tataatagat aaaaccagca acaagcaatg gtaaaaaaac tgccgaaagc
    12541 attttcaata ttggactaaa aatattcggc atttgtaaaa taaagtacac gccgatgatc
    12601 gtgagtacta gctccactgt ccagaaaaat aattttgatt gtttaaaacg tgattgcata
    12661 catctcctcc aaggtccttt gatacttcta ttctatcatt taacaaagag ttacgcgctg
    12721 agattttcat ttgcttaaaa aaaacaaaaa aaataaacat attttaatga gtagacgagt
    12781 acttaaacaa aaacacggtt tggagtagtg acaaaaatta tactgcagtc cctttttcca
    12841 aataaacggt gttcaaaagc ttgcatcttc gtaataagcc cagccaaacg aaaaatataa
    12901 ggaactattt ttatttggct gggcttatta cttgatgtag gacatctatt ttacaacctc
    12961 agatcattta ttgcgcaata ctgactaaat gcaactttgt gatatttttg tgtacggaca
    13021 actcctcgat cacatcggca taagtgagcc cttttggaag atcaatcgta tagatatttt
    13081 tgtacactcg gtagtcatca acgaatttta cgtcaaaatt cacatcttcg attacgatat
    13141 ttttctcttc aaaataatcg gtaagaaaat ctttggtttc ttttctatgg ataaattgga
    13201 tttctaactt tttagtcgta ggtacatgga tcacgcgttt cactactgtg agtgcaatag
    13261 caatcccgat aaaactgatg atcgcaatgg aatagtaacc catgccgatg gcgatcccta
    13321 atccagcaat tgcccataaa gaagcggcag tcgtaagtcc cgtcaccgat tgtttcgtga
    13381 cgatgatcgt tcccgcgcct aagaaaccga caccactcac cacttgagca atcagccttg
    13441 cttcatctga acgaatcgtt ccagcaagct gtggatactc taaagcatct cttaatgcac
    13501 cactagcgat ctcaacttgg atcaatgcaa tgatacatgc tcccatacaa actaaaatat
    13561 gcgtacgcat acctgctgga cgatttttat attgtcgttc aaaaccgatt gctccgccaa
    13621 atagcatagc gatccctaaa cgtaaaataa tctctggaat tgtcaatacc atatcattca
    13681 tctaaacacc ctccagtcca atatatcatg tctgaaagga gcagacaaaa aaagtgcaat
    13741 ttttacctct tacgaaaaaa ggactgtgac aaaatttttg tcatagtcct ttttatgaat
    13801 aaacggtgtt caatcagctg accctcggta ttactcaaca aacggcaaaa tatggggagc
    13861 tatttttcct ttgtcttctc taatactcag gtcaaaacgc tgatttcaca acctctttag
    13921 cctactattg aaaattacaa cttacttgct gcttcttcat cgatgatcac aacaacattg
    13981 tcgtgatttt gcaatacact tgctggcaaa tgattcgtta caggtccttc aatcatccct
    14041 ttgattgcat cagctttgct ttcgccaaat gccatcaaga cgatttcttt acttttcata
    14101 attgaaccga ttcccattga aacagctttt gttggtacat cttctgcttt atcgaagaat
    14161 cgtttgtttg cattgattgt tgattctgtt aagtcaacta ctgaagtcgt tccgtcaaat
    14221 gaagcaccag gttcattgaa accgatatgt ccattttgcc cgattcctaa gatttggata
    14281 tcgattggat gtgcatcgat gatttgatca tactttttgc atgctgcttc cagatcttct
    14341 gcttttccat ctggtacaaa cgtttctttg aatggttttt tatcaaataa ttggacatcc
    14401 atgaaatgac ggtagctttg ttcatctgaa ccgcctagtc caacgtactc atctaagttt
    14461 acagaagtca tttcactgaa atccaaatcg cttgaagtca tttctttata taaagtaact
    14521 ggtgtgcttc ctgttgccaa tcctaatact tttgcacctt tgttcatacc ttctttgatg
    14581 atttcaaatg ctttttttcc gccttcttca gcgtttttta cacgaatgat ttccatagtg
    14641 attgctcctt tatattttgg tatagcccaa ttatagcatt ctttttttct ttttacaata
    14701 attagtatag accaaattgc atttttttca ttttcttgac aataaatagg aatccgtgtt
    14761 aggtttcata agatggttca atcaaacaat ccatgttgca tgaaaaccaa gcaagtgatt
    14821 cactgtggac gctttatgtc tgcagttttt ttatgaaaaa ggagttatta tgagtatact
    14881 gacattaaac aacattaccc agcaatttgg cgacaaaatc ctctatgaag gtgtcgatct
    14941 gcaattaaat gctggcgaac atcttgggct gattggacaa aacggagccg gtaaaagtac
    15001 attgattaaa atcatcactg gtgagatctt accagatgac ggacagatcc attggcaaaa
    15061 aaatattcac aaaggttacc tcgatcaata tgtggaagtc gatgaaacat taacaatcga
    15121 agaatttcta aaaactgcgt acgcaaaaga atacgaaaag gaagaaaaaa ttgcttttct
    15181 ttaccaaaca tatagcgaga ccttcgatga ttccttgctg gaaaaagctg ggaaactcca
    15241 aacggaatta gaccaaggag tgttttatca aatcgacacc ttagttgctg agatgagcag
    15301 cgggttgggg atcgatgtct taggtttaga ttcactgttg aaaaatctaa gtggtggaca
    15361 acgttcaaag gtgattttag caaaactact tttagaaaac ccagatgttc tgatcttaga
    15421 tgaaccaacc aatcacttag atgatcagca tatccaatgg ctgacgcagt ttcttcagga
    15481 ttttgacggt gcgtatatcg tgatttccca tgaccaagaa tttcttgatc agatcaccac
    15541 ccatatcgca gacatcgagt ttggaaagat tactaagtat actggccatc taaaacaagc
    15601 attgaaacaa aaggaacaaa atcgcgaaag ctatttgcgc caatatcatg ctcaacaaaa
    15661 acaaatcgaa aagaccgaag cttatattcg aaaatacaaa gccggttccc gatcaacgat
    15721 ggcaaaaagc cgacaaaaac aattagataa aatcgaacgg ctgacgcctc cagcttcagc
    15781 aagtaaagct gtctttgatt ttccattctc gcctattgtc acgacattag cagtggaaac
    15841 aactgattta gtcatcggat atgatcgacc gctacttgca cccatcaatt tgaccatccg
    15901 ctttggtgaa aatattgcca ttcgaggctt caacgggatt ggaaaatcta ctttattaaa
    15961 aacactgatc ggtgagattg aaaaaataag tggcgatttc cacttcccag ataacacaaa
    16021 aatcaattac ttttcacaag atctgtactg ggaaaatcca ttagaaacac cactacaata
    16081 tttaagcagt caatttccaa aagcgactat caaagaatta cgtcgccaac tagctaaagc
    16141 agggttagtt aatcaattag caagtgaacc tctcactaca ttaagtggtg gggaacaaac
    16201 taaggtgaaa cttgcacagt tgacaatgaa ccaaggtaat ttactgattc tcgatgaacc
    16261 gacgaaccat atcgatcaag aaacgaaaga aagcttacaa gacgggatac agaaatttcc
    16321 aggaacagta atcatcgtct ctcatgaaca agaattttat caagatcttg tgcaacgagt
    16381 cattgagatt gaaccaaaat aaacgtttga ttgaatagtt gaaaaaaagg agctgtgaca
    16441 aaagttttgt cacaactcct tcttctgaat aaatggtggc caattagctg accttcggta
    16501 ttagctcaaa aaacggcaaa atatgaggag ctatttttat ttggctattc ttattacttg
    16561 atgcagaaca gcttttcaca acctcttttc gtctattttt tattttcgag atttctcttt
    16621 cctttaaaaa caactgtttg aaagaagaac gcacatcctt gaaaaacaag cccgctaacc
    16681 gttacaccta accagccata ctgctgccac attgtcgtcc ctagcaatga accagccgca
    16741 cctccaataa agtacataaa cataaataca gtattatttc ggttgcttgc tgcttcacct
    16801 agcccttgta ctcgtgtttg attggcgact tgaccaaatt gattcccgac gtctaataaa
    16861 ataatagcta aaagcaaaat ggccaaatgg cttccgccaa ccaataaagc aataaagctt
    16921 attgtttgca taacaagtcc catatacacg atttttcttt cagaataaga atctgccagt
    16981 cgtcccacga taggtgcagc aactgctcca gacaaaccaa aaatcgctaa tatccctgct
    17041 tcgaatgttc cccaattgta ggcgggactg ctgatataaa aaatcaacgt agaccaaaag
    17101 atcgaaaatg ttccaaacat gaaaaatcca ctgactgccg cttctcttaa tagacgttga
    17161 gaagcaacta aatgcggcaa acttttaagt gaatcaaggt atgaaacagc tgtactactt
    17221 tgctggacag aacgcggcat tttccagtaa agcagaagtg taagaattcc gactgcacat
    17281 aaagcaaata aatagatcat ccgccaagaa aaaagactgg caacaatacc agataccgtc
    17341 cgagaaagca aaatccccac gagtaaaccg ctaagcaatg tacccatgac cttccctctt
    17401 gcttttggtc ccgctagtac cgcaccgtaa ggaatgatga tttgcggcac gatcgaaagt
    17461 aatcctatta aaaaagaagc gcatgcaaat aacgtaaacg aaggcgcaaa aaaagcggcc
    17521 aacagagaga cggcagataa cgctgccatt cgaatgatca attttggacg atccaccacg
    17581 tcccctaaag gaactaaaaa cagtaaccct aacgcatagc ctaattgggt caacatcgtc
    17641 aatatcccga ctgcacttgt gctaacagat aaactagctg ctacttttgt tccaatcggc
    17701 tggatgtaat acatattggc aacaacaacg ccacaagtga ttgataatag taaaataagc
    17761 gaagttgtta aaggtttttt ttcattactc ttcattgatt tcccccattt cctatcttag
    17821 tttacaactt atggaaaaga ttggaaatag aaaaatacaa ctattcgcca cacttattat
    17881 ttgtgctttt gttttccttg ttttttcttg cgatctttat aaatgaagac aactaaccct
    17941 actagcacag taacacttgc aaccaacgtg actgcagttt tgttatatcc gtgaaacaga
    18001 gcttctccac cacacgcata caacacagac gaagggatga ctccaatcac gatcgacaat
    18061 atgatttgct gtagaggcaa tttcatcgca tctgcagcaa aattgaccat agaagatgga
    18121 ataaccggta tcatatagcc taacattaca cctatttttg gatgtttcat ttgtcggatt
    18181 gcttgcaccc aatgattcgt ttcggttttc ttatctaaaa attttaagcg atgcataagg
    18241 aaaattgcag tgatgttacc aagagcatta cccaaaatgt tgatgatcgc acctgtcagt
    18301 ggtccaaagc tcaaaccagc aatgactcca actactgaag taggaatacc tggaacagca
    18361 cacatcaccg ctgtcaatac gatcaagagt atcgatgcaa aagtcccatg cgaacgaata
    18421 tgtgcgagta gctctgcctt actcgctctc ggatttaaaa ataattggat gtctgtctga
    18481 tattctaaat acaaccgata gcccactacc agtatcagaa tgattcccaa cgagatcaat
    18541 atcaatcgga ttattttctt tttcatattt cctcctgaaa aaaatctata ttcattaaat
    18601 aatagtttaa cataaaaaca aaaaagcacc gaaatccaac ataggatctc agtgctttta
    18661 aatagtaaaa attatttaac agttacagat gcgccaactt cttcaagttt agctttcaat
    18721 tcttcagctt cttctttaga tacgccttct ttaactggtg caggagcgcc atcaactaca
    18781 gctttagctt ctttcaagcc taaaccagtt gcttcacgaa ctgctttgat aactttaact
    18841 ttttggtctc cagctgcagt taattctaca gtgaattctg tttgttcttc agcagcagca
    18901 gcgccaccag cagcagctac agctacagga gcagcagcag atacgccaaa ttcttcttca
    18961 atagctttaa ctaggtcgtt aagttctagg atagttgctt cttttaactc agcaacgatg
    19021 ttttcaatgt tcaatgccat tataatttcc tccgtttaat tgttcttttt tatttaatgc
    19081 tttatgcggc aattaagctg catctccatc ttctttgtct gccactgctt tgacagcgta
    19141 agccacgttg cggactggcg cttgtagtac agatagcaac atagatagaa gtccttcgcg
    19201 atttggcaat tttgccaatg ctgtgatttc ttccaatgaa gagactttac cttcgatgat
    19261 accgcctttg atttctaatg cttttgcttc tttagcaaat tcgtccatga ttttcgctgg
    19321 tgcgactaca tcttcgttac tgaatgcaac agctgtaggt ccagtgaata cttcgtccat
    19381 gccttcaagg ccagcttttt tagctgcacg tgaaagaata gaatttttga taactttcat
    19441 ttctacgtta gcatcacgaa gttgtttacg taaacgtgtt acttcatcaa ctgttaatcc
    19501 gcggtagtca acgactacta ctgatgcagc tgctgtaaat ttttcgctta cttcatcaac
    19561 gattgctgct ttttttgcga taattgcttc actcattttt gagtttcacc tcctgaattt
    19621 tgatggaaga gttttttgca ctgaaaaggc aagtcacttt tatagttact ttgctcaacc
    19681 agtattattc atcacctatt cacttcgttc atagtgattc tactcgcttt gctcgaaagg
    19741 aaactccttt cgctttgctc aaccagtatt gttcatcatc tcttcacttc gttcatagtg
    19801 attcacaata aaaaactcta tgccaccgca gacatagagg gactattgaa tatattcaat
    19861 aaacgtcctc ggtaggaaat taaggcttga tgccacctac tgtcttcggt acaagttaat
    19921 tattaacctc aactagctta gcatgctaaa ctagttgagt caagaattat ttttcagttg
    19981 gatcaatcaa cattctaata tttaattaga aagaagcttg gtcaacatgg ataccaggtc
    20041 caaatgttgt tgtgatcgtt acatttttca tgtattgacc tttagctgct gatggtttag
    20101 cttttagcaa tacatcatgg attgctgcaa agttttcaac taatttttca tcgtcaaatg
    20161 aaacttttcc gattggtaca tggatatttc cagctttgtc cacacggtaa gttactttac
    20221 cagctttcac ttcgtttact gcttttgtta cgtccattgt aactgtacca gttttagggt
    20281 ttggcattaa gccttttggt cctaatactc gtcctagacg acctacagta gccatcatat
    20341 caggtgttgc aacaactaca tcaaagtcaa accatccgtt ttggattttt tgaaccatgt
    20401 cgtcgtcacc tacgaaatct gcaccagctg cttcagcttc ttttgctttg tctcctttag
    20461 caaaaactaa aacagtttgt gttttaccag ttccgtttgg taatacaaca gcaccacgga
    20521 tttgttggtc tgctttttta gggtctacgt tcaaacggta tgcaacttca actgttgcat
    20581 caaattttgc aatgtttgtt tcttttgcta aagctactgc ttcggcaaca ggataaactt
    20641 tacttgaatc aacttttttt aatgcatctt gcattttttt gctcttttta gccattttgg
    20701 ttcctccttg attgtggtta taacggttgt acctcccacg tgttctatat ctttcaatat
    20761 agagccaact gagaagctgc tgtcttaggg tgtatagatt attctacagt gatccccatg
    20821 cttcgtgcag ttccttcaac catgcgcatt gctgcttcaa cgttagctgc gtttaggtct
    20881 tccattttta attcagcgat ttctcttact tgatcgctag aaactttcgc aactttattt
    20941 ttgtttggtt cgcctgaacc tttttcgatt ttagctgctt ttttcaatag aacagcagct
    21001 ggcggtgttt ttgtaacgaa tgtgaatgaa cggtcttcgt atacagaaat aacaaccgga
    21061 atgatcaaac ctgcttgatc agctgtacga gcgttgaatt cttttgtgaa tcccatgata
    21121 ttgatacccg cttgacctag tgcaggacct actggtggag ctggagttgc tttacctgca
    21181 ggaatttgca atttaacgat tttttctact ttctttgcca cgagacatac ctccttaagt
    21241 ccgtgatgtg gttaattgga gatgcatatt tctcctccca cgttttatct gatctgaaaa
    21301 aatccagaca aataatgtgc aaaacaatgc acactggtac agtatagcaa atttatttgt
    21361 tttttcaaga tttttttctg ctatttttac attttgcttg aataaacgct aaatttattc
    21421 taaaaaaaca acaatttttc gttctcattt cttcttttat gttacacttg cgctagcatg
    21481 gttcaacaga tgaaagcaaa caataacacg gttttttaga cccttgtaca aagattccta
    21541 cataacaaaa tgaggtggag aaaatgaaag atttaaggta tagaagtgtc tttgatatta
    21601 ttggacctgt gatgattggt ccaagtagct ctcacactgc cggagctgct agaatcggga
    21661 aaatcatacg tagtatattt ggcgaacagc ctgattctgt agatatttat ctatatgaat
    21721 catttgcaaa aacctatcgg gggcacggaa cagatattgc attagttggc ggcctgttag
    21781 ggatggaacc cgatgatgaa cgtttagctg attctttgaa attggcatat gaagccggta
    21841 tggaagtctt gtttgttcct aagagtgaaa aagccgaaca tccaaactct gtaaagatgc
    21901 ttctaaaaaa aggaacacga aagctttcag tcactggcat ttcgatcggc ggtggcaata
    21961 tccagatttc tgaattgaat ggattcaaaa tttctttaag catgggtact cctaccttga
    22021 tcgttgtcca ccaagatgtt ccaggtatga ttgctaaagt gactagtata ctttctgaga
    22081 ccaatacgaa tatcggcact atgacagtga cacgggaatc aaaaggtgaa aatgccatta
    22141 tgataattga agtagatgat cctcaagtag aagaaacggt aaaaagactc aaacagctgc
    22201 caaatatcga cagcgtaaat tatttcgatt aaggagctaa aaaaatgttt ttatcgattg
    22261 aagaattagt agatcaagca aaagcttatc caagtgtagc agaattgatg attgcagttg
    22321 aaatggaaat gagtggccgt tcccgtagtc acatcatcga gatcatggaa aaaaacctct
    22381 cagtaatgga acaatcaatc aacgagggaa aagaaggtgt tacctccgtc acaggaatta
    22441 ccggtggtga tgcaaaaaaa ttagatgatt atttaaatga ggcagacttt ttaagtggag
    22501 aaacaatctt aaaagctgta cgcaatgctg tagctgttaa tgaagtcaat gcaaaaatgg
    22561 ggttgatttg tgctacaccg actgccggaa gcgcgggtgt tgttcctggt gtgctgatgg
    22621 ctgtccgaga tcggcttgat ttgacacatg aacagcaatt ggaattctta tttaccgctg
    22681 gcgctttcgg gttagtcatc gcaaacaacg cttctatcag tggtgcagaa gggggatgtc
    22741 aagcagaagt tggttctgca agtgcaatgg cagcagcagc tttggtttgt gcaaaaaatg
    22801 ggactcctca tcaagcagca caagcagtag ccattacatt gaaaaatatg atgggactga
    22861 tttgcgatcc cgttgcaggc ttagttgaag tcccgtgtgt caaacgtaat gctttaggct
    22921 cttctcaagc ttttatctct gctgatatgg cattagcggg tatcgaaagt gtcattccgc
    22981 cggatgaagt cgttgctgca atgtaccaag tcggacgtca aatgccgtcg attttcaaag
    23041 aaacagcaga aggtggctta gctgttacgc caactgctaa acgacttgcg gaagagattt
    23101 taaataagag ggcgtgataa agctccttaa tttcgagtat taagagatta gttatcttca
    23161 taacaaaaaa cgaagagagg actaattgcc cttctcttcg ttttttatta atccatcaat
    23221 ttcgtatttt ttacttcatc gatcgttttt gttcctgcaa gttgcatcgt gatcgacaat
    23281 tctttgttca ggtgttcaaa aacagatgtt actccttctg ccccacctag attcaaccca
    23341 taaatcactg gtcggccaat cgcaactaag tcagcaccac ttgccagagc tttgaagaca
    23401 tgctctcctc gacggacacc agagtcgaaa atcactggta cacgtttgtt gactacttcg
    23461 gcaatttttg gcaatacttc aaaggacgca ggacctccat ctagttgacg tccaccatga
    23521 ttggataccc agataccatc tgcccctgca gagatagcga ttactgcatc ttctggcgat
    23581 tgtattcctt ttacgataac tggaagatgg gtgatttctt tgattgtttt gatgtcatct
    23641 ggtgtcaagc cttgttttgc agctgcataa atttctgcga ttccttttcc ttcgccatta
    23701 ccacttgcac tttgttcgct atacgcagct aagttaggca ttggtaatgg aaattggaat
    23761 tgattgatga cgtcttcttc acggtaacca ccgagcgttg aatctgccgt taaaatgata
    23821 gctttagccc ctgcttctac tgctttgttc aaaatgaact cattgaatct gtcgtcttta
    23881 ctcatatata attggaaaaa ctgcggtgcg cctggcgctg catcggctgc gtctttgatt
    23941 gtcgtatttg cataagtgct gatggaaaag attgatccag cagcagctac tcctttagct
    24001 gtatcagctt cccctttttc atgtgccaat ccttgggccg ctgatggcgc ttggataatc
    24061 ggtgtatcta attcaattcc gaaaacagat gtatgcaaat cggctgagtc gattccgcgt
    24121 aatatccgtg gcatgatttt ctttgtatta aaagaagtcg tattttcttt catcgtccac
    24181 tcatcttcag aaccaccgcg aatatagcca aaagctcctg cttccatgcg ctcttttaca
    24241 cgaccttcta gttaggcaat gtttacaata tcgattggat gttcttcatt acttgtatga
    24301 taagtcatag tagatccttc tttcatcttc ttgattacat ataaatcata ctaaacttac
    24361 aaaaagtaag caaacattta ctctcagata cttttatcgc tattctttcc aaataaatcg
    24421 acttacaagc tgatctactt cttggttttc atgaagcata ctatgagagg cattttcacc
    24481 tgtcactact ttttcctcta tttcatttcc gcgttgcttc aacaacgcat agaccgctaa
    24541 tgcactgttt aacggtactg ttccatcact caagtctgtt tcatctagtt gaccagcaat
    24601 caagaaaaat cgagtggtta taggaatatt accgatcatt tgctggtaat cttgatagcg
    24661 gttagaaacg acagcaggcc cctttgctaa ctcatctgtc agagactgcg cactatcatc
    24721 gacaaaatca ttaaaaggcg cgccaattgc tgttaatgtc tgaacttgtg gcaatgacgt
    24781 atcctgtcca tacgttccta aatagcgtag tgcactaact cctcccatgg aatgccccac
    24841 tatgttgact ttatctatgc cgtagttttc tttcagatac aaaagacaag ttttgatcca
    24901 ttctgcttga ttccactcgt tatttttatt atcttcaaac aatacttgga ccatcggatt
    24961 gtctgctttt ccagacaaat ctcctgttgc ttgtatttgt ccatctgttc ctactgttaa
    25021 aaccaattct tttactgctt gctgattttt ttccaaacgc cgcaacatcc ctttaaagga
    25081 attttctgtc ccgctgtagc cgtggaaaaa aagtgttgga atagtggttt tttctatcac
    25141 tttactggat tctgccgttt tttctttatc agaaacttca gtagtattta tcttcgtcgt
    25201 ctggcaacca cttaacgcaa acaaactgat aaggactaaa gctgctgcag ttgcacgttt
    25261 gcataaattt ttgattggtt ctgtcataca cctcttcctt tccatataga aaaaagaaag
    25321 acttgaaagg ccaatcatat gatcgacatt tcaagtctct ttattaatcg attttatcta
    25381 cttgatcgaa atcaagttct gtactggttt cacgtccaaa catatcgatg ttgactttca
    25441 atttttcttt ttctccgtca acttctgtta cttgtccttc taatccagca aatgcgcctt
    25501 cgatgatttt cacgacttcg ccaacttcta cttctagatc attttgtcgc aggctcatac
    25561 cgatagaacg caagatatga ttgacttctt ctggaagtaa tggtgctggt ttgcttccgg
    25621 ctccgtgtga accaacgaat cctgtcacac caggtgtgtt gcggacaaca taccatgaag
    25681 cgtctgtcat gatcatttca acaagtacgt atcctgggaa tgttttatga acgatttctt
    25741 tttctttccc atttttgact tcagtttctt tttcttcagg aacaacgaca cggaaaataa
    25801 agtcggccat tttcatactt tgcgcacgag attcgatatt tgcttttact ttgttttcat
    25861 atcctgaata tgtatgcaag acataccagt ttttttcaaa agattccatt gaaggctcct
    25921 catttcattg taaaataaaa aaacctcagt acacccgaag tttttctttc gcttcattat
    25981 agcactaaaa gtacgttgtg gctagcgagt tattttaaaa tccagccaaa taagctttgg
    26041 atggctgtat ccattacgta aaacatcaat ccgaaaataa tagaagtttc gatgactact
    26101 aacgtatctt tacggagttg ttttctgctt ggccaagtta cttgtttgat ttcatctttt
    26161 acgcttttta gaaatttcat gagacaacct cccttgctac tgtttatttc gtttctttat
    26221 gtaatgtata ttgattacaa tatttacaaa acttattgat ttccaaacgt tcgccgcttt
    26281 ttccttcact gactgatttt gtgtagttgc gagacccaca aacggaacaa gcgagtgctg
    26341 cttttttctt tgccatttac ttctgccccc ttgctgagaa tcagacttat acttcataac
    26401 agtatcacgt aactttttac ttgtcaattt gtgaaataaa ctatctattg ttttggataa
    26461 aattatgata tgattaattt gaaattaagc taaaatgaga caattggagg tatagcgatg
    26521 ctattaaaaa cacttgtata taagaaaaaa gatctgacaa ccgttacaga gactagtaca
    26581 ttagaagaag cattaaaaat tttagaaact tccggatacc gttgtgtgcc gatcttagat
    26641 tcttcaggta atattttccg tggaaacatt tacaaaatgc atatctatcg tcataaagca
    26701 aatggcggtg atatgacgct tcctgtcact catcttttga aaaacgctac aaagttcatt
    26761 tacctggata cttctttctt caaagtattc tttacgatca aagagcttcc ttatattgct
    26821 gtccttgaca gtgaaaatca tttttatggt attttgacac atagtacgtt gcttaatatg
    26881 ctctctcaat cttggagcgt agaacaagga agctatgtac tgacaattgc ttccacagga
    26941 aagcaaggag atttggctgc tattgcaaaa atcatttcaa aacacagcag cattgccagc
    27001 tgtattactc ttgatatcga aaaagacgaa tacattcgac gtacattgat cacgctaccg
    27061 gctaacactg aagcgaaagt atgtgaaacg atcgtcaacc acttagagaa aaagaattat
    27121 aaagtggtag aaattgaaaa tttgcaaaac caagaagcat aatcaaaaag cgaaaaaaga
    27181 aaacagctaa tctgctttct tttttcgctt tttataatta gacacgcttt tcaaaatcaa
    27241 gcgcgcgatc taaataatac atcaatggtg ctgaaacagc catgacgata aactcactta
    27301 atgcaagcgt gccataggtt gtccaaaatg cttggccacc tgttggaact aacatataag
    27361 caatcagtcc catgctgatg gtaaagaaga tcgtgttcaa tactaaacgc atctttacat
    27421 ttttcacttt gttttgcaat aaagctgtca ggcctaaaga aagcaatgtt tggccgccac
    27481 caaaaaccac atccatgata ccaaaaccaa agaaggcatt atagacgatg accccgccta
    27541 aaactcccca aagcaattta cggttaaaga caactaaatg attcaaactt tctgaaatac
    27601 gaaactggat cggccctgag gatacaggcg caactaaaat agttaacgct agataaagag
    27661 ccatgatgat gccatttaca acgattacgg ataaccgttg tctgcttttc gttttttgca
    27721 ttttctctcc tcttttctcc tagttttttt aacgcgggat ggttgatgaa ccgcggttat
    27781 gtgaaacaaa caataattat aacagacttt tcatttattt caatccgtct tatctgcttt
    27841 taaaaaagca aaagcttaaa caatgtaaac gtttgctaaa aatcgtggga tatggtataa
    27901 agtaagtgta aggggtcata agaattccca gtaaagcaga cgaagtagga tctgcaagaa
    27961 cagaattgtt gtgaaactta aataaataaa aaaggatgtg ggaatttgag caacctctta
    28021 ctagagaacg gtctagcaag cagctgaaga tctaagtgga catgccgaac cttggaactt
    28081 ctccttacgt aagtaatgga tcatgcgctt gctaggctt
//
LOCUS       DAYYKN010000020        28076 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000020 DAYYKN010000000
VERSION     DAYYKN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.020"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..664)
                     /locus_tag="ACHBB7_000755"
     CDS             complement(83..664)
                     /locus_tag="ACHBB7_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9208728.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(847..1473)
                     /locus_tag="ACHBB7_000756"
     CDS             complement(847..1473)
                     /locus_tag="ACHBB7_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM9208729.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1495..2826)
                     /locus_tag="ACHBB7_000757"
     CDS             complement(1495..2826)
                     /locus_tag="ACHBB7_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM9208730.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2981..3382)
                     /locus_tag="ACHBB7_000758"
     CDS             complement(2981..3382)
                     /locus_tag="ACHBB7_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9208731.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3468..3674)
                     /locus_tag="ACHBB7_000759"
     CDS             complement(3468..3674)
                     /locus_tag="ACHBB7_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208732.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3769..5271)
                     /locus_tag="ACHBB7_000760"
     CDS             complement(3769..5271)
                     /locus_tag="ACHBB7_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM9208733.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5289..5744)
                     /gene="argR"
                     /locus_tag="ACHBB7_000761"
     CDS             complement(5289..5744)
                     /gene="argR"
                     /locus_tag="ACHBB7_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9208734.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5842..6459)
                     /locus_tag="ACHBB7_000762"
     CDS             complement(5842..6459)
                     /locus_tag="ACHBB7_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9208735.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6680..8053
                     /locus_tag="ACHBB7_000763"
     CDS             6680..8053
                     /locus_tag="ACHBB7_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9208736.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8128..9240
                     /locus_tag="ACHBB7_000764"
     CDS             8128..9240
                     /locus_tag="ACHBB7_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9208737.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9312..10949)
                     /locus_tag="ACHBB7_000765"
     CDS             complement(9312..10949)
                     /locus_tag="ACHBB7_000765"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM9208738.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11117..11725)
                     /locus_tag="ACHBB7_000766"
     CDS             complement(11117..11725)
                     /locus_tag="ACHBB7_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9208739.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12109..13260)
                     /gene="queG"
                     /locus_tag="ACHBB7_000767"
     CDS             complement(12109..13260)
                     /gene="queG"
                     /locus_tag="ACHBB7_000767"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM9208740.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13279..14031)
                     /locus_tag="ACHBB7_000768"
     CDS             complement(13279..14031)
                     /locus_tag="ACHBB7_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208741.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14172..15122)
                     /locus_tag="ACHBB7_000769"
     CDS             complement(14172..15122)
                     /locus_tag="ACHBB7_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM9208742.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15123..15962)
                     /locus_tag="ACHBB7_000770"
     CDS             complement(15123..15962)
                     /locus_tag="ACHBB7_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9208743.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15959..16705)
                     /locus_tag="ACHBB7_000771"
     CDS             complement(15959..16705)
                     /locus_tag="ACHBB7_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9208744.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16943..18313
                     /locus_tag="ACHBB7_000772"
     CDS             16943..18313
                     /locus_tag="ACHBB7_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9208745.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18368..19264)
                     /locus_tag="ACHBB7_000773"
     CDS             complement(18368..19264)
                     /locus_tag="ACHBB7_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9208746.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19378..19548)
                     /locus_tag="ACHBB7_000774"
     CDS             complement(19378..19548)
                     /locus_tag="ACHBB7_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208747.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(19545..20222)
                     /locus_tag="ACHBB7_000775"
     CDS             complement(19545..20222)
                     /locus_tag="ACHBB7_000775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9208748.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20313..21218)
                     /locus_tag="ACHBB7_000776"
     CDS             complement(20313..21218)
                     /locus_tag="ACHBB7_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM9208749.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21393..22817
                     /locus_tag="ACHBB7_000777"
     CDS             21393..22817
                     /locus_tag="ACHBB7_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM9208750.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            22863..23894
                     /locus_tag="ACHBB7_000778"
     CDS             22863..23894
                     /locus_tag="ACHBB7_000778"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9208751.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23901..25088
                     /locus_tag="ACHBB7_000779"
     CDS             23901..25088
                     /locus_tag="ACHBB7_000779"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM9208752.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25244..26626
                     /locus_tag="ACHBB7_000780"
     CDS             25244..26626
                     /locus_tag="ACHBB7_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM9208753.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26663..26983
                     /locus_tag="ACHBB7_000781"
     CDS             26663..26983
                     /locus_tag="ACHBB7_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208754.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(27183..27851)
                     /locus_tag="ACHBB7_000782"
     CDS             complement(27183..27851)
                     /locus_tag="ACHBB7_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM9208755.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7814 a         5745 c         5154 g         9363 t
ORIGIN      
        1 tttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca
       61 cttctgtcac aacctctaat tattaaagaa ccttttgcga agttaacaat ctttctttga
      121 tcaattgaaa attctgccta agtagtgata gatcctgtgt gactttcaac aataagatac
      181 ttccctggat ttcagcaatc aacgctttgg cttgacggtc tgcttcttca tcggacagtt
      241 tttcttcaga aagtgccagg atattttgtt tcgtatactt gacccattga ttgactaatt
      301 ctagtagcaa cagacggtat tcttcatcca ctgtactcaa ctcaataatc aaattaccta
      361 caggacagcc atgataagtg atctgtgtct catgatcagc gaaaatccag tccaacattt
      421 cttctaatgc ttcttctggc tgttttccag catttaagat gtcttcgaac aattctgtct
      481 gccatacgtc catatgcgca tggatcaccg ctagacaaac ttcttttttt gaagaaaagt
      541 aataataaaa ttgacctttt cctgtatcag aagctgctag aatatctcgg attgacgttt
      601 catgatagcc ttttttataa ataagatctt tggtcgcttc caagatcttt tcctttgttt
      661 tcaatgatat tctccttttt catttctcat agtcctatat tatacagtaa attcatcatt
      721 ttttcttctt aatcgtttaa aaaagactgt gccaaattat ctgacacagc cctcactata
      781 aaactgttct catatagcta gatgttcatt gattcaagaa ctgcgaggac aaaagcaatt
      841 cctatcttat gatagttgct ctaatacttc ctctgttgtc atcacttgat ctgcaataaa
      901 gccaaagttc gtcaacgctg catccattgc ttcttggcca ggagcaccaa ttgcatctgt
      961 cgctaccaca acattgaatc cactctctac caattcacgc atatgggaat cgctacacaa
     1021 gtttccgttc attccaccaa gaattactgt gtcgattcca ttttttctca attggagcgc
     1081 caaatcattg ctttctggtc caaagatttt atgaggagag ctgatgatcg tattttcatc
     1141 caaataaggt ttcaattctt cgataaaatc ggctcctgaa ccttcttcca ctgattcata
     1201 ttgacttttt ctagcaaaca tctgctgctc aagcatcata tgttctcctg caccgccaaa
     1261 ttgccattta tggtcatgtg ggtaaaaata atgaggagaa ataaagagtt tgtatccttt
     1321 ttcttttgct tgttttacca tttcaatcat attttcgatc gtattgtatt tttctaagat
     1381 atcttttgtc aaaccaaagc caccgcctgc tggtttcaag aactcgactt gcgggtcagt
     1441 gagaactact gctgtatttt tattattgaa catggtggat tctccattcg ttatttattt
     1501 taatacatca tttagagctt cactcattgt cggatgagaa tagatttgat ctctcaataa
     1561 cgtataagga agttttccat tcatagctag cgaaatcaag ttgatgactt catgagaatc
     1621 ttccgcatag atacttgccc ctagaatcaa gtcagtttca gggtcgacta aaactttgaa
     1681 tacaccttta ggatcttcta atacttgtgc tttagggaca ccgcttgtca tatacttgaa
     1741 taatttgtaa ttgacgcctt gtgctttggc ttgtttttct gttaaaccta cattggataa
     1801 agtcggagtg ataaatacac tgtaaggcac agctttccgg ttattcgttg ttcgtttatt
     1861 ctcgcccttc aactgatcca taatgattcg ataatcatct agagagatat aagtgaactg
     1921 caatccgcct ttgacgtctc caatcgccca aatgttgtct gcatctgtac gaaggtattc
     1981 atccacagcg attgctccgc gatctgttag tttaacatct gtattttcta atccaagtga
     2041 tgctgtactt ggttttcttc cagttgcagc caaaactttt gacgcttgga ttgttacttc
     2101 ttggccatct tctgtaaagg tgacggcagc tgttgattct tggtctgcta cttgatctac
     2161 agatacacct agatggaatt cgattcccgc atttgtcatg tcttctaaaa tcatttgcgc
     2221 gatatcttca tcttcacgtg gaagaaattc agaatgggca tcaagaacga caactttaga
     2281 tccgtattca ttgaacattg atgcaaattc caagccaata tatccagccc cgatgatcac
     2341 taatttttcc ggtaattgtt cctgatccat cgcttgcgta gaatcaatca agtagcgact
     2401 tgtattcaat cctttgattg gtaaaatgat tggttcagaa ccagtattga tgaaaatccg
     2461 ttcgccttca acaatcactt tttcaccatt attcgtcact tcgatttgat ggttagaaag
     2521 aaaacgtgct gtaccgtcca tcactgtccc agttgcctca tcgctgatca tatgataatt
     2581 tttggcgcga agcatgcccg tcaatttttc tttccgagca acagcctctg taaagtcgat
     2641 tcctctttct ccattgaaaa tcaatgattt agaaggaata cagccgatgt tgatacatgt
     2701 tccgccatac atacgtgttg acttttcaac gactaaaacc gattcacctt ttgaagctaa
     2761 cgccttcgct aaagtcttac cgccctttcc aaaaccgatc acaatatttt gatatttttc
     2821 catcattttt ccctcgcttc ttttttacct accgattggt acaaaaatat gataaccgat
     2881 aattgtctat tctccaaatc atctgcttta tttgtcaaaa aaaaccagac aaaaagcggt
     2941 cctttcacgt ttttagtctg gttcgatttc ctttattctt ctaaacaatc gttacttcta
     3001 catcgatatt tcctctagtt gctttggagt aagggcagaa ttcatgcgca tcttctgcaa
     3061 ttttttgggc ttcttcttcg ctgatgccat cgatatgcac ttctaatact acaccgatct
     3121 gatagccgcc atcatctaaa ttataaagag agacacgtgc ttttactgtg gactctgctg
     3181 tcattcctgc ttctttgatc atatgttgta ccgccccatt gaaacaagaa gcataacctg
     3241 ctgcaaataa ttgttctgga tttgttcctt ctgcatggat tcctggagga gtgattttca
     3301 tatgcatcga gccatttggt gcctcgactt ctccattgcg cccgccatgg ttgatcattg
     3361 ctgtttcgta tgcttttttc attgtactgc tcctttcgat tgaccatttg ctacttggct
     3421 ataattatag tgtatgacaa gtaaggcgct ttctacaaaa attctgctta ttcgaatacc
     3481 agaatgccgt cttctactat ttttccaaaa aacaaaaaaa tactactcgt gaacaacagc
     3541 gtcatatttg ttccaaaata aatagcaatc ttttctacat aatcttttat atgattgatt
     3601 ttaagataca tatataccag aaagttgacc ccataaacca atactgttaa aaaaataacc
     3661 atattcgtta ccattttttc ctccttttgc tggtctccct accagcactc gatttaacca
     3721 taccactttt tttgccgtcc aagcaactag cctttcaaaa agactggttt attctgctgt
     3781 taatgattca agatcagaaa aatgtccgaa taattttgct aattttacta acgccaactt
     3841 gttctttccg aattcatcat tttctatctg taatactagt aacggctcat gtaagcttaa
     3901 acggatcaaa aaccagccgt ttccgtatgg ctcttctaga tcatatcgaa taccttcttc
     3961 attttccggg tcgaagttca tccctttttc ttcttccaac agtcgcttca tttcttctaa
     4021 tatcttgttt ccttgttcgc gtggttcatc gctgaacaat tttaatcgga tttcttgtac
     4081 ttctacgggc tgtttcaact ctttgatttg ttcatctaat gtgatacctt ttctttttaa
     4141 ttctggcaaa agcatcagga tctttgcgat gacatatgca ccatcatcca gaaaatagtt
     4201 ttccttgaat gcagcgtgtc cgcttgtttc gatggctagc tgtgtatctt tcccaagacg
     4261 gttaagttct aatgctttat tgattacatt tcgatagccg gaaatataac gaacttggat
     4321 gcctcctttt tcttcaatga atctttgtag atgagaagaa gtaggggaat tcgtcacgat
     4381 tcttgttcct ggatgctctt ttaatacgat tgtacttaat actgcgatca aattatttcg
     4441 attgatcact gttccagaag cactgacaac tgcagaacga tccacatctg tatcgaaaat
     4501 gatcccaaga tctgcttcat ttgctaaaac agcctgcttg atactttcca tcgcttcttt
     4561 attatcagga tttgggatat gattaggaaa tcggccatct ggttccaaaa attgggaacc
     4621 tgttgaatcc gcacctaagc tattcaatac tttttcagca aagaatccac cagcaccatt
     4681 tccggcatca acaatgattt tccaaccatt tagtggtttg ctttctagtg ttccaatccc
     4741 ctttttaatt tttgtgacta aatcttgcgc ataaggtgtc aaaaggtctg ctttttctac
     4801 gcttccagtt tgttcgcttt ttattttttg ctgctgtgat aggataaaag caatatcttc
     4861 tttttctgct cctccttctg aggtaaagaa ttttattcca ttataataat agggaagatg
     4921 gctagcagtc agcataaccg cagcatcgca cgagaactgt tcaaattgag tggacataaa
     4981 catagcaggc gtagtagcta attcgaaatc gatgacatgt atcccttgtt ctttcagact
     5041 ttggatcaac gcctctttca gtgctggtcc gcttatccta ctgtcatgac caatcccaat
     5101 cgtccatttt ttatttggat cggttgattt cttctttttc tttatccagt ttgctagtcc
     5161 ccagccgatc ttttttacca tgtctgtcgt caagtttact tcatatcctt caactggtaa
     5221 tgcgattcct cgaatatccg atccattttg cagcgctttc atatctttca tataaaagtc
     5281 ctcctcattt attccctcat tttttccagc ttttctgcaa atcgctgtgc ttcttcttct
     5341 gagcggcaaa taataatgaa tgtatctaca ccggccatcg ttccgacaac ttcagggtaa
     5401 gccacttcat ctagccaatt cgttaccaca tcggcaccat ttcgttcagt caatacgata
     5461 accatgaact gaacagactg gatacgcaaa acctcgcgtc taattgctgt acgcaaacgt
     5521 tcttcattta aagaaacaga tggctggtta aataatgtat agcgtactat tttgttttca
     5581 ttttgacttt ttactaactt catttctcga atgtctcgag aaatcgttgc ttgagtcaca
     5641 tcggcgcctg cttcatgcag ccgcgcaagc agctcttcct gtgtcccaat aggttgttct
     5701 gtaatgatct ggcggatcag tgcttgtcgc tgtttctttt tcatattaga cacctctcaa
     5761 tgttgattat agcaaaagat aagtaatatt tcttcatatt ttggtatttt ttacttttat
     5821 tttttgtttt cattcaggtt gctaaccttt ttctatttta acacgcaaaa tcgtcttcat
     5881 tttttcaggt gctgattttc ttggatcgct gatgtaaatc tctttgtgtt ctttagaagc
     5941 tctttcatag ccttcttttg cgatgaatgc ttctaatttc tcaaacgttg ctggttcttc
     6001 ggcatagggc ccgacgtgta agatttgggc acaaagacct tcttccatct ttttgaatac
     6061 tacttgttct ttcaagtctt ccggaagttt gctagaacgc tgaatggctt cttgtgctat
     6121 cgcttctgta acaaagtcag gctgtcggat catcaattga taagaaaaat gttctttttt
     6181 catcactggt tcatgcaaat acttttcttg cagtccccat tgtccttcca gtggatagac
     6241 agtatacatc tgataatcag gaatcagcca atttttttta ggcgacatgc gtattgcata
     6301 gctgactgca tatagacagg ctactcttcg tttaaagtct tcctgattcg ggtcgccgat
     6361 ccctctgatg ctaaagaatg attttttagg aacctctagt atttgcgggc tttttttcat
     6421 actatagctc ttgtctttac gccattcaat tttttccatt tttcttcctc cgcgaaattc
     6481 catttagtta tttattatca tacctctttt tgagtgactt ggctaatcgt gttgacacta
     6541 tttttcagtt tgtgctagaa taagtgaagt aaataaatcg tacagataac acgtttcggc
     6601 gtgtcgagag agaggcggtt tctattttta taatagaaat cactctcttt ttttacgaaa
     6661 ttttttagag gtgatgtaaa tgacaaaaca aaaacagctg cttttagtac ctgcagtaat
     6721 tgcaacaggg atgatgtctt ttgcgggcgt attgatcgag acggccatga atgtaacttt
     6781 cccaactctt atcagacagt ttgatttaac gactgcacaa gtccaatggg taacaacgat
     6841 ttatcttttg atgatttcga tcattgttcc gctgtctact tatttgaatc gcaattttag
     6901 tctcaaaaaa ctctttttag cttcaaatct gcttttcttg gcaggtataa tgatcgattt
     6961 cttctctcct accttcatgg tcctattgtt tggtcgattg ctccaaggtg cagcgacagg
     7021 tatcgctctt ccgttgatgt tccatattat cttgacattt gcccccctca atcgacgagg
     7081 cgcgatgatg ggtgtcggta ctttgacaac ggccattgct ccagcaattg gccccactta
     7141 tggaggcatt atgacttcac attttacatg gaatcatatc ttcctgtttc ttgtgcccgt
     7201 tttgtttctt tctttgatta tcggctggtt tgctattccc gagatggcag tacaaaaaag
     7261 tggttatttg gattggataa gcgttttagg aatttgtctt ttgtttagtg gaagtctaac
     7321 atttttgagt acgttgggaa cagtttccag ctggattgct ttaatcatcg gcggtattgg
     7381 ctggttcctt ttttactatc gaagtaaaaa agtggagcat ccactgatcc gactgtctat
     7441 tctttcaaat aaaacattcc gcttgttttt gattggcttc ttagtttatc agtttttatt
     7501 acttggtgta tcttttgtct tgccgaattt ccttcagatc gttcaaggaa gtagtccttt
     7561 tgtcgctgga ttagcgatgc ttcccggcgc agcggttggt gctttgcttt ctcctttttc
     7621 cgggaaaatg ctggatcaat atggtccgaa gaagccaatt tttgctgggc tgattctttc
     7681 tttgattggt tggctagcat tattcctact cataggacac gcttcagtgg gtgtattgat
     7741 tgcttgtcat gtgttttata tgatcggtat cggattatct tacagcaata tgatgaccac
     7801 cggaatgaat gcactgaatg aagaactaca aggagacggg aacgcaatgt tcaatacctt
     7861 gcagcagttt tctggcgctg tggctacttc attagtagcg gtcattatca attacgtcca
     7921 gcatcatact tcccataact atgaagtgtc tactacatta ggatcaaaag cagcattagg
     7981 cgtcttgttg ctgttgcttc tcgtaagctt tgctcgattc gcctattatc tgttctttgc
     8041 taaaaaagcg tagattccat atacggcagg tgttgcatct gccgtttttt tgtgtgaaaa
     8101 tggttgtttg taaaaggagg cttttttatg gaaaaaattg ccattatcgg tggcgggatc
     8161 attggtatga ccctagccag ttacttagat acatcaaaat tcaatgttgc tttatttgat
     8221 gaagggacag gacaggcaac taaagctagc gctgggatta tctccccttg gttgtcgaaa
     8281 cgcagaaaca aacaatggta tcgtctggct aaagacggcg ctgctttttt ccctaaactt
     8341 ataaaagatt tccaacttga tcaaacgatt tacgcacaat cagggacact tttgcttcga
     8401 cctgaaaaag atttagctga attggctgtt cttgccgaga aacgaaaaga agaagcgcca
     8461 gaaattggaa acatcgaatg gctttcacct gaacagacat ctcaagcact tccgttattg
     8521 aaaccagcag tttcactgcg tatcagtggt ggtggtcgtc tggatggaaa agcatattta
     8581 gaacatcttg gtcagcgttt agcacaaaaa caaatggagc agatccaggc aaaagtcact
     8641 ttgcataaga agagcgattc ttggctcatc aagggaccgt ttggagaata cacagctgat
     8701 cgtgtaattt tgacacctgg cccggcactt aagcctttat tacaagaatt aggctatgct
     8761 gcagatatac gtccgcaaaa agggcagctc ttagcatttc agacatctta taagatgagt
     8821 caagactggc ctgttgctat gcttgacgga gaagctgatt tcattccttt catggatgga
     8881 aagatccttt taggtgctac ccatgaaaac gatcaaggct gggatttggc tcctactagc
     8941 gaagcttata agggattgac taagagtaca gaacagttct tgactgatcc agatcagata
     9001 aaaaactggc catacgagtt ccgtgtaggt acccgtgctt atacgtcaga tttttcccca
     9061 tttttcggcg tactgccaga tgatccaagc ttactggctg ctagcggtct aggctcttct
     9121 ggcttgacca caggtcctta tatcggctat ctattagcca acttcttaaa tactggtcat
     9181 tggcctacgg aagattatca aaaggacatc tccacctata ttacgaaaaa tccatcatag
     9241 aaaaacagct ttttcaagta aggatcccga gcgatattgc gcatggatta cttcttgaaa
     9301 aagctgttat tttagttcaa tttctgaatc aaattctcca aactttcttt tgcttcatct
     9361 ggcaagagat cttttcctat ctgagcttca atgaatcgta aacgaatatc aagtcgttgt
     9421 ttcaccagat ttttatagga cgtatcctca aaagaaacag gaaaatctgg tataggtaaa
     9481 tagtggatgt cttcaaatgg accaaacgac ttgaattctg ccgtttgttg cacgattctt
     9541 tcaacgattg acaatgtcgt ttcaggtttg atttttttat catcgaaata tccagtattc
     9601 aggacatagc aggctgtttg ctgtttcaaa aacaactctc gaaaagcttg atagtcttcc
     9661 ctaagaggat agacacggaa aggattagca aatggttcaa tgactagctc atctagctgt
     9721 ctttttgtag tgatattttc cgcgtctgat cgttttgttg ctaatgttgc accaaaagac
     9781 gctgctaaaa ctgggtcatt gatcttgata attggcggaa gcgaatcatc tttcatgatc
     9841 caaaaaacag catcgatctt ttcgctaaga tgatcgactc ggtcgggtgt cgcataacgt
     9901 gatttgactg tccgcccatt tccgtttcgt atatctcctg ttactaggac tttttctcct
     9961 ttacttgtac atgtcacgcc cacattctga caagtaagga aagaagaaat ggccggatca
    10021 tcagacggat agtcttgtgt tttatcaaag taagaaggtt ccaaggcagt agcaaaacca
    10081 ttttttcgag aaataacgaa agcatcgtca tgcaagatcg aaacagagtg accttccgta
    10141 tgttttgcta aggtaatcgt tgattttcca gaaccggaca acccaaatac agccatcgta
    10201 taggtctggt cattactagc atattgtttc atcccaccgt ggcaggcaat aaagccattg
    10261 cgatttccca atgcccaagc aagcgtcaaa gtcgcttttt ttaattctcc aaagtaacgt
    10321 aatcctagca cagccgctgt attatggaga gcatcaaaca aaatgagtcc atttggaaat
    10381 gtttcatgtg tccattcggg atcacaaaag atataaagat ctccctcatc aaaggaaaca
    10441 gacttttgat aagtctctaa gatcttcgaa ttcgctggct gaaaattcag caaataagaa
    10501 tacaaattgt tctcaaatcc ttctggaatc agcaaatgac tttgcacaga aaattcttca
    10561 gataggccaa cgattacttt tgtatggtaa aactggcgct tgcgactttg atacacagct
    10621 tctcttaaaa ttttctcgta tttgctttgt tccttgtcta agtcctcaaa gatccgccga
    10681 gcacttgctg ttcttcccac aatgcttcca tcattgaata ctagtgtcct aacatcagct
    10741 ggtaaatcta gttcttcagc atgtgagatt gttaaatctg tgatgatcgt tcctggactc
    10801 ccttttgcta gttgataagc tgtcttccta tcaaaaactt catgaacatt attaccgtaa
    10861 aatgtcgttt ctacaatcga tcgaatactg gataacagtg gatttccact tgtgatttcg
    10921 tttaattcga gactctcttt tgtactcatg gctcctcctc attccctaaa caaaaaaacg
    10981 ttttctttat tatacattat tcgagttttt atttgtactg ctgcgctgtg ggaagcaaac
    11041 gagttatttt tcacacaaaa aaaccgagaa cttgaataaa caagtttctc ggtttcatca
    11101 ttttcctttt atcggcttag taccagccgt ttgctaacca gaatgtttta gcagcatccc
    11161 atgaaccata acgagatgtt acatattgtt ctgctacacg ttcttgattg gcagctgaat
    11221 agtcgccgtt taagtatgaa gcatctaatt gataacggcc gatgtaacgt ccgttagtag
    11281 ctgtgtatga accgcctgat tctttttgtg cgatccattc ttttgcagag cttgaagttg
    11341 gcgctgttgc agcagctggg gcagcaggag ctacttcaac tgtttcaaca ggtgcaacgt
    11401 atgtatgttc ttgcgttgct gtttcagttg tagatgctgt attttctgtt gttttgtttt
    11461 ctgttgttgc tgaagaatca tttgaagttg ggattgttaa ttgttcacca acataaatgc
    11521 ggttgatgtt tgcgatcgaa tttttttcag caattgcatc gatcattgag ttgtctcctg
    11581 caaatttttg tgagatctta gataatgaat cacctgattt tactgtataa acttcatcag
    11641 cgtgagcatt tgtagttcct acaaacattg cagtagcacc agcagcaaaa gttgtaccta
    11701 aaacaatttt cttaaatgaa ttcatattaa actccttttt ttctccgttt tttattcttt
    11761 cgacgctatt aactttatca tttagaaata tttcataggt aaaagaaaca tgacatttaa
    11821 tgacaaaata acgaattatt taatatttgt aacatttaaa caaatatttt tttgatacaa
    11881 aaccataaaa gccttgttgt ataagcaaag taatcgtttt tctatgtttt tttatttttg
    11941 aatgaaaaac gcagattctg taacattcac tgcaataatc cataaaaaaa agcaaaaaaa
    12001 tttttgattt ttatcaaatt ctcttaaaaa aatgtaaaaa atacaaaaaa accagtaatt
    12061 gttacaacaa ttactggaca aaaactttga ataatgttaa ctattttttt attttaactt
    12121 tctccgcaat aatttctggg catgttccaa ttcgtttata acgacttctt ctgtttcttt
    12181 agctaatatt tcatctaata catcaagcca tttttccggt tcttttgttc ctagacgtcc
    12241 aatagcccaa gctgctgttc ctctgatgac agggcggaca tctgttaaac attctgaaat
    12301 ctcaggcaat gccgttcgat cacctagatt agctaaagca atcaatgcgt ttctctgcaa
    12361 aggtttcttc ccacgccatg aaccagctaa atgcccaaat tgctgtttga agtctttatt
    12421 agacatcgaa agcattggct ttaattttgg atatatttcg tctacttttg gttccatctc
    12481 ttcatgaaaa tgaaaatctt ttccttgatt gtaagggcag actaactggc agatatcaca
    12541 tccataaatg acatttcgca tttttttacg atattcttcc ggcatcatcc cttttgtctg
    12601 agtttgataa gacaaacatc gctgggcatt cattcttccg tcaccaagca atgcttttgt
    12661 cggacaagca gtcacgcagc gcatacagtc accgcaacca aattctcctg gtttatcagg
    12721 ctcaaataca atattagtaa cgatctcacc aagatagaca aatgaaccga actcttcagt
    12781 gatcaacaat ccgtttcgtc caataaatcc taaaccagct cgctgtgcta cagcgacatc
    12841 gatcagttct ccagtatcta cttgcggagc tagacgccat tcctcttctt tttgccattt
    12901 ttctgctctt gtttggatga attcgatcaa tcggttcaaa cggtctctga gtatatcatg
    12961 gtaatcgatc ccccaagaag cacgagcaaa ctgcccgcgt ttttcatccc taggcacttc
    13021 ttcatggatc ctagtaggat acgcaagtgc aatggaaatg atcgatttcg gctgatcaaa
    13081 tgtcatttct ggataaagac gctcatcgat atttggatgt tcaaagccag aagtatggcc
    13141 agctgctttt tgttcttcca acgattcttt taatgaatca aaaggttccg ccgtggtgaa
    13201 cccgattttg tcaattccta atcgtttgct ttcttgtatg atttcttctt ttaatgtcat
    13261 ggtcttcctc gcttgctgtt aacggctgta taacccataa tatagttttt cttttttcag
    13321 ctggacaaac tggatctgct gatcggcaat cttcttttct tccatccatt tttttcttgc
    13381 agcaaatact tcccaataat ttgttgaaat taattttctt ttgttatcta atgcattaaa
    13441 aaaattcaat gaaactggtt ttgtacttaa cagttcatgg agccttacct cttcccatgg
    13501 cgaaaactca gacagatcgc gggaatacaa tacttcttca tagagatttt tttgctgtgt
    13561 gtacgtgaat gtttgcggga ttttcccagg cctagcaatt tctactagac gattataaaa
    13621 catattcatt aatttgctgt tggcaataaa ttgactttta taataggccg actggatctt
    13681 tttgattact ccttctgctt tgatgactga aaataatgca ggccatgctt tgacggttat
    13741 ttcgtcgttt tgtttaacta atacgccagg tgtgaagccg actggagaaa ggaacacaaa
    13801 ataaccgata ccggtatttt catctaaagg ctgaaattca tcaaacaacc atttgcctat
    13861 gtacagctct tcatctggtt ctgttgtctc attttgattt gctatgaata atttagctaa
    13921 ttctgcttca agaaattgaa ggtcttgatc cttgtcggta ttttcctgct ggctgatcaa
    13981 tcgaaagact tcttctttaa tcgtatattt atctttacgt gtaaaattca tagccacacc
    14041 ttccttcctt actctaacta taacggaatt tcttcaaaaa aggtatctgt acgacgaaaa
    14101 acagcataat tttttttcat atctcttata aaaaatgcaa ggtctgctcc tcaagctaga
    14161 ccttgcactt ttcagctttc ttttgtttgt gacaaacctt catggatcgt ttcaatattc
    14221 catttcatca tctgatagta agaatctcct tgttctcctt cttttgctat ggaatcagtg
    14281 aacagtttat catagatttt caaacccgtt tcttttgcta cccgttccat gcttcttgag
    14341 tctacactgg tttccacgaa taaaaccggt acttcttttg ctcttatctg atcaatgatc
    14401 gctttcattt gatcaggcgt accttgactt tctgtattga tctcccatat ataagctgct
    14461 ggcaaatcat atgctcgtga aaaatacttg aatgcccctt cacttgttac taataatttt
    14521 ttgttctctt cgatagaagc aaaactttcc ttcgcttgtg tatctaattc ttttagtttt
    14581 tccacatatg cttctgcatt ttttttatag agtgtcgcat tttctgcatc tttttcagag
    14641 aatatacgtg cgatttcctc cacatatttt attccgttag ataggtctag ccaagcatgg
    14701 ggatctgctt ttgtatgttc ttcaccgcta gttaaataaa gaggttctac atttttgcta
    14761 actgcaaaat aatcttttcc ttcttctttt ttagccgttt ccatcaagtt atcgaaccag
    14821 ctgttacctg tttcaagatt caaaccgttg tataaaataa catctgcatc gcttgccttt
    14881 ttgatgtctt ctggtagtac ttcatattca tgcggatctg ttccgactgg tacgatactg
    14941 tggatatcta tttgatctgt tccgacctct tttgccatgt ccgccaagat cgaattggta
    15001 gccacgactg acaacttttc tttacttcct tcatccgctt ggtcagaagc ctgctttccg
    15061 caaccggcta ataccaagaa actaactgcc aagattaaaa agagtgattt ttttgttttc
    15121 atctatctgc ttcctttctg aaataaaatt ccttttgttg gagaaaataa aaaagcaagg
    15181 ataaaaaatg cggtacatgc taacacgatc gtagctcctg atggccaatt gtaactataa
    15241 ctgaaaaaca caccgacaac tgcacttagc ataccgattc ctgcagctgt aatgatcatt
    15301 ttcagtaaat ggttcgttag caagtaagcc gtggcggctg gtgtgatcaa catggcaatc
    15361 accaagattg ttcctactgt ctgtaaactg actacagcca ctaatgtcaa aaagaacatc
    15421 aaaagataat ggatcagcga tgtgttcagc ccataagctt ttgccattgt cggatcgaat
    15481 gatgtgatct tcaattcttt ataaaagaaa aagacaaata tcaagacgat tccactaacg
    15541 agtgctgtca gcaacaaatc gctttcccta acagctaaga cattcccaaa taaaatatga
    15601 tacaaatctg tcgcactctg ggcatatgaa atcaagataa tccctaatgc aaaaaaggag
    15661 ctaaagacaa ttccaatcgc tgtatcattt ttcaatcggc tccgctgagt cacgaaacca
    15721 atcactgctg ctgttagcat cccaaaagca gtggctccaa agatgtaact aaaaccaaac
    15781 atataagaaa tggctacacc tggaagaact gcgtgagaga tagcgtctcc catcaaagac
    15841 atccctcgta aaataataaa agaaccgatg actccagaag caagtcctac aagcatggaa
    15901 gtgaccaaag cgtattgcag gaattgataa ttcattaatc catcaataaa ggattggatc
    15961 attttttctc tcctcctttt actaaatgtc tgatgatctc accataggcc aattgaaggt
    16021 tcttcgatgt aaagacttct tgtactggac cggaagcgat cagctgttta tttaaaagaa
    16081 taacctcatc gaaatattct tctactttat gaagatcatg atggacgatc aaaatcgttt
    16141 ttcctgaatt cttcaattcc tgcaagatgt caaagatctt tcgttcactt aacgcatcaa
    16201 tccctacgaa tggttcatct aaaaagatcc attctgctcc ttgggctaga gctcttgcaa
    16261 taaaaactct ctgaagctgt ccacccgata gttcgctgat ctgtcttttg gcatattctt
    16321 ccatccctac ttttttcaaa gcttgtcttg cacgttcttt ttcttttttc ccaggtcttt
    16381 gtccaattcg taaagatgga tatgttccta aaagtacaac gcctaataca tcaattggaa
    16441 aggaaagatc caattcactt cgctgttcca cgtaggctat ttttttacgg actgagttta
    16501 ccgactgtcc agaaaaggtg actttaccag tagtagctgg tatcaactct agcaaagatt
    16561 tcataaatgt tgattttcct gcaccgtttg gtccgataat accggtaatt ttttgtggat
    16621 ttatcgtcaa gttgatagtt ttcaaaacag ttttcccttg ataagcggct gaaaggtctt
    16681 gaattacaat ggccgtacgt tccaaatctt acactccttt ttaggtttac ctaacttttt
    16741 atttaaatat agtttaacta aaatggaaag cattttcaag tagaatgatg aaaaagtttt
    16801 tttcgaattt tcctcgcatt ttatgaaaat tttctgtaga atcacatcaa gagttcaata
    16861 aggctaacct aataatgaaa aacatgtgtt gaaacgtttg attatttttt cttgttgact
    16921 caacaacgaa aggagttttt gtatggaaga agaaaagtta actcgaagtc ttagtgcccg
    16981 tcatatccaa atgatcgcac taggtggaac gatcggtgtc ggtctgttta tgggtgcctc
    17041 ctcaaccata cgttggacag ggccctcagt gatgggagcc tatgcgattg caggattctt
    17101 tttgtatttg attatgcgtg ctttaggcga aatgctttac gttgatcctt cgactggttc
    17161 ttttgcaaat tacgcgagcg agtacatcca tcctgtggct ggctacttga ccgcttggag
    17221 taatattttt caatatattg tcgtaggaat aagtgaagtg attgctgtag gatcttatat
    17281 gaattattgg tggcctgatt tgccggcaat tattcccgga atcattgtcg tcttgtttct
    17341 tatgctggca aatcttattt ctgtcaaggc gtttggcgaa ttagaatttt ggttttcgat
    17401 catcaaagtc ataacgatca ttttgatgat cattgcaggt cttggggtaa tcttatttgg
    17461 atttggtaat catggtcagg cagtagggat ttcaaatctt tggaaaaatg gtggattctt
    17521 cactggaggc gtcaaaggat ttttctttgc attatcgatc gttgttgctt cctatcaagg
    17581 aatcgaactg atcggaatga ctgctggtga agcagagaat cctaaaaaga cgatcattga
    17641 agctgtccag tccactatcg gtcgaatctt gattttttat attggtgcta tttttatcat
    17701 tgtgagtatc tatccgtgga atcaattgag tgagattggt tcaccttttg tacaaacttt
    17761 ttctaaagtc ggcattacat ttgctgcagg actgattaat tttgtcgtga ttacagcggc
    17821 attatcaggt tgcaattcag ggattttcag cgcaagtcgt atggtctata cacttgccat
    17881 aaatggaaag atgtctaaaa aatttctgaa actatctcgt cacggtgcgc ctttttatcc
    17941 tgtcgtagca atctcaggtg gaatcttaat tggtctgatc ttaaattatc ttctaccaat
    18001 gttcatcaac cagtcagaaa atattttcgt tttcgtatac agctcaagta ttttgcctgg
    18061 tatggttcct tgggtagtga ttttgatcag cgagattcgc ttcaaaaaga agcacgcaga
    18121 taaaatgcat gatcatcctt ttaaaatgcc actatctcct tatacgaatt atgtgacact
    18181 tgctttttta gctatcgtat tgattttcat gtttatcaat cctgaaacga gaatctcatt
    18241 gattattgga ctcgttttct tgctttacat gacgatccat tactttgtac gcgaaaaaag
    18301 aaccggaaaa taaaaaaaga acgaagcttc accttagtgg aagcttcgtt cttttttatt
    18361 gcttttatca attcaatttt agcgtctcac cgggagtcag gaaataattc tccatgctca
    18421 tcccattcag cgacaagatc gtttctactg gtacgccagt tcgttcggct atttgatttg
    18481 gaccttctcc ttccaaaact acggttgtcg cagcctgtga agtcgcgctg ttggtattag
    18541 tttcagagga atctgttgtg tcttgtgttg ttcgagcttg catttcagaa gcttctaccg
    18601 actctgctgt ctcagaatca gaagatgttg tgtcttgtgt gcttgttgac tctgcttgaa
    18661 gagaggcgct agtctgcaaa taaatatcgg aaatcttagc gacatgattc gtataattag
    18721 ccgataattg attgatcaaa gtatctaagt ctacaccgtc tttattttct gctgcctgat
    18781 acgctgcacg taattttctg atcccttcgt cggagattgc ttgaagctca cgtgcttttt
    18841 ggttagcaac tgctgataaa ccagctattc cctcttgatt tccctgaatt tctgtctggt
    18901 attcttcaat caatgcaggc gtcttttctt cgatcttttt cgaataatca tttacgattg
    18961 cttgaagttg ctctggcaaa ccatttgctg ttttttcttc agaagtttgt gtctcttttg
    19021 ttttagttga tgactcactt gtttgtttcg tctctgtttt ctttgtcgtt gtttccgtta
    19081 tggtcgtact tttcgcttct gacgaatttt ttccatgaaa atatagaccc gcaactcctc
    19141 ctgctatgat caaaaacacg gcaactgctg ccccaatcat gatcatccgc ttacgagctg
    19201 cctgtttttg ctgtcttctt cgggtcatcc gaacattttc tccccactca ttttcatcat
    19261 acattttttt caccaccaat ttttatgtat aatagcgtta tcaaacgctt catattctta
    19321 ttgtagcaaa taattgtcgc tgtgcacatt tttatctttg ttatttataa gaaaatttca
    19381 ccagttgaga tgattccaag attgtccgtc gatttctcct tgcagctgca ttgttcgttc
    19441 gtctatctca gccatcagtt tctttatttc atataagagc gcacggtctt gatattctga
    19501 tttttcgctt aattcatcta attgttggat cagccaatca tttgtcataa tgaagttcct
    19561 ttcatttctt gcagtgtaaa ggtcaagtct tcgatctgct cttttgcctc agtcagtgct
    19621 tctacgatat atgcttgttt ttcagattct tcttcctgca ttttctgatt ttcctgtact
    19681 ctttgaataa accagtcaat ccgctgagtc agcgatggtt tgccttccaa tgagagatac
    19741 tgctgataag caattagctt ctcccaacgt ttttcctggg ataaatacat aaattgtgct
    19801 aaaacgaatg gcggaagata ggtcatatgg aatttatttg cgattcgctg atacggcctc
    19861 aaaaattcgg aaatcgtata ttcttctttt cctccagctt ggtattcttt ttccgccaca
    19921 cctgttgtca atacgattcc tagttctttc ccttttaata catgtgctgc tcgttccaag
    19981 acattatctt gccaaatttt taaatgagca ggagaactat accagtaaaa cggaaactgg
    20041 aaaatgatcc gatcatgctg ggccaagagc tgtagttcag tctctatatc tatttttccg
    20101 tctggataac ttgattccaa atgatgccaa gtagctggta aaccagcaga agcttctttg
    20161 aaaaattgct gatttaaaga ttcttcgatc gttggatggc taatgatgat aagtgtacgc
    20221 atcccttttc ctccttttta gtcttcgctt tacagtgtag cataaccgcc acaagtttct
    20281 tttgccgatg cttgtcattt taaccagact aatcaggttg tccaagaaac tgcggtttgt
    20341 ttttccaact gttttttcaa ggtatccaaa gcaatattct taaaattttc gtttttcata
    20401 cgtttacgat agttcgtagc cgtttctccg gtactctttt tgaacattcg gctaaatacg
    20461 actagactat tgaaccctac ttgataggca atactttcca cggattcatc cgtcagagaa
    20521 agaagaaacg cccctcgatt gatccgtacc ccgatcaaat attccttgat actggtaccc
    20581 atatgatttt tgaatactcg cgacaaatgg ctccgattga ccgctaaatg ctgggcgatt
    20641 tcctcaacag acaatttttc agcataatgt gcctcgatgt atttgactgt ttcaacagct
    20701 aaagaggaat gaaccttttg tacaccaccg ctgacctttc cgccatcttt taaaaggact
    20761 gataaaaaac ggtaggttaa tgctgttaac tgtaattcat tttccaaggt ttcttccgta
    20821 tctttcatgc attctaaaat aatgtcgata tatttttgta tgtcagaaga gaccatcgtg
    20881 tattggtctt ctctgaaaag cgactgccga atgatttctt ctgcagcact cccaccaaat
    20941 gaaagccagc aatacatcca cggatcttct ccatctgata agtaaaacgt cgagtctcct
    21001 ggacgaatca agaacagatc accttttttc aattggaatt gttgattttt cacatgataa
    21061 gatcctttgc cttccatcac gacatgcagg atgtaatcat tgcgaatagc aggaccaaaa
    21121 gaatgaaacg gcatcgtttt agagaaacca caaaaatcaa aatacaattc tttttctact
    21181 tgttgatgtt ccttttttaa aaaaatcgat tgctccacgt ctatccctcc ttactccttt
    21241 agtataatgc aacaaaaagt taaataaaag tcacagaaat atattaaaaa ggaagcgatt
    21301 tcttgttaag tttaagtcat taaaagatct ttgttattcg ttgtaaccgg tttacttagt
    21361 attatatatt gaacaggaga ggaatacaga ttatgaaaag caaaatgatc caacgtgtgt
    21421 cctacgcttt aggcgctttt ggacacgaca cattctatgt tacattaagc acttatttta
    21481 tggtattcgt gacaagccag atgttcgcag gaacagataa ggcaacgaat gcaaaaatga
    21541 ttgctacagt tacaagctta gttgtaggta tccgcttagt tgaaattatt tttgatcctt
    21601 tgattggtgg catcatcgat aatactcgta caaaatacgg taaattcaaa ccatggttag
    21661 ttatcggcgg cgttgttagt tccgtactct tggtagcaat ctttaccaat ttctttggct
    21721 tagctactca taatacgact ttatttatca tattatttgc aatcgttttc atcttattgg
    21781 atagttttta ttcatttaag gatatcgctt tttggtcaat gattccagct ttgagtacgg
    21841 aaacaaagga acgtgaaaaa tttgctacgt ttgctcgttt cggctcttct ctaggtgcaa
    21901 atggtactac tctagtcgtt gtccctatcg ttactttctt tacctatctt gttactcata
    21961 aacatattga aggcgcttca ggctggttct ggttcggtgt gattgctgct gttatttcag
    22021 gcggtactgc gattatcact gccttgggca caaaagaagt cgatactgcg atccgtcagc
    22081 aaaatgaaaa agctgggatc aaagatgtgt tcaaagcagt tgctcaaaat gatcagttga
    22141 tgtggcttgc ccttgcttat atcgcttatg cgattgccaa tgcagcaaca actgccgtat
    22201 tattctactt cttcaaatat gtacttggcc aaccaactga attttggatc gttggtctag
    22261 ttgctgcctt acttggttta gtagctgttc cactattccc aatgcttaca cgcgtcatct
    22321 ctcgtcgcta cgtttacctt ggagggatcg caatgatgat ttcttcttac tttttcttta
    22381 cgatcgcagg ttcaagtttg ccaatcgtta tcatcgggct agtattgttc tacttccctc
    22441 aacaattgat tttcttatcc gctttgatga cgatcacaga ctctgttgaa tatggtcaat
    22501 ggaaaaatgg tatccgtaac gaagctgtta ctctatcaat tcgcccttta ctagataaaa
    22561 tcgctggtgc tttgtctaac gggatcgtag ggtttgtcgc tatcgcttct gggatgactg
    22621 gtaatgctac tgctgctgat atcactgctc atggcgtaac gactttcaaa gcttatgcat
    22681 tctatgctcc tgctgctttg atgattttct ccgctattat ttttgcttgg aagattacat
    22741 tgacagagaa aaaacatgca caaatcgttg agaaattgga aaacaaattg gctccagctg
    22801 atacaatgga agattaactt cacgatgcag tagttccttc ccattaagaa aggatcttga
    22861 aaatgaagat tacagaaaaa gattttggcc aaggttatca cttgataacc ttggagaaca
    22921 aaaacaagct agcactttct atctctgatt taggtgctag aatcgtcagt ttaaaatcaa
    22981 atgatcgaga attagttttg ggatttgaca ccgctgaaga atatatcgaa aaagatccgt
    23041 atatcggtgc ttctatcggt cgcaccgcag ggagaatcga aaatggccgc ttttctctca
    23101 atggaaaaac ttaccaatta gccacagatc caaaaactgg acataatctg catggtggtg
    23161 ctcctggatt tgaattaaaa aaatggtctt atgtcatttt gaatggcgaa aatgaagctt
    23221 ctgtcatttt tactactaca agtcccgacg gagaacatgg ttttccaggt acaatggatg
    23281 tggaaatccg ctatactttg acaaaagata atatctggcg agtgacctct cgcggcacca
    23341 gtgaccaaga tactttattc aatccgacaa atcatgttta cttcaacttg acgggagatg
    23401 cttctcagtc aatcgatcaa catgagcttt ggctcaatag cgaagcttat gctcctttgc
    23461 gaacagatag tattcctata ggggtaaaag aaaatgctgc aggaagtgct ttcgattttc
    23521 aaatacctaa aaaattagcg tccgtttttg ctagtgattt ggatcaaaaa aatcttgttg
    23581 acggaatcga tcatccattt ttcttaaaag aaaccggctt gggtaaagaa gccgctcgct
    23641 tgactagccc cgataaaaga atccaagtag atattgctac agatgcttct agtgtggtta
    23701 ttttcactgc aaatttcggc acagaaacac cagaaatgcg caaccgaaaa ctggcacatc
    23761 acggtggcat cacatttgag actcaaacag ctccaggtgc agagcgcttc tcagcatttg
    23821 gttcgattca tttaaaagcc ggatcagtat ttgaaacagt aacagaattc aaaatcaaaa
    23881 caagaaagga ataactcatc atggaaatca aaagaagctt aaacacaaaa tttgccgaaa
    23941 cattcggacc taaaaaaact acacaatact tctcaccagg acgcatcaat ttgattggtg
    24001 aacacacaga ttacaatgga ggccatgtct tccccgcttc catcacttat ggaacgcaag
    24061 gtgtagcagc acctcgagac gataataaaa tattagttta ttccacaaac tttgacgata
    24121 tcggtgtcat tagttttact ttagatgagc tagaatatga taaaaaagca aactgggcaa
    24181 attacgtaaa aggaatggtc cttaaattaa aagaagctgg acactcaatc gatcatgggt
    24241 ttgagttatt agtggaagga acaatcccta atggtgcagg actttccagt agcgcttcat
    24301 tagaattatt agtaggtgtt gtattagaag atctgtttga tttaaaaaca gatcgcttgg
    24361 aactagtcca aacaggtaaa aaagtagaaa atgaattcat cggtgtcaat tcaggtatca
    24421 tggatcagtt tgccatcggc ttcggtgagc tagatcatgc aatcttatta gatacaaata
    24481 cgctgaaata cgaaatggtt ccagtgaaac tagatggtta tgcaatcgtc atcatgaata
    24541 cgaataagcg gcgggaattg gcagattcaa aatacaatga acgccgtagc gaatgcgaag
    24601 aagccttggc acgtttgcaa aaaactttgc ctatctcttc tttaggagac ttagatgaag
    24661 aagaatttga atcaactatt gaccaaatcg gcgatgatac attgatacgc agagctcgcc
    24721 atgccgtttc tgaaaatcag cggactttga aggcaaaagc tgaattagaa gctgggaact
    24781 tagaagcatt tggtcaactg ttaaatgatt ctcaccactc tcttcgctat gattatgaag
    24841 taactgggat cgaattagat acactagtag atgctgctca aaaacaagaa ggtgtcttag
    24901 gtgcacgaat gacaggagct ggatttggtg gctgtgcgat tgctcttgtc aaagaagaaa
    24961 atatacctgc cttcaaaaac aatgtctatg atgaatacat gaaagttgtc ggctatgctc
    25021 cggaatttta tgttgcacac attggaaatg ggacaacaaa gatcgatcca gaagctgttc
    25081 atgtttgata aaaaaatcaa cgatctcagc taaaaacaaa tagataaaaa taacctctca
    25141 gcaatttcca aatgtgagcc ttttgctcgt atttgaaaat tgctgttttt tattgacacg
    25201 gaaagagaac gtgtgttcta attgtgttga agaggtgatt gttatgtggg cgaatcaaaa
    25261 tccggtattt gtttatgaaa aagaaccttc aagagatatt ttatgtattg actgcaaatc
    25321 tttttacgcc tccgtagagt gcgttgaaaa ggatcttgat ccccttacaa ctaaattagt
    25381 agtcatgtcc tatccgtccg attcgacaga aacacggggt agcggattaa tcttggcttc
    25441 cagtcctgca gcaaaaaaag cttacggcat caccaatatc agccgtgcca gagatcttcc
    25501 cttcccctat ccaagtgatt tatatattgt tgccccaagg atggcctatt atatggaaaa
    25561 gaacagagaa atcaattcta tctataaaaa atatgcagac gaagcgaacc atcatgtcta
    25621 ttccgtcgat gaaagcttcc ttgatgtaac aaatagcatg gcactctttg gtaaaacaaa
    25681 tgcgtacgac atggctaaac tgatccataa agatgtgttt gagcagaccg gaatctacac
    25741 gactatagga attggcgata acccgctact tgctaaacta gccttagaca atggctcaaa
    25801 agacggaaag caaatgtttg ctgaatggcg ttatgaagat gtaccagaaa agttatggaa
    25861 aatctcagat atcactgact tttggggaat cggcaaacga acagcaaaac gtctgaaaca
    25921 aatgggtata cattctgttt atgatttagc tcacgcggat tattatcact tgaaaagcaa
    25981 gctaggggtc atcggtactc agctttatgc ccatgcttgg ggcatcgatc gaagtttttt
    26041 gggacaagaa tatacaccaa aatcaaaaag tatcggaaat agtcaaatcc tagatagaga
    26101 ttatacaaga cgagaagaaa tcgagatcgt tatcaaagaa atggccgacc aagtcggcac
    26161 tcgcttgaga cgatctgcag caaaaacaca agtagtcagc ttatgggtag gatactcttt
    26221 aggctatctt gatccaaatg gcaagaccgg gtttcaccag caaatgaaaa tcgaagcgac
    26281 aaatgctagc agagatcttg cagatgcttt attgaccatt ttccaccagc attaccacta
    26341 ccaggatatt cggcgtgtag gcgtgaactg ttccgacctc gtatacgcga caggtttaca
    26401 gcttaacctt tttgaagatc ctgaaaagca gataaatgaa gcgaaagttg attttgttgt
    26461 cgatacgatt agaaaaaaat acggctttca atcaatcatc catgcgcata gtctcttgca
    26521 aggaggacgc gccatcgcac gtagttctct cgttggcggg catgcaggtg gtatgagtgg
    26581 attagaaggt ggaaaagatg agacggacag caaaaaaatt cattgactat aacgagtatc
    26641 gagatcggcc atttggatta aaatggggga ctgcttttgc gatggaagaa ctagtcaacg
    26701 gtatcaaaaa aatgaagaag aagcacgcaa acagcctctt cctaaacaac aaatgacatt
    26761 gcaagaaatc gatagtatac tgacagaagc ctttttatct cgtaaagcag taatcattca
    26821 gctcaattta aaagatgaat ttggaagact cttggatgag gtcgaagggc acttcattgg
    26881 tgaagcctat gaagattact ttatgattga ccatcagccg atattgtggg aagacgtacg
    26941 gtatattgag ttaaaggaaa caaaaaaatg gtttgtgaca tgagaggttg tgacagaagt
    27001 gatcaacttc aaaaaataag aaaggatttt tcgaaaattg cttcttaaat ttttgtggaa
    27061 ttttggctta tttttcgaag aagcaacttc tggaacaacg tatattagat atcagaggtt
    27121 gtgaaaaagt cgcttagttt tgagtaacaa gccaaggcgc tgatttcaca acctctgtct
    27181 atctatccat ctattcggta agattcatgc atgacgtctt ctttatttag tatgtagagc
    27241 cctttttctt ctgaccattc aacataaatc agctgtttta catctgcata gccttctttc
    27301 ttcaagttct ccataagcca tgcttcagtc aattgattgg actgaagcac tttatgctga
    27361 atttgccctt catctacaag caggatcgat aaagctttgt cttcatctga ttttttcaat
    27421 acactcattt gaccggcatt ctctaggata acatgtttgg cattttcaag aaaaaagcat
    27481 tcttgctgtc ttaagattgc tcgcaactgc tccatctcga tatggttatt tgtcaactct
    27541 gtcatgttga tgacgccgtc tttgattaga acagatggct cgcctttgac ccaacgattg
    27601 accttttcat ttttttggac gatacgttcg ataccataaa tcattactgc ccaaagcgca
    27661 atggcaaata ggacatgccc tacatggaca ttgtcatcat aggttgattc ttctaagatc
    27721 ccacctaaaa caagcgtata gactaaatca aacggtgtga tttctgacat ggatttcttc
    27781 ccaagcaaac gtaccactaa taataaaccg atcaacccga acaccagttt cattgtaatt
    27841 gaaaaaatca ttttttctcc ccctaactat ctgttaatta aattatatat gctttctcac
    27901 gaacacgcat ggaatataca aggaagaaat gtatcctttt actaaaaaaa ggactgtgac
    27961 actgtagtga cccccaaaag ttagactttt ttggagtgga gaaatccact cctttttttt
    28021 atgctctaaa atgatatttt tacacaaccg ttacggttgt tttacgaaat tggacg
//
LOCUS       DAYYKN010000021        27884 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000021 DAYYKN010000000
VERSION     DAYYKN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.021"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACHBB7_000783"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACHBB7_000783"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM9208756.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="ACHBB7_000784"
     CDS             complement(937..1518)
                     /locus_tag="ACHBB7_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9208757.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACHBB7_000785"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACHBB7_000785"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM9208758.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="ACHBB7_000786"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="ACHBB7_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM9208759.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="ACHBB7_000787"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="ACHBB7_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM9208760.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="ACHBB7_000788"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="ACHBB7_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM9208761.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="ACHBB7_000789"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="ACHBB7_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM9208762.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACHBB7_000790"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACHBB7_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM9208763.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="ACHBB7_000791"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="ACHBB7_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM9208764.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="ACHBB7_000792"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="ACHBB7_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM9208765.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="ACHBB7_000793"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="ACHBB7_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM9208766.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="ACHBB7_000794"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="ACHBB7_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM9208767.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="ACHBB7_000795"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="ACHBB7_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM9208768.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="ACHBB7_000796"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="ACHBB7_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM9208769.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="ACHBB7_000797"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="ACHBB7_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM9208770.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="ACHBB7_000798"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="ACHBB7_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM9208771.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="ACHBB7_000799"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="ACHBB7_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM9208772.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACHBB7_000800"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACHBB7_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM9208773.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="ACHBB7_000801"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="ACHBB7_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM9208774.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="ACHBB7_000802"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="ACHBB7_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM9208775.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="ACHBB7_000803"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="ACHBB7_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM9208776.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="ACHBB7_000804"
     CDS             13972..14157
                     /locus_tag="ACHBB7_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM9208777.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="ACHBB7_000805"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="ACHBB7_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM9208778.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="ACHBB7_000806"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="ACHBB7_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM9208779.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="ACHBB7_000807"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="ACHBB7_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM9208780.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="ACHBB7_000808"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="ACHBB7_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM9208781.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="ACHBB7_000809"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="ACHBB7_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM9208782.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="ACHBB7_000810"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="ACHBB7_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM9208783.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="ACHBB7_000811"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="ACHBB7_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM9208784.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="ACHBB7_000812"
     CDS             18232..18879
                     /locus_tag="ACHBB7_000812"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM9208785.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="ACHBB7_000813"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="ACHBB7_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM9208786.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACHBB7_000814"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACHBB7_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM9208787.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="ACHBB7_000815"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="ACHBB7_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM9208788.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="ACHBB7_000816"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="ACHBB7_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM9208789.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="ACHBB7_000817"
     CDS             20317..21255
                     /locus_tag="ACHBB7_000817"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM9208790.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="ACHBB7_000818"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="ACHBB7_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM9208791.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="ACHBB7_000819"
     CDS             21935..22774
                     /locus_tag="ACHBB7_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9208792.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="ACHBB7_000820"
     CDS             22750..23619
                     /locus_tag="ACHBB7_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9208793.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="ACHBB7_000821"
     CDS             23609..24406
                     /locus_tag="ACHBB7_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM9208794.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="ACHBB7_000822"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="ACHBB7_000822"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM9208795.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            26019..26213
                     /locus_tag="ACHBB7_000823"
                     /pseudo
     CDS             26019..26213
                     /locus_tag="ACHBB7_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26401..27177)
                     /locus_tag="ACHBB7_000824"
     CDS             complement(26401..27177)
                     /locus_tag="ACHBB7_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9208796.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            27300..27707
                     /locus_tag="ACHBB7_000825"
     CDS             27300..27707
                     /locus_tag="ACHBB7_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFM9208797.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT         9090 a         5089 c         5865 g         7840 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctcgtca tatttttcgt ttggttgggc
    25561 ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaatcagcgt
    25621 tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt gccgtttgtt
    25681 gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg tgagcctgtc
    25741 atttaactct gagtcacaag taacaatttc aaaaaacagc ttctcatctt tgatgaatat
    25801 cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta ttttttctgt
    25861 aaccaaaaaa cgatggaacg ttgttttgat tgcccttaat gaggacagtc ggaacaggtt
    25921 tgtcattttt atatttaaat ggcacaaatt tttctaatta tttgtcattt agcgctgaaa
    25981 atttttgcta tttatctaaa aaagtgataa ctacttaaga tgaagcagaa tatttacgcc
    26041 atacggcata caaccgcgaa aaatataaaa aaaagaaaac gattgaacgc gtttttgcag
    26101 tcgcaaagga aaagcatgga atgcgctaca tcaagtatcg agacttggaa aacaatcagg
    26161 tgtatacgct gattgttttt gctgcaatga atctgaaaaa gttgtccact tggcagttgg
    26221 aacatatgtc ttcatatctt ttattataca aaaatagcaa aagcaccagt ctacactctt
    26281 agtaaagagg aaactgatcc ttttgtctac attcttaaag tacttaaact aagtacttta
    26341 ttttactctt taggattgat acagtagcat ctatcccttt attaataatc ttttattttt
    26401 ttaaataaag atgttgtctc catcttcagg cactaaaact ttttcggaaa ttcctttttc
    26461 tttattgaaa gactttaatt cttctcttga cagtccccaa tggtttacag cttccatatg
    26521 aacggcaata attgtggaat ctggtattgc tttatgcaat tcatataaat cgtctttccc
    26581 cataataaga gattctccaa ctaaaaaatg attatctcca gcatttgcta ctatgatttc
    26641 tggattatac tgatcaatag tttgtttaac agcttcatac caaactgtat ctcctgtaaa
    26701 gtatagtgtc ttctcagtcg aatgtttgaa aacaataccg caaacattac ctacataatt
    26761 caagatttct cctctgccat gtttgccttt tgttttaacc aattgtatag attcaaaaac
    26821 tgtgtcttct cgtagtacct ccacattttt aaaaccatat agtcgaattt cattcgcatc
    26881 attttcatct tgcacaaata attttatttc tttaggtagg acctctttcg ctatatcgtc
    26941 ccaatgatct agatgtaaat gtgttacaat aacagcatca ataccgttga taatatcttc
    27001 gactggcatt ggcaaatcaa ctagaggatt catttgatct tttcttggtg atggaaaagg
    27061 acctttcgaa ataaatggtg gaagtgttcc tttttccgct aacattggat caattaaaaa
    27121 ttttttacct ccatagtcta agatagctgt tgcattacga atgtgatgta tattcataaa
    27181 cgttgtctcc tttttgttga tatgaaatat ttttagtata attcaattcc atacaataaa
    27241 taaaccgaac aaaaaaagtc ttttactatt tttacttaat ttatgaaagg ttgtttacca
    27301 tgttagacca aacagatata aatatcattg aggaactatc aaaaaatagt cgaatcacaa
    27361 tgaaagaact tggagaaaaa gtacatttga ctggacccgc tgtctcggcg agagtgacaa
    27421 aactagagga aagtggagtt attgaaaatt atacagtaaa attaaatatg gaaaagctag
    27481 ggttttcgat tcacgctttt ttaattataa ttacgcaaaa tttaaatcat aaaccctatt
    27541 tatcatttat taaaaatcat gaaaaattct tgattagaaa ttataagatt agtggagggg
    27601 gctgttatct tttggaatgt agatttactt caaaaaaatc aatgaacagc tttttggaac
    27661 agcttaatga atatgcgaat tataaattat ctattgttat agattaataa taaaactagc
    27721 agatggtaaa aatactaata tgcgagacac acattcaacc ataactatat aacttatcaa
    27781 tttaaaacta aactgatgga aacaagtaag gcaaaaacat ttatattttg ccctacttat
    27841 ttttttaatt aaattcattg atgattcata tttagaaaat tcta
//
LOCUS       DAYYKN010000022        27838 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000022 DAYYKN010000000
VERSION     DAYYKN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.022"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..574)
                     /locus_tag="ACHBB7_000826"
     CDS             complement(29..574)
                     /locus_tag="ACHBB7_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9208798.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(586..1278)
                     /locus_tag="ACHBB7_000827"
     CDS             complement(586..1278)
                     /locus_tag="ACHBB7_000827"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM9208799.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACHBB7_000828"
     CDS             complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACHBB7_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM9208800.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1594..2157)
                     /locus_tag="ACHBB7_000829"
     CDS             complement(1594..2157)
                     /locus_tag="ACHBB7_000829"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9208801.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2385..3047
                     /locus_tag="ACHBB7_000830"
     CDS             2385..3047
                     /locus_tag="ACHBB7_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM9208802.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3171..4367
                     /locus_tag="ACHBB7_000831"
     CDS             3171..4367
                     /locus_tag="ACHBB7_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM9208803.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4468..5844)
                     /locus_tag="ACHBB7_000832"
     CDS             complement(4468..5844)
                     /locus_tag="ACHBB7_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9208804.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5881..6747)
                     /locus_tag="ACHBB7_000833"
     CDS             complement(5881..6747)
                     /locus_tag="ACHBB7_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM9208805.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6836..7861)
                     /locus_tag="ACHBB7_000834"
     CDS             complement(6836..7861)
                     /locus_tag="ACHBB7_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9208806.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7879..8553)
                     /locus_tag="ACHBB7_000835"
     CDS             complement(7879..8553)
                     /locus_tag="ACHBB7_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9208807.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8737..9630)
                     /locus_tag="ACHBB7_000836"
     CDS             complement(8737..9630)
                     /locus_tag="ACHBB7_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9208808.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9704..11401)
                     /locus_tag="ACHBB7_000837"
     CDS             complement(9704..11401)
                     /locus_tag="ACHBB7_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9208809.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11394..12707)
                     /locus_tag="ACHBB7_000838"
     CDS             complement(11394..12707)
                     /locus_tag="ACHBB7_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM9208810.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12707..16648)
                     /locus_tag="ACHBB7_000839"
     CDS             complement(12707..16648)
                     /locus_tag="ACHBB7_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9208811.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16757..17215)
                     /locus_tag="ACHBB7_000840"
     CDS             complement(16757..17215)
                     /locus_tag="ACHBB7_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9208812.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17513..18031)
                     /locus_tag="ACHBB7_000841"
     CDS             complement(17513..18031)
                     /locus_tag="ACHBB7_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM9208813.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18033..18650)
                     /locus_tag="ACHBB7_000842"
     CDS             complement(18033..18650)
                     /locus_tag="ACHBB7_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM9208814.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18647..19501)
                     /locus_tag="ACHBB7_000843"
     CDS             complement(18647..19501)
                     /locus_tag="ACHBB7_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9208815.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19498..20409)
                     /locus_tag="ACHBB7_000844"
     CDS             complement(19498..20409)
                     /locus_tag="ACHBB7_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9208816.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20450..21784)
                     /locus_tag="ACHBB7_000845"
     CDS             complement(20450..21784)
                     /locus_tag="ACHBB7_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9208817.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21992..22822
                     /locus_tag="ACHBB7_000846"
     CDS             21992..22822
                     /locus_tag="ACHBB7_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM9208818.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23066..23315)
                     /locus_tag="ACHBB7_000847"
                     /pseudo
     CDS             complement(23066..23315)
                     /locus_tag="ACHBB7_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23506..23700)
                     /locus_tag="ACHBB7_000848"
     CDS             complement(23506..23700)
                     /locus_tag="ACHBB7_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208819.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24205..24390)
                     /locus_tag="ACHBB7_000849"
     CDS             complement(24205..24390)
                     /locus_tag="ACHBB7_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208820.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25240..25581)
                     /locus_tag="ACHBB7_000850"
     CDS             complement(25240..25581)
                     /locus_tag="ACHBB7_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9208821.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVARYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25581..25811)
                     /locus_tag="ACHBB7_000851"
     CDS             complement(25581..25811)
                     /locus_tag="ACHBB7_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9208822.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26098..26436
                     /locus_tag="ACHBB7_000852"
     CDS             26098..26436
                     /locus_tag="ACHBB7_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208823.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26641..27203)
                     /locus_tag="ACHBB7_000853"
                     /pseudo
     CDS             complement(26641..27203)
                     /locus_tag="ACHBB7_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         8268 a         5948 c         4754 g         8868 t
ORIGIN      
        1 ttttatttta tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa
       61 aatgtctcaa tgcccattca tgtccaacgg gatcgaaact tgccacggaa tctttgaaaa
      121 tatggagttt gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg
      181 tacatacgcc ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat
      241 ctgttccact aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct
      301 catagtcttg gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca
      361 cattatgcgg aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat
      421 caatggcaaa aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct
      481 ttttttcaat tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat
      541 catatgtgta gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt
      601 gacaaattcg atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa
      661 gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat
      721 attgaactga cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc
      781 agggaaattc gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt
      841 aacgatcaat ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact
      901 gcgcaaatat gtgcttgctt cataatatag aggcattcct ggtaattgtc ctgggttgta
      961 gccaaatcct gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc
     1021 tacttgtaag ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc
     1081 gtattgttgg atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg
     1141 tacgacgatc acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc
     1201 ccatgataat ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc
     1261 gataataccg attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa
     1321 aaccacacat aacattagga taacaataat aagagatttc atcaaaaaag agttccattt
     1381 ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc
     1441 tttacgataa aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg
     1501 ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt
     1561 aataagcggt cctttggcca taagccgatc cctctacttt cctttggtca atagttccca
     1621 gtattgaatc gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg
     1681 cgcctcttca agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg
     1741 attctcgacc ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc
     1801 tggtgaaaga tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc
     1861 ttctaattct ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc
     1921 agctggaatc tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag
     1981 acgatcttgt tcgtcaaaag caatgatccc gacaccgcca ggatgaaata ccagttctct
     2041 cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc
     2101 gctatagatt tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc
     2161 ttctcctgct ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa
     2221 actccttatt agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc
     2281 tttagtccga tactctttct agtagtccgt atgctaaaat aagtatgtaa tcgaataacg
     2341 aaaagaattc aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt
     2401 ggccgtggct tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt
     2461 ttgttctagg acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc
     2521 gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact
     2581 atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa
     2641 caaaaaatga gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa
     2701 tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag
     2761 aaccaaaccg actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg
     2821 atttgacaaa taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg
     2881 aaaaactaga agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt
     2941 atcaaaaatt cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac
     3001 agagcttaaa gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat
     3061 aaaagaagac tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta
     3121 gagacattca tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata
     3181 aaccgactga aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca
     3241 acccgttttc aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc
     3301 tccaagagca acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac
     3361 aagctaaaca gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg
     3421 gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag
     3481 cacaagatat tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag
     3541 ccaatcctga cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg
     3601 tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca
     3661 agatttcagt gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag
     3721 aacagcttta ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg
     3781 gagaattgaa aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag
     3841 aagaaactgg cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc
     3901 gtctggataa acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac
     3961 cacaaatccg tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta
     4021 tcgctacagc cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc
     4081 aaaaagatgc agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga
     4141 aaaattcaga aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa
     4201 tcgtggatat cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga
     4261 cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac
     4321 acatggaaga agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct
     4381 tgttactaaa acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta
     4441 tctttcttct aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc
     4501 atagccaaaa taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac
     4561 agtttgcaga cgaagcatca atacaataca agtcaaaatg gagacgattg gcaatagcgg
     4621 aacgaatggc actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag
     4681 taagcctaga ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa
     4741 tacattcaat gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc
     4801 ttttttcggt gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc
     4861 cttgctgaca gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag
     4921 tagcgttaaa attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct
     4981 cattgcaaaa gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa
     5041 gatcagcgtc acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct
     5101 tggcacatct ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc
     5161 caagaaagca aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat
     5221 gaatggctgc cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat
     5281 gataccaaat ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag
     5341 cgacacgatg taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc
     5401 attttccgga ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa
     5461 aaaaccttgc atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc
     5521 cccgatcaac aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata
     5581 ggcatatggc ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga
     5641 caaaatgaca acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg
     5701 attggccgca acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag
     5761 catgatcaaa tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat
     5821 ctctttctta cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga
     5881 ttaaactgtt ttcatatacc ctaaaaggat accacctaaa atgactaaaa ggcaaccaac
     5941 aataacataa atgagctctt ttttcgtttt tctttcgcca aggatgaaaa tcccacctaa
     6001 agtagagata atgatcccca tttgtgaaag tgagaagcca acagctaaac caagtgattt
     6061 gactgttaga agcatgcaga tgtttcctaa gccccataat aatccacaga tcatgttttt
     6121 ccacacaaac tgatcgactt taactttacg gaaagcaaag aagcttgcgc ctaagatcat
     6181 tccgatactt tgaggtaaga taattgacat cgcatccaaa ttcgcccagt tgacgatgat
     6241 ggtatataca ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct taccaaaatc
     6301 aagcatttta ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag tcaaatacgc
     6361 accgcttaca agaagagcta aagcaataat accgtataca taatctttcg tttgtgtcca
     6421 ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac cagtagacaa
     6481 tggtagccct acagaaacac ccatgtactt cattccatgg aactgtccat tttgtccaac
     6541 actccaagct aagccggata aaaatccgat caagaaaatc caaggggtga ttacaggact
     6601 aacaacgaaa aagacaacta aagaaaacag aaatgcaccg atcgtcattc ctagtgtttg
     6661 ttgattggca tcgccaccaa ttttaccact tactaaacca atactacccc atgccaacat
     6721 agggactaat gctattaaaa tttccataac caatctcctt ttatttcaca tgataagaaa
     6781 cttatcagcg aaaacgttaa caacacaagc actttttttc gtttctaacg attttctaac
     6841 gtttttcatt ataaaaattt aagattggga aaaataaagt cattgttgtc ccttttcctt
     6901 cagttgattc agccgtcaaa tggataccta atttttcggc cagatttttt gctaaataaa
     6961 gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt tctcccagta aatcccttat
     7021 caaagatccg tttcaagtct tccgctggga taccgatccc tgaatcttta actgaaaggc
     7081 tgactccttg cgcttccttt tctatgctca caatgatgtt tccgtgatcc ggcgtatatt
     7141 tgatggcatt actaagcaat tggttgaaga taaaagcgat ccatttagca tcggtcaaaa
     7201 cagtatgatc ctcaccaacg atctcgtagc gaatattttt ttggataaaa taatttgcct
     7261 gactgcgtat cacaggctga atgattcctt ttagtccata ttcttggatg aggtagtccc
     7321 gcgagaaact gtcgagtctt gcataataca aaacttgttc tacgtattca tcgatttttg
     7381 ataattcatt ttctagtaat gtatactttt gatcgtcaat atcgaactcg atcgaacgaa
     7441 ctagcaattt agaagcggaa agtggcactt tgatttcatg gacccatgag tcaatataat
     7501 ctttttggtc ttgttgatta ctgattgctg attgcattac ttcttggtgt tcacggatca
     7561 gctgattgat atattcttct accagttttt cttcttctgt cctcccacca ctcaaataat
     7621 tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt ctcttgctca
     7681 agtaaccacc cagcagataa aaaatcagaa agacgccctc aatcaaagct aaataagcaa
     7741 ttgtgctcaa atcaactttg atgtttggcg ccagccacat cacaaagatc gtcaatgcaa
     7801 taaaaaacag ccatcccaag atcaatgtcc actgatcttt caaatattta aaaaaattca
     7861 tcttaaccgc tcatcctttc agggaacgat atatccctgt cctacttttg tttcgatata
     7921 tccttggata cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga ctgtcaatgt
     7981 attgtcatct acaaatcgtt cgtcatccca cagtgctcgc agcaattttt cccgacttaa
     8041 gatctttcca ttgtgcttca tcaaaataaa taatagacga tattcatttt tgctcaaatc
     8101 aatgatctct ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa tgccattgtg
     8161 gctcatcatt tcactagata attctgtgta gttgtaagaa cggcgaagta atgcattgat
     8221 ttttgcaatc agtacatcga tttggaaatg ttttgtcaca aaatcatctg cccccatgtt
     8281 catagccatg atcatatcca tattcgtatt cctgctggag ataaaaatga tcggtacttt
     8341 cgatacttca cgtatttttt gacaccaata atagccatcc aaaacgggaa gattaatatc
     8401 tagtaataca agctgtggtt cttctctttc aaagtctgag aacacttgat tgaaatcggt
     8461 cgttccaaag gtttcaaatt gccatttctc aagttcctcc atgatcaatt gtcgaatgat
     8521 ctcttcgtct tcgactacca tgatttttgc cataaaaaat ccctccatct ttcttcttgc
     8581 tactacaata tcaaagaagc ccttgtgaaa caagttggct tacctggaaa atgtcatttt
     8641 agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc ttccaagcag
     8701 cttaaattta ttatgttatt cttaataatt ggtctattat ctgttatttt tctttcgttg
     8761 tttttgtctt ttaaattttc gataacacca ataaccgaaa agcgttagca ctagcaatat
     8821 acctagaagg agtataagta atcgataaag atggtatttt tttgtctgtt ctatctttga
     8881 agagacttct tctgtaaagg gaactcgtct tcctgtcact agtaatcggt gagtgttcac
     8941 ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc ttttctctaa ttgctaatgt
     9001 atttgttttt gttggaagga caacaatttt ctcaacgact tcataagcga gcatccgacc
     9061 atacacttca agatagaatc ggtcatcttg ctccatttct tcaagatccg taaagagttt
     9121 tttctcagca ataccgctat gggcggtaat cacggaatgt gtactctctc ctccagttgg
     9181 aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac aaaagatcat tcgtttcatc
     9241 aaataaaggc aagctaacat tgattttcgg aatatagatg gctccgatca tatgttgttc
     9301 gtaatattct tttcctggat tttggacatt tctaaccgct tgctcaaaag gatctttgac
     9361 ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc tgatttttct tttcatgcac
     9421 tgctaattgt tgctcatgtt tttcttcttt atcgcgttca agctgctttt gataattctc
     9481 gatccttctt tgatccaaat aattgctgag agcatctgct aaaaatggat atgtaaaaac
     9541 tgcagctccg gcaagaaaca tcactgccat aaataattgt aataaaagac tgatttgggt
     9601 ttgaggtcta ttacctattt tctttttcat tttcttcacc ttcttattta aaaaaactgc
     9661 agcaaccttt ataaaaccga ttgctgcagc ttatttttgt tgcttatact tcagacttca
     9721 ttttagattt acggtaccag ctgtacgctc caatcatcaa acttattccg attagtaaga
     9781 atgccaagag cccgttccca ccagtagcag gaagtgaccc gcgtttcata ttcggtactc
     9841 tttctggccc tggattttct actcctacga tctgaatttc ttcattatag cttccataag
     9901 agatgataaa ttcagtaaag gctgcctcac ctgttggaag tacataacct tctggcgcca
     9961 tggtctcttt catttgatag gttccataat ctaacccttg aacctcaaag tatccatttc
    10021 catctgcgac aaattctgtt gcttgtccgc ttgttgccca ctcaattcta tctgctcctg
    10081 attcataaac agctaaaggc gactcccctt tataaaatat tgcgtattca gaacggtctt
    10141 cattccacaa ttcgaatctt gcatctttaa gcaattcact gcttgatgca tcatattttt
    10201 caaactttct tccacctgtt gtgactttct ctggcggctc tactccttct tcataatcaa
    10261 attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca tccgcttcta
    10321 gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt gcatctgcta
    10381 atctacctaa agtatctact ttggctactc cattttcttc tgtcaaaatc gtcacgacaa
    10441 acccgttgct tactacttct actatataat cttgtccttt tgttaaaatc gtctcgccgg
    10501 ctcgcacttc tatttcctga tcataataag ttagaccaac tgtaggcgta tctgtgattt
    10561 tgtaattttt gataacagcg cttccgtttt caaggacaga atcgatgaaa taaggaatag
    10621 gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag atcaatataa gaaatcgttt
    10681 cttgttctcc agcttcatta ataatcgtta cttgtctaag gtcgctttcc tctacattca
    10741 tctgtttatc tgcttctctg atttcgtttt tcggatacaa atgcacatct gtattgtatg
    10801 tgtctttcca tgtagaacca tcccacacgc catccaccat atcaggcatc atcactggca
    10861 tcataacaac cattggagac gcgattctgg aaatatgagc tggcgaataa gtttctaaga
    10921 aaagatagac tttatctcta gaagaaggtg cttcttccgt cacttggacg gtagagaata
    10981 ccgcttctcc agcttcattc gttgtttgtt tgtcaatact tgtcagtcga tattttaaaa
    11041 accaactttc tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa ttaagaccgt
    11101 cttctcgttc cggtgttaaa ggattatctc gaatcaaatc gtaatatact tccgttacgt
    11161 caaacaaatc aaaatcgact cctggaatat tttcttcacc aaaatccgta gaaaccaatc
    11221 cgctattcgg tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat gtaatcgtca
    11281 cttcacttgg tttttgtgcg acttcttcgg ctttgactag ttttccgttc ccgaaaatcc
    11341 ctattcccgc agtaacgagt aacagtacac tcattcccgt gacaatcatt tttttattca
    11401 tttttttctc cccgttcttt ttttattaat aaaacagcac ctaacaataa aagaattcct
    11461 agtaaagaaa tcaacgttct tgcttcattc gtttgcggga atacggacca ttttcctgat
    11521 tgacctggat tgcttggacc actcggctct ttcggtttgt ccgttgtttc ttttacggtc
    11581 tcgttataaa cacgtggtgt tgcggattca taggcagaga ctggaacttt cccggattct
    11641 aattcagcca tctcctgtcc gttaactact acatattgtg gttggccttt tgcatccacc
    11701 caaaattcag ggatgatgac ctcgattctc ttacttgctt catccatctc gtaaccttcc
    11761 acacctttta attcttcaaa ataataggtg ccagaaggca agaaacgttg tcctgtcgta
    11821 acaaggccct ctttatccga aatgaatttg cttacatttt tgtcattcaa aggatcattg
    11881 tctgctggtt tgatccattg atttttcaag tcattagcag gagccatatc taaataatat
    11941 ttcccccctt ctatcatctg atatagagca aaaacagcac cttctaatgg ttttcctttt
    12001 tcagttgttc ctctttgacg tccatacttg aagaagtatg gatctcttag ataaccgaca
    12061 tttttaggat agatatgaat ctcttgcagt tcctcggttg attctgtcgg gtgataaatt
    12121 ggcaaaatag cagcaattgg gatagctgtc tgatcgatat caacatccaa tccaacttct
    12181 ggatcaatcg attcttcgaa aatcaaataa actgcatccc gatcttggtt tttcttaggt
    12241 gctttgatac gtgcaatccc gtctcctaat ccgccaaccg tatctttccc agttgttacc
    12301 gaagtcccta tttggacaag cctttcttct gtgattagct gacggatatt gtttctgttc
    12361 gtttcagcga tttgctggac gaattcttta ggagtcatgt tttcagcagc ttttttttca
    12421 tgataataat cggtcatgtc gtaaatggca aatgtcgctc catttagtgg caccgtctgt
    12481 gaaatgatat ctgctgtttc ccctgcttcc tcagagatag ccaagccatc attttcatgg
    12541 tattcaaatt gatcgttata atcgatatct cgaatcatcc gtttatgcaa cacgaattcc
    12601 acgacatctt cccctgattc ttctgccgac atcgcatgtg gaagaaggac tgctgcaagc
    12661 ggtagtaaaa caagtaattg aagcatcaaa cacaacagct ttttcattac cttgctccct
    12721 cctttttaga ttgtctgaca aacaaaccat aggctgctcc tgcaagcgcc atgaagaatg
    12781 cagcaagcag gatatagaat aatcgtccgg atcctcctgt agcaggcaac ggattttcag
    12841 gatcgttccc gacactaagc tgtaatctga ttcgatttgt ctccaaaagt tcatattcta
    12901 actcttgctg tagatcaggc gtctctgctt cttcgtttgg ataatacgaa atctcaactt
    12961 ttagtgtacc cgcttctttt cctggaacta cccgatcatt ttctatgtca ggttcttctc
    13021 gataactaat ttctaaaata aagtaacctg gaagttttct atagctgtca ggtgacttca
    13081 cttccttgat ccgatagacc ccttccaaca agtctgaagc attgaatagg gaagaatcgt
    13141 ttggatctgg tgccaagcct ccctcgatga tttcatactc tcctgcacta ttttgtttct
    13201 caattgaaaa ttctgcgcct tcaagacgtc ggctattcat actgtcaatt ttcaaaagtt
    13261 ccaaatcgat tggcgctagt tcatcttcta aggttacttg tagtacacca tcgatgattt
    13321 catagccttc cggcaaggga gcagtttcgt cttcgatgac cgtatcttca atgataaact
    13381 taccttcttc attcagtgca aactggaaca cttgatcaga tagccgatac ccatttggtg
    13441 cgacagattc cgagacctca tattcaccaa tagacagatg cagaggttgg tcgtaattta
    13501 aatcgacata cttttctact cctacggcac gagaatagaa gtatccgatc ccaggcaaac
    13561 gtttcgattc ttctggttct aacggattac cagtgtatgc tcgatacagc gggaaggaag
    13621 aatcccccac tttcctgata tcgaaacctg caccatctaa tgtatggcct tcatggtcta
    13681 attttctaac tcggatctcc cgataatcaa acgtattgta agcagtgaat gctacccata
    13741 aacgatcctc ttcatctgtt gtatacgtga gatattgcca attttcttca cctgtttgct
    13801 tgagttggat agtccctata gcattatcgt aaagtcgcaa gatccatgtg tcattttcat
    13861 caggttcttt gaaaccttct gggttctggc tctcaatgat tttgtattct gcatagttgg
    13921 tagtttcttc actatcctta taaacaaatg aatctggctc tccgattgga tgaataactt
    13981 tctcaccatc cacgattaca tatttcccgt cttcatctac tgaatagaac gtgagtctcc
    14041 ctttctcttc ctctacttcg ttcacaccag aaagcccttt cgctactgga tatccgattt
    14101 ccagtccgtt accatctgtt tcataaagtt caaactctgc gccattgatt ggcatattac
    14161 tgccttcaat tagtttattg atattcatgt ggattggctt gaattcattt cgcatttcca
    14221 gtttgacata caactcatca tactctgtgc cttctatgtt aggctcttgt acaacagaaa
    14281 gcttgtgata ttctccttca taataggaat tcggatcttt accttccggt ccgccgactc
    14341 tcgaaccaga tgtatattca tctacaagct cgtagtgacc taacatctca tcttcgttgg
    14401 ctgcatattt tttgatttgg aattgatacg tctcatctac taactgataa cgtggtggtc
    14461 cagcgatctc tctgatccaa aatatgtcgc tgactgctcc agtttcatct cctaaaatcg
    14521 gcatcttcgt ttcaaagatc gctattccgt ccgtggtagt tttacttgtt tcgattggtt
    14581 cggcttcccc actctctgga ttttggtcat ataatgcaaa ttcgactcca gccaacggct
    14641 cgccattttg acccttcttg ctgacttgga aattacggcc tttatgttcg taacgaatgg
    14701 aagaagatgg gaaatacatc cgtccagtag tgatatcatt ctcataatcc tccaggttct
    14761 gaatataggc catattgttc actgtgcaaa aatttccgtc ttgagctccc gcatcaagtt
    14821 ttacttggta cttgatcctg accatttccc cttcacctaa agaaatcgga gaaaatttat
    14881 aagcttggtt ttcccgtccg acatacgttg tcggagtcgc cactacttct ggtggggctt
    14941 gtccatgacg ttcttcatcc catatttctg gctcttggta cttcatgccg ttttctcctg
    15001 gatctagatg gtaaaactga atatctgccg atctgccagc tccaaatggt gagacattaa
    15061 aaccagtttc atcataaagt gtggcttgct ggatcgttct cttgaaggta gaagagatat
    15121 cctccaaggc tgtaacaatg tcattcttgt tgttcgcatt gtaatatcgc ggtgttacgc
    15181 catcttcttc taaatcagca atatttttca atacatttcg tccaatagca tctagtcgtt
    15241 gtctcccgct aacgtttcca tcgattccta aaccaatgga gaagatactt ggcgggttct
    15301 cttgatactt gtttttcagc caatgatgat aagcaactgt gttgatcgca gatgactttt
    15361 cttgcgaagt aggactgcca ttcgtattgt tattagaaga accatcacca acccagtgag
    15421 tcgctttatt atctccttct tttacctctc cataacgata gtgtacattt acttcatctt
    15481 ctggacggtt aaagtcagat gcataggtaa ctggatgagt aaaccctgca ttagaagtat
    15541 tcccagaaaa ctcctcaaaa tttctgaaca aaacgtcatt atcttcagca atcttatctt
    15601 ccatcacagt ccagttgctc caagctccat tagtgctcgt acgataactt gactgaatcg
    15661 gtgcataact gaaggtcggt gtcccatctc ccacaacgat taggattttt tctggatttc
    15721 tattttctcc gccattgtct ttatacaatg tttcatagcc atttttaagc cctagtgtaa
    15781 gcggagttcc tccgattggt gtacgattta atgctgtatt ctcacttccc gtcaaacgat
    15841 tgatatcatt tgtcaatgga aagatgtccg aactgatttg ctcatggtta ttgatagctg
    15901 tactattatg gtaaaaattc accataccga ttcgaatatc cagactcggg tcagagagca
    15961 gatctttact catttcgttt actgcctcaa tcaacgcagc atttttagtt tgactattgg
    16021 tcccagcggt taattcactc atactcgctg acttatctag aacaaaaacg atatcgattg
    16081 gactgatttc ctttttctcg cccccgatga catctagata gacattgtaa agggttgttg
    16141 catcttcagg atgtagatca gatgctgtgc tgcttggcag aacaggttct acccattttt
    16201 tgatgtacgc cccgttataa tccaagtaac tgttttcaaa attccgttca tttccccaaa
    16261 gctgaacggt ttgagctgga acgtttctat cttcttcgcc tgcagttgat ccattattag
    16321 tagcatagtc gtaatttcgc cagttcgctc ccccgtttgt ataatcacta tcatcgatca
    16381 agttctctcc gtcatatggc gagcctttgt ctccttgata gcgttcattg ctttgtgtcc
    16441 aatttcctaa cacttttgta tagtcattcg ttgtaaactt cgggtagcga tgctgttcgt
    16501 tttcaacttc tccgctagga tagatatatt cataggggtc ttgcgtagct gttggcccaa
    16561 gacgctccaa aataccgttg aataactggc gttgaatgcg ctggagtgct tgaccgctgg
    16621 gtacgattgg ttcgtccagc ttttccatct cattttcttc gctacgttga atttcagcgg
    16681 aatcttctgt atcagtttca ttttcttgta attcttggtt atctattctt tcatcaacca
    16741 ccacttgaag aggtagttac ccttcgttca catcggggat cttttctgtt tcaaaaatca
    16801 atgtgccggt ttctgcttct tttgaataaa cttttccgac ataccattct tgaccatcga
    16861 tcgtctcgac ttttaggtct tctccatctt catcatagga ggatagacct gttccacgaa
    16921 cgttttttac ggtaccaatc ccggcggctg ccgcatcgat tgctaaacgt attctacctt
    16981 cattggcact ttcctttttt tcaaaagcca gatgccattc aagggtttct tctttttcaa
    17041 tatacttccc ttcaattttt gcatagtcct catctaagaa tacctcatcc tcctttgttt
    17101 ctgttgactc agcagctaca tccagggtcg tcatatgata gactgactgg aaatatggtc
    17161 caaatagcaa actgacagtt actaactgca aaagcttcaa ttttaaccct ttcacatcct
    17221 tatactcctt tcaaaaatat ttgtcttctt tcacaacaat caacaaaaca atgcccatct
    17281 aattataatt taatttaatt ttaattttaa atcaaatgat atccttttta gttaaaaaac
    17341 accaattata caacatttat tattaaaatt aaattataaa attttataac gttctcattt
    17401 aacaaaactt aagttattaa gacaaatgaa taaacaacta cgctttcctc gaaacaaaaa
    17461 aataaagcac aaatgcgcat ttacgcactc atgctttatt cgtacaatta attcagataa
    17521 cggcagcgca taggatgcca ataacacttc ctaaaatagc tcctcctaaa atgtcagtag
    17581 gataatgtaa aagtaaaaaa atcctcgaat aagcgatcaa acaagcaaat aaaaaggcta
    17641 atattcctgc aactggactt aattcgaaca acgcagctac tgataaaaag gctagcgctg
    17701 tatgtcctga aggaaaagaa gaagtcttcg gtttacttgt gtaaaggaca agccgacgat
    17761 ttttttcata aggtcttttc cgtcttgtcg tttttttgat aaaaagcaaa ccaacaaaaa
    17821 aatagatcaa actgccgata agaatggcta gaccatattc tctagtatca ggtatcaaga
    17881 ctaaaacagc agcaaggagc aaccaaataa aaccaccatt agagatatta ctgataaatt
    17941 tcaaaaaatt gatttttcga ctatccgttt tccgttgctt ggcgaacttg ttgataatac
    18001 tatcatcgat tttagcaatc aaacgcaaca tgttattcct cctttttaag aaccttttct
    18061 tgcgtacgat ggataaaaag caggacttga aaataaaaca agaaactgaa acctaataaa
    18121 gcaaaataga ttggtcctgc aaaccagaat gcgacaagaa ggatcaacgt gcctgccaaa
    18181 tagaaaagcg cgcttttgaa atcattaaaa aacaagaaaa atgcagtaat ccaagaagct
    18241 tctttttgtt ccagttgaag caagattttg taactaaaga gaagcgcgct taatagaagc
    18301 aaaaacaacg tagtatacat caatgtatag ccaattcccc caccccaata gcggaaaatc
    18361 tgataatcga tcaatagaag aagaaaataa acacttgcta aactatcttg ctttccataa
    18421 agagagaagt tctcacgaaa ctttttccag aatagtgaac cgagcttttt ctcttcattt
    18481 tttttgtaat gataaatcat atacagaact gttcgacttg cagttcctaa tgtgaaaaca
    18541 ggcaagacgc acaggaacca tagtagctgt aaatggataa gacgtggaat ccattcaatg
    18601 atttctaaga aataatcatt ggctttttta ccaatcgatc gattcatcat ccttttgaac
    18661 ctcctgtcaa atccatccct tcaatgaaat aacgttgtgc aaaaaagaac agcaaaatcg
    18721 tcgggatcaa gacaagtgtt gctcctgcca tcagataatt ccattgtgtc gttgcttggt
    18781 tctgaaaaac ttgcaagcca atctgtaacg tgtatttttc ttgatcttgg atatataaca
    18841 gaggacctaa gaaatcattc caagctgcgt tgaaggaatt gatcgcaatg gtaatcaagg
    18901 caggtttcgt caatggaagc atcaaatgac tccaaatata caaatgattc gctccgtcga
    18961 tttcagcagc ttcgatcaat tcattattga tcgataagta aaattgacgc atcagaaata
    19021 tattgaacgc attgccgaag aacgatggaa cgatcagggg caaataagta cctacccagc
    19081 cgatcttgga gaataataca tattgaggga tcatcgttac aaaccccggg atcatcatcg
    19141 tagaaagaac caaagcaaac atcagctttt tcccaggaaa atcaagcttc gcaaatccat
    19201 aagcaataaa agaatttgaa aggacattac cgatcactac tagcacagtg atgaataatg
    19261 tatttcttgc atagcgccaa aaaggaaagg cggcaatcgt atccagatag ttttcccata
    19321 cgattttttc tgggaaaaag gtcggaggat aacgaacgat ttcttccatt ggtttcaatg
    19381 aagtaaacac catccaaagc aatggaatga tcaaaatgat tcctattact gtcatgagga
    19441 cataacttag tatttttttt gtttgccgat tcacactggt ctttttgaaa taatccatca
    19501 tttgccgtca cctccagaat aatagaccca cttaggtgca agcttcaaat tgacaaacgt
    19561 cagaaccaac acgatcagaa acaggatcat tgacatagcc atcgcataac ccatatcaaa
    19621 agctacgaac gccttgttcc acatatgcag attataaaaa agcaaagaat ttgcaggtcc
    19681 gcctgttcca tttttcgtca tgacataagc ttcttgaaaa atttggaaag aaccgatcgt
    19741 agaggtgatc acatcgtaaa aaataatagg agtgattaat ggcaaagtaa tacttttgaa
    19801 ctttccccaa acgcctgctc catcgatttc tgctgattca tataaagatc taggaacatt
    19861 ttgtaatgta gctaagtaaa gtagcattgc tcctccagaa ctccatattt tcatcaaaat
    19921 caacgcaggt ttcgtccatt gtggatcaaa cagccaagct ggtccaggga tatggaacca
    19981 gcccagaaat gtgttgatca atcctgcaga tggagaaagc agctgcatcc ataagatgta
    20041 gactgctact cctgataaga ctgccggtaa atagaaagct gtacggaata cactgattcc
    20101 tttgattttt tgattgagta ctgtggctaa aaacacactg aaaatcgtcg tcagcggtac
    20161 attgaagatg acgaaataaa cggtgttgta taacgatttc caaaataaat catcgtgaac
    20221 gaacattctt ttgaaatttg ctagaccaat gaaattcatc ttggatgtca aattataatc
    20281 ggtgaaacta ccaataactg aaaaaagcat aggccctagc ataaatacca agaacccgat
    20341 aataaaaggc gagataaata aataggccca tcttgcttct tttttctttc ggatagataa
    20401 acgcttcaaa ttttcttgcc tcctctgtac ctagacagtc aaaaatggat tattgatttt
    20461 gcttgactaa gttttctact gctttttgtg catctgccaa tccttctttt ggtgattttg
    20521 ttcctaaaag aatctggtca atctgttcat tcactaatgt cgtgtaatcc ggagcatcta
    20581 aaggaactgg tgtgatcacc gtatttttaa agttttcgtc cgccatttga tagatcattt
    20641 ttcctttatc cgttaggttg tcattattca ctaaattctc attggctgtc ttgctcgcca
    20701 tgatatcaaa acttttttcg ccgaattttt cttgtacttc tggagaagaa agatatttga
    20761 tgaattcata agaggcctca ggatcttttg ctccgtatgg tacttccaag acgaatccgc
    20821 caccccacga ccaatggcct gaaccttctt ctttttctgg gagctgcatc acgccaaaat
    20881 caaaatcatc cggtgcattt tccatcaaac tagagtaata attgatattt tgggcacgca
    20941 tggctactaa tccggaaata aatggatcag atacaccaga cccaaattcg gcttctaatt
    21001 tattgatcgt atttttccca taataatctt gccagtcttg gatccactcc aaggcttcca
    21061 ctttattttt agtatcaatc ttgacttttt catctttgtc aaaccaacta gtcccgttat
    21121 ctgcgtttag cgcccataca tcagccccta aattccataa tggataaaaa ccgatgcgtg
    21181 taaattcttt tccttctttt ttatctagtt tacgtgctgt ttcttctaat tcatcccaag
    21241 ttgtcggtag atcttgttca ctgattccag cttctttaaa taacttttta ttataaaaaa
    21301 tgacttgtgt atctgtattg aatgggatcg cataaggatc accttcatat tctactgtat
    21361 cccaaagctg cggataaaaa tccttagaag tgtctttttc tacatatttg gagagattag
    21421 ttgcttgctg tgcttctgct ctttgggcaa cggaatttat gtcctgtaca ataacatcgg
    21481 gtgcatttcc agctgttatt gcagataaag atttagtcca gatgtcaccc catggctgat
    21541 acttataatt tacatggatc tcgttttgtg attgattgaa atcctcaatg atttcttcga
    21601 tagcttcttt tcgtgcaccg gatccccaaa aggaccagaa atcaatattt tttcttccat
    21661 cacttgtttt ttcagaagaa tttgctgcac ttgaactggc atcgcttgca ttacttgatc
    21721 cgcatcctcc taataaaaac aatgctgaac ttgccagcaa ccctgtaaac aataattttt
    21781 tcatatttta cctccaaatt ttaatgtaac cgatgccggt tttaatataa cgatatcatt
    21841 ttgttctgaa aataacaatt tataaggtat gattctaaaa aaattattaa tgataccaat
    21901 actttatata attcgcgagc gctttctttg aattttttaa tctttcgtag cagaatgaga
    21961 atgtattaaa aatagattag gaggaatttt gatgtttaga caccagaaag aattgcagtt
    22021 tgaagcaaaa ccagatcgtc cgaatccatt aatcgcgaaa tatctgcagg agctgatcgg
    22081 cggccaatat ggcgagatga gtgtagctat gcagtacctg ttccaaggct ggagctgccg
    22141 aggagaagaa aaatataaag atatgctgat ggacattgca acagaagaaa tcggccacgt
    22201 ggaaatgttg gcgacaatgg ttgctcgttt attagagggt gcgcctgttg aagatcaaga
    22261 agcagcgatg caaaaagatc caacaatcgg tgccattatc tctggaatga atccacagca
    22321 cgcgatcgta gctggcttag gacctcgtcc tgctgacagc gtgggcaatc catggagcgg
    22381 tgcctatatc attgcgagtg gcaacttgtt agcagacttt agagctaatt taaatgccga
    22441 atcacaagga agacttcaag tagcacgtat ttacgaaatg atcgatgatc gtggcgtaaa
    22501 agatatgctg tctgttctcc ttgcaagaga tagctatcat caaaatctat gggaatgtgc
    22561 agtgaaagaa ttagaagaaa aagaaggttc aatcttagtc ccaagtactt tcccgcgtga
    22621 caaagaacga aaagacattg cttatgactt ctataacttc tcagagggag accaaagtag
    22681 tgaaggttct tgggctagtg gcaaagcact ggatggcgaa ggcgaatatc aatggttgaa
    22741 agaacctaag atcgaaggcg ctgcaccaaa attagaacca gtaacaccaa aaatgtatgg
    22801 gacacctcca ttcaagaaat aaatatctac ccgtagaaac acccgttttt tgttccacac
    22861 gctttgtgag caaaactgac taaagtcatt aatcataacc acccttcaaa agggtggttt
    22921 tctctagagc tataagctca aatcaccggc cagcgcctaa agacgctggc tttcatattt
    22981 tatgttcaag ccatattgcc tttgccagtt ttgcccacct cttaaaaggt ttatgtacta
    23041 ctccttagcc cttaaaagga tcgtcttact ctcgtacact cagcttatct aacgcactat
    23101 catgtttttc ttgttcttga atgtactttt tgatggtcgc ttcattttgt cctacttttg
    23161 tcacatagta tccttccacc caaaagtgtc gatacccaaa tttatatttt agatttgcac
    23221 aatctcttga tgatctcctg aatattggct cgatattgat tataaattat ttttcttcta
    23281 tactttggtg tgaatacaat gtggtattta cacatctact ttgtacgggc taaactattt
    23341 ccctttattg ctccacccgc atagcaagtg gttttgtatt tcaatcgctc ggcgcgctca
    23401 attaggtcta aagacaataa atctaaaaac ccgctgcttc actgacagaa acaacgggtt
    23461 ttcagaaaaa aatgaaatga aaaagatctc ttacggcaat ctattttatt tttttgattt
    23521 tttataaatg gaaaatgctt caagaagtcc aagaaataaa gccgtataaa aacaagaacc
    23581 tatgaaatct ttatttatta gatattcaac agaaatcccg ataacactag ccaacaatat
    23641 atttatcaga agtgtaaaat aaggatgctt tttatagaat agaactagtg ttgttttcat
    23701 agactctcct ttcttcatat ttccaattaa tcacaaattc acggcattac tagaaaaatt
    23761 aaaggggcgc agaagtggaa aaatactctt cgactttccc tacaaaccag gccatcttgt
    23821 gtttaataac tataccaaat ctaaggtacc tctaaatcta aattccattt gtggcggtga
    23881 cctccaattt ttaactcttc tatctcccat ccagtataca taaccttgat acttcgacca
    23941 cttatatatc gaacacacaa atacctatcc attgcaggag tactacctaa cttagttgct
    24001 acttgcatct aaataacagc tgaaaagata ggacttactc ttagtaaagt atctgaacta
    24061 ttaattttta aactatcatt tttatcctca actacatgca catctgttgc aacttcactt
    24121 tctgtctcat ccagataaat gattaaggaa tcgggaattg caatatcttc agctgacgct
    24181 ggaatagttg agctaaatta gtcatcatat ctttattata aaataaccaa cagcaaaaaa
    24241 tacaatgaaa aacaaacttg gaactaatgt atcattaata aatgttgaaa aattcagcaa
    24301 ctcattactg tatgatttta gcagcccaac caacatatac cctataaaag ctaataagat
    24361 ggttaggaat cctttagaat ttttactcat tcatagacac ttccttaaat aacttttatt
    24421 atagaaggta ttgtaaataa taatattgac aaaaagtgac tcaaaataaa aaaaacctta
    24481 cattatactg gaatgaactg tattgatagt gtacctaaaa gctctactga cataagatcg
    24541 tgtaatcggt aaagtaattt tattaattat acgggatgta atcaattaga gaataaacta
    24601 atcataaaag gtacttttac attcataatt gtcgctttga gattctactt tggttcacta
    24661 tctgacccaa ttgcacattt tcgatcatta caagtattgg tgtcgttaga acaactaaat
    24721 aaaaactagc ggtagatttt tcagaatcta tgctaatttc tgtatggatg ttccagtgat
    24781 ggcaaatttc tcttttcatt gtttttcttg tcgcatgata tgcctttttg tgttattcag
    24841 ttaaactata gaaattcttt cctagacaga tcttgatccc aaattttacg tttttgagca
    24901 tcgatatagt ttaaaatgac attactcata tctaaagagt ttagattttt taggactaaa
    24961 actgtcagtt gttctttttc ttctttctgt taaagtattg tcgcgacttt tcgtatacgt
    25021 taagatatgt cgtgaaaaaa caagttttgt gacacaaaaa aacgaatgac ttgaggaaat
    25081 cacctcaatc attcgtttaa aatgctatat attctttagt ttaagatacg gaaattaatg
    25141 ccatcgacca cactagtaga atgaacgaca tttacttggc ctttttttaa tttcgttatg
    25201 tttagcatat aaaaatatta tagagtttac ttacaatgct taaaacatat actgtataat
    25261 ctgatcaatt ttagccatgt cttgcaatgg taagttttct atttttttga gttttcgttc
    25321 cttaaaatca agagatttta attgtgtaat aattacttgt ccttttatat ctagctcttc
    25381 aggtaatgag taacgtgtcg gtaaattctt tatagtggaa gtaattggac atatgaccga
    25441 aaacattgtt ttacggttaa attcataacg agcgacgact agtcctggtc gtcttttttg
    25501 aatttctttt cctacggagg gatcaaaatc aatccataca atatcacctt ttttaggaat
    25561 ataaattctt tcttctgaca ctataaaatt tctctccctt caggagttaa atcttcccat
    25621 tcgtctttct cataatactc ggcctcttcc ccaccactaa atggatcttc aatttttgga
    25681 actaacgtaa ttgttccatc atcagaatac acaacgatat attcttggtt ttctgaaggt
    25741 ttctttccgt tatcagaagg taaagttacc actacagaac ttccttgtaa tctagatttt
    25801 gtaacaagca ttatcatcac tcccttaata aaagtatact atataacact aagtaacgca
    25861 aaattcccct ttgtaaacta tctagaaaat tgttttgttt aaagtatatc ttattggata
    25921 taaatagaaa taaattgtag ccagttcatt ttttctttaa ctaaaaaaat aatccaagaa
    25981 acattgaacc tatggatcca aaagctaccc aaatactata gctaaccccc cgctaccttc
    26041 aattttgcag ataaaacgct ttgggagaaa atttcaaaga caacaggcca tcctatcatg
    26101 cccgattata aacaaaaagc tggctattcc ttttgctctg acaaatttca attgggaaag
    26161 ttcacgaaac tagcaggtga agaagtttca actgttcgga tcaaagaatg cccgcttcaa
    26221 atagaaacat tggttcaacg aatttcaact agacaagact ttgctattgt agaacgtgag
    26281 atcgtagcta tttttgtaga agaagggatt ttacatgatc caactcatat caatgtggat
    26341 aaatggcaac ctcttatcta taaattcaga gaatatacaa ctgccaacca gtcgttaact
    26401 ttgaatttta gattccaaga attcaaagag tcataatgat aaacatcgtt aggtataaat
    26461 aaaaaagact cgcagcacga gtaaaaaaca aatagaagct atacattaga aaggtcgact
    26521 gtgaccctat agatgcagta tatgcagctt atgagcattt cttctgttct ctttgttttt
    26581 gggatctgtg ctgaagtctt tttcgttttt tgccgctctt tctttgaaaa atcgattatg
    26641 ttataccttc tccattacta attcaggcat ctgctgagta atgatttttc gcgcaaaaga
    26701 aatatctgtt acccaaggtt cgatttgttc ttcagtcaag acaagcggca ttcggtcatg
    26761 gattggtgcg actgtgcggt taggttcagt agtcattatg attgattctg ccattttttc
    26821 atcctgatgg ctacgataaa aaccgccaat atacactatt ttttccttat ttgaaaacat
    26881 aaatttgtct ttttcttcat tccactcatt gggaaaacac aacgattttg caaaaatggt
    26941 tgatgaaagg tctttttttc ttctactgtc tctgcacggg cattaatcat caattgtcct
    27001 tttttaaatc cggtaaatcc ccacttcatg atttccggag tgaggatgtt ttcttcctct
    27061 tttcttaacg tcacaacaag atttgacgga aaaacttctc cagctgctaa ttctgctgcc
    27121 tgcttcggtt gtttcgtctg ggcttctcga taataattct gaaggagctc tgactctaga
    27181 tcaaaaaaat aacgaccaca catcctaatc cctcccaatc tttttttcta tcatacactg
    27241 caacaagaag ttcgcaccaa caaaacgctt tcaatttttg tagaactaaa aaagttgtga
    27301 caagtgacaa actctccagt gaatggtatt ctcctgccaa ctcctcggtc acaagtcaca
    27361 atattcaaca aacatgagaa gctgcttttt gaaattgttc cttgtgactc ggagtgaaac
    27421 ggcaggctta cgacctctgg gtaaacggtg ttcaaaagcc agtttctcgg tattaagcca
    27481 aatttatgca aaaatgtaga aagctatttt cacaaaattt ttcttaatac tcgaaactaa
    27541 gcgacttttt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    27601 gcccaaccaa acgaaaaata tgagcagcta tttttatttg gctgttctta ttacttgatg
    27661 cagggcagct ttttcacaac ctctccacta aacggtgttc aatcagctaa ccttcggtat
    27721 tagctcaaaa aacggcaaaa tatgaggaac tattttgccg tttgtttctc taatactcag
    27781 gtcaaagcgc tgatttcaca acctctaata cacggtgttc cagaagtagc ttcttcga
//
LOCUS       DAYYKN010000023        27671 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000023 DAYYKN010000000
VERSION     DAYYKN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.023"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1897
                     /gene="yfcC"
                     /locus_tag="ACHBB7_000854"
     CDS             158..1897
                     /gene="yfcC"
                     /locus_tag="ACHBB7_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM9208824.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2054..3238
                     /gene="iadA"
                     /locus_tag="ACHBB7_000855"
     CDS             2054..3238
                     /gene="iadA"
                     /locus_tag="ACHBB7_000855"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM9208825.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3349..3552
                     /locus_tag="ACHBB7_000856"
     CDS             3349..3552
                     /locus_tag="ACHBB7_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9208826.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3653..4226)
                     /locus_tag="ACHBB7_000857"
                     /pseudo
     CDS             complement(3653..4226)
                     /locus_tag="ACHBB7_000857"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(4329..5831)
                     /locus_tag="ACHBB7_000858"
     CDS             complement(4329..5831)
                     /locus_tag="ACHBB7_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9208827.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6379..8058
                     /locus_tag="ACHBB7_000859"
     CDS             6379..8058
                     /locus_tag="ACHBB7_000859"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9208828.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8185..8664
                     /locus_tag="ACHBB7_000860"
     CDS             8185..8664
                     /locus_tag="ACHBB7_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM9208829.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8883..9563
                     /locus_tag="ACHBB7_000861"
     CDS             8883..9563
                     /locus_tag="ACHBB7_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM9208830.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9571..10902
                     /locus_tag="ACHBB7_000862"
     CDS             9571..10902
                     /locus_tag="ACHBB7_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM9208831.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10917..11879
                     /locus_tag="ACHBB7_000863"
     CDS             10917..11879
                     /locus_tag="ACHBB7_000863"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM9208832.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11990..12328
                     /locus_tag="ACHBB7_000864"
     CDS             11990..12328
                     /locus_tag="ACHBB7_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM9208833.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12407..13114
                     /locus_tag="ACHBB7_000865"
     CDS             12407..13114
                     /locus_tag="ACHBB7_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM9208834.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13217..14698)
                     /locus_tag="ACHBB7_000866"
     CDS             complement(13217..14698)
                     /locus_tag="ACHBB7_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9208835.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15007..15543
                     /locus_tag="ACHBB7_000867"
     CDS             15007..15543
                     /locus_tag="ACHBB7_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9208836.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15621..16991
                     /locus_tag="ACHBB7_000868"
     CDS             15621..16991
                     /locus_tag="ACHBB7_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM9208837.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17058..17393
                     /locus_tag="ACHBB7_000869"
     CDS             17058..17393
                     /locus_tag="ACHBB7_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208838.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17474..18331)
                     /locus_tag="ACHBB7_000870"
     CDS             complement(17474..18331)
                     /locus_tag="ACHBB7_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9208839.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18438..18953
                     /gene="tadA"
                     /locus_tag="ACHBB7_000871"
     CDS             18438..18953
                     /gene="tadA"
                     /locus_tag="ACHBB7_000871"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM9208840.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            18976..19060
                     /gene="ffs"
                     /locus_tag="ACHBB7_000872"
     ncRNA           18976..19060
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHBB7_000872"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19270..20655
                     /locus_tag="ACHBB7_000873"
     CDS             19270..20655
                     /locus_tag="ACHBB7_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM9208841.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20692..21369
                     /locus_tag="ACHBB7_000874"
     CDS             20692..21369
                     /locus_tag="ACHBB7_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM9208842.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21377..22141
                     /locus_tag="ACHBB7_000875"
     CDS             21377..22141
                     /locus_tag="ACHBB7_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM9208843.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22143..22802
                     /locus_tag="ACHBB7_000876"
     CDS             22143..22802
                     /locus_tag="ACHBB7_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM9208844.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22832..23341)
                     /locus_tag="ACHBB7_000877"
     CDS             complement(22832..23341)
                     /locus_tag="ACHBB7_000877"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM9208845.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23453..24451)
                     /locus_tag="ACHBB7_000878"
     CDS             complement(23453..24451)
                     /locus_tag="ACHBB7_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9208846.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24652..25236
                     /locus_tag="ACHBB7_000879"
     CDS             24652..25236
                     /locus_tag="ACHBB7_000879"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9208847.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25313..26488)
                     /locus_tag="ACHBB7_000880"
     CDS             complement(25313..26488)
                     /locus_tag="ACHBB7_000880"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM9208848.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            26570..26950
                     /locus_tag="ACHBB7_000881"
     CDS             26570..26950
                     /locus_tag="ACHBB7_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM9208849.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27024..27386
                     /locus_tag="ACHBB7_000882"
     CDS             27024..27386
                     /locus_tag="ACHBB7_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208850.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         8990 a         4676 c         5770 g         8235 t
ORIGIN      
        1 aaaaaaataa tcatacatag gagaaaaaaa acaaaataga aaaaagtgat tttctaataa
       61 attattcatg tggaaatgtc atttttactg tataattcat ttgattatcc attttttatt
      121 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
      181 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      241 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      301 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      361 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      421 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      481 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      541 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      601 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      661 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      721 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      781 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      841 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      901 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      961 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
     1021 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
     1081 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1141 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1201 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1261 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1321 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1381 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1441 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1501 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1561 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1621 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1681 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1741 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1801 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1861 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1921 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1981 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     2041 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     2101 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2161 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2221 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2281 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2341 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2401 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2461 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2521 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2581 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2641 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2701 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2761 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2821 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2881 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2941 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     3001 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     3061 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3121 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3181 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3241 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3301 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3361 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3421 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3481 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3541 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3601 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3661 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3721 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3781 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3841 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3901 aaagtttggt cacctgtttt ttttgctaat atttcatcaa ttcctacacg agccagtcga
     3961 tctaaagcaa cttgtcgata attctcttgc ttggtcttag tgatttccat tgctgctatg
     4021 acgttgtcta gagccgataa ataaggaagc aagttgtaag cttggaaaat aatcgagaca
     4081 tcatttttac gataacttct taacccgatt ttgttcagtg aacgatcttg atagtatacc
     4141 tttccttctt ttggtgcatc tagtccagaa ataagcgaaa gaaaagtcgt ttttccagaa
     4201 ccacttgctc ctaaaatagc gtacattttt ccagcttcga aattcagttc tacatcttta
     4261 taaagcgcat cttgcggatc ttgataccaa aatcccaatt gttctgtccg taacatcaaa
     4321 ttcctccttt agtttaatag gatttttctt gggtttaatc ttaatatccc tatagaagct
     4381 aaaaagacag aaaacagact gattccaaat gctactccgg ctaatagtcc caattgtgca
     4441 ggtttgacat tgatttccaa ttcgctgact tgatcagaaa cagcaaatgg attattcgtg
     4501 gttccttgtt gaccatcgcg ttgaccgcct tgttcttgtc tgttacctgg catttgtcca
     4561 ccgccaggag cttgctggtt ttgtgtcgtg gtttctgtct gttggtcaag tagctgctgt
     4621 cccacggcat tcgcgacgac gtttccgcta agcgaagcaa ctcctaatgc aagaatcata
     4681 caaatagcca cttcggtaaa caattggaga atgattttac ttctggattc tcctaaagat
     4741 agcaagacgc ctaactcatg ccgacgctcc cgaatggtaa tcataatgat cagagtaagt
     4801 atgataatac cggctacagc cactagtaaa acaacatttt tagaaaaact tgccacgttg
     4861 tttaaaggtt ccaacattga ctgatacatg gaatcatttg attgtaagct aaatgtatct
     4921 gtgtcgatca gcttctcagc ttctttgaga aactcatcca ttttatctgg atcagataga
     4981 gtgtaaatag cagaatctat cgtgttatcg tcagatgtcc catttagttc atttgcaaaa
     5041 gtgtaagaag tgtaaagtgt attcgacgga ttcatgaagt tgaatttcat acccatactg
     5101 ccgcttgttt cgctggattc ataaattcct ttgatcgtca tttcataagt ggtatcttca
     5161 tcttcagtac tcgtgattgt gaaagtatct ccgactgcca ggtcatttgt ttctgctaga
     5221 gtagaatcaa tgactacatt gtttgtgcct ttgtcgctgt catctattcc ttcaccatcc
     5281 gtgatttttg ctgttccatc agaaaaatta ctattttgtg cagttgctga cacaccactg
     5341 atttgaaagt ctgcttgagt catttgcgga ttcccgccca ttcctccagc aggttcaccg
     5401 cctgcatcag tagtttctga actgtcttca cttgaatcag aactagtgat ggcagttatt
     5461 ccatcttttg ccatagctga ggcggaactt tcaaaggaat aactctttac attatctaat
     5521 tcagctattt tttccgcgtc cgttaaggaa actggtgtaa gagaaaaact gcctggatct
     5581 ggacgttctt cagtggtaga accgcttgaa gaagtttgtt cctgtttttt aaatgaagct
     5641 tctcgattgg cacttaaagt cactgttgct ccaacttctt tttgtgcttg attcgctgct
     5701 tcttctgcag cgcttcgaat cgtgaggcca gctagcacaa aaataagaat ggctgtgaac
     5761 acggcaaaca ataacaatga acgtccccat tttacttttg tacttttcca tgcacgtttc
     5821 ataaaattca tattttgcat ccctcctagt agatgcattt gataatagac aacctatctt
     5881 aaagctagct taaatcttat gctgtttgca caccatcaac atgaggttct gtatatataa
     5941 aaagtcacta tagttattgc aaaaaatgtg gtaaaatgaa aaaagtatgt ttcaaaccga
     6001 cgaagaagaa atgaaagagg aatagcaaaa gaaatcctag atgctacaga gggattttta
     6061 cgtgcatagt tgttctaaca attgctgata ctagcgattt agtttatttg ttgtataata
     6121 atggggaaca gctgaatact tatataagta ttctggctat agcgtttcgc aaaagatcca
     6181 ttttgttaat gaagtatatc caaaataaaa tgcccatcca actgtcagaa cgcggatgtg
     6241 ctggtaattt tcctgctgat tctccataag ttgagaaata gtgattttgc atatgcttca
     6301 ttggaatggc tcatatattt agataaagaa gaaaatcgac atctttaaac ttggatgtca
     6361 taagaagagg tgaagtttgt gagtaccatt aaaattatcc cattaggcgg cgttcgtgaa
     6421 aatggaaaaa acatgtatgt cgcagaagtg ggcgaggata tttttatttt agattgtggg
     6481 ctaaaatatc ctgaaaatga gttgttgggg atcgacgtcg taatccctga ttttacgtat
     6541 ttagaagaaa acattgatcg tgtagctggt gtctttttaa cacatggaca tgcagatgcg
     6601 atcggggcac ttccttactt gttgtcaaaa atttctgtac cagtattcgg gacagagttg
     6661 acgatcgagt tagcaaaatt gaatgtcagt cgtaatgaag cagccaaagg atttaaggat
     6721 ttccatgtgg tagatgagca tacagaaata gactttggtg aaacagtagt gagctttttc
     6781 cgtaccactc atacgatacc agattcagtg gggatcaatc tgaaaacaaa agaaggtagt
     6841 atcgtttata cgggagattt taaatttgac caaacggcta tcccaatgta tcaaacagat
     6901 tatgcacgat tggcggaaat cggcaaagaa ggagtcttag ccttactaag tgaatcggca
     6961 aatgcagaaa atcctcagcc tgtagcttct gaagcccaaa tagcagatga agtatttgat
     7021 acgatcaaat attgggaagg gcgtatcatc gtcgcttgtg tagcaagtaa tttgcaacga
     7081 gtgcaacaag tattggatgc agcagaaaaa gcacaacgaa aagttgtctt gactggtcaa
     7141 gatttcgaac gaattattcg cacagcgatg cgtttagaaa aacttcaatt accaagtgaa
     7201 gatctgcttg ttacgttgaa agacatgaaa aactatgatc cagaagaact gatcatttta
     7261 gaaactggcc gaatgggtga acccatcaag tctctgcaaa aaatggcaaa cggtacacac
     7321 cgtaatgtca agattgaaga aggagatttg gtttatatca ctactacgcc aagcatcgcg
     7381 atggaaacca tcgtggcgaa aacggaagat atcatttatc gagctggtgg aacagtgaag
     7441 ttgatctctg agaatatgcg cgtttctggc catgccaatc ctaatgactt gcaattaatg
     7501 atcaatttga tgaaaccaac atattttgtg ccagttcaag gagagtatcg tgaattagct
     7561 gctcatgcag atttggctca tgcagtaggg atgccataca aaaatatcta tattactgga
     7621 cgcggagacg tgctggaata taagaacaag cgtatgagtg ttgcaggaac tacaagtgca
     7681 gaaaatgtaa tgattgatgg attaggcata ggtgatatcg gaaacattgt tttacgtgat
     7741 cgaaaaatct tgtcagaaga cgggattttc gtagcggttg tcactatcag tcggcgagaa
     7801 aagaaaatca tttcaaaacc tcaaattact tcacggggat tcgtttacgt aaaagctagc
     7861 cgtgatctga tgaaagaaag cagcaatatg attgaaacaa tcgttgagaa acatttagaa
     7921 agcaatgatt ttgagtggag caaattaaag caagacatcc gagatcaatt aggccgttac
     7981 ttatttgaac aaacaaaacg ccgcccagtg atcttgccag tcattatgga agctactcaa
     8041 cgtcgaaata aaaaataaac aaaaagagta agatgaacct ctctatagag tgctcgtctt
     8101 gctctttttt tgcgaaaaac aaataatttt tgaatacagc atccctcgct ttataataaa
     8161 cgatagaata aagggagtga atcaatgatg agtcaaacaa aagtcgaacg ttatttagaa
     8221 gaacaaaaga tcagctatca accacttgat tttagtcatg tggtagaaga atctttcaca
     8281 gaagaactac aaaaaagagg gattgatcca gctttgatat gtaaaactct cgtagtgaaa
     8341 ggaaacaaaa ctggggtgat catcgcattg attcctttga acgatcactt agattacaaa
     8401 aaaacgagac aagtgacgaa agatcgtaaa gttggttttc ctggaatgga ttttgtatta
     8461 atgcatacag gatatcccca cggagcaaac acccctattg gaataaaact cgcacatcct
     8521 gattattgtt ttttagcaga cagttcttta aaagaaaaag aagaagtaat tgtgtcaagc
     8581 ggtgaaatcg ggagaagcct tcggataaaa gtagccgatt tagaaaagat cgtccatcct
     8641 atttacgctg atttaattag ttaagggacg agaagagaag ctaagcagag cagagacctc
     8701 aaacttgaga ttatatctgc ttggtacttg gtgcatcctg tattatctgt tacaattgct
     8761 ttgtacatac tgtactatca atccaatgaa aatatattta gcagctatat tttttcattg
     8821 atgtgcaagt gatgaaatcg tttttaataa aaaagataca gagaaaacgc gaggtgtaaa
     8881 atatgaaaat tacgtatcat ggacattcct gtttaacaat tgaaatggaa aatggccaac
     8941 gcgtgatgat tgacccgttt attacaggaa atccattgac agacttaact gcagaagaag
     9001 taaagacaga ttggttgctg attactcatg gtcatagtga ccacatagga gatatgattc
     9061 ctattgcaaa agaaaatcaa gccacaatta tcagtattgt tgagattgct gattatgcga
     9121 acagcagagg agtagattca ttcggtatga atatcggtgg aaaacatgcg tttccattcg
     9181 gcgtggtaaa attcgtacat gcacagcata gttcaagcta tgaagtagac ggtatcgttc
     9241 agtatatggg cgagcctagc ggaattatta ttcaagcaga aggaaaaacg atttatcatg
     9301 caggggatac tgcttatttc agtgatctag gattgcttgc agaagaattc gatattgatg
     9361 tcgctttctt gccgattggc gataactata cgatggggcc agaagatgca gcaaaggctg
     9421 cgcaaacaat tcgtgccaag aaagtagttc caatccatta caatactttc cctgtcattg
     9481 aacaagatcc cgaatctttt gtagagttac tggaagatag tgaaggaaaa ataatgagcg
     9541 tctcggaaac gctggaagtg taggcagaaa atggcgacaa aacatgattt gattttagaa
     9601 tatattgaaa gtttacctgt tggcaatcga atttctgtaa gaagcatcgc aaaagaattg
     9661 aatgtcagtg aaggaacagc gtaccgtgca atcaaagatg cagaaaatgt cggactagtt
     9721 tcaacaattc aacgagtagg gaccatccgt atcgaacgca aattaaaaaa acatattgaa
     9781 cgtttgacat ttggcgaggt cgtcaggatc attgaagggg acgttttagg cggggcggca
     9841 ggattagaca aggtcttgaa taaattcgtc atcggcgcga tgactaaaca tgccatggaa
     9901 cgctatatta cgccaggatc actaatgatc gtcggcaacc gggcggaagt ccaaaaatta
     9961 gcattggaag atggcgccgc agtattgatc acaggaggat tcaatacgac tgctgagatt
    10021 gcaaagctag ctgatgatct tgaaatgccg atcttacgca cctcttatga tacatttaca
    10081 gtcgcaacca tgatcaaccg agcgttaagc gaccaattga tcaaaaaaga tatcatgctt
    10141 gtcagtgata tctatatgcc agctgaaaag actcattatt tgcagcagac agatacgatt
    10201 tctgattatc agcgtttgtc agaagaaacg cagcattcgc gttttccagt cgtcaaccgc
    10261 catcatcgtt tgatggggat tgttacggca aaagatgttt tagggaaatc gccaaaccag
    10321 atcatcgatc gggtgatgac gaaagaacca atcagtgtaa agaaaacaat gagtattgcc
    10381 tctgttagcc atcaaatgat ctgggatggg ctagaagtca tgccggtcgt ttccgatgac
    10441 ttgacattgg aaggcttgat tacccggcaa gatgtaatga aagcgatgca gcttgtccag
    10501 cgacaaccgc agattgcaga tacgatttcg gatcagatat ctggaaatat ccatacgatt
    10561 gatactgacc gtgaaggaaa tcgtttggaa aaccctaagt tcaaattcgt agtagcacca
    10621 caaatggtca atggcgttgg aaccatttct tttggcgtac ttagcgaaat catttctgat
    10681 gtcgcacaaa aaacaatggt gatgaaccaa aaacgaaata tcttgattga acaagtgaac
    10741 ctacattatt tgcggctgat ccaattggaa agcgaattag acattcgtcc acgtgtattg
    10801 gaaatcggca gacgttccgc taaactggat atcgaagtgt ttattgaaaa caccatcgtt
    10861 tccaaagcaa tcgttgtttg tcaagtaatg gaacgttctt agaaagcagg agtgaaatga
    10921 cagtacagca agaaatagta gatacaataa aaaaatacga taaaatcatt atccacaggc
    10981 atttgcgccc tgatcctgat gcattaggat cacaagtagg attagccgaa ctattacggg
    11041 aaagtttccc agaaaaagaa atacgtcaag taggtgaaac gattgaaggg cttcgctttt
    11101 tagcagaaat gcaagaagtt gaagatgtct tctaccaagg agcgctagtc atcgtgactg
    11161 atacagctaa ttcacccaga atcagtgatc aaagatatca gttggcagaa aaactgatca
    11221 aaatcgacca tcatcccaat gatgaccctt atggagatct tgtgtgggta aatacaaaag
    11281 caagtagctg tagtgagatg attgcagaat ttgctttgat gtttcctgaa tctttaaaga
    11341 tgaacgttaa ggctgcacgg ttactttatg caggaatcgt cggagataca gggcgctttt
    11401 tgtatccctc cacgacgtcc cgtacattag agattgcttc gatattgaga aattatccat
    11461 ttgatgcaag tgctttgaac agagagattg aacaaatgcc gatgaaagtc gctaaacttt
    11521 ccggttatct ttatcaaaat atccaggtgg atgaaaatgg tgcaggaaga ataatccttc
    11581 cgcaagaaat tcttgataaa tatggaatcg atgattcaga gacagcttcg atcgtttcac
    11641 ttcctggagt gattgacgaa gtgctggctt gggggatttt tgttcagcag ccttcagggt
    11701 attaccgcgt acgtttacgt tctaaggggc cgatcatcaa tgaattggct aaacgccatc
    11761 atggaggagg ccatccatta gcaagtggtg cgaatgcaaa agatttagca gaagtagagg
    11821 aaatctataa agagatccaa gatatatgcc aatcgtataa gagcagaaaa acagaatgat
    11881 acttcgttcg aaaacgtgcc ttttgtagta aaaatcacaa atgtatcttt tcaagatctt
    11941 ctttttccgt tacaatagtg ctcactaaag attttaaagg agaaagaaga tgacattacc
    12001 aaattgccca gaatgtggct cagagtacac atatgaagat cgaggcttgt tgatttgccc
    12061 agaatgcggg aacgaatgga acaaagaaga acaagaaact gtcatagaag acggattagt
    12121 ggtattagat gcaaatggaa accgtctgtt agagggcgat agcgtgacag tgattaaaga
    12181 cttgaaagta aaaggtgcgt caggtgccat taaacaagga acaaaagtca agaacatccg
    12241 tttagtagaa ggcgatcata atatcgattg caaggtagaa ggatttggac cgatgaaact
    12301 gaaatcggaa tttgtgaaaa agaattaaag aatattcaag gtcttttcac attcttttcc
    12361 tactactaat gataaaataa agtaaacgaa aaggagtgaa acgaaaatgt atggttatgg
    12421 aatgtatggt tttttagatc cgacatttct tttggtcatt cttggattag ttatttccgg
    12481 aattgcgtca gcgtacgtca atagtacttt ccgaaaatac gatcaagtga gaagcaaaaa
    12541 caatgtgact ggtacacaag cagcacaata tatattacaa agtcaacaga taaataatgt
    12601 cggtgttcaa caaattgccg gagatctcac agataactac aattcaggta ataagatgtt
    12661 gagtttgtct caagcaactg cacagtctac ttcagttgct gcgataggtg tagcagctca
    12721 tgaatgcgga catgcggtac aagatgcttc gggttatgtc ccattgaaga ttcgtgcagc
    12781 acttgttcca gcagctaact ttggttcgat gatttcgttc cccttgatta tggtaggtgt
    12841 attgcttagc tggaactcta cgttaatcaa tatcgggatt ttcgcttttt ctttagcatt
    12901 acttttccaa ttggtgacat taccagtcga atttaacgcg tctcggcgag caatccaaat
    12961 tttgagtgaa ggcggactac tgacggaaga agaagtaccg atggcaagac acgtattatt
    13021 tgcagcagca ctgacttatg ttgcagcagc gctttcgaca ttcctgcagc tgttgcgctt
    13081 gatcttattg ttcggcggca accgaagaga ttaaaataaa gaagttgtga aatcagcgct
    13141 ttgatctgag ctaataccga gagttagctg attgaatacc atttatttgg aataagagga
    13201 tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt ttcatataca
    13261 gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt gtatccagat
    13321 agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc aaatcagcat
    13381 gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc aaatggcttt
    13441 gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt aactgctctt
    13501 tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg atttggtttc
    13561 tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt tcctgtataa
    13621 aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa ctactgctgg
    13681 attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa taaattggta
    13741 ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc aattgaccga
    13801 cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc tgtttttgtc
    13861 gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag gctctctctg
    13921 tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct gaataatcat
    13981 gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt ggaaaatatg
    14041 cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt ttttcttgtt
    14101 ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt aattcaagtg
    14161 aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa agatagagac
    14221 ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg atccgtaaat
    14281 ggaattttat taaggaatga ttgagctgtt ttgtcagttt ttcgagatag gagggcgata
    14341 tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa ttgattagta
    14401 acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac ttagaaaaaa
    14461 cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta tgattagaat
    14521 aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt cggatcagtt
    14581 ctttgattgt ccgttttgga atatgtaagt cattggataa ttcttgtaaa gaacaattat
    14641 ttttttgaat gaccatttgt agaacaagta actctttttt tcttgcttca attagcataa
    14701 atacctcctt tttattctgt ttttgtatat agttagacaa aaactgtgag tggaaagtca
    14761 tttcagtctg tgttattctc acagagtgtc gccttctttt ttatataacg ttatttaatt
    14821 tttttgttac cttgtttatt ctattgttta tttcctaggg tattttgtca agatattctt
    14881 gtttttaagg cggaaaaatt gatttttaat ggatataact aaatatgttt tgataattat
    14941 caataatatt tttagaatta tttatgttat ttatttatgg gtagatgaaa agagacggag
    15001 gtaagaatga tggggagagt aacaaattat agtaaagaat atttgctgtt caaaagtatg
    15061 gtatacgtgg aagaatatgg aatgaagtca attactgcaa gagagttagc agagttctgc
    15121 ggttgttcta catacccgat ttatacacat ttcaaatcaa tcagcgggtt aaaagagaag
    15181 atattagaag aaatcactgt ttattttgaa aactacttag ctgaatgcag ttccgatgat
    15241 gttttaagca ttgtctactt attgaaagat tttttttcta tccatgaaag tattcgtgaa
    15301 atcattgcaa aatctgattt agacatggat tcgctattta agcggccatt tttctcatat
    15361 gtcaaaggtc acacgaagat caaagaagaa aagtcgcttg aattggtatg gcttaatgca
    15421 ttgggaagtt tgcgctcaga tgattcttct caagattttc ttgaaatgat gaacgctgtt
    15481 tgggataaaa acgaggtgac ggatgaatac ttggaaaata tcttaaagga acagtcacct
    15541 taggcagcaa catgaaaaag tagatcagcc acctttattt ttcgtaaaaa agattgtaaa
    15601 atgtctaacg aaacattgga atgaaggagg aagcgacaac gttaccacag caatttatca
    15661 aaaaatatcg tcttctttta ggcgaagaag catccgattt tttttctgcg cttgaacaag
    15721 gaagcgtgaa aaaaggattt cggtggaatc cactaaaacc ggcaggattg gacatggtcc
    15781 aaacttacca tagcgaagaa ttacaacccg ctccctacag caatgaggga tttcttggca
    15841 ctgtcaatgg aaagtctttc ctccatcaag ctgggtatga atatagccaa gaaccaagtg
    15901 caatgatcgt tggaacagct gctgcagcaa aacccggtga aaaagttttg gatttgtgtg
    15961 cggctcccgg tggtaaatca acccaattag ctgctcaaat gaaggggaaa gggttattag
    16021 tgacgaatga gatctttccg aaacgtgcga agattttatc agaaaatata gaacgatggg
    16081 gagtttcgaa cgcaatagtg acaaatcatg cacccgcaga acttgtccct catttttcgg
    16141 gcttttttga tcggatcgtg gttgatgctc cttgttcagg agaaggaatg ttccgaaaag
    16201 atccaaatgc aataaaagag tggacagaag aatctccgct atactgtcaa aaacgtcagc
    16261 aagagatttt gtcttcagca ataaaaatgc taaaaaataa aggacaactg atttattcga
    16321 cttgtacgtt tgcaccagaa gaaaatgaag agatcattag ctggcttgta gaaaattatc
    16381 cagtaacgat tgaggagatt cctttgactc aatccgttag ttcaggacgt tctgaatggg
    16441 gaagcgtagc tggtttggaa aaaacaatac gtatatggcc tcataaagat cagggtgaag
    16501 gacattttgt tgcaaagctg acttttcatg gacaaaatca gatgcataaa gaaaagaaga
    16561 cacgaaaaaa aagtaaagtg caaatgacaa aagagcagga aaaactctgg acggagtttt
    16621 caaatgattt tcattatgaa gctactggaa gacttttagt gttcaacgac catctttggg
    16681 aagttcctga gttagcccct tctctcgatg gtttaaaagt cgtacgtaca ggtttgcatt
    16741 taggagattt caaaaagaat cgatttgaac ctagctatgc attagcgctt gctacaaaaa
    16801 agatagagaa tataccttgc ttgccgatca ctcaaaagga atggcaatct tacacagcag
    16861 gagaaacatt tcaacgtgat gggaatcaag gatgggtttt gctagtatta gataagatac
    16921 ctgtcggatt tgggaaacaa gtaaaaggaa cagtaaagaa ctttttccct aaaggattga
    16981 gatttcactg agtggattag ttcgtttatt tttttgtcta tgctatgatg gagatagata
    17041 aaaaggagct gatacttatg aaaacatcaa caaaagtaac aatcggatta agcgtagcag
    17101 ccgctgccag tgtagcgaca gcagtcgttg tatcgggaaa agtaatggaa aagatccatc
    17161 atatgactac tcggacgaaa gtaaaaaaat tcgtctatga caaatttgat ggaaatgaaa
    17221 aattactaga aatcgtagat ggtctttcag atagtgactt ggattctttg atgggaatgt
    17281 tagcaaaaat caaatcagga aaaaagaaaa tctctgttta tggtgattca atcaaagatt
    17341 caaccgaaga tgtaaaagac cggctaatgc attttgttga aaaaatgatg taagagaaac
    17401 tagcttttga acactattca ttcgaaataa ggggttgtga caaaattatt gtcacaaccc
    17461 ctttaatcta tctttaatta tgttcatgaa gcaataaaag atagcaaaac agtaaatcgt
    17521 tggctttttt tagattgaaa ccagactgtt cttgaaattt ttcgattcga tattttagag
    17581 tattacgatg aagaaacagt gtttttgcag cagagctgac attgcctaat tcattccata
    17641 attcttgaat gatcacttcc atttcatttt gtttgatcag ttctatgtaa cttttgatca
    17701 acttactatc ttttacagta tcttttgtat agtaatgtaa agccacatct tgcatagaaa
    17761 aagtacggct gtgagtataa agattgtttt gctctgttaa aaaaattttt ctttcttcag
    17821 caaacaaagg gacgaggtcg gtgccaggtg aatggaaaga accaacgaat acagacgttg
    17881 tcgtatcaaa atcagcatct aaagatagaa aaataccgtt gatttcagta gcatcaagat
    17941 aatcacggtt tatttgttcg actaagagcg catctgtgtc tgtataaaaa aagaaatcag
    18001 cagaatagcg aaataattcc aaaatagtat cttgccattc tttttttaag aattctcctt
    18061 ttgattcgac gtgaagctgg atcaaacgaa agcttttttc ttgaacacaa gaagcatctt
    18121 taaaaagata gtgataccat gggtgttttt tttgatcgtt gattacaggg aataacgctt
    18181 tcaaaaggct ggtttcttgt tgagataatg aatcttgttt gatccagata aaagcatcat
    18241 caattgcaaa agaaaggata tccgcttctg atgctggaga gttttttttc tgtgcttgtg
    18301 ggtaaagagc aagtaattta tccattttca tcttgttcac ctcttgtatt tattttacca
    18361 tagaaaaaaa agttagttac agccttttta tcatatgcta aagtaaaaca agaaaaaaca
    18421 aaggcaggga agggtaattg gtaaatacaa atcttgatgc agtagaaaaa gaaaaatgga
    18481 tgcgccttgc attggcagaa gcaaagaaag cagaaatgct tcatgaagtt ccgattggag
    18541 cagtggttgt attggatgga aaagtgattg gaagaggata taaccttaga gaaaccacgc
    18601 aagatgctac tacacatgca gaaatgcttg caatcaagga agcctgtgaa aaagtgggaa
    18661 gctggcgctt ggaagatgct gcactttttg ttaccttaga gccttgtcct atgtgcagcg
    18721 gcgcgatgat tttgtctcgt gtagcagaag tttatttcgg cgcgtatgat ccaaaaggcg
    18781 gaacagccgg taccttgatg aatcttttgg aggacgagcg gtttaaccac caagcatatg
    18841 tagaaggtgg cattttagaa gacgaatgtg ggatgatttt gactgacttt ttccgaaagc
    18901 tccgccagcg aaaaaagaaa ttaaaaaaag actggtcaac gaatgaaaaa taaggtatac
    18961 tattctttgc cgaaaggcta ggacaatggt gggcttgtga agcgtgtcag gtctggaagg
    19021 aagcagcact aagcaggttc cgccatgtgt ctgattataa tgaaacgaca acagactttc
    19081 gggtctgttt tttttatttt tatagtactg taaaaatgaa tgttcctgat tttttagcca
    19141 gactgattta tcgaatattg tacttataca atttgcttca aggcaggaaa aactctgaaa
    19201 tagagcttgt ttcaatggaa gaaaaagctg cctatggtat gataaagata aagagaggcg
    19261 agagggtaca tggaaaaaat agtgatttta catacgaatg atctgcattc tcatttagaa
    19321 aattggccaa ggatcagaag gtttttggat caacgtaaac gagaaaacga aaagaaagaa
    19381 aaaacgacaa cgattgcagt agatctaggt gattttgtgg atcgttggca tccattgtca
    19441 gaagcaacga atggacagga aaatatcttg ctgatgaatg aagttggtta tgatgcagcg
    19501 acgataggaa acaatgaagg cgtaggaaat tccaaagaag agttgaatca tttatacgat
    19561 catgctgagt ttgatatcat cctggataac ttattcgata aaaatacgct ccagccaccg
    19621 gcatggacga aaccatataa aatcattaca acgaaacacc agacgaaaat cggactaatt
    19681 gcatttactg ctccatttcc gcttacttat aatccgaatg gctgggacat acggaatcct
    19741 tatgatattt tgccagaatt agtcgaagat ttacgttcac aagtggatgt tttagttttg
    19801 atgagtcatc tcgggattca ggatgatttc caaattgcaa aagaaatacc agcaattgat
    19861 gttattttag gatcgcacac ccatcattta ttcagagaag gtcacgtaat caatgatgtc
    19921 caaatagcag ccgctggaaa gtacgggcag tatgtcggtg aagtccattt aaccatcgat
    19981 gaagagaaaa aaatccttaa gcattcagcc aaagccatcc ctaccgaaac aatgacagct
    20041 tttacagaag atgaacaaga aataaatagt tatctagaac gtgggcatga attattaaaa
    20101 gaaaaagaag tagcagattt gccattttct ctgagtctag atatcttcaa tgagcattcg
    20161 tttatccaag tagcctttga ggcggtgaaa gaacgaggaa agacggaagc agcaatatta
    20221 aacagtggtc tgtttttaac tgaaattcca aaagggcggg tcaatcagga tcagcttcac
    20281 actgcgttgc cgcatccgat gcacttgtta aatgtcacat tgggtggaaa tgatctgatc
    20341 cgcttagttt tagaaataga aaaaaatcgt aactttttac gtaactatcc tatgaaggga
    20401 atgggatttc gcggtaagat ttttgggcag atggtctata atggaatcac atatgattct
    20461 attaaccatc aagtcttttg gaagaacaag ccaatcgatc tgaataaaaa ttatactttt
    20521 actaccgtag atcatttgat gtttgttcca ttttttccaa cgattgagat tgctggaaaa
    20581 aatgaatttt tgttccctga atttatccga agtgtggtag gagattactt gaaagctcac
    20641 tacccaatca aataaaaaca aggtataata aaaaagatag gtggtgaacg catgtctgca
    20701 aaacaaaatg aacagcagaa cgttgagcat aaaacagaaa ttgctaagaa aaaatcatca
    20761 gctgaacagg aaacaacagt caccgaagaa ctgacagctg ctttggaaga aatcgttgag
    20821 gagcctaaag cagaaaaagt caaaggcgag ctagtgacac tgttagatga cagtaatttt
    20881 ttgataggtg aaagacatta tcggcttgtg tccgattatc gtgaaggatt taatgcagaa
    20941 aagcttggag aacgttacag tgatgtacta gcccgatatg attatatcgt aggagattgg
    21001 ggttatgaac aactacgcct aaaaggattt ttccaagcgg ataatcgacg ggcgcatcca
    21061 gatcagcgaa tcgattcctt ggaagactat ctttacgaat attgcaattt tggttgtgcg
    21121 tattttgtca tcgagagaat cggcggtaaa aaagaaaaga caactcagcg ccgaaaaaag
    21181 aaaaaaaacc ataatcgtgc gtatatcgat gagaaaaaag gaccagtcgc atctaatcgc
    21241 aaaaaaccgg ttattaaaaa tcgaaaaaca gagtcagcaa aaacctctga aaaagtaaaa
    21301 aaacaagaac aacctaaaca agtagaaaaa acaaaaacag catttacgat cagaaaaagg
    21361 gaagagtagt ggatgaatgg actataaagg atatttaatt gatttagacg gaacgattta
    21421 tcgtgggaca caacccatac cggccggtaa acgatttgtg gaagaattgc aaaaaagaaa
    21481 attacctttc ttgtttgtta ccaataacac gactaagtca ccagaaacag tagctaatcg
    21541 tctagcagat gagtttgaca ttcatgtagc tccagagaca gtctacacag ctactttggc
    21601 aacaatcgat tttatgaaag cagatggaaa agggaaaaaa gtctatgtta tcggagaagc
    21661 aggattgatt gacttgattt tagctgccgg atttacatgg gaagaagagg ctcctgacta
    21721 tgtagtggta ggcttagata attatctgac ctatgaaaaa gtagttaaag caacacttgc
    21781 tattcaaaaa ggtgctactt tcattggaac gaacccagat aagaatatac ctactgaacg
    21841 cggccttctt cctggagcag ggtcagtcat ctcttttgta gagacagcaa cccaaacgcc
    21901 gccgatctac atcggaaaac cagaagccat cattatggat aaagccgttg aagtattagg
    21961 gctgcaaaaa gaagaagtaa tcatggttgg agataactat gaaacagata tccaagctgg
    22021 gatacgcaat aatatagata ctttgctcgt tttatcaggt tttacaaaag aagaagatgt
    22081 acctgggttg cctatcccag ctacctatgt gaaacaatca ctggatgaat ggagcttcta
    22141 atatgaaaaa ttcgagatgg cagtggatgg aatatgctgg attgttcagc ctatttctga
    22201 cactgatcag tctggcagtt ggtgtgacga tcaactttcg accactatac gtatttgata
    22261 tcggacattt gcaaattttg gattatactt ctttagatca agagacgtta ctgaaaaatt
    22321 ttgatcacct aatgaattat ctcaataatc ccttcaaaac catcctcagc ttgccagatt
    22381 tcccagtttc tgctagcgga gcccatcact tttatgaagt gaaaattttg tttttagtgg
    22441 attatgctgt gtttttcatc actttgatcc cgagtatttt gtttatcaaa tatttgcaaa
    22501 agaatgatcg gctttggcgg ttgattcgtc cgtttcaaat cggcatgtta ctgccagttg
    22561 ttttcggttt tttcatgatg ataggatttg atcgcttttt tatccttttt catgaaacgt
    22621 ttttcaacaa cgatgactgg ttattcgatc ccgttactga tccaatcatc aatgtacttc
    22681 cagaagaatt tttcatgcat agctttatct tattttttgt attattagaa ttattttttg
    22741 ctgtttttct tttcttagga aaaaatagct tgaaacaaac aaaaaagaaa gaactcgtct
    22801 aaatagacgt tttctttctt tttttgttga attattgagt aaggcgactt cttttttctg
    22861 gttctttgtg tgctaatcga ctggcagcag cagcggcgat cgctccgacg atatcatcta
    22921 agaaggtatg gacatgaacg ccatcgtggc tgtttagttc ttttaaaata ccaggtttca
    22981 ccttgtcgat ataaccataa ttcgtaaagc cgatcgtacc gtaaacattc acgattgaca
    23041 aggctaggat ctcatcgata ccgtaaaggc cttcgtcatc ttcgataata tgttgcaacg
    23101 gactcatcaa ttttttcttt tctgctaata tatctaattg gattccagtg ataatggcgt
    23161 tatgtacttc acgctttgtt agcacgctgt tgacactttc agtacaatcc gccaaagtca
    23221 aatcagaaat atagtctttt tgtaaaaaca tgacaagttg tgcgatatct tctacagtta
    23281 cgccacgttc cttcaatagt tctcttgcct tttcttctaa agtctcagtt ttgacgacca
    23341 tattattgct ccccctttat tttttctttc tctatttcat gttaacggaa catgaaaaaa
    23401 atgcaagaaa ctgctttcgg gtttaacttg aaatgcaaag agatataaaa aatcaactag
    23461 gacgaagatt tttatcgtat aagcttgtgc tgtgaccagg ctgagttcgt tcctttggat
    23521 taatatagtg taaagcgttg ttcactgcag taggtgcttc accgagccca gtagcaatca
    23581 gtttcacttt tccttcatag gtacagatgt caccagctgc ataaacgcct ggaatagaag
    23641 tagacatatc tgattttaca gtaatggcat ttctggtact atcaagtccc caagaagaaa
    23701 gatgttctaa attagaagag aatccataat tgacgatcag agaatcaatc atcaaatcaa
    23761 tcgtctcgtc acttttcact ttttttaacc ggatatctgt cagttcccca ttattgcctg
    23821 ataatccatc gatgagatag ggagtaagca agttgacaga tgaagatttc aagcgtgata
    23881 cactatgttc gtgcgcacga aattccgggc gacgatgaat caagtaaact tcgcttgcaa
    23941 taggctctaa catcaacgtc caatcgatag cagaatcacc acctccagca atagcgactt
    24001 tttttccagc atacttcatc aaatcattca cgaaataatc caatccgtgg ttttcaaatg
    24061 attcagcatt atctaaattc aattttcttg gctggaatga accatttcct aaagctaaaa
    24121 tgactgcttt ggaataatgg atgcctttat ttgtagtaat ctcgataact tcgtcttcac
    24181 gagtaagcga gagaacttct tcttttaaat ggaatgtatg gttaaaagta gttaattgtt
    24241 tttctaattg atcgattaat tcacttgctt ttattgctgg atacccagga atatcgtaaa
    24301 tatatttttc gggatacaaa gttgctaatt gaccaccaag ttgaggtaga ctgtcaatga
    24361 tttttgtttt agcttggcgc attcctgcgt aaaaagcggc aaacatccct acaggacctg
    24421 caccgatgat tgtaatgtca tagatttcca taaaaattcc tcccttttca cactatatca
    24481 gaatttaatt gagaatgctt ctcaaaaatg tttgaattcg aaatcgaaac ggtaagttat
    24541 agaatgtatc tagtgatgaa aaataaaaga aaacaaatct agtattttta cgagaggtta
    24601 ggtattatgt tattattatt atgaagtatg caaatattag gaggaaacaa tatggcattc
    24661 ccacaactag atttaaaaag tgaaaaagga ccaaaagctg ttatcaaaac aaaccgtggt
    24721 gacattactg ttcaattatt ccctgaacta gcaccgaaga cggttcaaaa tttcattgaa
    24781 ctttccaaaa aaggttatta tgatggcgtg atttttcacc gcgttattcc tgatttcatg
    24841 atccaaggcg gcgatccaac tggaacagga atgggtggcg aaagtattta tggcgaaagt
    24901 tttgaagatg aattcagtcg tgaactgttc aaccttcgcg gagctttatc tatggcaaat
    24961 tcaggtccaa acacaaacgg aagccaattc tttattgtaa ataacacaaa tgtgccagct
    25021 aatatgcttg gacaactaga aggtgctgga ttcccatcag aaatcattga agcgtataaa
    25081 ggtggcggaa ctccttggct agatttccga catacagtct ttggacatgt actagaaggg
    25141 atggacacag tagatgaaat cgcaaatgta caacgtggtc cacaagaccg acctgtacat
    25201 gatgtagtga ttgaaacaat tgaaatttca gaatagtttg gtatggtacg gagtaattat
    25261 gaaaaaggac gaaacctagt aaggtctcgt ccttttattt tgaataaatg atttaatcga
    25321 ttgtctctaa agcatacttg attcgattaa gtccttccag taaagtttct tctggacaag
    25381 caatattcaa gcgcatatgc ccatgaccgg aaggaccaaa cgtgatgcca ggatttaata
    25441 cgacttttcc tttccgaaca agagtgttct ccaattcttg gtcgtctgaa atataggcgg
    25501 taaaatctaa ccacattaag taagttcctt cagggttcat tattctcact ttcggaagat
    25561 actgttcaaa aaactgatta actgtttgaa catttttttc tagataagga atcagttggc
    25621 ttaaccattc tccacccgtc tcataagccg cttgggttcc aatcagtcca aaagtgttga
    25681 tttcgtgctg ttggttcatc actaaatgtt tttcaaaatc agctttcatc tctggatttt
    25741 tgataaatac cattgaattt ttgattgcag ctagattgaa agtcttggtt gcggaagtaa
    25801 aagcaactag tagatcatct aaattaggat caatcgttag catggaagta aatgtatgat
    25861 cgaataaagt taaatcttga tggatctcgt cactaaacag aatcacttga tgttttaaac
    25921 acaattcgct taactgtttc aattcttctt tgctccaaac acgccctcca ggattgtgag
    25981 gattgcataa aatcattgct tttacattgt tttcttcgat ttttttctcc atgtcagaca
    26041 agtccatgat gaaatgatta tttttttcta gcaaagagct tcgaacgatg tgacgttgat
    26101 ttgtttctat aatagaggaa aatggaggat aaactgggtc atgaatcaaa atagaatcgc
    26161 ctggttttgt aaaagtttgt actgctgcag ctaaactggt taacacacca cttgtaaaaa
    26221 cgatattttc tttcgtcaac tccacagcat ggtgttggcg ttcccaatga atgatagcct
    26281 gatatagttt atcagacaga gtggcataac caaagattcc atgtttgatg tattcactaa
    26341 acgctttagc taccccaccg ggagacaaga agtccatatc tgctacccac aaaggaagta
    26401 agtcatccat ttgatatgtc tcagcaattg cgtcccattt tacactttct gtatcttttc
    26461 ttgaaatctg tttatcaaat tcgatcatct taagacctcc agtcattttc gtcgcttctt
    26521 tttttgagct atttacagta taatgataaa tgcgaagaga gggaagtaga tgacttataa
    26581 aattggacaa ataattgaag gaaaagtgac agggatacag ccttatggcg cattcgtttc
    26641 tcttgataac gagacgcaag gacttattca tgtttcagaa gtacaatccg gttatacgaa
    26701 aaatatccat tcattgttaa aagtcggaca gccagtaaca gttcagatca tcgacattga
    26761 tgaatattcc aaaaaaatca gtttatcttt aagaacgtta gaaaaaacaa atccagctgt
    26821 tccatatcgc agaaaaaggt atttcacgaa taaaaatcgt aagatcggat ttaccactat
    26881 tgcggagcag ttacctattt ggatagaaga agcattatct gaactagaac ggaaagaaaa
    26941 taagaaataa aaagtgtatt ttttctgaaa atgtgctaga atttttatgt aagccataga
    27001 acaagttgat cgaggtggaa attttggcaa gcaaaagagt tgctggtaca attatgttgc
    27061 atttggagga tggtactaaa aaatttttgg tccattcaat tgatgacaag ttggaatttg
    27121 cgttagcgga actttctgaa ggaaagactg gtttagcgaa tatacttaat ttcttaaaag
    27181 aaatagttca tatcgatgtt agcaagatta gtttgatgga attgacaaat acacatatta
    27241 aagaggaaaa tgttccgttg tttgtctttg aaactgaaca aaagaatcaa agagaagaat
    27301 taggcgaagg atatatttgg gaagaaccag gtcaaatgcg ctctgttctt ggaacttacg
    27361 aagtagaggg agtgcctttc ttctaaaata cagattaagc gaacaaagaa gtttgaattt
    27421 ttttcaaact tcttttttta ttgtttggtc caaaaaatac aatgaaatgt tctgctaaag
    27481 atagtttttt agtgcatttt attttcaaaa tatgttgaaa acggttgtat aacgaataat
    27541 aataattgat cgaaaaatca tttcacaaag taatgcttaa tttatagttg accaaggaag
    27601 aaatacatga taaatttaac agcgttgatt ctgaagtgga tgttatatca catttctaaa
    27661 aaatttaaaa a
//
LOCUS       DAYYKN010000024        27510 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000024 DAYYKN010000000
VERSION     DAYYKN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.024"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..964)
                     /locus_tag="ACHBB7_000883"
     CDS             complement(20..964)
                     /locus_tag="ACHBB7_000883"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM9208851.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1260..2204)
                     /locus_tag="ACHBB7_000884"
     CDS             complement(1260..2204)
                     /locus_tag="ACHBB7_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9208852.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2425..3639
                     /locus_tag="ACHBB7_000885"
     CDS             2425..3639
                     /locus_tag="ACHBB7_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9208853.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3661..5676
                     /locus_tag="ACHBB7_000886"
     CDS             3661..5676
                     /locus_tag="ACHBB7_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM9208854.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5759..7132)
                     /locus_tag="ACHBB7_000887"
     CDS             complement(5759..7132)
                     /locus_tag="ACHBB7_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9208855.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7158..7442)
                     /locus_tag="ACHBB7_000888"
     CDS             complement(7158..7442)
                     /locus_tag="ACHBB7_000888"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9208856.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7444..9459)
                     /locus_tag="ACHBB7_000889"
     CDS             complement(7444..9459)
                     /locus_tag="ACHBB7_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9208857.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9638..9943)
                     /locus_tag="ACHBB7_000890"
     CDS             complement(9638..9943)
                     /locus_tag="ACHBB7_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM9208858.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10032..11063)
                     /locus_tag="ACHBB7_000891"
     CDS             complement(10032..11063)
                     /locus_tag="ACHBB7_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9208859.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11270..12145
                     /locus_tag="ACHBB7_000892"
     CDS             11270..12145
                     /locus_tag="ACHBB7_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9208860.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12297..13139)
                     /locus_tag="ACHBB7_000893"
     CDS             complement(12297..13139)
                     /locus_tag="ACHBB7_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9208861.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13231..15108)
                     /locus_tag="ACHBB7_000894"
     CDS             complement(13231..15108)
                     /locus_tag="ACHBB7_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9208862.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15105..16526)
                     /locus_tag="ACHBB7_000895"
     CDS             complement(15105..16526)
                     /locus_tag="ACHBB7_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9208863.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16529..19918)
                     /locus_tag="ACHBB7_000896"
     CDS             complement(16529..19918)
                     /locus_tag="ACHBB7_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9208864.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20175..21566
                     /locus_tag="ACHBB7_000897"
     CDS             20175..21566
                     /locus_tag="ACHBB7_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9208865.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21639..22286)
                     /locus_tag="ACHBB7_000898"
     CDS             complement(21639..22286)
                     /locus_tag="ACHBB7_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9208866.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22294..22980)
                     /locus_tag="ACHBB7_000899"
     CDS             complement(22294..22980)
                     /locus_tag="ACHBB7_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9208867.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(22986..24059)
                     /locus_tag="ACHBB7_000900"
     CDS             complement(22986..24059)
                     /locus_tag="ACHBB7_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9208868.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24281..24955
                     /locus_tag="ACHBB7_000901"
     CDS             24281..24955
                     /locus_tag="ACHBB7_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9208869.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            24960..26300
                     /locus_tag="ACHBB7_000902"
     CDS             24960..26300
                     /locus_tag="ACHBB7_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9208870.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26449..27435)
                     /locus_tag="ACHBB7_000903"
     CDS             complement(26449..27435)
                     /locus_tag="ACHBB7_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM9208871.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8008 a         5211 c         5005 g         9286 t
ORIGIN      
        1 agttcatatt cattgtttct tataatacag tgttcaatac ttctcttaaa gcacttgttg
       61 atttgctgaa cattgctttt tcttcttcgt tgattgataa ttcaacaact tcacgaacac
      121 cattttcgtc aacgattgat ggcacacctg taaagatatc tttttcgtcg tattcaccag
      181 ttaaataagc agatacaggc aatacagctt gttcgttatt caagatagct tttacgatac
      241 gtgcagtact cataccaata ccatagtaag ttgctttttt acgatcaatg atctcataag
      301 cagcattacg gactttatca gcaatcacat ctaattcatc ttgggcaata cgatggtctt
      361 tttctacaat ttcaaacact ggtttaccac cgacagttgt atgtgaccat gcagcaactt
      421 ctgaatcacc atgttcacct aagatatagc cgtggacgct gcgtgggtca acttttaatt
      481 tcaatgcgat ttctttacgg aaacgagttg tatccaatgt tgttccagta ccgataacac
      541 gtgatgttgg cagaccagat tcgttccaag cgatatatgt caaaatatct actgggtttg
      601 aagcaacaac gataataccg tcgaatcctg atcccatgat ttcttttacg atttggcgca
      661 tgattgacgc atttgtttta actaaatcta aacgagattg accaggtttt tggttgatac
      721 cagctgtgat aacaacgata tcagcatctt tacattcttc atagccacct gaccatacag
      781 ccacattttc atcgccccaa gccataccat ctaaaaggtc caatgcttcc ccttctgctt
      841 tttcttgatt cacatcgatc aatactaatt cattagagat tccttggttg atcattgcat
      901 aagcgatact tgttccaaca aaacctgtac cgacgattac tactttacga cttgtttttt
      961 tcatgaataa acagctcctt ttcataacta tttgttactt tcatgaaaag tatagtcttg
     1021 tttattcatt ttatcaacat gttaattata atagttgtta ttataatttt tgatggaaac
     1081 gaattcctga atacgcataa cagataaatg acaaatgttt acacctatat cccctgataa
     1141 tacaaccaaa agtttagaac tgaaaataaa tccaaaaaaa ttcgctgcaa cgatataata
     1201 agacagcaat cgcacctaaa acaattttcc ttcatatgct ttagtgaaag attttcttct
     1261 taatgtttat ttctccattc attgggagta catgttgaat acttcttgaa taatttgaat
     1321 aaagaagtcg tttgtttata tccgatacga ttagcgattt cttccaacga aaaactcgtt
     1381 tcttgaatca gttccaacgc aaagtcgaat tttttcttta gaaccagttc ttgaaaggaa
     1441 gcatcaaaat acattttcaa tttattacta agatagaccg gattgtagcc aaaatgagct
     1501 gctgtttctt ttaacgtaca agaagtaaaa tgcttatcga tataatctaa tactctaagc
     1561 atctcatttt ctgcgcatcc atctgctgat gaaaagcttt gttgttctaa taaatgagtc
     1621 aattcgatca gtaatagtga tgtatagaga tttaacgaac ggatcttatg actgtatgta
     1681 tcaaaataaa tcaacagcat attttcaatc aaatgcgtca aacgacttgt ttttccgccg
     1741 cgaaaatgaa tgaattgtgt atgttcccct ttttcttttg atgcatttat caaaaaagaa
     1801 gaaagtagat tatttgtgct tgccaaatga ttcaatatct ctgtagataa ggtttcttct
     1861 tttaataaga tgttgattaa aatatcctct tgccctgtgt attcgattct ttgagtagct
     1921 tctttatcaa tgatcaccac gtcactttct tccatctcta tcctttctcc attaataatt
     1981 tgtaaacatg taccgctata catataattg atttcaacaa aatcatgtgt atgctcggga
     2041 acttccgcat atcgacggtg tttactgata tgaatatttc cctgttgaaa tagatcccct
     2101 cggtggattt ttggcggtgt ctgaagatgg taagctaagt ttaaatcatt gatattcatt
     2161 ccttgttcac gctgcagagt ttcgattgat tcttcgttac gcaaaaaaaa tgtctaattc
     2221 agctagttcc aattgactcc ctcctttttg taagcggttt ctttcgctta ttagttcacg
     2281 ctgtactgct attatatgca aagctatttt tggtttcata aagactttct atggttggga
     2341 catgaacttc ctgtgattcc gctattgaga gaataaccac ttcatactat atttttgata
     2401 agcgcttaca aaaatgaaag gaacatgctt atgaataaaa agatgatgaa attttctctg
     2461 ctgtcagtct ctcttatttt gacttctgct ggtgcagtca cgtctgcaat tcccagtatg
     2521 tcagctactt atgccacgcg ttctttatct gctgttgaat tattaacgac tgctccctct
     2581 tttatggtca ctatttttgt tttgattagt agttttattg ctaggaaaat cggacaaaaa
     2641 caaactgtga tcatcggatt agtgattgca tcgatttgtg gtatcctccc cctcttctca
     2701 caaagttaca gtattgtctt agcttcaaga gctggactag gtattggctt tggattgatc
     2761 aattctttgg cagtcagcat gattgctgcc ttttatcacg gagatgaacg agcctctatg
     2821 attggttttc aaagtgcatt tcaaggattt ggtgcagctt taatgacatt tgttgcagga
     2881 cagctagtac agttcggttg gcaatatacc tttttagtct acgccatcag cttacctatt
     2941 cttgtcttgt ttgttttgtt tgttcctaca cctgaaacaa ctgaacttca atcgcatggc
     3001 acagccccaa caagacaagt aatcaaaacg aatcctaaaa tgattagtta tggtattttt
     3061 ctcttgctgg tcatcgtggt atataatgct gtatcgatta aattagccac agtcctgacg
     3121 acttattctc taggaaacga aactaatgca gcgaatatct taacaatcat gcaattggca
     3181 agtatggctg ccggagtttg ttttggctgg cttcagacaa aagcacatcg ctatatgctt
     3241 acactttctt tactactgat gggaatcggc tttgtattga ttgccactgt gataaacctg
     3301 tatgtgattg ggtttggtgc gatcctaaca ggaatttctt tttcgttatt cattccttac
     3361 ttattcaacc aagtaagtat ccaagcgccc aagtacgcgg agagtttcaa tacttcaatc
     3421 ttattggttg gcgctaattt aggtactttc attgcaccat atggtttgct ctttctctct
     3481 tttgcttctc ttgggagtaa aacacttgct gttttcgtca atggcagtat cttgctgttg
     3541 attagcgcgc ttattagcct tctagtagta cacaggacga atagaaagac agtgataaga
     3601 gtagatgaag aaataaaaga acaattgaca acaaaataaa tttgatggag gtcgtgtaaa
     3661 atgacaaatg aaaaacataa agatagatgg ctttggtatc ctggagactt tgagatccgc
     3721 catggactgc tacaaaattt ccaacgggaa gaacgaggtt ttgactggcc agcgtactgg
     3781 tacatggatg attgccatcg gaacgtcaag tttaagagat attactttct tgatcaacct
     3841 tccatgttta aagtgacgat ccaaggagtt gggtatgtag aaattaatgg acaaaaacat
     3901 ccatgtggaa aatggctgac ttgtccagct ggtaaagcaa aaattcgtat ctttgtagga
     3961 catacatcag ggctaccagc gatgtttatc gagggagacg aagtaaaatc agatattggc
     4021 tggacagcaa gtaattttat tgaagaatat ccagctggct ggagtccgct ttatgtcgat
     4081 atcgcaaaag atccaaatca aatctattat caaaaagaat gcgtccatcc agtcactgaa
     4141 acggagattg gcggaggaat cttattcgat tttgggcgag cagtgaatgg ctgtgtagaa
     4201 ctaatatctc tggatgacca tatcgaatct atcactctat gttatggaga gtccgatgta
     4261 gaagctatgg atatcgatta ttgctactat aagcaaatag gaatgacaag aaaaatgaaa
     4321 gctcgtaaac gcgcttttcg ctatttgttt ataccagagg tttctactgg aatggttcaa
     4381 cttcaagttt ttcatgaatt tttatctctt tcgaaacaag ctgctttttc aagcgattct
     4441 tctttactga atcagatctg gtcagtatca gaagaagcct tcaagttatg tagtgatttg
     4501 ttttttatcg acggtatcaa acgagatcgc tggatttggt ctggagatgc gtatcaatca
     4561 ttcatgatca atcaatatct tttttttgac gaggaaatca ataaacgaac gatattagca
     4621 ttaagaggac aaaatgaaat caagcagcat atgaatacta tcgttgacta ttcgatcttg
     4681 tggttgattg gtatcgaaaa ccactatatg atgacgaaag actatgaatt tttacgcatc
     4741 gtctacccta agatgcaatc attaatgaga tacttgatgg agcaaacgaa tgaattaggg
     4801 tttatttatg ggagagcaaa agattggata tttattgatt ggtctgaaat ggataaagaa
     4861 ggaacgcttg ctgcagaaca agtcttatta ctgaaagcct atcattccat gatcacttgc
     4921 ggagatatct tagctgaaac atatggagag aacgaaaatt atcatactca tctctatcaa
     4981 gaaaaatatc aactattgaa acaaaaccta tttgactact tttgggatga agaacttggc
     5041 gcttttatcg attgctatga atctgggaga agaaatgtga cgcgtcatgc gaatattttc
     5101 gctgttttat tcgattttgc taacaaagac caacagcaat cgatcttaaa gaatgtctta
     5161 ttgaatgaca ctatcacaca gattactact ccatatttca aattctatga acaagatgct
     5221 ttatgcaaac tcggacagac gaatatcgtc taccaaacga ttcttgatta ttggggaggg
     5281 atgttatctc atgatgcggt aactttctgg gaagaatacg accctactca aacaggaaat
     5341 gaaaagtaca gtatgtatgg agatccttat ggaaaaagct tatgccatgc atggggagca
     5401 agcccaatct atcttttagg acgctatttt atcggtttgt atccaacaaa gccaggttat
     5461 gaagaattta tgatacaacc taatctagat tcatttgaaa cactgaattg tacgctacct
     5521 atcaaagatg gaaagatttc gcttgaaaaa acaaaagata agatcgtgat tacttctgat
     5581 agaacaggcg gaacagtgaa aatgaatggt aaaagttatc ctcttctagc aaaacagcca
     5641 cttgtcttaa caagctattc aaaaataaat gcataaaaca aaaaactgct tgcgacatat
     5701 cagaagactt taaatcttct tctgatattg tccaagcaat ttttattttg aagtttgctt
     5761 aattctgttc tttagaaaca atgtttgttc tttttccggc aaaaagaaag ctagataata
     5821 cgacaagtaa aaagaaagcg atgattccaa ctgtcaaggc tatttgaccg tattctccta
     5881 gtttcccaag tacaagtcct ggtactagat aatcagtatc ggcaaatgta gaagaagcga
     5941 ttttaagatc tcctaatact ggaaggataa ataatggcag ccaactaatc aataagctat
     6001 tgaaagcaga acctaatacc gcccctcttc taccgccagt cgagttgccg aagacaccag
     6061 aagcagctcc gcagaagaaa tgtcctacta ccccaggaat gataactgta gtttgcaagc
     6121 ctatcatagc aaacatggat agtgttccta caaagaagct aacaaaaaag ccaattaata
     6181 cagcattagg tgcatatgga aaaacgatag gaacatctaa tgctggttta gaatcgggta
     6241 cgagtttttt agcaatccct tggaatgctg gaacgatttc tgccaacacc attcgaaccc
     6301 cttgaaggac gataacaaac ccagctgtga atgttccagc ttggatcagt gcaaagatta
     6361 cagcgttgtc tccattgctt agttctcttt caacaaatcc aggaccagct aacaaagcaa
     6421 gtgcgacata tacgatcgac atcaagagca tgatcgatac agtagaatct cgcaaaaagc
     6481 tgaaactttt aggaatcttt atgtcctctg tagaacggtc tttgtttcca aaaaactttc
     6541 cgatccatcc actagaagca taaccgatat ttccggtatg ccctaacgca acttgattgt
     6601 ttccagtgat gttcttcata aacggttgac aaagagcagg cgtaatagtt aatagtgttc
     6661 cttcgaagac cccgcctaat aagatcatcc acaaatttgc agtaaacccc gcgcttacta
     6721 aaatgactgc tgttagacaa gaaacataaa gcattgcttg acctgtaaga aaaatgaatt
     6781 tatatcttgt gaatctagca agtaaaatat tcacgatcat ccctactagc atgatcaaag
     6841 cagtaggtgt accgtattgt tgcaaagcaa tcgccactac tgcttcattg ctaggaacta
     6901 ctccttgagt atgcagagct gtttggaaca tcgaagcaaa aggggttagt gaattactga
     6961 ttaaaccagc accgccgatc agtactagga atccgacaaa tgttttgata gttccttgga
     7021 tcacttctga aaatttcttc ttctgaagaa cgagaccaag cagtgcaatc aaagaaacga
     7081 ggatggaggg ctgactgacg attgacacaa acaaattcaa caatttatcc attgatagtt
     7141 cctcccttga atcattttta ttcttttgac aagatcttcg ttactttttc ttttacttct
     7201 tcgctgtcaa tgatcgattg caaaggtacg agcttttcat caggtataga agttagcgta
     7261 tctttcaaag aatgctcaat gaagaaataa tcagccatat cgctagatac acttccaaca
     7321 tccgtatgac ttacttcaaa ctcgtccaca tctgctccaa gatctgcaag tgcttgtttg
     7381 atgttcatct ctaccataaa acttgttcct aatccacttt gacatactgc aaaaattttc
     7441 atatcattcg tctccttttc ttatgatcgc taatatttct tcttttgttg atgcgtgtaa
     7501 taaatcttga agattttgtt tattagaaag tagctttgtt aaatttgcca aagcccgaag
     7561 atgtgcctca ttatcgatag cagctaaaca gatgaacaaa ctaatgggat gggctggatt
     7621 atcaagcaga tccacaggtt gttgaagttt cagcaaagac atccctactt cgtttacgcc
     7681 atcttctgga cgagcatgag gtaatgcgat gtgatcgcca atatggataa acgcaccata
     7741 ctgctccacg cgctcgatca tagctttgat ataactttct tctattttat tttctgataa
     7801 tagcggctgt gcggcaaatg tgattgcttc ttgccagttt tttggttgat ccgataattg
     7861 gatcatttct tctgttaata attcagatag cataggacgt tgatcctcct tcttttctaa
     7921 aagtttgttc attttacgat ttaatacttg atagagcttt tcttcgtcta cgccttcttt
     7981 taattcgata taaggcagta gcgccgacaa caattcttgg gcagaaggca ttgaaaatcc
     8041 tggtatcagc caatctcttt gtacttgatt gatcagctga tttttttcca agtcagtcat
     8101 gattggatgc agaacataga gagacttttt cttatcagta agtgtgagag gtacagtaga
     8161 aaaaataata tcgtaagaag tttcgtcaac ctcgtgtaag cggtcagctg aattagtctc
     8221 tgtaaaaaag atcatcggga ataattgctg aagttcactt tttaagatca atgaagagct
     8281 aatgccactt ggacagacga tcacggctct tatttttcta tttctttctt ctgcgtccgc
     8341 ttttctgatc tctccgccaa atagtatagt gaaaaaacca atctcttctt cagggatgct
     8401 cttccctatt cgtctttcta acggaagtaa tgcttttctt gtcatttcgt agatggaagc
     8461 atattgatgc ttaatttgat cgatcatcac attaggtaaa taaaaagaat aggaaatacg
     8521 aaaataggca gggaccaagt gataaaagac attcatcaat aattcacgaa acgaagcaaa
     8581 ttctacggcc atcaggaatt ccatgcgatg gatgacttct gaagaacatt ccagcaaaaa
     8641 atcaagtgca ggctcctgga catctccttc aacgatcgat gctaagcaaa gagagaaaaa
     8701 tataatctcc gaattttgat gttcggaata gccaaggcca gtcaatactt gtcgactaat
     8761 gtcagtgact gcaagagaag ccaacagttc agcatctttt accagttcca tggatgtttt
     8821 tccaattcga atggttgtca aactcaaata ataagctaat tcatccattc gatttggtgc
     8881 gaatcgaaga tctgatagtg aaatcgtttt atctagcaaa tctctaactt tggcgtaata
     8941 atcaatggaa agggttgcgg ttaaatacgt gagcccaaat cttccagcaa gtgactgcat
     9001 caaacgagca gtgcgatttt ttgcttctct tcttaatatt gcttctgacc ctagcagcct
     9061 aaaaccagtt ttcctagtat acgacagctg acaatcttcc acttctaatt catttcttat
     9121 ctttttgata tctgacagga ctgtcccttt agaaacctga agaaaatctt gaaaatgaaa
     9181 aacagaaaca gacgttgcag tcgaataaac catcaaataa agaagatctc ttcgctcttg
     9241 ttcggtatac acagcttcaa atcgagtaat atccaaaaat ttttctgccc agtcgattgc
     9301 tcggaatttc tttatacgga tacgggtatc atccacatat tcggcaaaag gagttaattg
     9361 tttgatcgcc tcgcttgtat ttacttcgat gatctctgct gctttttcta gactgatttc
     9421 tcttggtgtt ttcaggagat aactaatttt gtagttcaca gcgctgttca cctcctactt
     9481 atattctagt atggtagcgc ttttaaatcg aggggtgttt ggtacaactt acagttggat
     9541 tttttttacg actgtaccga acgatttcta tcgaatggat acagaaaaaa agcaggtgac
     9601 caaatgtctc ctgcttttat gaattgattt cgtccgatca atgatgatca tgtttatttt
     9661 ttgaggtcag ttcttcaaat ttttctttga catcttcaac cgctttttcg atatcttttt
     9721 caaagtgatt gggctcatta ggctttgttt cctcttctac agaatttgaa ccagataaag
     9781 ctgtttcttt attttctgtt tcaccagagt ttgtgccagc taatgattct tctttgtctt
     9841 ctgtttcacc agaattagtt cctgctaaag ccacttcttc ttctgtttta ggggccattg
     9901 aacctcttaa tcgtttgtgt ttttcaccgt cataatttgt catgttggtt cctcctagta
     9961 gttctatact ttttcatcgt acaataagac agtttatgcg tcaaaaaaca aacccgattc
    10021 ttacaaatat gtcatatttt tccttctgat tcttgaatgc ttttgattcc ttcttccaaa
    10081 attttcattt gtaagattgt ttcttcatct ttgatgactt tttctgcgcc attaactaat
    10141 gtctcgtaaa tcccttcgta gacacggctg taatcaccaa cctcggaaat tactttttct
    10201 tcatgataac tcccttgttc atctatatag gtcaatacac cataatgttc gggcaaatcg
    10261 ataccaaagt cttcatgatc aggcatgtaa aacattttca gatgttcttc ttgtcgatct
    10321 tttgtttgtt tcacgaacat cccttttttt ccgtaaacga cgaaacttgg acgttctttg
    10381 atgcggaaat aactggattt tatcgaaatt ttcatatttc catagaaaaa atccagatcg
    10441 aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct gatatcatct
    10501 ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc atataaatag
    10561 ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa ataatcataa
    10621 tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt ttggactgtc
    10681 aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc tttttctttt
    10741 gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt ctctactagt
    10801 acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg actaggtgta
    10861 gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg atcagtatat
    10921 tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt tttgatattg
    10981 attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact ctttccattg
    11041 ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt tcagtatatc
    11101 ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc agaaaaacac
    11161 ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat caaaaagata
    11221 gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca tgcttattga
    11281 agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg tcgattacat
    11341 ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa aagctacgta
    11401 tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg gatttaaaga
    11461 attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca atcaaacgat
    11521 tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa agaaaatggc
    11581 ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg aagatttaga
    11641 tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa aaggtgattc
    11701 tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt ttttgatcat
    11761 ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg aggattgcgc
    11821 catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga cctatttgaa
    11881 gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa tggccaaaca
    11941 ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag tagcgacttt
    12001 ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga tatattctca
    12061 aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc aaaaaggcct
    12121 tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag aaagatagtg
    12181 tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt tttcaaacct
    12241 cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag gaatagctaa
    12301 cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag gaaaatcagt
    12361 atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac tttctgtact
    12421 tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag taagcgatga
    12481 ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc tttttcgatt
    12541 ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg ataagcgagt
    12601 gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg taagtctgtg
    12661 aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt attttctccc
    12721 cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag cacagtattc
    12781 gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat accgatcgta
    12841 tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt aaagggatct
    12901 gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat gttttcttgg
    12961 attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg agagatgatc
    13021 tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag tatccctatt
    13081 cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt tggtttcatt
    13141 tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt gcgtaatccg
    13201 ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc gtctagcaaa
    13261 gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat agattccctt
    13321 accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag gagaaatcaa
    13381 ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt ttgttaatgg
    13441 aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt ttagacctgt
    13501 cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt tcgccgatac
    13561 ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt cactatcttg
    13621 atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt caccatctac
    13681 tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt gatcatttgt
    13741 atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg ctttttcgtt
    13801 caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag gaatataatt
    13861 cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat ttactgggtc
    13921 gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat cattatcaaa
    13981 tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt tttgcgttcc
    14041 ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa ttttttcacc
    14101 gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag acgtatcatt
    14161 tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag gcgtgattgt
    14221 ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat tatattttcc
    14281 aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg caaagtcatt
    14341 gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt gatcagttgt
    14401 ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg gtgttccttc
    14461 ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag taccgatttt
    14521 tgtaactttc aacgttccat cattaccgac tgtattttta gggtagagat ggatagtatc
    14581 tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct catatacagg
    14641 gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt ctttgatcgt
    14701 gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat ttccatcagc
    14761 atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg cttgttttgc
    14821 tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga cgttataaac
    14881 tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt ctttcccgct
    14941 gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga tgacttgagc
    15001 tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta atggtaaaat
    15061 aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt tttcactcct
    15121 gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc aacaacgcct
    15181 aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt ttctttttta
    15241 ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt tgaaatggta
    15301 aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc ttttaatcga
    15361 taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga aatcaggtga
    15421 agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg gaggtaattt
    15481 cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg tttatttgtc
    15541 tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt actgacacca
    15601 aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc tgtactgctt
    15661 agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa cgaagggatc
    15721 gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc tgtcagcttt
    15781 tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc atccgcacta
    15841 tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt atgcaaggaa
    15901 taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac cttctcaaaa
    15961 ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt tgaaagtttt
    16021 tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt ttctttgacg
    16081 acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc ttgatcttta
    16141 gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt tgttgcttct
    16201 gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc ttcttctacc
    16261 gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac ttggaatgtg
    16321 acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc atttggatga
    16381 tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg aaccatttct
    16441 gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa gaagagacac
    16501 atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt ttcaaaaaaa
    16561 gataccaaat agaaaacgca caaccaatca tccctactag atagattcct aaacggccaa
    16621 ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa gaaatctggt
    16681 tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact tgaattgacc
    16741 catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct tctggtgtaa
    16801 aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag gtatctgttt
    16861 cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg aattttgctc
    16921 cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac gcttttagtc
    16981 gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt cccgtgtaaa
    17041 tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta tcatatccgg
    17101 caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca aaactggcaa
    17161 gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt gcttcttttt
    17221 tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct tgcatcgcat
    17281 acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac tggatcgtat
    17341 catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa gcgatcttga
    17401 tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt tctgtcagcg
    17461 tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt ccgtcgccat
    17521 gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg ccaataagat
    17581 ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg aattgtttag
    17641 tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca ggtaattctt
    17701 tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt gataatatct
    17761 cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt tcccacgtat
    17821 ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa gctgcagcat
    17881 ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca gctagattgt
    17941 tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct ttagcagatg
    18001 gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt gtcctgccgt
    18061 tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt ctcacttggt
    18121 aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg atggtatttc
    18181 cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc ttgactgaaa
    18241 gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt ccattgctta
    18301 cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct gttgcatgat
    18361 cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt cgcatacgag
    18421 actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg atgccaagac
    18481 catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt gcgataaacg
    18541 tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt cttgagatta
    18601 gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa taattgctgc
    18661 ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca tccgtcagca
    18721 aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca tctcttagtg
    18781 ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt acttgatttt
    18841 tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat ccttcgcttg
    18901 agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct aaagtatcga
    18961 caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc atacttcctg
    19021 accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca tttccacgag
    19081 cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac tttcgtatcg
    19141 catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc cgatcattag
    19201 gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta ttgccttgat
    19261 gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa gtgccctgat
    19321 catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca gctggcaagg
    19381 ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat gattcttctt
    19441 ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct gtctcatcaa
    19501 taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg taaagtgctg
    19561 gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt tccaacgtaa
    19621 ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc acatcactta
    19681 agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg ttattcgcat
    19741 ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca gctgaaattg
    19801 tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc acaaagtttg
    19861 ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg gtcgtcattt
    19921 ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat taaaataaac
    19981 aggcgttaca taattttatt tattttaata tgataattat tatacgaagg gcgctaaagg
    20041 gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc ttcgattaat
    20101 gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg ttgttggaat
    20161 taaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac aagtggatga
    20221 ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta aggtatatcg
    20281 aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa aaattggggt
    20341 atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa atgtcgcaaa
    20401 gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa acgattgagt
    20461 attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg aaacaatgca
    20521 atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt cagttaacta
    20581 gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta agttactacg
    20641 gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt atggtcaaga
    20701 ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct ttctttatta
    20761 tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa tggaaagctg
    20821 gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc ttgaaaaatc
    20881 tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt gtcaatacga
    20941 ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata cgaaaaaata
    21001 tgtcctactg ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa tatgttgttc
    21061 aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg actcatttga
    21121 cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt ctatatgctg
    21181 gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg atttattatt
    21241 tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat tatacttctg
    21301 ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat attgaagaat
    21361 atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct attactgaag
    21421 atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat caacccattt
    21481 tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca aatgaagaag
    21541 aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata aaccaccctg
    21601 ataaaaattt caggacaaat acacatagct gtttcttttt attgtattcc ttcagcaatc
    21661 caacgagatt tatagcgaaa gtcttttaac aactcttctt ttgtccaatt atttgcaatg
    21721 agatccgtca aagtgttcat tacgagggtc atggaaaagt ataggaaact aactgcttct
    21781 tccgacgatt gaatggagaa atgattgatt cttaataatt ccagccattt ttcgttcatt
    21841 ggagaatcag ccgatagttc tctacgtttg gttgtctgct gatcatttcg ctgggttaac
    21901 aagtataacc tttgccaata gatactttcg tgatctaagt cgcagcacca ctctaaatat
    21961 ttttcgatcc ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa gatatcttga
    22021 tggactatgt gagaactgct tttgatcgta tacaaataag catcttccaa atcttgaaaa
    22081 tatttataaa aagcaccgcg cgacatcccc atcttgtcaa cgatttctga gactttgact
    22141 tggctgatgt gctgtgagca aaatatctcc agtaataggt catcgattct ctgtttcttt
    22201 gcgttcgata aattgaaaaa agtttctttt ggcatccaca tctctccttt atctctgatt
    22261 atacgtggat agcccttcat tgtcatgaag gattcattcc ttctttttct cttcttctgt
    22321 caagtaccca tcttccattc gataaacgcg atcactccat tgaatcatac ggacatcatg
    22381 agtaaccata atagtcgctt tttgtttctc atgagcttct ttaactagta atttgacgac
    22441 ttcatatgca tgatctgtat ccaaactagc tgttggttcg tctgctaaga tcaagcttgg
    22501 ctcattatat aaagcgcgag caattgccac tctctgtctt tctccaccag aaaggtcttt
    22561 aggataacta tttttaagtg actggatatc taaagattgc agcaaggaat cgatttttgt
    22621 tgaatcttct ttttttgaag taacttggtc aactagttca aattgttcct ttaccgttaa
    22681 aaaaggaatc aaattagagc tttggagaat aaatcctatt tctttaaagc gtaaatctgc
    22741 tcgttttttt tctggaagct cagttagtga gtgaccgttg atcactattt ctccggaagt
    22801 cggtgtttgc aatcctcctg aaatggtaag gaaagtactt ttacctgaac cagaaggacc
    22861 aatgatgctg acgaattctc cttcctttac ataaaaatca attgctttca aagctgagac
    22921 ctttgtatga ccagaaccaa atgttttggt aacttttttc atcgttaata tttttttcat
    22981 ttttattaac ctcctattgc tgtaattggg tctactttgg ttatcgtacg tatcgaaaac
    23041 aatccgccta gcattgcgac aatgatcaaa atcccactat atagcaacca ttgtgaccaa
    23101 aaaacagcaa aaggcatcgc gcttggtaaa atcagactag tcaaataagc aaacaaaaga
    23161 gcaagcagaa cgcctaccac cccaacaata aaggactgtg ctactaaaga tttagcgatg
    23221 aagaaatttt tgatcccttg tgctttcatt actccaaaaa tagcagtctt ctgaagtgtc
    23281 atcacgtaca tgaaaatacc gaccacggca gcaaccacta agaacaaaaa gtatatcatc
    23341 gcatttaagg tcatattttg ggcagaatag ccaggaatac tttcaatgaa tgtcccaata
    23401 gttaataact gtagaccctt cgatgctttt tctttcgaaa ttttagctga atcctttatt
    23461 acgatcccat tgatcggctg ttcttcttca gaagcaaacg gctgttgtcc atacttcaaa
    23521 gcagtccacg tatctaggga cgcatagatg acgggagaaa ctgtgtaata agtttcagga
    23581 aagataccga caatcgtcaa aggatcgtcg taactgccaa ttttgatcgt atctccaatt
    23641 ttatagcctg aatcagccag attttgagag atgatgattt cattctttgt tttaaattca
    23701 tgccctttcg tgagttttgg taataaaaat gcttctttcg ttgtgccgaa aactgtaaca
    23761 ttttctttgt tttttccttt gattgctccg ctgtacaatc cgattggtgc tttctctcct
    23821 ccttcaatgt atttcagatc tcctcttgtt aattgtgaag ctgcaaatac ttgatttgca
    23881 tcctccgaca aaacaatctc ttgtgcgtcc caatcatcga ttgctttttt aaactcttct
    23941 gaaagtccat tcgctagtcc tgacaacatg aatacgacat acgcaatcaa gaacataata
    24001 ccgacgatca agccatatcg taatttagaa tagcgaattt ctttgatagc tagaaacatt
    24061 tgtttttcct ccctatgata gattgatagt gacaaaatgt cacttgttga atagtgtaac
    24121 tctaaagtga caatttgtca cttgtgttgt ttttttctta tcaataaatt gtctaagaca
    24181 tctttcgata aaaccttaac atgagtcata aaaaatttat ttaagcctta tttatgcttt
    24241 ccatgataaa ctaaagtgac caaatcaatg aggtgggaaa atgattaaac tattagtagt
    24301 agaagacgat aaagtattat ccaataatat aaaagaaata ctcgctgcaa tcggcgagat
    24361 aacacaagtt tatgatggta gtgaaggact gttcgagttg gaaagtggag tttatgatct
    24421 tgcaatcttg gatttgatga tgccggaaat gaatggatat gaagtgttag caaaaatacg
    24481 gaaagaaaaa atacagactc cagtattgat tttgactgca aaagatagtt tggaagataa
    24541 gattgaagga tttgaacgag gtgcagatga ttatctaaca aaacctttct atcgagaaga
    24601 actgatcatg cgcgtgaaag cattattgaa acgctcgcta ggtctctttg atgaaaatgt
    24661 tctgaagtat aaggatatca gttgtgattt aaaaagcaat gaagtcttgt ataaagaaca
    24721 agttttgccc atacaaggaa aagagtttga attgctcgtt tattttttac aaaataaggg
    24781 aatcattttg acaaaagagc agatatttga ccggatctgg ggatttgatt ctgatacgac
    24841 gattacagtt attgaagttt atatgagcaa cttacgaaaa catctgcgaa gtactggatt
    24901 ggataaagaa atcaaaacct tacgtaatgt cggttatata ctgcaaaggg tgtgatgcaa
    24961 tgaatggaga tattacacga aagcaacgta gtcgcttttt tgcgacaaat gtagcagcat
    25021 ttgcttttat cttttttctt ttgggattca ttactttaca gatattaaac caatctgcat
    25081 acagagaaac agatctgtca ttagagaaaa taaattttga ttctcgaaca gtacaattag
    25141 agatacaaag atatcagcaa ggcgatccct ttttaaaccc gactcaaacg gaacaaaaca
    25201 gaacaaacat ggaacagcca ttaatggaac catcaaatga ccgttttaac actcaagtga
    25261 tcttgtggtc taaaaatggt gagatattga ataaagcagc tattggtggg cgtttcaacc
    25321 aaatcaaaga attaaaaatg gatacgaaaa atctgaatac catccaaaat attgaattaa
    25381 atgaagacag tggctcatct gccctctctt tccgttcgat tacgaaaaaa gcggagacga
    25441 atcaagatgg cgttgcctac atccaaattt tagaaaatac aaatcaaatc aagaattcat
    25501 tagcgacttt tcgcacgatt atgattttgt gtatgattgt tttttggatt ctctctattg
    25561 gtatcagtta ttacttgtct agtctaagca tgcgccctat tattctttct tggagaagac
    25621 aaaaagaatt tgttgagaat gcttctcatg aattgcgcac accgttaacg attattcaaa
    25681 atagtttgga acatcttttt accaagccta atcatactat tattgaagaa tccgaaagca
    25741 tcgctcaagc attgtcagag acgaggcgcc tcactggatt gacttcggat ttgctgacaa
    25801 tcgctcggaa tgattctgat cagcagctgc tttcaaagca aatgatcaat acacaagaat
    25861 atatcgaaca acttgttaaa ccatttcaag agatggccat catagatgga aaagagttta
    25921 ttctccataa cgatgcaaca ttagaagtac caattgatga aaaaaaaatc catcaagttt
    25981 tggttatttt gctggataat gcattaaaat atacgaatcc agaagataag atcattttgg
    26041 attcatctgt taaccataaa gaatggctga ttcacgtaaa gaatactggc ccttctatca
    26101 gtgaagaaga tcaagcaagg atttttgatc gattttatcg ggaagatcaa tctcgggcga
    26161 aagagactgg cggatacggg cttggcttgg caattgcaaa acaggtaatc gaacagcatc
    26221 atggacagat ttctgtatcc aattatcttc cacaaggagt cgattttaaa gtccaattgc
    26281 cgttgaagcc taaaaaataa tgcttatccc taaaaaaggg ctgtgacaaa aatcttgtca
    26341 cagccctttt ttgttgcgaa taaacggtgt tccagaagta gcttcttcgg aaataagccg
    26401 aaatttcttt aagctgacca cttctgtcac aacctcttta gcttacgctt acgatttttt
    26461 attgcggtct tcaatgtgta catcaccgct tgtgtcaata tccaattctt ctcggctaac
    26521 atcttctgtc actttttcaa catcttcatg ttcttcttta tgaatcccaa cttcttcacg
    26581 aataactgta tgtttgctta catctacttg ctcttctttg attgggatag taatcgtgtc
    26641 atcttccaat ttttcgtctg ttccctgtga agattgatct gttactggtt tgcgttcgat
    26701 gacgatttct tcacgtttga ccggtacttc taccgtttca gtatcgttga ccacatgttt
    26761 gtggatgtcg acttctccag ttgtcacatc atgtgtattt acatctaatt tttcttcttt
    26821 tagttgaatt gtatcatcat ctagatttgg tttgcgatcg atgtcttcag tcttgcttgt
    26881 acccattgta tcagtctcgc caggtacgtc catcggatca gttcctggtg tcatattcaa
    26941 tggatccgtt gtattttgaa agcctgtcgt gccacctacg tccattggat ctgttccagt
    27001 gccagtttct aatccagtcc ccattgtgct tgctgggcta acagtatctt gtgtatccat
    27061 tgtatcttct gttgtttctg gctgacgata gccttttact gtgatgacaa attttccatc
    27121 agaaatatct cctctatatt gatataaagg atcattttct ggtgtagccg tctcagaatc
    27181 ataggtatca aaagaaaaag tatcttttat tttttcccat aaagaacggt cttcttcacc
    27241 atccatccga ttttcatctg tttcaacatc tataccagac aatccgtcaa atcctagtct
    27301 agatgctgct tcatcagtag tataaataac gatatcatct cgttcatacc cttcgttacg
    27361 caaccgttca attactgata atgcttcttc cctcgtacta tagcttccga tgaccgttaa
    27421 atttttctct tccatgtaaa ttcctcctag caaatagatt tatgattggt atattgttac
    27481 ttataaattt attctatagg agacgattgt
//
LOCUS       DAYYKN010000025        26483 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000025 DAYYKN010000000
VERSION     DAYYKN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.025"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..262)
                     /locus_tag="ACHBB7_000904"
     CDS             complement(<1..262)
                     /locus_tag="ACHBB7_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9208872.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKL"
     gene            complement(376..468)
                     /locus_tag="ACHBB7_000905"
                     /pseudo
     CDS             complement(376..468)
                     /locus_tag="ACHBB7_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(604..1239)
                     /locus_tag="ACHBB7_000906"
     CDS             complement(604..1239)
                     /locus_tag="ACHBB7_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9208873.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1751..2893
                     /locus_tag="ACHBB7_000907"
     CDS             1751..2893
                     /locus_tag="ACHBB7_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9208874.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            2886..3092
                     /locus_tag="ACHBB7_000908"
     CDS             2886..3092
                     /locus_tag="ACHBB7_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM9208875.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            3171..4364
                     /locus_tag="ACHBB7_000909"
     CDS             3171..4364
                     /locus_tag="ACHBB7_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9208876.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            4569..5708
                     /locus_tag="ACHBB7_000910"
     CDS             4569..5708
                     /locus_tag="ACHBB7_000910"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM9208877.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5701..6501
                     /locus_tag="ACHBB7_000911"
     CDS             5701..6501
                     /locus_tag="ACHBB7_000911"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9208878.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6501..7328
                     /locus_tag="ACHBB7_000912"
     CDS             6501..7328
                     /locus_tag="ACHBB7_000912"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9208879.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7333..8040
                     /locus_tag="ACHBB7_000913"
     CDS             7333..8040
                     /locus_tag="ACHBB7_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9208880.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8140..9006
                     /gene="rfbA"
                     /locus_tag="ACHBB7_000914"
     CDS             8140..9006
                     /gene="rfbA"
                     /locus_tag="ACHBB7_000914"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM9208881.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9020..9592
                     /gene="rfbC"
                     /locus_tag="ACHBB7_000915"
     CDS             9020..9592
                     /gene="rfbC"
                     /locus_tag="ACHBB7_000915"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM9208882.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9614..10642
                     /gene="rfbB"
                     /locus_tag="ACHBB7_000916"
     CDS             9614..10642
                     /gene="rfbB"
                     /locus_tag="ACHBB7_000916"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM9208883.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10740..11591
                     /gene="rfbD"
                     /locus_tag="ACHBB7_000917"
     CDS             10740..11591
                     /gene="rfbD"
                     /locus_tag="ACHBB7_000917"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM9208884.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11625..13658
                     /locus_tag="ACHBB7_000918"
     CDS             11625..13658
                     /locus_tag="ACHBB7_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208885.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13702..14982
                     /locus_tag="ACHBB7_000919"
     CDS             13702..14982
                     /locus_tag="ACHBB7_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM9208886.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15192..15998
                     /locus_tag="ACHBB7_000920"
     CDS             15192..15998
                     /locus_tag="ACHBB7_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9208887.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16010..17230
                     /locus_tag="ACHBB7_000921"
     CDS             16010..17230
                     /locus_tag="ACHBB7_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9208888.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17220..18806
                     /locus_tag="ACHBB7_000922"
     CDS             17220..18806
                     /locus_tag="ACHBB7_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9208889.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            18845..20983
                     /locus_tag="ACHBB7_000923"
     CDS             18845..20983
                     /locus_tag="ACHBB7_000923"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9208890.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21351..22334)
                     /locus_tag="ACHBB7_000924"
     CDS             complement(21351..22334)
                     /locus_tag="ACHBB7_000924"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9208891.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22712..24103
                     /locus_tag="ACHBB7_000925"
     CDS             22712..24103
                     /locus_tag="ACHBB7_000925"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9208892.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24119..25159
                     /locus_tag="ACHBB7_000926"
     CDS             24119..25159
                     /locus_tag="ACHBB7_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9208893.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25178..26263
                     /locus_tag="ACHBB7_000927"
     CDS             25178..26263
                     /locus_tag="ACHBB7_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM9208894.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26296..>26483
                     /locus_tag="ACHBB7_000928"
     CDS             26296..>26483
                     /locus_tag="ACHBB7_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFM9208895.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVK"
BASE COUNT         8597 a         4444 c         5382 g         8060 t
ORIGIN      
        1 ttaacttttc taaaccgttt aaactatcgt tatctctaat tttagcaaat attactgcat
       61 tttcatttcc aattctttga aaaattgctt ttaaaatatc ttcctttgaa tcgaaatgat
      121 gataaatagc acctttagaa agttttgttt cattgataat atcttgcaat gatgtgttat
      181 catatccttt ttcacaaaac atttttgtag aaacatctaa aatgagtttt actgtttctt
      241 ctggatattt atttcgtgcc ataacttacc tccaatacaa accgttggtc tgattaaagt
      301 atatcttagt attttatgct tgtcaatata aagacttgca attttataaa aatgcagaat
      361 gcaagccttt ttcatttatt ctacaatctt ccagttgctt tccttatgcg gtgtcaattc
      421 atactgtgat acctgcgttg cctttgcctg atttaccaca aatcagattt tatccctttc
      481 ttcctcttga ttgcccacaa cagaaaagaa aaagccctgt caagagcttt gccaccattg
      541 gtggtgcttt gcactcttga tcgggctttt tgtttgctgt atcttctcgc aagaggaaaa
      601 aagttactcg tcctcatcat caaaattgtt attccttaat acttctcgta acagttccat
      661 atcttctttt gagttcgggt ttatcatttt cacgacttga taaggtgtct tatacttatg
      721 tccctctatg ctttccccaa acatatccat actgtataaa tcatcatagc ggtctaataa
      781 tttttgaaac ttcaaatgct ggataaattc tttgattggg taaaggtcgg acgcttcaat
      841 gcttttcccg ttgatatagt ctgtaatata ttcccgtaac ttttctaact catattccag
      901 ccattcttcc tcgctgtcaa agaagttatc atagtgtcca tgtttcagtt ctgcatttag
      961 acgttcttcg gctcttaaaa actgacagac ttccttttca gattgattga catacttcca
     1021 ttctcccgac aacaattcat tgggcgtaac gtccagcact tccattatct tttccagcgt
     1081 ttcataggca ggatagttca accctctttc aatcttggaa aggctctgca tattgatacc
     1141 gattttgtcc gcaagttcct gttgtttcat tcccctaaat tttcttatgg tctgtatatt
     1201 atttcctaaa tggcttattt tcattgcata accctccata tctcgttata tcttcgctat
     1261 tataagtcta tcatgcttaa tatgatttgt aaaggcaaaa aaagatattg acaaataagc
     1321 atataagaat tactatgttc ttgtaatcga ataagcacaa tagaatgaaa tataaaaaca
     1381 ttcaaaaaga aatacctatc tgattaaatc cgtagatatg aatatcggtt gaaatggaac
     1441 ttgttagaaa aggcaaaaca gaacttgctg acaaagtatt tctgacacgg tggcggaagc
     1501 ctctcaggct taccgccatg acaccttgtg tcaaggggaa gaaatgcttt gtcggctggc
     1561 gagcctgcga gccttgtctg tgcccccgtt atctaacagg ggggcactat ttcacaaaag
     1621 acaagtcaaa aaccatacac ccccaaacct gtgagtagta atgagaggtt ttaagaagat
     1681 agtacatatc acactttttg aaaggtggtt tacaggtatt tcaaggctat tgtgggattg
     1741 gaggaataca atgaacaatt tagaatttgc acttgaaatg aagaacaaac gtcttgccag
     1801 cggtctttca caaggagaac ttgcaagtct tacccatgta tcaagatata gtatcaaccg
     1861 ctttgagaac ggaaaagcta acgccagcaa ggaaacacag aacattatcc tgcgttgtct
     1921 gaattactat atctgcgata agccttttta tctgctcgtt gattatctgt ctgtccgttt
     1981 tccaacgact gacgcattgg aagttatccg aaaggtgctt ggcatgaaag cagattattt
     2041 tatccattat gactatgggt attatggcta taaggagcat tatgcttacg gggaaatcaa
     2101 ggtcatggct tctgatgatg aacacatggg cgtattttta gaattaaaag gggcaggctc
     2161 acgcaacatg gaatatgtct tacaggcaca gaacagggac tggtattcat tcttaaaccg
     2221 ctgtcttgac tgtggcggtg ttatccgacg ttttgacttg gcaatcaatg atatgtgcgg
     2281 tctgctggat attcctgtgc tatctgaaaa atacagaaat ggtggtgcgg aatgtcgctg
     2341 taaaaactat gaaaatgtac aaggtggaaa attaagcgga aaaaaccgta atttagcaag
     2401 taccctttat atcggctcaa aggcaagcac aaaatatttc tgcctgtatg aaaaacaaaa
     2461 ggaacaggca acgaaaaaga aacataccga tattatcaac cgttttgaaa ttcgtctgcg
     2521 tgacaaaaag gcggtacagg cagttgaaga actgttattg acgtataacc ctcatgggct
     2581 ggtgttttat ctcattaccg attttgtgga atttcctgac tatccattgt gggagatatt
     2641 catttcccat gacagtttac cttttgaaat gaaccctgta cctgtcaata tggaacgcac
     2701 tctgcaatgg ctggaaagac aggtcatgcc gtcaatcgtg atgatagagg aaatcgacag
     2761 gctgacaggt tcaaactaca tgaaaatgat tgatgaatgt acccaccttt ctgaaaaaca
     2821 ggaaatgctt gtggaacaga tgtgtacgga catatcagac gtaatcgaaa gtgagggggt
     2881 gttttatgag taatacacag gatattcccg tatgggaaaa atacaccctt accattgaag
     2941 aagcgtcaaa gtatttccgc attggagaaa acaagttaag acgattggca gaggaaaaca
     3001 aggacgctgg ctggctcatt atgaatggca accgcataca gattaaacgc cgacagtttg
     3061 aaaaggttat tgataaattg gacgcaatct aatgcaaatg agccttgtat gtgttatgat
     3121 gaacacaagt catatcaagg ctctttccaa caaggaaagg agcagacacc atgaaagaaa
     3181 aaagacggga taacaaaggt cgtatcctgc atactggaga gagccaacga acagacggaa
     3241 aatacttata taaatatgta gacgcattgg gaaacacaaa atatgtgtat gcttggagat
     3301 tgacacccac agacccgaca ccaaagggaa aacgggaaaa accctcactt cgagaactgg
     3361 aacagcagat aagacgggat attgaggacg gtatcgacag cacaggcaag aaaatgacgc
     3421 tttgccaact ctatgccaaa cagaacgcac agaggacaaa tgtgaagaaa agcacacaga
     3481 aacaacggga acaactcatg cggttattaa aagaggacaa gttaggtgct aggagcattg
     3541 atacgataaa accctctgac gctaaagaat gggcgttacg catgaaagac aaaggctttt
     3601 cctacaatac cattaacaac cataaacgct cgttaaaagc gtcattctac atagccatac
     3661 aagatgatta tgtgaggaaa aacccttttg atttcaagct aagtgaagtc atagaaaatg
     3721 ataccaaaga gaaagtcgca ttgacagagg aacaggagca agccttattg tcatttatta
     3781 agacggacaa cgtgtatcac aagcattatg atgatgtgct gatactgtta aagacaggac
     3841 ttcgtatctc ggaactgtgc ggactgacag tagccgacat tgatttcaag aatgaagttg
     3901 tgattatcga ccaccagtta ctaaagagta aggaacaggg ctattatatt gaaacgccta
     3961 agacgaaaag cgggataagg caagtgccat taagcaaaga aacgatacag gcatttcaac
     4021 gggttatcaa cagccgacca aagataaagt cgatagaggt agacggacac agcggttttg
     4081 tgtttatcaa tccgaaaggt aatccaaaag ttgccattga ttataacgcc ttatttatcc
     4141 gtatggtaaa gaaatacaac aagcaccaca aggacaatcc cttgccacat attacaccgc
     4201 atacgctacg ccatacattc tgcacaaggc tggcaagcaa gaacatgaac ccgaaagatt
     4261 tacagtatat catggggcat tcaaacatca gtatcacaat gaactggtac gctcatgcgt
     4321 ccatagatac cgcaaaatca gaggttcagc gtctaatcgc atagaagtat ttaccacgat
     4381 tttaaccacg attgacaggg aaaatataag aagatagacc tagatatgcg aggtttacca
     4441 caaaagcaaa atgcccgtag agccgataaa ataaggcttt gcggacattt gagaagatat
     4501 aaaaagatag tcaaaaagac atatataatt tataataatt atgactctta attaaagtag
     4561 gtgcccatat gcttgtttct tttagtatgg ttataagatt ttttctgaca attttattat
     4621 cactgatatt aacacctgta ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac
     4681 caaatgaaag aagaataaat aaagttccaa tgccttcagc gggcggactg cctatcttta
     4741 tttcatttgt tatatcgact ttattcttat tccgtaaaat tatccctcaa tcgtatattg
     4801 taccgatctt gattgcttct ttagtaatca tagtcacagg agtgctcgat gataaatatg
     4861 agctgacacc taaacaaaaa agtataggga tcttgattgc ttctcttatt atttattttg
     4921 tagcagatat acgtattgat tccttcacgc ttccttttat tggatatatc caactaggct
     4981 ggcttagttt tcccgtaacg attttttgga tcttcggtat cacgaatgcg gtcaatctga
     5041 ttgacggttt ggatgggctg gcggcaggga tttcattgat cggtttaatt acgataggaa
     5101 tcattggata ttttttcctc catgcttcta cagtgtatat tccagttgtt attttctgtc
     5161 ttgtagcaag tattatcggt ttttttcctt acaattttta tccggcaaag atttatttag
     5221 gcgatacagg agctctattc ctagggttta tgatggccgt cctttcacta caagggctga
     5281 agaacgtgac ttttgtttca tcgatttctt tgctgatcgt catgggagtg ccggtaacag
     5341 acacgttttt tgctatcatt aggagaaaag caaaccgtgt ttctttttct acagcagata
     5401 aaaaacatct tcatcaccga ctaatcagtt taggctttac tcataaaggt gctgtcctga
     5461 cgatttatgc gatggctttg atgttttctt tcacagctat ggtcatgaat tatactggaa
     5521 gaatcggaac gatcatattg attattgcca tgttgtttgc tgcaatttta ttatttgaat
     5581 tgattggtct gatcaatgag aaacatcagt ttattttaaa aacattgcga tttttaggga
     5641 ataaagagta ccgtacgcaa gtaaaacaga aatatttgaa gaaatggaac cgtcatatag
     5701 atgaataaca aattaaacca gccgatcctt attagtattg tgacggcaaa cagtgataaa
     5761 atttttcgga cactagataa ttttatttca actgtaaaag atgaaacagc agtaaaaatc
     5821 cgaatttttg acaacaattc acttcccgag tacaaaaaaa gactaacaga gtataggaaa
     5881 tatccctaca tcgatatcta ttttcatgat gaaaattccg gatttggtta tggacataat
     5941 cacaatctga tagctagcga ttctcgctat ggcattattt gtaacccaga tattttagtg
     6001 gatgaagaga cagttacagc attggtcttg ttattgaaag aacatccgga atgtgcgatg
     6061 ttagcaccta agattcttaa tgaagatgga acaactcagc acctcatccg tgagcagctg
     6121 gctgtttttg attacgtttt gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac
     6181 aaacgactgg ctgtgtttga atgccgagga ttgcctactg atgctaatag ttatgtacgg
     6241 atgatctcag gtagttttat ggtagctgat attgataaat ttaaagagat aaaggggttt
     6301 gatgagcgtt tcttcatgta ctttgaagac aacgacttgt gtttgagttt tgaaaaagca
     6361 ggttacaagt tattgtacac acccttttat gcagttatcc atttttacgg gaagggtgcg
     6421 catcgaaatg caaaactgtt tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag
     6481 tgggggtggc gttttttctg atgaaaccag aaatattagt agtcgttgtg ctttatcagc
     6541 aactattcag tcaatctcca gcttatggac cattaagcaa agcacttagc gaaaagcaaa
     6601 ttcagcttat tatttacgat aatagtcctc aaaggcagca aaatgagctg tttgaaaaag
     6661 aaaatatcgt ttatcatcat gatccgcaaa atcctggttt agcggtggct tataattttg
     6721 cgttgtctca agcagatgaa ggaacacgtt gtctcgtcac tctggatcag gatacgaggc
     6781 tagtcgacga ttattttgaa ctattgcgta aagtaacctt tacagatgaa tgtgttgctg
     6841 cagttccgat gattttttct gggagccgac aagtttctcc cctatattca gatcgttata
     6901 tcaatcgtac agctcaagca gtagaagttg aaggcccaac agctgaacga atcatggcaa
     6961 tcaattcagg tgttgcctgg tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg
     7021 ctttttcttt agattttttg gatcattggc tgttttggaa agtggatcag ttggaaaaaa
     7081 ctgtggagat ccttcctgta cgaatggaac atgatctttc ggttttagac tatgaaaaag
     7141 tttcaagtaa tcgataccgt tctattttac aagcagaaaa tcgattttat catgaatatg
     7201 atcaaaggca tttatccagt caccgacgac aattattgtt aagaacagct aaacagttct
     7261 tgaccgtcaa aaaccggcat atctggcgga tgactttgcg ctcatatgtt aagaattgga
     7321 aggtgtgaat tcgtgcgttc ggtttgtata gctaccttta atggaggcag atatttaaga
     7381 gagcagttag atagtatttt atctcagctt tcagcagaag atgaagtagt catttctgat
     7441 gatgggtcag tagatgatac atgggatata ttaactgaat acgcagaaaa agataagcga
     7501 atccagcttt tttcggggcc tcgtcaagga ttgatcgcta attttggtta tgcgatccag
     7561 cagacgaaag gagatctaat ctttttggca gaccaagatg atgtttggtt ggagacaaaa
     7621 gtgaaaaaga tcactgacta ttttgagcag aatccggatc agctagtagt agtttctgat
     7681 ttaatcattg tggacagcga gctgcaagag atccatgatt cgtatttttc gtttcgaaat
     7741 tccaaagaag gatggcttaa taatgtgata agaagtcact atattggtgc tggtatggct
     7801 ttccggtctt ctttaaaacc cgatattttg ccgatcccgt cagaagtgcc gatgcatgat
     7861 atgtggattg gtttgctggg aggaaagaag actgggttcc taagagaacc gttaactctg
     7921 taccgaagac atgatttaaa cgcgagtgag attgaaacac attctaaatt acggcaaaaa
     7981 atttcttggc gtgttcatct gattacggcg ttgatcaaac gaaaatggtt taaacgttga
     8041 taaaagatgt ttaaatcctt aatatttcca tgacaaatat aacaattgat gagataataa
     8101 caaagtgaaa tttgaaatca aaaaggagag cagatatcca tgaagggaat cattcttgct
     8161 gggggcagcg gaacacgtct gtacccttta acaaaggcaa catcgaaaca attgatgccg
     8221 atttatgaca aaccaatgat ttattatcca atgtctactc tgatgttggc gggaatcaaa
     8281 gaaattctga ttatttcaac gccacaagat acaccacgtt tcaaggaatt atttggaaac
     8341 ggaaatgatt taggactgca gatcgaatat gcagtacaag aaagtccaga tggattggca
     8401 caagcgttta tcattggaga agaatttatt ggagatgata gtgtctgctt ggtattaggg
     8461 gacaatattt actatggtgg cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat
     8521 acaggcgcga cagtatttgg ctatcatgtc aatgatccag aacgctttgg tgtagtagaa
     8581 tttgatgaga atatgcaagc tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat
     8641 tacgctgtaa ctggccttta tttttatgac aatgaggtaa tttctatcgc taaaggtatc
     8701 aaaccttcag aacgtggaga actagagatt acggatgtca ataaagcata tttggataaa
     8761 ggaaaactat ccgtagaact tatgggacgc ggatttgctt ggcttgatac aggaacacat
     8821 gaatcattgc ttgaagcatc tacctttatc gaaacaattg aaaaacgtca gaacttaaaa
     8881 gtggcttgtt tggaagaaat tgcttaccgt atgggctaca tcgataagga acaattgctg
     8941 gcccttgcac agccattgaa aaaaaatcaa tacggtcaat atttattgaa tttggccgct
     9001 gaataaggag tccttacaga tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt
     9061 agaaatggac gtctttggcg atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa
     9121 atttgcagaa catggattga aatatgactt tgttcaagac aaccattcac tatcagctga
     9181 agctggtgtc cttcgggggc ttcatttcca aaaaggagaa gctgcacaga caaaattgat
     9241 tcgggtagtc actggagcag ttttagatgt agtagtggat atccgcaaag gtagcccgac
     9301 ttatggtcaa tgggacggat ttatcttatc tgaacataat catcgacagt tgcttgtacc
     9361 taaaggatat gcccatggat tcgttacttt gacaccaaat gtgaacttcc tttataagtg
     9421 tgataattac tataatgcag aagcagatgg gggcatcgca tttgatgatc cagatttagc
     9481 gattgattgg ccaattgatc catcaagagc gattatgtcg gaaaaagatc accgtcatcc
     9541 gacattaaaa gaatttgaag cagaaaatcc atttgtttac ggagaaatct aagaattaag
     9601 gagaaatttt cttatgaaaa acatcattgt taccggaggt gccggattca tcggctctaa
     9661 tttcgttcat tacgtggtaa acaatcatcc agaagtgcat gtcactgttt tggataaact
     9721 gacttatgca ggaaacaaag agaacttggc tggacttcct agcgatcgcg tagaattggt
     9781 cgtaggagat gttgcagatg cagaattagt ggaccgtcta gtaaaagaag cggacgcagt
     9841 tgttcattac gcggcagaat cccataacga taattcatta aaagatcctt tcccatttgt
     9901 acaaacgaat atcattggta cgtatacact tttagaagct tgccgcaagt atgatgtacg
     9961 ctatcatcac gtatctactg acgaggttta cggcgatctt ccattacgtg aagacctgcc
    10021 aggacatggg gaaggcgaag gagaaaaatt cactgcggaa acaccatata atccttcaag
    10081 tccatattct tctacaaaag ctggttcaga tctgttagta aaagcatggg tgcgttcttt
    10141 tggcttacgt gcaaccattt caaactgttc aaacaactat ggtccttatc agcatattga
    10201 aaaatttatt cctcgccaag taacgaatat cttaagcggt attcgtccaa aattatatgg
    10261 cgaaggaaag aatgttcgtg actggatcca tacgaatgat cattcttcag cagtgtggct
    10321 gattttgacg aaaggcagaa ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga
    10381 taataagacc gtgatggaat tgatcttaga aatgatgggt caaccaaaag atgcgtacga
    10441 ccatgtaaac gaccgtgcag gacacgactt gcgttatgca atcgattcga caaaattacg
    10501 ggaagagtta ggatggaagc ctgagtttac gaacttccgt gatgggttgg ctgacacgat
    10561 caaatggtac gaagaacatg aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc
    10621 atatgccaaa aatggacaat aatacagaga aaatgtcact gtattaatgg aaaaacacat
    10681 gtgaggcagg cgctcgcctg tctctttttt aaaataaaaa tcaaggagcg gataatcata
    10741 tgtttttatt aactggagga aatgggcaac taggtacaga attgcgccat ttattagatg
    10801 aaaaaaatct gcaatatgtt tcaacagatg caaaagactt ggatatcaca gatgccgaga
    10861 aaacaatggc ttacatcaca gaattaaaac ctgatgttat tttccattgt gcagcttaca
    10921 cagcagtaga taaagcagaa gaagaagcaa aagaattaga tgagaagatc aatgttgatg
    10981 gtacaagaaa tgtagcactt gcagcaaaag ctgtgaatgc tcaattcgtc tatatcagta
    11041 cagactatgt tttcgatgga aaaaacaaaa atgtagaata cgaagtagac gaccaaacga
    11101 atccactaaa tgaatatggt cgtacgaaat tattaggcga aaaagcagta gaagagattt
    11161 tagaagacta ttatatcata cgaacttctt gggttttcgg gatctatgga cacaatttcg
    11221 tcttcacgat gcaacgccta gcggaaaccc gcgacaaact gactgtagta aatgaccagt
    11281 ttgggcgacc aacttggaca cgtactttgg cagaatttat ggtatatgtc atcgaagaaa
    11341 aagcaccatt tggtatttat catttgtcga acgaaaatag ctgttcttgg tatgaattcg
    11401 caaaagaaat attgaaagat acagatgtag aagttgcacc tgttacgtca gaagaatttc
    11461 cgcaaaaagc aacacgtccg cagtattcag tcatgagcct gaaaaagaca gaagagttag
    11521 gatttgtcat accaacttgg caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa
    11581 tgaataagta atttcctttt atcaaataga atttggagca tgatatggaa aaacaaacaa
    11641 gaagtgcacg acgaaaagaa aagccaaaag cgaaccaggc agattcgaat ttggtgcttg
    11701 acctgtttct aaaactccgc tatcttattg gcgttttagt catcgtggtc atcgttactt
    11761 ttaatttaaa cggaagttca cttggtgttt gggataaata tgtgtcgcaa agagacgatg
    11821 ggaaaaaaac tgatgtgatt tttggacaga atcgggaagt gcggtcagac gaatggctgg
    11881 tgcagacacc tttttacctc tcacaagctg aagaggactt tccgttggtg aacgaggatt
    11941 attcattaaa cggacaaaac atgatcattg cgtataattc tcctgtaaag gatattacgg
    12001 ttatcggaaa accattcaac tggggattct ttttcctcgg aagagatcga ggactttcct
    12061 tttattgggc gatgaaatta gtcgtcatgg tcttgcttgg attcgaagta ctgatgatat
    12121 taacgaagag aaaaaaagct ctatcgctaa tcggtgcatt tgcgctgaca ttttctccag
    12181 cagtccagtg gtggttcatg caacatgtgg gcgatttgat tttctttact ttgggactga
    12241 tgactgcttt ttatcattat ttctacaatc atgataaaaa atggctacga acgttgatga
    12301 tgcttttggt ggttattttc ggtattgggt ttattttagt gatttaccca gcccatcaag
    12361 tgatgctggc gtacttgctg attttttatt ttgttggcct gcttatttac tatagcaaga
    12421 aagtttcatg ggatctgttg gatgtagtgt tgattggagg ggcagtttta tttattggcg
    12481 gagtcatggt tcatttctgg ctaacgtcaa aagacgcatt gttagcttct ttgaatacct
    12541 tatatccagg aaaacgggta agtactggag gaaagtggac gattggcgaa tttttttctt
    12601 tccttacaaa ttggaaaatt ccttttgaag atatcaacta ttcgaataac tctgaagtgg
    12661 ccctgttcta tcactttttt ccaactgtat tcctagcaag tccatttgtg ttatttggca
    12721 aaaaagatag cgaacaaaag ttactgggac gtattttgat gattttttgt ctgttttcta
    12781 tcttttggat taccgttggg ttgccaaaat ggctagcaga aatcactctg ctgtcttatg
    12841 ttcctccatt aagagccatc cttactttta gttttgcggc ttgtttatta tcgatctggt
    12901 ttatcgctta tatttggaga gaagatgtta ttcctatttg gtataaattc attttgttgg
    12961 cagtaaatgc atggtcttat ttctatgcga ttacgcattc gaaaatggat aattattttt
    13021 cggattttga aaccatcgca gttgctgtgt tgggaacagt gattttagct tttgcattat
    13081 ttggctggag acgcttgttt tacctcgctt ttgccgcctt agttatcgta tctggctttt
    13141 tcgtcaatcc agtcgtagaa ggtacaggag cgatttttga aaaaacactg gcaaaagaaa
    13201 tccaaaaaat cgatcaacaa gatcctggta aagtttggtt gtctgaagat gaattatata
    13261 acttcacccc aacattggga gtgaaatcct tcaatacagt acgcttttat ccagatatag
    13321 ctgcatggaa gaaaatcgat ccgaaagaag aatacgagaa gtattataat cggtatgctc
    13381 atacaagagc ttttatcgct aaggaagata caaagttcat tttagatcgt ccagatatgt
    13441 tttcggtcac gttgaatttt gaagacgcag aaaaattagg gatcgactat gtgatcagta
    13501 aacgaccatt aactgattac aaccaattat atgaagcgca gttcacgcag ctatatggac
    13561 cggataaaga cggttatatg atttttaaag tagaatatcc ggattatcca gatgtaccac
    13621 aaacacaaac tcaggaaaat cagctagtcg tacagtaata ggctagctga gaataatcct
    13681 gaaatgttag tagaaaggag agtggagata attcaagcaa tcgtacaaaa atttgtcaac
    13741 aaaatgagtg tcggtttctt gcttttgtta ctgacgctag tcttttttgt ttggacagca
    13801 gggttaggtt ttccagcaac tttgtttata tataacaata gttcattact tatcgctttt
    13861 agtgtggcta ttttgctcat tttaaatgtt tataaagcaa aaggcgtcga ttttctttat
    13921 ctaggtgttg cgcttgccct tttcattttc ttttcttact ttcgggaatt aagaaatgag
    13981 tctttatttg gagacttact gtttcctttg attattttat tttgtttagt aattaaatgg
    14041 atcgagtgga caaaatggga tcgcggtatt tatctgattg tcttttcggt cttcctcgga
    14101 atcacgttat atcgtgtatt tacagagatc caagtaccag aaggacaaag catctgggct
    14161 ccaggcaatt cgctttctga tatttggatc aatacgaata taatcggaag ttctttgatg
    14221 actcttgctt tattgatcag tggatttgcc agttcttttg aacgatggta tgtacgaatc
    14281 atcggtgcag tagcagttgt tgcagctttt ctcgggattt gggtctgcca atctagaggt
    14341 gcattagtcg cgttgattgt ttttgcgata ttggatcttt tacctaaatc gttgatgcgt
    14401 gtcattcgtg cgccatttat tgcttacacg gttacgattc ttttagcttt gccgatttct
    14461 tatctggcgg ctgtctctga aaaagtcaat ttgtttaccg gtcgagaaga tatttggcat
    14521 aaattctatc agacgctagg agaaaaatct gaacaaattt tactaggaat gaaaacattt
    14581 atcttccaac gaggaaacca atttttagga aaccacaata gttataattc tattttgaat
    14641 atctatggat tgataggatt tgggattgct gctttgctgc ttatcctttt tattggtcgc
    14701 ttaacgttga aagcagattt atccaacggg cagatgactt ttatctgggc cttttttgcg
    14761 gtgatgatgc agtcatttat ggaggataca ttgacttcat acccatgggt tccgattatt
    14821 tatatcctgt tggctatggc cgttcatcga tatgatgaac cagaagttgc ggaggaattc
    14881 tatgttatgg aagaggcata tgacgatatg acttatgatg atacatctta tgaggaacaa
    14941 ccagatagcg ctcattcttc caggatccaa cgctatcatt gataggcaca aaatacgaga
    15001 gaggctgtga caaccgtcgc agcctttttt ggtttattgc aattgtctag catcttctgt
    15061 aaaatagaaa cgttagagaa aatcttgtat catagaaaat taagaaagaa ataagaacgc
    15121 agctcggtac caaaaatatg gtaaaatggc acttgactgt aattcactga aaatcgcttg
    15181 gaggaacaca aatgtttaaa gatttgtatt tatttatgaa ggacatttat cagaacagaa
    15241 aattattgct acagttttcg atcaatgact tcaaatcaag atatgcagga tcgtttttag
    15301 gaatcttatg ggcgtttatc aatccgctat ttactgtatt gatttattgg ctggtatttg
    15361 gtgttggact gaaagcacaa atgatcgatg caaaataccc atttatcgta tatttagtta
    15421 ccgggatgat tccctggttc ttcttctcag atgcatttgc atcaactacg gttgtttttc
    15481 gggaatatac ttatttagta aagaaagtcg tattcaatat tcgtatcttg cccacgacga
    15541 aaatcctatc caatctgtac acccacttat tcttcattct gattggcata gctgtcgcgc
    15601 ttataaatgg tatatatcct tcagcaatgt ctttacagct gatttattat ttattttgca
    15661 tgtgtgcttt cttgacagga ttgacttggc tgacggcatc gatccaacca tttttacctg
    15721 atatcatgca attaattacg atcgtgctgc agttgatcat gtggacatta ccgatattat
    15781 ggagccctaa tcagtttgat tcaggtcttg taagcatatt gaaattaaat ccgctgtact
    15841 atgttgttca aggttatcgt gagtcatttt taagtgaagc ttggttctgg gagcatggaa
    15901 catatacttt gtatttctgg gttttcacgt tagtaatgtt cgtaatcggc tctgcagtgt
    15961 tccgtagatt gaaaccacat ttttcagatg tgctgtaagg agaatagcta tgacagaata
    16021 tgcaattaga ctaaaagata tcacaaaatc ttataatatg tacgcaaaac cttcggatcg
    16081 atttcgtgaa gccctcaatc cttttaagaa atcctatcat gatgtatttt atgcgttgaa
    16141 aaacgtgaat gtagaaataa aaaaaggtga gatgatcggt ttcgtgggag aaaacggatc
    16201 gggaaaatct acaatgttga aaatcattac gggagtactg acaccaacat ccggcacgat
    16261 ggaaatcgaa ggaaaaatct cagctttgct agaactagga tcaggattca atcctgagta
    16321 ttctggctat gaaaatatct atttaaatgg aatggttcta ggattttcaa gagaagaagt
    16381 cgatgaaatg gtcgatgata tcattgaatt tgctgacatc ggagaccata tttatcaacc
    16441 tgtaaaaact tattcaagcg ggatgttcgt tcgtctagct tttgctgtag caatcaatgt
    16501 gaatcccgat attttgattg tggatgaagc attggcagta ggcgatcttg aatttcaact
    16561 aaaatgtatg gaaaaattta cagaaatcaa gaactcggga aagacgattt tattcgtttc
    16621 tcatgatatc agttcgattc gtcgtttttg tgatcgttcc tattggttga aaaacggaga
    16681 agtcgtcgaa tatggcgata cgatggaagt aacgacgaat tatgaaaact ttttaaagaa
    16741 aaaatcagtt aaaacagtgg atcggaataa aaaccaagta gaacggttcg ccgctccaga
    16801 tatcgtcgac gtgacgaaag cggaattgtt ggataaagac aaaaatccgg tggatatgct
    16861 ggaacaagga gaaaaactat acgttaaagt aacttatgaa gtaaaagatg atacaatcaa
    16921 aaatccggtt ttaggaattg cgctacgaac agtcgacaat ttttatgtat gcggacttaa
    16981 tactctacta gatggtatca agattccttg gaaaaaagga gagaatatct tttatctgga
    17041 gtatgaaaag ttaggtttgt tagctggtga atattatttt gacgttgcgg tctttgaaga
    17101 aaatgcaacg gttcctttgg tatataagac aaagtacatg aacttgtttg tcagcggttc
    17161 ttatatcgga gaaggcatcg tggtcttaga ccataaatgg gaagaaggga ctcatagcaa
    17221 tgaaatatga ttttgaaatg gacctagatg aacaaagcag tgtgggaaaa attgccgcac
    17281 agatcaaacc aggaagtaaa gttttagagt ttggtcccgg aaacggccga ttaacgaaac
    17341 atttaatcgg agcaaaacaa tgtgaagtga gcattgttga gttagataag gaattgttcg
    17401 attttgtcag cgaatttgct caagatggat tttatggtga tatcgaaagt tttgagtggg
    17461 caaactatta tgctggacag acatttgatt acgtcttgtt tgcggatgtt ttagaacatt
    17521 tggtagatcc tggaaaaaca ttgaagaaag tcagagaatt tttgaatgaa gaaggagaga
    17581 tcctgattac attccctaac ttggcgcata attctgtcat gatcgatttg ttcaataacc
    17641 aattgccgtg ggcttcttat ggtctcctag atgaaaccca taattctttt tatactcatg
    17701 atggcttcca aaaagttttt gaaaaagcgg gattattcat taatattgaa gattatttgt
    17761 acttagctgt aggtgataca gaattaaaat caacttatga agaattgccg gaagctgtac
    17821 gctatgattt caaaatgcga ccatttggtg aagtctacca atactttttc tcattgatga
    17881 aacatccagt agcacaaagt tccattgcag agccacaaaa ttctaattat gtaaaagtgt
    17941 tagaagtaac ccagcaaacg aaacaagatg aaacaatcca acaaatacca ttcaataatt
    18001 tcacagggga aaatgagaca ctaagtttcc ccgtatcaga aacaacagaa agaatggtgt
    18061 ttcgcttcgc gcaacaacct agctttatcg aattcagcgc agaagctgcc ggagaaaaac
    18121 taacatttat cgattcgaat gctgtagtca aaacagtaaa tgattgttat ttgtttgatg
    18181 ggaaagaatt accagagttc gttctaacgg atatttctgg gaaagaagtg actatccatt
    18241 gtcactatcg ctttatcggg gaattgacac caacaatgaa agaattgttg gaaacgattc
    18301 gtcccatggc tgaagtcaca aagcagcttt ctgagaaaaa tgacgaatta gaaagagaaa
    18361 atcatcatct tcttgaggaa aatacaagac tagaccatac gttaaaaaca accacaaatc
    18421 gctattgcac attgttggaa agtgatgaat ggacaataaa acatagacta ggacgacgta
    18481 agaaagaaac ttcgaaaaaa atccaagaaa aagaactttc tatatgtatt gacgagaaaa
    18541 tctgggatgc agaaacaaaa atattaaaaa tcatcggatg gggaatcgct aattcggatc
    18601 gccagccctt gtcctataag ttatcagctg accaatcgcc atttttcgaa gcaattcctg
    18661 tttcacgaga agaagtcaat caagcagaac agctcgcaaa aggaacagaa gcgggatttg
    18721 agttacgaat cctttgcgag caagaacgtt cattcttagt ggaagcagta gctcaaaatg
    18781 gccaatcatg gatcattgaa atgtaaataa tgattaactt ataaagataa ggagtaaacg
    18841 aatcgtgcaa aaaaaagaaa ttgccgtatt tatcgatcaa atcactcgtg aacgggccac
    18901 tggcgatttg cttattgtgg gctgggcaat tgatgaagtg accaaagaaa tccctacgat
    18961 aaaggtagaa aaagaaaatg tgattgctga agcaacacat gtcgtaagat tggatatcaa
    19021 tcatttatat aatcttgatg ttaagaccca aagcggtttt aaggtacgtt tatcagggaa
    19081 aatgagagga aaagccatct tagacttcca aacagcgaga catcaaaatg gcatagctgt
    19141 gaaattaaac ggtaaatacc cttatgatga cggcattgaa tcttcatggg aaagaaaaaa
    19201 aagattactg aaaaaaggga taaattatgc aaggactcat ggcgtaaaaa aagcgatccg
    19261 tcgggtcaaa ttggaattga agccaggatc gatcgactat gcagagtgga tcagtcgtca
    19321 tgagaaacca gatttgaaag agcaaaggaa agaagcacaa ggatttgcgt accgtccatt
    19381 gatttccata cttatgcctg tctataacgt tgaaatcaaa tggctcgaaa aatgcattga
    19441 ttcggtactg gatcaaacgt atgatcattg ggaactatgt atcagtgatg atgcttctac
    19501 tgatccagct atccgaaagt gtctggaatc ctatcaagca aaagatgata gaatcaaagt
    19561 cgttttccga aaagaaaatg gacacatcag tctggcaact aattctgcat tggaaatagc
    19621 agaaggagaa tttatagcac tcttagataa tgatgatgaa ttaccgccat ttgcattata
    19681 tgaagtggca aaagtgctaa acgtacatcc agaactggat gtgatctaca gtgacgaaga
    19741 taaaatcgat gcagatggca atcgctttga tccgcatttc aaagcagact ggtcgcctga
    19801 tacattgatg ggaaataatt atatctctca cttaggcgta tatcgaacaa gcattgttaa
    19861 agaacttggc ggtttccgta aaggctatga aggttctcaa gattatgatt tggtccttcg
    19921 tgtgacagaa caaatccctg cagatcatat ttaccacatt gaccgagtct tgtatcattg
    19981 gcggacgatc ccaggatcaa ctgcaagtaa tggcgaagcc aaaagttata tctatgattc
    20041 cggagtgaaa gcattgacag atgccctgtc tagaagaaac atcaaaggaa gcgtacgtcc
    20101 tggcagaatt tctggatttt atgagattgc ttatgacgtc ttgcaagagg atttggtcag
    20161 tgtgatcatt ccaacgaaga atggctatga agatctaaaa acttgtgtgg attcgatcat
    20221 cgaaaaaacg tcttattcaa attatgaaat cattattgcg gataacggaa gtacagatcc
    20281 aaaaatgcaa gaattatttg cagaatacaa gcatcagctg aaagaccgct ttatcgtaga
    20341 attgattgac attccattca attattctcg aattaacaat ttagcagcag aaaaagcaaa
    20401 cggcaaatac tttttattct tgaataacga tacagaagtg attgagccag attggatgac
    20461 ggtgatggtt tcttatgcac agtttgatcg aatcggctgt gtaggagcaa aactgtttta
    20521 tccagatgat acaacgcagc atgcaggagt attattagga atcggtggtg ttgcagggca
    20581 tgcattgaat aactatgatc gaacacattg tggttatttc ggccgattgg tcattgacgt
    20641 caattactta gcagtcactg cagcttgtat gatggtcaag gcagctgact tcaatgctgt
    20701 taacgggttt gatgaaacat tagaagtagc attcaacgat gttgacttgt gcttgaaagt
    20761 ttatgaactg ggtcgttaca atgtctatgc acatcaagca gaattgtacc atttcgaatc
    20821 taaatcaaga ggttatgaag atactccaga aaaacaaaga cgttttgctg gtgaaatcaa
    20881 aaaaatgcaa gataaatggc cagcatatat tgcgcatgat ccattttaca atgataattt
    20941 aacaaaagaa ggaatcggcg acttttcgtt gagaccagat taaactataa taaaaagctg
    21001 ttatgttgtg attcgctcgc acataacggc tttttatagt tttttgtcaa aaaaaggcag
    21061 aaggtgccga ctcaaaaaaa taagtcgagg ttgggacaga agtgtttaac tccgagaaat
    21121 aagaagaaat tcacgaaaat tgcttttcaa atttttgtga atttcggctt atttaagaag
    21181 aagttgcttc tgctcccacc gtttattcgt ttttaaagaa tgagacaaaa gtgtttctta
    21241 cttttgtccc acgctcttat tcatgctcag agcgccaata gatacaaaat catagccaaa
    21301 gtttcacgaa ccctagctgt caaagaatcg atattttatt ttaatatagg ctattttaaa
    21361 ataaatggtt taaggtattc ctctacaaaa gccactctat caattatcat gggtttaaaa
    21421 taagaagtga tggagataac cagattgttc ttagcgtcaa tataaagaac atttccacta
    21481 ttaccaattg ctgaatatat ttcgttctca gaatctaata cccaccataa atagccatat
    21541 ctcatatttt gtaagtcttc taaagcactg gttctaggtt tgaacatctc gtttatccac
    21601 atgggtggta cgatttgttt tcctttatac atccctttat ttaggcataa tacaccaatt
    21661 tttgccagat cacttgccga taaacacaaa ccatatcctg ccgtcccgat atttagtgga
    21721 tcacaaaacc agacattggt tttaggagac ttatccattg taaactgctt atgttcttca
    21781 gctgtttcag cataataatt gacatggttt ggtatcccta taggatcaaa tagatactta
    21841 ttggcaaaat caacagttgt tagcttactt gtctgataca acaagccagt taatatgtgt
    21901 aaacagacag tttgatagtt gaattcgccg gttatacctt ttcttcctcc caataaatct
    21961 agtgatgttt ttgtccaatc ttcacttgaa cagacttttg tccatggatc gcctttacat
    22021 ttgtagggtg ctttcattgt tagcaagtgc ttgattgtaa tagcgtaaat agttttctcc
    22081 ccgcgtttca ctttatactc tggaaaataa tctaatactt tatcatcgat acttttgatt
    22141 aatccttgct ctaaagcaat gccaatcagc aaagatacaa tacttttcgt tgctgacatg
    22201 atatgacaag tatcgtcttt tttataacca ttccattcat ctgtataaac ttctacccca
    22261 tccttatatg cgacaatttg acatatattt ggttgtcgaa aaccaataaa atcatgcaac
    22321 tcttgtttat tcataagata ttacctcact ttcttctata agcataatgt cattaaaaat
    22381 cgatttcaat tttttcgatt cttcacgtaa tacaggtatt cttatggact agacagcgct
    22441 ttgggaagat ggattctctg aaaaatataa aggttcactt tatgagtgga taaaaaaagc
    22501 agcaaatact tcgacttctt taatttacca acattagtga aaatcgattc atctggagat
    22561 ttgtaattta gagtccttgt cttgattaga ggtgtataaa gctttttatt agacattttt
    22621 aaattccatt acaatttgct tataaactag gtttttaaag gtgactatgg taagataata
    22681 gagatattat tttgtgatta ggagtgtata tatgaataaa aatggagaat ggagtgctgc
    22741 acgacggata ttcattatat taatggatgt tattgtatac aatgcagcaa tatatagtag
    22801 tttcttactg aagtttcgtg gagagatccc attacgaaat ttagaaactt tccaacactc
    22861 agcaattatt atttcagtga tttttattgc gttgaacatt ttgttgggtg catatgtttt
    22921 ttataatcga atgatcagtg acatcgtgtt tgtcacagtc atcggtcagg tattgatgac
    22981 actagggatc atggtagtga cttttgcggg ccgctggttt gcttttccac gtcttgttat
    23041 cttactttca tttgttctcg gtacgctttt cttaagtatc tatcgtattc tcgtatataa
    23101 actttattta cgcgtaagtc gagataaaaa ggtcgtcatt attgggttag aaaaggatgt
    23161 cgcaccagca atccaaaatt ttggttcaaa gaaaaacaac aaacataaag tcgaagcagt
    23221 cattcttgat aactattatg caaacatcaa aaatatgatt aaagacatcg atattgtcta
    23281 tcttgcttct catatcgaag aaactgaaaa aatcaaaatc tatcaattag ttactaaaaa
    23341 tgaaaaaaaa ttgtttttga atacaacatt tgaaaacttg acattgatca atccaaatat
    23401 tatgaatttc gaagatgaaa gtattattga agcatctgga tttagaatcc aacctgaata
    23461 tgaactattc aaacgtttgt ttgatatcat cgtttcactt gttttattag ttattgcttc
    23521 accatttatg ttattcacag caattttagt caaagtgaca tcaccgggac caatcatcta
    23581 taaacaagtg cggatcacaa aaaatcaaaa ggaattttca atctataaat tccgctcgat
    23641 gaccgccaca gccgaagcaa aatctggacc agttttagct aagagcaatg atgcacgagt
    23701 aacgcctgta ggtaaattta ttcgagctgt ccgctttgat gaattaccac aaatcttcaa
    23761 tgtgctgaaa ggcgatatgt cgatcgtagg accacgtcct gaacgtcctt tcttcgtcga
    23821 tcagttcaat gcagaaaacc cttattacta cttacggcat aatgttcgtg ctggtatcac
    23881 tgggtatgca caagtctatg gaaaatatgt ctctgattac aacagtaaat tacgttttga
    23941 tttactttat atcaaaaaat attcattgat gcttgattta aagatcttgc tgcaaaccat
    24001 caagatccta tttgataaaa tgtcctctca aggacttgaa gaagacgaaa atgtgatctc
    24061 attggcagaa attgcttttc cgaatgaaaa aatctttcgc tagttgggag gcgtaaaaat
    24121 gaacaatccg aaattcagca taatcgtacc agtatacaat gcagaagcaa ctattggtcg
    24181 aacgattgat attctaaaag aattggatta tgaagagtac gaagtcgttt tagtaaatga
    24241 tggatcaaca gatcaaacac aggaaatcat tcaagaactg attgaaaatg atgaacgttt
    24301 tgaattgatt actacgacca atcaagggcc cggattagca agaaacgaag gaataagaca
    24361 gtcaaaagga gaatatctct tattcttcga tgcagatgac acgccagtaa aaacgatctt
    24421 gtcagattat gatcatttgc tagaaaagag tccaaaagcg gatcttgttg ttagttcgtt
    24481 tgtttttcgt acaatggacg agggaagaat cgtttccgaa aaagaaaact tagttacaga
    24541 atgtcactat ctaactcacg aagagtttat aaaagacatg tatcagttaa tgaatgaaca
    24601 gttgatgtat gttgtatgga acaaatgcta tcgtagcgat attctgaaaa ataaccaaat
    24661 tctatttaaa acttataata gttgtgagga tcgaatattt aacttacatt attatgaata
    24721 ctgtcatcaa gtgatcatga acccaaaagt agaatatatt tatgaatttg aaggtgggaa
    24781 ggggatcact aatcaatacc gacctaacaa gttcgaaaca tttaaagaat tctattcatt
    24841 agcaaatact gtaacagacg atattaataa agacggtatg gcggcgcttc ttttaaaagg
    24901 gacaacttca gttatttttt ctatttatag tacctctgca agaacttcaa aagagaagaa
    24961 agacgaagca aagcagatat tgagtgatcc agtgatccga gaagccaagc aaattgctaa
    25021 gacggattct tcagcaaaaa aaataacaaa gttgctttac aatatgccag ctccactatt
    25081 tttatctgct gtaaaagttg gaagcttcgt tgaagtgaaa atgccagggt taatggctgc
    25141 tttgaagcga gtatattagt taatggagga aaatataatg aaaagaattt tgcattttca
    25201 gggacgaatg ggtttaggtg gagcagagtc tttcatgatg aatttatatc gtaaaattga
    25261 tcgtacaaac tatcaatttg attttttgat ttatgaagat tatgcagatg ttcaagacta
    25321 tcattcagag attgaaagat taggaggccg tatttttgtt gtacctaatc ctaagaaaaa
    25381 tatttttaaa tatttaatag aagtgaataa gttactaaaa aaagaatctt tttctatagt
    25441 tcacaatcag gtttattttg gcgggggaat taatttatgg ttagccaaaa agaatggaat
    25501 cagacagaga attgctcata gccatgcaac tgaagatgga aaatctcaaa atattgtaat
    25561 gaatgtattg cgtaaatttt taaccaaatt gttgttacaa aatgcaacag actaccttgc
    25621 cgttagtcaa gaagctggtg aaagtctgtt tcaaaaccac ccctttgaga tagttcataa
    25681 tggtattgat ttagagcttt atagtaaaaa ttcagaagct aaggttaata agagaaaaga
    25741 attggatatc tcaatgagta cttttgttgt gggaaacatt ggacgattag agaaacaaaa
    25801 aaatcaatct tatttgttag agattttttt agaaatacta aaaatgaatt ctgattctta
    25861 cttgatttta gtaggtgaag ggtcacttag aaataaactg aaagccaaag cagtagaatt
    25921 ggatatagag aaaagagtaa aatttttagg agaaagaaac gatattcccg agttattatc
    25981 tgtgatggat gtttttgtaa tgccttctct ttatgaaggt ctgccaatgg ttggattaga
    26041 agcacaagca gcaaaattaa aattagtctt agcagatact atttccaaag atacaaagtt
    26101 aacaaataat gtggattttg tatcattaga tgaaacacca caagaatggg caaaaaaagt
    26161 aataaaagag ccgtatgcta ataaaataac acaagaacta aagagttatg atgtttcgta
    26221 cactactgaa caaatggaga gtatatatag tagagatcat taaaaatgtt caagatattt
    26281 taggagtgat tatttttgaa aatatgttat ttgatattgg catacaatga tattggcaac
    26341 ttaaagagat tggtcaatcg attgaacgaa aatgcagttt ttattattca tatagataaa
    26401 aaaactgata tagaaccatt tattgaagca tttaaatcat atgataatgt tgaattttta
    26461 tgtaataaaa accgtgttaa aat
//
LOCUS       DAYYKN010000026        26427 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000026 DAYYKN010000000
VERSION     DAYYKN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.026"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..2047
                     /gene="tdc"
                     /locus_tag="ACHBB7_000929"
     CDS             212..2047
                     /gene="tdc"
                     /locus_tag="ACHBB7_000929"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM9208896.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2352..3830
                     /locus_tag="ACHBB7_000930"
     CDS             2352..3830
                     /locus_tag="ACHBB7_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9208897.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3796..5127
                     /locus_tag="ACHBB7_000931"
     CDS             3796..5127
                     /locus_tag="ACHBB7_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9208898.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5241..7793
                     /locus_tag="ACHBB7_000932"
     CDS             5241..7793
                     /locus_tag="ACHBB7_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM9208899.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7800..9239)
                     /locus_tag="ACHBB7_000933"
     CDS             complement(7800..9239)
                     /locus_tag="ACHBB7_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9208900.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9214..9396
                     /locus_tag="ACHBB7_000934"
     CDS             9214..9396
                     /locus_tag="ACHBB7_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208901.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9585..9947
                     /locus_tag="ACHBB7_000935"
     CDS             9585..9947
                     /locus_tag="ACHBB7_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM9208902.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10134..10991)
                     /locus_tag="ACHBB7_000936"
     CDS             complement(10134..10991)
                     /locus_tag="ACHBB7_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9208903.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11111..12022
                     /locus_tag="ACHBB7_000937"
     CDS             11111..12022
                     /locus_tag="ACHBB7_000937"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM9208904.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12029..13675
                     /gene="mdcA"
                     /locus_tag="ACHBB7_000938"
     CDS             12029..13675
                     /gene="mdcA"
                     /locus_tag="ACHBB7_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFM9208905.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13777..14076
                     /locus_tag="ACHBB7_000939"
     CDS             13777..14076
                     /locus_tag="ACHBB7_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM9208906.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14066..15742
                     /gene="mdcD"
                     /locus_tag="ACHBB7_000940"
     CDS             14066..15742
                     /gene="mdcD"
                     /locus_tag="ACHBB7_000940"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM9208907.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15746..16339
                     /locus_tag="ACHBB7_000941"
     CDS             15746..16339
                     /locus_tag="ACHBB7_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM9208908.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16363..17439
                     /locus_tag="ACHBB7_000942"
     CDS             16363..17439
                     /locus_tag="ACHBB7_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM9208909.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17464..18333
                     /gene="mdcB"
                     /locus_tag="ACHBB7_000943"
     CDS             17464..18333
                     /gene="mdcB"
                     /locus_tag="ACHBB7_000943"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM9208910.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18444..19208
                     /locus_tag="ACHBB7_000944"
     CDS             18444..19208
                     /locus_tag="ACHBB7_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM9208911.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19697..21790
                     /locus_tag="ACHBB7_000945"
     CDS             19697..21790
                     /locus_tag="ACHBB7_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9208912.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21815..22117
                     /locus_tag="ACHBB7_000946"
     CDS             21815..22117
                     /locus_tag="ACHBB7_000946"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9208913.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            22144..23433
                     /locus_tag="ACHBB7_000947"
     CDS             22144..23433
                     /locus_tag="ACHBB7_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9208914.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            23452..23859
                     /locus_tag="ACHBB7_000948"
     CDS             23452..23859
                     /locus_tag="ACHBB7_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM9208915.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            23882..24751
                     /locus_tag="ACHBB7_000949"
     CDS             23882..24751
                     /locus_tag="ACHBB7_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM9208916.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            24753..25373
                     /locus_tag="ACHBB7_000950"
     CDS             24753..25373
                     /locus_tag="ACHBB7_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9208917.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            25823..>26427
                     /locus_tag="ACHBB7_000951"
     CDS             25823..>26427
                     /locus_tag="ACHBB7_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9208918.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
BASE COUNT         8588 a         4518 c         5654 g         7667 t
ORIGIN      
        1 ttttagaaga ctgatttatt gaaaaattgt tcgctttttt gcgttgaatt atcagaaaaa
       61 tccgacttta tttatttttg tttgtaaaaa tctacgtgga tttgatgtta taaaaaaaaa
      121 attctcattt ttggcgctat agtgaagagg tgttacagat aaacacatgt atctgcaaga
      181 cattgaccaa caaaatatgg agggaagaaa aatgaaagat atggatatca aggccgtctt
      241 tataggcgac aaagctgaaa atggaccagt ttataaaatg ttgttgaaca agatggtaga
      301 tgaacatctt ggatggagag aaaattatat tccttcagat atgcctgcaa ttagcgaagg
      361 agataagctc actccagatt atctagccac ccgcgaccat atgatagagg tcttggatga
      421 agtcagtcag cgattacgcg caggatcgat cccatggcat tcagcaggcc gttactgggg
      481 acaaatgaat gctgaaacat tgatgcctgc tttattagct tacaactatg cgatgctgtg
      541 gaacccaaat aatgttgcat tggaatcttc tatggcaact tcgcaaatgg aagcagaagt
      601 aggacaggat tttgcttcat tattcaatat gacagacgga tggggacaca ttgcagcaga
      661 tggttctatt gctaatcttg aaggattatg gtacgcccgt tgcatcaaat cgattccttt
      721 agctgtcaag gaagtgctgc ctgaaaaagt gaaaaaaatg tctgagtggg aacttttgaa
      781 tttatctgtt gaagaaattt tggaaatgac cgaaagcttt acggacgaag aaatggatga
      841 ggtcaaagca gcttcttccc gtagcggaaa aaatatccaa agattaggga aatggctggt
      901 ccctcaaaca aaacactatt cttggatgaa ggcgttagat atttgcggag tcggcttaga
      961 tcagatggtt gctatcccag ttcaagagga ttatcgaatg gatattaatg ctttggaaaa
     1021 aacaattcgt gaattagctg gacaaaaaat cccaattcta ggtgtggttg ctgtcgtagg
     1081 aacgacagaa gaaggtcaag tagacagtgt agataaaatc gtccaattaa gagaaaggtt
     1141 aaaagatgaa gggatctatt tctatttaca tgttgatgcc gcatatggcg gctatgcgcg
     1201 ttcactgttt ctaaacgaag caggagaatt tgtgccgtat gcttccttag ctgaattctt
     1261 tgaagaacat catgttttcc accactgcgt aacgattgac aaagaggtat atgaagggtt
     1321 cagagctatt tcagaagcgg attctgtcac gatagatcct cataagatgg gctatgtccc
     1381 ttatgctgct ggaggaatcg taatcaaaca taaaaatatg agaaatatca tttcttattt
     1441 cgcgccatat gtgtttgaaa aatcagtaaa ggctccagat atgttaggag cttatatttt
     1501 ggaaggatca aaagctggag caaccgccgc cgcagtctgg acagcacatc gtgtattgcc
     1561 attgaatgtg accggatatg gtcaattgat tggggcttca atcgaggcag ctcaaagatt
     1621 cagagaattt ctcgatcatc tgacttttac tgttaaagga aaaacaatcg aagtctatcc
     1681 attgaatcat cctgatttta atatggtcaa ttgggtattc aaagaacaag gctgtacaga
     1741 tttgaacgct atcaatgaat taaatgaaaa aatgttcgat cggtcttctt atatggatgg
     1801 cgatgtttat ggtgaacggt ttattacttc tcatactaca tttacacaag aagattacgg
     1861 tgattctccg attcgttttg ttgaaagaat gggtctaacg aaagaagaat ggaaaaaaga
     1921 acagaaaatc actctgctac gtgcggctat catgactcct tatttgaatg atgatcgaat
     1981 tttcaatttt tatacaaaaa aaatcgccaa agcaatggaa aagaaactaa acgaaatcat
     2041 ccaatagaac tgatttttgt gtgaaagaat aacaagagac atatggagaa cagacaatta
     2101 tttgcttcat caaaacaatt gtatttcccc ctgtttttca tatattcctc gccaagatat
     2161 tcttttttat aaaacgttct ataagttgga tcgttcgttt catgtatcaa acccagttca
     2221 ctaagatcgt tcaatggatg tttcggtgat actgggtttt gtactgaatc gatgaaacct
     2281 tgtcatttct gttcttgaac aaaaataaaa gaaaacaaaa acatatgtcg ctattgaaag
     2341 aaggaataaa gatgaaagat actggttcat caaagcaaat cacgaccatg acctttatcg
     2401 gaatgacctg cgcccttgta gcaagtgtac gaaatattcc agatgtagca gcaacgagct
     2461 ggacgatgat tttttacatg ctggttgcag tattattgta cgcttttcca atctcattga
     2521 tctctggtga atttgctgga atgtttcccc aaaaaggtgg tccagaactt tgggtatcga
     2581 atgctctagg aaaaaaatgg gggttcgttg tctcatggct gttatgggtc caaatgtttc
     2641 ctgggatggt gatggtcgct tcagcactcg ctccgttgtt tggtaatatt atcgataatg
     2701 tgccgcttgg tttgaacagc aaatttacat tagttgtcat tttagtcgtt tattggatca
     2761 tcactttttt gaatttgaaa tttgatatgg caaaaatcgg cggaaaagtc ggtgtttggt
     2821 taggattgta cgtgcctttg acgatgatgc tcttattagg gtttgctgcg tggatcaaag
     2881 ttgggatcgt accaaccgga acattaggca gtttttcatg ggacaaatta ataccagata
     2941 ctacgactgc atcctcattt gtttattttg cgccgatcat gtttattttt actggtatcg
     3001 aaatgtcctc tgtgtatatc actcgattgg aaaatccagt taaaacgtat attcgcggag
     3061 tatttgccgc gttaattttt atatttttcg tcaatatttt gaatgcttta gttgtagcaa
     3121 atgtcgtacc aaaaggacaa atggaattga ataatatcgc gcaatctatt tcgatttatt
     3181 gccaaattct tggtttgcca catcttattg tgaatctgtt tagtttactt gtcttcattg
     3241 gtgtagcagt ccaattatct gcttgggcct caggaccagc aaagacagtg acagaaagtg
     3301 ctagaaaagg tgcgtatcca ccaaaattca atttttggaa gacgaatcaa tttgacgtat
     3361 ccaaatccgt tattttgact cagtctgtca ttatctcaat ctttgcactt ttttatttgc
     3421 tgattcctgg cgtcaatcaa gcatttttga tgctcgtaaa ttcaactact gttatctact
     3481 gtatcgtcta tgtcatcatg gggatcgcag ttttgagatt gcgttataca catgcaaagt
     3541 tgaatcgacc atttagaatt ggaaaaaaag agaaaaagag caatcttgga gtttggactg
     3601 tcgttattgt tttatttgcg gcaatcggat tttctgtcgg actgacgatg aaagctggaa
     3661 catggatcaa tctaattgca gtcacagcca tcagtgtcat tttgtttgta gtaccactct
     3721 gtattgaaaa aataaagaaa ccttcttggg aacaggaagt cgccatgctg cttgaaaaag
     3781 aaaaaggtga acttgatgga aaaaccggca atgaaacgga ctctaactaa tcgaaatatc
     3841 cagttaatcg cattaggtgg cgcaattgga acaggattat ttttaggttc agcaaaggca
     3901 attgcatcag cagggccagc tgttattctc gtttatttga tatgcggttt ggctatttat
     3961 ggtttgatgc gggccatggg tgatttattt ttagcaaata gtacatttaa ctctatcagt
     4021 gatttcgttg actattattt aggcaaaaag tgggcatttt ttgtcaattg gacttattgg
     4081 ctttgctggc ttggagctgg acttgcggaa ttaacagcaa taggattgta tatccatttc
     4141 tggtttgtac atgtaccagc tttgataagc ggaggtagcg cactaatgat cttaatggca
     4201 gtcaaccttg ttgcagtcaa gtggtttggt gaattggaat cttttttctc aatcatcaag
     4261 atcttgggga ttttcctttt tatcttattg ggtataggat tgagtgtctc agattttcat
     4321 acccagtcta tcggaatcaa agaaaatttt ggacagatcg atgaattact accgtttggc
     4381 tttgctggaa tggtagtctc atttcctatg gttttattca gttttgctgg aattgaaatg
     4441 atcggcttaa ccgttggcga gacaagtgat ccgcaaaaga atttgaaaaa agcaatcaac
     4501 tgtctgcctt ggcgtattct ctttttctat ataggaacga ttttgtcgat tttatttgtt
     4561 gttccatggc attgtttaga tttaaaagaa agtcctttag tgactatgct gcaagtgatc
     4621 cattgccaag gcggagcagc aattgtcaat ctaatgattt tggttgcttc tttgtcctca
     4681 gcaaatagtg cagtctacag tactagcagg attttatatc aaacagcaaa agaaaaaagt
     4741 ttgccccatt ggttttgtgc gctttctaag aaaggcgtac ctttacacgg tattttaatt
     4801 acaggaatgt tattttttgc cggtctttgt ctacattttt ttgtagctga tagtcgattg
     4861 ttttttcagc ttttggcagg catgacaacg agttgttttc tatttgtgtg gtcttcgatc
     4921 ttgtgtgcac atgttcaatt tattaaagaa aagaaacgga acactacaca aagatataaa
     4981 gatcttgctt tgttggtgtt ttttacttca attgctttca gcttgttatt cgaaagcacg
     5041 acacgcttta ttcctttttt attggttatc tggtttgtat tcttaaattt tgtatataaa
     5101 agaataagca atagaaatat cagttaggcc cagatacatt ttgcaaaacg aattatttca
     5161 gacccccctg aaataatcga tacgcctgaa aaaacttccc tgcgtataat aacagtatca
     5221 attaaacagg aggggatagg atgaactggt acagattgac ggtagaagat gtattgaaaa
     5281 actatcaaac atcaacagaa ggcttgtcgc aagaagaacg acagaaacgc ctgactgatc
     5341 aaggatataa tgaaataaaa gcaaggcagc agacaaaacg atggaaaaaa atagcaaagc
     5401 attttactga tctgctgatg atcgtgttga tcattgcctc ttttttgaaa tttgcttcta
     5461 gtgaatatat tgaaggaagt attattttat tcgtagtgat cgtcaatgga ctcgttagtt
     5521 actggcaaga aagaaaagcg gaagaatcat tgaatggttt gaagcagctg atgggacaag
     5581 aagcgatagt actatcaaac gggatgcaag aaaaaatccc tgcgaaagaa ctggtaaaag
     5641 gagatgtcat tgtcttacgt gccggagatg tcgttccagc agatatccga ttaatcgaag
     5701 cacatgattt attagtggaa gaatcgattt tgacgggaga atcagaagca tctgaaaaaa
     5761 gtcatgccgt tttgacagaa gaagaatcga tcggcgacca gaaaaatatg ggtttttcag
     5821 gaacgcttgt acaatctggt tctgcagtcg gtgtcgttgt agaaacagga aacacgacag
     5881 aaatcggtaa aatcaatcaa gcattgcaat cggtcgaaca acaaacgacg cctctgatta
     5941 aaaaaatcaa ccgattaaac aaacaaattt ttcaaggcat tctttgtttg agcctattct
     6001 tagttatttt tacgacattt cgatatggta tggattggca tattcttctt tcggctacga
     6061 ttgctttggt ggtgtcaatg gtacccgaag gattgccagc tgttttgacc atgattctat
     6121 ccgttggagt acatgaaatg gcaaaagaaa aggcgatcat caaaggactt ccttcggtag
     6181 aaacattagg ttctatgaca gtgatctgtt cagataagac aggaacattg acaaaaaatg
     6241 aaatgacagt tgtggatgta gctgcaaaag aagaagcctg cgtgttgtcc attatgaaaa
     6301 actgtcagga attaaaaaca aaagaagaac agaaaacaga aaatttaagt ggcaatccga
     6361 cagaagttgc gctgattcga cacacagaaa cagccaatct acctttgaaa aaaatagaag
     6421 caaagattcc gttcagttct gaatacaaat atatggcaac gatgcatgcg gaggaaaaag
     6481 gagcggtgat ttacgtcaaa ggtgcaccag aagtattatt tgctaaatca actctttctt
     6541 ctgcagaaca agaagcatgg agtcagacag ctgcagaatt tgcgagaaaa ggtcagcgtg
     6601 ttttaggatt tgcttataaa aaagtagatt cgaaacagga actcacgcat gagactttaa
     6661 cccaattaac ttttgcaggg atcgccggat tgatcgatcc gccaaaagaa agtgcagtca
     6721 aagctgtaaa ggaatgccaa caagcaggga tctcagtgaa aatgattaca ggggatcaca
     6781 aagacacagc aaaagcgatt gcggaacaaa ttggcttaaa acatactgcc aaagttttag
     6841 aagggatcga tttggatctt atgtctgatg aagagttgat tcagcaagtg ccaatagtcg
     6901 atgtctttgc tcgaacgaca ccagaacata aactgcgaat tgttaaagct ttgcagaaaa
     6961 atggagaaat cgtcggaatg accggagacg gcgtaaatga tgcacctgct ttgaaaagat
     7021 ccgatgtagg aattgctatg ggaatcaaag gtagtgaagt aagcaaacag gcagctgata
     7081 tggttttagg agatgataat ttccatacga ttgcaaaagc cgtaaaagag ggccggcgga
     7141 ttttggataa tttgcaaaaa accatcaatt tcttcttgcc aactgcatta gctcaaggat
     7201 tgatcttgat ttgggcactt atgctgaatc gcccgctacc tttgtcccca gtacagattt
     7261 tatgggtcaa tatggtaacg acaatcactt tatcttatgc actagggttc gagtcgccag
     7321 atccagaaat catgaagcgc ccaccaagag atccaaagca aggaatccta tctggttatc
     7381 atttgtttcg gatcatctat gtctccctac tgatcatggt tcctgcctat ttattggcta
     7441 tgcagtttga agggcaaagc ttacagcaaa ccattcttct gcaaaatatc gtcttagctc
     7501 aagctgttta catgatcaat tgcagagagt tgtccaagcc gtcattgaac caaggtcttt
     7561 tgcaaaacaa agcgcttttt ctttcattag gtatccttgc tttattgcaa gggatggtta
     7621 tttttctacc agtcggccag cagctgatcg gtacagtaag tttaacgatg acacaacagt
     7681 tattgattgg agcaaatgtg attcttctat tcttagttgt agaaatagaa aaagggatca
     7741 tgaacaaact gtttcgcaaa aaagaaaaaa cagctagaga atacagtcgc tagccgttac
     7801 tataaaaaag ttttgaaagt gagttcagtc aaacgattga gctcattttt cgttggaata
     7861 gaagaaatgt atacaatagg gatatctaga gaatctagag ggaaattgga aatcaagata
     7921 tctgtttttt caaaagaaat agtggaaaga tcggttagtt cgataggtaa gaagcagaca
     7981 cgttttccta agtaatcttt taattcctgc tgcaagaacg ccttccatga aggttctcct
     8041 tggaaaataa aatagacatt gaacaaattc ggtttgatag aaaaaatcgt ttgttggatc
     8101 agtagagaca agagttggag aaagtaattt ggttcattga tttctatttc tttttttaga
     8161 tcgttcagta aattctcttt ctgttgatac aagtgtgcat attgctgatc atatgtttct
     8221 aaaaaacgat ggtattgacg gtccaatgct tcaatcaata tcggcgattc atgatatttt
     8281 aatatgagga gagccaagtt atcagtcaga gatgtttttt ttagttgatc aagagacagc
     8341 tctgaagcta gttcctctac aaacgtttcg aggacctcta aaaatggaga atgatgcagg
     8401 cagcgagcgg tttcttgtcc atagttattg ttgtaattcc aactttccag aaagcagaaa
     8461 gaagcaaaag aaagctcagt gtctttaaga taaatacctt ccgttaaata caattttttc
     8521 aatgcagagt catacaggga ggagagtgtg ctgttggtat ggatagaaga atctaatcgg
     8581 caattcgctt ttatacggat cgtattgata aagaaccaac atgttccgaa aatctcttct
     8641 gtttcaaccg ctagttcagt ttgactgagg tttcttaaaa attgaaaata atgggattca
     8701 tggatcgaat aatcctcgaa aaaatagttg ttatgagtga aaggaatcag caatcgatga
     8761 taaaaagtac gaatcgcacg ctcttttcca agaatacaag ctgtatattt cgtcaaatcg
     8821 atctgcaagt cgtaacttgc taaacgctga ttgattttag tcacatgtct tttcaatgtc
     8881 ccataagaaa gcccatggtc ggctagaaaa tcagggacag caactttttt tgtaaaaagc
     8941 agatttttca gcagttcgac actgactgat tgtttgaaaa aaatctccca aaggtcattt
     9001 gttaataaat tttgctggtt ttgcaaactg attcctgctt gatcgctgat gatgtcccag
     9061 ccttcgggca gctgagagcg aatagattga aggtcagaaa aaatcgttcg ttcggttgtc
     9121 cctatgattt cagctaattc atttacggtt atctttgtat gattcagcaa ttgttctact
     9181 aaagaaacat gacgtcgaat atctttttcg gtaatgattt ttttcattag tgaatacatc
     9241 aatcagactc ccttctattc taagaatacg gagaaaataa acggtggtca agtttcaact
     9301 gatttttcaa tcattgttta tttttctgga gcatttactg atgataagaa tttcatcatg
     9361 aacttgaaaa atgataccac taaaatcaaa aaataggtgg gcaaacaata ataaacattt
     9421 taatagtaga tggtagcaag accaacacag gcggattgaa catttaagag ttgataaaaa
     9481 agaaaaatat cgaagtattt gattagcaac tacttgtatt catttttcta ttcttacgtg
     9541 gtatctttga ataaagcgaa tcaataagaa agagatggaa gcagatgcta acgaaaaaag
     9601 aactaatcga tcacatcaat gaaacataca atttgtctcc agaatatatt tttaaaaaat
     9661 ttcctgatta ttgcgtattc agacatcaaa aaagcaaaaa atggtttggg gtcctgatgt
     9721 ctgttccaga agaaaaaatc tatgatgatg caagtgacca taggatcatc aatattcttg
     9781 atttgaaggt cgatcctgaa cttggcggaa ttttgcgagg aaaagagggg tattttccag
     9841 cctatcacat gaataaagaa cattggatct ctattgatct acgtaaaata gaaaagctag
     9901 atgaactagc agatttgatt gatggaagtt ttaaatcaac gcaatgaatg tctaggacaa
     9961 tagagaagag gttgtgacag aaatgtttag cttcaagaaa taagaaggaa tttacgaaaa
    10021 ttgcttctca aatttttggg aaatttcggc ttattttcta agaaattact tctggaacac
    10081 cgtttgttcg aaaaaagagc tgcgacaaga attttgtccc agctcttttt tttttatttt
    10141 cctaaataaa attcccgcat cgtttttgca atcattttga tcaaaggatg atcttctgtg
    10201 aagctagaag ataaataaat tgttcgtgtc aagggcaaat ctttgatttc ccgcaagacg
    10261 aaaggatcat ttttatcgta agaccaagaa acttttggga aaaagctaac agctaatcct
    10321 tgcttgacaa tagaacgtaa tgtcgcagga tcatctgtcg tcgaactgat cgtcaattcg
    10381 atacttttcg cttcaagtgc tgcatccagt gtatgacgga aggcgttgct ctttgacagc
    10441 atcaagatgg gggtgcgttg gatatcttct aattgcagat gttctttttt agcaagagga
    10501 tgtttcttag gaatggcgac ataaacaggt tctttcatga taggaatatt ggtcatgcca
    10561 ggcttcatct ccgaagtgat gtacagtaca ggatcggtaa tatcatctgg taaatgatgt
    10621 tgcatgatcg ttaattggat atcaggatgc ttttccataa aaattcggac caagttcgga
    10681 atagaaacag aggctacctc taaatacaat atgacggaag taacgatctt ctcattgacc
    10741 gaagacaata atttgattcc ttcttctaat gtgtctaatg tcttgactgc atatggataa
    10801 aattcttctg cataaacggt cgtctcaatg tttctgccgc tatggtaaaa caaagggaca
    10861 cctaattcgt tttccaaatg tttgatcgca cggctcaagg caggttgaga aataaataat
    10921 tctttggctg cttgggtaac attttttgtt tcgaatactt ttttaaaata ctgcagatga
    10981 agcaatttca taattatcca tcccatccat acctttttgt tatggttttt tctttttttg
    11041 gcatttttta ttatagcaga aattgactaa gatagcagtg gaaaaagtaa gtgctgagga
    11101 ggcgaggctc atgagtacag gtttcttatt tcctggacaa ggcagtcaga agttagggat
    11161 gttagcggat atccctgaaa tgtacatgga acgtttagaa aatgtgacgg ggtatcggtt
    11221 agttgataaa gaagaaaact atcaagatac ggtttttatc cagctagcat tactgacaaa
    11281 agcagtattt tatttagatg aaatggaaaa aaacggtatt cagcctgata ttgcagcagg
    11341 acattcgatt ggtgcttttt gtgcagctgt tgcttgtcga tctttgagat ttgagaatgc
    11401 agcggaacta gtctatcacc gcgcagtctt aatgaaagaa gcttatccaa gcggttatgc
    11461 aatgggcgta gtagtaggtg tgacgagaag cgaagctgag gaaattgtag caaaaacatt
    11521 tgatcctgac catccagttt atctgtcaaa tgaaaattgt ccgatgcaac atacgttatc
    11581 tgggagtatc gaaggtttat ccgttacgtt ggcagaagcc aaacggcacc aagcgcaaac
    11641 agcgaagctg ctgaaagtcc ctgtgccatc tcactgtctc ttgatgaatg atacggtcaa
    11701 gaaattcaag ccgtatatgc agtctgtcca tctatctgat gctagatgtt tgtatataaa
    11761 aaatacggat ggaagagcca ctcaggacgc agaagagatt cgtaaagatt tgttggaaaa
    11821 cctagctttc cctgtgcagt ggaaccaaat gatggacttg gcaaaagaat taggaatgga
    11881 tctgacgatt gaatttcccc ccggcaatac tttgacaaag ctgattcata caaaatttgg
    11941 tgtagataaa gggatccgta cgatcaatct agaccaacat ggtattgatg acgccacgtt
    12001 tctatatcaa aaatggagat gactaattat ggaaaaacca aaccgcaact gggcttctaa
    12061 acgcgaaaaa aaagcgcagc gattggaaaa aatcagcaac ttgctcaatg gcaagtatgt
    12121 agaaactgaa aaaatcgtag aagtaatgga aaaactgatt gctcccggcg acaaagttgt
    12181 attagaaggc gacaatcaaa aacaagcaag ttttctttca gaaagtcttg aaaaagtaga
    12241 tgcagaaaaa gtgaatgatc tgcatttgat catgtctagt atctcacgac cagaacattt
    12301 gaatattttt gaaaaaggaa tcgcaagcaa gatcgatttc tcttatgctg gtgcacaaag
    12361 cttgcgtgtc gcccaaatga ttgaagacgg cacaatgaaa ttaggtgata tccatactta
    12421 tttggaactt tatggacgct tattcatcga tttgattcca aatgttgttt tagtagctgc
    12481 agataaagca gataaagacg gcaacttgta cacaggtttc aatacggaag aaacaccaac
    12541 aatcatcgaa gctgcagctt tcaaagatgg tatcgtgatc gttcaagtta acgaagttgt
    12601 cgattcttta ccacgtgtag atatccctgg tgactgggta gacgttgtcg tgaaagcaga
    12661 taaaccttat caattagaag ctttattcac acgagatcct caaaacatca cagaattgca
    12721 aattctaatg gcaatgatgg caatcaaagg aatctacgca gaacacggcg tacaatcttt
    12781 gaatcacggt attggattca atacagcagc tatcgaattg ttactgccaa cttatggtga
    12841 atcactaggg ttaaaaggga aaatcgcgaa gaactgggta ttgaacccac atccaacgat
    12901 gattccagcc atcgaatcag gttgggttga aagcatccat agttttggtg gcgaagttgg
    12961 tatggaaaaa tatattgctg ctcgcccaga tgtctttttt gtcggtaaag acggcacgat
    13021 gcgcagtaac cgaacattat cacaagctgc aggacaatat gccatcgata tgtttatcgg
    13081 ttcaaccttg caattagatt acgaaggaaa ctcttctacc gtaacaaaag gacgtttatc
    13141 tggttttggc ggtgcgccta acatgggaca taatcctggc ggtcgtcgtc attctacacc
    13201 agcatggcaa tcacttcgcg acaatgatga tccattaggg aaaggccaaa aattagttgt
    13261 tcaaatggtt gaaacatatg gttcaaataa aaaaccagtc tttgtcgaag aattagatgc
    13321 actagctgtt caaaaacaag caggcttagc caatgcacca gtgatgattt atagcgaaga
    13381 tactacacat atcgtgacag aagaaggaat tgcttatttg tacaaagcaa aaacaaaaga
    13441 agaacgacaa gcagcaattg cagcaattgg cggtgttaca cctcttggaa tgactagcac
    13501 gaaagaatca ctagatagct tgcgtgcagc cggcatcgtg aaattaccag aagatctagg
    13561 catcaaacgt agtgaagcga aacgttcatt attagctgca caaaccatcg atgatttgat
    13621 cgaatggtca gatggcctgt atgaaccacc aatgaaattc agaacttggt cataaaattg
    13681 gcgagaaaaa ccttttgtcg ccatttaccc agatggaaga agaaagacga ataagcagaa
    13741 cgtgtatgct ctgttttaca aaaaggggag agatccatgg aacaattagc ttataaatat
    13801 ccaacgaaac agccgatcaa taagaaaatc catgtaggag tggtcggttc aggagatttg
    13861 gaaatcttga tgtttccaac agaaaaaaca gaatctactg taaaaatttg tacaggaagc
    13921 gatggcttcg gcgaagtatg gaacaatgta ttaacgagat tttttgatcg ttatccaatt
    13981 tcagcagata tcgtgatcaa tgacttcggt gctacaccag gagttgtgta cctacgattg
    14041 acgcaagcaa tggaggaact ttcagatgaa aaatagtttt gtagaattaa acgccagaga
    14101 acgagtgaac gctctacttg acaatggacg tgaattgatt gatccattcg atcaaatcat
    14161 cgcgccaagt ttaccagcac aaggaatcgt ccctgaaagt gatgacggga tcgttgtcag
    14221 ccgtggcacg atcaatggca aacaagcagt agtaatcgct atggaaggaa aattccaagg
    14281 tggcggtatc ggtgaagttt ccggtgcaaa aatcgttgct gcacttgaac atgtgttaga
    14341 agaaaataaa aaaggaaatg agatctttcc agtcattgta ctagatacag gtggtgttcg
    14401 tttgcaagaa gcaaactacg gactgttatc tatttcagaa atcggaaatg taatcattgc
    14461 tctaaaacaa tatgtaccag tcatcggttt gattccagga cgtgtaggat catttggcgg
    14521 tatgtcgatt acttcagcat taatgagtta tttaatcgca acaaaaaaag cgagagtcgg
    14581 tttgaatgga ccagaagtta ttgaacaaga agcaggcgtt cgagaatttg attctagtga
    14641 caaagacttg atttggaata cattaggatc tcgtcaacgg gtagaatcaa aaatcattga
    14701 tgaattagtg actgatagtg tagaagcaat caaagctgct gtcatcgcag caatggacga
    14761 acgaaaagac acgcatcgca gtgaaagctc agcattttat ttatcattac ttgattcttt
    14821 agatctttca aaaccaatgg ccattgacga ttacaatgaa gcaattgcta accatcaagc
    14881 aaaatcaatt gacttaccag aagtagcaga aggaaatgaa ccagctgcag caagtgttgg
    14941 ttacgattgg tttgcggcac taactggcat gaaaaatcca acttcttcta ttagtactgt
    15001 gtattatggt aaatcaagta aattcataga agaagccatc gtgacatcaa tcgtacctga
    15061 tcctaaaaac ccgatgtacc gtgtacgaaa cggtgaagtt ggtttagttg aaggtttccg
    15121 catgggacaa attttggatc acgtttacga agaagataaa gacaaaaaag tgaaacggcc
    15181 aatcatcgtt gtgatcgatg taccaagcca agcatacggc tataacgaag aattgatcgg
    15241 tatccatgtg gctttggcta atagtgctgc tgcttatgca aaactccgtc aagctggaca
    15301 tccagttatt ggattgattg caggaaatgc tatttcaggt gcatttttag cacatggatt
    15361 acaatcaagc cgcttgattg ctttaaacaa taaagaaatt accgtccaag cgatgtctaa
    15421 agaaagtgct gcacgtgtga caaaacgttc ggttgctgaa atcgaagcag ctgctgaaaa
    15481 agttccagct atcgcttacg atatcaataa ttatacaaaa ctaggagcga tctatcattt
    15541 cctagaagat atcaccgacc aagctgcttc agaacaaaat gtcagcaaag tcatcgaagc
    15601 aatcaatgaa gccattcaag atgtccgcaa tacaaatgac acgatgctga caaatcgata
    15661 cacaaacgaa acagccaaaa cattcggacg tgtcgaaaca aataaagtcc gtgccaaaat
    15721 ggacgaagaa tgggatgcat aaaaaatgat tcaagcacat gatattgtcc tttttgataa
    15781 acatgaaata gaagggttca ccttgcctga atgggtcgtt ctaactgaaa agatgttcgg
    15841 aacagttcgt cgtgcgtcga cggatcacgc agaaatacta ccgattggtt tacgtggacc
    15901 tgcccgcaat cagagatggt caacagaaat ctcaaaacat gccgtcaaac aagtcattca
    15961 tccgtgggac atcatcgaac aagaatcgta tagacaagag aaaatcatgg attatcctgt
    16021 ttatcagcaa tttcatgctg ctagaaacat attatcagga tgtaaatggg gaatcggcgg
    16081 cagcttagga tttgaattat cgactgggat tccggcagta aaagaaacca gtgattttga
    16141 tttgctgctg tacgcaaatt caccgattga attgccaatt caggtaattc aatcgcatcc
    16201 tgcttttttt gagcaattcg atacccaagt agtgactcgg caaggcggtt tttcactaaa
    16261 agagtatttg agagagccag agaaaaaaat tctattgaaa acagtcgaag gacctaaatt
    16321 aaccaaagaa ctttggtaat aaagaaaaag agagagaaag agatgtttga aaacaaaaaa
    16381 atcaaaatca acgtaagtga cttccagaaa gtatcagctg taacggctgt tatgatgcaa
    16441 acgatattga gctttgcttt ggcaaacagc cacagcgcaa caaatacagc ttttcttggt
    16501 accttttacg tcatcgttaa atacacagca ccaatgttta tttttgcaat tgtttataat
    16561 atggtaaaaa caagtcagca tttgtcttat tgggaatttt tgaaagaaaa gttttttgaa
    16621 ctagtcgttc cgtatcttct atggacgacg gcttatcttt ggctatttcc agcagtccaa
    16681 caacaaactc cgtatacgga tactgcaagc tttttactga aatatgtcac aggtgatgga
    16741 gcgcctcatt tatggtatac cgttatgatg ctgcagatcc aattgttcat gccgtttttt
    16801 gtttggctcg gttacaaagt atttgctaat aaaaaatatg tatggccagt attgattggt
    16861 tcaacgattt tatatgtcgc atggtatctg ttctatcaaa cacaagtatt gactgggcct
    16921 tatcatgaca gctggtactt attagatcgg tttgttgcta gtttcatgat ttatggggta
    16981 gccgcatttg tttatcacga aaaagtctat caatatttgg acagagtacg ttatcttttc
    17041 ttacctgttg ccttagttat cgcgttcttt tctgtccgtt cattgctcgc acatccagga
    17101 gacttgagct ttgcgaatgc accttacttg aatacgatcc aaagtttata cagtttagtc
    17161 atcatctttg ctgtcttcat aggcgcttcc aaaatgattg taaacgattc gccaaaattg
    17221 ccattgttca aatggttgtc tgtttatgct tatcgtacgt atttagccaa tgtatttgtt
    17281 ttccaagtac tgttattatt gtttaaagat agttggctgc agttaccttc aggagtcatg
    17341 attcttgtag cttatctaat gacggcaagt tgtgcatttg ctttaagctg gttacttcat
    17401 atcatttggg tagctatcaa aaaaggtttc agcaagtaat agtaggaaga aggagtgagt
    17461 gtcatgataa atgacgaacg aaaaaaagca gtgactcagc aactcgctgc gttggtagag
    17521 gaagcattga tcgcagaagt aacattaagt ccaaaacctg gattagtgga tgcactggat
    17581 gcgggtgcac attccgatat ggactatgct ttatttctta agtctgctaa gacgctaact
    17641 cctttttttg aagaaatggc tcaaatagca tggcatcatg ctattgatca ggagttaaga
    17701 gaaagaattg ctgagattgg gcgaaaagcg gaaaaggcaa tgctgattgc tacaaatggc
    17761 atcaataccc ataaaggagc gatttgggca atggggctat tgatcagtgt cattgcacaa
    17821 aaaatcagta tggaacagcc tttttcttta tcgaaaatac ttgatgatgt cagcaagctt
    17881 gctgcttttc ctgatacaaa atatcgtcat aaaaaaacca ctcatggtca tagggtgaag
    17941 caaaaatatg gcgtaaatgg tgcatatgaa gaagcgatat tagggtatcc gcatatcagg
    18001 cttgcaattc aagcagctaa ggagctagat aatgattcat atgtacagaa acaaatgcat
    18061 atgttgttag ttttgatgag ttcgctagat gacacatgcg tattgcatcg cagtgatcag
    18121 aaaacgttag tggaaatgca aaaacttgct catgctgcta gtcaaagtgc tttaccaaat
    18181 cgtccattta cagcactgtt gcacttttgt caggaaaacc aaatctctcc tggaggaagc
    18241 gcagatctct tgtctgcaag cttgttcatg ctgaagatag aggaattatg gactgccgac
    18301 acatacattt gtccgatgtt actcaatgac taaataagac aaaattgcta aaaaaacagt
    18361 ttatgcatct ggttattgac tcttcctttt agcaaggcta tcataaaaat agttttttgt
    18421 aataaaagat aacgaggaag acaatgaaaa aaatcaatta tttagcaatc attttgttag
    18481 tgggcctatg tttgtttgct ctgccgaccc aaggattcgc tgtcgcgggt ggtcatggtg
    18541 gaggtacatc tagcggtcga agttcgggca gaggttcatt tggtggtcgt tcatatgggg
    18601 gcagttattt tggcagtcgt tcttacggca gagattctta tgggtattat ggtccaggta
    18661 caggtccttc tagttatgag caaacgggat ttcttgcatt agctgttttg tcagtaggag
    18721 gggtttctgc tttttctatc gtcaagaata aaagacaaaa gaaaaggctg aaagcggaga
    18781 tattgtcccg catgtcagga acggctaaag aaaagaaagc acagtttctg gaaatccaaa
    18841 agatattcat gaagatccaa tccgcttggg aagaagaaac tcctgaaaaa gccagaagct
    18901 gttatacaga caagctgttt gccgaacatc agaaagttct gcaaaaaaac aaagcagacg
    18961 gtgtaagaaa tcatactaaa aaaataacta ttcaggaagt aggaaattat cgacagatcc
    19021 acgaaaatag ttttgccatc cgaattgatt tcagatgctg tgattattca gtcgatcgaa
    19081 aagatgggcg attgatctca ggaagtaaga cacaacgaca gtatttttct cagatatggt
    19141 attttgattt ttctgagaaa gaaaaaagat ggcaagccga ttttattcag ccgattatgc
    19201 tggattgaca ggaaatattt atgaacggag ttgtgacaaa agccttgtcg cagctccttt
    19261 ttctgaataa acggtgtgcc agagatagcc tatcggcaat aaggcacgat tttgtaaaat
    19321 atgggaagcg atttttcaaa atcgcttctt atcgctcaag gctgaacatc tctgtcacaa
    19381 cctctccact aaatggtgtt taaaagcttg catctgcgat aataagaaca accaaacgaa
    19441 aaataggaag cactatttta gtttagttgt tcttattact tgatgcagga cagctttttc
    19501 acaacctctt ttttgtatag atgggcaaaa tttttgtaat aataggcgta tgtctttttc
    19561 gttgaggaga tgaaaaagca tatggcttat tttttgtttg ctttgcttac tttctttcca
    19621 cttcaagtgg cagaaatatc tactttttat tatgtaagcg gaaccatata cttgaaatca
    19681 gaaggagatc atttctatga aaacaagaac aattgaaata cttcacagaa tcatgacagc
    19741 ccaatatcca gtaaaagcaa aacatctagc aagtgagttc aatgtcagtc agcggacgat
    19801 ccgtcaggaa gttcttgaag cgaatatctg gttgagcagt aaaaagcttc cggagatccg
    19861 tacaattcgc aacaaaggtt ttttgttgaa gctggaaaag gaaaagcaag ctcgattaga
    19921 aactgaaata aaagatgtga aagacggctt tttgaatcgg cgcgaacgta tttttgatct
    19981 agttttagcc gttgcttaca gccgaatccc gatccatcta aacaaaaaag aagaagaatt
    20041 tcaaatctct aaaagtacat tagatgaaga tatgagacgg ttacgagcaa cactggtcag
    20101 ttatgggata gagatcgtca gttatggaaa gcaaggactg gtttataaag gttcagaacg
    20161 atctatccgt acgatgatct atgattttat caatcagaat ctgggaatga ccgacttctt
    20221 tatgaatcaa gatgcacaat taacaccggc tcaaaaaatc tttctccatt attttccttt
    20281 ggtggaaatc aaaaaaatac aggagatcta ttccaataag ttgttgaaac aagaagatga
    20341 tatttataaa aaacaaatcc tgctttttac attgatctgg atccagcgaa tcaggaaaca
    20401 tgaatttatc tctgccatta attggaaaaa tacagaaata aaagacagta gcttttctgc
    20461 atttattgac cacatgatcg aggaatttca attggacaat gtcccgcaag tagaaagaaa
    20521 ttatcttcgt ttcactattg agacgttcaa tgctagagat atctccaatt caatcgagtg
    20581 ggtacaagct caattactaa cgatccaatt gatccaattt gtagagaacg agacacacgt
    20641 acctttccat ctaaaggaag aagctttatt cgaaaatctc tataagcaca tggctgcact
    20701 gattgtaaga atcaagaatc atatccaagt catgaatcca ttgaaagaaa acatccgaac
    20761 aaattatttc ccaatctacc gcgcagtcac tcattttgtt cctacgatcg aagaagtaat
    20821 tggtggcaag atactggagg acgagatagc ttttcttgtc atccactttt cgacgatcgc
    20881 cagtgtaatc aagcgagact tgaattatgt ttataaatca gtcgttgtct gtaaccatgg
    20941 gatggctact ggaaatctgt tagcagagaa tctgaaagaa aagtttcctc agatcgagat
    21001 tactgcagtt ttaagttcaa aagaaatcga attagtagat aaattggatg tcaatctggt
    21061 tttcagcacc tttcaattga attatcgaaa taagccgctt ttagttgtcg atccgatttt
    21121 aactgagacg aatatcttgg ttatagaaga ctttttgaaa agaaatgaag aactacagcg
    21181 gttagagcct aacagtcatg aatcgacgga gttattcact caaataatag atgtcatgaa
    21241 agaaagtggt ggccgagtag atcgaatcat ctatggcaag ttagaaaaat tattcgaaac
    21301 aaacaattta gaaatcaaca aaagggagat ccagcctatg ttaaaagata ttttgacgga
    21361 cagcaatatc atgatcaaag aaaaagccac tacttgggag gaatcgattg aactagctgc
    21421 tagaccattg gttaaagaaa acattgtcga agagcgctat gtcgaagcga tgattcgtgc
    21481 agtgaatgat tacggaccgt atatcgtgat tggcaaacac atggcactag cccatgcccg
    21541 accagaagat ggggtcaata aattaggagt cagcgtagct acgattgagc aaccaatcga
    21601 tttcggtaat cctgaaatgg atccagtaaa gattattttt tgtttggcag cagtagactc
    21661 ctactcgcat ctgaacatca tgaaagaact aattgaattg atcaacaacg aagagaagtt
    21721 ggatcgattg attgaatgca caaatatcga agatttcaaa caactgcttt tttcagtagt
    21781 tgagaaataa taaatgatac gggggagaac aaagatgaac aaattgaaca ttttattcgt
    21841 atgcggtgca ggtttaggaa gtagttttgc agcacagatg tctgctgagg atgttttgaa
    21901 caaattagga gtagaagcca aactggacca ttgtgatatt tcttccgcag tttccatgaa
    21961 accagatgtg atcatcacag cagaaaattt tcgttcgcag ttcgaaaagt tcgcagttga
    22021 tcctaaaacg gcgatgatct atttgaaaaa tattgtttca aaaaatgaaa ttgaagaaaa
    22081 aatcacccca gttctgcaag ataagggagt tatctaaata caaaaagaaa agagggtaaa
    22141 aacatgggcg ttatcaattt tatcatcgaa aatattttga ctcaggcatc gatcaccatt
    22201 gcattgattg ctatgttggg tctactactg cagaagaaat cagcaggaca agtcatctct
    22261 ggcacgttaa agacactcct aggatttcaa gtgttgagtg ccggttcgag catcatcgtt
    22321 ggaagtttaa cttatttcgg gaaaatcttt actgaaggat ttcatatgca agggatcatc
    22381 ccttctattg aatctatcaa tggtcaagca atgaatgatt tgggtttagg tagagatatt
    22441 gcactgacgt tcttagccat ttttgtattc aatattattt tggctcgttt cacaaaatgg
    22501 aagtacattt ttttaactgg tcaagcaatt ttatggatgg caacgatgac gacagtcttt
    22561 ggctattttg ccggtctgcg agggatcgta ttgatcttgg tcggtggttt tatcggagct
    22621 tgtttcgcca ttgctatgcc ggcagtagcg caaccgatca ttcgtaaaat cactggatca
    22681 aatgatattg ctttaggtca tttttgtacg atcgggtact tatttgaagc cggtgttgct
    22741 aagctttttg gtgagaaagg cgaaaataaa aagtcgatcg aagatatcaa gttgccgaca
    22801 catttcgaat ttctgcaaga tacttatttg tctgtcatgg tcgtgatggt cccgctatat
    22861 atcatcactg ttttatttgc aggcgaacca ttcgcttctg aattatcagg agatcaaaac
    22921 tatatcatgt ttgccttttt acaagccatc caattcgtag tgggtgtgta tgtattgcta
    22981 gctggtgtac gtctactgtt aggtgaaatc gtgccggcat ttcgagggat agctatgaaa
    23041 ttagttcctg atgcgatccc tgccttagat tgtccagttt tctttcctta tagcccaaat
    23101 gctgtcattt taggtttcat tacgacaacg attggtacga ttattgccat gtttactttg
    23161 cccatgttcg gcttagcgat gattttacct ggtatgctaa ccaatttctt tgccggaggg
    23221 acagcaggga tatttggcaa cgcagttggc ggacgccgag gagcgatcat tggcggtatt
    23281 gctcatggtt tctttattac attattgccc gctttgctag taacgatctt taattcgatg
    23341 ggattcaaca atgctacagc aaccgatgta gatacagtag ccgcagcact cttatatgcc
    23401 tggatcttaa gcccagtttt gaaagcattt taattgaaag tagggaaaat gatgtttgga
    23461 ttttttaaga aaaaagaaaa agaaccgaaa aaagttctgg caaaagaaga aaaagcccta
    23521 tcgccagaag cgaccgaaaa gattcaatca gagattacac aattgaaaca agagatttcc
    23581 actacccaag ataagcataa attagcgaaa ctttatgaac aagttggact taaattttct
    23641 gaattgtatg tgaacgatca agccatccaa tatttagaaa agagtttgga aaataaacag
    23701 acgatcggag atgggtataa aaaactgatg agcctctata accagaagcg tgcagatgct
    23761 gctcgtgctg gtgatgatca agggatcgat tactacatgg gcaaaatgga tgagatgcga
    23821 caaattgcaa aacaagtaac gatcaaagga aacaaataaa aagataaggt ggcaataaaa
    23881 gatgtataca acattgaaag aagtaacaaa aacagcagaa gagttgaatt ttacagttgg
    23941 agcttttaat acgcataacc tagaaatgct tcctgctatg ctaggcgcag caaaagaaat
    24001 gggttcgccg atcattattc aaacaagtgt cgataccgct aaatatatcg gcatgcatgt
    24061 gttagtcaat gtaatgacag ctattgcaga agaagaaatg gttgatgcag tcttgcactt
    24121 agaccacgcc cgagactttg atgatatcaa agaagcaatc gacagcggat atacttctgt
    24181 catgtatgat gggtcccatc tgcctttcaa agaaaatatc ctgaagacaa aagcagtagt
    24241 agaatatgcc catgcgcacg gtgtttccgt agaaggagaa cttggaacaa tcggcggtac
    24301 agaagaagga atccatgtcg ctgaaaatga caaggtatat acaagaccgg aagacgcagt
    24361 gaaatttgta gaagctacag gcgtggatgc attagctatt gctatcggta cgaatcatgg
    24421 acaattcaaa tcaaaaacag aagtaaacat tcccttgctg aaagaaatcg atgcagtagt
    24481 tgatgtcccc ctagttatcc atggaggaac aggtgtgaaa gaagaagatt atcctgaatt
    24541 gatcaataac gggatccgca aattcaatgt tggaacggag cttttagtca actggacgaa
    24601 agtagctaaa gaaaccttta gtgaaacaga aatcaacaag tctcttcgcc ataatgtgat
    24661 tccagcaaat gaagcagtaa aagaaattgt caagcataag attggactgt ttatgaactt
    24721 ggaaagtcca attaacttag gaaataaatg agatgaaaaa gtatttcatt cttctggctg
    24781 gtagtcctgg tacaggaaaa acatatctta tgaataaact acgggaacga tttccagata
    24841 tgtatgccat cacgctcgat gaaatcaagg aatactatgc agaatccatc gggtttaaca
    24901 atttggaaga acgggcggaa caagaaagga cgaaagttta tcctttctat tacaaagcac
    24961 ttgatttgta tatggaagca ggaaagaaag tgattgtgtc cgagtatcct tttagtgata
    25021 agcaaaaagc acgactccaa gaattagcag atagatacgg atatcaggtg atcacgattc
    25081 gcctaacagc agactttgaa gtgttatggc aacgtcggta caaacgtgat cgtgaatcag
    25141 atcgtcattt gagctatatc atggatcatt atcactatgg tgacgtattg gaagatcgaa
    25201 cgaaagggac caatcatatt acgaaagaag cattcaaaaa gatcatcgcc gaccgaaaat
    25261 atgacaaatt ccagttagga acactatatg aatttgatgt aacagactat actcaagtgg
    25321 attacactcc actattggat aaattagaaa aagaaatcaa tgacaataag taacaaagaa
    25381 atggcttaat atcgaaaaat tggcttttga acgctgttta ccgagaggtc gtgagcctgt
    25441 cgttcagctc cgagtcacaa ggagcaattt caaaaaaaca gcttctcatg tttgatgaat
    25501 attgtgactt gtgaccgagg agttggtagg agaacaccgt ttagtgggga ggttgtgaaa
    25561 aagctgtcct gcctcaatta ataagaacag ccagacaaga atagctcgtc atatttttcg
    25621 tttggtgggg cttattaccg cagatgcaag cttttgaacg ccatttattc acagccccag
    25681 agaagtcgaa tgtcttttgt tctcgagttt tttaacattc ttttatttat tgcagatttc
    25741 attatattta tttgccgtaa ttaaaccagt cttctatact tgattacata gtcatacatt
    25801 tgctaaaaaa gaagggaaga gaatgaacaa gaagttggct caatacaaaa gactactcgc
    25861 actttctcaa gctggtctat attatggaaa agatgtattt gataaagagc gttttgagga
    25921 gatccggacg atttcattgg aactgatcag tgaaatagga aaagaaactt tcgaagaaat
    25981 aaaagcgctc acaacgattg gagaaggaca tccaacacca aaagtagatg ttcgtgccta
    26041 tatcaaaaaa gatggcaagg tcctactaat cgaagataaa cggacgaaag aatggtcact
    26101 tcctggtgga tttgctgaaa taggattgtc tcctgaagaa aatgtgcgta aagaagtcta
    26161 tgaagaaaca ggattaacag tagaaactac tcagttgcga gcagtttttg atacgaataa
    26221 acaaaaagat attccacagc tttttcagta ttataaactt gtttttgctt gtgcgatcca
    26281 cggtgaagag gccaagttta ttgagaataa tgaaacttcc aacatgggat tttttagtat
    26341 agaagagttg ccaaagctat cagaaaagcg aacaacgaag aaacagttac ttattttaga
    26401 aaacaaaaac cagatctatt gtgattg
//
LOCUS       DAYYKN010000027        26160 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000027 DAYYKN010000000
VERSION     DAYYKN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.027"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACHBB7_000952"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACHBB7_000952"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM9208919.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="ACHBB7_000953"
     CDS             1286..2461
                     /locus_tag="ACHBB7_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9208920.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="ACHBB7_000954"
     CDS             2465..3106
                     /locus_tag="ACHBB7_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9208921.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="ACHBB7_000955"
     CDS             3103..4020
                     /locus_tag="ACHBB7_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9208922.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="ACHBB7_000956"
     CDS             4017..4688
                     /locus_tag="ACHBB7_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9208923.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="ACHBB7_000957"
     CDS             complement(5229..5717)
                     /locus_tag="ACHBB7_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM9208924.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="ACHBB7_000958"
     CDS             complement(5918..6184)
                     /locus_tag="ACHBB7_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9208925.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="ACHBB7_000959"
     CDS             complement(6184..6447)
                     /locus_tag="ACHBB7_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9208926.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6968..7108
                     /locus_tag="ACHBB7_000960"
                     /pseudo
     CDS             6968..7108
                     /locus_tag="ACHBB7_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(7250..9073)
                     /locus_tag="ACHBB7_000961"
     CDS             complement(7250..9073)
                     /locus_tag="ACHBB7_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9208927.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="ACHBB7_000962"
     CDS             complement(9671..12376)
                     /locus_tag="ACHBB7_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM9208928.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="ACHBB7_000963"
     CDS             complement(12682..13629)
                     /locus_tag="ACHBB7_000963"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM9208929.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="ACHBB7_000964"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="ACHBB7_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM9208930.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="ACHBB7_000965"
     CDS             complement(14264..14626)
                     /locus_tag="ACHBB7_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9208931.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACHBB7_000966"
     CDS             complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACHBB7_000966"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM9208932.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACHBB7_000967"
     CDS             complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACHBB7_000967"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM9208933.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="ACHBB7_000968"
     CDS             complement(16254..17765)
                     /locus_tag="ACHBB7_000968"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9208934.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACHBB7_000969"
     CDS             complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACHBB7_000969"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM9208935.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="ACHBB7_000970"
     CDS             complement(19592..20668)
                     /locus_tag="ACHBB7_000970"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM9208936.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="ACHBB7_000971"
     CDS             20864..22609
                     /locus_tag="ACHBB7_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM9208937.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22889..23326)
                     /locus_tag="ACHBB7_000972"
     CDS             complement(22889..23326)
                     /locus_tag="ACHBB7_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM9208938.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23310..24683)
                     /locus_tag="ACHBB7_000973"
     CDS             complement(23310..24683)
                     /locus_tag="ACHBB7_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208939.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25005..25493)
                     /locus_tag="ACHBB7_000974"
     CDS             complement(25005..25493)
                     /locus_tag="ACHBB7_000974"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9208940.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25515..26117)
                     /locus_tag="ACHBB7_000975"
     CDS             complement(25515..26117)
                     /locus_tag="ACHBB7_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9208941.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7504 a         5561 c         4837 g         8258 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat caggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc
    13741 gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 ttgatagcat caatcaattt attcttttct ttttccaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc
    19801 cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt
    21241 agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg
    21961 tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca
    22801 tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt
    22861 acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa
    22921 aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc
    22981 ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt
    23041 cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat
    23101 aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac
    23161 tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat
    23221 aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct aattttatga
    23281 catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc
    23341 ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt
    23401 gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat
    23461 aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa
    23521 aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc
    23581 attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg
    23641 tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat
    23701 aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa
    23761 atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa
    23821 atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg
    23881 aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat
    23941 tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa
    24001 atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat
    24061 aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg
    24121 tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa
    24181 aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct
    24241 tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc catattgata
    24301 aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca
    24361 ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt
    24421 ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct
    24481 agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc
    24541 gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa
    24601 ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca
    24661 aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa
    24781 aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag
    24841 cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa
    24901 gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc
    24961 ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt
    25021 ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta
    25081 atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc
    25141 aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa
    25201 gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt
    25261 ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag
    25321 taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc
    25381 atgtaatcca cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt
    25441 atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca
    25501 cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg
    25561 ctatacacac ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa
    25621 gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc
    25681 aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat
    25741 ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca
    25801 agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat
    25861 ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg
    25921 atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg
    25981 ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc
    26041 aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact
    26101 gatccttttt tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
//
LOCUS       DAYYKN010000028        25358 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000028 DAYYKN010000000
VERSION     DAYYKN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.028"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACHBB7_000976"
     CDS             complement(132..509)
                     /locus_tag="ACHBB7_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208942.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACHBB7_000977"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACHBB7_000977"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM9208943.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACHBB7_000978"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACHBB7_000978"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM9208944.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACHBB7_000979"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACHBB7_000979"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM9208945.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACHBB7_000980"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACHBB7_000980"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM9208946.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACHBB7_000981"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACHBB7_000981"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM9208947.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACHBB7_000982"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACHBB7_000982"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM9208948.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACHBB7_000983"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACHBB7_000983"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM9208949.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACHBB7_000984"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACHBB7_000984"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM9208950.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACHBB7_000985"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACHBB7_000985"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM9208951.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACHBB7_000986"
     CDS             10661..11575
                     /locus_tag="ACHBB7_000986"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM9208952.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACHBB7_000987"
     CDS             complement(11708..13273)
                     /locus_tag="ACHBB7_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9208953.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACHBB7_000988"
     CDS             complement(13266..14717)
                     /locus_tag="ACHBB7_000988"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9208954.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACHBB7_000989"
     CDS             complement(14731..15747)
                     /locus_tag="ACHBB7_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9208955.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACHBB7_000990"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACHBB7_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9208956.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHBB7_000991"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHBB7_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9208957.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHBB7_000992"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHBB7_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM9208958.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACHBB7_000993"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACHBB7_000993"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM9208959.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACHBB7_000994"
     CDS             21340..22764
                     /locus_tag="ACHBB7_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM9208960.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACHBB7_000995"
     CDS             complement(22806..23063)
                     /locus_tag="ACHBB7_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208961.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHBB7_000996"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHBB7_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM9208962.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACHBB7_000997"
     CDS             24337..25314
                     /locus_tag="ACHBB7_000997"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM9208963.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8202 a         4317 c         5695 g         7144 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DAYYKN010000029        25123 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000029 DAYYKN010000000
VERSION     DAYYKN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.029"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACHBB7_000998"
     CDS             6..245
                     /locus_tag="ACHBB7_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208964.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACHBB7_000999"
     CDS             312..521
                     /locus_tag="ACHBB7_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208965.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACHBB7_001000"
     CDS             581..1252
                     /locus_tag="ACHBB7_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM9208966.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVLAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACHBB7_001001"
     CDS             1305..3281
                     /locus_tag="ACHBB7_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9208967.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDITQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3296..4048
                     /locus_tag="ACHBB7_001002"
     CDS             3296..4048
                     /locus_tag="ACHBB7_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9208968.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4064..4819
                     /locus_tag="ACHBB7_001003"
     CDS             4064..4819
                     /locus_tag="ACHBB7_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM9208969.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            4832..5092
                     /locus_tag="ACHBB7_001004"
     CDS             4832..5092
                     /locus_tag="ACHBB7_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208970.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5089..7179
                     /locus_tag="ACHBB7_001005"
     CDS             5089..7179
                     /locus_tag="ACHBB7_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFM9208971.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7206..7670
                     /locus_tag="ACHBB7_001006"
     CDS             7206..7670
                     /locus_tag="ACHBB7_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208972.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            7667..7909
                     /locus_tag="ACHBB7_001007"
     CDS             7667..7909
                     /locus_tag="ACHBB7_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFM9208973.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            7927..8229
                     /locus_tag="ACHBB7_001008"
     CDS             7927..8229
                     /locus_tag="ACHBB7_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208974.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8299..10362
                     /locus_tag="ACHBB7_001009"
     CDS             8299..10362
                     /locus_tag="ACHBB7_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208975.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10362..12974
                     /locus_tag="ACHBB7_001010"
     CDS             10362..12974
                     /locus_tag="ACHBB7_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM9208976.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            12971..15502
                     /locus_tag="ACHBB7_001011"
     CDS             12971..15502
                     /locus_tag="ACHBB7_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM9208977.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15552..15884
                     /locus_tag="ACHBB7_001012"
     CDS             15552..15884
                     /locus_tag="ACHBB7_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208978.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            15885..16478
                     /locus_tag="ACHBB7_001013"
     CDS             15885..16478
                     /locus_tag="ACHBB7_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208979.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            16641..18665
                     /locus_tag="ACHBB7_001014"
     CDS             16641..18665
                     /locus_tag="ACHBB7_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFM9208980.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            18665..19864
                     /locus_tag="ACHBB7_001015"
     CDS             18665..19864
                     /locus_tag="ACHBB7_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9208981.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            19880..20524
                     /locus_tag="ACHBB7_001016"
     CDS             19880..20524
                     /locus_tag="ACHBB7_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208982.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            20535..21341
                     /locus_tag="ACHBB7_001017"
     CDS             20535..21341
                     /locus_tag="ACHBB7_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208983.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            21363..21593
                     /locus_tag="ACHBB7_001018"
     CDS             21363..21593
                     /locus_tag="ACHBB7_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208984.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            22107..22415
                     /locus_tag="ACHBB7_001019"
     CDS             22107..22415
                     /locus_tag="ACHBB7_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208985.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            22417..22839
                     /locus_tag="ACHBB7_001020"
     CDS             22417..22839
                     /locus_tag="ACHBB7_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208986.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            22859..24448
                     /gene="mobP2"
                     /locus_tag="ACHBB7_001021"
     CDS             22859..24448
                     /gene="mobP2"
                     /locus_tag="ACHBB7_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFM9208987.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            24460..24795
                     /locus_tag="ACHBB7_001022"
     CDS             24460..24795
                     /locus_tag="ACHBB7_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208988.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            24936..>25123
                     /locus_tag="ACHBB7_001023"
     CDS             24936..>25123
                     /locus_tag="ACHBB7_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208989.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         9386 a         3904 c         5155 g         6678 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtacttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcagaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgagtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ctatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttattggaa tgttaggaat cattgcaggc tgctttcagg
     1381 gatatcgagc atatgcagaa gatatcacgc aaaaaacgcc accagaaaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgactgaag
     1621 attctgatct aaccaacgcg aaagtggacg aaatcgttaa agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacaa acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagattac ttttgctgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gactgataat acaggttcag gttttttaaa agacattcac ttatatccta
     1921 aaaatattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcacagctc
     1981 aaagcaaaca aacaccttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attaggagat cttgtatcgg atgatcaagg aaagctgact gctactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcaga agttgtagtg ggatcagata
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacaattca tatcccaaca gacattgcag
     2401 gcggagcaga cggtatcaca gtaaacggag tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg ggatacagca ggacaaggac tttcttatgt cgctgctaaa gcaaatatca
     2521 aagtaacaaa taaagatgga tctgtggtat taaaagaaaa tacagattat aaaatccaaa
     2581 attcagaaaa tggctttgtg atcgatttta tcgtgaataa tggccaagta agtgatactg
     2641 tagcaagttt acacggacaa gatcttcaaa tgacttataa catgtatgta aatgacagcg
     2701 cagctgttgc caatccatta acaaatagcg tggatttcgt ttataacaac aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggt gcaaaattct
     2821 taaaagtcga tagcggacta tttggaacag gaatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgctgc taaaaacgca gaaggaaaat attatggtgg attagttgat acggataaag
     2941 acggtgtcaa agaagcagta tgggtagacg atgtagcaaa tgcagcgatc ctaaaatcag
     3001 ataaagaagg tcactttgaa atcactggtt taactgaagg tgaatattca ctggaagaaa
     3061 caaaagcgcc tgaaaactat caaaaattaa cgaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaacaa tcaaaaagct tccgtaccaa
     3181 tgaccggtag caacggtttt caaacgtatg tattaatctc ttgcttgttg ctaggagcag
     3241 gggcactttc tgcagttgtt tactttaaga agaaagccta gaaggatcaa aaccaatgat
     3301 tatcaggaag cctaagaaaa agcgaatcat gggtaaatgg atcattgctt tctggttact
     3361 ttcagctgta ggtgtcttac ttttgatgcc tgctgaagcg agcgtggcga aataccaaca
     3421 aaatcagcag attgccgcaa tcgatcgaac aggaacggca gcagaaacgg attcttcttt
     3481 ggatgttgct aagattgagt taggcgatcc tgttggtatt cttacaattc taagtatttc
     3541 tttaaaacta ccaatttatg acggaacgtc cgacaaaatt ttagaaaatg gtgtaggtat
     3601 tacggaagga actggtgata ttacaggagg aaacggtaaa aatcctttaa tcgcaggtca
     3661 tagtggactt tataaagata atctttttga tgatttacct tcagtaaaaa agggagaaaa
     3721 gttttatatc aaagttgatg gtgagcagca cgcttatcaa atcgatcgga ttgaggaagt
     3781 gcaaaaagac gaactacaaa gaaactttgt cacttactta gaatcaaatc ccaatgaaga
     3841 tcgagtaacg ctaatgacat gtacgcctaa ggggatcaac acacaccgtt ttctagtgta
     3901 tgggaagcga gtaaccttta caaaatcaga attaaaagat gaagaaaata agaaacaaaa
     3961 attatcttgg aaatggcttt taggaagtac tgtttttctt tcagtcatga tcattggttc
     4021 gctatttgtt tataaaaaga aaaaataaag gagggtaacc aacatgagaa atagaaaatg
     4081 gctatttgtt tgtgcagccg ctcttttttc tttcactgcc tacacacagt tgacgtttgc
     4141 agacagacaa gaaagcacgg atagcacaga acaaacaatc caaacgtcag atagcagcac
     4201 gattcaaagc agcgatgaaa ccgtcgattc gtcaacagtt gattcttcag aagaacctaa
     4261 aattgctgaa gacgatcaaa tagtcaaaca gaccattcac gttcaaaaga ttatttcttc
     4321 agaacaatta gacaaccagg ggcatgaaaa acaagaagga gtaaatggcg cgaagttttc
     4381 tgtttatgat gtatcggaca ttttacaaaa gatggatgtt aaggatctca ctactgacca
     4441 aattgaatcc caattaaaaa atcgagtgaa aaaactatcc agtgatcaat tgaagctagt
     4501 ttctaatgaa gaaacaaaga caattgatca acaaacaggt gtctttgaat tctcggtaga
     4561 agtacaggca aatcagaaac aagcttatta tattgtcaat gaatcaagtc ctgaaaacat
     4621 ttctaatagc gaggatattt tgctattaac acctgtttct gataaaaacg gtgaattctt
     4681 aaaggatgtg tggatctatc ctaaatcaga agcttcgcaa cctaaagaag aagtaaaaaa
     4741 aatcgtttct actggcgtaa agaaaaactt ctttgagaat tgttgggatt ttgttactca
     4801 cttgttttct agagattaag gaagaaataa aatgaaactg atcaaattat tattattcaa
     4861 tttttcttgt ttaaccgcag tctttttgta ctgcggttta ttattttcta aactatctat
     4921 tttgccaagc taccaactat acctaatgag gattttaaac ataccagctg cttccatgcc
     4981 gctagatatt atggaaaagc tcattaggaa tccattattt actacgatca atttgatcgt
     5041 tattgtcgga atttcactag tcatttataa aaaagagggg atgaagggat gaattttaaa
     5101 cactcgtgga caagacggtt aaaatatgtt tttcccataa tgatgatgtt aggtacattg
     5161 tttgggttaa aaacagccaa tgtctttgct gaaaaagtta ttgtcgatcc gtcaaatcca
     5221 atccagaacg taaagaataa ttttattatt ccaaaagatg ttcctaatgc aaaaacaaat
     5281 attactgtaa acggagcgtc aaaagtttac aaatatcatg ttgatgccaa caaaggtggt
     5341 tttacagacg actacgtagg attagtggag ggaaacaaaa atatacgcta tccttctttc
     5401 caaaaagaat acggtgaaac aaaccttgtt ttagaaggcg taacaaccaa caccaaagtc
     5461 aatattgact atggaaaaat cggtacgtat aacgggaaaa aagtcaatat caagctagtt
     5521 ctatctaata ttcacttata ctcggatacg ttgccgtgga atctactaga taacaactat
     5581 acaaaaactc attttcgaga cgatggctat aaaaatacga atggtgcaat gagtaaatct
     5641 aaaaaaagaa cagtactttg gatctcagac aatcttttct caggaattgt ttaccattca
     5701 acacaaatga atgtgcagct agtagccaca tatgaggacg gtagccccgt tcaattttca
     5761 ggagatacat ttatcagttt taactctttg aaccctgcag gaggaaaaag cacggatctt
     5821 aaaggagaat atgcccacta tgacaaaatg aatacgacag attggtatgt cagaaaagat
     5881 acggttcttt cagaatttaa atctttctac aacaatctga acgtggtagg tggccaccca
     5941 ggaggaagct ctaagctgac acaagcggat aatgatttca ataatttgca tgataaatta
     6001 ggagatccga agtttggtca aggtactgtg tcctttaaaa tatctgaagc aaatccgacg
     6061 tttgtgattg gttcttctaa tgttcaaacc tggtttactt tatcttctgc gacaatcttt
     6121 agtgttgttc ctgatcaacc tgaaaaaaca ggggttgata aaaatggaaa taacgtgaac
     6181 gataaaatgt tgcaagtagg agacacgatt caatatcgaa tcaaacagaa ggtgaatcga
     6241 ttaggagtgg atttactagc ggtttacgat cgatttgagt taatcgataa tttaccaaaa
     6301 gaagtaaatt acgtcgatgc acacgttgaa agtggtacaa ataaaaaatt cgatgtatct
     6361 ggtgaagtga cctatgacaa aacaaaacac caggtaaaat atgcagcgaa agctgataca
     6421 ttaaaaaatc gaatgaagta taacggcgaa acttacgaat tagtgatcaa tgtaaaagtc
     6481 aatgaattgg ccaatcaaaa tagtgtagcc aaaaatcagg gaacgtcgat cattaataaa
     6541 gtagaaaagg atacaaatat cattaccgtt tatttcccta aaattccagt gaaagaagta
     6601 caacaaaatg gaaaagatgt aaacggacga aatgacggta agaagggtac gccgacagct
     6661 cctttaaacg caggatctga agtacaatat ctagtcacac aaaaatggca cacaaaaggc
     6721 gtagacgctg cgtcagatca ctacaaacaa tttagtattc aagatccaat tgaagctcgt
     6781 ttgacatata aagaaggttc tgcgcaagta atagataaga gtaaaggaaa agatattact
     6841 tctgaaggca cactaaccta tgatagcaat tcaagaacgc tgaagtggga agcttccgct
     6901 gacttcttaa gtaacaatct tttagatgga cgagaaattc agctgatatt tacagctaaa
     6961 actccattac aatcagaaaa aaatattgat aaccaagccg tagtagcagt agataatgtt
     7021 tctaataaaa caaatgttgt gacaattggg gttgatccta acttgccaca ggtcattgtt
     7081 cctaaaacag gttctacaca tttagtaacg atttcagcgg tcagcttact attacttgtg
     7141 ttagcaactt ttagttatgc ggttctgaga tacatttaaa ataaaaaaac gaaagaatga
     7201 ataaaatgaa aaagttaata atagtagtta cttccttgat tgccatgatt gttattttag
     7261 tcggatgtag taatgaaaaa aataaacaaa ctaatcaaga taatggcgta ttgaaatcag
     7321 gaactatgtg gaaagaagaa gtaggtggtc ttgtttataa tttgaaaatt atagacgaga
     7381 ctacctggga atactctgag agtgtatggc atccagatcc tgtacaaata acagtcaaga
     7441 ggcaaaaaga ctataaaggt ttagaaagat acaaaatagt agattcagct ggtgttaggg
     7501 aatttataaa caaatctgat tctttattta ttgttgttcc ctatgagaag aatggcgtaa
     7561 aaaaaataat ttttcttgga tctagcaagg atgaaaagcg aacgaaagag aaattgattc
     7621 acgatggatc acaatccaat aaatataagt tacaaaagac tagcgagtga ttgctagtct
     7681 ttttatttgt cctgaaggag gaatcacagt ggcaaaagct aaaacgccat ttgaaaaagc
     7741 cgcgtctaaa atcttagaac gccgcggtgt atctgaaaca cagtggaaac aagaagtcct
     7801 tgaaaaagca caatttgacc tattacgggg aaaagacaag gacttagaag gatatatcta
     7861 tcaacgtgaa cgtgaaaagc ttgttttcga tgaaattatt aaacactaaa aggagcgaac
     7921 aattttatgt ttgaatcaat tacaatgaat tttttaacac aaccgaaagt agtagctgcg
     7981 gttgatattt ttagagcatt acaaactgcc gcaactagtt taaaaaacaa tctgatgatt
     8041 tttgctgctg ctgcggccgt tgcttccatt gttattgggg gtcttttctt cctatttgga
     8101 cgaagaggag ctgaaaatgg gaagatgcac gtaggctatg tattgatggg ggtaggagtt
     8161 attttagcag cagcttctat tgtggtttcg ttatttggat tgtttggaca aacacctcaa
     8221 ggattataag aaagtaaaaa agtaggagta agaagtagtc gatacttttt actccttttt
     8281 tatgaaagga gaatactcat gtactttacg attgcaggca ataccaaaac agataaagag
     8341 ttaataaaaa aagcaacgaa ggaagtagga aagcgtactt cttttttacc gaaaacaatt
     8401 aaatattctg tcattgaaac ggaagaacag ctacatgtgt tagggaaagt actggaacac
     8461 gaaagtcgat cttctgttaa gttgactctc acgttacatg acacagaagg aaaaatagag
     8521 aaagctggta cagaatacgt ttttggccca ctggattatc aaatttcaga aggtttctat
     8581 cctacattga tggatttaat ccgccttgat gtgtttaaag aaacggatga aactatgagt
     8641 gtggaagagt cttatcgaaa cgaaaaactt ttacaagttt tagaagaaga gttagagagc
     8701 gaccaaagaa aagtgaatca agggagtgaa gtcgaagtaa atgaagaagt accatgggaa
     8761 cgaccaatat ttccaaagaa ggagtcaaaa gaaaaagaga cagtctatgt tgacgaacaa
     8821 gctgatattc cgactcagaa atcgatattt gaggaagaga acgatgtgat gggtcaattg
     8881 gaggtagatc cctttgaaga aacaatggag gatgaagaga cggccgaagt agaagttgat
     8941 ccagttgaag cgaatgaaga gtcaacatgg actgaagggg attctcagga atatattgaa
     9001 ccacttatca gtgaagaagt agaaaaaaac gaaatagaga tacaaaaggc aactgtattt
     9061 gattatattt tggatcaata tccgccagag catgaatgga tgaaagaaaa aaacaaatta
     9121 tttttaaatg agctttattc gaaatatcaa ttaccccaaa cctatcaagc atttttagag
     9181 agtaaagaag aattaatctc acagggtaaa ggcacacttt tagaacagtt agagtctgtc
     9241 caatcaacaa attggcatga gcttgcacaa gaagatttat tagccacctt tgaagaacgt
     9301 caacaagaaa cagatcaaga aattaatgag tatgcgacgg aacagtcgtc taattgggag
     9361 aaagcaaaag aggaactaaa taaagaagaa cagcgagtga ttgaagaaga agtacaaaaa
     9421 attaaagagc gatacgaaaa gaaacgcgac aatgcgtatt ccttgtgtgt agcaaagatc
     9481 aataatttta ctcagacgaa tcaagaagcg ttatcgaaag aaaaagaaaa cttgttaaaa
     9541 gaacgtatta aagaacgaaa agaagaattt taccgaggtt tgcgtacaga taagctaaag
     9601 attagcaaag aattaaatga acaactgaat aagctatatc aaacaacgac tgaagttctt
     9661 atggaaaaat acgaaaagat ccagcaagaa ttacaagtgc aaattccaaa atggaaaaaa
     9721 gagtatgaaa ttcaacaaca agaattgaaa gaacaagaag aaaaacaacg gcaaaaagaa
     9781 aaagagcgcg aacaattaga actaaaaaaa cgtgaacagg atcttgcgga acaacaaatg
     9841 gcaatagaaa aacaacggtt aaaccaagaa aaggaaaaag aagcagctgc tcgtaaggaa
     9901 cgtcaagaac aaatggatac attgcgacaa gctcagttaa attatctagc acaacctcaa
     9961 ccaacgcctt cagtagctgc tgttcaaacc ccaatgaaca accaaacaaa tctaactcct
    10021 tcaccacagc gttcctttaa cggatggatg gtgggatgta ttgcaagttt aagcttgctt
    10081 ctaggtggag gaacagtatt tgcttttaat catatcaatc aatcttcaca ggctgattct
    10141 gtggccacaa cgacgtcaat caaggaagaa gcccaagaac aagcaagaaa agagtatgaa
    10201 caacaattag aagaattatt gaatcgtacc aatcagtcaa caacagagtc aagcatggaa
    10261 aatagcaaag agagtgattc tagtaaggct aaagaaggat caagcgattc aactgtacaa
    10321 tcaaacactt ccgaaagttc agaatcaaat gaggtgaaat aatgcgtgtt agagaaacaa
    10381 cgaagcgaaa agacaaacga ttcaatttag aaaagaatag gtatctttct catttctatc
    10441 gctatactcc ctcaggagaa gagctaccag aaaaacatga caaacgcatt tcgaaaaaga
    10501 ttttttatag tggacttgta tacctccctt tagagtattt agtaacaaga gtaccagaag
    10561 ttttcaaccg attggaaatt gttaagggaa gcttacaagg aataaatctg tttcaattac
    10621 aggaaatgcc acaggtcttt cattttgatt cagctggttt ttggaatcca tttactccta
    10681 caactagttt gggtacacgt ttagcaattt cgacagtttg tgtggttcca gctgtactta
    10741 tgggattgaa aatggacatg tggtttcgtc ctctagcaga tggacaaaaa ggggataatc
    10801 gattaatgac cttaaaagaa atccaacaga tttatccgca aatacctgaa acaaaaacta
    10861 cttttccagg ttatggaggt attccaatca cacattacaa aaaatattgg tacattcaaa
    10921 cagatactgt taatacgtgt attgttggta cctctcgatc agggaaaggt cagattgaag
    10981 tgttagccac cattgacaat ttatctcgtg cagaaaaaca aagttcaatg gtcgttaatg
    11041 atccaaaaac agaattatat gttggtagta aagatgaact agaagctaga gggtatgatg
    11101 tatatgcctt taatatcacc gatccactgc aatcaatgag tgataatcca ttggcactca
    11161 tcgtgaaata ctggaaacga ggagacatag atactgcaac acagttaacc aatactttca
    11221 caaatacgat ttattatgat gccaacgctt cagataacaa atactttaac gaaaacgcgc
    11281 agaaagctgt caatgcgctt atttttgcgt tgttagaaca agctgatcaa acaggagatt
    11341 actccaaatt gacaccgaat aatctcgttg agcttctttc tgagattggc ggttttaatt
    11401 attatccaga tccagacaat gaattcaaac agttgaatgc cttagatgaa tttatgaatc
    11461 agttgccacc tggaaacatt gcaaaaaaac aatatggggc aacgaaaatt ggatctgata
    11521 aagcgaaggg aaatatttta tcaacggcaa tcacgggatt gaatccattt actctaacga
    11581 aaattgcaaa gatgacttct caatcaacga ttgattataa atcggttggg tttccaaaat
    11641 acttagattt gaaattacat gaaagtcttt taaatcaacg agtcgtagta gaattttatc
    11701 ataagggaaa gaaatttcat gaagaacgtg tcaaagtagg ctataaagga ttttgtgaaa
    11761 tcaattttga ttgtcattta gttgcaggtg atcaagtttg tttaaaatac gatcatcaag
    11821 gaaaagaata tgtggcttgg tatgaatttt ctcaaagagt attgaaagac gaagagggta
    11881 atatcgttta tcggaaaaaa cgaggagaaa cccatttgcc agaattagaa aaagaagccg
    11941 ttctaacatt agatgaggag aaaagtaact tattagtaga gtctattcgg atgcactata
    12001 ccgataaacc aatggctatt tttatgctta cacctgatta tgatccatct aaccatatca
    12061 ttgttagtat ttttcttagt cagttatata aagaattatc tacgcaatgt gttaagacta
    12121 aaggagataa atgtcaccgc aggattcatt ttattttaga tgaatttggg aatatgccac
    12181 caatggataa tatggaagga atcatgacgg tcacagctgg ccgaaacatg ttgtgggatc
    12241 tgtttattca atcttatcag caactttatg cgaagtatgg agaacaaaac gcagcgatta
    12301 tcaaagaaaa ttgtcagaca catatttata ttatgtcgac agatgataat accatcgaag
    12361 aattttctaa aaaagtgggg aataagacgg ttgaacaaga gaacagtacc atcaattcat
    12421 taggtatgaa tactcatatc aatcgcaatg ttgattcaga tcgtatctta actcctgaaa
    12481 gaatttctac ttttttagaa ggtgaaacga ttgtactcgc tcctttaaaa cgacaagatt
    12541 tgaaaggtca taaaatacgg ccgtatccta tttttaatca tggggaaacg aatcttcctt
    12601 atgcttatca atatattgaa gatttcaatc catcgtggga tatcaatgat atagacatac
    12661 attgtacaca tgcggacttg gatcttaagg ctttacaaat tgattatgat tatttcctac
    12721 cagatgaata tgctagaaat ctctatcatg gactgcatga tcaattagaa gaaaaaaacg
    12781 aagaaaatat gttttataaa atcgttcaat ctcttgagaa tgagacggct aaaaaattgt
    12841 taattcaatt ttacgaagaa caagcagcag atcgacttca agaatatgtt gaaaagttga
    12901 tcaaaaataa agaaatttct gaaatgattg ggaaacggtt gttattggaa tctgaaaagg
    12961 tggtgaaaga atgatcattg gaggaatttt ttctggcata gccgactggt ggtcaggctt
    13021 attcggagaa ggggatgcaa accccaaaac cgtaacgaaa gtcttacaag attatggtaa
    13081 ttatctgcag tatcaagatg tatttagttg gatttggaac acttttctgt ggggattgat
    13141 taaaggatta tataatctca atactttact agaaaaaacc atttatcaaa gctttgattt
    13201 aaaagactta ttgaacgccg ctggggtaaa tgcactattt caaagtttga ttagtaaggt
    13261 attggctctt ttttgtgtcg tcacattaat ttatttaggc ttgaaatttt ttatgagtaa
    13321 acacccacca aaagtcaaaa atatcttggt taatttattt atggccatga tcttgatttt
    13381 tggtgggagt tctttaatcg atgaagggtt aaatatcagt aaaagctttt acggagacgt
    13441 cactgcagca aataaagcag ataaaacggg ttcacctgct tttcaaatta ttcaaaataa
    13501 tgtgatcgat gtcagtacat tactagcaac tgacccgaat aaagtagaat ccctgccaac
    13561 agacaaaaga aatgctttaa cagcgaaaaa ttttaaaaca gcgaatatca atgggattat
    13621 tactcctaaa caagctaaag aaatggcgga taaagataag agtggtttat cagatttaca
    13681 aaaggatcgg ttaagagcgt tacaataccg attggaacta gatgataatg ggaaagaaat
    13741 tcctgttcaa attagtgacg aaggcttagc aaaatatgtc tatacttcag ggtatcgtcg
    13801 atacagtagt aaaccaggta ttatcttagc tggtgaatct tctttggcgg tggcctattt
    13861 atttatccta tttacgatcg ttacttgcat tattgagctg gtatttaaaa aattctattt
    13921 agtgattgcc gcgtcaacgg actttgagac aggccaacgg atgaaaagtg cgatagaaga
    13981 tatttctcaa tccttcttgt tattggcgtt tacgatttta gaattacgaa tctatacatt
    14041 aatgctttca ggtatcggtg atttgcatgc ggcaggaaag atcaatggat tcttgtatgt
    14101 tgttgcttta attgtgctaa ctattgcgct attcaaaggc tctcaatcgg ttacgaagat
    14161 tttcggtgtt gatacgagtc tgaaaaacgg cagtaattct cttcttagca tgtttgcctt
    14221 aggaaatatt gcgaaaaatg tgggtagcgg agctaaaaac cttgcaagtg ctggtaagag
    14281 tggtttaagc catttaaatg atattcgtaa aaatggattt ggccatcgaa aagatgttac
    14341 ccctgataac ggggaagaac aatcagacga taaagctaca actgtaccta atagtaagaa
    14401 aacagtttcc gaaatgttcg ggaaagcgaa aaatggtgtt tctaaagccg cagaaggtct
    14461 tggttacgtc aatgaacgtg ggttaaaagg aacagctgaa gatcttacag aaaaagccaa
    14521 agacacctta gaagagaaat tagacggcac gagagctaaa gaattggctg aagctgtgaa
    14581 gaatccatcc gaagcactag aaaaacaaaa agaaaaagct tcagaagtca aagaaggact
    14641 aaaagatgcg tatggtgaag gacaagttaa tgcagcagta aaagctaata caacaaccga
    14701 aaaagaaaat caagaaggta tagtgattcc ttcagtcaaa gatcaccaag aagcgttgca
    14761 ctctaatgaa aattcgattg aagagaaaaa ttccatagaa gaaagaaatt cagaagaagg
    14821 aaaaaatgga cagttaccaa aaactgatcc agaacaatat cgctataaac ctaacagtga
    14881 ccttgaaaat gagaagcgaa tttcagattt atcaaaagta aatcaagaag acaaagcaga
    14941 aaatgaggga cagttaccaa aaactgatcc agaacaatat cggtatagat ctgttaactc
    15001 cgaaaatgaa aaacgcattt cagatttacc aaaacgaaat caagaagtac aaacggatga
    15061 ggattccatc gttctgccaa atgataatgg aatcgaagtt ggaactgaaa aatcaacgaa
    15121 aattggttta cctaaggcac aaaatttacc aatgaaagat agtcgtttga atcaattcaa
    15181 ggagaatgat caaagctttg ttcaccaatc aactgcaggt actgttcaaa tgaaaccaag
    15241 tcgtttgcct gaaaatggaa atctgacaac tgtttcttac aatttatctg aaagtggtga
    15301 gacagggaat gcgtccgttc aaaatgttgt gacaagagaa gctgcaccaa aacctattca
    15361 aaacgttcat acaggttaca agccagcaaa tgtggcattt actgcaccaa caaaaataaa
    15421 tgtgacaaga gaaactaaaa acaggaacaa tgcattgaaa gaacaactat tgagggatat
    15481 caaacaggat atcaaaccat aaaaggccta caagttaggc cttttattta tatgaaaagg
    15541 agaaatcaat tatggataaa tatataaaca tgaaaaaccc tccaagattt agtggagaat
    15601 ggaagttttt cgcttttttc tttttagatc tattaacgct tggtggctgc tttttttggg
    15661 cgaaaatggt caataatctt gtccacttac cactcttttt tgcactcttg aattatggct
    15721 tatggattgc gatagcgtta gtgattgttt ggcgacccta taataaccca ggtcaacgac
    15781 aaatttttgc gattatcgag tctatttttt ttgcggacaa gaaccattat catgcgttag
    15841 atcctaaccg aaattatcat ggtacgtata aggaggatga ataaatgcga gtcaaaggac
    15901 aaaattcagt tgaaaggaaa tccattttgc agtttggaaa accagaagag cgctctatct
    15961 tggacttatt tccgtataaa tcctgtacag aagagggagt cttaattact agagaagatc
    16021 attttcaacg ttattatcgt gtagtttcta cagacgtgga aggattaaat gaacaagaga
    16081 aactagaacg aatgaatcag ttgaccaccg tcatgcgtac gtatgtgcca acaattaagt
    16141 tagttagttt gaccacagag acggatctaa cggaacaaat cgtgcaaaag cggcaattat
    16201 tagataaaaa tcgtatggaa cagtccttag gaagaaatct acaacgatta aaaaaatatg
    16261 aaaagaaatt agtcgaagaa atccaggaat tgaaacgagc agaacaagaa cgtcctgact
    16321 taagcttctt tttcgtcatt gaaggaaaga caatcaagga tttacaaacc agaaaccgtc
    16381 agttaatgcg atctagtggc attttagggt taaaaccact ctacaaaaaa gaattgatca
    16441 agattctgta tcgaatgaac aatatgaatg atgagtagga atggagaaga gagaatgaaa
    16501 tatccgttga tatggttaga aaaagatgtt ccgaaggaag agaccactag aaaatttcct
    16561 cacactttaa gcctgggtaa gttgggaaaa gggcaatctt ttaaccaagt aaagaatcaa
    16621 acaagaagag gtgactgtac atgttagaag caattaaaac agctttttca ccacaaaatg
    16681 atcaaaaagt acttgatcca ttaaagcaac taggaaatcg ggaaatagaa attttaaaat
    16741 ccaaagggta tgatttagat tttttaaaac aagtgatgcc acaaggtggg ttaaattttg
    16801 atgaagaata tgtgacgtat ggtaatggtt attcacgatg tgtgacagtt tatcactaca
    16861 caaaaaatcc tacgcttttt tggttggcca atctcatgaa caatcctcat acagtggcca
    16921 cactcgatgt tagcacagat gaaaagcaaa aagtgattga tcaaatcaat cgtgccttag
    16981 atgaattaaa tgatcgatca gataatgaac gaaaagcgac tgaccgaaat aaaagcgcag
    17041 aagaatacct ggaactagcg gaatatgctc gtaaattgac ttctaatggg gaaatttcta
    17101 agcaagtcaa agtgagagta catgtttacg gaaacacgat tcaagaagta gatgaacgag
    17161 cgcaggaact gatcgaaaat ctaaaaggga tggattatcg tgcggtgact tatctgcata
    17221 aaaccaaacg agattggcaa tcgatgttta ccatgttcga tgaacaagct gaatggttcg
    17281 gaaatatcaa agggcaatca ctaccatcgt taaacattgg cggaggtttc ccgttccacc
    17341 accaaacgct aaaagatcca ggagggacac aatatgggga aaccatgaca ggcggcccat
    17401 ttgtctggaa tgcgacgtat aaaaatagtg tacggttgtc tactaatacc ttgcttctag
    17461 gaatgatggg agctggtaaa tcgaccgttc ttaaaatgat cgcggaagcc catttagctg
    17521 caggagactt tatttggggc tttgaaaagg gaaaagactt tattcctttc ttaaaggaat
    17581 ataacggtat tatggtgcgt ttagacggat ctgacggaat gatcaatcct ttagagatat
    17641 ttgccacacg gacgtatgat gaagcatcca gtttatatga cgatggatca gtcaaagatt
    17701 tgaagatcaa tgaagcagcg tcctatcaaa cacacttaga caaagtggtc tatcaggtac
    17761 agttagtgag tcctcagcta aaaggaacga tgaaagcaga atttaagacc tacttaaatc
    17821 gtttttatga agagtatgga acagtaccac gcggctttac ttcctcaaat tcgcgttcaa
    17881 atacggagac gcaagtcaca ggtaaagatc cagaagctta tccgacgttc aaagaatttt
    17941 tagacttttt aggacaactg gaattaccag gtgcttccca agagaaaaag aatcgaaaag
    18001 aagaaatgga atccatcgta gaatccttat gtgagacgta tggcatgatt tttgatgggc
    18061 atagtacgat tcgacacctt gaccaacaac agttggtctt ttttgacatt gactctatta
    18121 caggcttaga tcgaggggta caacaatgtc aaatgtacat ggctatgacg ttgatttgga
    18181 atcaggcatt gattcaagga agaagacaac gacgtttaat taaagaaaaa caaatggaag
    18241 aacaagactt caaacgcttc ttggcgatga ttgatgagtg ccataatgcg attaatccag
    18301 attttattga aacggtaaat tatgtcacaa actttcaacg agaaatgcgt aaagtagaag
    18361 caatgactgt tttagctaca caatccccac aagaaatggt gccagaaaat atgtcttctg
    18421 ataacttctc gaaactgaag aaagtctttg aattttcttc tacgaagatc tttatgcgta
    18481 tggatgaatc tattttacgt catatagaag gattagtggg taaatcaatg acgaactcag
    18541 aattagaatc cattcctcaa caaaatcaag gagacgctgt gatcaatttt ggctcaaaag
    18601 aaaccattcg tgttcatttt agtccgaaca aacgtcaatt aaaattgttt gatggaggaa
    18661 aataatggaa aaccaaaatg aaagtttaat caaacagtat gttaagcgtc gtgcaaagcg
    18721 gcgcttattt ttgtgggtgt ttggtacttc tgcaggtttg attactatat tgatcactgt
    18781 gtttgtgacc ctttttctaa tacttgcggc aggttctatt gataattctg attcaagtag
    18841 tggtggcgaa gcttttacag gagaatattc ggaaggctta cccatttata aagaaatcaa
    18901 aggaagaggc cctttttctg atgagattgc gcaatatgct gtaggtgcag cagtgaagta
    18961 taaattatta ccaagtgtga tcctttcgca atacggttat gaatcagcgt ttggtacgtc
    19021 ggcgtccgca agaaatgatc tcaattattt tgggattacg tggtttgatg ggtgtctgtt
    19081 tcctaaagga actgcgcgtg gaattggtgg cattgaaggt ggctggtaca tgaagtttcc
    19141 caactcaaaa gctgccttct cctattacgg ctttatggta gccacacaat caaattttaa
    19201 tgcttgtgtc ggaaataaaa gccctggcgc aagcttattg atcttaggac gaggagggta
    19261 tgccgccgca ggaattactg aagacagtgc ctattataaa aattgtatgt ctattattaa
    19321 tacaaataaa ttgatggagt acgatgagtt tgcgattaaa cactggagag aaggcggcgg
    19381 aactggtggt acgattacag gaagctgggg caatccattc ccaggaagta gcttagataa
    19441 aaattcgttc agtggaggac aactttttgg gaaaaatcca ggaggagaat tccgacctaa
    19501 cgggttccat gatggactag actttggttc agtcgatcat ccaggaagtg agattcatgc
    19561 ggttcatggc ggaaaagtcg tgtacgttgg caatcctggt atttcaggtt tgggtgcgtg
    19621 tgtgatcgtg attaattacg atggcttgaa tatggtgtat caagaatttg caaatagtac
    19681 aggaaattct cgagtgaaag taggcgatca agtgaaagtt ggtcaagtaa ttgggatacg
    19741 agatacagca cacttgcatt taggatttac tcgaatggat tggcgtcaag cacaaggaca
    19801 tgcattcatt gatgatggta catggatcga tccgttacca tttttgaata gcagcaaaaa
    19861 atagatcagg agaaaaaata tggaaaataa aaccttaaaa atcgtcttaa cagctgcaat
    19921 tagcgttagt ttaatcggca atattgcgtt gttttggcag cttaaagaag aacaagagac
    19981 aaatacctat aaaattgaag aagtggctaa aaacgcgtct gataagctca aaaaaaattc
    20041 ttctgaggca caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga aaaaccaaga
    20101 aatcgatcag ctgaagcaac aagatggcac aaaagcaaaa gaggaagtaa ctgaagcaca
    20161 aagaaagacg gcagaagagt tcggtcaatt ggccattgat aaatcattaa gcagaaatga
    20221 attaacggaa aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat taagtcctgc
    20281 agacgatgat caccaacatg acgactacgg aagttactct tttacattag gtgacgtgca
    20341 aacttacgct caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat ttgttgatta
    20401 tacaatcggc aatcctcaat ttaaagatgt caaaccacaa aaaataaaag gtggattaac
    20461 tgtcactgaa aaacaagtag atggtcaatg gaaagtcaca gactttagtt atttcactcg
    20521 ttaggaggaa gaaaatgagt aagaaaaaag gagcaagaca acgttttaaa aaacgaaaaa
    20581 gttatcttag atggctgggg atctttatga ttctcggctt tttgattctt gctagtccga
    20641 tttacacaca taaaaaggta gacataccag atgaagtaag cacgaacgca tttttaaaca
    20701 cgaaggaact gacgatggtt tcaaaagaaa aaggagcgaa ccagcaatta gtttttgaat
    20761 ttgcgatcga cgatgaagat cagagcttat taaaagaact agctaatctt aattatcgtg
    20821 tagaagtaaa gacagctaaa ggggattatc aagcaataaa agccaaagtg atcaaggtgt
    20881 ctgatgatta ttttgtagtg aagttagcac atgtaccaga agaatatatc gccatgcgtt
    20941 taaccattat tcctgaaaaa attgatccaa aagtggatat gcaagaacca caagatctga
    21001 tttattacgt tcacgaagat aaggttaaag atcaagtgaa agatgaagat tatgaacaac
    21061 atgcgatcaa ttacaaagtg aaaggataca aaaaagaaca aaaagcagca caaaaacaaa
    21121 tggaatcctt ccaagcaacc attgatttga atgaagaatt agtgaagaag ctaaaagatc
    21181 aattgcctta tcaagtcgca gatgatcagg aaaatactga aaacaagatt aatagctacc
    21241 agcaagaaat tgaaacatcg aaaacgcaaa tgaaagatca agaagagatc attcaaaaac
    21301 ttcaagaaaa aattgatcgt atgacaaaag aaaagattta gaaggacagg tggagtgaaa
    21361 aaatgaagcg aacaaacttg gctgacaaag atactttcgc tgagcagaaa gtattgatta
    21421 aagcctatct caaagttgta aaagctaaag aaatatgtcg agaagtgaat ttatctaata
    21481 caacaatatc tgagttgaag aatggtcgaa agaatttaga tactttaaaa ttcagatcat
    21541 ttgaagctct atatgtagct gctaaaaaat attttacagc tgataacaaa taaaaaacag
    21601 gagtatttta tgtgaaaaaa gtatataaaa aatgcttatg aatcagctat atcaccttaa
    21661 ccatttgtta atgagatggg agaaaacatt aaccacgatt ctaacgcaaa catcaagcac
    21721 ataaaacatt taacactcct cctttctggc tgaagatttc aaccaaacag gaagacagcg
    21781 tatcaattat agcatacact ttttcttaaa ttcatttttc atatgacaaa atatttcctt
    21841 ctaatcgaca aagttagaag gattttttat tgtctaaata cacaaataac cttaatttat
    21901 aaagagcata aggaaacgtg ggaacacttt ttaaaaaaag tgttccaata ggctgtgcct
    21961 attacgtttc tcaaaaataa ttgtgggaac aaaatattta aaaataggat agtgaaagga
    22021 tagcaaagga tagtaaaagg atagaagcag gatagcgaaa aggataggct ccttttcgct
    22081 atccttttta aattggagga attaatatgg aagtaagaat aagaaatgta gatgagaaaa
    22141 tcgtaagaga aattgatcga agatgtgacg aactaagtaa ccgaacgggt aataaatgga
    22201 gtcgtaacga ttatttaaag ctactcgttg aaaatgattt tgatcgtcct ttgatggaat
    22261 ataaacaaga taaatttgat caattattaa atcagttaat agataaattt tcttttgttc
    22321 aaagtcataa tacaaaagta ttagaagaat atatttgtca gaataatcaa attatcgaac
    22381 ttttaattga acaaagtaga ggaggagaag tttaacatgg ccaaagttat agacaagcat
    22441 atttattttt atgaaacaca tatagatgtt ctagatgaaa ttattgcaac tcatgcagag
    22501 gttaaaaact atagtgaagc cgttcggttt ctatgtatga attatgattc agaagtggag
    22561 aaaaaagtaa attccaatca agcaaagttg aatgcaatgg gaaaagaaat ttctatttta
    22621 accgaaatgg tttcagagtt tgcggatctc catttgaaag atacagggat tttaattggt
    22681 acaaattcaa ctgtttacca agaagcaaaa gagcgtgtgg aaggcaagat aaaagcaaat
    22741 caaacgaaga aatattctcc taagataaag aaaacaataa gcgaatcagt gactacaaaa
    22801 agtgaagtat ttgatccaac aaggttctta aaacgataaa aattagaagg agaatgtgat
    22861 gagtaaatca cctgcagtca ttctcacttg tcaatttacc ttgcccaata aaaaatcttt
    22921 ttcaacatac attgattata tgacaagaga aaaagcgctg gaagaaaagg aacgacgtac
    22981 gcccgaggaa gaacaggaac tgcgtctaat caagaatgct ttagaggatt tttatattcc
    23041 tgaaggagaa actttttcgg agagtaagac gggtaaagaa ttcagcgaaa cgtcgaatga
    23101 agcgcaatcg ttgatgaaac atggaatcaa ttttgacaag ttagaagaac aagattttac
    23161 caaatatctt tcttatatga cacgtcacta tgcgttagaa caaaagaaag tactgacgca
    23221 tagtgaacaa aaagaatacc aacgcttgaa ccaagcaata aaaaaatatc aaacagagcc
    23281 acacaaaaaa gcttcaggaa atcttcctgg agttttttct atggcttcag acgaagtgaa
    23341 aggaagtgat ctgaaggaga tcaagcaaac cttccaaaga ggacaacaaa aaggctcaat
    23401 catttatcaa gatgttgtct ctcatgatca ttcgtatctt gaaaaaatag gattgtacga
    23461 tcccaaaaca gatactttag atgaagaagg tttaaaagca gctggaagaa aaatgatgga
    23521 gacattgttc gagaaagagc aactgaatga aacaggctac tggatggcaa cgattcaccg
    23581 aaataccaaa catatccata ttcattttgc agttgttgaa aaaaaaaata cgcgtaaacc
    23641 atacactgag attgaaaatg gtgtgagtta tatcgtaccg aaagggaaga gaaggcaagc
    23701 aacattagac gaaatgaaga ctaattatgt acatgagtta gaacgttttt cttatgaaaa
    23761 aaatcgaatg ggtatggaga aacgaaattt attgacacgg aaaagtgatt tgcgtaacac
    23821 cttaaccaaa gtagtgaaag agccagtacg ttatgatcga cacgcaatga gcttattaac
    23881 tgaagtatat aaaaacttac ctaaaaagcg ttcggaatgg cattacgggg atgaaaatcg
    23941 aaccaaactt tcaccataca caagagagaa actcgatgag ttaacccaat atgtgttaac
    24001 aaaagaacct ggatatcaag aatatataca gatttcaaaa gcattgaaag aagaagcaaa
    24061 aagtctttat ggggaaagca agagagaatc aaaggatgca gaaaaaaatg ctctctttga
    24121 tttaaaaaag cgcacaggaa atgccattct aacagaactg aagaaacagg atagtcaaat
    24181 aaaaccttta atagaaagga tggatcagat gcaatctatt tacaatgaac gatcctttcg
    24241 aacacttaaa cagttgccaa cgaatgaata caaatatagt tacgaagaat tcatgaagcg
    24301 caggaaacaa ttccaacaaa gagttgtcac gcaacggagt ttgcgtcaat taaatcgaac
    24361 gattaatgcc catgaagaaa aataccacgc aagtaaagca tacgaagagc aacaacgaag
    24421 aattcaaatg gaacaagaaa gacagtagga ggaatagcaa tgaaacaata tttagatcaa
    24481 tggaaagtta tagaaggatc attaagagaa gaacgaatcg aacaacttcc cgattgttta
    24541 gaaaaagagc atttgtttca gataagagaa atgttgataa acgaacagtt tgatccaaat
    24601 caatttttag tcgttgagta cccagctact ggtgtgtact gttgtaatca tgtgaagggt
    24661 gaaaaatatt ttatcattca agaatatgag ggcaagttag cgccttatta tactacttgg
    24721 gaaatgaatg aagaggggat caataacttc ccgtgtaaaa gcattgaaga gtctatttca
    24781 ctcacagaat gttagttgtt aagtgaataa aagaagtaaa agagtcctga acgtgattgt
    24841 ccaggactct tttttttcac tgaattgttt tccgccaatt cattgagtgg tgcgatttcc
    24901 gtctacatta tatcaaaaga aaggaggaag aagaaatggc ttattcaacg attggagttg
    24961 ttgttgaaaa aagcagagac aatcttgttt ttgtgacaga aattcaaaca ggcagagctt
    25021 ttgtagtcac agacaaagcg gcaaaagcat atcaaagtgg cgatatttta acgttaaatc
    25081 tgactacgaa gacgttcgtc gatgcggcag aagattatcc att
//
LOCUS       DAYYKN010000030        24255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000030 DAYYKN010000000
VERSION     DAYYKN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.030"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..1337)
                     /gene="purB"
                     /locus_tag="ACHBB7_001024"
     CDS             complement(42..1337)
                     /gene="purB"
                     /locus_tag="ACHBB7_001024"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM9208990.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1350..2474)
                     /gene="purK"
                     /locus_tag="ACHBB7_001025"
     CDS             complement(1350..2474)
                     /gene="purK"
                     /locus_tag="ACHBB7_001025"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM9208991.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2467..2955)
                     /gene="purE"
                     /locus_tag="ACHBB7_001026"
     CDS             complement(2467..2955)
                     /gene="purE"
                     /locus_tag="ACHBB7_001026"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM9208992.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3371..4678)
                     /locus_tag="ACHBB7_001027"
     CDS             complement(3371..4678)
                     /locus_tag="ACHBB7_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM9208993.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4681..5262)
                     /locus_tag="ACHBB7_001028"
     CDS             complement(4681..5262)
                     /locus_tag="ACHBB7_001028"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM9208994.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5378..5475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5834..7126)
                     /locus_tag="ACHBB7_001029"
     CDS             complement(5834..7126)
                     /locus_tag="ACHBB7_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9208995.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7123..7746)
                     /locus_tag="ACHBB7_001030"
     CDS             complement(7123..7746)
                     /locus_tag="ACHBB7_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9208996.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7749..8585)
                     /locus_tag="ACHBB7_001031"
     CDS             complement(7749..8585)
                     /locus_tag="ACHBB7_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9208997.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8602..9489)
                     /locus_tag="ACHBB7_001032"
     CDS             complement(8602..9489)
                     /locus_tag="ACHBB7_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9208998.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9505..10788)
                     /locus_tag="ACHBB7_001033"
     CDS             complement(9505..10788)
                     /locus_tag="ACHBB7_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9208999.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11471..12154)
                     /locus_tag="ACHBB7_001034"
     CDS             complement(11471..12154)
                     /locus_tag="ACHBB7_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM9209000.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12172..12789)
                     /locus_tag="ACHBB7_001035"
     CDS             complement(12172..12789)
                     /locus_tag="ACHBB7_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9209001.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12799..13623)
                     /locus_tag="ACHBB7_001036"
     CDS             complement(12799..13623)
                     /locus_tag="ACHBB7_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM9209002.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13616..14176)
                     /locus_tag="ACHBB7_001037"
     CDS             complement(13616..14176)
                     /locus_tag="ACHBB7_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209003.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14350..15306
                     /locus_tag="ACHBB7_001038"
     CDS             14350..15306
                     /locus_tag="ACHBB7_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9209004.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15611..16567)
                     /locus_tag="ACHBB7_001039"
     CDS             complement(15611..16567)
                     /locus_tag="ACHBB7_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM9209005.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16671..17273
                     /locus_tag="ACHBB7_001040"
     CDS             16671..17273
                     /locus_tag="ACHBB7_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9209006.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17361..18860)
                     /locus_tag="ACHBB7_001041"
     CDS             complement(17361..18860)
                     /locus_tag="ACHBB7_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM9209007.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18876..19400)
                     /locus_tag="ACHBB7_001042"
     CDS             complement(18876..19400)
                     /locus_tag="ACHBB7_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM9209008.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19698..20447
                     /locus_tag="ACHBB7_001043"
     CDS             19698..20447
                     /locus_tag="ACHBB7_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9209009.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20438..21223
                     /locus_tag="ACHBB7_001044"
     CDS             20438..21223
                     /locus_tag="ACHBB7_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9209010.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21192..21908
                     /locus_tag="ACHBB7_001045"
     CDS             21192..21908
                     /locus_tag="ACHBB7_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9209011.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22010..23029)
                     /locus_tag="ACHBB7_001046"
     CDS             complement(22010..23029)
                     /locus_tag="ACHBB7_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM9209012.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23048..24049)
                     /locus_tag="ACHBB7_001047"
     CDS             complement(23048..24049)
                     /locus_tag="ACHBB7_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM9209013.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7620 t
ORIGIN      
        1 ttgctattct attttaattg aattttgcaa ctcactcttc tttattccaa acctactcgt
       61 ttgaatatca cgtcaacgtt tttcaaatga tgatgataat caaaggcatc gtctatttct
      121 tctttagata ataccgatgt gatggttgga tcttcttcta acagcgggcg gaactgcact
      181 tggttgtccc aagcgtacgc agtttttggc tgaacaagat cataagcttc ttctcttgtc
      241 attcccttat cgatcaattt cagcataacg cgctggctat aaatcaaacc aaatgttgca
      301 gacatattcc gcttcatgtt ttcaggaaag accgttagat ttttaacgat attgccaaaa
      361 cgatttaaca tgtaattcaa taaaatcgtg gtatcaggca agatgattcg ttctgctgaa
      421 gaatgagaaa tatctctttc atgccataat gtgacatttt cataggcagt gaccatgtgt
      481 ccgcgcacca ctctggctag accagccata ttttctgaac cgatcggatt ccttttgtgc
      541 ggcatagcag aagaaccttt ttgacctttc gcaaaaaatt cttctacttc acgtgtttcg
      601 gatttttgca atccgcgtat ctctgttgca aaacgttcaa tacttgtagc aataagcgcc
      661 atcgctgaga aatattcagc atgtagatcc cgagggagaa cttgagtaga gatttcttgt
      721 ggacggatcc ccaattgttt acatacatac tcttcaataa atggcgggat atttgcgaac
      781 gttcccactg ctcctgagat ttttcctgct tctactcctt ttgccgcatg ttcaaatcgt
      841 tcgatgttcc gtttcatttc tgaataccat gtggcaagtt tcaatccaaa agtagttggt
      901 tcagcatgga caccgtgtgt ccgacccatc atcactgtat atttgtgttc ttttgctttt
      961 tcgccaatga tttccgtaaa acgtttcaag tcttcccgta aaatatcatt tgcttgcttg
     1021 atgagatagc cataagcagt atctacgaca tccgtacttg ttagaccata atggacccat
     1081 tttctttctt ctcccaatgt ttcagaaacc gctcgtgtaa acgcaacgac atcatggcgt
     1141 gttaattttt cgatttccaa gatccgatcg atatcaaatg atgcatgctc acgaattttt
     1201 ttcacatctt cttgcgggat ttcaccaagt tctgcccaag cttcatctgc taaaatctcg
     1261 acttcgagcc acgcacggta tttgttctct tccgtccaga tattccccat ttcagggcgt
     1321 gtataacgtt ctaacatgtt ttttctcctc taatcccaaa tattcgtttg tttgatttct
     1381 tccagcgttt gctggatgtc ttctgtcaag atagtgatat gtcccatctt gcgacctttt
     1441 tttgcttgtt tttttccata gaaatggaat tgccagtctg gcttattctc aatcaagcga
     1501 tagctttcca gcagttgttc tcccaaaaca ttgaccatta ctgcattgct tatcaaacgt
     1561 actttttctc ctaatggcca accacaaata cctcgaatat gggcatcgaa ctgactcata
     1621 gaacaagctt caatagaata atgtccagaa ttgtgaggac gaggagctaa ttcattcaca
     1681 tatagcccgc ctgtttttgt taaaaacatt tctaccccta aaacacctga aaggctcact
     1741 gcttctgcaa tcacacgggc aatacgctca gcctcctcga tcacatcctg atcaatcgcg
     1801 gctggagcaa tcgtttcatg aagaatattg ttgtggtgga tgttttccac gaccggaaaa
     1861 gtcgtgaaat ctccttgacc atttcctgcc accatgatcg aaatctcttt ttcaaacggg
     1921 atccatgctt ctaaaacaca ggttccttct cttaaaaggt ccatggcagg agctagatcc
     1981 gcccgacttt tcaaaacata ctgccccttg ccatcatagc cgcctcgagt ggttttcaaa
     2041 acacaaggat agccgatccc gtcaatcgca tcttggatat ccgtcggact gacgatcgtt
     2101 gcataaggag caatcacgat gttattcgtt tccaaaaaag atttttctaa caggcggtct
     2161 tgcgtaatcg caagcaagtc tgtcccttga ggaataaatg acatgggtaa aatcgcattt
     2221 aacgcctcta cactgacgtt ttcgaattca taggtaatga catctgttct tctggcaagc
     2281 tctttcagcg caaaggtgtc atcatagaca ctttcaatgt gccaatctgc aacttgtgcg
     2341 gctggacaat cgctcacagg atccagcaca cctactttaa agcccatttc ttttgcacta
     2401 atcgccatca tacgacctaa ttgaccgcca ccgacgatcc cgatcgttga accaggcaac
     2461 aaaggtttaa ccaagttgat cactactttc tataacagtt ttagccaaca ttgaacgtct
     2521 ttcagccaat ttagcctcca attctaaatc atacatggaa agcatctgta tcgcaagtag
     2581 tccagcatta attgctcccg cttttccgat tgctgtagta gctacaggta ctcctccagg
     2641 catttggaca atcgaaagaa gcgaatccaa accatttaat gttcgtgatt ggaccggtac
     2701 accgataacc ggcaaagttg tttttgatgc gaccataccc ggcagatgcg cggctccccc
     2761 tgctcctgca ataatgattt tgattccttt ttttcgagcg ttttgtgcat attcaaacat
     2821 caaatctggg gttcgatgag cagaaacgac ctttttttca taagacacac cgaattcatc
     2881 caatatttga catgcttctt tcattgtttc ccaatctgaa gtactcccca taatcacaga
     2941 gatttttgct gtcattgttt ttcctcccag tttttctaca ttcatatttt aacaatcact
     3001 ttttttgatg tcaaagaaat ttcgaatgtt aacaaagaaa aacattttat tgttcgtgtt
     3061 tattaaagat aaaaaaaagg aatgtgacaa aagttaggtc acattccttt ttatggataa
     3121 acggtgttct cctgccaact cctcggtcac aaggaacaat attcaacaaa catgagaagc
     3181 ttttttttga aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttcct
     3241 tcaacggtgt tcaatcagct gaccctcggt attagctcaa acaaacggca aaatacgagg
     3301 aactattttt ccgtttgttt ctctaacaat caggtcaaaa cgctgatttc acaacctttc
     3361 ttttgctcta ttacttcttg aaggcttctt ccaatatttc ttcatctttt ttcaatccat
     3421 taaagatcaa attcaatatg atagccgtaa tgctactcat tacgatgcca tttccagtga
     3481 acatttgcaa tgtttgcggc atttgactga ataatgtcgg cgtaacattg aaacccagcc
     3541 caaatccaac agaaacagca acaatcaata gatttttatc attgctgaaa tcaacttcca
     3601 acagcatacg gattccttgg actgccacca tcccgaacat gactaacatt cctccaccaa
     3661 gtaccggctc agggatgatt tgtgctaacg caccgatttt agggaataat cctaaaacaa
     3721 tcaagaagaa tgcagagaaa tagatgggtt ttcttgtttt gattcccgaa agttgaacca
     3781 atccgacatt ttgagaaaag ccagtataag ggaatgtatt gaaaataccg cctaagataa
     3841 cggcaagtcc ttctgcacga tatccctttt tcaattcttc ttctccaaca tttttcccag
     3901 taatgtcacc aagtgcaaaa tatacacctg ttgattctac catgctgacc atggaaatga
     3961 tgatcattag taaaatcgac catacgtcga aagttggttt accaaaataa aagggttgtg
     4021 gaacatggaa caacggcgca tctccaatcg cgctcagatc aatcattccc atgaaagcag
     4081 ctaagatgct tcctccgact aaaccaatca ataccgcgat tgaacgaatg aacccttttg
     4141 ccagcatttg cacgcctaca attaggagaa tcgtcacaaa tgcaagtagc agatttgttt
     4201 tgtcaccaaa atcagctgct gttgcattac ctccacccat tttctcgatt gctacaggaa
     4261 tcaaagtcaa accaataacg gtgataaccg ttcctgttac tataggaggg aatagttttt
     4321 taattttaga aaagacacca gaaactaaca caacaaagat cccagaagcg ataatagatc
     4381 cgtagatagc tcctactcct tcattggttc cgatcaagat cagtggagct actgcttgga
     4441 tcgcacatcc taaaacgaca ggcaagccaa ttccgaaaaa tcggttaact gtcaattgca
     4501 ataatgtcgc taatccacac ataaagatat caattgaaat caaataagtc atctgttctt
     4561 gattaaagcc tagtcctgta ccgatcaaaa gaggcaccgc tactgctcct gcatacatcg
     4621 ctagcagatg ctgcagccct aaaaccgcag cttttccatt ttgtgtttct ttttccacga
     4681 ttatgcgtcc tcctcaagaa actcaacttg tccattcgtc aaggaagcaa tacgcgcaag
     4741 agaaacgact ctcaatccca tttcttcaag taactgacgt ccatcttgga aagacttttc
     4801 gatgacgatc ccgattcctt ctacttgcgc tccagcttgc tggcataatt cgatcaatcc
     4861 ttttgccgct tgtccgtttg ccaagaaatc gtcgataatc aatactttgt cttctgaaga
     4921 taaaaattta cgagaaatag aaatcgtact tgtcacttgt ttcgtaaaag aataaacaga
     4981 agctgtcaat aattcttcat ccatcgtcaa actttttgct tttctagcga agatcattgg
     5041 aacatctaat tctcttgcag caaataaagc tggtgcgatc ccagaagctt caatggtcac
     5101 aactttcgtg attccttgtt ctttaaatac ttctgcaaac cgttttccca tttgttccat
     5161 caatacaggg tctacttggt gagtgacgaa gctgtcgact tttaatacgc cctcgcctaa
     5221 tatacgtccg tctttttgaa tacgttcaac taattctttc actttatttg ctcctttatc
     5281 atttattaat accgaaataa ccgcttttgt gcaaaaacct actatgtttc tgttaaagaa
     5341 aaacaccctc cactttatca taagaaaaga agggtgaaat agccaatctt ttacttatag
     5401 tccagcattt acggtactgg ctagaaactg tcatccatat tatgacgata tataagtagg
     5461 tatgtaaaac taataatagt gtaaattcat tttcttgcaa ctgttttgca cgtgaaggca
     5521 gtttgccata tttgaataaa aaatgttgtt tcaaatattg aaaacagtct aacactactt
     5581 tctgaaactg acaacgtggt ttacttattt ttttgtaaaa tctaatgatt tttaatgcat
     5641 tattcctttt tgttcgggtt taaatagtta tttttatttt ctcctatcta aaaaagcagc
     5701 ccacataaaa agaaggcatg acaaaaagca tgtcatagcc cctttttccg aataaatagt
     5761 gttcaaaaga cagcctatcg gcaataaggc aagattttat aaactacggg aagtaatttc
     5821 cgtaaatcta ttcttattct tttaagctga caagctcgtc aatcactact gcttttcctg
     5881 aagcaataga atgatttgct gcgattcctg tcaaaatcga caaaatacca tctacgtgag
     5941 ttgctgcccg attccacgga tcttcttctt tttcttcacc aaatagatca tctaataaac
     6001 gcggatctcc tccgccatgt ccgcccgctt cttgttcgac gattacctca tatggctcac
     6061 cgaacatcgg atagacatgg atggactgaa gaacggctgc tccttcatcc tttttatctc
     6121 cacctgcatt gatgtaagac ttttcgatca ctttcatttc gatccgtcct ttgctgccat
     6181 tgaaagaaac attgaatcct tcccacggca tatacgcgtt tagcgaatac gtcaaaatcg
     6241 caccagtttc atatttgacc gttacgccta gtgtatcttc aatagagata tcatcggaaa
     6301 atacactgcg gtcacgaata tatccactgt ctttttctgc ttctaaatac atttctcgca
     6361 attcctcatt ggctgacagg tctatagcaa atggatcagc tttagctagt tcgcttccat
     6421 agcatcgact gtaaaatatt tctactccgc gtttctccgc attatgcatc ccataaaagt
     6481 ttaatccacc gaatgcaaat accttttggg gacggctgtt caaccaaaaa ttcactagat
     6541 caaaatgatg ggtagattta tgaacaagaa gcccgccgct attgtgctta tttcgatgcc
     6601 atcttctgaa ataatctgcc ccgtgttgcg tatttaacag ccattcgaag tggatagaaa
     6661 acacatcacc aatcacatca ctttctatca gttcctttat cttcgtgtga tacggtgcat
     6721 aacgataatt aaaagtgact cgcaggcttc ttcccgtctg tttttggacc gttattattt
     6781 cttgagcttt ttcctcatcg atagtcagag gtttttcgca aataacatca tatccttttt
     6841 tcattgcacg aatgatataa tcatgatgcg ttcgatctac tgacgtaata atgacttcag
     6901 ccggccgtac gtcatccagt agttgctcaa attgtgatgg catacatgtc tttacttttt
     6961 tctgtccata cttgttcaca agtatccgat ttgcataatc catccttgtt tgagatacat
     7021 cacaaaaacc gactagttca caagtttgat gatacgtaga ggcaattgcc tcataaaaaa
     7081 aacgcgctct cccaccaagt ccgacttgta catatttttt cttcatttaa agtcattctc
     7141 cttttctttt aagcaaaata ctgtgacaaa tggcagccct ggatacagta atgcagtgag
     7201 taaaaaatca tgctgtatac accagaacag tccatacaaa aggcataaat accacagaaa
     7261 ccccttgacg ttttttgaca acagccagat attccttttg atcaaataaa caagactatc
     7321 tgctttatct tctttgcaga tatcaattag cgtactaaaa aaactatggg atacaaaaag
     7381 caacagacac aatagaaaag gaatcagata ccaagctcca atctttccca ctaataaata
     7441 ccaatcaact gcaaaaataa tgacaaacag ctgaaacaac gtaatgatga aacacatccg
     7501 tttgattttt cctttcctcc aacatacctt tttttctgcc aagtaatgga cagatagata
     7561 ataagaagga tacatcatca aaaaataaat cagatagaca acacgagcag acagcactaa
     7621 tcctaaagtg aacaaacatc ctagaactaa agcgaaaaga gcattactga taagactggt
     7681 taaaaaaatc cgacagacat agtagttgag cttgtaaaga aacatctctt ctattggctt
     7741 tttcatattc agcccttcag accgcttgta ctgatcccct ggacaagata tttattgaag
     7801 aataagaaaa tcagaaaaac cggtagcagc gacaacgtag acatcgcaaa catactcgcg
     7861 taagtcgtac tagatccagc gtctgcaaat aatttgatgg caatcgatac cgtgtaagat
     7921 ctcggcttgt ttaaataaat caatggtccc atgaagtcgt cccatttcca ataaaactgg
     7981 atgatgatcg tcgtgatgat cgaaggtttc agcaaaggaa aaataatttt ggtaaagaca
     8041 ctatacttgc tgcatccgtc aataatcgct gattcatcta actctttggg gatgctgacc
     8101 atgaattgca tgatctggta aatgaaaaat gcttgtccaa aaaaatgtgg taaaatcagc
     8161 ggcacatacg tcccaaccaa attcagccga ttgtaaataa taaactgcgg aatcaaaatc
     8221 acttgtgcag gaatcatcat cgttactaac atgacgacga accagaatct ttttcctttg
     8281 aaatcgattc tcgataatgc gtaagcaata caagtcgagc tcagaactgt ccctatcgtc
     8341 gaaactgcag agatgacaaa cgaattccta aagaaagtaa aaaatgaaat tccagcaaac
     8401 cccgcccatc cttttgcgta attttcccat tgaaaagtct ctggaatcaa attcagcgaa
     8461 ccactcataa tctcttgatc cgttttaaaa gaacctgcga tcatccataa gaccggataa
     8521 atcatgacta gacctaatag caaaataaaa ccgtattgaa accatttcgt tactgtatta
     8581 ttcatttctt cctcctctca ttcacacaac gacccatttt tttgatgatt tgaaaatcag
     8641 aaaggtgaca gctcccataa tcaacagcat gatccacgaa atagcactag cataccccat
     8701 agaattaaaa gaaaaagctt ggttatacac ataaagagca taaaagttcg tggcatcatt
     8761 cggaccacct tgggtaatga taaatgcctg cgtaaaagtc ataaacgccg cgattgtctg
     8821 cataaccaag ttatacaaaa tgatcggcga aagaagaggt aacgtgatct tgtaaaacgt
     8881 ctgccatttg ttcgctccat cgatctctgc cgcttcataa tacgagcctg ggatctcttt
     8941 gatacctgca gcaaagatga tcattgccga accgaattgc caagcagaca tcaaaacaag
     9001 agggatcatg accagttttt gatcgctaaa ccaggaaagc gcaggtgcgc ctaatcgttc
     9061 caggaaaata ttcactaacc cgtttttaga aaacaactcc cgccaaacta gagcgataga
     9121 aatactgcca ccgatcaaac taggcacata atacaaagaa cgaaagaaag tcgttccttt
     9181 gatattcctc gttaaaagat aagcaatcaa tagaccaaag atcagtttca caggtacaga
     9241 tgcaaataca tagagaaaag tgactcggat ggagttcaaa aaacgggcat cactcagcat
     9301 ctttatataa ttatccaagc cgatccatga gactggattt cccaaacgat aatcggtgaa
     9361 cgaataataa agtgacaaga tcatcggtat cactgttatc aatagaaatc ctaggatgaa
     9421 tggagaagca aatacgtaac cgaccacatt gtcttgatcc aagaaccgtt ttagtttttc
     9481 ttttttcatg ccttcctcct gctgctatcc tagaatgttt gacgcaaact cataaagact
     9541 ctctgctgct tcttctgctg ttacttcttc ataaatgact ttttcaacta aaagattgta
     9601 ttggtcttga atttccggat tcaaagcact atcaaagaca ttcacttcgc cgttgtccat
     9661 atcggaaatc atttctaaat aatcaaatgt cgcttgcaaa gttgcatcat ctgcacgagc
     9721 gagctgttct cgaatattcg acatgattgg tacaccacgt tctcctttta aaatttcatt
     9781 ggcttcttca ctgttttgaa agaaatcaat aaacttagca gcttcttctt tatgggctga
     9841 gctttttgcc atactcagca tttgcgaaga acgtaaaggg ataccagatt ctccatctgc
     9901 ttttctgcga ggtggattaa tcaactgaat ctccatatct tcaggagccc ctgacaatat
     9961 ttctgtcaac tggttacttg atagaaacac cattgccgct tctctatccc taaccgcctg
    10021 aaaagacaga tccgtcgtac ttgcagctcc accacctggt tctggatggg cccctttgtc
    10081 taccagattt ttacgcagct ggaaaaaatc tgttagatag tctggatttt caaatcccaa
    10141 gctgttagat agatcttccc gattataaaa attcaatcct ttctcaactt gtggtatttg
    10201 aataatacag ccagctataa aatcttcgaa tcttgaaata gcatagattc ctaattcttc
    10261 agtgatttga tagctgattt gttcaaattc atcccatgtc caatttgaag ctggttcagg
    10321 gatatttaat tgttcaaata tatttttatt gtaagcaatc gcaaacgaat tcaccccgtt
    10381 agacagtcca actaattgcc cgtcgtaagt cgtcgttgct aaataggatt catctgcatt
    10441 agaaatatca attatccctt gctccacata ttcatctaat ggttcaatct gattgatgta
    10501 ttgaggaaat tgattgttca gttggaaaat gtcccagata tcatctgatg ctgctagtgt
    10561 acctaatttt gtgtaatatc cttcataatc ataaaattca taatcgatcg tcacatgggg
    10621 atttttttct tcataaagct cgattacttt tactgttgca tcgtgtcgga cttgcgaacc
    10681 ccaccaagcc atgttcagcg tgattttttt gtcatcttct tgcccacaag ccgtcaagaa
    10741 gaataacata acacttaaag ctaagatgat tctcattctt ttcttcattc tctgcacctc
    10801 tacttccatt gttatttttc aatatactgt atttttatac agaacatatt tattaaacga
    10861 tctttttcaa ggggaataaa aaccaagact tgccttatcg ttctgcctag gatgaacaac
    10921 tcaatacttt caagaaaaat tagatagtgc tgattcttct ttcatcgcta aaaacacttc
    10981 ttgcttcaat gatgggagga taattacgca aagaaaaaag gatatcagac aactgcccaa
    11041 tatccttgac atagctaaat aagcatgctt cttcgtgtgc gctcttccct aaaaaacaga
    11101 aaaataacta gtaattctgt atgaaattag gtacaatata cacgtcgatg cacaagcatt
    11161 gactatggca acttgattgg tcagttcccg ctgacttttc aagccccgac aatttagctg
    11221 ctactaaatt gtcgggtgcc ttttttagtt tttattcgtt tttcattcta tagaaagcgc
    11281 ttcaataaat caagacattt ttttgttaga atgctaataa taacaaaagg ttttaactct
    11341 tcttatgtac attacaggta aaaaaacaaa aaaatttctc ttttttttga attctgaaca
    11401 aatagtactg gagtacaaaa gacgaggaag agccactatc tgctcttcct cgtcttttga
    11461 ttctcacctg ttattcatga ttctttttct cagtttcccg atatttttcc attttacgac
    11521 taaaaagctc tccgctactt ggtggcgtat atgtgatggc atttgctcct gcttgaatgg
    11581 tctcagcaat actttcttcc gttggaccac ctgtcgcaat gataggaagt tcaggatatt
    11641 ttttacgaaa atggcgtact gcttctgcag tttctttacc tgcactgata ttgataatcg
    11701 aaataccagc tgctaacttt tcatctattt ctgtatattt cgacgttacc gtactgataa
    11761 ctggaatatc aactactcga caaacttctt ttactgtctc taaaggcgta ggaccgttca
    11821 aaacgacccc taacgaacct tgcgcttctg cgaacatgct catgtaggaa gaacgagcac
    11881 cttgtgtcaa accaccacct acaccggaga aaactggaat gtcagctgcc tcaataatgc
    11941 ttttagtaat cgctggatga ggtgtaaaag gatacacagc aattactgca ttcgcatttg
    12001 tattacgtat gatcgcaata tccgtagtaa agatgatcga acggatcaat tttccaaata
    12061 tcttgatccc gcttgcttct aaaatgattt ccggtactct tacgatatct tttctcaatt
    12121 ccgtcatgat ttctggcgta aattgttctg ccatcagcca cttctccttt gctatctccg
    12181 gtcatactga ttcaattgat acgtctcgat gacctggatg attttttgcg cataaccggg
    12241 gtctgtcgca taacctgcat cttgcaatgc ttgtgcggct tcctgatagt tggtggccgt
    12301 aatcactttt tcatacagcg caggattcca atctactcct tggacgaaca gttgtgtatg
    12361 gtcatccatg gactgttccc atgaatcata cactttgaag tctccttgga tagttatcca
    12421 ttcttcgtta acaaattcct ttgtttccag gcttactttc ttctgatccc cataagcctt
    12481 gatcccgaaa agattattgt atttgctagc caaggtactt tttccccaat ttgattctaa
    12541 tatagcttgt cctaaaatga tactaggaag cactccataa ccatcttgta gttcttgggc
    12601 atgcggaaca agccgttcaa taaattgctg atgggattgc gcttcatttt cttgctgatt
    12661 atcagtaaaa ggagaagaca gattcttcaa ggaaaaaaca aaggctaaga gtatcactgc
    12721 tacaccggca accactgctg gccaacggat attcttgcgc ttttgctttt tccacttttt
    12781 tttcgccatg atcaattcct acttttcatt taattcattc agatattctt ctagcgtcaa
    12841 ttggtgtttg gtgatatact cagcgttttc tttaccgaca taacgtaagt gccaaggttc
    12901 atatgtgatc ttagttatat cttcgtgtcc ttttggataa cgaacgataa atccatactg
    12961 cgcagcatta tctgcgatcc atttagctcc aggctgatct ccgtaacttg catccaatac
    13021 agtagaagga taactgttgt accagttttg atcgacgaca tcgactgcca gacctgtgtg
    13081 gtgttcgctg tatcctggct ccgtaatcgt ttctttagta atctttgtcg cctcctcttc
    13141 tgatacacct tgacgactca tcacatcttg gacattttgt tcgaaaactt gttgttggga
    13201 tgatacagac cggtaagctg agaccatcac taatggaaat ccagctgctt cagcagcctt
    13261 ggcaaatgct tcatattctg gagcaatgcg tttgtcgatc aagtatccgt tacttaatga
    13321 tgccaattga gtagactcat caatttcttt ttctaatttg tgatctggac caaccaaaac
    13381 tagcttccaa tcgtctgttt taacatctgg caattgtttg gacgtacgat cttttgtttc
    13441 tttgtccttt accttgctgc tggttgtttg actgttcttt tctttactag cagttgtttt
    13501 ggtgcttgtt cgttcgactg cttgattgcc ggcttgaaca tgcgaatcat catcgctaaa
    13561 aataacatag cccattgctg cgatcataat cacaactgct ccaaatccta aaaatttatt
    13621 cacttgaaaa atcctttcgt tgttcttcac gttccccatt cgcctcatga acgtaagttt
    13681 cttccagatt ttcattcacc caatcgagaa gttcgatttt ttctccttcg atctgttttt
    13741 gaacatctgc tggcagacga aaattcaaaa tatcatcata gccccattca tcatattgaa
    13801 tacttacttt caagtcaagg tatctaccag cctgctgatt atttttcaaa gggatcagct
    13861 cgacttttgc tgctttgctg ctaagccatt tttgcgcgat gcgtgttttg atttgcttgt
    13921 actctgcaac ggcaacgttt cgtttttcgg gaaaaataat cgtttgacgc aaaatctttt
    13981 gcgttagcat ccattcataa tcagtgaaaa gattttttac tttctgcatc gcttcagatg
    14041 gcagattttt ctcgccatct ttccattttt gccattgttc tggactgatc agcaagtatt
    14101 catctaaaaa atttttttca gtttcaaact gttctaataa tacactcata ataagatcaa
    14161 tatatacttg atgcacgttt atcccctcct tttaactatt ctacttaagt tttgatcaaa
    14221 aagcacctct taaatcgctg ctttcatttt ttttaatgtt tcaagtcctc tcctctttat
    14281 tcttcgtgat ttttgttata ataatagaca gacaagaaac tcaatccaac tatgaaggag
    14341 cgttttcaga tgaaaaaata tcaatgggga atcgttggtc ttggtaccat cgcacatgag
    14401 tttgccgaaa gcttcaatca agagacgagc gaactaacag cagttgcttc cagaacttca
    14461 gaaaaagcgg aaaacttcgc tcaccgttac aatattccaa aagcttacgg aagctatcaa
    14521 gaaatgttag acgatgctga aatcgacatc gtgtacattg ctgtaccaaa tagacagcat
    14581 atcgaccata tcttggcagc attggaagca ggcaaacacg ttctatgtga aaaagccatc
    14641 acgatgaata aaaaagaact ggctgacgct atgcgcttag cagaagaaaa gaatttgatc
    14701 ttagcagaag cgatgacaat cttcaacatg ccactttatc agcagttacg ctcaataatg
    14761 gatacaggaa aactgggggc tttaaaaatg atccaagcac ctttcggcag ttacaaagaa
    14821 ccagacccta aaaaccggtt cttcaatcca gaattagctg gaggagctct gttggatatc
    14881 ggtacgtatg ctgtatcttt cgctcgtttc tttttgagct ctcaacctga agtagttgcc
    14941 tctacaatgg ttccttttga aacaggcgtg gacgaacaat ccgtcactat cttgcgtaac
    15001 aaagaaaatg aacttgctgc tgtcagcttg acttttcagg caaaaatgcc aaaagtcggt
    15061 atcgttgcct ttgagaacgg ttatgtgacg attgctgatt acccgcgtgc tgatcgtgcg
    15121 gagatcttat ttacagatgg tacaaaggaa ttcatcgaaa gcgggaatac atctgaagca
    15181 atgaattacg aaataaagaa tatggtacac atgattgaag gtgacttgcc caatcgctct
    15241 ctcttcttga caaaagatgt catcgaaatc ttggatcaaa tgcaagttct atggaaaaat
    15301 atgtaatttt aaaaggttgt gaaaaagctg ttctgcatca agtaataaga acagccaaac
    15361 aaaaatagct cctcctattt ttcgtttggc tggacttatt accgcagagg aaagcttttg
    15421 aacaccgttt aagaaagagg ttgtgagcct accgttcagc tccgagtcac aaggagcaat
    15481 ttcaaaaaac agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttgacag
    15541 gagaacaccg tttaaagaag aggttgtgaa atcagctttt tgacctgaat attagctgaa
    15601 ttcacaacct ctatttttgg attttttgtt gggagaattc tgccttgatt tcaataaaac
    15661 ttttaaaaga ctgcaaaaac tggaacaatt ctgctcgatc ctcgaattca gggcgactag
    15721 tcggcagagg catttgccga tacaatacat aaacttcttc aatacgtgtt aaaatttgtc
    15781 tgccatcatt tgactctgaa aatgtttctg cagtataaat caataaacca tagatatgtt
    15841 ttccataata agctggttgc tggattctct ctagattttc cagcatgtct ttaatcacat
    15901 tggcctgtgc tcttctcata gagaagtaag tttcgtagta tagcggctgt ctgagccagt
    15961 agttttcttg atgttctctg gcatcttctt ggctttcgcg aatgaatgtc agtaaatgtt
    16021 cgcatttgat ctgcacatct tctttatttt ctgataaaag aaattctgcc atttgtctca
    16081 aaatatttcg aaattctttt tcgatctgta tttggttttc cttcagctgc ttcgtacgat
    16141 ctggcatgta aacatttgca agcaaagcaa atactaatcc aatcgccatt aatcccgctt
    16201 cattgccgat cagctgccaa gacatgtttt cctccactaa ataatgagtg accaaaaccg
    16261 aatttactac tatcccttcg gttaatttga agcgagaggc aagcgggatg aacaggaata
    16321 aaaaaagact gaagccaaaa atagtatacc caacagcaaa aaaactgaac agtgctgttg
    16381 ccgtagctaa tataaaggct gcgattcgat tcatgcccgt acgcaatgta gagcgcttgg
    16441 tatttcctac acttaataat gcaatgatcc ctgcagctgg ccaatacaac agcgataagc
    16501 tacccgctgt gaccatcgaa aagaagacac tgatcactgt tttgactgtt cgcattccta
    16561 tacgcattta cttccccctt ttttatttta attatacttg aataatgaaa aactagctat
    16621 cagacaaaag tttaggtatg atagtaaaaa gaccaaaaag gagaatctct atgcaatttg
    16681 gccgttttcg actaggtatg cgtacgatca aaagtgcctt agctgttttt ttatgtatct
    16741 tgttttttca tttgactcaa cgaggaatcc cgatgattgc tgcattatct gctgtttttt
    16801 ctttgcgtca ggacttgact acaaccgttt cttttggaaa gtctaggatc atcggcaata
    16861 cattaggtgg tacattagct atcatttatt tttttgtcaa aagctatttt cacaatgatt
    16921 ttttggtaga attgatcttg cttcctattc tagttattat tgtgatcgtc gtgtcagatg
    16981 gtattaacaa taattccggt atcatttccg caatagctac gttgttgctg attgcgttga
    17041 gcatccctca aggcgaatcc gctttgtatg ctatccaacg tgtactagat acttttatag
    17101 ggacattcat tgcaatcggt atcaatttct ttcttcgtcc tcctgaaaca gaaaagaagg
    17161 aagaactatc agaagatttg gtcgaactcc aaaagaaaga acgattgcta agaacaaatt
    17221 tacaagagat agaagctaag atccaaaaac aaaagaaaaa tagcgaggac taattttctt
    17281 ttgcaataaa ataaaaaaat ggagagaaga actttaaaaa gtcttcctcc atttttgatt
    17341 gaacggtgaa gtgtactact ttattcttcc ttcatcgagt tttgttctgc ctcttttggc
    17401 gcttcataca tatttacttg gctagtagtc ccacgtgtcg acaaattctc tacgatttcc
    17461 ttcaagtcaa gtcctgttgt cgcttttagt gtttcttgag tagaagaaag caaatttgtg
    17521 gcataatttg tcactcggtt cgctccactg gtttctcctg aactgcttcc tgtatcgaca
    17581 acagagattt tttcgatatt tcctagcggc tgtgctgctt cgcgcatcaa ttgcggtagc
    17641 atatcgataa ccatactcaa gacagctgct tcgccatatt ccttgaaggc attggcgatc
    17701 ttttgttttg cttcggcttc tgcttttcct tttgctagtg ctgcttcggc ttctgcttgt
    17761 ccagctaaac gtgtcttatt ggcttccgct tctgccagtg cttccacacg gaatcgttct
    17821 gcttcggctt cagtcacttc tttgactttt tgagcttgtg cttcttgttc tttagcgtac
    17881 cgatcggcat ctgctttttt cttcacttcc gaatcatatt gcttttcacg acgtgtgatt
    17941 tctttttctt ccagttcgat ttgtttttga cgttctacga ttttaatctg catttcctgt
    18001 tcgattactt gttgttgtgc tcgggcactt tctaaattgt aggcttggtc tgcttttgct
    18061 ttagcaacat cttgttcttg tttgtaggtt gctaatttca gttctttttc ttttaatgat
    18121 tctgcgatct ctgtttgtcg ctgtaattca gctgcttgtg attctttttc tgcttgagct
    18181 tttttgatac gcgtttcttt tagagcttcc gcttctgcta tatctgcatc tcttttgact
    18241 tgggcgattc ttggtttccc taaagagtca agataaccgt ttttgtctct gacctctttg
    18301 atagtgaaag aaacgatgat caaccccatt tttgcaaggt ctacagaagc gacttcttgt
    18361 acgctttgac tgaacttatc gcgattttga tagatttctt ctacagtcat agaaccaaga
    18421 atcgagcgca agtgtccttc taatacttct cttgcttcat tttccagttc ttctcttgtc
    18481 ttgcctaaaa actgttctgc tgctgtagca atctcttcta cagatgaacc gattttgatg
    18541 atcgaagtac catcacacat caccggtacg ccttgttccg tatacacttc aggggtggag
    18601 acgtccaatt tactcgataa tagactgatc cgattcgaac gttgaaaaac tggcaagacg
    18661 aatgcgccac ctccacgcac aattttgatt ttgttattgc tttcatcaat gtgcacattt
    18721 ttgcttccta aataacttcc actgatgatc aacgcttcat ccggttttgc tgtctggtac
    18781 ttcgctacaa agacgatcag caacatcagt aaaatcaaag ctgcgattgc gatcgcgata
    18841 aaaacaactg gtatttccat ttaacacacg ctcctttaag cttttatcat gctttcataa
    18901 ggcatgacat aacagattct ttccttgatc tcaatgatca atactttcgt tccccgctca
    18961 gctgtctgtt cttgggggct gtaaaaacaa gctggacgac taatggaacc ggtaacactt
    19021 ttcaactgaa tttctcccat acctcgtaca gggatcggtg tgatcacaac agctacttgt
    19081 ccttccagag ttttttcaga agacgacatc gttgattcgg catgtttcat cggcatcaat
    19141 atatagaaat taagcaaaaa taccaacact atcgatatgc ctccactagc aataaataaa
    19201 aataaagatg attgatctgt taaacgctca cctagaaaac caaataagct tgtgaaagtt
    19261 aaccaaggga taataagcat cggatcaagc gggccatcaa aatcgaatac atctccaaaa
    19321 ataatcaata ggactgccaa tcctgcacaa atcagcaatg tataccaata aatcgtctca
    19381 agtgcgattc cccctatcat atacacgcct ccttccattt ctacgtctat cataccaatg
    19441 tttgaaaatt ctgtatagct tcttttaccg actcagtggt gttttttata ggaaggttgt
    19501 gtttatcgtt gacaatttgt taaatttttg ttttcatgtt aaaattttca caataaattc
    19561 tttcgaggta gcctatgttt ccttttttta agaaaaaacc attaaagctc cagaacacga
    19621 tcatccttgt cgtttgtttc tccacattcg tttctttgat tgtttctgcc atattgatca
    19681 ggaattttat tctttcgatg aaccgatcta gtgtgtcaga taaaatctct agcattgcta
    19741 aaatcgttgc caatgataaa ctcatccaaa aaaagctgca gactggtgac ccgacacatg
    19801 cgattcaagg aataacaaat gaaatcatga ttgcatcgaa tttagatttt atcgttgtca
    19861 tggaccctga aggcattcga tattctcacg cagacccaag ttacatcggt cttcctttct
    19921 caagtcatga agatgtgaaa aaagtgattg gaggacaaga atttttctct gaaaaagaag
    19981 gaatgttagg gttcggcttc cgctatttcg tccctattta ttatgatcaa gagattattg
    20041 gtgcagtttg tgttggctta acaaacaaca cgattgacaa tcaagtcaaa gatggacaaa
    20101 aaatgattgt tttttcattg attattggac ttagcgtagg catcataggc gccgtttttc
    20161 ttgcccaaaa aatgaaaaaa gtactgcttg gacttgaacc acaagaaata gcagaacagt
    20221 taagtgaaaa aacgattatc gaaaatgaag tagatgaagg aatcttagcc atctcttctg
    20281 ataaaactat cctcttgatc aatacacctg caaaaaacat tttctaccaa acagagcagc
    20341 tattggattt cgaagtagga aaaaaatcag tgataatcta tatgacgttt tgtttaaaga
    20401 gacgattgag catcaaacga ccgttcggac tcaatccatg tatttaaatg ggatagaaac
    20461 tatttctagc gttacacctc tttttagcaa tgaccgtttt ttaggagcag tcgcaacttt
    20521 acgaaatcga tcagaagtaa tgaaattaat ggaggagctt ggggggacga aacaatatat
    20581 aaactccatg agagctcaaa cccatgaatt catgaataaa cttcatgtca tttctggttt
    20641 gattgatttg aagaagtatg aagaagtcac ccattttatc cagcagctaa atcaaaatta
    20701 taaaaaagag attggccaag tctcaagtct gatcaaagta cctgcaattg ccggtttttt
    20761 attaggcaaa ataaacgaag ccaaagaaca atctgttgaa atgctgttag aaagcgattc
    20821 ttttatccct aaaatggaga tggatgactc catccattca cttttgcaag tgttagggaa
    20881 cttacttgat aatgcaaaag aagccgtgct acaaaaacat gaaagaaacg gacaggtccg
    20941 gattcattta aactatgaca cagaagctga agtattcatc gtgaccgtct gcgatgacgg
    21001 tgtcgggata tcagaagaaa tctcagaaaa aatatttacg ctaggttttt cgacgaaggg
    21061 aatcaatcgt gggtttggat tgaatctcat ccattcaatc gtcaaacgcc acggtggatt
    21121 tttagaattt tccaaacaaa caaatggcgg tacaatcgtc tatctagaat taccaaaaaa
    21181 ggaagatcaa aatgatgaca aatgtcctga taatcgaaga tgatccaatg gttgcagaat
    21241 tgaatcgtac ttttgtggaa agcatcccta atatgaacgt aatcgaaagt ttcagaaatg
    21301 tagaagaagg aaaaaaatat ttcagacaaa aaaacgcaga tctgctgctg cttgatgtat
    21361 acatgcctgg cgaaagtggt atccattttt tatcttggct tagacagcaa ggaaatgata
    21421 tcccagtgat tttgatcact gctgcagatg atatcgaaac cataaagaat gtgatccgct
    21481 acggtgcctt agattatttg atcaaaccgt tcactttgca acgttttcag ctggcgatcg
    21541 aaaagtatct tgctttctct cagacaatcc aaacgactga aaaagccgat cagaaattga
    21601 tcgatcaagt attcaaccac accactccca ttcacgaaat accatcattc atcgatttgc
    21661 ctaaaggtct gtcaaaactg actttaaaaa aaataagtgc atttattcta aaagaagata
    21721 ctattttttc aacaaaagaa ctggctcaaa aaagctcact gtcacgtata tccgtcaaga
    21781 aatatttgaa ttttttagta gaacaagata tattgaacga aagaatcaaa tatttggaaa
    21841 tcggccggcc gctaacctgt tatgaattga caaagaatgg gcggaaaaag ctcacagatt
    21901 actgttgagt catcgaatta tcccatacaa aatagtccaa gtatacagaa gtaacaactg
    21961 tttacttgga ctatttttaa acattttatt gacgaatatc catgttttct tatcttaatt
    22021 gacgggcttt aggaagagat acctcatttt tcgtttttcc agtttctatt atttcacgaa
    22081 agttctcgaa acttgtcgaa atcatatttg ataatgcctc atctgtattt gaacctacat
    22141 gcggggttac aagaactcta gggtacattt ctactaattg ctggatagca gggtctggaa
    22201 tcgtctccca tggcttgaag gatcggaaaa aaacctctgt ttcatttgca aaaacatcgg
    22261 ttgcaaaacc agctaaatga ttgcttttca aagctcttaa aatagcttca ttatcttgaa
    22321 gctctccacg ggctgtatta atcaagatgc tacctttttt catttgagct aaaaatcctt
    22381 cattgatcat ttgatcgttt tcacctggga tatgaggtac atgaatactg acaatatcgc
    22441 ttttggctaa taactcttca agcgagtgga acgtcaatac ttttttagca gcttctgtct
    22501 gatgaatatc gtagccgatc actttggctc ctagtccctt aaacaaaaca gcttccgtca
    22561 accctatcct gcctgttcct atgatcccta ctgtgcaatt tctgatttca cgactaaaca
    22621 tttgctcatc cactcgaaaa tctttgaaag atgttcggat cgtcgtatac gcagtatttc
    22681 tcaaaagcat catggcaaaa gtcaaagata actctgctat cgcatttggc gaatacgatg
    22741 gtacacgggc tactgtcata ccaaaatcag ctgccgcttg tagatcgata tgattgactc
    22801 ccacagtccg tgtaaaaaca taacgaatcc catattcctg catttttgca agattggtct
    22861 catctgctat acaatttccc ctcaacaata cagcatcatg gtcctttgct gacacgatat
    22921 tgtcatgtgt caacagttct tcataaagac tgagatcata atggtaaata ttcaattttt
    22981 caaaaaaatc tatctcgttc ggacgaacac cgtaacatgc gattctcata ctttcttcct
    23041 ccatttctta tgaaaataaa cctatgccat gaatcatttg taatacaacg atccatacag
    23101 gtgtcagtac cacagcaaaa atagttgaca gtaaggatgc attggaggct aaaagtgcct
    23161 ccctatcaaa actgatagca taggctgctg ccactgttgc agctggtgta gccatcatga
    23221 taacgattgt tgccagtgct tcaaaattga ctggtagaat attcgttgcc gtcaaaactg
    23281 ctagaatcac gatattcaat gcaggtacaa ataccacttt gactagactg taataccatg
    23341 aagttttttc agatgcagct tttttgaagc ttacttcacc taatgttgcg ccgattgcta
    23401 gccacgccaa tggtgaactt aaaccagcta aataatccat cggctgaaat agccacggtg
    23461 cagtctggtc aattcgcaaa aacgctactt ggcttatctc tccttctgca cctgtaactg
    23521 acacttgtgg cagataccct tggaacagcc aaatgaaaag gccggcaaaa gtagcgataa
    23581 tgattggatt gacgaacatc gctttgatat ttttagcttc catcttcaag ccactcattt
    23641 ttatgtatcc ataagaatac aaaaagattc ggtagccaat attgaaaatt gaggaataca
    23701 tcacgccgac aggaccataa atcgcactga cgataggcgt tccgaaaaag gtcgtagaac
    23761 caaagatcgt caggacacgc aacgttgttt gcttatcccc tttatagctt aagaaaaaag
    23821 gttttgaaac tagaataaga ataatataaa taactaatcc ccaaatcaag acattgattc
    23881 cttgcatcag taattctgta ttgatatctt gcatgaaagc tcgaaatgcc agtgctggca
    23941 aagccactgt taagacgact ttcgatagaa tttttccaac agattcagaa aaaatcccct
    24001 tttttctaca aacaaaccct aataatataa taaaaactgt tgaagtgatt gcactgacaa
    24061 tcgacatatc tgtcaaagtc gatcgaaaaa tgtcaaataa gttcatcttt ctcctcctaa
    24121 ttatgttcat ttattcacaa taaaaaaagc ttttattttg ctaatacgta ttaacatttt
    24181 taattttatc tttacattgt tacttattga acaataggaa attaaagaaa cttttgaaac
    24241 ttatgtaaag tgatt
//
LOCUS       DAYYKN010000031        24046 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000031 DAYYKN010000000
VERSION     DAYYKN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.031"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1086
                     /locus_tag="ACHBB7_001048"
     CDS             <1..1086
                     /locus_tag="ACHBB7_001048"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9209014.1"
                     /translation="ILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIK
                     GYGYVVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVA
                     HQYQVFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSL
                     EKDQEKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLK
                     NIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEAS
                     IESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEE
                     INKKNPNMFVNYQNDPYQFTTQVILLF"
     gene            1101..1853
                     /locus_tag="ACHBB7_001049"
     CDS             1101..1853
                     /locus_tag="ACHBB7_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9209015.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2556..2771)
                     /locus_tag="ACHBB7_001050"
     CDS             complement(2556..2771)
                     /locus_tag="ACHBB7_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFM9209016.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2790..3026)
                     /locus_tag="ACHBB7_001051"
     CDS             complement(2790..3026)
                     /locus_tag="ACHBB7_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFM9209017.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            3284..5437
                     /locus_tag="ACHBB7_001052"
     CDS             3284..5437
                     /locus_tag="ACHBB7_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFM9209018.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5406..6821
                     /locus_tag="ACHBB7_001053"
     CDS             5406..6821
                     /locus_tag="ACHBB7_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFM9209019.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6854..7888
                     /gene="sppA"
                     /locus_tag="ACHBB7_001054"
     CDS             6854..7888
                     /gene="sppA"
                     /locus_tag="ACHBB7_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM9209020.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7890..8411
                     /locus_tag="ACHBB7_001055"
     CDS             7890..8411
                     /locus_tag="ACHBB7_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM9209021.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            8408..8794
                     /locus_tag="ACHBB7_001056"
     CDS             8408..8794
                     /locus_tag="ACHBB7_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM9209022.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(9290..9362)
                     /locus_tag="ACHBB7_001057"
     tRNA            complement(9290..9362)
                     /locus_tag="ACHBB7_001057"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9327..9329),aa:Lys,seq:ctt)
     gene            9743..10312
                     /locus_tag="ACHBB7_001058"
     CDS             9743..10312
                     /locus_tag="ACHBB7_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM9209023.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            10299..11159
                     /locus_tag="ACHBB7_001059"
     CDS             10299..11159
                     /locus_tag="ACHBB7_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9209024.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            11349..12053
                     /gene="yycF"
                     /locus_tag="ACHBB7_001060"
     CDS             11349..12053
                     /gene="yycF"
                     /locus_tag="ACHBB7_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM9209025.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            12058..13893
                     /gene="walK"
                     /locus_tag="ACHBB7_001061"
     CDS             12058..13893
                     /gene="walK"
                     /locus_tag="ACHBB7_001061"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM9209026.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            13890..15206
                     /gene="yycH"
                     /locus_tag="ACHBB7_001062"
     CDS             13890..15206
                     /gene="yycH"
                     /locus_tag="ACHBB7_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM9209027.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            15207..16076
                     /locus_tag="ACHBB7_001063"
     CDS             15207..16076
                     /locus_tag="ACHBB7_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM9209028.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            16131..16940
                     /locus_tag="ACHBB7_001064"
     CDS             16131..16940
                     /locus_tag="ACHBB7_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9209029.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(17043..17672)
                     /locus_tag="ACHBB7_001065"
     CDS             complement(17043..17672)
                     /locus_tag="ACHBB7_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9209030.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            17842..19134
                     /locus_tag="ACHBB7_001066"
     CDS             17842..19134
                     /locus_tag="ACHBB7_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9209031.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(19210..19872)
                     /locus_tag="ACHBB7_001067"
     CDS             complement(19210..19872)
                     /locus_tag="ACHBB7_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9209032.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            20097..20381
                     /gene="groES"
                     /locus_tag="ACHBB7_001068"
     CDS             20097..20381
                     /gene="groES"
                     /locus_tag="ACHBB7_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM9209033.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            20432..22057
                     /gene="groL"
                     /locus_tag="ACHBB7_001069"
     CDS             20432..22057
                     /gene="groL"
                     /locus_tag="ACHBB7_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM9209034.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(22146..23285)
                     /locus_tag="ACHBB7_001070"
     CDS             complement(22146..23285)
                     /locus_tag="ACHBB7_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9209035.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALENYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKMIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYGEGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLSNRAKELIESVIA
                     DNILAGRPTNPDQYIFISTNGTPLTLHSFNAVLHKVEEELELEKNLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKKAKQQVIDKLNRL"
     gene            complement(23396..23980)
                     /locus_tag="ACHBB7_001071"
     CDS             complement(23396..23980)
                     /locus_tag="ACHBB7_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM9209036.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
BASE COUNT         8090 a         3857 c         4730 g         7369 t
ORIGIN      
        1 atattaaagg aaaagaagaa aactaatata gtgcttttaa ataaattact attatgtatg
       61 cttattgatg atttcagatc tattattact tccatggtta tgacgtatat gttttcattt
      121 gaagggataa aagggtatgg ttatgtagta ttccagataa tgcttgattc tttgtttttg
      181 ctgatgttta tttggttata tagaaaatta gctataaata ggattgttga acagtccagt
      241 tctaaactta ctgctatatt catgatttat ttacttacaa ttggtttatt tgtttcttat
      301 gttgctcatc aatatcaagt atttgatcat tttgtgtttg gtgtcttaat atttttaatc
      361 attcaaatgt ttgttgttct atttttattt attagaataa ctattaggca acgagaacaa
      421 tatgaacagc aattgaaaag aaaagaattg gtttatctaa aaaaatatac tgattcactg
      481 gaaaaagatc aagaaaaatt tgccagattt agacatgact ataaaaattt attgctgagt
      541 ttaaaagaaa tcgctaatca aaaccatgat tcgatattgg aaaaagaaat aaaagaatta
      601 gagatctatt ctaattctta tctaaccaat aagtttgaat atcgttattt aaaaaatata
      661 aaaaatgaat atttgaaaag tttgttgata gcgaagctat accaagcaaa taacaataag
      721 atttgttgta aattcgaatg tccaagtact attattgaag ttcctgttcc aatatttgac
      781 tgtattaggg tgcttggaat tattctcgat aatgcaattg aagcatctat agagagtcaa
      841 gatagagaac tgtctctagc tatttaccga gatgataaac agatagaaat ttcgatatcg
      901 aatagttgta gagagatact actctctata gatactctaa tgaaaaaagg aataacaaca
      961 aaaaagggac atcaagggtt aggtttatct agtatagagg aaatcaataa aaagaatccc
     1021 aatatgtttg tgaactatca aaacgatccg tatcaattta ctactcaagt tattttgttg
     1081 ttttgaggga aggaggattt atgagttatc caataatatt atgtgaagat caaattatac
     1141 aattaaatca gttagaacga attattgata atttcattct ttttcacgat aaagtattta
     1201 agatagtatt gaaaacacaa agtccgttag aagttaaaaa atacctcaaa caattccgac
     1261 caaagcaagg aatatatttc ttagatattg atttaaatca tgaagttaac ggtatagaat
     1321 tagcagaagt aatcagaaaa tatgatgttc aagcaaaaat catttttaca actactcatg
     1381 atgagatgtt acccgtaaca ataaaaagaa gagttgaaac gttaggattt gtaacaaaag
     1441 atcaaacact agatgagtat cgaaacgaga ttgttgagtt attgttatta gcgcaagaaa
     1501 ggatagatgc aacaaaacag gttacgaatc aagcatttgt tttttccata ggttctcaaa
     1561 catttacttt tgatataaat gacatttatt ttgtagaagc ttcaaaatta ccacatagat
     1621 tatcattgtg tactaaagat gggcagtacg aattttatgg aagaattagt gagcttgaaa
     1681 agaaatatcc tatgttaaca agaattagtc gagcgtgttt agtaaatatc tttaacgtta
     1741 aagaaataga ttttaaaaaa cgttcactat attttgattc agagttggcc agaaacttta
     1801 ctttagggaa agcacaaaaa attaaggaga agttgaaaga aagcactgtt tagtggtttt
     1861 ttctttaggg agattgcatt tttctaataa aatagattcc ttcactagtg caaatttttc
     1921 tgaatcctat taaagttgta ccaattttta tagctgttat tagttacgtg cataacagca
     1981 atattagtta ctggaggaat atttctatgg tctggaacca aaaaagggcc aaatgaatag
     2041 ttcttttctt gtttttatta agtaccataa aggggaatta aaattaaatg tatggtggaa
     2101 gatgcgaaag atttggcaaa ttctactaat ttttagaaag tgtttttcat tttttagtta
     2161 aaaaatttgt cctgtcaagt gcaatcctat gattgtactt gataggattt gagataatta
     2221 ctttcctgtt tttataaatt taacttgcag gaacaggctt taatgcacat ccgacaagta
     2281 cattaccaat acctcctgta acggccgaaa taggtcctcc tgcagcacca actacagcgc
     2341 tgcaagtcct tttgtaaaaa tatctctacc attacatttc caaagaattt ttatctgact
     2401 ttaataaaag ataaaaattt aatataatta taaaaactag cacaagaagc tttagtaaaa
     2461 agttctgtcc attccaaact tttgaaaaga taaagaacat acctatgttc gtaatcgaca
     2521 caaaccaatc ttgaggtttg atatttttta attgtttacg caccggtcca catgctgaat
     2581 ccagcaccta ataaagctcc acctacacca caagcagcta ttgcataagg tcctccggct
     2641 gaacaaagtt ttactcctgc tgttgcccca gaaaaagtag tgcctaaaac tcccacggca
     2701 ctcccacctt gaatattttt caactcttct gaagataata taaatgttgt ttttgtgttt
     2761 ttaatatgca tactacttat tctccttttt tatttttttc gagcatccca gccagctgca
     2821 gcacctccag cgacagctcc atataaccct ccaacaacac cgcaagctgg tccgcaaatt
     2881 gctgttccta aacctgcacc acctactgcc cctgtggtaa gtccacctac aacttgcccc
     2941 aattttcccc aagcattatt gcctccacca acagtttgtg catttccaca atacttagtt
     3001 cctttacatt ttgcatgttt aaacacgctt ctttaaataa tattttttac ggtgctactg
     3061 taacatacgt atttgattaa cagtaaaaat cttcctgaaa cttatcaagt ttattcttga
     3121 atagtaaaat ttccaaaata aatttgattc aggaatgaaa aagttagtgt tcaagaactg
     3181 ttttcaaagt ttttgtggaa ttatgagaca atattttcac tgtattaaag ctatattgag
     3241 aatttttcta taaaaatttt ttagaaggag ttgtgttaga taagtgataa aatcatttcc
     3301 ttttgtgcag caacaagatg aaaaagattg tggtgttgct tgcctttcga tgattttaaa
     3361 atattttaaa actgatatcc cgattcataa attacgggac ctatctggga ctgatttaga
     3421 gggtacttca gcatttggat tgaaaaagac atttgaaaaa ttgtcttttg attgtatggc
     3481 tattcaagca gatagtgaga tctggaaaga aagagatctt atcttaccat taattgcaca
     3541 tatattggtt gataacagct atatgcacta cgttgtaatt tataaaacga agggaaacgt
     3601 actatggatt gcagatcccg ctaatggaaa aaatagaaaa acgattgaag cgtttcaaaa
     3661 agaatggaca ggtattcttt tattacctat gcctagagaa acttatattc ctattaaaga
     3721 aaaagtagct ggattaagtt cttttttgcc tataatttgg aaacaaaaag gattagtatt
     3781 ccacatcgtt ttagcatcga ttttcattac gttttttggc ataggaagct catattattt
     3841 tcaaggtatt cttgattttt tcatccctaa tcaagcacgt tcaactttaa atataatttc
     3901 cattggcttg atcgctgtct atttatttcg ggtattgttt gaatatagtc gtagttatct
     3961 tcttattatt ttaggacagc gaatgagtat ggcaattatg cttcagtatt tcaaacatgt
     4021 gttaacttta ccaatgaatt tttttgctac gagaaaatca ggggaaatta tttctcgttt
     4081 tcttgatgcc agtaagatta ttgatgcttt agcaagtgct acgttatcaa tatttttaga
     4141 tattggtatg gtgttgcttg ttggggttac gttagctatt cagaatagta cgttgttttt
     4201 aatcactcta tgctctttgc ctttttatgt agttgcaata ttcgctttcg tgaaaagcta
     4261 tgaaaaatcg aacagggaag aaatggcagc cggtgcagct gtaaattcta gtatcataga
     4321 aagtcttaaa ggaatagaaa caattaaagc atatagcggt gaagagaaag tatataaccg
     4381 tatagaccat gaatttgtta aattaatgaa aaaatcattt cgtacggtta cgcttgataa
     4441 tattcaacaa ggaattaaac atttgattca attaattagc agtgcattaa ttttatggct
     4501 aggttcttat tttgttatgg ataatagaat tagtctaggt caacttatca catacaatgc
     4561 tttactagta tttttgacag atcctttaca aaacattatt aatttacaag tgaaaatgca
     4621 aacggctcaa gttgcaaata atcgtttaaa tgaaattttc gcgattgatt cagaaaaatc
     4681 gaaccagtct tttgaatata tgctttctga acaattattt caacaagata tcactgtaaa
     4741 aaacgtttca ttctcgtata gcatgaaggc cccaacgttg aacgaaattt catgtacaat
     4801 tccttcgcac agtaaaacgg ctatagttgg agttagtggt tctggaaaat caactcttgc
     4861 aaagttattt gttcgttttt atgaagcatc agaaggatcg atccattatg gaaacattaa
     4921 tcacttagac attccttttc agatattacg cgatcgagtg acatatttgc ctcaagagtc
     4981 atttttcttt agtggaacaa tcattgagaa tcttatattc ggtttagata gcccaccaag
     5041 tttcgaacaa ataatagaag tttgtgagat agtccaacta aatgaattta ttaatcaaca
     5101 gccattacgt tacgagacat tgttggaaga aggtggaaat aatctttcag gagggcaacg
     5161 acaacgatta gccttagcac gggctttatt aaaaaatccg gacatattaa ttttagatga
     5221 agcaactagt ggattagata ccttgttaga acatgcaatt atgaattatc tcctcaattt
     5281 aaaagaaaaa acgattattt ttattgcgca tcatttacca atagcaaaag catgtgatca
     5341 aattttagtt ctccatgaag ggcatttggt agaaaaagga acgcacgatg aacttcgttt
     5401 tgccgatggt atctatcaaa aattatggga aatctaggag agggaaacaa tttatgcaga
     5461 ataataattg gttcgaacat tctagtatct atactaaaca acatcatagc ttctaccgtt
     5521 gggtgttata ccctgtaatt ctttttttat gtattattgg attgttttta acctttgcta
     5581 aaaaagaggt tgttattcgg acaacggctc aattgaaccc cgaaaaaata gagaagttac
     5641 aagtgccatt agaagctaaa ataatggaaa acttcctaaa agaaaatcaa tttgttcata
     5701 aggacgatgt gttggttaga ttggattgtt ctttaataga gaatgaaaag gctcagatag
     5761 aacaagaaaa ccaacgtatt actcaacaaa taaagatggc tcagctattt attgaaagta
     5821 taagtaaagg aaaaaatttg ttttcaacgg atgacagttt tggctacagt aatcaattaa
     5881 agagcatgtt gtcagaaaaa gaatcactcc gctacgcttt gaagcaaagt gaattaaatg
     5941 atcaaaagca attagaagta tacgaaaaga caaaaagaca actagaaaaa caaattgaga
     6001 gttcagatag taaattacaa gaatggcaac aagtacaggt agcttggagt aataatcaat
     6061 cattaaaaga tttttcaaaa gaaatgatgg caaactatga gaattggcaa gaacaactaa
     6121 ataatgtttc tgatgatcaa aaaaatcaag tgaaactgac aatttcagca agcataaatg
     6181 aacaaattga gcaactaaaa aaagaagtag aacagtatca gtcagaaaaa gctaaattag
     6241 ttaaaccaac tacttctgag aatgacagaa ttagtcaaac ggaaaaagga aagcaagagc
     6301 tagaacaagc aataacaact gtaaaacaaa cagttacaga gctaaatgaa aaacaagaga
     6361 agaaccagtc agttatcaaa agtttggacg atcaactttc aaaaggaata ctaaaagctc
     6421 ctgtatctgg aacggttcat ttaaatgaag aaacaaaggg acaagcagaa ctagccaaag
     6481 gaacagtgct tgcagaaatt tatccgatcc acgaaaaatc aaaaatgaaa tttacagcac
     6541 ttttacctgc gaatgaaagt acctatatta aagaagggat gaaggttcat tttaaattgg
     6601 atcaaaaagg tggggctccg attacgatcg acggaacttt agatgaaatt tcagaaaatt
     6661 ctactgccac tgagaaaggt gttttttatg cagtgaaagg aacgttaaaa acaactcaat
     6721 cgtttccgtt tcgttatgga ttaactggag agttgtcttt aattgtagga gaaaaaacat
     6781 attggcaaca aattgtcgat gtgtttctaa ataaaaaata attattttta ctaagaatat
     6841 aggaggagtt tatatgaata aaagaagatg gattgctgtg ggagtagcgt gcggattatt
     6901 gttactatcc attatcgtcg cgctaattcc aggaaaagat aaagaggaag catcaaatac
     6961 aaccctaaca ggaataaata agatttttta cggatctaac gaaatcacag aagaaacgtt
     7021 agaagaaggc gcatcaaata aaaagattgt caaactatcc gtcaatggag ttattgcaga
     7081 tacaggtgaa agcaatcttt ttagtaggga acaatataac catcagaatt ttttaacaca
     7141 gctaaagaaa attcaagaag acaaagcggt taagggagtg ttattagaag taaattcacc
     7201 tgggggagga atatatgaaa gtgctgagat tgctaaagaa atggcaaaaa ttaaaaagct
     7261 agatattcct atctatactg catttaaaaa tacagcggca agtggagggt actacatttc
     7321 tgctggttca gataaaatat ttgctacgga agaaacaaca actggatcaa ttggtgtcat
     7381 tatttctggt ttaaattatt ctggcttatt agaaaaactt ggcgtaactg atgcgacgta
     7441 taaaagtggt gcattaaaag atatgatgtc acctcaacac aaaccttcag aggaagagaa
     7501 taaagttata caggaatttg tgatgagtgc ttatgatcgt tttgtcaatg ttgtagcaaa
     7561 aggaagaaat atggatacta atgcagtgaa agaacttgcg gatggaagaa tttatgatgg
     7621 taaccaagca gtagaaaatg ggttggtgga tcaaattggt tactctgaag atgcattaga
     7681 ttccctaaaa aaagagaaga aattaactga tgcgacggtt atagaatata aaaatgacac
     7741 aactggcttt gcttcttctt ggttaggtaa taagattgct gaatggcaag ggctaaaggc
     7801 aactacatca gatcgtgtaa tttcagtttt tgaaaaactt ggattttctg atactccaaa
     7861 accaatgtat tactatggag ggctataaaa tgaataagaa gagtggcatt gaaactttgc
     7921 ctaattatgt atttgcggga ttttggttac gattttttgc ttttcttatt gatttaattt
     7981 gtattagcat catcacccat gctactatag gtctctttta caaaatcatg ggactggaat
     8041 ctgacaaatc cattattact ttttatggta tagcgtcatt agctatttat ttaacctatt
     8101 ttgtactttt gactaagctg aattcaggac aaacaattgg gaaaatgatt tttgggataa
     8161 gagtagtgag ctttgaggaa aaagagctaa gttggcaaac tatctttatt cgtgagtgta
     8221 tatgtaggtt tatattaaaa aattttgtat ttcaaattgg gtatttggtg gctgctttca
     8281 cgcccaagaa acaacatatt ggggattatt tttctaacac gtcagtggtt gttattcgtt
     8341 cattagaagc tattaactat ggtcaaaaaa tttctaattc taaaacactt aatctggaga
     8401 gaattaaatg aataagagaa aaacagagtt tattttgatt ttaattagtg gaataacagg
     8461 gattttatgt agtcttttag cattgatggg tggtatgttt ctatcttcta ttgattcaac
     8521 tactttaaat actacagaac taaccagtaa ggatatagat atgttagtca atctaagcgg
     8581 gaatgttgtt ttatttggag gaattaccct agttttttcg ataatgattt tcataatagg
     8641 ttttttagta agaaaaaatc aaaaaacagt ggtctatggt attagtttac tcattttatc
     8701 tattattcca ttttttcttt taacattatt gtgggtaatt cctggagttt taggtattat
     8761 tgcgggattg atgcttctaa taagaaaatc ttaattaggc aatgtgtaga caacgtgaaa
     8821 cgattaatca attaaaagct aaatacgttt tgagaaatga taacttgtag tcgatggaag
     8881 acatgtagat aaaaaaacta cgtgtctttt atttattcta attttttcga aaaaaagaca
     8941 ctctctgatt gttgtattaa tggaaaaggg gaaagcagca agtaaattta gtttttctat
     9001 taaatattct tcacttttta accttttata gtacttagat ttaatcctat atgtttagag
     9061 agttcattgg cagcatgatt ttgcaaagct tctcgaatta ttgttatatt agaaacttta
     9121 ctgttcataa ttatgcttct tttttaactc aaagtccaaa gggctacagt ggactttgag
     9181 ttaaaaataa aaagccctag ttattttgaa aaataaaaaa agaacagcgt aaatgttttc
     9241 gaaatgcttt cgcaccaaca tttacctgtt ccgtgggttt ggcacccaat gggccgtgag
     9301 gggctcgaac ccgcgacccg ctgattaaga gtcagctgct ctaccaactg agctaacggc
     9361 ccgtacaaaa agtacttaat aatagtagca ctaagaattt taaaatgcaa gactacattt
     9421 aaagaaaatc agaaaaatct ttacttcctt ataggaaatc attcatagat tattcacatt
     9481 tgctcgctat tatataagca taccaacaaa caaagttctt gcaagagaac aaaccctaac
     9541 acttttttca ttttttactc ctttcccgcc gtctccccgg cgggtctcct gttcttaacg
     9601 ataaaagaca agaaggctta gccttcttgt tttttgtttt catggaatcc cgctaatcaa
     9661 atcacctagt taaagcaact agattgaatg tttatcataa gtctgatatc atgtttatga
     9721 aatgaaaaaa ggagcggtta tgatggaaaa aatgcgtcag caattaatgg agtgggggaa
     9781 ggaactagaa acattccgtc tgcctcactg ggaagaactc cctgacttag aactttatat
     9841 ggaccaagtc agaacgttag ttgatcgtta tctttcacct gtgatacaag gagaaaagca
     9901 tccattgctt acctcttcta tggtaaacaa ttacgttaaa ctaggtttga taccagcacc
     9961 agttaaaaaa agatataaca aagaacacgt agcattttta cttgcaatca ctacgttgaa
    10021 acaagtattg actattcctg aaatcaaaga agggatattg ttccaaggaa aaacagtagg
    10081 aattcgtgaa gcctataatc tattttgcga tgaacaagaa gcagctgtgt ggatggtcag
    10141 ccaattagca caaggtaaat ctcatccgca aaaattcgac caggcaaccc ctgtagaata
    10201 tattgctgta agagcggcaa ctttatcgtt tgcaatgaaa cttcttgcgg aaaaaacgat
    10261 tgttctagaa accgaatatt taaaagagga gaaaacaaat gaaaaacaat aaaatcgcaa
    10321 ttttagtaga ttcaggtacg gatgttccag aagaattgat aaaaaaatac gagatgtata
    10381 tgatcccatt gaaaattatt tataaagatc aggtatatac agataaaatt gacattaccc
    10441 ctgaagagat ctatcaacgt ttgccagagg aaatccctgg tacatctttg ccggatggtg
    10501 agtcgatcac agaaatcttt gagcaaatca aaaaagacgg ttatgaaaag gtacttgcgg
    10561 taacaatttc cagtggattg agcgggacat ataatgtggt tcgtttaatt gcacaacagc
    10621 aagaagaatt ggaagcatat gtactagata cgaaaaatat tggtattggt gcaggttttt
    10681 ctgcgatcca agcagcaaaa tggctggaag aaggagtaga atgggatcaa ctgatcagta
    10741 atctgaatga gttagtcgaa agaacaaaag ttttctttaa tgttgcgaca ttggaatatc
    10801 ttcaaaaagg tggacggatc ggtttagttg cttctattgt aggaaccgct ttaaagctta
    10861 acccaatcat ctcatgtaat gaagaaggga tttattacac agtaggaaaa gctaggggac
    10921 gtaaaaaaag tttggataaa acggttgctt acgtcaaaga aaaaatcggt aatgcaaagg
    10981 tcttcaactt agctgttgcc catggggatg cgaaagaaga agcaattgaa atggaagcga
    11041 gacttaaaag agagttccca caagcaaacc aaatctactt tggccagatt tctccggcat
    11101 tagtagtcca cactggtccg ggattattgg gtgtcggtgt acaagtttta gaaggataaa
    11161 tcaaaaaaag gaaacactga ggtgaaaatc agtgttttcc ttttttattg aagttagaac
    11221 aaaaaataat aaagtatttt gcactttcat tttaattttc tcttttgttt tataggcaaa
    11281 gatgaaacaa aagcagaaat acggtataat gagaaggtat gacaataata tataagaggt
    11341 ggctctttat gaaaaaagtt ttagttgtcg atgatgagaa gccgatttcg gatatcgtca
    11401 aattcaattt agccaaagaa ggctatgacg tttatactgc atatgatggg gaagaagcct
    11461 tggaaaaagt tgctgaggta gaacctgatt tgattttact tgatttgatg ctacctaaaa
    11521 tggacggctt agaagttgct cgtgaggtac gcaaaactta tgatatgccg atcattatgg
    11581 taacagccaa agattctgaa attgataaag tcctaggctt agagttaggc gccgatgatt
    11641 acgtgaccaa gccattttcc aaccgagaat tagttgcacg tgtcaaagca aatcttcgtc
    11701 gcggtgccac ggctgcgaag gaaccagaag aggcagcacc tgcggaattg acgattggtg
    11761 atttgacgat ccatccagaa gcttacatgg tgacgaaacg aggagaaaca atcgagttaa
    11821 cacatcgtga attcgaacta ctcttttact tggcgaagca ccttggacaa gtgatgacaa
    11881 gagaacattt attacaaacg gtttggggtt atgattattt tggagatgta cgaacagtgg
    11941 atgtaactgt acgtcgtttg cgtgaaaaga tcgaagataa tccaagccat ccaaattatt
    12001 tagtgacacg tcgtggtgtc ggttactatt tgcgtaaccc agaacaggag taattggatg
    12061 aaaagaaaag tccgcttttt tcgatcagtc aattttaaga tcgctattac ctttattctg
    12121 attttattga tctcgatcga aattattggt gcctacttta tacgtggttt agaacgttcc
    12181 acgatcaata catttattaa agatatgaat caaacggtgg aatcattagc aacaacaatc
    12241 agcccggaat tgaatcgaaa agataatgca gatgatgaag aggtaaatgc gaatatcaaa
    12301 agatttattg aaaacagtgc gactagcgat atcatcgaaa tacgggtcgt tgacgaaaaa
    12361 gggatcatcc gaggaacaac cgatgtcaat gaacaaagcg ctgtgggaaa gaaaaatgat
    12421 tatgttgata tcaatgattt cacgactaaa cgttatgttg ctttagataa cgacgataaa
    12481 cgggtgaata tcaatgttca accgatcctg tccccgaccg gcgatgccgt tatcggggct
    12541 ctttacgtca aaagtaatat cgaacaaaaa tattcggaaa tcaataacac agcagtcatt
    12601 ttctttactg cctcactaat tgcggcattt atttcaatga tcgtatctgt tttagttgct
    12661 cgttcgatta cacagccgat cggtgaaatg agagaacaag ccattcgaat cgctagagga
    12721 gactacagtc ggaaagtcaa agtgcatgga caagatgaac ttggacagct ggctgatacc
    12781 ttcaatcagt tagctgaacg tattgaagaa gctcaagata caatggaagc tgaacgcaat
    12841 cgtttggata gcgtgctatc ccatatgaca gacggcgtca ttgcgacaga tcgcaggggt
    12901 aaagtcatta cgatcaatga tatggctgtt tccttgctag atatcaaaaa cgaggaagca
    12961 atcggacaat cgattttgac acttttagat atcgaagaag aatatacgtt gcggaaatta
    13021 ttagaagatc caaacgaaat ggtacttgac cgttcaacca gtacattgga aagtgatcag
    13081 atgatcttgc gagtagactt ttctatgatc cgtcgcgaat caggttttat cagcggatta
    13141 gtagctgtct tgcatgatgt gacagaacaa gaaaaaacag aacgggaacg tcgtgaattc
    13201 gtctctaatg tttcccatga acttcgtaca ccgttgacaa gtatgcgtag ttatatcgaa
    13261 gcattgagcg aaggtgcgtg gaaagacgag gaagtagcac caaatttttt gaaggtaacg
    13321 ctagatgaaa cagaccgaat gattcgaatg atcaatgacc tcttaaatct atctcgcatg
    13381 gatacaggta atacccagct tcaattagaa tatgtcaatt tcaacgagtt agtgaatttt
    13441 gttcttgacc ggtttgatat gatggtggga aatcaggaaa aaaattataa gatccgtcgt
    13501 gaatttacgc aaagagatct ttgggtcgaa gtagatacag ataaaatcat ccaagtagtc
    13561 gataatatca tgaataacgc gatcaagtat tcaccagatg ggggaacgat tacttgtcgc
    13621 ttattagaaa cgcataataa cgtgatttta agtatcaccg atcaaggatt ggggattcct
    13681 aaaaaagatc tgaaccgagt gttcgagcgg ttctatcgag tagataaagc acgtgcaaga
    13741 gcacaaggtg ggacaggact tggattagct atttctagag aagtgattaa ggcgcatcga
    13801 ggcgctattt gggcagagag caaggaagga aaagggtcga ctttttatat ctcgctgcca
    13861 tatgaacctt atgaggagga atggtgggaa tgagaatctc tgaaaaagtc gttcgtatag
    13921 gactcatttt gatggttgcg ttaagcatat acttctctta tgcaatctgg ctgagcccag
    13981 caggaaaaag ttcaatcaac gtagatgaga caaacactca aatgatcgag tctcaaagca
    14041 atcgaaaaac ttccgaagtc tttcttccgc ttcatgttac atggcaccat tcgggaatga
    14101 tccaagaaac caacagtgaa aatctcgtgt accgattgca attgatcatc gataaagcaa
    14161 gatttggaaa gctttctgaa gtagtttcag gagacaaatc acagtttgaa caggtgaaaa
    14221 atctgaactc tggttttgag ttggcctata atgcgccttt tttgctaaga gaatacaagg
    14281 aagcatttga tttagattta gatgtgtcga acttggcaaa agaaagcgaa aatatgtatt
    14341 ttactagaat acaattcgat caaggaaata aaaaaatccg atttttgaat tattcgaacc
    14401 atctcgtata tgaagcaaat atttcgttga attggacaga tatcaataaa gaattaacag
    14461 caaaagacac aaaatggaca gaaatgacaa cagaaccatc gacgatcgac acgcaatatg
    14521 ctacgaaaca ttcgatcaaa ctgaagaagt atagttatat cctgtcacag caaccctata
    14581 cactttttag aaatgctttt ttccagaaac cagatagcgt gaaaaataat gatgaaagca
    14641 atgaattgat tttttacggt gggaatgaaa caatgtctat ccattcggaa acgcaagtag
    14701 ttgattttcg tggagagctg ccagagaaaa aagaaaatgc tagtgtattt tctgaaagtt
    14761 tcccctatat tagcaaatta ggcagttctt tagggaacct cagatatttt gaccgcagtg
    14821 acaatacgat cgattatcgg atattcgtgg aaggcttccc ggtttttggg acagacagca
    14881 gaggacagat tggtgtggaa gttggcggtg aaacgaatgg ttctgtgcaa gtgaatatcc
    14941 aaaccagttt gaatacgatt caagtgccga taccttcaga cgaagaagta gaacttgcaa
    15001 gtacgaatga aatcatccag cagttaaaag ctaagggagc agatagcaac aagattggtt
    15061 cagtgatcgt tggctataca tggcaaaatg tcaaagaaac gaatcgtgtc gttgatctct
    15121 tacctgaatg gtatgtgaaa tataaagata actggtattc agctaacgac cttctggaaa
    15181 aattacctgg attggaggcg aattaattgg atttcaaaaa aatcgaatgg atatttttcg
    15241 tcgctttttt agggttgaat gtgtttttat tcagcagtta tcatgacgcg gtataccaag
    15301 aaaacaatgt cattcgctct aatcagactg aatcaatcca acaacgtctg gctagcgacg
    15361 atatcactta cacggggaag atttcgtctg agaacaagca aggctattat cttagcgcgg
    15421 agcagaccaa tcttagcact tcattagcga attatcgcaa ggctggtaaa agcggcttgc
    15481 tggcaagtgg tgtgttttta gacggtgatg ttttgacgaa atctttatcc agttcagatg
    15541 ctgaaaagaa cgtgatcgat ccagataaaa ttagtagttc cttggaagat tttttatcta
    15601 acaaagacaa tattttattc ggtaatgatt atgtgtacac gaaaaacttt tctcatttag
    15661 aagaagaacc actagaaatc caagcaagcc aagagtatga aggcatccca ttcaatgatg
    15721 atacagcaaa aatcgttctt tctttagaaa aaaacgataa tgattatagc atcacgaaat
    15781 acatgcagac gcatttatct gatatcgagc agctgcgcga aaagacggaa ctgtatacgg
    15841 aagaggaagc aatcaataca ttgtacatca ataacaaaat accaagagga tcaaaaattt
    15901 tatggcgtca gctagcttat tcctgtattc tgaaagtccg ggaaaaaaat gtgtatgttc
    15961 ctgtttggca tgtagcgatc gaaacaagcg ataaagtggt ccaaattgag agcgtcaacg
    16021 ctttcagcaa tacgatcgtg acaaacaata ctgtaccaaa agtggaagat caataaaaaa
    16081 gcgagtataa tgaaaaaagt ttacaaagta agaagggaag taggagtcaa atggctgaag
    16141 aaaaaccagc ttttaaaatc agtatcctag caagtggaag ttcgggtaat tcactttata
    16201 tcgaaagtga aaaaaaacgg cttttggtcg atgcaggact tagcggtaaa aaaatcactt
    16261 ccttgatggc tcaaattgat cggacaccgg accaattaga tggaatactt gtgacgcatg
    16321 aacatcgaga tcatatacat ggcgtgggtg tcttggctcg taagtatcat ttggatgttt
    16381 ttgccaatca gcagacttgg gaagcgatgg aaccactgat tggaaatgta ccagtggaac
    16441 aaaaacatct atttgagatg gggaaagtca gaacattcgg cgatatcgat attgagagtt
    16501 tcggcgtctc ccatgacgca gcggctccgc aattttatcg tttccataaa gaaaatcggt
    16561 cattcgtcat gttgacggac accggatatt gcagtgatta tatgcgaggt gtcattgaaa
    16621 atgccgatgg ttatttgatg gaaagtaatc atgatcttga gatgttacgc atgggacctt
    16681 atccgtggaa tttaaaacag cgtattttag gagatagagg acatctttcc aacgaagacg
    16741 gtgcacttgt catgactgat gtgatcggta atcggaccaa acgaatctat cttggccatc
    16801 ttagcaagga aaacaatatg aaagaacttg ctcatttaac aatggaaaat gttttgaaag
    16861 aaaaagattt tggcgttgga catgattttg aaatctatga tacagatcca gatgaagcaa
    16921 ctgagctatt ttcgatttaa acagttcaat caataaaaat ataaaaagca gaagataaaa
    16981 tcaatcaggg ttggttttat cttctatttt tattttttta gcatattaaa aaaataaccg
    17041 tattatcgac tgactacact gatgtgctgc ttttcatgtt tctcatccag tatctcatct
    17101 actaatgcaa tcgcgtaatc tgccatgctg atcgtactgt cgctagcagc gtttctttgt
    17161 aaataatcat cacttaacaa ataagtacct gtacgtttgc catgtggatc aaataaagaa
    17221 gctggactga tataagtcca atgtagattt ttagattgtt tcaattttaa caatccttcg
    17281 tacatattga aagctgttgg atagaagggt gcatttttag gtacttggtc aatcatacga
    17341 gtcgtctttt ctgtatctaa gaataacgaa gaggcacccc caacgacaat taagcgtgta
    17401 tcggttcctt caagcatctc aatcaaatgg gacaaagaag tttgatgcat ttcttctttt
    17461 cctttgggag cgttgaaagc atcaacgaca acatcaaaag atgcaagatc agtagttgtc
    17521 aactcaaata aatccttttg tataaacgga acatctgatg gcaatttttc tggatgacgc
    17581 acgattgccg tcacgtctaa atctctggac aatgcttcgt ctaaaataaa tgaacctgca
    17641 tggccagtag ctccaatgag tgcaattttc ataaaaaacc tcctttttta ttcctgtcca
    17701 ttgtaagcag ttgctgaata ttcgtcaaag aatccgattt ttgaaaagct cagtgcgcat
    17761 tcctttcatg gttcgtgatc attgggtata gttaaattaa gaaaactgat tgataaaaat
    17821 agaagttgaa ggagataaga aatgaaagag tggttagatc gtgccttttt accaatgata
    17881 acatctgttg tgaatcttag acccttaact gctttgcgta atggaatgac ttacacgatc
    17941 ccaataatta ttgttggctc gctgtttttc ttatttgctc attttcctgt ggattacatc
    18001 gctcagtgga tagacaaaac aggattttct atgtatttta cacaagtata ccaggctaca
    18061 tttgatgtga tagccttttg gatcgttttt gccattgcat acgcttatgc aaaagaagaa
    18121 gacatcgatc gcttaccagt cggcataaca gccttgtctg cttatctact gcttattcag
    18181 ccactaaata ctcagataga tactgtctgg acaggtgctg aagggatact aggagcagtt
    18241 acaactgggc tcatcatcgg atggggctat gcacgtttag taagacttgc gaaagggtgg
    18301 aagcaaagag agaagatacc tgaaaatgtt tggcagtctt ttacttcatt gattccaatc
    18361 attgttatag tcacttttac gcttatcggt tcagcggttt ttcaacattt gttcagaatg
    18421 actgttgttc aaggagtatg gacatatgtc cagcttccca ttcaatcgtt catagacacg
    18481 ttgtttggtg tgcttttatt aggttttttt gttccgtttt tatggttatt tggtcttcat
    18541 ggatctgcga tggtcaatgg gttggttagt cccgtcttgc aagcgaattc attagcaaat
    18601 gcagaaatct tagcctcagg acaagagttg acggtggcaa atggcggaca tatcgttact
    18661 cagcaatttc tcgatcagtt catgacggtt acgggtgctg gattgacgtt aggggctgta
    18721 ttttttatga cggtttttgc gaaatcgaag aaatacagag aattaggaaa tcttgctctt
    18781 ttaccagcct ttttcaacat caatgaatca attatttttt ccacaccgat cgtgatgaac
    18841 ccgatgatgg ctgttccttt tattttcgca ccggtcgttt caggattgat tacctattcc
    18901 gctttatatt ttggattcgt tcctttattt acagcagtcc aagtcccttg gacaacaccg
    18961 ccgatcctct cgggtttctt agtaggcgga gtgcctgcag cgattttaca aggtatcgtg
    19021 ttgtcaatta gttttttcat ttatttccca tttttgaaaa aaatagattc tgcaaactgc
    19081 aaaaaggaaa gacagacaaa aaatgatgga gcagcagaaa agatgaacga ctaaaaaaca
    19141 aaaatactcc tgtcgcttta gattgggaaa atcaccaatc taggatgaca ggagtattta
    19201 tagtcatgtt tatactagcc ctttttggac agctaattga acaagaacaa ctaatgtgtt
    19261 cattcctacg tgtgtgatca tcgatgtcca gatccgacca gttgctttgt actctaggct
    19321 gaagaagaaa cctaggaaaa agtagattag cagatgacca tcattatgag caaacgcaaa
    19381 cagcaaagag ctgatcactg ctccgaccca aaaatttgaa tatcgactga tgatccccag
    19441 tatactgcgg cggaatacga actcttccat gatgggtcca gcaacagttg tcgccaaaat
    19501 aaaagcgggt gcttccatga tcatctggat gatattttgt gtattctcgg aaggggttgc
    19561 tttgccaaac aatacagatt ctaacatgac agcgatgctt tgcagaatca atgcgacaaa
    19621 gattccaacc aatccataaa taacaatctt tggagcagaa gcttttttta ccgctgattc
    19681 gatcgctaag ggttcacgtt gcttaaaata aaaaatagct aggacaacgg cgcctagtat
    19741 atacatgact gcagttgctg tgatcaattc agacgtagat tttacaagtc ctatcgaagc
    19801 taggatcaag ggtgagataa aaattagacc atagcaaaaa atgctgagga agctatattt
    19861 ttttaaagac atgtaattcc tccttttaag ggataacaag catgcaaggc aagcttatat
    19921 cgattgattt caattatatc ataatttcaa taagcagctg tcagaataaa atttcttttc
    19981 tttgatttaa aagcttgcaa aaaagaggga gaatgagtat tattaaaaat gtaggttagc
    20041 actcgaaggt taagagtgct aacagattaa aaaaagagtt atcggaggga tttatcgtgt
    20101 taaaaccatt aggtgatcgc gtcattatcg aagtcgcaaa agaagaagaa aaaactgtcg
    20161 gaggaatcgt tttagcttct gctgctaaag agaaacccca aactgggaca gttgttgcag
    20221 tcggtgaagg ccgtttgtta gaaaacggtg aaaaagttcc tgccgctgta aaagcaggcg
    20281 accaagttat gttcgaaaaa tacgccggta cagaagtaaa atacgaagga aaagaatatc
    20341 tgatcgtcgc aggaaaagat atcatggcga ttgttgagta aatttggaag ataaaaaaat
    20401 aatcaaaaat aatcaatgag gtgaatcaat catggcaaaa gaattgaaat ttgcagaaga
    20461 cgcacgcgca gctatgttac gcggggtaga taaattagca gatacagtga aagtaacatt
    20521 aggacctaaa ggccgaaatg tcgtactaga aaaatcttac ggctctccat tgatcacaaa
    20581 tgatggtgtg acgatcgcaa aagaaatcga attagaagat cattttgaaa acatgggtgc
    20641 aaaacttgta tcagaagtag catcaaaaac aaatgatatt gcaggagacg gtacaacgac
    20701 agcaactgtt ctgacacaag caatcgttcg cgaaggttta aaaaacgtaa ctgcaggtgc
    20761 taatcctcta ggcattcgtc gcgggatcga attggcaaca aaagcagcag tcgaagaatt
    20821 gcataatatc tcaactgttg ttgattcaaa agaagccatt gcacaagtag cagctgtttc
    20881 ttcgggttct gataaagttg gacatttgat tgctgatgca atggaaaaag ttggaaacga
    20941 cggtgtcatc acaatcgaag aatcaaaagg gatcgaaaca gaactagatg ttgtagaagg
    21001 tatgcagttt gatcgtggtt acttatcaca atatatggta acagataacg acaaaatgga
    21061 agctgttcta gaaaacccat atatcttgat cacagacaaa aaaatctcta acatccaaga
    21121 tattctgcca ttgttagaac aaatcttgca acaatcacgt ccattgttga ttattgctga
    21181 cgatgtagat ggtgaagcat tgccaactct tgtattgaac aaaatccgtg gaacattcaa
    21241 cgtagtagct gtaaaagctc ctggtttcgg cgatcgtcgt aaagcaatgc ttgaagatat
    21301 cgctattttg acaggtggta cagtaattac tgacgatcta ggtttagaat tgaaagatgc
    21361 aacaatcgaa aacttaggaa atgcttctaa agttgttgta gataaagaca atacaacgat
    21421 cgtagaaggt tctggagaaa aagaagcaat cgaagctcgt gtgcaattga tcaaaaacca
    21481 aattgctgaa acaacttctg attttgaccg cgaaaaatta caagaacgct tagctaaatt
    21541 agcaggtggt gtagctgttg ttaaagttgg tgcagctact gaaacagaat tgaaagaatt
    21601 aaaattacgc attgaagatg cattgaatgc tacacgtgcc gcagtagaag aaggaatggt
    21661 ctcaggtggt ggtacagctc tagtgaacgt tatcagcaaa gtttctgctg tagaagcaga
    21721 aggagacgtg gcaacaggta tcaagatcgt tgttcgtgct ttagaagaac caattcgtca
    21781 aatcgctgaa aacgctggtt atgaaggatc agtgattgtt gataaattga agaacgtgga
    21841 actaggcact ggtttcaatg ctgctactgg agaatgggta aacatggttg aagcaggtat
    21901 cgtagaccca actaaagtaa cacgttctgc cttgcaaaat gccgcttccg tttctgcctt
    21961 gttattaacg acagaagcag tcgttgcaga taaaccagaa ccagcagcgc cagcagctcc
    22021 tgcaatggat ccatcaatga tgggcggtat gatgtaggaa ttaaaagaga acccttatat
    22081 tacagggttc tcttttcttt ttttgtttta ttttaagtaa aaggggcaaa aaaggggcag
    22141 aaatatcaaa gcctatttag tttatcaatt acttgttgtt ttgctttttt ggttacgtga
    22201 ttgtaaatag acagagttgt atttgcatca gaatgtccta cacgctccat aatggcttta
    22261 agaggtacgc ctaattcaga taatagcgaa acatggctat gtctaaatat atgcgatgat
    22321 agattttttt ctaattccaa ctcttcttct actttatgga gtacagcgtt aaatgagtgc
    22381 agcgtaagcg gagtgccatt tgtagatata aatatatatt gatcgggatt ggtgggtcta
    22441 cctgcaagaa tattgtcagc tatcacactt tcaatcaatt cttttgcacg attggataat
    22501 tgtacttcac gttgtgagta agtatttttt ggagttgttt ttatggcgtt atccattttt
    22561 acagatgtgt aatctaaggt cccattaatg gaaattttac cctctccata gtccttcatt
    22621 tgcaaagcca gcaattcccc atatctcaaa ccagtcaaat atagaaattc agctattatg
    22681 ccgtgtagtt ttctgcgagg attggaatat aactgtttca gtatttgatc aatttcttct
    22741 ttctccagat attttttatc catagaaagc tttctttttt cttcttccac ttttttagga
    22801 tggattttaa ctgctagagc agggtttctt tgaatgtatt ttctatcaat tgcatagttt
    22861 agcataacgg atagagttgt ttttgtttgc tttgtgtagt tcagtgagag atcaccaaaa
    22921 gtgtacatat cttcaatcat cttattaatg agtgtctcat caatgtttct aacgattgta
    22981 tcatcgctta tgtgcttaga aacatgtttc atcatcattg gaaccttcaa atagctagta
    23041 cgtttaacat gctgcttata atattcatac cattctttat acagctcacc aaaagtgata
    23101 tctgatttat tgtaattttc gagtgcttct ttaatttttt tatctagaat tttctgagct
    23161 tttttccacg cttgtggtga attacttgta agtgttgtag atttttttcg tgttttttct
    23221 gtataaggat ctacatatct ttcaataaac ttgaattggc catctttggt ttgttcaacc
    23281 cacactttta acatctccta tcatttgcta taataggcat aacaaataga cctatatagg
    23341 tttgttttct aaaagcacgc tcttactctg gacggtgggg cgtgtttttt atttattata
    23401 cagttgcttt aaaaatagct gctttttcaa atgatactaa aggagagaag tggatttcta
    23461 tttctccctt ttggtttaac gtgaaatgag cagttacatc catttgtttt cctggagcaa
    23521 cagatcccat tgtattttcg ttggcatatg tctctgattt tttgtcatct ggtccatata
    23581 cttccacatc tgtacctaca gggatatctg aatcgccatc attttttacg gtataagtaa
    23641 tttttactac ttgtgcaggt tggttttctt caaattgatt tctttcatca gttagttcta
    23701 cactatttag tgtatattca gcatcaccaa cttttacagt gtcaccaatc ttatagaagg
    23761 tatcgctttt ttcttcttta gaagatgaag cagaggttga agatttagtt actttttcgc
    23821 caccattatc acttgcttta ttactatctg atccaccatt taaagcagaa ccaataatta
    23881 taattaaaat tactgctaat acccaaaacc atactttttt gtaaaaaggt ttactaactt
    23941 tatacatttt tccgtcttga cccataactt tttttgccat ttaaatattc ctcacttctt
    24001 gttataatat atttgcgatc tcagaaatga ggtatgagtc cgtgtg
//
LOCUS       DAYYKN010000032        23741 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000032 DAYYKN010000000
VERSION     DAYYKN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.032"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(189..1115)
                     /locus_tag="ACHBB7_001072"
     CDS             complement(189..1115)
                     /locus_tag="ACHBB7_001072"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM9209037.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(1200..1961)
                     /gene="pflA"
                     /locus_tag="ACHBB7_001073"
     CDS             complement(1200..1961)
                     /gene="pflA"
                     /locus_tag="ACHBB7_001073"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM9209038.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(2205..4436)
                     /gene="pflB"
                     /locus_tag="ACHBB7_001074"
     CDS             complement(2205..4436)
                     /gene="pflB"
                     /locus_tag="ACHBB7_001074"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM9209039.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(4713..7163)
                     /gene="parC"
                     /locus_tag="ACHBB7_001075"
     CDS             complement(4713..7163)
                     /gene="parC"
                     /locus_tag="ACHBB7_001075"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM9209040.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(7175..9220)
                     /gene="parE"
                     /locus_tag="ACHBB7_001076"
     CDS             complement(7175..9220)
                     /gene="parE"
                     /locus_tag="ACHBB7_001076"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM9209041.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(9386..9820)
                     /locus_tag="ACHBB7_001077"
     CDS             complement(9386..9820)
                     /locus_tag="ACHBB7_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM9209042.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            9947..10585
                     /gene="plsY"
                     /locus_tag="ACHBB7_001078"
     CDS             9947..10585
                     /gene="plsY"
                     /locus_tag="ACHBB7_001078"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM9209043.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(10717..11592)
                     /locus_tag="ACHBB7_001079"
     CDS             complement(10717..11592)
                     /locus_tag="ACHBB7_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM9209044.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(11674..12465)
                     /gene="codY"
                     /locus_tag="ACHBB7_001080"
     CDS             complement(11674..12465)
                     /gene="codY"
                     /locus_tag="ACHBB7_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM9209045.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(12483..13883)
                     /gene="hslU"
                     /locus_tag="ACHBB7_001081"
     CDS             complement(12483..13883)
                     /gene="hslU"
                     /locus_tag="ACHBB7_001081"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM9209046.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(13897..14445)
                     /gene="hslV"
                     /locus_tag="ACHBB7_001082"
     CDS             complement(13897..14445)
                     /gene="hslV"
                     /locus_tag="ACHBB7_001082"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM9209047.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(14482..15387)
                     /gene="xerC"
                     /locus_tag="ACHBB7_001083"
     CDS             complement(14482..15387)
                     /gene="xerC"
                     /locus_tag="ACHBB7_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM9209048.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(15585..16961)
                     /gene="trmFO"
                     /locus_tag="ACHBB7_001084"
     CDS             complement(15585..16961)
                     /gene="trmFO"
                     /locus_tag="ACHBB7_001084"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM9209049.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(16930..19008)
                     /gene="topA"
                     /locus_tag="ACHBB7_001085"
     CDS             complement(16930..19008)
                     /gene="topA"
                     /locus_tag="ACHBB7_001085"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM9209050.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(19130..19987)
                     /gene="dprA"
                     /locus_tag="ACHBB7_001086"
     CDS             complement(19130..19987)
                     /gene="dprA"
                     /locus_tag="ACHBB7_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM9209051.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(20043..20810)
                     /locus_tag="ACHBB7_001087"
     CDS             complement(20043..20810)
                     /locus_tag="ACHBB7_001087"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM9209052.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(20803..21663)
                     /gene="ylqF"
                     /locus_tag="ACHBB7_001088"
     CDS             complement(20803..21663)
                     /gene="ylqF"
                     /locus_tag="ACHBB7_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM9209053.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            21923..22321
                     /locus_tag="ACHBB7_001089"
     CDS             21923..22321
                     /locus_tag="ACHBB7_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9209054.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            22558..23100
                     /locus_tag="ACHBB7_001090"
     CDS             22558..23100
                     /locus_tag="ACHBB7_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM9209055.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(23183..23734)
                     /gene="lepB"
                     /locus_tag="ACHBB7_001091"
     CDS             complement(23183..23734)
                     /gene="lepB"
                     /locus_tag="ACHBB7_001091"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9209056.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
BASE COUNT         6229 a         5080 c         4130 g         8302 t
ORIGIN      
        1 agttcatata tgttctcttt tctatccgtt ttattttact taaaaggctt ttacgtaaaa
       61 aggaggaaga acaagagtca taattgactg ttattctcct ccatttcttc ggcctttagt
      121 atgttctatg tttcttttta tatcaatcgc ttatccattc attttgaatg cttcatacta
      181 ccatttgatt agctaaatac agccgtcaat tgaggtacga cttgtttttt acgtgaaacg
      241 acacctttaa gcaaagcacg gttgttggtc aaagttgttt ggaaagcttc ttctactttt
      301 tcttttcctt taccagcaac aagtaattct gaatcactgt ctaagatgtt tgtgacaact
      361 aatacgaaca gatcgtagcc atttgctttc atttcatctt ccattgctgc ttctagctct
      421 gcttgtcgat ccatcacttc attaaggtcc actgtattga cttgagcgat acgaacgttg
      481 gaactgccca ttgggaaact ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca
      541 cttagatttg ttcctgcttt caacatttca agtccataag tgtttagatc gacttcagcg
      601 attgctgcaa gttcttttgc agctgcttca tcttcaggcg tacatgttgg tgatttgaac
      661 aacaatgtat ctgaaatgat agctgagagc atgattcctg cgatttcttt aggaattgtt
      721 acttggtttt ctttatatag ttttaaaata atcgtacttg tacatccaac tggttctgca
      781 cgatagtaca atggattagc tgtttcaaag tttgcaatac gatggtggtc tactacagct
      841 aaaatattta cttcgctgat atctgaaata ctttgttgga attcattatg atccacaagc
      901 atgacatctg cattttctgc tttagctgac tctactactc gtggtgcaga tagctgaaag
      961 tggtctaatg cgtattgtgt ttcttcatta ggtgtaccta gagcaactgc ttctgtttct
     1021 ttacctaatt cattttgcaa gtgtgaaaac gcaatggctg ccccgattgc atctgtatct
     1081 ggattttggt gaccaaaaac taaaattttt gacatgtcat tcgctccttt gtgatttctc
     1141 ctatttaata atagcaaaaa aacggaaaga agcaatactt atcttctttc cgatgcgacc
     1201 tatcgcgtta aatattttgt atactcttct gtatgaagca atcgtttagc attttcgaca
     1261 cgttcttttg taggtggttc gatcccttct aatggatatt tcaaaccaag tgtttcccac
     1321 ttgtattttc ccatcgtatg ataaggaaga atctctactc tgtctacatt tgacaaggtt
     1381 tgaataaagt tgtctagtcg gatcaaatat tcatcataat cacttcgctc aggaactagt
     1441 acatggcgaa tccaaaccgg tttgttgatt tttgacaaat attgagccat ttctaaaata
     1501 ttttcattgt cccaatgcgt tagtttttta tgctcttcat tatcgatgtg tttgatgtca
     1561 aataacagta aatctgtata cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca
     1621 tacgtaaaag gttttccgca tgtatccaat gtagtatgca tcttccgctc ttttgctttt
     1681 ttaaatagtt cgatcaaaaa atcgatctgc aatagaggtt ctccaccact aacagtaata
     1741 ccacctttat ctccccagta aggtctgaag cgttctgctt tatctaaaag ttcatctgct
     1801 gtgtattctt tccctccgcc aatattccaa gtatctggat tgtgacaaaa ttggcaacgc
     1861 atgcggcatc cttgcataaa cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa
     1921 gtttcgatag aatgaacata gccgattgtt ttttcttcca tttaagttca catcttttct
     1981 ttgatttcat tttaagtaca agttagctgt cgaagcgaag ctttctgaac aaaatttttc
     2041 gcaattgccg cttcgttatg tgtaaaattt acagggacgg aacatacaag tcctgcccct
     2101 gtaaatcgtt tctttcaaat aagcatactg attattcgat tttgatgcga tggctattca
     2161 acgtcctttc gacgctaagt cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt
     2221 gaagttctca tcaagacatc taattgttgt tctcttgtca agtcacggaa tttaactgca
     2281 taaccagata cccggatcgt caagttcgga tatttcattg gattttccat tgcatcacgc
     2341 aataaatcat tactgaatac attgacattc aaatgataag ctccttttga gaagtatcca
     2401 tctaagacat tacgcaagtt ttcgattctt gtatcttgtt cttttccaag acctgaagga
     2461 ttgattgttt gggtatttga aattccatcc aatgcacagc cgtaattcat tttagctgtt
     2521 gagttcaggc tagctaataa cccactttgt tccgcattgt aacttggatt tgctcctggc
     2581 gcaagtggtt ttccagcttc acggccatca ggagtatttc ctgtattttt accgtaaaca
     2641 acatttgaag taatcgtcaa taatgaagtt gttggttttg agttgcggta agttttatga
     2701 cgtttgattt gtcccatgaa gtattccaag atccatgaaa ctaattcatc tgcacggtca
     2761 tcatcatttc cgtatttagg gaagtcgcca tccacttcaa aatcaactgc tattcctttt
     2821 tcattacgga ttgcttttac ttttgcatat ttgatcgctg acaagctgtc tgccgcatga
     2881 gagatcccag caatacctgt agcaaatgtg cgttgcaagt cagaatccat caaagccagc
     2941 tgagctgctt cataagaata tttatcatgc atataatgaa tgatgttcaa cgtattgaca
     3001 tatagttctg ctaaccaatc catcatattc atgaagttat gtttcacttc ttcaaagtct
     3061 agtatttctg atgtgattgg acgatattct ggtccaactt gggcgccagt cacttcatcg
     3121 actccgccat tgatggcata taatagtgtt ttagccaagt tagcacgagc gccaaagaat
     3181 tgcatgtctt ttccgacaac tgttcctgat acacagcaag caatcgcaca atcatcagaa
     3241 ccccattgtt cacgaagctg attatcattt tcgaattgaa tcgaagaact ttcgatagcg
     3301 attctgctag catatgttct gaatccttct ggaaggtgtt cagaatacaa aacagtcaag
     3361 tttggttctg gtgaaggtcc catatttgtt aatgtatgaa gcagacggaa atcattcttt
     3421 gtaactaatg aacgaccatc taatcccata cctgcaatgg ataaagtagc ccagataggg
     3481 aatcctgaga ataattggtt atattcaggt gtacgcgcga atttgaccat acgtaatttc
     3541 attactaagt ggtcgatcaa ttcttgtgct tctttttctg tgattactcc attatccaaa
     3601 tcacgttgga tatagatatc aaggaatgct gagatacgtc cgattgacat cgcagcacca
     3661 ttttgtgatt tgattgctgc taaatatccg aagtatagcc attggacagc ttcttttgca
     3721 tttgcagctg gtttagaaat atcatagcca tatttcgcag ccatttcttt catttgcgct
     3781 aatgcacgat attgttcagt gatttcttca cgtaaacgaa tcacttcttc actcattgta
     3841 tgataaccag tcatatcatg atcttttttc ttttgttcca tcaaatagtc gattccatac
     3901 aatgccacac gacgatagtc accgatgata cgtccgcgac cgtaagcgtc tggaagccct
     3961 gtaatgattt tgtttttacg agcagcacgc atttcagcag tataagcgtc aaatacacct
     4021 tgattatgtg ttttgcgcca gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag
     4081 ccattgcttt ttagcgcatt gttagccata ttgattccgc cgaacggcat gaatgcttgt
     4141 ttcaaaggtt tgtctgtctg tagaccgacg attttttcca aatctttatc caaatagcct
     4201 gcatcgtgtg aagtaatagt tgatacgatt tctgtgtcca tatcatagac accgttacgc
     4261 tcgaattgga tatcatttaa cttttgcaat tcatcccata atttttctgt tgcttctgtt
     4321 gggccttcca aaaagctgtc atcaccgcga tattcagtgt agttattttg aataaagtct
     4381 ctagtgtcta cgccttcttt ccatttggta cctttaaatc cattccattg ctccattgta
     4441 tggacctcct aaatttctgt tttatgtaac agtttttacg tgattagtat aacacagcac
     4501 cctatctttg caagggtttt cttgtttttt atttaacaat ttttatgttt tatttacata
     4561 tatttaaatc aacgttgaca taccaacatt taagcattgt gaacactttc atcttttttt
     4621 gcaaacgtat aaggatttat taaaaatgaa tgaaaaagag acgccacctg tattaataca
     4681 gatggcgtct cttcattaaa atactacaac agttacgact cgatgaaaat ctcaggagca
     4741 tcgataactt cgatgacttc tccacctttt ttctcatcca tcgcaaatga tccattcgat
     4801 gttcgatcac ctatagggaa atcagttggg tcaatcgtta actgctgccc tttttgattc
     4861 atcaaggtta tttcttttga tcttccttcc gacatataga taatacggtg cggatttttt
     4921 ttcagctcac gcatgaccgt cactccccgc ttcgcacgtg aagtttggct gatttcttga
     4981 gcaagcatgc gtttaacagc tcctcgctga gtaacgatta cgatcggcgt atcaccttct
     5041 gctaacacaa gcagcccgtt gataatccta tctccatctt tcaaattcat agacttcact
     5101 ccagctgcct ttgctccaac aacgggaaca tctgttaaag gatagcgcaa acccattcct
     5161 tgatgcgtta ctaagaaaac atctaagcct ggctgatctt ctttcagata cacagcaatc
     5221 agttcatctt cttccgcctt tagcttcata cagctgagcg gacggctctt ataggtgcgc
     5281 catggttcga actctgtcat tcgtgtttgc ttgatcaatc cattcttgct gatgaataca
     5341 aatgttttct ctgcttgaat tttttgatat gggaaaacag ctaaaatcga ttcgtctaca
     5401 gacagattgg agattgtttg agaaatgtgc tcaccagcat ctttccattt caaatcgggt
     5461 agttcatgga cagggcggta gatcacattt gctttatttg tgatcagaag aacatggtcc
     5521 aatgtactca attcacctga gtatagcaag tagtcgcctt ctcgcatccc gatttcttct
     5581 ggtttggaag cactataaga acgaaggctt gttcgcttca catagccttc tcgcgtcaca
     5641 ctgacaatta cgtcttcttg tgccactaag acttgcgtat caattttgat ttcttcgatt
     5701 tcgtcttcga ttgtcgttag acgagcggaa gaataatttt ttttcacttc tcgcaattct
     5761 tttttcataa cagagaaaag ctctttgtca tttgaaagga ttttattcaa ctctgtgatt
     5821 tgcgcaatca agctttcgga ttccttttga agctctgtga tatccgtgtt cgtcaaacgg
     5881 tataattgca atgtgacaat tgcttctgct tgttcttctg taaattgaaa gacgtcgact
     5941 aaattctttt tggcgtcttt tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc
     6001 agaatcgata aagctttcat caatccttct acgatatgct gtcttttttg agctttggct
     6061 aaatcgaatt gactacgttt tgttatgact tgctttcgat gctcgatata acttcttaaa
     6121 atgtctttca gtccaacttg atgcggagtc atatggtcga tagcgaccat attgaaattg
     6181 taattgatct gtagctcagt gtttttgaac aaataattca aaattccttg agcattggcg
     6241 tcttttttca attcgacgac aatttgcaga cctgttctat cactttcatc tcgtacttca
     6301 gctattccat cgattttctt attcaaacgg atctcatcca tttttttcac caaggtcgct
     6361 ttatttactt catatgggat ttctgtgatc acgatttgct gtttattacc cttcattgcc
     6421 tctatctttg ttttcgaacg aagaataact tttcctcgtc ctgtttcata agcttttttg
     6481 atttcatctt ttccttgcaa gatccctcct gttggaaagt caggtccagg aatatattcc
     6541 atcaattttt caaggctcgc ttgtggatga tcgatcatat agattgtgcc atcgatcact
     6601 tcagctaagt tatgagtcgg gatttctgtt gcatatccag cagatatacc tgtcgatcca
     6661 ttgacgagaa ggtttgggta tcgagctgga aggactgttg gttctttttc tgtatcatca
     6721 aaattccaca cgagatcgac tgtttctttc tcgatatctg ctagcatctc tccacttaac
     6781 ttggataatc tggcttcagt ataacgcatc gcagcaggtg gatcaccgtc catactcccg
     6841 ttattcccgt gcatttcgat cagtacttcc cgcaacttcc agtcttggct catgcggacc
     6901 atcgcttcat aaatactgat gtcaccatga ggatgataat tccccatgat atttccgaca
     6961 gattttgctg atttacggaa tcctttgtca tacgtattgc catctttgtt catggcaaat
     7021 aatatccggc gttgcactgg cttcaatcca tcacggatat caggcagtgc acgttcttgg
     7081 ataatatatt tggaataacg tccgaagcgg tcacccatca cttcttctaa agttaattct
     7141 tgtacttctt gtcgattttc catcaggtga cctcctattc tacttcaaat aactgattgt
     7201 tttcattttt atctgctcgt tcttcatcta ataaatcatt agaaactgaa ggagagattt
     7261 cagtatcctc ttttttatct aagatactgc catcttcttc gagcgtaaat tgcacgtgat
     7321 tttcgatcca ttttcttcga ggttctactt tgtcacccat cagtgtggtt actcgacgtt
     7381 cagcttgcgc agcatcatcg atacggacgc ggatcaacgt acgggaagtc ggatccatcg
     7441 ttgtttccca caattgttcc gcattcatct ctccaagtcc tttatatcgc tgcagcatat
     7501 atcctttgcc gactttttta atcattgctg ccaattcgtc atctgtccac gcatattcaa
     7561 tgacttgctt ctttccttgt cctttggata ctttatataa aggcggtaac gcgatatata
     7621 ctttcccagc ttcaatcagc ggtttcatat aacgataaaa gaaagtaagc agtaatactt
     7681 gaatatgggc gccgtctgta tccgcatcgg tcatgatgat tactttgtcg taattacagt
     7741 cttcgataga aaactcagga ccgactcctg caccaatcgt atagatcatc gtattgattt
     7801 cttcattttt caagatatct tgcattttag ctttttcggt attgatgact tttcctcgta
     7861 aaggtagaat tgcttggaac tttctgtctc ttccttgttt agcagatccg ccagcagaat
     7921 ccccttcgac taaatacaat tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt
     7981 ttcctgaaag aagagattcg ccttttttac gcttcttccc atttcggctt tcttctctgg
     8041 ctttacgtgc tgcttctctc gcttctctgg ctttgattgc tttacggacc aacatctggc
     8101 tcatttcgct attttcctgc aaataaaagc ccatttgttc actgatgacg ttatctacta
     8161 cggtacgagc aactggcgta ccaagttttt cttttgtttg cccttcaaat tgcagaagat
     8221 tttcaggtac acggatagaa agtacagcag ctaacccttc tctgaaatca ctaccttcca
     8281 gattcttgtc ccgttctttc agcaaaccaa cttttctcgc atattcatta taagatttcg
     8341 tcatcgccgc tttcatacca gcttcatggg ttcccccatc ttttgtccga acgttattga
     8401 caaaagataa aacattttct gaataaccgt cattgtattg ataagcgacc tctacttcga
     8461 taccttcttt ttctcctgaa aaataaacaa ctggtgtcaa tgtatctttt tcttcattca
     8521 gataatcaac aaattctttg attccttctt cgtaatgaaa aatttccttg actggttctt
     8581 ctccacgaag atcagaaaga gagattttaa cgccttttag aagaaaggca gattctctta
     8641 accgttcggc taaaatttca tatgaaaatt tcgttgtact gaatattgta tcatctggta
     8701 aaaaatggac agtcgtcccg tttgcttttt ttgtttttcc tatctttttc aaagtaccgt
     8761 caggtttacc gccgttttta aatttctgct gatactctac gccatctcta acgatcgtta
     8821 caattagcca tttcgataag gcatttacca cactggctcc tacaccgtgc agtccaccag
     8881 aagttttgta ccctccttga ccaaactttc cgccagcatg cagcacagta aaaatcactt
     8941 caactgttgg tattccggaa gcatgcattc ctacaggcat cccacgtccg ctatctgcaa
     9001 cagttatact attgtcttca tggatggtca catcaatctc actgccataa cctgataatg
     9061 cttcgtcaac tgcattatcg acgatttcat agactaaatg atgcaagcct cggctgtcag
     9121 tggatccaat gtacatccct ggtctttttc taactgcctc taatccttcc aatacttgga
     9181 tggacgagtc attgtattcg ttgtttactt tttttgccaa ggcaaacgct ccttcaataa
     9241 tactttgcgg caaaaccgca ccacgattat catactagaa aaggtcgtga aaaaaaagag
     9301 ggtagacgag aaatcaattt taattatctc ctaaccgctt tttcgacatt cccctctatc
     9361 gatcatctct tctttgtggt catttttatt tttggttttc tttcaactta ccaagttcaa
     9421 tcttaataca acgatccatt acgatatctg tacgtcctgc ttttcttagc atctcagctg
     9481 cttctttgtt ttcaatccct aattgtgccc agaacacttt agcatcagtt ttcaaaaaat
     9541 cacctgctat ttcaggtaaa aattcactcc gtctaaatac atctacgata tcgattggat
     9601 catcgatttc tgtcaaattc gcatagactt tctcgcctag aatttcttca ccagccaata
     9661 atggatttac tggtatgatt ttatatccat tgtcttgcaa tacctgtgca accatgtaac
     9721 ttgttcgttc tggtttattg ctcaagccta cgacggcaat attttttgct tcttccaaat
     9781 agttgaaaat cgtttcttgt gttggatttt ttacagacat ttctgccacc ccttgctttt
     9841 ctttttattc catctattat acttgtatct ttcatttttt caattgttcc tgttacctat
     9901 tcatgctaaa ataaaggata gttttcaact tgaaagaggg taaaaaatga aattgatctt
     9961 attacttatt ttagcatatc ttttaggttc tattccttcc ggtgtctgga ttggcaaatt
    10021 gtttttcaaa aaagatatcc gacaacacgg gagcggtaat actggaacga ccaatacatt
    10081 tcgtgtatta ggcaaaacag ccgggattgt tgtcctgctt atggatattt taaaaggtac
    10141 attggctact tgtttgccga tgatcttcca cctttctggg attaaccctc tttggtttgg
    10201 ggtatgcgcc attcttggcc atacatttcc gatttttgct gggttcaaag gaggaaaagc
    10261 tgttgcgaca agtgcaggaa tgcttttagg atttaatcct gtattttttg tttattcttg
    10321 tatcatcttt atcgttagtt tattttgtac aagtatggtg agtttgagca gtatgatcag
    10381 tgcagtattg atcactttat ctacgatcgt ccttccttat gtagctcctg tgattttggc
    10441 taagcccaat tggttactga cgatcattgc ctttttagtt tcttccttta tcttttatcg
    10501 gcataaagac aatatcaagc gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt
    10561 gaatagcagc aagaaaaaat cctgatactt tttcattcca aagagataaa acgggagtgt
    10621 gacaaatcga tgtcacactc ccttattact tacaggatag cttttttaca caccttggta
    10681 aacggtgttc cctgctaact tctcggtcac aacctcttac ttcactgtga tggaatattt
    10741 tgtcttgaag atttcatggg cacctagttg attgatccct actttttcca tcaagtttcc
    10801 actactgttc accgtgtcag cgattccgca ccacggttcg atacaaacga aaggcgcttc
    10861 ttttggataa ggagaccaga tccctacata aggcatatct ttatagctta aagttacgct
    10921 atgggaagat ttttcacttc gaatggcgta cgcatttaga ccccttgttt caaaaaccaa
    10981 ggcatcttgc tcaaaaagtt cacgattcaa agccagattc gtatttgtct gacctaacgt
    11041 cttttgatcc atatcgatga atgggccttt caatggtagg cgtaatcttg atttcaatgg
    11101 agaaaatgcc agatagaaat cttcaaatga caatccttct tcaagaggta cattgaaagc
    11161 aggatgtcca ccgatagaaa agaacatttc tttttcaccg gtatttgcca cttgataatg
    11221 aaccgtgatg ccgtctcctc ctaattcata gctgatcact aattcaaaat caaatggata
    11281 cttttctaat gtctccggcg tactccttaa gatgaaacta gccatttctt gtccatgttc
    11341 aatgacttca aaatccatat cccgagcaaa tccgtgttgc cccatttgat atgttttccc
    11401 ttgataaaca tactgatcat cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg
    11461 tctgccccaa tacgctggat caccttgcca gatatactct aaaccatgtt cttttgattt
    11521 cagactgatc aattccgcac cgtgttcagc gatttcggcg atcagctggt cattttgaat
    11581 aataacagtc attgccattc ctccaacttc gacttacttt ttaaaatggg aaggctggaa
    11641 caaacatttc tatctgcccc agtcccctta cttctaataa ctgtgttttg ctaactcgtc
    11701 tttaaaacgg gaattcaata cttttaaata agttcccttc atgcctaacg aacgagattc
    11761 aatgattcca gctgattcca attttcgtaa agcattgacg atcacagaac gagtgatgcc
    11821 gatactatcg gcaatattag aagcagtcag acgaccttca tctccatcca atgcttcaaa
    11881 gatagcttgt acagccttca attcgctata agataatgtg ttgattgcca tttggacagc
    11941 cgttgcactc cgtacatctg cttcgatgct tcgagacttc tgataaagga tctgcatacc
    12001 gacaaccgta gctccatatt ctgctaagac tagatcgtca tctgcaaacg attgttccat
    12061 acgagataag ataatcgtac ccaaacgttc gccagctcca aagatcggaa cgacagtcgt
    12121 cagaccaaat ggatatttct ctcttgactc taccggaaat gcagtaatat cactagtaat
    12181 tgggatatta gcttcagttt tcatcaactg tgccactgta tcagtataag attcaggaaa
    12241 ttgtttctca acaaacattg ttttgattcg ttcattgttc acatcgtgac gttcattata
    12301 acctagtaat actccgtcac ttcctatgat ataggtgttg ctgtcaagaa taccacctag
    12361 tgtgacagcc attttatcgt aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg
    12421 caaaagtgtg tttatcttgc gggttttcgt tagcaagtct gtcattgtcg tcctccgatt
    12481 ttttataaaa tatagcgact taaatcttca tttgccgcaa ttgagccaag tttgtcatcc
    12541 acatatgctt ctgtgatcgt tatttctccc atttgcatat ctggtgcttc aaataataga
    12601 tcttctagta gtttttccaa aatggtatgc agtctgcgtg cgccgatatt atctgtgtca
    12661 cggttgacat cgaaagcgat ttgagcgatc cgttcaattg cttcttttgt aaacgtaact
    12721 gacacatttt ctgtaccgat caacgcgacg tattgtttta ctaaagcatt atttggttcg
    12781 gtcagaatct tcacaaaatc ttcagctgtc aaatcatcta attctacccg tatcgggaag
    12841 cgtccttgaa gttctggaat tagatcactt ggtttagaca aatggaaagc tcctgaagca
    12901 atgaatagga cgtgatctgt ttggatgatc ccgtattttg tgtttacttg tgaaccttcg
    12961 acaattggca atatgtctct ttggacgcct tctctggata cttctccaga ttgctgtgat
    13021 ttagaagtga tcttatcgat ttcatcgata aaaataattc cactttcttc cgctaatttg
    13081 attgcctcac tatggatatc agcatccttg actaattttg ctgattcttc cttgatcaat
    13141 aactctaaag cttctttgac tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca
    13201 cctagagttt catttaggtc gatccccatt tgctccaatc cattgttcat cattggtgca
    13261 ggtttctttt ggtcttcaac ttcaatggtt acttcacgac tatccagtaa acccttttgt
    13321 agttgttcca agactgtacg gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt
    13381 tcagcggtat tttgctgact agcattaaaa agattcatca tttgttcaaa ttgattagtg
    13441 gattgtttct gttctttttt gataccaggt accaagattt ttaccaaacg tttttctgca
    13501 ttttttttag cacttgtgta tacacggctg tactgctctt tttctacgat ttggatcgca
    13561 ttttctacta aatcccgaac cattgattct acatctcttc cgacataacc gacttcagta
    13621 aattttgttg cttcgacttt gacaaatggt gcattgacga tttttgccaa acgtcgtgcg
    13681 atttctgttt taccgacccc tgtaggtccg atcatcagca tattttttgg tgtaatatct
    13741 tgttgcattt tttcatctaa ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca
    13801 gatttttttg ctgcgtgctg tcctacgata tattcatcta attcacttac gatttgtctt
    13861 ggtgtttgtt ttaattcata catatctact cacctcttac agttcttcta cgataatatt
    13921 atgattagta aagatacaga tatctgcggc aatatttaac gcattttctg caatttcttt
    13981 cgcactcatg tcgttttgag catagttttt cattgctcgg gctgcagcca acgcaaaatt
    14041 ccctcctgat ccgatagcta agataccatc atctggtgtg ataacttctc ctgtacctga
    14101 aacaagaagc atttctttat cattcattac gatcagcatc gcttcaagtt tttgcataga
    14161 ttgctgtgtc cgccattctt gagctaattc gacagctgcg cgttgtagat tcccgttata
    14221 ttcgtttagt ttgccttcaa atttttcttc taaagtaaac gcatctgcaa cacttccggc
    14281 gaaacctaca acaacttctc cattgtagat acgtcgtact ttctttgcag tgcctttcat
    14341 gacaacagat tcacccattg ttacttggcc atctccagcc atagcaaatt tcccgtcttt
    14401 ttcgacggca cagatagtgg ttgaatgaaa tggtgattcg accatgtttg tttcctccta
    14461 aatttgttct tttccttatt tttacgctct tggatggaaa gaacgatagt ttttttgtaa
    14521 actttctttt gttacatggg catagatctg ggtagtcgat aaattggcat gtcctagcaa
    14581 ttcttggact gtccgcaaat ctgccccatt atttaataaa tgagtagcaa atgtatggcg
    14641 cagcatgtga ggatggatat cactatccaa actgcttttt ttgatcattt gattcaagac
    14701 atattcaatt ccagtaggcg tgattggttt tccatgatga ttcacaaaaa cgatatcatg
    14761 ctcttcgtga tattttgtca tcaatacctg gcggccttcc ctgaaatatt cttgcagagc
    14821 atcttgagca aaagaaccta gaggagcata tctctctttg ttccctttac catggatcaa
    14881 catgacctct gaatcaaaat caatgtctga taccgacaaa ctgctacact cacttaaacg
    14941 gatgcctgac ccgtaaagga cttccagtaa agcgcggttc ctcaaatcca aaggactatc
    15001 tcctgctacg ctgtcaaaaa gtgcttgcat ttcattttca taaaaaaaac gtggcagtct
    15061 caaatttttc tttttgagat gaacgtaaga aaatggattt tcttttatca cttcttcttt
    15121 gagaagatac tgataaaacg aacgtaggct ggctatcttg cgactgatag aatttctgct
    15181 atagctagga cgatgatcag ctaatgcact caaatagaca cgaaccgtaa aatattccgc
    15241 cttcagcaga tcagcgtctc cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc
    15301 ttcatatgct tctttcgttt tttctgaata tcctctttcg acaatcaaat aacgcaaaaa
    15361 tttttgttcc cacgtggctt ctgtcatttt ctcaccttcc ttttgtccca gagaataatt
    15421 tagcacagcg tttataaaaa gacaaataaa ttgtcagaat ttaaccaata tttctaagtt
    15481 tgttcacaat tggcgcaatt cctcacattt tgtttgagaa attcttaaga aaaacgcaag
    15541 aaaaagagcg agactatccg tctcaccctc ttcatggagt ttccttactc atttgggatt
    15601 gcagtttttt ctaattctgt ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt
    15661 gcttcatatc ttgctttttt gtctcgaatg cgctctggca gttcaggaaa cagaccatag
    15721 ttggcattca tcggttggaa atgttttcct tccgcatgag taatgtagta agccatactt
    15781 ccaatagctg tttcttgtgg aaactcgact ggttccattt cttttgctaa gcgtgcggcg
    15841 ttgattccag caagtagtcc gctcgctgca ctttcaacat agccttctac accagtcatc
    15901 tgacctgcaa agaacagatc ttcgcgtttc gttgattggt aagtcggttt cagcaattct
    15961 ggcgaattca taaagctgtt tcggtgcatc acaccatagc gaacgaattc agcattttct
    16021 aacccaggaa tcatccggaa aacacgtttt tgctcgcccc atttcaagtg agtctgaaag
    16081 cccacgatgt tgtataaaga agctgcagca ttgtcttgac gcagctggat aacagcataa
    16141 ggacgtttgc cagttttagg atcttccaat ccaactggtt tcataggtcc gaaaagcatc
    16201 gttttgaatc ctcgctgtgc cattacttca attggcatac atccttcaaa atatttttct
    16261 ttctcaaaag atttaagttc tgccacttcg gcattgatta gtgcctcatg gaaagctttg
    16321 aattcctctt ctgtcatcgg acagttcaag taagctgctt ctcctttgtc atagcgggac
    16381 ttcaaatata ctttatccat atcgatcgta gctttgtcta cgattggtgc agctgcatca
    16441 tagaaataaa aaccatctga accattgaat gctttgatgc tttctgctaa tggatgagaa
    16501 gtcaaaggtc ctgttgctac tacggtgatt ccttccggta tttcagtaat ttcttcgttt
    16561 atcactgtta ctaatggatg ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg
    16621 cggtcaacag ctaaggcacc accagctggc actgctgttt catctgccga agtgattact
    16681 accgaattca atcggcgcat ttcttctttc aataacccta ctgcattagc caaactgttt
    16741 cctcttaatg aattggaaca tactagttca gcaaaattac ttgtgtgatg cgcctcagtc
    16801 gatttgactg gacgcatttc atataaacgg acagggactc ctgcttgtgc gatctgccaa
    16861 gcagcttcac ttccggcaag ccctgctcca acaacattta ccattttcaa tgaaagaacc
    16921 tcttttctat tatttttgga tgtcttcttc gtaatctcca tttacacaga ctacttgttt
    16981 accgcctttg actttcttct cgaccagata tccgtcacat tttggacaat ttctgcccac
    17041 tggtttatcc caagaagtga attcacattc tgggaaacgg gaacaaccat agaatagtct
    17101 gtttttctta gattttcgtt caatgacttg tccttcatga catactgggc aagtcacacc
    17161 gatttctttt acgattggtt tcgtattccg acaatcagga aaattactgc aagcataaaa
    17221 cttcccatat ttccctaatt ttatcaccat cggatgtcca cagacatcac aatcgaatcc
    17281 tgctggttca tctttgattt gaattttttc gattttttct tccgcattcg tcagttcttt
    17341 ttcaaacggt ttatagaaac gatcaaccac tttgacccac gcttccgtgc cttcttcaat
    17401 tttatccaaa tctccttcca tttctgctgt gaaatggata tcgacgattt gagggaagaa
    17461 ttcacagatt aatgaattga caatttctcc tagttcagtc ggctcaaatc ttttttgtgt
    17521 caattttaca tagtagcgtc tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg
    17581 ccctaccccg ttttcttcta gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg
    17641 ttgagtaaaa tgttgttttg gttcaatatc aacagattgg actacatcgc cttcttcaag
    17701 ttctggaaga atattttctt tctcttcttt gccgtcatct ctaccttcca catacacttg
    17761 catgaatcct ttgaatttga tttttgaacc gttcgcaatg aagatgacac cattttgttc
    17821 taatgtcact ttcattgtat ctaaaatagc tggtgtcatc tgactagcca caaaacgtga
    17881 ccagatcaat gtataaagtt tcaattgatc tttatctaaa tattgtttga gttcatctgg
    17941 tgtacgaagc acactcgttg gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt
    18001 tttgatcttt cggtgatcat gtctagaaaa ttcttttcca tacgtatctt caataaatgc
    18061 tgcggcctct gcttttgctg tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat
    18121 caagccgact gttccttgtt tacccaaagt gattccttca tataattgct gtgctaccat
    18181 catggttttt cttgttcgaa agtttaactt acgggcagct tcttgttgca ggctacttgt
    18241 agtgaaagga ttcgcagggt ttcttttgcg ctctttcttt tctacttttt taacttcgaa
    18301 gtctgttcca tcaattctag cagtgacttc ttttacactt tgtgcatctg gtaatttctt
    18361 tttcttaccg tcgattcccc agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt
    18421 accgtcgatg ctccaatatt cttctggaac aaattctcgg atctcttttt cacggtcaat
    18481 aataattttt aaagcgacag attggacacg tcctgcactt aaccctttct tgaccttacg
    18541 ccagagaata ggactgatcg aataaccaac gattctgtct aaggtacgac gagcttgctg
    18601 tgcatctact aaatctacat cgatcgttct tggttcctta aaagctgctt tgactgcgtc
    18661 ttttgtgatt tcattgaaga ccacccgatt tttttctttc agatccaacc caagtaggta
    18721 cgctaaatgc caagcaatcg cttctccttc tctatccggg tcacttgcca agtaaacttt
    18781 ttgagctttt tttgctgcgg ctttcaggct tttgatgaca tcgccttttc cgcgtataga
    18841 aatataatgg ggttcataat tgttttcaaa atcgataccc attttacttt tcggtaaatc
    18901 tcttatatgg ccaacactgg ccattacttt atagtttcgt ccaagatatt tctcaattgt
    18961 cttggctttt gcaggtgatt ctacaatcac taaatattta tacgccatta ctgaatggac
    19021 tcctttccat tgtcgtgttc ctactattaa aagaggattc atacaaatcg tagctaatca
    19081 tatccattca gaaaacgttt gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa
    19141 attctgccaa gatatcttca cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat
    19201 gacatccggt agaatgttct gttaaaatat ttcctggaac agaaaaaact gttcttccaa
    19261 gatccaatgc ttgctgtgct gttatcagag agccgctttt tttcttggct tcaaagacac
    19321 atgttccttt agacaaagct gcgataatcc gatttctcat tggaaaacaa tgctttttcg
    19381 ggccggattg aggaggatat tcactcaaaa gaagatgatc atttgcaatc acctcttgca
    19441 agtcacgatt ttcagcagga tagacttgct ctatccctgt tccaatgact gcgattgtcc
    19501 gtccaccatg agagattgct gcttggtgac cgtatgtatc tatccctttc gcgcagccac
    19561 tgacgatcac atatccagct tctatcactg gttttagtaa ataataaact gtttttttgg
    19621 ctaaagcaga agtgtgtctt ccaccaacaa cgcttaacat gttacaagaa agtaaagata
    19681 gatcaccatt ataaaacagt acaattggcg gataggtcaa gtgaagcagc tgagggggat
    19741 atttgggatc aaagcaagtg ataaacgact gtttcgattg gattttgccc cagttagcgt
    19801 gaatttcctg ccaacttttg cgaaacagct cccggtgttt ggttatttta cctatcttaa
    19861 tgatgtcttc aaatccgatt tctttataag gatactgcat catccagtta gcaagtgccc
    19921 attttccttg actactgatt ccttttgtat aagcaagttt caataaattt tcttctattt
    19981 gatacatcat aaaaccgcct ttcgtctctc tttctaataa gatacgtaaa agcggctgtt
    20041 ttttattgta aatatttttt tacaggtgca aaacttttac gatggattgg cgtaattcct
    20101 tgtcgttcaa gtccttctaa gtgttccttt gttccataac ctgcattatg cgagaaacca
    20161 tatccaggat aaagcacgtc atattctttc atcaattggt ctcgatatac tttagcaatg
    20221 atacttgcag cagcaatcga cacagatcgt gcatcccctt tgattatttt ttcctgctga
    20281 ataggcaaat ccaaggtcat cgcatcgatc aacaaatagc ttggtacaat ggacaatttt
    20341 tctactgctt ggatcatcgc taatttactt gcttcataaa tattcacatc atcgattgtc
    20401 tgctgatcaa cgatcccgat cccgattgcc agggcttttt cttgaacttg ttgataaaga
    20461 agttctcgct ttttctctga taattgtttg gagtcattca gccctaatat gatctcattt
    20521 ttcggcaaga taactgcagc tgccacgaca ggtccagcta atggtcctct tcctacttca
    20581 tcaatgccgg caatccattg gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt
    20641 tcttcatatt tttccttaat taatgcttcc tttttcaatc gtcgttcaaa agaagccagt
    20701 gcttgctgca cgcctttgcg tggatcattt cttaatgatt ctagtgtagg atcatctatt
    20761 ctctgtatgc ttttcaatcg ttctttgata ctttggatgg attcagtcat cacttttttg
    20821 ctccatttct tcgcaacgat ccagtgtgta tcgtccaagt tttccttgac gaatatcatg
    20881 cacgactaat tcgctgccac gatcatagtc ttctttatac ccacgttttg ctgtaatatc
    20941 catcagtaat tccggtgcca atttttcttc ttgctcgatt gataaaccat aacgatcgct
    21001 gattcttcct gaataatatc gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa
    21061 atggacaagc tggtctttga tcgcgcctgt cagcgctagt tttttgccga tttcttcatc
    21121 ttcaaatttg ggccataaaa tccctggtgt gtctaacaat tccaactcat tacctaaacg
    21181 aagccactgc tgaccttttg ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc
    21241 tactagtcga ttcatcaacg tagatttccc aacatttgga atacctaaga ccattgcccg
    21301 aatagcgcga ggcttgatcc cccgagcagc atctcttgcc cgtttttctt tcaatgcttc
    21361 tttagccgca ggtactagac ctttaattcc tttactttct tgtgccgtga tagctaatgc
    21421 aagatggcct tgtttacgaa aatattcttg ccacagcttt gtctgttgtg gatctgccag
    21481 atccgcttta ttcaataaaa caaggcgagg tttttgctga atgatctgat ctagcattgg
    21541 attacgagaa gataaaggta atcgtgcgtc taccaattca aatacgatgt ccacatattt
    21601 cattttttca gaaacttctc gtcgggcttt tgccatatgt cccggaaacc attggatggt
    21661 cattgttctc tccattcctt tcataccgga atattccccg gctttacttg tttttttctt
    21721 tttcaatata aattcacttt tttattataa agaaattcca tctattagtt aagtgaaagt
    21781 gaaccagtcc tcattctatc acatcttcac ttttttgatg atgcaaattt ttattttggt
    21841 ttctatatat gaatctaaat ttttgacagc gtttcctaaa taagagatga tagacgaaca
    21901 aaacagaaag gagacgaaac aaatgaatag tatggttttt gttaattttc ctgttgctga
    21961 tgtcaatcgt tccgttgctt tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc
    22021 tactgaagaa gctggttcaa tggtatggga tgacactttt tggataatgc ttctctctca
    22081 tgatttttat aaaaaattta taaaagataa agaaatagct gataataaaa aaacaagtgg
    22141 tgctttgatt tcgtttagtg ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc
    22201 aaaagaaaat ggtggtactt tccacgaagt agatatggga atgcctgaag aagaaatgtt
    22261 ttcattagaa gtccaagatc tagacggaaa tacattagaa cccgtatgga tgaagatgta
    22321 agcagataac taatctgacg ttttttataa caaaaaaaca aaggcggatg atacgataag
    22381 tattctccgc ctctattttt tttattcgta caatctcttt tttacagttt ataaaagcag
    22441 tcttctcacc ctttttttaa ttgttagatg ccagtgtatg taaatcacca atgtgtcatg
    22501 acacattggt gattttatgg tatcctttta ttattaactg acaaaggaga gaatattttg
    22561 aacactttga ttgcaaaaga cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg
    22621 acctctgctc ttttattagc ccttgatgtc atcttataca agatagcgat tggtcctgct
    22681 tattttcaaa tcacattcgg atttttaagt atggcgctta tgggttatct atatggaccg
    22741 atctgggctg gtttgcttga aatgttgtcg aacactttaa atttcacgat atttggttca
    22801 ggaacgtttc aagctgcttt tcttatcacg gctttccttg gtggatttat taatggctta
    22861 tttttatatc ataaaaagat aaacctgcct tccgtcatcg ctgcccaatt attgattatg
    22921 atcccagtca gcctttttat caattcttgg ctgatgtctg ttttgtttgg tgcagatttc
    22981 aaagtaattt ttctaggtag attcatcagg aatatgatcc aaatccctat tcaaataatg
    23041 gttgtttatt catttatcaa aactttagag gctagaaaag tgtttaaacg agctgtttga
    23101 ttttacgtac taaggtagta aactgatttt ggattaaaca ataaagcgtg cagtctcaac
    23161 agactgcacg ctttattgtt tctcactgga tccattttat ttcatcaagt ggataataga
    23221 caaatcttgc tttacctaga atatcagttt tattgatcgt gccaaacgaa cgactatctt
    23281 ttgaaactct tcgattatct ccaagtacaa aataattatc ttccggaagc ttggattgtc
    23341 ccgttagctc ttgtagtgta aagtccgtcg tataggatgc atttgcatga tctgatttca
    23401 ggtttttagt tagataaggt tccttaacta ctttcccatt gatcttcaat tgatcatttt
    23461 ggtagctgac attttctcct ggaagaccga ttactcgttt gatataaatc gtgccatctg
    23521 ctaattgaaa aacaacaata tcaaatcgtc tgatctccga aaatttttcc atcacaacca
    23581 tatcaccttg cttcaacacg ttttccattg aattgccttc cactggtacc gggatgaata
    23641 aaaatccccg caacatgaac gcaaggaata cagaaacaag taagtatttg aatacaagcc
    23701 agaagcgatt aatatagcgt tgtttttttg tcaatctcta t
//
LOCUS       DAYYKN010000033        23653 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000033 DAYYKN010000000
VERSION     DAYYKN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.033"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..601)
                     /locus_tag="ACHBB7_001092"
     CDS             complement(26..601)
                     /locus_tag="ACHBB7_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM9209057.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="ACHBB7_001093"
     CDS             complement(606..1580)
                     /locus_tag="ACHBB7_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFM9209058.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="ACHBB7_001094"
     CDS             1863..2831
                     /locus_tag="ACHBB7_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9209059.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="ACHBB7_001095"
     CDS             2928..3221
                     /locus_tag="ACHBB7_001095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9209060.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="ACHBB7_001096"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="ACHBB7_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM9209061.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="ACHBB7_001097"
     CDS             3678..4433
                     /locus_tag="ACHBB7_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9209062.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="ACHBB7_001098"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="ACHBB7_001098"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM9209063.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="ACHBB7_001099"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="ACHBB7_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM9209064.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="ACHBB7_001100"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="ACHBB7_001100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM9209065.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="ACHBB7_001101"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="ACHBB7_001101"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM9209066.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="ACHBB7_001102"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="ACHBB7_001102"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM9209067.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="ACHBB7_001103"
     CDS             9170..9712
                     /locus_tag="ACHBB7_001103"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM9209068.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /gene="atpA"
                     /locus_tag="ACHBB7_001104"
     CDS             9735..11291
                     /gene="atpA"
                     /locus_tag="ACHBB7_001104"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM9209069.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="ACHBB7_001105"
     CDS             11305..12207
                     /locus_tag="ACHBB7_001105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM9209070.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="ACHBB7_001106"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="ACHBB7_001106"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM9209071.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="ACHBB7_001107"
     CDS             13650..14072
                     /locus_tag="ACHBB7_001107"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM9209072.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="ACHBB7_001108"
     CDS             14541..14771
                     /locus_tag="ACHBB7_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM9209073.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="ACHBB7_001109"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="ACHBB7_001109"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9209074.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="ACHBB7_001110"
     CDS             16182..16358
                     /locus_tag="ACHBB7_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9209075.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="ACHBB7_001111"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="ACHBB7_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM9209076.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="ACHBB7_001112"
     CDS             16853..17320
                     /locus_tag="ACHBB7_001112"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9209077.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="ACHBB7_001113"
     CDS             17405..18289
                     /locus_tag="ACHBB7_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9209078.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="ACHBB7_001114"
     CDS             18403..18639
                     /locus_tag="ACHBB7_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM9209079.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="ACHBB7_001115"
     tRNA            18727..18807
                     /locus_tag="ACHBB7_001115"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="ACHBB7_001116"
     tRNA            18814..18885
                     /locus_tag="ACHBB7_001116"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="ACHBB7_001117"
     CDS             19011..19181
                     /locus_tag="ACHBB7_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209080.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="ACHBB7_001118"
     CDS             19401..20108
                     /locus_tag="ACHBB7_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9209081.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="ACHBB7_001119"
     CDS             complement(20167..20811)
                     /locus_tag="ACHBB7_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM9209082.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="ACHBB7_001120"
     CDS             20938..21225
                     /locus_tag="ACHBB7_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209083.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="ACHBB7_001121"
     CDS             21333..21653
                     /locus_tag="ACHBB7_001121"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9209084.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21734..23047
                     /locus_tag="ACHBB7_001122"
                     /pseudo
     CDS             21734..23047
                     /locus_tag="ACHBB7_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            23044..23463
                     /locus_tag="ACHBB7_001123"
     CDS             23044..23463
                     /locus_tag="ACHBB7_001123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209085.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKRLPGIYLLSNTFI"
BASE COUNT         7820 a         4125 c         5092 g         6616 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa aagattaccc ggcatatatt tattgtccaa tacctttatc
    23461 tgaaaaaagg tggctagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaaa
    23521 aattcctttt cagatgctgc tcaaaagaga gcaacatctg cttgctcaag caaaaaacac
    23581 gcgaaaggaa agattggaga tgcctcagcc ttttgagctt gccgtacctc tttctaagat
    23641 acaacataaa aaa
//
LOCUS       DAYYKN010000034        23474 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000034 DAYYKN010000000
VERSION     DAYYKN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.034"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..1753
                     /locus_tag="ACHBB7_001124"
     CDS             92..1753
                     /locus_tag="ACHBB7_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9209086.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1823..2170)
                     /locus_tag="ACHBB7_001125"
     CDS             complement(1823..2170)
                     /locus_tag="ACHBB7_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM9209087.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2254..3195
                     /gene="opp3b"
                     /locus_tag="ACHBB7_001126"
     CDS             2254..3195
                     /gene="opp3b"
                     /locus_tag="ACHBB7_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM9209088.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3196..4239
                     /locus_tag="ACHBB7_001127"
     CDS             3196..4239
                     /locus_tag="ACHBB7_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9209089.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4245..5315
                     /locus_tag="ACHBB7_001128"
     CDS             4245..5315
                     /locus_tag="ACHBB7_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209090.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5305..6258
                     /locus_tag="ACHBB7_001129"
     CDS             5305..6258
                     /locus_tag="ACHBB7_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209091.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6270..7280
                     /locus_tag="ACHBB7_001130"
     CDS             6270..7280
                     /locus_tag="ACHBB7_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM9209092.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7565..8386)
                     /locus_tag="ACHBB7_001131"
     CDS             complement(7565..8386)
                     /locus_tag="ACHBB7_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM9209093.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8483..8761)
                     /locus_tag="ACHBB7_001132"
     CDS             complement(8483..8761)
                     /locus_tag="ACHBB7_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209094.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9029..11038
                     /gene="metG"
                     /locus_tag="ACHBB7_001133"
     CDS             9029..11038
                     /gene="metG"
                     /locus_tag="ACHBB7_001133"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM9209095.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11517..12104)
                     /locus_tag="ACHBB7_001134"
     CDS             complement(11517..12104)
                     /locus_tag="ACHBB7_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209096.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12279..13601
                     /locus_tag="ACHBB7_001135"
     CDS             12279..13601
                     /locus_tag="ACHBB7_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9209097.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13756..14532
                     /locus_tag="ACHBB7_001136"
     CDS             13756..14532
                     /locus_tag="ACHBB7_001136"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM9209098.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14525..15094
                     /gene="rnmV"
                     /locus_tag="ACHBB7_001137"
     CDS             14525..15094
                     /gene="rnmV"
                     /locus_tag="ACHBB7_001137"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM9209099.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15087..15971
                     /gene="rsmA"
                     /locus_tag="ACHBB7_001138"
     CDS             15087..15971
                     /gene="rsmA"
                     /locus_tag="ACHBB7_001138"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM9209100.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16422..17051
                     /locus_tag="ACHBB7_001139"
     CDS             16422..17051
                     /locus_tag="ACHBB7_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209101.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17175..18284)
                     /locus_tag="ACHBB7_001140"
     CDS             complement(17175..18284)
                     /locus_tag="ACHBB7_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209102.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18493..18915
                     /gene="mgsA"
                     /locus_tag="ACHBB7_001141"
     CDS             18493..18915
                     /gene="mgsA"
                     /locus_tag="ACHBB7_001141"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM9209103.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19102..19200
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19238..19774
                     /locus_tag="ACHBB7_001142"
     CDS             19238..19774
                     /locus_tag="ACHBB7_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM9209104.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19814..21577)
                     /locus_tag="ACHBB7_001143"
     CDS             complement(19814..21577)
                     /locus_tag="ACHBB7_001143"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209105.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21580..23295)
                     /locus_tag="ACHBB7_001144"
     CDS             complement(21580..23295)
                     /locus_tag="ACHBB7_001144"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209106.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7564 a         4295 c         4775 g         6840 t
ORIGIN      
        1 gtgttgattt tttaataatt attcattaaa ctcttaaaca acttcagaaa atattaatta
       61 ttctgaaaat tccaataaag gggtacgtaa aatgaaaaag aaatttacat ttggtttggt
      121 tgcggtatgc ggccttgtac ttgctggatg ttatggcggc agttcagcca caagcagtag
      181 ttcagcaaaa agcggttcag cagatggagg aggcgtattt aacttagtcg tgcctcaaga
      241 aatgcctaca gctgacttat cagttgcaac agatacaatc agttttacag cattgaataa
      301 cgtttatgaa ggaatttacc gtcttgatga agacagcaaa ccacagccgg caggagctag
      361 cgagttggca gaagtcagtg aagacggatt aacttacaaa atcaaattac gagaagacgc
      421 aaaatggtca aatggtgatc ctgttacagc agcggattac gtttatggct ggcaacgaac
      481 agtagaccca gctacagctt ctgaatatgc ttacctgtat gcgccagttg aaaatgcaga
      541 agacattaca gctggaaaga aagataaatc agaactaggg atcaaagcag ttggtgacta
      601 tgaattagaa atcaaattga cgaaacaaac accatatttc caatatctgc ttgctttccc
      661 atcatttttc ccacaaaaac aatccgtagt ggaagaaaat ggggatgcct atgcatctgc
      721 aagtgataaa gctgtttata atggaccatt cacattagaa gattttgatg gaccaggaac
      781 agatacagaa tggacttata agaaaaatga tcattactgg gacaaagata cagtaaaact
      841 ttctgaaatc aaagtcagtg tagtaaaaga atcttctact gcattgaact tattcaaaga
      901 tggccaagca gatgacgtga tcttgtctgg tgagttagca cagcaaaacg caaacgatcc
      961 agcttatact tctgttaaag aagcgcggac aagctatatc gaattcaatc aacgtgaaaa
     1021 gaattcacca ttcaacaatg tgaacctaag aaaagctatt tcttattcta tcgatcgtga
     1081 agctttagta aaacaagtac taggtgacgg atcagttgtc tctacaggat tgatcccatc
     1141 agatatggta aaaagtccaa caacgaacga agactttgca aaagaagctg gaaaattagt
     1201 ttcttatgac aaagataaag caaaagaata ttgggaaaaa gcgaaaaaag aattgggcgt
     1261 tgattcttta gaatttgatt tgatggcttc tgatgatgat tcatcgaaaa aagtaatcga
     1321 atatgttcaa aactcaatcc aagaaaactt ggatggcgta acagtcaaac caacacctgt
     1381 tccattctct gtacgtcttg accgctcaac ttcgggtgat tttgacgcag tactaggcgg
     1441 atgggctgct gactatgcag acccaagcag ctttactgat ttgtttgtca caggtaattc
     1501 atacaaccgt ggtcgctgga gcaatgcaga atatgacgaa gcagttaaag cagcttcatc
     1561 aacagacgct ggcgatgaac aagcccgttg ggatgatttc caaaaagcaa tcaagatcat
     1621 ctctgatgat atgggtgtcg ttccggtgta ccaaaaagca gaaggtcacc tagtgaatga
     1681 aaaaatcaaa ggtatcgttc accatgcggc aggcgcttca tgggattaca aatggacata
     1741 cgttgaagaa taatcgttaa taccgtgagc atgagaaaaa acgaggctgc ggcaaatgtc
     1801 gcaacctcgt tctcatattc acttatccta acaaagaaaa aagcatgaaa atagcagaaa
     1861 gagcaattag tattccggca atgatcagcc aaaagctgta cataccgtag ctggcagacg
     1921 agagcgtgga aaattctggt tttttctttc ggttccttgt tcgtgcaaga tggaccgatc
     1981 gctgaaaatg atcgaagaaa cccgaagaaa agacccataa aaaaccgccg ataatcagaa
     2041 acggcaatga ccataagaat agatcattag ataattgaat gagtgtaaag gaatggttga
     2101 tgagattttt tattaaaata accagaatca gtagaaaagc tactgcgtag gggatagatt
     2161 tttttttcat ttgattgctc ctttattttt ggttcattat ctaataatgt agcgaaacat
     2221 ctgtcagaag tcaaaagcta gggggaaaca ttagtgaata gttatatcaa atatgtatta
     2281 aaacgtgtct ttttcatgat catcacgctt tggttaattg caacaatcac ctttttcttg
     2341 atgcaattgc tgccgggcac gccatatacg aatcaggaac gtttgagtcc tgaaacaatc
     2401 gagatgctaa ataaacaagt cggtttggat aaaccagtta tcgtacaata tgggatttat
     2461 ctttctaatt tgcttcaagg agattttgga atttccttcc aatttaaaaa ccagccagtc
     2521 gcacatttgt tagctggaag aatcggacca tccttgcagt tagggttaca ggcaattatc
     2581 tttggaacag tcctagggac gattttagga acgatctctg ccatgaagca aaatacatgg
     2641 gcagatacat cttctacact cgtagcgata ttaggtcgtt ccattccaaa ctttgtattt
     2701 gctgttttat tacaatatat ctttgcaatc aaattacaag ttttgccaat cgcaaaatgg
     2761 gatggctttg tgtacacaat cttaccaaca attgcgttgg ccatgtcgcc gcttgctgac
     2821 tctgctcggt ttatccgtac ggaaatggtg gaagtactgc atagtgatta tgtggaatta
     2881 gcaaaagcaa aaggattgag ccgttgggaa atcgccttta aacatggtct tcgaaatagt
     2941 ttgattccat tgatgacttt attaggaccg ttagctgtag cattgatgac cggatcactg
     3001 gtggtagaaa atatctttgc tattcctggt atcggggaac aattcgttaa gtcaatcaca
     3061 acaaatgatt atcctacgat catggctgta acgatccttt actcatttat gctgattttt
     3121 gttattttga tcgtggatct attgtacggt ctagttgatc caagaattcg tgtttcagaa
     3181 gggagccgag gataaatgga aatgatacca aagcgttatg aaacagttgc agatatccct
     3241 gcggatgaat ttcttccatt acaaaaaaat accgaagaag aacgtgaaca gattactgca
     3301 ccttctttga attttatcca agattcatgg cgccgtttaa agaaaaacaa agctgccgtt
     3361 atttcaatgg ttttactatt ggtcatcatt ttgatctctg tgatcacaat ctttgtttct
     3421 cctcatgatc caactgccca aaacgtggct tatatcaatt tgcctcctcg tattccggga
     3481 atcaatatcg atggattgaa tggaaaagca atggttggtg gtgaacttgt tgataaatat
     3541 gctcaagcaa acgtcccttc aaacgtaaat ttctttttag gaacagacgg tttgggacgt
     3601 gatgtattaa gccgcttatt tatgggaatg agaatctcgt tgctgatcgc atttattgct
     3661 gcattactag atgtaacgat tggtgtagct tacgggttgg tttctggttt gttaggcgga
     3721 cgtgtcgata acgtgatgca acgttttttg gaaatcttat caggaattcc aaacctcgtt
     3781 gtcatgattt tgatgttggt cgtttttgaa ccaggaattt tttcaatcgt ggctgctatg
     3841 gccatcacaa actggatacc aatggcacgt atcgttcgtg cacaaacaat gaaattaaaa
     3901 gatcaagaat atgttttagc tggattgaca ttaggtgaat caagaatgaa aattgcattc
     3961 aaacatattt tgccgaatat ctctagtgtc attattattc aaatgatgtt tagtattcca
     4021 tctgctattt tcttcgaagc ctttttaagc tttatcggtt taggtcttac gcctccgtca
     4081 gcttcattag gaacgatgtt gagcgaaggg tacaagacat tcctgtatct gccttatcta
     4141 ttatggattc cggcagtaac tttatcggtt atcatgattg gctttaactt acttgctgat
     4201 ggattgcgtg atgcctttga tcctaaaatg aaagagtgaa gacgatgacg aaaattttag
     4261 aagtgaaaga cttagaaatc tcttttgata cgtatgctgg aaaggtacga gcgattcgcg
     4321 gtgtcaattt tcatttgaat aaaggagaaa cattggcgat cgttggggaa tctggaagtg
     4381 gaaaatcagt gacgaccaga agcatcatgc gcttgctgtc aagcaacgcc aatatagatg
     4441 ctggtcaaat cctctttaaa ggtaacgata tcgttggaaa atcagaaaga gagatgcaaa
     4501 aaatccgtgg taaagaaata gcgatgattt tccaagatcc aatgacctct ttagatccga
     4561 cgatgcccat tgggaagcaa gtcgctgagt cccttagaaa gcataataaa gtttcaaaaa
     4621 aagagggaca aaaagctgca ttagaattgc ttaaattagt tggaataccc gaagctgaga
     4681 aacgtatcaa cagttatccg catcagttct caggaggtca gcgtcaacgg atcgtgatcg
     4741 cgattgcact ggtatgttat ccggaaatcc tgattgctga tgaacctaca acagcgcttg
     4801 atgtgactat tcaagcacag attttggagc tgctgaaaga aattcagaca aaaatcgata
     4861 catcaattat ctttatcact catgaccttg gtgtagtagc gaatgtcgct gatcgtgtcg
     4921 ctgtcatgta tggaggaaga atcgttgaag ttggaacatc ggaagaaatt ttctacaacc
     4981 cgcaacatcc ctatacttgg ggactgcttg gttcaatgcc gacattagat agtgcgaacg
     5041 atcgattgta tgctattcct ggttctccac cggatttatt gaacccgcca aaaggagacg
     5101 cattctatcc aagaaatgaa ttcgcgatga aaatcgatgc agaacaagaa ccaccatttt
     5161 ttgaactatc aaaaacacac aaggcagcaa cttggctact tgctccgcag gcacccaaag
     5221 tgacaccgcc agaagaaatc caacgtcgct gggctatctt taaagagaaa caaaaacata
     5281 cttacggtca aggaggaatt gctgatgcca aataaactac ttgaagttaa aggattgaaa
     5341 cagtatttca atgtaggaaa aaagaatgaa gtacatgctg taaatgacat cagctttcac
     5401 atttatgaag gagaaacatt cggtttagtt ggagaatctg gaagcggaaa atccactact
     5461 ggccgtacga tcattcgctt gaatgaacct actgatggtg agatattatt cgacggtcaa
     5521 gatataacga agatcaaaga taaaaaaggt ttgacgaaat tccggcatga tgttcagatg
     5581 atctttcaag atccatacgc ttcgttgaat cctcgtatga aagtcagaga tatcattgcc
     5641 gaaggaattg atgttaatgg actagctaaa tcatctgaag agcgtgcaaa aaaagtcgat
     5701 gatctattga gaactgttgg attgaatcca agccacggta ctcgttatcc tcatgaattt
     5761 tcaggtgggc aacgccagcg tattggtatt gcacgagcat tggcagtaaa accgcgtttt
     5821 atcatttgtg atgaaccaat ttctgcacta gatgtgtcga tccaagccca agtggtcaat
     5881 ttgttgcaag atctccaaaa agaacatcaa ttgacctatc tatttattgc acatgatttg
     5941 tcgatggtta aacatattag tgaccgaatc ggtgtaatgc ataacggctt gttattagag
     6001 atgggaacaa gtgatgaaat ctataatcac ggtgttcatc cttatacaga aagtttgtta
     6061 tctgctattc ctctaccaga tccagaccat gagagaaaac gcagaagaat caaatatcaa
     6121 cctgaaccag atgacggaca agtacgtcaa cttcgtgaaa tcgcaccaga acattttgtc
     6181 tatgcaactg agcaggaagt cccatattac gcaaaaaaac taaaacgaca aaaggaatct
     6241 cttctggttg ctaactagga gggacataga tgctaaaagc ttttaaattc cgtatgtatc
     6301 cgacagaaga acaaaaacag caattgattc gtacttttgg atgtgctcga tttacctata
     6361 accatttgct gaaaaagcgg cagaaaagtt ggcaacaaac aggagtagcc aatttttcgc
     6421 tcacacccgc tacattaaaa aaagaatacc cattcttaaa agaagtagac agtcttgctt
     6481 tagcaaatgc acagcttaat ttggatcggg cttttcgcaa ttatttcaag ggacgtgcca
     6541 gcttcccaaa gcttaaaacg aaaaaaagta tgtggcaatc ttatacgacc aacaaccaaa
     6601 cccgtacgat ctatctggaa aatggctact taaagttacc aaaacaaaaa gaactaatca
     6661 agataaatca gcatcggcca gttgaaggat cgattcgctc cgctacgatt tctgctcgat
     6721 ataatgaaga attttatgtc gcactacttt gtgatgtttc gccagtaaaa aaagaatctt
     6781 tagctaaatg gatcgggatc gcctatcatc cgaagacatt gatccaaaca tctcgacctc
     6841 tagaagtaac tttaccaaaa tttcaccaga cagaagaaaa gctgcaacat gcacagcgga
     6901 aattgaatgt gaaagtccgc tccgctcacc atagaaagat caggttagat caggcaagca
     6961 attatcagaa acaaaagaga aaagtaatgg atctttatct gaaacaaaag aaccagcgag
     7021 aagattatct tgaacaatta tctgggaaat tggtcaaaca atatgactac ttatttgtag
     7081 aatcttttcc aaaagaagag gcacatgctg atttttctat tcacgactgg cataaattaa
     7141 taacaaaatt gcgttataag tcccaatggt ataataaaaa atttcttttg atcaataccg
     7201 acggagcaga agaaagcaat tccgtaagaa agagccaagt agtagaaaaa atgggcagac
     7261 attcggttat caagggataa aaaatgaatg aaactgatag gagcgttagg gttagccttg
     7321 ttaataaaaa gtcttaatga cttttgtttc taggaatttt tcttttgaag aaaagctcac
     7381 taattatgac aaaccaataa aacaatagca gaaaaagttc gtacgagctt tttctgctat
     7441 tgttaatttt ttattcagaa agtttacgaa tacatcaaaa ttctgaaaca aaaaacgtaa
     7501 ctcacaaaga gttgtttttg cgttttttct tgaatgatta ggtctgaatc ttttgctcac
     7561 aaccttattc atggtgcccc ggcttgagct gatagatttt tgaaggacgg ccgattccta
     7621 caggacgttc accgatctca tcgaaatatt ggagcatagc tttcttaaaa ttggaatgat
     7681 cgatcgtttt ataatcgatc cctaagaatt tagcaaatac ttttcgagct tccgtaatcg
     7741 tgaaatcttt acctaaaact tgtagaactt gcggctcgtg ttccatttta ttgactacac
     7801 ggttgaatgc tttcaagatg atttgactat ggtcaaaagc caaagtatct ttgccagaag
     7861 actctccagt gactaaatca agcgagatag ccacttctcc gctagataaa tggatcttgt
     7921 tactatgacg ttctagtgtg aaccagcgta cttccttggc atcatctccg gcgattagcg
     7981 gttcttctcc aataaaggct aagtagctga ctgtgacgac ccagcctcgc ggatctcgat
     8041 taggcgtact gaatgtatgc agttgctcaa tattttcttt tgaaataacc acaccagttt
     8101 cttcctttgt ttctcttaag acgctttctc cagttgactc atccttttgg acaaagccac
     8161 caggcaatgc ccatgaattt cgataaggat gcccttttct ttggatcaac aaaactttca
     8221 gctgatcttc ttctttgtta tagcacatca gtacgatatc gacagtcaaa gagggcttgt
     8281 catattctgg acgttcctgt ttttgatacc agttaagaaa ttcttcttca cttgctttat
     8341 gttcataata taatttttct tcttctttag aagcaaaacg gttcatctga ccacctccat
     8401 tgacagtaaa agtacttttt accttattgt agcagactct gaaaaaaaac caaggattta
     8461 cttgaaaaat ctaggttatc atttaaatat gaaaaataaa tggaagtatg agattgaata
     8521 aaaagaaaag cagataaaac ggcaatactt tataccgctc ttcacgaatg gcaaagaagc
     8581 cacaaataac gatatatacg tcaatgataa aacgcaaaat ccaagctgct gtcataaaac
     8641 tgttcaaatg cccatggagg ttaaaataac catacatacg aatataaaaa tagctgaata
     8701 ttaaatgtgc taatgaaaga aaagcaatac taagtgtaaa aaaaagatgt ttcttctgca
     8761 tgattgttcc agtcccttac taagatagat agtctttatt ataccacgtg tgaggggaga
     8821 aataaaaagc cgttttttag tagatatgaa acaaaacaaa aacccgcatt tattcaacct
     8881 attcatcctt ttaaaggata gattgaacgt agatagaagt cgtttatcca gtctttttca
     8941 ttttgaaaaa aagctaacag catgcttttt ttatggtata ctagcaagga aacgaattcg
     9001 catcatgcga taaaggagga agagccccgt ggcagaaaag gaaacttttt atatcactac
     9061 gccaatctat tatccaagcg gcaaacttca tatcggcaac tcgtatacta cgattgcatg
     9121 tgacgcaatt gctcgttata aacggttgat gggctttgat gtgttttatc tcactggagt
     9181 ggatgaacat ggtcaaaaaa tcgaaacaaa agcagaagaa ctaggtgtaa aaccgcaaga
     9241 atatgtagat aaaatggcgg ctgatgtcaa gaaattatgg aaaacgcttg atatcagtta
     9301 tgataaattc attcgcacga cagatgatta ccataaagca gctgttcaaa aaatatttga
     9361 tcgtttatta gaacagggag atatttactt aggtgaatat gaaggctggt actctgtttc
     9421 tgacgaagaa ttctttacag aaacacaact tgcagaagtg tacaaagatg acgaaggtaa
     9481 cgtgatcggt ggtaaagcgc caagtggaca cgaagtagaa ttggtaaaag aagaatcata
     9541 cttcttccgc atgagcaaat acgcagaccg tctattggaa tactatgaag aacatcccga
     9601 attcatccag ccagaatctc gtaaaaacga aatgatcaac aactttatca aaccaggatt
     9661 agaggatctt gcagtttcac gaacaacttt tacttgggga gtaccagtca agagtgatcc
     9721 gaaacatgtg gtgtatgtat ggatcgatgc attatccaat tatattactg cactaggata
     9781 tggctccgaa gatgaaagtc tattcgaaaa atactggcct gcagatgttc agatggtcgg
     9841 taaagaaatc gttcgctttc atacgatcta ttggccaatc atgctgatgg cccttgattt
     9901 gcctctacct aaaaaagtat ttgggcatgg ctggctattg atgaaagacg gaaaaatgtc
     9961 taaatcaaaa ggaaatgtcg tttatcctga aatgcttgtg gaacgttatg gtttagatgc
    10021 actccgttat tatttgcttc gtgctgttcc gtttgggtca gatggtgtct ttacgccaga
    10081 agattttgtt tcccgtgtca attatgatct ggcaaatgat ctagggaact tattgaatcg
    10141 aacgattgct atgatcaaca aatattgtga tggactcgtt cctgactatg cctcacttgt
    10201 cactccattt gatagtgaac tgtctacgac ggctgctaac gttgtcggac gttaccatga
    10261 tgcaatggaa aaaatggaat tcaatacagc attggctgaa atctgggtgc tgatttctcg
    10321 tgcaaacaaa tacattgatg aaacagaacc atgggtatta gcaaaagacg aagaaaagaa
    10381 agctgaattg aatagtgtca tgatccattt agctgaatct ttgcgtatcg cagcgatatt
    10441 acttcagcca atcatgacag aaacgccaga gaagatcttc aatcaactag gattggactc
    10501 tgaaacaatg aatcttgaag gattacactt tggcgaattt ccttcaggaa caaaagtagt
    10561 tgcaaaaggt acaccaatct tcccacgttt agatatggaa gaagaagttg cttttattca
    10621 agaaaaaatg tctgaaggta ctcaaacaaa cgaagataca gtcaaatggg atccagaaga
    10681 aacagaactt gtttcaacga aagaaaaaca aatcaaattt gatgtatttg aaaaagtaga
    10741 actaaaagta gcagaagtca tcaactgtca aaaagtagaa ggggcagata agttgctgca
    10801 attccgacta gatgctggag acagccaaga ccgtcaaatc ttgtcaggaa tcgctgaatt
    10861 ttatcctgat ccaagcgaat tgatcgggaa aaaagtcgtc attgtagcaa atctgaaacc
    10921 acgaaagatg cgtggacaaa tcagtcaagg aatgatcctt tctgcagaag caccagatgg
    10981 ctctttacaa gtaatcgaag caccaaaatc catgccaaat ggctcagaaa tcgcatagaa
    11041 attcaaaggg ggtatgacac tagttgtgtc ataccccctt tttccgaaat ttgagaagca
    11101 attttcgtaa attccttctt attacttgaa gccaatcata taatacagaa acagagaact
    11161 atccaaaata aatcctagaa aaagaagtac gagcttcgct gatttttgtt aatttagaat
    11221 aatactaata ggaatcgtaa aaacagaaaa aaagcataat gataaagggt cgggcagatt
    11281 ttaattgatt catctggtac tcttttttat tgtccttaca ttcataaagt ttgaatgcaa
    11341 tgactttatt acatctatat ttatatctaa cagtagcgtt gatgtgttaa ttataagttc
    11401 tatcacttta tgttcaatta gtttgtgctc ttatatgtgt gattgaaaat atagataaaa
    11461 aaacaatcat cggctttgac ttcgatgatt gttttcttta ttttgtaaaa atcctatcac
    11521 ttgattttat ggtaaccttt tatttttgtc ttcaaacggc ttggtaagag agcagtaagc
    11581 agtgcaaatg ggatcgaaaa gacataccag aaaatcgtac gtccgatacc ataatgttga
    11641 tagagctttg tcaacatcac gtgtaacaag atcaacccta aaagcaagga gccagctata
    11701 aaccaatgat tatcccaatg catgacttca ctaagtgcca tcactccaaa aggatagaaa
    11761 taaaaataga tcccgactaa ggtcatccgc cagttctttg taaatgttcc gatattcaat
    11821 aaaaagaaaa tcgctgaaag caaaataccg ataggcggaa aaagcccaac caaaaaggaa
    11881 tacaacatcc cccacaagat catcacagga cctttgatca atgcaacgat tgcgatgaac
    11941 aaagtcagca atattagttt ttgtgataac gttgcaacag tcattaatcc aaataaaatt
    12001 aatgtgaacc aatgccaatg ggactgctgt tgttctttac gggaaaaatc gacccggttg
    12061 gataaataat ctgctaattt cggataagaa cgcttaaaat ccataaactg ttcctccttc
    12121 gtggaaaagg taggtttagt atacgaaaaa taaaaaagaa atacatccgt cttaagaggg
    12181 agtttttcac tgaaagtgga gaaaaaagaa tggtaaatta aaatattctc agttatactg
    12241 aaaattatac caaatttcaa agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag
    12301 aaatcaatct gtttggcgca ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct
    12361 tcttcaaaac tgctgctgtt actgctagta ctcagtctac aagtttgaca ttgctagctt
    12421 ggcttttggg tggtttctta acgatctgtg ctggattgac agtggctgaa ttagctacag
    12481 caatcccgga aactggtgga gctgtaaaat atattgaagc tgcgtatgga aaattgccca
    12541 gctttttatt aggatgggcg caaagcttga tttactttcc tgctaatatt gctgcattat
    12601 cgattatctt tgcgacacaa ttgacgaact tattacaatt atctaccgat tatcttttgt
    12661 tgattgcagt gatcaccgct gtttctgtga cagggttgaa tttgttaggg acaaaagtcg
    12721 gtgcttctgt acaatcagtg acgttgattg ttaaattaat accaattgct gtgattgtca
    12781 tatggggatt attgacccca ggacaaggaa ctgtccagtt atttccaatc gaagctggaa
    12841 aagatgttac ttttgtcgaa ggattgagca gtgcgttgtt agcaacgttg tttgcttatg
    12901 acggctggtt aggtgtcggt gcaatggcag gggaaatgaa acggccggaa aaagatttgc
    12961 ctaaagcaat tattcttggt ttgagttttg taacagtcgt ttatctattg atcaattttg
    13021 tttttttgaa aactttgccg attgatcatc ttgccggtaa tttgaatgca gcttctgaag
    13081 catcagacgt catttttgga ggaatcggtg gaaagctggt cactattggg atcttgatct
    13141 cggtatacgg tgcattgaac ggttatacac taactgggat ccgcgtgcct tatgcgatgg
    13201 ctttagaaga cgagctacct ttcagtaaac agctgacaaa gctttcaaag aagtttacgg
    13261 ttccttatgt tcctgcggtt ttccagttag cagtagcttg tattatgatg agccttggtt
    13321 ctttcgactt tttaacagac atgttgattt ttgtgatgtg gctattcagt ttactgattt
    13381 ttatcggtgt ctttatttta agaaaaaaag cgccggaatt gccacgtcca tataaagtac
    13441 cactttatcc aatcgttccg attattgcga ttctcggtgc tattttcatt ttaggtatga
    13501 cgatgatgac gcaaacgaaa ttagcattga tcggtatagg tgttaccttg ataggtatcc
    13561 cagtctatta tcaaaaaaag aaaaaacgct cagaagagta aaatatttca acaacagaca
    13621 gaaaaagtac gaactaggtc ctctagatct tcgtgctttt tctatttcaa taaaacaagt
    13681 tcttctattt tgatttttgc tacttctgca agtagtgctt atgtgctaaa gtaagaatga
    13741 aaaggagaga taaacatgat atttgattct catacccatt taaatgctga acagttcaat
    13801 gaagatattc cagaaacgat tcagcgagca caagaaatgg gggtaacgaa aatggcggtc
    13861 gtgggcttcg atacgccaac gattgaaaag tctttgcaat taagccatga ctatccaaat
    13921 atatacagta ttatcggctg gcatccgaca gaggctggca gttatacgaa agaaatcgaa
    13981 aataaattac aagaacaatt gactttacca aaggttgtag cattagggga aattggcttg
    14041 gattatcatt ggatggaaga cccgaaagaa gtccaagata aagtatttcg cagacagatt
    14101 gctatcgcaa aagaaatgaa cctaccaatc agtattcata ctagagaagc acttgaagac
    14161 acgtatcaaa tcctaaaaga agaagatgtt cgagatatcg gtggaatcat gcatagtttc
    14221 agtggtgatt atgaatgggc aaaacgattt cttgacttag ggatgcatat ttcatttagc
    14281 ggtgtcgtca ctttcaaaaa agcattagac gtccaagagg ctgcagcaaa tgtaccaatc
    14341 gaacgattac ttgtggaaac agatgcccct tatctggcac cagttccttt tagaggaaag
    14401 cgaaatgaac caggctatac tcgttataca gtagaaaaga ttgctgaact tcggaatctt
    14461 ccgacagaag aagtggctgc ccaaacatgg acaaatgcgc accgattatt tggtctaagc
    14521 gaacatggat aagatgaaga tccaagagat tatagtcgtt gaaggaaaag acgatacaag
    14581 aaggctgcgg gaagtagttg atgcagatac gatagaaaca atagggtctg caatcaatga
    14641 agagatcctg atgcaaattg aacatgccca agagactcgt ggagtgatcg tttttaccga
    14701 tcctgatttt tcaggagaaa aaataagaaa aatcattatg gaagctgttc ctcaggcaaa
    14761 acatgctttt ttgccgcgca cacaagctaa accaaagaaa aagggaagcc ttggtgtcga
    14821 gcatgcaagt gatgctgcta ttttagaagc attgaaaaag gtcgtgacgc cagacatttc
    14881 ccaaacggct gtttctgaaa tcactagaca agacctttta gactatggtt taatagctgg
    14941 ggcacaagca aaagaaaaac gagaaaaact aggtgacgag ttgcgcatcg gttacacgaa
    15001 cggcaaacaa ttggaaaaac gtttgaagat gtttcgtatt acgccgaaag aactcgccca
    15061 agcaatgaaa atagtggagg aacaacatga gtgaatatcg tgaaattgcg acaccctcta
    15121 gaacaaaaga gattttattg aaacatggtt tttcatttaa aaaaagttta ggacaaaatt
    15181 ttctgactga accaaatatt ttaaggaaaa tcgtagaaac tgcgcaaata gatgaacata
    15241 cgaacgtgat cgaagttgga ccaggaatcg gtgcattgac ggaacaatta gccaagcatg
    15301 caaaacaagt cgtagcattt gagatcgatg atcgtttgat ccctgtattg gctgatacga
    15361 tgcaacccta tgacaatgtg actattgtac atcaagatat tttaaagacc gatttgtcaa
    15421 cagctgttag agaaacattt catgaggagc ttccgttaaa agtcgtagcg aatttacctt
    15481 actatattac gacaccgatc atgatgcatt ttcttgaatc agatttggtt gtggatgaac
    15541 tagtcgtcat gatgcaaaaa gaagtagctg atcgtatctc tgctgaacca ggaacgaaag
    15601 cttatggttc attatcgatt gctgttcaat attatatgga agccagttta gcattcatcg
    15661 taccaaaaac agtatttgtc cctcagccaa atgtagattc agcgattttg aaactgacaa
    15721 gaagagatat accggcagtc gaagtgacag acgaaaaagc atttttccga ttgacaaaag
    15781 cggcatttca gcaaagaaga aaaacgcttt ggaataactt gcagcattcg tatggcaaag
    15841 atgaccagac aaaagcatgg ttagcaaaaa gcttggaaac agctggaatc gatccaaaac
    15901 gtcgtgggga gactttgtca ttacaagaat tcgccgcatt aagcaatgcg atgtcagaaa
    15961 atatgcagta aaaaaagagg ttgtgacaga agttgtgaaa aagctgtcct gcatcaagta
    16021 ataagaacag ccaaacaaaa atagttcctc atgtttttcg tttgtttggg cttattaccg
    16081 cagatgcaag cttttgaaca ccgtttaaga aagaggttgt gggcctgccg tttaactcta
    16141 agtcacaagg aacaatttca aaaaacagct tctcatgttt ttgagttagt tggtcttatt
    16201 acttgatgca agcttttgac cactattatg ccgaacaagg agatgtgaca caaattttgt
    16261 cacatctcct atttttgttt cttgatcaaa aaattcttat acttgatact cacactcatt
    16321 ttgtatggat ttagaaaaga atatgtgtct tgacagttat tagtaagtga gttatactaa
    16381 gtcgacattt atgtgtcaag acatattagg agagagaaaa aatgagaaaa aattcagttc
    16441 gaaaactaac gatttctgct ttattgattg ggatgggggt cattatccca atggtcatgc
    16501 ctaaaatcat gattccacca gcttccttta ctttggcgtc tcatgtgcca ttatttattg
    16561 cgatgttttt tacaccagga gttgcagtgg ctgttgcgct aggtacgaca tttggtttct
    16621 ttttaacaac gccggttatc atcgcattgc gagcattgtc acatcttgtg tttgcgttga
    16681 ttggggcttg gtacttgcaa aagcatccga gtatcgtttt aaaaaatgga cagtttactt
    16741 ttgctaattg gcgtttccaa gggttcaatt ttgtgatcgg tgtgattcat tctttagcag
    16801 aaatgctagt agtcagcatc ttttacttta ttggtaatat gcctgaaaca tattattctc
    16861 aaggatattt ttatacagta ttcatcttga tggggattgg tgggcttatc cacagtttag
    16921 ttgattataa tattgcttat tttatagcag gaacactcag caaatatttt gatatccccg
    16981 tatttacggc agcaaaaaaa gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa
    17041 cgacatatta gacatgatct ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc
    17101 ctctaagatg agagtttttt gtcctaaaaa gagctgcacc aacatcttct ggcacagctc
    17161 taacaggggg caatttatgg aagtttgatg acttgttctg tttctttatc aaagaagtgg
    17221 cctttgctga tgttgaaggc aaggtcgact atttcgcctg gtttatggaa atctcttgca
    17281 tctacttttg agatgaactc agtagaacca gtttttgtgt aaagcatcgt ttcagctcca
    17341 agtaattcgg aaacgacaac ttctgatttc actacggctt caggtgaagc ttcaagggct
    17401 acttgttcac tgtggatgtc ttctggacga ataccgaaga taacttcttt tccttcataa
    17461 cctttttcaa ctaatacttt gttttttcct tcaggaatac gaagcttaag cccatgtcca
    17521 tctgtgataa caccattttt caatgtcaca ttaaagaagt tcatggcagg agatccaata
    17581 aagccggcaa cgaacatatt gacgggtgta tcataaactt ctttcggtgt accgatttgt
    17641 tggataaatc cgtctttcat gataacgatt cggtcagcca tagtcattgc ttctgtctga
    17701 tcgtgagtaa catagatggt cgttgtttca agacgctggt gaagtttggc aatctcagca
    17761 cgcatggcta cacgtaattt agcatctaaa tttgataatg gctcatccat taagaagact
    17821 tttgcatccc gaacgattgc ccgtcccaat gccacacgtt ggcgttgtcc accagataaa
    17881 gcagctggtt ttcgtttcaa gtattctgtc agacctaaga tctctgctgc attgtccaca
    17941 cgttttttga tgtctgcttt atcatattta cgtaatttca agccaaaagc catattatca
    18001 aaaacagtca tatgaggata caatgcgtag ttttggaaaa ccatggcaat atcacggtct
    18061 ttaggtgcca cgtcattcat gacttttccg ccgatattta gttctccttc agagatatct
    18121 tccagtcccg cgatcattcg caaagtagtt gactttccac aaccggaagg accaacaaaa
    18181 acgataaatt cacggtcttt gattgataga ttaaaatcag ttacagaata attttctgca
    18241 ttgtcatatt ttttatatac gtttttcaga gccatttcta ccataatacg ttccacactt
    18301 tcttttttag ttttattaac tatttaaagt ataaatgaaa gcgttaacta acttcaatgt
    18361 catgttgaac aagaattttt tctttttttt gtcaatgtgc tagttttaag ctttaataaa
    18421 tatccataag aaataaaaac tgatacactg gagtaaataa gtatgttaac aatatcaaaa
    18481 ggagtgtctc ttatgaaaat cgctttgatc gctcatgacc gaaaaaaacc tatgatggtg
    18541 aaattagcaa cagcgtataa aacaatattg gaaaaacatg aactttttgc taccgggacg
    18601 actgggcaga ggatcacgga agcaaccggc ttgccgatcc atcggtttaa atccggaccg
    18661 ttaggcgggg atcagcaaat tggagccatg atttcggagg atcagttaga tttggtgatt
    18721 tttcttcgag acccgctggc tgcacagccc catgaaccag atgtaacagc acttatccgt
    18781 ttatctgatg tctatgagat tccgttagct acgaatatcg gaacagctga ggtgctacta
    18841 agaggactgg aagccggctt cctagatttt cgaagaatcg tccatgaaat agacaagcat
    18901 ccactttcat tttgatggat aagcgtatgg aaaaataacg tttaatgttc gcctatatgt
    18961 agctataaaa gctaaacaaa ctgataatat gcgatataat acgaacgtat tggataatat
    19021 aaacaataat attagttttt cgtatttgca gtaaatgtga aaatatgcta tagtttctgt
    19081 gggaaaagat cccaaaaata atacagagta gaaaataaag cgttaagtgc taagaggatg
    19141 ggatgttgcc tcttggacga aggacgtaat gttcgcggtt ttattttcgc attcgctgca
    19201 tgatacgatg tagcagctct taaaggagat gctaaaaatg aagaaacacc ggttagacgc
    19261 acgtatgatt gctattatgg gcttattgat tgcattgatg gtcactttgt cccgactggt
    19321 agcaatcgag accccattta ttaaaatcag tgtcacattt attccacaag tgatcatggg
    19381 cattcttttt ggtccttttt ggtcagggat aggcgcagtt ttagctgatt tagtaggaat
    19441 ggccctcttc tcgaaatctg cattctttat tggatttaca ttgaatgcat ttattgaagg
    19501 agcaatctac ggattctttt tctatcgaaa agagatcact tggaaaaatg ccattctggc
    19561 tacgcttagt gtcacgctga tcatcaactt atttttgaca ccattatggc ttgcgttgat
    19621 gtaccatgtg ccattgttca gctgggtcgt ttgggcgccc cgcctgttaa aaacagtaat
    19681 atggttaccg attcaatcaa ttgccattta ttacgtagga cgtagtattc cttacaaaaa
    19741 aattttaaga agtttagcga tccacgctaa ataaaaaaca agcagtcttc ccatcccaga
    19801 agactgcttg tttttattgt gctgtgaatt gtgcttgata gagccgtcgg taataaccat
    19861 ctttttgaag cagctcatca tgggtgccca tttcgacaat agctccctga tccatcacta
    19921 atatctggtc cgcatttttg attgtggaca ggcgatgggc aatcacaaaa ctggtttttc
    19981 cttccatcat tgctaagaag gcttgttgga tttttttctc tgtcaatgta tcgactgagc
    20041 tggtagcttc atctaagatc aacatagggg gattactgat catcgttcga gcaatcgtca
    20101 acagttggcg ttgtccatct gatatcttta acccttgacc gccaatctta gtatctaatt
    20161 tttccggcaa acgttctaca aattcataca tataagaggc ttttagggca tcattgattt
    20221 ctgcgtcact tgcttccgga ttcccaaaac gaagattttc tcgcaatgta ctgtcaaata
    20281 gccaagtatc ttgtaacacc attccaaaac ttttacgtaa tgtatcacgt tggatagagc
    20341 ggatgtcatg gtcatcgact gtaattgttc cttttgttgt gtcataaaaa cgcatcagca
    20401 aattaacaag tgtggatttt ccggcccctg tttttcctac tatagcaatg gtttgacctg
    20461 gataagcagt aaaattgaaa tcttcgatca aacgctgctt ggggtcataa gaaaaagaaa
    20521 catgatcaaa agagactttt cctttgatat ttttgggttc caaggcatag gcatctggca
    20581 aatcaggctt ttcttctggc tgatcgatca aatcaaatgc tcgttctaga ccagaaaaag
    20641 cggtttgcag ttgtgtcgtg attccagaaa gttcgataaa tggtttagaa aactgacttg
    20701 agtagatcgt aaagctggaa atcacaccaa cagtgattga cgaaccactc ttcaatgcca
    20761 ataggccgcc aactaaacca atcgataaat aagcaaggtg atctacaaaa cgagaagacg
    20821 ggtttgtcaa ggaagaagaa aattgtgccc gttgtccttt ttcatataga tcagcattta
    20881 gcagttcaaa ttgctgttga ctagcttctt cttgctgaaa agctttcacg attttctggt
    20941 taccgattcg ttcggtcaca aagcccgaga tatccccaat aatcctttgc tgtctatcga
    21001 aatttgtttg tgaagcacga gcaacgatcc aactcacaag gaaaataatt ggtgtagaac
    21061 aaagaacgac caatgtcaga agtggactta gacgcatcat aaaaataaac gaaagaatga
    21121 tgattgccac tccagaaaat aattgattga aggccgcaga aattgcaatg gaaatattgt
    21181 ccatatcatt tgtaaatcga ctgacgatat ttccgtggga gttttgatca taataacgaa
    21241 gtggcaatcg attcagatga gcaaatgcat cttttcttaa ttttgcaacg gataagtaag
    21301 ctaaccgatt gcttaacact tggatgagcc attgactgac tacgttgatt aaaacgatac
    21361 caccgaatag aataagtaaa tggaacagtc gattgaagcg gacctgtcct tctccaatca
    21421 tttgatcgat actttgacca atttgtaacg tcatcacaac tgttgcaatc ccattgatga
    21481 ttcctagtat cacagctagc caaatgtctt ttgcataggc agtaaggtaa ggaatgaatc
    21541 ggcggagcgt ctgcaaggat aattttttta ctttcataac tattcttcct cctgtgatgc
    21601 gacgatttcc tgatattctg ctgattgagc aagtaattct tcatgggtac cctgagcagc
    21661 aatcttacct tgatccatca ctaagatatg atgtgcttct tgaatagagc ggacacgttg
    21721 ggagataata atcactgtac tgccaagata agtctgtagc gccttacgca aatccaagtc
    21781 tgtttgataa tccaacgcac ttaaagagtc atctaaaatc agtaatgctg gcttggcaat
    21841 caaagctctt gcaatcgtca gtcgctgttt ttgtccgcca gagaaatttt tcccattttc
    21901 catgactttt gtatctaaac catttggaag ctgacggaca aattcttctg cctgagcgat
    21961 tttcagagct tcccaacatt ctttttctgt ggcgtctttc ttaccccatt gaaggttttc
    22021 acgaatagtc ccggtaaata gtacagaagt ttggggaaca agagcaattt gctgacggag
    22081 atgatccagc tgccaagaac gaacatccat tccgtccatt ataactgtac cggaactgac
    22141 atcatagaaa cgcggaatca attgtgtcaa agtcgttttc ccgcttccgg ttggaccaat
    22201 gattccaagg actgtatttc gcttcagctg aaatgtaata ttttggagag aaagaccaga
    22261 atcaggagta taacggaagt caacatggtc aaaagcgata attgcttcag aagaaaaatc
    22321 gggagtagta ctgacctcag cttcaagaat cgaattttct gtgtctagca cctctttcac
    22381 ccgatttcct gaagcttcgg cacgtgtgaa gatcacgacg aggtttgaaa caacgatcag
    22441 tgccaaaagc atttgattca tataattgat caaagcaaga acttgtcctt gttgcaagct
    22501 accaatattt actttgatcc cgccaactgt cagcaaacag atgatcccga cattcatgat
    22561 caaggttgta gcaggtgata atagggcaga gatattggaa acacgtaaat aattgttcgc
    22621 tagatcattt gtgcttttat cgaacttctc tgtttctttt tttgtctgcg caaatgcgcg
    22681 gatgacacga acaccgctaa gattctgact aacgtgttca ttgagctgat ctagtttttc
    22741 ttgcactttt tggtagagag gaacggtttt tttgatgata aaaaataaaa tgattgagaa
    22801 aataggcaat aatgctaaga aaaacagacc tacttcccag tcgatcacaa aagccatgac
    22861 cactgacccg atacttaaaa acggtgctct gatcaataaa cgaatgacca ttgctaaagc
    22921 tagctgaagc tgattgatgt cgtttgtcat tcgggtgatc aaggtatctg ttccaaaatg
    22981 attcaactcg ttgtgagaga aagtattgat ttttttgatt agctgattac gtaactctgt
    23041 accaaatcct tgtgaagcta ctgaagcata gtattggcaa atgatagcac aaatcagacc
    23101 aatcacagac atagcgacca tccataagcc catttcaatg atttttcctc ggtcattcat
    23161 ttttagtcca ttatcgatca aggaagccat cattaaaggc aggacaagtt cgaagacagc
    23221 ttcaagaaat ttgaagaacg ggcccaaaat gatttgctta cgatagtctt ttgcataacg
    23281 taataactga atcatatgat tcctcctatt atttatttgt ccacaattga aaaaacaagt
    23341 actaccacat tgtagcttag gaaatttaga atgaaaagag aacaagcctt ttcataaatg
    23401 aaagttttat tatgaagaag ctttagaaat tgaatagact atttttttta aaaaaaagaa
    23461 aatgagaaaa catg
//
LOCUS       DAYYKN010000035        23438 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000035 DAYYKN010000000
VERSION     DAYYKN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.035"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..912)
                     /gene="deoD"
                     /locus_tag="ACHBB7_001145"
     CDS             complement(205..912)
                     /gene="deoD"
                     /locus_tag="ACHBB7_001145"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9209107.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(929..1747)
                     /locus_tag="ACHBB7_001146"
     CDS             complement(929..1747)
                     /locus_tag="ACHBB7_001146"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9209108.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1771..2937)
                     /gene="deoB"
                     /locus_tag="ACHBB7_001147"
     CDS             complement(1771..2937)
                     /gene="deoB"
                     /locus_tag="ACHBB7_001147"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM9209109.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(3036..3992)
                     /locus_tag="ACHBB7_001148"
     CDS             complement(3036..3992)
                     /locus_tag="ACHBB7_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9209110.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3992..5125)
                     /locus_tag="ACHBB7_001149"
     CDS             complement(3992..5125)
                     /locus_tag="ACHBB7_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9209111.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5118..6680)
                     /locus_tag="ACHBB7_001150"
     CDS             complement(5118..6680)
                     /locus_tag="ACHBB7_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209112.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6892..7974)
                     /locus_tag="ACHBB7_001151"
     CDS             complement(6892..7974)
                     /locus_tag="ACHBB7_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9209113.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(8040..8438)
                     /locus_tag="ACHBB7_001152"
     CDS             complement(8040..8438)
                     /locus_tag="ACHBB7_001152"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM9209114.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8459..9121)
                     /gene="deoC"
                     /locus_tag="ACHBB7_001153"
     CDS             complement(8459..9121)
                     /gene="deoC"
                     /locus_tag="ACHBB7_001153"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM9209115.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9167..10468)
                     /locus_tag="ACHBB7_001154"
     CDS             complement(9167..10468)
                     /locus_tag="ACHBB7_001154"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM9209116.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10609..11625)
                     /locus_tag="ACHBB7_001155"
     CDS             complement(10609..11625)
                     /locus_tag="ACHBB7_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9209117.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11933..12538)
                     /locus_tag="ACHBB7_001156"
     CDS             complement(11933..12538)
                     /locus_tag="ACHBB7_001156"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9209118.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12734..13657
                     /gene="coaA"
                     /locus_tag="ACHBB7_001157"
     CDS             12734..13657
                     /gene="coaA"
                     /locus_tag="ACHBB7_001157"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM9209119.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13716..15281
                     /gene="guaA"
                     /locus_tag="ACHBB7_001158"
     CDS             13716..15281
                     /gene="guaA"
                     /locus_tag="ACHBB7_001158"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM9209120.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15772..17403)
                     /locus_tag="ACHBB7_001159"
     CDS             complement(15772..17403)
                     /locus_tag="ACHBB7_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM9209121.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17417..19090)
                     /locus_tag="ACHBB7_001160"
     CDS             complement(17417..19090)
                     /locus_tag="ACHBB7_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM9209122.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19103..19420)
                     /locus_tag="ACHBB7_001161"
     CDS             complement(19103..19420)
                     /locus_tag="ACHBB7_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9209123.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19445..20845)
                     /gene="lacG"
                     /locus_tag="ACHBB7_001162"
     CDS             complement(19445..20845)
                     /gene="lacG"
                     /locus_tag="ACHBB7_001162"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM9209124.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21082..21867
                     /locus_tag="ACHBB7_001163"
     CDS             21082..21867
                     /locus_tag="ACHBB7_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9209125.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21870..22562
                     /locus_tag="ACHBB7_001164"
     CDS             21870..22562
                     /locus_tag="ACHBB7_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9209126.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22836..23207)
                     /locus_tag="ACHBB7_001165"
     CDS             complement(22836..23207)
                     /locus_tag="ACHBB7_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM9209127.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         6759 a         5233 c         4224 g         7222 t
ORIGIN      
        1 acaatattca tcaaacatga aaagctgttt tttgaaattg ttccttgtga ctcagagtta
       61 aacggcagat tcacaacctc ttggtaaacg gtgttcaaaa gcttacattt gcaataataa
      121 gtccagccaa gtcaaaaaca tgaggagcta tttttgcttg gatgttctta ttacttaatg
      181 cagggcagct ttttcatttc cttcttattc tgctatcgct gtttccaatc ctacttcgat
      241 catttcattg aacgtagatt gacgttctgc agctgttgtt tcttcaccgg tgatcaaatg
      301 gtcgctaacg gtcatcaaac ttaatgtctg cacatggtat ttcgcgccta agtaataaag
      361 agccgctgct tccatttcca ctcctaaaac gccaagttca gctaattgga acgtctctgt
      421 caaatcatct ttgtagaaag aatcttccga aagaacattt cctacatgaa ccgtatagcc
      481 cttttcttta gctatctcat atgctttcag taatagattg aaatcactga ttggaggaaa
      541 atgaaaggct tggaaatttt tttcgatcat cgaagaactg gtcgctgcac cttgtgctag
      601 tactaaatca cgaacatgga cgtcttttga caatgcaccg catgtaccta ctcgaatcaa
      661 tttctttaca ccatacgact ggatcaattc atgggcatag atggtggctg atggcattcc
      721 cattcctgtt ccttgaacag aaatacgttc tcctttatat ttgccggtaa agcctaacat
      781 accgcgtacc tgattgtaac aaactggatc ttccaaaaaa gtctccgcaa tatatttagc
      841 tcgcaaagga tctcccggaa gtaaaatctt atcggcgatc tcgccttctt tcgcttcaat
      901 atgcacactc attttattgt tcctcctatt acaataatgt caatgtttct ttaaccaatg
      961 ctttaaaaga ttgtttgact ctctgagttg tttctacaac ttcttcatgg ctcagattcg
     1021 cttgcatacc tgctgctaag ttggtaatac aagagattcc taacactttt aatccgctgt
     1081 gagctgctac gatgacttct ggaacagtcg acatccctac tgcatcggct cctattgtcc
     1141 gagacattcg gatctccgca ggcgtttcat aagttggtcc agaatagccc atatatacgc
     1201 cttctttaag atccaaacct agtttttctg ctgctgcttt tgctgttgct tgatagtcag
     1261 gcgtataagc ttgactcata tctggaaatc tagggcccat tgcatcttca ttcggaccga
     1321 tcaaaggatt ttcaccagta aaattgatat gatcggtaat cagcatcaaa tctcctggta
     1381 caaatgattc gttgacacca ccacatgcat tagtcacaat gactgaatgg gcccctagtc
     1441 cggccatcac acgtactgga taggtcactg tttgcatgga atgaccttca tagtagtgga
     1501 atctcccttg catggctaac actttcttgc ctgccagaga cccataaact agctttcctg
     1561 catgcccaac tactgtcgaa acaggaaaga acgggatctc atcataagaa atcattacag
     1621 catcttcgat ttcatctgct aattcaccta atcctgaacc tagaatcaaa ccaaaatcaa
     1681 tttcttttac tcctttatca aggataaact gggtcgtttc ttgtaacatc tcgcttaact
     1741 tggtcatttg ctttctccta tttaaaaaga ttattttaat aatttcaaga agctttcgcc
     1801 attttcagtc gaagaaacac taaagttctc agcaatcgtt gctgaaatat ctgaataata
     1861 tccttgaggc aatgcccctt gttctttcat ttttttactg taaaccaata acggtacata
     1921 ttctcgcgta tgatcggtac cagtaaacgt cggatcgttt ccgtgatcgg ctgtaatcat
     1981 tagcaagtcg tcttcttcca tcgcttcaag caattctgga attcgtaagt caaattcttc
     2041 aatcgcatgc gcatagccga ctacatcccg acgatgaccg tataatgcat caaaatctac
     2101 taaattggta aagctgattc ctgaaaaatc gtttttcata actttcagca gctggtcaac
     2161 gccatccata ttgcttttcg tgcggacaga ttcagtgatt ccttgtccgt tgaaaatatc
     2221 gttgatcttt ccaacagcaa tcacgtcctt tcctgcttct ttcaaagaat caaggactgt
     2281 gtgtccgaat ggattcaacg cataatcatg gcgattgctt gttcgtgtga agtctcctgg
     2341 ttcacccacg tacggacgtg caatgattcg gccaatcata tacggatcat cttttgttat
     2401 atcacgaacg aattggcaaa tccgatataa ttcttctaaa ggaatgattt cttcatgtgc
     2461 cgcgatctga aggacaggat cggcagaagt atatacaatt aaatcgcctg ttttcatttg
     2521 gtgttcaccg taatcgtcaa taacagctgt tccgctataa ggtttgttac atacgacttt
     2581 acggccagaa aattcttcga tttgtttgat cagatcatca gggaatccat taggaaacac
     2641 acgaaatgga gttttgatat tcaatcccat gatttcccag tggcctgtca tcgtatcttt
     2701 ccctacagaa acttcttcta atttcgtcgc atagccttga tgatctttca ctgattccac
     2761 accttcaaga ggacggatcg ttcctaaacc aagctgctct aaatgtggaa cgttcaatcc
     2821 tgcttctttc gcaatgtgtc ctaatgtatc agaaccaaca tcaccgaact tatcggcatc
     2881 tggtgcttct ccaattccta ctgaatccat gacgatcaga tgtacgcgtt taaacatgat
     2941 tattccttct ttctttttat ttttattgcc tttaaaacaa aaagggcgag acgaaaaaca
     3001 cttcccgtaa cccacccttt ttattcgcta aaagattatt ttgatttgat atagttcttc
     3061 ccgtttgctt ttggaccaga agctttccct aagaaaacaa ccagcacaat gattgtcagc
     3121 aaatatggag caatttgcag caatactggc ggaatcgaag aaataactgg taattgtgcg
     3181 ccaatgatac tcaagctttg agcaaaacca aagaacaatg aggcacccat tgcccctaaa
     3241 ggattccact taccaaagat catagcagcc atggagatga acccttgtcc aacgatcgta
     3301 ccagctgaaa agttaccgga aatcgactga gcaaaaacag caccgcccat accgcctaac
     3361 aagccagaaa tcagtacgcc tgaataacgc atagcatata cattgatacc taatgtgtcg
     3421 gcagcttgag gattttcacc aactgaacgg aggcgcaagc cgaatttcgt cttgaagatg
     3481 atcacccaag ctaggacagc aatcaaaata gcacaataag caggtaagaa tgtattttta
     3541 aagaaaattg gtccaatgac cggaatatct gctaatcctg ggaaagaaaa ataccccaga
     3601 ttctgcgtga tgttatcagt ttgaccttta ccataccatg ctttgatcaa gaaaacacct
     3661 aaagcaggtg ccatcagatt aattactgtc ccgctgataa tatggtcagc acgcaaactg
     3721 actgttgcta ctgcgtgaat cagagaaaat aggataccta cgacgccacc cactaaaata
     3781 gcgatccatg gtgtcataga accaagtgat ggactcatcg tcaagttgaa aacgatggaa
     3841 ctaaaagcac ccatgaccat gattccctct agccctacgt ttacgatccc gcttcgttca
     3901 gaaaaagttc cgcctagtga cgttagaatt agcggtgttg aataaaccag tgtagaagta
     3961 aggatcaagg caattaattc gatagtcgac attatagttc gcccccttca tggttttgat
     4021 tcgttggttt cgattctatt gtcgcaacga tctcttcttg ttttttccct tggaagactt
     4081 tagccatgac gaaacgaatc aggaagttga tcccgacaaa gaagataatc aaagcaatcg
     4141 atacattgac gatctcaaac ggaacacctg ctcctgtctg catgccaagt ccaccgatct
     4201 tcaaaatact gaacaagatc gctgataaaa gaataccgat tgctgatcca gaaccaagca
     4261 aggaaacagc cattccgtca aatccaatag atagcgaagt cgtttgaaca aagaagtttt
     4321 gatacgttcc taatccttca accacaccgc caagccctgc caaagcgcca gaaatgacca
     4381 tcgacaaaac gatcgtacgt ttactactca ttcctgcata ctcagaagca aaaggattca
     4441 atccaacaga acggatttca aatcctaaag tcgttttctt catcagcagc caaacgaaaa
     4501 taagcgcgat gattgctaaa aataatcctg agtttacgcg agagccgcca aaagcttgtg
     4561 ttaaccatgg taaccgcagt gaggcatttt gactgatcat attggaagag tccaaacttg
     4621 agcgatagct ttcaggcatc acttcatgta aagcaaatgt gcttacgtat aataagatat
     4681 agttcatcat gattgttacg atcacttcac ttgttccaaa aaatgctcga agtaaacctg
     4741 gaacagccgc tgccatcgca ccagctaagg cacctaagat cacgcataac ggaagcaaaa
     4801 caacttttgg cagatctgga aacgcaagtg ccacccagac actgacgatc cacccgcaaa
     4861 gtgcttgtcc agaaagccca atattgaaaa acccagctga gttagctaca gaaaaaccta
     4921 atgctgtaaa aatcaacggc gttgcttgcc ttaacgtttc acctatccct ctttgcgaac
     4981 caagggctgc atctagcata gaagaataac cttggatggg ctgaaaacca aaagcagcca
     5041 tcaatatcgc gcctaagatc aatcctaaaa caactgaaag tacaggcact aataaattac
     5101 ggaatttttc gctgcgatca ttcatgtgcg ccagccccct tttctaactc ttttcgtgct
     5161 tcttctaagg tatagcccgc catcaataga cctaattcat tttcagatgt ttctttcgga
     5221 tcaacgatac cgacaatttc tccttcatgg ataacagcaa tacgatccga aacatttaaa
     5281 atttcttcta attcgaaact gatcagtaac actgctttgt atttgtccct ttgttcgatc
     5341 agacgtttat gaataaattc gatcgcacct acatccagtc cacgagttgg atttgcaaca
     5401 atcaacagat caggatctcg gtcgacttca cgagcgataa ttgctttttg ctgatttccg
     5461 cctgataaag cttttgcggg aacaagttca ttcgttgttc gaacatcata ttcttcgatc
     5521 aattctctgg catgttcatt cattactgaa taattcagca tgccgttttt actgtaaggt
     5581 ttttgatgat aggtttgtag tgcgatattt tcagataatg tcatatctag gactaacccg
     5641 tatttatgac ggtcttctgg cacgtgtcct acaccatgtt cagtgatttt tcggggtttt
     5701 ttattagtga tgtcttcccc ttttagcttg atatgtccgc tttctgcctt tcgcaaacca
     5761 gtcaaagctt ggatcaactc cgactgcccg tttccatcga ttccagcgat accaagtact
     5821 tcgccagcac gaacctctaa gttcaagttt ttcacggctt ctaatccacg attttctttc
     5881 accactagat tttcaataga caaaacgact tcttgaggct tcgcttcttt tttcatcgtt
     5941 ttgaatgaaa ccgttcttcc aaccatcata tcggctaatt gttgtgaggt tacgtcttta
     6001 acgttgactg taccgattcc ttttccacgg cggataactg tacagcggtc agctactgct
     6061 ttgatttcat ctaacttatg cgtaatcaaa atgatcgact tgccttcttt gactaattcc
     6121 ttcatgatca cgattaattc atcaatttct tgaggggtca atacagctgt cggctcatca
     6181 aaaatcagta catctgctcc tcggtaaagt gtttttaaaa tttctacccg ttgttccatt
     6241 ccaactgaaa tatcacgaac gtaagcatct gggttgactg acaatccata ctgttcagaa
     6301 actttttgga tctcttttcg tgctttttta tggtcaagca tccctgcgcg actaggctcg
     6361 cttcccaaca cgatgttttc tgttacagta aaggcatcaa caagcataaa gtgctgatgg
     6421 accatcccaa tccctaattg gttggctttg gtcggacttg tgatctttac ttctttccca
     6481 cgcatcaaaa ttttccctga tgtcggctct aataatccgg aaagcacgtt catcaatgta
     6541 gatttgccag caccgttttc tccaagcaac gcatgaattt ctcctgcctt tacttgtaaa
     6601 ttgatgttgt catttgcctt gaatgttcca aattctttgg taatattctg catttctatg
     6661 acaatatttt cttgaaccac ctttttttca catcctaacg atttctatct agggcaacga
     6721 ttgactaaga ttcacgctta gtgagcgttc ttcttttgaa acaaacatta cttcagggag
     6781 ttgttccctt atccaaactg tcacaaggca gcttggataa atgaacaatt cttgttctaa
     6841 tcaaaccata gccttgtcaa acatgtgtct aacaaggcta tagaaatggt tttagttttc
     6901 ttcaggagtt tctggaactt ttacgtcacc tgaaatgatt ttttcttttg cttcatcaac
     6961 tgcttttttc gcttcgtcag aaagttgtcc ttctgttaga ccaacaccgt cttctttcaa
     7021 tccataaaca gtgtgttcgc cgccagggaa tttaccgtcc gctgattttt gtgccaaatc
     7081 ttctactact gttccaactg ctttcagtgt agatgtcaat gtgaagtttt tcttttcgcc
     7141 gtttaatgtg tattcgcctt cgtctgattg gtcgcggtca acaccgataa cccaaacttt
     7201 tttgtcgcct gattcgttca atgattttgc ttcttggaat acaccgttac ctgtaccgcc
     7261 tgaagcatgg aagatgatat ctgcgttttg tgcatacata ccttgagcaa ttgaacgtcc
     7321 tttatctgga gcagagaagt caccagcata ttgattcaat actttgattt cttttcctaa
     7381 ttctttcgca cctgcatcaa caccggcttt gaatccagca tcaaaacgat cgattacttc
     7441 accttttaca ccgccgataa agccgactac gtttgtttca gtcgtataag ctgctgcaac
     7501 acctgcaaga taagaagctt cgttgtcttt gaaagttgca gatacaacgt tatctaatcc
     7561 atcgatgaca tcatcgataa taacaaagtt cgtacctgta ttgtttgttg cttgttcttg
     7621 gatcgctggt tttagtttat aaccaatacc aaaaatcgtt ttgaatccag cattcaatgc
     7681 ttggtcaata tttgggatgt aatctgattc atttgaagat tggaaatatt ggaagccatc
     7741 atttcctcgt gaaagtcctt gatctttccc ccatttttct agtccttccc aagctgactg
     7801 gttgaatgaa cggtcgtcta caccacctgt gtctgtgatc aatgctgcag ttgttgtgtc
     7861 tgatgagctt ttgccaccgg atgaatcagt agagccgttg ttgccgcatg cacctaaaaa
     7921 taaccctgct gctagtgcga cagcacctaa accaaataat tttgcttttt tcatttctgt
     7981 aaagcctcct aaaataattg ttttattttt caacagtcag ctgactgaat gaacatgagt
     8041 tatagatctt tttcagtaaa tgcataaggc agcagtgcac gaacggtcgt tgccttcgtc
     8101 acaccattgt ctcctactag cgtaaccggc atatcagggg aacagaattc tgccattact
     8161 tgtcgacatg caccacaagg agagatagga tcgggggtgt gccccgctac gactagatgc
     8221 gtaaatgttt tttctccttc agatactgct ttgaaaaaag ctgtgcgctc ggcacaattc
     8281 gttaatccaa atgatgcatt ctcaatgttg atgccttgat aaatcttgcc tgattccgtt
     8341 acgagacaag cgcctaccgg aaaatgggaa taaggaacat aggctttatc taacgcttca
     8401 atagctgtgt tgatccattc ttgtttgagt tctgccatga gcttctcctt tgaaaaaatt
     8461 aatagccttc accagttgaa ccagatacga ttgctactcc tgcgcttgtt ccgattcttg
     8521 tagcaccagc ttcgatcatt gccattgctt cttcttcgtt atggataccg ccggaagctt
     8581 tgacacccat ttctggtcct acagtttcac gcatcaaacg gacatcctct acctttgctc
     8641 cgcctgttga gaaaccagtt gatgttttga caaaatcagc gcctgcagct tttgctattt
     8701 cgcaagctcg gatgatttct tctctatcta ataacgatgt ttcaataata actttgacaa
     8761 gcgcacgatc ttttgctgct tcaacaactg cttcgatatc tttttggact tgttgatcat
     8821 cctttgattt caaagcaccg atattgatga ccatatcgac ttcatctgca ccgttattga
     8881 tggcatctgt tgtttcaaat gcttttgtct cgggtgtgtt tgctcctaga ggaaacccga
     8941 ttactgtaca tacggctact ggctcacctt gtaattgttc tttcgctagc gcaacccatg
     9001 ctggattgat gcagacagaa taaaaatggt atttttttgc ttcttcgata atttgcatca
     9061 cagcttcttt agatgcgtct gctttcaaaa tagtatggtc gatcattcgg tttagttcca
     9121 tttcttttcc ccttttcttt aaaacggtaa agtccgcttt tttcctttat tctgtgatga
     9181 tgtcatgaat caacgttggc ttcattgccg attcaccaat ttcaatattt ttatataata
     9241 attcaatgac agaagtgatt tcttcgtcgt tactatagat cgtcaatagt gactcacctt
     9301 ttgtcacagt gtcaccaatt tttttatgca gtttcaatcc aacagcgtga tcaatatctt
     9361 cttctttggt tttacgccca gcacctagca tcattgcagc aattcctaat tcatttgcca
     9421 cgatttttga cacaactccg ctttgtttag caggaagctc cattgtgtag cgtgcagtca
     9481 aaattcgttc tggatgttcc acgatcgctg ggtctccccc ttgattttgg atcatttctt
     9541 tgaattttgc taacgcttta cctgattcca gagcttcttg aagcaacgtt cttgcttcat
     9601 cggttgtctt agctttgcca gcaagaacaa ccatttggct tcctaaggcg tagcacattt
     9661 ctactagatc ttctggacca tttccttgta atgtttcgat tgcctcgaca acttctagac
     9721 tatttccgat agcttcgcca agaggttctg acatatctga gataaccgcc actgtttctc
     9781 ggttagccag tttcccaatg ctcgtcatgg tctttgccag acgtcttgca tcttcgatgt
     9841 tcttcatgaa agcaccgtct ccagtggtca catccaaaac gattgcatct gctcctgccg
     9901 cgattttttt actcataatc gaactggcaa tcagcggaag agaatcaacg gtcgctgtga
     9961 cgtcacgcaa ggcatacaat tttttgtccg ccggtgccaa gtttcctgat tgaccaatta
    10021 ccgctacttt actttcatta acaatgcgta caaattcttc atccgaaagc tcgatctgaa
    10081 atccgggaat tgcttctagc ttgtctagtg taccacctgt gtaacctaga ccgcgtccag
    10141 acattttagc cactgtcact ccgacactag ctactagggg ggctaaaatc aatgtggtcg
    10201 tgtcaccaac gccacctgtc gaatgcttat ctacctttat tccttcaaga gaactcaaat
    10261 cgatgacatc accagaatta gcaatcgcca atgttaagtt cgtgatttct tcatttgtca
    10321 tgtcttgata aaaaattgcc atcaataatg cactgacttg ataatctgga atctttccat
    10381 tcgtatagtt cgtgacgatg tactcgattt cctctttcga cagctcgttt ccatccctct
    10441 ttttttcaat cagatccacc attcgcattg cctgatcctc cttgtcttca ttcggtatta
    10501 tacattattt ttaggaaagg taaaccactt tagtaaactg gtttactaaa aataaaaaaa
    10561 gcactaacta gcctttgtaa atgcttacat aagcaatata ccgccgagtt agccctttgt
    10621 caatactttt acgctctcac gaataattaa ttttgtttcg aacactttgt tcggaacttt
    10681 tctttgagga aattcgatcg tatcgatcag gaaacgtgca gcataaaatc caatatcaaa
    10741 acttggctgc tgcactgtcg tcaattgcgg aatcatgtat tctgacagct ttaatccatc
    10801 gaatcctatg actgagatat cttctgggat ttttttgccc aattcatcga tcgcctgata
    10861 gcatccgatc gccatggcat cattcccgca aaaaatagcc gtgatatctt tatgtttgag
    10921 taattttttt gatgcagtat atccaccttg gacagtcaac tctccatgtt caacaaattt
    10981 cccattaaaa ggcaggccat gatctttcag cgcatgacga tatccgttga aacgttcttc
    11041 taattgatag tatccagtgg tttccttcag catgccgatc ttcgtatggc cattttcaat
    11101 aaacagcgaa acagcttgat aggcgccctc atattccttt acgataagcc gtccgttatc
    11161 ccgacgattc aatccgcgat cgattaaaat gattggcatt ttttgataaa aaccgctgtc
    11221 taatgcatat ttctcaggta aaatatttgg tgtggccagc aagattccgt ccactgagcg
    11281 gtgggaaagc tcttgtaaat acttgatctc attttcttgg ctatgacgag aattgcataa
    11341 catgatcaca tagcccaaag gattcaaata gctctctaca ccttcgatca gtttggagaa
    11401 gaagaaatca gtgacatcag ggacgatcat tccgatggtc tttgaatgtc gattgatcac
    11461 atttgaagcg aaataatcgg gcttataatg atgttcattc acgatcgaca acactttttt
    11521 tcttgtctta tcactaaatc tgcttccttt gttattcaaa atctgtgaga cagttgttac
    11581 tgatacgcct gccatttctg cgatatctct gatggtcagc ttcatcctat tggctccctt
    11641 ctatctaaat tccctatcag tgtaccaaaa cccccaagaa aagaatagag aaaaaaggaa
    11701 agtatgtttt ttgataaaaa cctcctttat ctaccgactt ggcagtcttc tatacaagtt
    11761 ctgtatctta tttattttat cgtaaaaaac aacgactctg ttcctactat ttctttttga
    11821 agaaaaaata gttctttgtt tttaaaaact tctcaacaaa aaaagaactg atacgtaccc
    11881 gtacatgcac gcccagttcc actctttcgt cgctccacta tttttcagct agttattctt
    11941 tcacactttt gatgatataa tatcctttat cttttgtgac gatttctgca ttaccaaatg
    12001 tttcttccat tttcttttgc gcactcggcg ctccttgttt cttctggatc acaattgtca
    12061 gtgtaccgcc cttcttcaac cgaggatatg cgcctgtcaa aatttcatgt acaacttttt
    12121 ttccagcacg gatcggcgga ttgcttacga tagctgcata ggtttcttca tgaactgctt
    12181 catagatatt cgattgatgg atatcaacat gttcgatttg gttacgcttt gcgtttcctt
    12241 gcgctagact taccgctcgt tcattaatgt cgaccatttc aacaaaccgc tgtgtcgcaa
    12301 atgccaaagc taacccaatc ggtccatacc cgcatcccac atctaagatc tttccttcat
    12361 caggtaactc ttcccaatcg aaagcatcaa tcaggacacg tgaaccataa tccactgttt
    12421 cacgtgaaaa tacaccagaa tctgttacaa attgaaaggt tctgcctttt aactcaaaag
    12481 cccattgttc aaaatcgtgc gccgtatctg ggcgttcaga atagtaatga tttgtcatgt
    12541 cttccatcct tctcatttct ttcatgacaa cagtctatcc ttttatgtat ccgtgcgcaa
    12601 gtcaagaata agactccgtt tctttctatt ataaagatta tacaaaaaga tcggcaatct
    12661 ctcttaagtg gtggggtttg cgtttatttt gtgttaaaat agttccattg atgggagaac
    12721 gcaggaggct aatatgaatg atccaatgaa ctactaccgt gtggcaagag aagaatggcg
    12781 agaattttat cgtaatgggc aagcaccttt gacacaagat gaactagata atatcaaaag
    12841 tttgaatgac cgcatttcca tgcaggatgt tcgagatatc tatgtacctc ttgctcatct
    12901 gatttatctt tatatgaaag aatttgaatc tttgttcttg agtaaaggtt tatttcttca
    12961 taaatacgta gaagtaccgc cttttattat cgggatcgcc ggaagtgtag ccgttggaaa
    13021 aagtacaaca gccagactgc tgcagacgat tttgtctcgt accttcaaac gaagaaacgt
    13081 tcagctgatc acgactgacg gatttcttta tccaaatcgt atattaaaag agcgtggaat
    13141 catggaccgt aaaggatttc ctgaaagtta tgatatggag atgctgatcg atttcttgaa
    13201 ccgtgtaaaa tctggtcagg aagagataaa agtacctgtt tactcacatg atgtgtatga
    13261 tatcattcca gacaagtacg aattgatcca gcagcctgat atcttgatcg ttgaggggat
    13321 caatacgctt caattacctg cgaatcagca aatctacgtc agcgactttt ttgatttttc
    13381 tgtctatgtc gatgccgatc ccgtgctgat cgaaaaatgg tatttggatc gttttggtgc
    13441 gttactggat acggctttcc aaaatccaga gaattattat tatcgttttg ctatcggaga
    13501 tcgtgaagaa gcctttaaaa tggcaaaaga tgtatggaaa aatgtgaatt tgaaaaattt
    13561 acaggaatat atcttgccta cacgaggaag agccgatata attctccata aaactgaaaa
    13621 tcatatcatc gattcgattt tcatgcgaaa atattagcat tttaaaatag tttgactatt
    13681 ttgagataaa atttaaaggg ataggggtaa taaatttgac aaacgttgcc gatatgacga
    13741 acgttgaaaa gattatcgta ttagactacg gtagtcagta taaccaattg atcacaagac
    13801 gtattcgtga tcttggagtc ttttccgaat tattgagcca ccgcattact gcagaagaag
    13861 taaaagcgat gaatccaaag ggaatcattc tttctggcgg accaaacagt gtatatgatg
    13921 agaatgcatt tgggatcgat cctgagatct tcaatttagg tattcctgta ttagggattt
    13981 gctatggtat gcaattgatc acgtacaaac ttggcggttc cgtagagcct gctaaaaacc
    14041 gcgaatatgg gaaagccttg ttagaagtaa caagcgatca agctgtcttg tttaaagaaa
    14101 caccaaaaca acaaacagta tggatgagcc atggtgactt ggtaactaaa gttcctgaag
    14161 gctttgaaac agtcggaaca agcaaagatt gtccgattgc ttctatccaa gacacaacac
    14221 gtaattttta tggtatccaa ttccatgcag aagtacgtca ttccgaatac ggaatcgaat
    14281 tgctgcgcca ctttgccttt gatgtttgtc actgtgaagg taactggaca atggataact
    14341 ttatcgatat gcaagtagaa aaaatccgtg aacaagttgg tgacaaaaaa gtcttgcttg
    14401 gtctttcagg tggcgttgat tctagtgtcg ttggggttct tttacaaaaa gcgatcggtg
    14461 accaattgac atctatcttt gttgaccacg gtctgctacg caaaggcgaa gcggaacaag
    14521 taatggaaag tctaggcgga aaattcggct tgaacatcat caaagtcaat gcaagagatc
    14581 gcttcttaga caagttgaaa ggtgtatctg acccagaaca aaaacgtaaa atcattggga
    14641 atgaattcgt atatgttttt gatgacgaag caacaaaact tgctggtgaa gaaggcgtct
    14701 ctttcctagc acaaggaacg ttgtatactg acgttatcga atctggtaca gaaacagcac
    14761 agacgatcaa atctcaccat aatgtaggtg gactgccaga agatatgcaa tttgaattga
    14821 tcgaaccatt aaacacatta ttcaaagacg aagtccgtga attagggact cagttaggca
    14881 tgcctgattc aatcgtttgg cgtcagccat tccctggtcc tggtcttggt attcgtgttc
    14941 ttggcgaaat cactgaagac aagctagaaa tcgtacgcga atctgatgct atcttgcgtg
    15001 aagaaatcgc agctgctgga cttgaccgtg atatctggca atatttcact gttcttccag
    15061 ggatccgctc tgtcggtgtt atgggtgacg gacgtactta cgactacaca gtcggtatcc
    15121 gtgctgtaac ttcaatcgac ggaatgaccg ctgactttgc ccgtatccca tgggaagtct
    15181 tgcaaaaaat ctctgtacgt atcgtaaacg aagtagccca cgtcaaccgt atcgtgtatg
    15241 acatcacttc taaaccacca gcaacagtgg agtgggaata aagagccaac ataatatgga
    15301 gggggaatcc ctataaatca acattcttca atagctaaaa tgcttgattt tcattaaaaa
    15361 taagatggtt ccctaccaaa agagactata atataagtta atttgtttca ttcttatcat
    15421 aaaaagaagt tctcgaaaaa cgaactgttt tccgagaact tttttctatc catctaagtg
    15481 ctcaaatcat attcacgacg gaattctaag gttacccttt cttaaacaaa ctttatgtta
    15541 tctgcacaac tttttgcaat tttttcattg cttcttacga aatcgttgaa ctctctttag
    15601 cataatacaa agtgtagatg aagtctggtg ctttactcgt tttggtaaaa atgactggaa
    15661 atcgtttctg gttatttttg gcaaaggcga ggtgcaggtt cgattcctgc cttctacgct
    15721 aacgatgtgg ctgaagggaa cctccgatct tgaatcgaaa cgatccgcag tctattgttt
    15781 caccaatgaa acaaaatcaa cgttcgtcca agcatttcct gaagccttgc tataaacccc
    15841 aatttcacaa acacctgttg taattggaat gtctttgatt gtaatttttg tccattcact
    15901 tgttttaaca accgcagtat ttagttccgc tccaccataa tttttgatat ataactgatt
    15961 agtatcaaac tctcccccag acattacata ggcactcaat gtgtaagttc ctttgcttat
    16021 gccactaaca ttttgataag ctgatgcctc gtatgcttga tcctgccaaa aagttagttt
    16081 gtagtcccca aaaaaagtgc ccgcttccgt tttgatcgta cccttagcgg ttccttgttg
    16141 aaaccaggtt tgccaattgg aaggactggt cgttggtcca tcgctttcga aacttgcatt
    16201 tttaactaga tttatttgcg cccctgcttg tttaaacgca cgcaagcttg gcaaggcgtt
    16261 tccagcaaaa tcaaacatcg cttggttgtc ccattcgttt cccatagtgg attgaacact
    16321 gagataattc atacctgctt gtgttgccca actcacattt ccgttgtacc aaagaggttc
    16381 ccaatagaag aagcccgccc cgcgattatt cggtactgcc tgaatccgat cggcaagatc
    16441 cagcaaatac tctgcttgtc ctgtcggtgt tggttcatat ccccccgcag ctgcttcggc
    16501 ttcaccaaaa gcatttggca tagtatcggc attcgctgtt gtatgcccat aagctgtttc
    16561 aactacgatc acttctttac cataccgttg gctgatcgca ttcatattgg tttgaagttc
    16621 attcattgaa ccgtgccaat acggataata cgacactcca attaaatcaa atgacacacc
    16681 tcgactcgtg atttcatcaa accaccaagt aaatgctcct gtatcgccac catcagctaa
    16741 gtggagcatt actggagttg ttttaggttg agtgtctcga acagcttgga taccagactt
    16801 caaaaaagca gccaatcgat caaattctcc cccgccttct ccccaagact tgccataagg
    16861 ccagagcatt ccgctattca gttcattgcc gatctgaacc atatcaggat aaacgtccag
    16921 ctgcttcatt tccgacagaa cgttctttgt atagtcgtac aatgccgtat tcatttcttc
    16981 aaaggtcaga ccttgccatg ctttaggaag attctgtttc ccaggatcaa cccaaaaatc
    17041 actgtagtga aaatccagca gcaccttaag tcccagattt tttgctcgtt ttgcaaggtc
    17101 aatcgtcgtt tccagatcat ttgtccctgc tccataactt tctccattac tattataagg
    17161 atcctgccac aaacgcagtc gaacatagtt gacaccatta ttttttaaaa tctctaaggc
    17221 atcttttgaa atatcgtctt cataatattt tgcaccagat ttttccatat ctgacaggat
    17281 cgaaatatct gctccgcgaa taaattcgct ggcagatgct tcaacagttt ttgaaacagc
    17341 ccccaaagcc cctaacacag ctgcaaataa aacagcttta cgcaaccaaa ttcccatttt
    17401 cattatggct cctcctttat tgaaattggt tcagaataaa ctgaacagct ttttcaggat
    17461 ctctcgttaa tccgatatat tctgctcctt ttgtcgcaac cagcttgatt cctaagcgat
    17521 cggtgtcttc tttgatatca tcatagtaag aattgacttg cggtgctaag accaccatat
    17581 caaaatcctg cagcatttcg taatgagaac cataagcacc cgccattgca ctgatcggtg
    17641 ctttcgattc agatgcacct ttttttaaag cattagctaa catcgcactt gtccctgcgc
    17701 cagcacaaag taccaacaca ttgatttctt tatttgcatc gctgataact tgactcgctg
    17761 ccacaggtac ttcctcacgt acatcggcag aagcaccttc ctcggttgcg atcacctctg
    17821 ctttttgtgc ttctttctct actaagacag catcgtatgc tttgcaaaat ggtagataga
    17881 tcaggaagtc aacgacgatt agaaccgctg ctagaacaaa agctaagata cccatgcccg
    17941 ttcctagaac caaaccgatc ggtgctggag ttgcccaagg caaaacgtac ataaagctgt
    18001 tcatgcccaa tacatcgaca aaaaacttga acaggcaaac gtttacaaca ggtgttaaca
    18061 aaaatggaat aaaaaaataa gggttcaaaa tcatgggtgc cgcaaatagc aatggttcgt
    18121 tgacagcgaa acttactgga ataaaggaag ctttcccaac tgccttcaac tgcttagact
    18181 tagcaaagag taggaagata aaaggaacaa ccaatgttgc cccagttcct cccatagttc
    18241 ctacaaaatt ccctaaaccc accgtcagta ctttagaagc ttgttcaccg gaattgaaca
    18301 attgcagatt ggtttccaca ttggcataga taattgccgc aattgccggc tccacgatcg
    18361 acggtccatg aacaccgaca aaccaaaaca gcgccatcgc tccccagata atagcgattc
    18421 ctaagtaccc gtcagctgct gtaaatagcg gctgcagcac cgtaatgatt gcttctgcaa
    18481 atgagatttg gaagatttgt cgaatgccta aatcaattaa aacggccgca aatactgaaa
    18541 aagagaaagg aaaaatatct cggaaggttt gcgaaatcgt tccaggaact tctttaggca
    18601 ttttaatggt aatatctttc agcacacaaa acttatataa attaactgtt aaaaatgcag
    18661 cgacaaagga agccagtagt ccctttgtcc ccatatattc cgttgaaaat ccaccttcaa
    18721 tcggagagac gcttaacaac aggaaactta ccacagaggc cagcatcaca gaggtggtgt
    18781 tgattacttt gtttttcggt aagcgacgat tcatcgaatc agccaagcag cgagcagtgg
    18841 ttgctgaaac gagtaaccct acaacaccca tcgaataatt atagactttc catagccatg
    18901 tggagacaga ttcaggccat tggtaaccaa aaatctcagg aaccgcagca atcaaaatga
    18961 aaatgcttga aaataaaata gctggcatcg ctgttaaaaa tccgtcacga acggctccta
    19021 aataactatt acgtgagatt ttttcaaaaa atggtttccc tttttcgatt tgtttaatga
    19081 ttccatccat agcgttcttc tcctatcctt gattctgtct gtacagtaca ataaagtctt
    19141 gaatcagatc tctcagcaat aaagtggtca ttaaatgatc ctgcccatgg ataaaaatga
    19201 atcccatctc catctctttc ccagatgctt cttccgctaa aatttttgtt tgcgaattat
    19261 gtgcaagggt taaattttcg tcggcttctt tcaacgccga atcaacatgt tcaaagtttc
    19321 cttgtcttac ttctttcaat aatttcaata atgtactgcg agcgtcgcct gaatatgcaa
    19381 cgatctcaaa accaagcatt tgtaattcct ctttattcat cttttcttcc tccattatca
    19441 cttattaaat aatcgttttg gtttcactga ccaacttgta ccagtaagct gattcttttg
    19501 ggtaacgctt ctgggtttca aaatcaacat aaaacaagcc ataccttttg ttatacccat
    19561 ttgtccatga gaaaagatcc ataagcgacc acaaaaaata ccccttcaca tttaccccgg
    19621 ctgttatcgc cttgcttaag gattccaaat agactctcaa atagtcgatt ctcggctgat
    19681 ccataataat gccatcttca aactgatctt tataccccat cccgttctca gtgatataga
    19741 ttttgttgta atgtgggtaa tcgcttttaa ttcgcagcaa caagtcatat agaccttctg
    19801 gataaatcag ccagtcccaa tcagttcgag gaattccttc tttataaata cgttctccga
    19861 ttcccttcac tttataaaca gaggtccctt tttcacccgt accgttatga tggattgcat
    19921 tttctccatc gtaagctttg acaaaatggc attggtagtg attgatccct aagtagtcat
    19981 tacgcgtgga tgcttttttc aattcaacga aatcttcttc aggaaaatga tagctggcct
    20041 gatttgcttc acatatctca tcaagagcag tcaaggtttc agttgaataa tagcctaaat
    20101 aggtagcatc taataagaaa cggatcgaca atgcatcgtc taaaaaggca gcatgcttat
    20161 cttccggcgc gtctgttgcc gcatatttcg tctctaatga gtgaactaca ccaatttcac
    20221 ccggaagttc gttttctttg aaatagttca ctacacgggc atgagcaacc atcatattat
    20281 gaagacaggc gacgatcttt gtaaaatcat atttgattcc tggtgggaaa acacctaaca
    20341 aatattgatt ggttgcaact ggatagattt cattaaaggt actccacacc ttgacttctt
    20401 tgaattcatg aaaacaaaaa atggcatagg aaacaaatgc ttctattgtt tctcgattca
    20461 aaaaatctcc atggtcaaac aaacgtttag gggtatcaaa atgatgcaac gtcacaaacg
    20521 gcgttacgtt tcttttctta cattcttcga acacacgatg ataaaaagcg attccttctg
    20581 gatttggttt cccagctcca tcaggaaaaa tgcggctcca agcaatggat aatcttagac
    20641 catttacacc aaaacgttca cacaattcaa tgtcctttgc atattgatga taaaaatcac
    20701 ttgccggatc gggactgaat cgcccttgtt cctctaaaaa gtcatcccaa gcaacagcgc
    20761 ctttgccacc ttccttagtg gctccttcaa cctgataggc tgctgtggct ccgccaaaaa
    20821 taaaatcttc tggtaattta agcatgtaca tctctcctca aatctataat cagttttcgc
    20881 tctttctaac tgattgcatt gtaaacgcat ccaaaacatt tgtcaacaaa aacaaacaca
    20941 aataatcata aaaaccagaa aacagcaata aaatctattg taacaaacat aaaaaccacc
    21001 ctaaatgttt acttttgttc gaattagatt gactgttgtc cgtttatatg atagattatc
    21061 cttaaattag aggtgaagac aatgttgaaa aaagaaagat tgcttactat cgtagaaatg
    21121 gtcaataaaa aaggaatcct taccgttaat gaaatcatca atcaattaga tgtttctgat
    21181 atgaccgttc gcagagattt agatgaatta gaaaaatcgg gcaagctgct gcgtgtccat
    21241 ggaggggctc aaagtctttc ctacacttta gatcaagaac tttctcacac tgaaaaagcc
    21301 actctgcaga ttcaagaaaa aaaagaaatt gctgccacag cagcctcctt gattcacgag
    21361 ggagaaacga ttttcttagg accaggtaca acaatcgaac tattggcgac gcatttactg
    21421 aacaagaaaa ttcggatcat tacaaataat tatccagttt ttgatattct tgctcaagca
    21481 gatggaccag aaattctact gattggcggt gatttccgga aaaataccgg cgcatttgtc
    21541 ggaccgatca ccaacgacaa tttaagaaga ctaaagttta caaaagcatt tatcagttca
    21601 aatggcgttc acaacgaagc gatctctact tacagtaccg aagaaggaga agcacagcag
    21661 atcgctctaa ataattcccg taagaaatat ctactcgttg ataataaaaa attcaatcga
    21721 gatgattttt atgtgtttta taatttgcat gatttcgatc tgctcattac ggatcgccag
    21781 atctcgcggg aggttcaaga gcactatgca caatatgtcg atctgcaaat tgctgattca
    21841 accgaaaata ccactatagg agcgtaacta tgaaagggat tatatttgat ttcaacggaa
    21901 caatgttttt ggactctcat ctgcatgaag ctgcttggct gcatatgata catgatcata
    21961 caaaagatgg tttatcagac gaggatattt taaaaaatat tcatggtcgg acgaatacgg
    22021 agattttgaa acatttcatt tcagagcatt tgaccgcctc tgagatcgcc gtattgtcag
    22081 aagaaaaaga aagctattat cgtatgcttt gcttgcaaaa tgaaaacgag cttcagttga
    22141 caaaagggct tgaagcgact cttgaccagc tgactgacca acagattcca ttaacgattg
    22201 ccacggctac tgtcaaagag aatgttgctt tttattttga tattttcgat ttagcccgct
    22261 ggtttgactg ggacaagatt gtctatgacg acggctcttt ccctgggaaa ccgcaaccgg
    22321 atatttttct taaagcggca gacaaactga gcttagctcc ggctgattgt cttgttatcg
    22381 aagatgctta ctcaggatta cttgctgcca aacgtgcggg gatcggaaca attattgcaa
    22441 ttgatccatt cggtaaaaac aaaacagtat ttgaacaggc acagctaaat aaagatggaa
    22501 ccatcaaaga ttttactggt ttttggacag agcacctgtc tgccacatcc gttaattctt
    22561 aaacgattca actaactacc aaagaaaagc aactgaaatg cacacctcct tttataaaaa
    22621 actgccagtg aaacgacatg aaagatacac tgctatatcc ttagtgaaat atagcagtgt
    22681 atctttgact ttttcattct tttaaccata tgacgcgttc atctctatct ataattaaca
    22741 ttaaaaatag tgttttcaca ttatttctca aaccaatctt aaaaatcatc aggagtactt
    22801 ttttgatata cttttattaa tgctgggcgt aatggttaaa agatttccac tagttcctct
    22861 ccaccaagct gagttgaaaa atgcttatct gtaaacaaca tagacaaaga ggaaataaac
    22921 gtttccgtat ctttctctgt cccaaggata ttaattaatt ttcctactag gtaagtatca
    22981 ttaaacttaa gatctgcctt cacatcaaaa gttttgataa aagcttttat catgcgtaca
    23041 attttgtctt tatcgccata attgatttca ccactcatat caatatagtc actaatccct
    23101 gaagaaataa tggcaactgc ttcttctttc gccttatcag ataattcatt taatgactgg
    23161 gattgattga tgacttcttg agttttatct gttacgtaat ctctcattat cctatcggca
    23221 tactccgcaa agtaatcttg aaaaaattct tctacagggt aaccaacttc gttttcccaa
    23281 ccaggagaaa tttcattatc agtacttatt aaatctatta aaaaccaacc cgttctctga
    23341 gtgttgattc tataatattt ttaatttcct atataaatca aaaatattat agaatcaata
    23401 agctaattaa gaaacaaact ttagaaactc tagaagaa
//
LOCUS       DAYYKN010000036        23293 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000036 DAYYKN010000000
VERSION     DAYYKN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.036"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..719
                     /locus_tag="ACHBB7_001166"
     CDS             <1..719
                     /locus_tag="ACHBB7_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9209128.1"
                     /translation="DQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRNKRLVKVY
                     QTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFI
                     GTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILA
                     VGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGF
                     VLLMITNTIARRFDSDSALF"
     gene            731..1651
                     /locus_tag="ACHBB7_001167"
     CDS             731..1651
                     /locus_tag="ACHBB7_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9209129.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1692..3149
                     /locus_tag="ACHBB7_001168"
     CDS             1692..3149
                     /locus_tag="ACHBB7_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9209130.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3494..4108
                     /locus_tag="ACHBB7_001169"
     CDS             3494..4108
                     /locus_tag="ACHBB7_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9209131.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4101..5825
                     /locus_tag="ACHBB7_001170"
     CDS             4101..5825
                     /locus_tag="ACHBB7_001170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9209132.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5822..7273
                     /locus_tag="ACHBB7_001171"
     CDS             5822..7273
                     /locus_tag="ACHBB7_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9209133.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7745..9898)
                     /locus_tag="ACHBB7_001172"
     CDS             complement(7745..9898)
                     /locus_tag="ACHBB7_001172"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM9209134.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9900..10946)
                     /locus_tag="ACHBB7_001173"
     CDS             complement(9900..10946)
                     /locus_tag="ACHBB7_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9209135.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11096..12385
                     /locus_tag="ACHBB7_001174"
     CDS             11096..12385
                     /locus_tag="ACHBB7_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM9209136.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12398..15100
                     /locus_tag="ACHBB7_001175"
     CDS             12398..15100
                     /locus_tag="ACHBB7_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM9209137.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15085..15942
                     /locus_tag="ACHBB7_001176"
     CDS             15085..15942
                     /locus_tag="ACHBB7_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9209138.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(16013..16870)
                     /locus_tag="ACHBB7_001177"
     CDS             complement(16013..16870)
                     /locus_tag="ACHBB7_001177"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9209139.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            17081..19252
                     /locus_tag="ACHBB7_001178"
     CDS             17081..19252
                     /locus_tag="ACHBB7_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM9209140.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19320..19661
                     /locus_tag="ACHBB7_001179"
     CDS             19320..19661
                     /locus_tag="ACHBB7_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9209141.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19937..21691)
                     /locus_tag="ACHBB7_001180"
     CDS             complement(19937..21691)
                     /locus_tag="ACHBB7_001180"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209142.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21702..22538)
                     /locus_tag="ACHBB7_001181"
     CDS             complement(21702..22538)
                     /locus_tag="ACHBB7_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9209143.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22540..23223)
                     /locus_tag="ACHBB7_001182"
     CDS             complement(22540..23223)
                     /locus_tag="ACHBB7_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM9209144.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT         7659 a         4165 c         4785 g         6684 t
ORIGIN      
        1 cggatcaagc atttctcatc actaaaaata caattttata taatgtagca tttatccttt
       61 tgaatctgct gatctcagtc gtatttgcaa tcattatgag cgagttgaga aataaacgac
      121 tcgttaaagt ttatcaaacg atgtccttac taccttattt tctatcttgg gtcattatca
      181 gttactttgt ttacgctttt ttaagtcctg acaaaggaat attcaatcag tggatcactg
      241 gacatggagg agaagctgtc aattggtata atgaaccgaa atattggccg tttatcttgg
      301 tgttcattgg tacatggaaa gggattggtt ataacagcat tatttacttt gcatctgtta
      361 tgggaatcga tccgacttat tatgaagctg ctatggttga tggagctagc aaatggcagc
      421 agatcaaaca tgtgacgata ccacaactta ttccactaat gacgatttta acgattttag
      481 ccgtaggaaa cattttccga gctgattttg gcctgtttta taacattcct aggaattccg
      541 gtgcattata tgatgtgact caagttttgg atacgtatat ctacaatggc ctgacatcca
      601 caggagattt tggtatgacg gctgctgcag gactttacca atcagttgtc ggctttgtct
      661 tgttgatgat cacaaatacg atcgcccgac gttttgatag cgattctgca ttattctaag
      721 gaggaaaaaa atgaaaaaaa agaaagtgga gagagtccaa gtccgctctt tcaataagac
      781 ggctaatttt ttctctaata taatgattgc actctttgct atttcctgta ttttgccatt
      841 tatatttgtt atcgccatct cttttaccga tgagtccata ttgtcgactc acgggtacag
      901 tttttggcca gaaaagttca gtatatttgg ttatacgtac ttgttcgacc agatgcagga
      961 taaaattttc caagcgcttt tcgtcactgt acttgttact gttttaggta cactgatcaa
     1021 tagcacggct acttctttat atgcttatgc gatttcacgg tcaaactttc cattccgtcg
     1081 tttttttacc gttttttgtt tgattacgat gttgttctca ccagggatgg tggccaatta
     1141 tcttgttatg acaaatcttc ttcagttaaa agatacgatt tgggcattga tcttgccaat
     1201 ggctgtcagt cccttcaata ttattgtgat gcgcacattc tttaaacgtt cggtctctga
     1261 ttcgattatt gaatcggcac ggatcgatgg agctagcgaa ctgcgaatat ttgttcagat
     1321 cgttttaccg ttagctgttc caggtattgc gacaatcagt ttatttgccg cattgggtta
     1381 ctggaatgac tggttcaatg ctttactata catccaagat gataagctag ttccactaca
     1441 gtatcttttg atgaaaatcc aaagtaatat tcaatatctg acacaaaatt caggagcagg
     1501 aagccaattg acaggcggat tagcctcagt tccaggagaa tctgccagaa tggcgatcgt
     1561 agtcatctcg acgttgccta ttgcgatcag ctatccattt ttccagaagc attttgtcaa
     1621 aggtctaaca atcggcggag taaaagaata aagaaacaaa aatataaatc ataaaattag
     1681 gaggaagaaa aatgaagtta tggaagaaac tagcctttac aggtgtttca gcgttgttgt
     1741 tgggaacatt agcagcatgc ggaggttctg gttctaaaga ccaggcagaa gctagcaatt
     1801 cggacacttt gcagatgtat caaatcggag ataaaccaga taatttcgat caattgatgg
     1861 aagtagcaaa caaacgaatc gaaaaagaga tcggtgtaaa agtcaatatc aattacattg
     1921 gctggggcga ttacgaaaag aaaatgaacg tcatcatttc ttccggggaa aattatgata
     1981 tcgcctttgc gaacaactat gtttccaatg cccaaaaagg agcctttgct gatttgactg
     2041 aattagcgcc taagtacgct gaaaaagctt ataatgattt agatgaagca tatattaaag
     2101 gaaatctggt caatggaaaa ttatatgctt tcccagtcaa tggaaatgtc tttgctcaac
     2161 aagtacttac ttttaacaaa gctttactag ataaatacga tctttcaatt gatggaatcc
     2221 aatcttacgc tgatgcagaa aaagtcttga aggaattcca tgaaaaagaa ccaaacacgg
     2281 cagcttttgc cattggacaa ggcttcaaag tccaagggaa ctttgattat cctttaggga
     2341 atacgttacc atttgctatt gatctggatg gagatgattc gaaaatcatc aaccaatacg
     2401 ataatgaaac gtatatcgga ctgttacgta caatgcatag ctggtacaaa caaggactca
     2461 ttccaagtga cgctgcaacg agcaataagg attatccact agaaggaaat acttggctaa
     2521 tgcgtggaga aacacaagga ccgtatgatt atggcgatac aattttgaca aatgcagcaa
     2581 aacaagaact tgtctcaaaa gcaattactg ttccactaaa atctacagct caagcacaaa
     2641 tggcaaattt tgtcgtatca aatacttcaa aaaataaaga aaaatcagta gaacttctag
     2701 ggctgcttaa cagtgatcca gaattattga atgggcttgt ttgggggatc gagggagaag
     2761 catgggaaaa agtcgatggt tcagatgata aaatcaaatt gctagatggt tatcagccaa
     2821 atacacatat gtctgcttgg aatacaggaa acaacaagat tctatataca caagagtcta
     2881 ttacggatga catgattgcc aaacgtgatc aatcgattgc tgatgcagaa acttcaccga
     2941 ttttaggatt cagcttcaac actgacagtg tcaaaacaga attaagtaat atttctaatg
     3001 tcatgaatca atatttagat ggattgaata cagggactgt tgatccagat gaaacattac
     3061 cgaaattgaa agacgctttg gacaaggctg gctatgataa ggtattgaaa gaaatgcaaa
     3121 aacaatatga tgagttccga caagaataat aatgaataaa gacaaagggg ttgtgacaaa
     3181 agacgtgtca caacctcttg ttccgaataa acggtgttca aaagccagtt tctcggtatt
     3241 aagctaaata tatgtaaaaa tgtggaaagc catattcaca aaatttttct tactactcga
     3301 aactaagcgg ctatttcaca aactcttggt aaacggtgtt caatcagcta accctcggta
     3361 ttcgctcaca aaacgacaaa atatgaggaa ctatttttcc gtttgtttct ctaataatca
     3421 ggtcaaggtg ctgattgccc aacctttttt tgtataatga gaaagtataa acttaatgtt
     3481 tggaggagaa gatatgttag ggaaggtttt ggaagagtta tttatccgga tctgggtcat
     3541 tataaaacta accttgtatt tctggatata tacatttgcc ggcgggatca tctttggatt
     3601 aggtgcggca tggaaaacag tcaatgaact attttatttg tacggattcg agtataaaga
     3661 aatcacaata aaaagaggct ggaatatata taaaagaaat tttttaagag gcaatctgct
     3721 atttagttta tttttatctg gtactgcttt attgagttac aatttatatt tatctgttca
     3781 aatcaaaggg ctcatttttt tagctattga ttttatttta ctttttgcct tgctctgcct
     3841 atttgctact tatcaataca gtatgatttt agatagtgaa tatacgatct ctgttcctaa
     3901 tctgctaaag ctaagtttta tttcggtttt ttccagcttt tcgtcttttc taaaaataat
     3961 tattggtagc ggtataattt taggcgtgac ttggcaattc aaaggattga tcctgtttgg
     4021 tgtcattggt cttttgacgg tttggaatgg tacgatgaca acacattggc gtgaagaatt
     4081 agataaacag ttggaaagtt atgaataagt ttcggattct attttcaaaa ggaaccttac
     4141 taaatcgtct gttgcggaaa tacgcggtta tcatgatcgg tgtaacgatg actgctaccg
     4201 ttatttttag tatccatact tgggaacaaa atcaaaaaca ggcagaaaac atgacaagtg
     4261 atgccgttca aagcacaagc agaatgctga atgataaaac aaccttgagt aggatcatca
     4321 aaaaccagct tgtagggaat tccgaaaaga tcgaaaatgt gacgacctac ttaacaaaac
     4381 ccatagatca gtatctgatg tatgtatacg aacagcaaaa cagtaccgat gaattagttt
     4441 ctttcccaaa tcaaatcaaa gatctctacg caaattatga agaactaagt gcgatatata
     4501 tcgtgttgaa tcaactaccg gagtattatg aatcttctag agaaaacaaa ggtggggaaa
     4561 taaaaaaggg gacaccaaaa ttactagacg ccttttatat caagttgcct atcacacaag
     4621 gaggagctga gtcaggaagt atttttattg gttttcaaaa gaaagaacta gatatgattt
     4681 tagaaaatct aacgtcattc aatggactct ctttatacat gatttctgga acgacaaatc
     4741 gactatacac attccatgat aataaaatag ctaaagaaac gtttgaaaga caagaaatga
     4801 tcatcacaga gagtctgaag aaaaattcac aattgccgat tgacgatctg gaaaaaaaca
     4861 attttgttca acaccaagaa ctgtctgatg attatcgtat attagctgta ctagataaaa
     4921 aaagtgtaaa aatcgaaacg ggaaaaaatc ttgccccgct gatcattggt atcattattt
     4981 tagacaatat actgcttatc tttctttata aaacattcaa acgatattca cagcaagtat
     5041 ctatcgtcat gcaggccatg gatcaaacag cagcagggaa tttagagacg cggatcgaca
     5101 taacaaacac ggaatatgaa ctgaaggaac tttcaatcgg tatcaacgaa atgttggata
     5161 gcatcaatca gtttgtagaa gacatctaca aattagaaat caaacagcaa gacgcacata
     5221 tgcgggcgct tcaagcacag atcaatccac actttttata taatacatta gaatatatcc
     5281 gaatgtatgc aatcagcgaa ggaagtgaag aattagcaga tgttgtgtat gcgttttccg
     5341 ctttgcttcg aaacaatacg aatcaagaga aaacgatcac tttaaaagaa gaactggact
     5401 tttgcgaaaa atatgtctat ttgtatcaaa tgcgttatcc taatcgagta gcctatcatt
     5461 ttatgatcga tcctgatttg gaaaagatcg aagtacctaa atttgtgatc cagccattag
     5521 ttgaaaatta ttttaaacac ggtattgatt ttactcgatt cgataatgcg ctaagtgtca
     5581 aagtcttgca ggaaggcaaa cgggtaagga tcatcatcaa agataatgga aaagggatga
     5641 ctgaaaaacg gctaaaacag attgaagaaa aactgtctca tcctaaagtg gaacttcacg
     5701 gttctatcgg tctgcaaaac gtgaatgaac gtttacgtgc cagcttcggc agcagttatt
     5761 atatgtcgct tgagaataat gagacaggtg gactgaccgt ttctattaca ttcaaggagg
     5821 gatgagcgtg tatcaagtgt tattggtaga tgatgaatac atgattttga atgggttaaa
     5881 aaagataatt gattggcaaa gtttaggctt tcaaatagta gcaacagcag agaatgccaa
     5941 agaaggacta gctgtacttg aacagcgaca gattgattta gtagtaacgg atgtgacaat
     6001 gccagaaata aatggactgg aatttattga agcggctcaa aaagaacgtc ataattttga
     6061 atttatgatt ttatcaggat accaagaatt cgattatttg aaaggaggta tgcaattagg
     6121 cgccgtgaat tatttgatga aacccgtgaa taagttcgaa ttggttgaaa gcctcaagaa
     6181 aatcaaaaca cggttagatc agcaaaatga acaaaaaaat caacaagaaa tctatcaaga
     6241 actgttattc agtcaatggt tgaatgaaga attggatgaa ataagtgaag aagaaataat
     6301 aggacaaata agtgagaagc agcataggat agtattgatt caactggcaa gaactcatga
     6361 tactcttatc aatagatggt tgcaagaacg gcaacaaatc ttttactatc aacgaaatta
     6421 cggagaccgt gtattgatca cgttgttgtt ggaggcagat gaagttgatg gcttctgttg
     6481 ttttgttcaa gaaaactttc ctgatcagga atggctgatc agtatcgggg aagagacaga
     6541 ttctttggac aaaatccctg aaacattcca acaagcgaaa gacaatctac agttgcatca
     6601 attttatgga gataaagacg agcatgtttt ttacgcagta caagcagaaa caaaagatca
     6661 aaccatcgat ttttcaagtt tcagacgcgt gctgcaaaat aaacaattgg aagtcgctca
     6721 aaaaatgatc actgattttt ttgaacagtt tcagttagca gccatgatgc cggaagatat
     6781 acgatattcc agttttttac tgtttatgga gattcaacgg gaattgattg atctggaaga
     6841 tgaggaatat ttacaaggaa tcgaaaaaat ccatcaggca aaaacggtgc aagaacttca
     6901 gcagctactt ctgtcattca tcaaaaagca tcagagacag aaaaagtaca gtgacaatgt
     6961 ggaaaaagta atcgtaatcc tgcaccagca ttatcaagaa ccacttacac tcaaagaagt
     7021 gtcagaaaac ctgcatttaa atgtcatgta tctaggacag ctgttcaaaa aagaaacgaa
     7081 aaaaagtttt tcagcttatt tgaaccattt acgtatggaa aaagcgaaac aactgctttt
     7141 gcacagtaat caaaacatca atgagatagc aagtgagatt ggctacaaca acaccacgta
     7201 tttctctaaa ttattcaaga aaatcgtcgg cagatcccct aaagagtacc gagaaaacag
     7261 tggagaatca taaaaaaaaa gccaatgtaa tgaaataact tataaaaaat tgtctagaaa
     7321 tctaattttg ggctgttctt attacttgag gcaaggcagc tttttcatga cctctcgata
     7381 aacggagttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtggaaag
     7441 ccattttcac aaaatttttc ttaatactcg aaactaaaca gctttttcac gacctctcca
     7501 ctaaatggtg ttcaatcagc tgaccctcgg tattagctca aaaaatggca aaatatgagg
     7561 aactattttg ccatttgtct ctctaatact caggtcagaa cgctgatttc acaacctctc
     7621 aataaacggt gttcaaaagc ttgcatctgc ggtaataaga ccaaccaaac gaaaaatatg
     7681 aggatctatt tttgcttggc tgttcttatt acttgatgca ggacagcttt ttcacaacct
     7741 cttgttaatt atagtttgat aaggaaaaag gcaaatcttt tgcttgatat ttcttagggt
     7801 gagggatgat ccctaattgg taagagatcg tgccgccttt cagcaaatct tgatgggtaa
     7861 aatataattt cgtatattct ctgtccatcc gtttgatttt atggataaat tgttgctgcg
     7921 gccgattagg cgttgcttca atcgtcaatt cctttccatt tgacaaaagc aaaacagcct
     7981 tgtcaattaa tggcataccg ataacatatt caccggttcc aggcgtgaca ggataaaatc
     8041 ccagactatt gaagatatac caagcggcca tacttccatt gtcctcatct ccagggtaac
     8101 ccattggaga atcgtcgaag gtctgagtca tcaactgttt aagcagtggc tgtgccattt
     8161 caggttgtcc tatataactg aacaagtaag gatagtgaaa acttggctgg tttgaaatag
     8221 ctaattgtcc aaattctatc gcggccatct cactcatttc gtggatctca aaaccatatc
     8281 ctcctgggtc aaacgttggg ggctgattgc acagctcaat gagtttttct ttatatctct
     8341 cactgccacc atattttccg atcagtcctg aaaaatcgtg ataaacagca aagctgcttt
     8401 gccaagcact tccttctgca taatcttgtc cccaacgatt agctgaaaat ggttctctga
     8461 acttgccatc tttatctttt gaacgcataa atcctgactc atagtcaaag atattttgat
     8521 agttcaatga ttgctgctga tatttcttcg tcatttcctc ttcttctagt accttggcaa
     8581 cttggctaat acaaaaatcg ctataagcat agtctaatgt gtgattgact gattcgtggt
     8641 agtgattagg aacatagcca tatgtcaagt aatcttctgt tccttggcgt ccataatttg
     8701 gatgtttgct ttgaaccgta gcaccttttt ccattgccgt tagaaattca ggcatcaagt
     8761 ctcgtcggat atcttttaca gcagcatctg caatgaccgc atcgatcaat gtcccaggca
     8821 taagtccacg ttcatcagga gacagccatt ttggcaaata gccactttct ctatagctgt
     8881 tcaaaaaacc ttccagcatt tcttcgtatt tttctacagc aatcaaagaa taaagaggat
     8941 aaaccgtttt atacgtatcc caaaacccat tatttgtata gagaacacca ggttttatag
     9001 atcgactagt cgtatcatag tggatttttt gcccactagg atcaatttca taaaatgttt
     9061 gtggaaataa aaacacccga tataaattat gataaaacgt ccgcaattgt tgttgatcat
     9121 ggtgtgtgag ctgtatacga tcaaaatagg actgccattg ttcttgactg tgagcaaggt
     9181 aagtctcttt gctttggttt tgttctagtt ccaggttata agctgcttgt tccgagctga
     9241 taaaagatgt cccaaatcgg atagtttgtt tttttgtctg ttcaaaatac aacgggatcg
     9301 agtcgctatt tttgttggaa acagctaact catcaattgt taaaggatat tcaaatctca
     9361 atataaaata aaaagaaaaa tcaggatctt ctgaaccagc aaaatgtgtg atttttcctt
     9421 ccactgtgta aggatctata acaaagagct gatggcgtcc cggaaaggat aaaagtaaac
     9481 cgctgtcatt tttgctgtaa tcaatagata agattccgcc atacatgcta ggtatcaatg
     9541 tagaacagat gccataacgt aattgacaga tcgacagatg agtcgggcaa aatatacttt
     9601 cttctgggcg gtaagaactt tgagcatgga aaagggtgtt ctcctgtagt tttccattga
     9661 tcggcgtaaa cagcatatga ctgaaatcac ccatccaagg acttggttga tgggtaagac
     9721 ggtagccttg aaaaacgcgg tcatcaggat gaaaccacca gctgccgttt tgatccgtag
     9781 tttgcggtgc aaaataattc atcccaaaag gaacgccagt ataaggctgg cagttcccat
     9841 tagagaaatt tggctgattc gctgttgcat gtctagtatc tatttgaccg atattcattt
     9901 tatttctcct ttgttgattg tcttttgatg aatgaaactg gaaatttact atcggatggt
     9961 cgatcttttg tctctaacca agtgaccaaa gcatctcccg caagcttacc taactgttca
    10021 aaatcttgtg cgatggttgt cagtggcggg tcaacaagag ctgctgcttg gatatcatca
    10081 aatccgataa ttgaaaaatc ttcagggata tgataaccat gctgtctcaa gagattcatg
    10141 gtctggatag cgacaacatc gttttcacaa acaaaagcag ttacttcata cttagtgata
    10201 taagaaagca attcatctaa tgtcgcgctt ttgtcatcca gtgaagtgtg gaaagtcaaa
    10261 ccagctttgt ccaatgcttc aaggtaacca aggtaacgct gtcgaacgga ttgtggatga
    10321 gtctgcgtac caaacaaata agcgattttt gaatgtccct gttcgatcaa ataagaggca
    10381 gcaagcgcac cgccttggaa attatcggaa agaacggtag gaaaaggtaa ctcatagatt
    10441 tttttatcta atatgacgac agggaattct ttcaatgaaa gctcaaaaag taagtcaaga
    10501 tgttgttctg tatcaagagc ataataaatc aatccgtcaa attcattgat tagctctttt
    10561 gctgttttat tttgtagaaa gtcaagcgtc gtcatcatga catcaatctg tttatcttgc
    10621 atcacagggt tcaatccctt gctaaaatca ccaacagaca aatcagcgac atatggaaga
    10681 agaaacagaa tccgtcgtgt ttttgtagga gaagcaagaa tctgttccgg agaagcggat
    10741 ttaacgaaac tgcctttccc gcgaacgcga taaataagtc ctaattgctc taattcagtc
    10801 aatgcacgtt tggaagtgat tctgcttact ttataggtac gtgaaagttc tttttcagtt
    10861 ggtagttggc tgtctacagg taattcacca gttaaaatag ccgtttgcaa atcattaaat
    10921 attgtctggt acaatggttg agtcatataa acacctctta atgatatatc atattcaaat
    10981 aataccataa tggatgaaaa agtaaataaa aagatatttt ttgaaagcgt agacataatt
    11041 ggctgtatga agtataatct ttaaagatat atcaaataga aagaaggttt tattagtggt
    11101 ttataaagat ataccaaaat cggtgcagaa atttatggaa gatatcacac aaaaatgtgg
    11161 ggatatccat gcggactggg caaagaattt tacagcagca tttgccaata ctctgttgac
    11221 aacagtcaaa aagcaaaatg acggaaccac ctttttattg acgggagaca ttccagcgat
    11281 gtggttaaga gactctactg cacaagttcg tccttattta gtgattgcaa aagaagatga
    11341 ggaattagct gacatgatca gcgggttagt caaaaagcaa tttttttata tcaatatcga
    11401 tccatatgcg aatgccttca acgaagaagc taacggtgct ggccaccaaa cagatcatac
    11461 aaaaatgaac gattggatct gggaaagaaa atatgaaatt gattctttat gctacccaat
    11521 acagttggcc tatcttcttt acaaaaatac aggaaaaact gatcaattca atcaatcttt
    11581 tattgaaggt atcaaaaaaa tcttggacgt gttcaaaaca gagcaagatc atgaacactc
    11641 tccttataca tttgtacgag acacgacaag aaaagaagat acgctgatca atgacgggcg
    11701 aggtgcaaaa acagctttta caggaatgac atggtctggt ttccgtccta gcgatgatgc
    11761 ttgccgttat ggatatctgg taccatccaa tatgtttgcg gtggtcgtgt taggttatgt
    11821 ggaagaaatt tttcaaacga tactcgatga taaagaaatt gtacagcaaa cacagaaatt
    11881 gaagaaagag atccaagagg ggattcagaa tcacggacga acggttaatc aaaaagggca
    11941 aacgatttat gcatatgaag tagatggaat gggaaacgcc acagtcatgg atgacagtaa
    12001 tgtgcccaat ctgatcgccg caccttactt gggatattgt tctgaacaag aggaagatta
    12061 tctgcaaaca agaagaacat tgttgagtaa agaaaatcca tatttctatg aaggaaagta
    12121 cgcaaaagga attggatcct cccatacgcc agaaaaatac gtttggccga ttgcattagc
    12181 tatggaagga atgacaacgt cagacaaatc tgaaaaagag cggatattga atttacttgt
    12241 agcaactgac gcaggaacac accttatgca cgaaggattc gatgtgaaca atcctgaaaa
    12301 ttatacgaga gaatggtttt cttgggcaaa tatgatgttt tgcgaattag tcatggatta
    12361 ctttgatatt agagtagaaa agtaggaggt atcgtaaatg aaaaagaaag tatatatcat
    12421 ttctcatagc cactgggatc gtgaatggta catggcttac gaacagcatc acatgcgact
    12481 gattgaattg atagacgatc tactagagct atttgaagtt gatccgtcct tcaacagttt
    12541 tcatttggat gggcaaacga ttattttaga tgactatctt caagtaaggc cagaaaaaag
    12601 acaggcaatc caacaagcaa tcaatgaagg aaaattgaga attggaccat tttatatctt
    12661 gcaagatgat tttcttatca gctcggaatc gaacgtgaga aatatgctga ttggcatgga
    12721 agaaagccgt aaatggggaa cccctgtcat gttaggctat tttcccgata catttggaaa
    12781 tatgggacag acgcctcaat taatgaaaca agctggtatt tcagcagcag cgtttggccg
    12841 tggtgtaaaa ccaattggct ttgataatca agttttggaa gcagaaaact attcttccca
    12901 atactcagag atgtggtgga aaggccctga tcaaacagca atctttggtc tgctatttgc
    12961 caattggtat agcaacggga atgagattcc agtagaaaaa gaagcagctc ttgccttttg
    13021 gaagcaaaaa ttagcggatg cagaacaata tgcctctacg aaccacttat tgatgatgaa
    13081 tggtgttgat catcaaccgg tacaaaaaga tatatcaaaa gcaatccatc tcgcaaatga
    13141 actgtttcca gattacgagt ttattcattc taattttact gactatctgg aagcagttca
    13201 aaaagatgtg ccagaagact taggctcagt agaaggagaa ttgacaagtc aagaaacgga
    13261 tggctggtat actttagcca atacagcatc tgctcgggtt tacttaaaac aatggaatac
    13321 caaggtccag cgacagctag aaaatattac agaaccactt gcgacgatgg cttatgaagt
    13381 tagtggaaac tatccccatg atcaattaga ttatgcttgg aagacattaa tgcaaaatca
    13441 tcctcacgac agtatttgcg gttgttcagt cgattctgtc catcgggaaa tgatacctcg
    13501 ttttgaaaaa gcagatgaag ttggaaagta tcttgcacaa gattccttag aacagctgac
    13561 cgcagcaatc gatacgaccg gattcccaaa agacagcttt ccatttgtca ttgtaaatac
    13621 tgcaggaatg gataaaactg gtgaagcaga aataaccatc gaattagaaa gaaaaagatt
    13681 cgctgaagga atacctgaac aactatatca agagttagaa aatttgccta aacgtaagta
    13741 tcatgtagaa acaaaaagtg gggccactat accagctatt ttatctgaag aaactgtcca
    13801 atttggttat gatttgccga aagaccgttt ccgtgtgcca tatatggcaa gaatgatcaa
    13861 agtgacttta ccattagaaa acatgccagc attttcttgg gaaacattcg cgcttgtaga
    13921 aggggaagcg aaagcagaag aaaaggaaac aatgattcat cagagtggac gaatcatcga
    13981 aaacggccct ctacatttga cgattgaaaa aaatggtacc attacaatgg aagatcgcaa
    14041 aaacaaacga aagttgaata atctgcatat ttttgaagat atcggcgata taggaaatga
    14101 gtatatcttt aaacaacctt tctgtgatca gccaatccta tccagtaaca aagaaaactc
    14161 tgaagtaaaa gtattagtag atacaccgga aatagcaaaa ataagtattc tacaggaaat
    14221 ggaaatccct gtttctgcag acgagcgttt agaaaaagaa cagcagatgg ttgtggaatt
    14281 tcgttatcga aaagcagaac gatccaaaga aaaacgaatc cttcaaataa aaacaatcat
    14341 gacaatcaga aaagatagta aaaaaatcga ctttgaaacg actctcgaca atcagatgaa
    14401 agatcatcgc ttacgtgtct tgtttcctac aaagttacat gtggaacatc acgaagcaga
    14461 tagtattttt gaagtggtga agcgcccaaa ccatgtgtct aaaagttggg aaaaccccac
    14521 gaatccgcaa catcagcaag catttgtcaa tattcatgat gaagagtacg gtgtaacagt
    14581 cggaaacttt ggtttgaatg aatatgaagt aacagaagac ggacaaatcg cagtgacgct
    14641 acttcgcagt gtgggagaac taggcgattg gggatatttt ccaacaccag aagcacagtg
    14701 tctaggtgag catcggttca attacagcat tgaactacat ggaccagaag aaaaattctc
    14761 tacttatttg catgcatacg cagcacagat tcctttttcc actcaacaaa tcaaacatca
    14821 tgaaggaaca ttgatttcaa aacaacaata tttaacgata aaaagtgaaa cgtttgcaat
    14881 tacagctttg aaacgcagca agtttagtga taaagtagta gtaagaggtt tcaatatgtc
    14941 tagtcattta gaaaaattgg aaataactaa agacaatgga aaaactgtca tattgaatct
    15001 gcttgaagaa ccaacgaagc aagcggttgt cccaataatc caaccttatg agattcggac
    15061 aatcggattc gaggaggaaa acgaatgtat ggcggaatag aagcaggcgg aacaaaattt
    15121 gtgtgtgcgg tttcagatgg tggagaaatt ttagaaaaat taagtatccc taccacaaca
    15181 ccagaagaaa cattggatca ggtatttgaa ttttttgatc gttatgaatt gaagggaatc
    15241 gggattggct cattcggccc gattggtatc gacaaaacaa aagataatta cggatacgtt
    15301 ttggcaactc ccaaaaaagg gtgggcacaa ttcgattttc ttggcacaat caaggaacgc
    15361 tacaatattc ctgctgcttg gacaacggat gtcaatgctg ctgcatatgg ggaattgaag
    15421 caaggagcag cacaaggaaa aaatagttgt atttacctta ccgtgggaac aggaatcggt
    15481 gcaggtgtcg tgatcaatga agaaattttt tcagggatcg cacatcctga aatgggacat
    15541 atttgggtga aacgccatcc agatgaccat tatgaaggta cttgtcctta tcataaagat
    15601 tgtttagaag gattagcagc aggaccgtct attgaggcac gcacagggat caaagggcaa
    15661 gatctgccgc aagatcatcc tgtttgggct attcaggctt attatattgc tcaagcactg
    15721 atcaactata cgttgacgtt agcacctgaa aagattattt taggcggtgg ggtaatgaat
    15781 caggatcatc tccttcaaaa aatccgtcag caatttgttg aattgatggg tggttatatg
    15841 gaaacacctc aagtaagtga atatatcgtt cgttggggac tgccgaatga aagtgggatt
    15901 atcggtagtt tgctgttagc agaacacgaa catcaaaagt aagcagacaa attacacaaa
    15961 aagaacagag tcttatcatt atctgctgat aagactctgt tcttttttat ttctattccc
    16021 ataatccagg taaagcttta ccgactattt tttcttgagt aaatggatgg atcatgtgca
    16081 gttctaatgc atggagcatc agtcgttcag ctgttctatt ttggtagaga gggtctccaa
    16141 cgatcggatg tcccttatga gctaaatgaa ctctgatctg gtgtgtacgt ccggtttcca
    16201 aaacacaata gacttgtgtt tgttcagaaa gtactttgtt gacagtgaca tgtgtcaagg
    16261 cgctctgacc atttttatca tctacgaccc tttttctccg atcatggcga tctcgtccaa
    16321 ttttattggt gatggtcata tctttggcaa tttctcccca tacgatggct tgatatctcc
    16381 gatagatcat tttttgttcc aacattcttc caagtatcgg caagacaaat ggattttttg
    16441 caaataaaac ggcaccgctt gtttctttgt ccaagcggtg gacaacgtaa ggtcgttgct
    16501 gtttagtgtc gagataagca gctaaatgat tgagtaaggt actgttttct gtcggttcat
    16561 ttggatgagt cttcattcct gcaggcttat ccactataat caaatgctca tcttcatata
    16621 aaacacggat gtgcttcgca tctcctaatt gaacttcttg ataattataa tctgtttctt
    16681 cgaataacaa tgtgatctgg tcattgccct ttacttcatg atggaacaaa gcaggttgtt
    16741 gattgatcca aacgttttta cgtatacgca aaaagtgtcg aacctttcta ggaactaacc
    16801 attccttttc aagcaagtcg cgaattgttg tttcaggatg gttcataggt aaagtgatcg
    16861 tgattttcac aagtgtactc ctttcagtgc aattgcagac taattgtacc atttttaaaa
    16921 catatttaag aaaaaactta catttttctg ttcattctta tgttactcta cttgcactaa
    16981 cgtaaaaaaa catattgaac tacaacttaa gcaggaagaa aacactcttt cactatggta
    17041 aaataagacg attgaatcaa atagacagga gaagaagcaa atggattttc aaagcatttt
    17101 agaaaagata aaagctgctt tgatcagttt ttggcgttgg atcagaccat atctcggaca
    17161 attccatcga tggcgaaagc gcatctggaa aaaatatcat gtgaacaaat tgattttgct
    17221 gattggtttg atctgtgtct tagtgacaag tatctacctc tttattttag caaaacaagc
    17281 aaatgtcgaa acgttaaaat caggattaag ccaatcgaca gtcatttatg ataagaaaaa
    17341 cgaagaagct ggcacactgt acgcccaaaa aggaacatat gtagaattaa acgcgatttc
    17401 tccctatatc caagatgcag taatctcgac agaagatagg aatttttacg agcatcacgg
    17461 gtttgatatc aaagggattg cccgtgcagc agtccgaatg gtcttgtctg gcggaacatc
    17521 agggggcgga ggaagtacga tcactcagca gcttgccaaa aatgcgtatc tgacattaga
    17581 ccaaacgttt gatcgaaaag cgaaggaatt atttttggca atcgagatcg aaaagaagta
    17641 cagcaaagaa gaaatcttga ccatgtatct taacaatgcc tattttggca acggggtatg
    17701 gggtgtccaa gacgcttcca gaaagtattt tggagtagat gcaacgaatg ttacgctgtc
    17761 tgaggctgct actttggcag gaatgctgaa aggtcctggt atttataatc cgatcgatca
    17821 cccaaaaaat gcgaatgatc ggaaaaacac agtactaagt gtaatggaag aaaacggaaa
    17881 aatcacaaaa gaacaagaac aagcagaaag ccagacagat atcagcgcgt atcttaatga
    17941 cacatacgat aattcagatt ctggttatcg ttatccttat tattttgatg cagtgatcga
    18001 tgaagcagtt aacgagtatg gtcttgatga agaagatgtc atgaataaag gctataaaat
    18061 ctatacagct ttagatcagg attatcaaaa gcaattagaa gctacttatc aaaacagtgc
    18121 attattccca gcaaatgctt cagatggtgc catggtccaa tctgcttcag tagctttaga
    18181 tccgaataca ggtggtgtac aagcactagt aggacgaaga ggagatcatg tctttagagg
    18241 attcagcttt gctacacaga tgcgtcgttc accaggatca acgatcaaac cattaagcgt
    18301 atatacacca gcactggaag ctggatacaa gccttccagt atcttgaaag acgaaccgca
    18361 aagttattac gatgcgcata attttgacgg gacataccaa ggagaagtac cgatgtatca
    18421 agctgttgca caaagtttga atttgcctgc tgtatggctt ttgcatgaga tcggtttaca
    18481 aaaaggatat gataaagcag aagaattcgg cctgccttta gcaaaatctg ataagtatta
    18541 tggcttagcc cttggtggac tggaaaaagg agtatctcca ttgatcatgg cgggtgctta
    18601 cagtgcattt gccaatgatg gtcagatgta tacgccgcat cttatcacga aaatcgttga
    18661 ttcgactgga gcggtgatcg tcgataacac aaacaaaaaa ccaaaacagg tcatatcaaa
    18721 ggaaacagcc gatgaaatga ccagcatgct tcttggtaca ttttcaaatg gaacagcggc
    18781 tgctgcgaat cctgcaggtt atacgatagc agggaaaaca ggaaccacag aaactaattt
    18841 cgatgccaca aaagcaaatg accaatggat gatcggttac acgccagacg tagtgatttc
    18901 gacatggatg ggttacgaag aatccagcaa gctgcactat ttggaaggga caagtggaaa
    18961 tgttgtcggg aaagtcttca aatcagcggc agaaggcatc cttccgtata cttcaaaaac
    19021 aggatttagt gtagcagatg cctatgccac aggaggacaa gtggtagctg cagatcaagt
    19081 tggaaacaca ggaaattcta atggagagtc tggaaattgg caagataact tgaatcaata
    19141 tggtgaacaa gcaaaagaag ggctgaaaaa cttcggcgat atggtgaaag aaggcgttca
    19201 gggaattggt gaagcggcta aagacctctg gcggaaatat caaggagagt agcccctttg
    19261 aaaacgaata accccatgtt acaatagaga gtaactacta aaagaaaaga gggcataaca
    19321 tgtcaaacat ctacgatagc gcgaatcaaa tcgaacgtga gatccgtgaa ttaccagaat
    19381 tcaaagcgtt gaaagaagcg tacgagaaag tacaagcaaa cgaagaagca cacaaactgt
    19441 tcaaagactt ccaagcgatg caaatcgaat tacaagaaaa acaaatgagt ggacaagagt
    19501 tcagcgacga agatgcaatg aaagcacaag aaatggctgt gaaaatccaa tcagaagaag
    19561 taatcaatga cttgatgcaa aaagagcaag gcttcagtac aatcatcaat gacttgaacc
    19621 gtatcatcat gacaccagtt cgcgaccttt acagcgaata aaactatttt atgaaacaac
    19681 agagggtgtg aaagagacgt tcggccttga gcgatgacaa aattgcttct tattgccgat
    19741 aggctgtctc ttgaacacca tttaccaaga ggtcgtgagc ctaccgttta actctgagtt
    19801 acaaggaaca atttcaaaaa acagcttctc atgtttgatg actattgtga cttgtgaccg
    19861 aggagttggc aggagaacac cgtttattca gaaataggag ctacgacaaa agtcttgtca
    19921 tagctcctat tttttgtcat tcatttttca agcggtagaa atagcctttt tgagctaaca
    19981 gtttttcgta agtacctatt tcaacgattt ttccttcatc gatcacaacg attttatctg
    20041 cctctttgat cgttgaaagc cgatgagcca caataaaagt agtgggtgtt tctgtcaggt
    20101 aattcagggc ttgctggatt tttttctctg actggttatc taaagctgag gttgcttcat
    20161 ctagcaaaat gatttttggc tgacgtatca acgctcgtgc aatggagata cattgctttt
    20221 ggccaccaga cagcttgttg ccatgttcgc ctactaaggt atccagacca tcaggaagct
    20281 ggtcaatcag tgaagaaagt tgcgcagctt cgatgacttc atctaaacgt tcttttgata
    20341 cgtttttcaa gccataagtg atgttctctt tgatcgaagc ggagaacaac aaagtggttt
    20401 gaggaacaac ggctaaataa tttcgcacac ttcgcatatc cagttcatcc aaaggctgat
    20461 catctagaaa aatttttcct gaagtcggct ggataaatcc aatcaataag ttaaggagtg
    20521 tagattttcc agagccagat ggcccaacaa aagcaattgt ttctccagct ttcacctcta
    20581 gagaaaaatg atcgataatc ggtttgagtg agtccggata aagaaaagaa acatcctcaa
    20641 aacgaaaatt tccatccaaa tgttggattt ttttcttacc gtgataagtt tctacatcgc
    20701 cagcaatcaa cagttcgcca atagaagtga tagattccat gccttttgaa atcgtaggaa
    20761 tcagcgtgat cattccagaa acggaattga cgatcgtcgt gaaatacgtc tgatacatca
    20821 cgacatcgcc gggttggatg cgcccttgta aagctaatat cccagtgaaa ctcaaacaaa
    20881 tcagctgaaa tatctgaaaa ctagcccagc tgactgatcc gaaaacagat tgcaggatat
    20941 ctaagtggaa tcctttttca tagatttgag taaaatgctt atttaagcga gttgtttcgt
    21001 gtttttctaa cccatgagct cgagcaatcg gtgctaattc gatcacttcg atcacttttg
    21061 cagaagtctc ttccatttct tgcctgaagt ctgagttgga ctgtctgatc cttttacgga
    21121 aaaagagagt aacgaatgcg gcgagaggaa cagtcaataa gaaaaacatg aaaacaacag
    21181 gacttttata aagcgtgata cctaaagcta cgaataaatt gacagcgata tttaatccgc
    21241 tgacaaatac ttgagaggac aacgtttgga tagcttctac atcacggatg atctttgact
    21301 ggattcgtcc agattgcaat tctttttgat aaggaataga tagttcttgg attttcttga
    21361 taagggtcgc tcttagtcca gcttctactt gacggatcga agtgctatgg tattttacat
    21421 gaaaataatt cgtcaatata ttttgaccaa tcagacaagc catcaaaaaa gcgttgaaat
    21481 aaatggcctg caactgagaa gaatctttcg atgaagctgc attgattaca ttggcaatag
    21541 cgatcggcat taaccaagat ggaagatgtt tgataataaa aaagagtcct gacatggcaa
    21601 aacgccaata atttccctga taaagatgca gcaatgtctg aaaagcattt gcatgatatt
    21661 tttgataact tttctgtatt cgttcctcca taatcttctc cttaacaatc ttggtaaata
    21721 aactgcaagt gttctattgc ttcaggaaca taaatctcag gagtagcttc aagagtggcg
    21781 tacattagag gacgctcata tttgatataa tgcagtaccg ggcggaaatc taaacagccc
    21841 ttgccaacag gtaccatgcg gatctttcca ttttccatga tatagtcttt gagatgaagc
    21901 gctgtaatat aaccatgtaa attgtccaag gcacctcgga tcactttttc ctgatcagca
    21961 tgattttctt tatgcatcaa attcgcacaa tctaaaatga tttttaaatt tggtgaagct
    22021 acttcatcga ttaaacgttt agctaactga tagttataga ttggatgatt gatccctgct
    22081 tcaacagcaa cagttacgcc aaaattttct gcatgagcga ctaattcaat gatagaattt
    22141 ctggcgatct gataagcttc ttcagtaaaa ttttcttttg tgtaaccgac agagacacta
    22201 ccggtttccg tagcaatcat cttggcttga taggcagagc acagagcaat ttgctgtttg
    22261 aaagttgcca atgcttgtgc acgaacttct tgatttttgc tggataggtt gatatagcat
    22321 cctaaaatgg aaagttcgat attgtttttt tggaagtatt caccaaaata ggcagcactc
    22381 ccaagtgtca tgtcagttaa tttaagttgt tcaggaaatg ctttttgtgg tgcaaattgg
    22441 atatgggaca agtgaagctg tgatactttt tgaacgattt cttcacgatt tcgaacatct
    22501 aaatcgtggg cacggatgcc taaatttaat ggaaacatct caaactcctt tttgtaaata
    22561 attagcaaga ggcagacggg attcttttat caattcaaca attattcggg caactttatc
    22621 agcgccttct ggactaaagt gggtattgtc ttttagtcct tctgggtaat ttttacaagt
    22681 atcaggcata agatgcaggt agaatttttt tgtctcttct tttgagaagg tctggaacaa
    22741 ttcttgtgat cttgagaaca catctaaaag aggaatatgg tgtttctcag caaatgcacg
    22801 catcgctttt ggatagtctc caagtatatc tggattcaat gttccgtttt ctaagtaatc
    22861 tcgtcgcgtc acagatgtca gtaagaccat ttggacgttc gctttttttg cgcattcaaa
    22921 aaatatctgc agatttttta aatagctacc gaagggaagc gtacctcttt cttcttggat
    22981 tttctggtcg ttatgtccaa attgtacaaa taagaaatca ttcggctgaa actgtgaagc
    23041 tagacgggct aaccttcctt cctggataaa tgatttggtg ctgcgtccat tctttgcttg
    23101 gttattgatc tggatctcgg gtgaaaaata agcagagatt ttttctcccc aacctgtctc
    23161 gggacgcttg ttttcatctt tttctgccgc agtagaatca ccggcaatat ggatagtagg
    23221 catgttatct cttctttcat aaaggtttat aggcaatgaa gcagaacagc aagagaagaa
    23281 gacggagcaa att
//
LOCUS       DAYYKN010000037        23167 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000037 DAYYKN010000000
VERSION     DAYYKN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.037"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACHBB7_001183"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACHBB7_001183"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM9209145.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACHBB7_001184"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACHBB7_001184"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM9209146.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACHBB7_001185"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACHBB7_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM9209147.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACHBB7_001186"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACHBB7_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM9209148.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACHBB7_001187"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACHBB7_001187"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM9209149.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACHBB7_001188"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACHBB7_001188"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM9209150.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ACHBB7_001189"
     CDS             complement(8054..8524)
                     /locus_tag="ACHBB7_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM9209151.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ACHBB7_001190"
     CDS             complement(8570..8995)
                     /locus_tag="ACHBB7_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM9209152.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ACHBB7_001191"
     CDS             complement(9062..9331)
                     /locus_tag="ACHBB7_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM9209153.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ACHBB7_001192"
     CDS             complement(9342..10937)
                     /locus_tag="ACHBB7_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9209154.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACHBB7_001193"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACHBB7_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM9209155.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACHBB7_001194"
     CDS             complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACHBB7_001194"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM9209156.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="ACHBB7_001195"
     CDS             15403..16377
                     /locus_tag="ACHBB7_001195"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM9209157.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="ACHBB7_001196"
     CDS             16547..17947
                     /locus_tag="ACHBB7_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM9209158.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="ACHBB7_001197"
     CDS             complement(18025..18747)
                     /locus_tag="ACHBB7_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9209159.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="ACHBB7_001198"
     CDS             complement(18826..19332)
                     /locus_tag="ACHBB7_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9209160.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="ACHBB7_001199"
     CDS             19455..21254
                     /locus_tag="ACHBB7_001199"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM9209161.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="ACHBB7_001200"
     CDS             complement(21322..22896)
                     /locus_tag="ACHBB7_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM9209162.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6353 a         5026 c         4229 g         7559 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaata
//
LOCUS       DAYYKN010000038        23061 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000038 DAYYKN010000000
VERSION     DAYYKN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.038"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="ACHBB7_001201"
     CDS             complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="ACHBB7_001201"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM9209163.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="ACHBB7_001202"
     CDS             complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="ACHBB7_001202"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9209164.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8128..9096
                     /locus_tag="ACHBB7_001203"
     CDS             8128..9096
                     /locus_tag="ACHBB7_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9209165.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9114..10019
                     /locus_tag="ACHBB7_001204"
     CDS             9114..10019
                     /locus_tag="ACHBB7_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9209166.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10016..10822
                     /locus_tag="ACHBB7_001205"
     CDS             10016..10822
                     /locus_tag="ACHBB7_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209167.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10979..11947
                     /locus_tag="ACHBB7_001206"
     CDS             10979..11947
                     /locus_tag="ACHBB7_001206"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM9209168.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12264..12788)
                     /locus_tag="ACHBB7_001207"
     CDS             complement(12264..12788)
                     /locus_tag="ACHBB7_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM9209169.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13075..14829
                     /gene="scm"
                     /locus_tag="ACHBB7_001208"
     CDS             13075..14829
                     /gene="scm"
                     /locus_tag="ACHBB7_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM9209170.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSS
                     TTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVI
                     LLVIGLRITFMSYKKSKR"
     gene            complement(14984..15970)
                     /locus_tag="ACHBB7_001209"
     CDS             complement(14984..15970)
                     /locus_tag="ACHBB7_001209"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM9209171.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(15993..16559)
                     /locus_tag="ACHBB7_001210"
     CDS             complement(15993..16559)
                     /locus_tag="ACHBB7_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM9209172.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16573..16989)
                     /locus_tag="ACHBB7_001211"
     CDS             complement(16573..16989)
                     /locus_tag="ACHBB7_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM9209173.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17446..19380)
                     /locus_tag="ACHBB7_001212"
     CDS             complement(17446..19380)
                     /locus_tag="ACHBB7_001212"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9209174.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19682..20617
                     /gene="pplA"
                     /locus_tag="ACHBB7_001213"
     CDS             19682..20617
                     /gene="pplA"
                     /locus_tag="ACHBB7_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM9209175.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20707..21810
                     /locus_tag="ACHBB7_001214"
     CDS             20707..21810
                     /locus_tag="ACHBB7_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM9209176.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21823..22776
                     /gene="menA"
                     /locus_tag="ACHBB7_001215"
     CDS             21823..22776
                     /gene="menA"
                     /locus_tag="ACHBB7_001215"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM9209177.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(22846..>23061)
                     /locus_tag="ACHBB7_001216"
     CDS             complement(22846..>23061)
                     /locus_tag="ACHBB7_001216"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209178.1"
                     /translation="YSKLSDFFTTSDKRCSKASFSVLSQIYAKMWKAIFTKFFLILET
                     KRLFHNLLINGVQKLASAVISPTKRKI"
BASE COUNT         6780 a         4869 c         4508 g         6904 t
ORIGIN      
        1 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
       61 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
      121 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
      181 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
      241 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
      301 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
      361 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
      421 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
      481 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
      541 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
      601 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
      661 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
      721 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
      781 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
      841 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
      901 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
      961 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
     1021 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
     1081 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
     1141 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
     1201 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
     1261 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
     1321 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
     1381 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
     1441 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
     1501 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
     1561 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
     1621 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
     1681 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
     1741 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
     1801 tcttttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
     1861 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
     1921 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
     1981 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
     2041 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
     2101 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
     2161 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
     2221 atatcttgag aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
     2281 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
     2341 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
     2401 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
     2461 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
     2521 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
     2581 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
     2641 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
     2701 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
     2761 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
     2821 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
     2881 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
     2941 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
     3001 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
     3061 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
     3121 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
     3181 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
     3241 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
     3301 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
     3361 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
     3421 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
     3481 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
     3541 gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag
     3601 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
     3661 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
     3721 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
     3781 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
     3841 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
     3901 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
     3961 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
     4021 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
     4081 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
     4141 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
     4201 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
     4261 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
     4321 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
     4381 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
     4441 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
     4501 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
     4561 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
     4621 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
     4681 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
     4741 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
     4801 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
     4861 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
     4921 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
     4981 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
     5041 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
     5101 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
     5161 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
     5221 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
     5281 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
     5341 tcaacgacac catcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
     5401 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
     5461 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
     5581 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
     5641 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
     5701 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
     5761 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
     5821 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
     5881 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggcgaa aagacgacgt
     5941 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
     6001 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
     6061 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
     6121 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
     6181 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
     6241 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
     6301 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
     6361 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
     6421 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
     6481 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
     6541 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
     6601 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
     6661 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
     6721 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
     6781 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
     6841 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
     6901 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
     6961 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
     7021 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
     7081 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
     7141 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
     7201 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
     7261 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
     7321 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
     7381 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
     7441 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
     7501 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
     7561 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
     7621 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
     7681 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
     7741 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
     7801 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
     7861 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
     7921 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
     7981 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
     8041 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
     8101 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
     8161 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
     8221 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
     8281 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
     8341 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
     8401 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
     8461 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
     8521 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
     8581 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
     8641 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
     8701 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
     8761 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
     8821 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
     8881 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
     8941 ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
     9001 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
     9061 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
     9121 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
     9181 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
     9241 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
     9301 cttgtcttct aggcttttta gggagtgcgg cagcagggct tgtttccgga ctgcttcaca
     9361 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
     9421 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
     9481 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
     9541 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
     9601 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
     9661 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
     9721 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
     9781 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
     9841 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
     9901 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
     9961 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    10021 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    10081 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    10141 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    10201 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    10261 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    10321 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    10381 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    10441 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    10501 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    10561 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    10621 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    10681 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    10741 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    10801 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    10861 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    10921 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    10981 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    11041 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    11101 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    11161 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    11221 acaaacgatg atgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg
    11281 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    11341 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    11401 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    11461 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    11521 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    11581 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    11641 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    11701 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    11761 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    11821 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    11881 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    11941 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    12001 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    12061 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    12121 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    12181 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    12241 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    12301 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    12361 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    12421 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    12481 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    12541 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    12601 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    12661 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    12721 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    12781 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    12841 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    12901 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    12961 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    13021 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    13081 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    13141 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    13201 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    13261 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    13321 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    13381 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    13441 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    13501 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    13561 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    13621 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    13681 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    13741 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    13801 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    13861 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    13921 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    13981 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    14041 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    14101 acagatacat ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat
    14161 actacctcta ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact
    14221 acctctacca caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc
    14281 tctaccacaa gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct
    14341 accacaagtg atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc
    14401 acaagtgata gcacggacac gtcagcttca agtgacagta cagatactac ctctaccaca
    14461 agtgatagct cagattcatc taccactaca agtgatagca cggacacgtc agcttcaagt
    14521 gacagtacag atacatcctc taccacaagt gatagctcag attcatcaac ttcaagcgat
    14581 agtacagaca cgtcgtctac cacaagcgat acacgtcatg caagtgtctc cttctcaaaa
    14641 acaagttcgg ataacaggac gaactttaac ttaactaata aaacaagcaa tgacaatgga
    14701 aacaaatcaa aaagagcact tcctaaaact ggaagtcaat cgaataattg gataacactc
    14761 gcaggcgtta tattacttgt aatcggttta agaatcactt ttatgagtta taagaaatcg
    14821 aaaaggtaaa aaaatattca agataaaaaa gagcaacgtc gctaacggac gttgctcttt
    14881 atttttgctt tttatcataa atacaagaac tacaattcaa tttgtcaaaa aacacgtaga
    14941 tttccttctg agaaatttat ttacgtatcg aacctttccc tattcagtct ttgcgatcca
    15001 aaatcagttg ggtaagggaa aagagattct cttttgttcg aagagaggtg gcaggtaatt
    15061 tctttatttc cttcaaagcc ttttctgtgt aagaggccgc taacttccta gcttcttcca
    15121 cgcctcctgt atgttcaacg attttgcgta cggcgtcgac atcttcttct gtcattgccg
    15181 ctttcttctc taaataaggt agtagctttg gtttgttttt ttgcaaagag tagatcaacg
    15241 gtaaagaata gaccccttga cgcatgtctt ctagtacagg tttgccgatt tcctggctct
    15301 cttttgtata atctaaaata tcatcgataa tctgaaaggc catcccgata gaaagtccga
    15361 tatctcctgc tttttttgcg aagcgctcgc ttgttccact ttcaaaagcg ccaacagaac
    15421 aacttaattg gaacagttca gctgtcttac ctgaaatatt ttccaaatat tcttttacag
    15481 aaagttcata attatagcga tcatccatct gccctaattc gccattcaat actttttcca
    15541 tactgcgaga attcaattgg agacttttca aggaagtgga gtaatcagac aatagtttaa
    15601 aacagcagac aaataaatag tctcctgcat agacagaaac gacattacca aactgactac
    15661 ggatattagg ctgaccacgt cgcagatccg cttcatcaac gatatcatca tggatcagtg
    15721 tggccgtatg gagcatttcg atagaagctg ccaaagcgat ggctttgtct cgatcttgct
    15781 ctggtccaaa ttgtgagaac aatagctgat aggcaggacg caacagcttt ccgccggcat
    15841 ggatcgtttc tttgattgct tgttcgacag gtttgttttt caggcggaca gtttcttcca
    15901 tccgttttag tgtttctgtc aattctttac ttaaagcagg atagttttcc cacattggat
    15961 gtagcttcat aaaaaaactc cttgtgtgac gattattttt tggtaaacat tgggctccat
    16021 ttttgtttgg ttccctgctc ataatctttt tggaatcgtt gaagtgtctg cacatgtcgt
    16081 aaaagataat tcgctgcgat cccgattgca atccctgata aaatccctaa aactgaaagg
    16141 actggcaagt acaacatgac tgtccatgat tgtgcaatga aagaagccat caaaagctgt
    16201 ccgacattat gcatgaaacc gccggccgca ctaattccga tgatactgac acgttttggt
    16261 cctaattgct tgaccaataa cattccaaaa taactaagca aagcaccact catactataa
    16321 aggaatgtgg aaatcgttcc gcctagcaaa gtagtcagga atagtcgtag ccaaagtagt
    16381 aagaagctgt cttgtttttt catggtaaaa atggcaacga tcgtgattag attggctaag
    16441 cctagttttg caccaggcgc aaaagaaaaa ggataaggga tcatattttc caataaacca
    16501 atgatcactc cttgtgcaac aagtagcgaa atataaatca ttttttgtaa tttactcatg
    16561 gtgtgtaaag gtctaataaa tcagactgtc ttcgtcacct ccatctgttg aacggacttc
    16621 gatcactaat ttatgtggca aacagacgat ggtttcgcca tttttcgttg cccagcctcg
    16681 acgtacgcag atctggtcat cgcaatctgc ttcttttatc cgtactcgat cgccatctat
    16741 ctcgatcaag ttataatctc cgtgttcatc tttgtaagta tatgtgttgc tctcttctcc
    16801 agccatcaaa gggaatgttt tgatttctgt gccatccaca cgcagtaccg cttctttttc
    16861 tgctgttgcg gtgttttgct gaagaccaaa aatcatgact ggtaaaaaag aaagaacagt
    16921 caaaagcaaa ataataaaaa catcccaagg tttgagcaga cttttttcag caaattcttt
    16981 aaggctcatt tttgttctcc tttttccaga ttaactcacc caattttagc ataaaaacaa
    17041 gaggttgtga cacctgtgtc ttgctttgag taataagaac gaagaataga aaacagcttt
    17101 ccatgttttc taattctttg ggcttattac cgagaagcag caggtggaac accgtttata
    17161 acaagaggtt gtgacagaag tgggcaactt caagaaataa gaaggaattt acgaaaatta
    17221 cttttcaagt ttttgtgaaa tttcggctta tttttcgaag aagctacttc tggaacaccg
    17281 tttataacaa gaggttgtaa cagaggttgt gacacctgtg tcttgctcat ggaggcttat
    17341 ctcgggcatc ttattccttt aattttacat aaaaaggagg ctgtgacggt ttgtctcagc
    17401 ccctttctat tgaaatcttg cattcaaata acgttcttta tctatttatt tcttgccgta
    17461 ctgggaactt ggtctaccgt accaccaatt acctagtgta cgttgtagcc atggaattac
    17521 ccagcgatcc aacccaatcg cacgtccgga accgttcatc aatgcgaaag caacaaaaat
    17581 gaaccaaatg tttacccagt aaaacatgcc tgataagcag aacgtgatca ccaatacaat
    17641 cgttgtcgca ctgcatagcc atgtgaataa tccagcaatc aatgctaatg ccaaggcaat
    17701 ttcaaagaat accatgaatt tttggaagaa cattgctaca tcattgtttg gcatgatcac
    17761 ttgcatgatc tttgtgaacc agtctggtga cttctcaatc acagccattg gttcttcacc
    17821 gtatgcataa ctcaaaccaa aatgtgcagc ttgagtcgct gcttctccgg cacttgcccc
    17881 tgctccgctg gcagctgtgg ttgcatcacc agcagcagaa gcaccactgg caacatctgt
    17941 tacattgtat tcccatgtcc aagggaacac actgtttcca gtgaaccatg atccttcacc
    18001 aaaccaagta cttggtttga acacttcacc attcccaatg atttttttgg aagcttcaac
    18061 gaaccaaact aaaccataga aaatacgaag tggcacactc cataagacat tgccgtaacg
    18121 agaagaatgt cctcgagtca cgttgcgatc atctttgata tggaaaaact catgcattaa
    18181 atattggaac atataataac cagaacgaat atcaaagaag tatttcaaat tcacgatatg
    18241 tttcattacg atagctaaaa atccgcttaa atggatctta tcaaataggt tagctacccc
    18301 ccatttggca ccaatagaaa ccataaatcc ttgatagttt cctttaaatt tatgcttttc
    18361 tccgcctttg atcgaagcca cgatattcgc tgcggctgtg tgtcctgttt gttcagcagc
    18421 ttgaacgatc tgcggtgtcg gtgttcctgg gaattcttca taaaagacta aatcaccaat
    18481 gatatagatg tttttatctt cgtagccttt tgcctgcata tattcattgg cgatcaaccg
    18541 ctgtccgcgt gctgattcta atccaaagtc agcagcgtca gaagttcctt tgacaccagc
    18601 tgtccaaatc agtgtgtgag tcggaatgtt gcgtccgtct ttcaatttga tatgatcagc
    18661 agccacttct acgattggtg cattcaataa taattccacg ttgtgcttac gcaaatagcg
    18721 ttcagctttt gcagcatcat ttcgatccaa catattcaag atcgtcggca ttgcctctac
    18781 gaccatcagt gtgatttcgt ttggatctaa tttataatct ttggctaggc gatttttcca
    18841 gtcgatcagt tccccaatca tttcaatacc ggtaaatcca gaaccacaga caacgaaagt
    18901 cagcatggct ttacgcagtt catcatctgg ttccaatgca gcttttgcta ctgtcttctc
    18961 aatatgttcc cgaatcctta cagaatcttc aaatgaccaa agggtgaatc cgtgtgcttt
    19021 gacaccaggt gtaccaaagt cattcggctc tccgcccatt ccaaggatca gctgatcaaa
    19081 aggataggaa cctaatttgg tcttgacgat ttttcgttct ttatcgatac cgattacagt
    19141 gtctgtgaca agttgtacat tcttttgacg agcaaacaaa tgctgcagat cgtattggat
    19201 tgcttctggt tctacacgtc cgccagctac ttcatgcaac tcggtcatca tagtatggta
    19261 cgaatgacga tcgatcagag taattgctac atcttctttt ttcagtttct ttgccaagta
    19321 ttttgtagct gaaacaccgg catatcctgc tcctacaacg acaatatttt gctttgccat
    19381 tgatcatccg ctccttatag actatctata taatgaatat atgataattc ttttagtttg
    19441 atacttatta cacaatctcc atatattata tagatgttct gttcttttgt ctaactcctt
    19501 ttggattttt ttcaaatttt aaaataaagc gttttttcac gaaatttgct gaaagaaaac
    19561 gtttgaaata ttacatgggt tatttatcaa aaatagttga cttataggct aaaaagatta
    19621 gaatgtataa tgaatcacaa agaaaaattg tggactagaa atggaatggt ggggtattaa
    19681 aatgaagaaa aaacgttttg taactggatt tgccgtatta gcattttcag ctctagtgtt
    19741 aggcgcttgc ggcgcagatg acaacggaag ctcaaactct tcttctgaat catctacagc
    19801 acaatcttcg actgcaaaaa gcacaacaga atcatctgca aaagttgtag ctggcggaga
    19861 tttgcaagac ggcacatata aattagaaga aaaaaattat tcaaatggtt accgtgcagt
    19921 ctttgaaatg gttgtaaaag acggcaaaat cactgaatca aaatatgata acgtcaatga
    19981 aaacggcgaa tcaaaaacaa aagatgcaga atacaacaaa aacatggaag caaaatctgg
    20041 cacaaaccca gaaaaataca ttcctgagtt gaatgaccaa ttcttaaaag cacaaagcgc
    20101 aagcggtgtg gaagtcgtaa ctggagctac tcactcatct gaaagcttcc aaaactacgc
    20161 acaacaattg atccaagctg ctcaagctgg taacacagat acaatcgaaa tcgacaatgg
    20221 tgctgatttg aaagacggca cttacaagct tgaagaaaag aactactcta acggctaccg
    20281 tgtgcaattc gaaatgacag ttgctggcgg caaagtaact gaatcaaact ttgattatat
    20341 cgacaaagac ggtaaatcaa aacaagatga tacagaatat aatgaaaata tgaaagcaaa
    20401 aagcggtaca gaaccaaaaa cttacatccc tactctgaac gacgaattcg tcaaagcaat
    20461 gggcgaagaa gatggctcac ctgctaatgt agaagttgtt acaggtgcta cacacagctc
    20521 acattcattc atcatgtacg cacaacaatt agtgaatgca gctgaaaaag gcgacactca
    20581 aacaatcgaa gtagataata tcgtaacaga aaaataatca cataatcata ctaataaaag
    20641 ttggagggtg ggacgatagc cagattaggc agtcgtctca cctttcaaaa tgaagagagg
    20701 aaaaagatgc ggaaaaaact actattttcc ttaatcacac tgggcatctc ttttatggtg
    20761 ctaactggct gcagcaactc agcaaataaa acaaccactg aaagttcagc agctaccaag
    20821 atattgaaag atccatattc tgatgaacag ttcttgttag ggacttacgt tcgtatccgt
    20881 gtgtatgatg aaggcaaaga atcagcattg aaacctgcct tcgatcgagt aaaagaactt
    20941 ggtgataaaa tcacgatcaa tcaaaaaggt tctgagatcg atgaagtgaa tgagcaagct
    21001 gggatcaaac cagtaaaagt ttctgatgac gtctacacgt tagtcaagcg ggcctacgaa
    21061 tatagccaag attctcaagg cggattcgat atggcgattg gtgcaatcac acaattatgg
    21121 cgaatcggtt ttgatgacgc cagaaaacct tcccaagaag aaatcgatca ggcattgaaa
    21181 ttagtggatt atcataagat cgaactaaat gacaaagaaa aaactgttta tctaaaagaa
    21241 aaaggaatga tcatcgattt aggtgcaatc gccaaaggtt acattacgga cgaagtagtg
    21301 aaagtcttga aaaaacaagg agtgaccact gccatcgttg acttgggcgg taatgtctat
    21361 gtcttaggcc acagcccaag aggcgaaaac caagactgga cagtcggcat ccaagatccg
    21421 aacatggctc gcggatctgt tttaggttct attaaagaac ggaacaaaac attagttacc
    21481 tctggaattt acgagcgtta tttagaagta gatggcaaaa aataccatca tttgtttgat
    21541 ccaaaaacgg gttatccgtt cgacaatgat atcgcaagtg tcacgatcat aacagataaa
    21601 tcaattgatg gtgacgggtt gtctacagca gtcttttcaa tgggtgtgaa aaaaggactg
    21661 gaatacgtcg aaagcgaatt gaataacggg acggaagcga tcttcgtgac aaaagacgat
    21721 aaagtatatg tgaccgatcc aatcaaagac accttcaaat tatcggagga ctcccattac
    21781 acgatgggtg accgttctga actaaaataa ggagtacgaa ccatgacact aaaaatcttt
    21841 ttagaagtag ttgaaatacg aacaaaggtg gctagtgtat ttccttttgc ggtaggggta
    21901 ttattctcta tcgctttttt tcatgagata cattggctaa acactttttt attttttatt
    21961 ggaatgctag tttttgattt agcgactacc gctatcaata attacatgga ttatgaaaag
    22021 gctcattctg aagtatataa gtatgaagaa aatgtcattg gccgcagaaa tctatctcct
    22081 gtattgattc gaaacatgat tttagctatg attgcatttg tttttgttat cggcgttatt
    22141 ctaacgatca atacgggttg gcttttactt ttgatgggat ttatctgttg ttttattgga
    22201 gtattctata cattcggacc gattccttta tctcgaatgc ctttagggga aattttcagt
    22261 ggggtgacta tgggccttgg aatttttgcc atggtcgttt atgtcaatac attcggttca
    22321 gatgtctatg atctgacagt caacttttca tctggacaat ttgctttggt aggaaatctt
    22381 tgggctatag cagcaatcgt attagcttcc ttacccttgg tgtttacgat tgccaatatc
    22441 atgttggcta ataatttgcg ggatcttgac cgtgatattg aaaatcatcg ctatacacta
    22501 gttttttata tcggacgtcc tgcgggaaca gtcttattcc agctattgat gtatgcttgc
    22561 tatttcgtga ttttaatggg gttgttgagc ggtatctatg aatggccgat cttggtcacc
    22621 ttcttgacct tgccaattgt gaagaagcat ttggatcaat tcaagcaaac gttaccccag
    22681 cctatcagtt tcagctattc catcaaaaat atggtcttat tcaatgccag ttatgtattg
    22741 gggctattac taacaatcat tatgcaagcc atttaacagc atgaagaggt tgtgaaaaaa
    22801 ctgccctgca tcaagtaata agaacagcca aataaaaata gctccttata tttttcgttt
    22861 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttatcagga ggttgtgaaa
    22921 aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc
    22981 ataaatttgg cttaataccg agaaactggc ttttgaacac cgtttatcag aggttgtgaa
    23041 aaagtcgctt agtttcgagt a
//
LOCUS       DAYYKN010000039        23009 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000039 DAYYKN010000000
VERSION     DAYYKN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.039"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..2606
                     /locus_tag="ACHBB7_001217"
     CDS             135..2606
                     /locus_tag="ACHBB7_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9209179.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="ACHBB7_001218"
     CDS             complement(2709..4445)
                     /locus_tag="ACHBB7_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9209180.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="ACHBB7_001219"
     CDS             complement(4442..5146)
                     /locus_tag="ACHBB7_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9209181.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="ACHBB7_001220"
     CDS             5277..5780
                     /locus_tag="ACHBB7_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM9209182.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="ACHBB7_001221"
     CDS             5740..6078
                     /locus_tag="ACHBB7_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM9209183.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="ACHBB7_001222"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="ACHBB7_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM9209184.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="ACHBB7_001223"
     CDS             7629..8207
                     /locus_tag="ACHBB7_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM9209185.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="ACHBB7_001224"
     CDS             8211..8732
                     /locus_tag="ACHBB7_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM9209186.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="ACHBB7_001225"
     CDS             complement(8811..9365)
                     /locus_tag="ACHBB7_001225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM9209187.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9618..9893
                     /gene="rpsP"
                     /locus_tag="ACHBB7_001226"
     CDS             9618..9893
                     /gene="rpsP"
                     /locus_tag="ACHBB7_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM9209188.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9905..10156
                     /locus_tag="ACHBB7_001227"
     CDS             9905..10156
                     /locus_tag="ACHBB7_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM9209189.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10281..11072
                     /locus_tag="ACHBB7_001228"
     CDS             10281..11072
                     /locus_tag="ACHBB7_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209190.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11242..11763
                     /gene="rimM"
                     /locus_tag="ACHBB7_001229"
     CDS             11242..11763
                     /gene="rimM"
                     /locus_tag="ACHBB7_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM9209191.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11753..12514
                     /gene="trmD"
                     /locus_tag="ACHBB7_001230"
     CDS             11753..12514
                     /gene="trmD"
                     /locus_tag="ACHBB7_001230"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM9209192.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12650..12997
                     /gene="rplS"
                     /locus_tag="ACHBB7_001231"
     CDS             12650..12997
                     /gene="rplS"
                     /locus_tag="ACHBB7_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM9209193.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13370..14032
                     /locus_tag="ACHBB7_001232"
     CDS             13370..14032
                     /locus_tag="ACHBB7_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209194.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14111..17338
                     /gene="fss3"
                     /locus_tag="ACHBB7_001233"
     CDS             14111..17338
                     /gene="fss3"
                     /locus_tag="ACHBB7_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM9209195.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17452..17766
                     /locus_tag="ACHBB7_001234"
     CDS             17452..17766
                     /locus_tag="ACHBB7_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9209196.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17779..18153
                     /locus_tag="ACHBB7_001235"
     CDS             17779..18153
                     /locus_tag="ACHBB7_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9209197.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18163..18507
                     /locus_tag="ACHBB7_001236"
     CDS             18163..18507
                     /locus_tag="ACHBB7_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM9209198.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18578..19930
                     /locus_tag="ACHBB7_001237"
     CDS             18578..19930
                     /locus_tag="ACHBB7_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9209199.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            19990..21132
                     /locus_tag="ACHBB7_001238"
     CDS             19990..21132
                     /locus_tag="ACHBB7_001238"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFM9209200.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21157..21411
                     /locus_tag="ACHBB7_001239"
     CDS             21157..21411
                     /locus_tag="ACHBB7_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209201.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            21667..22851
                     /gene="mobT"
                     /locus_tag="ACHBB7_001240"
     CDS             21667..22851
                     /gene="mobT"
                     /locus_tag="ACHBB7_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM9209202.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            22848..22985
                     /locus_tag="ACHBB7_001241"
     CDS             22848..22985
                     /locus_tag="ACHBB7_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM9209203.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7644 a         4091 c         4955 g         6319 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
     9421 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
     9481 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
     9541 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
     9601 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
     9661 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
     9721 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
     9781 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
     9841 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
     9901 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
     9961 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    10021 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    10081 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    10141 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    10201 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    10261 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    10321 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    10381 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    10441 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    10501 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    10561 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    10621 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    10681 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    10741 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    10801 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    10861 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    10921 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    10981 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    11041 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    11101 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    11161 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    11221 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    11281 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    11341 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
    11401 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    11461 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    11521 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    11581 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    11641 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    11701 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    11761 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    11821 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    11881 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    11941 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    12001 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    12061 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    12121 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    12181 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    12241 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    12301 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    12361 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    12421 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    12481 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    12541 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    12601 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    12661 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    12721 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    12781 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    12841 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    12901 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca
    12961 cggaaaagct gctcgtatca aagaaatcag aagataaaca cggaaaactt ccttggaaat
    13021 ctgaaatttg tgaaaagtcc ttgtatatca agggcttttt cttttttctc tatcacagtt
    13081 ttaagccatt tttggctgtt ttggaccatt tttcaatcat attcccttaa aaattgtcct
    13141 agattgtccg acattgacta tcagtgtcgt caaaataggg caaaatttag tagtcagctt
    13201 gacgacaaat ataatacgaa aaataccaaa acatttaccg aatttagcaa cttttgaacc
    13261 caagtttctc aaagggtcag ctattagcaa aaaagacaaa acatgtactt ttctactaaa
    13321 aagttgatgt gataggcttc tatctaaata actattagga gtttttatcg tgaagcaaaa
    13381 tgatggacgt atcatttgga aaaatcaaag tgagttaaaa ctgattttaa caattaatga
    13441 attcatcgaa aaacacggaa ttacatctag ccgtcaatac cagaaaaagc tatcagaaaa
    13501 tccaaattct gcaccaagta tgtggttcat taacaaaaaa tatggatcat gggagaattt
    13561 attgattagt ataggacgag aaaatactgg gtatggaaaa tgggctagaa tgtcagaaca
    13621 agaattattg gaaatagtag aagcctttat taagtgcgag aagataacct ctcaacgaat
    13681 gtacgaacaa aaatcagtag ggaagaatat tccttcactt agtaccatta aaaagatgtt
    13741 aggagatata cgtccacttt ttaaagaaaa aaatgatgga tctcgattta ctgatttcga
    13801 actgctgtta gaactaaaaa atgaaataat ccgattaaaa ctacaggatg atttatcaat
    13861 gacaaagttt cgaaaactag ttcaatcccc aaaactacca tcagttgata caattatgaa
    13921 aagaacgaac aaaaattggg aggaattgat ggcagagatt ggatttgatt atcgaagaat
    13981 caaaatttat aagcagagaa acaacctatc caagacaaag aaaactaaat agtatcactc
    14041 taaatcagac gttcaaaatt aaaatttgga cgtctttttt attacccaaa aacgaaagga
    14101 gatttatttt atgaaaaagc caagatttaa gaattggcgt ttgctcgcaa cactgagtct
    14161 attatgccaa acaattggtg gatcgcttgg cccaacgatt gcctttgctg atgaaattac
    14221 tcatccacaa acggtaactg tggaattgga tttggctcac cagtatgctg tcgaaggtac
    14281 atttagcgat ggtcgtccca tgtcagaagt taccgttccg cactatgcag tttacaatgg
    14341 tgtgaagcaa gatattttct gtattgagcc aggggtgccg atttacaatg agttcactcc
    14401 tggttatgag aaaaatccat tgcctgatat gtcggagaaa gcgaaattag tttccgtttt
    14461 atggaaggaa gctggtacgg atgtggatac acatattgtg gcccagaaga tgatttggca
    14521 agaagttaat ggctacacgc ttcactcaat taaacgttct gatggtagtg cagtaaatat
    14581 cgcagccatt gagtcaaaaa tcaatcaagc cattgcggat tatcaaaaga aaccaagctt
    14641 ccataactcc acagtgaaaa ccgtgttagg tcaatcgact actgtgacag atacgaacgg
    14701 cttgaattta tcggagtttg atgaagttgt cgagaatatc gcaaatattg attatcgtgt
    14761 gaacggcaat caactagtga ttactccaaa tgccaattca aaagagagtg gtgaattaac
    14821 cctaaagaag tcagctaata caggaacacc ggtagcttat aaaatggctg gtcaacaaac
    14881 tttgatggcg ggggcaattg ataagcccaa tacctacaca atcaaaattg atgtggaaac
    14941 tgagggttct ttgaagatta aaaaagtcga taaagaaaca ggaaatgttg taccgggaac
    15001 cgttttccat ttagactttg gaaaaacatt acctgcaaaa gacgtgacca ccgataaaga
    15061 aggcattgcg acattggatg agattcctca tggcgcgaaa gtaaccatta ctgaaaaatc
    15121 agtgccagct ccttatacga ttgacactac accaatcacc gcaaccatta aatctggtga
    15181 gactatttct gtgacttcaa aaaatactcg cgaaaaaggt caaatcattt tgaataaaat
    15241 gggggtggaa acaggaactg atctttggaa cgacaaatac tcattagccg gaaatacgtt
    15301 tgccattcgc aaagacagtc caaccggtga aattgtccaa gaaatgacca cggatgaaaa
    15361 aggtcatgcg gaaacaccaa aagagattgc caatgcgttg gaattgggaa cctattatgt
    15421 gaccgaaacc aaagctagta atggctttgt gaataccttt aaaccagtaa aagtcgaatt
    15481 gaactatgtc aatcaaaccg tggctcttgt taccagtaac gtaaaagggc aaaaccaaga
    15541 aatcactggt gaaaccactt tgacaaaaga agacaaagat accggtaatg aaactcaagg
    15601 taaagctgag tttaaaggag ctgaatatac tctctttact gcaaaagatg gtcaagcggt
    15661 caaatggagt gaggctttta aaccagagtt agtgaaggga acgaaagctt ctgatgaaac
    15721 ggtgactttg actttagatg aaaagaatca agttgctgtt aaacacttag cgattaatga
    15781 gtattactgg caagaaacca aagcacctga aggatatacc ttggatgaaa caaagtaccc
    15841 tgtttccatc aaaaaggtgg atgataacga aaaaaatgcc gtgattaccc gagatgttac
    15901 ggcaaaagaa caagtcattc gctttggctt tgatttcttt aaatttgctg gatcagctgc
    15961 tgggactgcc gaaactggct tcaatgatct gacctttaaa gtatcgccat tggaaggaac
    16021 cagtgagatt acaggtgccg aagatgaagc aattacagct tataacgagc aactaggttt
    16081 tgatggctat ggcaagtttg aaaatcttcc ttatggggat tatttacttg aagaagtaga
    16141 ggctccagaa ggatttcaaa aaattacgcc attagaaatc cgttctactt ttaaagaaaa
    16201 taaagcggat tacactaaga gtgaatacat ttttacgatt accgaaaaag gacaaaaaca
    16261 accaattaaa acggtgaccg ttccttacga gaaactgacg aataaagcat tttctgttag
    16321 cttgaatcgt ttgatgctct atgatttacc tgaagaagaa gatagcttaa cttctcttgg
    16381 gacttggaaa gacggaggca aagaattgac agaccttgat tctaccgagc tagttgataa
    16441 attgagctat aacttgcatg aaatcaaaga agactggtat gtggtagcgc aagccattga
    16501 tgtggaagcg acaaaagctg cccaagaaaa agatgaaaaa gccaaaccag tggtaattgc
    16561 cgaaacaacc gcaacgttgg cgaataaaga gaaaacagga acttggaaaa ttttgcataa
    16621 attaaccgca gaacaagttt tggataaaac catcgtctta ttcaactatg tgtatgaaaa
    16681 caagaaaccc tatgaagcag gtgatgatcc agtagcgaaa gatgctagct tgaacaatca
    16741 agcccaaact gtcaattgta cggtggaacg ccatgtttcc atccaaacaa aagcccacct
    16801 agaagatggt tcgcaaacct ttactcatgg ggatgtggtg gatatgttcg atgatgtgtc
    16861 gattacccat gatgtactgg atggctcaaa agaagctttt gaaacaattc tgtatgcatt
    16921 acttccggat aatacgacca aagaaatttg gaaatctggc aaaattgatt atgaagtgaa
    16981 tgacaaagaa ttcactaaaa ccgtacttgc ggaaaaagtg gacacaagta aatatccaga
    17041 aggtacaagt tttaccttca aagaaattaa ctatggcaaa gacggaaaca tcaatgggaa
    17101 acacaatgag gatctaaaag aaaaaactca aactttaaca cctaaaaaag ttccgacaac
    17161 accaagtgca ccagaacaac cggaaatacc aactgttcca agtgactctc aagaatctag
    17221 tcctacagta aagacattcc cacaaacagg ggagaaaaat tctaatgttc tgctgtttat
    17281 tgggtttacc ttgatctttg cgactgctgg gtattatttc tggaatcgcc gaaactaagg
    17341 tgatgtgatg aaacaactaa ataggaaaaa ccaacgtgcg cgaccgcctc ctaaaaaaag
    17401 tggtcgcgct atttttgtat ccaaaactaa aaatgaaaag aggaaaagaa gatggaattg
    17461 aaatttgttg tgccagatat ggccgaaacc tttggaaaga taaggtatgc cggcgaaggc
    17521 gaagttttaa ctgaaggata tgggcggaat actacagtaa ttggtcgtag ttatcatttg
    17581 tattccagta agcaacgagc cgatgatatt gaagtggtgg tagctgcgga agccggcgaa
    17641 aaggattttg accaagatca accactaaaa gccgtgaatc cccatttggt tgccaaaggc
    17701 tatgagattg agaatcgtgg gtttaccgac tacgtgttgt atgtcgatga tttagtgaaa
    17761 gcataggagg aaaagaagat gagattaaca gaaggtattg ttgtagattc aggactaacg
    17821 tttgggaaat tacgattttc cgcattacgc cgagaagtac ggaagcaaaa tgaggatgga
    17881 acggttagta acgaggtaaa agaacggact tataatttga aatcctctgc gcaaggtcga
    17941 atgatccaag ttagtatccc agcaaatgta cccttacgcg agtttgccta tgacgcagag
    18001 gtagagttgg tcaatccaat catggatacg gtcgccaatt atgttttccg agaaggaact
    18061 actgtcaatt ggttcattaa ggccgatgat ttagtcttga aacgacaacc agatcaggga
    18121 aattctacaa accaaaatga aggaaagaaa taggtgaagc gtatggaaat taatgttgaa
    18181 catattttag attgtctcga tcaatatgga aaaggtgagc taacggagga acaactcact
    18241 aaagcattaa cctatgatga aaaaatgttt ctgattatgc atcaagggtt actggaagta
    18301 ggtaacgatg tcgaagaaga ttttgatgta ctgaattggt taggggaaga agagtccttc
    18361 tttatggtta tcgaagtaaa tgaaagtcta tgtcgagaag ccgaaaccgt attggaagaa
    18421 attggtgtgg aaatgccgga tgccattgaa gtttttttaa agcaactagt ggaaacgaaa
    18481 caactcccta tagcagtaaa tgactagcat aactgcttga aattggatca atgaaatcct
    18541 atgaagcttt tttcgattgg aggtggaaac cataaaaatg tacaaagggc atcgtatacg
    18601 ggcaggagat cagcacttgg tttatcactt tgttcttggc tggctactcg ctctgttcat
    18661 cggttggatg agcgtctttt attttcaaga gcttagacaa tttgatattt ctaagctatc
    18721 actttctact atagaaatcg ttcgttctat aaaagattta atctatttgc taggaagtct
    18781 cgttctttca ggaagcacga tgttgcttta tatacacttt tttcaggatc attggcgcag
    18841 tttgtggcat cgacaaaagc tagcccggat gattctagaa aatcattggt acgaagtaaa
    18901 acaaactcag agtgaaggat ttttcaaaga cctcaatagt agtcgaacca aagagacaat
    18961 cagttacttc cccaaaatct attaccgtat gaaggatggt ttactttcta ttcgcgttca
    19021 aatttcactg ggaaaatatc aagatcagct tttaaagctg gaaaagaagt tagaaagtgg
    19081 tctatattgt gaactagtag aaaaggaact caaagactcc tatgtggagt acactttact
    19141 gtatgatata attgccaatc gaattgggat agacgaagta gtggcagaaa gtggggcttt
    19201 gcgattgatg aaaaatcaag tatgggctta tgattcttta cctcatatgt taatcgctgg
    19261 cggcacaggt ggtgggaaga cttacttttt actcactatc atcgaggctt tattaaaatc
    19321 tgatgccgag ctttttgttc ttgatcccaa aaatgcggac ttagccgatt taggtacggt
    19381 aatgcctcat gtctattccc aaaaggagga aatttctgct tgtgtggaag atttctatga
    19441 gcgcatgatg actcgtagca aggcaatgaa agaaatgccc aactacaaaa caggagagaa
    19501 ttatgcgttt cttggacttc caccaaactt tttaatcttt gatgaatacg tggtttatat
    19561 ggaaatgtta acgacgaagg aaagtgcggt gattttgaat aagctcaaac aaatcgtgat
    19621 gttaggtcgt caatctggtt tctttctgat tctagcttgt caaagaccgg atgcgaaata
    19681 tttaggagac gggattcgcg atcaatttaa tttccgggtg gctttgggtc gtatgagtga
    19741 actgggatat tccatgatgt ttggcgaggt tgataaaaaa ttctttatga agcgtatcaa
    19801 aggtcgaggc tatgtagata cgggagggag tgtgattagt gagttttata cgccacttgt
    19861 accaaaagga tatgatttct taagggaaat tagcgacatt ggtggattaa acgttaatac
    19921 ggaaggataa aataatagta tgtagcctag gagtgtcaag aatcctgggc tttttttacg
    19981 aggtgattta tgacattttt agatttattc gctgggattg gtggcttttg cttaggtatg
    20041 gaacaagcgg gtcaccagtg tatcggcttt tgtgaaattg atgattttgc tcgccagagt
    20101 tataaggcaa tccatgatac aagtaaggag gtggaaatgc atgacatcac aagtgtatca
    20161 gacgagttta ttcaatctct cggaccagtg gatatccttt gcggtggatt tccgtgccaa
    20221 gctttttcaa ttgcgggaaa gcggcaagga ttttccgata ctcgaggtac tttattcttt
    20281 gaaatcgctc ggttcgccgc tattctccaa cctaagtttt tattccttga gaacgtccgg
    20341 ggattactca atcacgaagg aggggctacg ttcgagacga tcctccgaac gctggatgga
    20401 ttggggtatg atgtggaatg gcaagtgctt aactcaaagg cctacgttcc ccaaaaccgt
    20461 gagcggatct tccttatcgg acattctcga gacgcatgta ccgaacaagt atttcctatc
    20521 attggatcgt ctccaacatc tgatcaaaac atcagaaact tattaaatat caacccttcc
    20581 aatcgaggaa tggggggaca agtctatggg tcagatgacg tagcgcctac cttgaccggt
    20641 gatgaaggaa tcaaaatcgc attgccggta gatgatggaa tttctgtagc gggtatgtta
    20701 ctgggaaatt ttgaacaagg caatcgggtt tatgagataa ctggaacagc gcctactttg
    20761 tcaacgaaac aaggcggaac gaagatcatg cttcggaaaa acgaaacaga atatcaagag
    20821 gtgaagccag ggaacagtgt gaacctggct tttcctaatt ccacaagtcg tcgaggccgt
    20881 ctgggaaaac aaagtgtgca taccttatta acaggagacc aacaagcagt cgtcacggat
    20941 cagtatcaaa ttcgaaagtt aacacctagg gagtgctggc ggcttcaggg ctttccggac
    21001 tgggcttttg atcgagcttc tcaagtcaat tcagatagtc agttgtataa gcaggctgga
    21061 aattctgtaa cagttccggt catttttgat attgctagaa gattaaagga ggtaaaaaca
    21121 gatgatttat gatgagttga ttggggaatc tactgggtga ttggcaagat tcagtcagat
    21181 ccggaattgg aagaagaact gtaccggctc aattttgata ttcgcaaaaa cggggtgaaa
    21241 gtccctgatg atccgtatgt aattgatgag gaaacggatg ctcgaatgga gctgaatcaa
    21301 gtcatcagtg aattggaacg aattgcggac tttgctaaag aacctgatat ccgccaatac
    21361 ttgtttgaga taaaagcaga gttggaaatt gagggtatta ccgcggagta acggccaaat
    21421 aaaccactga taggcactga ccgcggggga tttgcgaagc agaaatcctc cgtggtgctg
    21481 tgcctgttta tggacggcgt tagccggcca tgatgtctca ccccccgtat ctaacagggg
    21541 ggtacaaaaa cacaaaaaat aagcaagcaa cgataaggaa tggctgtcct atcaaggata
    21601 ataagcaatt tgtgtagatg ttaactgatt tgaaaaagtt aacaccttaa atttggaggt
    21661 gagaacttgg cacaaaggaa tttagattat cggctattaa aagaccggcg taatgaatat
    21721 ggcgtttcac aaaataaatt agctacgact tgtggactta gtcgcccgta cttaaatcag
    21781 atagaaaatg gtggcgtaac cgcatcgaca aaaaccatga ggaaaatttt tgaccaactg
    21841 gaaagtttca atcccgagtt acctttaacg ttactgtttg actatgtaag gattcgcttt
    21901 cccacaacgg atgcgaggaa aatcattcaa gagattctcc atttgaaatt tgattatatg
    21961 ctccacgaag attacgcctt ttactcctat caagaacaat atgttatggg agatattgtg
    22021 gtgatgttgt cacatgaaga agataaaggc gtccttttag aattaaaggg tcgtggttgt
    22081 cggcagtttg aaactttttt actcgcccaa aagcgtagct ggtatgactt tttcgaagat
    22141 tgtctaaaag ccggtggcgt gatgaaacgc ttagacctgg caatcaatga tcgagttggc
    22201 ctgttagata ttcctgattt aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt
    22261 cgtaccttca aaagctatcg ttccggggaa ctgttgaaac ctgatgataa agatggtatg
    22321 ggcaatactt tgtatattgg cagtcttaag agtgaggtct atttctgttt gtacgaaaaa
    22381 gactatgagc aatatatcaa gttagggatt cctttagacc agaccgaaac gaaaaatcgt
    22441 tttgaaatcc gactgaaaaa tgatcgagct tatcatgcaa ttcaagattt gttaaaaggt
    22501 cgcagtattg aaagtaccac attttccatt attaaccggt acttgcgatt tgcggataag
    22561 gtggaaggca aaaggcgaac taactggccc ttgaatgaac aatgggggcg cttcattgga
    22621 cggaatcaga aggaaattca attaacttct gaaccgaagc cttataccat tgaacgaacg
    22681 ttgaactggc ttggacgaca agtcgcccct acgtggaaga tggccaagga gttggatcgg
    22741 ttgaatcaga caacgtacat tcaggatatg gtaaataata ctcgactatc agaccggcac
    22801 aaaaagattt tggagcaaca aagtatggca attgaaaatt tgattatatg aaaagaggaa
    22861 tcgtgatgga aattgtgaaa gatattttat tggtgtttgg tggcagtatg cttggtgtta
    22921 ctgtgatgtg cttgatgcat gcaagtgcag cggcggaccg agcaatggaa aagaaaggaa
    22981 agtgatgaaa tgaatttcgg tcaaaattt
//
LOCUS       DAYYKN010000040        21982 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000040 DAYYKN010000000
VERSION     DAYYKN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.040"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..1166
                     /locus_tag="ACHBB7_001242"
     CDS             201..1166
                     /locus_tag="ACHBB7_001242"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9209204.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1265..2437)
                     /locus_tag="ACHBB7_001243"
     CDS             complement(1265..2437)
                     /locus_tag="ACHBB7_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9209205.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2424..3569)
                     /locus_tag="ACHBB7_001244"
     CDS             complement(2424..3569)
                     /locus_tag="ACHBB7_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9209206.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3583..4507)
                     /locus_tag="ACHBB7_001245"
                     /pseudo
     CDS             complement(3583..4507)
                     /locus_tag="ACHBB7_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4624..5703
                     /locus_tag="ACHBB7_001246"
     CDS             4624..5703
                     /locus_tag="ACHBB7_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9209207.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5700..6328
                     /locus_tag="ACHBB7_001247"
                     /pseudo
     CDS             5700..6328
                     /locus_tag="ACHBB7_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6438..6665)
                     /locus_tag="ACHBB7_001248"
     CDS             complement(6438..6665)
                     /locus_tag="ACHBB7_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9209208.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6768..9356)
                     /locus_tag="ACHBB7_001249"
     CDS             complement(6768..9356)
                     /locus_tag="ACHBB7_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM9209209.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9376..10035)
                     /locus_tag="ACHBB7_001250"
     CDS             complement(9376..10035)
                     /locus_tag="ACHBB7_001250"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9209210.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(10084..10587)
                     /gene="trmL"
                     /locus_tag="ACHBB7_001251"
     CDS             complement(10084..10587)
                     /gene="trmL"
                     /locus_tag="ACHBB7_001251"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM9209211.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10808..11674)
                     /locus_tag="ACHBB7_001252"
     CDS             complement(10808..11674)
                     /locus_tag="ACHBB7_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM9209212.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11783..12424
                     /locus_tag="ACHBB7_001253"
     CDS             11783..12424
                     /locus_tag="ACHBB7_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM9209213.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12486..13847)
                     /gene="mgtE"
                     /locus_tag="ACHBB7_001254"
     CDS             complement(12486..13847)
                     /gene="mgtE"
                     /locus_tag="ACHBB7_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM9209214.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13865..14764)
                     /locus_tag="ACHBB7_001255"
     CDS             complement(13865..14764)
                     /locus_tag="ACHBB7_001255"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9209215.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14766..15563)
                     /locus_tag="ACHBB7_001256"
     CDS             complement(14766..15563)
                     /locus_tag="ACHBB7_001256"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM9209216.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15541..16245)
                     /locus_tag="ACHBB7_001257"
     CDS             complement(15541..16245)
                     /locus_tag="ACHBB7_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM9209217.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16376..16951
                     /locus_tag="ACHBB7_001258"
     CDS             16376..16951
                     /locus_tag="ACHBB7_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM9209218.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            17069..17746
                     /locus_tag="ACHBB7_001259"
     CDS             17069..17746
                     /locus_tag="ACHBB7_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM9209219.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(17825..19633)
                     /gene="pepF"
                     /locus_tag="ACHBB7_001260"
     CDS             complement(17825..19633)
                     /gene="pepF"
                     /locus_tag="ACHBB7_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM9209220.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(19809..20807)
                     /locus_tag="ACHBB7_001261"
     CDS             complement(19809..20807)
                     /locus_tag="ACHBB7_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM9209221.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(21033..21686)
                     /locus_tag="ACHBB7_001262"
     CDS             complement(21033..21686)
                     /locus_tag="ACHBB7_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM9209222.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         6489 a         4698 c         3812 g         6983 t
ORIGIN      
        1 ttttttgtgt ttttcggtct tcggaccttt ttcattttcc tagacttctg ctataataac
       61 caagaattaa ttttgtaaca gatttttttg aaaaaacaag tttctttcat tcaaaaaata
      121 gaaaaagctt gctaattatt ggttaaccag caaacaggag tattaaaata tcttgtttta
      181 ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat ccatcggcag
      241 gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa aataagcata
      301 ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt gctgaaactt
      361 tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc gatactttcc
      421 cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat actttctatc
      481 agatggaagt agaattccca gttgcttata ttccagcagg ttctggagat gattttgctc
      541 gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta gcagctcaat
      601 caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag
      661 ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca aatcattctt
      721 ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta ttctctattc
      781 tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga ggaaaacaat
      841 taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt ggcggaggaa
      901 tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac
      961 gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa aaacaattga
     1021 aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca acaacaccgc
     1081 aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc acaataacca
     1141 caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta catctggaac
     1201 accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt tttttactat
     1261 gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta cccaaaataa
     1321 attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg aaccaaaaag
     1381 attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta ttttgaacaa
     1441 accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag tattctgctg
     1501 gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg atcccaccgc
     1561 tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg atcggcaata
     1621 aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag gtgtggatca
     1681 ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa ctaatcaaac
     1741 taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg tttcgtttac
     1801 tgatctcttt gcgattaaat gctagattga tgacgctgaa accgctaaaa atggatagaa
     1861 acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc atccgctgac
     1921 tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt gtctgttcca
     1981 tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt
     2041 tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt atcttgatcg
     2101 tcggcatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt aagataatgt
     2161 ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca tccaatgtaa
     2221 cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc gactcttctt
     2281 cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca atattttgcg
     2341 caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc gatttatttg
     2401 ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct ttccatccaa
     2461 gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt ggtataaaaa
     2521 ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa gttgacgaga
     2581 tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg ttccgacccc
     2641 atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct atccttttgc
     2701 accagattca tttttggtat caaattacca accaatgtac ctaacaaaat cgcacacaac
     2761 gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc tacttgatag
     2821 ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt aaagcttgct
     2881 gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa gcgaatacca
     2941 tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat cgtcccgtag
     3001 agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat attacgacct
     3061 gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg cgcgagatca
     3121 acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa aaattgatcc
     3181 atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag cgaaacagat
     3241 gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc attcgcttct
     3301 acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac ttgttctaag
     3361 actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat tccagtatta
     3421 atcgtttcga gattgccacc ttgatcaatc tcgccaaagg ccgccataaa catcaatcct
     3481 aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag cagtattttt
     3541 gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc tcaaatcctt
     3601 gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta tttttccgta
     3661 actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat ttagttcagt
     3721 agttgttact tggagctgac cttttttata agcgattcca tcagaagcta ttgttcgctg
     3781 acaacacgtt ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca
     3841 tatcttttag agattctttg gttcctttag caatcacctg tccttgatcc atgatcacga
     3901 tctggtcgca aagaatttct acttcttcca tgtaatgtgt tgtatagaca atggttgctc
     3961 cttgtcgatt cagctccttg atactttcta aaattttatt ccgactttgg gggtctacag
     4021 ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca catgcgatgt
     4081 tcaaccgacg tttcaagcca ccactaagtt ctcctggacg aaacgaaaca tatttttcta
     4141 atcccactaa ttgaatcacc tcttcaatca aggctttcct tttctctttt tcggaaacat
     4201 ataaaccgca aaaataatcg atattttctt gcacacttag ttcgtcaaag accgcaacgt
     4261 cttgaggaac gaccccaatc attgccttga cagcataatt ttcaggagtc attgttgtcc
     4321 cgaacaattt aatggttcct ttgtcataat ttaacaaaga aagcatacag ttgatggcag
     4381 tcgatttccc actgccgttt ggtcctaaca gacctaagac ttctccttct tgtacttgca
     4441 gatcaaaatg attcaatgct acaaaatctc catagcgttt gactaattta tcaatttcga
     4501 taatcatcat gaaaaactcc ttccaaattt cttgttgatc tgactataat aaagtgcaag
     4561 aaaagaaact agtgacgatt ggctaaacca cacatgacat ttgtcacaat gaaggagtct
     4621 gctatgcata aatctcggct actcaacgaa actagctttc tactggttat ctcattgctt
     4681 ttgctggtaa gtcaaggatt cgttgctatg caagttatct atttattgat cgccgtttta
     4741 ttcactggtg tcagttattt actgcctaca aagccaaatt ggattttcca agttattcta
     4801 tttcttttat gtgccttttt acctgatctt gtcctgtttc tgccagcgac gatgcgagtg
     4861 atcgcccctg aagaaagcca ccttgattac ttgtttttgt taggtttttt gttgatttcg
     4921 acggttggta atcatccccc catcaaggtc gcctttgtcg taattttgct gtttttttcc
     4981 ttttacttac gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta cattgtctcc
     5041 aaagatactc attgggaaaa acaacaagag ttatcggaac aaaataaagc attggccctg
     5101 tcacaagaaa cacaattagc cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac
     5161 atccatgaca atgtcggtca tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa
     5221 atgatcaatc aagaaccaaa actaaaagaa ccattagaaa atttaagtgc caccgttcac
     5281 actgggatgg atcggattcg tcaaagtgtc catgacctcc atgagacatc gatctctttt
     5341 caacactcct tagagtttct gatcgctgat tttccttctt ctgttacgat tgaaggaaac
     5401 ttgtttgaag gattgaatga aacgcagcaa aactgcttct taatggtcat caaagaagcc
     5461 ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg
     5521 ccggcctttt atcgcttgca aatcacgaat gatggcacca gcccacaaaa aaattctgct
     5581 cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat
     5641 attttccaag aaaaccagca atttcagtta acgatcattt taccaaggga gggacaccaa
     5701 tgataacagt agtcattatt gagatgatcc atttgtgacg aaatcgttac aaaccatttt
     5761 agaaagcacg aaagagatcc gtgtgttagg cattggtcat tgtgccaagg atgcgctagt
     5821 cttatacgaa acccatcgtc ccgatgtttt gctgacggac atccgcatgc ctaaacaaac
     5881 gggcatcgat gctgccaaag aaattttggc taaatttccc aaagccatcc ttttgctttt
     5941 gacgacattc aaagatgaag aatacatccg cgaagcattt tccatcggtg tcaaagggta
     6001 tttaatcaaa cagaacttgc aagcgattat tccttctgtc aaatccgctt ataatggaca
     6061 ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga ataaaccgac
     6121 gattgatagt catgcttatg cttcttttag tgaacgtgag cttgcaatca tcaaagaagt
     6181 agctgccggg aaaaacaata aagaaattgc agatgccttg tacttgagtg acggcactgt
     6241 tcgcaattac atcagccaat tactagaaaa gctagattta cgggatcgca cgcaattggc
     6301 tatctatttt tatcagcatt tgcaataagt cttatctaac acaaaaaaac ctgaatgatg
     6361 ctccttcgca tcattcaggt ttttttcaca tcttctattc aattgtcccg caaaccattt
     6421 cctcacgatt gaaagaatca aggattcgca gggctgccag gattcgttgg attgttcggc
     6481 gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt ctagttttga ttcttcacga
     6541 tcgtctcgtt ctacatcagg actttcattc aatttgaatt ctttttccat ctcatctaac
     6601 tttttaaagt cattgccaga ttcttttgct aatttcgcta atttatcttc ggtttcttta
     6661 gccataaagt aacgcccccc ttttgatttc agcttagcat atcacactga aaaaacaaaa
     6721 gaaaacgcta accaacgatc atttttagct atttctcatc tttaaaatca agcatttttt
     6781 gccggtataa aatcgtaggg agaactgttt tccataccaa tcatcggatc tactaactgg
     6841 ttatcgatca cttctgctgt aagaacgaaa tctttagaaa cagcaggttc tacctcttct
     6901 ttcttttctt ctccaacaaa tgaagaaaac agatctggtt ccactgtttc cattacttgg
     6961 tttttcgttt cattcgtccc actgtcatct tctacaggta gcttttcttt tacaacccgt
     7021 ggctcttttg tatagttccc atcttcaaat ggatcatttc cgaccaaaga atcttcataa
     7081 gctgctagtt tggtccggag agtgatattc attttatcat tatccatgat ccttgatttc
     7141 aacgtagtca tccggcttgc tgattcattc gctacacaag tgacataggc ttgctcttcc
     7201 cctaacaaaa tcaacagttc cttactgcga gtcactgccg tgtagagcaa attacgctga
     7261 agcatccgct gatattgatg gaccatgggc aaaataacca tcttgaattc actgccttgt
     7321 gatttatgga ttgagcaaca ataagaaaga gtgattttat tccattcgtt tcttttataa
     7381 gtgacctcat ttgcatcaaa ctggataact aattcatcca ctttatcttc agaatctttt
     7441 gccaagatga tacccgtgat ttctcccata tctccattga atacattcaa ttcgggagta
     7501 ttgactaatt ggaggacttt gtctcctatc cgataaacgg tgtcgttcca atgtacttcc
     7561 ttttttccag gtttccctgg attgaatatc tcctgcatca ttttatttaa cgcatcgatc
     7621 cctgcaggtc cccgatacat tggtgctaat acttggatat cttgtgcacg gaatccctta
     7681 tttttcgctt tttccacaat tttagagatc aagggttcaa tttgataagc atcacttcgg
     7741 aaataggaac gatctttttg attctttgtg aaatcttttg gcagacgtcc ttctttgatc
     7801 tcatgggcaa gaggaataat actagaacca tcaccttgcc gataaatctg attgagttcc
     7861 attttgggaa tttgttcgat ttccaaaaga tcatggagta cttgaccagg accgacagag
     7921 ggaagctgat ctttgtctcc tactaagata acttgcatat tcgtcggaat cgctttgaat
     7981 aatgtgttag ccagccatgt atcgaccata gacatctcat caacaatcaa aagtccgcct
     8041 tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc cagaagacga
     8101 tggatcgtac tggcaggcag acctgtcgtt tcgttcattc gtttagctgc tcggccagtt
     8161 ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt ttgaaggatc caattccagt
     8221 ccgttcaact ctgcaaaaag attgacgatt ccgttgatca cagtagtttt ccccgtacct
     8281 ggtcctcctg ttaaaataaa tagaggggaa cggattgctt cagcgatagc ttgttcttgc
     8341 gaaggtccat actgtatgcc gaatattttt tcgatacgcc gaatattctt ctgcacttct
     8401 ttttcaggat aatggatttc ttttttacga ctgagaaggc gctggatcga attaccgatc
     8461 ccccattcag aaaagtacaa actgttttcg taaagattag tctcttcttg ttgtatcttt
     8521 ccttcttcaa caagttggat aattacagta gcaacctgat caggctggat ttcaattggg
     8581 cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta
     8641 tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc
     8701 ggtgaatctg ctgcaatacc gatttgttcg gctagattat ctgcacgttt gaacccgatt
     8761 ccttctatgt cttcgactag ctggtacgga ttttcttcga tgatatccaa agactcgttc
     8821 ttataggcct ggtagatact gaaagctaac tgactaccga acccgtaacg attcagtccc
     8881 atgatcactt gatccatccc gtggtttagc cgaatcgtct cagcaagcat ttctcttttt
     8941 ggcttcgtca aaccggagat ttgttttaat accgaaggat cagcaatgat tttttcgata
     9001 gcatcttctc ccagcaattc tacaattttt tctgctgttt tcttcccgat tcctgggaat
     9061 ttttcgctgg aaagatacgc tacgatccca tttgcagttg ttggcttttc ttgctcatat
     9121 ctttcgagtt taagctgcct tccgtattta ggatgatcca ctaattctcc ataaaaacga
     9181 taagtctcat tttcttgaat atcgccgaaa cttccggtga ctacaatctc tttttcacgg
     9241 taatcagctg tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt actaggattt
     9301 tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca tttcttctgg ctgcatcgga
     9361 agccgcctcc ttcttctata gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt
     9421 ttggttccat actttcagtt catatggcaa taatctgcaa ggctcttcag tctctaaaat
     9481 cgtcaaacta ctattgaaca atccacccat ttcacgcaac tcacttagct cttttcctgt
     9541 catccattga atggctgcag ttaaagaagc tccatgtcct acgaataaaa gtgggccatc
     9601 tttatgttgg gcaaccgcat ctgctacaac agtttcgata cgtgtgattg ccgattcaat
     9661 cgtttctccg tgaaaaggag aaggatcata cagatctaaa tgattacgca aattcggcag
     9721 attatccgga tatttctggt acatttcatc gatcgactga ccttctaaag tacccagccc
     9781 tagttctctt agcttatctg tataaaaaat ctctaccgga cgagcaagct ctttgttgat
     9841 accttctgct gtatcacgtg ctcgctttgc tgtactggaa taaattgctt taaaaggaac
     9901 atattttacc tttttcccaa atgctttgat ttcttcgtaa ctttgtggca aaagcggaga
     9961 atcgccattg ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc catgtcgggt
    10021 aaaatatagt tccatacgca gcaaactcct gtctttcttt tattttactc tataataaac
    10081 tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat tctaaattag ggaatccttg
    10141 ctggcgaagt gcttcataca ctatcattgc agctgtattc gataaattca atgagcggac
    10201 atgctcatca ttcataggga tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc
    10261 tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc tcttctccat cagcaaaatt
    10321 ttcgtcacta tatactttat gggcaaattt tgagatcaaa tgtagtttac gattacctaa
    10381 aaatgttaaa aaagcttcta aattactgtg gtaagtaata tttacatcat tccaataatc
    10441 caaccctgcc cgttttagat gtttgtcatc agttgaaaaa cctagtggct cgattaaatg
    10501 aagcggagta ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg cagggattaa
    10561 tggttcaaat aaaacgatat gattcatcag gatcattcct ctcatattta tgttcgtttc
    10621 tattttctga caatttgatt tttgcataaa aaaaaacgta tcaactcggt gtcagtcact
    10681 gcgagtcgtt acgttagtga agctcttctc actaactttt atcagaacag gaacctgata
    10741 aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc tcaacttttt tttgcaacct
    10801 ctactcatta aaaactgcta gaaaaacgtt ttctcttagt tccttttaat aattctatgt
    10861 caattgtaat cttcttcaaa ggattcttat ataagtctgc tttgattcgc tcttctacct
    10921 gccattctaa aggtgacatg gccaaaagat ctttttgatt ttcctctggt atctcaaatt
    10981 cataggttac acgctgacgt tcagttaatt tcattgcttc agcaaacttg gctactacac
    11041 gttcattgga ataactctgt ttttcaggtt gattcggata aaaagcctga cgtagttcct
    11101 ttaaataatt ttcttgtggc accaccttca acacttctcc atcttctgag agtacacgtt
    11161 ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag
    11221 caaacggcaa attggtcaaa tcagctacac accaaaatgc ttcggcagat tgttctgtag
    11281 ctgcgtagac tccttctttt gaaatatcaa atccaacagc attttgaatc tttttcaatt
    11341 ttgaaagttc attcaagaag ctaccttctc cgcagccaac atctaatagc gagacttctt
    11401 caggtaaatg ctgcgcaata gtctcaataa cgggctgata catgccagat tggatcattc
    11461 tacgccttgg aacgaacatt ccttgatcgt attctgtttt aaccgcatgg gataaaaaat
    11521 aaagtgttcc ttttttggaa aggtcaaaac ggtgattatt tttgcatatc aagccattat
    11581 cagacttttt tacttcttct tggcaaaccg gacagcggaa cagctgatga ttttctgcca
    11641 aaaactggcg tcctcggtca atttttttta acatagttcc ccctcatttc tgttttatct
    11701 taccgttaga tgctcacttt ggcaactgct aaaaatatga tacaatgcct agaagaaagt
    11761 gaaagataaa ggagaagatt agatgatcaa agaattttgc gctgaaaact acacatctat
    11821 tcctctagca attgctagag gagccgatcg gatcgaattg tgtgacaact tagcggtcgg
    11881 tggcacaacc ccgagtactg gtgtgatcga agaaacgtta agttatgctg gggaaaaaga
    11941 tatccccgta atgacgatta ttcgtcctag agggggcgac ttcgtctata acgatatcga
    12001 attgaaaatc atgcatactg atttgattga agcaaagaaa ttagggacgg atggcgtagt
    12061 atttggttgt cttactcctt caggctggct agacgaagag gccttagaac tattgatcga
    12121 aacagcagaa gggttgcaga tcaccttcca tatggctttt gacagtattc ctgaagatcg
    12181 gcaattcgaa gcaatcgatt ggctggtaga acatggtgtt gaccgtatct tgactcatgg
    12241 cggtcctgct gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat tgattgccta
    12301 tacagatggc cgcttaatca ttttgcctgg tggtggtgtc actgcagaga atgcagcaca
    12361 tgtagctaaa gaattgaacg taagcgaagt acacggaacc aaaatcgtgg atcttcagtt
    12421 ataatcttat aaacaaaaaa acatttgcaa caggcataat gcttattgca aatgtttttg
    12481 tttttttaca taaaatagcc cataaataaa gaagcaatat tgaaataaat cagtacactc
    12541 gtcaaatcgc tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc aaatcctaga
    12601 cgatccatta gcatcgggat taaactacct gcaagattcg ccactgtgat tgcaaatagc
    12661 atagccatcc ctatgacaaa ccccaagata aaattatgtt tccaaaaacc tacgattaga
    12721 aaaatcgtga tccccgtaac agctcctgtg acaacgccgg tcaagacttc agccaaaaca
    12781 agtcgaacaa atccatgatc tttctcatca gaaatcgcaa gtcgacgaac agccacagct
    12841 agcgactggg ttccagcatt accggcagtt cctgtaatca gcgaaatgaa gactgctaaa
    12901 atactcgctt cacttaccaa gtcttcatag tgactgatta aagaagcggt cgacatacct
    12961 aaaaacaaca gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac tggattttca
    13021 ttaatttctt ccacattgac cccggcaagt cctgaataat cgctagcagc ttcatcatca
    13081 ataacgtcga tgatatcatc gactgtgacg attcctaaca aatgatcatc ataatcggta
    13141 acaggaagag ctaagaagtc ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg
    13201 cctacatgaa ctgaaagcac tcgttcgctc atcagatcag aaatcattgc gtcgtcatca
    13261 ttcacgatta ggtcgcgcag agaaataacc cctactagct gaccttcttg attaacgaca
    13321 tagatgtaat agattgtttc agctacatct gcttcgttct tcagcacgta catcgcagag
    13381 cgaacagttt gattggctac gatcgaaaca aattcagttg tcatgatcgc gccagccgtt
    13441 tcatcctcgt aatggagaag ttccttgatt tcactggcat cgtctgccga catcaaactt
    13501 aaatatttag caatttgctt tttatccagc gtattaagca agtcaacagc attatctgta
    13561 tacatctcag aaagcatatc agctgcatag ctaggacgca tttcatctag gtattctttc
    13621 atatgttcgt tgtcttcttc aatcacatcg aacatatccg ctaattcttt aggcgacaga
    13681 taggaataga tcagctgcct gttctcttct gtaagagatt ggtagaattg cccctgctca
    13741 tagatatgca tctctaaaaa actgtttctg aatgcctgaa tattattttc ccttaaatct
    13801 tgagtcaact gggcaaatct ttcctctaat tcttgatttt catccaaacc aatctcacct
    13861 cgccttattc ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa ggctgcttga
    13921 actccagcaa ctcttctgta aatggatggt aaaaacgcag atcataacaa tgaagggctt
    13981 gtctttttat tcccagatcc attcgcccgc catacatatc atcaccgagc agggcacagc
    14041 caattgcgga aaaatgaact cttatttgat gtgtccttcc tgtatgcagc tggatatcaa
    14101 ctaacgcgat ttctgttagt cgcttctcta tccagtactc agtctgtgcc gaacgtcctt
    14161 catcagagac cattcgcttc aatagcgaag aataatctcg ggcgatcggc aaatcgatat
    14221 gtccatgtgc cgataaatct gaaagccttc cactgactaa ggcctgatat tttttcacaa
    14281 acttcttttc acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa
    14341 gcatcaaacc agatgtatcc cgatctagtc gcgtcaccac atggattact tggttttcgt
    14401 aaccttgttt tttataataa gcctttacac gattagccat tgtcccattt ggatggtatt
    14461 gggcaggaat agacgaaacg cctgctggtt tatttactac cagaacgtgt tcatcttcat
    14521 acacgatatc gatcggtgtt tcatccaaaa gtacggtttc atgttctcct tcagcaggaa
    14581 tcacaatggt taccttatca tccttagcta gagaaaacaa gacattttct acttgatcat
    14641 taactttgat ttgaccgcct tgaaatttta ttttcgctaa cagacccttt gagatccctt
    14701 tttcttttaa aaaatatttt acttgctgag aatgatcttt ctcataatcc catgtaaact
    14761 gcatatcaat cctcactaat aaaggcatct ttcacacgat gccaaaaatg catatgtcga
    14821 taagaagcaa aatggatccg ttcttctgct attcgataat aaattgagcg gatatttgct
    14881 tttgcgacat tcaattgatc aaccgttaca agataatctt ctgtattttc caattttatt
    14941 tctagccatt catcatgggc aatcacgatc ggagaaccca aagtacgaaa cactcgatta
    15001 ttcagtgaag caatctctgc caactgaaag gcattgatac taggatggat cacagcaccg
    15061 ccgacacttt tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct
    15121 ctaaaacttt caaacagctc gtttttgatg tatacatcgg ctaccatcgt acgattggcc
    15181 cgtttaatcg ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc atccaaataa
    15241 ctgatccgaa catccagcaa gggataacta acactttgtt cccggttcgt tcgtaagctg
    15301 tctactagtt cttccagctc gtaatcccgc caatccgtgt aaaaccctaa atgacctgta
    15361 tgcacgccca agaaacgcac atcattcaga caatgactgt agtgatggaa cgccgacaat
    15421 aatgtgccgt ctccgccgac tgagatgacc aattctggct catgatcatc tcgttgaatc
    15481 cctgcttgtt ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt tgcttcttta
    15541 ttatgaacga tcgctacttt catcccctgc ctcctctctt tcgctttctt tcttcaaaag
    15601 cgttcgatag tagacatcat ctgattttcc tcttccatgg gagaataacc gctgtgcttc
    15661 ttggatttct tcacggatac tggacatctc ttcatccaac tgataggcag cttctgccgc
    15721 acgctgaagt cgttcactca tttcttctgg aaaaacgcct tgatatttat aattcagcga
    15781 atgctcaatc gttgcccaga aattcatcgc aagagtccga atctggattt ccgccaaaat
    15841 tttcttctca ccgctgatca gctgtacagg atactcgatc accagatgat aagaacggta
    15901 gccacttgct tttttatttg tgatgtaatc tcgttcttgg agaactttca tgtcttttct
    15961 tttacggatg atctcaacaa cttgatggat atcttctaca aactgacaca tgatccttaa
    16021 cccggcaata tcagacattt cttcttctaa acgtcccatt ggaatatgcc gtaattgtga
    16081 ttttgttaca atactatcga ctggcttgac tctcccagtg acaaattcaa tcgggacatg
    16141 tcgattttgt tcacggaact gtttgcggat cccccgcagt ttcactttca attctgatac
    16201 agtttgttca tatggtgcta aaaatgcttc ccaatctcgt tccatagctg atacctctac
    16261 ttcattaaca ttcattgttt attctaccat atatgcaaag ttttttcgta ctcttgattt
    16321 atgcattgac caaaaaaaga aaattaggca aaatagacaa aaaggagtcg atcaaatgac
    16381 acaaatgctt gaaatcgaat ataaatctat gctgacaaag caagagtacg agatattcat
    16441 cgctcactat caattaacag ataaagattt ccgtgtacag actaatattt atttcgatac
    16501 cgttgatgaa cagctgaaga aacaaaattg tggcctacgg atccgtttcg ttggtcatca
    16561 agctgaatat acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa cggatacctt
    16621 tataacagaa gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt
    16681 ttttcaaaaa ctttctgatt tgaatatcga tcctgcttca ttaatccaaa ccggaaagct
    16741 tacaacaaaa cgggctgaat tccctattga agaaggactt ttagctattg atgaaagctg
    16801 gggagacaat ctacacgatt ttgaactgga actagaggta ggggatgcat ctgtgggaaa
    16861 aattgctttt ataaattttt taaaacgttt ttctttacct tatcgtcctg ctaagaacaa
    16921 gatccaacgt atgcttgaag ctaaaaatta gaaacctttc attttcacaa ggcacttgag
    16981 ttcatttttt tacttttttc tgttaagtta taggatgtga aagaatctag tcactagttg
    17041 tatagaatct attttggggg agattcatat gatcgaaatc tatttatttg ttaatccttt
    17101 aggggaatac tgtttcgaaa tggaacaaca gttattacaa tttatcgaag aagagtacgg
    17161 gacaccatca aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat
    17221 tggggatgtc atgcaacaaa aaggaatttc tcagaacaat cttgaggaac gtaatcactt
    17281 attctcaatg acttattctg cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa
    17341 aaaaggccgc cgttttttga tcaaattgca agaagcagtc ggctgtaatg acataccata
    17401 ttctcaagaa cttgctgaat ccatatttga agaaatcggc ggcgatttga atatgtttaa
    17461 agaagatcgt cagtctgact tcgttaaaga agtattcttt tctgatcaaa aaattgctcg
    17521 ggaaatgggc atcacaaaac acccttctgc agtcgtttac aattatgcct gcgaccgtga
    17581 ttttggtgtt ttagtagaag taaaaggtac aagtagcctt tggagaatca cagagctgtg
    17641 taaaacaaca gataactcat atcaagtttt taatggtgaa catttacata caaatgattc
    17701 tcagttccat tcaagaacta gccatttaca cttgctttct aattaaaaaa atacgtcgac
    17761 tcccttacaa gagagccgac gtattttttt cgtttctatt cgagaaaatc aaaaatagaa
    17821 agccttataa acgatcgatc aattcttcta attcatttag acgttgttca aacatcgaca
    17881 ttgcatcttc aatatatgca gcctgtgtca tatcaactcc tgcttttttc attacttcca
    17941 caggataatc gctgttacct gcttttaggt aagccaagta gttctctagt gcctctggtt
    18001 cctggtttag tattttcttc gccagtgctg aagcagcaga aaagccagtc gagtattgga
    18061 aaacatagta attataataa aaatgcggta tccgtgacca ttcgaattta atttctgggt
    18121 cttcttcgac tgctggacca taatattttg cattcaattt accataacta tcacttaggt
    18181 attcactagt caatggcaca cctttttcat cttccgtatg catgaaatgc tcgaattcag
    18241 cgaattgtgt ctggcggaaa actgttcctt taaacccatc caagtaatgg ttgagtacat
    18301 aagcccgtac acgagggtct ttttctgttt ccaataaata ctccgttagg atattttcat
    18361 tcgttgtcga agcaatttct gctaaaaaga tggagtagtc gccgtacaca taaggctggt
    18421 tcgaacgagt gaaataacta tgaacactat gtcccatttc gtggacaagc gtaaatagct
    18481 gatccagcgt atcatgccaa ttcaataaaa tatatggatt tgtgtcatag cttcccgaag
    18541 aataagcatc gcttcgtttc cctttatttt cgacaacatc gatccaacgt tcagagaatg
    18601 ctttttctac gatggccatg tattcttcac ccattggttt cagtgcttct aaagcttttt
    18661 cttttgcttc ttcgtacgta aaggtaattg gagcttcccc taagaccggt gtataaagat
    18721 catacatgtg cagtttttcc acttctaata aacgtttccg taattccatg tatcgatgta
    18781 acaaaggcaa atgcttgttt accacgtcta ccaaagtatc gtatacactt tcaggaatat
    18841 gattattgct caaagacgct tctctggcag agctgtaatt acggactttc gctttaaaat
    18901 tatgtccttt tatatgtgtg cctaaagtag aagcaaatgt atttctaaat tgttcgtaaa
    18961 cactgtacaa tcccttaaat gctgcttcgc gcacccttcg atccgtgctt tctagcaact
    19021 gaccatacac gccatgagat aattggacta tttcaccatt ttctccttca atcgttggaa
    19081 aaactagatc tgcattattc aaaacagcaa atgtatcact tgaagcatca aagatttcac
    19141 ctgctccagc aagaagagat tcctgctcag cagataagac atgggctcga ttatctacta
    19201 tttgctggat ataatggcga tagacttcca attttggttc ttctttgaaa tattgccaaa
    19261 tctggtcatc tgacaattgc aatatttctg gttcaaacca ggaaacggct tcactaactt
    19321 tcgaaaacag actgcttgct cttgcataaa gcgcttggta atctgtattt ccagtgtctt
    19381 gatcgttttt aaggtgcgca tatacataaa tgacttcagt ttggcgataa accctcaata
    19441 cgaattcaat cgcatttaaa aattgagaag cgccttgatc aagtgttcct ttgtgttttt
    19501 cagattgttt taactcttct gataattcca aatatttctc atcaaactct tggtcgcttg
    19561 aaaagatctt ggtcaagtcc caagttaaat tttcaggcaa ttcttctcgt tttggcaact
    19621 gctttacttc catgaaatcc actccttttc ttcccttagc tctatctatc aaaatagatc
    19681 atagtctaac atccacatgt cacagcatct aacttttcta ctcattcatg tgtctgcttc
    19741 catcaatttt agcataacta aatcttgttt tacatagaaa accgattcct agcctatttt
    19801 tatacttact attttttcaa agaagacaag tgtatgcatt cgttaaaaaa ctgctgatag
    19861 accatctctt catcaatcat aggaaaaagc cattcccttt tatgttcttt tatttggcaa
    19921 agccatgagt tgaaatcagg aactttctgg ttttccaagg cttcttcgta caacattcga
    19981 taaagaaaaa cctgttcttg taaataaaag aaaaaccgac ttttttgata gatccacgat
    20041 gatagatgca aaagatgttt atgacgcaag tagcattgtt cttgtatgga tactgtattc
    20101 ttttgcttgt gaataagttg gcgtgtcagc catttcttcc catcttcact cactttttct
    20161 atttttttca tttggttcat tttaatttcg ttactagtga atatttcttt aaggtcccta
    20221 ctagaaaaag tccagcagct actgcaaaag gaaatatgcc ctgatacagt ctctgtaata
    20281 tgatgataca aataaagctt ttgtttcttg agatcagctt tccagcaatg cactcctctt
    20341 tttctattat agtagcagca acttttttct atcaatgaga attgcgattg tccaagaaaa
    20401 gaattaccaa gtatccacca tacttggtaa ctgttattca aataattttg ggttctttca
    20461 ataaatctct ggtgagacag tctagaacat tggatttcta ttgctagatt cgccaacaaa
    20521 atatccggcc tttgccgcaa cctcggcaaa taggcttcca aaaatactgg ttctactgac
    20581 tgtcccagcc aatccatgaa gcattccttt aactgtaagt gctcttttga ttctctttca
    20641 ctaaagaccg aacagttatc tgatttttga tgggcaaaat gagcacattt tttctttccc
    20701 tttttcaaaa tcactctttt tcgacagcca cgacaataat atttctttcc tttttctgca
    20761 tgtttagcaa aacagcactt attatattca ttcattgcaa gcagcatttt tctccctctg
    20821 aacttattta cgtctaagaa tttatttaca cgaaaaaaga gggcgtgaaa aagctattct
    20881 gcatcaagta ataagaacag ctaaacaaaa atagttccca atatttttcg tttggttggg
    20941 cttgttaccg cagatgcaag cttttgaaca catttatacg aaaaaaagac tgcgacacaa
    21001 tttttgtcac agtctcttac tataatgctg atttatacaa aataaaattt cgttcgctcg
    21061 atagcattac gcgccatcag acattgtcca tattcattca atgtttctgg tgtacgatcg
    21121 ctcttttcag caaactccaa aattcgagca atctgattct ctacatccgc ttggttgaga
    21181 ttttccatcc agaagtgaac gactaggtaa tagcgctctt cgaataaata caaatcagtc
    21241 caagcagact ctagttcgac atctgttgca agttggatca cttgttcaaa atcttcaaaa
    21301 gcaaaagttg cttgtctttc ctgttcttct agggcgcttc cagataattc ttcctccgga
    21361 tcgctgccaa taatctgctg acgaagaaag tcactcacat cttctgaatc aacagtagaa
    21421 tcttctgatt gagaaatttc ttcatccgga agatttttac tgataaacag ttctaatccg
    21481 tctccttttg gcaaaacttg gaaagtgaca gcttcgcttc ctcggaactc ctcttcgata
    21541 tcaacttctt ccaaaatact ataaaagaaa ttttcgattt ctttgtgatt gccaagtaaa
    21601 tccaaaaatg aaacgccccg ttcctctaaa tcttcatgac caattagtac acggatcgta
    21661 ttttcattaa tatgttccat ttccattttg ctacacctca ctttgccaca atgtttaata
    21721 tagattcatt gtagcgaaat cagcttaaat gtaaagaaaa aaaatctcgc tgttaagcga
    21781 atttaataaa accagcggat ttttcaacct ttcaagctat cttatttaac tataacattt
    21841 ttggcagacg aatactatat agccgacttt tcagctaatt ttccctctct atttttcgtg
    21901 caatcatcat cttctactac ctttttatac atctaatcaa gaatactttt tgtaaaaaca
    21961 aaaaaacttg gaaaaactat ct
//
LOCUS       DAYYKN010000041        21816 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000041 DAYYKN010000000
VERSION     DAYYKN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.041"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..1458
                     /locus_tag="ACHBB7_001263"
     CDS             118..1458
                     /locus_tag="ACHBB7_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9209223.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1676..2119
                     /locus_tag="ACHBB7_001264"
     CDS             1676..2119
                     /locus_tag="ACHBB7_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM9209224.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2623..4041)
                     /gene="nhaC"
                     /locus_tag="ACHBB7_001265"
     CDS             complement(2623..4041)
                     /gene="nhaC"
                     /locus_tag="ACHBB7_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM9209225.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4444..5667)
                     /locus_tag="ACHBB7_001266"
     CDS             complement(4444..5667)
                     /locus_tag="ACHBB7_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9209226.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5698..6537)
                     /locus_tag="ACHBB7_001267"
     CDS             complement(5698..6537)
                     /locus_tag="ACHBB7_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9209227.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6554..7234)
                     /locus_tag="ACHBB7_001268"
     CDS             complement(6554..7234)
                     /locus_tag="ACHBB7_001268"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM9209228.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7236..8309)
                     /locus_tag="ACHBB7_001269"
     CDS             complement(7236..8309)
                     /locus_tag="ACHBB7_001269"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM9209229.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8365..8603)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8646..8879)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8956..9627)
                     /locus_tag="ACHBB7_001270"
     CDS             complement(8956..9627)
                     /locus_tag="ACHBB7_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9209230.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9714..12362)
                     /gene="mgtA"
                     /locus_tag="ACHBB7_001271"
     CDS             complement(9714..12362)
                     /gene="mgtA"
                     /locus_tag="ACHBB7_001271"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM9209231.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12704..12878)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            13051..13488
                     /gene="ndk"
                     /locus_tag="ACHBB7_001272"
     CDS             13051..13488
                     /gene="ndk"
                     /locus_tag="ACHBB7_001272"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM9209232.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13510..14376)
                     /locus_tag="ACHBB7_001273"
     CDS             complement(13510..14376)
                     /locus_tag="ACHBB7_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM9209233.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14536..16629)
                     /locus_tag="ACHBB7_001274"
     CDS             complement(14536..16629)
                     /locus_tag="ACHBB7_001274"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9209234.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17378..18001)
                     /locus_tag="ACHBB7_001275"
     CDS             complement(17378..18001)
                     /locus_tag="ACHBB7_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209235.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18160..19557)
                     /locus_tag="ACHBB7_001276"
     CDS             complement(18160..19557)
                     /locus_tag="ACHBB7_001276"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9209236.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19589..20560)
                     /locus_tag="ACHBB7_001277"
     CDS             complement(19589..20560)
                     /locus_tag="ACHBB7_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9209237.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20934..21359
                     /locus_tag="ACHBB7_001278"
     CDS             20934..21359
                     /locus_tag="ACHBB7_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209238.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         6292 a         4501 c         3781 g         7242 t
ORIGIN      
        1 aaagttttct gaatagttgt aattgtctga aaatttttta tcattagtca ttgttaaata
       61 ttgctattta ttcttaatat aaattttgat tgtgaattta aaagaaagag gtcatttatg
      121 gaagataaaa aattgaaaaa acaacttagc tccagacata tcaccatgtt agctttaggt
      181 ggagcaatcg gtgcaggtct atttaaagga agtggcgaag cgattgggat cgccggccct
      241 tctgtattga ttgctttttt gattggtggg ctgattttat atatcgtgat gaaaggtctg
      301 ggaaaaatcg ttttaagcgg tggcgacacg catcacggat tatctggttt gatacgccct
      361 tatctaggtg ctcattctgc tgattttact gattgggtct actggtctat gtggattatc
      421 aatatcatcg ctgaagctgt tgcagctgct tcctttttgc agttgtggtt cccgcacatt
      481 ccagcctggg tatttgtttt tatgttggct gttttaacta cgatcatcaa tctttattcg
      541 gtacggttat tcgcagaaac agaatattgg ctggcttttg ctaaaatcag tgtcattatc
      601 ttactgatca tttttgctac ttatcttgtc ggtcagcaaa tgttgggcac aggcgttttc
      661 cctacccttc agcaattgac tgaccatggt gggtttactc ctcatggtat gaagggaatc
      721 atcagttctt tgcttgtagt tatctattct tacggcggtt ctgaactgat tgctatcacg
      781 gtaagcgaag cagatgatcc taaacgtgcg attcctaaag caatcaaagg tgtcatggga
      841 agaatcattt ccttttacat cattcctttg tttttgcttt tgattctttt tccttggaat
      901 actttagcag gcacgaatgt cagtcctttt gtgatggtat ttgaaaaaat gaatatccca
      961 tttgcagcgg atatcgtcaa ttttgtgatt gtccttgctt tgttctcatc tatcaattcg
     1021 ggtgtgtatg catcttcaag aattttgttc ttccgtttga aggatcgaaa aggttcctct
     1081 agaaaactag ctgttttaaa caagcatcaa gtaccgcaac gtgctgtttt attttgtacc
     1141 agtgttcttt atcttggtgt agctctttct tattttgtcg gtgacaaatt atttggctat
     1201 ctggcaggtt ccctttcgta tacagtatta ttgatctgga tcattatcag tgcggcatcc
     1261 tttgtactgg cactaagaaa aggaacatta tgggataaag gaatcagctt ttttgcatta
     1321 gctgtcttag gattgatttt ccttggtatc ctgttcacga actccattgg ggtaactgta
     1381 ttgactggcc tgctttacct gttcatttat ttcagttatc aaaaaaagaa tgatgcgttc
     1441 gtattgacga atgaatgaaa tcgatagaaa attttatttt atttatgtgg aatagatgga
     1501 gctattgaag aaaggagttg tgacaaaagt cttgttgcaa ctcctttttc cgaataaaca
     1561 gtctcatgac ctcttggtaa acggtgttca aaaaccctct gtgcaaaata attgattgtt
     1621 ctccatgctc atgacatgat agaagaaagg aatgaaaaaa aggagtttgt tttaaatgaa
     1681 aaagtattgg aagtttttgt tgatgatcgg tgtttcaacg gtagttatgt ttggtatgat
     1741 gtacttgaat gtctatgcgc tcgatcatat cttcttcagt cgtacacgag tattcatggc
     1801 gttgatgatg ggtgcagtca tggcagtcat catgctgttg ttcatgtgga aaatgtatga
     1861 aaacaaaaag ataaatgtta tgatcttagc cgctagtctt cttgtttttg ccggttcctt
     1921 gtttatggta cgaagccaaa ctactgtcaa cgacacagct tggatgaagg ccatgattcc
     1981 tcatcattct atcgctatcc tggcaagtaa acgagctgat ttaaaagatc ctgaagtaaa
     2041 aaaattagcg gaagaaatca ttcaggcaca agaagaagag atcgacaaaa tgaaaaaaat
     2101 gatcaaagaa atggaatgac cattatccgt ccaaataaaa aaagaggatg ttccagaagt
     2161 tatcagcttc aagaaataag aagaagctac ttctggaacg ccgtgaataa ggggttgtga
     2221 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagcttc tcctattttt
     2281 cgtttggtta ggcttattac cgcagatgca agcttttgaa caccgtttat taagaggttg
     2341 taaaatcagc gttctgacct gagtattaga gagacaaatg gaaaaatagt tcctcatatt
     2401 ttgccatttt ttgagctaat accgagggtc agctgattga acaccgttta tcgagaggtt
     2461 gtgaaaaagt cgcttagttt cgcgtattaa gaaaaatttt gtgaaatggc tttccacatt
     2521 tttgtataaa tttggcttaa taccgagaaa ctggcttttg aataccgttt attcgtaaaa
     2581 gggattgtga caaaagtctt gtcacaatcc ctttttatgt ttttattgtt ctaattgcgc
     2641 tgtcttgctt actgcttctt gcggtttcac aaattttttt gagattcccc aacctgtgaa
     2701 ggcactgacc atcgaaaaca caggagaaag caaactgaaa aatacgaatg gcaagtaagt
     2761 gagtgtagga ataccaaatg tacttgctac gaaacttccg gcaattcccc aaggcactaa
     2821 gtagttgatt actgttccac catcttctag cgttctacct aatacagttg gatcaaatcc
     2881 tgctttttca taagtttctt taaatgcatt accaggcaga ataacagata ggaattgttc
     2941 accaataaaa atatttactc cgatacttgt catcaatgtt gtaaaaatca aatttggtgt
     3001 gttcgtcatc tttcttgata ctcgttccat cacggttgag atcaagccaa attcgatcaa
     3061 caatccacct aatgacaaag ttagaataat caatgacaca gtaggcatca tactttcgat
     3121 tcctccgcgg ctaagcagtg aatcgatttg ttggtttcca gtatttgaaa caaaccctga
     3181 ttcgatgacc ccagccaact tcgttaagga agtttgtggg ttttgaataa aaatcattcc
     3241 tactgctaca gcgatattca acaaaatcgt taaaattgcc ggcacctttt tccatgcaca
     3301 cagtaacatt aaaaataaag gaatcagtga ccagattgaa atagtaaagt ttgcttctag
     3361 aacatcagtc acttcacgaa tagatgaaag acttgcggct ttacttgtgt gtcctaaaat
     3421 catgaataaa aagaaagaaa caacaaatgc cgggacagtt gaccacatca tatgcttgat
     3481 gtgtttgaac aaatcggcat caacaatagc tgctgctaga tttgttgatt cggataatgg
     3541 agacatctta tctccaaaaa cggcacctga aacaatagct ccaacaacca gcgctggatt
     3601 taagcctagt gttgtcccaa taccgaaaaa tgcaatccca atcgttgaca taacagtaaa
     3661 ggcactccct acagcgctac ctacgattgc acacacaaca aatacagaag ggacaaacca
     3721 ctggacactg atcaatttga acccaatgac cattagagtt ggaatgatcc ctgcttgtat
     3781 ccatacagca atcaatgaac cgactaaaat aaaaataaag attgggatga ttcctggttt
     3841 gatgccattg atgattcctt cgtgcagcca atctgtcggg tatcttttta tcactgcata
     3901 caacatgacc attccgatcg ctgctaagat cgtgatatta ggtgagacct ttaatccgac
     3961 aacgccaatc gcaatgccta aaacaataac cattaataca agtgttgctt ctaaaaaact
     4021 tggtgttcct ttttttccca tgataatctc tcctttttta gttgttctta tgttctaaaa
     4081 aagttaagat atcggattac tccgccgcaa ttcatccgcg attcctcctt tataaaaata
     4141 aaaagacctg ttttaaacta aggacgaatg actccgcggt accaccttaa ttaaaaacag
     4201 atctgttttt cactttcaaa cttttattca aatgtatgga ctttccatgt aattcgtcaa
     4261 ttaggcttga tcggttcaca gcgaccaccg actttctgaa caaaacacct aaccgctact
     4321 gcgtgaaaag ttgacttgta cgaagtttac cttccataag atcatttgtc aatgaaaaag
     4381 aaaatatttt ttcatttttc tgaaaattat ttttataact ttcacacaca agattatttt
     4441 ttactagaaa gtagcgccct cttgtttttt tcttgctact atttcttgat gttcttgctt
     4501 gattctttct aatagctttg gctctttcaa cagatcaaga gctgtattgg cgagtagttc
     4561 tgctgcaatc gcaatagaag caaggccttt gtcactttta gcagcagctt tgaattcttc
     4621 tgtgtgtccg gcaatatatg tctcagagat tgctaccgta ggctggattg ttggtatgac
     4681 ttggctgaca tttcctacat cagaagaacc gagacttgtt ctttgttccg tttctatttc
     4741 ttcatctgga acacctgctt cttttgcatg ctggaagaat aagtcatcaa aagaaggagt
     4801 aacgatgaca tcatcaactg cattttggaa caatccaaac tcaaaagtcg tgcccgtact
     4861 tatcgcagca cctcgtacga tattttccac tttttcatat actttatcta atgtttgccg
     4921 atttcctgca cgtaaataga aacgtccagc agcgtaatct ggaatgacgt tggcagctac
     4981 tcctccgtct gtaatgatcc catggatatt gacatctttt ggcaattgca agcgtagagc
     5041 gttgattcca ttaaacactt ggatcaatgc ttccaaagca tttactcctt tttcaggtgc
     5101 tgctgctgca tgggaagcga caccgtaaaa tttgatgtcg actggatcat tcgctaaaga
     5161 ttctgtcgtt ttcccataac gaaaagctgg atggacgcat aacgctacat cgacatcctc
     5221 aaaaaaacct tctcgaacaa agctgccttt agctgatccg ttctcgcccc cttcttcccc
     5281 tggggtgcca tatacacgga tttctccacc agtttcatca atgaattgct tcactatgct
     5341 ggcggctaat acggagtaag taccaaacaa attatgcccg catgcatgac caatacctgg
     5401 taaagcatcg aattctgcca aaaaggcaat tgtgggaccg ggtttattgg aaatataacg
     5461 tgcatcaaag gctgttcggt gtccagctac ttctttttct acccaaaacc tttctttttc
     5521 taattgttta atcaacgctg ctgaggaata aaactcatag ttacttactt caggatgttc
     5581 gtggatatct agagcaagtg cttgatattc tggtaaacga tattggatta gatccttcaa
     5641 ttggtttgtt gagattgttg ttgtcataaa aaattcctcc tatgctagtc atttttctta
     5701 ttttctcccg aatgtttccc atgcaggcac gcttgatcct tttgatgttt tctcgattac
     5761 tttgactgtg tcttctgttt gataagcgtc tactattttt tgatacgttt tattgttttc
     5821 atcttctttt cttgcaacga tgatattcac ataaggacgt gcatggtcat ccactggttc
     5881 taagaagatg gcatcagaag caggattaaa tccagcatct acagctacac cgctattgat
     5941 cactgaaatg tctacatctg acaaagcacg tgctgtttgg gaagcatcta attcagaaat
     6001 gtctagattt tttctattct cggtaatatc agaaacagtg ggcgcttgtc cttttgcctt
     6061 atccactttg atcaatccag cagtttgcaa taacagaaga gctctgccgc catttgtaac
     6121 atcattaggg attgctactt ttgctccgtc tttgatatca gctacatcct tcaaagtgtt
     6181 agaataaatt cccagcggtg cattgacagt atttccgatc gatactaaat cagttccgtg
     6241 ttctttattg taattgtcta aaaagaattg atgttggaaa gaattcaaat caatcgatcc
     6301 atctgctaaa gccacatttg gttgattgta atctgtaaat ttcacgtatt ccaaattaat
     6361 gttctccttt ttcaaacggt ctttcacgtt gtcccataca tctgtatctt ctccaataat
     6421 ccctagtttt acgatagttt cttttttgtc agttgtatct gcttttcctt ggctacaccc
     6481 tgccaaccaa ataattgcta caaatgtaat gactacaaaa cgaataattt ttttcatttt
     6541 ttctcctcca ctttcaatgt gtaatttttt taatcagttg attactgata aattgactta
     6601 aaaaaactaa gattagaatc aataatgtag ccacaatcgt tacatctgtt tggaatcgat
     6661 tataccctct cgatatagca agattcccta atccgcctgc acctactgct ccagccatgg
     6721 tagtcagtcc gatcacgttg atgatagtca acgccgagac ccgaatgata ccaggcaaac
     6781 cttctaacaa atacacacgt aaaatgatac cgattggact ggttcccatc gcttctgccg
     6841 cttcgatcac cccaggatca acttctagca gtgcgttctc gatttgacga gcaaaaaacg
     6901 gaacaactcc aaaaatcaat ggcactaaag ctgctttcgc accaatcgtc gttccaacta
     6961 agaaacgagt agtcaatgct aaaagagaaa gcaagataat gaaagggatg gaacgaatga
     7021 tattgatcag cttatcaagt atttcaaata gcagacggtt agctagtaat ccattagggc
     7081 gtgtcactac taacgtcaca cctaagatga ttccaatcat cccgccaatg aatgctgtcc
     7141 aaaaagacat atatagtgtt tgcttcgtcg cttcgacaaa ttcatccgga atcaaataaa
     7201 cattgggcaa atatgtttta aagaaatcag tcattttatc ttcctccttc gatcactttg
     7261 atacgactat ctaaatcgtt tttttgtaaa atatttattt ttacaccaga tttgatcaaa
     7321 taatccaatg cttttttccg ctgtttggtt tctccagcta aagtaacgat caaactccct
     7381 aacggcgttt cttgtaataa ctctacattg ccatataaaa tattcgctgt tacttgatat
     7441 ttgctgtaca gatgagcgat tagaggttcg ttcgtctgat ttccaatata acttaattcc
     7501 accagccatt tattggcgat ctgatcggca aaagcagaat gggcaatgat cgtttctaat
     7561 gcttggtcaa gatgagtagc tgttcgaata aaatctttgg tcaattcttc ttcaggattg
     7621 ctgaagatct ggatgctgct acctttttca acgattcttc catcttccat aaccgctacc
     7681 ttgttgcaga tttctttgac aacttgcatc tcatgagtga tcaggacgat tgttaaacct
     7741 aactggcgat tcaatttttt taacaatgca aggatctgaa gggtcgtttt cggatcaagt
     7801 gcactggttg cttcatcaca taataatacc ttaggttcat tcgccaaagc tcgtgcgatt
     7861 gctactcgtt gtttttgtcc ccccgacagt tgtttaggat aagcagatgc tttttcttcc
     7921 aaaccgacaa gttccagtag ttcattcact ttttgacgac gttcctgttt cgattttcca
     7981 gaatacttta aagaaaaatc aacgttgtca aaaatagttc ggctttccat tagattgaaa
     8041 tgctgaaaga tcataccgat ggcctttctt tcttttcgca attctttggg agagagctga
     8101 ctcagattcg tctgattgac aatgacttct cctgctgttg gtttctgcag caaattcatc
     8161 acacgaacta acgtactttt ccctgcacct gagtaaccga cgattccata aacatcgcct
     8221 ttttcaatcg ttaaatctac agaatctact gcctgtatct ctttttcttt ttgtttgaaa
     8281 catacagaaa catctcgcaa ttcaatcatt aaaattcctc cttatctaaa aaaagcgtcc
     8341 tcaagaaaat gaaaatgtca ttttcttgag gacgcttttt tgcgtgttac cacctcatgt
     8401 tcaagctgtg ttcacacaca actcctcttc aagtacgatc atactctagc aagatatcgg
     8461 tcgcagccgt catcgcctta catctatgct cgggatgaat tgctcgatgg ccattttcca
     8521 atagtcgttc cttgcttctt ctcagctacc gaagctctct gtcaaaggtt cttctatcgt
     8581 acttaccatt tcaatgcaaa taatattcaa aaaaaaagtc cttttactaa cataaactat
     8641 gtcagtaaaa ggacgacgta tcgcggtacc accttttttc ggaagtttcc ttccctcaag
     8701 cccaacatct caaaaagaga gattggcgtt cgttttaacg gtcgaacagt tccgaatttg
     8761 cctacttatc gacaaattat tctccaaggc cattttcagc ttttcttact tgactcttct
     8821 caccagccag agcctctctg caaagtttcc aaacttactt tccttttcaa cgaaatcgat
     8881 attttgttgt tgggattaat ctacatgagg aaaactgaaa agtcaagtac gaaattcaaa
     8941 ttattttttc tatatttatt tttctgtttt tttatttaac atttcaataa cagttcgaag
     9001 atcttttcct tccgcaagaa gtttcaattt ttccttttct tcgacggtat aatttcggac
     9061 aaaactggaa tcaggtatca aaacaacgcc taatcctgct cgtgaagcag ctgttgctcc
     9121 tactaaagaa tcttccactg caaaggctga actagcttct aatcctgttc gttccaaagc
     9181 tgtcagataa ggaatcggtg aaggcttgtt ctccgacact tcgtctccga atgtaaacgt
     9241 gtccaagtaa tcagctaatt taaagtgatc caagatatct gttgctcttt tcgtaacagt
     9301 ggtcgtagca atgcctagtt tcatatgacg ttcttttaag tctgccaata tttcttttgc
     9361 atatggtttt aagtggatgg ctccgcttgt cagctgttga taaaaatatt cttcacgctt
     9421 cgctcggact tcctgtactt tttcagggct acctgctgct ttcactaaaa tatcaaaact
     9481 ttgtttccag ttttttccgc cccaagcgtc aacgatttct tttgggatat ggatatcaaa
     9541 ttgctgaaat ccccaaagcc agccatcacg atacgttttt tctgtgtcaa ctaacaaacc
     9601 atctaaatca aatattgcac ctttcatcta tacattaatc ctttccaaaa caatacacat
     9661 agtttagcac gactggtcca aaaagaacag aagcaaagaa ccagccttct cgtttataag
     9721 agttcttgga atcgacggat atagattttc ttcacagctg tcacaagcgc cagataagcg
     9781 atgactgtag caaataacca taaccagtaa ttgccaggaa gtggcatcaa gcctaattca
     9841 gcaccaaaac ttgtaaaagg aaggacagaa ccgacaataa ttcctaatgt agtgataaca
     9901 aacatggcaa atgttgcgtt gctttggata aatggtactt tcggtgtacg cagtgcatgg
     9961 agcactaagg tttgtgtcca tagtgattcg acaaaccatc ctgcatggaa taatgcgatg
    10021 aaaatcactc gctgtgaact atccaatgta tggaaacttc cgcccactac agcaggacaa
    10081 ataacaaaat acatcaacaa gtaagtagta atatcaaata tcgaacttgt tggaccaaag
    10141 taaaccatga attccccaat actagatgca ttccattttt ttggctcgtc caaatattct
    10201 ttatccatcc gatcccatgg caaggaaatg caggaaacat catagatcag attcaaaaac
    10261 aaaagttgta aaggcagcat tggtaaaaat ggcaggaacg tacttgcaat cagcacggaa
    10321 aacatattgc caaaattaga acttgctgta gccttgatat atttcatgat attcccaaaa
    10381 gtttctcgtc cagaaagaag gcctctttcc aaaatcgtca aatctttttc taaaaggatc
    10441 acgtctgcag attctttcgc aatatccaca gctgtatcta cagaaatacc gatatcagcc
    10501 actttcattg caggagcgtc attgatacca tctcctaaaa atccgacagt atgaccattt
    10561 ttacgtaaag ctgtcgtcaa acgtgctttt tgctgaggat tcaattttac gaatacttca
    10621 tggtcttcag ctacttgtgc taattcatga tcactcattt ccgagatttt ctcaccggta
    10681 accaattcat tgatctctag acctacttga cggcacactg aacgtgtaac taattcgttg
    10741 tccccagtca gaacttttac agctacacca tgttctttca gagctttcaa cgcttgtttc
    10801 gttgtttctt ttggtggatc aaggaaagca agataaccaa tcaaaaccat gtctgactca
    10861 tctttgactg aaaattctcc aacgacgctt ggattggtct tttgtgcgac accaatcact
    10921 cgtaagccat cttcattaag tgactgtact tttgcaagta cggcttcttt tcgttctttt
    10981 gttaatggtg taacagtacc gccaagatca ataaatttag aaatgttcag catttcttca
    11041 atcgcacctt ttgtgatcat ttgtgtttta ccagctggat cagcaaccac aacactcata
    11101 cgacgacgtt caaaatcaaa aggaatttca tccacttttt gataattgat cttatctgta
    11161 tctaaagttt ctgttgcttc atcaatgata gccacatcta acaaattttt cagccctgtt
    11221 tgatagtagc tatttagata cgcatggcgc aaaacacgat catcttcatt tccttcacaa
    11281 tccatatgat attcaagaat gattttatct tgtgtcaatg tccccgtttt atccgtacat
    11341 aacacatcga ttgcaccaaa gttctggatg gcattcaaat ttttgatcac tgttcctttt
    11401 ttggccatca cacttgctcc tttgaccaaa ttcgtcgtaa cgatcattgg aagcatttca
    11461 ggtgttagac caactgctac agatagtcca aaaagaaaag cttctagcca atcccctttt
    11521 gtaagcccat tgatgacgat aactgctgga gccatcaatg acataaattt gatcagtagc
    11581 atggatgttt tatggatgcc taattcaaag cttgattgaa ttggtttttc agatagcgtt
    11641 tctgctacat gaccaaatag tgtttcattt ccaacagaaa caatgattcc ttccgcactt
    11701 ccactgatga cattacttcc cataaagaca agattttcgt aacttgtttc gctttgctga
    11761 tcgtcttcac atactttcgc atgcttttct actggatagc tttcccctgt caatgctgct
    11821 tgggaaatga ataaatcctt tgcggacaat aaacgcatat ccgctggtat catatcccct
    11881 gctgaaagcc gaacaagatc tccacagacg atttcttcca taggaatctc tgtgtacttt
    11941 ccattacgtt ttacagctgc cgttactttc acaagtgatt tcagtttttc agccgcttga
    12001 ttggaacgta cggactgaat caacgtcatt gtcccgctga cgataaccat agtgaagata
    12061 atggctacac cgaaaagatc tttatctcca ggagctgcta atacatattc cgtgatgaat
    12121 gaaataattc ctaatgcgat caacactaat gtaaatggcg tgatgtatgc tttcaatatt
    12181 tctacaatga atggtgtttt tttgccatat gaaatcgcat tttcaccgta attttctctt
    12241 gaacgttcga tttgttcttc tgtcaatcca tttgctgatg catggaatag ttcaaataat
    12301 tcttgtttcg tttttccagc aaaagctgca tagatttttt gtgtatcttt ctttttttcc
    12361 atctcaatct ctcctttgtg tccaaaagac agctgttctc gctgcttttg gtctttagct
    12421 attcatcgga tcgatagaga aaaaactgaa aggggctgag aacagataga taatagccct
    12481 ggcctttcct ctagatcctt gtttttcgaa tgactgtcat ccatttgttc tcatctcctt
    12541 tactaagtaa ttttaattca tgacttccaa tcatttttcg aatagttatt tttccaatta
    12601 gaccacctcc tttttatttt tcaccgatgg ggatttgcgg caacaaaaaa acctctcgcc
    12661 aaatactcag cgaaaggttt gaaaaagcat attaaaaaaa gcttctcaga tctcatcgtt
    12721 gagttttgac actgtacaac gtaaaaagca gatacctgct tttagctacc ttatgaaaag
    12781 ccttatctcg acaattcctg ttctacccat tggcgtctct cgacgttttt gggcagtagc
    12841 ctatgtttgt ataggagcct cacctaacaa atcttattcg gttttccttc gttaatataa
    12901 caactaaaaa agaggatgtc aaatcaattt tccatgtttt tcatactttt tttaaaatac
    12961 acacgttata ttcctgtctc acgcggtttt atttgtctca ttttccacct cccgctataa
    13021 tagaaatata gtaggaaacg aggtgctgtt atggaaagaa cacttgttat tatcaagcca
    13081 gacggcgtga gacgccattt agtcggaagc atcatccaac gttttgaagc aaagggactg
    13141 gccattgctg aaatgaagtt tgaaactatg actcctgaat tagcaaaaga acattatcag
    13201 cacttgactg agcgcagttt ttttgatgaa ctgatcgatt acatgacttc cggtcctgtc
    13261 gtttatcttg tgctggtcgg cgaggaagtg atcgatatcg tccgtaagat ggttggagca
    13321 acaaaagctg ccgatgctgt tccaggtacg atccgaggag actacgcgct gccggggact
    13381 gaaaacatca ttcacgcttc tgattcccgt gatgctgcgg taaaagagat cgcccgcttt
    13441 ttcccgccat tcgatataaa caaaccgtct gaaaaagcgg cgaagtgata caaattctaa
    13501 actgctggtc tagttgctgg gttcagcaat ggccagcagt tttttctcta aggacctccg
    13561 ttgattcttt cctaacatat attcgagcac tgtgaagaat tcttggtagt ctttcgtctt
    13621 ttcttcccct aaagcagcag aaatcaatct ttgataataa tctgccatca atgtttcata
    13681 gatcactgta cttctttctt tatgtgctaa gctccgtttc atatcttcat tcacactacg
    13741 tgcctttttc aaccgaccat tttctatata aaacgaagtc aggttcatta aaaaagtaaa
    13801 gagatactgc tcatgattgt tcaactgtgc taatcgtcgc attctttttt tgattttcaa
    13861 ataattgttc tcaatgaacc gatcactgaa tatccaagga aaatcagcta gaatagatag
    13921 ttcaaattga ccatagtctg gtatctgcat caagtattct tcaatgattc ggcattcata
    13981 acgtaaaaat tcaaattctg tctcagatac ttctgttttt tcgaccatgt gaagattcaa
    14041 aaccaaataa tacagatctt ctgttttctt aaaatgccta cggttattgc tgatctcttt
    14101 ataaggatca aagctgtctt cttgtacccg atctagcatt cgtctttttt tctttaactt
    14161 tattggttca tccagctggt ttcgcatcca ttcatattct gcaagtgtca tgttcattcg
    14221 ctctaaaaat gatctcaatg ttacaaatga catttttttc actgaatttg tctcaatccc
    14281 gactagagaa gtcttaaaac agattccttc cgctaattgg gactgtgtca acttcttgtt
    14341 gactcgaagt ttcttgatca attcaccatc atacataata gttctccttt ttaatttaaa
    14401 tttttccaca ttcaataaat agatacgtta aaaaggaaaa atacacgaaa aaaataaaaa
    14461 aatgtttttt tcataaaaaa taccaacctt caagcattag aatgttgtct aactactgaa
    14521 ggttggtttg attgtttatt gattattttt ttgctgttca acgtctgttc ttgttactgg
    14581 atataagttg gttgttgttt tattaccatt ttttgaaaag acacttgtcg aatgttcgcc
    14641 tttttgcttt tcgattgctt cattacgttc cgcttcattt ttatagtcat attttttcgg
    14701 atcaatcgtc tcaaatccgt ttggcacata aaaccgcaag agattttcct gattcagctt
    14761 atctgaaaga cttaacgcag tcttgacttt ttcctgatct ttggagattt cctcatcgat
    14821 tcctgctgct ttcgtatcga tgatttctcc tgtctgatta ttgtaagctt ttcctcctag
    14881 gacggtgtat ttgggtgtca caaagttccc atttcgaaaa gcaactactt gatcatgctg
    14941 agaagataat aagtctgtac caaaatgaag ataatcttta tcatcgattc ccaatagatg
    15001 tagtaatgtc ggcaaaacat cgatttctcc accgtattca tgattgactg tccctttagt
    15061 ataaccaggc atatggatca tcaatggcac tcgttgcatc tgtgcgttgt caaattcatc
    15121 ccatgtatct ggatctttac caagagcgct tgctaaatct ttattatctg tattcgagat
    15181 cccaaaatga tccccgtaaa tcacaaagat tgaattttgg taaataccag attttttcaa
    15241 atacgtaaaa aattgttcca atgattgatc cagataatgt gccgttcgaa cataattatc
    15301 tactgtgcta ttgccagtat tcaaagatgg aaagtcgaaa ttcttgtcat ccgtataata
    15361 aggcgtgtgg ttggttacgg aaagaaattt cgtgtaaaac ggctgttgta gatgctcaag
    15421 atattgggca gattctctga ataaatcttt atctaatatc ccataaccta acgtttcgtc
    15481 ggattcatca aaataagatc gatcaaagaa attgtcataa cctaagtttt tatatacatg
    15541 atctctattc caaaaactag ctacattccc atggaaaaca gcgcttgtat agccttgctt
    15601 ttgtccaagg atcgctggtg cggcttgaaa gacattgtct gacccaagtt ctgtgaataa
    15661 tgagccttga ggtaaaccaa atgtgccagt ttccaacata ttttcagcat cacttgtctt
    15721 tccctgtcct acttcatgaa agaaattgtc aaagctgatc gttgcttgat tctggaaaat
    15781 actatttaaa aacggtgtga cttcttgtcc gtctaccttc atattaatca aaaactgctg
    15841 gaaactttct aaatggagaa cgatcacatt tttcccttta gcgatcccaa atgtttcggg
    15901 atttggctct gcataatgtt tctttgtata atctaaaact tcatcaatcc cattactgct
    15961 tgcatgcgcg cgaacactgc tagtcattcc tgttttgatt ccgtcataaa tagtatacgc
    16021 atcgattcct agatatttga caatatatga acggtcaaat gttcgctgca gcaattgtgg
    16081 acggttggct tcgctcagcg ctagatttcc tgaaaaaata agccctgaaa gacaggtaac
    16141 agcgatagcc atcggtttgc cgacagggtt gcttttgata ggtattttct tgaccttcaa
    16201 ccaaaccagt agcccaatga acacagcgat atccagccag tagatgacat catggggctt
    16261 catcaaagaa aaggagcttc ctgacaatcc ttgcgacact ttcgaaaagc ctaatacaga
    16321 tttgactgta atgaaatctg tgaattcccg ataaaaaata atattcaagt acaacaaaag
    16381 cgtatttaaa atatcaatga tcaaaagaat agctaacgct ggctttggct ttttaatata
    16441 caaagaaagg ccaaaaaaca ataaggtagt cgcaattggg ttgatccaca agataaagta
    16501 ctggatcgta ccttccacac ctaatgaaaa atctgcatag taggcaatga tcgtcttcag
    16561 ccagaaaaag aaaacagctg tcaaaacgat tgctaaacga ttttggcaca attttttaag
    16621 ttgtgtcacg tatatcactt ctctctattc acattttttc ttattttgct cctgaaaaat
    16681 gaaaatttcc taaaaaagtt gttatcttaa gattttttta ttctttctaa agattattcc
    16741 gtccactttt cacagcataa aaccgctgcg atgtaagcga ttaaactaat gaagagataa
    16801 aagtcttgtg gtgaccgctt cactccacgg tattcaaaag ccagtttctc ggtattcgta
    16861 caaaaaaaga ggctgtgaca aaattcttgt cgcagctcct tatttcttga tgcaggacag
    16921 ctttttcaca acttcttcct taaacggtgt tctcctgcca actcctcggc cacaagtcac
    16981 aatatttata aaacatgaga agctgttttt tgaaattgtt ccttgtggct cagagttaaa
    17041 cggcaggctc aagacctctt gatacacggt gttcaatcag ctgaccctcg gtattagatt
    17101 atgtcaggac gctaatttta caacctcttg gtaaacggtg ttcaaaagct tgcatctgcg
    17161 gtaataagcc caaccaaacg aaaaatataa ggagctattt tttgaaattg ctccttgtgg
    17221 ctcagagtta aacggcaggc tcaagacctc ttgatacacg gtgttcaatc agctgacctt
    17281 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg tttctctaat
    17341 actcaggtca aaacgctgat ttcacaacct ctgtttttca ttgtccatag gattgatatt
    17401 gtccttgtga tgactcattc gtaataattg aggcttgatt ttcttcactt cccgttatcg
    17461 tttgtccact gccagaatct gaaggacttg ttcctgtcga ctcgtttttt tgagaagaga
    17521 cagttgtttt cttgacttgt tcatttgtct ttttccattc cgctaaacga tcggcgacca
    17581 cttttttgtc tactttttca gaataagcgg tgatcacttt tccatttttc aaacgggcta
    17641 ctttgtcgcc atcatgagtc aaaagcaaaa cggtcaaatg cttgtcttta aattgatatt
    17701 gtctgagaga aatcgcacta agtacgtcat cgatattaat aaattgattt tcttcttttc
    17761 cttttggcaa ataaatgtcc aattcgcctt tttcgtaatt ggcagttatt ttatactccg
    17821 cccaccaata agttgagaat tcttttttga aattatctac tgattctttg cttgctacga
    17881 ttttttctgt ttctttctga gcaagatctg ctgtcatttt tcctgaaaat gctgcggata
    17941 aaataagcag taagaaaaaa ccgaataacc aaaagagaag aaacggtctt tttttcctca
    18001 tgctttcctc cactcctatt taattttgac tattctatta tatacaaaaa atgaaataga
    18061 aagacaaaaa agttgaaaaa gatcgtaaat aattgaactt ctttttcaca aaatggtaag
    18121 aatggagaat ttcacgcatt tgctctcaac tattctttct taagcctcaa atccgttttt
    18181 atctgatacg tctttgaacc aatgacccga ttttttgatt gttctttttc catcttgtgc
    18241 taagtctact gaaataaagc catatcgatt cttatatgca ttggtccacg accagttatc
    18301 catacatgtc cacatatgat agcctaaaca attggcacct tcttgaatgg cttgatggac
    18361 atatttcaga tgagattgga caaattcgat ccgataatca tcgtggatca tgccatcttc
    18421 tttgataaat cgtgcttcgt tttctacacc cataccgttt tcagaaataa agcagtcgat
    18481 atttccataa tgatctcgta cattgatcaa aatatcatag attccttgtt catagatctc
    18541 ccatccgcga taaggattca ttttacgacc aggcatttca taattgtcga aataatcctc
    18601 aggcattgga ccgtttttag tatcgattgg tgtttctttt gcttttaccc ggcgtggctg
    18661 gtaataattg atacctaaaa gatctactgt attttctttg ataaccgtca aatcctctgc
    18721 atggatcaca ggcatgagat caagttcttt tactatctca atcaattcac ttggaaactc
    18781 gccttttaca gctggatcaa ggaatgaacg attgaagaac gcatctgcta tttgcgctgc
    18841 tttaacatct tcagggtttg attcgtctct tggatagctt ggtgtcagat tcaagatgat
    18901 accaataaca ccaccttgct ccatttcatg gtaagatttg atggctaacg ctgaagccaa
    18961 cgcttcatga tagccaactt ggatcgcttt tttcatatct gactcttctg gataatgcca
    19021 tcgatataaa taacctgctt cgactggaac gattggttca ttatgagtaa accatttttt
    19081 tactttcgaa ccaaacaatt caaaacaact tttggcatat ttttcatagg cttcaaccgt
    19141 ttctcggttc aaccatccgc ctttttcttg cagatacatc ggcatatcga aatgatacag
    19201 attcacaaag ggttcgatac cgttttcaat caactcatca aaataacgat gatagaaacg
    19261 aacggcttct tcgttgattg ttccagattc atcaggtatc aaacggctcc attggataga
    19321 tgttcgaaat gaattatgcc cagtctgttt catcaactgt acatcctctt gatacttcgt
    19381 ataaacttgc gaggtttttt caggtcctac ttgatcaaag aacttttgcg gttctttttt
    19441 gtaccaataa tcaaaaatgt tctctccttt tccatcgcct gaaatccttc cttctgtttg
    19501 tgggccactt gctgcagaac cccaccaaaa atttgctgga aattgatatt ttttcatcaa
    19561 agcacctctt tttctttttt agttcgtttt agttgattcc atgtatgata ttttccatca
    19621 caatcacttt ttcattttct tcctcttctt ttttgattcg ttttaacagc tgttctgctg
    19681 ctcgttcccc gattttatct gttggtggcg tgatcgtgat tttcggaagg atactcagat
    19741 cgtttttatc taaataatca tagtgactga acgcaatatc cttcccgatt ctgacacctt
    19801 cttgattgaa acattcgatg gcaccaatcg acatattgta attgctgaca aaaatagcag
    19861 ttgccccttt ttctaataat gcttttgccc cgtaatagcc gctttttcta gaatagtctc
    19921 ctgtaaagac ctctaattgg atcgatggat cattttggac cgcttctttg gctcctgcta
    19981 aacgttctct tgctacgtag tctgtttgag tggcagcaat aaatccgatt tttttatgtc
    20041 cttgttcacc atgatgagcg atcacttttg ctacactttc tcgatcattc actaatattg
    20101 tatcaatacc tgctggtcct tctggacaat tcaatgcgat aactggaata ctcatttttg
    20161 tgagcatttc aaatccagac cactcttctt cggataagaa gacaatcagc ccatcgatca
    20221 catgcttcat caggtattca attttttgtt caattttttc tggctgatcg gcaaacccgc
    20281 ttaataataa gctataccca ttttcttcag ctactctttc gatccctgag acaacttccg
    20341 acccaaaacg gctggaaatc ccgtttatca aaagtccaat agagaagctt cgattattct
    20401 tcaacccttt agcaatgatg ttttctttat agtccaacgc tgaaatcgct tctgctattt
    20461 ttttcatgtt cttttccttc agttgctgac cattcaagta acgggaaact gttcccactg
    20521 aaacaccagc catcttagcc acatcacgga tcgatgccat acattctcac tcctgccagt
    20581 tacatttgat ttctctttct tgttgttcga ttatgatgac cgcattcttt gcatagtgtt
    20641 gcttgtcatc aatatccata taactaaagt cttcagtaga gcaacttcct ctcactatta
    20701 attatactta aacaagtatt tgaaccggtt catttttaat atagcatttt tttaaaagat
    20761 tgcaacactt ttttaaaacg ctttctgaaa gtggcggatt ttttgccttt ttaaaatttt
    20821 attctaaaat ttactctatt tgtgggtgtt ttgtgcgttt ttctcttcca tccgtttgaa
    20881 gttttacatt ccaatggtat aatttcttta tcctatcaaa tgggggaagt attttgaaga
    20941 aattaatcgt actatttatt ttgtctgcgg gacttttgac attgactggc tgtgatttgt
    21001 ttggcggctc tgaccaatcc tcggacgaaa caaccgattc ttctgtctta aaagcggaaa
    21061 aagcagatac caccaacagt gtatatacgc taaaagtacg taaagatatg gatttatccg
    21121 cgggacagtt gacatttaca gataaaaaaa tggaatggac acgcacgtat cagacacaag
    21181 gaactgattc ttctgaatca tctgaagaaa aaacagtgct gacagatatc aagatcacta
    21241 ccaaagatga cgaatatatt atttcaggaa aagaaaataa aaaagaaaca acagtcatat
    21301 ttacaaaaat cggtaattac cgtttgaagg atgaggatgg caacatctac agtctatagt
    21361 gccgtctgtt ggatcttctt ttgtttctcc ccttcttttc tgctatagta aagaagcaag
    21421 atgattgtaa aggagacgaa atggatgatc gatagtcctg attattgtac atgtaataac
    21481 aacaaaaata tccatacaga atttaatgac caaggaagct tgagcatatg cgacaactgt
    21541 cacaaactag tcaaagattc atttaaaaca tttgaatctt atcctgttca aaattatgta
    21601 aaaagtagaa gaagataaac aaatccaagc aaaactcact atggctgatt tttatgaact
    21661 tcaaactcag cttcttcctt tgatcaagct tcatcagtaa aacaaacagt ggatcatttc
    21721 agtttacaaa tcaaagagta aagatttatt tatttcaaat atttgaatat aaaataacca
    21781 aacgatagaa aggcttattc tacatcacgt agtaat
//
LOCUS       DAYYKN010000042        21352 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000042 DAYYKN010000000
VERSION     DAYYKN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.042"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..868
                     /locus_tag="ACHBB7_001279"
     CDS             167..868
                     /locus_tag="ACHBB7_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9209239.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1477..2493)
                     /gene="tsaD"
                     /locus_tag="ACHBB7_001280"
     CDS             complement(1477..2493)
                     /gene="tsaD"
                     /locus_tag="ACHBB7_001280"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM9209240.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2490..2954)
                     /gene="rimI"
                     /locus_tag="ACHBB7_001281"
     CDS             complement(2490..2954)
                     /gene="rimI"
                     /locus_tag="ACHBB7_001281"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM9209241.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2961..3503)
                     /gene="rimI"
                     /locus_tag="ACHBB7_001282"
     CDS             complement(2961..3503)
                     /gene="rimI"
                     /locus_tag="ACHBB7_001282"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM9209242.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3487..4209)
                     /gene="tsaB"
                     /locus_tag="ACHBB7_001283"
     CDS             complement(3487..4209)
                     /gene="tsaB"
                     /locus_tag="ACHBB7_001283"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM9209243.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4400..5380)
                     /locus_tag="ACHBB7_001284"
     CDS             complement(4400..5380)
                     /locus_tag="ACHBB7_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9209244.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5404..6888)
                     /gene="galT"
                     /locus_tag="ACHBB7_001285"
     CDS             complement(5404..6888)
                     /gene="galT"
                     /locus_tag="ACHBB7_001285"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM9209245.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6900..7889)
                     /gene="galE"
                     /locus_tag="ACHBB7_001286"
     CDS             complement(6900..7889)
                     /gene="galE"
                     /locus_tag="ACHBB7_001286"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM9209246.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8137..8304
                     /locus_tag="ACHBB7_001287"
     CDS             8137..8304
                     /locus_tag="ACHBB7_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM9209247.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8366..10177)
                     /locus_tag="ACHBB7_001288"
     CDS             complement(8366..10177)
                     /locus_tag="ACHBB7_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM9209248.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10174..10539)
                     /locus_tag="ACHBB7_001289"
     CDS             complement(10174..10539)
                     /locus_tag="ACHBB7_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM9209249.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10702..11097)
                     /locus_tag="ACHBB7_001290"
     CDS             complement(10702..11097)
                     /locus_tag="ACHBB7_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9209250.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11115..12077)
                     /locus_tag="ACHBB7_001291"
     CDS             complement(11115..12077)
                     /locus_tag="ACHBB7_001291"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM9209251.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12077..12289)
                     /locus_tag="ACHBB7_001292"
     CDS             complement(12077..12289)
                     /locus_tag="ACHBB7_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM9209252.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12310..13008)
                     /locus_tag="ACHBB7_001293"
     CDS             complement(12310..13008)
                     /locus_tag="ACHBB7_001293"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM9209253.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13028..13570)
                     /locus_tag="ACHBB7_001294"
     CDS             complement(13028..13570)
                     /locus_tag="ACHBB7_001294"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM9209254.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13702..14706)
                     /locus_tag="ACHBB7_001295"
     CDS             complement(13702..14706)
                     /locus_tag="ACHBB7_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM9209255.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14703..15692)
                     /locus_tag="ACHBB7_001296"
     CDS             complement(14703..15692)
                     /locus_tag="ACHBB7_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM9209256.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15689..16495)
                     /locus_tag="ACHBB7_001297"
     CDS             complement(15689..16495)
                     /locus_tag="ACHBB7_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209257.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16661..17617
                     /locus_tag="ACHBB7_001298"
     CDS             16661..17617
                     /locus_tag="ACHBB7_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9209258.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17694..18212)
                     /locus_tag="ACHBB7_001299"
     CDS             complement(17694..18212)
                     /locus_tag="ACHBB7_001299"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM9209259.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18300..18449)
                     /gene="rpmG"
                     /locus_tag="ACHBB7_001300"
     CDS             complement(18300..18449)
                     /gene="rpmG"
                     /locus_tag="ACHBB7_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9209260.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18677..19123
                     /locus_tag="ACHBB7_001301"
     CDS             18677..19123
                     /locus_tag="ACHBB7_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM9209261.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19318..21213)
                     /gene="asnB"
                     /locus_tag="ACHBB7_001302"
     CDS             complement(19318..21213)
                     /gene="asnB"
                     /locus_tag="ACHBB7_001302"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM9209262.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6418 a         4533 c         3684 g         6717 t
ORIGIN      
        1 tcgcttcaaa acaaattatt caataccatt gtatattttt tacataataa caaattatat
       61 ttgtaaagta ttaaaacttg tttctaatgt tggtatgtta aaactttaca aattctttaa
      121 aaaaagtgac aataaatata gtgaacaata aggagaggac tttttaatgc gtgttaataa
      181 aatcgatttt cacttttcaa aactcagata tcaacctgat tttgaacatt tctcggatga
      241 agagttccaa cagatcaagg aacatactgt tcttcgttct tataaaaaag gacagatcct
      301 tttcgacgat ggtgatccca gacaacggtt gtatatcttg accaaaggac tcgttcgttt
      361 agaacgttat gatgaaagtg caacttatta ctactatgac tatgtgaata caaataacct
      421 ttttcctatg tcaggaatgt ttggagaaga gacctacact tatacagctc aagcgatgac
      481 tgatattgaa acattctacc ttcctgtttc ctactatgaa aaattagcac gaaaaaatcc
      541 cgaacagttg atctatatga tacaaaaaat ttctaaagta gttgaaatac atgaattgcg
      601 tatccaaatc ggattgacct ctagcgcctt tgaccgggta aaacaaaatt tattcatttt
      661 gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc accattcctt atccgatcac
      721 cttaaaagaa ttagcagtga atagcggcac gacacgtgaa actgctggac aagtaatcaa
      781 acaactacgt gattccgggc tgttagatta tgacaaaaag cgttttactt tctgtaagac
      841 agctactact ccagctatga gagaataacg cgaacaaaaa taagcagtga ccctgataaa
      901 tatcagggtc actgcttatt ttcctcccta aaacttctga aagatccggc agttttagat
      961 cactggacaa gactcaaaga ttttctatgt gtgtttcagt tatgtaatat tcactgtttg
     1021 caaaaaatac tgttagatta actagatgaa gcgtcttgtc ctcatccgat ctagattatt
     1081 cgatattcct cgacaataaa cgattactca gtgagggcaa tcagaggaac ggataatgat
     1141 aaataagctg taaaaaatag caaaataaca ataatgatta aaagtatgta aaaaataggg
     1201 ctttgacaaa aacgtgtcac agccctattt cgattaaacg gtgtgtacct gtcaactctt
     1261 cggtcacaag tcataatgtt cataaacatg aggagctgtt ttttgaaatt gttccttgtg
     1321 aatcagagtt aaacgacagg cttacgaact cttgattaaa cggcgttcaa aagcttgcat
     1381 acgcggtaat aagaacagcc aggtcaaaaa catgagaagc cgtttttcct gggctgttct
     1441 tattacttga tgcagggcag ctttttcaca acctctttat tcagataaaa ccaagcttgg
     1501 atcagcattc aactgatagc tagcaaaatt tcttttttgc atttccacat gtgctgctgc
     1561 tccaatcatt gcagcattat ctccacaaag tcgaagaggg ggaatcacta gttctacctc
     1621 agggagttta gctgccaaag catcctgcaa tccttcacgc aacccttgat tggccgctac
     1681 acctccagct ataatcagtt gcttgactgg ataattttga caagctcgaa gtgttttagt
     1741 aatcaataca tcgatcacac ttgcttgaaa gctagctgct aaatcatttt tgtctagatc
     1801 ttctccacgc tgttgtgcat tatgcaccaa attgataaat gcacttttta atccgctaaa
     1861 actgaaatca taattatctt catggatcat cgctcgcgga aaatggtaat tatcttttcc
     1921 ctgatgagct agctgatcga tttcttttcc gcttgggtaa gaaagaccta atactcttcc
     1981 aactttgtca taagcttctc ctgctgcgtc atctcttgtc tccccaatga tttcatatga
     2041 tccatcttct tgcatataga ctaattctgt atgtcctccg ctaacaagaa gtgccatcaa
     2101 tggaaactgg aatggtttaa ccagacgggc tgcatagata tgccctgcca tatggtttac
     2161 aggaataagc ggcaattggt gtgcccaagc aaaagcctta gctgcagaaa taccaatcag
     2221 caacgagccg acaagtcctg gtccataagt gacagcgact gccgataaat cttctgccga
     2281 cacaccagct tctaccagtg catcttccaa gcataaggtg atttgttcta cgtgatggcg
     2341 gctagctact tcaggtacta cgccaccaaa acgtttatga ctgtttattt gagaagcaac
     2401 gatgttagat aagatctctg ttccatttct aacgaccgct acactcgttt catcacaact
     2461 tgattcaatc gccagaatca attcttcatt catttcatcg tctccgtttt taactttctg
     2521 ctcataatga tcgcatcttc cgtcggattg cgataatatg atttccgttt acctaatata
     2581 cgaaactttt ctgattcata aagttttctt gctgcttcat tagatagacg tacttctaaa
     2641 aaaacatgca gcgaatcttt tatttgttcc gtaatcagta attgtaacaa atgccgtgca
     2701 tgcccttttc cttgttctga aactgcaaca gcaatatttg tgatctcgac ttcatctatt
     2761 actttcgagt atccaataaa gccacagatc tggttatctt cagctaaaag cagataatca
     2821 gagtgaggct gttggatgtc tgccaaaaac tgcattttcg tccaaggtga accatgtgga
     2881 taagcatttt cgctaatcat ccatacgcat tctgccaagt acgcttcatc aaaatctttt
     2941 tttctgcaaa tcaactaatc tcactcattt tctaaaagtt ttatcatatc caacgcatct
     3001 tcttttgttt cagtgtaata tctttttttg atcgttgttg cttgaaaccc caatttccga
     3061 taaagtcttt gtgcatcttg attactcata cgcacttcta atgacatcgt ctcacagcga
     3121 ttcattatag caaagttctc gatttcgtca ataagcagcg aaccgattcg tttacgctga
     3181 tatgccggtc gaacggcaat attcgtgata tggcaatcta aaccgaacgc tcttgaaccg
     3241 ataaaaccaa tcattcttcc attatcaaag atacctagat atagatgagg aagcggtgac
     3301 ttcaattctg atataaaggc actttttgtc catggcaatt cgccattata gacttctcgt
     3361 tcgacatcca gcagctcttt gatatcttct gtagtcaatt ctcttagtac gtaacgacga
     3421 tcgatttgtt cgactgtttt ttctgtataa atcattttat cttgaaaaaa gcttctggca
     3481 tacgctctaa attttttcaa cataactttc gtctccaggt gtgtgcgtct ccaaccattt
     3541 ttcttctgct tctactcgtt ttaaatagcg aggaagaaaa gcttgtttct ctgcaactgg
     3601 ttcaactaaa gcacctaatt gagccacagt tacaccactt ggaatatccc atgctggaac
     3661 tgtattaatc atcgcactag gcaatatttc ctttatttca gagaagaatt tgtgacaatc
     3721 actcccgacg aaataaatag cttcattcag cttttgtagt tgtgtgaaca aatcagaagc
     3781 agaaatatgt acatcttcaa gtacagtttc taatctattt tctttccagc ggtatgcccc
     3841 agcgtataca ttttttcttc gagcatcaaa catgggcaca ataatattat ccacaccgat
     3901 gcaattagcc gcgatggttt tcagactaga tactcctact agctctttat ctaatgtata
     3961 agcaatcgtt tttgctgtcg ttacacctat acgaagccct gtataagaac cgggaccatc
     4021 agaaaccgcg attcggtcaa tgtcttttgg acttatttga agatctgcaa acaattggtc
     4081 gattgcaggc atcagtgtta cactgtggtt gcgtttgata tttgtttgta tttgtccaag
     4141 aatttgttcg tcttctacca caccgatcgc caaagtttgg tttgcagtat ctattcctaa
     4201 tgtaatcata tatttgatcc tttctgctat gacattctcg ccttccattg tagcatactt
     4261 ttttattctg aggatgaaaa taccaaaggc aaaactagta caataataca tgattatctg
     4321 ctggcgggca aattttgccc cttcctgaaa cacaaaaaag ctgcggtatc actccatttt
     4381 cccacagctt ttccatcttt tatttcgttg attctcgaat aatcaactct gtttccacaa
     4441 taatctttct tgaaaccggt gctttggaat tacttaattg aagaatcgtc tccactgcta
     4501 gctctcccat ccattcggta taaactttta cagtagaaag cgagggcgtt acatatttcg
     4561 ctacactgac atcattgaag cccatcacag aaatatcttc tggtaccact aaaccttttt
     4621 cttgaatcgc tttcagtgcg ccaatcgcta atgcatcatt agctgcaaaa agcgcatcag
     4681 gaacatgcgg attcgttttt aaatattgct tcactgcttg atatcctgac tcgacagaaa
     4741 aatctgcttc gataattgga ccttcgtcgt agcctttttc tgccaaaact tgttgaaatg
     4801 ccataattct gggatctttt agccgctgat ggtttttctt cgtatattct gttccagcaa
     4861 gcatagcgat atgttgcgct ttatgggaga caaagtgttc aatgactttt tgaacacttg
     4921 acttaaagtc gacaactaga gagtcgattc cttgagaagt gccgtcagaa tctaccaaaa
     4981 gcaaggcatt ttgggatcct ttcatttcta ataactgagc ttcgtcgaat tttcctagtg
     5041 cgatcgttcc atctgccgga agatcactaa tggaatccca cgtttctttc aataaagtaa
     5101 cgtttgcttc ttctgccttt ttttcgattc ctaggcgaat agataaataa taaagatctt
     5161 caagctcttc cgtatcgtcg taccactgga tcaggcgaag cgtttgctta gtatttgctg
     5221 catttttacg atgtttcgta taatttaagc gttctgctgc ttcaaaaatt ttttgctttg
     5281 tggctaagcc aacggaaaga tcaggatcat agttgagaac tcttgaaaca gtagcaggag
     5341 aaactcctgc caactgcgca atgtctttaa ttgttgccat ttgtctctcc tcctttttta
     5401 cgtttataaa atcgcagtaa atcgttcaaa cgctgcctgt cctttttcat ctcgcttgaa
     5461 tactccagcg tcttccagta cacgtgcaaa aacctgacct acagattttt gtacgacatc
     5521 ggtgaccgta tctttcgtca aatcaggatg ttcttttttt aattgttctg cccacggacg
     5581 atgatattca gccatctggt tttcttctcc taataagaaa tgctctactt ctactagttc
     5641 tggtttcaaa cgaggtggta aaacagcaag ccccattact tcgatcaacc caatgttctc
     5701 ctttttgata tgttggacat ctttatgtgg atgaaaaatc ccatcaggat gttcttctga
     5761 aacattgttg tctcgaagga caaggtccaa ctcaaagtaa ttgtcccttc ttcgtgcaat
     5821 tggcgtaatc gtatggtgtg gtgtaccatc tgctgaaaaa gctctgatag atactgtttc
     5881 atccgaatat gctttccatt tttccaatac tgagttagct gcttcaataa gcaagttttt
     5941 ctctgttgat tgcaaacgga tcacagacat cggccatttc acgactcctg cgatgacttg
     6001 cggataatct tctagtttga taagtttttc tatcggtgct tttgccatag gaaattcatg
     6061 acgtcctgcc tgataatgat catgagaaag aatcgatcct ccaacaattg gtaagtcagc
     6121 attcgaacca acaaagtaat gaggcaagac ttcagtaatc cgaagcagtc tagcaaatgt
     6181 ttcctttgtg attttcatcg ggcggtgttg ttctgataaa atgatcgaat gttcattgta
     6241 ataagcataa ggggaatatt ggaatcccca gctttcaccg tctagattca ttcgaataat
     6301 ccggtgattg gtgcgggctg gatagttcaa gcgacctcta tatccttcgt tttccatgca
     6361 aagcatgcag gtaggatagt ctacttttac cgcctcgcgt tctgctgcga tttgttttgg
     6421 atctttttct ggcttagata aattgattgt gatttccagt tcaccatact ccgactctgc
     6481 cggaaatttg atgttttttg cgatggctct tgttttgatg tagtcatttt tcttacttaa
     6541 attaaagaaa tactcagttg cttcctctgg gcttttcgaa tagtgctgtg caaagaatgc
     6601 attgacgact gaaggtggcg gtgtgagaaa atccattaat tgcgcttcaa aaatctcttg
     6661 ttctgctggc aattctgaaa gaacaccatt ttctcttgcc ttagcgacaa gctgatccaa
     6721 taagtctaca gattcttcct taactggtct tacttcaatg ctttctagag catcttctcc
     6781 taccatccct aaaagacgat tctgtaaata taagcggtcc atttccatcc atccgccagc
     6841 ttcgatcgca agtgtggcaa aatcacaaat cgtttgactg attgacatat ctcattcccc
     6901 tattcttcat aaccatttgg atggctgaca tgccaatccc atgctgtttt gataatcgct
     6961 ttgatatctg tgtattcagg tttccagccc agaatctctc tcgctttttc acttgaagcg
     7021 actaatcggc taggatctcc agctcttctt ggtgctattt ctgctggaat ttcttttcct
     7081 gtcacttctc tagctgcttc tagcatttcc ttgactgagt atcctttgct gctgcctaaa
     7141 ttgaagaagt tgctttcatt tccttctttc aagtattcca aagctaaaat atgtgcagca
     7201 atcaaatctt ctacttgaac ataatctctg atacatgttc catctggtgt atcataatcg
     7261 tctccataaa cagccaaggc ttttctttga ccaagagcta cttgtaaaat gattgggaca
     7321 agatgcgttt ctggtgtatg atcttctccg atagatgcat ctgcttttgc tccggctaca
     7381 ttgaagtagc gcaaagcgac atagcgcatg ccatatgctt ggtcacacca cttcatcatt
     7441 ttttccatca tcaatttact ttcaccatac ggatttttag gattagctgg tgtatcttct
     7501 gtgattggag actctttcgg ctctccataa gttgctgcag tagaagagaa tacgatttta
     7561 ttcacgttga actcgtgcat gacttcaagt aagatttgca taccgtatac gttgttgttg
     7621 aaatacatca atggtttttc gacagattct cctactaaag agcttgcagc aaaatggatt
     7681 actccttcaa tcggttcctt ttcaaatacg gaacgtagaa attctttatc ccggatgtct
     7741 ccttcataaa aatgagcatc tggatgtact gcttgtttgt gacctgtgag caaattatct
     7801 accacagcta cttgataccc cttttgaact agctggtcta cagcgtgcga accgatataa
     7861 ccggctccgc ctagtactaa aattgtcata aaaaacactc ctttgattag taaaattgtg
     7921 tttgttgatt ctatataatt taaaaaacga agtttacttt tatggtgaaa acaaacaaag
     7981 taaaatttcg taaattcata tggtaaattt ataataaaac atttagtaaa cattttcaag
     8041 ggttttttgc aaaggtttac aaacaaacgt aaaactagcc tttttttctc ttttgttcca
     8101 taatagatat aacaaataat aaatgaggtg gattcaatga aaaaatttgt atctggaata
     8161 ttagtaggaa gtctggcaac agccgctgcc gttgctggtt tggttgcttc tgtgaaaaaa
     8221 acagtgatcg atccaattga cgaaaaagaa gcaatgatcg aagaaaaccg taaaaaagca
     8281 atgcgtaaac gcgtttctcg ttaataatta tttaaaaata cgcatgaaga ctttctaaaa
     8341 aacagataag tcatcatgcg tatttttatt tgtcagtcaa tataagttgt gctattttat
     8401 ctgcctgtct cagattccat ggatctgtat aaggtctagc tactgctgca gttagccaat
     8461 ccacaccttc aacaggtatt ccatagcaat aaaagtcaga ctggatttcc cctgaagcat
     8521 caatcacgcg attcgtacct cgctcaacat ctatagcttc tgaatgataa cttcctgtat
     8581 tatctgaaaa tgaaaaaggg cgaacaagtt tgttttcctt caaatttttt atgacaggat
     8641 tacttgaacg agaaaagctt gtttgtggca atcgagcttc aatcaaatag cgactgctga
     8701 tttctttatc ttgtagagaa ataacatagc ggttgtgctt ttgctgaata tcaaatggcg
     8761 gttcgatcaa atgaaaaata cccgcatcta tcagtgctgc taattctttc gtccgaatga
     8821 ttggcggacc gatcgttaaa aagctattta gatgagtaaa ccatccccat agtatttctt
     8881 tgtattcctt agcagaaaaa acgtgccatt caatagcctg ccggattgga tcacgccagt
     8941 cttttaatgc atcaaatgtt gaagcaaatg ctcctgtgca attgcctttt tccgcttctt
     9001 ttatctgccc ttcaataaat attcggctag cttctgaaaa gggctcattc tgataggcaa
     9061 gaggagtttc taaatacgac caatcccatc tataagggat tagtgctgga tgctttccga
     9121 gacactcctc tgaagagagg gagagaaaat catgctcaaa atttttcttt ggaatcggca
     9181 agtgatattc ttcgatgatt ttcttgtaat aaaacaactc aacgtctttt ttcagatagt
     9241 caaaaaatgt ttcgccagtc aaaaatcccg actgatgcca ttgctccagg ttttcttttg
     9301 ttagtaaccg tggccatgcc attgctccgc cttgcttttg atttcttcct cgagggtaat
     9361 aagggagccc ccttcttgat ccactgtata caatcggttc cttgcctgat ggatgataga
     9421 ccagttttcc cttttcgttc tcgaagcgcc ctcctcttga tactgtaaac aatccgacat
     9481 aatcaaaaaa acttaagcct agacctctta aaaagacatt ttctttaggc ggtatcttcg
     9541 aaagatctac atctgcagga ttagaaggaa gctgatacag taaaacattt ttctttgcgt
     9601 agtctaccca cactctttct tcttctgtct catcattttc ccagtgaccg cttgctaaaa
     9661 caagctgatc gatagaatag ctttgctgat ctgtctgtaa aagataacca tcttttcttt
     9721 tgcttacttt ttttactaaa gaataaacaa actgaataga agctggaaat tcttcttgca
     9781 actttgagaa aaaccagcgt tgatagagtc cgtataaaca tcgagtagat tgctgatctt
     9841 tttccagacg attgatttct tctataatta gttgcttatt tttggttacc gtctgttcga
     9901 tataatctgt agcaggtcct ttactccatt gaaacaagtt gggtccctgt gttaccaatc
     9961 ctccactgga caatgtttca tctgtaaaaa gcgtcacttg ttgagaaaca gaattcatta
    10021 ataactccgg tggctgattc aaccgccaaa ctcgacctcc aggaccattg ggatcgaaaa
    10081 gaaaaagttc aagagaatgt ctgtcctgat tattatggag aatcctttct gctacactta
    10141 atcctctagg tccggcacca atgatcccta ttttcatttg catgcctcca tttcgataaa
    10201 ttttttcaat aaaacagcat ggttctgatg ttcatttttt gcaccataaa caaaagtaat
    10261 gactggatgt tctttcataa gttgactgag tttaaaaata gcttgcttct tttcttcttc
    10321 gaaaatcaat tcttgctggt atttctttgc aaatgaagca aatcgttctg gtacatggtc
    10381 aaaagttttg cgtgcttcat tactaggtgc aatttctttc aaccacagat ctaactgcgc
    10441 tttttctttt gaaattcctc gtggccagaa tctatccacg agtattctat agccatcctc
    10501 tttttcagca tcaaggtaaa cacgttttaa tttgatcata gttgtttcct tctttctacc
    10561 ttttcgctta gcttatatat gtagataata gcacaaaata aaaaaaatga acggcacaac
    10621 tatccattgc gccgttcatg tgatcttggt caaaatctaa tgattgattc ggtttttaaa
    10681 gctgtcatac aatcatctgt tttatacttt tttacttttg atttttccag accatccgtc
    10741 atatccaccc ttaagtaaat aaatctcttg ataacctgct ttacgtaatt tatttgctgt
    10801 acggatgctc aaacttttct tttgatcgta aatatagaca ggctggtctt ttcgaataga
    10861 tcctaatgaa tctttcaaaa cagtgtaagg gatgtttctt gcacctaaaa tatgcccagc
    10921 atcaaacaca tctttttcgc gaacatcgat tacttgtgct tttctcattc cttctcgaaa
    10981 ttcttcttct gtcaaagtcg ttgctgaacg tttggccatt actcgtagat agacttccca
    11041 aaggatgatt gcaagcacaa tcaatagcaa aatgatatta attacccata aaaccatgta
    11101 atgcctccgt ttttttaagc aaatttcaag gctaatgagg cagcaccaat gacaccagct
    11161 tcattaccaa gctctgctaa tttgattttt gttgattctc ttacttgcgg gaaagtaaat
    11221 tctttaaaat atttctctac acggctacgt aaaaattctc ctgctgccga tactcctcca
    11281 cctaacacga tacttgacgg atttaatgta ttccccaagt ttccgcaagc taaacctaag
    11341 tagaagcata cgcgatcaac aaccatcaat gcaaacggat cgtcttcttg agccatttca
    11401 aagatatctt tacttgttac ttcttctccg ttgtctagca ttgctttcag ttttgaatcc
    11461 cctgcgtatt cttctgccaa gaaacgacct aggcgtacca cacctgtagc gctagataca
    11521 gtttctaaac atccgcgttt accgcaagta cattcgaacc cttcaggatc cacagtgata
    11581 tgtccgattt ctcctccagc tccgctcacg ccgtgaatca aatttccgcc agcaacgata
    11641 cctccgccaa cacctgttcc tagagtaacg aatacaacgt ctggatcgtt ttctccagca
    11701 cctttccatc gttcacctaa agcagcaaca ttcgcgtcat tatcgatagc gaatttgatt
    11761 cctgttcctg cctcgatcag ttttttcaca aattgaactt ctgtccagtt taagttatac
    11821 gcaccaataa ccgttccagc ttcgctgtcc acgcttcctg gggtacccat gccgattccg
    11881 ataaagtctt cggctttcat attatataaa ttcaaacgat gattgattga ttcaatgatt
    11941 tcaggaacga tatgcttacc atcatccaaa atattggtat cgatgctcca tttttgctga
    12001 atctctcctt ctggtgttaa aattgcaaat tttgctgttg ttccacctaa atcaatccca
    12061 ataatcttct tgtccattac aaatttcctt tctttcggct ttcttcaatt cggtgctctc
    12121 tttttaatat gcgccacgct gtcatgtatg tatcatgatc aatcaaacgg ctatcataca
    12181 gattacgcaa ttcgatagtc attaattcaa tgtcatagat gcgtgcaccc acatagataa
    12241 agatgccaaa ttttttgaac agctgctgta catcatataa gctctccatc tttaagcact
    12301 tccttcatat tatacaagta aaccatattt ttttaaaccc agtaaaatac atattacaaa
    12361 aagaaataca aaaactagtg tagaaagaat cctttggtgt atcgaatagc ttgtctgttg
    12421 tttcggtaga cccaaaacat tccctaacac taatccgcca attagcccac cgacatgtcc
    12481 ccaaacatct acagaggtat ccagaagacc aaaaacaaat gtcaacacaa taaataaaag
    12541 aaaatgtctg actagctgct ggatcgctgg gttatgttta aaatgaaacc ctaagataaa
    12601 caacgcaccg aacaaaccga atatcgaagt acttgcacca ccagacagaa cattcgcttc
    12661 gttaaaagca aagcttgctg tatttcccat tatcccgctg aacatataaa tcaagaaaaa
    12721 acgccaatgg ccatagattg cctcgatttg ctgccccata aaatacagta tcagtgagtt
    12781 cattacgaaa tgcatcagtc caaaatggat gaagattggt gtcactaaac gccaccattc
    12841 attgaagtga acaagataag gaccatacat tccggcataa taagggatct caagtcgcgg
    12901 aaataattcc atcatgagga agaccgctgt ctgtatccct aaaaaagcat aggtccataa
    12961 cggtcgtctt aaccatgcac gcaattttat ttgctgttga taattcatga aaacctccca
    13021 tttcatttta gtcaataaac aattgttgga tcttctgatc aaagctctcc ggttgccatt
    13081 gttcattcaa ttgttcacga aaaacaaggc tacatgtttt tttatgaaaa ttatttaaat
    13141 accggtcgta ataaccaccg ccaaaaccga ttcgataacc ggctctattg tagataatac
    13201 ctggtacaat gaggagatcg atcatctccg gcaaatagag cttctctgat tgtggctcaa
    13261 gaacaccaaa actcgaacgt acaagaacgg actgttcatc catttcataa aacgccatct
    13321 gacgtttcgg caaagtttta ggtacaacta ttttcttttg ttctttttct gctcgttgga
    13381 taaccggctg tgtatccagt tcaaattcat tcgataaagt cgtccctatc acttttgctg
    13441 acttccagta ctcgctggcg aatagctgtt tatagattcg ctgttctttt tcctttttct
    13501 tgccactttt ggccagattt ttcagctttt cgattccttt ccggcgtaat cttactttca
    13561 attggtccaa aaactcaccc ttccttctct cataaaatgg tataggaaaa aaagaaaaat
    13621 agcaatcgaa ctttcaaata aatgcataaa tagaataaaa agaagaccta gaaatgattg
    13681 gccttccttt tttaatgaat ttcaactgtt cagcgcgtgt ttttgtctga taaaaagtgc
    13741 atacagaaaa tatggcgcac caaaaagggc aatcaaaatt cctgtaggta cttccccact
    13801 tggtagaatc actctagcaa ccatatctgc actaagcaga aaaatggaac cgaacaaacc
    13861 gctcagaaca aaggtccagt tattttcttt acgtacgact agcttcgcag catgaggagc
    13921 tagtagtcct acaaaagata aactacctgt caccgctaca ctaccgcttg ccaaagcaac
    13981 agctaacaac aaaaggattt tttgactttt agccacgtta aggcctaatc cttgcgcgcg
    14041 ttcttgacca aatgatagca atacaatcct ctgtgcataa agtcctccta gcggaattaa
    14101 gataaccgcc catggaagca atgcccaaac ataaggccag ctcgcacccc agattgatcc
    14161 cgctaaccat gcattcacaa atccataatt ttccttagat aattggatcg tgcatagcaa
    14221 tgttaatgca gaaaggccgg cattaacggc aataccggac aataagattc gattcatacc
    14281 caaaaagcca tttcgatcat aggccgcaag atagacacag caagcagcta aaacaccacc
    14341 tagaaaggct acaactagca atagaaacgg cagcgaatag tgcgaaaaga aacctaaata
    14401 aagcatcact aaaaatcctg ctccggcatt aatgcccaat atactagaat ctgccaattc
    14461 atttcttgtt actccttgca agatataacc agaaacgcca agacaagctc ctgctaataa
    14521 actgatcagt aatcgaggaa ctcgaaattc tctaacaatc agcgccaaag aagatgattc
    14581 tttccctatg aacgtggcaa aaagctgatc caggtaaata aaaggcgtac caatcattaa
    14641 actacctata gacaggatga caagtacaaa aagcaagaaa tagaacgtcc aatatatttt
    14701 tttcaccgtt gttccctcct tacttgtatc agtaaaaacg gaacgccaat caatgcaatg
    14761 actaatccaa aaggtgtttc aaaaggggga ttgatcatcc tcgctgtaaa atccgccaac
    14821 ataaccaatc caccgccaac taaaaaagag acaggtagaa cataacgata gtccgttcca
    14881 accattccgc gaacaactgg cggaaccaac agcccaataa atgaaacagg tccgactaac
    14941 gaaacggcaa tggctgctaa cactgcaact agacagaaaa caatcaaccg cgttcgttta
    15001 ggatgacttc caagagctaa tgcatgtgtt tcactcaaat gaatcaaaga tattgctttt
    15061 cctaatgaaa aagaaaccaa aacagcaagt atatataaag gaaacaatac tgctaattgt
    15121 ttccatgtga tattggcagc tccgcctaca aaccaaaaag caagatcctg ctgcagatta
    15181 aagaattggg taagtgcttg gctgatagca gtaaaaaaag aactaatagc tacgccagct
    15241 aaaatcagct gtaaaggatt catgcccatt ttcgaaaact gcaaaaaacc aaatacgagc
    15301 aaagttacag caaaagaacc tgccaatgac attagaaaca cagaaaaatc agaagcttta
    15361 ggccaaaaag caaaactaag ggccattgcc aacgaagcac ccgcgttgat tcctagtaag
    15421 cctgaatctg ctaaaggatt tcttgtcact ccttgcatca atgcaccgct caatgcaaaa
    15481 caactcccta caagaaatgc tcccgccaca cgaggcaaac gaatatttcg tatgatttgg
    15541 tgactttgtt ttgatctatc aaaatcaaac aacgcctgcc aaaaatctgc tttttccaca
    15601 gtcgctgcgc catttgaaag agcaacaaaa aaaataatca aaagaacaaa acagaccaaa
    15661 agcaggagtc tatttttctt catagtcctc ataattgatc ctcctgctct tttggcaaca
    15721 aatcataggt tagaaaaact ggtttatttg ttcttggaag cgtgacaatc gttgcatcaa
    15781 tctcaaatat ttttttcata cactcttttg tgatcacttc ttcaggtgta ccttctgtga
    15841 tcaacttacc gtttttcatt gctaataaat aatctgaaaa acgtgaagca tgattaatgt
    15901 catgaatcga catcaagatc gttgtttgtt tcatttgatt gatttcagtc aataactgta
    15961 ggatctctaa ttggtgtgcc ggatctaaaa atgttgtcgg ttcatccaag atcaagatat
    16021 ctgtttcctg agcaagcgcc atagcgatcc agacacgctg ggtttgtcca ccagacagcg
    16081 ttgcgatatc ccgcatacga aatccagcta gacctgtagc atccaacgcc cactcaattg
    16141 cttcatgatc ttgcgtgtcc atgccactga acagtttttg ataagggtat cgtccgtaag
    16201 atactacttc ttctactgtc atacctgtag ggtgttcact agtttgtgct aataatgcaa
    16261 ttttttttgc aagttctttt gaactgtatg tagagatatc agagttattg acaatgatct
    16321 ttcctgattt cacaggtagt atacgggtaa aacttttcaa taaagtagat tttccacttc
    16381 catttggccc aatcaaacta gtgattttcc cttcaggaat cgtaacagac atgttttcaa
    16441 tgattttttt gttttcgtag ccggctgcaa tgtcagctgc tcttaatctc gtcatttcac
    16501 ccatcctttc gctgaaaatc attctcaatt atgacgctgt ttcaacattt cgtcaagcgc
    16561 tactttcata tctatagcca aatgaagcgg ttgacagcgt tcgaaatatc aggaataata
    16621 accaatgaga aagattctca attatgttag gaggaaagaa atgaaattaa aaacaaaagt
    16681 attcacatta tctagcatat tgctgctggc tggagtactg agcgcttgct ccccctcttc
    16741 agataaagaa acagctacaa ctgccaattc gacgacttca tccaatagta ctcatacagt
    16801 cacagataca cttggacata aagtagagat tccaaacgcg cccaaacgga tcatcggcag
    16861 ttacttagaa gactatctga ttgcactagg ggaaaaacct atcgcccagt ggactgttgg
    16921 cagtggttcc attcaagatt atctgcaaga tgatttaaag gatgtcccaa cgatttctta
    16981 tgaccttcct tatgagaaag tgttaagctt tgagcctgat ttgctgttga tttcttcttc
    17041 cgctactgtc gaaggcggga aatacgaaca atatagcaaa atcgcgccaa cttatgttgt
    17101 aaaaaacgga acagatgtca catgggaaga acaactaaaa gatgtcggca aagctttggc
    17161 aaaagaaaaa gaggcagaaa aaatcatttc cgattatcaa aaatccatca agaaaactcg
    17221 agaagaacta gcagataaaa tcgagaacaa gacagctgca gtgttgtggg tgaccaacaa
    17281 ctcagccttt atggtcagca aaaatcggtc aagtggaaga atcgtatacg acgatctgga
    17341 atttggtgtt cctgatctag tggaagaggt ctccaaaaaa gccacttctg actggtctgc
    17401 tgtttcctct gaaaagctag ctgaattaga tgctgactac atcatccttg tgaacagtga
    17461 caaagatgct gccatgttca atgaaccaag ctggaagaat ctaaaagcag tgacagacaa
    17521 tcatgtactg caattcggac ctgaatctag ttggctatat aacggaccta ttgccagtca
    17581 ggaaatggtc aatgatatca aagaaaaact aaattgattt taactaaaaa aagcatcttc
    17641 acgattagtc gtgagatgct ttttttctta ttaacaaact agacgcatag attttattct
    17701 tcttttgaag caaccctatt ttttactctt tcccattttt gaaaacgatg ggcatataga
    17761 ttttcttgat ccgttatccc ctcatactcc tctataaggg cgaagtctga aaaatcaata
    17821 ttcccaatat acgtatcgcc ttcaaacgtg ctatcgatca atgttcgcca gatccgacca
    17881 gtgtaaggaa gaagttcttg aaaaatccga ctaccacccg aaacatagat atcttctgag
    17941 atcgtttgac tatacgacaa aagttcatcg atcgaatgca ttacttccgc attttctttc
    18001 gtgcgaaaat ttggttgcgt tgtcaacacg atgatatggc gataaggaag tgaaagatcc
    18061 cccattcctt cataggtctt tcttcccatc actaaaattc gatccattgt atgttcgcga
    18121 aagaaacgca tatcatttgg caatcgccat ggtaacaaac catttttgcc gatcagacca
    18181 tttttatctt gtgcccacat ggaaataaac atattatccg ctccttatga ttttagatgt
    18241 tttgagataa aaaaagtctc aattctttga ccagcaagga attgagaact ttaaaaacat
    18301 tattttgttt cacggtgcaa agtaactttg cgttcgcgtg ggcaatattt ttgtttttcc
    18361 aaacgatcag gattgttacg tttgtttttg cttgttagat agttacgttc tttacaagaa
    18421 gtacactcta aagtgatgtt tacgcgcatg tttttccctc ccatttttac aagaatcctc
    18481 taagatgact ctcagacttg attatcttac catgattttt tctagattgc tagtgttttt
    18541 cacgattttg ttaagtaaaa ctacttgacg ataaatacaa cggttttact tctcaaacaa
    18601 aatctttttc tttttgtata aataccctta cacttgttta tatagattat gttttttaat
    18661 aaagggggat actaatatgc catggaacat gaaggacttt cctgcatcaa tgaaaaactt
    18721 agataaattg acaagaaaaa aagcaatcga catcgcaaat gcattattag atgaagggta
    18781 ccctgataac cgtgcgatcc caattgccat tgatcaagca aaaaaatggt atgaagatgc
    18841 tagtgaatca gaaaaacgaa ctttcgaaaa agaaaaaaat ccaagcaaga cggatgaaca
    18901 cgatacgaat cctcgtgctg gaaaactgct tgattcagat gtgatcgtag aatatgagga
    18961 agaacaatgg atcgtgaaat ctaaaggggc aaaaaaggca agcaaccatt ttgatacaaa
    19021 aaaagaagca atcgaaaaag gaaagcaagt agcacaaaac aaagaatcaa ctttgttcat
    19081 ttataaaaaa gatgggacaa aagaaaaaga aatcgattat tgaacatatg taaaggggtt
    19141 gtgacgaaaa tcgtgtcaca acccccttat gttcgaaaca atggtgttcc tctgccaact
    19201 ccgaaccata agtcataata tctataaaac gggaagagct gttttttgaa attgtttctt
    19261 atcactcagt gttaaacggt aggagaaaac ctctttttta ttgatatttg attttcatca
    19321 aacagtcaca tagtcgattg cttctgcttg cggaatcgtt tcatctacaa agtagacttt
    19381 ataccatgtc aagaaactga agattgtcca gatttttctt tgctcttctt tacgtccttc
    19441 gtgatggtca tctagcaact tcatgatttt ttcttgatca aaaaattctt ttgcaaaatc
    19501 tgcttcgaat aatgctttga cttgttcgta gccgtgttct tctttcaacc aagctttgat
    19561 tggcacaggg aatcctaatt tcacacgatt cgaccattct tccggaagat gtttgttcgc
    19621 agcttgtctg aaaatatctt ttgtgttgtt ttgattcaat aaaaacttac ttggtatttg
    19681 ttgagccaat ttcatgactt cgatatccaa taaaggtact cggacttcca aagaacttgc
    19741 catagaaagt ttatctgcct ttaacaagat atcttttggc atccattgat gaagatcgac
    19801 atattgcatc ttattgactt catctttgat tccttctgtt ttctcgtaat gaacagtcat
    19861 gatttcatcc acagatggtg ctgtttgata ttttggtgtt aacacttctg atgcttctga
    19921 ttcttcgaac actcgagcat gaccgataaa atattctttt gcaggagcaa gtgattcata
    19981 taaatggatc cgtccatgga agtttttcat ttttccgatt ttacgtccta aattatagcg
    20041 tgttcctttt ggtaattttt tcaatccttg agcaatcaca cggatgaatt ttgaattcgt
    20101 atggaatcca tacgtttggt atcccgcaaa cagttcatcc gccccttctc cagattggac
    20161 tacccgcaca ctttgtgaag caagatttgc taaaaagtac aaaggtacgc aagaaggatt
    20221 tgaatcaggt tcatctaagt gatactggat caaagggaat gctttgaacg acatgtctcc
    20281 gtcgatcact gctgctgtat tatctaagtc caatagttcg gttagttttc tggcttcaat
    20341 cgcttcatta taggttttat catcaaaccc gattgagaaa gaatgatctg gtttcaatac
    20401 agaggttaca taactggaat caaccccgct ggataagaaa gaaccaactt ctacatcact
    20461 gaccgtatgt gcttcaatag aagcttgaac ggtttcgtcg attttttcga tccattcttg
    20521 tcgggaatgc gtttcttttg tttcaaaatc tgcatcccaa tattgctgaa tgtctatttt
    20581 tccatcttta tacgtataat aatggccttc aggcaaacga tagacatctt tgaagaatgt
    20641 ttcgccgttt aatggagaat attggaaagt catataaggt ttcaacgctt ctttgttcaa
    20701 ttctttgtta aaatcaggat gaggcagaaa acttttgatt tctgaaccaa acataaatgt
    20761 tccgttcatt tctgcataat aatatggttt gatcccaaag tggtctcttg caccgaataa
    20821 ctcattcttt tcattatccc aaatagcaaa agcaaacatt ccgcggatct tttgcaataa
    20881 ttctgttccc cattcttcgt aaccatgcaa aaggacttct gtatctgcat gtgtttgaaa
    20941 aacgtggcca gctgcaatca attcttctct caaaggctgg taattataga tttcgccatt
    21001 aaaaataatg atctttgtcc gatcttcatt gtaaataggc tgagtaccgc cctctaagtc
    21061 aataatactt aatcttctga atcctaaagc aacatgttta tcgatatatt ctcctgaact
    21121 attaggtcca cggtgaacga ttcgatccat cattgtatta ataattgttt ttttgttgtc
    21181 cttgtcgtta acaaaaccta caataccgca catttaactt tcacatccta atataaaatt
    21241 actgtttatt tcattcatat gcatcttatc atagaataaa tagcggacga atattttctc
    21301 tcttacaatt tcgtcacgtg aaaacaaaag atgaaagttt ctgtttttac ag
//
LOCUS       DAYYKN010000043        20896 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000043 DAYYKN010000000
VERSION     DAYYKN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.043"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..766
                     /gene="rnc"
                     /locus_tag="ACHBB7_001303"
     CDS             80..766
                     /gene="rnc"
                     /locus_tag="ACHBB7_001303"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM9209263.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            786..4367
                     /gene="smc"
                     /locus_tag="ACHBB7_001304"
     CDS             786..4367
                     /gene="smc"
                     /locus_tag="ACHBB7_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM9209264.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            4376..5185
                     /locus_tag="ACHBB7_001305"
     CDS             4376..5185
                     /locus_tag="ACHBB7_001305"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9209265.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            5196..6194
                     /gene="ftsY"
                     /locus_tag="ACHBB7_001306"
     CDS             5196..6194
                     /gene="ftsY"
                     /locus_tag="ACHBB7_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM9209266.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            6381..8648
                     /gene="gshAB"
                     /locus_tag="ACHBB7_001307"
     CDS             6381..8648
                     /gene="gshAB"
                     /locus_tag="ACHBB7_001307"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM9209267.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(8737..9189)
                     /locus_tag="ACHBB7_001308"
     CDS             complement(8737..9189)
                     /locus_tag="ACHBB7_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM9209268.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            9339..10289
                     /locus_tag="ACHBB7_001309"
     CDS             9339..10289
                     /locus_tag="ACHBB7_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9209269.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            10282..11241
                     /locus_tag="ACHBB7_001310"
     CDS             10282..11241
                     /locus_tag="ACHBB7_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9209270.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            11238..11993
                     /locus_tag="ACHBB7_001311"
     CDS             11238..11993
                     /locus_tag="ACHBB7_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209271.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            12016..12969
                     /locus_tag="ACHBB7_001312"
     CDS             12016..12969
                     /locus_tag="ACHBB7_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9209272.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(13613..13909)
                     /locus_tag="ACHBB7_001313"
     CDS             complement(13613..13909)
                     /locus_tag="ACHBB7_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM9209273.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            14264..14620
                     /locus_tag="ACHBB7_001314"
     CDS             14264..14620
                     /locus_tag="ACHBB7_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9209274.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            14630..15529
                     /locus_tag="ACHBB7_001315"
     CDS             14630..15529
                     /locus_tag="ACHBB7_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9209275.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            16049..16777
                     /locus_tag="ACHBB7_001316"
     CDS             16049..16777
                     /locus_tag="ACHBB7_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9209276.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            16922..18949
                     /locus_tag="ACHBB7_001317"
     CDS             16922..18949
                     /locus_tag="ACHBB7_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM9209277.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            19057..20154
                     /locus_tag="ACHBB7_001318"
     CDS             19057..20154
                     /locus_tag="ACHBB7_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM9209278.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            20461..>20896
                     /locus_tag="ACHBB7_001319"
     CDS             20461..>20896
                     /locus_tag="ACHBB7_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9209279.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT         7085 a         3543 c         4387 g         5881 t
ORIGIN      
        1 accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc
       61 cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa
      121 catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa
      181 tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt
      241 tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa
      301 attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga
      361 atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac
      421 gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca
      481 gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg
      541 tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg
      601 tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt
      661 ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggga aatcgaaaaa
      721 attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga
      781 aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag
      841 tcattgattt tgaaaatagc gtcacagcag tggttggacc aaatggcagc ggaaaaagca
      901 atattacaga ggctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg
      961 gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag
     1021 aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac aatgagatca
     1081 gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct
     1141 gcagattaaa agatatccaa gaattgttct tagattctgg attaggaaaa gaatcgttct
     1201 caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg
     1261 ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac
     1321 agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg
     1381 aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa
     1441 aagaaacact gactcaaacc gatgtctctt tgatggttgc tgaaatcaag acagcaaaaa
     1501 aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat
     1561 ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag
     1621 ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc
     1681 agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc
     1741 aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc
     1801 aagaaagctt gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa gctgaagcaa
     1861 atctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattattgg
     1921 cagaattaag agatcaatat gttgatttga tgcaagaaca agcagctgta ggaaacgaac
     1981 tgaagtatct agaacgtcaa tatatacaag aaacagcaaa aagcaagcag acgttagcca
     2041 agcaatcgga agtagaagca tcagtagacc gattgatctt acaaaaagaa gaattgacac
     2101 aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc
     2161 aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat
     2221 taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa
     2281 actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg
     2341 gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg
     2401 aagcagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc
     2461 aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga
     2521 caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt
     2581 ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa
     2641 acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa
     2701 cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat
     2761 ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag
     2821 aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa
     2881 aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc
     2941 gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctacaaaaca
     3001 tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg
     3061 aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa
     3121 cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa
     3181 gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag
     3241 atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag
     3301 tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga
     3361 cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat
     3421 tatctgctca gcgagaaaca ttaaaagccg agttggcaaa agccaaagag cagcgagata
     3481 gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa
     3541 aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt
     3601 tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc
     3661 aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag
     3721 aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca
     3781 gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat
     3841 tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg
     3901 aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg
     3961 aattagtact gacggaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac
     4021 ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg aggagaacga gcacttacag
     4081 ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt tccgttttgt atcttagatg
     4141 aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt
     4201 tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag
     4261 atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga
     4321 tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat
     4381 caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga
     4441 tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac
     4501 aggacgcccc ttagctgcga tggcgcatta tctgcaagaa ttaggactgg tagatgaagg
     4561 tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat
     4621 ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt
     4681 ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag
     4741 gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag
     4801 cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt
     4861 agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac
     4921 aagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt
     4981 attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg atgaggaaaa
     5041 tgatttaccg atgatccaat atgctggact aggcgtagcg atggagaatg ctgtgccttt
     5101 cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt
     5161 gatccaaaaa tttgtattag agtaggggga gtcaaatggg attttttgat aaaatcaagc
     5221 gtgcttttac tggcgaagag aaaacagaag aagttgaagt acaggagaag tacgacaagg
     5281 gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa tgagttgttt gccaattttc
     5341 gaacagtcga tgaagacttt tttgaagaat tagaagaaac acttatcggt gcagatgtag
     5401 gttttgaaac agcgatacga atcaccgaag cattacgtca agaagtgaaa ctcagaaatg
     5461 cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa acttgtggat ctctatgaag
     5521 cggaaggaat caatgaagta aatgaactaa atatccaaga agacggacta acagttattt
     5581 tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat tggtaaactt gcccatcagt
     5641 tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga taccttccgc gctggagcaa
     5701 tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt ggcaatgcag
     5761 gaggcgatcc agcagctgtc gtattcgatg ctttggaaag agcaaagaca gagcaggcag
     5821 atgttttact agttgatact gcaggccgac tgcaaaataa agtgaacttg atgaacgaat
     5881 tagaaaaaat aaaacgagta atccagcggg aattaccaga agcaccccaa gaaacacttc
     5941 ttgtattaga tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa ttcaaagaaa
     6001 caacagatgt gaccggtctt gttttgacaa aattagatgg tacagcaaag ggcggtatcg
     6061 tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt ggtgaaggaa
     6121 tcgatgactt ggagccgttt gatccaaatg attttgtcgt tggattgttc aaaggattac
     6181 taaaagaaga ataacccatg tctaaaaagc aggagctgtg acaaaagttg cagctccttg
     6241 tttgaatgat tgtttttaac atagctgtgt ttttgatgcc ttcattttta tacttatttg
     6301 taagcggttc tcaaacaatt tcgttctttt tcttagactt tttgattata attagattca
     6361 tatgaattgg aggtcactta atgatgaatt ttaagcaatt attattgcat gtcaatgcgc
     6421 gcccttttat cgaccaagct cgatttggta tagaacggga aggacagcgg gttgatcttg
     6481 caggaaatct agcaaaaacc gatcatccag caatctttgg cgatcgatcc tatcacccct
     6541 atatccaaac agattttagc gaaacacaaa cagagatgat cacccctgtt accgattcta
     6601 ttcccgaatt atttcagtat ctcgctgctg tttatgatgt gactgctcgt tctataccga
     6661 aagaagagat gatctggcca ttaagtatgc cacctgcctt accggaaaaa gacgaagaga
     6721 tcattattgc aaaattaaaa aatttcgaag atgtcttgta tcgacgttat ttagcaaaaa
     6781 aatacgggaa gcgaaaacag atggtaagcg gtattcattt caattttgaa tttggcgatg
     6841 agttgctaag aacattgttc agccatcagg aagaatttca agatttttcc gaattcaaaa
     6901 cagaacttta tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg atcacttatt
     6961 tattcggtgc ttcaccaatg agtgagaaga actacttttt agatgaatca catccgcaag
     7021 aacctgttcg cagtattcgc aacagtgcgc tgggctatac gaatcatcca aatgtgaaag
     7081 tttcctatgc ttctatgaaa cagtatttag cagatatcga gcgaatgatc gaagaaggga
     7141 aactttcgga ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga aagaaagtcg
     7201 cagatttagc aacaacaggt gttcgttata ttgaattgcg gaatatcgat ttgaatcctt
     7261 atgcaagatt gggtatcaat ccagagcaag ttcggttctt acaattattc ctgatgtaca
     7321 tgttatggac agaagaaaaa gaagactgtg accaatgggt agcagaaggt acgactcgaa
     7381 ataacaaagt agcactagaa caaccatcag atcaaacaga atttcatcaa gaaggtagag
     7441 aaattcttga aggaatgaag caaatgctgg ttgaattgga ttggttggat tctctttatt
     7501 tagtagagga agcattgact caaatggatc atcctgaaca aacgttagca gcgaaactct
     7561 atcaagaagc acagctatcg agtcagcaag aagttgctgt ggcattggga catcaatatt
     7621 ataaagaaag ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg gaactttcta
     7681 ctcagatatt tatgttcgat gccatccaaa aaggtgtcca agtcaaagta ttagatgagt
     7741 cggatcaatt tttgcgtctg caattccaag accatgtaga atatgtgaaa aatgcaaaca
     7801 tgacaagtaa ggacagctat atcgtcccac ttatcatgga aaacaaaaca gtaacgaaaa
     7861 aagtcttaaa agaagctggg ttccgggtac caggaggcgc agaattttca tccatggaag
     7921 aagcggtaaa agcttatccg agatttgccg agcaggcatt tgtgatcaaa cctaaatcaa
     7981 ccaattacgg gttaggtatc acgattttca aggaaggagc tagtctggaa gattatcaag
     8041 caggtttagc cattgctttt cgtgaagaca gttcggtttt agtagaagaa tttatgccgg
     8101 gaacggaata tcgtttcttt gtgatcgacg gagaagtcca agccatcatg ttgcgagtcc
     8161 ctgctaatgt tattggtgac agtatccgaa cagtgaaaga acttgttgaa gaaaaaaata
     8221 gtgacccttt gcgtggaacg aatcatcgtg cacctttaga attgatccaa ttaggtgaat
     8281 tagaacaatt gatgttgaaa gaacaagggt tgacgatcga atcagttccc caagccaatc
     8341 aaattgttta tctaagggag aactcaaata tcagtacagg tggtgattcg atcgatatga
     8401 ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa gcactaggag
     8461 ctaagattag tggtatcgat ttgattatac cagataaaga aatcgatcct acaactgata
     8521 aaaaagcata tgggatcatc gaagcaaatt tcaatccagc catgcatatg catgtttatc
     8581 cttttgcagg aaaaggcaga agattgacga tgaatgtatt gaaattgtta tatcctgaag
     8641 tattttaaaa gaaaaagctt ttgacagatt gtcctatttg atgtcaatct gatcaaaagc
     8701 tttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct
     8761 ttttttcttt tttggttttt ctttttcagc tggcgaagag ttttcttctg ggtatttttt
     8821 ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc
     8881 ctcaggattt gcagtgacgt aatcgttgac tacagtgaaa cggctattgt ttttcgttgc
     8941 tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc
     9001 atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc
     9061 agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc
     9121 ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc
     9181 ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc
     9241 ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat
     9301 aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga cgactagtag
     9361 cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt
     9421 cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac
     9481 gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac
     9541 atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt
     9601 taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga
     9661 cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc
     9721 tccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt
     9781 cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc
     9841 aagggaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat
     9901 tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt
     9961 ttgcagtaaa tctgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat
    10021 cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca
    10081 ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg
    10141 ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc
    10201 cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc
    10261 ttctgataag gggacgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt
    10321 gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat
    10381 tttgcatgtg cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc
    10441 agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta
    10501 ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga
    10561 gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc
    10621 gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta
    10681 ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa
    10741 gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc
    10801 aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg
    10861 ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac
    10921 ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca
    10981 gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc
    11041 agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta
    11101 gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca
    11161 acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc
    11221 gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat
    11281 cagtggttaa agatgtacag ttaacgattc ctgaaaagaa gttgactgct tttatcggtc
    11341 caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata
    11401 caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca
    11461 aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat
    11521 tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa
    11581 tcgttcagga atcactggaa caccttgggt tagaagaaat agcggatcaa tcgattgaca
    11641 ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg
    11701 attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca
    11761 tgcagatttt aaaaaattta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg
    11821 atatcaactt tgctgcaagt tatgcagacg aaattgttgc aatgaaagat ggacagttat
    11881 ttacgcaagg aacgacccaa gaaatgataa aaaaagatgt attggatgaa ttatacgaaa
    11941 tggacatacg gatttgtgag attgaaggcc gcagattctg tctttatttc taaaactaac
    12001 attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt
    12061 ttagcagcat gtggaaatag caacaacacc agtcaggcag acacaaaaaa aagcagcaca
    12121 caaactgaaa ctacattgac gataacagat agtaatggag accaaatcga ggtgccaaat
    12181 aatccagaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt
    12241 gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat
    12301 aaaaaagtag aatcagcagg tggaataaaa gaaccagact tagaaaaaat taatcagctt
    12361 cagccagatc taatcatcat ttctggccga caaagagatt ttcaaaaaga tctaagtcaa
    12421 attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa
    12481 aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag
    12541 gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca
    12601 ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt
    12661 ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac
    12721 ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta
    12781 gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg
    12841 gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg
    12901 tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca
    12961 tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt
    13021 acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc
    13081 gtattgatga ttttacggat ggtttttatt tatttgagaa atcttttttc aacgtgaatt
    13141 cattatctac atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc
    13201 attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta
    13261 gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc
    13321 atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt
    13381 ccagcgttct tattactcaa aacaaatcgc agttgtcaca atctcttcta aacgatgttc
    13441 catctgctgc ttctcggcaa taagcccact agactagaaa atatgagaag ctattttctg
    13501 tccagcgttc ttattactca aagcaaatcg cagttgtcac aatctcttct aaacaatgtt
    13561 caatcagctg accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat
    13621 gtgtctcagc aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa
    13681 gcttactcca ccgttttagt aattgataga aataactctc ttctgctttg attttatact
    13741 cgcttaatat caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca
    13801 atgctttggt tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga
    13861 tattttgtgt ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc
    13921 caaagaagtc ttttatttga taagtttact ctaccgaaga gttgttaata atccgtgaac
    13981 aaaaagtaat tctttttcca atagttgtta aaggtttttt attttttata gtattttttg
    14041 ttatttaaga aaggaataag gttaaacaaa taacaggttg ctttttgggt ctttttccgt
    14101 tcaagaaact gaacgaagaa aagggaaagt tgtctctttt gtgataagag aaatcataat
    14161 aaacgaatga cttactttcc aataaattga gaacagtcag aaccagacaa attagttgca
    14221 tctgatgaat aaagtgatta ttctatgaca tgaggtgata cagatgaaat tagaagtgac
    14281 aaaagaagca caagaagtat tgaagaataa attagaagca gacgatcaac ttttattaga
    14341 tatcgaaaac ggtgacggtc catttgcaga aagccaagtc agttgccaat tggatacggc
    14401 atttcgattg atcattgtca aaaaagatac acaaacagat ctaagtcttt attctgtagt
    14461 ggctgataca ccagttggac caatcaaaat caaagacagt gcaatacttt acatggatga
    14521 tccatccaca cttgctttag aaccaactta taatagttta caattaaaag gaccaagcgg
    14581 cttgagaaaa ggcaacttgc aagttgttcg aaaagactag agggataaaa tgggaaaatt
    14641 ggcgatcatc agcgacctac atgtggacat caataaattc ggcgaatcag agctgatgca
    14701 gctttggcaa gttttgcaaa agcacaagat cactcggctt catctagccg gagatacggc
    14761 taataaagta gaccgttgcc tagctgttgt cgaatttttc gtcaataaag gattatctac
    14821 gacatttcat ttcggcaatc acgagttggc agatgtgccc aatgaacaga tgatggacca
    14881 ttttccagac ccacattttt taaatgaacg gtatattgct ttaaatgagc agaccgttct
    14941 tttagggatg aatggctggt acgactatca gttttccgaa ttgaaagatt acgaggcaat
    15001 cgtacgaatg aagcgtttgt attggtatga tcggttcatc cagcgtggga aaacagatct
    15061 cgaggtcaat gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa
    15121 aaaacttgag gtgattttat ccactcattt cgttcctaaa cgggcattta tcatgtatca
    15181 atctgctcct tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag aatcattcgg
    15241 agaattactt gaccgttatt caaatatcaa acaagtagtt tttggccata cgcatcgtcg
    15301 ctttgaagat aaatccatcg gtcacacgat ttacagctgc agaccttttg gttattatta
    15361 tgaatggcag ctgacccgag attttgtctt gaaagaacag ttgctagaca gctatgatcc
    15421 tatgaaatta cgtgtcctct tgcgtgccca tcacccagct tttcaggcct atcaaacaca
    15481 acatttgcaa gaggaatttg aacaagcgat gacaatcgtt ccgtattgag acagcattag
    15541 taatgtgtaa aagaaaatac cttgtaaaac ataaggataa gtaagagcag tagatccatt
    15601 tatcgtggat atgctgcttt ttttgcggaa aataatcagc gaatgtttat ttgatcggat
    15661 gatttgtaac tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa cgataatcac
    15721 aaaaaaaaat ataatataca ttttttattt aattataatt aatattgatt gaagtctagg
    15781 cagtcaagtc acattttgat agataggagg gggacttacg aattatttta aaggctatga
    15841 cagctgtatt tctagattgt gtttgcatca agttgcaata aaaactagaa atcaaatcgg
    15901 gctgataatc atttataact aatcgataca taggaccaaa tataggtcta tgttctttta
    15961 tttcataagt atcgtaaaac agaattttgc tatggattaa tccaatcgac tgatatgttt
    16021 agtaaataaa gaaagaggaa tgtcaatgat gaaaaaagaa atcgtattaa cagcagcaat
    16081 gatgttattc agcatggtag caagtacgac ttttgtttct gcaactgaag tatacccaaa
    16141 ggaatataac acagaaggaa cgattacatt tgaagcagga gatgagggtg taactcctcc
    16201 tgttgatcca gagaatcctg atcccaataa acctgtcgat ccatcagatc ctccaagccc
    16261 tggaacagga ggggcattat cgattgatta tggatcaaaa ttcaaatttg ggactcaaaa
    16321 aatttctact gcggataaaa cgtattatgc agctgctgat gtgatgaacg atgggtctag
    16381 aaaaccgaca tatgtccaag tgacagatag gagaagtacc ttatctgggt ggaaattaag
    16441 tgtctctcaa ccagaacagt ttaaaactgc cagtggggat gaactagttg gagcacagct
    16501 gaaatttacg aaagggcagg ctgtttcttt ggttgatcca acatatacgc cgcaaacggt
    16561 gaattctgaa ttaacgttga caccgggagg aaacaatacg ttagcaatta atgctaaatc
    16621 aggaacaggt gtggggactt gggtctatcg atttggtgca aatgaaaatg aaaatcaaga
    16681 tgcggtacag ctttcggtac ccggtaaatc tgtcaagtta gcgcaacaat attccactaa
    16741 attagtttgg acattagaag atacacctaa caactagttt actgaaaaga actgttaaaa
    16801 ataaatacgt agtcatattg tcttgcgagg taatcgagca aaaattgtca aaggctaggc
    16861 taaatttgtt tggatacgaa tagttcatct aggaatttaa cgatcaggag gaaaagatat
    16921 attgaaaaag aggaggatcg tgctcatttt aagcagtctc ttagtgacta atatctttcc
    16981 gatcataggg tactctttgg aattgaatca gttggagcat ggaaatagaa gagacgaaga
    17041 aagcaaactc acttcgacat tcgacagttt aatagaatcc aaccagacac aagaaagcag
    17101 tgaggattta agtatttcga gtagtggaga gaccactgac aacgtagaag ctgagggttc
    17161 ggacaaacaa ggactagaag atgcaccaat ttctgcggtt gagctgcctg acgatcccaa
    17221 tattatttct atcgataaaa tttttcaaac acctatcggt actagcacgt ccatattgga
    17281 aaatgggaag ttactgcagt tgaacccagc agaaaggtct caaagaggag ctatttggtc
    17341 aaataagaag attagtttac tctcggattt tacatttaaa tcttatttat acttaggaaa
    17401 tcaacgagat agagcgggag atggcatgac ctttacattg actggcgatc ctagaatggc
    17461 tagtaatccc agtcaagtta ttggatcgcc aggtatggga attggctcat attctactcg
    17521 ttctggccaa ccttacattg cgaatgcgct ctcgattgag tttgatacgt ataaaaatag
    17581 tggttcttct gatcgaatgg atcgagagat aactgctgat aatagagggc atggacatgt
    17641 cgcttttgtc acgcctaaac caaataataa taattattct ggagagcata ctggcgtgac
    17701 ggtagctcct acatttcttt ctaatggcac atggcgcttg ttaataatta aatgggacgc
    17761 ggagcaaaga agtcttactt atgatttgga aggtgtcggt cgctcttctc ggattatacc
    17821 tgatctcatt aggcagtttg gttctacaga agtaaattgg gggtttactt cttcaacagg
    17881 tacttttttt caagaaaatg ctgtcgctgt cacgcgactt ccatcgaatg tcacatcaac
    17941 agctgatgta aaagtcaacg atagtgatta tggggccatc gctgaagctg gaggaaacga
    18001 taagataact atcagaaata cattgaaggt cgagggggat tttcttgaat ataagaaaat
    18061 gcagactagt atcgacctcc cacaagaatt aactattccc gagcatatct tgatcaatgg
    18121 aaatcaaatt gatgccaaag atatcttgat aaccggaaat cgagtcacgc taaatctgac
    18181 agattatttt attgctggag atacgttaat aatcgaattg gagacaactt tgcaggaagt
    18241 aattcctgaa aaagtactca ctatgcattt tgaatacctt gaaaacggga atttgattga
    18301 gcaatcgaat gaaataaacc taaagatcgc aaagctgaag gagatagata tccatgttta
    18361 ttataaagat aaagacagtc agattgatat tgctgaaatg aaaacaatca ctgggaaaat
    18421 aggagagaag tataaagaaa ctcctattga tattgaaggg tatgtttttg actcggattc
    18481 agcgaataca gaaggcatct tctccgaaaa tacagaggat atctattttt attacagaaa
    18541 aggcaaactt tatctctcaa ctgctcctca atatgtcaat tttggaaaac ataaaatatc
    18601 cagtagacca ttaagtgaat tcggacagtt ttcaggtggg ttgaaaatta tagatgaacg
    18661 ggcttttgga agttggaaat tacaattgaa acaagagaaa gttcttacaa atggagattt
    18721 tgagcttcca gaagctcttt cctttgttac ctctgaaaat acaacggtta tagggacatc
    18781 cgctgttacg attttcgaaa gtgatcaaaa gggtgaaagc gatttgtcct ctcttttaga
    18841 ttctacagct catcgtggaa tcagaataaa tattcctgta gaaaaccaaa gaatcggaac
    18901 ctttgagggg aaactatctt ggatacttgc agatgtccca ggaaattaac aggaagagaa
    18961 gaatataaca aaattattga gtttcaagaa gggcacaggt accataggct cttgtgtccc
    19021 acagagacaa gattttgtta tagtcttctc ttacatatga ttaaaggagc gattttcttg
    19081 agaccaaaac aacaattatt tataacactc ctagttattt taggggcact ttttaacaat
    19141 atatcagtgt cagctagtgc atttaatttt tcagtgacgc cgataacttc agagaatcaa
    19201 atcgacaaga gaaaaactta cttcgatctt caacttgatc cggatcagga agtagaagta
    19261 aaagcggaat tgagaaatga tacagaaaaa gaagtgaaaa tcgatatttc tgtgaatagc
    19321 gcaaccacca attcaaatgt gatggttgaa tacggaaaaa acgagattga aaaagacatg
    19381 agcctgatct ttgatctggt agattatgtg agctatccac agatagtgac gctaaaacct
    19441 aaaagtgtcc aaaccgttgc ttttcatgtc aggatgccga atgagcgatt tgatggtgta
    19501 ttggctggtg ggatcacatt ccaagaaatc cagacggaaa aggaccaaac agaaactaag
    19561 gatcaaagct tatcgattga gaatgaatac acctatatta ttgctttgtt gatgcaacag
    19621 acactgaacg aagttgctcc gaaccttact cttcatgaag ttaaaccaga tcaaatcaat
    19681 gcaagaaatg tcatcttagc aaacgttcaa aatgatcaaa aaacctatat caatcaagta
    19741 gttattgaaa caaaaattac aaaaaaagga cattctgaag ttttatatca agaggaaaaa
    19801 gaaggattgc agattgcgcc gaatacaaat ttttcttttc caactgcttt aaatggtcaa
    19861 ccgctaaccc caggtgaata tcaccttaca atgactgttt taggaaatga aaatgaaaat
    19921 ggaaaatttt cgagaaaaaa aggaaatact acgatcaact ataccaatca atgggtattt
    19981 gaaaaagatt ttacaataga cggaaaagta gccaaggaat tgaacacgac ggatgttacg
    20041 attaaaaaag acaataattg gatttatatc ttagtagctt cacttttctt attgtttatt
    20101 ttattatgca tacttttgtt tgttttgaga aaaaaagaag agaaagaaaa gtaaggttaa
    20161 gataacaaat agtaaatttt tattattgta ggatggttgg gctcttttta taacaatata
    20221 aaatatacca ttttattaat aaaaatgcat actagtaaat gatataattc taataacagg
    20281 tttgatcgcg agaaaggcca aataaaacgc tctccaaaat gttaaaaaaa agacaggacg
    20341 atgtatatta tacaaacaag aatcgctgac aacagtgact cggatccagg aaatcagttt
    20401 tatccccagc tagttggtga atgcatggaa agtaatatac gtagaaagag ctgatgacta
    20461 atgaattatg agtttttttt aagcaaagaa gatcacagaa gttattcatt acttaaatat
    20521 ttagaagcaa gctcaaattt atcagagtca atatcgaatg ttcaagaaga gttgtcgcta
    20581 tcgacatttc tcttgaaaaa aacgatcgat aagctgaaac atgatttaca aaagttcgat
    20641 ctagagaaaa atttgcgagt aaccgtcagt gaattggata tatgtctgga aattaatgga
    20701 aaatgttcta gcaaagctct gttatctcgt tatataacgg attctttatc tatgaaaatg
    20761 gtattggctt tctttcagga aaaatatatc tcagtagaaa attttgctaa acagcatcac
    20821 gtgagttatt ctgtggctta taaagtatta caaagactaa aaagaaatct aaaaaaatat
    20881 caaatttttt ttgaaa
//
LOCUS       DAYYKN010000044        20626 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000044 DAYYKN010000000
VERSION     DAYYKN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.044"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACHBB7_001320"
     CDS             complement(<1..272)
                     /locus_tag="ACHBB7_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209280.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="ACHBB7_001321"
     CDS             complement(309..446)
                     /locus_tag="ACHBB7_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM9209281.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..879)
                     /locus_tag="ACHBB7_001322"
     CDS             complement(448..879)
                     /locus_tag="ACHBB7_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209282.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(893..1387)
                     /locus_tag="ACHBB7_001323"
     CDS             complement(893..1387)
                     /locus_tag="ACHBB7_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209283.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(1387..1836)
                     /locus_tag="ACHBB7_001324"
     CDS             complement(1387..1836)
                     /locus_tag="ACHBB7_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209284.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTMVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(1840..2457)
                     /locus_tag="ACHBB7_001325"
     CDS             complement(1840..2457)
                     /locus_tag="ACHBB7_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209285.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(2457..3365)
                     /locus_tag="ACHBB7_001326"
     CDS             complement(2457..3365)
                     /locus_tag="ACHBB7_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM9209286.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(3378..6137)
                     /locus_tag="ACHBB7_001327"
     CDS             complement(3378..6137)
                     /locus_tag="ACHBB7_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM9209287.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMDVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(6134..6838)
                     /locus_tag="ACHBB7_001328"
     CDS             complement(6134..6838)
                     /locus_tag="ACHBB7_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9209288.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(6850..9159)
                     /locus_tag="ACHBB7_001329"
     CDS             complement(6850..9159)
                     /locus_tag="ACHBB7_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM9209289.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(9354..9818)
                     /locus_tag="ACHBB7_001330"
     CDS             complement(9354..9818)
                     /locus_tag="ACHBB7_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209290.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(9818..10444)
                     /locus_tag="ACHBB7_001331"
     CDS             complement(9818..10444)
                     /locus_tag="ACHBB7_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9209291.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(10451..10816)
                     /locus_tag="ACHBB7_001332"
     CDS             complement(10451..10816)
                     /locus_tag="ACHBB7_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM9209292.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(10806..11144)
                     /locus_tag="ACHBB7_001333"
     CDS             complement(10806..11144)
                     /locus_tag="ACHBB7_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209293.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(11134..11460)
                     /locus_tag="ACHBB7_001334"
     CDS             complement(11134..11460)
                     /locus_tag="ACHBB7_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9209294.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(11441..11719)
                     /locus_tag="ACHBB7_001335"
     CDS             complement(11441..11719)
                     /locus_tag="ACHBB7_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9209295.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(11721..13085)
                     /locus_tag="ACHBB7_001336"
     CDS             complement(11721..13085)
                     /locus_tag="ACHBB7_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9209296.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(13098..13667)
                     /locus_tag="ACHBB7_001337"
     CDS             complement(13098..13667)
                     /locus_tag="ACHBB7_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM9209297.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(13639..14826)
                     /locus_tag="ACHBB7_001338"
     CDS             complement(13639..14826)
                     /locus_tag="ACHBB7_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9209298.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(14890..16617)
                     /locus_tag="ACHBB7_001339"
     CDS             complement(14890..16617)
                     /locus_tag="ACHBB7_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9209299.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(16614..17066)
                     /locus_tag="ACHBB7_001340"
     CDS             complement(16614..17066)
                     /locus_tag="ACHBB7_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM9209300.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(17178..17558)
                     /locus_tag="ACHBB7_001341"
     CDS             complement(17178..17558)
                     /locus_tag="ACHBB7_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM9209301.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(17555..17941)
                     /locus_tag="ACHBB7_001342"
     CDS             complement(17555..17941)
                     /locus_tag="ACHBB7_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM9209302.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(17943..18221)
                     /locus_tag="ACHBB7_001343"
     CDS             complement(17943..18221)
                     /locus_tag="ACHBB7_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209303.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            18269..18997
                     /locus_tag="ACHBB7_001344"
     CDS             18269..18997
                     /locus_tag="ACHBB7_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM9209304.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(19143..19619)
                     /locus_tag="ACHBB7_001345"
     CDS             complement(19143..19619)
                     /locus_tag="ACHBB7_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM9209305.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(19579..19935)
                     /locus_tag="ACHBB7_001346"
     CDS             complement(19579..19935)
                     /locus_tag="ACHBB7_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209306.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(19913..20098)
                     /locus_tag="ACHBB7_001347"
     CDS             complement(19913..20098)
                     /locus_tag="ACHBB7_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209307.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(20099..20371)
                     /locus_tag="ACHBB7_001348"
     CDS             complement(20099..20371)
                     /locus_tag="ACHBB7_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM9209308.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(20368..20568)
                     /locus_tag="ACHBB7_001349"
     CDS             complement(20368..20568)
                     /locus_tag="ACHBB7_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209309.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
BASE COUNT         5779 a         4392 c         3158 g         7297 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcataatg gaacgaaaac cattacttgt tcgttagtga aacagcccca gtcatacatt
      421 agtttgatat cttcatatga atacatgtta cttcccacct tgtaattgtt ttttgatttc
      481 ttcgatttcc ttggtatttt gtacagaagc aagcatcgtt tttgaattca actgcgctaa
      541 actttctgac ttttctttca ataagacatt ttcttgcttg attgctacat tgtttagcat
      601 tgctttagaa tttagctgtt tcaattcatc attttctgcc ttcagcgttt cgtataatgc
      661 cttaatatta gccaattcat ctacgctatc aacagcattc ccttttttct cttcttcagt
      721 agctaactca acccactgtt ttttatcaaa atcaaatctt ggtctccagt taggaactgg
      781 tggtttaatc tctgtacagt tttcagggat attttcttga ttgttcaaaa tgatttgctc
      841 gaatccgtaa ggcttgattg atttgtaaac tactttcatt agatttcctc ccttaaattg
      901 aataagtgat aacaaatgaa taagctgatc cataacttga atttcttctc catttaatgg
      961 ctccgtctgc gccaatagat agttgagcac tgttcaaagt agaacggtct attgacccaa
     1021 ccagttgttc aaaactaatt ggtgtccgat agccttctgg aacagttaaa ataaccgaat
     1081 cattcccgcc actacttttt ccgtttaaag ccacaaaata tatagaaact gtttttcctt
     1141 cacgataaag ctttgctgtc cctgtattac cgttagtgac aaccagattt accacttcat
     1201 atctgttatc atcagtcgtc aaaacacgtt tattattgat tgttacagaa cctgaaaaat
     1261 tcttatgtcc tgcaatggct tggtctccat ctgtctgcac caatgcttct ttaccatcaa
     1321 tagcttctac atgcgttttt aagtatttag caactccgtc ttcttttaat tgaacaatat
     1381 cagccattaa accgtcccca ctttctcaaa tgtaatattc gctaatccat caagtttcac
     1441 tttatcggtt gctgacatta aacctgctgt cgtagttgta gcattacctg gatttttctg
     1501 tgctccagct gcaattccat ccaacttagt tttatctgtg gacgacatca acccatttgc
     1561 tgtagttgta gctacagctg tagttgtggc atttattcca ggatcacctt tatctccctt
     1621 tggtaaaaca aaattaaatc tagctgcaga tgatgttcct acattcgtaa cagaagcggt
     1681 tgaaccacta gaaacggttc ctatggtaat tgttgctgct tggccaggat cgcctttatc
     1741 tcccttcacc attgttggtt tgccttctat agcattccaa tgagtttgtg gataaacctg
     1801 tacattgctt tgttttattt ttacgatatc tgtcattctc tatacctccc caattttttc
     1861 aaaagtaaaa tttggaattc tttcgtttgt gtaattttct gcttgattta cagcttcttg
     1921 gaatttttga tctacatatg actgattaac accaccagtc ccactaccac ctgtagaact
     1981 aattgttcca tcttctgcaa ttgatatatt tgcacctgct tttaatattt ttagagattc
     2041 taattttcct tttaattctt cagagaaatt gaagtctgtt tgcttagttg cagataaaac
     2101 accttcttca gtaacttcta agagttcccc aacttttata cctcctaatt gttcaggagt
     2161 agcaatcggc aaaatatatg ttccaccttc tccatttacg atcttttgaa acatttcagc
     2221 agtaagaata ccatcgcttg tctcgcttgc ataaggtagt tcagtaattg cattctctaa
     2281 tcctaaatca gctttcgtta aaatgactgc accatattta ccattaaccg aaagaacttt
     2341 tgattgtcct gatatcattt tttctaagcc taaaactgca gagacatgtg taattggcat
     2401 aaattgacgt ttaacaccag attcatcagt ttccatcatt ctctttactt taaccatcaa
     2461 atcaccccaa ctttctcaag agtgaaaaca ttctgttttg gatcatcaac agttgcaata
     2521 atcaaagctc cttcttcaat tggaaattct actgtttcaa ttttttcgac ttcgtgatta
     2581 tctgaaaaga gatcgtcttg aagaatatca gtcacttcaa tttcaccgta ttcaatcgtg
     2641 aaaagtgctg ctttcatttt ttgatacaaa taatttagat cggataggaa acgttcagaa
     2701 atcgattcat ggcgtacacc ttgaagatct acacgtgcat ccattaattc ggctaacatt
     2761 actccacctg gatcgataga cttcaaaata tccttgattg attcaaacca tgacaagtaa
     2821 tctgattctt gtccttctct ccaatcttgg aaactattct cctgttcctc tctccagcga
     2881 tcaaattctt ctttcctttc attcatccag tccgtaaaat cgcctttatt ttcattaata
     2941 aaatctgtca tatcagcaat taaatcttca attgattgcc aataagaacc catttctcct
     3001 tctgtttttg aaacagcatt gatgacaaaa taagagaagt cttgagttgt tccaatcaag
     3061 tcttctcctt taaatataat gaaattggct gtttgtcgat gcaaacactg catggaatat
     3121 ttatcaaaaa tatatttgat tttccctttt ttagcatcca caatttttgt ttctaattgg
     3181 actggatatt ttccgccaac aactgattca aaatatacct tacattctga taaatcatat
     3241 ggaagaccat tttcgacaat tgtagcttcc ataacttcag tgtttttatt gccttgtcga
     3301 acttgaatca tccccacgta attatagggt tctgttgtac ttaatatgac attccacttt
     3361 gccattaaat cacctcccta ttttggtggt atgacaatgg aagaaattgc acttgcacta
     3421 taatactgtc gatctaactt tccacaaatc atacctaact ctgtgttttg ttcataagtt
     3481 tgcatacgac cattagctaa gcctcggata acgccagtat gtccataagt tccatctgca
     3541 aaccatgaac ccacttgtcc acctcttgcc caattaataa ttgcaccaac tactaattga
     3601 tcatatctag ggttttgaat cactttccaa ccaacagcag accaatcata ggcaatacca
     3661 atatcagatg ctgcagaagt atttcctatc acatgagtta aaccatattt tgttccagca
     3721 cccatgccac aaccacccag atatcctgaa tattctgcag acaagccata acactgacca
     3781 ttgccaattc gttgtccaat caaagactct aaatgtttta gtcctgcttc tccagtagct
     3841 cctcctggtt ttaaatcttt aaatttgtta taccagttaa ctgcataatc ttgacgttca
     3901 ggatgtgtcg ctgccggacg ttcatagttt cgttcaaaag catatgctgc ttgtcttgga
     3961 tcagtacagg ccttaaatcc atcaaccgtc gtaggttgta ctacgtccat ccattgtcca
     4021 tttgtaaatg tccaaataag caatcgaact tgtgcatcta agctcgttat tggttcttta
     4081 atacctgcag cattaaataa gttttgaaca taaacttttc catcccatgt tgctggacca
     4141 acaagaggat atgaagaacc atcccactga actaatccgt atgctggtcc acctatttga
     4201 acagtatcag gatcaaaagt tcctcctgtt tcttgttgaa tgttccccaa tattccacat
     4261 gcagattgtt tcgtaaatcc gttattacac aaaatatcgt atattctcca agctctcttt
     4321 tctgcatctg tttttaattc atcaggatac ccaccaggtg tttctcctcc gccagatgga
     4381 ccgccatttt gtcctggtat cacttcttta cctctaacag taagttttcc ctgaatatct
     4441 aaatcaccga aataatatgc ttttccgttt cctaataaca caaatccttt ttctgcagtt
     4501 ggagaaatta aaatgtattt tccatcgcca tttgtacgta ttaccaaaga attttcctca
     4561 agaggtgtag gtgttacagc atttggaaat ggatttcctg ctgaatctgt tgttccgatc
     4621 gttccaatgg attcttttct attccagaat tccatacctt ttttggttag ctccatgatt
     4681 tttttgtttt cattccaaat ttgaagcgtg ccttttacaa gttttaacac atcaccataa
     4741 gcattaaagg aagtttcgaa tacttctgca tttattgttc ctactttgat gaagtcagca
     4801 acaatttctc cttttgatgt gatagcaata ccgaacgggc catttactcc gttgtctgaa
     4861 taacctaagc catttaaatt ccaacgccac actcgctttg cattcgccac atttggagta
     4921 tccatgataa gaatctctga tggagctttt tctggacgaa aaacgacatg cccaccagaa
     4981 ttaccagtga tccatgccgt cgcattcaac acattttgaa ctaacgtttc tgttcgattg
     5041 tcgattttct ttttcaattc ttgtgcttga gtatttactg ccgaagtgta cagtgataaa
     5101 tcattaccta aaacaatatc cttatatttc ccaagcgttg ggaaataagt gtattcgacc
     5161 atacgctctt ttatctcgat atctagctct ttcgccctaa catgagcgac atcaccaaag
     5221 tgtaaagacg ctagctcttg atacatgtca ccgtattcga gtgtgtgttc caacgccacc
     5281 atactgactg tatgagtagc ttttggctca tggatacgat cattgtcaaa taaggttttg
     5341 ccccatttga tcaattcatc aactgtttta caatctccgt tttcacgttt ggcaatacgt
     5401 cgattctcgt tggttactcc atcaatttct aaataaccat attcgatagg ttctttgtct
     5461 tcatcataat cgttatcagg aacaccgcca accaaataca aactattgac aattgattct
     5521 tcatcaattt cttcttcgat tgcttctaaa ttgatgccaa aatcaatccg aaaaccatta
     5581 tctgatccaa tctgttttac cagtttcaaa tcatagttat ccatatcaag ttccgcgcta
     5641 gtcacgccag tcaaattttg attaccgtta tttgaaccaa tgattgcttc aactggcgcg
     5701 acttgtcttg ctgtaaattg gtgcgttgtc cctacattcg aaagatagtt aaatttttga
     5761 tcaaatgcca aactgttttt aagattggtc atgatctgag acccattgcc gttatccgta
     5821 aacgacttga caataaagtt cttattggcc ataaaaccaa tatgccgagc tgttacagaa
     5881 acggaggtca aattcttttt gacattataa atttcaaaat attgccatga tccatctggt
     5941 accttcgctt ttataaaatt tccttttttt aaataagagc gatactgtcc gcttcttgaa
     6001 tagttaccat aaaaacgata ttggccattc agcacacgat tgatttctgg caaatcttcc
     6061 caatcaacta acgacgctcc gttcacgctc aaatcatctg gcattttttt ataagcataa
     6121 ataaattctt gcgtcacaga tacacactcc tattccaaaa ccgcacttct ttaaattttc
     6181 cggatatttt tacctgattc cattcaggct gcaatactgg ccaatcacca cgcgtaaata
     6241 aattcaaacc ttcttgtatt gcttttccaa gctgagtatc aaccacgatt gttgcagcta
     6301 acgtattaag tattgttagg cttttatcac cgacagcgat cgttatatct cctccgttcg
     6361 attctatttc cagataggga tgtgcaattt cgtcaccatg atcaaaaata tccataacac
     6421 tagaatgaaa aattttaggt gcttcaccga tttttctttt gagtggctga caacgaaaag
     6481 taacatcgaa tgtataaaag aagccccact cgttttcgaa agggacttct ttatccatgc
     6541 tgcagatagc ctctagatac ttgtctggat cattatgtgt gattaattgg cttttaccag
     6601 tgagccatcg tttgacttct ctcagtttcg aatgtggaat agtgatccct tctatttcca
     6661 aatcaaaagg ttcatagtca tcaaacgtct ccgtcaattc tccgcttcgg ccctgaatcg
     6721 tgtaagtttc gtatcgcttg ttcggcataa tatctggaag ctctgcctca atgatgcaat
     6781 tcatatcaat tactgcattc cgatttttcc aaataaaatt tggttcatct ggattcataa
     6841 attgtcgact caaataggaa cacctcccaa atcacgaata gcctgtttgt ttgctttagc
     6901 aaatttacga ttcatacgat ctaactcaga tggattgttt gcatcgactt tgccaatgtg
     6961 tatatgctgt tcaatgctac ctccagaaac tcttccactg atccctttgc gtttttcttc
     7021 gtctgataat ggagtaactg tggttttacc attcttggca gtcagtaact caggtccagc
     7081 ttcaccaaca atcgcttgtc cattgattag atgaccgcct tgtgctaaat aaggcagttt
     7141 tgaaatagaa aatgattttc cacctacacc aggaacccat tttggtatct ttatattatt
     7201 caatccacca ataaatccat tgatcaatcc aatcatggca ttaataggag ctttccctac
     7261 agcaacaata ccatcgaaaa tacctccaaa aatatcaact agaccttgcc atgcttttga
     7321 ccaattccct gtaaatactc cagtgataaa atctagaaaa ccactgaaaa ttcgttttcc
     7381 agcattataa aaattattga aatttgctat aacaccatcg aaaaatccac caaaataacc
     7441 acttaaaaaa ttaaatattt caacagctac atctgaaaca cctttgaaga aagaattcac
     7501 tccatcatga aaccatttaa cgttattata tgcccaaatt agtccagtaa ctaatgcgcc
     7561 aatagctata actgctaatg caaaccagcc tcccgacatt ccaaataaac cagcaagtcc
     7621 ttgccaaact cctataaaac ttttaacttc cccaacgatt tttgtaacag atcccataag
     7681 tgaaccaaga acgattagta ctggtccaac tgctgccgct ataccagcaa ttgttatgat
     7741 ccaattcttt gtatcttgat ctagagaccc ccaccattta gagaattcct gtaataagga
     7801 agttgctttt tcaaaaaaag gcaaaaggct tacttggact gcttcaccta cgtcagccat
     7861 tgctaatttt gcattgttca tagcttgatc ggcttgatca attgggtcga gagtagcatc
     7921 gaatgtgtct cctacagctc ccccactttc tccagcagtt tttgctaaat cttccaggtt
     7981 caaagtacct ctacgaattg catcggccat tctaggaccg cctttagtcc cgaatacttc
     8041 tgctgctgca ttgatggctt ctgtttcaga acctgcattt tttaccttgt cttgtagttc
     8101 ttctagtcct tgacttaaag attttccgtc tttcgcataa gccactgttg ctttagataa
     8161 actacttaaa gctgcgcttg aatcaacacc agattgctca aattgtccaa gtaaagtgac
     8221 cccttcacca aagctcaacc ctaattgttt aatttgtggt gcgccatcaa ttgctttttg
     8281 catcaagtcg tctacagatt gaccagtatt ctgcgatgtt ttcgttgtta catcaaggac
     8341 agaattcaaa tcatcatatt ctagtccata agcatcaata gcttgtcgag cagatattgc
     8401 tgattgcgaa acatctgtat cattgatttc agcgtactgt aggagataat tggttgaatc
     8461 ttccagtttt ttatccatga atccaaattg tgtatttacc tcaccaatag cttccccaac
     8521 cgtttgtaaa ggtaaatgag tatttgaacc aacgtttttg aaagactgtg aaagtctatc
     8581 agcttgatca cctgttgctc cggtttttgt aatgatggta tcaagtgcct cgtcaacttc
     8641 accaaatgct gcaagtcctg ctgctcctgc cgctacaata ggcgcggtta ctccaacaga
     8701 cattttttca ccaacgcctt ttactttctc acctgtttct tcgattttct ggagtttttt
     8761 tgctgtatca acagaaacgt caccttgttc tttaagtgca tcgttcgtct gttctaatgc
     8821 tgtacgaagt ttattttctc cggtttcaga attgagtaat tgtttataaa gtttttctga
     8881 ttcagcagaa tattcaccgg tttctttgac agatttttca tattcctctc tcagcatttg
     8941 agatcgttgt tcagccaatc ctaattgttt ttctaatttt tgtttagccg ctcgcaattt
     9001 ttctgtttga gttgcatcct tgtccatcgc agacacttgg tttttgtact cggcagcggc
     9061 taagttcatt tctttgttga tttctttgat tgttttcgaa taactgactt cgccattcgt
     9121 tttaaaatta agaacgacat cagattcttt accagccatt tatcttagcg ctcctttcta
     9181 ccaccaaggc gatttatcca tagttacaga ttgaggtggt tcaaaatcgg tgttttgttg
     9241 taaccactgt aaataagatt tgagccacaa gttaggtgta gactttaaaa agaaactctc
     9301 actccaattc aatagagtga gagcaacgta aatataaaaa ctccaaggag ttcctatctc
     9361 ttccgattct ttttgttttt ctttcttttt gcttgcgctt tttgaaagtc ttgtggcttc
     9421 ttggattttt ttaggtcttc cacctgaaat gtctggctag caaaaatttc catacaggta
     9481 ctataagcag acaacacctc tccactcatt cccaaaaatt tgaaaatagt ttctggatct
     9541 tcctctaaac caccagtacg cagcatggca taaatcaagg cacgcatgat ttttagatca
     9601 ctaggcgata agttagcaga agaaatttgt ccttcttttt tagatagcat ggcgttcata
     9661 tcagattcaa attgcgaata atctccacca tacacatctg ctataaattc catcgtttgc
     9721 atagtaaatg aaatagggaa ttctgcgcct tgaataatta caacagcaga attttttaag
     9781 tcttcaacgt gaattccata atcagataac cgagccatta acccgcacct ccaactttag
     9841 ccaatgtttt ccactgttct tcatcgtata caggttgagc aatgaattta ttaaataatt
     9901 ctagagaagc tccgtctcta tttgaatcaa aacttgcata catcacatta ttgtatttca
     9961 gtccgatgga aaccagattt gcagttacat cgtcaatttt tgtttcttcc tctgcagtgg
    10021 catactcttc gtcaatcaca ttggatagtt gtgtattagg ataccaaact gcttttttac
    10081 cgccattttc aatatttcca ataaatccaa aggcgaagta cggaaattct cgtgcggtat
    10141 ttttgctaaa cgttactccg cttttagcaa ctaatccttt gatttcatcc atcacttcta
    10201 tcggaatacc cacgtgatcc aacgcaagtt catgttttgt ttctcgcgac acacgacgaa
    10261 acattttgct ggatgcccat ttttctaatg ctgttccatt ccctttgaca ccgatttttg
    10321 tggcaattgg caatcgtaaa acttcactat aagtcggtgc agttccaaca gaatctggcg
    10381 ttgccatcat cgcaattaaa atgtcgtcca atccttcaaa ataaaaagta tcttgttttc
    10441 ccaattaaaa tcacccttcc cataaatcaa gtatttttct agtcataatt tcttcaattt
    10501 tttctttgtt ttgttcatac gtaccacttg caaaatgttg ggcacgttga tttgttgtgc
    10561 cattttcggc aaagcgccag taaaaggccg tctcttcaaa ttgaactttc acttgatcgt
    10621 tttcaactac tacttttatt tgatcgcgca tatgtttctt ttttaagagc gacttaggaa
    10681 tattaggaag caactgcttt agataaaagt tagctgcttc ttctaaagat tccaacgata
    10741 attttttcgg atcaactttt gaaaggtttc ctaaataatc cgatatttca gaaaaaccat
    10801 tcttattact tggcattttc cacacacctc acatatgtgt aatagttggt tacggtatcg
    10861 tcgttctcat caccttgtat acctgtaaaa tctgaatatg gaatgccagc attttgcagc
    10921 gcttgttcaa taacaactaa atcctgttct gttccaagtg taaaaaaaga gacttggtaa
    10981 taaggtaatt ttttatagac tttaccggat gccatttttt tgctgttact cacatttgag
    11041 tacacaatgt atggatacat cgtccctaat ctggctttgt ctctgaacac tgctaacttt
    11101 gttgatttca gcgctgtttt caattcatca aagctaatcg acataagcta aactcaactc
    11161 catttctctt gcatcgggat tcgtataaat gcgagtaatg ttatacgtta cagaatcaat
    11221 tttgagcgca cttaatttct ctgtgatgga tttatcccat ctgactttaa ttcgtctgac
    11281 aacgtctgtc ttggcttgct gtgataaata tttttcttga gaagtcacac cgagttcttc
    11341 atagaatatt agacgcttaa attcgtaaat tgtagttgga cgatcgtttc cgtctgttcc
    11401 tgttttgatg tctagcaatt cggctttcca tctgagatta ttagtctgtc tcttcggcat
    11461 tttgaatcgc tccttgcacg ataaatggcg tcatggcatt catagctttg tcgagttcat
    11521 cctctgaaac tctatattca taggcaatgc cggcaaccat caaaataaga tattcttgtt
    11581 ggccaccagt tgctgttttg acataatctt ttgccatatt taaataaaaa gagagcaaag
    11641 aatcatccat gccctcttca aaatgaatat gtgatttgaa tttttcctct aaagacaatt
    11701 ctttagtttg ctcttccatc ttaaccacca actggttttg taatttcgta gcgatatact
    11761 gccggttcaa atggagaata aaccaattga ccatctagca agttgtaaat ttggaatcca
    11821 atttgatttt taccagagaa tttttcaaca agtttttgaa tttccaaggc accaataact
    11881 tcttgaattt taaatgcaga aaaatcgcca aaatataaaa ctggcgtgtc tggttcaccc
    11941 tttttatctg ctgcatctgt ccaatccaca ggatagccaa ctaattggta accaattcca
    12001 ccttctgctt gtgtgaatgg ccgcaacaaa ggaaatccat catctgtttt cattttttca
    12061 atggcagtca aagcagctcg attaataata aagcgtccct ttttcatcac ttctgtcact
    12121 ggtgtatttt taaattcgat taatgcatca tataattttt gcccagcacc tgaagcagtt
    12181 agatctaaag gttttttaaa tgctacagcc tttttggcta atgcaccagg attttcattt
    12241 ccagcgtcat caccattgaa catataattg atttctttac gcacataagc ttttttcaat
    12301 tcttccacaa caatatcttc aactggaaca ccagacattt ttaataattt tttagttact
    12361 gttgccaaag catcgaattc ggcaggatta agcaaaattt catcaaactg aatagctgtt
    12421 tcagcaatat cagttgaacg ctctttcttg tttacattcg catctgcttt cttcacaaga
    12481 attggatatt tgacatctcc tgatgttcgc accactgttc cgtatttacg aagtaaattt
    12541 tcttcttgag cataagtaat aacttcagat gcaattactt ctgggacagt aactgaaccg
    12601 ttgccagctt caatccctaa agctcgagct tctgcttcag aaatatttcc agctacaaaa
    12661 ttagcaaatg cttttcgtag ttgttgatct ttttgtttct tggtcatttt tgcacgtgcc
    12721 tccaatccgt tcttaattga tccaagtaat ccatctcgtt gctgttgagt aatcattcca
    12781 gaacgatttt ctgaactatc ttcactgtct gaatcttctt cttgatcgtt gtcttcacca
    12841 gaacggcttt cgtctgaatc cgtactattc gattgatcat ctgtattgtc gtctgtttca
    12901 tcagttccag aatctgcgcc taattcgtct ttaattccgt tcaattcatc aataacactg
    12961 tcaatttctt ctttcacggc ttctaaatct gcttcgcgta attctcctga ttcaactttt
    13021 tcacgtaatt cagtcaatcg ttgctcacga cgtgccttca tttttttcaa taattcttta
    13081 tccatgtatt tttcctccta cgcttctagc gcttgattaa tttttttgat taattttttt
    13141 ctttcttcga cgtttttttc taattcatca cgacttctta atgcagcttc tgtatcttcg
    13201 tatgctggca aaggcacaat agaaacctcg tataattcca cttcatggat tgttcgaagc
    13261 attggttcag aattgtagtc ccacgtttct tcagtcggca caaaaccaaa actacattgg
    13321 ttgatgtctc ctcgttccat agatttaact aagtcccttg ctaccgttgt gtccggtaaa
    13381 tcaacttcga atttcaaacc acgttcatct tcttcaaggc gaagggttcc gcttttagta
    13441 cgtcctagca cattggacca atcgtgattg aacaaacaac gaacatccga attactaatt
    13501 gcctttgaga atgcaccagg aacaatcact tcgcttaagt catcccataa aagtgtttga
    13561 ctgttgaata cagcagcgta gccgctaatg gtcctcgtat gagtttcttc atcggaacgt
    13621 gttgaaagat tggtgatgtc aatcgtgcga atttccttct tcttcatttc catcacctcc
    13681 tttcaagtct tgatcattcg ttggtaagga atcatctgtt gcatttttct ggccaatcct
    13741 agataagtca tttgaaatat agatagcttg tgtttctgga gtattctgtt taggaaaacc
    13801 aagcatttct gccacgttat ctggacttgt aatcccggta cgaacgatgt tgtagccaat
    13861 atttgttttt gttgagtaag gaacaaaatc caaaatatta attttccatt ccactctata
    13921 gccagaatta ggcataaaaa aaagagcggt gtaatgttcg ctcttgtttt tcaatattgg
    13981 tttgattgct ttgttgtgca gatacatcat cgctttttca atatctgtct tcatcagcga
    14041 ttgatacgta tcaacattga ttcctagaaa ttttcctaag tcttttttgt aaacacctaa
    14101 ataattcaaa attgccgcgt catcaacagg actttttaat gtctcgatgg aatatccttt
    14161 tcccaaagga atcatcttaa cagaatgatc actctcattt tgcgttcctt ccagttgatc
    14221 caatatagct ttgacaattt ttgtttgggc gctattattt ggattgatgt gggcgtctag
    14281 tttaagcatg aacgcaagta aaccgccttt agtatatttg tccgtcaaaa ctttttcagc
    14341 gctcagaacg ccttctagag tgtttcttgc aagatcaatt attccagcac cttttaatga
    14401 atcagttccg atgttcttaa tgtgtcgaat catttgacca ggtatttttt gaccattcat
    14461 ttcaaattct tcttgaagtc gttcattgat tttagttgtt acaccgtacg ccaaatgaag
    14521 ctgatcccta tccgttaatg ggaatgtctc accattgatc aataaagtat ttgtttccaa
    14581 tttggtaaat tcgaatcctg tcaaataatc attgggactc ttcaaaatat ttagtaagtg
    14641 atgatctttg acttcgttgc cgtcgggacc aataacaatt ggtgtggcca atgccacctg
    14701 attagatatg tcttgaacta attcataaac atcagaagat tccatgatag atgaatccgt
    14761 cacatatctt tgaccataac gtgtgtagtg tccaaaaata tcctcgatat atccacgttt
    14821 ttccataaaa gaatagactg cattcgataa ccgatcacgt aatttcaatt tttttcaccg
    14881 cctttctatt tatctataga tggaacttag gtaatcatct aactcatctg aatcaatatc
    14941 tgtcatttga ttcatcgttt ccttatgacc acacaaaaac gccacgaacc catcaatctt
    15001 tttctttgat tgacgtttac ttggcgcttt ttgtccattg atgttagtaa ttgctacaac
    15061 gttcaaagtg caataaagga ataatggatt atcaaattga attcgtttct cataaaacaa
    15121 ccgttcgaca tcgtcaaaag gagcattcaa tactttagga tattgagcaa cttcaacgca
    15181 ttccaatcct aagttttcta atttctcaac aagtttgtca ctcatcgctg gatcataatt
    15241 cacttgctga atatcatata aattcatgca gtcctctatg tactgcaaaa tctgatcttg
    15301 atcaatcatt tttccgtcgc aaaattcaac gaaaccttgt tcagccaaat cactgtaagg
    15361 aacattatct tctttttctc gaaactctaa atcttcattc ggaataaaat aaagttgctt
    15421 tactttaaga accgctttac catcttcatc ccatgttggg aagtttaaag atacacaggt
    15481 caaatctcgt gtacgtgata aatctaaacc gatataacat ggttcgccac ttaaatcacc
    15541 aagttcttgt gtggtaacta aacatggctc tacttgatcc tgttcaaaga aattatccgc
    15601 gccgttcaca aatacatcca agtgtttcgt tagaaattca gctttggaat gagctgaccg
    15661 ttgcgctgtt ttaaatgccg attctaaagc agataaatcg actgatattc cccagttagg
    15721 attgcacatt tcccagactt ttctatccgt ccaatcataa tttttatttg gctcatagat
    15781 tagaacaaag tttgaatcat tatcatcacg tttcaacact tcttttgctt cgcgatatac
    15841 acgcattcca acagacgacg aacctttccc agcagttgag atattaaaca tcaatggctg
    15901 tggtaaagat atctgagcag acttaaaatt gtcatactgt tccattttct cctgtttatg
    15961 caattcatcg ttcaatacaa aatatggatt ggatccctcg atattatcaa tattcttggt
    16021 ttgaacaata aatttatttg agtaagccat atcttcatga atatagtcat aagtaatact
    16081 agaaacagtt ccctttggcc ccctaaatat tttagttcct tcaagtagga ttggattgtt
    16141 taagattgta actgcaaaag gtttggcagc atattgcgct tgagcaaaat cagatgcgca
    16201 agcatagcaa tcaactgata acgccccttc tccgtacatt gcatatccta atgaaccgac
    16261 agcaatcaat gttttaccat tcttttttgg aatctggaca taagcctcac gagtaacgcg
    16321 tacaatttgg cctttttcat tttctttaac ccaaccataa atccaagaat aaataaattt
    16381 ttcccagggt tctaaaagaa acggttttcc caccatttct ccttttgtgt gaacaataaa
    16441 tgattctacc caatccatca tttcattcgc gcgatctaca tcaaaccaaa tatcttttct
    16501 ttttttccac tgataccagc gatctattgc taaacgaacg gtcttaggat acttcttagg
    16561 tttctttctt acttcttttg caaataaatc agcataattt acaccaggtt caatcatttt
    16621 tcagcacctg ccttcttacg ccatttattt ctgtgcgctg ctagttcatc tactggcttt
    16681 acttctgggc gtgtaatttc ttcatctttt cttgcggtcg atcctccagc aattagtcta
    16741 ccagtttttg ctttgtttgt taatcctaat aaatctagag ctttcatctt cttatctgcc
    16801 caagtttcta cttgttgtgc caatggatgt tttgattgat tagtagctcc tgatttattc
    16861 gtgaattttt gcgtctccgg aaatcctttt tccttccaca ataaatattt gtgttggtaa
    16921 atttcaaaaa tatccaagta tgattcgatt aatggatcaa gagtgatggt gtacaaatca
    16981 gacaaattca ttatttctaa gatacgagct ttttcagcac ttactttttc atcaataatc
    17041 gctttacgtt gcgctttagt cgtcattttt gtatacaccc ccctttgttt tttgaaaaat
    17101 ttgacctaac gatgcgcgtg actccccgct accctatctc cccacgcgaa aaaattttga
    17161 aaatgaatag gggggcttca attgaaatac gaaggaaaaa cttttttgtc ttccgtttcg
    17221 ttttcaacaa ttggatgaca ttttgaacat aatagcatga gattgtttgg atcaagctta
    17281 agcagttcgt tgtctttgat tggtacaatg tgatggacgt gtgctctctt tccaaatatg
    17341 aactgaccac accgctgaca atgaccacct tctctttcat aaataaattg acgcatatct
    17401 ttccatgctt gcgttcgata gaatggtttg ttctcatgat gataaacaga ctttgcttgc
    17461 tgcttcttct tgcgtgatct gcttgatctc ttgtgttcag tacagtagat accctttgct
    17521 attttgttcg tgcatccgtc aaactgacag tatttcattc tacttcacga ataagattta
    17581 tgatatcgcc ttttgcacgg atagcaccag gaatatcaat accatgtttc ttagcatatg
    17641 cacgcaattc ttttgcagtc atattgtcca gttcatccgt atcttcagtg gactgatcat
    17701 tagtagcttc gtctccatca aaattagcag cagtgtttcc atcgctatca agaatttcac
    17761 tgctatgagc tatgagttcg ccattgacag caacaaatga tttgccatca ctcaaaattc
    17821 ctttttcttc ttttacttca aaatttggtt cttgatcttt cggaacaact actgtccttt
    17881 tcttttctga atcccaatac tctgttccac taatggatgt tctaattttg atcattgcca
    17941 ttttaattct ctcctttgta atttgtacgg attacttttg cgcccattcg ctcataccat
    18001 tcaatcgttt cctgcagatt gggtagagtg tgtgacaata aagaaataac taacgtattc
    18061 attccagttc tgtaatcatc attctccact tgcacatgtg catgattacc attccaattt
    18121 tctctgtacg gactattctc tgcttcaatt gctttcttat aaactttagc caaacttgat
    18181 tctacttcaa tactaagtac cgcttcatga aaatcattca tttgtttact ctcctatcag
    18241 atattttgtg gtatgattta taaaaaagat gcgaggtgtt ttaatgcttc aagaagttaa
    18301 tattaagtat cgtgatcgtc aaaatacaga gacaatttat ttgccaaata gttgtccgca
    18361 ttgcggaaat ataatgactc cgtatgtgta ttttggggtt tcatcatata gtgcgttcga
    18421 taatgataga atctttggcg tattagtaca gtgtataaat gatgagtgca acaaatttta
    18481 tagtttacaa tttataaata cgtttaatga agatgaagga tattctgaaa atattttaat
    18541 taaagattat taccgtccgc cgattaaaat agatttacca gaaaatattg aaaaagtttc
    18601 tcctacattt gttgatattt atactcaagc cacacaagct aaatctgagc aattggaaca
    18661 aattgctgga gttggttata gaaaagctct agaatttcta attaaagatt atgcaatatc
    18721 taaaaattca gaggatgaag ataaaataaa aaaaatgtta ttaggtaaaa caattgaaaa
    18781 ttatttgaat gattttccta aactccaaag attagccaaa gctgctactt ggattggtaa
    18841 tgatgaaacg cactatgttc gtagacatac taataaagat gttcatgata tggatgcatt
    18901 tattaaatca gcagcgcaat ttatcgcggc tgattatgat gctgatttag ctgatcaatt
    18961 tataaatgga aataacttta tgccaactac taattaaaag aataaaagag ccacagatac
    19021 caattaataa aacggttggg atatcttggc tctttcatat ttttttgaca ctaatagaat
    19081 atcatgctgc taacaatgtt tacatagcta caatgaccga acatttagtg aacagtgtaa
    19141 ttttagaata aagagagttg ttctttgtac ttctcgctat tgtaaaaatc aatttcgttt
    19201 tcttttttca atcggtcagc ttgtctgtgt tcatactcat ctaaaaagtt caatgtcttt
    19261 ctaatttctg catgtcgctg tctgacatac gatgagctat acccaacaga ttcagcaata
    19321 tcttctaagc tcatttgatc aacatatttc atcttaacaa tttgattatc gataccagaa
    19381 aagctatcta tcagcaataa catttcttct ttttgctcga tcaacaattc caattcattt
    19441 tctatctttt ggatattctc ttctaaagaa gaagttctcg aattcttttc gatgcggacg
    19501 tttgctaaat caccacagac ccatcgattc aattcaagct tacttttatt aagattccac
    19561 tttaagtaaa gaatctgctc atctagttct tggtaatctt ttaaccaatg aaatctcaca
    19621 aacgccaccc cttatggtag aatagtattg cgctgctatc tcgaaagggg tagctttttt
    19681 attttttctt agccgttcca ttctggattg ccttttgttc caaacgtcgt tttttcttct
    19741 taatctttga ttttgttttt cccatacaat tctcctttcc gaaaacacat tttgttcgat
    19801 ttttaatact aaataaaagt tggccagaat ttttcgttcg tctttactca tctttttttg
    19861 aataagatta ctagcataaa aatagttagt accacgaaaa tgaacaggta aatcaattac
    19921 gttcaccttc ttcatgccag ttatcagcca ctagaatctt tgcgttagtg ccgccaccgt
    19981 tttctttttt gtatttttca aatgctttat aagcagctgt ttttgattta tagatttttc
    20041 cggaaccgac tgggaacatc catgtgccct ctgtgatagc taagatgtat actttcattt
    20101 aattcacccc tttaatcccc aacactacat atccggactt ctgcatgtaa tcagtaatat
    20161 aagtgatctc tgctttgtat gagtcaccag tgtaactaaa tgttggtctc gttagtccag
    20221 gtaattcatg tttaatgtat tttttatatt cacgtagtat taactgatca ccaactttga
    20281 agtcacgatc attcttgcgg atctcgaatt gtttgcgtcc gctcgtcact gcttcaaaat
    20341 attctggtag tatttttagt tcgtggatca ttctgtttcc tccttcaaaa ttcgctcatc
    20401 atcaaaaagt gaatctccac aaaatccttc tcccaaatat ttatcgttca tgtataagtc
    20461 aaccaaatat ttgtgatctg cttctcgata ttcattaata ttaacgatct ttattttgta
    20521 aacattacct tcgtcgctca ctatcttgaa ttgttctctg actttcacca ccgttcctcc
    20581 tctttttgaa aaaattttag tgtttttgta agacccccta aatcag
//
LOCUS       DAYYKN010000045        20393 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000045 DAYYKN010000000
VERSION     DAYYKN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.045"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(383..1849)
                     /locus_tag="ACHBB7_001350"
     CDS             complement(383..1849)
                     /locus_tag="ACHBB7_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9209310.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2140..4224)
                     /locus_tag="ACHBB7_001351"
     CDS             complement(2140..4224)
                     /locus_tag="ACHBB7_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9209311.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4366..4488)
                     /locus_tag="ACHBB7_001352"
     CDS             complement(4366..4488)
                     /locus_tag="ACHBB7_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209312.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAGAMIEEKKKE"
     gene            complement(4749..5108)
                     /locus_tag="ACHBB7_001353"
     CDS             complement(4749..5108)
                     /locus_tag="ACHBB7_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM9209313.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5138..5479)
                     /locus_tag="ACHBB7_001354"
     CDS             complement(5138..5479)
                     /locus_tag="ACHBB7_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209314.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5476..6150)
                     /locus_tag="ACHBB7_001355"
     CDS             complement(5476..6150)
                     /locus_tag="ACHBB7_001355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9209315.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7326..8624)
                     /gene="asnS"
                     /locus_tag="ACHBB7_001356"
     CDS             complement(7326..8624)
                     /gene="asnS"
                     /locus_tag="ACHBB7_001356"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM9209316.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8664..9854)
                     /locus_tag="ACHBB7_001357"
     CDS             complement(8664..9854)
                     /locus_tag="ACHBB7_001357"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9209317.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9875..10402)
                     /locus_tag="ACHBB7_001358"
     CDS             complement(9875..10402)
                     /locus_tag="ACHBB7_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM9209318.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10449..13238)
                     /locus_tag="ACHBB7_001359"
     CDS             complement(10449..13238)
                     /locus_tag="ACHBB7_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM9209319.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13385..13585)
                     /locus_tag="ACHBB7_001360"
     CDS             complement(13385..13585)
                     /locus_tag="ACHBB7_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9209320.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14038..14358)
                     /locus_tag="ACHBB7_001361"
     CDS             complement(14038..14358)
                     /locus_tag="ACHBB7_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209321.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14861..15979)
                     /locus_tag="ACHBB7_001362"
     CDS             complement(14861..15979)
                     /locus_tag="ACHBB7_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209322.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16212..16334
                     /locus_tag="ACHBB7_001363"
     CDS             16212..16334
                     /locus_tag="ACHBB7_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209323.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            16450..17757
                     /locus_tag="ACHBB7_001364"
     CDS             16450..17757
                     /locus_tag="ACHBB7_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM9209324.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17878..19077
                     /locus_tag="ACHBB7_001365"
     CDS             17878..19077
                     /locus_tag="ACHBB7_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM9209325.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(19104..20372)
                     /locus_tag="ACHBB7_001366"
     CDS             complement(19104..20372)
                     /locus_tag="ACHBB7_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM9209326.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT         5900 a         4344 c         3559 g         6590 t
ORIGIN      
        1 aatggtgttc aatcagctga ccttcagcat tagctcaaaa agcggcaaaa tatgaggagc
       61 tatttttccg tttgtttctc taatactcag gtcaaatcgc tgattttaca acctcttcct
      121 taaacggtgt tccaaagctt gcatctgcgg taataagccc agccaaacga aaaatatgag
      181 gggctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
      241 gattcacggt gttccagaag tagcttcttc gaaaaacaag ccgaaacttc acaaaaattt
      301 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
      361 tcttgtaaca agtatttcta ttttaaggct gaaccactgt ttctggttca gtgatttctt
      421 tgtttttgtc tcgacgagca attgctggca agatcatacc taataagatc aagacgattg
      481 gtgtgatgat attcgtaaat aattggaacc accatgcttt aggatcagca gtataatcta
      541 cttttggcac cattcctagg atacatgcaa acgcagtgaa ggcaaagcac caagcaccaa
      601 agataaaacc gatttttgga tttttaacaa atttatattc agaattgaat ttcttataag
      661 ctttattcaa catcatatat gccaagaaca cccataggta acgcattggc atgacaactg
      721 agttcaagtt cgtcaaccat tttactaatt cattcatatc tttgatacca aaaatcggca
      781 acaaaataat gattcctact agtatacctg tcagaagata accattcacc aatgtacctt
      841 tacgattacg tttacgtagc cagtttggta caaattctgg atccgcatct gctaacaaga
      901 tttttagtgg tgcatcaatt gaaaaggcta atgcggagat ttgtccaatc atgtttgtca
      961 atgcgtaaat gatcatcaat gagttaccga taccatagta attacctaaa atttgaaaag
     1021 cactgtatgc cccatttgcc atcaagtctt taggaatatt atcactagca aacagcatac
     1081 ccattgcgac tgagcctaat acagcacaaa tccctaccat tgctgctaag aagatcatcc
     1141 ctttaggaaa ttcctttgca ggatttcttg tagagttgac ataaggggat attttttccg
     1201 cccctccaac tgcaaatact agcatcgaaa cggttgtgaa ataactgaaa tcaaatgttg
     1261 gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa ttccatcgtt ggattgatga
     1321 acggtgccgt tacagctaaa agaataaata agatcgacat gacgaacatc gctgttccgg
     1381 ccagtccgcc aatcactttc aaagtagaaa gtccttttgt tgataaatat aaaaaggcaa
     1441 gaaatataat aagtgaaata actgctacca tttgtactga catcgtattt acgatatttc
     1501 catttccttg gactgcccaa ccaccagcaa tcagaattgc ttgaggcttt tgtgccaaat
     1561 aaggaatatg gacgacccag taagtccaag cggcatagta tgccagtcgt ttattagatg
     1621 tcgtattttc gacccatgaa ctgacgcctc ctttactatc cttgaaggct gatcctaatt
     1681 gtccaacgat caatgcgtat ggaataaaat aaatggccaa aatcaatagc caagaggtga
     1741 caactgatac tccttgctga gcatagttat taactacatt ccccatgccc caaaccatgt
     1801 taaatgcaat taacgcaaca gtaaaccagc gtaattgttt gttgttcatt cgttctctcc
     1861 tttatgataa aaatttgtta tccagatgta atcatattca atttttaaca aacttacaag
     1921 acagtgttat tttatgtgtg tattctttta tttaatacac atcgtttttt catatttttt
     1981 ccatcaaaaa aataagttat tttcttttat ttcattgacc ttattgaatt tcaagctatt
     2041 ttgaattttc tgcaaaatta aaaacattag ttattttata atcctatcta ttttctttac
     2101 atttttcatt aagaaaagga tataaaaaaa agaggacgtt cattcgtcct ctttttttac
     2161 atgcaagctt cgcatggcat tcaaaacagc taatactgtc accccaacat ctgcaaatac
     2221 tgcatctccc attgatgcaa aaccaaaagc acctaaggca agtaccaaaa tttttatccc
     2281 aatagcaaag acaatgtttt gtttgacgat tttcaatgtt ttctttgcta aatggattgc
     2341 ttctgcaatc ttcgatggtt gatcattcat gatgactaca tctgctgctt caattgcagc
     2401 atcgcttccc aatccaccca ttgcgattcc tacatctgct cttgctagca ccggtgcatc
     2461 gttgattcca tctccgacaa atgcggtctt tttattgttt ctttgtaaga tttcctctaa
     2521 ccgctgcact ttatcttcag gaagcaattc gctatatatc ttgtccacgc ctacttgctt
     2581 cccgatatga tcagcaattt ttttcgagtc gcccgtcaac atgactgtgt tcttcacacc
     2641 agcctgtttt aattgaacca atgcgtctac ggcatcttct ttcaaggtat ctgcaataac
     2701 aagataacca ctgtaacttt gatcgatcgc tagataaagc aatgtaccga tttcttgact
     2761 taaaggaaaa gagatttcaa atctttccat caatttgtga ttacctgcta atacttgttt
     2821 gccatcaact tcagctaaga tcccttcacc tgcaaattcc tggatattag ttgttggtaa
     2881 aagcggacct gcgtagcctt ctaaaacaga ttgagcaatc ggatgtgtag aaaactgttc
     2941 tgcactcgca atatattgca tgaatttctt tggatcgatt tttgtatcga tcgtctggat
     3001 cgaaaagtca cctttcgtta atgtgccggt tttatcgaat accactgtct cggtttgcgc
     3061 aagcaactcc aagtaatttg aaccttttac taatacaccg attttacttg cgcctccgat
     3121 accgccaaag aaactaagtg gcacggaaat cactaatgca catggacatg agatcactaa
     3181 aaatgtcaat gctcgataga tccattcaga aaacgccgct ccagaaacaa caagtggagg
     3241 taaaacagct aataaaacgg ccaatcctac aacgactggt gtgtaatagc gcgcgaattt
     3301 agtaatgaaa ttttcagctg gtgctttttt actgctggca ttttctacta attctaataa
     3361 tttgctgata gtagaatttt cataagattt ttctacttga actaaaaatg gtttattttt
     3421 attgataaat ccgcttaata ctgtttcacc aacatataca gatttaggga ctgattctcc
     3481 tgtaagtgct gacgtatcga ctaatgattc tccttcgatg atcgttccat ctaacggtac
     3541 acgttcacca ggcttcacaa gtacaaactg accgatctgc acgtcttcag ggttcacggt
     3601 catcaatcct tccgtcgtct gcacgtgagc tgtttctgga cggatcgcca tcaattcgcg
     3661 aatcgatttt cttgaatgac tgactgccaa tccttggaaa tactcgccga tttgataaaa
     3721 tagcatcact gctacggctt ctggatattc accgatcaac atagcgccaa tcgtagcaac
     3781 agacataaga aaattctcat caaagatttt tcctttgacg atattcatca ctgccttctt
     3841 cactacatca tatcctatca gcagataaat caataaatac cctatcaagc gaattggttc
     3901 attaaaatca aataatagca ataaagcgat acctgcaagg gaaaataaaa tccggaacag
     3961 cgtatttctc tctttttcat ttccatgttc agatactttt tccttatcta tttctgttac
     4021 tttgacatct ggttctaatt ccttcactac tttttttgtt tcttgttcaa tcgtagccaa
     4081 atgatcttcc tctgcgtcaa tcgttagttt tccagaagtg aagtttaccg ttgcttcctt
     4141 gactccattg atcttctgaa ctcctttttc gattttcatt gcacagttcg cacaatctaa
     4201 accttctaat ctgtaacttt tcattttaat gacctcctcg cctcaacata tgaacatctc
     4261 ttcataacta attataaatg aatggttatt catatgtcaa ggtatccgaa agaaaaaatg
     4321 aaaaacatct gcatcggaaa atttcttcca tacagatgtt ttggcttatt ctttcttttt
     4381 ctcttcgatc attgcccctg ctcttatcca acccgcactt gccttcttga tcttgacagg
     4441 atagcaacta agtttgaagc ctgttgctgg caatttgtct aaattcatta atttttcgat
     4501 aggacaatat cttttttcaa tccctgcaaa atgtccttcc cagatattcg aagctccact
     4561 acgctttcaa aaatatagaa agagttcttt aatgaaaatt atttcaactt tccataaaca
     4621 gtaatcccat ataaataaaa gacgtaaaag tcacatggga gaacttatac aggaaaacac
     4681 atagagtcag gtgaaataca tgtatcccac ctgactctat gcgttttcta acctttatta
     4741 ttctcttttt atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa
     4801 gattgcttgg caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca
     4861 tgtgcgagag caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac
     4921 atgccagact ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc
     4981 ttaaaacatt gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct
     5041 gctaaccagc taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa
     5101 caactcattg tatttctcct tctattactg tttttgatta aaactgggca aaaattgatt
     5161 ttattcttct ttttttagct tccagcatca agtattctgc aaattttttt tggatttctt
     5221 ccgtattttc ttttgttact aatgtgtgtt ggattagttt tcctgatggt cgctgatcca
     5281 cccgttcttc tgactgttgc acgtccgctt gcgcaaatcc gataatccgt ttttcttttg
     5341 ttacagcaac gagcaatagt ttattcttat catttaggtc agctgccgtt tttgctttta
     5401 cgaatgattc tggatgatca gtaatcagct gattgatttg aggtaaatct tcttttactg
     5461 cccaccgtat ttccatcatt tctcactttt ctttcgctca tttttatctc cactaaaaaa
     5521 atcgactgcg gcaattgcat ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt
     5581 tgggtggacc ccatcttctc ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt
     5641 gaatatgcca tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt tacgaacaat
     5701 ttggatttta gggtctaagt ctttacgcca gccatgacgg tcgtcggtta taggaagtac
     5761 aaacggctcc atcaagatga tgttgcagat ttccgctgtt tgaagttcat ttagtaattt
     5821 tcgataaacg gattcaaatt gctgcgcaga tttctcagtt ccgaatatag cctgatcgct
     5881 agtattatgc caggtatcat tgattccgat caaaatcgat acatagtcag gttgtaaagc
     5941 taaacaatct tcttgccagc gtacttgcag atctgcaatt ttgtctcctc ctatcccacg
     6001 gttcatgcat tcaaatttca tcgttggata ttgggagatt agttttccgg caatcaaaag
     6061 tggataacct ttacctagat cagttgcttc tgctcggtct cttcctgcgt ctgtaatgct
     6121 gtcaccgata aataatatac gttttttcat ttgtcaaaaa ctcctttttg aataaaacga
     6181 tgattttctt ccttatatta tcccataagc cctatcaaga cgaaagagaa aatatatgta
     6241 taaaaaatga caccagccta gtcccttcaa tcaattgaat gaacgctcat caagtgtcta
     6301 gttttttata ctttttttct taacgtgccg gattcccctt gattccttcg ttgtttggtc
     6361 cagagaaatc gatcacacct aagcttccgt caatcagacc actgatgacg ccatcagaac
     6421 atatatttat aggctcatct aagacttcca gtaaatgttc ttttactaat atctcttcta
     6481 gattgcttgg gatagattgg tcataacttt atctggtccg atgatttcaa ggatgaatta
     6541 aacttacata tggatcattg catgctgaaa atacggacct actccatctt cgaatgttac
     6601 tattagtttg ttttcaggta ctagatgctg ctttatttca acagcagaag catcatcaaa
     6661 tcagatttcc atttgaataa ccttctttgt atatgtttga ttgattgcga tttcgttata
     6721 gaaccttgtg aatcaaatag caatttcttt tgctttcttc tcttgtctgc tggaaacttc
     6781 ctttatcccg tattttaaaa aaagaactgc gagagttcgc agttcttttt tttaaataaa
     6841 cgttgctctc cggccaactc ctcggtcaca agtcacaata gtcatcaaac atgaaaaact
     6901 gttttttgaa attactcctt gtgactcgga gctgaacggc aggcgcacaa cctcttgata
     6961 taaacggtgt tcaatcagct gaccctcgat attagctaaa aaaacggtaa aatatgagaa
     7021 gctatttttc cgttttgttt ctctaatact caggtcaagg cactgatttc acaacctctt
     7081 ccttaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg
     7141 gaaagccatt tccacaaaat ttttcttaat actcgaaact aaacagcttt ttcacaacct
     7201 ctccactaaa cggtattcaa aagcttacat ctgcggtaat aagtcccgcc aattcaaaaa
     7261 catgaacagc tgtttttgct tggctgttct aattacttga tgcagaacag ctttttcatc
     7321 acctcttatg gataaattcg gttcaacaaa cgtgggaatg gaatagcttc acgtacatgg
     7381 tcgataccag caatccaagt gactgttcgt tcaaggccta gaccaaaacc agagtgcgga
     7441 acagaaccgt atttacgtag atcaagatac catgaatatt ctttttcatc caaaccgtga
     7501 ttacggattt gttctagcaa gaaatcatag tctgttgccc gttcgcttcc gccaataatt
     7561 tcaccgtaac cttctggtgc gatcatatct gcacaaatta ccacgtcttc tcttgttgga
     7621 tgtggtttca tgtagaatgg cttgatggct tttggatagt ttaggataaa gacaggctgt
     7681 tcgaatgaat tcgcaatgaa cgtttcatga ggtgaaccaa agtcttcgcc ccattcgatg
     7741 tcatcaaatc catttttctt cagtaattcg attgcatcat cgtaagtaat acgagggaat
     7801 ggcaattttg tataagtttt taagacttct ttgtctcgtc ctagtacatc taatgcatat
     7861 tcgcaatgat ctaaaacatt ttgtactaag aatgctacat attgttcctg tacttctaaa
     7921 ctttcttctt gatgagtgaa agccatttct ggttcgatca tccagaattc gatcaaatga
     7981 cgtcgtgttt ttgatttttc agcacggaaa gtcggaccga atgaaaagac ttttccgaaa
     8041 gccatagcag cagcttccat gtataattgg ccactttgag ataaataagc tttttgatca
     8101 aagtaatttg tttcgaatag atccgtagtg ccttctggtg cagaaccagt caagatcggc
     8161 ggatcgattt tgacaaaatg atgttgatta aagaattcat acgttgcacg aatgatttca
     8221 tttctgatct gcataatcgc atgttgtctt gaagaacgca gccataaatg acgatgatcc
     8281 atcaagaaat ctgttccgtg ttcttttggt gtgatcggat aatcgtggct ctcgcttacc
     8341 acttccacat ttgtaattcc gatttcataa ccaaattttg aacgactgtc ttcacggatt
     8401 tctccagtta ctaacacgga tgtttcttgg tgtagatttt tagctgtttg gaaaacttct
     8461 tccgacactt cgctttttac aactacacct tggaaaaagg ctgttccgtc tcttaattgt
     8521 aaaaatgcga tttttccgct tgatcgctta tttgcgaccc aagcgccgat tgttactgtt
     8581 tctcccacat ggtttttcga atcgatgatt tgaatttgtt ccacgtatga ctctcctttt
     8641 atattccttg aatttcttca ggattaattt tgacttttac tctcaataaa ttgcttgatt
     8701 ctgcgtaccg cttcttctaa cgtagcgcga tcagtggcat aactcatgcg gacattttct
     8761 ggtgccccaa agccttcgcc agtcacaagc gctacccctg tttcttctaa aagatcttct
     8821 acccattgtg tgacattttc atacttacac atgcgtagcg tttcttttac attaggaaaa
     8881 agatagaaag ctccttgcgg cttttcaaga gaaacacccg gcagttctgc cacaagcgga
     8941 tataacgtat tcaggcgttc ttcaaaggcg ttccgcattt cttctactgt atcttgctca
     9001 cctttcaatg cttcaacagc tgcatattga ctgactgctg tcggattgct agttgattgg
     9061 gaagcaacag cgatcattcc attgatgatc acaggatttc ccaccgcata accaatgcgc
     9121 cagcctgtca tcgcatatgt tttagacacg ccgttgatga tgatggtctg atttttgatt
     9181 gcttctgaaa tcgttgcaat tggagtaaac tcattgccat tataaactaa acggccataa
     9241 atatcatcag caactatcaa aatatcattt tttactgccc attcaccgat tgcctgcagt
     9301 tcattcttgc tataaatcat tccagtcgga ttcgatggag aattgatgat tacggctttt
     9361 gttttttctg aacgtgcttg ttctagctgt tcaacagtaa ctttccagct attggattct
     9421 tcccctttca caaagacagg aagtccttct gccagtttta cttgctcacc gtaactgacc
     9481 cagtaaggca caggaatgat gacttcatct tgcgggtcaa ggatcgtttg gaaaagggta
     9541 tacaaagcaa atttcgctcc gtctgttacg actgtttgtg aaggttcata gacaagtcca
     9601 tagtttttct tcagataatc aacgattgcc tgtcgaagct ctgggattcc tgctgttggt
     9661 gtataatagc tagctttccc attgcggatt gcttcgattg ctgcttcttg gatgttttct
     9721 ggtgtcgcaa aatccggttc tcccacagtc aaactcagca catctctgcc ttttgctttt
     9781 aatgccttgg cctttgccgc agctgccaaa gtgacagacg gttctaatcg ttttgttctt
     9841 tttgataact ccatcccatt cattccttta ccttttaaat tcccttgatt gtcttgactt
     9901 catctccaga ttcaaagcca atcaggtaat aattcagttt cccatttgtt tcttttccta
     9961 ctacttccca cacaggctga tcatcataca tccctaaagc agctttttcg atttggattt
    10021 caggatgggc cgcagcaatt gtctgttttg cttcgttttc agtcagtccc tttgtttgat
    10081 ccaatacttt tactttttca ccagatttag gaatgataac ggcgatttct tttcctttat
    10141 catttgtacc tgtcagtgaa aagtatgtct tctttcgagt aaaccaataa aaatgatcgg
    10201 tttcctcaag atttgcatat ttctttgcga tttctactgc ttctttcttc gcctgcaccg
    10261 ccggacgact ggaacgcaag taaaaaatcg agctgatcac gatgaccgct aataaaaata
    10321 cgatcaagcc aagtaacacg cgattaatcg ttttttcctt tgtctgtttt ttcttcaagg
    10381 gtcgtcgctc ctttatcttc attccttttc attatagcag attttaatta tgaaaaaaga
    10441 cagctcctct aacttccttc ttcatttttc tttaagaatt catggatatc ctgatagatt
    10501 tcctccatag ctagctcttt gatcggtact tttttcggta atgcatggat gatccgtgcg
    10561 ccatattttg cagtgactag tcttcgatcc aaaataagca atacgccacg atctgtctcc
    10621 gtccttatca agcgacctaa tgcttggcgt aacttcaaag ctgctttagg tacggattcc
    10681 tggtaaaaag gattgatccc ctctgattct aaataagcgt tcctagcttt gaccatcggg
    10741 cgaagcggat tatcgaaggg taaacgtgtg actacaacga tctgcaaagc atctccaggc
    10801 aaatctacac cttcccaaaa gctgtcagcg ccaaacaata cgcttttttc tgacaaaatg
    10861 aagcgtttca aaagcttctc ccggcttcca ccaaaccctt gagctagtat ttctcgtcct
    10921 ttttctagca tagacagatg cattttctga tagaccttct gcaaaacatc atgagaagtg
    10981 aataacacaa gtattggccg atcttcttgt tcgatcagac ctgtaagtgc tgtctctaaa
    11041 taatcgatat atgcttgatt gctgacttga tggacggcag gtgcctcttt tggcacatag
    11101 atccttgcct gtttctcata atcataagga gaggcaatca ctttaagagg tgctgcttca
    11161 atgccaagcc tcttagagag atagtgtcga ttagggccga tcttcagcgt tcctccaata
    11221 taaagaatac gatcatatcg aggataccaa acagtcgacg gcaacatagc cgcttgaaaa
    11281 tcatggagat gaaaaatcat ctgctgcgct gtgttcccag gaacgaacca atggatcaaa
    11341 tgatctcgcc agtcttccag ccattgatcc atcagcaagt attgcttgtc taattgatca
    11401 aataggctat ataaacttgc taaatcgatc cgcatctggt ttgtccagct ttcttggata
    11461 atctcaaaag tcagacgcat ttgtgactgt agctgctgga tctcctgata aaaaagctgc
    11521 aggtgatcaa tggctgtccc agattccagt ggcagctgat ccaacatctc ttttgtcatc
    11581 atgacttctt ctggatactg tccgttaggt tgcttgccgc tcactaaact cattaatcct
    11641 tctgccatac aaatctgtgc ttcgcatagc gcagaaagtt cttctcgata aatcgataac
    11701 cagcgtaagg cttcatgttc tttcggtaaa tagctttgta ttcggtcaaa cagctgattt
    11761 ggttcttcta attgctggat ttgtttatgg aaatagttaa tacttaatcg tttgtcattt
    11821 actttttcag cgatttccgg caaatgatgt acttcatcga tcaacaaaaa tggactagct
    11881 ggaagcaatg gttcttttcg ctgtgtttct tgtactaaaa acgcatgatt gacaatcaaa
    11941 aaattacttt gcctgatttt cttttgcaag aaacgaacaa agtcttgttc gtaaaatgac
    12001 tgatttcttg ctaaaaatcc aatacctctg tgcgcgatat ctgcaaagaa tgaatggttc
    12061 aaatttgtta aatgtaattc atccaaatca ccagtcttcg tttgcgtcag ccaaactaag
    12121 accgccattt gatacaaagc atattgtttt tgttctggcg gttccacaag tgtgccttta
    12181 aaacgctgca aatcaatata gtggcgatag cttttgataa ttacaggttg tatgggctga
    12241 tctagcaatt gattcagcaa tggaatgtct ttttctatga tctgctgctg caaaactagc
    12301 gaaacggtac tgatgacagc gggtttttca ggtgtagcca aataactcaa tggcagcaga
    12361 taaccgattg ttttacccat tcctgtagct gcttcgatca tcaaatcttt tgattcatct
    12421 tttgtgaaat ggtcgtagac cgcattcatc agtctgttct gttcttttcg atacatcagt
    12481 gttttaccaa acattttttc ttttgcttgt ttttttcttg gatatgtccg ctcgccataa
    12541 tggacggatg tgaatagttc cacttctttt ttctgcaaag ctagcccatc gacgatttct
    12601 aatgagggat caagtggctc tggtttttct ttcatttctt caatcacatg ataaatgaaa
    12661 cgactcgtat ccatacctgt gtgtctactt aatcgcgcaa tctgctctaa cgtgatgatt
    12721 ggcaattttc tcatccgttc ttcgatcagc aaaagaagtt gacctgtcac ttcggcatca
    12781 ctatctgcct gatgaggatt ctcatgaata aatccaaggc tttctgacaa atcagctaag
    12841 cgaaagcttg gttccgtcgg caagaaaatt tgtgcaagtt ctactgtatc gatgcctggt
    12901 atcttcaaac taggaagtcc gcatcgcatg agttcatggt tcaaaaaatt ataatcaaaa
    12961 taaatattat gcgcgacaaa tatggtgtct gccaaaagat tgtaaatcgt ttgggcgaca
    13021 tcttcgaaat aaggcgcttt ttggacgcgt tgattcgtga tccctgttaa atgctggatt
    13081 tgttttgaaa tcttccgccc tggatgaata tcgatcgaaa aacgtgaagt gatccgacct
    13141 gattcaatca tgacgcagcc aaattggatg atccgatctt cttttggatt cgtcccagtc
    13201 gtttcgatat caacgactgc ataagttctt ggtttcaaaa tatcttcctc ttctcctgcc
    13261 tctaatgttc ttttgaaatt atactacaaa gtgcagaaaa atactctcat cgctttttaa
    13321 agaaaataga caaaaacgcc cagacacgca aatgtctggg cgtttatttt ttagtatagg
    13381 ttaattaagc tttagtaacg tttgttgctt gaggtccgcg ttggccttct tcaacatcga
    13441 aagttactgc ttggccttct tctaaagttt tgaatccgtc gccttggata gctgagaaat
    13501 gaacgaatac atcgtttccg ttttctgcag tgatgaatcc aaatcctttt tctgagttaa
    13561 accattttac tgtacctgtt tccataataa aaatcctcct tgtgtatcaa cacatatttt
    13621 gcaattactt gaatggtgta tatctggaag atgtttattt gaaacaagct accacagatt
    13681 accgaacaaa aactactcct atagtatatc ataccgttta caaaaaagct agagtttttc
    13741 gacttttttt attaaaaaaa gttttctcag cgattacata aagtgaaaaa ccgaactttt
    13801 ttttaaaata aacgacatat tgttcagttg gtgttttttt gctctgatgt tcttttttaa
    13861 actaaaaaaa ttattctttc tgactactta tcggttctgt ttcaacggtc gaggctgggg
    13921 aagattcttt agtaggtctg atgttctcat ggtatttctg atagcctatg acagttacca
    13981 gaagaacaag gcaaaccaat acgaccaacg agattagttt ttcttttttg ctcatatcta
    14041 tacctcataa ataacaggat gatgtccatc atatttctga gtatgagtaa aagcaaaagc
    14101 tgcagctgta gaatgtaaga aatgccagca actttcataa tcacaggtcg gacagtacca
    14161 aaagtaacca aatcttggct gcaaagaccg ttcttctgtt tcaggaatcg cagaatcaag
    14221 atctggtgtc tgtacataaa catttttgta cagacgtgtg cctgtaatag gaaaccgctg
    14281 tgtttcttca gcaaaaactg gctgacttgt taaacttagg gatagagaca gcaaacacca
    14341 taacagtctt tttttcataa agacctcctt atcgataagt ttttcccaat taaaatcata
    14401 tctcaagaac gcccgttatt cacattttgt tcataaatga tgttttttgt atcataatcg
    14461 tcaaaaagag gtcgtgacca cgtcacaacc tcttttttaa acggtgttcc agaagtagct
    14521 tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa taatttttgt caatctcttc
    14581 ttatttcttg aagctgacca cttctgtcac aacctcttcc ttaaacggtg ttcaaaagct
    14641 tgcatctgcg gtaataagcc caccaaacaa aaaatatgaa gagctatttt tgcttgctgt
    14701 tcttattact tgatgcagaa cagctttttc acaacctctc atacacggtg tttaatcagc
    14761 tgaccttcgg tattagctca aaaaacggca aaatatgtga aactattttt ccgtttgttt
    14821 ctctaatact caggtcaaaa cactgatttc acaacctctt ttaataacga agaatcgctg
    14881 tcagactctt ttcatctggc gcatcttttt gatccaaaat aaacactttt ccgccattaa
    14941 tgatcacatc atctgctaat gcattcaaca cttgacgccg atcatattct gtttcaggat
    15001 cttcaccgaa accatcagct aagtttgacg tagcgataaa taaatgtgac acttttcctt
    15061 ctttcgcagc tggaacgata tcaactaact gatcgatgaa tttcttatct aagagtttat
    15121 tgtattcttt tgattcttga gctgcgagtt cacttgacag cttcttcgcc ccttcttcga
    15181 tatctctaaa ggatagctgc gcaggagaag cagaaatcgc aacgtctgca caataatagg
    15241 gatttttagc aatttttttg aacatcgtct gattttctgg tagagcaaat aaataaatcg
    15301 gccattgttc tggattttcg aaagaatcct tcaagtacgt atcaactgct tggtaatagt
    15361 tgacccaatc gatctctact tcttcatctt ttggatttac gccatggaaa gttactccat
    15421 ctcgggctcc gcctcctcca ttagaagaga agttcaaacc gccacctgtc agttcagatc
    15481 ccaatgcagt aacgacatca accggtgccc cttctggtag agatacttct tttacttgtt
    15541 tgttgctgac ttcatacaat ttcatggagt cgcggttcaa tcccaataaa taataagaat
    15601 agttgaactg cccatttttg ataatagcca gtaaataagg aagatcactt acatagtatt
    15661 gatcgtctac cgggatactc aaacgatgga tataggtttg gtcagcagtt ataatgaccg
    15721 ctacactcgt agttgcacta cgccaaaacg aaggatctcc taacaaattt tcaatttttt
    15781 cttggaacgg ggaccagtca tgttcggaat attttttctc aaaacgtttc tttgcttctt
    15841 tcgcaaaatt tttgaatttg atctgatctt tttccacatc ttgatgggcg acatgcgtat
    15901 tcagcatgat cgtgacgaat ggtcctttag cgccttctga agtcaattgt gacaataatt
    15961 ctttatcctt ttttgccata aacgtacccc tcctaaaaaa atcattcctt tgtttctatc
    16021 taaagtctac cacaacacta tttttccata caataaaacg tatctaaaaa aataaaacgg
    16081 tttctacggt tttaaattta gctatttttt taagttatct tcataatttc ctcaattatt
    16141 tagccccagt cttcaactat tcttcatgaa atgctgataa actaagaaaa agaataaaag
    16201 aggtggatct tatgcatttg attatgattt tcagtttatt aagtattatc agtttatttg
    16261 ctttcagcgc ttcccgctta ttagatgcgg aaacacagcc tattcctgta aaagtacgta
    16321 aaaacgaccg ctaaccttcc ataacgtgcg ttcttatcct aaagtttttt cagaaacatt
    16381 gaaactctct cccctctctg ataaaatttt aatttgtagc gaaacaaatt aattacaagg
    16441 agatcggata tgtccataaa aaaaacacgt ctagtcctat tattggctag tctcattctt
    16501 tgccttgctt cttttctacc agctgttgct gtgcaagctg aagatacttt caaagtaaat
    16561 gcaaaagcag cctttgctgt agatgcgcaa agcggtaaga ttctttatga tcaagatgga
    16621 gaaaaaacca tggggatcgc ttcaatcaca aaaatcatcg gtctttacat cgtccttgat
    16681 caagtaaaag aagggaaact atcatgggat gataaagttt caatttcaga ttatgcagaa
    16741 aatttgagtg tcaccccaga tttatcaaat gttcctttgc ataaagaaaa cacctacacc
    16801 gttaaagaat tattcgactc tgcaatcatc cagtcagcca acgcttcaat ggttgccttg
    16861 gcagaaaaaa tctctggcag cgaagcaaaa ttcactgaac gaatgaaaga acaattgaaa
    16921 gattggggta tcaaagatgc aacgatcgtt aatgcatcag gactgaataa ctcttatctt
    16981 ggcgaaaacc gaccagaagg tactggagaa aacgacgaaa atcaaatgtc tgctcaagat
    17041 gtggctatcg ttgcccgtca tttgatcttg gattttccag aaatactgga tgtatctagc
    17101 actacgacac aaatgtttgg cgaaaacacg caatcaccag tagaaatggt caactggaac
    17161 tggatgctcc caggattcgt gaattataaa gaaggcgtgg acggattaaa aactggtacg
    17221 actgaacttg caggagcctg tttcgtcggt acgatcacta aaaacgatca acgaatcatc
    17281 actgtcgtat tgaatgcaga cggccatgcg gaaaacccaa gtgcccgttt caatgaaaca
    17341 gctcgcttaa tggattattg ctacgacaat tggtcagaaa aagagcttgg aaaagccaat
    17401 gcaagtatcc ctagtctaaa aaccattgat gtaaaagacg gaaaagaatc aacagtgccc
    17461 gttgtgctta aaagtcctgt aaaagtatgg gtacggaacg atatggatac tggaaaatta
    17521 acaatcacac caactcttga caaaaaacaa gtcactgaca atgagatcca agcacctatg
    17581 gataaaggaa caaaaatcgg taccgcaacg atcaccttgg cagaagataa attaggttat
    17641 cttgaagaca acaacgcgcc atccacagaa atcatcacgg ataaaaccgt agaaaaagca
    17701 aatattttcg ttattggctg gcgacgcgtc gcagaatttt tcggtaactt gttttaattg
    17761 aacaaaaaga aaaaagcatc tttccatgtg gaagatgttt ttttgctttt tctagtttaa
    17821 ataacagctt ttttcatcta ttttcgttat actgtataag aaaaaggagt tgaatttatg
    17881 aatctaacca gagatctgat ttttttggtt ctctatctta ttggggtatt ctccatcgtt
    17941 tattggcgaa aacagaaaaa ggatccttcc ttgttctttt acagcagtac atggacgttt
    18001 gcggtaatct tgacttattt tgtcgtgttg gaaatttttt attcgacctt ttattttttg
    18061 attcccttag tgtttttcag tatctttgcc ttttcctatt tttcagaaaa gcgaaggctg
    18121 ttgaatggtc ttttattcaa tattttcctc attagttttg gtatctactt gtttgtcttg
    18181 ctatacgaaa cacaaaatat ttttcttggc ggactgattg ccttgatcac gatcccgtta
    18241 ctgcttgtct ttctattcgg catttatgga ttgatcgttt ttcttttttg gaacggtgtc
    18301 acagttttac gtagagagtc gcattcatta gctaatcttt taacgttgat actagccatt
    18361 tttcttacat tgtttcttgt ttttgacttt ttcttgttaa aatatttacc acaatggata
    18421 aatgctttgt ttttctgcat cccattgatc ttgatctatc tatttatcgt attctacaac
    18481 tttttgactg tctcattctt gtatcagttc aaccgccctc gatacaacca agacttcatc
    18541 gttgtgttag gcgccgggtt gatcaatgga gaaacagttt ctcctcttct tgccaagcga
    18601 atcaataagg cgattgcttt ttattgggca caaagccgtg cgacattaaa tccgcctatc
    18661 ctattaatgt cgggtggaca gggagcagac gaaaaagtac cagaagcaat ggcgatgaaa
    18721 caatatgcca tggaacaagg aattcctgaa agagatattc ttgtcgaaac caattcgaca
    18781 accactttgg agaatatgcg ctattcaaag gaaatcatgg atcagcaaat gaaaggtccc
    18841 tatcgcgcga tcttcagttc caacaattac cacattttca gagctggtct ctatgcacga
    18901 caagcgaaac tgaaggcaaa tggcattggg gcaaaaacag ctttctatta cttgcctaac
    18961 gcgtttttaa gagaatacat cgccatcttg atgatgaaca aaaaaagaca tatgatcatt
    19021 tgcggattgc ttttcgcagg ttccgcattc ttatcactga tcactttact tttctaaaaa
    19081 aacaaggtgt gacaaaacat atgtcacacc ttgttttttt ctttcattct cttaactatt
    19141 ggcaaaaaga gtatcccttt tctgaccgct acttgctgag cagaataaat accgctgttg
    19201 cctgaacaca taaagctgat caagcagatc ataaagaagt atactgccgc ttgtgaaccg
    19261 aacaattcga tccccatcac aaagcaggca atcggtgtat ttgtcgcacc agaaaatact
    19321 ccgataaagc ccaatcctgc aagaaaagga acagaaacat ggagcaaagg cgctagtgct
    19381 gcaccaagcg ttgcaccaat ttcaaacaat ggtgtcactt ctcccccttg atatcccgcg
    19441 cctaaagaca aaactgtgaa gaataacttc ccaataaaat catagacatg tgcctgtcca
    19501 ttaaatgatt cctgcagtaa tggaagactc aatccgagat aacgttgtgt ttgcaaaatc
    19561 aatgctgcgg ctacaactac agccccacct atcgtatttc gccaaacagg atgaggaatc
    19621 cattgtgcat atcgcgcttt taaaaagacg atcgaacggc tgaataccca accaatcaaa
    19681 ccaaaagcaa tcccagcaac aaaaactttg aagaacagca acatactcca ttcaggaatc
    19741 acacccattt ggtaatgtag atgagaaaca ccatagcttt ctgtcataaa atttgcaaac
    19801 agtgcagcaa agaagctagg gaagatcgct tctgttcgga ctttcccgat tgccaacact
    19861 tctaatccaa ataatgttcc agctaaaggt gtgccaaata cagaactaaa acccgcactg
    19921 attccactga taatcaagat ttcccgttct gatttatcca atcggaacaa acgtgctgta
    19981 ttatctgcta acgctccccc catttggact gctgttcctt cacggccaac agatcctcca
    20041 aataaatggg tagttatggt tccaaacagt gtaagtggaa taagtcgcaa cgggattttt
    20101 tcttctccac catttccttg atcgatcact aagttgtttc cacgactaga aagaccgccg
    20161 tgatacgcat agagataagc aaaaagcgca ccactaactg gtagtagata aaggagccaa
    20221 ggatgtgata gtcggatatc tgtcacaact gtcaggctat ttaagaaaaa agcagataag
    20281 ctccccatga atataccgag aatcgctgtg atcaccagcc acttcagcat gtacactaaa
    20341 atgcgtactg gactcggtaa ttgtttttcc ataaaaaatt cctcctcaat aaa
//
LOCUS       DAYYKN010000046        20181 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000046 DAYYKN010000000
VERSION     DAYYKN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.046"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="ACHBB7_001367"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="ACHBB7_001367"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM9209327.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="ACHBB7_001368"
     CDS             1011..2258
                     /locus_tag="ACHBB7_001368"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM9209328.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="ACHBB7_001369"
     CDS             2334..2678
                     /locus_tag="ACHBB7_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209329.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="ACHBB7_001370"
     CDS             complement(2795..3121)
                     /locus_tag="ACHBB7_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209330.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4696)
                     /locus_tag="ACHBB7_001371"
     CDS             complement(3494..4696)
                     /locus_tag="ACHBB7_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9209331.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4813..5328
                     /locus_tag="ACHBB7_001372"
     CDS             4813..5328
                     /locus_tag="ACHBB7_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9209332.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5527..5973)
                     /locus_tag="ACHBB7_001373"
     CDS             complement(5527..5973)
                     /locus_tag="ACHBB7_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM9209333.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="ACHBB7_001374"
     CDS             complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="ACHBB7_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM9209334.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7852..8481)
                     /locus_tag="ACHBB7_001375"
     CDS             complement(7852..8481)
                     /locus_tag="ACHBB7_001375"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9209335.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8465..8929)
                     /locus_tag="ACHBB7_001376"
     CDS             complement(8465..8929)
                     /locus_tag="ACHBB7_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9209336.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9416..10684)
                     /locus_tag="ACHBB7_001377"
     CDS             complement(9416..10684)
                     /locus_tag="ACHBB7_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9209337.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10915..11652)
                     /locus_tag="ACHBB7_001378"
     CDS             complement(10915..11652)
                     /locus_tag="ACHBB7_001378"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9209338.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11719..12288)
                     /locus_tag="ACHBB7_001379"
     CDS             complement(11719..12288)
                     /locus_tag="ACHBB7_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM9209339.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12416..13288
                     /locus_tag="ACHBB7_001380"
     CDS             12416..13288
                     /locus_tag="ACHBB7_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9209340.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13630..14502
                     /locus_tag="ACHBB7_001381"
     CDS             13630..14502
                     /locus_tag="ACHBB7_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9209341.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14499..16457
                     /gene="nagE"
                     /locus_tag="ACHBB7_001382"
     CDS             14499..16457
                     /gene="nagE"
                     /locus_tag="ACHBB7_001382"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM9209342.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16708..17559)
                     /locus_tag="ACHBB7_001383"
     CDS             complement(16708..17559)
                     /locus_tag="ACHBB7_001383"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9209343.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17682..18149
                     /locus_tag="ACHBB7_001384"
     CDS             17682..18149
                     /locus_tag="ACHBB7_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM9209344.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18412..18894)
                     /locus_tag="ACHBB7_001385"
     CDS             complement(18412..18894)
                     /locus_tag="ACHBB7_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9209345.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18994..19857)
                     /locus_tag="ACHBB7_001386"
     CDS             complement(18994..19857)
                     /locus_tag="ACHBB7_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9209346.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6067 a         4156 c         3662 g         6296 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgacta caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
     4441 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
     4501 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
     4561 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
     4621 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
     4681 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgta
     4741 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
     4801 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
     4861 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
     4921 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
     4981 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
     5041 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
     5101 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
     5161 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
     5221 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
     5281 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
     5341 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
     5401 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
     5461 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
     5521 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
     5581 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
     5641 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
     5701 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
     5761 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
     5821 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
     5881 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
     5941 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
     6001 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
     6061 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
     6121 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
     6181 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
     6241 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
     6301 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
     6361 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
     6421 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
     6481 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
     6541 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
     6601 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
     6661 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
     6721 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg
     6781 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
     6841 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
     6901 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
     6961 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
     7021 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
     7081 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
     7141 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
     7201 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
     7261 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
     7321 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
     7381 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
     7441 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
     7501 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
     7561 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
     7621 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
     7681 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
     7741 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
     7801 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
     7861 cgtagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
     7921 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
     7981 ctgatctgct tcgactccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
     8041 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
     8101 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
     8161 catcttccct gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat
     8221 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
     8281 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg
     8341 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
     8401 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
     8461 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
     8521 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
     8581 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct
     8641 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
     8701 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
     8761 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
     8821 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
     8881 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
     8941 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
     9001 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
     9061 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
     9121 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
     9181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
     9241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
     9301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
     9361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
     9421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
     9481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
     9541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
     9601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
     9661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
     9721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
     9781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
     9841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
     9901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
     9961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    10021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    10081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    10141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    10201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    10261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    10321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    10381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    10441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    10501 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
    10561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    10621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    10681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    10741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgata aatttgcttt
    10801 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    10861 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
    10921 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
    10981 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
    11041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    11101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    11161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    11221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    11281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    11341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    11401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    11461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    11521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    11581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    11641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    11701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    11761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    11821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    11881 tttcttgatg tggattggct ttactaacaa gcctcttcgt tcttttggat cggttagatg
    11941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    12001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    12061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    12121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    12181 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
    12241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    12301 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
    12361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    12421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    12481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    12541 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
    12601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    12661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    12721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    12781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    12841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    12901 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
    12961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    13021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    13081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    13141 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
    13201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    13261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    13321 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
    13381 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    13441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    13501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    13561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    13621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    13681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    13741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    13801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    13861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    13921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    13981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    14041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    14101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    14161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    14221 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    14281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    14341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    14401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    14461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    14521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    14581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    14641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    14701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    14761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    14821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    14881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    14941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    15001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    15061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    15121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    15181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    15241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    15301 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
    15361 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    15421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    15481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    15541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    15601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    15661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    15721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    15781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    15841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    15901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
    15961 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
    16021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    16081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    16141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    16201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    16261 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    16321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    16381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    16441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    16501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    16561 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
    16621 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
    16681 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
    16741 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
    16801 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
    16861 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
    16921 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
    16981 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
    17041 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
    17101 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
    17161 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
    17221 tacactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
    17281 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
    17341 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
    17401 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
    17461 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
    17521 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
    17581 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
    17641 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
    17701 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
    17761 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
    17821 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
    17881 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
    17941 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
    18001 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
    18061 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
    18121 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
    18181 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
    18241 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
    18301 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
    18361 tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt
    18421 attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac
    18481 tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa
    18541 ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac
    18601 aagatacgtt ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt
    18661 ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata
    18721 atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc
    18781 aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac
    18841 accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc
    18901 caccctttct attatattaa gttcatctta cataaaatta aagggaaatg acacgattac
    18961 gctctttttt ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca
    19021 aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc
    19081 ttcgactaac ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc
    19141 ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat
    19201 agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg
    19261 catcaaactg cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac
    19321 ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt
    19381 ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc
    19441 cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc
    19501 tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctgctgat
    19561 ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc
    19621 atatccatat ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc
    19681 caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa
    19741 gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag
    19801 tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt
    19861 tatcttcctc cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa
    19921 tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag
    19981 cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa
    20041 taaattttgg ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct
    20101 tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga
    20161 acgctgcttg gatttttctt t
//
LOCUS       DAYYKN010000047        20026 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000047 DAYYKN010000000
VERSION     DAYYKN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.047"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(540..1565)
                     /locus_tag="ACHBB7_001387"
     CDS             complement(540..1565)
                     /locus_tag="ACHBB7_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9209347.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1840..3525
                     /locus_tag="ACHBB7_001388"
     CDS             1840..3525
                     /locus_tag="ACHBB7_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM9209348.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3539..4822
                     /locus_tag="ACHBB7_001389"
     CDS             3539..4822
                     /locus_tag="ACHBB7_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9209349.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4892..5761
                     /locus_tag="ACHBB7_001390"
     CDS             4892..5761
                     /locus_tag="ACHBB7_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9209350.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5758..6594
                     /locus_tag="ACHBB7_001391"
     CDS             5758..6594
                     /locus_tag="ACHBB7_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9209351.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6620..7687
                     /locus_tag="ACHBB7_001392"
     CDS             6620..7687
                     /locus_tag="ACHBB7_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM9209352.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7684..8511
                     /locus_tag="ACHBB7_001393"
     CDS             7684..8511
                     /locus_tag="ACHBB7_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9209353.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8556..9581
                     /locus_tag="ACHBB7_001394"
     CDS             8556..9581
                     /locus_tag="ACHBB7_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9209354.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9578..11761
                     /locus_tag="ACHBB7_001395"
     CDS             9578..11761
                     /locus_tag="ACHBB7_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM9209355.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11761..12889
                     /locus_tag="ACHBB7_001396"
                     /pseudo
     CDS             11761..12889
                     /locus_tag="ACHBB7_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            12886..13563
                     /gene="pgmB"
                     /locus_tag="ACHBB7_001397"
     CDS             12886..13563
                     /gene="pgmB"
                     /locus_tag="ACHBB7_001397"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM9209356.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13647..14174
                     /gene="msrA"
                     /locus_tag="ACHBB7_001398"
     CDS             13647..14174
                     /gene="msrA"
                     /locus_tag="ACHBB7_001398"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9209357.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14375..15061)
                     /locus_tag="ACHBB7_001399"
     CDS             complement(14375..15061)
                     /locus_tag="ACHBB7_001399"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM9209358.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15141..16679
                     /locus_tag="ACHBB7_001400"
     CDS             15141..16679
                     /locus_tag="ACHBB7_001400"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM9209359.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16799..17266
                     /locus_tag="ACHBB7_001401"
     CDS             16799..17266
                     /locus_tag="ACHBB7_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM9209360.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17800..18228
                     /gene="rplM"
                     /locus_tag="ACHBB7_001402"
     CDS             17800..18228
                     /gene="rplM"
                     /locus_tag="ACHBB7_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM9209361.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLAGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18244..18636
                     /gene="rpsI"
                     /locus_tag="ACHBB7_001403"
     CDS             18244..18636
                     /gene="rpsI"
                     /locus_tag="ACHBB7_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM9209362.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18725..19951)
                     /locus_tag="ACHBB7_001404"
     CDS             complement(18725..19951)
                     /locus_tag="ACHBB7_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9209363.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
BASE COUNT         6449 a         3621 c         4232 g         5724 t
ORIGIN      
        1 tctaaaataa tttgtctaac tagaagaaaa gtgggaaagg atcttaacaa catcggttcg
       61 tatccaatta aggtatcgga acgatcatct tccaagagaa gtcaatcaac aaaagtaaag
      121 atagatcatt ttttgaatgc aaaataaatt gatgggaaga tagagcggtt atttttaata
      181 ttttgctagc tattattgaa taattcctcg aataaaacaa aaccagtaga catgtctgtt
      241 attctattta tgttttagag ataagtatcc ccaaataaca tggattgaaa taagggtaaa
      301 tgtgtttaaa aaaacgaact ataaaaactt atacacaacg tggttaaaaa tagttttttc
      361 ttccctgtgg aaaaaatagt ttaacacaaa cattatagtt atccacagct atctaaaaca
      421 gatggaaaat aagaaaaatt cgctaaaata ccgggagggg gtaacgaggg atgagtgatt
      481 gaaaaaagaa cagagtaact gacttatcct cagtgtttct gttcttttat aattctgttc
      541 tacatttctt gttgttggat cgaatctgtg agagtaatct cggttgaaag aatgattctt
      601 ttttcttcag ggtattggtg gtggattagt tggaataaaa gttctgtggc tttgattcct
      661 aactgatttg tcggaatgtc tatccgagtc aactgtggcg aaaataggga ggacaataag
      721 ggatcactga atcctgcgat cgaaagatca tcaggtatct gctttccaga ttgttctgcc
      781 actttgtaaa tactcaatga ttttaatgca tcggtagaaa aaatcgcagt tggtctttct
      841 cgtagcttca ttaaatcatt cattgctgcc atgctatctt ctaagcgaaa gccgccgtct
      901 ataatccatt cattacggat atctaggtgg tgatcaaata agcattgacg gtaaccattg
      961 agacgatcat tggatacata aacttctgtc ggagcatgga gacagccgat atgacggtgt
     1021 ccattcttga tcagtgtttc caccaaatcg taactatcct ggaaattatc cgtgtccact
     1081 gagtagatgt tcgtgtagtc gtttccgact tttccgatta ccacaatagg aatattgtat
     1141 tgatccaatt caaaaagaag tgtttcatcc atcgtagagc ttaaaataat aattcctcgg
     1201 atcatttttt cttggatctt tttcgcgcat ttttctagct cttcttctgg tgtttttgcc
     1261 gtttgtagaa tcaagtcgaa gttattttgt tcagaaacgg cagaaatgga ttgaataaca
     1321 tctgaaaaaa aaggattgcc ataggtcgta tgagaagtac gatgagataa tgctagaatc
     1381 gcgtcaaaac catttcctga cagtcctctt gcaattttgc tcggttcata attcaattct
     1441 ttgattgctt gaagtacact tgcttttgct ttatcagaga tatttggctg gttatttaat
     1501 actctggaga cagttgattt tgagacatta gcttttttcg caatgtcata aatagtaggt
     1561 ttcaatttta tatgcacctc ctttttggga gcgttttaga ataattccat agtacaatag
     1621 acattctttt ttgtctatga ttttgaaaat ttaggaatag cttgcatgat catgagagac
     1681 catatttttt taagaagcta ttaattattg atacataagc tttcttattt gtatttttgt
     1741 gtatatcaaa attatttaaa aaaatgctat tgacagtaag cgttttccat aatatgatca
     1801 aatcgttggg aacgctccca aaaaagaaag gggaatccga tgtatacaaa acttttacaa
     1861 gcgatttatc gcgactcaga tttgtctgag aaaaccgcct ctatgattga acaaaaagtc
     1921 agtgtttatc agcagaagaa aggccaggca ggcaaactgc attgggacga agaagatatt
     1981 ttcttgatta cttatgggga tcaatttttt gaagagcagc aaccaacttt gcgtacattt
     2041 aagaaatttt atcaaaagta tttggcagat acatttagag ttgtacattt cttgccattt
     2101 ttcccatata catctgatga tggtttctcc gttgtaaatt atcaggcaat caacgaccgt
     2161 ttgggcacat gggaagatat agaagaaatg agaagcgaaa cacggttaat gttcgacttt
     2221 gtctgcaatc atatgtctgc cgaaagtact tggtttcagg aatatttaaa cttgaatgat
     2281 acgtacaaag atttttttat tgatttgcca ctggatacag atacaagcag cgtgactaga
     2341 ccaaggacca gcccgttact tacaacgttc attgacaaaa acgggaaaga aaaacacgta
     2401 tggacgactt ttagcgaaga tcaagtagac ttgaatttta aaaatccaga agttttattg
     2461 aaaatgatcg atattctttt gttttactta gaaaaaggcg cttcatttat tcgcttggac
     2521 gctgtgggat ttttatggaa agaactagga acttcttgta tacacctgcc agaaacgcat
     2581 aaaatcatcc aattattccg tgcaatcgct gatgaagcag ctccaggaac ggtattgatc
     2641 actgagacaa atgtgccaca caaagataat atcagttatt ttgggaatgg cgagaatgaa
     2701 gcacagatgg tctatcagtt ttcgctacca cctttgaccc ttcaagcgat tcgttcgggt
     2761 aatacaagta agttgaaaga atggttaaaa accatcgatt tcgcatcgaa aaaaacaact
     2821 ttttttaatt ttttagcctc ccatgacggt atcggattga atccggtacg aggtattctg
     2881 ccggaaaaaa cgatccttga attagtggaa cagctgaaga aagagggtgc ccgagtaaac
     2941 tacaagaaaa acccagatgg aacgaccagt ccatatgaga taaacgctac gtatctagat
     3001 gcgttaagtt ctccaacaga tacgacggaa tggagaagca aacgcttttt agctgctcat
     3061 tcatttttgg taacgttacc aggcgtgccg gcaatttata ttcaaagtgt gctgggaagt
     3121 cgaaatgatt acaaaggcgt agaaaaaaca ggagagaatc gaaaaatcaa tcggcaaaaa
     3181 tttttgttag cagatattga gaaagagttg cagacagatc cattaagaac agcagtatat
     3241 ggtgaattga cccagatgct atctattcga aaaaaagaaa aagccttcca tccgaatagt
     3301 ttgatttact tactggactc agcagattct tttatcagtt ttattcgcga agcgaaggaa
     3361 gagaaagtct tagttatcca taatttttcc aatcaatcag caggttatct tttaccaaaa
     3421 ggagattacc tagacctgtt ttctggacaa attatccatt gcagtcaaga acgaaagttg
     3481 atattccagc cgtatgaata tatgtggctg aaaataataa aataagaggt ggaaaaaaat
     3541 gaaaaaaatt attgttacta cctgtctgct agctactgga tttcttctta ctgcttgtgg
     3601 tgaaagtcaa agctctaacg aatcacaaca gacaacgatt caattcatgc actcttctgt
     3661 cgaacaggaa cgattaagtg tgatcaatga actggttgct gattttgaaa aagaaaatcc
     3721 agacatcaaa atcaagcaag tgccggtaga agaatcagcg ttcaatacaa aaatcgttac
     3781 gttagctcgt tccggtaaat taccagaagt aatggaagtc agtcaggatt ttgctaaagt
     3841 gatggataaa gatgaactga ttgatcagac ggcagtccaa aacgttatcg aggaagctgg
     3901 agaagataat tattatgacg gagccaaaaa ccttgttcgt tcagaagatg gaaaaagcta
     3961 tattgctgca ccaattagcg gctgggttca aggtatttgg tatgacaaag aaaaattatc
     4021 agaagctggt ttttcagaac cagaaaattg ggatgatatt ttaaaaattg ctcaacattt
     4081 cacagataaa gagaataaac aatatggaat tgctatgcct actgctgaag gaacaatgag
     4141 tgagcaagcg ttctctcaat ttgctttgtc aaatggagca aatgttttgg atgatgaagg
     4201 cgaggtaacg atcgatacgg aaaagatgag agaagcattg tcgttttatc aaaacttatc
     4261 ccaatacacg atgccaggct ctaatgatgt gacagaaatc aaagatgctt tcatgaatgg
     4321 aacagcacca atggcaatct attcaactta cattttgcct tctgtttatg aagcggggaa
     4381 ctcagaaaat atcggttttg ctatcccaac acaaaaagat caagctgtct atggcacagt
     4441 atctggattg acgatttctt ctggtctaga cgagaaacaa aaagaggctg ctgaagcatt
     4501 cacagctttc ttatctcaac cgaaaaacat ggaaaagtgg gtcttgatgt ctcctggtgg
     4561 tgctcaacca gtgaataaac aagtagtcga actggatggt tacaaagaaa atgaagtcat
     4621 caagtctttt ggcgagctgc catctgagat tgctagtgct tttgatgaag tacaagtctt
     4681 tggactagtc ggggaaaaga atttcacgaa aatgggagat atcacaagct caggtgtgat
     4741 cggaaaagca gtgaaccaag taactgtcgg aaacgaagat gtcgatcaag ctttagctga
     4801 cgcacaaaaa aatacgaaat aacaagaagt tctgccaatg gtgttcgaaa aaagtagata
     4861 gctattggca gattatcagg gggaggtttc aatgaatcgt ccaaggaaac attctgactt
     4921 aaaattagcc atgggacttt tagcaccaag catgctttta ttagctgttt tagtgctgat
     4981 tccaatgctt tccaatattc agatcagttt tttcgatatg ccgctgaatc ctaactcaga
     5041 cgccgttttt atcggattaa gcaattatct tgaaatttta agtgatagtg aattttatcg
     5101 ttctttaggt ttaacagttg cttacacatt cttagttgta gcaggcagta cagctttagg
     5161 attagccgta gctattttgc ttaaccgcaa gttcagattt aggaaaacgg tgcgttcact
     5221 gattttatta agttatgtta cgccatctat ttctttgatt tttgcttgga aatatatgtt
     5281 caataatagt tatggaatcg tcaatgatct gttagtagat aggttgcagc ttttcaacga
     5341 agcaccgctg tggttcgacc agccagttgc tagcttcttt atggtcgttt tgtttgctat
     5401 ctggagatac tatccgtatg cgtttatttc atttttggct attttgcaaa cagtcgacaa
     5461 aacgctttat gaagcagctg aaatggacgg tgcaaatgct tggcagaagt tcaagatcgt
     5521 gacactgccg gcaatcaaac cagtactagc aactgtgatt gttttacgat ctatctggat
     5581 gttctatatg tttacagatg tctatctact gacgaataaa gtcaatatct taggcgttta
     5641 tttgtatcag acagcctttg cgttcaatga tcttggaaaa gcagcagcta tatcgattct
     5701 attgttcatc attattgcag cggtcgtctt gctctcacga aagaaggtgg acttgaaatg
     5761 aaaaataaaa agagcaggaa aatcttattt tacgcgacga ttttgttgct attagctgtc
     5821 aatttgtttc catttttgat catgctgatg acatcattca aggattcaca agaagcaatt
     5881 gcaacgacac aaactatttt tccgaaagaa tggacttttg aacattatca ggatattttc
     5941 aatgctgaga tttttccatt catcatgtat ttcaaaaata gtttagtgat ctccttgata
     6001 gcctccgtaa ctgcagtagt gattgggatc agcggggcat acgctttgtc aaaactacgc
     6061 ttcaaagctc gctcgaaaat caatgctagc ttttacaccg tttatatgtt ttccggtatt
     6121 ttgatcgttg ttccattatt taaaatcatt tccggtctag gtttgtatga taccaataca
     6181 gcactgatta tcacgatggt cgttcaaaca cttcctactg cgatattcat gctaaaaagt
     6241 tattttgaca cgattccgac tgatattgaa gaagcagcaa tgatcgatgg tcttagccgg
     6301 atccagatca ttattcggat cattatacca ttggctattt ctggaatcat ttctgtcttc
     6361 gtttattgct ttatggtttc gtggaatgat tatttatttg cttcgatttt cctgtcgacc
     6421 tccgaaaaat ttactttacc aatcggtttg aacacattat tcagtacacc agactacatt
     6481 tggggacgaa tgatggccgc ttcattggtg accgctttac cagtcatcat tatgtatgcg
     6541 ttatctgaac gatttattaa aggtgattta gtcgaaggcg gcgtaaaagg ataaaaagaa
     6601 aagaaagagg aaagaaaaga tgaaaaaatt agttgcatta gaaccaagaa tagccggatt
     6661 caaagattac gaagaccgtg agatcgaaaa gaacgaagta cgtatccgag taattttcgc
     6721 tactccaaaa ccaggaacag aaattgtcga ctttagagga ttaagtccat ttatcgatga
     6781 agaattttca gaagaatggc gcctattctt gccaagagaa gaaggaagcc aacgcggtgt
     6841 ggtatttggc gaatttcaat taggaaatat gattgtcggt acgatcgaag aaacgggcag
     6901 tttggtgaca gagtatcaaa aaggagagtt agtctgcact tatggaccca ttcgtgaaac
     6961 gctgattaca aacggtgttg ataattataa actacgtaaa cttgatcggt tggatcaatg
     7021 gaaaaatgca gtatgttacg atcctgctca attcgcttta ggtggtatcc gggaagcaca
     7081 tgttcgagca ggtgatgctg tagcaatctt cggtttaggc gctatcgggc aaattgcgat
     7141 tcaattagct aaaaaagcag gtgctactac catcgttggg atcgatccca tagcgcagcg
     7201 tagagaatta gcagaaaaat acggtgccga ttacacgcta gatcctatga aagcagatgt
     7261 cggttttcgt ctaaaggaat tgacagataa acgtggagta gattcagtta ttgaaacgag
     7321 tggcagtgct gccgcattac aagcttcttt aagagggatc ggctatggcg ggatcatttc
     7381 ttatgttgcc tttgctaaac cttttccagc tgggtttaat cttggtcgcg aagcacattt
     7441 caataatgca aaaatcgtct ttgctcgagc atctagtgaa ccaaacccag attacccaag
     7501 atggaaccgt aaacgaatcg aggacacatg ttgggaatta ttgatgaacg gttacttgga
     7561 ttgtgaagaa atcattgatc cagttgtgcc atttgcagaa agcgcagaag cttacatgcg
     7621 ttatgtagac cagcacccag aagaaagtat caaaatgggc gttcaagtcc gaggaggaga
     7681 acaatgaaat tcggtacgca agatcaagca tttttcccaa gagacatttt agaaaaattt
     7741 agatttatca aagactgtgg atttgaagaa tacgaaatcg acggtaagtt gttaatagaa
     7801 aatatcgaca cagtcaaaca ggcaatcaaa gaaacaggga tcccagtagt gacagcttgt
     7861 aacggctacg atgggtggat cggtgacttt atcgaagaac ggcgtcttaa tggtgtacgt
     7921 caaattgctg agattttgcg tgcactaagc gaggttggag gcaaaggaat cgttatccca
     7981 gcagcttggg gaatgttcac ttatcgatta ccgccgatgg tttcaccccg aagcaaagaa
     8041 ggagactata aagcagtcag cgaatcactt cgttatttag ataaagtagc cgaagaaaca
     8101 ggtacggtta tttatttgga gcctttgaac cgataccaag accatatgat caatttgtta
     8161 gcggatgcgc gtcattacat tgactctaat caattgaaac atgtgcagat catcgctgat
     8221 ttttatcata tgaatattga agaagatgat atttcaaaag cattgactac gcaaaaagag
     8281 tatgtgggtc atatccattt agcagataat catcgctacc aaccaggatc tggcgcaatt
     8341 gattttactc gccattttca aacgctactt caaaatgagt atgaaggtta tatggtatat
     8401 gaatgtcgtt tgcgaggaga aaatgaagtg aaaatttatc aggaatcatt gcaatacttg
     8461 aaaaactgtc tggaaacagc gcgtcattta aatgatttag agaaagaata gagaaagaat
     8521 aacaataagt aaataagcaa aggagagaga aataaatgaa gaaagtagca atcataggcg
     8581 ctggacaagt agcagaaaaa gtacatgctg cttattatca aaaaagatca gatgtcttag
     8641 aattggccgc tgtggttgat ccagtcagcc agcgagcaga agacttttgc cgacgtacag
     8701 gattttctca tgcgtataca tcagttacac aaatgctgaa tgagatttct cccgatatcg
     8761 tgagtgtctg tacagccaat cgctttcact tcgaacatgt attggctgcc ctcgaagcta
     8821 atgcttcagt gatgtgcgag aaaccaccag ctatgacagc tgatcaagca agaaagatgg
     8881 cagagacagc cgataaaaac gggcagattt tggcttatgg attccagcat cgtttttctg
     8941 acgaagcaac aatgatcaag aatcatcttg atgttctcgg ggatatttat tacgtagaag
     9001 cgaatgcgct tcgtcggtct ggtgttccag gctggggaac gtttatcgac aaagaacttc
     9061 aaggaggcgg gcctttgatc gattatggca ttcacatgct tgatacagcg atgtatatta
     9121 ctggatttcc taaagtaaaa agcgttcggg cgtacagctt ccaaaaaatt ggtacgaaaa
     9181 aaaattcagg gaaattcggg aaatgggatc caagcaaatt cacagtagaa gatgcaattt
     9241 ttggcacact ggaattagaa aacggcaaga tcatccaact gaatacttcg tttgctttga
     9301 atatcagaga agacaaactg aatgtggctt tttgtggcga tcaggcaggt gctaatctgt
     9361 atccaggcga actattcgaa gatgtagatg gaaagctggt ttccttacaa aagaccacgc
     9421 cagttaatga cccacatgaa ctaagtatgg ctcattttgt gaatcgggcg ctcggtgacc
     9481 agactcagat gattgcagat ggctggcaag gatatgtcat ccagaatctg gtggaaaatt
     9541 tatatcgttc ggcagaaaca ggagaagtta ttaccttatg aaagtagaag aaaaacagtt
     9601 tgatttagag tatatcaata agtatgcgag tcagatgacc attggaaatg gattttttgg
     9661 tatcagagga agccaggagg aagactatcg gaatcaagta aggggaatgt ttgcagctgg
     9721 tgtatataac cgacctttag gagcagaatc agccgagtta gtgaatctcc cagatgtcat
     9781 cagatgggaa ataaaaatcg acggtgaaca tttttctctt gagacgatga aagttcttgc
     9841 atacgagcgg gcactagatt tagaaatggg agagcttaaa cgctatgcgc tcattgaaac
     9901 tgctcaagga aaaaaagtgg aagtcattac ttgtcgtgtt gctgatcaaa ataatctcca
     9961 tcaggcagcc ctgaaagtgg tcatcaagcc gttgaccgaa gactgccaag tagatgtgaa
    10021 aacgggtatc gatggacagc aaaccaattt tggtacacag cagttggttg aaaaagaact
    10081 gcgagtattt ggtgacgatt tgatcagtgc atcctaccag acaacagaaa gtaaaattga
    10141 tattgggatt gcagctaaat tcaataaagc aggtgtattc caagcaaaaa atcgccggat
    10201 caccgcttcc ttttcagaaa agatcagaaa aaatacgtcc tttacgctgg agaagatcat
    10261 cgtgctctct acctctcttc agcaaacaga cccgtttaag aaaagtattg cccaagccca
    10321 gaaaagcgtt agttatacag aaattttaga gcagtcgatg tcttattgga cagatttttg
    10381 gcatcagcat cggataacga tccaaggcga ttcttctttc gatcagttag cgatggactt
    10441 cgctgcttac catttacaga ttatgactcc ccgcaatgat ccaagtgtta gtgtaggggc
    10501 gaagggacta actggcgaag gttataaagg gcatgttttc tgggatacgg aaatttttat
    10561 cctgcctttc tttttatatc agtttcctga agtagcaaag aatttgctgc tgtaccgtta
    10621 tgatcgatta gaaggcgcac ggcaaaaagc aagagattat cactatcaag gtgcactttt
    10681 cccatgggaa agcgcacaca ctggtcagga agagacaccc aaatttgctg cattgaatat
    10741 caaaaccgga acaagacaag aagttgcttc agctgccgca gaacatcata tcagtgcaga
    10801 ccttgcttat gccgttcaag agtatgtcca agcaacagag gatcaatcgt ttttagaaac
    10861 aaagggaaaa acgctcattc atgaaacagc agctttttgg ctgagcagag ctgtaaaagt
    10921 cgatgatcat ttagagatac acagagtcat tggtccagat gaatacacag aatatatcga
    10981 caataatgca tatacgaact atctggcgca ttataatgtg gccttagctg ttaaatatca
    11041 agctggcgat ccgctttttt tgaaagaatg tcaaaatttt ttagataagt tatatttgcc
    11101 agtgcctaac aaagaacagc tgattccgca agatgacacg tatctcagta agccagttat
    11161 tgatttgacc acttacaaag aacgtcctgg cagccagtct attttacttg attatgcccg
    11221 tagcgaagta aatgaactcc aagtcagcaa gcaagcagat cttgtcatgc tgttatactt
    11281 attacctgag tatttttctc aaccaatcgt ttctaagaat ttgcattact atgaaaacaa
    11341 aacaatccac gactcatcgt tgagtaaagc gattcacgcc atagaggctt gccgtatcca
    11401 agaaatggaa tgggcgtatc atttattcca agaagcgtgc ctgattgatt taggaccaga
    11461 tccgcattca tcagatgacg gaattcatgc tgcttcatta ggagcactat ggctagcagt
    11521 tatatttggt ttttcaggta ttacagtaac gaatggaact ctttctattt ctccaagact
    11581 tccttcagca tgggaaaaaa tttcttttcc ttttatttgg aaaaatcaca ggttgctatt
    11641 tgttttgaca aaaaaacaaa tagtaataaa aaaagagacg aaagaaacat tacctatttt
    11701 tgtcaatgga gaccaatacc agctggaaaa agaactgatc ttggaacgga ggggaaaata
    11761 atgcgtattc tgattgcgcc agattctttc aaagaaagtc tgacagcctt agaggcagca
    11821 caagccattc aatcaggttt tcaagaagtt tttgttcagg ctgaatatga tctgctacct
    11881 ataggcgatg gaggagaagg aacggtttca tcattagcag acagtttaag gttgaagcgg
    11941 caaacagcag tcgttactaa tgcatttggc aaaaacaaag aaatccagta cgcttcagac
    12001 ggtcaaactg cgatttttga aatggcagaa atcgtcggat tgaaagatgt cccactaaac
    12061 caacaacagc cacttaggtt atccacgcga ggagtaggag aactgatacg acaattgttg
    12121 caacaaggtg ttcgacacat catcatcggc gttggcggaa gtagtaccaa cgatggcggc
    12181 atcggcatgg cagaaggtct aggttatgct ttttacgatc aagataatca aaaagtagaa
    12241 gcactgggag aaaatctagg taaaattgct acagttgata aaacgaacgt gcctattgag
    12301 ctgattaaag ccaaaatcac tgtcattact gatgttacta atcttttatg cggaaaagaa
    12361 ggcgcttctt ttgttttcgg accacaaaaa ggcttgaaag aaaatgaatt agcagtggct
    12421 gaccaagcca tgcatgattt ttatatgaga tttgcccctc aagtaatcga ggcatcgggc
    12481 agtagtgctg gtggtggaat gggtgcagga ctgttagcat ttgcttcagg gaaaatagtc
    12541 tctggcatcg actacgtatt ggatttactg gagttcgata cgggtacagc aagcagacct
    12601 cgtaatcgta ggcgaaggac gtctagatgg acaaagtcta aaaggaaaag cgccagttgg
    12661 gattgctaga agagtaccag agggcgttcc tgtgatagct atttgcggaa gtgttggtaa
    12721 aggaagcgaa aaagcagcag atgttggcat ttcggccgtt tttccgatcc ttcctgcagc
    12781 tgtgccttta caagaagctt tacagcaagc cactgataat cttcatcgca cagctaaaaa
    12841 catcgcatct ttacttcaag tcgttcattt tcaaggaggt aatcgatgaa aatcaaagca
    12901 gcactattcg atttagatgg cgttcttgta gacacagctc gttatcatta cgaagcgtgg
    12961 ctggttttag ctaaccagct gtccattcca ttcactgaga aagaaaacga gcaactgaaa
    13021 ggaatatcca gaaccgagtc actagagcga ttgctgtcat ttggaaaaat ggaacagaaa
    13081 ttctcagaaa aagagaaatc agcctttgct gagcaaaaaa acaatctata tcttcaagcg
    13141 attcaaaaaa tggatgaaac atccgttttg cctggtgcaa tagctgttct tgagtacttg
    13201 aaaaaaacaa acatcaagat cggattggga tcagcaagca aaaatgcacg cctcatttta
    13261 gaaaaaacga atttgacaag ttattttgat gtccttattg acggcacgca agtatcaaaa
    13321 gcaaaacctg atccagaagt ttttctcaaa ggagcacagc aattgaatgt accgccaaat
    13381 gcatgcttgg tcattgaaga ttcagaagct ggttgtcaag cagctttggc tggtaacatg
    13441 catgtattgg gaataggtga aaacataaat cttccttctg ctgaatatgt gattcctgat
    13501 ttaaccgttt ttgatcaagt acgttcgttt tggcatttat cagaagccgt atcacgtata
    13561 taatagtttc cgttttttct gtcacaagca aaacgttatc ctccttttgg aaatcaaggt
    13621 atgatgagaa aaaaggggga ttttatatga caaaaaaagc agttttcgca ggcggatgct
    13681 tttggtgtat ggtcaaacca tttgaagagc agccagggat catttctgtg acatccggtt
    13741 atactggcgg gcatgtacca aatccaactt atgaacaagt aacaacaggc acaacagggc
    13801 atacagaagc agtagagatc gaatatgatc cagaagttat ttcttatgat cagttggtgg
    13861 aaatctattg gcagcaaacc gatccgactg acgcattagg acagttcgct gatcgcggtg
    13921 attcttatcg tccagtgatc tactataatg atgaagaaga acgaaatatt gcagagcgtt
    13981 caaaacaagc attgcaagac agcgggcgat tcgataagcc aatcgtgaca aaaatcgaac
    14041 caagaagcga attttatcct gcagaagatt accatcagga ttattacaag aaaaacaagc
    14101 tccattacaa tctttatcga gaaggttctg gacgtgcagg tttccttcgt cgacattggg
    14161 gcgtcaagaa ataagaagcc gaaaaagcta cttatggaac accgtatatc agaggttgta
    14221 aaaccagcat tttgagctaa tactgagggt cagctgattg aacaccattt agtggagagg
    14281 ttgtgaacct gtcgcctagc tccaagtcac aaggatttgg taagagaaca ctatatatca
    14341 gaggttgtga caagtcgttt gtcacaacct tttttcaata cctaagatga aggacaatca
    14401 gcaacgcact gctcaaaaaa ggaatgaaag gcagcttttc caactttttt ttatgacaga
    14461 taaagtaaac agaaaaaacg gatattccaa acagactagc aagaaacaag atctgaagca
    14521 actcttctag agacaagcca ccactccaag aaagcatcaa cagagtatcg ccagctccga
    14581 gataggattg accatagcga aggcacacac cgcctacgat aaacgttaac agcacacttt
    14641 gccattggaa gacgccggcc tgcaaccaaa aaatccataa aattgcccaa gaacagaata
    14701 gcgtgtgaga atggaccatg aagtaaaata aatccatcaa cgaaaatagc aaagccgaaa
    14761 gcatccatat gaacgtcgaa aaatttcttg cggaagaaaa agtaaagaac caggcaaaaa
    14821 gaccaccaca taaggcttct gccaacaaat agtagaaggg caaccgacac ctgcaatagc
    14881 ggcagcgaaa tcgttggacg atgatcgaga gaatcggaat caattcatac caagaaagcg
    14941 aacgacagca tttgacacaa tgagatctag gataaataat agagtgacct aacggaatcc
    15001 gctgggcggt taaacataaa aatgatccga tacagcaacc aatgataaaa taaagtaaca
    15061 cgccatctcc cccttttcaa aaccaagata cgcaagctta aactaaattt ttgtacatac
    15121 ataaaatttg aggtgaaaaa atgaacgttt caatcggatt agatatcgga accacacaaa
    15181 ccaaagcagt tgctttcaca gatgaagccc aagaagcagc atccgcttat tttcgctacc
    15241 ctttgatcca agaaacacaa gggatggcag aacaagatca ggaattgatc tttcaaggtg
    15301 tctgcaccgt tataaaagaa gtaattaaac agttgccttc agacacaatc attcaatttg
    15361 tctccttctc ctctgcgatg catggcctga tattgttgga tgaagagaaa acaccgttat
    15421 ctcgaatgat cacttgggca gataaccgag cagcggacga agcagaaaaa ctgaaatata
    15481 ccgtgcttgg caaagaattt tatcggcaga ctgggacgcc gattcatcca atgacaccat
    15541 tgaacaaaat caagtggctg caaaacagcc aacctgaatt attcaaaaaa actgcttatt
    15601 ttgtcggtat caaagactat atcttttata gattgtttgg cgaactgatc agcgactacg
    15661 gaacagcttc aggaactgga tattttgata tccatgaatt ccaatgggca gaagaagtgc
    15721 ttgactactt gaacattcga atagagcaat tgccgcaagt ctttccttcc acctatcaag
    15781 taacaggtct accatcaaaa acagcagcag aactaggttt gccaaatgat atatcatttg
    15841 ttttgggagg ggcagacggg ccgctttcta atctagggtt aggtgctttt ggtgatacga
    15901 tcgccgcttt gaccgtagga acgagcggag cacttcgttt tattactact cagcctcagt
    15961 tccatccaga aatggagacg ttttgttatg ttctggatca gaaacattgg gtagtgggag
    16021 gagcgactag caatggtgct ggcatctttg attgggcagg gcatacattt ttacaggaag
    16081 tcgttcaatc tgctaaagaa aaaggagaaa atccatatga tgcactgctt tcggctatcg
    16141 cttttgtgcc agcgggtgcc aatggtctga tttttcatcc ctatttactt ggtgagcgag
    16201 caccgctttg ggacgcagat gcgtttggca gttttatcgg tttacagcgg caacatacag
    16261 aaaaagaaat gatgaaggcg attctagaag ggatctgtct caacctttat cggattttaa
    16321 aaggagtttg ccaggaaaat cagctcacag agatccgtgc tacaggcggg tttgctcaat
    16381 ctccgatttt tcgtcagatg atggctgatg tactgggtta cccgcttgtt ttccctactg
    16441 tcacagaagc atccgcatta ggagctgtga ttttaggctg gagaagcatc ggaaagatcc
    16501 aaagtatcag tgaagcacag gaattgatag aattgaaaga gcaagttgga gtacaagaaa
    16561 ctgatcataa aaaatatcag aaaatctatc cattatttgt ttcgacacaa aagcatttag
    16621 cacagactta tcagcaattt gctcaactgc gagaagaatt agctgattct gaaaaatgat
    16681 tggctttacg gataaaaaag cgaactgaaa atcgttctca ggtaaatgat tttgttgtac
    16741 ttttcattga aagtctacta ttattttaag tgtaagaaaa tgttaggagg caagaaagat
    16801 gaaattccaa caaaccaaag aagtattaaa tcaattagtt gcagatctaa gccaaatgtc
    16861 tgttgttgtt caccaaacac attggtacat gagaggacca ggtttcctta cattgcaccc
    16921 aatgatggat gagttcatgg atgatttgaa cgctcagtta gatgaagtat ccgaacgctt
    16981 gatcacatta gacggttcac cttattctac attgcgtgaa tttgcagata acacaaaaat
    17041 ccctgatgaa attggccgct gggatcgtac tattccagaa cgtttggaaa cacttgttgc
    17101 aggctatcgt tatctagcag atctttacca aaaaggaatc gaagtttctg gagaagaagg
    17161 cgacgatcca acacaagata tctttatcga attcaaaaca gccacagaaa aacgtatctg
    17221 gatgatccaa gctcatttgg gcaaagcgcc agagatcgac gcgtaaaaac tgttgttgaa
    17281 cttgaccgaa aaagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg
    17341 tcaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga
    17401 acaccgttta ccaagaggtt gtaagcctgc cgttcagctc cgagtcacaa gaaacaattt
    17461 taaaaaacag cttcttatgt tttatggata ttgtgacttg tgaccgagga gttggcagga
    17521 gaacaccgtt tattcaaaat aaggggttgt gacaagactt ttgtcacagc ctctttttgt
    17581 ttttcatttt gacaggaaac gaccctttta tataaaagaa agaaaagaag taaagtttca
    17641 tataaatttt tcaatcaagc agaaaaacgt tgacattgac tagattagcg ggtaatatgt
    17701 taaatggtat tgtttgcccc acaaagagcc ccggaaactc gaagtgtatg tcaaacatca
    17761 gaacttaaat tggaggaaaa taacgtgcgt acaacatata tggccaaacc aggcgaagta
    17821 gaacgtaaat ggtatgtagt agacgcaact gatgttccat tgggacgtct ttcaacagtt
    17881 gttgcatctg tactacgtgg taaaaataaa ccaactttca cacctcattt ggatactggc
    17941 gatttcgtca tcgtcatcaa tgcagacaaa gtgaaattag caggtaaaaa agcgactgac
    18001 aaaatttatt accgtcacag caactaccca ggaggattga aatcaatcac tgctggcgaa
    18061 ttgcgtgcta aaaattctcg tcgtttgatc gaaacttctg taaaaggtat gcttcctaaa
    18121 aacactttag gccgcaaaca atttacaaaa ttgaacgtat acggtggagc agaacatcca
    18181 catgcagcac aacaaccaga agttttagac atcacaaact taatctaata ggagggaatt
    18241 tcattggcac aagttcaata tatcggcaca ggccgtcgta aaaacgcagt tgctcgcgta
    18301 cgtctagtac caggaactgg caaaattact gtaaataaaa aagatgttga agaatacatt
    18361 ccacatgctg acttgcgtga agtaatcaat caacctttcg ctgttactga aacaaaaggc
    18421 gcatatgacg tatttgttaa cgtaaacggc ggaggatacg ctggacaatc aggagctatc
    18481 cgtcatggta tcgctcgtgc attgctagaa gttgatcctg acttccgttt agctcttaaa
    18541 cgtgctggct tacttactcg tgacgcacgt atggtcgaac gtaaaaaacc aggtcttaaa
    18601 aaagctcgta aagcttcaca attctcaaaa cgttaatatt ctttttatat caatatttca
    18661 ggacactttt caattatttg gaaagtgtct ttttttataa taagaacaca tttgagaaca
    18721 catgttaatc acacttattc aatccttttt gaaatgcttc aaaaacagat ttatcaacta
    18781 cgtcaggtgt gttcacgtat atttttgtag tagaaatatc cgagtgcgtt aaagcattag
    18841 agatttgatt catatttgct ccaccttccc tagctatagt tgcataggta tgacgaagtt
    18901 tgtgtggaga taattgagcc aattcaggat gccttttttt tattgcattg attcgatagt
    18961 ttaagtagtc agcgtgaaca ggacaattta cttctccaga aggtttggaa taggtaaaga
    19021 ggtactggtc aggtgtttgt ataattttta gtttcaacaa ttgttcagcc tgagctgatt
    19081 tccattctct taataatgtc atcacaactt ctggtgcctg aataacggtg tctttccaac
    19141 cttttgtatg tgtctttctt tgatatttat ctaaagcata tttgagacga accgtatttt
    19201 tattaaaatc aatatatttc cattgcagag cataagtctc actttttcta tcacctagat
    19261 ataaggtaag catgaaaagt gtgtaatcgt gaagtgtaag caagtgatta tttaagtcat
    19321 tgtttactgc ttctatccaa tcagtcagct cttctgatga taatgcttga ttcccttccc
    19381 ttatcctttt ttcttctaaa gccattttct taggcgcggt aatacattga ataattttgg
    19441 tagttcggtt gaattcgata taacatagta tttcggcaat atcaaacata gagcgaacat
    19501 agcctttgat aatttttata tttgcgtatt ctttcgattt ctttgttaat tcatctgata
    19561 ttatctcagt atttgaattc agataattca ttgcatattc accaaacatt ggaaggatgt
    19621 gcaatcggaa tatatcctta gtattactaa ttgttacatt acttggagcc ctgtttgacc
    19681 tagtagtttg acctagttca tatctaggaa gccattttga ttcataaaaa tctttaaatt
    19741 ttatttttcc tttcaattct agtgagtttg cattctcctc tcttaaaact gtttcaatct
    19801 tctttttcat ttccctttcg gccttaatag ctaattgcct agttggaaat actttttgat
    19861 acttctctga tgcaattcca gtgatttctc tgaatttttt tggatacctc agattagttt
    19921 tatatttccc atttgaaagc tttgtaggca aacttcctca ctcctttaaa aagtaaggca
    19981 gtacatttcg ctatttatag tatatgtgac gagaagaaaa tttcaa
//
LOCUS       DAYYKN010000048        19577 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000048 DAYYKN010000000
VERSION     DAYYKN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.048"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..1293
                     /locus_tag="ACHBB7_001405"
     CDS             52..1293
                     /locus_tag="ACHBB7_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM9209364.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            1402..2451
                     /gene="recA"
                     /locus_tag="ACHBB7_001406"
     CDS             1402..2451
                     /gene="recA"
                     /locus_tag="ACHBB7_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM9209365.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            2862..4418
                     /gene="rny"
                     /locus_tag="ACHBB7_001407"
     CDS             2862..4418
                     /gene="rny"
                     /locus_tag="ACHBB7_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM9209366.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(4497..5303)
                     /gene="proC"
                     /locus_tag="ACHBB7_001408"
     CDS             complement(4497..5303)
                     /gene="proC"
                     /locus_tag="ACHBB7_001408"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM9209367.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            5444..8017
                     /gene="mprF"
                     /locus_tag="ACHBB7_001409"
     CDS             5444..8017
                     /gene="mprF"
                     /locus_tag="ACHBB7_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM9209368.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            8171..9022
                     /gene="ispE"
                     /locus_tag="ACHBB7_001410"
     CDS             8171..9022
                     /gene="ispE"
                     /locus_tag="ACHBB7_001410"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM9209369.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            9267..10205
                     /locus_tag="ACHBB7_001411"
     CDS             9267..10205
                     /locus_tag="ACHBB7_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM9209370.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            10228..10908
                     /locus_tag="ACHBB7_001412"
     CDS             10228..10908
                     /locus_tag="ACHBB7_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9209371.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            10881..11705
                     /locus_tag="ACHBB7_001413"
     CDS             10881..11705
                     /locus_tag="ACHBB7_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM9209372.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            11820..12899
                     /locus_tag="ACHBB7_001414"
     CDS             11820..12899
                     /locus_tag="ACHBB7_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9209373.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(13289..13867)
                     /gene="pgsA"
                     /locus_tag="ACHBB7_001415"
     CDS             complement(13289..13867)
                     /gene="pgsA"
                     /locus_tag="ACHBB7_001415"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM9209374.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(14076..14999)
                     /locus_tag="ACHBB7_001416"
     CDS             complement(14076..14999)
                     /locus_tag="ACHBB7_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM9209375.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(15737..17023)
                     /locus_tag="ACHBB7_001417"
     CDS             complement(15737..17023)
                     /locus_tag="ACHBB7_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9209376.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(17016..18284)
                     /locus_tag="ACHBB7_001418"
     CDS             complement(17016..18284)
                     /locus_tag="ACHBB7_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9209377.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            18536..18997
                     /gene="mscL"
                     /locus_tag="ACHBB7_001419"
     CDS             18536..18997
                     /gene="mscL"
                     /locus_tag="ACHBB7_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM9209378.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(19056..19502)
                     /locus_tag="ACHBB7_001420"
     CDS             complement(19056..19502)
                     /locus_tag="ACHBB7_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9209379.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         5919 a         3765 c         4132 g         5761 t
ORIGIN      
        1 aataaaaaaa tataaaaata cagcgtatac tatataaggg agcgggaagt aatgaaagca
       61 gaaataatag ctgtcggaac agaactttta ctaggacaag tcgtgaatac gaatgccact
      121 ttcttgtcag aacagctagc tgatctaggt atcgaagttt attatcaaac agttgtagga
      181 gataatcagc agcgtttaga agagttgatt gctctagctg aaacaagaag tgaactcata
      241 ctcttatgtg gcggattagg accgacagaa gatgacctga caaaagaagc aactgcggct
      301 cacctcggta agtctctcat acaaaatacg gaaggataca aaaaattatt ggcatatttt
      361 gaaacaaccc atcgaaaaat gaccaaaaac aatctgcaac agtcccagat tatcgaaggc
      421 ggggttcctc taccaaatcg tacagggctt gctttaggga ctttttatca aacagataca
      481 catgcgtata tccttcttcc cggtccgcct aatgagttga aaccaatgtt tgtggaacaa
      541 gtacgtcctt tactagagga acgttttcct tctgaagaaa aactaatttc taaagttttg
      601 cgcttttatg ggattggtga atcgcgactg gtcactgagc taaaagatct gattgagaca
      661 cagatcaatc caacgatcgc accatatgcc aaaccaaatg aagtgacatt acgactgaca
      721 gtgaaaacaa atgatgttca ggcagggaat caagctcttt tagcattaga agaaaaaatc
      781 caagaacgag taggggaata tttttatgga tacggcgatg ataattcgct agcaaaagtc
      841 gtagtggaac tactgaaaga aaacaagcag actgtaacag cagcggaaag tttgacagcc
      901 ggcgccttcc aagccgcatt aggcgacatt gctggtgtat ctgaagtatt tccaggcgga
      961 ttcgttacct actcgctcca aacaaaagct ggttttctgg agattgatcc ggagctatta
     1021 gcagaatatg gaacagtcag caaagaatgt gtggagcaga tggccattca agcacgtgag
     1081 atagcaagaa cagattacag tgtagctttc accggagtgg ctggaccgga cacgttagaa
     1141 ggtcaatctg caggaacagt atggatcgct atttcatcta aagacggcgt ggtttcggaa
     1201 aaatatcact ttgccagaga tcgtgctgct atcagatata atgcggttat gcaaggattt
     1261 gatcttttga gaaaacagct tttaagaaaa tgataaatgc gaatatttgt tcggtttttt
     1321 cttgcttttt ttcactaaaa aagatatgat aaggataatg aaatgttgct ctgatttgta
     1381 aatataagga ggattatcgt attggcagat gatcgtaaag cagccttaga tgctgcactg
     1441 aaaaaaattg aaaagagcta tggtaaaggc tctattatga aattgggcga aaaaatcgac
     1501 caacaaatct ctacaattcc aagtggctct cttgcgttag atgtcgcttt aggtgtcggc
     1561 ggttaccccc gtggacgtat catcgaagta tatggacctg aaagttcagg taaaacaaca
     1621 gttgcactac acgctattgc agaagtacaa aaaaatggcg gaacggccgc tttcattgat
     1681 gctgagcatg cgttagatcc gcaatatgca caaaaattag gtgtgaatat cgatgaacta
     1741 cttctttcac agcctgacac aggagaacaa ggtctagaga tcgctgatgc tttagtatca
     1801 agtggggctg tagatatcgt agtagtcgat tcagttgctg ctttagttcc acgagcagaa
     1861 atcgacggcg aaatgggtga ctcacatgtc gggttacaag cacgtttgat gtctcaagca
     1921 ttgcgtaaac tctctggttc gatcaacaaa acaaaaacaa tcgctatttt catcaaccaa
     1981 atccgtgaaa aagtaggcgt gatgtttggg aatccagaaa tcactcctgg aggacgcgca
     2041 ttgaaattct acgcaacgat ccgtttggaa gtacgtcgtg cagaacaatt gaaacaaggt
     2101 acagatattg ttggtaaccg tacaaagatc aaagtcgtaa aaaacaaagt agcacctcct
     2161 ttcaaaatcg ctgaagtaga tgtcatgtat ggtttaggga tctcacaaga aggagaactt
     2221 ctagacatgg cagtagaaaa agacattgtt gataaaagcg gtgcttggta ttcttataaa
     2281 gaagatcgga ttggtcaagg acgggaaaac gctaaaatct atatggctaa tcatcctgaa
     2341 atgatggcag aagtttctgc tctagttaga gcagcttatg gtattggtga agaagtcgct
     2401 gtcccagaag atgaaaaagg acaagaagaa ttgcctttag tagaagaata aaaattatga
     2461 taaacaaaaa cggctggtct gaagagtagg tactctcctt gacctgccgt ttttttattg
     2521 aataaagaaa tcgtgagtta tttaattata cgagtgggta cataaaataa aaatatctgt
     2581 tcatctacaa agaataattt aatagaaaag tttctcaatt taaaggttta cctatatgaa
     2641 actagtgtca tttattttaa aaaccatcgt ttaatgaata agtagaaagc cgtttctttg
     2701 tttatttaaa gttgacacta cggggtacaa acattagaat aaagttgtgc atgttttttg
     2761 tacacatata tgcgaagatg catattgacg agtttagaat aatttatcat agaagctgta
     2821 tagatgataa aaatgaaaaa gatagtatgg aggtggattt tatggtattc aacattctcc
     2881 tcgctatcat cggtttaatt gtcggtcttg gtttagggtt tatggtcgca aagtcacgtc
     2941 acgagaaaga aatagctggt gcacaatcat cagctgctgg aatcatcgat agtgcaaaaa
     3001 aagaagcaga aactctaaaa aaagaagcat tgttagaagc taaggaagaa aatcagaaat
     3061 accgttctga agttgaaagt gagctgagag aaagtaagtt agaattaaaa tctcaagaga
     3121 atcgtttgat tcaacgcgaa caaacgctga atcgtaaaga tgactcgtta gaaaaacgcg
     3181 agaactcact tgaaagtaaa gaagaagcac ttagttcaaa acagcaatta attgaagagc
     3241 gagaaaaaga cgttgaaaaa atgattgaag atcaacaaaa agaactagaa aaaattgctg
     3301 gtttatcaaa agaggacgca cgagatgtca tcatgaaatc gactgaagaa gagctgaatc
     3361 atgagttaac tctaatggta aaagaatctg aacagcgtgc caaagaagaa gcagatcgca
     3421 aagcgaaaaa cttattatca ttagcgattc aacgctgcgc agcagatcaa gtttcagaaa
     3481 cgactgtttc agtcgttacc cttccgaatg atgaaatgaa aggtcgtatc attggacgag
     3541 aaggtcgtaa tattcgaacg cttgagacgc tgactggtat cgatttaatc atcgatgata
     3601 caccggaagc agttgttctt tccggattcg atccaatccg aagagaaatc gcacggatga
     3661 ccttggaaaa actgatacaa gatggacgaa tccatccagc gcgtatcgaa gaaatggtcg
     3721 agaaatctcg taaagaaatg gatgaacgta ttcgcgaata cggggaagag gcagcgtttg
     3781 aagtcggtgc tcatacactt catccagatc taatcaagat catcggacgt ctgcgattca
     3841 gaactagtta tggacaaaac gtcttgaagc attcggtcga ggttgcaaaa ttagctggta
     3901 tcttagcagc agagcttggc gaagatatcc aattagcaaa acgtgccggg ttgctccatg
     3961 atattgggaa agcacttgac catgagattg aaggatctca cgttgagatc ggtgcggaat
     4021 tagctgccaa gtacaaagaa aacccagtag taatcaacgc gatcgcttct catcatggag
     4081 atgtggaagc tacttctgtt atctctgtcc ttgtagctgc agcagatgcg ctctcagcag
     4141 caagacctgg tgcgcgaagt gaatcattag aaaactacat ccgtcgttta agaagtctgg
     4201 aaaatatttc gaacagcttc gccggtgtag aatcaagtta cgcagttcaa gctggacgcg
     4261 aagttcgggt aatggtcaag ccagaagaaa tctcggattt agacgctgtt cgtttagtac
     4321 gtgatattcg taaacgaatc gaagaagaac tggattatcc tggacacatc aaagtaatcg
     4381 ttattcggga aactcgtgca actgattatg ctaaataata aaaaatacaa aagaagagcc
     4441 gcggcaaaag ccacagctct tctttttttg taaggcacga aaaaacattg ctatcattat
     4501 tttttattca acatctcaag atctttctcg atcgtcgtat tgattccttt gatgattgct
     4561 gggatgaatc ctgcttcttc taaggcaact acacctgcaa cagttgtacc gccaggagaa
     4621 gaaacttggt ctacaagttc ccatggattt ttatcacttg aaaggaattt ttgacaagaa
     4681 cccataattg cttttgcagc aatctcagtg gctgtctgtt tggacaaacc attcagtaca
     4741 ccagtccgtc ccatcgcatc gatcagcatg taaacgaatg cgggtgagct gccagcaagg
     4801 ccaatgaatg tactgaaatc tgattctgct aaaggataga cgctgccgat tgcttcaaaa
     4861 atcatcgtag cttgctgaag ctgttcctcc gtggcagctt catttttgca aatcgcagaa
     4921 acgcctgctc ctacgctgac attgacattc ggcatcacac gtacgatgga ggcctgagga
     4981 tcttcaagcg ctgtttctaa atctgttaat gattttcctg aagcaataga aatgaataag
     5041 actttctggc tatattctct tagttcttcc aatacttttt caatgatttt tggtttcacg
     5101 gctaaaagaa cgatatctga ttgtgatgca acttcctgat tacttttgca tgcatggacg
     5161 ccagtttcag cagcaaattt ttgtgcattt tcaaaatgtc cactggaaac gaacacctct
     5221 tctttcgaga ctgttccttt ttttaataaa ccatcaataa tagcttgtgc catattccct
     5281 gtgccgataa atcctacttt catgttatcc tctcctttag cattaggata tctttctttt
     5341 tctatcatcg caagcaaaga acaggtctaa agtggaaaaa ttaaaataaa taagataaca
     5401 ttggggtatg acacaaaagt atacaagtta ggaatgggaa aatgtgaaaa ataaacgata
     5461 cttgatgctg caatggataa aggaacatag tctgatttta aaactgattt ttttcggctc
     5521 cgtgttagtc tttgtggcaa atcaagtcgc aaatattgct aatggcatga gttggcaaga
     5581 tatttggcaa aggatgggac aacaaagccg catgacgatc ctgctcatga ttctggctgg
     5641 actgctgggt gtcactccga tgttgttgta tgattgggtg acgatcagag tcttagaaaa
     5701 acaaggaaaa ccaaaaatgg aaagaaaaga gtttcttctt gctgcgtggg taacaaacac
     5761 gatcaacaat ctcgccggtt ttggtggtgt ggtaggagca agtctgcggg ctagttttta
     5821 tggaaagcag acaaatagaa aaatggtttt agccactgta tcaaaagtcg cactatttat
     5881 gctgactggt ttgtctgtat ggtcgtttct tacatttatt gatgtctttt ttattcagtc
     5941 agaaagtatt tttcgaagct attggatctg gcttttcggc ggtagttttt tagcaccagt
     6001 cattttcttt tttgcttatc taaagagaaa gacattgttc ttcgagtttt atccaaaagg
     6061 cttgcttggt ctgctactgg catcctttgg acaatggacg ggagcccttg cggtattttt
     6121 gatcatcggc aagttgatga gaatagatat ttcgatgctt tcgatctatc ctatgttcat
     6181 gattgctaca ttgataggaa tgctgacgat ggtcccaggt ggaatgggga cttttgacgt
     6241 tttgatgatc ttaggaatga gtcaattgcc tgttcggcaa gatgtagcag tagtttggct
     6301 attatattat cggttgtttt attatgttct accttttatg acgggacttc tattgttcat
     6361 cacacataca ggagtcaaaa tcaatcgatt tttagataac attccgcgac tgtttttcca
     6421 aaaggttgcg catatcgtgc tcgtaagtgc tgtctatttt gcggggatca tgatggtact
     6481 tctatcaaca atcactaatc tgtctgcaat cagtcggcta tttcaagtat tattgccgtt
     6541 ctcattcaat tttttagatc agacagtgaa tctattggtt ggctttttat tactcggcct
     6601 tgctagagca ctttcagtaa aagtcaaaaa ggcatttctg cctacgattg ctttactatc
     6661 ttttggtatt ttaaatacaa tcacacgtac gttatcttgg cagttgatat tggtatatgt
     6721 cttgattctg ttagctgtgt ggttatccag aaaagaattt tatcgaacaa aattcgttta
     6781 ttcttgggga gctataatct ttgatgctag cttgttcggc tttttgttta tcgtttacgc
     6841 aattgcaggc tatcacagcg gacgcttttt agtcggtcga ataacaggaa atcattttat
     6901 tttgtttcct tcagatgatg tgtggttctc agggcttatt ggtttgggca tctcattgct
     6961 agctttagtg gcgctttatc aatatttagc tgaagatact acgaagcttg gcagtgagta
     7021 cgaaaaagac cgttttgacc gtttattgga aacatacgga ggcacagcta caagccatca
     7081 gcttcgcttg tcaggttaca gttattttta ttaccaagaa gacgaccaag atcaagtcgt
     7141 ttttggttat cagatcaaag gaaacaaatg ctttgtttta ggagatccag ttggcaatcc
     7201 taaaatgatg cgggcagcaa cgaatcagtt tatgaaacaa gcagattgtt taggctatca
     7261 gcttgcgttt tataagatat cagaaagcta cgtagtgatg cttcatgaat tgggttttca
     7321 cttcacgaaa gtaggtgaag cagggattgt tgatctagca acagaagact tgtctcggct
     7381 gagtcagcaa cttgaatatc aacgtttgat gaaagaagga taccatttta cttggtacga
     7441 tcaactgccg gaagaattac atcaagctgt aaatgaagtt tcacaaaaat ggcttcaagg
     7501 tgcacgagaa aaatacttct ctgtgggacg atttgataaa tcatatgtat tatctagcgg
     7561 tgtcggtatt gtccgtaaag atcaaaaagt cgttggcttc atcaccgctc agccgatgac
     7621 gaagaagcag gcgggatatg atttgttgcg ggtcttacct aaagaatctg aggaattagc
     7681 tgactatctg ttagccaatc tgtttattgt atacgagaaa atgggctact cggaagccaa
     7741 tctaggctta gctccattag ctaatgttgg agaaacagat ttttctttct ttcaagaaaa
     7801 agtcatgcat atcgtatata attatggtaa ttttttctat tccttccagt ctgcttatga
     7861 aaagaaatta aaatatgtcg atcattggga aggacgttat tttgcttata ggaaaggaag
     7921 taatttcgga tttgctacca tgcagctact cagtctgata ggaaaagaaa aaggaaatgt
     7981 tccgagtttg gcagaagaag tgctcacaga attataagaa tcactagaac ttgtttggat
     8041 tatgaagaga aaattctgtg aaagctgttg aaacgatgac aaaatcaacc actaggcaga
     8101 cataagctat acttttattt ttccctatga tagaatactt gcgacttaga cttttcgaat
     8161 gggtgagagg atggaattga ttgaaaaagc gccagccaag ataaatttag gactggatgt
     8221 attatataga cggcaagatg ggtatcatga gttggaaatg gtgatgtcga gcgtagactt
     8281 ggcagatcat ctattttttg aagaaataac agaagacaaa atcattgtag aaacaaataa
     8341 agcattttta ccggtagatg aacggaacaa tgtttatcaa gctgctttat tgctaaaaaa
     8401 taaatacaat atcaaaaccg gtgtcaagat ctttgttaag aagaatattc cagtagcagc
     8461 tggattaggc ggaggaagta cagactgtgc agctgctcta agagggatca accaattatg
     8521 gaatctgggc tgttcgttag aagaactggc agaaatcggt ttggaagtcg gtacagatgt
     8581 tccttactgt gtctacggaa gtacggcatt tgtcggtggc aaaggggaag aaatccaagt
     8641 attaccttct atgccacagt gctgggttgt gttggtcaaa ccaagaatga gtgtctctac
     8701 acgtacagtg tttagagaag tagatacgca agccatccaa catcaagaca tccacggact
     8761 tgctgaagcc atccgagagc aagattatca gcagatgatc aaaaaaatgg gaaacagttt
     8821 agaagatata acgggaaagc ggcatccagt catccatcaa atcaaagagc gtatgatcaa
     8881 gtacggagca gatgctgcat tgatgagtgg aagcggacca accgtttttg ccttgtgtca
     8941 acattattca agagcgcaga gagtatataa tgggttaaaa ggtttttgtg aggaagttta
     9001 tttagtacga accctgcaat aaaatataag aagtaaaata ggtgatgaaa aactgaggaa
     9061 tcctaaaatg gtattcttca gttttttatt gtagtgaaat ggctagatat gagcagcaac
     9121 tcatccattt tacgatttaa tcaagaatat acatgcttaa atcagaaatt gatggatttc
     9181 tattgactgt gtttttttat tttgatacat tagttcacgt taataaatcg taaaattccg
     9241 atttgttcat tgaaaggagc atataaatga aaaaatatct tttgacagct acggtaatga
     9301 taggtgcttt actgtttgca gcatgtggaa atacgaacaa ggaagcagac aaaaaagagg
     9361 atttgacgat cgttacgact ttttatccga tgtatgattt tacaaaagag atcgtagggg
     9421 atgaaggaaa cgtgaaactt ttgatccctg ctggaacgga gccgcatgat ttcgaaccaa
     9481 gtgcaaaaga aagagcagaa atttccgatg cagatgtttt cgtgtataat agttcagaca
     9541 tggaattttt tgtggattcg ttaaaggatt cagtggatag caaacagacc ttgatgatcg
     9601 aagcagctaa aggaatcgac cgtttagaat cgcaagaagc agacgaacat gaagaatcag
     9661 aagaagggca tggtcattca catgaatacg atccacatgt ttggctcgat ccggtattag
     9721 ctataaaaga agtcagaacg attgctgaag aattaggcga aaaatatcca gataaaaagg
     9781 aaacttttac gaagaatgcc gatgcttata tcaaaaagct tgaagcactg gatcaaaaat
     9841 actccgatga gctaaaagat gcaacaaacc gcacatttgt cactcagcat gctgcttttg
     9901 cttatttagc gaatcaatac gacttaaaac aagtagcgat ttcgggcgtt tcaccagatc
     9961 aggaacccac accaagtcgc ttagctgagc taaaagaatt tgtgaagaaa aacaacatca
    10021 aagtgattta ttttgaagaa aatgcctctt cgaaggtagc tgagacattg tcgaatgaaa
    10081 caggagtgaa gctagaagtg ttgaatcctt tagaaagctt gactaatgaa caaatcaaag
    10141 caggcgaaaa ttatatttct gtcatggaaa aaaatctgaa agctttaaaa gaaagtatca
    10201 attaagtttt agaatggagg ttgtaaggtg cgttatatcg aaatatctga tttgaccttt
    10261 tactatgatg atgaacctgt actagaagat gtctcttatc atgtagatgc gggtgaattt
    10321 gtcattttaa ctggagaaaa tggagcagcc aagtcaacct tggtgaaagc atcgttagga
    10381 ttactgaaac cttcaaaagg aatgataaag attgctgaga aaaatcaaga aggaaaaaaa
    10441 ttaagtatcg gttatattcc tcaacaagtt gcatctttca atgcaggttt tcctagtacg
    10501 gtgatcgaat tagtccgttc aggaagatat ccaagaggcc gctggttcaa gcgtctctct
    10561 gaaaaagatc atcaacatgt acagcgtgct ttagaagctg tagggatgtg ggatatgcga
    10621 aatcggcgaa taggtgaatt gtctggcgga caaaagcagc gaatcagttt agcgcgtatt
    10681 tttgctactg accctgactt atttgttctt gatgaaccaa caacaggtat ggatgaagaa
    10741 tccagaaatg aattttatcg tcttctccga catagtgcgc atgtccatgg gaaatcagtt
    10801 ctaatgatca cccatgatca tgaagatatc aaacagtatg cggacagaca gatccgactg
    10861 gtgagaaagg aggattctca atggcgttgc tttcatatga attcatgaga agagcgtttc
    10921 ttgccgctat cttcattgcc ggaatcgccc cgatgctagg tgtgttttta gtgatcagaa
    10981 gacaatcgct gatggctgat acgctttctc atgtctcttt agcaggggta gcgttaggat
    11041 tctttttgaa ccttaatcct acacttacga ccatgctagt cgttgtatta gcagctgtga
    11101 tattggagta tctgcgaacg atctaccgaa cctattcaga aatctccatt gccattttaa
    11161 tggctggtgg tttggcgcta gcgttagtat tgatgaattt gagcggaggg aattcagcaa
    11221 caagtatcca atcctatctg tttggttcga tcgtgaccat tacatgggag caagtcatct
    11281 ttttaggaat cttattcgtt gccatcggta tattgttctt tttattcaaa cggccgatgt
    11341 atgttctgac ttttgatgaa gataccgccc acgtggatgg gctacccgtt catttgatgt
    11401 cgatgatgtt caatgtgatt actggtgtgg ctattgctgt gatgatcccg attgccggtg
    11461 cacttttgat atccgcaatc atggttcttc ccgcagcaat cggtatgcga ttaggcaaaa
    11521 gcttcaatgt cgtgattgct atcagtgttt gtgtaggatt tctagggatg ctgtcaggac
    11581 taactagctc gttttatcta gatacacctc caggagcaac aatcacatta gtctttattg
    11641 ctttgttcct gattgtaaat atcatcaaac gaatattggt tgccttcaga agaaaaaata
    11701 actaaattat aaaggcggat tttataatta acttagccat ttggaaataa aaaaacgcca
    11761 cgtgaattcc atgttatatt gaagttacca caactctcaa tagacaagga tgaattcaca
    11821 tgacgcaaaa ccatgataac acagtttcac gcaagggaaa acacctaacc tactcagaac
    11881 gttgccaaat cgccatcctg aaaaaggaaa actattccaa ccgtcaggtt gcggatgtcc
    11941 tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg cgggaccgtt actcagctta
    12001 accgccagaa tcagaacggg aaggtctacg actattatca tgacgtctac gacgccgatg
    12061 ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg cagacggccc ttatgggccg
    12121 acacggacgc cttcattgaa tgggcggacg acatgatgct gttggaaaaa tggtccccgg
    12181 acgtggtggt gggctttgcc agaaaacacg aattgttcga cccggccatt atccctggga
    12241 ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa aacgaagaat atggacctgc
    12301 tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg caaccgcccg aataaacggg
    12361 tgctagggaa atccattgaa gagcgcccgg aggcggttgg tacccggaaa accttcggcc
    12421 attgggaagt cgacacggtc gtaggaaaca agaccaagtc ggatgccgtg ctgctgacgt
    12481 tggtggaacg gcagacacgc ttcgaggtca ttctgaagct ggccgggaag gatgccgaat
    12541 cggtcaacca agcggtccga cagctgagag aacgggctgg tgcgtacttc ggccagctgt
    12601 tcaagacggt tacctcggac aacggctcgg agttcgcggg actgcacgat gccttgcagg
    12661 atgtggcaaa catctacttc agccacccct atgcttcctg ggagcgcggg acgagcgaaa
    12721 accaacacaa gctgattcgc cgtttcatcc ccaagggcag cccaatcggc aacgtgagcg
    12781 aaaaccaaat cttgcggatt cagcgttgga tgaacgatta cccgcgaaag attttgggct
    12841 atgcaacgcc gcatgacacg ttcgtccagg catttaggca ggaacggttg acggcttgac
    12901 acgtgggccc ttgacaacga gcctccctcg gcatggcttg tgtctggaaa gctggccggg
    12961 gccagccagt cgggagacta ccatgcctca gctcattgtc aagggcccac tcgattagaa
    13021 aaattagacg gttggtggta acgagcaacc gggtcaccat gggtggctaa cttaaacttg
    13081 aaatttacga tttttttatt taaaaagagg gcaaggagct gtgacaaaaa tcttgtcaca
    13141 gctccttgtt ccaaataaac agtgttctcc tgccaaaacc tcggtcataa gtcacaatat
    13201 acataaaaca tgaaaagctg ttttttgaaa ttatcccttg tgactcagag ttagacggca
    13261 ggctcacaac ctctccacta aacgatactt acattgatcc tttgaacaca ccgctgtttt
    13321 tgatgaagta atccacacct gaatagatag taaagatcag acaagcataa agcatgattt
    13381 gatctactgg aataccgatc aggctgaatg ggatattatt gataaacagc aagataatag
    13441 caaccatttg tgtagccgtc ttgacttttc ccggccaagc tgcagccatg acttctccat
    13501 gttcgacaag tagtaaacgc aagcctgtga cggctaattc acgacagaca atgatcgcta
    13561 ctacccaacc aggggccttt ccttggtcaa ccaacatgat aaaagcagtc ataactagca
    13621 ttttatctgc caacgggtcc gcaaacttgc caaaattagt gacaaggcct cgcgcacggg
    13681 caatttttcc atctaaccag tctgtgatac tagctaaagc aaaaataact gcacctacta
    13741 gctgtgtgac agccagtgtg gtgtctccta cgttaaattg tccccaatct aaaggtaaac
    13801 ctacaaccaa aatgaaaatc gggatcatac aaattctaag tacagttaat ttatttggta
    13861 aattcacact atcgactcct ttcttcccta ttctacgtga ctatcagcga agtttccacc
    13921 gatattattt acttttccaa atgaatttat tcaaaggagt atttttattg aaattgatct
    13981 ttttgaatta acgaagaagg accgcgacac agatttgtca cgatccttct tttttatacc
    14041 tacaagtaaa cagatgcgta aactaattta tacgatcatt ctgcgtattg aagagtaagg
    14101 ttcacatttt ttggactctg attgttttcc cctggattaa ctggtacgga ttctccattc
    14161 gctttgatcg ttgcattgct tgctgcacca atcgtgatgg tcgcatttgt tgctgtttcc
    14221 ggtaacacgg tactctgtgt ttcatttgct gccagtgtcc cttgataaac ataggcattg
    14281 tctactagca cacctaacca tacacgattg acagcagtaa attcaagggt tacaggcttg
    14341 gtagcgtctg tgatatttac tgcgatggca ctgccagaat cttctcctgt agctattgcc
    14401 attttctttt cttcacttga ttttgttgtt gattctactg tagaagactc tttggtctgc
    14461 gtgctttgct ctgaggaaga ggactcctcg actgatcctt ctactttaag cgaagaagct
    14521 gtgtctccga ttattggatc agcttgacga tcttgccaag tcatatagcc cacgacaacg
    14581 acaatcgcca aagcaaccaa tcctaataaa atgacaggaa gacttgtcca aaagcgactt
    14641 ggatgcgatt cttcctgatg cattgctttt ctagaagtac ttactgtttc aggctgcgga
    14701 cgttcaggga ccgatcctgt caatttgctt ttcccatcaa aaacatcgac taaatggtct
    14761 ccatcttctc ctacagcttc agcgtattgc cgaataaatg cccgcacgta aaatttacca
    14821 ggcaattggt caaaatcatc attgtcgatt gcttccaaat aacgtttttg aatttttgta
    14881 atctgctgta attcatctaa agatattttt ttattcaggc gtgcttgccg taatttttct
    14941 ccgatggttt caaaagccac tcgttcatct tctccagtct gtttgattct ccgaagcatc
    15001 tgctgttgca acgaaagacg ttatgtttgg gtgctgtgac aaaagtcatg tcacagctcc
    15061 ttttttccga ataaacattg ttctcctgcc aactcctcgg tcacaagtca caatatccaa
    15121 taaatataag aagctgtttt ttgaacttgt tccttgtgac tcggagttaa acggcaggct
    15181 cacgacctct ttcttaaacg gtgttcaatc agctgttcct cggtattagc tcaaaaaccg
    15241 gcaaaatata aggagatatt tttccgtatg tttctctaat actcaggtca aaacgctgat
    15301 ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc
    15361 aagttaaaaa cattaagaag ctgtttttac ttggctgttc ttattacttg atgcagggca
    15421 gctttttcac aacctcttgg tacacggtgt tccagaagta gcttcttcgg caataagccg
    15481 aaatatcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
    15541 ccacttctgt cacaacctct ttgattactt cgtttgattt tattttaata taagttgatt
    15601 aaaagagagc atgatttgtt ttattttctc actaattttt ccaacacaac taagaatacc
    15661 ataaaagctg attttggagg aggttcctac tacaagtcta aaacgaaatt taagactttt
    15721 cctcataaat actggtttat tttggtctca tgtaaaactc gctgaaggcc tcttcactta
    15781 taaatgcttc cccagctgcc agtacgtctt tcatttggat cgaatcgatg atttcaggaa
    15841 gatcaaataa agttcgatcg ccaaataaac tttgcgtaaa ttgattggca atatattcga
    15901 tcgaatttaa tgattggaaa tattgtccaa gcattttctt cttcagcaaa tcgagatttg
    15961 tttctgacac ttccggatca tcagcgaatc caagaatgat ttctttgact ttttctgctg
    16021 ctttctctgg ttcgtccgta tctccgctga agtccgcaaa gtgaaattca cgatccaagc
    16081 tgaattcaaa accaaagctg tcatcgataa ttccttgatt gtacatcgct agatagtttc
    16141 gagaagtatt gcctaaaatc atttggaaca acagattgat tgctgtttta tagcgtaaca
    16201 gttctgttcc ttcttgcggc aatgtgtcta atcctttaat accaagtaca aatttatctc
    16261 gtgtaatggc tgcttccaat gcactttgtt tgatgatttc ttttgtgttt tcagggaaat
    16321 aacgtacaat ttcttgcttt ggagggaaat tttttgcttc ttggttttcc cgaataagtt
    16381 tcatcaattt ctcaggctcc atcttaccaa ctacaaataa aaccatattg cttggctgat
    16441 aaaacgtccg atagcacgta tacaagtctt gagctgtgat tttgtcgata ctttcaaccg
    16501 tgcccgcaat atcgatatgc aatgggtgtg taggatacaa gttatttagt atgccaaaaa
    16561 acatccgcca gttagggtcg tcttcataca tttggatttc ctgaccgatg attccttttt
    16621 ccttgtttac agtttcttct gtgaagtaag gtgcttgtac aaaatcgatc agagtcgtca
    16681 gattcttttc tacctgatcc gtcgtagaaa acagatagct ggttttcgta aaacttgtga
    16741 aagcattggc ggaagctcct tgtttcccaa ataattgaaa gacatcacca tcttcttttt
    16801 caaacaactt atgttctaaa aaatgggcaa ttccatctgg aactttcacc ttttcttttt
    16861 caccataggg aataaattcg ttatcgatcg aaccataatt cgtactgaac aaaccatatg
    16921 tcttgtgata gtcattttta ggcaacaaat acacggtcaa accgtttggc agtacttcat
    16981 gataaagtgt ttcattgatt tgctcatatt ctgttttatt catttgcatt tcctcctgct
    17041 aaaaagaaga ttgcctgcaa ttcgatttgc tcagctactt gttgtatctc tttgatcgtt
    17101 acgctctcca tacgtttcaa ccattcttca tcagataatt ttgtttccgg cagccagctg
    17161 tccaaaaaag aagcttcgat tgccgcttgc ggagagtcta gagaaagcag atattggttt
    17221 ctcagcattg cttttgtctg agacagttcc aactctgtta tttcaccatt tcgcaaactt
    17281 tctaattgct cgtgaatcag atgaagtact tgatttcggt tcttttcatc gataccggtt
    17341 tggacactca tgaatcctcg gaacgtatcc acgctactag aggcataata agctagactt
    17401 tctttctctc tgacattcat gaaaagttta gagtgaggaa aaccgccaaa taaaccgtta
    17461 aagaccatta gcgcaaaacg ttctggttca tcgtaataca catttgtctg ataagctagg
    17521 ttcagctttg cttgaacgat tggctcacgt acttgtcgtt ccttcacgat attcacttga
    17581 ggttgggtat aaaacagatc tggataaact gtttcccgcg actcaaatgg taattgccca
    17641 actgcttcag ctgctttgtc cggatcaaca tcgccgacaa caaaaatgtc gatttgatca
    17701 tttgccatca tttcttgata gtaggacgct agcgaactag cagtgatctt gtccaaatct
    17761 tcgactacgc ccaaccctgg gatcttttgt tccgggctgt tttcaaaata aagttcttgc
    17821 aaggcatagg aagcaaaagt ttgtttatct tccttcaaac tttccaaata tagacgcaga
    17881 ttgttctttt ccaagtcaaa cgtcaactga tcaaactgat gatttttaat atttggataa
    17941 aataaaacct cttttaaaaa atctaccgct tgagaaaaaa ggtttggatc attgacatat
    18001 tcaccattca caaaactgat ccctgcgttt agccaatgta aatttccttt ttttctaact
    18061 gataatccaa agcttgcgcc ataaagctct gcaagttgcg cacttaactt tgtctgatcc
    18121 ggataattca aactatttgt ttccaacatg ctgctgagaa gtgaacgttt cgtgatgact
    18181 tcttgttgca gacgcgcagt aaaacggata aaaatccgga tcgttttgta ttgttttgtt
    18241 ggtaagacat gaagattcac gccttctgcc aattgataca ccatatttat atgctccttt
    18301 tcacagttga ttgcccgctt actcttttaa tgggtcaacc tgcctttttt acttattcct
    18361 ctgcttgaaa tttataagtt caggcaattc gccgtttttt ctaaatgatg ttacacttta
    18421 tagtatagca tagatgtttt ttatcaaaag aaacagacgg ctaaaagcgt atattgtttt
    18481 tttatttttt atttttcgat atactaaaga caaataaaag aagggagggt cattcatgat
    18541 caaagaattc aaagagttca tcatgcgtgg agatgtcttg gatcttgctg tcggcgttgt
    18601 tattggtagt gcgtttacaa gcatcgtaaa ccaagtggtg caaggcttga tcacaccact
    18661 aataggttgg tttgtcgcaa tgatcacagg tacttctgat ttagaaggag cactttcaat
    18721 tcttgactgg tcgccgacaa aaggcgtcac atttgcgttt ggtaacgtta ttagtgctat
    18781 cattacattt ctgatcactg gttttgtctt attcttagtt gtcaaagcgg caaaccgtgc
    18841 gaagaattta cgctccaaag aagagcaagt ggaagtggaa gaaactccta ctgcagaaga
    18901 ttatcttagt gacatacgcg atcttcttgc caaacaagtg gcagaagaca atacacatcc
    18961 agtagagcaa acaaatcttt ccgatacttc taaataataa agaatctaaa aaaaagaaag
    19021 ccgattttca gtgtcggctt tcttttttta gattcttaac ttgctacttg ttcctttttc
    19081 atgtcgatat gtggaatatc atcttccaaa tacacatctg agattgcctt gaatccaaac
    19141 gactcataaa aagcttgtag gtattcttgt gcgccgatca cgattggttt ttctggaaaa
    19201 tttttctgga tcatgttaat cgtttcttga acaatctttc ttcctaaacc atctttacgg
    19261 ttgtcctgat gaacaaggac acgtccaaaa tggatactct ccgcttcttc atatactcgc
    19321 gtgtaggcaa ggatcttccc atcagtatct tgaaaaaacg tatgccaagc ttgtccatca
    19381 atgtcatcaa cttcttgata aggacactgt tgttccacaa caaaaactgc gattcgttct
    19441 ttgacgatcg cgtaaaactc tgctgttgac aattcttcaa atttctttac ctgatattcc
    19501 actaagcaaa cgccccctgc ttttttattc ttagcatacc gattcgcaca caaaaaagca
    19561 aacgaacctt acgaatc
//
LOCUS       DAYYKN010000049        19507 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000049 DAYYKN010000000
VERSION     DAYYKN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.049"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACHBB7_001421"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACHBB7_001421"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM9209380.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACHBB7_001422"
     CDS             complement(1173..1868)
                     /locus_tag="ACHBB7_001422"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM9209381.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACHBB7_001423"
     CDS             complement(1881..2597)
                     /locus_tag="ACHBB7_001423"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM9209382.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACHBB7_001424"
     CDS             2927..3532
                     /locus_tag="ACHBB7_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM9209383.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="ACHBB7_001425"
     CDS             3857..4180
                     /locus_tag="ACHBB7_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM9209384.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="ACHBB7_001426"
     CDS             4362..5732
                     /locus_tag="ACHBB7_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM9209385.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="ACHBB7_001427"
     CDS             complement(5862..7163)
                     /locus_tag="ACHBB7_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM9209386.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="ACHBB7_001428"
     CDS             complement(7393..9384)
                     /locus_tag="ACHBB7_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM9209387.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDKVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="ACHBB7_001429"
     CDS             complement(9528..9794)
                     /locus_tag="ACHBB7_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9209388.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACHBB7_001430"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACHBB7_001430"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM9209389.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACHBB7_001431"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACHBB7_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM9209390.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /gene="def"
                     /locus_tag="ACHBB7_001432"
     CDS             13138..13701
                     /gene="def"
                     /locus_tag="ACHBB7_001432"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9209391.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="ACHBB7_001433"
     CDS             complement(13954..14475)
                     /locus_tag="ACHBB7_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM9209392.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="ACHBB7_001434"
     CDS             complement(14478..15320)
                     /locus_tag="ACHBB7_001434"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM9209393.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="ACHBB7_001435"
     CDS             complement(15313..16038)
                     /locus_tag="ACHBB7_001435"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM9209394.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="ACHBB7_001436"
     CDS             16214..16510
                     /locus_tag="ACHBB7_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209395.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16766..16940
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17078..18670
                     /locus_tag="ACHBB7_001437"
     CDS             17078..18670
                     /locus_tag="ACHBB7_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9209396.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="ACHBB7_001438"
     CDS             18683..19423
                     /locus_tag="ACHBB7_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9209397.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4001 c         3702 g         6224 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt tatcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaa
//
LOCUS       DAYYKN010000050        18732 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000050 DAYYKN010000000
VERSION     DAYYKN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.050"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..978
                     /locus_tag="ACHBB7_001439"
     CDS             82..978
                     /locus_tag="ACHBB7_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM9209398.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1128..2069)
                     /locus_tag="ACHBB7_001440"
     CDS             complement(1128..2069)
                     /locus_tag="ACHBB7_001440"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9209399.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2195..3316
                     /locus_tag="ACHBB7_001441"
     CDS             2195..3316
                     /locus_tag="ACHBB7_001441"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9209400.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3574..5076
                     /gene="eat(A)"
                     /locus_tag="ACHBB7_001442"
     CDS             3574..5076
                     /gene="eat(A)"
                     /locus_tag="ACHBB7_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM9209401.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5143..5670
                     /locus_tag="ACHBB7_001443"
     CDS             5143..5670
                     /locus_tag="ACHBB7_001443"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9209402.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5881..6942
                     /locus_tag="ACHBB7_001444"
     CDS             5881..6942
                     /locus_tag="ACHBB7_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM9209403.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6945..7841
                     /gene="murQ"
                     /locus_tag="ACHBB7_001445"
     CDS             6945..7841
                     /gene="murQ"
                     /locus_tag="ACHBB7_001445"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM9209404.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7855..9837
                     /locus_tag="ACHBB7_001446"
     CDS             7855..9837
                     /locus_tag="ACHBB7_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM9209405.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9902..10348)
                     /gene="nrdI"
                     /locus_tag="ACHBB7_001447"
     CDS             complement(9902..10348)
                     /gene="nrdI"
                     /locus_tag="ACHBB7_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM9209406.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10559..11983
                     /locus_tag="ACHBB7_001448"
     CDS             10559..11983
                     /locus_tag="ACHBB7_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM9209407.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12164..12937)
                     /locus_tag="ACHBB7_001449"
     CDS             complement(12164..12937)
                     /locus_tag="ACHBB7_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9209408.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12934..13839)
                     /locus_tag="ACHBB7_001450"
     CDS             complement(12934..13839)
                     /locus_tag="ACHBB7_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209409.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13993..14313)
                     /locus_tag="ACHBB7_001451"
     CDS             complement(13993..14313)
                     /locus_tag="ACHBB7_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM9209410.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14787..15473
                     /locus_tag="ACHBB7_001452"
     CDS             14787..15473
                     /locus_tag="ACHBB7_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM9209411.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15475..16419
                     /locus_tag="ACHBB7_001453"
     CDS             15475..16419
                     /locus_tag="ACHBB7_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM9209412.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            16618..17262
                     /gene="pcp"
                     /locus_tag="ACHBB7_001454"
     CDS             16618..17262
                     /gene="pcp"
                     /locus_tag="ACHBB7_001454"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM9209413.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            17341..18618
                     /locus_tag="ACHBB7_001455"
     CDS             17341..18618
                     /locus_tag="ACHBB7_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209414.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6001 a         3307 c         3994 g         5430 t
ORIGIN      
        1 tataggaaac gttttactgg ctttttacta tttaaacagg ctatcatagg aaaaagtgac
       61 agagaaagtg aggcggaaaa gatgaacaaa aatgacgtac tgctagttgc aaaaaataaa
      121 tcactttatt ttttgccttc catccgctct ttagatgttt ggtccggaaa agatttacta
      181 cagtcggaat tcattaaatc agctagacaa cagttggcaa attatcagct gatcgttttt
      241 ttagattatg gatttgatat ggagatggct cgacgcgtgc gcccatttac caatgcaaaa
      301 attgtgttat ttttttggaa tcacttcaaa gcagaacacg aggcgatgct gaagcaggca
      361 caagatgaac cggcaatcga tgagatttat cattttgatt ttttggaagc aaaagaactt
      421 gggctgaaac ataatagttc attttattcg aaacatatga atttttcttc tagcgaacca
      481 aagacggatc tattttttgg tgcaacgaat aatgggagaa aagaacgagc agaaagctat
      541 aagcaggaat ttataaaaag agatgttacg accaactatt tcattcttcc acatcgaggg
      601 aatgaacaac ctggatatct tacttatgaa gagtacctga aaaaaacagg ggaatcgcgc
      661 gggattctgg aactgttgcg ggagggtcag caaggagtga ctttgcggac atttgaatcc
      721 atgtattttc aaaagaaact agtgacggat aatcaagcga ttgctcatta tcatttttat
      781 cgtcctgaaa atattttttt gcttcgagaa cgtgatttag atgaattacc ggaatttctg
      841 catacaccat ttaagccaat cctatcagag ctattggaat tttatgatgc agaaaattgg
      901 gcaaaacgat ttatcactaa cgattccact atttatgagc aatacgaata tgacctctcc
      961 tctgttgaaa acaaataaaa aacctacatt ttgaagagat tatgtactga aacaaacaag
     1021 cacaattctt atggcatcat gagagttgtg ttttcttttt gatcttttat tgggcttaaa
     1081 aaaagagaac cagctgctga ttcagctgat tctcatcaga gagcctttta ttctgcgcca
     1141 aggattcctt cttttgcatg gactaattgg gtcaaaaagg cacaatatgg tgtagcaata
     1201 tcatattttt gtccttttcg ccaaatggca ccattgatgt agtcgatttc agtcaaacga
     1261 tggtttttga tcaggtcttg gtacatagat gggtaatgca acccgatgtt gtcaggatca
     1321 taagtagaag caatatgttg gtacacttct tcttgatcta gtatgattcc ttcttttgct
     1381 gcgatagaag caaattcaga tacgattgtt cgaaccatgc tctctgccgc tttttgcgcg
     1441 cctagttcag caatattaca atccaaaatc gtgcataaac cattcaatgt tccatttaca
     1501 caagcttttc gccaaatgga ataacgaacg ttatggctat agatcggatt taaatttgct
     1561 tcttgaaaga cttctacaac tttttttgta aatgcttcgc cttctggttc caagttctct
     1621 agttcgattt ctccatcacc taacaatgtg acttttccgg gaccagctag accagctgtc
     1681 cacatcgtga tcccaaataa gatatttttc tttggcacat atttttccag tacatcttca
     1741 tgacctaatc catttagtaa acacaaaaca taagtattgt cagtaataat ggattggatc
     1801 gaacaaaaca tatcatctaa ttgattggct tttgttaatg ctacgatcaa gtccacgtgt
     1861 tcgttgcttt cgatgatttc ttctggtgaa tagattggca gttttgcgac cacttcttta
     1921 ccgttaaagt cagcaatcaa cccatttgta cgaatggctt caatgtgagc aggccaacga
     1981 tcgatcaaga ggacctcatt tccgctttga tgtagcatca atccgattcg gctgcccatt
     2041 gctccggccc cagctattgc gattttcatg ttttttctct cctttattca atgagttccc
     2101 ttttagtata tagaaaaatg gaagaaaata gcattgtttt gtttgaaaaa aatgaaaaaa
     2161 agtgtcttac ttaagaagaa agtgagggat acagatggat attttggcgg caattgattc
     2221 atttaaagga agtgcttctt catttgaact aaatcaggca gttctaaatg gctgggaaga
     2281 tcgtaaagga agaaaaatca atgtgccgat tgcagatggc ggagaaaata ctgcagaagc
     2341 aatctattac gcgctcaggg gaacatggcg taccattgat gggatggatc tcttattccg
     2401 tcagcgaaaa atcagctatt tattgacaac ttggcaagga aaaaacaccg cagtcatcga
     2461 atctgcacaa gcattggggt tagatctact tgataaacca acggatcaaa ctatccgact
     2521 tgcttccagt tatgccttag gagaacttat taaagatgca ctgcttcaag aagtagaaca
     2581 aattattgtc actttaggag gaagcgggtg tagcgatggt ggtctaggat tactccaatc
     2641 cttgggtgct tctttagaag ggtacacaga aggaaatcct ttattatcta ctaaaaaact
     2701 tcattttgat cgaagaaacg ttcttgtttc taaagtaaaa ttgattatcg ctgcagatgt
     2761 taccaatcca tatacaggtc aacaaggagc tgcttggcaa tttggaagac aaaaaggagg
     2821 aatagatacg acgctagctt tcttagataa acaagcagga aagattgctc agcaaataaa
     2881 agatttctgg gaggtggatt tagatgatat tccaggaagt ggagcagcag gcggtattgg
     2941 tggagccctt tttttgttgg gagcgaagat ggtttctggt ttcgatttag tcagccaaat
     3001 cactggattg gaaaaaaaga tcgaacaagc agatgtcatc attacaggag aagggcggat
     3061 cgatcagcaa acgatccacg gaaaagttcc atacggtgta gcagtgcttg caaaaaaata
     3121 tcgaaaacca ataatcgcta tttgcggaag tagagaccaa gagctagaag gactttctga
     3181 ttttctccca attgtcttaa gtattcattt gggtccaata ggtttagaag aagcaatgga
     3241 acacaggcaa acattagaaa aaacaaaaat cttaggacag acacttagca gatttttcac
     3301 catcctcccg aagtagttga caaaatcatt ttctttatgc tatcgtaatt tcaaatatag
     3361 gacaaagtga gggatccgtg cattgaaaaa ttaagtcgat tgatgaaagc tgcattcaga
     3421 ctgtaccttg tggagacaag gaagcagtgt gcagattttc gggattggct tggttttttc
     3481 gatgtatggg tctttttgtg tgtatccaac ccttgcatcc tctttagcca gttctgaaaa
     3541 taggactggt ctttttttac gaggtgatgg aaaatgtcta aaatcgaaat aaaaaatctg
     3601 acattcggct acgacagcca aggcacatta ttatttgaac aagcaaatct aaattttgac
     3661 acacaatgga aactaggact tatcggacga aacggtcgag gaaagacaac tttactgaat
     3721 attctacaaa acaaactacc ttatcaaggg caagtaatcc atcagcaaga atttgcctat
     3781 ttcccgcaac agacaaaaga taaagaacgt ttaacctatt acgtgttaaa tgatattacg
     3841 gattttgaga tatgggaaat cgaaagagag ctccaattga tgcaaacaga tcctgaaatc
     3901 ttatggagag aattcagcac actatcgggg ggagagaaga caaaagtcct actggcactt
     3961 ttatttgtgg atgacactca tttcccgtta atcgatgaac caacgaatca tttggatatc
     4021 tctggtagaa aacaagtagc ggcttatttg aaaaagaaaa aacaaggctt catcgtggtc
     4081 agccatgacc ggggatttat cgatgaagta gtggaccatg ttttagcaat cgaaaaaagt
     4141 caactggaac tttatcaagg gaatttctct atctatgaag aacagaaaaa acttcgtgat
     4201 gaatttgaaa tggctcaaaa tgaaaaattg aaaaaagaag tcagtaggct aaagaaaaca
     4261 gcagctgaaa aagccgaatg gtctcgttcc cgagaaggag ataaaacaaa gaaacaagtc
     4321 ggattcatcg atactgaatc tagacgagtg aataaaggag cagtgggtgc tgatgctgca
     4381 cggacgatga aacgatccaa agcaatcgtg aatcggatgg agacccagat cagcgagaaa
     4441 gaaaaactat taaaagatat cgaatatatc gattctttga cgatgaatag ccaagcgtct
     4501 caccataagc gacttttaag cgtagaagat cttcaattag ggtatgaaaa tctgttattc
     4561 gagccaattc attttacaat cgagcctcat cagcgggtgg cgatttcagg tcctaacggt
     4621 gcaggaaagt catccattat ccattatctt ctgggggcat tcaacggcaa ggttatagga
     4681 gaaaaatacc agccaaaaca tctgagcatt agttatgcaa gccaaaatta tgaagacaat
     4741 cgaggaacgt tggcggaatt tgcagagaaa aaccaagtag actaccaagc atttttgaac
     4801 aacctccgaa agcttgggat ggaaagagat gtttttcata acaagatcga gcagatgagt
     4861 atgggccaac ggaaaaaagt ggaattggct aaatctttat cacagccagc tgaactatat
     4921 acatgggatg aaccattgaa ttatttggat gtcttcaatc aagaacaatt agaacaactg
     4981 atcttgaacg tgaaacctgc catgttacta gtggaacatg atcaaacctt cctggataaa
     5041 gtatctactg agattatttc tcttgagaga atctaaccct tgtaatctca agaacaacaa
     5101 ggtatggtta aggggaactt tgccaacgaa ggagagaatc acatgcttta tgtaacgaga
     5161 cacggagaaa caacttggaa cgcacaagga ttagtttgcg gacgggcaga tgtgccgctg
     5221 actgaaaaag gacagatgca ggcacagaaa ctagcagaaa aggtagtaga tttgcctgtt
     5281 ccaatcacga aaatcatcca ttcccctctt caacgagcaa gagatacagc acaagcagtt
     5341 gctgatcgtc tcagcttgcc gctgaccgtt gatgaacgat tggtagaaat ggattttggg
     5401 gattatgatg gactgccaag caaagatgag aactttcaaa aagcacgttt agcgttcgct
     5461 gtacgattcc caaatgggga gtcagtctta gatgtgtatg cacggatcgt tcctttattg
     5521 aaagaatgca tcgaagacga agaaaatgtc tatctgttag tttgccataa tgccttgata
     5581 agagtgatca atgcttattt tcatccgatg cctaatgagg ggttctttac attcatggta
     5641 gataatacag aattggtttc ttatgagtaa ttatgttttt gtgttattta aataaaatat
     5701 ttcaaggcac tgcttttgct tagcagtgcc ttttttaggt tatgacaatt cttttgataa
     5761 tataaactca acctttagaa atgaaattta attattttaa taaaagtatt gaaattttgt
     5821 ttctttagat atatactcta gtcatagaaa tgaagacgga tacagaaaaa gagggataat
     5881 atgtttggct tttcaatatt tatgaacgat tctttgtcag aagagaagaa acagtatatc
     5941 cgccaaatgg catccaatgg ctttgtcggt attttcactt ccatgcatat accagaagat
     6001 gatgtttcgg catatcaaaa gcgattgatc gaactaggaa gcgaagcgaa aaagaataaa
     6061 ctagaattga tggtcgatat ttcaggagag gcattggaac gagcgggttt ttcattagaa
     6121 gatctaacgc ctttgaaagc aattggtgta acaggtttaa gaatggatta tcatatttcc
     6181 aatcagcaga ttgccgactg gtcacatcaa ttgaaaatca gtttgaatgc tagcacgatc
     6241 acgcctaaag atatcgatga attgaaagaa gcagaagctg atttctcaca gattgaagct
     6301 tggcataatt attatccacg tcccgaaaca gggctggaca aagaatggta tcagaaaaaa
     6361 aatcaatggc tcaaaattca aggtctcctt gtccaaggtt ttgtaccagg agatacagag
     6421 ttgagaggcc cactatatca aggattgcca actttggaag aacatcgagg gatccatcct
     6481 cttgcaggcg ctctagatct attagcaagt aacacggata ttgtttacat cggagatgca
     6541 ggtctgtcag aaaacgttca ggagcaattt gcttctttcc aaaaagaaca gacagtcttg
     6601 ctccatacgg aacctgtaga tgaagaattt tacgaatata tattagggaa gcatacgaat
     6661 cggcaagacg atgcaagaga tgtcatacga agcgtagatg cacgttttcg agagattccg
     6721 cctatcccag caagaaatac agctacgcgt atgaaagggt ctattacttt agataacgaa
     6781 aagtatttgc gctatatggg tgagatccaa ttaaccaaat atgatttgcc agcggatgaa
     6841 aaagtgaatg tagtagctaa agttatcaaa gaagagctac cattgatcaa ccaaatcaaa
     6901 gctggtatga actatcaatt tataagaaaa gaaggaagat aaaaatgagc aaaatcaatt
     6961 tagagaacct aacaacggaa cgcagaaacc aagaaacttt tggattagat gagatgagta
     7021 tttctcaagc cgttcaacta atgaacaaag aagatcagaa agtggccgct gcggttgaaa
     7081 aagaactgac aagtatcgaa ccagtgattg ctaacacgat tgagtccttt aaaaaaggtg
     7141 gacgtctgat ttatatggga gccggaacaa gtggaagact aggtgtgtta gatgctgctg
     7201 aatgcgtacc gacattcggt gtagaaccgg aaatggtcat aggtctaatt gctggtggag
     7261 aagaagcaat gactgtagct gtcgaagggg ccgaagattc ttatgagcta gggcaagaag
     7321 atctaatcaa gctgaatttg acaaaaaatg atatagttat tggtatcgca gctagcggtc
     7381 gtacaccata tgtgatcggt ggattagatt acgcaaaatc gatcggtgct gtcacaggaa
     7441 cgatttcttg caataaacaa gccgaaatca gtaaacacgc ggatttccca atcgaagtcg
     7501 atgcaggtcc agaatttttg acaggctcta ctcgattgaa gtcaggaaca gcacaaaaac
     7561 ttatcttgaa tatgatttct actatcagta tgattggtat aggtaaagta ttcaataact
     7621 tgatggtcga tgtcaaacca acaaatgaaa aactaatcga acgctcaaaa cgaatcatca
     7681 tgcaagcgac agaagctgat tacgaaacag ctgaaaagta ctttgaagaa gcagatcaaa
     7741 atgttaagct ggctatcgtc atgattttga caaatagtga taaagaggaa gctcaagcaa
     7801 aattagctga agcaaacgga tttgtaaaag ggaccttatg agggggaaaa gaccatggca
     7861 aaaaacgaac tgactttgga agaactggca aaacaaattt ataaaggggc aggcggcatg
     7921 ggcaatgtag aaagcattgt ccactgcatg actcgtgtgc gtatgaagat catcaatgat
     7981 gatcttgtaa atgtctctga attaaaagcg attccaggag tgcttggtgt agtagaagat
     8041 gaacagcttc aagtcatcat cggacctggg aaagtgaaca aagtagcaca gcaaatggtt
     8101 gatttggcag gcgtgaaatt aggagaaaaa ctttcttctg taagtacagc tacagcaaat
     8161 tcatcagcat ctggaaaaga ccgtgtaaac gaacgggcac aagaaatgaa agcggcacaa
     8221 aaatcgaaac aaaaaccatc taaaatcaaa tcggtattaa aagacatttc taatatcttt
     8281 gtgccaatga ttcctgcatt tgtcggtact gggatcgtgg cagggattgc cgcagtttta
     8341 gcaaacttag tcacagccgg aacacttgat gcagcaacat ggcaacaata tatcgatgtg
     8401 atgaacatct tgaaaaacgg gatgtttagc tacctagtta tctatacagg tatcaatgct
     8461 gcacaagttt ttggtgcaac accaactctt ggtggagtta tcggggccgt tgtgatgctg
     8521 acagggatga atccggaagc accactgaaa aacttattta ctggtgaagc tcttgcagct
     8581 ggacaaggtg ggatcctagg tgtcatcttc gctgtttggc tattgtcaat cgttgagaaa
     8641 aaactgcata aagtagtacc tgatgcggta gatatcatcg ttacaccaac tctttcattg
     8701 attgccatcg gtctgattga aattttcttc atcatgcctt tagcaggttt catttctgat
     8761 ggcatggtcg gtgggatcaa ctgggtactt ggcgttggtg gagcatttgc cggcttcgtt
     8821 ctaggcacat tattcttgcc gatggttatg ttcggactgc atcaaatttt gactccaatc
     8881 catgtgcaaa tgattgatga aactggtcgt acgctattgc ttccaattct tgcgatggct
     8941 ggtgcaggac aagtgggtgc agcattggca ttatggattc gctgcaaaaa agataaagaa
     9001 ttaacagaaa tgatcaaagg agctttacca gtcgggatcc taggaatcgg cgaaccattg
     9061 atttacggag taacattacc tctaggacgc ccatttatta ctgcttgtat cggtggaggt
     9121 atcggcgggg cagtgatcgg aatgatcgga aatgtcgggg ctatcgctat tggaccttca
     9181 ggagccgctt tgatcccatt gatctctgac ggtaaatggt taggctacat ccttggatta
     9241 ttggcagctt atgctggtgg cttcgttgct acattcttct tcggtattcc taaagaccgt
     9301 ttagaaaaag aagaattagc gactgaaaca gccacagtaa atgaagtgcc tgaaaaagta
     9361 gtacctcaaa cttcagctga aagtgtgact atcctctcat cagttgctga cggaacagtt
     9421 gtttctttag ataaagcaag tgatccagtt tttgcacaaa agatgatggg tgaaggctac
     9481 tttgtagaac cagaaaacgg acatatctat tctccagtta ctggggtagt cagctctgtc
     9541 ttcccaacga aacacgctat tgggatcaca acgccaaatg gtttagaaat cttgcttcat
     9601 atgggaatca acacagttga cttaggcggt acgccatttg atgtgaaagt aacagaaggg
     9661 cagcaagtga cacctgatac gcttgtagca gaagctgact tagaagcgat caaagcagca
     9721 ggcaaagaaa catccatgat cgtcttggta accaatatgg atcgtgtaaa aaactttgtc
     9781 ttggaaaaaa caggtaaagc aaaagcaaaa gcacctgtga tggatgtcaa agcttaatat
     9841 gatagaaaca atagaaacga aagaagaaac tgaaatctat ttttcagctt cttctttttt
     9901 attatttatt gttttctgct aaaatctgat aaattcgtgt cacatcagat ggtgttcccc
     9961 gtaattcata atctgccaag aaaggaaagc caaatgtttg tgcatattgt ttggctgtta
    10021 gacaatactg attattgaag tttttatttc cgctaccgac tacaccaaga caaagtttat
    10081 gattgtcttc atgttccaga tattcacgca tcgtttcggt caagatttct gtatcaccgt
    10141 tatctaaacc atttccgccg tctaaataag taggaacgaa agtaaaaaat ggttctgttt
    10201 ctttcgtaaa atcactattt tcatggattt cttttaaagt aacttcagga ttgacaggat
    10261 cttctgcatg cattttttca gcatattctg ctagatgttt ggcaaaggca cgcgtatttc
    10321 ctgaaatcga tatatataaa atgttcatgg atcatcctcc aattctttcc ttttagtata
    10381 gcatcttttt taagtttttt ggtggtgtaa tttggaaagg gactaggcgc tttgcttgta
    10441 taatgagaaa aaagccggta gaattatatt tctattaaag tttttattaa ttaagtaaaa
    10501 ataaaaaagg tttgttatac tttttgataa aagcggtttt attttggagg atgcttacat
    10561 ggatacagag caacagaaaa aaggaattgg tttaattcca cttgctgcct tagtaattgg
    10621 ttcagcgatt ggaggtggcg tatttggtat tatgacggac atctccggat cggctggcgg
    10681 cccagctatt ttcagttggg tactcgtagg aacagcaatg ttgatgctct ccttgtcgat
    10741 caataatatc aataaaaaac gtccagattt aaaaggcggg atctttagtt acgcagaaga
    10801 aggttttggc cgtttcgctg gttttatcag cggttggggt tactggctca catgctggtt
    10861 aggcaacgta gcttttgcaa cattgttgat gagcgcatta ggatatttct tcccagtatt
    10921 tgaaggtggg caaaatctgg catcgatcgt gatgagtaca gtctttttat ggggatatgc
    10981 atggctagtg aatcggggag tcgaaaatgc tagtttcgtc aatgctatcg tcacattctg
    11041 taaattagtg ccattatttt tatttattgt agtagctatc agcacgtttt cgatccaaca
    11101 attcattgat aacttttcta gcgacattat attgaatgcg aatggacagc cgatcccgtt
    11161 ttccagtcaa gtattgagtt gcatcatggt gatgctttgg gtatttgttg gtattgaagg
    11221 agcctctgtc gtaggttcac gagcaaaaaa acaatcagat gttgggaaag caacgattct
    11281 agggatattc ggcttgatta ttatttacgt cttggtttcc atgcttccat atgggacaat
    11341 gacgatgaat cagctgcaaa cgatcaaggc gcagcctgct atgggctatg tgtttgaaat
    11401 gatggttggt aaatggggag cagtcgtgat caacggcggt ttgatcattt ctttagttgg
    11461 tgtttggctc tcatggacga tcttaccgat tgaaacaatg ggaaacatgg ctgaagacgg
    11521 acttttgcct aaagcatggg gaaaactcaa caaaaatggg gcaccaactt atgctatcgt
    11581 attgacgacg ttatgtacga atgtcttttt actttcatta ttattcactg acagtgctta
    11641 taactttgct tacacacttg gtacagcagc tattttcttc acgtggcttt tcttaggatt
    11701 gtaccaaatg aaactttctt atcagcggaa agaatgggtc caatttttag tagggctttt
    11761 agcaagtgcc tttcaagtat gggctatgct agtcgtagcc tttaatgaag taatgcttgt
    11821 tcttgttctt ttcttgccag gcatttattt ttacctcaaa gcacgtaagg agcaaaacga
    11881 gcctgtacaa catatccact tagataagat tttacttata ggtgtgacga tggtcggagt
    11941 gattgcaatg gttttatttg ccactgggat gcttcacgta tagaagtata aaagaagtat
    12001 aaagagaggt tgtgagcctg tcgtttattc aaaaacatac taggaaagga gctgtgacac
    12061 aaatgttgtc acagctcctt tttcagaata aacggtgttt aagagacagc caatcggcaa
    12121 taaggctgaa cgtctctttc acagcctctt ttttgatgag tcatcataca ttttttcgct
    12181 taaatacgag atatcctaat gctaagaaca aaacgatgta agctaaatta ccgaatagca
    12241 tttgattagt agaaagatga gtcaattgct tcatgatctc ttcgtttcca atttggtttt
    12301 gcagattcaa catactgatc ggattccatt tgatccactc ccatttttgg atgagtgcaa
    12361 taaggatacc agatatgatt gacgaagcaa aataaaagac gattcctgct gagatggatg
    12421 cgccggaact attgataaag caagcaatca tcaacactaa acttaaaatc agccataaac
    12481 cgatataact acccaaagta taagtgaaca agctttggat cagtgtttga ccagtagaaa
    12541 ccttctccgt gaaggaaatc gaagggaaaa gaatcagttt catcaacaca gtgacaataa
    12601 tagctaaaag gtataaataa acagaataaa taactaatgt gatccatttg cttactaata
    12661 tttctcctcg agaatacttt cgataaagga gatttttcaa tgtaccatat tgtgcttcca
    12721 tcgaaatgat cgtgcttgca gctgcaatca tgataaagac aatccaagat gtggcactaa
    12781 aaagctgagc agtcatttca actggatcaa tgatcgtcgt gtattttttg gctagaagtg
    12841 ctgttcctat gagcaaaaca actaatagaa cagataaaac agcagttgac tttttcttga
    12901 tcaatttaaa taattcttgt tttacaagca atgtcatttt tcttcttccc cctttactga
    12961 atccagaatt tccaatagag aagattccaa atcattcgtc agatgctgga cgtctgtcac
    13021 atggatattt tgtgcagaca atagctggat cacggttcct aataattctt cgttttcttt
    13081 taaaagcttg attttatcag gatcttccaa agtatatcca gcttctgtca agatcgttcg
    13141 agcagctaga tcatttgttg tagagataac gatgaaatct tgcgctgttt ggttcaattc
    13201 agccattgta cattgtttga tgatctttcc ttgattgatc aggatcagct catcgaccat
    13261 tttttcaagc tcgctgagta agtgactgga gatcaagaaa gaggtgccct gagtagccaa
    13321 gtcggtaatg atttttcgca catctcttgt tgcttgtgga tccaaaccgt tcatcggctc
    13381 atctaaaata acgatagacg gatggttcag taaggcaaga gcaattccca atttttgttt
    13441 cattcccaac gagtaggatt tcgctttttt gtgaatgtac ttttccatct tgagctgatt
    13501 cacgatagtg tcgatagcag atacatcgtt ggtttcagag aataacttca gatgatcata
    13561 gcctgttaag aatggataga tacccgggta ttcgatcaat gctcctactt gttccaaccc
    13621 tttatgacta gagggagaaa tctcattttc accaatacag attgttccat ctgagtaatg
    13681 gatcagtcct aaaatagctt tcatgatcgt tgtttttcca gcgccattag gacctactaa
    13741 tccaacgatt ttgccagggg ataagctaaa actgacatcg ctgagggctt gtttgtgacc
    13801 aaagttcttg gataattctt ggaccgaaag acttttcaaa aatattcact ccttttctat
    13861 agatagtaca agcatacacc tgatgagtgg agaagtcgct aggtcattgg acttatttct
    13921 tattttttca taaaaaaatt gaatattaaa taaaaaggct tattttttct gatcagtact
    13981 tattccaagt gtctagtggc cgctagtaat cttgatgcca atgatactga tgagtagcat
    14041 ccccacaaag aaccaagtgg aaagattcag cttatcatga aacaagacca caccaattat
    14101 gaccgaacca actgcgccaa tccctgtcca gataggataa gagagactca tcggcaaact
    14161 tttgattgcc tttgacaaaa agtaaaaact agcaatcatg ccaatcactg tgtaaaaaga
    14221 ataattgatt ttagaaaaac catcactcca tttcatcatc gttgaccaga atacttccaa
    14281 aattcctgct atgattaatt ccatccatgc catacttaca ttcctcctta actataaaaa
    14341 aatacacaaa aagaggaaaa tcatcccttc aaagacctaa tggaatgatc atcctcttta
    14401 tcctacccgg tggcagattg tatatctttt tctgtagtat agcacaccta tccagatatc
    14461 ggcaatctca tactttttag taaagtgtac caagcactga cggatatatc gttagcggaa
    14521 aagaagatga atgctttgca atttcataga aagaagttgt tatttattaa tcatttaaaa
    14581 aaaacagtaa ctagacctta tttctatata ttatcgggca ataaattaaa aaaagcttta
    14641 ccttacaata agtaagataa tttttgtgat aaaaaaataa cgaaaaatat ttaaaaatag
    14701 tgtctatttc gtgagaaaac aactatattt tttatgtgtt ttgctttata attaaaaaaa
    14761 tattgtcatt ttatggagga aaatatatgg aaaattatct tagcctttta ggcgtcttga
    14821 ttgttattct cgggtttgca tttaaattag actcgatatt gatcgtaatg gttgctttag
    14881 ttgttacagc tttgactggc ggtcttggca ttcaaggaat gttagaactt ttaggtacaa
    14941 gttttgtaaa caatcgcggg atggcgattt ttatcatcat tatgttggca acaggaacgc
    15001 ttgaaagaaa tggtctgaaa gaatcagcgg cttccttgat caagcgattc aagaaagtat
    15061 ctgctggtat gattgtagat atttatggga tctttcgaat gatctttgcg gcttttaacg
    15121 tgagttttgg tggagttgct ggatttgttc gtccaatcat tttaccgatg gcattgggga
    15181 ccatcgaatc aaaagggtta gaagtccatc ctaagcatga agaagaacta aaaggtatgg
    15241 cttcagctat ggaaaatgtc tgctggttct ttggccaagt actattcatc ggaggagcag
    15301 gggcgttgct agtccaatca acactgaaag atctaggata tgacgtaaca ttgggacagc
    15361 ttgctttagt ggaagttcca gtggctatca cggctttagt tgtggcaagt gtctacttct
    15421 atcttaaaga aaagaaatta gcgaagaaat attatccagg aaaggggcaa taagatggac
    15481 ttttttacaa atcctgatat tttattaggc gataaattac tggagatcct ttatatcgtg
    15541 atgggactcg tattgattta cactggagta agaaatttaa cagataaaac caatcctcat
    15601 cgttatgggt cagccttttt ctggacggtc ttaggggtag ttattgcagg aggaagatgg
    15661 cttccggcaa tcgtgaacgg tggcttgatc tttgcgatga ccataccagc aattgctaag
    15721 aaagtcagca aaggagaaag ccggcttcca tcaaaagaat atatggaaaa aatgtcagac
    15781 aaactaggaa tgaaaatttt tattccagct ttgagtatcg gtgtctttgc gatcttgttt
    15841 gctctattta ccaatttagg tgctttagtc ggcgtgggcg tcggtgtttt tgcagccatg
    15901 ctaatcttga tgttcttttc tcgagataat cgaccaagta catttttaga tgatgcagca
    15961 gatatgctgg gcacagtagg gccgttaagc atgttgccaa tgcttttagc ttctctaggt
    16021 gcggtattta caagcgctgg agttgggaca gtgatttcca atgcagtagg tacgatcgtt
    16081 ccagaaggaa atgtgaacat cggcatcatc atttacgctc ttggcatggt gatatttacg
    16141 gtaatcatgg ggaatgcatt tgcggccatc accgtgatga cggttgggat cggtgcacca
    16201 ttcgtattta gtcatggagc gaatccagcg ttaataggaa tggttgcatt gacttgcggt
    16261 ttttgtggga ccctgctgac accgatggct gctaacttca atatcgtgcc tgtagcgatg
    16321 ctggagatga aagacaaata cggagtgatc aaaaaccagt tgtttattgc gttatttatg
    16381 ctagttttcc aaattgctta tatgattcta tttaaataat agaagagagg ggttgtgaca
    16441 gaataattgt cgcaacctct tctaacattg tagtggaaca ctattttttt ataagaatat
    16501 attaagtttg atctgttatg ttatttaaac tattgtttcc tttatttacg ctgttcaagg
    16561 aaaatggtat gataaaagat aaccatatgt taaatcaatc ggacggagga acaacagatg
    16621 aaagtactag taacaggttt tgatccattt ggtggggaca aagtgaatcc agcctatgaa
    16681 gcagtaaaaa aaatgccaga tgaaatcagc ggtgctgaaa tcatcaaagt ggaagtaccg
    16741 accgtattcg aaaaaagcag tcaagtagtc aaagaagcca tccaacagca ccaaccagat
    16801 gtcgtgatct gtgtcggaca agcaggtggg cggtcagcag tctcgttcga acgtgtcgca
    16861 atcaatctag cagaagcaag aatccctgat aatgaaggaa atcagccatt cgatacggct
    16921 ttagaagaaa acggtccagc cgcttacttt acctcattgc caatcaaagc aatgacaaaa
    16981 aatgtacaag atcatggtct accagcgtac atctcttata cagcaggaac atttgtctgc
    17041 aatgatatca tgtatcgttt gcttcatttg atcgaaacac aattcccggc aattcgaggt
    17101 gggttcatcc acgtgccatt ctcacccgat caagtgatcg atcgtccagt gggtacgcct
    17161 tctatgtctt tagaagacat tgcttcttct ttgacttatg cagttgaagc agcagtagaa
    17221 aataaagaag atatttccgg aaatgccgga acaactcact aatttagcaa gaaacactgg
    17281 ctggttgcta acgttatgtt agtagccagc tttttgtaat tagaaaggaa agaaacagcc
    17341 gtggaagaat ggcaatccgt ctttgaagaa tggtttccaa aagaaatcag caaaagctat
    17401 cccatcaaaa tttctaaaca atacacaagc agtcaacgtt gggagatcta tgcaaaatta
    17461 acgaaaaaac agcgggaact agtcgataag caccgccgat atctaatcag ctctcgtttt
    17521 atggaagaac attatttagc tgcaacagat tgggtgtttt ctgacttcaa gatcaatccg
    17581 ttttttcgaa caaaacgaag ccagcaaaaa ttgtattgtg agtgtggtag agaattaaaa
    17641 gtgcaataca tcgttaaatc gccaaaaacc ggaaagatcc taaaactagg catcaaccac
    17701 tttgccgatc acctgcatgt ctctccgaca gtggcagctt cgatccatca gggaatgaca
    17761 aaagttgatc tggccttaga cgaattatta tggcttaagc agaagaatat cgattttcct
    17821 gaaggactat ggcaaaaata ctgttttgtc ttgtaccaaa atcgtcggat gaaacagcca
    17881 tatttaccgg atataaaatt agctcagcgg ctcgcagaat ttcgccaagt agaaatgccg
    17941 atttacatag cggattatca agcattggaa aatgaaatca agaaaataag tgaacacatc
    18001 aatgggcagt caaaaaagcg acagataaaa aaagaactat ttgacgactt tgcagaagaa
    18061 ttagtaaagg atgtagaaga attcttgatc aactatcgcg catttttacg taaagattgg
    18121 caatccatcg tatatgaaga agtgccagta catcctaatg cttattttga aacatttatt
    18181 tccgtgttga gaaagacaaa acgtcaacga acaccagaag tcactgctca gatggaatat
    18241 tttgcgaaaa accaacgatt catccaacca aaaatctatc tatttatttg gaagcaatat
    18301 tgtcgctatg gttttacaga agggttcttc gacagcattc ctcggatagt cagaaatggt
    18361 tttttaaaag tattgcgtaa agaacgagaa gctatacaat ctgctgataa gaaagaccgt
    18421 acagtctcaa aagaaaaatg gcaactagta gtgaaagata tccaatctgg aaatgtgcaa
    18481 gagacaatcg acaaatggaa aggaaaacat tatcgtttta ctgaagctca gaaacaagcg
    18541 ttggaatatt atcagaagtt agaagaatca ctgcgtttca atgatgaggc aagaaagtat
    18601 ctgaaggaat tactataaaa aacatactaa gattagaggg gaacatcatt gacgaatgat
    18661 gttctcctct attttctatt tttagattat agtgaagagt gaaagaaagt tcgaacaaag
    18721 ccttgaggac ta
//
LOCUS       DAYYKN010000051        18504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000051 DAYYKN010000000
VERSION     DAYYKN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.051"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACHBB7_001456"
     CDS             99..746
                     /locus_tag="ACHBB7_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM9209415.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACHBB7_001457"
     CDS             1139..3784
                     /locus_tag="ACHBB7_001457"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM9209416.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACHBB7_001458"
     CDS             4094..5407
                     /locus_tag="ACHBB7_001458"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM9209417.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACHBB7_001459"
     CDS             5397..6050
                     /locus_tag="ACHBB7_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9209418.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACHBB7_001460"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACHBB7_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM9209419.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACHBB7_001461"
     CDS             6947..7147
                     /locus_tag="ACHBB7_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9209420.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACHBB7_001462"
     CDS             complement(7401..9080)
                     /locus_tag="ACHBB7_001462"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9209421.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACHBB7_001463"
     CDS             complement(9082..9294)
                     /locus_tag="ACHBB7_001463"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM9209422.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACHBB7_001464"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACHBB7_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM9209423.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACHBB7_001465"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACHBB7_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM9209424.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACHBB7_001466"
     CDS             13269..13466
                     /locus_tag="ACHBB7_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209425.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACHBB7_001467"
     CDS             13619..14104
                     /locus_tag="ACHBB7_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM9209426.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACHBB7_001468"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACHBB7_001468"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM9209427.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACHBB7_001469"
     CDS             14981..15508
                     /locus_tag="ACHBB7_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209428.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACHBB7_001470"
     CDS             15599..16336
                     /locus_tag="ACHBB7_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM9209429.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACHBB7_001471"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACHBB7_001471"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM9209430.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACHBB7_001472"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACHBB7_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM9209431.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3200 c         4022 g         5298 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAYYKN010000052        17642 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000052 DAYYKN010000000
VERSION     DAYYKN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.052"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..942
                     /gene="sgrA"
                     /locus_tag="ACHBB7_001473"
     CDS             <1..942
                     /gene="sgrA"
                     /locus_tag="ACHBB7_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM9209432.1"
                     /translation="LGLSIIAAMIGISTSANERANENLSFTVKTDRIVYDMTQQVITI
                     PVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEYTIMISAELSID
                     EIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESSTENTSGESSTES
                     TSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMSSESSTGSTSSE
                     SSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSD
                     LPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            1240..1596
                     /locus_tag="ACHBB7_001474"
     CDS             1240..1596
                     /locus_tag="ACHBB7_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209433.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            1641..2756
                     /locus_tag="ACHBB7_001475"
     CDS             1641..2756
                     /locus_tag="ACHBB7_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209434.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(2782..3222)
                     /locus_tag="ACHBB7_001476"
     CDS             complement(2782..3222)
                     /locus_tag="ACHBB7_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9209435.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            3382..3888
                     /locus_tag="ACHBB7_001477"
     CDS             3382..3888
                     /locus_tag="ACHBB7_001477"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9209436.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4056..5969
                     /locus_tag="ACHBB7_001478"
     CDS             4056..5969
                     /locus_tag="ACHBB7_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9209437.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            5974..6975
                     /locus_tag="ACHBB7_001479"
     CDS             5974..6975
                     /locus_tag="ACHBB7_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9209438.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(7177..7383)
                     /locus_tag="ACHBB7_001480"
     CDS             complement(7177..7383)
                     /locus_tag="ACHBB7_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM9209439.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(8029..8877)
                     /locus_tag="ACHBB7_001481"
     CDS             complement(8029..8877)
                     /locus_tag="ACHBB7_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9209440.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            9041..9943
                     /gene="gnd"
                     /locus_tag="ACHBB7_001482"
     CDS             9041..9943
                     /gene="gnd"
                     /locus_tag="ACHBB7_001482"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM9209441.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            9945..10946
                     /locus_tag="ACHBB7_001483"
     CDS             9945..10946
                     /locus_tag="ACHBB7_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM9209442.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFNVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMCEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            10943..11227
                     /locus_tag="ACHBB7_001484"
     CDS             10943..11227
                     /locus_tag="ACHBB7_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM9209443.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            11260..12057
                     /locus_tag="ACHBB7_001485"
     CDS             11260..12057
                     /locus_tag="ACHBB7_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9209444.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLSSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            12071..12904
                     /locus_tag="ACHBB7_001486"
     CDS             12071..12904
                     /locus_tag="ACHBB7_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9209445.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            12922..13779
                     /locus_tag="ACHBB7_001487"
     CDS             12922..13779
                     /locus_tag="ACHBB7_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9209446.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            13801..14310
                     /locus_tag="ACHBB7_001488"
     CDS             13801..14310
                     /locus_tag="ACHBB7_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9209447.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            14347..14748
                     /locus_tag="ACHBB7_001489"
     CDS             14347..14748
                     /locus_tag="ACHBB7_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209448.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            14860..15213
                     /locus_tag="ACHBB7_001490"
     CDS             14860..15213
                     /locus_tag="ACHBB7_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209449.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            15247..15690
                     /locus_tag="ACHBB7_001491"
     CDS             15247..15690
                     /locus_tag="ACHBB7_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9209450.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            15707..16792
                     /gene="uxuA"
                     /locus_tag="ACHBB7_001492"
     CDS             15707..16792
                     /gene="uxuA"
                     /locus_tag="ACHBB7_001492"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9209451.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            16785..17498
                     /locus_tag="ACHBB7_001493"
     CDS             16785..17498
                     /locus_tag="ACHBB7_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9209452.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         6169 a         2724 c         3745 g         5004 t
ORIGIN      
        1 cttgggctga gtataattgc agcaatgatt ggaatcagta cttctgcaaa tgaacgggca
       61 aatgagaatc tttcatttac tgtgaaaact gatcggattg tttatgacat gacacaacaa
      121 gtcattacta ttccagtcaa accaaacaaa agcgtgaatg ctagtgatgt acatgctgtt
      181 ctaacatatg gttgggatgg taatggaagt tcagaaaaag ttattgggga agtttattta
      241 aaagacgtac aatggaccgc tggtattgag tatacaataa tgatttctgc agaactatcg
      301 attgatgaga tcaagagtaa agataaagtt gatttgattg ttttttatga tggacagatg
      361 acgatcacgg agaatttgaa accaagcagt tggacagttg ttggaccgcc aagcagtacg
      421 gatagcacaa gtagtgaaag cagtacagaa aacacgagcg gtgaaagcag tacagagagt
      481 acgagcagtg aaagtagcac agaaaacacg agcagcgaaa gcagtacgga gagtacgagt
      541 agtgaaagta gcacaggaag cacgagcagc gaaagcagta cggagagtac gagtagtgaa
      601 agtagcacag gaagcatgag tagtgagagt agcacaggaa gcacgagcag cgaaagcagc
      661 acaggaaaag taaataatga acttgatttg gtacctatca taccaactaa ggaacaaaag
      721 agaaatgcag ggagcaacct aaaaagtgtg ctaaaacagc ctgattttgt ttctcgtgga
      781 attgatcatc aagaaaaagc gaaagaaaca gataatagaa aaagcgattt accagaaact
      841 ggtagtagaa ccttgaacaa gctaattaca tggatgggtg tgttactcat tttgattgta
      901 ggagcaagct attttcgttc tctcttccat cgagtgaaat aaagaataaa agcaacagat
      961 ctaaaattga ataaaacgat agatttttga gtgaatattt cattcgagag tgctgtattg
     1021 cctggcgaag ttgtagcttt gccagatcta tttttgttta ctaacatatt tgagaaacag
     1081 gaaataacag gaacacaaat ggaaaataag agcaagaata attgacaatg cacactgcgc
     1141 atggtagtat ctctgaaaaa tcagtaacga atctatttgt acgttattca tgtagctgtc
     1201 ggaataagtc taaataatag ataaaaagga gtatgtactt tgtttgaaga atttattgat
     1261 atcaatgaaa ggcaagtata tcaatttttg aactattgtt atgaaagaga cgagaaatta
     1321 tatgtggtga aggatatcgc tttagatcta aactacaccc tggctaagat gaattcggtg
     1381 attcaacaag cagaatcgtt ttgcgaaagg tatccagaat ataaattatc ttttctttca
     1441 gaaaacaaga tgataaaagt cgagttttct tctcagtttt tactgagtaa agtttattct
     1501 atacttttag agggaacgat tggttatatt ttactggatt cactttacaa aggtacttat
     1561 caatccttag aaaatcttag tcaaaaaatt atataagttt gcgtacgatt caaagaaaat
     1621 taagagaact taatactatt ttgaaaaact atcatttatc ttgcagcttg aaaaaaggta
     1681 atccgataga aggagaagaa taccgtatcc gatacttttt tcacttgatg tattggcagt
     1741 cattcgacga aaatcattct gatatattat caagtataaa aaataaacgt caactattag
     1801 aaaaatttgc gaaacatgcg ccatatacgc gtagtataga tagaaagaaa tggatcagcc
     1861 tactaagaat ttcgttgcaa cgaatgaaac aaggccactc gattacgtat gtcccagaag
     1921 agatgaacaa atttgtgcat ccattgataa caaaagaaga gtttacgcat tacatactag
     1981 agcctttttt tgaagcaaac ttctggtcga tgaatcacat cactccaaca gagcaagctt
     2041 acctttattt tatgtttggt gtaatgaaca cctatttgga agaagatttg ttggatactc
     2101 cagataaagt acaagaaagt tggggctgga ttgaatcgtc tattgtagaa aattttgaaa
     2161 aaaacttcaa gatttgtttt acaacttcag aaaaaaatta tctatatgtc aatttggcaa
     2221 tgatccattt gtattcaagg gtttttggga caaaaaagaa ggtggatgct tttggaaaat
     2281 cagcagaaga cagagatttt cagcatatat ttccagtgat gtatccaatc atgcaacagt
     2341 tttttagtgg ggtagctcgc agaattccag agcttaatac ttattatgaa aagaacagac
     2401 ggttgatttt tcaatactgt atgttagtca gggatatttt tataaaacac gaacaacctg
     2461 tcatattcta tattcaatca aagtatggta aaacacagga attatgggca aagaaaagga
     2521 tattatctat gactgaacgc ccaataaaat atagtgcatc cgctgatcag ttacctgata
     2581 ttgtcattag cgattatcct atcgacaaaa aggcatatga tcaaacgaat acacagatat
     2641 tttactggaa tggacaacct acaaaaaatg attgggcgcg tctgaaagct gaaatcataa
     2701 aaatcaggaa tgagaaaaat agatccatat tggaaaaatt ggatttcgtt aattaagaaa
     2761 agcagattgt ttatgccaaa attaagacat tgcaaaaaga ttattttctt taaaatcgac
     2821 aagatgacca gaaagcaaga gttcgttgag ctcttgcttt cttttagttt tagaaaggaa
     2881 catcctgatt ttatcttcgc taaatccagt caaaagtttt gcagattggt cgtagataat
     2941 cggttttttc aataaagaag gggatgtacg aatctcaata tatagttcac tcaaactgag
     3001 ttcatctatt ctgtgaacaa gatttttata actaatagaa tgagtagaga gcatttcggt
     3061 cacgccgttg tttgtcaatt ttagtaagtg gataaattca tttttggtta gtggttgacg
     3121 aattaagttc tgttcgataa acggtatatc atggacgata agccaacgtt ttgcgcgtct
     3181 tgaagacgag cagccagaac ctgtatacaa tttaatcatc atacattcct ccaatagcta
     3241 agtatttatg aaataataac atgaagaaat agtcgaaaag tgccatttag aggtgtaaaa
     3301 taagaaaatg ttgtcacgta taatggaact agctagataa gaaatactat taaatcaaga
     3361 aaaaagaaag aaggcacaag tatgtcaaac acagccgacg caatagtagt gatcgattta
     3421 caaaaagggg tatgttttga ttcttcgccg atacacgaat tagaaacagt gacagcatta
     3481 gtgaataaaa gaatcaaaac atacgaaacg attgggaaac cagttatttt cgttcaacac
     3541 gaagatgctc aattagtgcc acaaacaaaa ccatgggagc tattgtcgga attatctatt
     3601 cctgagcaca gttattttgt gagaaaaacc catgcgaatt cattttataa gactaatttg
     3661 agtgttttat taactgaatt ggactgtcat tcgatcgaat tatgtggggc acaagcagag
     3721 tattgtatag atacgactgt gaagtttgct catggattag gataccagct tatgatgtgt
     3781 agaggagcaa cgacaactta tgataatcct tttatgaaag caaaggaaac aatcgctttt
     3841 tacgagaaaa tttgggataa acgatttctg acatttttgg agcaataagt tgtttcttcg
     3901 tttaaaaaaa gcagaatgga tactggttta tccattctgc tttattacgt aaaatgagaa
     3961 aataatcctg ttctattttt atgggataaa ccatttattt tttgtataaa aaatgtttgc
     4021 gtttacatat aattctccta gaaacataaa aaggagtgaa tagcttgaga acaaaacaag
     4081 tattaaaggg agcgttggtc atcttatttg ctgtaagtac aagcgtcccg caatggacag
     4141 tatatggaga gagcatttct tccgaatcgg aatctcggga ggtagaaacc acagaaacta
     4201 ctgatgctgg aaatgaaacg aaatcgagta cttcacaaac agaaacaaat actgaaacaa
     4261 cggttgagac atctgttact agcacagatc aagaaacgac cgctaccaca gataaccaac
     4321 aaacagaaga agcaacagct acaaaaatgt caaacgacga atcctcttct gctaaagtaa
     4381 gtgacaaaga gaatgggaca gaaaatgatg atatcggtaa aggtgaaaat ttcaatggta
     4441 aagaatattt gaattacttc ggtaaacaga tgacagaaat acttaatgga ccaacagaag
     4501 acatcaagtc aaaattaata gagttattag tcactgagct tgctgttaat gattatggtg
     4561 tagataaaga gattatcaaa catctgattg ctacttatct attgtacgat atcgcttttg
     4621 caaaaaacag tgtgttgatg tcgatgcttg gaggtacaga tgatgggaca acgcctacag
     4681 aaaacacagg cttctatcca aatacaaaac aacttcatca aactgtacta aacaaacaag
     4741 atggcaaaat gatggatgta tatgcatatt atgtcgatca aggttcagat aaaacagtca
     4801 tgatccatgg cggatttcga gggaattgga ataacgggat cgttacagaa gaatacaatg
     4861 atttttataa ggccgggtat aatttattat ttgttgattc cagagcgact ggtaatagcg
     4921 gaggcgatta tgtgacctat ggacaatatg agtcagatga cgtcctttac tggataaatc
     4981 aagaagttcg agagcggcca tctcaaaaga ttttacttta tggtggttcg atgggagcag
     5041 ctacgatgat gtctgtatta gcgaaagata ttcctgtcaa tgtcaaagga attattgaaa
     5101 attgcggatt tgcatcaatt gatgaacaac tacgttttac gtacagccag acagtagccc
     5161 ccgcactacc ggatgccata aaaaatcagt tagacatcat tggggatcaa gagcatgaag
     5221 atttatttat gggccttttg aaacaatact attttgatca ggagatgcat ttggatacaa
     5281 aagctgctct acctacgatt ggaatgtcag attcattgcc taaattgatt atccatggaa
     5341 cagcagatga tgtagtacct gtgtctaacg cgcaaaaatt atatgagtta tcaggagggt
     5401 ataaagatct tttattagta gaaggagcag ggcatggaaa ggcacaggaa gttgaccatg
     5461 ctgcctacac gaaacatgta acggactttt tgaaagtagt gttccatgat cagataaacg
     5521 taaagtatgt ggatgaaaac aataaaagtc tactcaaaga ccaggacgaa atccagcttt
     5581 acggagctta tggagaaaat tatatgacag agcaaaagac attcgaagga tacgaattag
     5641 caaatgttga aggaccgact gaagggattt ttaatgagac gataccaact attattttct
     5701 attacaaaaa aatcccagta gctccaccaa aaaagcaaga tccgacagag aatgcagaca
     5761 ataagggaaa gaatgaacct cgagaggatc aatcaagtga tgatacgcaa ccgattcaga
     5821 tgacagtgaa agtacaaaaa aagacagatg caaaaaaaag aatgttgccg aaaactggag
     5881 aaaagaaaca ttcgcttctc atttattcag gtggattttt atctgttagc acgattatca
     5941 catggatttg gcgaagaaag agagggtaaa tgaatgaaat ggttcaagcg aatagtgatt
     6001 gcagtaccag ttatcatcgt agttatttta gtcactgtat ttttgattaa tcaaatcaca
     6061 ccaaagccga ttagcttgct tgttagaaaa caatttgaca caagtgaaaa agaacaagta
     6121 tatgcccgac cagatgattt ccagcaaaaa atcgaacaaa tcgatataaa aaaagaaata
     6181 tcctatcctt caaagtacaa aaataataat atggatatct acacaccgaa agtggaaaag
     6241 aaaaaattac cgattctttt ctggatgcat ggaggcgcat atgtaggagg ggataaaaat
     6301 gattgtcgtg attatttgga atatctatgt tcagacacgc agcaaatcat cgtaaatata
     6361 gattatgaac gatcaccgga agcaaaacat cctacaccag ttatccaatt gaacgaagcc
     6421 attcaagcag ctaaaaaaga gataaaagat caagcagatt ggtcgaatat ttcgattggt
     6481 ggaaattcgg caggtgcaca aattagtgga gaatatttgt tagcacttca aaatgaagaa
     6541 attaaaaaag ctgatcatct tgatccgact ttggagaaca agcagattac gaagtttatt
     6601 tctttgtctg gtctgttgga tccttctgca cttactcaag taagtgataa aatctctagc
     6661 tttctttatg aaaaatgtgg atgggcttac tttgataata aggactttga acaatcagag
     6721 aaggtcaaaa gtttagcctt ggtcagacat gcggatcgtt ggacgcaaaa cacattctta
     6781 acggatggaa atacaaatac gtttacgaaa caaatggaag aagtaacttc tgtatttgag
     6841 aaagcggggg tgaaagtcac aaaagtagat tatgcggaga aaaatgctgt tttgaaccat
     6901 gaatatcagt ttgatttcag caataaacaa gcgcaagaaa catatcaaaa gttagtaatg
     6961 tttttcaagg agtaaagtct tatgatgcaa aagaggttgt aatctgtcac ttagctccaa
     7021 gtcacaagga gcaatttcaa taaacaagct tttcatggtt tatgaatatt gtaacttgtg
     7081 actgaggagt tagagatggc aaggggcacc ttttattcga aatttgggaa tatcacacga
     7141 ttattgtcac gatctctttt acgtaaattt tataagtcag atttgttgtt ttgatcgttt
     7201 aaaaaaagca acaaatccga agcctgcgga ggcaagaagg gacaaaaagc cgagtggtaa
     7261 gcaaaagaat gctggttcca ctaatattcc gttgttattg atataaggct gggaacaaag
     7321 atgaatagca aataaaatca atgcactagc gaaaaaaagc aaagaaatat ggatagcttt
     7381 cataagcagc acctcctttt ttataattgt atgataaata gattaagaat ctataaatgt
     7441 gacaagtttg ttaggatgtg aataaaatga tcatctagcg aacttgaaac aaagatggct
     7501 aatgataagc aattaatgat tcagcaatca gataggtaca aagtttacca tcatacgtct
     7561 agaccaataa tagaaaatat gggaatataa tagaaaagat ttaggatacc cccaaaagaa
     7621 tttccccaaa aattcgatgt atcctaaact tttttatttt acataacaat catatcaact
     7681 gtgaaataaa attacaaatg ataacctaaa atgaggccaa aagccagtgt tatcaaggaa
     7741 atacttaatt caccaaaatt ttttttaatt ttttagaaaa taagaaacaa aaataataaa
     7801 aaaaagaaaa gataaatttt ttactactgt atactaactt tgttatcgca tctaacaagt
     7861 aactcgaata ataccaatac aaccttctcc aaagatgcga tgacatacgt tctaacccac
     7921 cgactattga tgttctgtac cacccttgca gaaccaaaaa agccactacc aaatatttgg
     7981 tagtggcttt ttttgtttta tggatttttc gctttttctg ttatggtgct attctttgat
     8041 ttcagaaata gtatgtcgaa ctttagcgat agaacgacgt gattcttttt cgttgtaaaa
     8101 catcaaaatc acatatagag agtccaaaat gcttagttga gaaatacggg aagctaaagc
     8161 ttcagatcgg aattctgttt cttcagaaat agaaataaaa acgacatcgc caagtttagc
     8221 aagggaagaa ttagcttggc tagtaataac gattgttttt gctccagttt ttcttacggt
     8281 ttcagcaata tggattgttt cttttgattg ccctgtgtga gaaatcaaaa cagcacagtc
     8341 tttttccgtc aataaagaag cttccatcaa ttgaatatgg taatccgtgc tgtgagaagt
     8401 tgcgataggg gaacgcaaaa atttatgata agcgtcagtt gctacaatct ctgaaccgcc
     8461 aacaccgaaa aaatagacac gtttagattc attgataata gtagccgctt tttttagatc
     8521 cttttgttgt aacaactttt ttgtgtcatt taaagttgaa atactagact cgaacacctt
     8581 ttgcgccata gtgagctcag tatctgtttt ctgaatattt tcgtggatag aaatagcaga
     8641 tagatcattt tcttggatca acatggccat tttaaaatct ttaaatccat tataaccaag
     8701 tttttttgta aattgaaaaa aagtagagtc agcaatgccc agttcacttg ctaaattgct
     8761 aataggacta tgacttactt tgcttggatt ttctaaaata taatcggcga tagcctgttc
     8821 ttttgggctg aagtcgttgt acattgtttt gatttgtagt cttatggaga cgctcatgag
     8881 aattcctcat tttcataata agattatcat tttttccgtt ctcattaatg ttaaagatac
     8941 aaggagagta ggaaaaaatc aaacaaaaaa tatttttgta aaatctattt acaaaaaaat
     9001 ttttttgtat aatgagggta taagaaatgg aggcgctatc atggaaattg gtgtaattgg
     9061 tttgggaaaa atgggattga atttagcttt aaacttaaaa gagcaccatc acaaagtaca
     9121 aggactcgac atttcaagta cagctgttag tcaggcaaga gaaaatgaca ttcctgctta
     9181 tcaagagatc tctgaatttc tagctacttt tgagcagaaa aaaatcatct ggttaatgct
     9241 tcctgctgga gagataaccg aaaacatgtt aagaaaactt tttccaaaac tcggaccagg
     9301 agacattatt attgaaggcg gaaactctta ttataaagat tcaatccgtc gagcagaaga
     9361 gtttcagaaa aatgaaatcg gttacttgga ttgtgggact tctggaggaa tcgaaggagc
     9421 acgacatcaa gcctgcttaa tgattggagg agataaagaa acttttctat ccgtggaaca
     9481 attattcaaa gatgtagcaa ttgaaagagg atatctgtat gctggtgcat ccggaagcgg
     9541 acacttcttg aaaatgatcc ataatggaat cgaatatgga atgatgcaag caatcggtga
     9601 aggattccaa cttgtacaaa aaagtgagta tgcttttgac ttggaacaag tcgcacgtgt
     9661 ttggaatcat ggttcggtga ttcgcggctg gctgatggaa atcatggaag atcagttaaa
     9721 cgaacatccc aatttaagta attatcgggg aatcgtttct gcatcaggtg aagcaaaatg
     9781 gacggttgaa acagcgctag aaatggctgt tccagttcca gtaattgcaa ctagtttgtt
     9841 catgaggaat ctttcacagg aagaagatag tttttcagca aaagtagttt cagcactaag
     9901 aaatgggttt ggcggacatg aaatagttga aaagggtgag taaaatgcca gattatcata
     9961 tagcaatcgt taattccagc agtttcggaa gagtctttcc acaccacttg gaaagattaa
    10021 agtcagttgg agacgttcat ttctttaatg ttgatcaaga gattccagga aaagaactag
    10081 cggagttact taaaggattc aatatcatta ttgcaagtgt tacgccattt tttcgacgag
    10141 atttttttga aaacaaagat gaactgctat tgatttcccg acatggtatt ggttacaaca
    10201 atattgattt aaaagcagca aaagaacacc agacaatcgt atctatcata cctgctttag
    10261 ttgaacgaaa tgctgtagca gaaaataata taactaattt gttgtccatg cttcgacgca
    10321 cgacacaagc acaagcaaga gtcaaggacg atcgatggga agatcgggcg gagttcgtcg
    10381 gtaggacgct gtttaacaaa acagttggtg tgattggtgc aggaaatacc ggaagcggag
    10441 taatcgaaat tttgcgtaac ggttttcgct gtgatgttct agcttatgat ccttacaaat
    10501 cagatttata catcgaaagc tttggtgcaa aaaaagtaga acttcaagaa ttactggaac
    10561 aatcagatat catttgttta tgtgcgaatt tgacagaaga gaactatcac atgatttctg
    10621 ataaagaagt cgatcagatg aaagaaaatg tttatctatc taatagtgca agaggcgctc
    10681 tagttgatga agaagcgatg attaatggtc tgaaaagcgg aaagatcgca ggatatgcca
    10741 cagatgtttt agaagaagaa ccgggacgtc aagatcatcc gtatttagcg tttgaacaag
    10801 tagtgattac gccacatata tcggcctaca caagggaatg tttagaagca atgtgtgaaa
    10861 aatgtgtcag agatgtcgaa cagatcagtc agcaccttct tcctgaacgt tctgtccaga
    10921 caaagagcaa ctatataaaa ggatgatgaa gaaaatggtg aacggtttaa aagtgaaaac
    10981 aggccctcaa ttttatttat atgaagaagg tggtatttca aaagtttctg atctcttgaa
    11041 atcatacggc gcgaaaagag tactggtcac ccacggaacg gtatcatggg aaaaggcgct
    11101 acctaaatta gtatttttga atgacgaaac gattcaattt ttttatcatc gttattctgg
    11161 agaatgtagt tatgctgaag ccagacgaat agctacaatc atcaaaaaaa tgaaatcgat
    11221 ttcgtagttg gcgttggcgg aggaaaactg accgatctag tgggttatag tgccaatctt
    11281 gcaagaatcg atttcggagt catccctact ttagctagta attgtgctcc atggacacct
    11341 cttgctgtca tgtataagga gaatggacaa gcagaaggga aaacagaaca cttttacaga
    11401 caagctgctt tttttataac agatccccaa ttagtgattg attcccctgt tccatatttc
    11461 attgccgggt tagcagatac actagcaaaa tggtatgaat ctgagctcat cttagaacaa
    11521 gattttttgc aagatgaatc atttttgaag ctagcacaag atactgcgaa gatctgcaaa
    11581 gaagagattt tgaatcattc tgctaaagca atccaagata tggcacaaag aaaactgtca
    11641 tcggagttca aacagctttc agagattatc tttgctgttg ctggtttagt tggaggatta
    11701 ggagataaat acgcaagaaa tgtggcagca catgctatgc atgacgctat cagtaaatat
    11761 attccggaaa gccatcgttt tctacatgga gaaaaagtag cgtatgggat tttctatcaa
    11821 ctggcactag aaggtcgctg ggaaaaaata gacgagttat atcctttgta tcgggaatta
    11881 gaactgccgg tttctctaga acaaatggga ttatttccga aagataaaca gataatagat
    11941 aaaatggctg aatttattga ttcgaaagag aaagtgcatt tgattcctat agaggtaaca
    12001 cagaaatcac tgatacagtc aatgatggaa ctggaaggtt atatcagaaa gatctgaagg
    12061 aggaggacac atggaaacat taagtgtagt acaaagtttg ttgattgctt tatgggtagc
    12121 cgcggttatg tcccgctggt taggtggagg agccacgttg accttacgtt tttctccttt
    12181 gatgactgga ttagtcgtcg ggttgatcat gggggaagtt gcacaagcga tgattgttac
    12241 agccgctttg caaatgattt atatgggggt tttttctcca ggaggatcaa tgccagctga
    12301 accatctatt gcagcagcta tcgcagtacc agtcgcatta ttaggggatc tgtcgccaga
    12361 agcagcgatt gccgttgcgg tgccagtcgg attgctcgga agttacttgt atcagtttcg
    12421 ttttttcatc aatacttttt taggtaaata tacagacaaa gctgtagaag atttgaatac
    12481 aaaagctatg gcccgatcaa ttattcttta tccaacggtt gcctcatttt tattattcgt
    12541 tccgcttgtt ttcattgctt tatatttagg ggcgccattg atcgctgatg taatcaaagc
    12601 tttagaggga acagtcgtta tccacattct tgaagtagta ggtggagggt tagcggcaat
    12661 cggtatcgcg acaactgtat acgttatcgg tcgaaaagat tatctggtct ttttcttcct
    12721 tgcttatttt atgagtgttg ttttgaaatc tttagatatt acgatggtta cttatgccat
    12781 ttttgggatt attatcgctt tgatctttgt ccagtcgcaa aaagggaaac aagtacagcc
    12841 tgccacaagc aataacaatt cagcatcttt tgatgacgaa gatgactatg atgacggatt
    12901 ttaatttagg aggaatggga aatggctgaa ataatggata atcaaacagg tgctcctgaa
    12961 gaaatcacga aaaaagatgt aacaaaagcc tatctgagat ggcattttgc aaacgaaatt
    13021 cctcactctt ttgaacgtta cttggcgcca tcactactat acggaatgat gccaatcttg
    13081 aaaaaacttt ataaagatga agactcttta aaagcagcat ataaacggca gttactgttc
    13141 ttcaacactc aattaagctg gggtggcggt gtgattactg gcctaatgtc ttctatggaa
    13201 cagcaaagag cagaagaaga gtatgaagaa aaagagatct tgatgcagga tgacttgatg
    13261 tataacacga aagcgggatt gatgggggca ctagcaggaa tcggagatgc aatcgattca
    13321 ggaaccgtcc aatatatttt tatcgcaatc gcggtacctt gggcacaaca gggaagtcct
    13381 ttaggcgcac tttttccttt tatctgcttt gggttgtatc aggttttact aggggtattt
    13441 ttcgcaagac aagctttcac tatgggccgt aatgctacag gcctaaagca aaacactggt
    13501 gttcagacag cgattgaaat gctatcagtt ttagggttgt ttatgatggg ggtgcttgcg
    13561 ggaaactatg taaaagtaga atcttcattg aaatttgcga tatccggacg tgagtttgtt
    13621 atccaggaaa ttcttgatca aatcgtgcca gggatattac ctttagctgt cgtaatgggt
    13681 gtgtacttgt tctatacgaa aaaaggattg aaagtcacac aagcattatt atggctgact
    13741 ggaatattaa ttgtgctagc aggaatcggt attttataaa aagaagggaa cgtgaataag
    13801 atgggtgtag tcaatttagc gcgtgtggat gagcgtttga tccatgggca agtaatggta
    13861 acgttatctc aaaaaagtgg agtgaattcc atttttgttg tagacgaagt tgtagcaaaa
    13921 gataaattta tgagagatct ttacaaaaat gcaggaagcc gtacaggaca aaaaacaatt
    13981 gtcattacac cagagaaagc taaattctat tgggatgaat atcaatttaa agaatacaat
    14041 tgtattttga tcgcaaaaac agtccaaacg atttacgact tagtaaaaca cggaatcccc
    14101 atggaagaat tgaatattgg agggattgct caaaaaaatc ctgatacaga tcttttagtg
    14161 acgaaatctg tttatttaaa taaatcagat gctgaaaaat tgaaagaaat gaatgaacaa
    14221 tatggcgtaa agaatattta ttttcaagcc actccatctg cacctaaaac aggattaaaa
    14281 gatgtattga acaagtttga tttgtcataa acaaatgctt agaaaaaaga taaagagggc
    14341 gaaaaaatga agaagaacga gctgtttcgt gattgggaat ttcgttatcg ctatatttac
    14401 cgtaagcgcc gtacaaaaaa gagtaaacag cgttttcttt cagcattagt atcagatatt
    14461 tacagtatga gaacagatgt gacagtcatt gcttacgata cgctagccta ccgatcaaaa
    14521 aatatttatg ttggagatat cgaaaaagca gagaaagtta tctgtactta ttacgataca
    14581 cccgttcatg cattaggaag ttatttcatg tttgattgga aagaccaaag gaaaaaaaca
    14641 atctactcta tattattgtc cttcattttg ttgttcagtt taggttggtg gggaatgatg
    14701 atatataaca aaaatcctca tcacgtattc gatctgctat ccgtataaac aggtatcact
    14761 gtactagcat ttgggagcta ctttttctta ttgggaaaag cagctcgcgg atggcctagc
    14821 agacaaactt ttattcgaaa tacttcatcc atattaacga tgctagaaat gatccgtacg
    14881 attgatgatc cgagtgtagc gtatgcgttt gttgatgaag gctgttatgg agaaaagggc
    14941 ttagacagcg tgagatcggg tatgaaaaaa gaggctattc ttttttatct agactctgtt
    15001 ggggcagata caccgctaca gttttcggga aattattttt caaataaaga acagtggctg
    15061 aaacaagttg ataagttgaa agaaaaaaac gtcaattata tttttagtgc aagaaaaaag
    15121 caagcgcagt tcttctattt gaccaaaaca gatttacgag ggaaaacatt taattggcaa
    15181 aatgccaatc agattattgc tctttttcga taagtaaaaa gtgcaaaaca aggagtgaga
    15241 agactaatgt tggaaatcat gatcgttaca catggtaagt tgagcgaagg actgaaagat
    15301 gcggcggaag taattgtcgg gacatcgagc catattgaaa caatgtgctt agagtctggg
    15361 gaggacatcc aaacattcgg agagaaggtc aaagaaaaat taactatgat caacacagca
    15421 gaacccatgc tagtatttgt tgatctaatt agcgcaagtc cgtacaatca agcgctcttg
    15481 gcagtcaatg aactgactga agaaaagaaa gcagccactt atgtcatagg gggcgtgaat
    15541 ctgccaatgt tgttagaagc aatcaatcat cagatgcttc acacaccgat agaagaagcg
    15601 gtggaacaaa ttgtcaaaca aggaaaagat aatttaactg tttggcatgc ttcagcggaa
    15661 gaaaaaaaat tagatgagga cgatttttag aaggaaggct gaaaagatga agtggggatt
    15721 tagatggtat ggtgaaaaag gggattcgat cccattaaaa tatgttcgcc aaataccggg
    15781 aataaatggg ataattggaa ctttgctgac taaaatgcct ggagatgttt gggagatttc
    15841 agaaattgag gagttgaaag catctgttga gagccaagga ctggaactgc ttggaattga
    15901 aagtgtagcg atccacgatg caatcaaggc agggacagat gaacgagatt actatattga
    15961 taactatata cgaacaatca aaaacttgtc tcgatgtggg gttcgattga tttgttatag
    16021 cttcaaacca atttttggat gggcgaaaac tgatttgttt aatgaaaaca acgatggaag
    16081 ctattctctt ttatacgatc agaaaatcgt cgatcagatg gagccaggag aaatgtatca
    16141 attgatacac agccagtcaa agggattcaa attgccgggt tgggaagaag aacggttgaa
    16201 aaaattcgct cagctggaaa aactgtatga aggaatgacc gaagaagaat tatttgaaaa
    16261 tctgacttat tttttagaaa aagtgattcc agtgtgcgaa aaagcagatg taaaaatggc
    16321 tatccatcca gatgatccgc catgggaact ttttggtttt cctagagtga cgaaaaatct
    16381 tggcgacttg aagaaaattc taaatgcagt agattctcca tataatggac taacgctgtg
    16441 cacaggatct ttaggggcaa atccggaaaa cgatttagtt gaaatcatcc atgaggtagg
    16501 ttcgcgcatc cattttgtcc acttcagaaa cgtggagttt ttaggagaaa gaaagttcaa
    16561 agaaacggca catccctcta cagaaggctc ccttgatatg tatcatatta tgaaagcgtt
    16621 ggtgcaagaa gggttcgacg gtcctatacg ccctgatcat ggtagaaccg tgtggggtga
    16681 agtggctatg cctggatatg gcctgtatga tcgtgcaatg ggactcactt acatgcaagg
    16741 tctatatgag gcgataagta aggaaaaacc actgggagag cattatgaat aaaatcaagc
    16801 tcttcatctt tgatatggat ggccttttat tagaaactgg acgtttagct tatcgtgctt
    16861 atgtaaaatc agcacaaaag tatgattatg aaatgcgtaa ggaagtctat tatttactaa
    16921 ctggccaaac agaaatggcg ataagagaac aaatgggcat tttgtacgga gaagatgttc
    16981 catatatcaa atggcgagaa gcaatcaatc aatataaaga aaaaatcgtg aaagaagata
    17041 agagagtata tacgaaaaaa ggagcagaag aaattttatc ttttgcaaaa gaaagaggaa
    17101 tccatacaat cgtggcttcg tctaatacac gggtaaaagt agaaatgtat ttaaaaatgg
    17161 aaaatctata tgccttgttt gatcaaatca tttcagggga tgatgtaaaa aaaggaaagc
    17221 cggaaccaga gatctttttg aaagcttgtt caaaaatgaa tattcctcca agtgaagcac
    17281 ttgtatttga agactcgatt gcagggatcg aagcagcaag gcgagcgggc attttgtctt
    17341 ttctcatcaa agatcatttg atagggttgc ccgatcataa tgggaagcat aagttgaaag
    17401 tagatgtaag tttatatgaa tccaataaag cagactatga attttatgac ctgcatcaag
    17461 caaaacgatt tttagaaagt aaagggttgt atctctaata aagaggacta ttttctagtc
    17521 ctctttattt attgtcctgc acgactgagg gaacagatga aaaagaagta attttttatt
    17581 ttgaagtatc taccttattt gtagtgaaca atttagaaca aacacaaaac gcatctactg
    17641 tt
//
LOCUS       DAYYKN010000053        17455 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000053 DAYYKN010000000
VERSION     DAYYKN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.053"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..674)
                     /gene="rpsD"
                     /locus_tag="ACHBB7_001494"
     CDS             complement(63..674)
                     /gene="rpsD"
                     /locus_tag="ACHBB7_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM9209453.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            936..1787
                     /locus_tag="ACHBB7_001495"
     CDS             936..1787
                     /locus_tag="ACHBB7_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM9209454.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            1815..2450
                     /locus_tag="ACHBB7_001496"
     CDS             1815..2450
                     /locus_tag="ACHBB7_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM9209455.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            2470..3243
                     /locus_tag="ACHBB7_001497"
     CDS             2470..3243
                     /locus_tag="ACHBB7_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9209456.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            3651..4448
                     /locus_tag="ACHBB7_001498"
     CDS             3651..4448
                     /locus_tag="ACHBB7_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM9209457.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            4426..4839
                     /locus_tag="ACHBB7_001499"
     CDS             4426..4839
                     /locus_tag="ACHBB7_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9209458.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            4823..7468
                     /gene="mutS"
                     /locus_tag="ACHBB7_001500"
     CDS             4823..7468
                     /gene="mutS"
                     /locus_tag="ACHBB7_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM9209459.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            7486..9594
                     /gene="mutL"
                     /locus_tag="ACHBB7_001501"
     CDS             7486..9594
                     /gene="mutL"
                     /locus_tag="ACHBB7_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM9209460.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            9616..10176
                     /locus_tag="ACHBB7_001502"
     CDS             9616..10176
                     /locus_tag="ACHBB7_001502"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM9209461.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(10261..12249)
                     /gene="treP"
                     /locus_tag="ACHBB7_001503"
     CDS             complement(10261..12249)
                     /gene="treP"
                     /locus_tag="ACHBB7_001503"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM9209462.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            12472..13185
                     /gene="treR"
                     /locus_tag="ACHBB7_001504"
     CDS             12472..13185
                     /gene="treR"
                     /locus_tag="ACHBB7_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM9209463.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            13262..13708
                     /gene="msrB"
                     /locus_tag="ACHBB7_001505"
     CDS             13262..13708
                     /gene="msrB"
                     /locus_tag="ACHBB7_001505"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM9209464.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            13879..14481
                     /gene="ruvA"
                     /locus_tag="ACHBB7_001506"
     CDS             13879..14481
                     /gene="ruvA"
                     /locus_tag="ACHBB7_001506"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM9209465.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            14494..15495
                     /gene="ruvB"
                     /locus_tag="ACHBB7_001507"
     CDS             14494..15495
                     /gene="ruvB"
                     /locus_tag="ACHBB7_001507"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM9209466.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            15524..16102
                     /locus_tag="ACHBB7_001508"
     CDS             15524..16102
                     /locus_tag="ACHBB7_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9209467.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            16154..16636
                     /locus_tag="ACHBB7_001509"
     CDS             16154..16636
                     /locus_tag="ACHBB7_001509"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM9209468.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            16753..17127
                     /locus_tag="ACHBB7_001510"
     CDS             16753..17127
                     /locus_tag="ACHBB7_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209469.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         5795 a         3115 c         3822 g         4723 t
ORIGIN      
        1 aaaatataaa aaaagaccag ctatccaata gatagcggtc tttttcaaga aagcttttga
       61 aattataagc tttggttgta gtattcaacg acaagagctt cgtcgatttc tggatatagt
      121 tcgtcacgtt ctggtaaacg agtcaatgaa ccttccaatt tttcagcgtc aaagcttacg
      181 aatgctgggc gtccaacagt tgcttctaca gcttctttta ctgttacgat gttttgtgat
      241 ttttcacgaa ctgagatcac ttgacccact tctacgtggt atgaagggat atcaacgcgt
      301 ttgccatcaa ctaaaatgtg accatggtta actaattgac gtgcttgacg acgagtcgtt
      361 gcaagtccta aacggtaaac aacattgtct aaacgttgtt ctagtaagat catgaagtta
      421 acaccatgtt taccttcttt gattttgcta gcttttacga acaagttacg gaattgacgt
      481 tcgttcattc cgtacatgtg acgaagtttt tgtttttcag ttaattgcat gccgtattct
      541 gattgtttgc cacggctatt aggtccatgt tgacctggtg cgtaagggcg acgtgctaat
      601 tcttttcctg tgcctgacaa tgagatgcct aagcgacgag aaattttcca tgatggtcct
      661 gtataacgag acattaaaat ttcctccaat gaatataagt ttttggagta aaataatccg
      721 ttgaaaaatt cataatcata tagtttgttc ttcaatcttc acctttgcag ccgcggttac
      781 acaatcgaac ccgagacggg gcaacaaact agggatgagt tattattttt ctgctgcatt
      841 attttacaca aattacagta tatgatagat catccacgaa tgtcaagata attagtacat
      901 aaaaacgctt tacaaaccta ggctgtcttt ataaaatgag agggtatgtg caaatggaaa
      961 aaagcgaagg aagtttaagg agaaaatcaa tgaatccggt atcaccatta tttgagaaaa
     1021 tcgatgcttc aattgaaaag aaggcgaatc tttttgaaaa cagcttttca cgttacgctg
     1081 ttcgagcggt tctcgcctgt ttgtttttga ctttaggaac ggctgtggca tttgcgatcg
     1141 cgatgaaggg ggaagatatc gcgcatggtc taggaaagat tttatacgca tttatgttca
     1201 gctggggtct ggtcatgatt ctatacatga acgccgaatt aggaacatcg aatatgcttt
     1261 atatgaccgt gggtgttttt cggcagagac ttgctttaag taaggcagcc aagattctat
     1321 ttgcctgtat ttttttcaat atgattggcg gtatattatg tggatttcta atttctatga
     1381 ctacgccatt tcaagattta acgttggaca actttatgtt agaatcaata gcagtaaaac
     1441 tagcaaaacc ttcagcaacg attttggtgg aagggatgtt tgcgaatatc gttgtcaata
     1501 ccgctgtact gatcagcatg cggatgaaag aggatgtcgg aaaagtgatc acagtcgtat
     1561 tcatcatttt catttttgct tttttaggat atgaacacgt gatagcgaat tttcctgcct
     1621 ttacgcttac ttactttgct tctcacggac agatggatgg aatgacagtc agcaatgtgt
     1681 tacataattt gttttttgct ttggcaggaa attatattgg tggcggttta gtaatgggac
     1741 tgggatatgc ttggctgaat cagtcaaaat catcctatgt ggactaaaaa gtcgttcatt
     1801 aaaggggaag tgccatggaa gttttagttt ttccaaaaaa tgaggaaaaa gcgttacaaa
     1861 tggaaaagta catgaaaaat cttttttcct ttgctggagt acctaagccg gaacgcgctc
     1921 ttttacaaca accgttactg gatgagagca aaaatttatc tatttctgaa gttttagctt
     1981 ttgttcatta ttattatgaa caaatcgaaa gagagtatca gtatgtagcg atcgatttag
     2041 tccagaaaaa catcaaacgg cttacatttg aagacttagt cgtattgttg ccactagttc
     2101 agaaaaaaga atggtgggac agcatcgact cttggagaaa agtatacagc acatggtgta
     2161 aagcctaccc agagcagcgt gaagaagtct ttcaattgtt tgaaaaaaag gaagattttt
     2221 ggctacgtcg aatagcaatc acgttgcaat taggttataa acagcaaaca gacacaggac
     2281 tattgatccg agcaatagac gaagatcgtc aaacaaagga attctttatc caaaaagcaa
     2341 ttggttgggc attacgcgat tatagtaaga ccgatgccga ttgggtaaaa agacagctga
     2401 caacagattt gagtaagtta gctgttcgag aggcaagtaa gtatttataa gaaagcgaga
     2461 cgacaagaga tgagacgcaa attatttgct tttgatattg atggaacgtt gttagattca
     2521 aacaaacaag ctttagatag cacaagagaa gcattggaaa aattacgcaa agccggtcat
     2581 ctggtcacga ttgctacagg gcggagccga tttcatgcgc aagagatcat tcgcgatctt
     2641 gattttacga actacatttt atgtaatgga gcggccggtt ttttagacca tgaacaagta
     2701 tataagaatt tactagatcg cgaccagttg gatgcgttcg ttcaagaagc gcatcatgag
     2761 cagatcgata cagcttttgt cagcttagac aatatcaagc gttcttcttc caatgatgta
     2821 acggtgatgg aagaagcgat gcattctttc ggtgctgtct tgcctgaatt agatcagttt
     2881 tttgcagagg aacaagaaat ataccaagcg ttagcatttt tcgatcaaac atatgatggg
     2941 aagttctctt catatgataa aattcgattt gtccgctggc atgagaacag tgtagatgtc
     3001 gtacctaaaa atggttctaa agcagcgaca attctgcact tagcagaaca agtaggcatc
     3061 cctcatgaag acatcatcag ctttggcgat ggacaaaacg accgtgaaat gttgcgcatg
     3121 tcaggaattg ggatagctat ggggaatgct gtgccagaag tacaagcaga agctaagatg
     3181 gtcactgata caaacgatca ggatggaatt tggaaggcat tgaaagagtt aagcgtgatt
     3241 taaaagaggt tgtgaaaaag ctgttctgca tcaagtaata agcccagcca aacaaaaata
     3301 gctcctccta tttttcgttt ttctgggctt attaccgcag atgcaagctt tcgaacaccg
     3361 tttaaggaag aggtcgtgac agaagtggtc agtttcaaga aataagaagg gatttacgaa
     3421 aattgcttct caaatttttg taaaattacg gcttatttct gaagaagtag atgaaacaac
     3481 gtttattcgg gaaatgggac tgtgacaaga tgtttgtcat agtcccattt ttttatttta
     3541 tttttcaaaa ctaagaaata ccaggaaaga gttccttttg tttgaccttt tctttcttat
     3601 tgaaaacaac tataatatag agtgatgtta aaagagaaga ggaatttagt gtgcgggtat
     3661 tgtttattgg tgatgtagta ggttcaatgg gacgagagat gattactgaa tatcttccta
     3721 gattgaagaa gaagtaccgt ccgcaagtaa cgattgtaaa tggtgagaat gccgcttctg
     3781 gacgtggaat cacagaaaaa atctataaaa aatttttgca agacggcgta gatgtcgtga
     3841 cgatggggaa tcatacgtgg gacaaccgcg gaatctttga gtttgtaaat gaagctaaaa
     3901 agatggttcg tccggcaaat tttccggaag ggacgcctgg acaagggatg gtctttgtca
     3961 aagtgaacca agtcgaactg gcagtgatca acatgcaggc tcgttctttt atggtggact
     4021 tggatgatcc ttttcgtaaa atgaatgagc tggtagaaga agccagaaaa cgtacgccga
     4081 tcatttttgt agattttcat ggagaaacaa caagtgaaaa acaagcaatg ggctggtttt
     4141 tagatgggaa agtttcagca gtggtcggta cacatacaca cgttcagaca aatgatgcgc
     4201 gaattttgcc aagaggaaca gcgtatctga cagacgttgg gatgacaggt ccctatgatg
     4261 gtattttggg tatgcgcaga gaacctgtaa tcgaaaagtt tctgactgca ttaccaaaac
     4321 gttttgaagt tgtggaacaa ggccgatcga ttttaagcgg ctgtatcttg gaattggatg
     4381 atacgacagg tcatgcaaaa gaaatccagc tgattcagat caacgatgat cgaccgttca
     4441 tggagtagaa agagaggcag ttttttgcag caggagccgg aaattcaaaa agaagtaagg
     4501 aaactgacaa aacttttaag agagaatgaa acgatcattc gctacaaaga acttgaagaa
     4561 aagatccagc aaaaccagta tttagctgag ttgagagaaa aaatcaaaca agcgcaaaaa
     4621 gatgctgttc attttgccca ctatgacaaa ccagcagcag aaaaagaagc aatcaaacaa
     4681 gcggatcaat tcatgcaaga atttgatcag catccgcttg ttgtagctta tagaaaacag
     4741 ctgttggaag cagatgatct gcttcatcat ttaacaacta tgatacaaga agaaatcaat
     4801 ggacaaatag aggaggaaaa acatgcctca aaaaactaaa aatactccta tgatggaaca
     4861 atatctaagc atcaaggccc aataccaaga tgcttttttg ttctatcgtt taggagactt
     4921 ttatgaacta ttttacgaag atgcgatcaa agcgtcacag cttttggaat taacgttgac
     4981 gagcagaaat cgaaatgcag aagaacctat tcctatgtgc ggtgtacctc accatgcagc
     5041 acaaggatat atcgatgcct tgattgaaaa aggctataaa gtagcgattt gcgagcaggt
     5101 agaagatcca aagacgacta aagggatggt caagcgcgaa gtgatccagc tagtcactcc
     5161 tggtacagtc atggacagca aaggattgtc ggcaaaagac aacaattttt taacagctgt
     5221 agttagtcaa ggaaatgaat ttggctttgc ttatgccgat ttaagtacgg gagaattgaa
     5281 gacggctcgg ttgcatgatg aagaagctgt tttaaatgaa gcctctgcat tgcagacaaa
     5341 agaactcgta ttaggcagtg agatcactga tacccttcgt gaacagctaa gcgggcgttt
     5401 agggctagtc ttttctcaac aaaatgagat ggaagaaaac gcggaattta gctttttgac
     5461 aagcgaacta gtcgatccgc tggaaaaaga agcaaccggc aaactattga cttatttatc
     5521 ggtgacacaa aagcggagtt tagctcatat ccaaaaagca gtagaatacc aaccagatca
     5581 ttttttgaaa atggattatt attcaaaatt caacctagaa ttaagccaat ctattcggac
     5641 tgggaaaaag cacggcactc ttctatggtt gttagatgaa acaaaaacag caatgggtgg
     5701 ccgtttacta aaacaatggc tagaccgtcc gcttatccag gcaaaacaaa ttaaagcaag
     5761 acaagaaatg gtcgcttctt tgctagacag ctattttgaa cgtatcgatt tgcagtcagc
     5821 attaacgaaa gtctatgatc tggaacgcct agcaggtcga gtagctttcg gtaatgtcaa
     5881 tgggcgagac ttgatccagc tgaaaacttc tttagaacaa gtgccaatga tcagagggtt
     5941 gattgaagga atcgatcaag gtcaatggca aagtctgctt tcagaaatgc agccgatgga
     6001 ccatctcgtc caattgatcg accaagctat ccaagatgat ccgccgttac aaataacgga
     6061 aggaaacatc atcaaagatg gattcaatga gcagctcgat atgtaccgtt ctgctatgag
     6121 aaacggaaag aaatggttgg cagaacttga agcaaaagag cgtcaagaaa caggaatcaa
     6181 gaacttaaaa atcggattta accgcgtttt cggttattat atagaaatta caaaagcaaa
     6241 tcttggaaat gcagaactgg agaagtatga acgaaaacaa actttagcaa atgcagagcg
     6301 tttcatcaca ccagaactaa aagaattgga gacacaaatc ctcgaagcag aagaaaaatc
     6361 tgttgattta gaatatcagt tgtttttagc tgtgagagaa gaagtcaaaa aggctatcca
     6421 gccgctacaa gtacttgcaa aagcaatcag tgcagtagat gtgctacaaa gttttgcaac
     6481 gatcagcgaa cgctatcagt atgttcggcc agaacttgtc agtgacaagc atcagcttct
     6541 gatcaaagac ggacgacatc ctgtagtaga aaaagtatta ggacatcagg aatatatccc
     6601 taacagcgta gaaatggcag aagacgaaat gatcttgctg attacaggac cgaatatgtc
     6661 tggtaaaagt acgtatatgc gacagttggc attaactgtt ctgatggcgc aaatgggctg
     6721 tttcgttccg gcaaaacaag cagttttacc tatttttgat cggattttta cccgaattgg
     6781 tgcaagtgac gacttaattg ctggacaaag tacttttatg gtagagatga tggaagccaa
     6841 tcaagccctg cgttatgcta cgccaaacag tttgattttg tttgacgaac tagggcgagg
     6901 aacagcaacc tatgacggaa tggcgttggc ccaagcaatc atcgaatata tccatcgtca
     6961 tgtacaagca aaaacactct tttctactca ttatcatgaa ctgacggtac ttgagaaaga
     7021 acttccgcag cttaaaaatg tccatgttgg agccgttgaa aaagacggag aagttgtctt
     7081 cttgcataaa atgatggatg gacctgcgga taaaagctac ggcatacatg tggcaaagat
     7141 cgcaggattg ccttccaatc tactagaacg agcagcagat atcctgcata cgctagaaaa
     7201 tggcgataac ggacaccata ctgttccagc tgaagtagaa gagaaaacta aaacagccaa
     7261 acaggagcaa ataaaaccac ctgcagaaaa agtaaatcaa gaccatatac aagaccgtgt
     7321 aaaagaagaa aaccaacagc tttctttatt cagtgaatta tccgaaaatg aaacagaagt
     7381 cattgaatca ctgaagcaaa taaatttact agaaatgacg ccaatggatg cactaaacac
     7441 attatatgaa ttacaaaaac aactgtagga agaaggcgga tatcgatggc caaaatccaa
     7501 gaattatctg aacgtttagc taatcagatc gctgcgggag aagtagtgga gcgaccagct
     7561 tccgttgtga aagaattagt ggaaaatgca attgacgccg gcagtactca gattgatatc
     7621 ttagttgaag aagcgggact gcggacgatc caagtgattg ataacggaga aggcattttg
     7681 gcagacgacg tagagaatgc cttcaagcgg catgcgacaa gtaagatcca taatcgcgac
     7741 gatctttttc gcatacgaac gctgggcttt cgaggggaag ctttgccaag tattgcctct
     7801 gtttctgaaa tgactctgga aacggcgaat caggaagaaa aacaaggcac ctatctgtcg
     7861 ctgaaaggcg gagaagttat tgagcatcga ccagcaccac tgcgtcaagg aacgaagatt
     7921 accgtcagca atctgttttt caatacgcca gcccgcttga aatacgtgaa aacactgcac
     7981 acagaattag cgaacatcgg ggacatcgtc aatcgactgg cattgagtca tccatcgatt
     8041 gcttttcgtt tagtacatga tggaaacaaa atgctggcaa ctgctggaaa tggcgatctg
     8101 aaacaaacaa ttgcgggaat ctacggatta tctaccgcaa aaaaaatgct gaaaatcgaa
     8161 ggaaaagatc tagattttga gttatttggc tatgtttcat tgcctgaggt gactcgtgcc
     8221 agtaggaatt acttatcgac gatcatcaat ggacgtttta tcaagaactt ttccttgaat
     8281 aaagcaatcg ttgcaggtta tgggtcaaaa ttaatggtcg gtcgtttccc tattgctgta
     8341 ttggaaatca aaatggaccc gcttttagta gatgtaaatg ttcatccgac gaagcaggaa
     8401 gtccggctgt cgaaggaaaa agaactaaca gagctaatta gtaacgccat tcaaaaagca
     8461 ttgtctcaag aaaatctgat tccaagtgca gcggataatc tacggtttaa gaaaaaagta
     8521 cctgatcagc ctaaaacaga acaaatcgaa atcgatttgt cctacgcttt tgaagaagaa
     8581 aaacctaaaa agccaagtag tttgaatttt gatcgacaga caggtacctt tttcgtagaa
     8641 gaactaaaga acacatttgt tgacagaatt gttgataagt ctgtggaaaa tgttgaaaac
     8701 tattctggtt tttctgaaga aaacaccact tatccagaaa aaacagcgaa aacggtggat
     8761 aagtttggtg aaaatgttga taactttgta gaaaaaacag tggaaaaatc tgaaaagaaa
     8821 gaatttccca ttgtggataa catggagaaa acaaatgaag aacaagcaga taaatccgta
     8881 gaagaggatg aaaagattta cgaggaagaa ttgacactcc atcctgaatt tgatttcact
     8941 caaggaaacg acaaaacatt agaacaagcc atcaagaaat tagaaacgga aaaaccagta
     9001 caacgttttc ctcagttgga atattttgga cagatgcacg gaacgtatct ttttgcccaa
     9061 agtagcgatg ggttgtacat cattgatcag catgctgccc aagagcgcat taagtatgaa
     9121 tatttccggg aaaaaattgg ggaagtatct gatgatctgc aagaattgct tgttccattt
     9181 gtgttagact atccaaacag tgatgctttg aaattaaaag agcaaaaaca cttgctagaa
     9241 gatgcaggga tttatttaga agaatttggt caaaacagct ttattgtacg ggcccatcct
     9301 acttggtatc ccaatggaga agaagaatcg attattcggg agatgatcga tatgctgctg
     9361 acaactggaa gtgtgagtgt aaaaaaattc cgtgaagcaa cagcaatcat gatgagttgc
     9421 aaacgatcga ttaaagccaa ccattatttg aatgaagcac aagcacgtgt gctgctgaag
     9481 gatctggcac gatgcgaaaa tcctttcaat tgtccccatg gccgtccagt attgattcac
     9541 tttaccaatt cggatatgga aaggatgttc aaacgaatcc aagacccgca ttaagtcaaa
     9601 gagaagaggg aaaacatgaa gattgtatta gcatcccaat caccaagaag aaaagagtta
     9661 ttggctcata tcgtttcaga attcgagatt gaacctgctg atatcgatga aacgccattt
     9721 ccagacgaac agcccgtcga ttatgttcgt agaatggctg aagaaaaagc acgaactgtc
     9781 tgggaacaag atgaaaaaga agaccattta gtgattgcca gtgacacgac tgttgttctc
     9841 aatcaagaga tcatgggtaa accagaagat ctggctgagg ctgaattcat gctgaaaaaa
     9901 ctaagcgggg aaacacatac tgtatataca gctgttgttt taaaaacaaa ggaacgagaa
     9961 gaacggatac tagcagaagc acatgtcact ttttatcctt tgacagacga agagatcaaa
    10021 cgctacttgg agacggggga ttatgcggat aaggcagggg catacggcat ccaaaatgct
    10081 gcgagtgtat tcgtcaaaga aatttctgga gattattaca gtatcgtagg atttccgatt
    10141 ggtgctgtca atcaggcact gaaaacattc atttaaacaa aaaagcggac tatctgattt
    10201 tcgattgaaa atccatgata gcccgttttt tttattgaaa agattactta tctactctga
    10261 ctaagatttt acttcagctt taccgactgc ttcgcccggg cgaacaagtc ctgattggtc
    10321 aagagaaagt cctgcaactt tttcattatt ggtcaaagca acaataatgt cagttttctt
    10381 gccagcttgt tctatttctg gcaaatccat ttcagcaatc agggtctctg gtgtgacaag
    10441 gtatccttct ttcacgaata cattgaaagg aacacccttt agttcaacag tatctaatcc
    10501 catgtgaaca agtacttcca gcccctcatt agaaagaata ccaatggcat gttttgtttc
    10561 aaagacactc gtcactttac cgtttactgg tgcatagact ttgccgtttg acggttcgac
    10621 agcataaccg tcacccatca ttttttgtga gaaaactggg tcatttactt cagttatttc
    10681 cttgatggtc cctgcagcga ctgcgaataa tgtttctttt gttgcaactg ccgtaccagt
    10741 tgctgaaaca ggttcgaaag actgtggcgt tgctgttgcg ccatttgctg tttgaagttg
    10801 tacatcagca gcattttcag gaacagcagg atcaatctta ttcaaaattc cctgacgtct
    10861 aaagatgatc gtcaagccaa atggaatgat gacagcaatg atcattgcga tgatgaaagg
    10921 tacccatgtt tctgcttgga tagctagtat tcctggcaaa ccgcctactc cgatcgcgtt
    10981 agctcgaaca cccatcaagt tagcaaacat gccagctaat ccagagccga ccattgcagc
    11041 gacgaaagga tagacgtatt tcaaattgat tccgaacata gcaggttcag ttacgcctaa
    11101 gtaacaagaa atcattgctg gaatcgagat ttgttcttct tttttattcc cacgatgtaa
    11161 gaagatgatg gctaagactg cagagccttg ggcgatattt gataaagcaa tcatcggcca
    11221 taaattcgtt gatccgaaat ctgcaatcag ctgcatatcg atggcattgg tcatatggtg
    11281 gagaccagta atcactaaag gagcatacag gaatccgaat acggcactaa acaaccagct
    11341 gatagaagac gtcaatccag tatttacgat atttgagatc cagcttccga ctgtccagcc
    11401 gataggacct aaaataacgt gtgcagctaa aacagttggt aacaatgaaa acaatggaac
    11461 aaaaatcatc gagatcgact gagggatata tttgcggaag aagatttcta gatacgctaa
    11521 cataaaccct gccagcatgg ctggaataac ttgcgcttga taaccaatca tctggacttg
    11581 agcaaaacca aaatcccaaa atggaatatc tgctgcggct gtggaagcta cactataagc
    11641 atttaataat tgcggagaaa ctaacgtaat tcctaaaacg attcctaata tttgtgtagt
    11701 acccatcttt tttgcaatac tccaagtgat acctactgga agaaaatgga aaattgcttc
    11761 accgggtaac cacaaaaagg cattgacacc attccaaaac ttcgagtgtt caacgattgt
    11821 tccaccaaga ctttcaaact ggataccttc aagtacattt ctgaatccaa ggatcaatcc
    11881 accgacaatg attgcaggaa tcagtggtgt aaagatttct gctaaaacgg caatagcacg
    11941 ttgaacggga tgaaggtttt gttttgcagc tgcttttcct tgttctttgg aaaccccttc
    12001 aactccagat actgcaacga agtcgttata aaaagtagaa acatcgtttc ctatgatgac
    12061 ttggaactgt cctgcattcg tgaacatccc ttttacgcta ggtatatctt cgatcgcttt
    12121 ttcgtccgct tttcctggat cattcaggac aaaacgcata cgagtcgcac aatgactaac
    12181 tgcagcaata ttttcttttc cgccgatatc atgtaacagc ttctcggcat ctgcttgata
    12241 ttttgccata tcaataaacc cctttctctt aacttgtcta tgcaagttgt ttcttcattt
    12301 actatacttt ttttcggaga gaattgcaag cgttttatta aaaagtacga gagaaaatga
    12361 tatcgtttta aaaagcttaa aataagcttt tcttaaaaat aggaacattg aaatacaaaa
    12421 agagctgtag tatagtgaag aaatgatgga agaacgagtg agggaaaaaa catgaataag
    12481 ttcaatgaaa tttttttaga tttggaacag aaaattttag cgaaagaata tcctccgcat
    12541 tcgcttttac ccagtgagaa ccaattgatc aaaatttatg gtgtttcacg agaaacaatt
    12601 agaaaagcgc tgaatctttt gactacggaa ggttatattc aaaaaaaaca aggaaaaggt
    12661 tcgatcgtac tggatcggaa tcgcttcgac tttcctattt ctggattgac tagctataaa
    12721 gagctacagg agacccaacg tattccaagt gaaacgatca tccatacgtt agaagaaaca
    12781 gaagtgacaa aagcattgaa cgagatcact ggctgggaga tcggtgcgcc tgtttggcat
    12841 ttgatacgcg aaagaaaaat caatggcgaa gtagttatat tagacaccga ttatctatta
    12901 aaggaaatcg ttcctatttt aactccagca caagcacaag gatcgatcta tgaatatttt
    12961 gaaaatgagc tttctttgac gattgcttac gcgcaaaagg agatcacagt agaagaagta
    13021 acggaaatca tgaaaacagc gatggatctt cacgatgaca cttatgcagt agtagtcaga
    13081 agcctcgttc atctggaaga tacccgctgt ttcgagtatt ctgagtcggt tcaccgatta
    13141 gataaattcc gtttcgttga gtttgcccga cgccgaaaag tgtaaatagt tcaaaagaaa
    13201 gcaaattgtt ggcatctgtc cactttggcg ctatgatagg atcacaaagg gggcattttt
    13261 aatggataaa ccaataagac cagaaaaaga agaactgaaa gaaaagttat cacccatggc
    13321 ttatgcagtc acacaagaaa atgcaacaga acgaccattt agcgggaaat atgatgattt
    13381 ttacgaaaag gggatctatg tagatatcgt cagcggtgaa ccgttattca gctctgcaga
    13441 aaaatatgat gcaggttgtg gctggccatc attcagcaaa ccaataacga aaagacaagt
    13501 gcgcgaaaaa gccgattttt ctcatggcat gcatcgaata gaagttcgaa gcaaagaagc
    13561 agattctcat ctcggacatg tgtttaccga tggaccagta gatcaaggtg gtctacgtta
    13621 ctgcatcaat tctgcctcac tgaaattcat tccatatgaa aagatggatg aattaggtta
    13681 tggggaattc aaatcacttg tagaataatg gaaatacaca aagagcggta caatacgggg
    13741 atttactcga tcgtccgctc tttttttatt caagcgattg ctatttgaaa gaaacgctcc
    13801 ctagactcct ttttttcaca aggtgaaatg tgctacaata aagggaacgt atgtgtgcaa
    13861 aagaaagagg actagaatat gtatgaatat ctgacaggtg tggttacctt tatcaacccc
    13921 tattacatag tagtagaaac caatggtatc ggctaccaga tcgcactagg aaatccttac
    13981 cgctacagta gcaaactaaa tcaaaaaata aaaatttatg tccatcaggt aatacgtgaa
    14041 gatgcgcatc ttctgtatgg atttgattcg ctagaagaaa agcaactgtt tttgcgatta
    14101 gtaagtgttt caggaatcgg tccgaaaagt gcccttgcaa tcatggcaag cgaagaccac
    14161 tccggtttga ttcaagcagt agaatccagt gatgtcactt atttgacgaa atttccagga
    14221 gtagggaaaa agacagcaca acaaatgatc ttggatctaa aaggaaaatt cggtgagctg
    14281 agtatcgata caccttttag tctatttgat gaagcaaaca caaaagacgc tacagctcta
    14341 tctgaagcaa tggaagctct ctcggctctt ggttacagtg atcgagaaat caaacgtgta
    14401 gaaaaacaat taaaagaagt agataatcag acaacggatg agtatctacg ccaagcactc
    14461 aagctaatga tgaaaaaata aggaggagac caaatgacag atgaatactt gttatcacca
    14521 gaatctggcg aaaatgaact gagtttggaa aaatctcttc gaccacagct gctttctcaa
    14581 tatatcggac agagaaaagt caaacaagaa cttcagattt acatcgaagc tgccagaaat
    14641 cgggaagaac ctttagatca tgttctgttg tacgggcctc ctggattagg aaaaacgacg
    14701 atggccatgg tcattgctaa tgagatgacc gtaaatattc gtacgacgag tggccctgct
    14761 atcgaacgtc cgggtgactt agtagcaatc ttgaatgaac tggaagcagg ggatgtcctg
    14821 tttatcgatg agatccatcg attacctaga gtggtagaag agatgcttta ttcagctatg
    14881 gaagatttct atgtagatat catggtagga caagggccaa ccgcacatcc ggttcatttc
    14941 cctcttcctc cctttacttt gattggcgcg acgacacggg caggtatgct ttctgcacct
    15001 ttacgagatc gatttggcat catttctcat atggaatatt atgacgaaca ggatctgcag
    15061 gagatcgttt tacgttcttc ggatattttt caaacagaga tcattgaaga aggggcattg
    15121 gaaattgctc gccgatcacg aggaacgcct agaatcgcta atcgcttgct caaacgggtt
    15181 cgtgattttg cgcaagtcca aggagacggg aaaatcgatc gtcctatagc ggatcaggca
    15241 cttactttgc tacaggtcga tcaggctgga ttggattatg ttgaccagaa attgttgcga
    15301 acaatgatcg agttatatgg cggcggacca gtagggttaa gtacgctttc agtaaacata
    15361 ggagaagagc gtgagacggt agaagatatg tatgaaccgt atctgatcca aaaaggattt
    15421 ttaaagcgta cacctagagg gcgaattgct actgcttatg cctatgaaca ttttggttac
    15481 gactatcata tatgattttt gcttttagat tggaggaaaa tgaatgagtg atacaacacg
    15541 tacgaaacaa ttgatcaaaa acgtattgat tgatttgtgc caaacgcaat cttttcgaaa
    15601 aatcagcgtc caaaaaatcg caaacgaagc aaaaatcaat cgacaaacat tttattatca
    15661 ttttacagat aaatacgatt tgcttcgttg ggtctattgt gaggatgcat tgcattattt
    15721 agatccacaa tgtctttcac ttgataattg ggaagagcaa gcattgaaaa tgctgaaagc
    15781 aattgctgag aagaaagaat tttatgcaaa tactgtgctt tctgacccag aaattttaca
    15841 aaaagagttt tctcaactga tccaaaaatc atttatcaag atttttgaac aaatggatga
    15901 agaaagacaa ctttctgcaa gagacaaact attttatgct cgtttttttt cttacggttg
    15961 cagtggtgta ttagtcaatt ggatcacgaa tgaatatgaa gaaacaccga tggaaatcgc
    16021 tatgcagctt ttccgtttag ccaaggatac ggaattatat tcttatcgtc tttatgcaaa
    16081 agaagagttg gaagaacagt aatagaaaaa ttttttgttg gaaaagcaat aatcgctaaa
    16141 aggagatggg gaaatgacaa aaattttatt tgtttgtttg ggaaatattt gccgttcacc
    16201 gatggcagaa ggcttgctga gaaaacgaat agcattagaa ggaagggaag cagaattttt
    16261 tgtggattct gctgctacaa gcacgtatga agttggaaag acaccgcacc ctggaacgaa
    16321 aaaaatatta aaccaagaaa aagtagatat gacgaatatg gttgctcggc aaatcacacc
    16381 ccacgatttt gaaacttttg attggatcat tggcatggat caagaaaatg tagaagaact
    16441 gaaacgaaga gcaccacgct cagcgcaggg gaaaatccat ttgtttttaa gtagcattcc
    16501 tggaaaagaa aaagaaaatg ttcccgaccc atactatacc aataactttg atgagacata
    16561 tcacttgatc acagaaggcc tgaatcattg gtggaatatt tggattgatg aaaataaaat
    16621 aacaaacaat ttgtaaagtt aacgctttct ttttaaggcg atgttagagc atttccttgt
    16681 ttttttgtaa aatgttaggt aaaataaaat taattaagta acactttctt agctaaagga
    16741 ggagtgcatt cgatgaacga agagacagta tcgccaatga agaagcttac gaagttagtt
    16801 actcatactt cgtatcaact ttttgattct tttgtaaatg acactgaaga acatgctaag
    16861 aatgtacgat tgcaaggaaa gtttattctt gagctgcaaa aagctgctac tgaacatagt
    16921 ctagttatct tacaagtaag agcaaatcgt ttgagtagca agtatgaaac gatctcaggc
    16981 tgggtggcga caaaaacggt cgaaaaagac cgagtgatga tccgcctaca aaacgaagag
    17041 cagcagatcc ggatggttcc tgtagaacaa attgaaaaaa taacaatcct ttcaccagca
    17101 ggggaacagg aacggttatc aaaataattt ataccagata gaaaagcacg tatgagcttg
    17161 taccatcaat cattactaat caaaaataac cgaatgcttt aagctgatat cagtaaaacg
    17221 gaacgtttta ggatgagtat catagcatga cagcattcgg ttgattagtt gaatcgattg
    17281 gatggtacaa gtttttttga taaaaaaagg tcctgctagc cggggagcta gcaggataag
    17341 gagttaaaaa tgaaaaagtg ttgttagggt tgtttgttgg tatgcttata taatagtcat
    17401 tagttgtgaa tattttgtga cgaaagtttt cgaaagttgt taagtttttg agagt
//
LOCUS       DAYYKN010000054        17379 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000054 DAYYKN010000000
VERSION     DAYYKN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.054"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACHBB7_001511"
     CDS             complement(24..722)
                     /locus_tag="ACHBB7_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM9209470.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACHBB7_001512"
     CDS             complement(715..2067)
                     /locus_tag="ACHBB7_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM9209471.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACHBB7_001513"
     CDS             complement(2290..3690)
                     /locus_tag="ACHBB7_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9209472.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACHBB7_001514"
     CDS             3851..4588
                     /locus_tag="ACHBB7_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM9209473.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACHBB7_001515"
     CDS             4713..5192
                     /locus_tag="ACHBB7_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM9209474.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACHBB7_001516"
     CDS             5189..5956
                     /locus_tag="ACHBB7_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM9209475.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACHBB7_001517"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACHBB7_001517"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9209476.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACHBB7_001518"
     CDS             complement(6859..7461)
                     /locus_tag="ACHBB7_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM9209477.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACHBB7_001519"
     CDS             7567..7797
                     /locus_tag="ACHBB7_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209478.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACHBB7_001520"
     CDS             7788..8036
                     /locus_tag="ACHBB7_001520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9209479.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACHBB7_001521"
     CDS             8142..8726
                     /locus_tag="ACHBB7_001521"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM9209480.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACHBB7_001522"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACHBB7_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM9209481.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACHBB7_001523"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACHBB7_001523"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM9209482.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACHBB7_001524"
     CDS             10627..10806
                     /locus_tag="ACHBB7_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209483.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACHBB7_001525"
     CDS             10828..11853
                     /locus_tag="ACHBB7_001525"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM9209484.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12044..13288
                     /gene="glyA"
                     /locus_tag="ACHBB7_001526"
     CDS             12044..13288
                     /gene="glyA"
                     /locus_tag="ACHBB7_001526"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM9209485.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13441..14388
                     /locus_tag="ACHBB7_001527"
     CDS             13441..14388
                     /locus_tag="ACHBB7_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM9209486.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14522..15151
                     /gene="upp"
                     /locus_tag="ACHBB7_001528"
     CDS             14522..15151
                     /gene="upp"
                     /locus_tag="ACHBB7_001528"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM9209487.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15290..15532)
                     /locus_tag="ACHBB7_001529"
     CDS             complement(15290..15532)
                     /locus_tag="ACHBB7_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209488.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15653..15725
                     /locus_tag="ACHBB7_001530"
     tRNA            15653..15725
                     /locus_tag="ACHBB7_001530"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15686..15688,aa:Thr,seq:cgt)
     gene            complement(15844..16482)
                     /locus_tag="ACHBB7_001531"
     CDS             complement(15844..16482)
                     /locus_tag="ACHBB7_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM9209489.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16605..17237)
                     /locus_tag="ACHBB7_001532"
     CDS             complement(16605..17237)
                     /locus_tag="ACHBB7_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM9209490.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT         5929 a         3080 c         3446 g         4924 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaattgtagg aaaataaaaa ggagctgttg tttgtggtag attacaaaac
    12061 gtttgaccct gatctttggg ctgcgatcgc caaagaagaa gaaagacaag aacataattt
    12121 ggagctgatt gcctctgaaa actttgtctc cgaagcagtg atggctgcac aaggaagtat
    12181 cttgacaaat aaatacgcag aaggttatcc agggcatcgc tattatggtg ggtgtgaatt
    12241 tgtcgatatc gttgaaaatt tagcgattga ccgtgcaaaa gaattattcg gagcgaaatt
    12301 tgcgaacgtc cagcctcact ccggctctca agccaacacg gctgcttatc tcgctttggt
    12361 agaaccaggg gacacgatct taggaatgga tctttccgca ggcggacatc taacacatgg
    12421 atcaccagtc aacttcagtg gcaaaaccta tcactttgtt gcttacggtg ttgatccgac
    12481 aacagaagtt attgattata atgtggtacg tattttagct agaaaacatc aaccaaaatt
    12541 gatcgtagca ggtgccagtg cttacggacg aacgatcgac tttgcgaaat tcagagaaat
    12601 cgcagatgaa gtaggcgcaa aactgatggt agatatggca catatcgcag gattagtagc
    12661 agcaggactt catcctaatc cagttcctta tgcagatatc acaacgacta ccactcataa
    12721 aacattgcgt ggccctcgag gcggcatgat tttgacaaac gatgaagcat tagcaaaaaa
    12781 aataaatagt gcggtatttc ctggtattca aggcggccct ctagagcatg tgattgcggg
    12841 taaagcagta gcattcaaag aagcattaga tcctgcattt aaagaatata gtgaacaaat
    12901 catcgccaat gcaaaagcaa tggtcaaagt tttcaatcaa gcaattggaa ccagagtcat
    12961 ttcaggggcg acagataatc atctgatgtt gatcgatgtt cgcgagttag gtataaatgg
    13021 aaaagaagca gagagcattt tggacagtgt gaatatcact gtaaataaaa actcgattcc
    13081 ttttgaaaca ctaagtccat tcaaaacaag cggtattcga atcggcacgc cagcaatcac
    13141 tactcgcggg ttcaaagaag aagatgctgt aaaagtggct gaactagttg tcaaagcatt
    13201 acaagcaaaa gatgataacg ctcaactaga cgaagtcaaa acaggtgtac gcgaattaac
    13261 tgaaaaattt ccgttgcata aaaaataaaa atttaaattc cattagtaaa cagaaaagtt
    13321 caatgttggg cttttctgtt tattttttgg gaaaacggat tctttgacaa tatcggtgat
    13381 gctctctagt atatgaggtg taattttagt cttcacacta aatctcaggg aggaaaagtc
    13441 atgactgatc gtatctcgtt agaaaaggaa gcagtagcat tcagtgatgc aaatgcgcct
    13501 catccgcgta tctatgaact agcaccggaa gaaggaagag ttttactaga agaagtacaa
    13561 gaatcacctg tgaacaaata ttccgtcgat attgaagact tgatggtgga tactggcgaa
    13621 tggggaaaac tcaatattcg tctcgtacga ccagaaggga atatggataa attaccagtt
    13681 attttttata ttcatggagc aggctgggtt tttggcagtg ctcaaaccca tgataaattg
    13741 atcagagaac ttgcggttcg tacgaattcc gtcgttgtct ttccagaata tagtcgatca
    13801 ccagaagcaa aatatccaac agcaattgaa caaaattatg ctgtgttgca acagctgaat
    13861 aaattagcca agtcaaaaaa tctggatctc gagaatctaa tcgttgcagg agattcagta
    13921 ggtggtaaca tggctacagt catgacgatc ttaacaaaag aacgaaaggg cctcccaata
    13981 tccaagcaat tgctgtatta tccagtcaca aacgctgatt ttgatacaga ttcctatcat
    14041 gaattcggag aaaactattt tttgacaaga gaaggaatga tgtggttttg ggaccaatat
    14101 acgacgaatg aagaagagcg tgctgaaatc acagcttcgc ctttgagagc aaatataaat
    14161 gaactaaatg atcttccttc agcaatgatt ttaacgggag aagcggatgt attaagagat
    14221 gaaggtgagg cttatgcgcg caaacttcgt gatgcagggg ttcctgttac acaagtacgc
    14281 ttccaaggaa tcatccatga ttttgtcatg gtaaatgcta tggatcagac aaaggcaacg
    14341 cgtgcagcaa tggcattatc tacttcttgg atcaagaaaa aagaataagc tttgcttgtc
    14401 cgcaggaaaa cttcttttca aaaacggatt ttcttaggaa aaatggcgag tttgactagt
    14461 caatcacttg attttgtttt acaattaaaa agaaacgaca gaacaaagga gagcttaaaa
    14521 aatgggcaaa tttcaagtga tcgatcatcc attgattcaa cacaaactaa ctattattcg
    14581 ggacaaaaac tgcggaacaa aggtttttcg tgaagtagtc gatgaaatcg ctatgttgat
    14641 ggcatatgaa gtatcacggg atatgccatt ggaagacatc gtgatcgaaa caccgatcac
    14701 tgaaacaaca caaaaaacgt tgtctggtaa aaaagtagcg attattccaa tcttgcgcgc
    14761 aggtattgga atggttgacg gtatcttgga attgattcca gctgctaaag tgggacatat
    14821 cggtctttat cgagatgaag aaacattgga accacatgaa tatttcgtca aacttccaga
    14881 agacattgat gcacgtcagt tgtttgtagt agatccaatg ttggcaacag gcggatcagc
    14941 aatcatggca atcgatgcat tgaaagaacg tggcgcgtcg aacattaaat ttgtttgttt
    15001 ggtagcagca cctgagggag taaaagcttt gcaagaagcg catcctgata ttgacatcta
    15061 tacagcttct ctagatgaaa aattgaatga acatggttat atcgttcctg gactagggga
    15121 tgcgggtgac cgtttgtttg gtacaaaata agtaaatacg aaaaagggtg tgacacaact
    15181 tttgtcacat ccttttttgg tcatttttaa cgtttgatgc agaacagctt tttcacagct
    15241 ttcgtttgca tataaaaaaa ccatcccaaa aatgcagaga tggtcaaatt caaatctttt
    15301 ttagttcttt gtagttaacg attactacac ccttactttg cgtcatcaag ttcgcatttt
    15361 ctgcagtttc atcaatctca acaacagcgg aatttttcaa ttgctttgaa atcgttccag
    15421 tttgcgactt gccgtgaatt gtgaacatta cgtgagtatt gatcttgaat ttttcaccaa
    15481 tttccgatgg tacgatagtt gtatcaaatt ttccaaaagt tgccattttc ataaaacctg
    15541 cctttcacat caattatacc acgttttatg aaaaatatca tgaaattaaa ctaattttcc
    15601 atcggactct ttacaatcag ccaaatagag tatataattt cttttggata aggccagctt
    15661 agctcagttg gtagagcaac gcactcgtaa cgcgtaggtc gtaggttcga tccctatagc
    15721 tggcattaaa agagaagaca gatatatcaa ggatttttaa tccattgata catctgtctt
    15781 tttgtttaaa ggaaataaag tatgctttct tagcaataga gcatgtatta tttctataaa
    15841 aatttaggag aataaaacga ctagcaatat agctgtgaaa tcaaatatca catgaactgc
    15901 ccaacttgta atgatcgagt tgcttttaat ggcagcttgg ttgaataaca atcttgccac
    15961 cccttgtatc aaaaggatgt gtgctacagt gtgtaaaata tttccattat cataagtact
    16021 gtaatggata aatccaaaga tgattgctga caagaaacta gcgacaggaa tgctgaactt
    16081 actagaaaaa atagcgagaa aatacactga gaacaattct tctccaagta tcatgatagg
    16141 caaaataata aataataacg ggctgaaagc ctcatttatt ggattgcctg ttaaacccca
    16201 tttcaaaatt tgttgcaaaa ataccgagac tgcggcactg attactacat tgataaaaaa
    16261 atatttggct atatttttga caggagcgac aggtttcgag aacatttttt tcgtatcttt
    16321 tttaccaaaa acaagtgtaa atgctataaa tccagtgata gttaaagaaa aaatgctaat
    16381 aatggatggc gcaaatagca accctaacaa accgagtaca acgacaatag tgcccaatat
    16441 ccctttttta agttctattt tttttcctaa aatgttattc atgataataa ctcctactat
    16501 tcagtttttg aagatagagt cagtatacat gaagagttaa aataggcaat gtttttttat
    16561 ggatatttgt attgctaaaa atttatctta tatattcatt ttgattatac tacttttatt
    16621 tgtaaaaatt ttctagcaaa accaagtgct tgcaactgat taagcgtaca attttttaga
    16681 tgttccgagc gtacttcaat catttcaaag gagttgctac ttaaatcgat attttttaaa
    16741 tcagtatcaa aaaagctagc attttctaaa tcattagtag aaaacattat tttagtgagg
    16801 gaacagtcta caaaatctgc gcttgataag acagagtgct caaaggtact ttcttttatg
    16861 tttgaaccag ataaatttac atagtccatt ttacaattct cgaataacgt gttcccaata
    16921 taactttcca tcaatttagt acctgttaat ttacaattga caaatttcac gcgatgcaac
    16981 atgctttttg tcaatataca gcctgaaaaa tcacagtttt cgaatataca gtcggtaatc
    17041 tccattcttt gaaaatcatt ggaagcaaaa aatgcatact tgaaaataga tttgtaaata
    17101 attggatggc agattatttg gttttcgatg acctcttttt gtgaaaggat attatacagt
    17161 tcaaaatctt cgttttcata gatatgaaaa aaggatcctt tattcaagct tgcttcatta
    17221 actttaggaa aaatcatatt taattacctc cattttcata aagtcatatt aacaaaagct
    17281 aaaccaactt acattagcag aaaatgagat aggttttcgc ctttttcgat aacgatagtg
    17341 tcgctatctc ttgaaacgtt cttagaattg ccctgagag
//
LOCUS       DAYYKN010000055        17099 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000055 DAYYKN010000000
VERSION     DAYYKN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.055"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..579)
                     /locus_tag="ACHBB7_001533"
     CDS             complement(58..579)
                     /locus_tag="ACHBB7_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM9209491.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            777..4457
                     /gene="nifJ"
                     /locus_tag="ACHBB7_001534"
     CDS             777..4457
                     /gene="nifJ"
                     /locus_tag="ACHBB7_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM9209492.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            4646..5533
                     /gene="cdaA"
                     /locus_tag="ACHBB7_001535"
     CDS             4646..5533
                     /gene="cdaA"
                     /locus_tag="ACHBB7_001535"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM9209493.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5530..6699
                     /locus_tag="ACHBB7_001536"
     CDS             5530..6699
                     /locus_tag="ACHBB7_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM9209494.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6723..8078
                     /gene="glmM"
                     /locus_tag="ACHBB7_001537"
     CDS             6723..8078
                     /gene="glmM"
                     /locus_tag="ACHBB7_001537"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM9209495.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8150..9955)
                     /gene="glmS"
                     /locus_tag="ACHBB7_001538"
     CDS             complement(8150..9955)
                     /gene="glmS"
                     /locus_tag="ACHBB7_001538"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM9209496.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10365..10655
                     /locus_tag="ACHBB7_001539"
     CDS             10365..10655
                     /locus_tag="ACHBB7_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209497.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10668..11693
                     /gene="sppA"
                     /locus_tag="ACHBB7_001540"
     CDS             10668..11693
                     /gene="sppA"
                     /locus_tag="ACHBB7_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM9209498.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11696..12355
                     /locus_tag="ACHBB7_001541"
     CDS             11696..12355
                     /locus_tag="ACHBB7_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM9209499.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12512..13444
                     /locus_tag="ACHBB7_001542"
     CDS             12512..13444
                     /locus_tag="ACHBB7_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209500.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13598..14887
                     /gene="tig"
                     /locus_tag="ACHBB7_001543"
     CDS             13598..14887
                     /gene="tig"
                     /locus_tag="ACHBB7_001543"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM9209501.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15134..16384
                     /gene="clpX"
                     /locus_tag="ACHBB7_001544"
     CDS             15134..16384
                     /gene="clpX"
                     /locus_tag="ACHBB7_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM9209502.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16467..17054
                     /gene="yihA"
                     /locus_tag="ACHBB7_001545"
     CDS             16467..17054
                     /gene="yihA"
                     /locus_tag="ACHBB7_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM9209503.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         5600 a         3050 c         3746 g         4703 t
ORIGIN      
        1 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ctacgctcct tattttttta
       61 ttttgtttgg atcaattctt ttaattcctc tattgaaata gattcatcaa agatttcttt
      121 acccaagatg acagtcggaa caaatcgaat atgtgcttgc tcagcttcgt tgatgacagc
      181 ttggctcagc tgttcgtctt tttgttctgt cagctttagt ttatctactg caaaatccgc
      241 tacttcttgg agagaaagat gtttccactc gtcttgagta tcgaatattt gtttgatctc
      301 tttgatcgct ttttgaccat cgctgattgt gagataccgg tgcatcacgt ttcctcgctg
      361 caagctttct ttttctttat cgaacaattt gatcactcgc tgcaattgcc cgctttggac
      421 tgcttcttct aatagctcat aagattcttc aaaccattgt cggcaatacg gacaagatag
      481 gttgatgaat tcgaccattt ttttaggagc attcgatgaa ccataaagga ttcctttttg
      541 tgtatttgtt ttcgttgcat caatcactga tatatccatg ttaaaacacc tctttcgctt
      601 atagtttata catgtctgac aaaaaaaggc aacgatttag actgtttgtc taaaaattag
      661 atgaccaatt caaatagaca tatgtgaaat atttttataa ataaaagaaa aaatggaatg
      721 ttttacgtta taatggaaca gataataaga gagaatatga acaaggatga tgaatcatgc
      781 gaaaaatgaa aacaatggat ggcaatacgg cagccgcata tatttcttat gcatttaccg
      841 agattgctgc aatatatcca ataacaccca gttcgaccat ggctgaatta gtagatcaat
      901 ggtcagcagc aggaaaagaa aatgtattcg gtcaaccagt aaaagtagta gaaatgcaat
      961 ccgaagcagg agcagctggt gtggtccatg gatcattgaa aactggtgca ctgacaacta
     1021 catataccgc ttctcaaggg ctgcttttga tgataccgaa tatgtacaag attgctgggg
     1081 aactactgcc aagtgttttc catgttgcta gtcgggcagt gacgacaaat gcgttgaata
     1141 ttttcggaga tcacggagac gtgatggctg caagacaaac aggatttgct atgttagctg
     1201 aaagcagtgt acaagaagtg atggatttag cacctgttgc ccatcttgct tctattgaag
     1261 gagaagtacc ttttttgaat ttctttgatg gattccgtac gagtcacgag attcaaaaaa
     1321 tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa tcaggagaaa ttagcttcat
     1381 ttcgtaaacg agggatgaat cccaatcatc cgactgttag tggtacgaac cagaatccag
     1441 acatccattt tcaacaacgg gaaacgatca acccctatta tgataaatta cctgaaatcg
     1501 ttcaaaaata tatgaaagag atcaaccgat tacgaggaac ttcttatgac ttggtgactt
     1561 attacggagc agacgatgcg gaagaagtca ttgtttctat gggatcagtt gcacagacga
     1621 ttgaacaaac gattgattac ttgaatgcaa acggacggaa aacaggtttt ttgaatatcc
     1681 atctttatcg tccttttcca gtggaaacat tcgtgcaaaa gttgcctcat acagtaaaaa
     1741 caattgctgt tcttgatcga agtaaagaac caggggcagg tggagaacct ctcttattag
     1801 acgttcaaag tgcgttatat gattctgaac ttcgcccggc tgttattggt ggtcgctacg
     1861 gattggggtc taaagatgtg actcccgacc aaatctcagc agtttttgat gaattgaaaa
     1921 aagacccaag catacgcaaa aaacggttta ccatagggat cgtggatgat gtgacttatc
     1981 aatctttgga accaaaggaa agtctggatt tgacagaacc gcaaacattc caggccaagt
     2041 tttggggatt tggatctgac ggaacagttg gagcaaataa atcagctatc aagattattg
     2101 gagatcacac cgataaatat gcgcaaggct atttttatta tgattcgaaa aaatctggcg
     2161 gtctgactgt ctctcatctt cgttttggag atacgccgat acgttcagcg tatcttgtcg
     2221 agcatgcgga ccttgttgcc tgtcacacac cagcttacct gcatagctat gacttggtca
     2281 aaggattgaa gccaggtggc atttttttat tgaatactct ttggagcgac gaacaattag
     2341 aaacgcatct gcccttaaaa ttaaaacgtt acttggctga aaataacatt agattctata
     2401 cgatcaatgc catgagattg gcccaagaag tcggtttggg acgccggatc aatacggcaa
     2461 tggaaacggc tttttttaaa ttagctgaca tcattccttt tgatgaggtg cttccgctgc
     2521 taaaagaaga agccctgaaa agctatggac ataaatcaat ggctatcgta gaaaaaaaca
     2581 tacaagcaat cgatcggacg accgaactgc ttcatcaagt cgcgatccca gaccattgga
     2641 gaacgttgga gatgcctgaa aaaatcaata aaaggaacac tggttatata caagaaatac
     2701 ttgaaccagt gaatgcacag gaaggaaatc aactctctgt tggtacactt gtagcaaatg
     2761 gaatgacaac tggcgaaatg cctttaggaa cggcttctat ggaaaagcgg ggcgtcgctt
     2821 tagaagtacc tgagtggatt agtgatcgct gtacgatgtg taatgaatgt gcctttgttt
     2881 gtcctcatgc tgccatccgt ccatttttag cagatgaaga ggaaatggaa gaagcgccag
     2941 aaggatttat cgtgagagaa atgcgaggcg cagacggcgt aaaataccgt atccaagttt
     3001 cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc ttgtcccgca aaagaaaaag
     3061 ctttagtgat gaagccttat gaagaacaga aagaacaagc tgtgaattgg gcttttgcaa
     3121 tgacattaag acaaaaagca aatccagtca aaggaaagaa tactgtcttg tctacacaat
     3181 tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg ctgtggcgaa acaccttatg
     3241 taaaattatt gacacaaatg ttcggtgatc gaatgatgat cgcgaatgcg acaggctgtt
     3301 cttctatttg gggcggtgct tctcctgctt ctccttatac gaccaatgaa tgtggtcaag
     3361 ggcctgcatg gtcaaattct ttattggaag ataatgctga atttggttat ggtatgcttc
     3421 tagcggcaca gactcgtcgc gaggctttgg caagttcaat caaagaagta ttgccaaaag
     3481 tatcagaaga actacgagaa ttgatggaag actggttggt acacatgttt gagagcgaag
     3541 gaacgcagca aagagcagca aaactgcaag ctgcgatcga aagcgaacga tcaattgtcc
     3601 ctgaactgga ggatatatac aagagaaagg atctattcgt aaaaaacagc caatggatga
     3661 ttggtggaga cggatgggct tacgacattg gttttggcgg gatcgatcat gtacttgcca
     3721 gcggagcaga cgtgaatatt cttgttttgg ataacgaagt ctattccaat actggcggtc
     3781 aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt ttcggctagt ggaaagtatg
     3841 cttcaaagaa agatctagga atgatggcga tgacatatgg aaatgtttat gtggcgcaaa
     3901 ttgcttcagg tgccaatcaa atgcagacga taaaggcttt tgaagaagca gaaaagtatc
     3961 ctggaccttc tttaatcatt gcctatacgc cttgtatcac acatggtttg cttggtggaa
     4021 tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc tggctattgg tcattatatc
     4081 gatataaccc tgcaatggtc gaaaaaaaca agaacccaat gactttggac tacaaaaaac
     4141 ctgatttctc tgctatgcct gatttcatga ggaaacaagt tcgcttttcc tcgctagaga
     4201 acgcaaatcc aaatcttgca aaagctttat ttgaaaagac tatccaagac gctaaaacac
     4261 gtttttacaa ctatgctgca ctgacgggag aattagaaaa aatcaagaaa cgacttgaac
     4321 cagaggaatc tatcgatgta cccgttaaga ctagaaaaga aaaaacgacg gaccttgaag
     4381 cagaagaaag aagagctgcc agagcagaac gacgtaaaaa aatggaacaa gctacaaaag
     4441 aaaaaaagga aaaataacag aaaaaatata atgaataatt ctctgaagtt taaaaatatt
     4501 tattgcagag aaaaataaaa cgcatacttg caattttcct gaaaattatg cgtttttctc
     4561 aagaaaaata cgagcagatc agcctctaaa attaaaacat atgatatact agagaggaaa
     4621 tctcaaaaga gaagagaggc tttttatgtc atttcaaatc ggggaacttt ttaatttgga
     4681 ctattggcag caaatgtttt ccggtgactt gttctctctg aattttttgg tgaacatact
     4741 tgatattctg gttgtctggt atcttgtata caaactgatc caactgctgc gaggcacaaa
     4801 agcgattcag cttctgaaag gtgtagctat ttttattgtt atccgaatcg tagcggaaat
     4861 cattggtctg cacaccttgt cttggctgat gaaccaagtc attacgtacg gggtgatagc
     4921 tgctatcgtg atttttcagc cggaaatccg gagaggactg gaacatcttg gacgcagttc
     4981 gcttttcaaa caatcaaaaa gcgaacagca ggaagatgag aagatgatct tgtcttttga
     5041 caaagccatc caatatatgt ccaagcgaaa aatcggtgcg ctaattacta tcgaacggca
     5101 tactggatta gatgagtata ttgagacagg aattgcatta gatgcagata tcactggaga
     5161 gctgctgatc aatatcttta ttcctaatac accgctgcat gacggtgcag ttattgtaaa
     5221 agaaggaaag atcgcagtag caagcgccta tcttcctcta tcagaaagta tgttgattcc
     5281 aaaagaattc gggactcgac accgggcagc tgtcggtata agcgaagtca gcgacgcgat
     5341 taccatcgtt gtctctgaag aaactgggga tgtcagcatt acattagata atgaactgat
     5401 ggctggctta tcccagcaag aatatttggc aattttgcgt agagaactga ttgctgaaga
     5461 gaaaacaaaa aacaagaaaa acgtgattca atctttccta gacggtatga ctaagggagg
     5521 ggatcgcaaa tgatctcaaa agagaaacgg acaagcattc tttacagttt tattgcattg
     5581 ctgttcagta ttgcgctgta cttcaatgca aatggccagt ccgttccaaa tacgctttct
     5641 ggaaatgaag cgtataatca aacagttact ggggtgccaa tcaagatttc ttatgattca
     5701 aagcgctact atatccatgg atatgaaaat gcagtgactg tcaaactttc cagtgcaaac
     5761 cgagttcagt tgaatgcaga ggcaaacgaa gatacacgga tgttccgcgt cacagcagat
     5821 atcactaagc tgggcgaagg aacacatgag gttccgctaa aaattcaaaa tttgagcagt
     5881 gctgtcacag caaagatcga accatcgact attacagtaa cggttgagaa aaaggtaata
     5941 aaagatttcg atgtggaaac gttgattcct tcaacgttca caccatctgg atatgaactt
     6001 gatgatactt ccgttagtcc taaaaaagtc tctatcacta caggtgacaa aacattggca
     6061 gaaatcaaac gagtagtagc tgatgtggac gcttcgatgg tcacagatga tgggattaat
     6121 agtgaggtcc caatacaggc gctgaatgca gcaggggaag tattaagtat cgtctctgat
     6181 ccagtgcaag taacagtgag agctgattcg atcaagccaa gcaagtcggt acgactttat
     6241 gggatacagc aaggaacacc agctgccggg gtagaaagct atgatttcag tttttcaaaa
     6301 ctggaagcag aagtaagcgg ctctagcgat ttgcttgcat cgatcggtga cagtattgct
     6361 gtaccgatca atgtctcagg tatcaatcat cgaacaactc gtaaaattga aataccagta
     6421 gaagaggggt taagtatcaa gcccgaatca gttagtgtgg agattacacc cgtgttgcaa
     6481 acgtcaagca gtagctctgc ttcgacgagc agtcggacac aagaaaatac aagtacgagt
     6541 agaagtattt catctgctga ttcaggaacg gaaccatcga caacaaccat ttcttccagt
     6601 tcggaatctg ggacaagcag ctcgaccaca agtacagaag aaacatcaga aacaagcgaa
     6661 tcaacacaaa tgagcagaac aaattcctca gaaaattaag ttaaaggagt aattttacaa
     6721 ctatgggtaa atattttgga acagacggcg tacgcggtga agccaacaag gaacttacgc
     6781 cagaacttgc atttaagctg ggacggtgcg gaggttatgt cctaagccag catgaagaag
     6841 aaggaaaacg cccacgcgtg ttagtagggc gggatacaag aatctcaggt caaatgttag
     6901 aagaagcgct gatttcagga cttttatcgg tgggtatcga agtgttccag ctaggtgtga
     6961 tctctacacc aggtgttgct tatctgacac gtttgcaaaa agcaagcgca ggggtcatga
     7021 tttctgcttc tcataatcca gcacaagata atggaatcaa atttttcggt gcagatggtt
     7081 ttaaactagt agatgaccaa gagctagaaa ttgaagcatt attagatgct gaagtggatg
     7141 aattgcctcg tccatcagct gaaggattgg gaactgtaga agaatttcca gaaggcttat
     7201 tgaaatattc tcaattcctt gtacaaacga tcaacggtga cctttccggt ttgaccattt
     7261 gtgtcgatgc agcaaacgga gcaacttcaa caagtgttaa tcgtttgttt gccgatcttg
     7321 aaacagattt ttataccatg ggcacaaatc cagacgggtt aaatatcaat gatggcgtag
     7381 gctctaccca tccagaacgt ttagctgaga tggtcgttga aaaaggagca gatgctggac
     7441 tagctttcga tggcgatggc gatcgtatta ttgcggtgga tgaacttgga aacattgtcg
     7501 acggtgataa aatcatgttt atctgtgcta aatacttggc agaaaaaaat cgattgaaaa
     7561 aagatacgat cgttacgact gtcatgagta acttaggatt ccacaaagcg gtggaaggaa
     7621 tcggtttgaa agatgtcgtt acacaagttg gtgatcgtta cgttgtagaa gaaatgcgca
     7681 aaaacgatta taactttggc ggcgagcagt ctgggcacat gattttctta gattttaaca
     7741 caactggaga tgggatgctt tcaggaattc agttactgaa tatcatgaaa cagacaggta
     7801 agaaattatc agagcttgca gcagaggtaa caatctatcc gcaaaaatta gtgaatatcc
     7861 gagtaagtga taaaaacggt gcgatggatg ttccggcaat caaaaaagtg attgaagagg
     7921 cagaagctga aatgggatca gaaggaagaa tcctagttcg tccttccggt acagaaccat
     7981 tacttcgagt gatggcagaa gcgcctactg atgagaaagt taattactat gtcgataaaa
     8041 tcgcggcagt agtcaaagaa gaaatcggac tggattaaga gatattttca taaaaaataa
     8101 aagaacaagg accagtgtca gatgtctgac actggtcctt gttcttttat tattctactg
     8161 tcactgattt cgctaaatta cgtggtttgt caacgtcgaa tccccgtaat agcgttgcgt
     8221 aatatgcaag taattgagtc ggtacgactg tggttagagt agttagataa gggtggacag
     8281 ttggtagcac cagctggtca ctttcctttt ctaatccctc tgagacaata actagtgttt
     8341 tagcgccacg gctttcgact tctttcaggt ttcctcgagt atgcgcacca gtcacttctt
     8401 cactgatgat tccaatcact ggtgttcctt cttcgatcaa agcaatcgtt ccatgtttca
     8461 attcgccagc ggcaaaacct tctgcctgga tataactgat ttctttcaat ttcaaagcag
     8521 cttcaagaga aacgttgtag tcagcgccac gtccaatgta aaaggcattt cttgtatcgc
     8581 tgagatatac ttctgccaac tcttctaaat aatctttttg ggcgatgacc gattccatcg
     8641 cattagcaac taaactcaat tcatgagtga tatcgaatac aagagaagtt tcaaattctt
     8701 ttttatcacc gatagctttg gctaataaag ttagtacagc tatttgtgca gtataagctt
     8761 tcgttgatgc tactgcgatt tcgggtccag catgcaatag cagtgtaaag gatgcttccc
     8821 gcgaaagagt agaaccagca acatttgtaa tcgtaagtga agggtagccg agacggttga
     8881 tttttaccag aacttggcgg ctatctgctg tttcgccact ttgactcaag aaaataaaga
     8941 atggtttttt tgaaagaaga ggtgtgttat agccaaattc actggctaaa tgaacctcaa
     9001 cagggatctg tgtcaatttt tccagagtct gtttacctgc taaccccgca tggtaactag
     9061 ttccgcaagc aacgatatag attcgatcgc tatcagacaa tgtatcgacc agcttttgat
     9121 caagagagac acgattgtct tctgttacgt atttttgagc gatacgacgc atgataatag
     9181 gctgttcgtc gatttctttc agcatataaa aaggataggt acctttctcg atatctgaag
     9241 aatctacttg tgcttcgaat gaagaacgtt ctacgatgtc acctgcaaaa ttttgaataa
     9301 taactttttc tgatgtgacg gtaaccatct cttcatcttc aatctcaacg aattccttcg
     9361 tatgagcaag catagccatt gcatcgctgg caatcacatt gaatccatcg ccgagtccga
     9421 ttaatagtgg acttttattt ttagcgacgt aaataacatc aggtgctgtc cgatcaatca
     9481 aagcgaaagc gtaagaacct ttgatgaccc gcaaagcttt taaaaacgcc tcttgcgttg
     9541 tagtatcctt cgcgaaagtt tcaattagat gagcgataat ctcagtatcg gtctcaccaa
     9601 taaagtggtc atttgctaaa taagcaattt taagttcttc aaagttttcg attacgccat
     9661 tgtgaacgag cacgaagcgt ccactttggg aagtatgtgg atgggcgttt tcttcagaag
     9721 gttgcccgtg agttgcccag cgtgtatgac caatacctat atttcctgta gtatcaatat
     9781 ttattttgtt tttcaaattt tgaattctgc cttgtgcttt gaccagataa tcagttgtgc
     9841 catctgagac aaagatacca gctgaatcat aaccacggta ttctaatttt tctagaccgt
     9901 caatcaatcc tggtgttaca ttttttaatc caatcattcc aacaattccg cacataaaaa
     9961 acatcctact ttctataatg aattcgtatg aatgcttcca gctgcaggaa aatccttttg
    10021 ttttatcttt gcagatattt tttgtggatt ttctgtagag tgcagtccct agggccatcc
    10081 gccgaatctt tcgataaacc ctctcctcgt catctgttta cacagacctg gcgctatcct
    10141 aaaaggtata actatcctcc tcttttgttt tttatctgga aacatttaaa atcataacct
    10201 tttgcgaatg ttcttgtcaa caaaaaaaga ataaaaaata aattggtata tatcaaatgc
    10261 tttgtcaaat gctcttttta tgtcaaaaaa taatgggttt ttatctatca tctttttcag
    10321 gagttcggta caatagaaag taatcggata gaggaggagt aaaaatggat tcaaaaattg
    10381 agatcatgac actgggaatg ttaaaaaaac aattgtccaa attcgaagca tcagctggtg
    10441 tatcagatga tacgaaaata tttttagata ctggctggga tagcatccaa gaaatcgcac
    10501 ctgatgcctt ggaagttgct gaagctcgtg agtttacggt agaagatgaa ttgacaaaag
    10561 agtcgttttc cgggtatgcg cgggaagaaa aagcagagcg gttcgacact tctgagcagt
    10621 cagaaactgt gatcgtcata aaaaacttgt attagaaagg agaaagaatg aataagagac
    10681 gttgggcagc agttctgatt gctgcaggat tatttgtggt ttcgttagtt tcagctagtt
    10741 tgactaagcc tcaggaaaac gatcaaattg agggattgaa cagttggtta tatggggatg
    10801 aagaactagc ttcaaatgtg ttggaagagg gagacagtac gcaaaaaatc gtaaaattat
    10861 cagtagatgg aacgattgct gcgggagggg cttcaggttt atttgcttct gaaggttaca
    10921 atcataaaaa ttttatggaa caactgaagg cgattgaaga agatccaaca gtcaaaggta
    10981 tctttttaga agtaaattct cctggtggcg gtgtctacga aagtgcggaa atcgctaaga
    11041 aattggatac gattcgtaaa gaacatgata tcccaatgta tgtcagcatg aaaaacatgg
    11101 cagctagcgg tggctactat atttctgctc aagcagataa aatctttgca acagaagaaa
    11161 cagtgaccgg ctcaattggt gtgatcatgt caggattgaa ttattcagga cttctagaaa
    11221 aactgggtgt ggaagataca acagttaaaa gcggagcgtt gaaagatatg ggttcaggaa
    11281 cgcgaccaga gacaaaagaa gaagaagcgg tgcttcaagc atatatcgac agcgcatata
    11341 gtcgttttgt agcaatcgtc agcgagggac ggaataaatc tgaagaagaa gtcaaaaaaa
    11401 ttgctgatgg aaggatttat gatggtgtcc aagcaaaaga agtaggttta gtcgatgaat
    11461 tgggatttcc tgaagatgct ttaaaagcta tgcgaaagga acaacagctt gaagatgcgg
    11521 aactagtaga atattcttcc acgccatctg ggtttgctag tacatggtta ggaagtaaac
    11581 tagcagaatt ccagggattg aagccttctg agactagtca gatcttatcc gttcttgaga
    11641 atctagggac agcggaatca ccaaaagcaa tgtattatta cggaggtgag taagcatgtc
    11701 agagctttca aatgaaaata aggaacaaga aaaagataag caacagcagc ctgctgtagg
    11761 acctgactcc aacttttccc aaaaggtact gagctctttt gaaagagagc ctttgacgcc
    11821 cgaagaaatc aaaaagaaac aacgtaaatg gaaaaagtat acggacacaa gaaatgaacc
    11881 gccaaaagca aatgattttc ctaagtattt ctatgcaggt ttttttatcc gaatgtttgc
    11941 tttcttggtc gatcttttgt gtatcggagc tattacgcgt ttaacacttg gtattgccaa
    12001 aaatctaggt tggcttaatt tttcagattc ttatctgagt atctacggat tcacagcctt
    12061 acttatttat ctagcgtatt ttattttatt gacgaaattc aatcatggtc aaacgattgg
    12121 gaaaatgatc tttggtattc gagtcatcag ctttgatgaa gaagaactta gctggtcaac
    12181 cgttttagtt agagaaggtg cttgccggtt cattctaaaa tttcctttgc tgatgttagg
    12241 gtatcttcca gcaatcttta atcagaaaaa gcaacatcta ggcgattttt tcactaatac
    12301 aagtgttgtt acgatcaatc tgatcaaagc attcaaccag gaagtcaaca gctgatgagc
    12361 aataaaaagc agtaacagtt gaaagcacct tctaaaagtc agatctaaaa tctagctttt
    12421 agagggtgct tcagttattt ttaattgtca tgcttttttt gttttgctac tattgaacat
    12481 gagctgaaca tgagcaagag gaggacactg cgtgaagccg attgattttc cagaaaaata
    12541 tgagaattta atgcgagtag cacaacaagc actagcaaac cagcaatatc agcaggcaaa
    12601 agaattattt caacgagctt acgagttgaa agaaagcttt gaggctaata gcctgcttgt
    12661 tttttgtctt tacgagttgg acgagaaaaa agaagcattg aagcaagcac tcttgcatga
    12721 aaaacagtat cttcaaaatg aggagtttgc tgaattttat tttgacttgt taatcagtac
    12781 acaagatttt ttatatgcta gaaaattgat agcaagtaca gatttttatg aagctttcga
    12841 gcagcgggtc attgaaaaaa tccaatttgc agaagaatta tccggacaaa tggagaggca
    12901 gaagatcaag gcactccaca ataaatcgga ggaacttcct tcgttggaac ctgcacgtca
    12961 gcttgcgtct attgatgaga tcgaacagct tccttatcat gagtttatcc aaacagcgag
    13021 taaacttatt gtatcgccag aggtccatat tttagcaagg gcaaaattac tggagacact
    13081 taggcaattg aacgaatgcc atcctgtttt ctatttgacg attgaagaaa agttagtcaa
    13141 agtcatacca aaagatctgc caaagccgca gcagcaggca agttaccagc aattatgtgt
    13201 attttcagat cgttacggta atgaagatgc gctgcttagt tccgtgttaa atgaagaatt
    13261 cacgttgcaa tctgcaattg tttatcctgt ctatgataca tacatcgaag acccgaagcg
    13321 ctggtttcaa ctgactgtag aggcgtgtac aggaaaaacg atgtatgaag gtacagaaga
    13381 tgaaaaacaa gattttttaa aaaaaagaga aaaaattttg cagcaaatga tcttcttcca
    13441 ttagatttta tcagtgaaaa agataaaaat gttatgaaaa acaaggattc tatccattca
    13501 atatgtttac aatttgaaag ggttagtgta ctatttaaag gtatgcatga aacgtttttg
    13561 atgtataaaa tgaatttcta aactcggagg gaattaaatg actgcaacat ttgaaaaaaa
    13621 aggcacgaat gatggtgttt taaaatttac gattgcgcaa gacgaaatcc aaaaaggatt
    13681 aacaactgcg tttaataaag tgaaaaaaag cttgaacgta cctggattcc gtaaagggaa
    13741 agtttctcgt caagtattca accgtatgta cggtgaagaa gctttatatg aagatgcatt
    13801 gaatgctgta ttaccagaag cttatgaagc tgctgtcaaa gaagctggca tcgatcctgt
    13861 ttctcaacca aaaatcgatg ttgaaagcat ggaaaaaggt caagactggg taatcactgc
    13921 tgaagtaaca gtgaaaccag aagtaaaatt aggacaatat aaagatctag ttgttgaaaa
    13981 acaagaccgt gaagtaacag atgctgacgt ggatgcacgt atcaaacgtg aacaagaagc
    14041 acaagctgaa ttagttatca aagaagatgc tgcagctgaa aatggcgata cagtagtaat
    14101 cgacttcgaa ggctttgtag atggcgaagc gtttgatggc ggaaaaggcg aaaattactc
    14161 acttgaatta ggttcaggct cattcattcc aggttttgaa gatcaattag ttggtcataa
    14221 atcaggtgaa agtctagatg taaatgtaac tttcccagaa gattatcaag cagaagatct
    14281 tgctggaaaa gaagcagtct tcaaagtgac aatccatgaa gtaaaagcaa aagaagtgcc
    14341 tgaattagat gacgaattcg caaaagatgt tgatgacgaa gtatcaacat tagacgaatt
    14401 gaaagaaaaa tatcgcaaag aattagtaga aacaaaagaa aaagcagcgg acgatgcaaa
    14461 agatgaagca gcgatccgta tggctgttga aaacgcagaa atcgttgaat tgccacacgt
    14521 gatggttcat gatgaagtac atcgttcaat ggacgaattc ttgaacaaca tgcaacgtca
    14581 aggtatctct cctgaaatgt actatcaatt aactggttct acagaagaag acttgcataa
    14641 acaattcgaa ggtgaagcag aaatgcgcac aagaacaaat cttgtgatcg aagcaattgc
    14701 agctgctgaa aatcttcaag caacagaaga agaaatcaat gcagaaatcc aagaattatc
    14761 agaaacatac aacatgccaa tcgaacaaat caaacgcgtg ttgacagaag atatgctgga
    14821 acatgatgtg acaatgaaaa aagcaatcga attgatcaca ggtacagcga cagaagcgaa
    14881 agaatagaaa tcaagtaata aatcatttct gaagaagcta cttctagaaa attgtttatt
    14941 cgaaataagg gactgtgaca cgattttgtc acagttcctt attttttgtt aagagtaaaa
    15001 aatgcaaagg tataggtcag ttgatccttt gttaaacact ttttcttttt actaaactat
    15061 gttatgatag taaaagactg taaagtcttt gatagtttct tgcatacttt ttgcaagtta
    15121 aggggtgaag accatgtatg acaatccaag tagcaatgag accgtacgct gttctttttg
    15181 cgggaaaacc caagaagaag tgaaaaagat cgtagctggt ccaggggtct acatctgtaa
    15241 tgaatgtatc gacctatgta aagaaatcat tgatgaagaa ttttatgaag aagctgtacg
    15301 cgaattcact gaagtcccta aaccactaga aattcttgaa gtattgaata actacgtgat
    15361 tggacaagat cgcgcaaaac gctcgttagc tgttgccgta tacaatcact ataaacgtgt
    15421 caatcagcaa gcacaagaaa acacagatga tgttgaactg caaaaaagca atatctgctt
    15481 gatcggacct acagggtctg gtaaaacatt tttagcacaa acattagcaa aaacattgaa
    15541 tgtacctttt gcaatcgcag atgcgacaag tttgacagaa gctggttatg tgggggaaga
    15601 tgtagaaaac attttgctta aattgcttca atctgctgat tacaatgtgg aacgtgcaga
    15661 aaaagggatc atctatattg atgagatcga taaaattgcg cgtaaaagcg aaaatgtatc
    15721 tattactcgt gacgtttccg gcgaaggtgt ccaacaagcc ttgctgaaaa ttctagaagg
    15781 aactgttgca agcgtgccac ctcagggagg acgtaaacat ccacatcaag aatttattca
    15841 gattgatacg acgaacatat tgttcatcgt aggtggtgca tttgatggca tcgaaacgat
    15901 tgtgaagaat cgtatgggcg aaaagacgat tggatttggt acacaaaacc aaaaactttc
    15961 tgaagacgaa agtgtcatgc agcatatcat tcctgaagat ttactaaaat tcggattgat
    16021 ccctgagttt atcggacgat tacctgttat ggcagcttta gagaaattga caacagatga
    16081 tctggtacgg atcttgactg agccgaaaaa tgcgctagtc aaacaatatc aaaaacttct
    16141 ttctctagat aataccgaac ttgaattcga acctgaagcg ttgagagcca ttgcgaaaaa
    16201 agcaatcgaa cgcaataccg gtgctcgagg acttcgttcc atcattgaag aaatcatgat
    16261 ggatgtgatg tttgatatcc cttcaaatga aaacatcgaa aaagtgatta tcacaaaaga
    16321 agcagctgaa ttgtctggtg agccaacagt aatctataaa gattcagaga aaaaagcggg
    16381 ttgatgaaaa gaggctgccg taaaagtcat tttggctttt gcgacagccg cttgctttat
    16441 tcatcacctt gttaaggaga gagaccatga aagtccatca agctgaaatt atcataagtg
    16501 cagtggcacc aaaacaatat ccagatacac aattgccaga aatagctctg gcaggacgat
    16561 caaatgtagg gaaatcttct tttatcaaca cattgattga tcgaaagaat ttagcccgta
    16621 cttccggtaa gccaggaaag acccagacat tgaattttta tttgatcgag aacgcattac
    16681 attttgtcga tgttccggga tatgggtatg caaaagtatc caaaactgag cgagcaaaat
    16741 ggggaaagat gatcgagaca tatttaacga ctagagacca gctacgtgcg gtcgtttctt
    16801 tagtagattt gcgtcataag ccttctcaag aagatatcca gatgtatcaa tttttgaagt
    16861 attatgatat ccctgtaatt gttgttgcca ctaaggcaga taagatccct cgtggtaaat
    16921 ggaacaagca tgagagcgaa atcaaaaaag cgttggattt tgatcgcaat gacacgttta
    16981 ttctcttttc ttccgttaca aaacagggaa aagaagaagc gtgggcagca atcgaacaag
    17041 caattgaatc ataaaaagaa taaaaaagac ttaccagtca aactctttca taaaagttt
//
LOCUS       DAYYKN010000056        16923 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000056 DAYYKN010000000
VERSION     DAYYKN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.056"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(246..2138)
                     /locus_tag="ACHBB7_001546"
     CDS             complement(246..2138)
                     /locus_tag="ACHBB7_001546"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209504.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2135..3862)
                     /locus_tag="ACHBB7_001547"
     CDS             complement(2135..3862)
                     /locus_tag="ACHBB7_001547"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209505.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3878..4528)
                     /locus_tag="ACHBB7_001548"
     CDS             complement(3878..4528)
                     /locus_tag="ACHBB7_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM9209506.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4732..5028
                     /locus_tag="ACHBB7_001549"
     CDS             4732..5028
                     /locus_tag="ACHBB7_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9209507.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5200..5820
                     /locus_tag="ACHBB7_001550"
     CDS             5200..5820
                     /locus_tag="ACHBB7_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9209508.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5912..7078)
                     /locus_tag="ACHBB7_001551"
     CDS             complement(5912..7078)
                     /locus_tag="ACHBB7_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9209509.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7090..7443)
                     /locus_tag="ACHBB7_001552"
     CDS             complement(7090..7443)
                     /locus_tag="ACHBB7_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM9209510.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7571..8593
                     /locus_tag="ACHBB7_001553"
     CDS             7571..8593
                     /locus_tag="ACHBB7_001553"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM9209511.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8612..8773
                     /locus_tag="ACHBB7_001554"
     CDS             8612..8773
                     /locus_tag="ACHBB7_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209512.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9032..9466)
                     /locus_tag="ACHBB7_001555"
     CDS             complement(9032..9466)
                     /locus_tag="ACHBB7_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209513.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9576..11435)
                     /locus_tag="ACHBB7_001556"
     CDS             complement(9576..11435)
                     /locus_tag="ACHBB7_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9209514.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11436..11576
                     /locus_tag="ACHBB7_001557"
     CDS             11436..11576
                     /locus_tag="ACHBB7_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM9209515.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11553..12713)
                     /locus_tag="ACHBB7_001558"
     CDS             complement(11553..12713)
                     /locus_tag="ACHBB7_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9209516.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13020..13931)
                     /locus_tag="ACHBB7_001559"
                     /pseudo
     CDS             complement(13020..13931)
                     /locus_tag="ACHBB7_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(14097..15290)
                     /locus_tag="ACHBB7_001560"
     CDS             complement(14097..15290)
                     /locus_tag="ACHBB7_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9209517.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(15374..15574)
                     /locus_tag="ACHBB7_001561"
     CDS             complement(15374..15574)
                     /locus_tag="ACHBB7_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM9209518.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(15998..16231)
                     /locus_tag="ACHBB7_001562"
     CDS             complement(15998..16231)
                     /locus_tag="ACHBB7_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9209519.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(16238..16669)
                     /locus_tag="ACHBB7_001563"
     CDS             complement(16238..16669)
                     /locus_tag="ACHBB7_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9209520.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         4751 a         3696 c         3178 g         5298 t
ORIGIN      
        1 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
       61 aggttgtaaa atcagcggtt tgacctgagt attagagata caaaacggaa aaatagcttc
      121 tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca ccgtttattc
      181 ggaaaaaggg actgtgacac gacttttgtc acagccccta aaaaaaataa taaatttgta
      241 ctattctatg aagcttcttc ttcagaaaac tgactgttat aaagatctgc atagaatccg
      301 tctttttcca tcaatgtatc gtggtttcct gtttctacga tcgaaccatg attcatcaca
      361 ataatgttgt ctgcatcgcg tattgtagac aagcggtgag caacaacgaa acttgtccgg
      421 ttttccaata ggcggttcat cgctttttga atcagtattt ctgttcgtgt atctacacta
      481 gatgttgctt catcaaggat caggacatct ggatttgcta agaatgcccg cgcaattgtg
      541 attagctgac gctgcccttg cgaaatattg cttgcatcct cgtttaatac agtgttatat
      601 ccttctggca atttacgaac aaaatcgtct acgtgagcag ctttagcagc ctcaatgatt
      661 tgttcgtctg ttgcatcgtc gttcccatat ttgatattgt catagatact tcctgtaaac
      721 agccaagtat cttgcagtac cattgaaaac tgtgcgcgca attcatcacg tgaaagatca
      781 cgggtatcta caccatcgta acgaatacta cctccagaaa catcgtagaa ccgttcaaga
      841 aggttgatca gtgtcgtctt ccctgcacca gtcggaccta caatagcgac catctcacca
      901 ggtttgacat ccaagttaaa atcagtcatt agcaagttat ctggtgtgta gccgaattga
      961 acattttcaa aacgaacttt gtatggtgta tctttttctt ctggaaggcc agatttttca
     1021 ttggacatct cttcttcatc caacacttca aatacccgtt cggctgaagc aactgttgat
     1081 tggatagtgt tcatcaaact tgcaagttga gtgattggtt gagagaactg tgttgtatat
     1141 tgcaagaacg cttgaacatc ccctaatgtc atgttcccat ttgcaacttt tactccgcct
     1201 agtacagcga cgaacacata gcctagattt ttaacgaaat tcatcaacgg catgatgatt
     1261 gccgaaataa attgagcttt ccaaccagcg gcatacagat tctgattttc tttttcaaat
     1321 acttcctggt ctttttcagt atgattgaat gttttaacaa tggtatgacc accatatgtt
     1381 tcttctactt ggttattcaa taaacctaaa cttttttgct gatctgcgaa aaacacttgt
     1441 gatttcggcg ctacgatcat cacgatgatc aagcttaaag ggatcatcaa taacgcgact
     1501 aacgtcaatt gccagctgat tgaaagcatc atcacgatga ccccaatcaa tgtgactgca
     1561 ctagttacaa attgagtcaa attttgctgc agcgtgcttg cgatgttatc catatcattg
     1621 attgctcgag acatgatatc cccatttgta tgtgtatcat aataggaaat cggtacacga
     1681 ttcattttgg cttccaaatc atgacgtaat tcataaactg ttcgttggga aacacgtgtc
     1741 ataataaatt gctgtaaaaa gttgaataca gcagaaatca aatacatcac gattactgtc
     1801 gcaatgatct gcgcgatttt atcaaagtct atcggaaatt ttgttacttg ttggccttgt
     1861 tgcatctgtt gagcgccagc cattacacct ttgaatattt ctgtggttgc tttccccaag
     1921 acctttggtg tttggatttg gaagatcacg gctgctattg ctagtacaaa tacagcgatg
     1981 atcgcaacca tacgtttaga catatagcca aacaatcggc gtaccgttcc ccagaaattt
     2041 ttaggctttt caccttttac accgagattt ctgccaggtc caccatgtcc tggaccgcct
     2101 gctggtccat gagagggacg tgtgtttgtt ttactcatgc taagtcctcc tctcttagtt
     2161 gtgaatgaac gatttcttga taagtttcgt ttgttgctaa tagttcttca tgtgttcctt
     2221 tgccaaccag ttttccttca tctagcacaa ggatcatatc tgcatccatt actgtactta
     2281 ctcgttgagc aactaaaacg gtgatacttt ctttcatatt cgttttcaat gcttggcgaa
     2341 gattggcatc cgttttgaaa tccaacgctg agaaggaatc atcaaacaca tagatatctg
     2401 ctgttttgac taatgcccgt gcaattgcca gacgctgtct ttgtccacca gagaagtttc
     2461 ctcctccttg ttccacatgg ctatctaagc catcttccat ctctgaaaca aaatctttag
     2521 cttgtgcgat ttctaatgct ttccaaatca tttcatctgt tgcatccggc ttaccatact
     2581 gcatattgtc tcggatcgtt ccagagaaca gaactgcttt ttgaggaaca aagcccatta
     2641 attctcgcaa attatattga gtcatgtcac taatgtccgt tccatttatt tggatcgaac
     2701 cagattcgat gtcatataat cgagggatca aattgactaa ggtcgtttta cctgaaccag
     2761 tcccccctat gattgcgacg atctcgcccg acttggcttg gaaatcaata tcttccaatg
     2821 caagtttttc tgctccatga tatcgataat tgacatgatt gaaggccaat gttgcgtttt
     2881 ttccagcaaa agaaactgtt ttaggatttt cagggtcatg gataccgatt ttttcatcca
     2941 acacttcatt aatacggtca gctgaagctt gtgcacgagg caccatgata aaaatagcgg
     3001 aaagcatcat aaagctgatc aagatctgca tcgcataagt cataaatgca atcagattac
     3061 cgacttccaa agtcatatct gctatgtagt gtccaccaaa ccaagtgatt gctacgtttg
     3121 taccgctcat gatcaatgtc atcattggca tcatcaatgc gacgatcgta ttaactttga
     3181 ttgccgtatt tgtataatct ttattcgcta aatcaaaacg attttcttca aagcgtgtct
     3241 tatcgaatgc tcgaatcact ctgacccctg tcaatccttc tcgaaatacc aggtttaaac
     3301 ggtcagtttt cttttgcata cttttgaata atggaacagc aaagtacatg attccgccga
     3361 tcaaaataat cattactggg atgacaacta agaagatttt tgtcaactga tgatcttttt
     3421 gataagcaag tatgctcgcg ccaatcaacg tgattggtgc attgatcatc atacgtaaaa
     3481 acatttgtgt aaccatttgg atttgagtca catcattggt cgttcttgtg atcaaagagg
     3541 ccgttccata tttatcaaac gcatcttttg tcagactctc tgatttttta tagacatccg
     3601 tacgcaattg cttacccagt ttctgtgatt cacgagtagc aaaaaatgta tttgcgatag
     3661 cagctaagat actgatcaaa gaaaagccga tcatcacaaa acctgtatgc caaatataat
     3721 cgacatctcc ttgcgctacc cctttatcaa taatattgga ggtaagtgtc ggcaaatata
     3781 aatctgccag tacttggatc accatgaaaa ctgcagctgc aattgctgaa gtcaaggaga
     3841 tccgttttac gatttttagc atctagttcc cttctttcta tttatatact ccgtttttca
     3901 gccaagaaag tttcaaattg aattcactga ttgcttgctc tactgagtaa tcttcagatg
     3961 ttgaaaataa gcgatacgaa tgcgcaattg ttgtaaatac agtattcatc actaattgga
     4021 tcagtaattc caattcatga tcatttttta ctttcagctt cgtccgatcg atcaattcaa
     4081 gcaactcttt tgcatcttgt ttgtgttctt tatgtggatc gtgaggcgtt ttttggcgac
     4141 tcgtatgagg agcgactttt cttgaggcac ggtaatccat gttcataaac aagttacgat
     4201 aaaaagaagc attctcgcct tccaaaatct cataaatcat tttagaaaaa taggattcga
     4261 atcctttgaa tagatcacca tcggattctt tcattagttg taacatatct ttagagctgt
     4321 cacgtctcat tgtctcaaag taataaaaat aaagatcctc tttatcttca aaatactgat
     4381 agaaactgcc tctagggata tccgctaatt tgacgatatt agcaatcgaa gcttctttta
     4441 agggtactct agaaaactcg attctcgcag cctcgattag tcttttttgt ttttctttag
     4501 gcaaatggaa aaatgtctgt gtcggcatat ggatcctcct ttctttcaag atgacacatt
     4561 gtcatatgac gctgtgtcat tataacacct cctacctgtt ttgcaagaca tttgcatagt
     4621 ttttttcatt atcatccgat acattaaaaa agtgataaga ggaatccttc catttgaatt
     4681 aatgagagtt gcccattaaa atagccacat actaaacaaa ggatgagata aatggcccaa
     4741 tcaattagtg tccaagactt caataaattg cctctcgatg ctgacagcat cgttttagat
     4801 acaagaaata ccttagatta cacaaaaaac cacttaagcc actcgctttc tatccccgct
     4861 gctatcttgc caagacaatt aaaaaatcta gatccaaaga aactttatta tattctaagc
     4921 tatacaggca ggcgatcaga agcattagcc gatcaactta ctgctaatgg ttttcgtgct
     4981 gtttatatca ttggtgggat ggaagctctt cagtcgagtg ttgcttgaat attgcttgtt
     5041 agattcaaaa gaggctgtga caaaaaatcg tgtcacagcc tcttttatac cctgtctttt
     5101 tttagttatt tattcttcaa ccttatcaat atgacttgat agccttttct ttttctcata
     5161 gttataacaa tcatagcaat aaaaggagaa ggtaagcata tggcatctgt tttagtagtc
     5221 aaaggtcatc ccctaactgc agaagaatcg cgcacagtta aggcattgac gtctttttta
     5281 acaagttata aagaaaatca tccagatgat gaagtgactg ttttggaact ttatcgcgat
     5341 gatattccgg aaatcgatga agaattatta tcaggttggg aagcattacg tgctggtgca
     5401 gaattcactt ccttatcaga aagtcaacaa caaaaaattg ctcgtttcaa tgaattgact
     5461 gatcagttcc ttgctgctga taaaatcgtt atcgcaaacg ccttatggaa tctaaatatc
     5521 ccaacaaaat taaaagcttg gttcgatacc gtcaatgtag ctggaaaaac gttccgctat
     5581 acagaaaacg ggccagaagg attagtgaca ggtaaaaaag cactgcacat ccaatctaat
     5641 ggcggtgtat acaacggaca agactttgcc tctcaatatg taaaaggtat tttaaacttt
     5701 gtaggcatcg atcaagtaga tcagctattc atcgaaggaa tcgattatga ccctgaccgt
     5761 gcagatgagt tgatgcaaaa tgcattagat aaagctgctg ctttaggtaa atcattttaa
     5821 aaattgagat aaatgcatac aaaagagaca gaggaaaaaa tcttcaaagc agcatttgta
     5881 ccttggatat tttcccatct gtctcttttt gttattcttt ctgctttttc aacggatttt
     5941 cttcataaag agaagagatt gttgtgtacc attcaaaaac atgagtaatc actactttag
     6001 ctgctgcata aacaggaatc cccaagatca caccgatgat tccgaacaat ttcccagaag
     6061 tcagcagaac taataaaatc gttactggat ggattgctaa ctgattccct agtactaatg
     6121 gagagatcaa gcgtccttct attgtctgct ctactgcaaa aacgatcagt actttgatga
     6181 tcatgaatgg acctgctaca atggctaaaa agatagccgg aatcatcgct aaaaatgatc
     6241 ctaagtaagg gatcaggttc aaaaaacccg caataatccc caacgtgaca gcatactcta
     6301 aaccaatgac cgcaaaacca atcataaaca tgattgccac agcaaaagcc acagttagtt
     6361 gtcctcttat ataagaagaa acttgactat tcatttcact cagcacattc aacgtcggtt
     6421 tttgcatgcg tgtcggtaaa aatttgacaa aataaggtgc taattgttta ccgtctttca
     6481 atagataaaa taagataaac ggcatcgtca gcagagccac taaaatagtc gctaccgcgc
     6541 cgacaaaact tccaagactc tgaactgtac tcttagatat atcttggatc atatcgcctg
     6601 ccgaattcat gaacttatca cctgctgttt ccaactgttc gcgaaattgg ttgaacagcg
     6661 ggtcccgcaa aatctcggtt aatttattat caatcgtatc tacatattga gggaagttac
     6721 tgatgaagct cactgtttgt tcctgaatct tagggatgat cactacgctc ccccatgcga
     6781 ttagtgcaac aaccaaaaca aacagcccaa tgatactgta aagccttggt acttttcgct
     6841 tttcaaagta atcaactacc ggattcatca agtaatacaa gattcctgct aaaatgatag
     6901 gcagacctac gattgctaaa aattgccaga ttggtgcaaa caaatatgaa acttttgtaa
     6961 ataaaaagac gatcagcaat attaacaata caacaagtaa ggctgtgact acttgattat
     7021 ttaaaaacca gcgccaaaac cacgataccg gtctgttctt cttatttttt ccttccataa
     7081 ttcctcctgt tagttataaa aaatttggct tcccaaacca atagcaggca gtctatgcgg
     7141 atgaagatag attccatttg caatttgtcc ttcttctgga caagcatcaa aaatcaatga
     7201 tacgtgagct actgtctcta aattcccatt ggccatcgga ccgacataat cgatcgtata
     7261 tacctgatca tcaaaagcaa ttgttccgcc tgctttcaaa gaaaaaggtt cgtcttttga
     7321 tacttcttga atcaccgaat attttcgtaa ggcagctgtt gctgttttat cgaacaaaat
     7381 cagcatgggt tcttccttgt ttattgcttc tgtcccaatt tctgtaatct ttgcctgaat
     7441 cattgcactc tctcctttaa ttgctttcaa aaacagtata acacattctc attctttagc
     7501 cgtgtggaat aattatgtga tagaataaaa acgaaaacta tttcattaca attaggagga
     7561 atcagctcat atgatcgaaa agataatttt aggaacctat acgcgtcgcg aaagtaaagg
     7621 aatctatacg atcgaccttg atacagaaaa agaagaatta gccaaccttt cattagctgt
     7681 tgaagaaaat agccctacct atgtagcaaa aagtaaagct ggcaatcttt acacagttac
     7741 tagtgtggat ggattaggtg gtgccggtgc gtacgataag gattttcatt ttttgaatgc
     7801 ggtaacagag tctggcgctc ctttgtgtta tgtagcggtt gatgaagcac gtcaattact
     7861 atacggggcg aactatcata aaggtgaagt aaatgtctac catatattag gagatggcga
     7921 actgaaagca gcagattcaa tattccatca agaagcgaca ggtcctcata aaaaccaaga
     7981 tcatgcgcat gtccactata cggatctgac tcctgaccaa cgattagtcg tatgtgatct
     8041 tggtacagat cgtgtgtata cttatgatgt gtccgaaaac ggcaaattga atcttgtaac
     8101 taccttcact gcagagccag gaacaggccc tcgccatctt gtctttcatc caaatggaca
     8161 attcgcttat ttatttggtg aattagacag cacagtccgc gtgctttctt ataataaaga
     8221 aacaggtgct tttgaagaat tacagaaaat atcgaccttg ccagaagaat tcgacggtga
     8281 aaatggaggg gcagctatcc gcatttcaaa tgatggcaag ttcctttatg cttctaaccg
     8341 aggacataac tcgattgctg tattcgcagt ttttgaagat ggttcccaca ttcaaaacaa
     8401 gcaaatcatc tcaacagaag gggattttcc ccgcgatttc gcactagatc caagcaataa
     8461 ttttgtcgtg tgtgccaatc agaacaccga taatctgaca ttatatcgcc gtcatcctga
     8521 aacaggatta ctggaattac tgaaaaaaga tgtctatgta ccagaatgtg tctgtgttta
     8581 ttttgatcgt tagtaagaaa aggagaatga aatgaatcag caagacgtat tagacagact
     8641 acgggaagaa ctaaatattc ctttctttga tgggaaaatc gaagataaag agtatagcga
     8701 agaggaatat cagaaactca aagccgattt acagaactac tttgaacaat atgtacagaa
     8761 tgtagaaaac taatcagctt tccatactat ctgaaaagaa catcaggaca agacttttcc
     8821 gtctatcctg atgttctttt ctacaaataa aaaaccgccg agatggttag actcgacgga
     8881 aaaggagtat ttactcgatt ttgagtaagt acaaggttgt tgttggttat aggtatgtca
     8941 ttatcataaa ggattttgat cactctatca taaaggtaat attacattta tattagataa
     9001 ctctagtttt tattgttatc aaagatttct tttatttttt caacaacaaa ttagtcattt
     9061 tttggatggc agctgtcaat tggtctgtcg cttggtcttg ttttgcttca gatgtattga
     9121 atagatcttc atctacccct gattcaagat cgatactttg gatcatttct tcacagcctg
     9181 ctacatatga aagataagct ttttcaaaat ttttatgaat gcccattgct tttgctggtg
     9241 gacgcaaagt actgactttc ttaagcattg cccgatattt ttttgttcct tcttcaaata
     9301 acaggatgat ctcttcaaga gtcgatttgt ccagttcact tgtttttcct tcatcaattg
     9361 cgtttctgat cacttcatat ttcggatgca ttccttctcc gattgcttct gtttccttga
     9421 cgatttgatc gatggactgc acataatagc caatattcgg taacatgttc gttttcctac
     9481 tttctccatt gttgttatta tttattctac ttgtttttat cctcaaatga caagtacttg
     9541 aaaaaattta atggtttcta tacagctcgc cattcttatt cgtgctcaaa gaaaccatct
     9601 gctaaactat attgtttccc tgcgacaatg tcatattgga tcaattgata ttcgtcgtaa
     9661 atctcttgtt gcgagcgatt gaattgcgct tctttatacc actgtccgtt ttgatagtac
     9721 agttctcgtt caaacgccgg aagttcctgt tctaatgata aaagcagctg ataaaagcca
     9781 tttgttgtct gattcgtctg ttccattaga ttcgctgcaa aataaaacgg actggtgatt
     9841 gcatcgtgag tcgtttgctt ttctaattct cctttactgt cgaacataag aaattgtgtt
     9901 tcatgcaatg tttgtttgtc attttttgct tggatcgtat ctgaatagat ccctggaaga
     9961 tgatcccccc aaaaaacaac taaagtcctt ctagaaagat tcttcaattc ttccgtgaac
    10021 tgcttcagtg ccgtgctgct ataggaaata tcttgcagat aattctctaa gtcaagtgtc
    10081 ccacttccat ctgaagtttt tgcaggatag tctagattat catactttcc atcgtagggc
    10141 atatgtgtct gcatcgtgac taaatgaatg aactgaggtg ttttttcttc cttgagaagt
    10201 gtcattactt ctttgtaagc tgactcatca gaaatatatg gattattctc tatcgtatcc
    10261 gtataggtca tcgtacgctc gctgatgaac tggtcaaatc ctaatgtctg ataaacatcc
    10321 tctcttttat acatcgaagt attgtatgga tggatcgctg tcgtctggta tgattgtgcg
    10381 tttaacgctg aaacgataga aggaagctga tccatttttg gtacaagcat cgtgtaaggg
    10441 gtggtgagtt gcgcattaaa taacgccata gaaaaaccag tcaatgcttc aaattcgata
    10501 tttgctgttc caccgccata gttttgagaa agcatattgc cactatatgt ttgatcagct
    10561 acttcataat aatctgccaa tggctcacca gatacttcta cgccgtttaa tcgactagga
    10621 tctgagaaac tttcactcat cacgaacacg atgttcggcg actcttcctc tacagtttta
    10681 tttttttcat cggctagctt ctggtatttt tcagtgattt ctttgatctt ttctttcgaa
    10741 tatccttcag gttcatccat tggttctacc ttcagattgt acaaaaaccc accgataaaa
    10801 ccagtgttat agtagttcat tttctggctg taaggtatcc atagtgccgt tttattatat
    10861 gcttttcgca acaaattatc gggattgttg aaattactga tataccctag cagtccaatt
    10921 gccacaagca gtgtcgttaa tcggatcaat tggaatcgcc ttcctttttt caggcttcga
    10981 aacatgtacc agcaaaacaa tcctatcacg gttcctgcgg cagctaagat cactgtaaac
    11041 agcatagtgc cggtcatatc tttcagcaat ccgatttcag tgatcatctt caaatcgtct
    11101 ggataaatcg gttcttgccg ataactcatt ttcatataat ttgcaaaacc taatacaccg
    11161 atggctacga catacgtaag ggaacctaaa gggacagatc ctgctagtgc aatcagaaaa
    11221 ataagaataa tcagcaagac gagacaggct aagaagaatt tttccgtatg ccaagaaaaa
    11281 gcaaatttca acgcaaggtc tacggataga tcattttgac accactgcaa aaataaatta
    11341 ctaaaaacaa caagaaaaaa gatactgatg attgctgctg ttccttttaa aatctttttc
    11401 tgtttggtcg aaagtctgct tatatatctt ttcaaatgct tgctccttca tttctatcaa
    11461 taaaagaatg ttgcttctct tattttagtt gtacgaaatt cgaaagtaaa gaacggaaag
    11521 aagaattaat gaaatccaaa tattctcttt ctttatggcc tttctggttt ctttaataag
    11581 cggacctttc gtactccaga aattttttct agctgttgtt tcacttgatt gagctgttca
    11641 aaatcagtac tgtctatgtc aatcaatatg tagacatgct cattcttatg gttacgcgat
    11701 atatctgcga tcgctagctc gttctcattc agcagagaaa atatctctgc taatatattg
    11761 gttttctttt gataaaatat agtgaatcga aacggcgctt ggaaaaacat tcgtgcggat
    11821 ggataattga ccgcttctct aaccgtaccg aacaaaagat aatcttttaa ggcttcaact
    11881 gccaaacgtc cgctgtctgc caaagcttcc tgagtcgatc cccctagatg aggcaacata
    11941 agtatacggt cattttctaa gaactcttct tctggaaaat cagtcaagta tttagccaga
    12001 tgttgttttt ccaatgcttg cataacagca tatttatcaa caatttcact tcttcctaca
    12061 ttgataagta cagcatcttt tttcattttt tctatattcg cctgatcgat caatcctttt
    12121 gtatcttctg tcaacggcag catgactacg atgtaatccg aggtagaaag aactgtttct
    12181 aaatctgctt gccgaacata atccaactgg gcatcgcgga tagaataacc caagacatcc
    12241 atccctaagc tatagcaact tagcgctact tttgtgccta tcgctccaag accaagaaga
    12301 ccgatggttt tgccttgcaa ttctcgtccc acataagcac ttcttttgtt ttctgcctgt
    12361 tctaaaatat taggacctgt tagtgtttgt accatacgac ttgcttcgat tatcggacgg
    12421 gaagataaga gcaaacagca caaaacaagc tcttttactg cattagcatt tacacctggt
    12481 gtattcatga cgatcgtccc gttttcagaa gctttttcaa cgttgatcgt gttcacccca
    12541 acaccagctc ttgaaattgc tagcaaatct ggtgtaagcc attcatcttt cactttcgta
    12601 ctacgaataa ctattccttc ggctggcaaa tcatctatta ttttgaaata ttccccttgt
    12661 aacttttcta tctcttgttc tggaatagca tcaatcaaat gaacgtgata cataaaaccc
    12721 cctccttact aacaagtatc tcgaaatttc tgcaattaga caactgttta ccatttgact
    12781 tcctagaagc aaaaagagca gttagagcag agtaggctga gtgataaaaa taaggctatg
    12841 acaagttact gccataagcc ttattttcga gagcaaaata ttgagggtga gatatcttga
    12901 taaatggtgt tccgttagct gacattgata ataagactaa atttcaaaaa agttgaaaag
    12961 caaatttcgt caatctcttc ttatttctta aaactaagtg gctttttcac aacctctttt
    13021 tatctaataa ccactgtttc caactcaact aattttgccc acgtttcaat ttgatctgtt
    13081 gttagattta gcgagacaac ggtatgatga ccgccacctg cctgaatcca tgaatggaca
    13141 ccttcatgga aattcggttt agttttccat aacacacgtg caacaggtaa attaggtgct
    13201 tcagtcgttg gttcaaaggc ttctacttca ttgatcagca gtttataatg cgtaccgaaa
    13261 tctgccatcg atacgactac cccttctcct gctttcccat caaatacaag acgcgccgga
    13321 tcttctctat ctcccattga taaaggagaa acaacgattc ttggtttcgt cgcagcaagc
    13381 gtcggatcta cttccatcat atgggattcc aagattgctt cttggccact agctagctcg
    13441 taagtataat cttccatgaa tcctgtatct ttccctcgag ccatgatttt cagcaaccga
    13501 tcaatcgctg cagttttcca gtcgccttcc ccagcaaaac cgtatccttc tgccatcaaa
    13561 cgttgtacag ctagtccagg tagttgtttc attccataca ggtcttcaaa attagtcgta
    13621 aaagcgttat agcctccttc ttctaagaaa cggcgaatac cgatttcctg ctgagcttgt
    13681 acttttacat gttcctccca tgtcttttct tcataatcac catagtcgaa ggtatacaat
    13741 tccttgtact cttcaaataa ttgatcgact tcttcgtctg atacgcgatc gattacttgt
    13801 acaagatctc cgataccaaa ataatcaaca gtccaaccaa attgaatctg tgcttccact
    13861 ttgtctcctt ctgtgacacc cacgtttctc atgttgtcac caaaacgagc cacttttatt
    13921 ccaaaattat atgggatttt agattatctt tttagaagtt cttgaatgtc ggtatagccc
    13981 gtgtttccgg gcttttccgg catttttgat attgttagat acttacaaat gttcttatta
    14041 cctctcacca tttccccctc aaaagtagta aatggagtag taaaaccggc cttgtgtcag
    14101 gctgccagcc gttccatttc tgcccttgcg gaatcgaatg tggcgtgtgc ataatagttc
    14161 agcgtcatgg tgatattgga atgtcccata atgtactgca aggctttggg gttcattccc
    14221 gcattggcta accgggtgca gaatgtatga cgcatagcat ggggcgtcgt cgttttcggc
    14281 aacgcttctt catggctctt gttgtacttc ttcacaagcc tgccaaacat cccgccataa
    14341 tctaccgccg tcatgggata gccgttctgt ttgagaaaca ggaaattggc atacccgtca
    14401 ataatgaacg gctttgcacc tttgcggttt tttatcactc gctggaacgc ctgatacact
    14461 tcctcattca tggggatttt tctgacgccg ctttgggtct tgggttcgtc aatatagtaa
    14521 ccgacgccgg tatttctcaa aagctggtgg ctcacgatga ttacccggtt ttcaaaatcc
    14581 aaatctttgt ccgtcagtcc gcacagttcc gaaatgcgaa gccctgtccc cagcagaata
    14641 acgattgcgt cataatgctt ttggtaaacc ttatcacctt gaataaagga cagaaaactt
    14701 tcttcctgtg ctggtgtaag aggcaccttt ggttccgtat cgtcgtccag aacgtcacta
    14761 agctggaagt caaacgggtt ttttcggatg caatcgtcct gtatggcggt gtaaaaagcc
    14821 gctttcaggg aacgcttgtc gttattgatg gtcttataag aaaggccctt ttctttcatc
    14881 cgaagcgccc attcctttgc gtcggacagc ttcacactgt caatcgggca gctccccagc
    14941 cgatcttgtt ccagaatacg catcagccgt tcccggccca gtttggtgtt gtgcttcaca
    15001 ttgccccggt gcctgatctg cttggcgtag agctggcaga cggtcatttt cttgccgatg
    15061 gtgtctatcc cgtcgtcaag gtctttctgg atttctttct ccttttcccg gagtgaaata
    15121 tcctcccgtt tacctgccgg ggtcttatcc gtaggcacta acttccatga gtaaacaaac
    15181 tgcggttttc caaaggtatc aacatatttg taagcatatc gtccgtcttt tctctggctc
    15241 tctccagacc gcaaaacgcg atttttgctg tctcgtcttt tttctgacat ctcgtcatgc
    15301 tcctttccat gacggaaaga gccgcatata tgcttataaa ttattataac atatctgcgg
    15361 ccctttttca ctattagatt gtgtccaaag tgtcaattat tttctcaaac tgcttgcgtt
    15421 tgatctggat gcggttgcca ttcataatga cccagccaga ggccggattt tcctcggcca
    15481 gcttacgcaa tttgttttct ccgatacgga aatatttaga cgcttcctca atcgtcagcg
    15541 tgtatttttc ccaaataggc acatcatggt tcataatgca gccccctttc acaaattcaa
    15601 aggtttgtgt gatttagggg catagacgga cggctgctgg cacagctcca cgggactttc
    15661 acccccgcgt ggttctcacg cggccctacc cattgcctgc gacgctttta acgctcggac
    15721 tgtggctata agggagtatc atagtcctgc ctgtacgtcg tcgcccgcaa gccgccacgc
    15781 tcacattgcg gcgtggtgtt cctcgctccg cttttcccgt agggaaaagt atcatggcgc
    15841 accagccgtc cggctcggca cagacagaat gtgtccgtat gccctatgat gcgccgccgt
    15901 tatcttgcgg gcatatttat caaggtgcga tcaggccacc gggaaaacac cgaaacgggg
    15961 aaagggtaag ccgtttcatg ctgccggggc cggttcgtca gttcgtgacg atagcgtgga
    16021 tgagcttgat ttccagacgg cgcttcatgg cctcgtccag acacacatgc ggatagccgt
    16081 tttcgtcata gagcgtccgg gtacacagct tgttcatgta accgctgtaa tactgcaaaa
    16141 cgcgctctac ggctccggca tccccagcgc gggcggcctc gatcaccgaa aggggcaaaa
    16201 gctcaatgcc cttgtagttc gggttcttca tccgcgtcta acctcccttc ggcatcaacg
    16261 cctttagctg tttccgcagc tccgataagg tatttgtcct gtgccgctga acggcactcc
    16321 gggacattcc cacaaggccg ccgatctcac catctgccat atccagaaca cagtgcagga
    16381 ttaaaatact ctgctccatg ctgggcagcg cggcaaaggc ttcggcaaca agctcattgt
    16441 tgatatgtag gtcatagcca tgcgacgaaa acgtaaagct gtcgctgggg tagtgatcta
    16501 ctgtacagag cttgtccaaa tctgcctgtg acaaagagct tagtgacact tcacggtcac
    16561 ggcggcgctt catttcagcc aagtagcttt tggcctcgtt acgcagtacc gtcttgcaaa
    16621 aagcatcgaa catgcaccgc gcttcatagt cgcgggggat agcatccatc ttctcacccc
    16681 ctttctgtgg gggacttagg tggttctctc cctttccgct aacaagactt tgacaaggcc
    16741 gccagctacc cgctaaatac ctcctttttg aaaatttctc aaaaaatttt tttggggaca
    16801 gccgggccgc aaaagacgac aaaaatcgcc cggcaggtcg caaacctacc gggcgagaaa
    16861 atgctttgct atgctgctga tttacatggt atagtacata ctcataaccg gaatagccct
    16921 gtg
//
LOCUS       DAYYKN010000057        16349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000057 DAYYKN010000000
VERSION     DAYYKN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.057"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..1593)
                     /gene="guaB"
                     /locus_tag="ACHBB7_001564"
     CDS             complement(109..1593)
                     /gene="guaB"
                     /locus_tag="ACHBB7_001564"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM9209521.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(2098..2793)
                     /locus_tag="ACHBB7_001565"
     CDS             complement(2098..2793)
                     /locus_tag="ACHBB7_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM9209522.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(2883..3983)
                     /gene="ychF"
                     /locus_tag="ACHBB7_001566"
     CDS             complement(2883..3983)
                     /gene="ychF"
                     /locus_tag="ACHBB7_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM9209523.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(3992..4183)
                     /locus_tag="ACHBB7_001567"
     CDS             complement(3992..4183)
                     /locus_tag="ACHBB7_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM9209524.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(4204..5094)
                     /locus_tag="ACHBB7_001568"
     CDS             complement(4204..5094)
                     /locus_tag="ACHBB7_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM9209525.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(5081..5848)
                     /locus_tag="ACHBB7_001569"
     CDS             complement(5081..5848)
                     /locus_tag="ACHBB7_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM9209526.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(5860..6573)
                     /gene="rsmG"
                     /locus_tag="ACHBB7_001570"
     CDS             complement(5860..6573)
                     /gene="rsmG"
                     /locus_tag="ACHBB7_001570"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM9209527.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(6805..8706)
                     /gene="mnmG"
                     /locus_tag="ACHBB7_001571"
     CDS             complement(6805..8706)
                     /gene="mnmG"
                     /locus_tag="ACHBB7_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM9209528.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(8723..10120)
                     /gene="mnmE"
                     /locus_tag="ACHBB7_001572"
     CDS             complement(8723..10120)
                     /gene="mnmE"
                     /locus_tag="ACHBB7_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM9209529.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            10419..10802
                     /locus_tag="ACHBB7_001573"
     CDS             10419..10802
                     /locus_tag="ACHBB7_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209530.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(10970..11728)
                     /gene="jag"
                     /locus_tag="ACHBB7_001574"
     CDS             complement(10970..11728)
                     /gene="jag"
                     /locus_tag="ACHBB7_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM9209531.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(11746..12564)
                     /locus_tag="ACHBB7_001575"
     CDS             complement(11746..12564)
                     /locus_tag="ACHBB7_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM9209532.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(12561..12917)
                     /gene="rnpA"
                     /locus_tag="ACHBB7_001576"
     CDS             complement(12561..12917)
                     /gene="rnpA"
                     /locus_tag="ACHBB7_001576"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM9209533.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(13035..13169)
                     /gene="rpmH"
                     /locus_tag="ACHBB7_001577"
     CDS             complement(13035..13169)
                     /gene="rpmH"
                     /locus_tag="ACHBB7_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM9209534.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            13693..15027
                     /gene="dnaA"
                     /locus_tag="ACHBB7_001578"
     CDS             13693..15027
                     /gene="dnaA"
                     /locus_tag="ACHBB7_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM9209535.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            15225..>16349
                     /gene="dnaN"
                     /locus_tag="ACHBB7_001579"
     CDS             15225..>16349
                     /gene="dnaN"
                     /locus_tag="ACHBB7_001579"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM9209536.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRT"
BASE COUNT         4479 a         3478 c         2932 g         5460 t
ORIGIN      
        1 gaatttcttc ttatttccga aggagttgct tctacttccg ccgtttatac gtgtttagag
       61 cgtgagacaa aagtgttctt tacttttgtc ccacgctctt taatgttttt aagattcaac
      121 tgaatagttt ggtgcttctt ttgtgatttg tacatcatga gggtgtgatt cttttaatcc
      181 actgccgctc atttggataa attgcgcttc atcacgtaat tgctgtagat tggctgcccc
      241 aacataaccc attcctgaac gcaaaccgcc gatcatttgg aaaataatat ctgcaacact
      301 tcctttataa gcaacccgac cttcgattcc ttctggtact aatttatttg cttcattgac
      361 actaccttgg aagtaacggt cttttgaacc tttttccatc gcgcctaatg agcccatacc
      421 tcggtatgtt ttgaatcgac gtccttggaa gatttcgaat tcacctggtg attcatctgt
      481 ccctgccaac atacttccta gcatcactgc atgtccgccg gcagctaatg ctttgacgat
      541 gtcaccagaa tatttgatcc ctccgtctgc aataatcgct ttgccatatt gacgagcaac
      601 tgatgctgca tcataaatag ctgtgagctg aggtacacct acaccggcta ccacacgtgt
      661 cgtacaaatc gatcctggtc cgataccaac ttttacgaca tctacacctg catcatataa
      721 agcttttgtc gcttcagccg tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt
      781 tgaacggatt tcttgaattt ttcggatcac acctgcacta tgtccatgag ctgtatcgat
      841 gatgattgca tctgcccctg cttctaataa agcattcgca cgctcaaatg tatcgcttgt
      901 tacaccaacg gctgctgcta caagcaatcg accatgttca tcttttgctg catttggaaa
      961 ttcgatcact ttctcgatgt ctttgatcgt gatcaatccg cttaaacgac cttcgttatc
     1021 tacgattggt aatttttcga ttttatgttt ttgcaaaatc ttttctgcat cttttagaga
     1081 ggtacctact ggggcagtca ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg
     1141 ataatctgtt acaaaacgca tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc
     1201 aagtgtttca acgattggca caccactgat acgatatttg ctcatcaatt cttctgcgtc
     1261 tgctacaaga tgctgcggcg ttaagaagaa cggatcaatg attacgccac tttcagaacg
     1321 tttgactttt cttacttcat ccgcttgctg gctgatcgtc atgtttttat ggatcacacc
     1381 tagtccgcct tgtcttgcca tagcaatggc catcttgctg tcggtaacag tatccatact
     1441 tgcactgatg attggaatat tcaacttaat atttttagct aattgtacgc tcatatccac
     1501 ttcgtttggt aacacgtgac tttctgctgg tatcaataag acatcatcaa aagtaaatcc
     1561 ttttttcgtg aactttgttt cccagttgga catttttagt accactcctc taatttagtt
     1621 tatctattaa aataattgat tttttcattt aagtcaatga tctacttgga atcaagttag
     1681 ctttctcata gaataccaat ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa
     1741 aaaagctgtc ctgcatcaag taataagaac agccaatcaa aaacagcttc tcatgttttt
     1801 gaattggttg ggtttattac cgcagatgca aacttttgaa caccgtttag tggagaggct
     1861 gtgaaaaagc tgcttagttt cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat
     1921 ttttgcataa attggactta ataccgagaa actggctttt gaacaccgtt tatcgtaaaa
     1981 agggactgtg acaagacttt tgtcacagtc ccttactatt atttaactat ggataaacaa
     2041 aatgaggttc tttactaccc ctagatatct agcaaaatag ctgatctttt tgctagttta
     2101 tcttcttggg acggataaac ttccaataat cccgtatttt ttcttcaagt agtaacggat
     2161 tgctaaagca gttgccccga taatgataac tactactgga tctaatactg gattgataac
     2221 tggtggcaat agtgttgtcg cagaaaacag tacgacccaa agaagtgtcg ttccacctag
     2281 gataagcaat gtcttaccca ttcctggttt cttgcttcga tctgctccgg gatattcata
     2341 ttgatagatg tatttgtaca tcatataaaa gacccagcca ccgattgcag aacctagaac
     2401 gagtgtagaa aagccataag ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat
     2461 catgatactt aatagaccaa gcaacagcaa cgtattatct aaccacatca ataaaggggt
     2521 ggatgaattt tttacttctg ctggcttatt gatgattgct tccgttcttt cagagactgt
     2581 cccaaacaat tgacgggctg ttcttccgcc tttttgttct gcaactaaag taggcagaat
     2641 gtcatgaagg gcttttactt tttcttcttc agacaaattc gctgcttcta aagatttttt
     2701 caaatcaaaa atgtactgtt gatttctttt ggttaatttt gtttctaaac tattgttttc
     2761 ttttacgatt gctcgtaatt gttctggttc cattatctaa tagtcctcct tagccgttcc
     2821 ttttctcaac ggtcgtcaca aaagacattc taccatattc tacagctagt ttgatagccc
     2881 ttttacacat taaagcggaa taacattacg tcgccgtctt gtacgacata atcttttcct
     2941 tctaatcgaa cacgtccagc ttcttttgct gcatgcatgt ttccgtaatg atccaagtct
     3001 tcaaaagaga cagtttctgc acggataaat ccgcgttcga aatctgaatg gatgattcct
     3061 gctgcttgtg gtgctttgat tccttttttg aatgtccaag cacgtacttc ttgttcccca
     3121 gcagtaaagt aagtagctaa gcctaacaaa tcataagcag cacggatcaa ctgatccaaa
     3181 cctgattctt cgattcctaa agcttctaaa aattccgctt tgtcttcttc atctaattct
     3241 gcgatttctt cttctgcacg agcactgacc acgatcactt ctgcattttc ttctgcagca
     3301 aagttgcgca cttcttgtac ataatgattc gcgtctgcat ctgctacgtc atcttctgag
     3361 acattggcta cataaagaat tggttttgtc gtcaataaaa atagtgattt aacgattttt
     3421 tgttcttctt cagtaaattc gatcgttcgg gcagattttc cttcttccag tacaggtttg
     3481 attttatcca gtacagctag ttctgccact gcatctttat ctttggtttt agcaactttt
     3541 gctacacgag tatagcgttt gttgatagat tctaagtctg ctaacactag ttccaagttg
     3601 atcgtatcaa tatcttctag tggatcaaca cgtccttcta cgtgagtaat attatcatca
     3661 tcaaaacagc gaacaacgtg acaaatagcg tctacttggc ggatatggct taagaattga
     3721 tttcccaaac cttctccttt acttgcgcct tttacgattc cagcaatatc tgtaaattca
     3781 aaagtcgtcg gaacagtttt tttcggatgt acaagttctg ttaagcgttg caaacgccag
     3841 tcaggtactt ctaccatccc tacgttgggg tcgatcgtcg caaagggata gtttgctgct
     3901 tctgctcctg cttttgtgat cgcattaaac aaagttgatt ttcctacgtt gggcaaccca
     3961 acaattccag cagtcaatgc cataaattta ttcactcttt ctctgcattt ttttcaatta
     4021 ctttttttaa tttcttttca aaatctcgac gggtcatcat gacgatatgt ccgcaattca
     4081 tacatctgat tttgatatct gcgcccattc gaatgacttc ccagcgattc gtctggcaag
     4141 cgtgaggttt tttcatttca acgatatcgt gcaagtcgta cataaaaata gcctcctttt
     4201 tgtttattct tcatcaaatt gaatattcaa gatatcaaga attcgagtca aatctttttg
     4261 agaaagatat tcaatctcga tcttaccttt cccatttttt tcttggatag ctacacttgt
     4321 accaaattta tccatcaaac gatcttcgct ttctcggata taatatggtt tttcctttgc
     4381 tagccttggg atttttttct tttcttttcc atcattttga tttaactcag aaacaagctg
     4441 ttccagttgg cggacagtta aattttcagt tactgcacga ttggctaatt tcaagatcaa
     4501 tgttttattt tttaaaccta acagcgtacg ggcttgtccc atagataaac gctgatcttg
     4561 taccatttcc ttgacaagat ccggtaaaga aagcaagcgc aaataattgg cgatataagg
     4621 tctgctcttt cctaaacgac tagctacttc tgcttgtgtt agtttcaggt ttttcatcag
     4681 catctcatat gcttctgctt cttctaacgg attcagatct tctcgctgca agttttccag
     4741 cactgctact tgcatcatcg cttcttcgtc aaagtctcta acgatagcag ggatcgtttc
     4801 tttttcagct agtttggacg ctcgaaaacg tcgctcccct gcaataatct catatccttt
     4861 tactgcggaa cggcgtacga tgatgggttg aaagacacct gattgttcaa tagaattagc
     4921 tagttcctgc aaagatactt cttcaaatgt tttacggggc tgatacggat ttggtcgcag
     4981 ctcattcaat ggtagctgga caacggtatc atcttttaca tccaccgtct ctaagttttc
     5041 taaatcttga aacagtgcat cgattcctcg gcctaatcct ttacttttct tcacgactta
     5101 ccacttcctt tgccaatgct tggtagactt cagctcctcg agaacgtgga tcgtagtcga
     5161 tgatcggttt tccatgacta ggtgcttctg ataaacgaat atttcttgga atgatcgttt
     5221 cgtaaacttt ttcgcggaag tatttacgta cttcttctac gacttcattt cccagatttg
     5281 ttcgtgcatc atacattgtc agtaagactc cctcgatctc taattctgga ttaaagtgtt
     5341 tttggactaa acgaaccgta ttcagtaatt ggcttaatcc ttccaatgcg taatactcac
     5401 actgaacagg tatcaaaatc gaatcacttg ctgtaaatga attgattgtc agatggccaa
     5461 gagaaggcgg gcaatcgatc aaaatataat catactgact gcttacttct gctaaagatc
     5521 cttttaatct tgattctctt gccatcattg acgtcaattc gatctctgct cctgctaatt
     5581 ggagagtcgc aggcacaata ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa
     5641 ttggcaattc attgactaga acatcataga tgtctcttgt cacatcaggt tttctgatcc
     5701 cgacaccact ggtagcattt ccttgtgcat ccatatccac tagcaatact tttttgccaa
     5761 ggctagcaag acaagcacct agattcaccg tcgtcgttgt tttgccgacg ccaccttttt
     5821 ggttcgcaac agatattatt tgtgccatct cttctcctcc tatttgattg gctgcttatt
     5881 aggcagtccg ggctttcttg ggtatttctt tggggttggt ttcttttttt gaataacgat
     5941 gatatgccgt tgatcagaga cattcggcaa ggtaaccgcc tcttctttga ggaatttacc
     6001 acctaataaa gcaatcgctt gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt
     6061 caatgcataa aaatagcctt cttgtttagc caagggcaaa cacaattccg ctaacacatt
     6121 caaacgagca accgcacgag cagtgacaag gtcaaatgat gcacgaaagt gaggattttg
     6181 cccaaatgtt tctgcccggt catgatataa ggaagcatcg attcctaata atcgggttaa
     6241 ctcagataag aaggttattc gtttattcag agaatcaacg atcgttatct gcagttctgg
     6301 aaaggcaatt tttaaaggta tgctaggaaa ccctgccccc gaaccaacat cgcataaaga
     6361 taaaggttgc gtaaagtcta cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag
     6421 atatacgtct ttttcatccg taatagctgt aaggttcatc ttttcgttcc attcgactaa
     6481 tgtccgataa taaatagcga attgattcat ttgatgatcg gtaagctcta tccctttttc
     6541 tgccaaggat tggcgaaatt cttctggtat cataaatatt cctttcattg tgaaacagca
     6601 attgtttcac agattctctc cctactttaa cgcaaaatct ctagacatgc aatccttcca
     6661 tatggattct taaaaatttg aaaagtaaga aaagccgata gactttttct ttctagtatg
     6721 actcataaaa tatacacaga agagagaact agctttgagc aatttctgca aaaactagtt
     6781 ctctcttcta tttattatta cgtattacgc tggttcactg actttcgcaa tcttcccttg
     6841 ttcgatatag accattagga tacttaggtc tgcagggttt acgccactga ttcggctagc
     6901 ttgggcaatt gtttctggtt ggattttctg caatttttgt tttgcttctg ttgctaatcc
     6961 gttgattgct gcgtagtcga tgttttctgg aatctttttc gcttccatgc gttttaattt
     7021 ttctactttt tccatggctt ttttgatgta gccttcatat ttgatttgga tttctacttg
     7081 ttcgatttct tttcgtgtca attcttcatt ttctggaata aaccgtagaa gttctgcata
     7141 agtaatctca ggacgtctta aaaagtcgct tgctagtaca ccatctttta atggtgccgc
     7201 attaatcgtt gctaaaaagt cttggacttc ttctgttggt ttgattcgta cattcatcaa
     7261 acgtttgatt tcttgttcaa ctgcttcttt tttcttcaaa taattcttgt attgatcttc
     7321 ttttactaag cctacacggt gacccatttc tgttaatcgc aaatctgcat tatcatgacg
     7381 taaaagcaga cggtattcag cacgtgaagt cagcaaacga taaggttcgt tcgttccttt
     7441 tgttaccaaa tcatcgatca tgacaccgat gtaaccgtca cttcgtttga tcactaaagg
     7501 ttcttttccc tgaactttca aagctgcatt gataccggca atcagccctt gtccagccgc
     7561 ttcttcataa ccgcttgtac catttgtttg tccagctgta taaagatttt caacgacttt
     7621 agtttctaat gtcggacgga gctggtgtgg ctctactacg tcatattcga tagcatatcc
     7681 tgtacgcatc atttctactt tttctaatcc agcaatagaa tgaagcatgt tgacttgaac
     7741 atcctctggt aaagaagtag ataaaccttg aacatagact tcttccgtat ttaagccttc
     7801 tggttccaaa aataattgat gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat
     7861 ggatggacaa tagcgagcgc ctaccccttc aacgatccct gtaaacattg gggcacgatg
     7921 taagttgtct cgaataattt ggtgagtcgt ctcacctgta taggtcagcc agcaagattc
     7981 ctgatctagt ttataggcac tatctggtgt gctgaagcta aaatgattcg gctctttgtc
     8041 accaggctgc tcttctgtaa cagaatagtc gatcgtactt gattttactc gaggcggtgt
     8101 tcctgttttg aaccgttcga tagcaaagcc aagttctttt agatgattcg ctagtccaat
     8161 agaaggttga gagttattcg gacctgatga atatttcaat tcaccgataa tgatttctcc
     8221 gcgaagtgct gttccggctg taataacaac tgctttggcc cggtagctcg ctcctgtgga
     8281 cgtcactact ccatgacaaa caccatcttc gacgatcagt tcttcgacga ttccttgtct
     8341 tagcgtcaaa tttggttctt tttcgatcgt atgtttcatt tctgttgaat aagcatgttt
     8401 gtctgcttgt gctcgcaatg ctcggacagc tgggcctttt cctgtgttca acatacgcat
     8461 ttggatatag gttttatcga tattcttacc catttctccg cctaacgcat cgatttctcg
     8521 aacgacaacg cctttagctg gtccgcctac agaaggatta catggcatga atgctaccat
     8581 gtccaaattt attgtcagta ataatgtttt tgatcccata cgtgcagcag caagtgcagc
     8641 ttctgaaccg gcgtgtcctg cgccaacaac gatcacgtca tacgcatctg cttcataatg
     8701 ttccaatcgt ttgttcctcc ttttattttc ctaaacaaaa ttgactgaat aattgagtga
     8761 ttagctcatc ttgtacactg tcgccgacaa cttctcctag atagtcccag caacgcgtca
     8821 tatctatttg aacgagatcc acaggcattc ctgaatcaat acctgcaatg acttcttcaa
     8881 gtgagaggga agctttttct aacaacgcga tatggcgggt attagaaaca taagtagcgt
     8941 ctctttctcc tgtttctccg ctgaagaata aatcagaaat agctgattcc aaacgatcta
     9001 gtccgtcgtt tttagctacg gaaattgaaa aaactggttc attttcgatc aatttcttca
     9061 atttttcttg ttccagctgt gctggcaaat ctgttttatt tagaagaatg atccgtttca
     9121 atccacttgt cgcttccaat aattgttcat cttctgctgt cagtggttcg ctttggttta
     9181 gaacaagtaa gatcaagtct gcttctgcta atgcttttcg gcttctttcg accccgatcc
     9241 gttccaccac atcttctgtt tcccgaattc cagctgtatc gattagcttg agcggtaccc
     9301 cccgaacatt gacatactct tcgattacat ctcgagtcgt tcctgcaata tccgtaacga
     9361 ttgctttttc ttctcttaac aaatggttta acaaacttga tttgccgaca tttggccggc
     9421 cgatgattgc cgtacttaac ccttcccgca gaaccttccc ttgcttggat gtagcaagta
     9481 gcgcttggat tctttgttgg atcatctgcg ctttttcaag caataacttc gtagtcaatt
     9541 cttcgacatc atcatattct ggataatcaa tatttacttc tacttgtgct aatgtctcta
     9601 aaatctcttg tctcaatgag cggatcaaag cagacagatt gccgtctaac tgattaagag
     9661 ctagtcccat tgctttgtct gtttttgccc gaatcaaatc catcacagct tctgcctgtg
     9721 aaaggtcaac acgaccattc aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc
     9781 ttgcaccttc tcttaacaat agctgcaaaa tttgattgac tacaacgatc ccaccgtgac
     9841 aattgatttc tactacatct tcccgcgtaa atgtctttgg tgctctcatc acggagacca
     9901 tgacttcatc gacaagctga ttgctcttgg gatcaacgat atgtccataa tggatcgtat
     9961 gacttggtac ttctgataat cgtttgtttc cagattgata aactttatct gctagttgca
    10021 cagcttgatc gccacttaaa cgaacgatac tgattgcacc ttctccaggt ggggtagaaa
    10081 ttgccgcaat cgtatcaaat tctaaagtaa tttcagccat tttttcactt cctttttcat
    10141 tttgcgcaca aaaaaagtgc ccactccacc ctttatgcac aataaacggt gaacttcgca
    10201 ctttgactgc ttgttcttga ttaaattctg gtcatagatt atcacacttg ctataaggaa
    10261 tcaagtgttt aaaacaatta gatgaagttg agcctgttct gcttccatta ccttatattt
    10321 aattgtcctt ggatgtctct ttgcgttctt tggtttattc gaagagaaga tactgtataa
    10381 acaaggcttt atctttctta aaaaaaacgg tacactaaat ggtagcgcta tctaaactat
    10441 acacttttga aaggagaaaa tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa
    10501 gtgaccctcg gatcaacgat gtcgttttat ctacagggaa tatcaaccag aaatatatcg
    10561 tccgcgatat cgtttttgtt gtggaatgct tagaagctga tttgtttgac ccatcttttc
    10621 ccaaagatga ccttttctta acaacgaaga ggaaattaaa gacgaaggta cttgaatatg
    10681 gtgcgaatgc agtaatcaac tgtcattttg agtatcagca aaaaaaacgc gacggaaagt
    10741 attttctgga actttttgct tatggaacag tgatccaatt tatccaaaca acgattggtt
    10801 aaatgaaaca gacaaaaaaa gcggatcaaa actgaagaca gtttcgatcc gcttttttct
    10861 atcaaagtta gtgactactt catcgtatac tctgataaga atgatttcta gtaaaatata
    10921 cttggtttga aaaactttgt caacgtgatt tattcaaaaa gtaatggaat caataatatt
    10981 ttttagcagg ttctactact aaataacgat aaggttcctc accttccgaa tgtgttttta
    11041 catgttcatc tttgcttaat gcagcatgga tttgtttacg ttcaaatgca ggcattggtt
    11101 ctaagaaaac aggctgccct gtccgtttta ctttttcagc cgtgcgatca gctaaacgct
    11161 gaatgatctc ttggcgcttt tcacggtaat tgcctacatt gactactaca gacagcttat
    11221 ttcttgctaa acgatggatg aagacttgtg ccagatattg caatgcattc agtgtttttc
    11281 catgtttccc aatcagtatg ccttgctttt gtgtttctaa atggaaaaca accatattat
    11341 ttgattctct ttccattttg acaagagctg gagcatcaag ctgttttgaa atttcagtaa
    11401 gataaactgc caattccgtt aaagctgctt catcacctag ctcacttact tcttctgttt
    11461 tttcttcaag aatcgtttcg ttgactacct cgatctcagc tgttggttct tcttccaaaa
    11521 tttcttctac agtctgttct actgtttcag aaactttttc gctgatactt ggctggatcg
    11581 aaagccgtgc attctttcga cccattccta ggaaaccttt ttttccttct tccagtattt
    11641 cgatttcgac ttggtctttg gccaaaccca aatcagccag acctagttgg acagcttcgt
    11701 ctattgttgc agcttcataa atcggcatta gcattacctc ccctattatt tccgtttttt
    11761 cttaggattc attgctttct taagagcacg ttctctttcg cggattctcc gtgcttcttc
    11821 ttcacgctct tttcgaattt tgaaagggtt gttgatcagc agcgtttgta caacttggaa
    11881 ggcattggaa actacccagt ataatgaaag accgctggct aaattgacac ccatcactaa
    11941 aatcattaca ggcatcacga aattcatgat cttcattgat gcattcgatt caatttgact
    12001 catgctagat aaatacgtac ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa
    12061 tgttggatcc gcgttcccta agttcaacca taagaaatgc ccttgtttca actctggtac
    12121 acgtgaaata gattgccata acgccatcaa gattggcatt tgtactaata aaggaagaca
    12181 accagcatat ggattgacgc cattttctgc atataaacgc tgctgctctt ctcttaactt
    12241 actttgtgtt tccggatctc ttgaactata ttgttgctgc agtgctttta atttcggttg
    12301 taattcttgc gttttacgca tgctctttgt ttggaaatgc atcaacggca ataggatcac
    12361 gcggatgatc agtgtaaata aaatgatccc tatccctgca ttaccaaatg acaacgcctt
    12421 gatcgcctca gcaaagtaat aaacaatata acgatcccaa attcctgtac tctgtgcgct
    12481 gacttcccca gttccgcaac cggataagac gatgaccaaa ctaacgatac cggccatcaa
    12541 aagtaaacgt ttgtatttct tcacttactc ttttcctctc tgtcgattat ttttgcaagt
    12601 tttaagacgt ggactaaatt ggagcgaacc tcatcataag tcaacccctt cacacttggt
    12661 ctagcaatca caataaaatc caactccgga tcaatttctt cttttataga ataaactgct
    12721 gctcggattt ttcgtttgac ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca
    12781 gataaaccaa ctcgaaaatg aggctgtccc ggtttttcca aacggtaaac gacgaatttg
    12841 cggttagcaa aagaatctcc cgtatggaac acggcttgaa actctcgttc tttttttact
    12901 cgatacgctt ttctcattag ttttctttcc ttttctatct ctcacttacg ctcataatca
    12961 taccataatt acgattgtcc tttcatcatt tccctaaaaa agagtataaa aaaaaccact
    13021 gaaaccccag tggcctaagc tgaaagaact tttcttcctt tgcgacgacg gctagctaac
    13081 acacgacgtc catttttagt actcatacgt ttacggaatc cgtgaacttt ttgacgttta
    13141 cgtttatttg gttggtatgt tcttttcata tattccacct ccaaaatgtt cttatctatt
    13201 ttataaacac agacatactt tgatagtata acgacaaagt agtgattttg tcaatataaa
    13261 atagaagatc gattgttaaa acttatccac aagattattt ttttctatgg gagtcctatg
    13321 tggaaaaagt tatgcacagg gattatccac agataataaa tggctggttt tacacagaaa
    13381 acacacgtgt aaaaaaagta atccacaatc aggtgatttc ctgtgtattc cgtctcctaa
    13441 ctattggtat atctatattt tttatccaca gatgcaagag gaaatctatt gatttttacg
    13501 agttttccac ctgtgcatta gtggtgtgga aaactttttt ttgacaactg tgtttttctc
    13561 ttttttcttt ttgtcgattg tgtggataat tgtaattaaa aaattttttt cttcggtttc
    13621 tgtggaaaac tattgaaaaa aatgctaaat tagtactagt tattcacttt ttaatgaagg
    13681 aggaggcctt atatggtatc cctcgatgct ttatggaatg aattaaaagc aacataccaa
    13741 aaggatttat cgcctgctag ctataataca tggatcgaaa cagcgaaccc tcgaaccctt
    13801 gatcaaaacc agctcgttgt tgaggttcct agcaagattc ataaagaata ttgggaaaaa
    13861 aacctggcga caaaaatcgt tgaagtcggt tatatgttat caggaaatga gattatccct
    13921 cgctttatta ctggcgaaga agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa
    13981 gttgtggcac ctagtccgct aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg
    14041 tttgtcattg gtaaaggaaa ccagatggct catgcagcag cgctcgttgt agcagaagat
    14101 ccaggttcta tttataaccc gctcttcttt tatggaggcg tgggacttgg taagactcac
    14161 ttgatgcatg caattggtca tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat
    14221 gtaagcagtg aaacttttgc taatgatttc atcaattcga tccaaaacaa aacagcagaa
    14281 gagttccgtc aagaatatcg aaatgttgat ttactgctag tggatgacat tcaatttttt
    14341 gcagaaaaag aagctacgca agaagagttt ttccatactt tcaatgcctt gtacaatgaa
    14401 gggaaacaaa tcgtactaac cagtgatcgg ttgccaaacg agattccgaa actgcaagaa
    14461 cgcctcgttt ctcgctttgc atgggggctg tctgttgata ttacaccgcc ggatcttgaa
    14521 acaagaacag cgattttacg taaaaaagcg gcggctgaac gtctagagat cccagatgac
    14581 acactaagct acatcgcagg acaaattgat tctaacattc gtgaattaga aggtgcgctt
    14641 gttcgagtgc aagcttttgc tgcgatgaat agtgaagata tttcgactag tctagctgca
    14701 gacgcactga aaacgttaaa atcaggaaaa ggacatcctc aattgtcaat cctgcagatc
    14761 caagaagaag tggcaaagta ttatcacatc caattgaaag atatcaaagg aaagaaacgc
    14821 gtcaaatcta ttgttgttcc tagacaaatc gctatgtatt tggcacgtga gctcacagat
    14881 aattctttac cgaaaatcgg agctgaattc ggtggcaagg atcatacaac ggttatccat
    14941 gctcatgaaa aaatccagca gctgatggat tctagtgtca gtatgcaaaa tgaagtatca
    15001 gaaatcaaaa actttttact aaattaattt gtcgattcta tcaactgaaa atagcatgtg
    15061 gataaaagaa aaaaacgttc aaaagttatg cacacttttt ccacagctgt tttttcgttt
    15121 ctctcttacg ttttttgagt tttccacaga atcaacatgc cctattactt ttattattct
    15181 ttttaactaa taataaataa acaaacattc aaaggagata catcatgaaa gttactttaa
    15241 accgagctag ctttatgcag gaattgcaaa ctgttcaacg agctatttca agcaaaacca
    15301 cgatccctat tttgacaggt gtaaaaatca cactgacaca agaaggtttg actttgacgg
    15361 ggagcaacgc tgatatatca attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata
    15421 tgcaaatcga atctactggt tccattgttt tacaagcacg tttctttagc gaaatcattc
    15481 ggagacttcc tgaagaaaca tttactttag aagttttaga aaataaacaa gtagcgatta
    15541 cttctggaaa agcgaatttt atcgtaaatg gattagatgc agataactat cctcatcttc
    15601 ctgttgtcga aagccataac cagatgaaat tacctgtaca cgtattgact aaactaatca
    15661 acgaaacagt ttttgctgtc tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt
    15721 tcattttatc tgataattct ttattggctg tagctactga ttctcaccgt ctaagtcaac
    15781 gcgtgattcc agtagaacaa gcggctgatc attttgatat tgttattcct ggaaaaagtt
    15841 tgatcgaatt atctcgctca ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg
    15901 aaaaccaagt gttgttcaaa acagaaacga tgtatttcta ttcccgtttg ctagaaggaa
    15961 actatcctga taccaatcgt ttgattcctt caagttttaa tacagaggtt gaattttctg
    16021 ttcctagctt tttagcggcg atcgaacggg cttctttact ttctcatgaa ggccgtaaca
    16081 acatcgttcg tttatctatt cgtccagatg cggttgtttt atacggaaat tcgccagaaa
    16141 tcgggaaagt agaagaaagc ttgagttata cagcaagcag tggcgatcca ttagatattt
    16201 ctttcaatcc ggattatatg aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag
    16261 tgaaatttat ctctgctatt cgtccattta cattggaacc aacggaagat ggcgttcaat
    16321 ttattcagct gattactcct gtacgtaca
//
LOCUS       DAYYKN010000058        16071 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000058 DAYYKN010000000
VERSION     DAYYKN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.058"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..870
                     /locus_tag="ACHBB7_001580"
     CDS             13..870
                     /locus_tag="ACHBB7_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM9209537.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            985..1905
                     /gene="pstC"
                     /locus_tag="ACHBB7_001581"
     CDS             985..1905
                     /gene="pstC"
                     /locus_tag="ACHBB7_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM9209538.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1905..2789
                     /gene="pstA"
                     /locus_tag="ACHBB7_001582"
     CDS             1905..2789
                     /gene="pstA"
                     /locus_tag="ACHBB7_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM9209539.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2804..3610
                     /gene="pstB"
                     /locus_tag="ACHBB7_001583"
     CDS             2804..3610
                     /gene="pstB"
                     /locus_tag="ACHBB7_001583"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9209540.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3626..4384
                     /gene="pstB"
                     /locus_tag="ACHBB7_001584"
     CDS             3626..4384
                     /gene="pstB"
                     /locus_tag="ACHBB7_001584"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9209541.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4397..5074
                     /gene="phoU"
                     /locus_tag="ACHBB7_001585"
     CDS             4397..5074
                     /gene="phoU"
                     /locus_tag="ACHBB7_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM9209542.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5312..5611
                     /locus_tag="ACHBB7_001586"
     CDS             5312..5611
                     /locus_tag="ACHBB7_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209543.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5652..6344
                     /locus_tag="ACHBB7_001587"
     CDS             5652..6344
                     /locus_tag="ACHBB7_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM9209544.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6341..7108
                     /locus_tag="ACHBB7_001588"
     CDS             6341..7108
                     /locus_tag="ACHBB7_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM9209545.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7208..8776
                     /gene="liaX"
                     /locus_tag="ACHBB7_001589"
     CDS             7208..8776
                     /gene="liaX"
                     /locus_tag="ACHBB7_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM9209546.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9073..9393
                     /locus_tag="ACHBB7_001590"
     CDS             9073..9393
                     /locus_tag="ACHBB7_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM9209547.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9393..9743
                     /locus_tag="ACHBB7_001591"
     CDS             9393..9743
                     /locus_tag="ACHBB7_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM9209548.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9910..10848
                     /gene="hprK"
                     /locus_tag="ACHBB7_001592"
     CDS             9910..10848
                     /gene="hprK"
                     /locus_tag="ACHBB7_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM9209549.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10863..11693
                     /gene="lgt"
                     /locus_tag="ACHBB7_001593"
     CDS             10863..11693
                     /gene="lgt"
                     /locus_tag="ACHBB7_001593"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM9209550.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11737..12762
                     /locus_tag="ACHBB7_001594"
     CDS             11737..12762
                     /locus_tag="ACHBB7_001594"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9209551.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12777..13715
                     /gene="galU"
                     /locus_tag="ACHBB7_001595"
     CDS             12777..13715
                     /gene="galU"
                     /locus_tag="ACHBB7_001595"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM9209552.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13840..14280
                     /locus_tag="ACHBB7_001596"
     CDS             13840..14280
                     /locus_tag="ACHBB7_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM9209553.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14283..14885
                     /locus_tag="ACHBB7_001597"
     CDS             14283..14885
                     /locus_tag="ACHBB7_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM9209554.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14953..16059)
                     /locus_tag="ACHBB7_001598"
     CDS             complement(14953..16059)
                     /locus_tag="ACHBB7_001598"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM9209555.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
BASE COUNT         5197 a         2810 c         3493 g         4571 t
ORIGIN      
        1 aggagatttc taatgaaaaa acttaaatta ttcttaccaa taataggatt agccattctt
       61 ttatcaggct gttcaaagtg gattgatcga ggagaatcca ttacggctgt tggctcttct
      121 gctttacagc ctttagtaga aacagttgct gaaacttacc agacagaaaa tcctggaaaa
      181 ttcatcaatg tacaaggggg cggaagcgga actggtttgt ctcaagtaca gtcaggagca
      241 gtcgacatcg gaaactctga tttgtttgcg gaagaaaaat cggggatcga tgcctcagca
      301 ttagtcgatc atcgagtagc agttgttggg atcactccta ttgtgaacaa ggatattggc
      361 gtaaaagaca tcagtatgga aaacctgaaa aaactattta caggcgagat caaaaactgg
      421 agtgaagtcg gtggaaaaga tcagccagta gttatattga accgcgcttc aggaagcgga
      481 acccgtagca cgtttgaaaa atgggtattg gacggagaaa cagctgttca ggcacaagaa
      541 caagattcaa gcggaatggt gcgccagatc gttgccgaca cacccggtgc aatcagctat
      601 gttgcttttt cttatgtttc cgatgatgta gatacgttga atgttaacgg tgtagaaccg
      661 acagatgaaa atgtgacaac gaacaaatgg acgatttggg catatgagca tatgtacaca
      721 agagatcatc caaaaaaact gacaaaagaa tttttagact atatcttatc tgatgaagta
      781 caagataaca tcgttggaga actaggttat atccctgttt ctcatatgaa agtcgaacgt
      841 gattggcaag gaaatatcgt taaagagtaa tctttacaca aaatttacaa acttagctaa
      901 acttttacat gttattaaaa ccaaattgat gttcaatttg tacactgagt gagtatgtta
      961 aacctgatta agggggagtc atctgtggaa gatgtgaaaa aagtattatt aacaaaatca
     1021 aaacgttcaa aaatggaaca gcgtggaaaa ttcatcagtt ttttatgtat cgcgctgatc
     1081 gtgttggttg tattatctat tttttatttt gtagctagta aaggattggc cacctttttt
     1141 actgataaga tcaatgtatt tgattttctg tttggtacag attggaatcc aagccaaatc
     1201 ggagcaaatg ggaaaccaat ggtaggggca ttgcctatga tcacaggttc gtttattgtg
     1261 acctttttat ctgctatcgt tgccacgcca tttgccattg gtgcagcagt attcatgacg
     1321 gagatctctc ctaaaaacgg aacgaagatc ttacagccgg tcatcgagct gcttgtaggt
     1381 attccttctg tcgtttatgg ttttatcggt ttatcagtta tcgtgccttt tgtcagaagt
     1441 atttttggcg ggacagggtt tggtatctta gctggtacat ttgtgttgtt cgttatgatc
     1501 ttgccgactg tcacaacgat gactgttgat gcattaaagg cagtgccacg gcactatcgg
     1561 gaagcttcat tagcgcttgg tgccacaaga tggcaaacaa tctacaaagt cgtgctacga
     1621 gcagccgtgc ccgggatttt aacagcagtc gtttttggta tggctcgtgc gtttggtgaa
     1681 gccttagcta tccaaatggt catcggtaat gcagcattga tgccaacagg acttactact
     1741 cctgcttcta cattgacgtc catcttaaca atgggaatcg gaaatacaat catgggaaca
     1801 gttgaaaata atgtgttatg gtcattagcg ctgatcttgc tattgatgtc attattcttt
     1861 aatatcgtga tccgtatgat tgggaagaaa ggagagctga aataatgaac gcaaaacgct
     1921 cagataaaat tgctacaggt gttttatata tagtttcggg gatcatcgta ttgattttag
     1981 cagctttact actttatatc ttagttcggg gaataccgca tatttcatgg tccttcttga
     2041 ccgaaccttc caaagcctac caagaaggcg gtggaatcgg gatccagcta tttaactcga
     2101 tttatttgct tttgatcacg atgatcatca gttttcctat ttctttaggt gcgggtatct
     2161 atctttcaga atatgctgga aaaaactggc taacagatgt gattcgcacg tcgattgaaa
     2221 tcttgagctc gttgccttct gtcgttgtcg gtttattcgg tttcttggtt ttcgttattc
     2281 aattccagta tggtttctcg attatttctg gtgcattggc tttgactgta ttcaacttgc
     2341 ctcttttgac tagaacagtt gaggagtctt tacaagcggt ccattacaca caacgagaag
     2401 caggattggc cttaggtttg tcacgctggg aaacagtgat aaaagtggtg gttccagaag
     2461 cgacaccagg gattttgact ggtgtgatcc taagttctgg aagaattttt ggtgaagcag
     2521 ctgcattgat ctatacggct ggacaaagtg cacctgcgtt agattttacg aactggaatc
     2581 cattaagtgt ttcaagcccg atcagcattt tccgtcaagc tgaaacatta gctgtccata
     2641 tttggaagat caatacagaa ggcacgatgc ctgatggtgt ttctgtttcg gctggcgctt
     2701 ctgccgtgtt gatcattgct gttttactgt tcaacttcgg cgctcgtgcg attggcaaac
     2761 gattataccg aaaaatgact tcagcctaag gagaagaagc aaaatgaaaa aatataactg
     2821 gaatgaaaca aatgtcatca ccatgccaaa agagaaagaa gtagcactat atacggatga
     2881 tctacatgtc tggtatggcg acaatgaagc aatcaaagga gtggatctgg aatttgaaaa
     2941 aaataagatc actgcactga ttggtccttc cggctgcgga aaatctacgt atcttcgttc
     3001 gttgaatcga atgaatgatg ggatcgctaa tacgaaaatc actgggaaaa tcatgtatca
     3061 aggcgtagat attaatgcgc ctcaagtgga tgtttatgag atgcgcaaac ggattggcat
     3121 ggtttttcaa cgtcctaatc cgttcagcaa gtcgatttat gacaatatta catttgcttt
     3181 gaaacagcac ggcgaaaaaa acaaaaagaa attagatgaa atcgttgaaa caagcttgaa
     3241 acaagctgcc ttgtgggacc aagtaaaaga cagcttggac aaaagtgcct tggctttatc
     3301 tggcgggcaa cagcaacgtt tatgtattgc tcgtgcgatc gcgatgaaac ctgatatctt
     3361 gctgctagac gagccggcaa gtgctttgga ccctatttcg acaggaacgg ttgaagaaac
     3421 acttgtaaac ttgaaagaag actatacgat catcatcgtg acgcataaca tgcaacaagc
     3481 agctcgtatc agcgactata ctgccttttt ctatctagga aaagtaatgg aatatgacaa
     3541 aactaacaag atatttacca gaccaaaaat tcaggcaacc gaagattatg tgtccggtca
     3601 ttttggttaa gacggaggcc tcaacatgac aaaagaaatt atttcatcta aagatcttca
     3661 tctgtattat ggaaagaaag aagcactaaa agggatcaat ctaagtttca atcaagggga
     3721 aatcactgcg atgatcggtc cttctggttg cgggaaatcc acttatctgc gttctttgaa
     3781 ccggatgaat gacctgattc cgaatgtaac gatcaccgga agtgttttat ataaagacaa
     3841 agacatctat ggtccaaaaa cagatgttgt tgaactaaga aaagaaatcg gcatggtatt
     3901 ccagcagcca aacccatttc ctttttctat ctatgaaaat gtcatctatg gattgaaatt
     3961 aaaaggggaa aaagataaaa aagtccttga tcaagtagtt gaagaaagcc tgaaggcagc
     4021 gtctgtttgg gatgatgtga aagacaaatt gcataaaagt gctttatctt tatccggtgg
     4081 gcagcagcag cgtgtatgta tcgcacgtgt tttggcagtt aatccggaaa tcatcttatt
     4141 ggatgaacca acaagtgcgc ttgatcctgt ttctactggg aaaatcgaaa gtatgctgtt
     4201 agaattaaga gagcggtata cgatgatcat cgtgacccat aatatgtctc aagcttcacg
     4261 tatttcagat aagacggcat tctttttaga tggtcacttg atcgaataca acgatacgaa
     4321 gaagatattc atgaatccag aaaaacaaga gacagaagat tatatttcag gtcgatttgg
     4381 ataaggagga aacggaatgc tacgtacaca atttgaagaa gaattgctga atctccataa
     4441 tcagttctac gaaatgggga tgatggtgag cagcgccgtg cacaagtcag ttcgtgcata
     4501 catcaaacac gataaaatct tagcccaaga agttatcgaa aacgatataa atatcaataa
     4561 catggaaaca cgcttggaga agaaaagttt tgaaatgatc gcattgcaac agccagttac
     4621 aactgatttg cgtatgatca ttacggtcat gaaagctagc tcggatttag aacgtatggc
     4681 cgatcatgcg gtgtctgttg ctaaatccac gattcgcgta aaagggcaga cgagaatacc
     4741 ggaaatcgaa aaagagatct ccgatatgtc cgattatgtg aaaaaaatgg tggacaacgt
     4801 cctagttgct tatgtcaaaa cagacgaaaa agacgcacgt acgatcgcca atatggatca
     4861 aagagtcaat gactatttca atcgaatcta taatgcaaca atcaaaaata tgcaggaaaa
     4921 tcctgagaca gtcatcagtg gaacagacta cttgaatgtt gccagttatc tagaacggat
     4981 cggagactat gtgacaaaca tctgtgaatg gatcgtctac ttggctacag ggaaaatcac
     5041 tgagttaaat acgaaccaca atgagatttt ctaaactgaa gctgtgatta tgccgtttgt
     5101 ttaggaaaag aggttgtgaa atcagcggtt gtgaaaaagc tgttctgcat caagtaataa
     5161 gaacagtcaa acaaaaaata gctcctcata tttttcgttt ggctggactt attaccgcag
     5221 atgcaagctt ttgaacaccg tttattcggg aaagggtttg tgacaaaaca tgtcacaaac
     5281 ccttttcatc atttcataga aaggataaag aatgcaaaaa aaaattagca tcatcactat
     5341 attttttatt tgtgtgctta cttcatgggt tattcagcat caagctgctt tatatgaagt
     5401 acctgtcatc gagatcgaaa aagtacgaat aaaagaggat catcaattga ttacaggaag
     5461 actgatgaac acggatcaaa aaggagaatc gatttattta tcggttcctt atcaaaaaaa
     5521 tcaaatagaa acagcaaata taaaagcgag acaaatgtat ctagtccaaa tgcatggaga
     5581 tgaatggcag ctagtagcaa aaaaaaaatg atggctggct tttcttcttc acaagtatct
     5641 ccattggcgc catgttgctt attggcggaa aacagggtct atttgcttta ggaagtctca
     5701 tcatcaatat cgttttactt ttacttttgt tgctcatctt tactaaaaca gaagggattt
     5761 ctctattaaa tatatctatc ctgttcgtcg ttatagggat gattattagc atattggctt
     5821 tagatggatg gaatagaagc agtataatca aaatttcagc tgcagttacg agtgtttttt
     5881 tagcattttt tatttgtctt ttgacaatga atcattggaa agaccaagga ctgcgctatg
     5941 aggaactgaa ttatctgaca cgaccgtatc gttctgtttt tctttctagc gtactgattg
     6001 gaacagctgg aggaacactc gataccgtta tcacagtcat tgcgacatta gaagaattga
     6061 ctaagcgaca accagaaata acagcgaaac agttatggct ttcaggaagg caagtgggtc
     6121 gagatgtgat tggctcgatg accaatatcc ttttgttcgt ctatatcagt gggtcagttc
     6181 catcgcttct tttatattta ggaaatcaat ggtcgttcaa agaaacgata gaacagcatt
     6241 tatcattgga atttttacgc gtcattgctg gcagcttagg aatagtatta tcgatcccta
     6301 tctcggtcct cttttttctg actgtaagga ggctgaaacg atgaatgtac ttattatact
     6361 tgttgggatt ttagcaatca gcgtgctact tattggcggt acgcaaggta tgtatattct
     6421 tttaggctta ttcatcaatt tagggatctt tttcctgttg ttattcggtt atcatcagaa
     6481 gtggccgata ctggcattgt cggtcatagg atttttattg attgctgtgg ttatcttatt
     6541 ttttatcaac ggttataatc taaaaatgcg tgcagctttt gtgagtatac tgatcttttt
     6601 attttgtttc ctactattgc ttcctatcac tgatttttta gcgatccaag gttttacatc
     6661 ggtagaacta gaagaattat ctggcttaga taagacttta gcgattgatt ttagattact
     6721 tacgagatcg ttgcttctga ttagtcttag tggtgctgtt ttagatgcta gtgtagcaat
     6781 ttctagtggg acattcgaag tatatcaggc aaatccgcaa ttatcttttt accaattgcg
     6841 ccattctagt ttcgctattg ctaaaaaaat attggcgtca acagtaatga cacttctttt
     6901 tgcattcatg ggaagttctt tagcgttgat cctctggttt attgatttag acattccttt
     6961 tcagcaagtg atcaatgaaa aatcatttgt tctagaatat acgatggcta ttcttaccac
     7021 attgtctgca ctacttgttc ttcctttaac ctgcgtgact acagcatatc tctttacaaa
     7081 aaagaaagaa caactcaaga tggagtaaaa gttacaactt aagtcctatg acatttttac
     7141 tgaatcagtg cataatatgg atgtaaggaa aagcttgcaa acaattttgc ttaaggaggc
     7201 aatttccatg aaagaaagag aacgtatttt agatttagtg aaaaaaggaa tcctttcgac
     7261 tgaagaaggc ttagatttat tagaaagcat ggcaactgaa aaagacgaaa agcaaataaa
     7321 aaaagaagca gacaaggtaa ctgctagtca taaagaaaaa gatcaggcaa gtcaattgat
     7381 cgataaactt gaaaacggcg aagaagaaat ttctgagcca gttgatccaa aggaaagaga
     7441 aaggcaagat caagaaaact tagaaaagat cttagatgaa ctagcaactg aagcgaataa
     7501 aacttcggcg cgtctagatg aagtaaatgc tcaattcgct gacaataagg cagcacgcaa
     7561 tgagaaacag gaagcattga tgcagctgaa tacaaaagaa gaattaggcg agttgacaga
     7621 agaagaatta gcacagcgtc agacgttaga agcagaaatc aaagaattag aagctagtgg
     7681 cgatacactg ttagaagaac aattgaaatt agaagcagaa ttaaaagata tccgtaagaa
     7741 tcaatggtct gagaaaaaag aaacgtttac tgataagttt gaattgccag atgattggaa
     7801 agatcaagca acagatacac tgaatcaagt aggcgaaaaa atgtcagaag ctggatcaca
     7861 gttaggcaaa ttcttgaaga aaacattcca aactgtttca gaaacagtga atgacaacat
     7921 ggaatggaaa gatgtcagct tacgtgtccc tgggattgcg acaacgaaat ttgagcatga
     7981 attttactat gaagctcctg cagcaagtat ccttgatatc aaggcagcaa acggaaacgt
     8041 gacgttaaaa acgtgggaca gcaacgatgt gaaagttgaa gctaagatca aactttacgg
     8101 aaaaatgggt gctgaaccat ttgaagcatt ctctgaacgg agccagattg aggttaatga
     8161 agatcatatc tccttccaaa ttccaaataa acgtgtgcgt gcagaccttg ttttctactt
     8221 acctaaacga gtatacgacc acgcagctat taaacttcta aatggcaaca tcatgattga
     8281 aacattggaa gctaaagata tttatacaaa atctacgaat ggtaacatca tcgtggacca
     8341 attaactgca acgatgctgg aagtagaagg tgtaaacggc aatatcgata ttcgaaacgg
     8401 gaacatctta gattctatta tcgaaacagt aaatggtaca gtgacatttg gtgcgacagc
     8461 agaaaacctt tctgtgtctt tggtaaatgg tgatgtacgt cttacgatta aagaagataa
     8521 tttgaaaaaa gtagaagcaa gttcagtgaa cggaaatgtc aaagtcgctt taccagatgg
     8581 catcggtttg gaaggacatg ctaaaacaag tcttggaagt atcaacagcc gtctgtccaa
     8641 ttacgaagta gttcgtgaga aaaaagaacg gacaaaccaa atgttgcaat tccgtcgtgt
     8701 ttcagatggt gaaatcacgc aagtgcaact ttcaacgaca acaggaagta tctatttaaa
     8761 agatacagat aaataaagag gttgtgaaag agatgttcag ccttgagcaa taagaagcga
     8821 ttttgtaaaa tagcttcccc tattttacaa aattgtgcct tattgccgat aggctatctc
     8881 ttgaacaccg tttaccaaga gattatgaac ctgccaatta tcgccgagtc acaaggaata
     8941 atttcagaaa aagctcgtca cgttttatga atatggtaat ttgtgaccga gaattttgca
     9001 gaaagacacc gattaagaag aaggttgtca caacgatttt cttcatttaa aatagtagaa
     9061 cagaggtgag acatgatgaa aaaaaagttg acaaaatcca ataaaaatgt tgttttgaca
     9121 gggactttag caggaatagg agaatacatc gggattgatc cgactgttat ccgtgtgctt
     9181 tatgtattcc tgagcttagt tgcttttgga agtccaatca ttttatacat tttattagct
     9241 ttgattatcc catctgcgcg ttctgcacga acaaactatg gacataacaa tgcatattat
     9301 gaaaaaaaca attatcgtga acaaactcgt gaacaaacaa aaaaacgcaa agaagcggaa
     9361 aaaatcgatg atgacgaatg gagtgacttt taatgtcgta ctttcaacgt attttagtta
     9421 atacgttaac attcatatca ttggctgttc tttttcctgc gatggtccat gtgagcagta
     9481 tttggacggc cgttcttgcc agcttcgtat tgtctgtgtt gaatatgttt gtcaaaccga
     9541 ttttgacgat actgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta
     9601 tcaatgcagc gattcttaaa atgacctcct tttttgtggg agcagaacat tttagttttt
     9661 cttcattcgg tgcagcgttg ttagtagcta tcttgatgtc gcttgtcaat gtaattgtca
     9721 gtgaacataa cagtagaaaa taaaagaaag ctgtgccgaa ctatcggcgc agttttttta
     9781 tttagaatca ggaatgcgtt ttctattaca ttggtaatta aataaaaacg ttttttttaa
     9841 ttggtagtac ttttggctag acaatgataa aatgatagag aacactctaa tagaaatgag
     9901 gtagcatgaa tgtcagaagt tgtaaaagtt agccaattag tgaaaaaatt atctttggaa
     9961 atcgtgacag gcgacgagaa aagcttagac cgtgtcatcg taactgggga catctcacgc
    10021 ccaggactag agttgactgg ttattttaat tattattccc ataatcgttt acagctattt
    10081 ggcagtaaag aaatcagttt tgcggaacgg atgatcccag aagagcgttt gatcatcatg
    10141 agaagattat gtagtgaaga tatgcccgca tttattattt caagaggatt accggctcca
    10201 gaagaaatga tccaggcagc aaatgaaaat gggatagctg tactgcgttc tcccatctca
    10261 acttctcgtt tattaggaga attatccagt tatttagatg gtcgccttgc ccctcgaaca
    10321 agtgtccacg gtgttttagt ggatgtttac ggattaggtg tactgattca aggtgacagt
    10381 gggataggaa aaagtgaaac agcgttggag ttgatcaaac gcggtcatcg attgatcgca
    10441 gacgatcgag tagatgttta tcagcaagat gaattaacgg tgatcggaga accgcctaaa
    10501 atcctggaac atctaattga aatccgagga atcgggatca ttgatgtaat gaatcttttt
    10561 ggtgcgagtg cggtacgtgg ctctatgcaa gtccagttgg ctgtttatct ggaagcatgg
    10621 gcaaaagata agaaatacga ccgtctgggc tctgaagaca cgaccgttga aatcgcggaa
    10681 gtgggcattc cacaaataaa aatccctgtc aaaacaggac gaaacgttgc aatcatcatt
    10741 gaagtagccg cgatgaattt ccgtgcacgt acaatgggct ttgacgcaac gaaaacattt
    10801 gaagaaagat taagtcgctt gattgaagaa aactcaggca acgactagca aggagtgaca
    10861 atatgttagc acaaatcaat cgagttgctt ttgagcttgt agggattcct atctactggt
    10921 atgcattgat catcgtatca ggtatcatta tcgccatgtg gcttagcagc agagaggctg
    10981 ttcgggtagg attaaaagca gatgatgtga cagattttat gttgtgggga ttgcctatct
    11041 ccattattgg tgctcgctta tattatgttt tatttgatct tccccagtat atagctgatc
    11101 caatccaaat attcaatatc cgttcaggag gcttagctat ttatggcgga ctgattgcag
    11161 gcgcgatcac gctgtatttt tttactaaat atcattttat ttctttgtgg acttttttag
    11221 atatcgctgc accaagtgta ttattagcac aggcaatcgg tcgttgggga aatttcatga
    11281 accacgaagc atatggacca gacacgacgc gaagcttctt agaaagtctg catttaccgc
    11341 aattcatcat tgataatatg tatatcgaag ggatctatcg tcaaccaaca tttctttatg
    11401 aatcactttg gagtttgatt ggatttattt tgcttttgtt tttacgaaag aaaccacacc
    11461 ttttgaaaaa aggcgaagtc cttttatgtt atctaatgtg gtatagtttc gggcgtttct
    11521 ttattgaagg attacgtaca gacagtcttt actttttagg tgtgatccgt atttctcaat
    11581 tattatctgc tgtgttattt gtcggtacat tgattctatt tatttggcgc agaaaaaaag
    11641 gaaatttacc ggattatgac cgctcattag gaaaaaatca acaaattatc tgatttttag
    11701 agcgttaaaa atgaaaaaaa tttcaggagt ggtaaaatga attatagagt agcggtttta
    11761 ggaccaggct cttggggtac tgctttggca caagtcctcg ccgaaaacgg acatgacgtt
    11821 cggatttggg gacatcgtgc ttcacaagta gaagaaatca acacccaaca cacgaatcaa
    11881 cgttatttac cagatcatta tctacccgat tccatccaag cattcaccaa tatggaagat
    11941 gcagtaaaag acgcagatgc ggtgctattc gtcattccaa cgaaagcaat acgttctgtt
    12001 gcaaaagaac tagtgcctaa attgaataca aagccagtga tcatccatgc aagtaaagga
    12061 ttagaacagg gaacacataa aaggatctct gacatcttgt tagaggaaat cccagcagaa
    12121 aaaagaaaag ctgttgttgt tctttctgga ccaagccatg cagaagaagt agcgaaacat
    12181 gacatcacta cgatcacagc tgcaagtaca gatgcaaaag cagcaaaata tgtacaagag
    12241 ctgttcatga ataattattt tcggatctat accaatcctg atgtgatcgg tgtagagatg
    12301 ggggcagctt tgaaaaatat tattgctatt ggtgctggcg ctattcacgg cttagggttt
    12361 ggcgataatg caaaggcagc aatcatgacg cgcggattag ctgagatcag ccgtttaggt
    12421 gtagcaatgg gtgccaaccc gctgaccttt atcggattaa gcggagtagg cgatctagtc
    12481 gttacttgca caagtgttca ttctaggaac tggcgcgcag gcaacttgtt aggacaaggg
    12541 cagcccctag aagaggtgct agccaatatg ggaatggtcg ttgaaggtgt gaatactact
    12601 caagcagcga tggaactagc ccaaataatg gaaatcgaaa tgcccatcac tcaaacaatc
    12661 tatgaagtgc tgtatgaaca aaaagaaatc aaaacagccg caaaagaaat catgttacgt
    12721 gatgggaaaa aagaaaatga attctcatta acaatttttt gaaggagcga atgtatatga
    12781 aagtcaaaaa agcagttatt ccagcggcag gattaggaac aagattttta ccagcaacaa
    12841 aagctatggc aaaggaaatg ttgccaattg ttgataaacc aacaatccag ttcatcgttg
    12901 aagaagcgtt gaaatcagga atcgaagaca tcttgatcgt tacaggaaaa gctaaacgac
    12961 caatcgaaga tcactttgat tcaaatattg aattagaaac aaacctaagt gaaaaaggaa
    13021 agaccgaatt attgaaatta gtagaagaaa cgacagatgt caacttgcat ttcatccgtc
    13081 aatctcatcc aaagggttta ggacatgccg ttcttcaagc aagagccttt attggaaatg
    13141 aaccgtttgt cgttatgcta ggcgatgata tcatggaaga tgaagtacca ttgacacagc
    13201 aattaatcga tgattacgaa cgtactcatg cttctacgat cgccgttatg caagttcctc
    13261 atgatgaaac ttcaaaatac ggaattatca atccaggaga agtattggaa gatgggttat
    13321 acaatgtcaa aaactttgtt gaaaaaccag atccaagcaa agcaccaagt gatttagcta
    13381 ttattggacg ttacttattg actccagaaa tttttgatgt attgtccgag caacaaccag
    13441 gagccggaaa tgaaatccaa ctaactgacg caatcgatac attgaataaa acacaacgcg
    13501 tatttgcccg taaattcaca ggtaaacgtt acgatgtcgg tgacaaattt ggttttatga
    13561 aaacaagtat tgaatatggt ctaacacatc cagaagtagg tgcaccttta cgtgagtata
    13621 tcctgtcttt aggtaaaaaa ttagaagcag aaggaagcaa gatcgatcat aatccaaaag
    13681 gaataacaga aatcaaagaa gaaaagaaaa aataaccctt ttttctaacg agcttcagct
    13741 gctaattgaa taaaaagtag ttgaagctcg tttttatttg tttaaaaaac acgaaaatgc
    13801 tatccttata gaaaagagag acaagaaggg agtttgatca tgtcaggagg agaaattgca
    13861 ggattgatcg cagcaatcgc ctttgcgata cttgtcgttt ttatcgttcg tgtgttgttg
    13921 caagtttcta aaactttaga aaaagtagat cagactgtag cagaagcaaa tacgacgatc
    13981 gaaatcgtca caaaagacgt agatcttttg tctagacaag tagaaggctt gctggtcaaa
    14041 agtaatgaac tgctcttgga tatcaatgga aaagtcgcaa cgatcgatcc attgttcaca
    14101 gcagttgctg atttaagtga aagtgtatca gaacttaatc attctagtaa aaacatcgcg
    14161 acgaaagtcg gcggaatcgg taaaacgaca gctaaagcga cggttgccgg aaaagtcggt
    14221 gggacagcga tgaaattttt taaaaacaaa aaacatactg aaacgacagg aggaaaataa
    14281 aaatggctaa aaaaggtgga tttttattag gtgcagtagt cggaggaaca gccgcagcaa
    14341 tcgccgcgtt attgttagca ccaaaatctg gtaaagaact tcgggatgaa ttagctgccc
    14401 aagcggatga tttgatggat atggcttcag attacacaga tatggcgaaa gaaaaatcga
    14461 ctgagttaac aggtattgca aaagaaaaag cgcaagatct ttctcaacaa gcaggtgatc
    14521 tagcaggtag tgtcaaacaa aaagtaagtg aatcagcaga tgaagcagga agtgaatctg
    14581 acgatatctt gttaaatctt aaagaacaat cagctgactt atctaatcgt ttcaaaaaaa
    14641 cggcagaaga tatgaaaaaa cctgcagatg aattaggaag tattgctaaa gatacttcag
    14701 aagatatcat gattgatgta aaagacgctg ctggtaaagc aggagatact gtcaatgaag
    14761 gaatgtcaga agcaaaacca gttgcagatg acacaaaaga aatgatgaaa atggcaaaag
    14821 aagatgcgaa agaagctaaa aaagaaataa aagatacaat gacagattca tctacaaatc
    14881 actaaaatca ctaaaatcac aaatttatag aataaaaagg tgggagcgga ctagctctca
    14941 ccttttttac tcttattttt ctaaaactag taattctttt ggtgtagtcg tgaatggcac
    15001 acagccatcg cttgtcacat atacacagtc ttcgatacgt acccctactt tttcaggaat
    15061 atagattcct ggttcaagag agaagcacat cccttcttcg attactaagt catttccttc
    15121 tacaagggaa gggaattcgt ggacggttgt tccgatcccg tgacctaaac ggtgattgaa
    15181 atattcgccg tagccatagc tgttgatcac gtttcgtgcg atttgatcaa gttcacctgc
    15241 tgtcacacct ggacggacgg cttcttgtgc ggcaagctgt gcttcaagag tgatgttgta
    15301 tattttttct tggaagtcgg ttggtttttg ataagcaatt gtacgagtag catcactgca
    15361 atagccattc caaacaacgc caagatcgaa taacacaagt tcattcggtg aaatcttcgt
    15421 gtttcctggt gtaccatgag ggcttgcagc atttgctcct gcaaggacta atgtgtcgaa
    15481 agacatttgt gaaacacctt gttttttcaa ttggtattcg atttctgcaa tgatcgcttg
    15541 ttcagtcacg ccttctttca ctgctttaaa gccaatctca aatgcaacat cggcccattt
    15601 accagcttcg atcagtttgt ctttttcctc tagagttttg atcagttgta gacgttggat
    15661 gatggaagct agatcagtgt aagaatccgt tcccgtcaag atatcagaga tagcatcgaa
    15721 gcgctcgaca gtcaaggcgt tcttttcaat ccctacattg ccaggctgac catagcgagc
    15781 acgaagttct tttccgatga tctcccaagg gttttcgctg tctaaatagc cacggacatc
    15841 atacgcccaa ctactgtttt gagcatcttc aacttccaaa gcaggagtga aaaggaaagg
    15901 atcttgttca agagggataa aaagagctag tacacgctca tgaggatcac tttcgtatcc
    15961 ggaaaagtaa gcgatgtggc cagggtcgct gatataagcc aattcgactt ggtgttgtgc
    16021 catccattga cgtagttcat tgatttttac gttattcatt tggcaaccta c
//
LOCUS       DAYYKN010000059        15801 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000059 DAYYKN010000000
VERSION     DAYYKN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.059"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..781
                     /locus_tag="ACHBB7_001599"
     CDS             29..781
                     /locus_tag="ACHBB7_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9209556.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            778..1692
                     /gene="pfkB"
                     /locus_tag="ACHBB7_001600"
     CDS             778..1692
                     /gene="pfkB"
                     /locus_tag="ACHBB7_001600"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM9209557.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1710..3641
                     /locus_tag="ACHBB7_001601"
     CDS             1710..3641
                     /locus_tag="ACHBB7_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM9209558.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3752..5311)
                     /locus_tag="ACHBB7_001602"
     CDS             complement(3752..5311)
                     /locus_tag="ACHBB7_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM9209559.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5466..7700
                     /gene="pcrA"
                     /locus_tag="ACHBB7_001603"
     CDS             5466..7700
                     /gene="pcrA"
                     /locus_tag="ACHBB7_001603"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM9209560.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7717..9753
                     /gene="ligA"
                     /locus_tag="ACHBB7_001604"
     CDS             7717..9753
                     /gene="ligA"
                     /locus_tag="ACHBB7_001604"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM9209561.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9859..10164
                     /gene="gatC"
                     /locus_tag="ACHBB7_001605"
     CDS             9859..10164
                     /gene="gatC"
                     /locus_tag="ACHBB7_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM9209562.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10164..11636
                     /gene="gatA"
                     /locus_tag="ACHBB7_001606"
     CDS             10164..11636
                     /gene="gatA"
                     /locus_tag="ACHBB7_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM9209563.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11633..13063
                     /gene="gatB"
                     /locus_tag="ACHBB7_001607"
     CDS             11633..13063
                     /gene="gatB"
                     /locus_tag="ACHBB7_001607"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM9209564.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13073..14128
                     /locus_tag="ACHBB7_001608"
     CDS             13073..14128
                     /locus_tag="ACHBB7_001608"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM9209565.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14239..15612
                     /gene="rlmD"
                     /locus_tag="ACHBB7_001609"
     CDS             14239..15612
                     /gene="rlmD"
                     /locus_tag="ACHBB7_001609"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9209566.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15615..15743
                     /locus_tag="ACHBB7_001610"
     CDS             15615..15743
                     /locus_tag="ACHBB7_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM9209567.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5373 a         2897 c         3469 g         4062 t
ORIGIN      
        1 tattatttgt attgaggtga tcgtgaaaat gcttacagag gaacgtagac aaaaaatcct
       61 agacttgctt gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc tttttaatgc
      121 ttctgaatca acgataagac gtgatttaca agagctggag aatagtgggt ttctagaaag
      181 agttcatggc ggagctaaga aagagcaatt attgaatttt gagcagaata tgagtgaaaa
      241 atcactcaaa aacgttcacg aaaaacagca aatcgccaaa tcggcagctg aaaccattaa
      301 tgacgaagat gtcatttatt tagacgctgg ctctactacg ttagaaatga ttccttttct
      361 gcaaggaaaa cagatcacag tagtgacaaa ttccgttcaa catgcggcaa atttagttga
      421 tttggagctt gctaccatta ttttaggtgg aacgataaag ctggcaacta atgctgtttt
      481 aggatctaat gcgatagaac aactaaaaaa ttttcatttt aataaagcat ttatgggaat
      541 gaacggtgcc catcttgaac aaggattcac gactccggac ccagaagagg cagctgttaa
      601 acgtttagcc atgaaaaatt ccgaagagac atttgtattg attgatcata ccaaattcaa
      661 ccagttgtcc ttcacctctg ttgcaccttt ggaagcagcc acgatcataa ccgagcgttg
      721 tccattagaa ttcgttaatg aattccgtga aaagacaaca attaaggagg caatacaatg
      781 atttacacag taacacttaa tccatcaatt gattatatcg tacgcgtaga tgggttaaaa
      841 ctaggcgatt tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa agggatcaat
      901 gtttcccgta ttttgaaacg gatcgattct caatctacag cactagggtt tttaggaggg
      961 ttcactggcg aatttatttc gaagtggctt gaaaaagaac aaatccagtc tttgttcaca
     1021 accgtaaaag aagatacaag aatcaatatc aaactaaaat cagatactga aactgaaatt
     1081 aacggtttgg gcccatctat ttcagatgaa gaaatcgatg agttgaagca agcattagga
     1141 acagtaggac aaggagatat cgtcgtattg tcaggaagcg cgccggctag cctgcgaaaa
     1201 ggattttatc aagaactgat tgatatcatt cgagaaaaag gtgcagattt cgtgattgat
     1261 acaacaggtg atgacctaaa aaatgcactt gttaagaaac cattgttgat caaaccaaac
     1321 aatcacgaat tagctgagct ttacaataca accttccaat ctgtagaaga aatcattccg
     1381 tatggtaaaa aattgctagc tgaaggggca cagaatgtgc tgatctcaat ggctggagac
     1441 ggtgctttgc tcttcacgga acaaggaact tataaaacga atgtattagt tcgcccattg
     1501 aaaaattcag taggtgcagg ggattcaatg attgccggct ttattggggc ttacagccgt
     1561 acgaaagatc caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc aacagctttt
     1621 tctgatgact tggcagctcg cgaattgatt gatgagttgc tgccagaagt tgaaatcaca
     1681 aaaattgatt agtttcaagg aggataatca tgaatatcaa ggatttaatg gtcaaagaag
     1741 cgatgatcat ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa atggtccaaa
     1801 aaatgtttga tgccggccgg atttccgata tcgaaacata taaagaagga attctagccc
     1861 gcgaagcaca aacttctaca ggtcttggtg atggaatcgc catgcctcac gcgaaaaaca
     1921 gtgcagttaa agaagcaaca gttttattcg caaaaagcca aaaaggagta gattatgaag
     1981 ccttagacgg acagccaact tatctgtttt tcatgatcgc agctccagaa ggcgcaaacg
     2041 acacacattt gcaagcatta gcagcactat ctcgattgct gattgatgca gatttcgtag
     2101 gaaaattaaa agaagcaaat acaccagaag aagtccaaga cttgttccaa acagctgaag
     2161 agcaaaaaga agcagaagaa aaagcagaac aagaaacaca agctgcagaa gcttctgctc
     2221 atccagaaag aaaattcgtt gttgccgtta cagcttgtcc aacagggatc gctcacactt
     2281 acatggcaga agatgctctg aagaaaaaag cgaaagaaat gggtgtggag atcaaagtcg
     2341 aaacgaacgg ttcagaagga atcaaaaacc gattgactgc tgaagacatc gcccgtgcag
     2401 aaggtgtaat cgttgcagca gacaaaaaag tagagatgaa tcgctttgac ggaaaagaac
     2461 tgatcaatcg tcctgtcagt gatggtatcc gtaaaccaga agagctgatc aatctagccc
     2521 ttagtggtac tgcaccagta ttccatggtg atggaaaaga aagcagttca gaagaaggaa
     2581 atgcggatgg tactatcgga caaagaattt acaaagattt gatgaacggc gtatcacaca
     2641 tgttaccttt cgttattggt ggaggaatcg ctattgcgct ttctttcatg atcgaccaat
     2701 ttattggcgt gccacatgat caattatcaa atctagggaa ttacaatcaa gctgctagct
     2761 ggttcaacca aatcggacaa gctgcatttg gtttcatgct gccggtatta gcaggattta
     2821 ttgcttcaag tatcggtgac cgccctggtt tgatcgttgg atttgctgct ggtgcattag
     2881 ctaatacagg cggtgcagga ttcttaggtg cactgattgg tggattctta gctggttatg
     2941 tcatcgtatt tttacgtaaa ttattcaaga acttaccaaa atcattggat ggaattaaaa
     3001 cgatcctctt ttatccagtg tttggcttac tgattactgg tttcttgatg ttgctggtca
     3061 atgtccctat gaaagcaatc aatgatgcat tgaacggttt cttgacaagt atgagtggtt
     3121 cgaatgctgc tttacttggt gcattattag ctggaatgat ggcggcagat ttaggcggtc
     3181 caatcaacaa agccgcttat gtgtttggta cagcaacttt agctacgact gttgcaactg
     3241 gcggaagtgt cgtgatggct tctgttatgg ctggaggaat ggtacctcca ttggcaattt
     3301 tcgttgctac acgtttgttt aagaataaat tcagcaaaac agatcaagat gctggtttga
     3361 cgaacatcgt gatgggacta tcgtttgtca cggaaggatc gattccattt gcagcagctg
     3421 acccgattcg tgccattcca agttttatta ttggttctgc tttaactggc ggattagtcg
     3481 gagctttcgg gatcaaatta ctggctcctc atggcgggat tttcgttgta ttcttactga
     3541 gtcacccggt catgtatttg gtgtttattg ctatcggagc agtcgtatct ggtgtgattt
     3601 atggtttatt gagaaaagct cctgcagcag cagaagcata gaaataaaaa agaagaggct
     3661 ggtttttccc agtctcttct ttttttatag aatcatatag aatcaagtag tgtagatgaa
     3721 tagttgttgt aaaatagttt ttccatgttc gttaaaatga ttgcttcaaa tttgtgatag
     3781 aagatttagc ctcgatttcc tgcttgattc ttggcaagtt tgcttcatca gtcaagataa
     3841 ccaaaatatc tcctacattc atgatcgtat caccgtgagt caagatttcc tgatttcctc
     3901 tgcgtataga gattagcagc atatttttag gccaattgaa gtctcgtacc attaatccgt
     3961 ctaaagaact ctctgccgtt actggaaaat caaaagttgt ccttgcacct tttagtgagg
     4021 cggtctgagt atttccaagt agacgttcca ataatgattc gtaaattgga ttaccgccga
     4081 gaaggtcatt gacgatataa gcaacaagag cagccaatcc taaaggcatc agatgattaa
     4141 gagagccaac catttctgtc actaaaataa ttgctgtcag cggtgctttt ccaatcgctg
     4201 tgaaataacc agccatggca ataacgacaa aatccttgat gtagattgga tccatcccga
     4261 aatcaaccat aatgtttcca taaaagctac cgattaaagc ccccaatgtc aatatcggaa
     4321 ggaaaatccc ccctggaaga ccaccaccgt aagaaatcat ggaaaaaacg aatcgaagaa
     4381 caaacaaacc gataatcata atgattggca aggaagcatg tccaaaagct aaaacgattt
     4441 gattgcctcc gcctagagta ctaggccata aataaccaat tggcaaaatc agcaagaaag
     4501 gaacgaaccc gtgaaaataa gctggtaaag gcaatcggtt gtaaaatttt ggcaaagcca
     4561 gcaagacctt ctgataaata aaacctaaga tgcctagaag tactcctaag atcactaagg
     4621 aaccataata tttcaacggc aaagaaggaa ggtcacctag atataaaaca ggtctcaagc
     4681 cgaaaaagta aagagagacg aagttagcag tgattgctga agcaaatgat gtcagccaaa
     4741 caagtggaga aaaggagtga tggatttctt ctaaaacaaa gagtaatcct gcgataggag
     4801 cattgaatgc tgctgcaagt ccgctacttg caccgctaga aattaaaatc ttttcttctg
     4861 aagatggact ccgaaacaat tgtccagctc cttgtccaat ggatgcgcca agctgtatag
     4921 aaggtccttc tctacctaga aaaaggcccg caccaactga aagtacaccg ccaatgaact
     4981 ttttccataa aacagagaac cagttcattg ataattcccc gcgaagctgt ccctctactt
     5041 gtggaatccc gcttcccttg atatccggtt catttttgac cagataaccc acgaaaaatg
     5101 ctgcaaggat gcagcaaata gctaggccaa ttaagtaaat cggctgttcc cgactcattt
     5161 catagatact tcgaactaaa tcgcttagct tttcgataga taatcgaaac aggctaacga
     5221 cgattcctgt cagcagtcca acaaatattc cttttagaat aaaatatatc tgagtcttat
     5281 ctaatttctt gatttttcct tttttttcca ttcaaacgcc tcgctttctt ttaatttagc
     5341 gcgaaatcaa cgaattgaca aggaaaaatc cataaaaaga aacaaatgat tttgaactag
     5401 ctagtttaat gaaagccaag aaaatgctat aattaacaag attgataaaa gaaaggatgt
     5461 tgttaatgtc atccaaagct gaattattaa acgggatgaa cccccgtcag aaagaagctg
     5521 ttcttcacac tgacggacca ttactgctaa tggcaggtgc aggaagtgga aagacgagag
     5581 tgctgacaca ccgcattgcc tatttgattg aggaaaaaga agtcaatcct tggaacatcc
     5641 tagcgattac ttttaccaat aaggctgcaa aagaaatgaa agaacgtgtc aatgcgattc
     5701 tcgcatctgg aggcgaagat gtctgggtat cgacttttca ctcgatgtgt gtgcgtattt
     5761 taagaagaga tgttgatttt atcgggtata accggaactt tacaatcatc gattcttctg
     5821 aacagctgac cttgatgaaa cggatactaa aagaactgaa cattgatccg aaaaaatatg
     5881 atcctcgaag tatattagga acaatctctc aagcaaaaaa cagcctgcag acccctcagg
     5941 attttgcaaa aatgcaaggc agttattacg aagagattgc ggcaaaatgt tatgctgctt
     6001 accaaaaaga gcttcaatat aatcaatgta tggactttga cgacctgatc atgaatacga
     6061 ttcgattatt tgaagaacat cctgattcac tcacatatta tcaaaacaaa ttccattata
     6121 ttcatgtaga tgagtaccaa gatacaaatc acgcacagta tacgctagtc aatttattgg
     6181 caggtagatt taggaacctg tgcgtcgtgg gagatgcaga tcaaagtatt tacggctggc
     6241 gcggtgcaga tatgcagaac atcttggatt ttgaaaaaga ttatccagat gctgcagtaa
     6301 ttttgctaga acaaaactat cgctccacga aaaacatcct tagcgcagca aaccaagtca
     6361 ttgaaaacaa tagcaatcga aaaccaaaaa acttgtggac agaaaacaaa gaaggaaaca
     6421 agatcactta ctaccgtgca gataatgaac gagacgaaac ccgttttatc gtagatcgga
     6481 tgcaagaaga gattcgaagc aatcatcgga attacggcga ctttgccatc ttgtaccgga
     6541 cgaacgcaca atctcgtgta atggaagaaa ctctgttgaa agcaaatatt ccttataaaa
     6601 tggttggagg gcacaagttc tacgatcgta aagaaatcaa agatatttta gcgtatttga
     6661 atgtgctggc aaatcctcaa gattcaatca gttttgagcg tatcgttaat tcgcccaaac
     6721 gaggcatcgg accaggatct atcgaaaaac tacgcagttt tgcttctctt catgaatggc
     6781 cactgcttga agcagcgcaa aatgtggatc tggcaaatat cggtggaaaa gcaggacagc
     6841 aattaggtgc atttggagag atgatccaag aagtcactca gatgatccca tacttgacag
     6901 tgacagaatt gacgaaagag gttttggatc gtagcggata tctagaagac ttaaaaatcc
     6961 aaaatacatt agaagcacaa gcacgcatcg aaaacttgga ggaattttta acggtcacac
     7021 aagaattcga caaacaattc gaacagcaaa atgaagaaga cgctgatgca ccagaagaaa
     7081 aactaacggt cttcttaaat gatctggcac ttgtttccga tattgacaat ttagaagaag
     7141 atgcttccca agtgacattg atgactttgc atgcagccaa aggattagaa ttcccagttg
     7201 tattcctgat tggtctagaa gagggggttt ttcctttatc tcgagcattg atggaagaaa
     7261 gcgaactgga agaagaacgc cgtctcgcat atgtggggat cacgcgagca gaagaagcgt
     7321 tgtatttaac aaatgctttc tccagaacat tatatggacg gacgcaatat aaccgacctt
     7381 ctcgttttgt agaagaaatc gatcaggagt tactggaaat cgaaggcatg cgtcctacgc
     7441 ctaaaaaaac acctgtattc gctaaaaaaa cagcatatag ctataaacag ccagagacag
     7501 cggttgttcc ctctaaaagt gctactggag gggaaaataa cagctggaaa cctggagata
     7561 aagtcaaaca caaaaaatgg ggtctaggaa cagtcgtccg agtaagcggg acgtcgaagg
     7621 atctagaact agatgtggct ttcccaagtc aaggagtaaa acgtcttttg gctgcctttg
     7681 cgccaattga aaaagcttag tagtggaggg taaatgatgg aacatgaaat gacgattgac
     7741 gaagctgcta aacgagcaga agaacttcgt acaagactga accaatggtc tcgcgaatac
     7801 tacgtagaag ataaaccaac agtggaagac tatgtttatg ataaagaata cgcagaatta
     7861 gtagcaattg aggaacaata tcctgatttg atcacgtctg attctcccac tcaacgagta
     7921 ggcggaaaag tattagaagg ttttgaaaaa gtcactcatg atattccgct ttacagtttg
     7981 aacgacgttt tcagcaaaga agagctgata gcttttgatc aacgtgtaca aaaagcagtg
     8041 ggacgtgtag ttgactattg ctgtgaacta aaaatagatg gactttccgt ttctttgcgt
     8101 tatgaagatg gaaatttcgt gagaggtgcg actcgcgggg atggaactgt aggagaaaac
     8161 atcactgaaa acctaaaaac tgtccggtcg gtcccaatca aattaaaaga accaatgaac
     8221 atcgaagtaa gaggcgaatg tttcatgcca aaacggtcat ttgtccaatt gaaccaagac
     8281 agagaagcag agggaaaaga tatctttgcg aatcctcgga acgccgcagc tggttctttg
     8341 cgtcagttag attctaaaat cacagcgaaa agaaatctgg acactttttt atacacagta
     8401 gctgatttcg gtcctatgca agcaaaaacc cagtatgatg cgctagaaga gctagaaaaa
     8461 attggattcc acacgaatcg ggaaaaacgt ctatgtcatt cgattgatga agtgtggtca
     8521 tacattgaag aatatcatga caaacgtgtg gatttgccct atgaaatcga tggaatcgtc
     8581 atcaaagtga atgaattctc gctacaagat caacttggtt ttactgtcaa agcaccgaga
     8641 tgggcagcag cttacaagtt tccgccagaa gaagttgaaa cgctaatcga aaacatcgaa
     8701 tggacagtcg gtcgaacagg ggtagtaacg ccaacagcta tcatgacacc agtccgagta
     8761 gcaggaacga cagtaagccg tgccagcctg cataacggtg attatattaa gttgaaggat
     8821 atccgcttga aagatacagt tctgatttat aaagcgggag acatcatccc agaagtttcg
     8881 caagtcgtgt tagataaacg acctaaagac agcgaagaat accaattgcc tacacattgt
     8941 ccagtttgtg gaagtgaact tgtccatttg gatgaagaag tagccttacg gtgcatcaat
     9001 cctaaatgcc ctgctcaaat gaaagaaggt ttgaaccact ttgtttcaag aaatgcgatg
     9061 aatatcgatg ggttggggcc gcgcgtacta gaacagatgt atgataaaaa actagtagca
     9121 gatgttgctg atctttacaa attgacagaa gaagagttat tgacgttgga caaaataaaa
     9181 gaaaaatcag caaataatat tttgacagca atcgacaaca gcaaagataa ttcggtcgag
     9241 cggttgattt tcgggttagg aatccgacat gtcggagcga aagcagcaaa aattttagca
     9301 gaacatttcg gtgatctaga aacattgagt aaaagtgact atgagtcgat tattgcgctt
     9361 gatacgatcg gtgatatcat tgccgacagt gtagtcactt atttcagtaa cgaagaagtc
     9421 catgaattga tgaatgaatt aaaacaagca ggcgtcaatt tcgaatacaa agggcttcga
     9481 aatgcccaat tgcaagaagt cgagtctcct tttaaagaaa aaacagtagt ccttactggt
     9541 aaactaacac gatttactcg tgaagaagca aaagagacga tcgaaaatct tggcgggaaa
     9601 gtgactggta gcgtatcgaa aaaaacggat atcgtggtag caggagaaga tgctggcagc
     9661 aagctgacca aagcacaaga attaggaatc gaagtttgga cagaagatca aatggcagat
     9721 gcattggcta aaagtcgctc agtagaagaa tgaaaaatga gttttttcag acaaatttca
     9781 ataaacaatt tcagacacgg cactcttgtg ttaaaataga gagcaaagtt tttaactcgt
     9841 ttagaaagaa ggggtagtat ggcgattagt gaagaacaag tgaaacacgt tgccaaatta
     9901 gcaaaacttg cgttcgcaga tgaagaactt gcaagtttta ccgaccaatt aggtaaaatc
     9961 atcgatatgg ttgaaatgct ggaagaagta gatacagaag gtgttccatt tacgtcgaat
    10021 gtggttgaaa cagtaaacgt gatgcgacaa gatcgggcgc aagctggatg gaaccgcgat
    10081 gaattattga aaaatgtgcc ggaaacagaa gacggcttta tcaaagtacc cgcaatcatc
    10141 gataatgggg aggctggcgc ataatgacag aattacacaa tcgttctcta gaagaactgc
    10201 atcatatgct tgtatcgaaa gaaatcactg tacaagattt gacaaaagca acatttgaac
    10261 gaatcaaaga aacagagcct gaggtcgaag cattcattac attgaacgaa gaaaaagcat
    10321 tggaacaggc aaaagcttta gatgaaaaag ggattgatga aagcaacgtc cttgctggta
    10381 ttccaattgg tatcaaggat aatatcgtaa caaaagatat cttgacaacc gctgcatcta
    10441 agatccttta caattttgaa ccaatttatg atgcaacggt catggataaa atctatcaag
    10501 cagatatgat ccctgttggt aagctaaaca tggatgaatt tgctatgggt ggaagtacag
    10561 aaacttctta tttcaagaaa acaaaaaatg cttgggaccc aacaaaagta cctggtggct
    10621 cttctggtgg ttctgcagca gcagttgcag ctggacaaat tccagtttct ttaggtagtg
    10681 atactggggg aagtatccgc caaccagcag cttttaacgg catcgtaggg atgaaaccta
    10741 cttatggccg tgtctcacgt tttggtttga ttgcctttgc ttcatctctt gaccaaatcg
    10801 gaccaatgac ccgtacggtg aaagataatg cattagcatt gacagctatc agcggttatg
    10861 acgaaaaaga cggtacttct tcaggcgttt cagttcctga ttttgcagag ggactgacag
    10921 gcgacatcaa aggaatgaag attgctttac ctaaagaata cttaggcgaa ggtgtggccc
    10981 ctggcgtaaa agaagctgta ttgaaagcag cagaaacatt ccgttcatta ggtgctgttg
    11041 tggaagaagt cagcttgcct cattcaaaat acggtgtggc tgtttattat atcattgcct
    11101 catcagaagc aagttccaac ttgcaacgat tcgatggtat ccgctacggt tatcgctcag
    11161 aaaatgtgaa atcattagat gatgtatatg tcaactcacg ttcagaagga ttcggtgaag
    11221 aagtaaaacg ccgtatcatg ttaggtacat tctccttaag tgctggttat tatgacgctt
    11281 acttcaaaaa agcgggacaa gtccgtacat tgatcaaacg agattttgaa aaagtctttg
    11341 cagattatga cttgatcatc gggccatctt caccaacagt tgcattcgga atcggtgaga
    11401 acattaacga tccaatcaca atgtacatga gtgacatctt aacgatccct gtcaacttgg
    11461 ctggacttcc aggtatgtct gtaccggcag gtttatcaga aggattacca gttggattgc
    11521 agatcatcgg taattatttt gatgaaaaaa cgatgtatca agcggcatat gcgtttgaac
    11581 aagcaacaga attccataaa caacaaccag ctattttagg agggaaagac gaatgaattt
    11641 tgaaacagtc attggactag aagtccacgt cgagttaaaa accaactcaa aaattttctc
    11701 tccagcgcca gctcattttg gtgcagaacc aaacagcaat acaaacgtga tcgactgggg
    11761 ttatccaggc gtgctgccag taatgaataa agctgcgctg gaattcggga tgaaagcagc
    11821 tttggcatta aactgtgaaa tctctaaaga tactcatttt gaccggaaaa attactttta
    11881 cccagataat ccaaaagcat atcaaatttc ccaatttgat caaccaatcg gtcatgacgg
    11941 atggatcgaa atcgaagtag aaggtaaaaa gaaaaaaatc cggatcgaac gtgtccattt
    12001 agaagaagat gctggaaaaa acatgcatgg tattggcggt tattcttacg ttgatttgaa
    12061 ccgacaaggc acgcctttga tcgaaatcgt ttctgaagct gatatgcgtt caccagaaga
    12121 agcatacgct tatctagaag cattgcgatc aattatccaa tttacagaag tcagcgatgt
    12181 gaaaatggaa gaaggctcta tgcgctgtga tgccaatatt tctcttcgcc cttatggaca
    12241 agaagagttc ggtacgaaag cagaattgaa aaacttgaac tctttatctt tcgtgaaaaa
    12301 aggtttagct tttgaagaaa aacgtcaagc aaaagttctt ctttcaggag gagaaatcca
    12361 acaagaaaca cgccgttttg acgaaacaac gaataaaaca gttttgatgc gtgtcaaaga
    12421 agggtctagt gattatcgtt acttcccaga gcctgatatc ccgaaattcg tgatcgatga
    12481 cgaatggatt caaaaagtaa aagctagttt gcctgagatg ccagcttctc gtcgcgaacg
    12541 ctatatccgt gagtttggac ttccagagta tgacgcaatg gtattgacac ttacaaaaga
    12601 aatgtctgat ttttttgaag ctgttttgga aaatggtgca gatgccaagc aagcatctaa
    12661 ctggttgatg ggtgaagttt ctgcttactt gaacagtgag aaactggaat tagctgagac
    12721 aaaattgaca cctgagaact tagcaggaat gattcgtttg atcgccgatg gaacgatcag
    12781 ctcaaaaatc gccaaaaaag tcttccgtga attgatcaca aatggcggag aagcccaaga
    12841 agtagttgaa aaaaatggtt gggtgcaatt atctgatccg aacaaactac tgccaatcat
    12901 taatgaaatt ttggataaca accaacaatc tgtcgatgat ttcaaaaatg gaaaagatcg
    12961 tgcggttggc ttcctagtcg gtcaaatcat gaaagcaact aaaggacaag caaatcctgg
    13021 tgtcgtgaat aagctactta aagatgaatt agcgaaacga tagaggctga aaatgaaaaa
    13081 agcacgcgta atttataacc ccacctcggg aaaagaatta ttgaaaaaaa atttggcgga
    13141 tatcctacag gcattagaag aagcaggatt tgaagccagc gcctatgcaa ctacaccaga
    13201 accagattca gccaagaatg aagcatgtag agtcgcagaa ctaggttttg atctagtagt
    13261 ggcagctggt ggcgatggca caatcaacga agtggttaat ggaatcgctc cgcttgaaca
    13321 ccgtcctaaa atggccatca tcccagctgg tacaacgaac gactatgctc gagcattaaa
    13381 aattcctaga gataatatta aagcagcagc agaagtgata aaaaaacacc aaaccgtaaa
    13441 aatggacatc ggcctttctg ataacagtta cttcatcaat atcgctgcag gtggctattt
    13501 gacagaattg acttatgaag ttccatcaga actaaagagt atttttggtt atcttgctta
    13561 tcttgcgaaa ggagcagaga tgctccctcg agtaaagcca atcaaaatgc gcttgaagta
    13621 tgatgaaggg gaatatatcg gaaatgcttc gatgtttttc ctagggctga cgaactctgt
    13681 tggaggattc gaacagatcg cgccagatgc caaattggat gatgggaagt tttctttaat
    13741 cattgtgaag acagcaaata tctttgagat cttgcatatt gcagcactca tgctgaatgg
    13801 cggtcgacat gtcgatgatc caagaataat ctataccaaa acaagcagtt tgttcgttga
    13861 aacagaagat gatatcaatc gaccagtaat gatcaactta gatggtgaat acggcgggga
    13921 agcaccgatg catttccaaa atcttcatca acacattgag ttctttgcca ataccgatca
    13981 gattcctgat gaagcaatca caggtacaga tgaagaagaa ttagaggttg tcagcaaaga
    14041 attcgtaaaa gaagtcgaac gtttaacgga tgaagatatc gacggtgacg gaaaaatagc
    14101 agacgaaaca cctaatcaaa agaattaatc aaaagccggt gctgaacgta gtaaggtcag
    14161 tgccggtttt agttgtgtaa ttagctgatt tatagtagag ttcagtagga ttgaaaaata
    14221 ggcttaaagg agcaaaatat gacagaacag caaatagtaa agaaaaacga acgatataca
    14281 gtcgaaatcg tcgacttgag ttatgaagga atgggggtag caaaaatcga tggttatcca
    14341 ttatttatcg aaaatgcact ccctggagaa gaagtagaaa tactagtagt aaaagcaggc
    14401 aataagtttg gatatggaaa agtcgaaaca tttattacac aatcacctga tcgtcagcct
    14461 gtagaaaaca atgcattact tcgcataggt atcgcacctt tagcccattt gaagtatgaa
    14521 caacagcttg tcttcaaaca aaaacaagta gagcatgtaa tggcgaagat tgctaagatg
    14581 ccagaagttc ctgtacttcc aacggtcgga atggcagaac cattcggcta ccgtaataaa
    14641 gcacagatcc ctgttcgccg tatcgatgga cagttagcca ccggttttta caaaaagaat
    14701 agccatgatc tagtccctat cgaagatttt tatatccaag atcctgtcat cgatcaagca
    14761 atcaaagtag tcagagatat cttgcaacgg ttccaagtac gaggatataa tgaagaaaaa
    14821 aatgaaggac agcttcgtca catcattatc cgtcgtggtc attattccca tgaaatgatg
    14881 gtcgttttag ttactcgtaa agaaaaattc tttaaatcca aggaaattgc tgaagctatt
    14941 catgaagaat tgccagaagt ggtttcggtc atccaaaata tcaatgaaga aaaaacaaat
    15001 gtgattttag gagacaaaga aaaagtttta tatggcagag actatatcga agatcagcta
    15061 ttaggaaaaa cctatcgaat ctcagcaaag tcattttatc aagtaaatac tcaacaagca
    15121 gagtatctgt accaaacagc catcgagttt gccgagttag ataaaaaaga cattgtgatc
    15181 gacgcttatt caggaatagg tacgattggc ttgtctttag cagacagagt ggataaggtg
    15241 tatgggatgg aagttattcc agaagcaatt gaagatgcac agttcaatgc tttaaataat
    15301 aaaatcgaaa atgttcatta tgaagtcgga aaagcagaaa aagttatgaa aaaatggcaa
    15361 gaaaaaggga tcaaaccatc tgtgatcgta gttgaccctc cacgaaaagg attagatcat
    15421 cattttattg aatcagcaat cgaaatgtca ccagaacgaa tcgtctatat ctcatgtaat
    15481 ccggcaactt ttgcaagaga tgctaagtta tttgcagaag caggttatgc cgtgaagaaa
    15541 gttcagcctg tagatctttt cccgcaaacc catcatatcg aattagtcgc ttcattcgtt
    15601 aaaggagaat aaaaatggac caaaaagaaa aaatgaaaga attgatcaaa aagaaacaag
    15661 gtggaggccg atccaaacaa atggaaacac ctaagaatga tcgtaaaaac atgagaaaag
    15721 gccctaaaat ctttaataaa tagacgtgct aaagaaagac taagaagtgt taacttttgt
    15781 tattgtttct tagtcttttt a
//
LOCUS       DAYYKN010000060        15321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000060 DAYYKN010000000
VERSION     DAYYKN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.060"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..570)
                     /locus_tag="ACHBB7_001611"
     CDS             complement(112..570)
                     /locus_tag="ACHBB7_001611"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM9209568.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(741..1886)
                     /locus_tag="ACHBB7_001612"
     CDS             complement(741..1886)
                     /locus_tag="ACHBB7_001612"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM9209569.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1899..2816)
                     /locus_tag="ACHBB7_001613"
     CDS             complement(1899..2816)
                     /locus_tag="ACHBB7_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM9209570.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3244..3807
                     /gene="efp"
                     /locus_tag="ACHBB7_001614"
     CDS             3244..3807
                     /gene="efp"
                     /locus_tag="ACHBB7_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM9209571.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            3908..4078
                     /locus_tag="ACHBB7_001615"
     CDS             3908..4078
                     /locus_tag="ACHBB7_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209572.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4293..5552
                     /gene="lysA"
                     /locus_tag="ACHBB7_001616"
     CDS             4293..5552
                     /gene="lysA"
                     /locus_tag="ACHBB7_001616"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM9209573.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            5539..6339
                     /gene="dapF"
                     /locus_tag="ACHBB7_001617"
     CDS             5539..6339
                     /gene="dapF"
                     /locus_tag="ACHBB7_001617"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM9209574.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6342..7223
                     /gene="dapA"
                     /locus_tag="ACHBB7_001618"
     CDS             6342..7223
                     /gene="dapA"
                     /locus_tag="ACHBB7_001618"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM9209575.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7220..7936
                     /gene="dapB"
                     /locus_tag="ACHBB7_001619"
     CDS             7220..7936
                     /gene="dapB"
                     /locus_tag="ACHBB7_001619"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM9209576.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            7942..8637
                     /gene="dapD"
                     /locus_tag="ACHBB7_001620"
     CDS             7942..8637
                     /gene="dapD"
                     /locus_tag="ACHBB7_001620"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM9209577.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            8653..9654
                     /locus_tag="ACHBB7_001621"
     CDS             8653..9654
                     /locus_tag="ACHBB7_001621"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM9209578.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            9651..11012
                     /locus_tag="ACHBB7_001622"
     CDS             9651..11012
                     /locus_tag="ACHBB7_001622"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM9209579.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            10972..12123
                     /locus_tag="ACHBB7_001623"
     CDS             10972..12123
                     /locus_tag="ACHBB7_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9209580.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12098..13267
                     /locus_tag="ACHBB7_001624"
     CDS             12098..13267
                     /locus_tag="ACHBB7_001624"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9209581.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13442..14821
                     /locus_tag="ACHBB7_001625"
     CDS             13442..14821
                     /locus_tag="ACHBB7_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9209582.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15102..15321
                     /locus_tag="ACHBB7_001626"
                     /pseudo
     CDS             15102..15321
                     /locus_tag="ACHBB7_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5019 a         2719 c         3277 g         4306 t
ORIGIN      
        1 aaaactagct atttgttttc ccgtttagaa ctcttttcag acaataaata tttaataaaa
       61 tacgaagata agatagctat attgcttctc atctttacgt tcttttagaa actatgcttc
      121 tacttcagac cattcatcgc gttttgctaa aaattcacga gccaaagctt gtgcaccttc
      181 tagtgtatgg ctggcagccc agccacattg tgtttcattg cttgcaggta cttcagtagc
      241 ttcaagaaca tctttcattg ttgcttcaag aacatctaaa atgtcttcgt agctttcatg
      301 attcaataca gtcaagtaaa agcctgtttg gcatcccatt ggagaccagt ctacgatgta
      361 atctgcgtga ttgcggatta gttcggcagt caagtgctca agtgaatgta gacttggcat
      421 gtccatgtgt tctttatttg gttgtttaaa gcggacgtcg tatttaatga tgacatcgcc
      481 atgcgcaccc gtttttcgat cagctacacg tacataaggt gctttgactt ttgtatggtc
      541 aagattaaaa ctttcgacat tcattctcat ttgtaagact cctatctgtt tgttcattcg
      601 ttttgatgct tttcactaaa gaattattta gtgaaattta caaaaacgtc tttctacttg
      661 attctaacac ttatcgtttc caaggtgtag caatctgaaa tattttttag atttttctgc
      721 taagtcgttt attgggactt ctagttcttg attctgtcaa atgcctgagc taaatcgttt
      781 atgagatcct gaccatactc aattcctaca gaaagacgga tcaagccgtc ctttatgcca
      841 gcctcttctc gtttttcttt tggaatagac gcatgagtca tgacagaagg aatttccact
      901 aaactttcta cgccacctaa actttctgct aaagtaaata gttggagtga ttcaacaaat
      961 ggaatagcag attcttcatt ttttaaagta aaagaaatca ttccgctaaa gccgctcatc
     1021 tgctgcttag caactccatg gagttcatga tcgggtaatc ctggatagta gaccttttct
     1081 actgctggat gagaaaatag aaaatcagct accacaaaag catttttttg atgttcctcc
     1141 atacgaacac ttagtgtctt gattccgcgc tgaagtaacc agctatcttg gggaccaagt
     1201 acggctccga tagcattttg gtaaaatccg atctgatcgg ctagatcttt atggttagaa
     1261 gtcactaaac cagctactac atcgctgtgt ccgcctagat atttggtacc gctatggaca
     1321 acgatatcag aaccgagatc taaaggtctt tgaaaataag gagtagcaaa tgtattgtca
     1381 gcaataacta tcaatccatg ttgatgagcc agagtggcac ttttttctaa atcagtaatc
     1441 tttagaagcg gattgctagg tgtttcaaga taaagtgctt ttgtattggg tttgatggat
     1501 tgttctattt tatcaagatt actcgtatct atgatcgtgt actcgagacc atttttagta
     1561 agaactttgt caaatagacg gaatgttcct ccatagacat catctcctaa taagatatga
     1621 tcgccagctt gaaaaagaga aaaaacggca tgaatcccag aaagaccaga agaaaaagcg
     1681 aatcctcgaa caccgccttc caaatcagca atcagttctt ccaatgcaaa acgagtagga
     1741 ttaccagatc ttgaatattc atattgttta ggctggccga cgccatcttg ccgataagtg
     1801 gatgtttgat aaataggtac actgacagct cctgttgtgg gatcttcact gatgcctccg
     1861 tggatcaatt ttgtttgaat gtgcatgttt ctttcctcct ataagtaaat gttttttgat
     1921 aaatagcggt ctgctgcgtc tggaaaaatc gtaacgacac gatgaccagg tggtaaacga
     1981 cgaatttctc ttaatgctgc tgcaaaagcc gcaccactcg aacttccaac taatagtcct
     2041 tgttctcgtg ctaactgcct agtatagtta aacccttcta catcagagat tgtctcgatc
     2101 tgattgattg aaaggggaga aagaaacggc ggaatgaatt ctacaccgat accctcaatt
     2161 tcgtgagggg ctggatcacc accatttaac acagagccct ctggttctac accgattatt
     2221 cgaatgtcgg gatatttttc tttcaaaaag gtagaagttc cagcaaaggt tccaccactc
     2281 ccaattcctg ctacaaaact atggatattt tctttgattt catgaaagat ttctggccct
     2341 aacgtatgat agtaagcagc aggattatct ttattttcaa attgaagagg cagatagctg
     2401 tttgatatct cctctgcaag ttttttactt ttgctgatgg caccgatgat tccttgctca
     2461 ctaggagaat ggatgatctt tgctcccaat gctttcatca attgttgttt ttctaagcta
     2521 aacttctctg gaacaacaaa aaccgttctt aatttgtatg tgagtgcggc cagagcgatc
     2581 ccaattcctg tatttccggc agttggttcg atgatcgtcg ttttttcatt gatttttcct
     2641 tgggcaaatc cttctttgat caaataactg cccaatctat ctttgatact tcctccagga
     2701 tttaaatatt ctagttttgc ataaatagca gaacctttag gcatagaata gtgctgattg
     2761 tcaaagcgat aaagcggtgt ggaaccaata gcttctgtaa ttgtcgagat aatcatgtgg
     2821 gcctcctaaa aaaagataat aaaaaaagga gctgactatt aaatcagctc cttgaaagac
     2881 atagagaaaa attgagtaga taaaaagata agttacctgg tactcattct atgaagggca
     2941 attaatagac atgacaaata gtatgctgct tttatgcacc atacaacagc aacgattcaa
     3001 ttgtttttgg ttgctgactg tcatgtgtat ccctccaagt aaaattttta ttaacgatag
     3061 catgaaaaaa aataaaagtc aaaggcttga aagaaaaaaa tatttcgata gcatcgttta
     3121 cccgtgttta tttttctgac aaataggcat gagaccttaa aagatagaga atactctagt
     3181 tttctggccg gttgtctgat acaataaagt tgattatgat aaagaaaaga aggagcaaaa
     3241 ataatgatct cagcaagtga tttaagagcc ggtatgactt tcgtacaaga cggcaaactg
     3301 attaaagttc tggatgcaag ccaccataaa cctggtaaag gaaatacagt tatgcgtatg
     3361 aaactgaaag atgtccgttc aggtgcaaca tacgacacaa cgttcagacc agaagaaaaa
     3421 tttgaaaaag cacatatcga cacaaaacct gtacaatatt tgtacacgat ggatgataca
     3481 gctttcttca tggatttgga aacatatgag caatatgaaa ttcctgtaga aacagtagaa
     3541 gaagaaatga aatatatcct agaaaacatg gaagtaaaaa tccaattttt cggtagcgaa
     3601 gtcatcggcg ttcaattacc aacaactgtc gtattacgtg ttgtggaaac acaaccttcc
     3661 atcaaaggtg caacagtcac tggttcaggt aagccagcga caatggaaac tggcttagta
     3721 gtcaatgttc ctgacttcat cgaaacagat gaattgttgg aaatcaatac agcagaaggt
     3781 tcttatgtaa aacgtgcgtc aaaataattc gctaacggat tgataagaat ttgaaaacag
     3841 agcttgacct cttgaaaggc agatcatcgg atctgccttt caagaggttt tttgtggaat
     3901 cagcaaattg aaaaataaaa aagtatttga ttttatttat attttgaaag atgaatggca
     3961 aaagctagag aaagaaaaaa ctgcacttat ttatgaagaa attgaaatga ttcataaaat
     4021 tgaaaatatc cgaactattc cgaactatat tgaagcgaaa atattttttc atcactaaat
     4081 agtgttgaat ttatgttttt atgtataaat agcttctaat agttcgggta ttttgttttt
     4141 ataaccattt ttattcttat ttttaaaaaa atatttcgaa atatactaga caattcagaa
     4201 aattttgttt aatattattc caataaaatt caaatcttta tcaaatgaaa ttccaataaa
     4261 aacggaaaaa aagtgaaaag aggaatgaaa aaatgacaga attgataatt ggcggagtca
     4321 cagcaagcac attggcagaa aagtataaga cacctctgta tgtgtatgat gaagaaaaga
     4381 tgagacaaac gatgctgtca ttcaagcgag gctttgtatc gaaacgtttt gatacaaaag
     4441 tgctttacgc ctctaaagca ttccaaacga ttgagatgct gcatttagtt cgtgaatatg
     4501 gattcgggct agatgttgtc agtggtgggg aaatctatac cgctttgcaa gctgattttc
     4561 ctagagatac tatctacttc catggtaaca acaagacgcc agaggaactg atctatgcac
     4621 ttgaaaatga tttgttgcat attgttgccg ataatttgat ggaagttgaa ttattagcag
     4681 aattaagcca acgctattta aaaaaaatct gtatcatgct tcggttgaat gtcggagtag
     4741 aagcacatac tcatgaatat atcgtcactg cgcatattga ttcaaaattc ggcatgagct
     4801 atgaaagtaa agagtgccaa caagcccttc agttgataaa agaaagcagc tatctagagt
     4861 tagaaggatt tcatgctcat atcgggtcac aaatttttga tgttactgct tggttagctg
     4921 aaatcgacaa attgacgaat tacttaaccg attttgacca acctctttcc ttgaatcttg
     4981 gcggagggtt tgggattcgt tatacacaaa atgatcagcc gatcccagtc gaagaggcac
     5041 taaaaaaact gattgaatat acggaagaag ctttggaaaa acgaaaactg accattcgtc
     5101 aattactgat cgaaccgggt agaagtattg ttggagaagc tggtacaaca ctatacacga
     5161 ttggtttcat caaaaagaca ccacataaaa aatattactt tgtagatggc gggatgacag
     5221 ataatatccg tccagctctg tatcaggcag aatacgattg tgatttagca gcaaaactgt
     5281 cggaagaaaa aacagagaaa gtgacgattg caggaaaaat gtgtgaatca ggcgatgtgg
     5341 taattaaaga agcttatttg ccgaaagcag aatcgggtga tcttttagct gtttattcca
     5401 cgggagctta tggatactcg atgagcagca actacaatcg agcgacacga ccagcagttg
     5461 tttttgtcaa taatggccaa tcaagactga ttgttcgcag acaagcattt tctgatctac
     5521 taagggggga agtttcctat gatccttcat aaatataatg gctgcggaaa tgattttatc
     5581 ttattagatt acgcagaaga cacagattat agtcaattag ccgcttcttt atgcacaaaa
     5641 gaaaaatatg atatagacgg attgatcgcc gtaaaaatcg atccgttgga aatgatctat
     5701 tacaacaaag atggcagccg cgcgccgatg tgcggaaatg gcattcgctg ttttgctcga
     5761 tacgtaaatg atcagaacat gatgaatgaa caggaattca acgtagagac attagcaggc
     5821 acaagaaaaa ttacggtgtt ggatccctca cctttttatt gcagcgtaga tatgggagtg
     5881 ccagatgatt ctccggaaaa aatcaaagct aatcaaactt atccaataaa aaaccaggaa
     5941 atagagatca atggtgaaaa agtaacgatc actagtttat ttttaggaac gattcatacc
     6001 gttgtttttg ttgaaaatgc gcgtgaggaa gtctggagaa aaagaggtga actgatctgt
     6061 catcatcctt tgttcaaaga aaaaaccaat gtcaactttg tagaagtagt caatgaacaa
     6121 gaattgatcg tccgtaccta tgaaagagga gtaggttgga cacttgcttg cggtacaggt
     6181 tgctgtgcct cttatgtggt tgctaaggaa actgggaaaa tttcagcaga tcaggtagtt
     6241 gttcacttgg aacaaggaga cttgatgatc tctggtgagg atgcgataca aatggctggc
     6301 ccagcagttc atgaatggac aagaatattg gaggaatgaa gatgataaaa ggttcgatcg
     6361 tggcgctgat cacgccgatg aatgaagagg gatcagtaga ttatgctggt ttggaaaaat
     6421 tgatccaatt ccatttagat gaacaaacag atggattatt agttttaggt acaacaggag
     6481 aatcttctac gctcacacaa tctgaggagg agcagatcct tcaactgaca gtaaagaaag
     6541 tggctggccg tgtgccggtg attgcaggag ctggaacgaa taatacgaaa gaaacgattg
     6601 aaaaagcgaa gcattttgct tctttggggg cagacgcact tttggtcatc accccttact
     6661 acaacaaaac aagtgatgca ggactagctg ctcattttac agcaattgca gaagcctcac
     6721 cgattccgct tattttgtat aatgttcctt ctagaacagg catgtcgatt ccaatccatg
     6781 tattagtaaa tttagcagaa catcctaata tcattggttt gaaagaagcc tcaggagata
     6841 tggcttatgt gatggatgcc gctcggttga ttggagaaga attcttcctt tacagtggaa
     6901 atgatgattt gatcctacca gtaatgagtg taggaggaag cggtgtaata agtgtatggg
     6961 cgaatatcca acctaaaata gtccatgaat tagtaaaaga tacgcaggac ggaagatggc
     7021 agcaagcaaa agaaaagcaa ttaaatgcac ttgaactgat ccatgccttg ttcagtgaga
     7081 ccaatcctat tcctgtcaaa gcagccatgt cattattaga cttaccttcc ggacctctac
     7141 gcctgccttt agtttctctt tcaaaagaga aaaagaaaca attagctcag cttttgttaa
     7201 aagaaaggac ggttgaaaaa tgaaaatcgg actgattggc agtggacaga tgggaaatag
     7261 agtcgaagaa gttgcacaag aaagaggaga tacggtcctt cttttacgga cggcacctaa
     7321 agaagcaact acgcttgctt ttacggaact gccagaactg cttattgatt tttctcatcc
     7381 agataattta gagaaaatcc tttcgtatag cttatcatat caacttccgt tagtcattgg
     7441 cacgacaggt tatacacaat cacagttcca agaaataaaa gaacatgcca aacatgtacc
     7501 tgtgatgtat agtgctaatt tttcgttcgg catcatggtg atgaatcaac taatcaagca
     7561 agcagctgca atgttgcaag gctggcagat cgaactgatt gaaaaacatc atagtcgtaa
     7621 aaaagatgca ccatctggta ctgcaaatat gctgcttcaa acaatccaag aagtgcaaaa
     7681 gctgcagccc gtatacaact ggcaagggaa aaaaagagaa gaaaaccaaa ttggtgtaca
     7741 cagtattcgt gcaggttctt tacctggtga acatgatgta ttgtttgctg ctactgatga
     7801 aatatttacg atcaaacatg aatctttttc taatcgaatc tttgctgcag gcgcagtaga
     7861 agcagcggac tggttgaaag atcagtcacc aggttattac aaattagaag atttattaat
     7921 ggacaaaggg gtgtaaaaaa cgtggaagct caagaaatca tccgctacat tcaaacggca
     7981 tctaaaaaga cgcctgtgaa agtctatctg aatgtaaccg agccaatcga atttaaagaa
     8041 agtaaagttt ttggtgaagg aagcagcttc gttgtttttg gtgactatga gacgattgct
     8101 ccggttttag aagctgaatc agaaaaaatc attgaaatcg aaatcgaaac tgctgcaaga
     8161 tacagcgcag ttcctttgct agatattaaa aaaattaatg ctcgtatcga accaggtgct
     8221 attatcagag atcaagtgtc aatcggcaac aatgccgtga tcatgatggg agcgattatc
     8281 aatattggtg cagtaatcgg tgaaaacacg atgattgata tgggtgctgt cttaggtgga
     8341 cgagcgacag tgggcaaaaa ctgccatatt ggtgcaggag ctgtcttagc aggtgtgatc
     8401 gaaccagcat cggccaaacc ggttatcgta gaagacggcg tactggtagg tgcgaatgca
     8461 gtgattgttg aaggtgtcca tatcggaaaa gatgcagtcg tggcagcagg agcagtggtt
     8521 ttagaagatg tggcagcaga aactgttgtc ggtggaatac ctgcacgtgt attaaaaata
     8581 agtgatgatc agacaaaaga aaatacagca ttgattgctg cgttacgcga attatagagg
     8641 agatggagaa aaatgcgcga aatagatacc tgtattgtgg gggcaaccgg attagtcggc
     8701 caaacgatgc tcaaagtgtt agaagaatat gattttccag taggacgatt gaaattatta
     8761 gcttcaaaac gttcagccgg taaagtttgt gtattcaaga aaaagaaaca cactgtacag
     8821 gaattgacag aaacttcatt tgaaggatat gatttagcgc tattttctgc aggtgcttct
     8881 gtatccaaag agtttgcgcc aatcgcacga agtcagggac tatttgtcat agataattcc
     8941 tcagcatggc gggaagattc ggatattcca ttaatcgttc cagaggtcaa tctggaagac
     9001 tatacattga atcggctgat tgcaaatcca aattgttcaa ccattcaagc ggtacttccg
     9061 cttaaagctt tacaagaagc ctttggtatc cagcgagtca attattcgac ctatcaagca
     9121 gtttctggtt ccgggcaaaa aggcattgaa gatcttcatc gtacgcaaaa tgacgaggaa
     9181 ccagttttgt atccttataa tattagccaa acggtgattc cggaaatcga tcagcctttg
     9241 gaaaatgggt atacaaaaga agaacagaaa atgataaatg agacccgtaa gattctgcat
     9301 cagcctgctt tgccagtatc agcaacctgc gtacgtgtgc caatcgaaaa tggacatgga
     9361 gtctctgtgg cagttcaact gcaaaaagag tttacgacgg aagaaattag agagtgtctc
     9421 agccagtttc cagggattca attagaagat gatctagcag cacataaata tccgacgagc
     9481 attcttgcta gaggaacaga tatggtttat gttgggagga tcagaaaaga cacttcttta
     9541 gagaacggat tgttgttcta tacgactgcc gataacatac gcaaaggagc cgctgcgaat
     9601 gccgtacaga ttgctgcagc tttaatgcag aaaatagcgg aggtggcaag atgagtaata
     9661 cgctgaaagt agcgaaattc ggcggaacat cgatgtctca tgaagagcaa ttcaaaaaag
     9721 tccgtacaat tgttctttca gatcccaatc gccaagtgat cgttgtttct gctttaggca
     9781 aacgtacgtc agatgatgac aaagtaacag acttacttta tttgatttat gcttaccttc
     9841 agcatcatgt tgattggaca cccctttgga aaaggatttg tgatcgtttc attcaagtaa
     9901 gagatgttct tgccctgcag ttacctattg aagatgaact agcacgcata cagaaacaac
     9961 tggaagacga aaagataact gaagattatt tagtaagtag aggtgaatat ctgactgctc
    10021 aactgatggc tgagtatctt ggttaccagt ttattgacgc aaaagacctg attttctttg
    10081 actatgatgg gaaaatcgat caggaaaaaa cagcacagga aatcacaaaa gcatttaaaa
    10141 gatatccgaa aatggttgtt ccagggtttt acggggcgaa ccctgcagga aaagaaaaat
    10201 tattgggccg tgggggaagc gacattaccg gcgcgatact ggcgaagatg ttgcaagcgg
    10261 aaagttatga gaattggacg gatgtttcag gaatcatgat ggctgatccg cgaatcatcg
    10321 atcatcctaa aatcatcgag gaaatctctt ttagagaact gagagagatg gcttatatgg
    10381 gggctaacgt tcttcatgaa gcagctgttt tcccagttca agaggctgat atccccatcc
    10441 aaataaaaaa taccaatgat cctttcgctc aagggacaaa aatatcgaat cacgaaatag
    10501 aaaaggaaaa tggactgaca ggaatagctg gacgcaagga ttttctttcc atcactttat
    10561 tcaaacgtca tatgtccgat gaaatcgggt ttatctggaa agcaatgagc atttttgcta
    10621 aacatgagat atcaatcgaa catattcctt cagggatcga caatatcgga gtgatcgtct
    10681 cagcagaagc gattgccgat aagttatttc tcatcacgaa agaattaaaa gaaacacttg
    10741 gcgtggaaga aatcgtcgtg atcgaagact tagctttgat ctctgtcgtt gggggtccgc
    10801 ataaagaatt gattggatta tctgggaaag tgttatcgat attgaacaaa ttagaaattc
    10861 gcacatcgat cttatcacaa ggagcacagg agttgaatct gatcatcggt gtaccgaata
    10921 atcaatatga aacagtagtg aagggtatct atgaagggat ggtgaatacg gatgctgaaa
    10981 gaatatcgaa tggcactcca tcaaattcct gaaatcgggt ttcatgaatt caagacaaaa
    11041 aaatatttat atgaacaagt aaaaaacagc gaggccgtta tccatgaaat cggcgaaaca
    11101 ggactactcc tttattttga taatcagcaa gaagaaacga ttgcttttcg aacagatatc
    11161 gatgcactgc cagtcaaaga agaaacgaat ctctcttttg cttctaagca tgaaggttat
    11221 atgcatgcat gtggtcatga cggacatatg gcgatgcttt tagggttgac tgactttata
    11281 actaaacaca ttgaggatct tatttataat gtagtactga tctatcagcc atcagaagaa
    11341 atttccggtg gcgcacaaag cgttattcag tcaggattgt tggataaata tgaagtacaa
    11401 gcaatatttg gctttcatct atggcctgga ctagctaagg gagaagtttt ctctcgacca
    11461 ggacctttga tggcacaaag cagtgaaaca gacattatca ttaaaggcca gtctgcacat
    11521 attgcttcta gtgaaaaagg aatcgatagt ctggaggcag ctgtacgatt tttgaagaat
    11581 gtttatgatt ttgaccggca attaccggca gagtggaatc atctaatgaa atttggccaa
    11641 atccaaggtg gaacgatccg aaatgtttta gctggagaag ttattgtatc cggaagtatc
    11701 cgttcttaca gttcagatat gcaaaattat ttgaagaggg gattagacca tttagcagaa
    11761 cagtttcact ctacagctac tatttctttt cgctataatg acggctatcc agctgtaatc
    11821 aaccatagag gcttatatgc agcactgtta gattcaaaca aactacatga attagatgct
    11881 ccagttcttc aagccgaaga ttttggcata tatacgcaac aatatccatg tgtatttttc
    11941 tttttaggtg tcggtgatac ccctgcttta catgatccga cgttcgattt tgatatggat
    12001 gttttagaaa atggtgtaga atggtacaaa acgattcttt ttacagaaaa tcttcttgta
    12061 aaaatcgata gcagttcaaa agaggagcga gtaaataatg atgaatttat ccaatcgatt
    12121 tagtcctagg ctttctagaa tagaagtatc aaaaatacga caattcgatc agcaaatatc
    12181 gtcgatacca gatgtgatca aactcaccct gggagaaccg gattttccaa ctcctgaaca
    12241 tgtcaagcaa gcaggaatag cagcgattga agaagacttt tctcactata ccggaatgag
    12301 aggattagag gaactgcggg aagcggcttg tatatttcag caacagcgtt atggattaac
    12361 ttatgatccg caaacggaag ttttgacgac agttggtgca acggaagcga ttgcctctgc
    12421 gttgttatct gttttggaag aaggagacaa ggttcttatt ccagcgcctg cttattcagg
    12481 atatcagcca ttagtggaac tagctggtgc tgaattgatc ccgatagata cttctgacac
    12541 agggtttgtc tgtcagccgg aacaatttga acgagcattt gaacaatatg gatcagctgt
    12601 caaagcagtc attttaaact atcctaacaa tcctactggg acgacattgt ctgcaaaaca
    12661 attgaaagca atcgctgagg ttttaaaaaa atatccggta tttgttataa gtgatgaagt
    12721 atatgctgaa ttgacttata gtggaacgca tatgtccata gctacttatc ttccagaaca
    12781 aacaatcgtg ataagcgggt tatccaagtc acatgcgatg actggttggc gagtgggctt
    12841 tatttttgct caaaaagccc ttattgacga actaattaaa gtccatcaat atctggtgac
    12901 tgccgctaca acgatgtcgc aaaaagctgc ggctgaagcg ctaataaatg gggtgaaaga
    12961 tagcgagaat atgaaagagc aatatcaaac acgaagagac tatctggtcc agcaattggc
    13021 gcctttagga tttgaagtga cccagccaaa cggtgcattt tacttgtttt gcaaactccc
    13081 agctgccatt caaactgact cttggcagtt ttgtgtagaa ttagcggaac aggcaaaagt
    13141 tgcgtgtatc ccaggaattg catttggccc agaaggagaa ggttatatcc ggatttctta
    13201 tgctagcagc atggaaaaat tacatgaggc ctgcaaacgg atcaaagact ttttaacaga
    13261 acagtgattc atagatttta tcaaaaaaac aatcgttaat tttgcggtat attctgcaag
    13321 gagacgattg ttttttaaat ttagttcaaa tgaacgcgtt tcttttgatt cataactaat
    13381 gtaatgttaa gtttaaattg tttagaatga ttttaaaaga atattcaagg aggtttcatc
    13441 aatggaaaaa atcaaagttg tcattgtagg tgcatctcat ggagggcatc aatcgatctt
    13501 ggaactattg acacgatacg gtgaaaatgt agatatcacg ctatttgaag caggagacta
    13561 tatttccttc atgtcatgtg gtatggagct gtatttagaa gacaaggtaa cagatgtaaa
    13621 tgatgtacgt aatttcagac cagaaaactt tccgcaatcc aatgttcata ttttaaataa
    13681 tcacgaagta aaaacgatcg atgccgataa aaaaacagta acggttacta gcgcatcaga
    13741 taacgaaaca aaagaatatc cttatgacaa attgattttg agttctggtg tcaaaccaaa
    13801 caatctgcca gtaccaggag cagatctaga aaatgtctac ttgatgcgtg gatacaattg
    13861 ggcaaccaaa atcaaagaaa aattaaatga tccggctatt aaaaaaatcg ctgtcgtagg
    13921 tgcgggttac attgggatcg aagcagcaga agcaagccga aaagctggga aagaagtcgt
    13981 attgcttgat gtgatcgata gacctttagg cacttattta gatgctgaga tgacagatat
    14041 tttagaacaa cacctaaatg aaaacggtat tgaagttttg acaaatgcaa aaatcgaagc
    14101 ttttactggt aacggaaaag ttgaggcaat caaaacaagt gaaaaagaaa tcccagcaga
    14161 tctagtcatt caagctgccg gtgtgaaggc aaatacagaa tggttaaaag ggatcgtcga
    14221 cttagatgaa cgtggctgga tccaaacaaa tgaatacttg caaacgaatc ttccagatgt
    14281 gtatgcagta ggagatgcta cacttgctta ttctatccca gcacgcaaaa aaatgccgat
    14341 cgctttagcg actgttgcac gtagagaagc acgttatgta gtaaaacatc tttttgaaga
    14401 agtaccaaac aaaccgttcg gcggagtcgt aggttcctct gcattaagtg tctttgacta
    14461 tcattttgca gcaagcggat tgaatagctt tacagcagca aaagcaggag tagatgtacg
    14521 agtgtcttat tatgaagata cgttacgacc aaaatatgta ccagaagaac aaggaaatcc
    14581 aaaagtgtcc atccaattat tctcggatcc aatgacccat caattgctag gaggagcagt
    14641 attatctacc tatgatgtta caggtcaagg aaatgtattg gcactagcga tccaaaacaa
    14701 actgacttta gaagaactag cagaagctga cttcttcttc cagccaggct ttgaccgaca
    14761 atggagcatg ttgaatctag ccgctcaaca agcgttagga gaagaaccat ttgtagaata
    14821 agaaaacgaa atatttgaaa agaggttgtg aaaaagctgc cttgcatcaa gtaataagaa
    14881 cagccaagta aaaacagctt cttatgtttt taacttggct ggacttatta ccgcagatgc
    14941 aagcttttga acaccgttta ttttcaaaaa agggactgtg acacgatttg tgtaccgacc
    15001 cccaaaagtt agaccaaaaa atctaacttt tggaggtcgg tattttttat ggttaaatat
    15061 acttttgaac ttaaattaaa gatcgttcat gattatttgg atggcaaagg cgattggacc
    15121 gtaaaaatcc agatcaagag attgaagcgg aaatgcttaa aattcgggag aagcacaagg
    15181 attatggctg tttacggatg accaatgaac taagaaaccg cggtttctct atcaataaaa
    15241 agaaagttca gcgcttgatc aagaagctag gaataaaagt aacggcatac acgcgtaaat
    15301 cacgtcgcta caattcttat c
//
LOCUS       DAYYKN010000061        15138 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000061 DAYYKN010000000
VERSION     DAYYKN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.061"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2377
                     /locus_tag="ACHBB7_001627"
     CDS             5..2377
                     /locus_tag="ACHBB7_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM9209583.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="ACHBB7_001628"
     CDS             2438..3898
                     /locus_tag="ACHBB7_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM9209584.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="ACHBB7_001629"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="ACHBB7_001629"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9209585.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="ACHBB7_001630"
     CDS             6129..6278
                     /locus_tag="ACHBB7_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209586.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="ACHBB7_001631"
     CDS             6353..6946
                     /locus_tag="ACHBB7_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM9209587.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="ACHBB7_001632"
     CDS             6959..7858
                     /locus_tag="ACHBB7_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM9209588.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="ACHBB7_001633"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="ACHBB7_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9209589.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="ACHBB7_001634"
     CDS             8724..8984
                     /locus_tag="ACHBB7_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209590.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="ACHBB7_001635"
     CDS             9429..9635
                     /locus_tag="ACHBB7_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM9209591.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9656..9886
                     /locus_tag="ACHBB7_001636"
     CDS             9656..9886
                     /locus_tag="ACHBB7_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209592.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="ACHBB7_001637"
     CDS             9902..10339
                     /locus_tag="ACHBB7_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209593.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="ACHBB7_001638"
     CDS             10332..11039
                     /locus_tag="ACHBB7_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209594.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11203..11403
                     /locus_tag="ACHBB7_001639"
     CDS             11203..11403
                     /locus_tag="ACHBB7_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209595.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11419..11598
                     /locus_tag="ACHBB7_001640"
     CDS             11419..11598
                     /locus_tag="ACHBB7_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209596.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="ACHBB7_001641"
     CDS             11923..12186
                     /locus_tag="ACHBB7_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM9209597.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="ACHBB7_001642"
     CDS             12180..12530
                     /locus_tag="ACHBB7_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9209598.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="ACHBB7_001643"
     CDS             complement(12554..13168)
                     /locus_tag="ACHBB7_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9209599.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13482..13904
                     /locus_tag="ACHBB7_001644"
     CDS             13482..13904
                     /locus_tag="ACHBB7_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209600.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13914..14117
                     /locus_tag="ACHBB7_001645"
     CDS             13914..14117
                     /locus_tag="ACHBB7_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209601.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14328..14912
                     /locus_tag="ACHBB7_001646"
     CDS             14328..14912
                     /locus_tag="ACHBB7_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9209602.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5570 a         2451 c         3061 g         4056 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga tggaggaaat agtatgtgtc acttttcttt
    13321 ttaaatggca atattttttg taaataaatg tcaatcaaga aaatttaaac gattatgaca
    13381 ttaaaaatat attttatgaa ttttctagta tataacttga tttatctgtt aagatttatt
    13441 aagaaaacgt tttcttattt ttgatttcga ggtgatagac aatgaaaaat aagtttattt
    13501 tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg
    13561 ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta actcctttag
    13621 ataggaacgg aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt
    13681 taatagtttc tccattactt gctcgagcag cttctaaaac gatacgagtg acaggaacga
    13741 tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa
    13801 aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa
    13861 acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag
    13921 aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac
    13981 cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa
    14041 aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat
    14101 atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta
    14161 attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc
    14221 aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta
    14281 tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat
    14341 acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat
    14401 ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat
    14461 tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc
    14521 gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg
    14581 tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc
    14641 tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat
    14701 ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat
    14761 tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga
    14821 ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg
    14881 aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt
    14941 gccatttatc tagaaaagtg acaatagtat gagaattttt tgtaagtgtc tactgataac
    15001 ttattctcaa atgttgtaag gattttattc atttgttaat attaaagaac ggaactccaa
    15061 aaaagtccgt actctaattt gcagtctctc actcttttag agtgagagtt tttattagat
    15121 tttcaaatta attagtaa
//
LOCUS       DAYYKN010000062        15029 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000062 DAYYKN010000000
VERSION     DAYYKN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.062"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..396)
                     /locus_tag="ACHBB7_001647"
     CDS             complement(67..396)
                     /locus_tag="ACHBB7_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM9209603.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            573..1301
                     /gene="sfsA"
                     /locus_tag="ACHBB7_001648"
     CDS             573..1301
                     /gene="sfsA"
                     /locus_tag="ACHBB7_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM9209604.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1317..2330
                     /locus_tag="ACHBB7_001649"
     CDS             1317..2330
                     /locus_tag="ACHBB7_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9209605.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2330..3607
                     /locus_tag="ACHBB7_001650"
     CDS             2330..3607
                     /locus_tag="ACHBB7_001650"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9209606.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3670..6372
                     /locus_tag="ACHBB7_001651"
     CDS             3670..6372
                     /locus_tag="ACHBB7_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9209607.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6524..6841
                     /locus_tag="ACHBB7_001652"
     CDS             6524..6841
                     /locus_tag="ACHBB7_001652"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9209608.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6871..7191
                     /locus_tag="ACHBB7_001653"
     CDS             6871..7191
                     /locus_tag="ACHBB7_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9209609.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7299..8759
                     /locus_tag="ACHBB7_001654"
     CDS             7299..8759
                     /locus_tag="ACHBB7_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9209610.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8827..9048
                     /locus_tag="ACHBB7_001655"
     CDS             8827..9048
                     /locus_tag="ACHBB7_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209611.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9079..9261
                     /locus_tag="ACHBB7_001656"
     CDS             9079..9261
                     /locus_tag="ACHBB7_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM9209612.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9261..9674
                     /locus_tag="ACHBB7_001657"
     CDS             9261..9674
                     /locus_tag="ACHBB7_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM9209613.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9797..10978
                     /locus_tag="ACHBB7_001658"
     CDS             9797..10978
                     /locus_tag="ACHBB7_001658"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9209614.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(11049..11213)
                     /locus_tag="ACHBB7_001659"
     CDS             complement(11049..11213)
                     /locus_tag="ACHBB7_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209615.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11367..11440
                     /locus_tag="ACHBB7_001660"
     tRNA            11367..11440
                     /locus_tag="ACHBB7_001660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11401..11403,aa:Arg,seq:cct)
     gene            complement(11509..12648)
                     /locus_tag="ACHBB7_001661"
     CDS             complement(11509..12648)
                     /locus_tag="ACHBB7_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9209616.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            12820..12954
                     /locus_tag="ACHBB7_001662"
     CDS             12820..12954
                     /locus_tag="ACHBB7_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209617.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(12947..13582)
                     /locus_tag="ACHBB7_001663"
     CDS             complement(12947..13582)
                     /locus_tag="ACHBB7_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209618.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(13695..14330)
                     /locus_tag="ACHBB7_001664"
     CDS             complement(13695..14330)
                     /locus_tag="ACHBB7_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM9209619.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(14364..14825)
                     /locus_tag="ACHBB7_001665"
     CDS             complement(14364..14825)
                     /locus_tag="ACHBB7_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM9209620.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(14955..>15029)
                     /locus_tag="ACHBB7_001666"
     CDS             complement(14955..>15029)
                     /locus_tag="ACHBB7_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFM9209621.1"
                     /translation="LRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT         4790 a         2525 c         2949 g         4765 t
ORIGIN      
        1 acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg
       61 agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt
      121 ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata
      181 ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc
      241 agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata
      301 ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga
      361 gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct
      421 ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa
      481 agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat
      541 aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt
      601 tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa
      661 ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag
      721 tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa
      781 aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg
      841 gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc
      901 gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa
      961 aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg
     1021 atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg
     1081 gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc
     1141 atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc
     1201 aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc
     1261 catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga
     1321 tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa
     1381 gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat
     1441 ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata
     1501 ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta
     1561 agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa
     1621 aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag
     1681 caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg
     1741 agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag
     1801 atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag
     1861 gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt
     1921 tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga
     1981 attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt
     2041 attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc
     2101 atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg
     2161 cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat
     2221 tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa
     2281 gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt
     2341 tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc
     2401 ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt
     2461 ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga
     2521 accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt
     2581 agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg
     2641 cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg
     2701 tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa
     2761 aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga
     2821 tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga
     2881 tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac
     2941 agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa
     3001 gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt
     3061 taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt
     3121 aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa
     3181 aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac
     3241 aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg
     3301 ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa
     3361 taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt
     3421 tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt
     3481 aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga
     3541 aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg
     3601 caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg
     3661 aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa
     3721 ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga
     3781 tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt
     3841 tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa
     3901 gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca
     3961 aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt
     4021 acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt
     4081 tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg
     4141 ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt
     4201 cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta
     4261 ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt
     4321 ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct
     4381 tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat
     4441 cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa
     4501 gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt
     4561 tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa
     4621 tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt
     4681 tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga
     4741 ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg
     4801 tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct
     4861 ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat
     4921 gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg
     4981 ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac
     5041 cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat
     5101 cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt
     5161 aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt
     5221 gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa
     5281 agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact
     5341 gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt
     5401 aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa
     5461 aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac
     5521 accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca
     5581 agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat
     5641 atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa
     5701 aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga
     5761 gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg
     5821 atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa
     5881 gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt
     5941 ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat
     6001 gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt
     6061 tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg
     6121 tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta
     6181 gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa
     6241 atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat
     6301 caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa
     6361 ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat
     6421 aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc
     6481 ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat
     6541 catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc
     6601 agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa
     6661 taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa
     6721 agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca
     6781 agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata
     6841 aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc
     6901 atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct
     6961 gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt
     7021 atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg
     7081 caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact
     7141 gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt
     7201 cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa
     7261 ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga
     7321 tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt
     7381 tgcgtgatgc ttttatcggc atactgcccg caacaatggc gggatcagtc gctgtaatga
     7441 tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag
     7501 cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct
     7561 ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg
     7621 cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc
     7681 aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg
     7741 tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag
     7801 ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta
     7861 tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga
     7921 tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag
     7981 cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg
     8041 ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta gggttatctc
     8101 aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta
     8161 ttcatggatc aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa
     8221 tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag
     8281 atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat
     8341 ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac
     8401 gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg
     8461 aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga
     8521 ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc
     8581 cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag
     8641 gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt
     8701 gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag
     8761 atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga
     8821 actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa
     8881 ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc
     8941 tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa
     9001 gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa
     9061 aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg
     9121 atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca
     9181 ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa
     9241 gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt
     9301 tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag
     9361 taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg
     9421 ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa
     9481 cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg
     9541 ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt
     9601 taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg
     9661 aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc
     9721 tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt
     9781 aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga
     9841 aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg
     9901 ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct
     9961 aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat
    10021 ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca
    10081 gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc
    10141 gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag
    10201 ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg
    10261 tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa
    10321 ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg
    10381 caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta
    10441 tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt
    10501 ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc
    10561 atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat
    10621 gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc
    10681 atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg
    10741 gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga
    10801 acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg
    10861 gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga
    10921 agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact atttttgata
    10981 aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa
    11041 ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt
    11101 ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt
    11161 ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag
    11221 gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa
    11281 cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa
    11341 caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct
    11401 cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tataaataac ccctgtatcc
    11461 tttgcggtac aggggtttgt tttgtatttg tgccaaaaat gtgccaaact agttatcatt
    11521 tttatataaa ctatcgaaca cagtattgat tttttcttta tcctgatctt caagctctcg
    11581 aacaatatga agataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga
    11641 tacgctaagt atatttactc cttgataaag taaaatagat gcatgtgtat gccttagtcc
    11701 atgcaaagta aattgctttt caatccctaa ttcttttaat tttttgcgca aatatttgct
    11761 gacggcgttt gatgaaacta atccattttt taagttgaaa aaaacaaaat tatgagggtt
    11821 tttgatttca aaattctcga ataattcttt ttgattgatt ttaaactttt ttaagagatc
    11881 aatcgtatgt ttatcaatgg aaattttcct atttgaagtt tcgttttttg tttttcccca
    11941 ttcatttaat ttataattcc atgttttatt aatagtgatg atttgatctt cgaaatcgat
    12001 atcttcccat gttaatccta gaagttcagc aaatcgcatt ccagtggcac cagcaactaa
    12061 cacgagcata ggagaagagt actgcgcaga aaggttttct tctacgagtt tcattaaaga
    12121 tttaaattga tcataatcta aatatttatc ttcttttggc ttcaatgaat cttttccttt
    12181 gattactgct tttctagttg gatcaaacgg aattaatcct tcttctacgg catctttcaa
    12241 cgacgctcta atatggttat taaacttaat aacggtagat tttacatggt ctttagcata
    12301 cttatttaaa aagcgttgat atcccactct atctaaatca gaaatcaata ccgctggcat
    12361 atatttcttt atgttcatta acgtatcttc gtattttcta taagtgatag gagatactgt
    12421 tccttcttta taaagttgca tccaatcctc aaagtaatct gataataata gatttttgcg
    12481 atccccttta aggcctttgt tcaagttata ttctaattcg ttggcggcat cttttgcttc
    12541 tccttttgtc ttaaatcctg attttcttat cttcgtgtat tttccgtcat cttttttgta
    12601 tgaaatttcg tattgccaac tatttccacg tttgactaat cttgccataa ttgaatcagc
    12661 tctctttctc tgatacaata ggaactataa agaagcccat tgtttgggtt ttattatttt
    12721 ttgcacatcc tttttgcttg ccggcgcaag gatgtgctat ttgtcgtttt aatggtataa
    12781 ttggatgtgt attagatcat ccaatcagag gtgtttatta tgattaaagt gatttttaaa
    12841 agaataactg aacttccaga gtttagatta tttgttgatt attggtatgt ctggtttttt
    12901 attgttgcat tgctgatagg tctagatgtc atattagaaa gaaaacttaa gtaagagttg
    12961 agttaataaa ttgaattcct aaatgagata ttttgtagcc catattttgt ttgccgacat
    13021 cacctaatac tatggagcta atcgtttttt ctaacagacc aaaatcagat aatctattta
    13081 agctaccatc tattagctct tgtgaatagc cagacatgct gatatttcca gtgaaatttt
    13141 cgtttccacg actcattaag catgctaaca attgaatctc aatcgatgtt aattgattca
    13201 gtacaagaag ataatactcc tcgttatccc atgaagcgtt gttaattcga agtaaacaat
    13261 tcttataaag atttttatac atatctattt tattttgtgc ctttgatttt tctacctcgt
    13321 catttatccc ttcgaatatt ccaatgagtt ggtccttgtt ctctaagttg aaaacatctt
    13381 ctggaatttg atcgcgaatg ttttctaatg attcgtttaa atttttgtag aatttctcga
    13441 tgcgtttgaa acgcttctcg tttttagacc caaaataaag tgactgcaca acgccaccaa
    13501 tataaggaat tgctccaata ccgccttcaa ctattaattc aagatattct tttttggtta
    13561 gtttttcatt gctattatcc atagttacct cctattttgt catttccttt acaagcattg
    13621 tgactcctga tggcactctt ttgtataggt tttattattg tgcacaatct aactcttagt
    13681 ggggcgtggt tcatttattt tttacttact ttactattaa tcatatccca ttgagcatag
    13741 gatttttcta tgttcgatcc tcttgaacta atttctcctg taaaagttac tttatcgccc
    13801 tttgtaaagg tatcagcatt gtttaagtcc ttcgcaaaaa ttacatatga aactttaggt
    13861 gttgcagaaa tatcattcca acttttatca tcgtactctt tgtctgctat aatggctatt
    13921 ctagtagtca ttgattcgat aacagttcct tcaaaagtat atgttttacc actaatatat
    13981 ttagtaaaag ctgtattttg ctcagtagct ggagtaatag aataataact atctaagaaa
    14041 gcaccccagt catgattatt tgtctctaaa aattcttcaa attgagttag gcttttattg
    14101 ctgctgttag atgtagtagt ttgagtatca gatgatgagc tttcctgttt gttattgtca
    14161 tttgcctcgt ttgatgagga aagcgagaaa cctacggtag taaaaataat cccgataata
    14221 aggagaatca taggtgtttt cttatttgtt tttttgataa atcttacaat taacatgatt
    14281 aaacctataa taaataaaac aataccagta aaaccaaata taccgtccat taatagatcc
    14341 tctttctctt ttgatattga atattataaa ccaagcaatt cttttttctt aaaatcgaat
    14401 tcttcttgat taataatccc ttcatctaac aactctttat actttttaat ttcatctgca
    14461 cttgaaattt gtgtttcttt ggaattatta tctaatatag attgttgctt ctctatatat
    14521 ttttttaaat tttccatttc attccaatat ttttttgtaa acataacggt gttttcatct
    14581 ttcgtggccg ctaatatacc tccgcgactt tcgtttccgc ctagcaaggt aaattgtata
    14641 taaccattac tcatacctgg tttttttaat tgtacggctg atatattttt aaaggggata
    14701 gttttttctc ctttaatacc ttgattaact aaatttataa atccttttct aataattgaa
    14761 atagaatcat cagttacttt aatcaaggtt tttcctggag attttattaa aatctctttt
    14821 tccatttttt cttcctcatt tctgtgatat gatttttttt gtagaagctc atatatgagg
    14881 aaagagcccg tgttgcagca cgggcttttt taatgttttg ctgaaatcgg tttttttaat
    14941 aattcttgat gtattcatac atattgcctt gcgtaagaat attttttctt aaaatagcat
    15001 tggcagacag cataacaagt gtatctgag
//
LOCUS       DAYYKN010000063        14852 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000063 DAYYKN010000000
VERSION     DAYYKN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.063"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..493)
                     /locus_tag="ACHBB7_001667"
     CDS             complement(41..493)
                     /locus_tag="ACHBB7_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9209622.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(958..1326)
                     /locus_tag="ACHBB7_001668"
     CDS             complement(958..1326)
                     /locus_tag="ACHBB7_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM9209623.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            1476..1958
                     /locus_tag="ACHBB7_001669"
     CDS             1476..1958
                     /locus_tag="ACHBB7_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM9209624.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1995..3005
                     /locus_tag="ACHBB7_001670"
     CDS             1995..3005
                     /locus_tag="ACHBB7_001670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM9209625.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(3120..3833)
                     /locus_tag="ACHBB7_001671"
     CDS             complement(3120..3833)
                     /locus_tag="ACHBB7_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209626.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(4006..4613)
                     /locus_tag="ACHBB7_001672"
                     /pseudo
     CDS             complement(4006..4613)
                     /locus_tag="ACHBB7_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            5097..5324
                     /locus_tag="ACHBB7_001673"
     CDS             5097..5324
                     /locus_tag="ACHBB7_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM9209627.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            5340..6506
                     /gene="hemW"
                     /locus_tag="ACHBB7_001674"
     CDS             5340..6506
                     /gene="hemW"
                     /locus_tag="ACHBB7_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM9209628.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6740..7783
                     /gene="hrcA"
                     /locus_tag="ACHBB7_001675"
     CDS             6740..7783
                     /gene="hrcA"
                     /locus_tag="ACHBB7_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM9209629.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7877..8440
                     /gene="grpE"
                     /locus_tag="ACHBB7_001676"
     CDS             7877..8440
                     /gene="grpE"
                     /locus_tag="ACHBB7_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM9209630.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            8491..10320
                     /gene="dnaK"
                     /locus_tag="ACHBB7_001677"
     CDS             8491..10320
                     /gene="dnaK"
                     /locus_tag="ACHBB7_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM9209631.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            10471..11637
                     /gene="dnaJ"
                     /locus_tag="ACHBB7_001678"
     CDS             10471..11637
                     /gene="dnaJ"
                     /locus_tag="ACHBB7_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM9209632.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11719..12348)
                     /locus_tag="ACHBB7_001679"
     CDS             complement(11719..12348)
                     /locus_tag="ACHBB7_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9209633.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            12360..12533
                     /locus_tag="ACHBB7_001680"
     CDS             12360..12533
                     /locus_tag="ACHBB7_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209634.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(12578..13315)
                     /locus_tag="ACHBB7_001681"
     CDS             complement(12578..13315)
                     /locus_tag="ACHBB7_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9209635.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(13317..14819)
                     /locus_tag="ACHBB7_001682"
     CDS             complement(13317..14819)
                     /locus_tag="ACHBB7_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM9209636.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
BASE COUNT         4876 a         2599 c         3014 g         4363 t
ORIGIN      
        1 atttccaaca tctctggaat ttctagcgct ttttaattgt ttaagataca agggaaatat
       61 ctgtctggat caataatttt tcattgatcc attgactaaa gtttacgaca taattgaagt
      121 taccaccgat tagtatctcc gcatgattgt ctttataaag atcgtattgt aaaagacggt
      181 cttttttatt tgcgtaaaca tcgttatggt actggactaa ttgttctcgg aattgattga
      241 ttgctgtaaa atcacgatat tgaaggttga aacgcatcgt gattggtgca tttagcatga
      301 atcgctgcat ttcttttgct gcttcctctg cttgatctcc gtataggaac tcaaaaagat
      361 tacgttcaga aaatttccat tgcccgccaa cctttgttcc ttttagtttt ccttcatgaa
      421 gataatttct gatagaacgt gtagtaacac ctaattgttc ggctaattca tttactgagt
      481 aaaaatcttt caatgttcac cctcctatgt gaatggttct actgacttag ttgatatttg
      541 cttaatcacg aaatgtagaa agaacgattt taattaaggt cattgtaaaa atcaagtcag
      601 aaaattctaa cttttatttc tctaaagaaa ttcgttctct cctaaagtga tattatattt
      661 aaatatacct gaaaataatg ggcagtatta tgaaagatca ctgatatttt ctcgtatttt
      721 ttccttattc aaaagttttg caggaaaatt gctgaaaaaa taattttact aagtagaaaa
      781 ttcaaagcca ttcaatcagt gatttttttc agcacaagaa agactcctta taaaaatagc
      841 agatactttg tcaattaagt gtttaaccag ggatgataaa aaggatagga ttgaacaaaa
      901 cctgtataag gtgatgaata gtttcctcgc cagtaaaagg tttattcgta taaagcttta
      961 tcgccaaaat ttatcatgac cttttttatc ttcaatatta aatgaaatca attgttcaat
     1021 caattcccag ttgattacac ttttccaagg aactttgatg acgttatccg tatatacgta
     1081 gcctgcttct tggatatttt tttcaagggc acgaatcgct gctttttccg gtgcgataga
     1141 aaaatgattt ttagcaaaac taaatgcaat aataaaggta ccatggtcag taaacatagg
     1201 ttggttccat tttacagttg tgtctagctg gggatacgtt tctgttatct tagcaaaaag
     1261 gtctttgatc ttttgttgat gttctttttc tttgatttga ttgatgatcg gctgaaattt
     1321 ttccattcta aaacgcctcc attaagtggt ttgatttatt ttaccagaaa aacaatagtt
     1381 atgtataaaa aacgaacaaa acacaagtaa tgttgtaacc gctatattta tatgataaga
     1441 tgtctcaaat aataaaatag aaaagagaca taaaaatgat tgacttggca aagaaattct
     1501 tacacgaaaa tatatatttt gagttgtatc atcatcgagc catttataca aatgaagatg
     1561 ctttggtaat aaagaaagaa caaggattta caggtacaga gaccaaaagc ttattcttaa
     1621 aagataaaaa agataataac tacgtatatc tgacttttac tacgaaacaa acggatttca
     1681 aagcattagg gaagatcgtc ggtaaaaggc tttctattgt ttcatcagaa cgcatggaag
     1741 aacaaacggg acaaaagcag ggcgctgttt ctccgtttgg ttatgaaaaa cagattccga
     1801 ttattattga tgaagagctc ttatcaaatg aaaaacttgt ttttgcacca ggtagaccag
     1861 atcaaacgat ggtcatccaa acggttgact taaacaaaat catccagcta cttgagatgc
     1921 cgatattttt gatgccggca aacgaaaaaa acaattaacc aagtaaaata gtgcatactt
     1981 ggaggtgaag caacatgata agttatccat tattagaaag aaatgcaacg attggggtta
     2041 ctgcaccatc ctcaggcgta tcagaggaac accattcgtt actcgaatca gctttaaaga
     2101 gaatggaaga aaaatctttc caagtagtta ctggaaagac gctttggacg caagataagg
     2161 aaaaatcagg tccagcaaga aaacgagcag atgaattcaa tcaaatgatg caagatgaaa
     2221 atatccaaat gatctttccg acttggggag gagaattatt gattgagatt ctcgattttg
     2281 tcgattttga tgctctacaa ccaaaatgga tagtaggtta ttcagatatt agtacgttgc
     2341 tacttgctgt cacattaaaa acagggatag ccactgcaca tggaactaac cttattgatt
     2401 taagaggaga gtacttggat gaaacgacag caatgtggga aagtgtactg aaaacaagag
     2461 aaggagaatc gattctacag caatcttctg aaaagtatca aaaagtgtgg ccccatgagc
     2521 attcatcatc ttctgttttc catctcactg aaccaacaaa ttggcaatca gtcagcggag
     2581 atgcagtaag gatgaaggga cgattattag gtggttgtat tgatacgatc aggcatttaa
     2641 tcggtactcc ttatggagat gtccaatctt ttaaaagaaa gtataatgat ggaaagccga
     2701 tcttgtggtt tctagaaaac tgtgagctat cgtcagttga tttaagaaga tcacttgttc
     2761 agatgaaact ggcaggctgg tttgatggca gctcggggat catgttcggt agaagtgcgg
     2821 cagaggattt gttagattat aaaatgaagg atgtatacga agatctgtca aaggaattaa
     2881 aactaccgat cttgtatgat attgattgtg gtcatgtacc gccacaactg acattgatta
     2941 atggtgccta tgcagaagta gcggtagaag atagaaaagc gactgttttg caaacgttta
     3001 tttaaaaaat tttttcctgc cttggagtct atcaaagacc aataaatcta aatagaaaag
     3061 aaagtgaaag aagctgacaa aatgctgtca gcttcttttt gtataacata ttagaaagtc
     3121 taattatcta taactgtcac atcgattaac tgtttcacgt cattgggatt tcctgtagga
     3181 atagctacta cagatgcatt atcaatgatt tcttgagttg tttctctaat gaacgctgga
     3241 taagatgttt gatctgcagg catttgccaa tgtgaaggaa ctggataact agtgattgtc
     3301 ccgtcatggt tacttgaata cactaaaact ccctggtaac tgtatttccc agtgagcata
     3361 accacatctt tcggatagac tgcactccct gcatcataag gggcgatagg tgttcctgca
     3421 ggataatgaa taacatgtaa ttcgaaatca cccgatgtat ctctaaaagt attacctcta
     3481 atagctagcc aaacacgtgc gtactcaatt tgtagatcag aatatcctgc taacggatct
     3541 attgactgag aagactgtga ggaggttgtg tctattgttg aagaagaaat atttgtctca
     3601 actgtactac tagatgtaga agaagtattg ggctctgaga ctttactgct tgttgaagat
     3661 gtattcgttt cagtagaagc tgatttctga gtacttgatt tttcagctga actggtttgt
     3721 ctgattgaac ttttgtctgt ggtcgcagat ggatcactat tttctttact aggagacgta
     3781 catcctacaa ggaataaaga gaccaacaat aaaataaaac ctaacttttt catataaaat
     3841 acctcattat ttttttaata ccagtatagc ataatcctga atttttgaat ccgtaaaacc
     3901 ttgtctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa agtgtgacaa
     3961 ataatcatca gtaaaattac ttgtcacact attgataagg agtatttaat tgaagagaat
     4021 gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag tttgtataga
     4081 cgaatcgttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag aatcaagtct
     4141 gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt cacagcttgt
     4201 tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg aaattgcgca
     4261 tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc aactgcccca
     4321 caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg atataaatga
     4381 tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac tttttcaggt
     4441 atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt gacctctgat
     4501 tggatagaag cagttttgtt ttgccatttc tcattttggt ttagtgtgct agtaatataa
     4561 gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt cattttcaag
     4621 ctctccttta ctatctataa caaggacaac agttttaacc tacagacatt cattaaaatt
     4681 gggtagcgac agcgaaaaca acaattctta agttttagac agagtataca gatagtcaac
     4741 ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac cagcattata
     4801 acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt acaatattaa
     4861 aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat cgggcactca
     4921 gacatgaaaa caaccctaga gatatataac taggtaacca gtttaatgaa agaaaaaatc
     4981 atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac gtttagtatg
     5041 cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag gaatctgtga
     5101 aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc tttgcgatta
     5161 ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag caagcatttt
     5221 tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg gtgcttgaac
     5281 gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa agaatttcaa
     5341 tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat atcccatttt
     5401 gcgagcatat ctgctattac tgtgatttta ataaagtttt tttagaaggg cagccagtag
     5461 atgagtatgt ggaaatgctg ctgaatgaaa tgaaaataat gctagaaagt catccgacag
     5521 cgaatttaga aacactttac gtagggggcg ggacaccaac ttctttatct gctaaacaac
     5581 ttgatcgact gttatcaggt gctagagaga tcctgccttt tcaaaatggt aatgagttta
     5641 ctgtagaagc gaatccaggt gatttgaccc gtgaaaaact tcaagtaatg aaaaactatg
     5701 gcgtcaatcg tttatccatg ggagtgcaga catttgataa tcgcttattg aaaaaaattg
     5761 gacgtaaaca caccgcagaa gatgtgtatc aaactatgca atttttagaa gcagaaaatt
     5821 ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta gaaggatatc
     5881 tggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg tactcattga
     5941 tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc gaactgcctg
     6001 atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa aagcatggac
     6061 gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa cataacctca
     6121 tgtactggaa caatgcccac tattttgggt ttggagctgg tgctagcggt tatcttggag
     6181 atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta cgagcaaatg
     6241 aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa gaagaaatgt
     6301 tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat cgctatcaaa
     6361 aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa gggttacttg
     6421 ttaatgaaga agaccgtgtt tatttgacac caagagggac ctttttgggg aacaatgtgt
     6481 ttgagcgctt tttgtttgaa aaatagttta aagaatattt gttttttatc tgaatttaaa
     6541 taaaaaaagt tcaaaaatac aataaaggac tattgaaacg ttgatataac tggatttaca
     6601 tcaattattt agaattagca ctcttgtgtt tagagtgcta aaaaatggag tctttctctt
     6661 gacaggagaa gggcttcttg ctatatttat aagtggatta gcacttagaa tatatgagtg
     6721 ctaatgagag gtgaggaaga tgttaacaaa gcgacaaaac gacattttac gtctgatcat
     6781 ccaacactat acgagtagtg gcgtgcccgt tggttccaaa acattgatgt ctgagggagt
     6841 caaggccagc tcagcaacga ttcgcaatga tatgaaagca ttagaagatg aaggattgct
     6901 gttgaagact cactcttctt ctgggcggat tccatctgct ttaggctatc gttattacgt
     6961 ggatcatttg ttaaaacctg ctcgcgtagc agaagatgat ttgctgctga ttcgtcagtc
     7021 tctaggcaga gaatttcatg aaatcaatga gattattaag caatctgctg agatattatc
     7081 tgagctgaca agctatacga ctttttcatt gggacctgaa ataaaagacc gccgactgac
     7141 tggctttcgt atcgtaccct tgaataatcg acagattatt gctatcgtcg taacagatat
     7201 ggggaatgta gaaagccaag tgttcactct tcccgaatat atgggaagtc aggaactaga
     7261 aaaaatggta cagattgtaa acgaccgtct agtcggagat cctctgatga cggtgtacca
     7321 taaacttcgt acggagattc cgatgattct tcataaatat ttccaaacga cagaagggat
     7381 gtctcagtta tttgacaccg ttcttggtca tgtttttgaa gataaggttt atgtcagtgg
     7441 acaaatgaat ctcttggatt atgagccccg tcaagatctt gatcagttca aatcgatgtt
     7501 ttcttttatg aaagactcag acggacttac acaaatgatc actccgatgg atagcaatat
     7561 tcatatcaag attggtgctg aattaggcaa tgatttattg cagaatatga gtatgattca
     7621 agcaagttac gagatagatg gtcatggaag aggtacaata gccttgttag ggcctactag
     7681 tatgccttat tctcggatgt tcggtctagt cgatgtttat cgaagagaac tagcagctaa
     7741 acttgctgat tactaccgtt cgcttgactt gtcaaactcg taatgtgaat aacgaagcta
     7801 tttgcagctg atcgttctct ctttcttagt aagagcaatc aaacagctga ctgaataatg
     7861 aaaggaagac tgtatcgtga aagaaaatca agaagaacta gataaagaaa tgacagatgc
     7921 tcagccagaa ccggaaatcg atgtcgaagc tgcagaagat tcgggtattt cagaagctga
     7981 agcggaagaa ttcgaaacag cgaaattaaa agctgagtta gaagaaatgg aagataaata
     8041 cttgcgggct cgtgctgaaa tcgcaaatat ggcaaatcga ggaaaaaatg agcgtgagca
     8101 attacaaaaa taccgttcac aagatttagc caaaaaatta cttccatcaa ttgacaacct
     8161 ggaacgtgca ttagccacag aagtgtcaga tgatcaaggg gcaggtttga aaaaaggcgt
     8221 ggaaatggtc ttggaaagtt tgcgtaacgc actggaagaa gaaggaattg aaaaaatccc
     8281 tgctaaagga gaagcctttg atcctaatct tcatcaagca gtacaaactg ttccagcgac
     8341 tgaagataca ccagccgata cgattgtaga agtactacaa gaaggctaca aattacatga
     8401 tcgcgtgtta agaccaacca tggtcattgt tgcacaataa ttgtgcggat ataaaactta
     8461 cactttcaat taaagaagga gacgttaatt atgagtaaaa ttatcggtat tgacttaggt
     8521 acgactaact ctgcagtagc agtattggaa ggaaacgaag caaaaatcat cgctaaccca
     8581 gaaggaaacc gtacaacacc ttctgttgta tcattcaaaa atggagaaat ccaagtaggg
     8641 gaagttgcaa aacgtcaagc tgtgacaaac ccaaatacga tttcatcaat caaacgccat
     8701 atcggtgaag caggttacaa agtagatgta gaaggtaaat catatacacc acaagaaatc
     8761 tcagcaatga tccttcaata tatcaaagga ttcgcagaag actatttagg tgaaaaagtc
     8821 gaaaaagcag ttatcactgt gccagcttac tttaatgatg cacaacgtca agcaacaaaa
     8881 gatgctggta agatcgctgg tctagaagta gaacgtatcg tcaatgaacc aactgcagct
     8941 gctcttgcct atggtttaga taaaacagat cgcgacgaaa aaatcttagt attcgacctt
     9001 ggtggcggta cttttgacgt atctatcctt gaactaggag acggtgtctt tgatgtattg
     9061 tcaacagctg gcgacaacca tctaggtggg gacgactttg ataacaaaat catcgactac
     9121 atggtagcag aattcaaaaa agaaaacagc attgacttgt ctcaagacaa aatggcttta
     9181 caacgcttga aagacgctgc tgaaaaagcg aaaaaagatt tatctggcgt atcaagcaca
     9241 caaatcagct tgccatttat cacagcagga gaatctggac cattgcattt ggaaatgaca
     9301 ttgacacgtg caaaatttga tgaattaaca gctgacttag tagaacgtac aaaagtacca
     9361 gtacgtcaag cgctaaaaga tgcaggttta tcacaatcag aaatcgacga agttatttta
     9421 gtcggtggat ctactcgtat ccctgcagtt gtagaagcag tacgtaaaga aacaggaaaa
     9481 gaaccaaaca aatcagttaa ccctgatgaa gtagttgcta tgggtgctgc tatccaaggt
     9541 ggcgtgatca ctggtgatgt gaaagatgtt gttttacttg acgttacacc attgtcatta
     9601 ggtatcgaaa caatgggtgg agtgttcact aaattgattg accgcaatac aacgattcca
     9661 acaagtaaat cacaagtatt ctcaactgcg gcagataatc aaccagcagt agacatccat
     9721 gtattacaag gtgaacgccc aatggctgct gacaacaaaa ctctaggaag attccaattg
     9781 acagatattc cagcagctcc tcgtggtatt ccacaaatcg aagtaacatt cgatattgat
     9841 aaaaacggga ttgtaaatgt atctgcgaaa gatctaggta cacaaaaaga acaaaaaatc
     9901 acgatcaaat cttcttcagg tctaacagac gaagaaatcg aacgtatggt aaaagatgca
     9961 gaagcaaatg cagaagcaga taaagctcgt aaagaggaag ttgacctacg taatgatgta
    10021 gatgcattgc tattctctgt ggacaaaaca ttgaaagaat tagaaggcaa agtggacgaa
    10081 gaagaagtga aaaaagcaga aacagctcgc gatgaattga aagccgctgt tgaagcaaac
    10141 aacatcgaag aaatgaaaac taaacgcgat gcattgaacg aaatcgttca aaacttgact
    10201 gttaaactat acgaacaagc ggcacaacaa caagctcaag aaaacccaga agcagctcaa
    10261 ggcggagcag atgatgtcgt tgatgctgat tttgaagaag tcgatggcga cgataaataa
    10321 tcagttcact gaatttcgag agaaggccaa agttgctact ttggcttttt cttttgaaaa
    10381 atagtttgaa gtatcggttt ttgtttcaaa caaaatatga tatgataaca agcgacgtga
    10441 aaatgatgag aaccaatagg aggaaagcct atggcaacaa aacgtgatta ttatgaagtc
    10501 ttagggctct caaaaggagc atcagaagat gaaataaaga aagcttatcg taagctttcg
    10561 aaaaaatacc atcctgacat caataaagaa cctgatgcgg aagaaaaatt caaagaagta
    10621 tcagaagcat atgagatttt aagtgacccg caaaaacgcg ctgcatatga ccaatacggg
    10681 catgcaggca cagatccaaa ttacggtgct ggtggagctg gtggcggatt tggtggattc
    10741 ggcggcttct ctggtggcgg cttcggtggt tttgaagata tcttcgactc tttctttgga
    10801 ggaggcggtc ggtcagtcga tccaaatgcg cctcgtcaag gtgctgattt gcaatatacg
    10861 gtcgacttgt cttttgaaga agcgattttt ggtgtagaaa aagaaatcaa gtataatcgg
    10921 gaagaaatct gtcatacttg tggtgggaac ggtgcaaaac caggcacaca accaactact
    10981 tgtcataaat gtcatggtgc aggtacgatt aatgtagaac gccaaacacc acttggacga
    11041 gtgatgagcc gtcaaacttg tgatgtttgt catgggacag ggaaagaaat caaagaacct
    11101 tgtccaacct gtcatggctc aggacatgag aagaaagcac attcagttaa agtgaatgta
    11161 ccagctggtg tagaagaagg acaacaaatg cgtttagctg gtcaaggaga agctggagaa
    11221 aacggcggac catttggtga tttgtatgtg gtcttccgtg tagaagaaag tgatatcttt
    11281 gatcgtgaag gttctgaaat ttactatgaa ttgcctttaa gttttgtaca agcagctttg
    11341 ggggatgaag tccaagtacc aactgtccat ggagatgtga aattgaagat ccctgctgga
    11401 acacagacag gtacgaactt ccgtttacgt ggaaaaggcg ctccgaagtt acgtggtggt
    11461 gcaacaggcg accaacatgt caaagtaaaa ctgatcacac cgaaaaatct aaatgaacaa
    11521 caaagagaag cattacgtgc attcgctgaa gctggcggca tcaaagttga agaacagcaa
    11581 gaagacggtt tcttcgataa gtttaaagat gcttttggcg ggaaaaagcg gaagtagttt
    11641 ttctagtaaa aacgctaagt cattaataat taaaaaccag acgaacaatc atatgttcgc
    11701 ctggtttttt tgtagttgtc atgtttgtac caacaaaaaa gccagcttta gaatatgata
    11761 atcgtcatag tttctagggg tataaccagt gagagaatgg aacttgttca atcgatattg
    11821 caaggtgttt ttatgaataa ataattcttc tgagcaattt ttgatgcttc cgttattttt
    11881 actgaagcat gccaaagtct tcttgaacaa atgtacttct tcttctgtca ggcttttcaa
    11941 aatacgtttt acatataatg ttcgttgatc tgacgggatt gctggtaaaa cgatcccgag
    12001 gtctaaatct tcaaaaaagc ggatctcgtt cacagattga tggaaagcta tccattcgca
    12061 aacttgtttg gcatgaagga aagattgcgg tgtctcttgc tctgtataac aaagcgaccc
    12121 aattccaaag tttagctgta actgatacat tttattaaac tttgtctgga ttttatcaag
    12181 caatgatagc aatgaggctc tggaaatccg attgactagg ataatgattt gttgtctatg
    12241 tacagtgagc aaagcctgtc cgtaataaga gaagaccctt tcgagcaact ccaataaagg
    12301 attgatccgt tcttgacaca attgctgatt gtcagaaagt tgtcccacaa ctacataata
    12361 tggagaagaa aaatcaagct caattaagtg agggtggtat cgactatgta cttgatatca
    12421 tcaatgaacc gatggtactt tcttatgcga tggaacaggt agagcgattg cttgtttgtg
    12481 caggaaaaca agcgatgcgg ctgatacgat taggtgagaa aataagaaga taaaaataca
    12541 cggtcccttc atggcgaccg tgcatttttg gtttttatta aatatttaat actttattca
    12601 agaaatcttt tgtccgttca ttttgcggat tttcaaagat ttgttgaggc gttccatctt
    12661 ctaaaaagtt tccgtcgtca atgaacatga cgcgattggc cacttcttta gcgaacccca
    12721 tttcgtgcgt aacgataacc atcgtcatac cttgttctgc taactttttc atgacgttca
    12781 agacatcacc gaccatttca ggatcgagtg cagaagtagg ctcatcaaaa agcatgatgt
    12841 cagggttcat cgctagagca cgagcaatag ccacacgctg tttttgtcca ccagaaagca
    12901 tttcaggata catatctttt ttatcttcta gaccaactgt ctgcagtaat ttgattgctt
    12961 ttttctctgc ttcttctcta gattctttct tcaggtccat cggtgctaaa atgatgtttt
    13021 ccaaaataga aagatgaggg aataggttaa agtgttggaa taccattccg atatgttgtc
    13081 tcaccaagtt gatatctgtg cttttatcca tcaagtgggc gccgtcaata ataatttcac
    13141 cgtcagaagg ttcttctaaa cgattcaagc aacgcaagaa tgtacttttt cctgaacctg
    13201 aagggccaat gatacagacg acatctcctt cgttgatgga tacattgatg tcgtttaata
    13261 ctgtgttatc gccgtatttt ttgactaaat gctcaactaa gattttttct gccatcttac
    13321 ttcaccttct tttctaaaac ttttgctaat tttgtcaatg ctgtgatcaa aatcaagtac
    13381 atgatcgcaa tgatcaagta aacatacgta ctttgtgttg tacgtgcaac aatgatctta
    13441 ccagtttgca gcaattcgac tacaccgatt gcggaaatga tcgttgtgtc ttttaatgag
    13501 ataacgaact gattgacgaa tgatgggatc atgatgcgta tcgcttgcgg caaaatgatt
    13561 ttttgcattg tccgattata agaaagacct aaacttctgg aagcttccat ttgtccgaca
    13621 ggaacggcgt tgatcccgcc tcgaacaatc tctgcgatat aagcacttgc gttaagagtc
    13681 aaggtgataa caccagcaag gaagtcagga atcgtaaatc caacgatacc aggcaaccca
    13741 aagaaaataa agaacgcaag aaccatcatt ggaattcctc ggatgatatc gacataaata
    13801 ctagcaattg tgcgaagagc gcgtattggt gctacactga ataatccaaa aatcacaccg
    13861 attactatag ccaaagcaaa ggagatcaaa gttaggagaa ttgttctcca aagtcctttc
    13921 aacaatgctt gatagttgtt ttggataagt ccaagaaatg ttgattcatc agcagtggaa
    13981 gactcactgg aagtttctga accatccgct acatattctt taataatctt atcgtattcg
    14041 cctgttcttt ttaattcttt caatccttca ttgaacatat taagaagctc aggattttgt
    14101 ccttttttaa cggcaaatgc atattttccg ccagattctt ttttgatcgg tgtttccaat
    14161 tcttgtcctt gtttgatcgc ataaccgatt accggataat catccatcat tgcatcgatt
    14221 gcgccaactt ttaacccgtc gtacaattga tcagcagcat caaaatattt gatagaatag
    14281 ccatattcag attcatggtc gcttaaaaat tcagcacttt cggtaccaac ttttgctccg
    14341 actgtttttc ctttcaaatc ttcataagac ttgatcgaag tatctccttt tttgacagct
    14401 aattggatcc cgctatcaaa gtaagaatca gaaaaatcat agcttttctt tcgttcatct
    14461 gttacagtca ttccggcaat cattccgtct gcttgtcctg attccaaagc ttgcaaagca
    14521 gcagagaaac cacggtagtc cattcggatg ttaaatcctt gcagtttggc gatgttttcc
    14581 atcaatttta catcgatgcc ttcgtactcg ccctttgcgt tttgaaattc aaatggagca
    14641 aaagcagagt cactggcaat tacgtatacg tcttttttgg gctctatttt ttgcattgtc
    14701 tcgcttgtgc ttgactctgc cgttgtagtg ttgtcagctt gtgcaacatg accaaacgca
    14761 agtgtcacaa gtcctgtcat taagaaaaca aatgagtgaa ataaatgctt tcttttcata
    14821 atcaacctcc taaatttcca atattttatt at
//
LOCUS       DAYYKN010000064        14815 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000064 DAYYKN010000000
VERSION     DAYYKN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.064"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHBB7_001683"
     CDS             complement(296..1024)
                     /locus_tag="ACHBB7_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9209637.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHBB7_001684"
     CDS             complement(1002..2576)
                     /locus_tag="ACHBB7_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9209638.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHBB7_001685"
     CDS             2805..3743
                     /locus_tag="ACHBB7_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9209639.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHBB7_001686"
     CDS             3755..4666
                     /locus_tag="ACHBB7_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9209640.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHBB7_001687"
     CDS             4690..6162
                     /locus_tag="ACHBB7_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9209641.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHBB7_001688"
     CDS             6213..6824
                     /locus_tag="ACHBB7_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9209642.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACHBB7_001689"
     CDS             6854..8767
                     /locus_tag="ACHBB7_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM9209643.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACHBB7_001690"
     CDS             8895..11876
                     /locus_tag="ACHBB7_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM9209644.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACHBB7_001691"
     CDS             11873..13534
                     /locus_tag="ACHBB7_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM9209645.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACHBB7_001692"
     CDS             13574..14506
                     /locus_tag="ACHBB7_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM9209646.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAYYKN010000065        14789 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000065 DAYYKN010000000
VERSION     DAYYKN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.065"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            124..1083
                     /locus_tag="ACHBB7_001693"
     CDS             124..1083
                     /locus_tag="ACHBB7_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM9209647.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMRGKRKPNGHQEKRGKQQYQRSIHDRSDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            1405..1575
                     /locus_tag="ACHBB7_001694"
     CDS             1405..1575
                     /locus_tag="ACHBB7_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209648.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            1589..2191
                     /locus_tag="ACHBB7_001695"
     CDS             1589..2191
                     /locus_tag="ACHBB7_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9209649.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            2207..2884
                     /locus_tag="ACHBB7_001696"
                     /pseudo
     CDS             2207..2884
                     /locus_tag="ACHBB7_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3195..3692
                     /locus_tag="ACHBB7_001697"
     CDS             3195..3692
                     /locus_tag="ACHBB7_001697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFM9209650.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            3693..4109
                     /locus_tag="ACHBB7_001698"
     CDS             3693..4109
                     /locus_tag="ACHBB7_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFM9209651.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            4129..5673
                     /locus_tag="ACHBB7_001699"
     CDS             4129..5673
                     /locus_tag="ACHBB7_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9209652.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5816..6040
                     /locus_tag="ACHBB7_001700"
     CDS             5816..6040
                     /locus_tag="ACHBB7_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9209653.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            6055..6924
                     /locus_tag="ACHBB7_001701"
     CDS             6055..6924
                     /locus_tag="ACHBB7_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM9209654.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6905..7639
                     /locus_tag="ACHBB7_001702"
     CDS             6905..7639
                     /locus_tag="ACHBB7_001702"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9209655.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7672..8580
                     /locus_tag="ACHBB7_001703"
     CDS             7672..8580
                     /locus_tag="ACHBB7_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM9209656.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            8577..9119
                     /gene="sat4"
                     /locus_tag="ACHBB7_001704"
     CDS             8577..9119
                     /gene="sat4"
                     /locus_tag="ACHBB7_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFM9209657.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            9212..10006
                     /locus_tag="ACHBB7_001705"
                     /note="aph(3')-IIIa"
     CDS             9212..10006
                     /locus_tag="ACHBB7_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM9209658.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            10282..10413
                     /locus_tag="ACHBB7_001706"
                     /pseudo
     CDS             10282..10413
                     /locus_tag="ACHBB7_001706"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            10489..10982
                     /locus_tag="ACHBB7_001707"
                     /pseudo
     CDS             10489..10982
                     /locus_tag="ACHBB7_001707"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            11152..12048
                     /locus_tag="ACHBB7_001708"
     CDS             11152..12048
                     /locus_tag="ACHBB7_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9209659.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            12147..12356
                     /locus_tag="ACHBB7_001709"
     CDS             12147..12356
                     /locus_tag="ACHBB7_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM9209660.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            12373..12645
                     /locus_tag="ACHBB7_001710"
     CDS             12373..12645
                     /locus_tag="ACHBB7_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9209661.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            12647..13510
                     /locus_tag="ACHBB7_001711"
     CDS             12647..13510
                     /locus_tag="ACHBB7_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM9209662.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(13773..14510)
                     /gene="erm(B)"
                     /locus_tag="ACHBB7_001712"
     CDS             complement(13773..14510)
                     /gene="erm(B)"
                     /locus_tag="ACHBB7_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM9209663.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14635..14718)
                     /locus_tag="ACHBB7_001713"
     CDS             complement(14635..14718)
                     /locus_tag="ACHBB7_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM9209664.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         5246 a         2325 c         3038 g         4180 t
ORIGIN      
        1 cgcactatgc cattacattt atatctacgc gaattgtaaa attaagttag aaaaataaaa
       61 aggcatttat ggtacactca aattgtattt ccgacgaaag aaaacaaagg agtgtaacca
      121 taaatgacct acaaacatct taccatagac gaactgacaa tgatagaatc atattatctt
      181 caacataata agccggttga aatcgctaac cgaatgggac gtgctataca aactatttat
      241 aatgtagtca ataagttcaa gcaaggcaag actgctcttg attattggca ccagtataaa
      301 gaaaataaga aaaaatgtgg tagaaaagtc attcaattac ctgctcatga agtagattac
      361 attaaagaga aagtcactct tggttggacg cctgacgtca ttatcgggcg aaaagaaagg
      421 tctgtttcat gcggtatgag aacactttat cgtttatttt ctaaaggaat atttgatatt
      481 gacacactac cgatgagagg taaaagaaaa cccaatggcc atcaggaaaa acggggaaaa
      541 caacagtatc agcgctcaat ccatgatcga tctgataatt atcctgattt caattctgag
      601 tttggtcacc ttgaaggtga tacgatcgtt ggcattcatc ataaaagtgc cgtcattact
      661 ttagttgaaa gattatctaa agtcattatc acgattaaac ccaacggccg taaggcatta
      721 gatattgaaa ctgcccttaa tcaatggttt tctcgcttcc ctaaaaactt ctttaaatct
      781 attacgtttg actgtggaaa agaattttct aactggaaag ccattagtaa ccaacatgat
      841 attgatatat attttgcgga ccctggaaca ccttctcaac gcccattaaa cgagaattct
      901 aacgggattc tgcgtcgtaa tggactgccg aaatcaatgg attttagaaa agtgaatcag
      961 acatttattt ccagtgtcag caatcaacgt aatcatattc caagaaaatc attgaattac
     1021 agaacaccaa tcgaaatatt tttgagctat gtacaagaag cattttattc tagcttaatt
     1081 tgacaaatca tactatgata cgtgtttgtt tttctgtgct gtttagtgaa tgattagcag
     1141 aaatatagag agtaagattt taattaatta ttagggggag aaggagagag tagcccgaaa
     1201 acttttagtt ggcttggact gaacgaagtg agggaaaggc tactaaaacg tcgaggggca
     1261 gtgagagcga agcgaacact tgatctttta agttgctatc tttttaaggt caatagagta
     1321 tacttatttg tcctaatatg gttttagcag tataattgac ttggtgaata ggtcatttaa
     1381 gttgggcata ataggaggag taaaatgaaa aaatttattt atcgagtttt agaaaatgac
     1441 gaagtggtgg ctatttttaa tgagcaacaa tatgcgcaag attttatcgc ttacgaaaag
     1501 acaatttctg ataagcaatt tgaaattgaa aaagtagata tagctgattg gttattgcaa
     1561 ccgagagaat tttagaggtt ggttgaaaat ggctaaaatt ggttatgcac gtgtcagtag
     1621 caaagaacag aacttagata gacaactgaa agcgttagag ggcgtttcta aggtcttttc
     1681 agacaaatta agcggtcaat cggtcgaacg cccacaatta caagctatgc ttaactatat
     1741 tcgtgaaggg gatatagttg ttgttactga attagatcga ttaggacgaa ataataaaga
     1801 attaacagaa ttgatgaatc aaattcaaat taagggggca accctggaag tcttaaattt
     1861 accctcaatg aatggtattg aagatgaaaa tttaagacgg ctgattaata atttagtgat
     1921 tgaattgtat aagtaccaag cggaatctga acgcaaacga attaaagaac gccaagccca
     1981 aggaattgaa attgctaaga aaaaaggaaa attcaaaggg cgacaaccta aattcaaaga
     2041 aaatgatcca cgtttacaac acgctttcga tttatttttg aatggttgtt ccgataaaga
     2101 agttgaagaa caaactggaa ttaatcgccg aacgtttaga aggtatcgat caagatacaa
     2161 cgtgacagta gatcaaagaa aaaaacaatg aaaagaggga tagttaatga gtacggttat
     2221 tttagctgaa aaaccaagcc aggcattagc ctacgcaagt gctttaaaac aaagcaccaa
     2281 aaaagacggt tattttgaga tcaaagaccc actatttaca gatgaaacgt ttatcacctt
     2341 tggttttggg catttagtcg aattagcaga accaggtcat tataacgaaa agtggcagaa
     2401 ctggaaactt gaatctttgc cgatttttcc tgatcgatac gattttgaag ttgcaaaaga
     2461 taagggaaag cagtttaaaa ttgttgctga acttctcaaa aaggcaaata caattattgt
     2521 cgcaacagat agcgacagag aaggtgaaaa tatcgcctgg tcgattatcc ataaagcaaa
     2581 tgccttttct aaagataaaa cgtataaaag attatggatc aatagcttag aaaaagatgt
     2641 gatccgaagc ggttttcaaa atttgcaacc aggaatgaat tactatccct tttatcaaga
     2701 agcgcaaaca cgccaaattg ccgattggtt gatcggcatg aacgcaagcc ctttatatac
     2761 cttgaattta caacaaaaag gcgtacaagg tacattttca ttaggacgtg tacaaacacc
     2821 gactttatat ctcatttatc aacgccagga agcaatagaa aactttaaaa aagaaccttt
     2881 tttcctgaat aattcatagt tttactaata gtctttccta acattgattt aatagtcttt
     2941 aaagtattct gcttcatcac ccattaggtg atgaaaaaag aacccctttc tgatttagcg
     3001 gacaaatact ttgacctaat ctcagaagat ataaaagaaa aaataataaa atatccgatt
     3061 tatactttgt ataaagaact aaagtagtta gcaaatcatt tacagcaaat cactatgttc
     3121 cacataccat tttacatact taccttatcc acgacacatg ttgagatagt atcgaagata
     3181 aagaaagttg ctccatgttg tttttgcgag atggggaaat caaagccaca ttgacaacca
     3241 tgatgaataa gcttgctttc agtcataagc taatcctaga accacttttc aagtcagtta
     3301 gccaaatcga tgaagaaagc gaccgtgaaa gaatggacgc tattgataag ctaatggagc
     3361 aactattgga agaacgcaat acccttattg cactgatgtc caaaggtttc ctagaacctg
     3421 ctctttttaa tcaggaacga aatgttttag atagtgagat taaaaatctt actaccgaaa
     3481 aaacaaacct ggtaacgaac tctgcgagtg gggttttgcg agcaaacgag ataaaggacc
     3541 tcattaatta tgtgtcagca gataatttta atggtgacta cacggaagaa ctatttgaag
     3601 aatttgtagt gaacatcatt gtaaattcca gggatgagct gacattcaat ttgaaatgcg
     3661 gtctttccct gaaagaaaag gtggtgagat aaatggcata tattccatat ggatacaaaa
     3721 ttcaagatgg agtggttact gtcgatgaaa aggcagcagg tcaagtaaag gtattctttg
     3781 agaaatacat atcaggacta tcccttacag tggctggcga acaggcaggt attgaaaaga
     3841 cacattctgt gatggggcgc attttgaaaa acgtcaacta ccttggaaat gatacgtatc
     3901 cagcaattat tgataaagag atatttgata aagctgaaga agttagagat aaacgtgcaa
     3961 aggatttagg acgagtggta gagcttgccg ctttcacctc tccccctccc aaagaacgat
     4021 tcaaaatgaa aaaggcagat aataagatgc cagtggatcc ttttgaacga gcagaatact
     4081 tatacagtct gatagaaagc gaggaataaa gtgacagaga aaaatataat ggttattcct
     4141 gctcgtaaaa gagtaggaag tacagccgca aaagaaaaga taaagaaact tcgtgttgct
     4201 gcctattgcc gtgtttctac agaaacagaa gaacaaaatt ctagctatga ggttcaggtc
     4261 tcacactata cagagtttat aaagaaaaat aatgaatggg agtttgctgg catctttgca
     4321 gatgatggta tctctggtac gaacactaaa aagcgtgacg aatttaatcg catgattgca
     4381 gagtgtatgg acggtaacat cgacatggtt attacaaaat ccatcagccg atttgcgcgt
     4441 aacaccctag actgccttca atacattaga cagctcaagg ataagaacat atcagtttat
     4501 tttgagaaag agaacatcaa caccacggat gccaaaggtg aggttttgct gactattatg
     4561 gcatctttgg cacagcagga aagccagagc ctttcacaaa acgttaagct tgggctacag
     4621 taccgatacc aacaaggaaa ggtgcaggtc aaccataagc gatttatggg ctacacgaaa
     4681 gatgaagatg gaaatttaac tattgtcccc gaagaagctg agattatcaa acgcatctac
     4741 cgagaatacc ttgagggtca gagtctagtg ggcattggtc gagcccttga aaaggatggt
     4801 attttaacag cagcgggaaa accaagatgg cgaccagaat cagttaagaa gatacttcaa
     4861 aacgaaaaat acatcggaga tgcccttctg caaaagactg tcacagtaga ttttctgacc
     4921 aagaaacgag tgaaaaatga agggcatgtt ccccaatatt atgttgaaaa tagccatgaa
     4981 gcgattattc ctaaagagtt atttttgcag gcgcaagaag aaattcatcg gagaagcaat
     5041 atctacacag gagaaggcaa gaacaaaaga atttacagta gcaagtacgc tttaagtgcc
     5101 atcaccttct gtggagattg tggcgatatt tatagacggg tctattggaa tatccacgga
     5161 agaaaagaat ttgtctggcg atgcgtgact agaatcgagc aaggtcctga agtttgtaag
     5221 aaccgaaccg taaaagaaga tgaactttat ggtgcagtaa tgactgcgat taataagcta
     5281 cttgcaggtg ggaacaatat gataaagaca ctggaagaga atattcatgc tgtgattggc
     5341 gaaaccacag aataccaaat ttcagagatt aacaactcac tagaggaaaa acaaaaagaa
     5401 ctaatcaagc tggcgaacaa gggtcaagac tatgaccact tagcagatga gattgatgag
     5461 ctgagagaca agcgacagat ccttttagta gaagatgcct ccctcagtgg cgagaacgag
     5521 cgaatcaatg agctgattga atttatccgc aagaacaaat tccgcacctt agaatatgat
     5581 gataagcttg taaggaagat aatccagagc gttacagtct atgaaggcca cttcgtcata
     5641 gccttcaaat ctggcatcga aatggaaata tgaaaaccag aagtaaatag cccatgactc
     5701 tacagtagag ttgtgggttt tcttttgctt gctgattata ataatatttt aataactatt
     5761 gtgtttatca accaaatggt ttataatatt cttagcgtaa acttggaggt gctgtatgac
     5821 tacggcagaa atgattaaag aactgtgtga gcaaatgaat ataagtgttt ccgaacttgc
     5881 tagacgtatt ggccagactc cacagaattt caataaaaaa ttacaacgag aaacagtaac
     5941 cttggatgag ttgaaggcca ttgctgatgt gctaggtgtc aagtttgtgc aggcatttat
     6001 tttaccagat ggagaagaaa taaaaatatc taacgaataa aggaggatgt ttatatggta
     6061 ggtaatgtta ttcaaatagt aacagagaaa ttatcatctt tgccttttat agaaggcatc
     6121 gtattagggg gctcacgtgc aagaggcacc catacagaaa attctgatat agatatcgga
     6181 atctattaca attcagactc atttgacctg acagcgatta atcaaattgc tacagagttg
     6241 gatgatgaga atagaaacaa ccttgttgta cctcccggag cgtggggtga ttgggttaat
     6301 ggcggtggat ggttagttat aaacgggtgt catgttgact tgatattacg tgatataaaa
     6361 cgggtggaac aaataatcaa agatacggag caaggaattg ttactgccaa ttatcagacc
     6421 gggcatcccc atggttatat aagtgctatg tatcgtggag aattagcgat tagtaaaata
     6481 ctatatgcta agaacgaaag cttatgcgaa ttaaaaaagc aggcagaaat ttaccctact
     6541 gctctaaaga aaagcttgat gaactttttt atatttgaag ctgaattctc tttaatgttt
     6601 gtaaaagcaa atgcgggggc agaggataaa tattatattg caggccatgt ttttcgtatt
     6661 atttcatgct taaatcaagt actatttgca tgtaataatg cttattgcat taacgaaaag
     6721 aaagctataa aactgcttga aacttttgaa tataaacctg aaaaatatgc cgaaagagtt
     6781 aatcatattt ttgaagtact cggtctttct ctttttgaat gctacgacat gaccgagaaa
     6841 ctttataaag aagtgaatga aattgtatcg gagataaata actttttaaa cgaggagagt
     6901 tcagatgaaa gaaaacaaat atgatgataa tatatttttt caaaaataca gtcaaatgag
     6961 tcgctcgcag aaaggactgg ctggtgcggg agaatgggag actttgaaaa agatgctacc
     7021 tgattttaag ggtaagcgtg tgcttgattt aggatgcggc tatggatggc actgtatata
     7081 tgcgatggaa aacggtgctt cctctgtagt aggtgttgat atttctcata aaatgctcga
     7141 agtagcaaaa ggaaaaaccc attttccaca gattgaatat gaatgctgtg ccatagaaga
     7201 tgtggatttc ccagaggaga gctttgatgt aatactaagt tcgcttgcgt ttcattatgt
     7261 agcagactat gagaatttaa taaaaaagat atataggatg ctgaaggctg gtggcaattt
     7321 agtttttaca gttgaacatc ctgtttttac tgctcatgga acacaagact ggtattataa
     7381 cgaaaaagga gaaatactgc atttcccggt ggacaattat tattatgagg gcaaacggac
     7441 agctatgttt ttggaagaaa aggttacaaa atatcataga acactgacca catatctaaa
     7501 tacactgctt tcaaatagtt ttataataaa tcagattgtg gagccacagc cgccagagaa
     7561 catgatggat attccgggga tggcggatga aatgcgacgc ccaatgatgc tgattgtatc
     7621 ggcaaaaaag aagatgtaat aatatagaaa aaataaacga ggagtatgta aatgagatca
     7681 gaaaaagaaa tgatggattt agtactttct ttagcagaac aggatgaacg tattcgaatt
     7741 gtgacccttg aggggtcacg cgcaaatatt aatataccta aagatgaatt tcaggattat
     7801 gatattacat attttgtaag tgatatagaa ccgtttatat ctaatgatga ctggcttaat
     7861 caatttggga atataataat gatgcaaaag ccggaggata tggaattatt cccacctgaa
     7921 gaaaagggat tttcctatct tatgctattt gatgattaca ataaaattga tcttacctta
     7981 ttgcccttgg aagagttaga taattaccta aagggcgata aattaataaa ggttctaatt
     8041 gataaagatt gtagaattaa aagggacata gttccgactg atatagatta tcatgtaaga
     8101 aagccaagcg caagggagta tgatgattgc tgcaatgaat tttggaatgt aacaccttat
     8161 gttattaaag gattgtgccg taaggaaatt ttatttgcta ttgatcattt taatcagatt
     8221 gttcgccatg agctgctgag aatgatatca tggaaggtcg gcatcgaaac aggctttaaa
     8281 ttaagtgtag gcaagaacta taagtttatt gaaaggtata tatccgagga tttgtgggag
     8341 aaacttttgt ccacctaccg gatggattcc tatgaaaaca tatgggaagc attatttcta
     8401 tgccatcaat tgttcagggc ggtatccggt gaggtggcgg aaaggcttca ttatgcctat
     8461 ccggagtatg ataggaatat aacaaaatat accagggaca tgtataaaaa atacactggt
     8521 aaaaccggct gcctggatag cacatatgcc gctgatatag aagagaggcg ggaacagtga
     8581 ttacagaaat gaaagcaggg cacctgaaag atatcgataa acccagcgaa ccatttgagg
     8641 tgataggtaa gattataccg aggtatgaaa acgagaattg gacctttaca gaattactct
     8701 atgaagcgcc atatttaaaa agctaccaag acgaagagga tgaagaggat gaggaggcag
     8761 attgccttga atatattgac aatactgata agataatata tctttactac caagacgata
     8821 aatgcgtcgg aaaagttaaa ctgcgaaaaa attggaaccg gtacgcttat atagaagata
     8881 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
     8941 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
     9001 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
     9061 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     9121 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     9181 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
     9241 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
     9301 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     9361 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     9421 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
     9481 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     9541 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     9601 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
     9661 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
     9721 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     9781 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     9841 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     9901 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     9961 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
    10021 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
    10081 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat agagttctat
    10141 attagagatg taaagagttt tggtgaagag gtttgtgagt atggctttta taaccagggt
    10201 gcaatgagaa gcactaacag cagctagctt aagaacgcat aggatatact ttttatggaa
    10261 gtcacagagg aggaataaaa tttgaacagg attaacagag taacctctat tcttattcag
    10321 ctgcaatcaa aaaaaataat tcctgccaaa gaaattgcac agcgatttaa tataagctta
    10381 aggacagttt acagagatat ccggacactt gaagaagccg gaataccaat tggatctgag
    10441 gccggaaaag gatattttct tgtagagggc ttcctacttc cgccggtaat gtttacagcg
    10501 gcggaagtgg gtgcattgat tacagcaggg aaatttttaa attgccatgg agatgaatca
    10561 tttataaagg attttgattc agctatgtat aaaatcaaat ctattttgaa gcatggtgaa
    10621 aaaaactatg cacaggagtt ggagaacagt attaacgtgt acagcacatc tggacagaaa
    10681 aatacattgg cggataacgt tattgcagca attcagactg caatctgcaa taaaagggta
    10741 atatcaattc aatatcctgc atcaggaggg caagaaccgg aaagcagaat gatagagcct
    10801 atatcactgg ggttttatga acagaactgg tacttaatcg gttttgcagg ctaagaaatg
    10861 aatataggaa ttttcgtgtg gatcggatta aaagcatttg catagaagaa gagctctgtc
    10921 aaagccatga tggctctttg gaacaaatat taaaacaaat gctttcatat aaaaaactat
    10981 atgccctaca atgccgttgt aaaaattggg gaaaagggat atattgattt tatatctttc
    11041 tcaaaataaa tataaaagcc ctttaaagag ggcttttata tattaatcac aaatcactta
    11101 tcacaaatca caagtgatta atcacaaatc acttgtgatt tgtgattctt aatgatacaa
    11161 tattactata caaaaaaaga atggggcgta gttatggaga aggaaaaact aaaaatactt
    11221 gaagaattaa gacgtatttt aaacaataaa aatgaagcaa ttattatctt gaataattac
    11281 tttaaaggtg gtgttggaaa gtccaaatta tcgactatgt ttgcttactt gacagacaaa
    11341 ttgaatttaa aagttttaat gatcgataag gacttacagg caacattgac aaaagactta
    11401 gcaaaaactt ttgaggtaga attgccacgt gtcaattttt atgaaggctt gaaaaatgga
    11461 aacttggctt cttctattat tcatttgact gataatttag acttgatccc tggcacgttt
    11521 gatttgatgt tactgccaaa attaactcgc tcatggacgt ttgaaaatga aagtagattg
    11581 cttgctactc ttttagcacc tttaaaaagt gactatgatc ttattattat tgatactgta
    11641 ccaacgccaa gcgtttatac aaataatgca atcgtggcga gtgattacgt tatgatccct
    11701 ttacaagcag aagaagaaag tacaaacaac attcaaaact atatttccta tttgattgat
    11761 ttgcaagaac agtttaaccc tggactagat atgattggtt ttgttcctta tttagttgat
    11821 acggacagcg caacgataaa atcaaacctg gaagaactgt acaaagaaca taaagaggat
    11881 aatttggttt tccgaaatat tatcaagcga agtaataaag taagtacttg gtctaaaaat
    11941 ggcattacag aacacaaagg ctatgacaaa aaagttttgt ctatgtatga gaacgtattt
    12001 tttgaaatga ttgagcgaat cattcaatta gaaaatgaaa aagaatagaa tcacaaatca
    12061 caagtgatta atcacaaatc acttgtgatt tgtgataggt gatgataaaa taaatagtaa
    12121 gaagaaatag aaagaagtga gcgatcgtgg gaaatttagg cgcacagaaa gcaaaacgaa
    12181 atgatacgcc aatcagcgca aaaaaagata taatggggga taagacggtt cgtgttcgtg
    12241 ccgacttgca ccatatcata aaaatcgaaa cagcaaagaa tggcggaaac gtaaaagaag
    12301 ttatggatca agccttagaa gaatatatac ggaaatattt acctgacaaa ctttaaaagg
    12361 agtgaaaatg aaatggcagt tacgtatgaa aaaacatttg aaattgaaat cattaatgag
    12421 ttatcggcaa gtgtttataa tcgggtatta aattatgttt tgaatcatga actagatact
    12481 aaaaatactc gtttactaga agtgaatctt ttaaatcaat tagaagtggc acaagaagtt
    12541 gatttatttc aacaaccatt tgaagaatta caagctattc atgagtattg gcggtcaatg
    12601 aatcaatatt caaaacaaat tttgaataaa gagaaagtgg cttaacatgg cgaatattac
    12661 tgacttcacc gaaaagcaat ttgaagatcg tttagaaaag aatgttgaac gactaactaa
    12721 aaatagacta gcggttgaat cgccaaccgc ttttttactt ggtgggcaac cagggtcagg
    12781 gaaaaccagt ttgcgatcag caatttttga agaaacacaa gggaatgtta ttgtcattga
    12841 taatgatacc tttaaacaac agcacccaaa ttttgacgaa ttagtgaaac tctatgaaaa
    12901 agacgtagta aaacacgtta ccccttattc gaatcgcatg acagaagcac ttattagccg
    12961 tttaagcgat caaggctata atttagtgat tgaaggaaca ggacgaacaa cagacgttcc
    13021 cattcaaaca gccacaatgc ttcaatctaa aggttatgaa acaaaaatat acgtcatggc
    13081 agtaccgaaa attgaatcat acttaggaac gattgaacga tatgaaacca tgtatgcaga
    13141 tgatccaatg acagctaggg caacaccaaa acaagcgcat gatattgttg tcaaaaactt
    13201 accgaccaat ttagaaaccc ttcataaaac gggcttattt agcgatataa ggctttacaa
    13261 tagagaagga gtgaaactct attcaagctt agaaacgcct tccattagtc cgaaagagac
    13321 cttagaaaga gaattaaatc gtaaagtatc agggaaagaa attcaaccga ctttggagag
    13381 aatagagcaa aaaatggttc aaaatcaaca ccaagaaacc cctgaattta aagcaattca
    13441 acaaaaaatg gaaagcttgc agctacctac accaccaata cccaaaacac ctaaacttcc
    13501 aggtatttaa accttaaaaa aggaaaagag tagttaccaa aaaacggtaa ctactctttt
    13561 tttataaaaa catttcctca tgttggggtc ccgagcgcct acgaggaatt tgtatcgata
    13621 agaaatagat ttaaaaattt cgctgttttt ttctaatttc attttgaggt gaattaattg
    13681 gtagttatta gaggtgccct atacctaata atttatctac attcccttta gtaacgtgta
    13741 actttccaaa tttacaaaag cgactcatag aattatttcc tcccgttaaa taatagataa
    13801 ctattaaaaa tagacaatac ttgctcataa gtaacggtac ttaaattgtt tactttggcg
    13861 tgtttcattg cttgatgaaa ctgattttta gtaaacagtt gacgatattc tcgattgacc
    13921 cattttgaaa caaagtacgt atatagcttc caatatttat ctggaacatc tgtggtatgg
    13981 cgggtaagtt ttattaagac actgtttact tttggtttag gatgaaagca ttccgctggc
    14041 agcttaagca attgctgaat cgagacttga gtgtgcaaga gcaaccctag tgttcggtga
    14101 atatccaagg tacgcttgta gaatccttct tcaacaatca gatagatgtc agacgcatgg
    14161 ctttcaaaaa ccactttttt aataatttgt gtgcttaaat ggtaaggaat attcccaaca
    14221 attttatacc tctgtttgtt agggaattga aactgtagaa tatcttggtg aattaaagtg
    14281 acacgagtgt tcagttttaa tttttctgac gataagttga atagatgact gtctaattca
    14341 atagacgtta cctgtttact tattttagcc agtttcgtcg ttaaatgccc tttacctgtt
    14401 ccaatttcgt aaacggtatc ggtttctttt aaattcaatt gttttattat ttggttgagt
    14461 actttttcac tcgttaaaaa gttttgagaa tattttatat ttttgttcat gtaatcactc
    14521 ctgaagtgat tacatctata aataaataca gaagttaaac gatttgtttg taattttagt
    14581 tatctgttta aaaagtcata agattagtca ctggtaggaa ttaatctaac gtatttattt
    14641 atctgcgtaa tcactgtttt tagtctgttt caaaacggta gatgttttat ctacattacg
    14701 catttggaat accaacatga cgaatccctc cttcttaatt acaaattttt agcatctaat
    14761 ttaacttcaa ttcctattat acaaaattt
//
LOCUS       DAYYKN010000066        14711 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000066 DAYYKN010000000
VERSION     DAYYKN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.066"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..622)
                     /locus_tag="ACHBB7_001714"
     CDS             complement(<1..622)
                     /locus_tag="ACHBB7_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209665.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPE"
     gene            complement(801..1520)
                     /locus_tag="ACHBB7_001715"
     CDS             complement(801..1520)
                     /locus_tag="ACHBB7_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9209666.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1651..2802)
                     /locus_tag="ACHBB7_001716"
     CDS             complement(1651..2802)
                     /locus_tag="ACHBB7_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM9209667.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3019..3759)
                     /locus_tag="ACHBB7_001717"
     CDS             complement(3019..3759)
                     /locus_tag="ACHBB7_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9209668.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3763..4086)
                     /gene="rsfS"
                     /locus_tag="ACHBB7_001718"
     CDS             complement(3763..4086)
                     /gene="rsfS"
                     /locus_tag="ACHBB7_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM9209669.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4143..4736)
                     /gene="yqeK"
                     /locus_tag="ACHBB7_001719"
     CDS             complement(4143..4736)
                     /gene="yqeK"
                     /locus_tag="ACHBB7_001719"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM9209670.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4720..5370)
                     /locus_tag="ACHBB7_001720"
     CDS             complement(4720..5370)
                     /locus_tag="ACHBB7_001720"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM9209671.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5380..5691)
                     /gene="yhbY"
                     /locus_tag="ACHBB7_001721"
     CDS             complement(5380..5691)
                     /gene="yhbY"
                     /locus_tag="ACHBB7_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM9209672.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5707..6816)
                     /gene="yqeH"
                     /locus_tag="ACHBB7_001722"
     CDS             complement(5707..6816)
                     /gene="yqeH"
                     /locus_tag="ACHBB7_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM9209673.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6813..7340)
                     /locus_tag="ACHBB7_001723"
     CDS             complement(6813..7340)
                     /locus_tag="ACHBB7_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM9209674.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7520..8305)
                     /locus_tag="ACHBB7_001724"
     CDS             complement(7520..8305)
                     /locus_tag="ACHBB7_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM9209675.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8298..9167)
                     /gene="accD"
                     /locus_tag="ACHBB7_001725"
     CDS             complement(8298..9167)
                     /gene="accD"
                     /locus_tag="ACHBB7_001725"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM9209676.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9168..10547)
                     /locus_tag="ACHBB7_001726"
     CDS             complement(9168..10547)
                     /locus_tag="ACHBB7_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM9209677.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10555..10974)
                     /gene="fabZ"
                     /locus_tag="ACHBB7_001727"
     CDS             complement(10555..10974)
                     /gene="fabZ"
                     /locus_tag="ACHBB7_001727"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM9209678.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10999..11475)
                     /gene="accB"
                     /locus_tag="ACHBB7_001728"
     CDS             complement(10999..11475)
                     /gene="accB"
                     /locus_tag="ACHBB7_001728"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM9209679.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11478..12713)
                     /gene="fabF"
                     /locus_tag="ACHBB7_001729"
     CDS             complement(11478..12713)
                     /gene="fabF"
                     /locus_tag="ACHBB7_001729"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM9209680.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12732..13469)
                     /gene="fabG"
                     /locus_tag="ACHBB7_001730"
     CDS             complement(12732..13469)
                     /gene="fabG"
                     /locus_tag="ACHBB7_001730"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM9209681.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13481..14398)
                     /gene="fabD"
                     /locus_tag="ACHBB7_001731"
     CDS             complement(13481..14398)
                     /gene="fabD"
                     /locus_tag="ACHBB7_001731"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM9209682.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14505..>14711)
                     /locus_tag="ACHBB7_001732"
     CDS             complement(14505..>14711)
                     /locus_tag="ACHBB7_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9209683.1"
                     /translation="QEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINEIEDEFD
                     VKIETEDGIKTVQDLVTYVEKQTA"
BASE COUNT         3868 a         3370 c         2529 g         4944 t
ORIGIN      
        1 tttcaggata ttttaatttg acaagcgaaa taatcatttt cagttcttcc atgttttctg
       61 gatattctgg gtcaaatacc ttgctatgac ggccaatccc gccattgtcg atcaatcggt
      121 aaattcgatt ccattcaatg aattggtcaa ttgattggaa attgctgatt ggatagccgc
      181 cacaatccaa aatctctgat acacgaaatg ctaggtatgc actatcgatc actttatttt
      241 ttttcgtgtc tatcttctca tttctttcga taagaatttt ttcaggacga tgtttcaagc
      301 tccactctaa tagtagaaag aaagcctcca tccgatactc ttcttcaaat aaccgaaagg
      361 ctgaatcaga acgacgaaaa agcagcggaa tcagataatt ttgtctataa atcagcattg
      421 gtgcgcggtc caccatacac tctaaataac ggttcagata ccctgtaccg acatagtttc
      481 ctaatcgata gccttcaacg ccgaatcttg cgtatttttg cgctaatttt gaacaatcag
      541 ttacgttcac agctaactct tctactttgc ggtaaccgct taagatacgt tcaaaaggtt
      601 ctaaagtgat ttttgcttcc atattcttcc ccgtccatct tccacatact tcatatcatt
      661 tcacatgata cttatgctct catcatacca caggttgaat tagaaaagaa aataaatcac
      721 acaaaaaagc aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac
      781 ttttcgttaa aactggttgc ttagtcttct aatgccacat tatggaacac atgctgtaca
      841 tcttctagat cttccagtgc atcaatcatt ttttcaaatg tttcttgatc ttctggagat
      901 agtgttacct cgttttgagg gatcatttgg atctcagcga ttgaaaaatc agtgattcct
      961 ttttctttta atgcttcttg tatactatgg aaatccgcta cttcaccgta aacgatgatt
     1021 tgtccttctt cttccattac atcgcgaaca tcgatgtctt tttccattag gtattcaagg
     1081 atctcgtcag cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa catgtaagaa
     1141 accgctccgg aaacacccat gttcccgcca tttttcccaa atgctgcccg tacgtctgct
     1201 gctgttcgat tcacattgtt cgtcaatgta tcaacaatca ccattgagcc gcttggtcca
     1261 aatccttcat agcgcaattc agagtatgtt tcatctcctg caccttttgc tttttcgatt
     1321 gctcggtcaa taatatgttt tggtacatta taagttttag cgcgatcaat gacgaaacgt
     1381 agtttttggt ttgcatgcgg atcaggatca cctgattttg ctgccgcgta gatttcaatc
     1441 ccaaattttg cataaatccg gctattatta gcatctttag cagttttctt tgctacgata
     1501 tttgcccatt tacgtcccat attttcactt cgctttctga tttgatagtt tcataacgca
     1561 aaaacgtcct gttccattat acttgtgaat cacgattctg tgaagctttt tttctttctt
     1621 ccttcattct tctcattttt agtcaattat ctatactcgg attgggattt gcccgaaatt
     1681 ctgttccgga atattgcctc ccaattgata gatttgatca cttctaacta acagttccgc
     1741 ttcttgtgcc tctttttttc cgattcggga ataaatagaa atctcttttt tttcttttaa
     1801 gaatcttctt ccagcatcgg taaatccaag tagtcgaaga taatttttat tttgttggac
     1861 aggaatctct tgctttttga tattcagcaa gacataacaa gataatcgtt ggatccgtgt
     1921 ccatgtatat ctttttgttt ttacctttga cacaaaatcc tcaaaactat cagcagttgc
     1981 agctgctgtt ttcatgcgcg attctattcc ttcggtcatc tgatagatat ctgtcagttc
     2041 cagcaaagta gaagataaca accgataacg caaatacggc caataagatt cccaagtctg
     2101 cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc acaagtccag ctattctttt
     2161 tccttcttct aacgcatggc gaatggctgt tgcactggct atcctctgat ctgtgatgat
     2221 tttttcatga tagcctgcac ttttacgtcg gatcggatgt aattccatag ggtggacata
     2281 cttggcattt tcctttgcat aacttaatcc caagatatga tttggcgaag aaaaatcaag
     2341 cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt
     2401 ttgatcagac agttcttgaa atgcctcttc tatcctatct tgattctctt ggacaaagcg
     2461 tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca
     2521 gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa tagtcagctg actgaaccgc
     2581 ccattcgacg ggaagctcca ctactaaatc cacaccattt tgtaaagctt cattcgcgcg
     2641 atgccattta tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc tcattacagc
     2701 aatgacgacc tctgctcctg ttatttttcg tgccatttcc acatgatagc gatgtccatt
     2761 gtgaaaagga ttgtactcta cgataatccc gcagcttttc atgcgcatcc tccttcatag
     2821 ttatcgataa aaaagaaata gagacaagta aaaaaaggca aaaatcggaa aaaatacccg
     2881 aactatataa actattctcg caaacacaat acttctgcga cgaaaagctg cactgcaaga
     2941 gcaatgccta tatcgttcgg atatataatt tatgttgaag atttattctt ttcttgttaa
     3001 ctcgagcagg caaaaccgct agtctttgta tgctacaaag aaccagcgct ttgatttctc
     3061 tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc
     3121 cagcattctt aaataacttt ccaatggata ggtacgctct tgatgtagtt catcttcacg
     3181 aataaatcga ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct
     3241 gtgtttctcg cttcctggat aactatccca caaaaaagca aaattatccc cttgataatg
     3301 atagctatat tctggaaaaa cctcatctac ttgataggtg gagtgtacgt caaataaaaa
     3361 tcggccattt tcttctaacg cttgatagac ttcatcaaac acttgctgta cctcttgtct
     3421 atttttcata tagcacagcg aatcagaaaa acaggtaatt gcctgatatt gtccgatttc
     3481 tgataaatcg agcatattgc cttccacaaa ttggatcgat gcgtcttctt catatgctcg
     3541 ctggctggca atcatcaaca tctcttctga aaggtctaac cctgtcactt tatatccatc
     3601 ttttgcaaag gcaaccgcta atgctccagt accgcatgcc agttccaaaa tattttttgt
     3661 gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg tttcgtccat
     3721 cacttcatcg taaatgaaag caaatgtttc ataagccata tgttaatcca accattcact
     3781 aagatcgact agcggagcat ctgaccatag tttttccaga ttatagaaat ttcgttctgg
     3841 cgcatggaaa acatgtacga tcacatctcc aagatcgatc aatacccatt ttccgccttc
     3901 ttttccttca atacgtttta cttcatattt gttttcttct tctttatcaa tgatttcttc
     3961 tgtaattgcg ttgatttgac gttcgctatt tgctgaacag atcatgaaat aatctgccaa
     4021 caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg aatctgctgc
     4081 ttcaatcgca atttttaatt gttctttact atctatgatg atttcctcct aatatgagca
     4141 gcttattttt ttgctaccca gtaattatat gtttcgattg tttttggata aatctgttgt
     4201 tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa atgctacagc ttcgtctaga
     4261 tcagcccatg caatggctcg tgcatcttgt accccaggaa aatctcgacc tggttcaata
     4321 tagtctgcta cataaatgat tttgtcaagg agactcatct tagcagcccc agtcgtatgc
     4381 acccggattg catgaagaat ctctgcatcg gtgattccta attcccgctc tacaaacgaa
     4441 gcgccaacaa gaccatgcca gattgcatta ttataattca ataactccgg atcaaaaccg
     4501 tctcttacga ttaccatttt gaactcctca tctggtcgtt cttttgcata atcatgtgtc
     4561 aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt ttttggcaag agccacggct
     4621 gtttcttcta ctccaagcac atgcttgaag cgttgttcgc tcattctcat ctgtattttt
     4681 tgcatcaggt tttctctaga ataagcagta tattcattac tatagtccat attcatacaa
     4741 cccttcattt tgaatataat caattacttt atctggaaca aggtagcgga tagaacagcc
     4801 ttctttgatc ttttgacgta ttttcgtgga actgatgtcg atctcaggta catctaccca
     4861 aattacagga tatggagtat ccgttgtgta tcctggtcga cgtataccca caaagttgac
     4921 catagaagtc agctcatcga ttttatacca ttttggcaaa tattctacca tatccccacc
     4981 gataataaag taataatctg tatccggatt gttttgcgtc aattccttca tagtatcata
     5041 ggtgtaactt ttcccaccac gagctaattc aatcgtttca atctgcaaaa aaggattgtc
     5101 ttccaccgct aattccaaca tgttgagtcg atgttttgca tcaatcgtct gtttctcatc
     5161 aacatgaggt ggctgataag aaggcatcaa aaatacgcgg tcaagaccta aattccggcc
     5221 agcttgttca gccatcacta aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat
     5281 ccctacttgc tttttcttct ctaagaattg aggcatttct tcaggaaata attgtgaacg
     5341 aacaaatgtc ttagcttgcg tattcatcat ggtcactcct tatattgctt ttacttcttt
     5401 tgatagcctt tgatactttt cttttgtgga aggtttgtat aatacaataa cacgtccaat
     5461 gatttggacg atttcgcaat ctatttcttt ttctaatact tgagctgctt cttctgcaat
     5521 ctcatctgta ttttgaagta acgatacttt gatcagttct cttttttcta aagcttcttc
     5581 aatttgaacg atcattgctg aattgatccc accttttccg atttgaaaaa tcggctgaag
     5641 atgatgcgct ttgctacgta aaaaacgctt ttgttttcct cttaaactca taattttcct
     5701 tcttccttaa atcaatgctt tgcgtttcaa tacatctaca ccctttggcg cccagcccgc
     5761 aattactact ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt
     5821 ttctttcaca gaaaactcaa aacgaacaag ctccggaaat gcctcgactt catcaggacg
     5881 aggaggctgt aataatccac ctacatgttt ttgataaaat tcgtctgctt tttccagttt
     5941 cgttcgatga atatttacat catttgacac ataagcaata aatgaagcac gattgccgct
     6001 gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata
     6061 aactttaggt ttgatttctt ttaacggtgc aatgatccgc aaatcttttt tacctaagta
     6121 gtgcgccatt tgatggcgat ggataattcc cggtgtatca atcaaaaaat gcccgtcatc
     6181 taaaggaatc tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc
     6241 ttgtactccc gctgtatttt tgatgatttg attgataagc gtcgattttc cgacatttgt
     6301 tactccgacg atatagacat cccgtccatc gcggtattct tcgatttttt ctaatagttc
     6361 cgtcatctca tgagatttct tagcactagt taataataca tctactggac gaattcctgc
     6421 ttcgtaagca cgttccttca tccattgtgt taatttagtt tttttcaatg atttaggtaa
     6481 tatatccact ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc
     6541 tgggattagt gaaccattga agtcaaaaat atccaccaca ttgacgatca atgcatcttt
     6601 ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga catcttgggt
     6661 ctcattataa tggcgtaatc tgaaacaacg ctgacaatag acttccccgg aagctaaacc
     6721 tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg tttgtataac
     6781 ggcaccacag ccgatacatt gtaattcttc tgtcattcga ttccacctcg ccagcccata
     6841 tcagggtgtt ttgctaatag ataggccata attctttttt cccaaaaacg attaaattgt
     6901 gtcttccaac tatctgttgc gataatgggt tttacaagga tgctacgtat ccctgcacga
     6961 ttggctccac gtatatcagt cataatctga tcgcctacca ttactatctc atcatctgct
     7021 aaatccaatt gtttctttgc tatgttgatc cctcttgcta aaggtttcat tgctcgtgca
     7081 acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa
     7141 acaacaacaa cagggattcc tgcatttttc atttctaaaa tccaagtaag caactcttct
     7201 gttccatctg gattattcca tgcaatcaac gtattgtcta aatcggtcaa tactgctttg
     7261 atgcctagat tctttaattg ggcaggtgta atctgataga tggcttctac catccatgtt
     7321 ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa aaatgaagga gcacataaag
     7381 tactcctcaa ccccgtatta tactgcatat cagcaaaaaa aactacctct atactcgttc
     7441 gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa
     7501 atttagaaac aaaaaacaac taatattggc ggaacctctt gtatcgtttt tccagtaatt
     7561 tatgctgatc taatcgaaca agttccgtca atctttcaat caacgctttt ttcataatgt
     7621 gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt ttcaggaata actcgatcaa
     7681 cgattcctaa ctcgcgtagt tctggcgcag taattttcat caactcagct gcttctgcgg
     7741 cgcgactacc gtctttccac aaaatcgacg caaatccttc tggagaaagc acagcataaa
     7801 tcgtttcttc cagcatccag acttcatccg ctaaacctag agcaagggca ccgccacttc
     7861 caccttcacc aatgatgatg gcgatgatcg gtacctttaa atcagacatt tcgagcagat
     7921 ttctggcaat agcctctcct tcgccgcgct cttctgctcc tacaccgcaa tatgcacctg
     7981 ctgtattgat caaggtaatc accggtctat tgaacttttc cgcttgtttc atcagtcgta
     8041 aagcttttcg atagccttca ggatggggag aaccaaaatt tctgcggata ttttcctgca
     8101 gatcccgtcc tttttgaata ccgatcactg ttaccggttt gccatctaat cgagcgattc
     8161 caccaacaat cgcttcgtca tctgcaaata aacgatcacc atggcattcc tcgaaagaat
     8221 cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt gctaactgaa
     8281 cgacatcatg tgctgtctta tccacgatct atccatcctt tctgtgtatg caattcaaga
     8341 agcgtatgga ttttctgttt caattccatg cggggaacaa tctgatcgac aaaaccatga
     8401 gataataaga attcagcctt ttggaaatct tctggaagtt cctgcttgat cgtctgttcg
     8461 atgacgcgtc ttccagcaaa accgatcaag ctttggggtt ctgctagaat aatatcgcct
     8521 tccattgcaa aactggcagt tactcctccg gtcgtcgggt ccgttaaaac agttagataa
     8581 aaaagacctt cattgctatg ccgtttgaca gcagcagaga tttttgccat ctgcattaga
     8641 gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac gactggaaga
     8701 ctttcctttg ttgctcgttc gaataatctg gtgatctttt ctccgacaat cgttcccata
     8761 cttcccatga taaaattagg atccatcact ccaataatga agggaatccc ctgaatggtt
     8821 gctttaccag tgaggactgc ttcatgaagt cctgttttat cttgcatttt tttaattttt
     8881 tccgtatatt cgggaaatgc tagcggatct ttggttacaa gatccgtatc ccattcttca
     8941 aaactttttt catcaataat caaagctaga cgctgccatg caccgattcg aaaagcgtat
     9001 ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg tataaaggat atgtttgcaa
     9061 tttggacact ttgcccacat gttatcagga acagatggcg agttagcagg tttgctctct
     9121 ctattaggat tgatccgaat atatttcttt ttcttaaaca gagccatcta tgcgcctcct
     9181 atctccgttt ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa
     9241 tcacctgcta tgactgcagg atgactaatc aaatccatct gaaattctgc gttcgtgatg
     9301 attccttctg tgataaattc acttaaggct cgctgcatct tcattaatgc ttcaaaacgc
     9361 gtatttccat ggacgattat ttttgcaatc attgaatcat aatatggcgg gatcgtatat
     9421 cccgggtaaa ctgcactgtc tactcgcagt cccatcccac ctgctggaag cagtaagttt
     9481 tggatcttcc ctggagaagg agcaaaatgg aaagcaggat tttctgcatt aatccggcat
     9541 tcgattgcgt gtccttggaa tactacttct tcttgacaca cattcaaagg ctcaccggct
     9601 gctatctcca cctgtttttt tactaaatcg attcctgtga ccatttctgt tactggatgt
     9661 tccacttgga ttcgagtatt catttccatg aaataaaaat cgccttcttc atccatcaga
     9721 aactcgatcg ttccggcatt ttcataattg actgctttag ctgctcgaac agcagcgtct
     9781 cctagtgctt ctctttttgt ttgtgagatc actacagaag gagattcttc cagtactttt
     9841 tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg accaaatcga
     9901 tcacctaaga tctgaacttc tatatggcga gcaggataaa tgattttttc caaatacata
     9961 tcatcatttc caaatgccgc tgctgcttct tgctgagctg aagaaaaatg ctgtgggagc
    10021 tcttcttttg attgaacttt ccgaatgcct ttcccgccac cacctgcagc tgctttcagc
    10081 atcactggat aaccaattct gtctgctatt tccaatgctt cttctactgt agataataca
    10141 ccagtacttc ctggaatgac tgggacattt gcttcttgca tcaattgacg cgcattgatc
    10201 ttatttccca ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat attgcactct
    10261 tcacacatgg aggcaaatcg gctattttca gataaaaaac caaatcctgg atgaattgct
    10321 tcggcttttg tcacgattgc agcgctcagt acttcctgaa cattcagata tgaatcaacg
    10381 gcttttgccg ggccaatgca gattgcttca tctgctaact gtgtatgaag tgcttcggca
    10441 tctgcttctg aatatatcgc tacggtttgg atccctagtt cgcggcatgc acgaatgata
    10501 cgtacagcga tttcaccgcg gttagctatc aatactttag aaaacatatt tcacctatcc
    10561 aatcataaag gttaattccg cttcggctac ttttttaccg tctactttag cgattccttt
    10621 gcctattccg gctgaagctt tcactttcaa tatctcaact tcaaggtata aggtatctcc
    10681 cggtgtaact ttcttacgga attttgcttt gtccaatccg ccgaaataag ccgtctttcc
    10741 cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct tgagccattg cttcaacaat
    10801 caatacccct ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat
    10861 cgttacattt ttcttagcaa cgatccgttc tccttcaacc atttcttcca ctcgatcaat
    10921 caaaagcatt ggataacgat gtggaatgat ttcttttatt tcttgtatgt tcattgtttt
    10981 ccctctttcc tgctttgctt atttttgctg aatcctaaag agtggctgtc catattcgac
    11041 aactgactcg ttttctacaa ggatttctgt gatcactccg tcggcctcac tagttatctc
    11101 attcatcagt ttcatcgctt caataatgca gataacttct cctttggcaa cttgatcacc
    11161 tacttttttg aaagctggtt tgtcgggcgc tggttgcaga taaacgattc ccacgattgg
    11221 cgaaacaacc tcgattccat caaatactgc cgattctttt tctttcatta tttctggagc
    11281 agttgttgct tgttcaggta tgacattgac tggctccgaa actggcaacg tttcgtttgt
    11341 ttgaaccata ttcatgcctc gacttgtttt atttttattc atatacaatt caaattgacc
    11401 ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat tgactgacta attctctgat
    11461 ttcattgata tccaaaatta ttcctcccag cgtttcatgc acagaaccgc attatgtccg
    11521 ccaaacccta atgaattact cattgcataa gtaaaacttg caggtttgct ttcgtttttg
    11581 acaactgtca agtcaatctc aggatcttgt ttttctacat tgatcgtagg tgggataaat
    11641 tgatgttcta atgccagcaa agttgccaag gcttcgatcc cgccggctgc tcccaataaa
    11701 tgtccagtca tcgatttcgt gctgctgacg taagtatctc tgaaattttc acctaatgcg
    11761 atttggattg cttttgcttc tgcctggtca ttggctggtg tacttgttcc gtgagcattg
    11821 atatatccga cttgttccgg ctgtacacca gcttcttcca tcgcaagctt cattgcttta
    11881 gatgcaccag aaccatctgg agtaggagct gtcatatggt aagcgtcaca gtttgcaccg
    11941 tatccaacga tttctccata gattttggca ccgcgagctt gtgcatgttc taaagattca
    12001 agaactagaa cgcctgctcc ttcacccatc acaaaaccat tccgattttc gtcaaacgga
    12061 atcgaaccac gattaggatc agttgattct gagagtgctg ttaaagctgc aaatccagca
    12121 atccccatct cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc agaatagcca
    12181 tgtttgatgt tacggaatgc ttccccaatt gaattcgttc cgctagcaca agctgttacg
    12241 atagctgtac aaataccttt tgcacctaca cgcaaagcaa tatttccagc tgccatgttg
    12301 gcaatcgcca ttggtacgaa cattggagaa actcgttttg cacctttttc atgtagacga
    12361 ataacttggt tttcgattgt tggaagacca ccgatcccag atccgatgat gacgccaaac
    12421 cggtcttgat tcattttttc tttatctaaa ccgctcatct ccattgcttc taaagcagcg
    12481 tagattccat agatcgaaaa catatccatt cgtttgctat cttttttaat aaaatattta
    12541 tcgtaaggga aatctttgac ttcacccgct aaggtaatcc ctgtttctga agcatcaaat
    12601 ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg
    12661 tcatttccaa taggtgaagt gactccatag ccagtaatta ccactcgatt catttgtttg
    12721 ctctcctttt cttatccgtg catgaccaat ccgccatcga cattgatgac ttgtccggtg
    12781 atatatggat tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa
    12841 cgctgcatcg gaatttgctt ttccgcttgt tccttgactt tgtctgctaa aacttccgtc
    12901 atatcagttg tgatgaatcc tggcgcaatg gcattacatg taatgcctct agttgcagct
    12961 tctcgtgcca cagattttgt taatcctact acgccagctt tactagctgc ataatttgct
    13021 tgaccaatat ttccaatcaa cccagataca ctagagagat taatgatggc cccttctcgc
    13081 tgcttcatca tttttttcaa tacgtgctgc gtcatattga aggttccaat caaattgata
    13141 tccagacatt ttttgaaatc ttctgcatcc attcgcatca caagtttgtc atttgtgatt
    13201 ccggcattgt ttactaacac atggatcgat ccaagctttt cttctgcttc tttgatcatt
    13261 tgtcccgctt tctcataatc agagatgtca ccggaaactc ctacgcattt tacaccgaat
    13321 gcttcgattt cttctatctt ttcttttgga atctctccgc gaccattcaa aatgatattt
    13381 gctccagctt ttgcaaaagc taaggccatc gctttgccga ttcctcttgt actacctgtg
    13441 ataaatacat tttttccttt tacatccata acgttcctcc ttatagtatt gctattgttt
    13501 catcaaatgt ctgcttattc tcaacacgca gaactggtat tgttttatcg atttttttca
    13561 taaatccgct taatactttt ccggggccga cttcgatgac ttgttcgatg ccgattgttt
    13621 ttaacttatg gatactctca tagaaacgaa caggttgcat gacttgctgt tcaagtagtc
    13681 ccgcgattgt ttctttcttc attatttccg ttgttgtgtt gcttatgata ggaaaagaag
    13741 gttcagagaa ttgaatctga cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa
    13801 tagctgtatg aaaaggcccg ctgacattta gagggatcat tcttttgaac cctttttctt
    13861 ttaatagtaa aacagcttcg tctactgctt tttcttcgcc accaatcacg atctgctgag
    13921 gtgtattata atttgcagga gatacgatgc cgtacttgct agcttcatga cagctttcct
    13981 cgatcgtttc aatcggcgca ttcatcactg caaccatttt cccgcttcca gctggggcag
    14041 cttctgtcat ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg
    14101 cattactagc tactaatgcc gaatattctc ctaaactcag tcctaaagct gcatctggga
    14161 tgattccatg ctccttcaat aagcgataat acgcgatgct gactgttaga atagctggct
    14221 gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca gccatatcgt
    14281 agccgagaat ctcacttgct tcatcaaatg tctgctttac aacggcttct tcatataaat
    14341 cttttcccat accttgatat tgtgcacctt ggccactaaa taagaatgct gttttcatgt
    14401 ttttctccaa tatatccaga atatatgttt gattcttcct attcaaaaag gccgagacat
    14461 tcctatttca ggaaatgtcc cagcccccaa cctaatttta atgattatgc agtttgtttt
    14521 tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc gattttcacg
    14581 tcaaattcat cttcaatttc attgatgatt tggaataaat ccaagctgtc agcttctaag
    14641 tcttcttgga tgtcagtagt caatttgatt tcttcagttt cttttcctaa ttcttctgcg
    14701 atgatttctt g
//
LOCUS       DAYYKN010000067        14439 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000067 DAYYKN010000000
VERSION     DAYYKN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.067"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..1115)
                     /locus_tag="ACHBB7_001733"
     CDS             complement(228..1115)
                     /locus_tag="ACHBB7_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9209684.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="ACHBB7_001734"
     CDS             complement(1112..1981)
                     /locus_tag="ACHBB7_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9209685.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="ACHBB7_001735"
     CDS             complement(2118..3404)
                     /locus_tag="ACHBB7_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9209686.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3677..4255
                     /locus_tag="ACHBB7_001736"
     CDS             3677..4255
                     /locus_tag="ACHBB7_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9209687.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4255..5184
                     /locus_tag="ACHBB7_001737"
     CDS             4255..5184
                     /locus_tag="ACHBB7_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9209688.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5181..6917
                     /locus_tag="ACHBB7_001738"
     CDS             5181..6917
                     /locus_tag="ACHBB7_001738"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9209689.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACHBB7_001739"
     CDS             complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACHBB7_001739"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM9209690.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7813..8322)
                     /locus_tag="ACHBB7_001740"
     CDS             complement(7813..8322)
                     /locus_tag="ACHBB7_001740"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM9209691.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACHBB7_001741"
     CDS             complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACHBB7_001741"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM9209692.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9623..10714)
                     /locus_tag="ACHBB7_001742"
     CDS             complement(9623..10714)
                     /locus_tag="ACHBB7_001742"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM9209693.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACHBB7_001743"
     CDS             complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACHBB7_001743"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM9209694.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACHBB7_001744"
     CDS             complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACHBB7_001744"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM9209695.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACHBB7_001745"
     CDS             complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACHBB7_001745"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM9209696.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4112 a         3144 c         2609 g         4574 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
     3601 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
     3661 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
     3721 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
     3781 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
     3841 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
     3901 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
     3961 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
     4021 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
     4081 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
     4141 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
     4201 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
     4261 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
     4321 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
     4381 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
     4441 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
     4501 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     4561 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     4621 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     4681 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     4741 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     4801 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     4861 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     4921 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
     4981 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
     5041 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
     5101 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
     5161 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
     5221 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
     5281 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
     5341 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
     5401 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
     5461 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
     5521 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
     5581 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
     5641 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
     5701 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
     5761 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
     5821 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
     5881 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
     5941 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
     6001 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
     6061 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
     6121 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
     6181 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
     6241 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
     6301 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
     6361 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
     6421 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
     6481 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
     6541 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
     6601 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
     6661 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
     6721 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
     6781 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
     6841 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
     6901 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
     6961 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
     7021 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
     7081 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
     7141 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
     7201 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
     7261 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
     7321 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
     7381 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
     7441 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
     7501 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
     7561 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
     7621 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
     7681 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
     7741 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
     7801 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
     7861 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
     7921 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
     7981 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
     8041 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
     8101 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
     8161 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
     8221 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
     8281 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
     8341 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
     8401 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
     8461 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
     8521 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
     8581 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
     8641 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
     8701 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
     8761 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
     8821 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
     8881 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
     8941 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
     9001 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
     9061 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
     9121 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
     9181 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
     9241 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
     9301 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
     9361 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
     9421 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
     9481 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
     9541 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
     9601 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
     9661 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
     9721 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
     9781 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
     9841 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
     9901 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
     9961 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    10021 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    10081 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    10141 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    10201 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    10261 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    10321 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    10381 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    10441 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    10501 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    10561 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    10621 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    10681 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    10741 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    10801 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    10861 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    10921 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    10981 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    11041 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    11101 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    11161 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    11221 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    11281 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    11341 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    11401 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    11461 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    11521 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    11581 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    11641 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    11701 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    11761 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    11821 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    11881 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    11941 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    12001 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    12061 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    12121 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    12181 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    12241 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    12301 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    12361 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    12421 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    12481 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    12541 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    12601 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    12661 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    12721 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    12781 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    12841 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    12901 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    12961 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    13021 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    13081 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    13141 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    13201 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    13261 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    13321 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    13381 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    13441 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    13501 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    13561 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    13621 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    13681 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    13741 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    13801 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    13861 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    13921 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    13981 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    14041 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    14101 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    14161 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    14221 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    14281 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    14341 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    14401 aagttttcta gatttcataa aaaatcgctc caaactgat
//
LOCUS       DAYYKN010000068        14110 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000068 DAYYKN010000000
VERSION     DAYYKN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.068"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            279..1127
                     /locus_tag="ACHBB7_001746"
     CDS             279..1127
                     /locus_tag="ACHBB7_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM9209697.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="ACHBB7_001747"
     CDS             complement(1298..1639)
                     /locus_tag="ACHBB7_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFM9209698.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="ACHBB7_001748"
     CDS             1725..2432
                     /locus_tag="ACHBB7_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM9209699.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="ACHBB7_001749"
     CDS             2419..2742
                     /locus_tag="ACHBB7_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM9209700.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3868)
                     /locus_tag="ACHBB7_001750"
     CDS             complement(2831..3868)
                     /locus_tag="ACHBB7_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9209701.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4315..5298)
                     /locus_tag="ACHBB7_001751"
     CDS             complement(4315..5298)
                     /locus_tag="ACHBB7_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM9209702.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5714..6484)
                     /locus_tag="ACHBB7_001752"
     CDS             complement(5714..6484)
                     /locus_tag="ACHBB7_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9209703.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6477..7412)
                     /locus_tag="ACHBB7_001753"
     CDS             complement(6477..7412)
                     /locus_tag="ACHBB7_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209704.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8006..9655)
                     /locus_tag="ACHBB7_001754"
     CDS             complement(8006..9655)
                     /locus_tag="ACHBB7_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM9209705.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9850..10746)
                     /locus_tag="ACHBB7_001755"
     CDS             complement(9850..10746)
                     /locus_tag="ACHBB7_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9209706.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10739..11419)
                     /locus_tag="ACHBB7_001756"
     CDS             complement(10739..11419)
                     /locus_tag="ACHBB7_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9209707.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11420..12202)
                     /locus_tag="ACHBB7_001757"
     CDS             complement(11420..12202)
                     /locus_tag="ACHBB7_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9209708.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12195..13112)
                     /locus_tag="ACHBB7_001758"
     CDS             complement(12195..13112)
                     /locus_tag="ACHBB7_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9209709.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13115..13399)
                     /locus_tag="ACHBB7_001759"
     CDS             complement(13115..13399)
                     /locus_tag="ACHBB7_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209710.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT         4176 a         3013 c         2456 g         4465 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat
     3601 cgccgacaga aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa
     3661 aacctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     3721 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     3781 gttctttttc atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga
     3841 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     3901 catccataca gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc
     3961 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     4021 tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc
     4081 acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     4141 agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg
     4201 gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac
     4261 ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     4321 ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc
     4381 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     4441 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     4501 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     4561 cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc
     4621 aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat
     4681 cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc
     4741 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     4801 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     4861 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc
     4921 cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     4981 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5041 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5101 tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5161 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     5221 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     5281 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     5341 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     5401 aaaagatgtt tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt
     5461 ttccggaata aatggtgttc aaaagcttgc atctgcggta ataagtccaa ccaaacgaaa
     5521 aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
     5581 caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattcgctc aaaaaacaac
     5641 aaaatatgag gaactatttt cccgtttgtt tctctaatac tcaggtcaaa acgctgattt
     5701 cacaacctct tttctaactt ctcaatcctc ttcctttatt gatcaaatac caagtaacgg
     5761 cgtacaaaac aacgatgaat aacacaagta cgatcatcga tgttgctact ggcacatcga
     5821 tcgttccaag gaatccgtaa cggaaaccgg agatcatata aacgatcgga ttgacttttg
     5881 aaacagcttg ccaaaagggc ggtaacatag agatggcata gaaaacgcca cctagataag
     5941 ttaacggctg caagacaaat gtaggcacga tcgatacatc atcataagac tgagcaaaaa
     6001 tcccgttgag cagtcctgct aatgaaaaca agattgccgt catcaataat gtaatgatga
     6061 ccatgaacca tgaatacaca tgaagtggta caaagaacaa ggaaatgatc gttacgagag
     6121 agccgacgag aatactgcga cccacaccac caatcacaaa gccccaaata atcacatgcg
     6181 taggaacagg tgctaccaag atttcttcga tatttttctg aaatttttga gagaagaagg
     6241 acgaagaaac attggcatac gaactagtga tagcagacat cataatcaaa ccaggaacga
     6301 tgaattccat atatgaaaaa ccacccatgt caccgatccg cccaccgatc atttttccga
     6361 aaataacaaa atacaaggat gtcgtgatca ctggcggaac cagtgtttgg acccatatac
     6421 gcaaatagcg gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa taaagattaa
     6481 acatttttct cctccgtatg attattttct tctgtgattt tcaagaacag ttcttctaaa
     6541 cgattcgact tattcctcat agaaagaact ttgattcctt gtcttgtaag ctgatcgaag
     6601 attccgttca ccccttgatt tcgttcaact tcgacagcga ttgtctgctc atcttcaaat
     6661 gtacaagcat atccttcaat cacaggtttg gtatcatatg gcgctaaatc aaagataaat
     6721 gtttcaaatt gcagttttga cagaagctct ttcatacttg tattctcgat cagttcaccc
     6781 gattggatga ttccgatgtt gcgacaaagc atttcagctt cttctagata atgcgtcgtc
     6841 aaaatgatcg ttgtaccatt ttcgttcaat tctcgtagaa actcccacat ctcgcgtctc
     6901 aactcgatgt caactcctgc tgttggttca tccaagatca gcaaacgtgg ttcatgcatc
     6961 aatgccctag caatcatcag tcttcgcttc attcccccgg aaagcatacg tgcacggaca
     7021 tttctctttt cccaaagatt ggactgcttc aaatattttt cgctgcgttt catcgcttct
     7081 ttgcgagaga caccgtagta gcctgcttga ttcacgacga tttgctggac cgtttcaaaa
     7141 gggttaaaat tgaactcttg tggaaccaat ccaatctgtt gctttgctcg aactagatcc
     7201 ttgtccagat cataaccgaa gacattcact tttccagatg ttttattgac taaagaagta
     7261 ataatcccga tcgttgtaga cttcccagct ccatttggcc ctaatagcgc atagaaatct
     7321 ccttctttca ccgaaagatc gattccccgc aatgcttcga ccccacttcc atagatcttt
     7381 ttcaaattat ttatttcaag cgcatttgcc atttatttcc tatctcctta tagacatgat
     7441 tcttaattgt atcatggaat acagcagatg aaaaaacaac ttggcttcca cacaaaaaca
     7501 cacctgctaa caaagaggag atgtgttttc tagttcatga tcatcttcga tagaataact
     7561 atctaaacaa ctctggaact tttaaaatca taaaagtacc gacagcaact actacgatga
     7621 catatagaag atccttataa ctgaggttct tgtctttttt attcatgtta agctttcctc
     7681 cgtcctttta tcgaaagctt ttttctttta ttttaaccga aataaataaa atggaaatag
     7741 ttattttcaa tttctgtttt cgctgtttgt tttacggaga actttgccat ggaacgtgcg
     7801 tctcgtcgct gatgctaagt tttttcgata aaagctgcta gaaggaagac agcgtggttt
     7861 attttgtatc gtcttttgct aaatcttcca agatcatctg gacagcagac tcgttcccat
     7921 tgaattctgc cacttttttg gcatcaatgt acttcttttg ataatcgtct ttttggaccc
     7981 acgcatcatg cttcgtttga taaagttaat caaacggata ccactgacgc tgttcatcga
     8041 acaacaaact ttctttttgg gcctgttcaa ctatggattt aggataattc aaaactttca
     8101 gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga
     8161 ttccttttcc ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa
     8221 tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc
     8281 accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt atttgttcct tttaaaatct
     8341 catctacgaa gcagtagcag cgttctttgg aagccacttg gtccagcaaa cgtttgatcg
     8401 atttgatttc tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg
     8461 aagtcaacac atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct
     8521 gtgctagaat acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg
     8581 aacccgtgac taatgtattc ttctgccagt tgactggatt aactaccgct tcttttaaca
     8641 atggatgata gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga
     8701 tcggcatata ggtgcgataa ttcaaaacgg ctgctgcaac ttccaattct cccagcaatt
     8761 cccacagctt gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg
     8821 agataaatgg cagcatgaac atgatgttga catattcaaa aatcaattct gcttcactcc
     8881 cgctcttcat tcgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct
     8941 gtttcagctc tttttgaagt ggtgtcgtca tttttgctaa ctgattggcg gctgaaatcg
     9001 ttgaaaccaa ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat
     9061 aatagaccgt attgaatact aagccaccga ttgctagata gattcctagt acttgcccta
     9121 aaagaagcaa acctacaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc
     9181 gtccaatctt tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt
     9241 ttcctaaacg agcgaattga tattggattc tttctctcag ttgaggattt tcctgataaa
     9301 aagcaattag tttttctaac tgataatctt ctccaaaatc aaagctacgt aaacgctgat
     9361 acaacgcttc tgaacctaca ctagaatagg tactgttgat cccttcaaat acttcgaaca
     9421 tatccaagtc ataccaagta agatcgtcga tttcactatc ccatgaacgg aattttttct
     9481 ccgtgagcca cgcatccttc aaactttctt ctttatcaaa tctcggttga taaggaattt
     9541 ttccccaatt actacgaact gtttccttta ctttcaaacg tgaaaaaatg tctaaaacaa
     9601 ctaataaaat aacaactgca ataataatta aaacgataaa ttctctttgg cccatatccc
     9661 tctccgttcc ttgatttgtt agttttagta tagcactgtt gcgctatttg aaaagggggt
     9721 acaaaaataa ctgtcttcgt tacctaattc gtatcaatca caccaaaata aaaacacctt
     9781 ccaaaaaaga agatgtcttt aaaacgagat acaatttttt gtcattcact tcaggtaaat
     9841 tagagccaat taaaccctcc gcaacttgac ggagatggag aagatgcccc cgtctaatct
     9901 agcggtcatc tctcctccca ttccttccac caattttttt gacaggtaaa gcccgattcc
     9961 gctagattcc gtatttgtgc ggctggtatt ttcggtataa aaacgctgga agactttttc
    10021 aacagaggtt tgcgctcctg gtttgatagg attggccact gttaatcgaa tgaatgaatc
    10081 ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa cgcaataaat tctgtatgat
    10141 attttgcagc actcgcagca acatgagcgg atctgctaat acagttgtat ttatcgagtg
    10201 ttcagcgaac gctacctcga tatttttctc agaaaattgt tcataaaaag agaaaaatac
    10261 ttcttccacc tctttagaaa aagaaagcgt atggagatcc atttcttttt gcttcgtctc
    10321 tattcgagca agatcataaa actgatccgt catactgagt aatcttgcaa cagattgttt
    10381 gatgatcgtt aaatacttct cccgtttctc cgtatcctct acctgttcca gtaattgtac
    10441 ataaccgtta atagcagtca aaggagttct gagatcatga gaaaggccga cgatcgatag
    10501 tctgatgttt tcttccattt cttgaaatgc ttgtcctttt tgttcatttt catccaccca
    10561 tgtattcagt gcatctacta ggtccattag attcttttct cgaaattcga ctgaaagacg
    10621 accaccatat tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc tcttgatttc
    10681 cctttgcagg aagaaaataa aacttcccat aaagataaca acgagaagca acagccactt
    10741 aatcaaatct aaaccccact ccccacaccg tatgaatata cacagcatgt gcctttgcta
    10801 gtttccctcg aagattggaa atatgcatat tcactgtttt ctcactatca aaatatggtt
    10861 cttgccaaac tgtttcataa agatttgctc gcgaaaatac ttttttcgga tgttctaaaa
    10921 aaagcttcag cagatcgaat tctcttcctg tcaaatggac agccacacca ttcacattta
    10981 catccctcga ctctttgtcc agtaagattt ccttgtaata gatgcgttgc gacagatctt
    11041 ttacttggac agaagtctga tgacgaagtt ggatgccgat tcgtaacaac agttcttcga
    11101 tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa actttatctc
    11161 tttgatcgct ttttgctgaa agaacgatga tcggaacatt cgatgtctgt cggatttctg
    11221 ctaacaatgc ttcacctgaa agccctggaa gcatgagatc tagaaggatc aaatcgacag
    11281 gatatgtgct taacaataat tttccttccg tacctgaata agcatcaaga agtttgtatc
    11341 tctgttcaag aacctcctcc agtaaagaat gaacagcttc atcgtcttcg ataatcaaaa
    11401 tagtttccag caggaacacc tacttaatgt cagatttttg gaaaataaac attcctagag
    11461 ctgatgatac cacgattgtt actagactga ccagaatcat tttcggtgct tgcgaataag
    11521 ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac tccgttagaa
    11581 attccaagcc tttgattttt gcgaaaacac cgggaacgaa ggccgttccc atgatataaa
    11641 gaaaagaaag aagcaaactg acactggtac ttctagtgat gaatgtcaaa gccactggaa
    11701 cagataatgt cgctaatatc agcagaagca atacaggaaa tacttttaga acataatttc
    11761 catagttttt gattccctca acttcaccaa atccgaaagc aagtgtgtaa gcaatcatgg
    11821 ctagccccgt aatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg
    11881 acacataata agtcattcga ttccgtccta aagataacgt attgcgaatc gtaccgtttt
    11941 gaaattcttc cccaatataa aagcacgcaa atgctgcgaa agccagaaga atccatgcag
    12001 ccattttttc caccattaaa atccctgttg caccgttgat gactgtatcc gttgcttggt
    12061 ttaattcatt gccgtatttt gtatgcatat accattcgtt ccaagaataa aaaagcggaa
    12121 gtacgaccat cagtcctagt gagacccaca ttttacggct cttccatact tttatttttt
    12181 cagcagttaa tagattaaac atgtggattg cctccagtta attgtaagaa ataatcttcc
    12241 agtttttgcc caactaggtg gataccatcc acaggaactt ttgcctctac caatgcttga
    12301 ttgatggctg ctacttggtc aattccttca aaaagctgga taacacggct agattggaca
    12361 aaataatccc tataaccaag agagtcgaga accgttactg cttgttccgt cgccgaagtg
    12421 tcaattttta taaattgacg agattcttgt gccaattctt ctttcgataa ttctttgatc
    12481 aagtgtcctt catgcaaaat accgtagtga gtggcgatct gtgacaattc atctagcaag
    12541 tggctagaaa gcaagacggt gatgccccgt tcttttacca aacgttggat gatttctcgc
    12601 atttctgcca tccctgctgg atccatccca ttcgtcggtt catccaaaat caaaaattct
    12661 ggattcgtga tcagtgcatt tgcaatagct aaacgctggc gcatacctaa agagaaattc
    12721 tttgctttct ttttccctgt attttccaat cgcatcattt tcagcaggtc ttcgatttct
    12781 ctatccgaca caccgccgtt cgctgcttgg atacgtaggt tatctcttgc agttaattct
    12841 ggataaagag caggggtttc aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc
    12901 gtcgttccaa ataattcgat cgaaccttca tcaatcgaga tcaaacccat aatagttctc
    12961 ataaaggtag acttacccgc tccattctcc ccaatcaaac catagatcat tccacgtttg
    13021 atttcgatcg atactttatc taatgctttg gcagcaccgt attttttggt tataccagtt
    13081 gctttcaaga ccgtttcatt tccaaaatcc atacttatcc ttctttctta ttgataagtg
    13141 aagtatagtt gacgggtata aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt
    13201 ccaattgaat ttcaagaaaa atcagactgt ctcgcagacg tggttgagtt gtctgccaat
    13261 caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta
    13321 tcgttgacat cagacgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat
    13381 aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt
    13441 ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct
    13501 tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca
    13561 aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca
    13621 caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt
    13681 tatagatcga atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa
    13741 aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct
    13801 cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt
    13861 aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggtttt ttcttcgtat
    13921 cattgaataa gtttgaaaag ccttgattta taaaggtttt tccgtaaata tattttgtta
    13981 tatttgatta aatttgaaat gtttgtgttc taaaatgtgt tctaatttaa aggcagtata
    14041 tttcgtattt tatattaaat ttttaggctc attcattctc aaatccattc tatttagtca
    14101 ataaatattt
//
LOCUS       DAYYKN010000069        13754 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000069 DAYYKN010000000
VERSION     DAYYKN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.069"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..372
                     /gene="yaaA"
                     /locus_tag="ACHBB7_001760"
     CDS             130..372
                     /gene="yaaA"
                     /locus_tag="ACHBB7_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM9209711.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            359..1483
                     /gene="recF"
                     /locus_tag="ACHBB7_001761"
     CDS             359..1483
                     /gene="recF"
                     /locus_tag="ACHBB7_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM9209712.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1480..3426
                     /gene="gyrB"
                     /locus_tag="ACHBB7_001762"
     CDS             1480..3426
                     /gene="gyrB"
                     /locus_tag="ACHBB7_001762"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM9209713.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3446..5917
                     /gene="gyrA"
                     /locus_tag="ACHBB7_001763"
     CDS             3446..5917
                     /gene="gyrA"
                     /locus_tag="ACHBB7_001763"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM9209714.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6153..6452
                     /gene="rpsF"
                     /locus_tag="ACHBB7_001764"
     CDS             6153..6452
                     /gene="rpsF"
                     /locus_tag="ACHBB7_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM9209715.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6502..7014
                     /gene="ssb"
                     /locus_tag="ACHBB7_001765"
     CDS             6502..7014
                     /gene="ssb"
                     /locus_tag="ACHBB7_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9209716.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7040..7276
                     /gene="rpsR"
                     /locus_tag="ACHBB7_001766"
     CDS             7040..7276
                     /gene="rpsR"
                     /locus_tag="ACHBB7_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM9209717.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7419..9392
                     /locus_tag="ACHBB7_001767"
     CDS             7419..9392
                     /locus_tag="ACHBB7_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM9209718.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9398..9850
                     /gene="rplI"
                     /locus_tag="ACHBB7_001768"
     CDS             9398..9850
                     /gene="rplI"
                     /locus_tag="ACHBB7_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM9209719.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10124..11491
                     /gene="dnaB"
                     /locus_tag="ACHBB7_001769"
     CDS             10124..11491
                     /gene="dnaB"
                     /locus_tag="ACHBB7_001769"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM9209720.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12271..13563
                     /locus_tag="ACHBB7_001770"
     CDS             12271..13563
                     /locus_tag="ACHBB7_001770"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM9209721.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4581 a         2478 c         3023 g         3672 t
ORIGIN      
        1 atttttgttt aaatcacctt ttttagatta aatcgtctta tatggaaaaa tatatggata
       61 aatttgtttt attgcgaaaa aaaaggtata atagagtata cttttaataa tgaaaatgag
      121 ggatgggcat tgaaacaaca agtcattcta aatacggaat tcatgacgtt gggccaaatg
      181 ttgaaagaag tcacagttat cggtagtggc ggacaagcaa aatggtattt ggcagaaaac
      241 actgtgttag tcgatggcga accagaaaat cgtcgtggac gcaaacttta tcccggcatg
      301 atggttgaag ttccagaagt aggaactttt tttatggtaa aaaaaggaaa cgacgataat
      361 gaggctgaat aagctgtatt taaaaaatta tcgaaattac gaagagctga acatcgaatt
      421 ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga
      481 aagtatctac gtattagcta tgacgcgaag tcatcggaca agtagtgagc aggaattgat
      541 tggctggact gacgatcagg cgatgattca aggagagatc acaaaaggtt catccactct
      601 tccattagaa atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca
      661 aaaaaagtta agtacttacg tgggacaaat gaatgtcatt ttatttgcac cagaagatct
      721 ttctttagta aaaggcagcc cgcagatcag gcggaagttt ttagatatgg aattaggcca
      781 gatcaatcca atctatcttt atgatcttgt ccaataccaa gcaattttga aacaaagaaa
      841 ccagtattta aagcaattga atgaaaaaaa acaaactgat ttgctttatt tggatatttt
      901 gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa
      961 gcgattggag ttttgggcta attctttgca tcagcaaatc acccatcaaa aagagcagct
     1021 ggaaatcgag tatctcaccg cagtcgattc tttagaaaca catacgcaag agcaaatcca
     1081 agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac
     1141 gacagttggt cctcatcgtg acgatctttc cttttttatt aatcagaaaa atgtgcagac
     1201 ttatggatcg caaggacagc aaagaacaac tgcactaagt gtcaaattag cagaaatcga
     1261 tttgattaaa gaagagacgg gggaatatcc aatccttttg ttagatgatg tgatgagcga
     1321 gctggatgat agtcgtcaac tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt
     1381 cttaacaaca acgacacttg accatgtaaa aaataaaatg actgttgaac ctgagatttt
     1441 ttatgttcaa caagggaaac taaaaggaga agaaactgaa tgacagaaga aagaagttta
     1501 gtagaacgcg ctaaagagta tgatgccagt cagattcaag tacttgaagg acttgaagcc
     1561 gttcgtaaac gacctggcat gtatatcggt tcgactagtt ctgagggtct gcatcacctt
     1621 gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag caggttttgc aacgaaaatc
     1681 catgtgatta ttgaaaaaga caacagtatc accgttattg acgacggtcg ggggatacct
     1741 gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa cggtctttac tgttttacat
     1801 gctggaggaa aattcggcgg tggtgggtac aaagtttctg gtggacttca cggagtagga
     1861 tcttctgttg ttaacgcgct ttctacacag cttgatgtca aagtatacaa agaaggaaat
     1921 gtgtactatc aagaatatcg tcgtggagct gtagtggacg acttaaagat tatcgaacaa
     1981 acggatcgcc atggaacaac ggttcacttt acgccagacc ctgaaatttt tacagagaca
     2041 actgagtttg atttttcaaa attggctacc cggatccgtg aacttgcttt cttgaaccgc
     2101 gggatgcgta tatcgattga agacaaacga gaagaagaac cagtcatcaa tgaataccat
     2161 tatgacggcg ggattaaaag ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt
     2221 ccagaacctg tatatttgga aggtgaacag caagatatcg cagttgaagt ctctatgcaa
     2281 tatacagacg gctaccattc caatatcatg agttttgcca acaatatcca tacgtatgaa
     2341 ggcggaacac atgaatcagg attcaagact gctttaactc gtgtgataaa tgattatgcc
     2401 cgaaaacaaa agctgatgaa agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa
     2461 ggattgacag ctgttatttc tatcaagcat cctgaccctc aattcgaagg acaaacaaaa
     2521 acaaaattgg gcaattccga agtacggaca gtcactgatc ggttatttag cgaacatttc
     2581 atgaaattct tgctggaaaa cccaagtgtg ggccgtcaaa ttgttgaaaa aggattacta
     2641 gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct
     2701 ttagagatta gtaatctgcc aggaaaatta gcggactgct ctagcaacga tcctgaaaaa
     2761 tgcgaattgt tcatcgtcga aggagattcg gccggcggat cagctaagca aggtcgtaat
     2821 cgagagtttc aagccatctt gcctattcgt gggaaaatct tgaatgttga aaaagcaagc
     2881 atggacaaga tcttggcaaa tgaagagatc cggtctttat ttactgctat gggtacaggg
     2941 tttggagaag attttgatgt atcgaaagct cgctaccaca aattagttat catgactgat
     3001 gccgatgtcg acggcgcgca tatccgtaca ttgctgctga ctctttttta tcgttacatg
     3061 cgtcctattg ttgaagctgg atacgtctat attgctcagc cacctttata cggggtaaaa
     3121 caagggaaga atattacgta tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa
     3181 gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa aggacttgga
     3241 gaaatggatg atcatcaact atgggaaaca actatggacc caagcaatcg gatgatggcc
     3301 cgcgtcagtg tggacgatgc tattgcagca gatcagatat ttgaaatgtt gatgggggat
     3361 cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa gaacttagat
     3421 atttaatcat gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca tccatgatgt
     3481 caatctaacc agcgaaatga aagactcctt catcgattat gcgatgagtg ttattgtcgc
     3541 acgtgcactt cctgatgtca gagatggtct gaaacccgtt catcgccgta ttttatacgg
     3601 catgaacgaa ttaggtgtaa caccggataa agcccataaa aaatctgcca gaatcgtcgg
     3661 agatgtcatg ggtaaatacc atcctcatgg agactatgcg atctatgagt caatggtacg
     3721 gatggcacag ccattcagct accgttatat gctagtggat ggtcatggaa actttggttc
     3781 tgttgacggt gacggtgccg ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc
     3841 tacagaaatg ctgcgtgata tcaataagaa cactgttgat ttccaaagca actatgatga
     3901 tacagaaaaa gaaccagttg tactacctgc tcgcttcccg aatcttttag tgaacggaac
     3961 aacggggatc gccgtaggta tggccacgaa tatcccacct cataacttgt ctgaggtagt
     4021 agcggctatc gatttgctga tggataatcc tgatgtgacg accaatgaat tgatggaagt
     4081 attacctgga ccagattttc caacaggtgg tttggttatg ggaaaatcag gaatccgaag
     4141 agcgtatgaa acaggtaagg gttctattac cgtacgtgca aaagttgaga ttactgagat
     4201 gccaaatggt aaagaacgga tcatcgtgac agaattgcct tatatggtca ataaagcaaa
     4261 attgatcgaa cggatttctg aactgcacag agacaagcgt atcgaaggga tcacagattt
     4321 gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag
     4381 cgcatctgtt atcttgaata acttatataa attgacatcc ttgcaaaatt catttggatt
     4441 caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac aaattcttga
     4501 aaactatatc gaacatcagc gcgaagtcat cacaagacgg acagtctttg aaaaagaaaa
     4561 agcagaagca agggcacaca ttttagaagg tttgcgtatt gctttggatc atatcgatga
     4621 aatcattgcg attatccgcg ggtctaaatc agatgacgaa gcaaaagcga cgatgatcga
     4681 acggttcgat ttatccgatc gccagtctca agctatttta gatatgcgtt tacgccgatt
     4741 gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa aactgattga
     4801 agatttaact gacattctgg caagaccaga acgtgttaca gaaatcatca agaatgaatt
     4861 agcagacttg aatcaacgat ttggtgataa acgacgcaca gaattactag tcggtgaggt
     4921 cttaagtctg gaagatgaag atttaatcga agaagaagaa atcgttatta cattgacaaa
     4981 caatggttac atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa ggggtggacg
     5041 tggtgtccaa ggaatgggta tccacaatga cgacttcgtc aaaaatctgg tttcttgttc
     5101 tacccatgat acattgttat tctttacgaa taacggaaaa gtgtatcggg caaaaggata
     5161 cgaaattcca gaatacgggc gcacagccaa aggaatacca attatcaatt tactaggtat
     5221 cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag aagaaggcca
     5281 ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc
     5341 taacatccga agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa
     5401 tgtgctactg acagatggtc aatctaatat cattattgga acacataacg gctactctgt
     5461 cactttcaaa gaagaagcag tccgcgatat gggacgtaca gcttccgggg ttagaggaat
     5521 acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta atgaaggaag atcaagaagt
     5581 attagtgatc acagaaaaag gttacggaaa acggacaaaa gtatcggaat atccagtgaa
     5641 aggccgcgga ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg gtccactagc
     5701 tggattgacc actgtttctg gagatgaaga catcatgctg attacagata aaggtgtcat
     5761 tatccgtttc aacgtatcga ctgtttctca aactggacgt tcaacattag gcgtacgttt
     5821 gatgaagatg gaagctgata cgaaagttgt cacgatggca gcagtcgaac cagaagctgc
     5881 agaacaaaca gttgaagaac aaggatcaga agaatagcca tcaagactaa ttgtgtaagc
     5941 tagcaagaat gaccgattaa aaaatctggt tattcctgct ggcttttttg attagttgaa
     6001 tttctcttaa aatattcgaa actgttcatt ctgtattgct tttgaccaaa atagagtgta
     6061 taatcagagc ttgtgagtag tctaattaat tagataactc tccttgctct taggaaccta
     6121 agagccgaaa gtccacaggg aggtgaatat caatggaaaa cacgaaatat gaaatcatgt
     6181 acattattcg tccaaacatt gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg
     6241 caatcttgaa agataacgga gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt
     6301 tcgcttatga aatgaacggt taccgtgaag gtatctatca catcgttaaa gtatcttctc
     6361 catcatcagc aaatgctgtc aatgaatttg accgtcttgc taaaatcaac gacgatatca
     6421 ttcgtcacat gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa
     6481 cgatgagagg agatgactat tttgattaat aatgttgtat tagttggacg tctgacgaaa
     6541 gatccagatt tgcgttacac agcaagtggt acggcagtcg caacatttac tttggctgtg
     6601 aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt
     6661 tggcgcaaat cagcagaaac tttagcaaac tatgctcgta aaggaacact tttaggtgtt
     6721 actggacgta tccaaactcg ttcttatgat aatcagcaag gacaacgtgt atacgtgaca
     6781 gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa
     6841 caaagcggcg gattccaaag ttctggtcaa tcagctggcg gatttggcgg aaacaataac
     6901 tctaatcaaa catctcaatc atccaacggt atgcctgatt tcgaccgtga tacttctgac
     6961 ccattcggct caagttcaac gatcgacatt tcagacgatg atttaccatt ctaaacgaat
     7021 ttgataggag ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt
     7081 agactacatt gctgctaacc atattgaata tatcgattat aaagatatcg aattactaaa
     7141 aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta caggcgctaa
     7201 aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt
     7261 cgttagcgaa gactaataaa tcctaggaat tgaatgactg atccttttga tcagctattc
     7321 aattcctttt tttctgtaaa aataacctct attcgtttag tcgctcgaat gacgaaagtt
     7381 atggtaaaat caaattagtc atatatggga ggaaaattat ggaaaataaa aacaatcatt
     7441 tatccccgaa acctctcttc cttatcctgt ttatattaat cgaagtattg gtagtcttaa
     7501 ttttaccatt tcgttttgtt agcgcttcgg tcattctatt gatgaattta tggggtgttc
     7561 gctattggtt gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa atccagcagg
     7621 cttcgactgt agctgaaacc aatctagctt atgtctcaga agtcatgcct gtaggagtga
     7681 ttgcttatca gccatcaagc caaaaaatcg aatggctcaa tccattagca ttgaaaatca
     7741 aagatcaaag tggattaaca gaggaagaat tgctagatac tttttttact gcaaaagaaa
     7801 aaaagaaaaa ccaagtctat ctagcagata aaacatttca gtttgatatg gatacagtaa
     7861 agggtgttgt ttattttaat gatattaccc aaaaccagtt gttaaaactc catcagacga
     7921 acagtcagcc ggtgattggt attttatcta ttgataacta tgcagatgcc attgataaat
     7981 tggatgacaa agagatttct tatttgaact ctttcgtcac tacactagtt tctgactgga
     8041 tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac
     8101 agatgatcga tctgaaaaag atgatggata aaaaatttga tttgctggat cgctttcgag
     8161 cagaagctga aaaacagagc tcgcctatca cccttagtat ggggattgct tatggctcaa
     8221 gaaccctcga tgaaatcggt ggagaagctc agaataattt agatgttgct ttagtccgtg
     8281 gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt tattatgggg
     8341 ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt
     8401 tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat ggggcacaag tttccggata
     8461 tggatgcttt aggctctgct tttggtgtca gttgtctggc acgcttcaac cagaaaaaag
     8521 cttacatcgt tatcaacgag aacgagatca ttccagatgt agaacggtgt cttgaagaaa
     8581 tccataagca tcctgaatta gaaatgcaac taatctctcc agaacgtgcc atagagctga
     8641 aaaaagaaga ggacctcctc gtgatggtgg attaccataa accttctcta tcgatctctc
     8701 agggcctata tgagcaattt gataagattg tgatcattga tcaccatcgt cgtggggaag
     8761 agtttccaag taaacctctg ctgacttata tcgaatcttc agcttcttca gcttcagagc
     8821 tagtttctga gctgatccaa taccaaagca gcaaaacaaa tcgcttagat cgttttgagg
     8881 ccacattatt gcttgcaggg atggttgtag atacaaatag tttcaatgtc cgtacaactg
     8941 cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg agcagacgca tccttagtac
     9001 aatatttatt gagctcggat ttagcgacat acttggagat cagcaatttg attgctggaa
     9061 gcgactatgt gacttctgat attgttgtgg cggcagggac agacgaaaaa gagtataata
     9121 gtgttatagc agcaaaaaca gcagatacat tgctttcgat gatcaatatc aatgcagctt
     9181 tcgtcattac aaagcggaca gacggcttga ttgggatcag tgccagaagt accggcacga
     9241 tcaacgtcca aatcatcatg gaatccttag gtggcggagg acacttcaca aacgcagcta
     9301 ctcaactgga aaatacgaca gttgaggaaa caaaacagaa gctatttgct gcaatcaaag
     9361 aaaatatgaa tcaaatatat gataaggagt aatccaaatg aaagtaattt ttttacaaga
     9421 tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt atgcacaaaa
     9481 ttatctaatc aaaaataact tggcaaaaga agcaacgaaa agcagtatca gtcagttagc
     9541 aggacaaaag aaagcacaag aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt
     9601 aaaagaattc ttggaaaaag aagatactgt agtcgaattg aaggcgaaag caggggaaga
     9661 cagccgtttg tttggttcga tcccttcaaa acaaattgct gaagggttaa acaaacaata
     9721 caaaatcaaa ttagataaac gaaaaatcga attagaaaat ccgatccgtt cactaggcta
     9781 cacaaacgta cctgttaagc ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt
     9841 acaagaataa caaatcttga tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa
     9901 gacaaaccga aatttatctg agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg
     9961 atttcagatt gatttcggtt tcttttttgg gaacagcaaa atagcataaa gaaaacgagt
    10021 ataataaaag agaactattt ttttattaca ctttcggtac gaatgatgac ttttctctca
    10081 ttttcttgta aaatgaagca attgaagaca aggagcgata cgtatgaatg aagtgtggca
    10141 ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg gttttaggct ctgtttttct
    10201 tgacgcagaa acgatcattg atgccatgga gtacatagag ccaaaagact tttatcgtcg
    10261 tggccatcaa attatttttg agacaatgat cgagttaaat gatcgtaatg aagcgattga
    10321 cgtggttact gtcaaagacc ggctggaaca agccaatttg ttagaagatg ccggtggttt
    10381 aagttatctc tcagacttgg cattagcagt tccgacagca gcaaatatcg tctactatgc
    10441 gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc cagacagcaa caggaatcgt
    10501 tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag
    10561 tatcttggag gtttcggaaa aaagaaatcg aagcggcttt ttatctattg ctgaagtact
    10621 gaactccacg attgaaaata tcgatcgact agctcaaaac aatgaagaaa tcacaggtct
    10681 accaactggc tatcaaggtc tggataaaat gacagcaggt ttccagcccg aagaactaat
    10741 cattcttgct gcacgtccag cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa
    10801 tgtaggaaca aaaacagatc gttctgttgc gatatttagt ttggaaatgg gggcagaatc
    10861 tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag gccagccatt tacgtacagg
    10921 tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg atggggagtc tttctcgagc
    10981 aaatattttt atcgatgata caccagggat caaaatctct gaaatccgtg cgaaatgtcg
    11041 gaaactagcg caagaaaaag gcaatctagg cttgatattg atcgattatt tgcagttgat
    11101 tgaaggtaac ggtaaagaga accgtcaaca agaagtatca gatatctctc gccaactgaa
    11161 aaaactggcg aaagagctga aagtacctgt cattgctctt tcacagttat ctcgtggagt
    11221 agaacagcga caagacaaac gaccagtgct aagtgatatt cgtgaatccg gatcaattga
    11281 acaagatgcg gacatcgtag ccttcttgta tcgagatgat tattatgaaa gagaaggcgg
    11341 agatgacgat gatggaaacg agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa
    11401 gaaccgaagc ggtgcacgag gaacagttga actactgttt atcaaagaat acaataagtt
    11461 ttcgtcgctt tcgccaagag aagaatttta aagaagaggt tgtgagcctt gagcaatccc
    11521 gagagctctc gtaagaagat gcaaggagta accgcaggaa cgactagagt acttaattta
    11581 aacagatctc caaagggatt gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac
    11641 ggtgttcaat cagctgaccc ttggtattag ctcagcaaac gtcaaaatat gaggaactat
    11701 tttgacgttt gtttctctaa tactcaggtc aaaacgctga tcttacgacc tcttttcctt
    11761 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa acatgagaag
    11821 cagttttttg aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctctttc
    11881 ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga
    11941 gaagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc
    12001 tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt
    12061 tgagaagcaa ttttcgtaca ttccttctta tttcttgaag ctgaccactt ctgttacaac
    12121 ctctttttta ataaccattc taaaaagata aagttcgttt tttaagcgtt tttataaaat
    12181 aaaaataaaa atcattcgat tttatttgaa atatgtatct attattgata caatggttca
    12241 ggattaaata atatagaacg aggtgttcac atgtcatctg tagtagtagt aggtacgcaa
    12301 tggggcgatg aagggaaagg aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt
    12361 gcacggtacc aaggtggcga caatgcaggg cacacaatta agtttaacgg tgtgacttat
    12421 aaactgcatt tgattccttc tgggattttt tataaagaca agatcagtgt gatcggaaac
    12481 ggcgttgtag tcaatcctaa atcattggtt aaagaattag cttatttaca tgaaaaaggt
    12541 gtagaaacaa gtaatttgcg tatttctgac cgcgcgcacg taatcttgcc ttatcatatc
    12601 aaattggacc aattacaaga agatgctaag ggcgaaaata agattggtac aacgattaaa
    12661 ggaatcggac ctgcctatat ggataaagca gctcgtgtag gtatccgtat tgcagactta
    12721 ttagataaag aaatttttga agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa
    12781 tttgtaaaaa tgtttgattc agaaccactt gattttgacg atatttttga agaatattat
    12841 gaatacggac aacaaatcaa acaatacgtc acagatactt ccgttatctt gaatgatgct
    12901 ttggatgcag gcaaacgcgt gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac
    12961 cagggaactt atccttttgt tacttcttct aacccagtag ccggtggggt aacaatcgga
    13021 agcggtgtag ggccttcaaa aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca
    13081 cgtgtcggtg atggaccatt tccaactgaa ttatttgatg aaacaggaga acaaatccgc
    13141 gaagtaggac gagaatacgg tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat
    13201 tctgtagtta tgcgccattc taaacgagtt tcaggtatta ccaacctatc tttaaactcg
    13261 atcgatgtat taagtggatt ggaaacagta aaaatttgta cggcttatga attagatggc
    13321 gaactaatct accactatcc agcaagcttg aaagagttga accgctgcaa accaatctat
    13381 gaagaattgc caggctggtc agaagacatt acaggatgca aaacacttgc agaactgcca
    13441 gaaaacgcac gtaactatgt acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca
    13501 ttttctgtcg gacctgatag aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc
    13561 taaggataaa aacgacgact gaatcaggac gttcttacca gagcgagtcg aagtgtttat
    13621 ctatacgata ataataactt gaagaggtag tgaaagagac gtttagcctt attgccgata
    13681 ggctgtctct tgaacactgt ttattcagaa taagggactg tgacaaaaac cgtgtcacag
    13741 tccctttttc cgaa
//
LOCUS       DAYYKN010000070        13732 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000070 DAYYKN010000000
VERSION     DAYYKN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.070"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..1345)
                     /locus_tag="ACHBB7_001771"
     CDS             complement(143..1345)
                     /locus_tag="ACHBB7_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9209722.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="ACHBB7_001772"
     CDS             complement(1348..1542)
                     /locus_tag="ACHBB7_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9209723.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="ACHBB7_001773"
     CDS             complement(1589..2437)
                     /locus_tag="ACHBB7_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM9209724.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="ACHBB7_001774"
     CDS             complement(2642..2992)
                     /locus_tag="ACHBB7_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209725.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3170..4033
                     /locus_tag="ACHBB7_001775"
     CDS             3170..4033
                     /locus_tag="ACHBB7_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9209726.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="ACHBB7_001776"
     CDS             4657..5649
                     /locus_tag="ACHBB7_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9209727.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="ACHBB7_001777"
     CDS             5665..6549
                     /locus_tag="ACHBB7_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9209728.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="ACHBB7_001778"
     CDS             6598..8205
                     /locus_tag="ACHBB7_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9209729.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="ACHBB7_001779"
     CDS             8206..9690
                     /locus_tag="ACHBB7_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM9209730.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="ACHBB7_001780"
     CDS             9867..10859
                     /locus_tag="ACHBB7_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9209731.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="ACHBB7_001781"
     CDS             10911..11780
                     /locus_tag="ACHBB7_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9209732.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACHBB7_001782"
     CDS             complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACHBB7_001782"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM9209733.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4214 a         2322 c         2832 g         4364 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tt
//
LOCUS       DAYYKN010000071        13706 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000071 DAYYKN010000000
VERSION     DAYYKN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.071"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..1536
                     /locus_tag="ACHBB7_001783"
     CDS             109..1536
                     /locus_tag="ACHBB7_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9209734.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1628..1993)
                     /locus_tag="ACHBB7_001784"
     CDS             complement(1628..1993)
                     /locus_tag="ACHBB7_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM9209735.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1990..2355)
                     /locus_tag="ACHBB7_001785"
     CDS             complement(1990..2355)
                     /locus_tag="ACHBB7_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM9209736.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2547..2795)
                     /locus_tag="ACHBB7_001786"
     CDS             complement(2547..2795)
                     /locus_tag="ACHBB7_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209737.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2794..2922
                     /locus_tag="ACHBB7_001787"
     CDS             2794..2922
                     /locus_tag="ACHBB7_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM9209738.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2979..4673
                     /locus_tag="ACHBB7_001788"
     CDS             2979..4673
                     /locus_tag="ACHBB7_001788"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM9209739.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4773..5069
                     /locus_tag="ACHBB7_001789"
     CDS             4773..5069
                     /locus_tag="ACHBB7_001789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209740.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5447..6580
                     /locus_tag="ACHBB7_001790"
     CDS             5447..6580
                     /locus_tag="ACHBB7_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM9209741.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6611..6931
                     /locus_tag="ACHBB7_001791"
     CDS             6611..6931
                     /locus_tag="ACHBB7_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM9209742.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6960..7424)
                     /locus_tag="ACHBB7_001792"
     CDS             complement(6960..7424)
                     /locus_tag="ACHBB7_001792"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9209743.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7555..8808)
                     /locus_tag="ACHBB7_001793"
     CDS             complement(7555..8808)
                     /locus_tag="ACHBB7_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9209744.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8837..9154)
                     /locus_tag="ACHBB7_001794"
     CDS             complement(8837..9154)
                     /locus_tag="ACHBB7_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9209745.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9180..9500)
                     /locus_tag="ACHBB7_001795"
     CDS             complement(9180..9500)
                     /locus_tag="ACHBB7_001795"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9209746.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9964..11379)
                     /locus_tag="ACHBB7_001796"
     CDS             complement(9964..11379)
                     /locus_tag="ACHBB7_001796"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9209747.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11396..12712)
                     /locus_tag="ACHBB7_001797"
     CDS             complement(11396..12712)
                     /locus_tag="ACHBB7_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9209748.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12862..13560)
                     /locus_tag="ACHBB7_001798"
     CDS             complement(12862..13560)
                     /locus_tag="ACHBB7_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM9209749.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4351 a         2784 c         2359 g         4212 t
ORIGIN      
        1 ttaacggcat ttatatgctt tttcagaaat taactgatga tttcaaaaag aaaacatgtc
       61 ataatatttc ccatattgtg caaaaaaata caaggcggag gctctactat gattgaagaa
      121 tatattgaga aaaatatttt aagacagctg tttttatgtg ggcaattcta tgtaaataaa
      181 gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt gcaaaaccac tttactcaat
      241 gatatcaata acataaaaaa agaatttgaa gaacagatcg cttatacaca tcgggaaaag
      301 gaccgttaca ctttatattt ttcagaacat atacctcgat gcaagatcat gcagcaattg
      361 tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt tggaagaaga tgaacctgat
      421 tatcttcaat taactgaatg tgaattcatc tctgtcagca aagcttattc attaaaaaaa
      481 caagtattag cttattttaa tgactgcggg atcgaaatcg accgctactc ccctcgattc
      541 actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg cggttttaat
      601 tcttgggagc tacctgaatc gttttttgaa cgtgccgatc gtttcattga atccgtaacg
      661 gaaaatagtg gcagatttta tgataaagaa aataaagaaa tcttatcgat tggttttgct
      721 atcagtttcc tacgccagca agtgtgtgct gtgacaatag attcaaaatt tatcgaagaa
      781 atcaaaaaac gtcctattta caattatgtc gaatcagcat gggaaaacac tgatttccaa
      841 acctattata aaaaagaaga attcgccttt attttgacac tattcaatct ttgtaattat
      901 ggtttccatt cttatcagtt gattgcggaa gactttcaac agctacacca agtgtttatt
      961 gacaatactc ccgaaataaa agaactagtt gctacctttg aatctcactt caatcaagaa
     1021 ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc tgatgcgttc ggcttgggat
     1081 aactatcaat tattcatgcc agaaaaattt tatcttttaa atgaggaaca gacaaattta
     1141 tataaagaag tccagacaat tttttccagc tggtccagcc agttacctta tgatctacgg
     1201 ttgaatccta attgcatgcg ggcttttgtg atcgaattga gcggaattct ccgtctgaca
     1261 aaagaacatc tgactatcta tatcgtcaca aattcggatg ttcattatct gatttatcgg
     1321 gaagccttgg aagctgtaac tactttcgat tttcaagttg caccaacaat ttattcaagt
     1381 atatctgata taaagaagta tgcgcagcag tcctcaaacc gggtattatg tgaacgtaca
     1441 ttatatacac cagatgcggt ccaatatgaa aatattattc ctatttcgat caatacgatc
     1501 gatcgagcta tcatttcagc tgtgcaaaac aaataagggg ctgtgacaaa aatcatgtca
     1561 cagcctctta tttgttgaag atgcccattt ctgtaacaat ctcttgatac acgtcaggct
     1621 cacaacctca cttattttgg acatctacgg gtccgatctc tttatttaaa aaataagaaa
     1681 tgatggttcg gataactacc aacgccgcta gttggaaaat atcctgcaag gtaggtttga
     1741 taatcgattc gatgatccca gctgaaacaa gaatttccaa acttagaagt atatagcctc
     1801 ccaacatctt tttaatagca ttgttctcgc tgactcggtc gctttgcact tttgtatgga
     1861 caaataaata tttcaaaaaa tccttgattg atagacaaac accccaaacc aataaacaga
     1921 tagcaaagat atcaagtccc agtacaagtt tttcgaaata tggaagaaga aactgcatca
     1981 actctgtcgt catcctacct ctcctcttct ttgtttttct ttgtcttcta tttcctgatt
     2041 caagaaataa gaaatgactg ttcgaatgat aataataagt gcaagcttca aaatatctgt
     2101 ccaagtcggc ttgataactg actcgatgat atcagcaacg actagtactt ccaagcttaa
     2161 caaaatataa cttgctagca gttttcttgt atctgcatta cgagtattac gttctgcata
     2221 gtttttcttg acatgagacc aatcaaaaag tttgaataga acaagaacga tcccgacaat
     2281 taagataccg atagaacaaa tatttaaaat agcaatgatc cattctagaa attcaattaa
     2341 atttgagcga aacatatacg ctacctactt tcttttaatt tgttcctatt cttttattgt
     2401 aaaagaaagt taccttgttc gtctatttaa aagttgttct aatgataacg gttcgcttaa
     2461 atttttcttt ttcactctcc catttttctc cctccatcca taaatcgtgg aagtatccaa
     2521 aacaacgtag caagttaaaa taaggcttag tccgtcatca gcttttcaaa gaagcactgt
     2581 ctcggtgctt tttttgtacc caaatttcta aaaatagaac catacacctc ctcaaatctt
     2641 ttcttcttac ctaccctatt tttatttaca ggaaaatcag acacttcttc ttttttgtca
     2701 aaatttcctt tttcttctcg tcctatcatt tggcattcta ataaaaacga actatccttg
     2761 gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat caaagtcggc
     2821 atcggtttag gtttgcttgc ccttggcgct gcatatctag gaaaaaagac aggactattc
     2881 gaagatgaca gtcatttata tgatgaattc gaatcaatct agcatcctat ttagttgaat
     2941 cactacggac atacttggaa gacattggag gaatcacaat gagaaaaagt aaagcaataa
     3001 tgattggtgc cggtattgcc aatatggcag cggctgttta tttgattcaa gaaggcaaat
     3061 ggaaagggga tcagatcacc ttttattcat tggatgatca cggttcaaat gatggtgcac
     3121 cgaccgacac tgtagtggat gaatattgga ataaaaacca tccattagaa aaccaaaaag
     3181 gctatattgc tcgaggcggt cgcatgctta attaccgtac ctatgtagat ttgatggatc
     3241 tgttaagtcg aataccttct gttactgagc cagggatgac tgctgaagaa gatacgagag
     3301 attttgatgc aaaacaccga acctttgaca aagcacgttt gatggaaggt ggcaaaggaa
     3361 tcattgatgg cggaaaactg ggattcaata ataaagaccg tattcttctg actaaactga
     3421 ttgctatgcc tgattcagaa gaagaaaaac tagacaatat cactatcgaa gaatatttca
     3481 aagatgatcc acactttttt gaaacaaact tctggtttat gtgggaaaca acatttgctt
     3541 tcagaacacg tagttctgct caagaattgc gtcgctatat gcatcaaatg atttatgaat
     3601 tcactcaaat cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa tacgaaagta
     3661 ttatgcttcc tttgatcgag tacttgaaag aacaaggatg caaaatcatt ttgaaccgcc
     3721 gtgtcactga ctgggaattc aaagaaacac cgatgcaaga tgaaatcact gtgactggtt
     3781 tgaaaattac caatacactg actcaagaag aagaatacgt tgtggtcgat gaagatacag
     3841 cagttatttt cactaatggg tccattacag attcagctac tttaggagat tacgaaacac
     3901 cagctgtaga aaatatggat tatggtgcag cttctagtct ctggaaaaaa gccagtgaac
     3961 gtttctacaa cttgggaaat cctgacaaat tctttgctga tcgagatgca agcgaatggg
     4021 tcagctttac cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc cgtatcacga
     4081 ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac cacaccgatc acgccgttag
     4141 gccaaaaaga tgtcaatatg tctatcgtcg tgcatcatca accgcatttt acgtctcaga
     4201 aaccaaatga aacagttatt tggggttatt tcctttatcc aagacgacgt ggcgaatttg
     4261 tggataaaca gtatattaaa atgaatggaa aagaaatgct agaagaactg attggacaac
     4321 tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa attttcgaca
     4381 gcgtaataaa taatatccct gtctatatgc cttatgcttc cgcgttgttc aataatcgtg
     4441 ccaaaagcga tcgaccaaaa gtcattccta aacattcgac caatctggcc tttacaggag
     4501 aatttgtcga gcagccttat cagatgatct ttactgaaca aagtgctgta cgttctggag
     4561 aaatcgctgc tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa acaccgcgtt
     4621 acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa taatcaagag
     4681 gttgtgaaaa agctgccctg catcaagtaa taagaacaac caggctaaaa cagcttctta
     4741 tgtttttgat ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttaccaa
     4801 gaggttgtga aaaagctgcc ctgcatcaag taataagaac acccaagcta aaactgcttc
     4861 ttatgttttt gacttggttg gacttattac cgcagatgca agcttttgaa caccatttac
     4921 caagaggtta tgaaaaagct gccctgcatc aagtaataag agcagccaag ctaaaacagc
     4981 ttcttatgtt tttgacttgg ttagacttat taccgcagat gcaagctttt gaacaccgtt
     5041 taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg aagaaaaatt ttgtgaaaat
     5101 ggttcctcac gtttttgcat aaatttgact taataccgag aaactgatcc cttctgtcat
     5161 aacctctttt tatttatgtc tgtttctttt tgaaaacaac tattttttca acgtttactc
     5221 tcttgccaat ccaacatcat ctaacggata caactatttc aatgattacc attacactaa
     5281 aaaatcggaa acacacctct ccgaacacaa atacataaga ctatagaatg tttcatttaa
     5341 gaaaaaaagc gtttcattat caaaaaggta agatctcctt taaaatgaaa ttatctgtag
     5401 tactaataat gcagacaatt ttatgaatct gaaaggagca tctccaatga aagcagtcac
     5461 ttggcaagga aaacagaaaa tggaaatcag aaacgtagat gatccaaaaa tattagaacc
     5521 aacagatgca atcatccgta tcacagcaac agccatttgc ggctccgatc tgcatcttta
     5581 ccatcatggt gaagcagttt tggaaaaagg gtatgttgta ggacacgaac caatgggaat
     5641 cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa gggcaacgtg tggttatccc
     5701 ttttaatatt agttgtggtc attgtcattt ctgcctgaat cacatggaaa gccaatgtga
     5761 tgaatcaaat ccagatcagt tatatggcgg gcttttcgga tttggcaagt tgaacggtaa
     5821 tcattggggt ggtcaagcgg aatatctaag agttccttat gcagactcta cttcatttgt
     5881 tgtaccagat aatgatcttc ctgatgaaaa agttttattt ctatcagata ttttgcccac
     5941 agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc gatacagttg tgattctagg
     6001 ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt atggctggag cagagcgcgt
     6061 aatcgcagta gatccagtac agcaccgtct agataaagcg gctaattaca atggggttga
     6121 aacttatttc ttagaagaca cggcacaagc tggaacagaa ttgtatgaac tggctaaagg
     6181 tggcgcagat gtcattatcg attgtgtagg aatggacgga ttagagcctg tcaaagaaaa
     6241 agcgaaaaat ttagtaagct tacaaagcgg cacaatctct ccattacaga tggcatcgca
     6301 agctgtcaaa aaatttggaa cagtccaagt aactggtgtt tatatgactc cagcttcttc
     6361 ttacccgctg caagaatttt ttatgcggga tatcgctgtc aagcatggac aggcaccaat
     6421 tatccatcta atgccaaaaa tttacgcaat gattgcagaa ggactattcg atcctacaca
     6481 aatcattact cattcgctgc cactatcaga agcagcacaa gcatatgaga tctttgacaa
     6541 aaaagaagat aaaaatataa aagttgtttt gaaaccttga attcagtaaa aaatacaggt
     6601 gattttcttt atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga
     6661 agatatcgaa atacgaggac gtcgcttccg cttctatttc caaaatcgtt acacattgat
     6721 ctcactggct gtcgatttat taaccgggat tttctatatt tttggaagcc ttgcttcctt
     6781 aactaaaatc cccgatcgat atggcaccta tttttatttg gctggtgcta ttttcttgac
     6841 gattcgtcct attctcagaa tcgtaaggaa catttttatt tacgacgaaa aaaaagcaat
     6901 gaagagaaca agcaaaaaaa aagaggcatg agaatttttc aaatctcatg cctctttttt
     6961 caccctatcg tatcactttg atacagacga gcggtcgtcc cactttcttc cttgaccatt
     7021 gtcaaaaatg tccactcgta tcgttcataa aacgtggtat gatctgtaat taagtaaagt
     7081 ttttcgtacc caagttgatg catttctttt ctgatttccg ctaatatccg cttggctatc
     7141 ccctgcttac gcttttccgg ttcaacaaac agggcacaaa gatttggtgt cagatcaggg
     7201 cggttatgga agtcgttttc aatgacgccc gctcctccga cgatggtttc tttttcatct
     7261 aagaccacat accattgagg aatactttcc ttattagcga ttccagtacg aatactttcc
     7321 tcatacacaa cagcaggaac tccccatttt gatgaaaacc aattcgcgct ttgttttact
     7381 aattctgggt gttctcttaa tttgatccag ttatatgtaa ccaatgttta aacctccgac
     7441 taaaataaaa cgtttacaaa aattatacaa gtctttatga aaattagcaa tgacaacaat
     7501 agtgatgtaa aaaaaaaacg gaaaacaatc acttgttctc cgttttttga ttaatcaagc
     7561 atctaaagct tcttttcttc tttcttggat cgtagctacc ttgacgaacg gtagatagat
     7621 caaaatagaa agaaccaaat tgacagcggc tagtacacca cctgcaatac tttttgttgc
     7681 caatacaccg ccaataattg gtggagttac ccatggaggc ataacggtag ctgctggcac
     7741 taaacctgta gaagtcgcaa agtaagcaac actaactaag accattggtg tcaaaataaa
     7801 tggaatgaac atgatcgggt tcaacacgat aggtaaccca aacatcgttg gttcattaat
     7861 attgaagaca cctggcgcaa tcgacaagtt tgttacaacc atatatggtt tgtttttacg
     7921 tcctactaaa taaatagcta acagtaaacc taaagttgca cctgttcctc ctaagttgac
     7981 aaagctgtca aagaaaggtt tgttcacgat aaacggtgcg ctatgcccag ctgcgattgc
     8041 attaacgttt gcttcaatgg ctggcgcatt gattgtttgc atcaaaggat cgaccatatt
     8101 cgctccgtgt aaaccaaaga accataagaa tggtgtgata aatgcaagca gtaaggcaga
     8161 tggataagag tttgcaagtc ccataaatgg ttcttggacc actgtataga aagaacctac
     8221 gatatcatca acaccgaatg cagcaaaaat tgcagcaact aaagcaaacg cagagatagt
     8281 gatcattgct ggtagtaatg aagcaaatgc ttttgcaact gccggtggca caccatcagg
     8341 cattttgatg atcaattttt catttccgct taaacgtaca aagatttctg ttgaaataat
     8401 cccaacaata atagcaataa acaaaccgtt tgagctcata cctaatgctc cgcctaaagc
     8461 gaagaaagag cctaaagaaa tcacacctgc ggaaacaccg tcttttcggt agccgttagc
     8521 taaatgataa gcaagcaaga aagcaatcaa gacagaaaga atggcaaatg taccgttcca
     8581 agcagcagcc ccgaatcctt tccatgattc accaccaaaa attgagttca tcaattcttg
     8641 gaacccggga atcggtaagt tattgatcat tactgctaat gctcctaaga tcattaacgg
     8701 catactcacc ataaatgagt cccgaatcgc aactaaatgc cgttgtgcac caatttttga
     8761 tgctacaggt ataaaatgtt tctccataaa tgcaacaaat ttatccataa atcttccctc
     8821 ttttctttac tgtagattac gcgttcaatc gacgatacag atcaatgatc tcttttgcta
     8881 aatctgtaaa agctatagat gtcatcagat gatcttgact gtggaccgtc aaaagcgtga
     8941 cttcaacgtg atttccttgt gcttcttgtg tcagcatctc tgtttgagag tgatgtgctt
     9001 ggactaaaga ttcctctgca tctttgattt tttcatctgc taaagtgaaa tcaccttttt
     9061 tcgcagcagc aatcgcttcc attgcatcgc tttttgcatt tccgccaaac atgatcagac
     9121 ccatgatggc ttctagattt ttttgatctt ccatttattg tatcctccga taatttttat
     9181 tatttgttta ttagtttttc tgcttggtcg agtacttttt caccattcat catgccatag
     9241 tcagccatat tgattacatc taaaggaatg cctttaggtg ctagcttttc agcaaattga
     9301 ttttgcataa agcgtacttg cggtcctaaa agtaaaacgt ctacattttt agattctagt
     9361 tgattatccg catctgaagc agatacagca aagatgtctg cttccattcc ttttgcttct
     9421 gctgcctttt gcatttttgt gactaggaga cttgtactca ttccagctga acatacaagc
     9481 atgattgttt tcttttccat tttaaacacg ttcctttcta tacttacact tgtatataag
     9541 caagtagcgt gccaacttta aaagcgcttt aaaaagttga tatcatcaca tttaaggtct
     9601 ataccaagtt gcatacacac taaaaaagaa agcgcacact aaacacacaa attaattttt
     9661 attacctttt ttcttttaac gtgcacaaaa aaagttgtga cacaaaactt gtcacaactt
     9721 ttttcaaata aacggtattc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa
     9781 aacataagat gttgttttta ctttgctatt cttattactt gatgcaggac agctttttca
     9841 caacctctca ataaacggtg ttcaatcagc tgaccctggg tattagctca aaacgctgga
     9901 ttcacaacct cttttatttc aaataaccag taaatacaat gaacatctac aaaaaacttc
     9961 tatttattta tagatttcgt caggcaaatc caattctctc ttatcaatca aagtctgata
    10021 ccacgaagca gattttttca atgttctgct tcgttcatcg ttcaattcga ttcgaactaa
    10081 tccatatcga ttcttaaatg cattcattgg tgacacacag tcagtgaatg cccataacat
    10141 atagccttcg caatttgcgc cttcttctgt tgctttgatc aagtaatgca aatgctgtcc
    10201 gataaattcg atacggtaat catcttgcac gattcctttc tcatccatat actgttcttc
    10261 atattctctt cccataccgt tttctgttat cagccaaggg atattatgat aattttcttt
    10321 tagatacatg gctatatcat acatgatttt cggatagatt tcccatcccc tggaattatt
    10381 cattttcttc ccaggtaaat caaagacttc ataatatcgt tcaggatgga aaggtgtctc
    10441 ttcattccat tgataacgag gggcgcgaac gcgtttagga taatattgat tgattccaag
    10501 gaaatctact gtatgatgct gaatcgtttc taaatcctct gttgcaggat cgaaataaat
    10561 cgattgtttt tgacacaact ccaggaactc ttctgtgtag tatcctttta ccatcggatc
    10621 aaaaaatact cgattataaa acaaatcata catataagca gctttttggt cttctggaga
    10681 attggatcga gcatagacca tttcaggatt taaaatacag ccaatccttc caccataatt
    10741 tccgctgcgg taaatctcta ctgctttagc cgttgctaaa actttatgat aattccacat
    10801 catccacttt tttgtgtttt gttcatgcgg ccagcgaaca gcatccaaat agcaacgcgt
    10861 ttggggaacg attggttcgt tgaatgtaaa ccagtttttt accctatcac cgtaacgatc
    10921 aaaggcaatt tgagcatact tagcataaaa atcaactact tttcttgaag accagccatc
    10981 atatttttcc aaaagaaaag ctgggacttc ataatgttcc aaacaaatca tcggttctac
    11041 gtctgcttcg atcaaagagt cgatcatttc acttatatgc tgagcatatt cttcatctac
    11101 tatacagttc tcataatcag taaaaaaacg agcccaattg atagaagtcc gataatgtgt
    11161 cagatggatg gatttcatca gatccacatc ctgctggtat tgttccataa aattagttgc
    11221 tacggctggg ccatacccat tatgccaaac aaaatgttca tttttatacc agctatctat
    11281 aaacgaatcc tgtccttctt tttttccttt ccaaccttcc gtttgccaag cagaagatgc
    11341 agcaccaaga ataaaatttt ttggaatagt aattttcatg atttcctcct gttttttaga
    11401 tcccatcatt ttccacacga tttgctactt ttacaaatgg cagatagatc aaaatagaaa
    11461 caataataca aacaagttgc gtaataactg caccgatatt tcctgctgtt gccagataag
    11521 cattgacgat cggcggcatt ggccaaggga ccattacaac tgctttcgcc gcaaatccta
    11581 aagcggttgc aaaataacca atcgttccag tgataagagg cgttaagata aacggaatag
    11641 ctaaaattgg attcagcatg atcggcatac caaaaataac tggttcattg atgttgaaga
    11701 tcccaggagc aagggataac ttggtgattt gtttcatatc ttcccgtttt ccaactaaca
    11761 atactgcaat cagtaaaccg atcgtgactc cagacccgcc catactcata tacatatccc
    11821 aaaatggcat agtaataatg ttcggtactt cttttccagc ttcaaaagct tccgtattta
    11881 cagcaattgc cgctaaaagc aacggttccc gtatcggctt caccatttga tttccatgga
    11941 taccaatcac ccaaaagact tgcgcaataa acatcaatag caaaataccc ggtaaccctt
    12001 gtacgacacc ttctaatggg cgttgtacga cattgtaaat gatatcataa aggtacattc
    12061 ctgtaagttc tttgatgata aatcccgcag ccgcgatacc cgatacagtc agaatcgtcg
    12121 ggaataaagc agaaaaacta gcagaaacat tcgttggcac ggtatcaggc attctcagct
    12181 gtagtttctt ctgtttcctc aaccagcaat aaagttcaat agaaaggatg gagaccaaca
    12241 ttcccaaaaa taaccctttc gtatctgtat attgtttagc caatacgttt tctactataa
    12301 cagtctgatt ttcattcact aattcataaa cagttggatt aacagataaa taagacatca
    12361 ctgcgatgat accagggaat acaccatcca ctttattcag cttggctaat tcgatcccta
    12421 tcagaaaaac agcatagatg gtcaagaaac ttagtgtgac atagctgatg gcagatgcga
    12481 taggctttaa ttcttctaaa aaacgcaaga aacttatctg tgctaagcca ttttccgagt
    12541 caaagaccat tgctgaaaac aatgtgccaa aagcacccgt aataattacc ggaatcaatg
    12601 cagcaaatgc gttttttatc accatgatat accttaaatt attgattctc gttgccacgt
    12661 tccctaaaac attggctaat ttatccataa aactcattcc agatcccctc attcctgctt
    12721 cttctccttg acgtcatctc tttttgtcta ttagtcagcg cttacatttc gatgaacaga
    12781 aactatttac tcctcttttt taccaaatat aaaacaaaaa ttcatctgat gtatttatca
    12841 taaaatacat cagatgaact gtcaattagt ttctttttct cctattattt tctgtaattc
    12901 tctccgattt attgccatat ggacagtcat tgcatcaaac gcagcaagtg cattccgcct
    12961 ttcgatggcc tgaacgatct ctgtatgaat atcgatcgtc tcgattttca gatgctggat
    13021 atcataattt ttattaaaca aagcaattga ttcattaatg attgggataa tatgcagcaa
    13081 ggctacgttt ccgctagctt ctgcaatcat cgtatgaaaa tcgatgtcta attgaacatg
    13141 ttcttgtgtg ccttctcgaa aagaagtttc aatcttttgc gttaattttt tcatttggac
    13201 gatttgattc ttcgttgcat attccgctgc caaacctgcc atcttaggct ccaataaata
    13261 tctcagttca aataagtctc gtatcatccg atcctgatct tcaatcaaag aaaaaccgag
    13321 tgggtcattg gcaatccctc gcttatcact gacaaatgtc cccgctcctt gccgaacttc
    13381 aaggatattc ctggaagcta gagcccgcac agcttctcgc accgtgcttc gccccacctc
    13441 taaataatcg gacaattcat attcattcgg cagtttatct cctacttgca actgcatttc
    13501 ctttaaatat ctaaggatct tgtcagtagt tacttctact agggttttat tttcttccaa
    13561 accacatacc tccttttcat ctgatgtatt ttttcgtaat tattataaca cgtttgaatc
    13621 atctgatgat tttttgtata caaaattgat taatccgttc atagacatac aaacgaagaa
    13681 gatatttacc ataaaaaaga ctagga
//
LOCUS       DAYYKN010000072        13647 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000072 DAYYKN010000000
VERSION     DAYYKN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.072"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..602
                     /locus_tag="ACHBB7_001799"
     CDS             <1..602
                     /locus_tag="ACHBB7_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209750.1"
                     /translation="SGKVFNSNGNVYYNYFYFVFWVAATLLGYDKIQIAGIPIHMQYK
                     LVLSGIFSEVLPDIYDDHYDSDGTCKVSIEKENFDDIDGYDSVNLLIIDTYDIKMSEL
                     SMENQTYPTIIVRGNSIDGVRKVNRSLILEIKKTMDEIQKSDFKKVFVASTSNPKNSI
                     NIINSSFRFFGRSRRFKLYVLQKDYASNGKYSKKYRIFI"
     gene            640..1029
                     /locus_tag="ACHBB7_001800"
     CDS             640..1029
                     /locus_tag="ACHBB7_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9209751.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1016..3463
                     /locus_tag="ACHBB7_001801"
     CDS             1016..3463
                     /locus_tag="ACHBB7_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9209752.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            3468..5576
                     /locus_tag="ACHBB7_001802"
     CDS             3468..5576
                     /locus_tag="ACHBB7_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9209753.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            5573..6577
                     /locus_tag="ACHBB7_001803"
     CDS             5573..6577
                     /locus_tag="ACHBB7_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM9209754.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            6596..7507
                     /locus_tag="ACHBB7_001804"
     CDS             6596..7507
                     /locus_tag="ACHBB7_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9209755.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            7694..7981
                     /locus_tag="ACHBB7_001805"
                     /pseudo
     CDS             7694..7981
                     /locus_tag="ACHBB7_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8169..8384)
                     /locus_tag="ACHBB7_001806"
     CDS             complement(8169..8384)
                     /locus_tag="ACHBB7_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209756.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8643..8852)
                     /locus_tag="ACHBB7_001807"
                     /pseudo
     CDS             complement(8643..8852)
                     /locus_tag="ACHBB7_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(9155..10594)
                     /locus_tag="ACHBB7_001808"
                     /pseudo
     CDS             complement(9155..10594)
                     /locus_tag="ACHBB7_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(11142..11813)
                     /locus_tag="ACHBB7_001809"
     CDS             complement(11142..11813)
                     /locus_tag="ACHBB7_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM9209757.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(12162..13613)
                     /locus_tag="ACHBB7_001810"
     CDS             complement(12162..13613)
                     /locus_tag="ACHBB7_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9209758.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT         4342 a         2479 c         2599 g         4227 t
ORIGIN      
        1 attcaggaaa ggtgtttaac tctaatggaa atgtgtacta caactatttt tattttgtat
       61 tttgggtagc agcaacttta cttggttatg ataaaattca gattgcagga attcctatac
      121 acatgcaata taagttggtt ttgagtggaa ttttttctga ggtgttgcca gatatatacg
      181 atgatcatta cgacagtgat ggaacatgta aagtaagcat tgaaaaagaa aatttcgatg
      241 acatcgatgg atatgactct gtgaacttac tgattattga tacttacgat ataaaaatga
      301 gtgaattatc tatggaaaat caaacatatc ctacgataat tgttagaggg aattcaatag
      361 atggagttcg taaagttaat agatctttaa tcttggaaat aaaaaaaaca atggatgaaa
      421 ttcaaaaaag tgactttaaa aaagtatttg ttgcttcaac atctaatcca aaaaatagta
      481 tcaatattat taattcatcg tttagatttt tcggtcgatc acgtaggttt aaactttatg
      541 ttcttcaaaa ggattatgca agcaatggaa agtatagtaa gaaatataga atttttattt
      601 aagaaggaat ttttccttct ttttattttg gaggaaacta tgaaaaaaat aaaaagctac
      661 accagtattt gggcagttga aaaagtcatt tatgcgatta atgactttca gctgcctttt
      721 cctgtgacct ttaaccagat ggcttggttt gttttatcct tgatgtttgt cattgtattt
      781 gcccatgtgc ctccgctatc catgatagag ggggcttttt taaaatactt gggcattccg
      841 atagcggtca cttggttcat gtcgcaaaag acctttgacg gtaagaaacc attgggattt
      901 ttacgatcct ttatcagcta tcacttacgg tttaaggtca cttttgcagg aaagaaagtt
      961 aaagaacaga agaaacgatg ggacgaacca atcaccttgg taaggagtgt gaattatgta
     1021 cccgattaag tatattgaaa ataatcttgt ctttaaccaa gaaggcgagt gttttgctta
     1081 ctatgaatta gtgccttaca attactcctt tttgtcacct gaacagaagt atcaagtaca
     1141 tgataacttt cgccaattaa ttgcgcaaaa tcgtgaaggt aagatccatg ccttacaaat
     1201 tgccacagaa agtagtattc gtgccacaca agaacgttcg aaaaaggaaa ttaccggtag
     1261 actcaaagaa gtagcaaagc aacgaattga tttacagaca gatgcattag tatccatgat
     1321 tggcgatagt cagattgatt atcgcttctt tattggcttt aaactgattt cgacagatga
     1381 agaggtcaat ctaaagaatc tcaggaaatc tttcttttct ggacttcaag aatttgtcta
     1441 tggagtgaat catcatttga tgggtgattt tgtttcctta tccaatgaag aaattcgtcg
     1501 ctataccaag cttgaaaaac taatggaaag taaattggct cgaagattta aagtcaaacg
     1561 agtaacgccg agtgacttga cgtatttaat tgaacatatc tatggtgaaa aaggcacacc
     1621 gtttgaagaa tatgaattcc agctaccaaa gaaaaagctg aaatcagaaa cgctggtgaa
     1681 acgttacgac ttattacgtc ccactcgttg tttgattgaa gaaaagcctc ggtacttacg
     1741 gatggaacat gaaaatcatg agtcttatgt agcgtactta acaattaata ccattgtagg
     1801 ggaaatggag tttccttcct cggaactttt ctattaccag caacaacaat ttacgtttcc
     1861 gattgatact tcaatgaatg tggaaattgt gacgaataaa aaggccctag ctaccgttcg
     1921 taataagaaa aaggaattaa aagatttaga caaccatgcc tatcaatctg ataatgaaac
     1981 gaactccaat gtattagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca
     2041 gtcgaaagaa tccatgtata aactaagcta tgtggttcga gtgagtgcta aatcagtgga
     2101 tgaattaaaa cgccgctgtg atgaagtgct cgatttttat gacgatacca atgtaaaact
     2161 agtccggccc tttggcgata tgatgggtct acacaaagaa ttcttaccct caagtaagcg
     2221 atacatgaat gactatattc aatatgttac gtctgacttt ttggcgggac ttggttttgg
     2281 ggctactcaa atgctaggag aattagaagg catttacttt ggctataacg tagatacggg
     2341 gcgcaatgtt tacctgaaac cggcattggc atcacaaggg gtcaaaggct cagtaaccaa
     2401 tgctttggca tctgctttcc ttggttcgtt aggtgggggg aaatctttta gcaacaatct
     2461 cttagtctat tatgccgtcc tttttggtgg gcaagctgtg attgtcgatc caaaaggaga
     2521 aagaggcggt tggaaagaaa cgttgccaga aattgcggat gaaattaata ttcttaactt
     2581 gaccagtaag ccagaaaatc aaggcttact tgatccctat gtgattatga agaaaaagaa
     2641 agactcagaa agtttggcga ttgatatcct gaccttttta acagggattt ccagtcggga
     2701 tggggagaag ttccctgtgt tacgcaaagc cattcgaacg gttacccaaa gtgatcagcg
     2761 aggattatta caagtaatcg ttgaattgcg taaagaggac tcaccagtcg cggaaaatat
     2821 cgcggatcac atcgagtcct ttaccgatta tgattttgcc catttgcttt tctcagatgg
     2881 ttcgattacc cagtcgatta gtttagataa gcagttaaat atcattcaag tagcggattt
     2941 ggttttacca gatgcagaaa ccacttttga agaatatacc accatggaat tgctgagtgt
     3001 ttccatgcta attgttattt ccacttttgc cttggacttt atccatagca accgagaaat
     3061 tttcaagatt gtggacctgg atgaggcttg gagttttctt caagtagctc aaggaaaaac
     3121 gttatcgaat aaattagtgc gtgctggtcg ggccatgaat gccggtgttt acttcgtcac
     3181 gcaaaacagt gatgacttgt tggatgaacg tttgaagaat aatattgggc taaagtttgc
     3241 atttcgcagt accgatattc atgaaatcaa gaaaaccttg gaattctttg gattagataa
     3301 agaagatgaa agtaatcaaa aatgcatgcg agatttggaa aatgggcaat gcttgatgca
     3361 ggatttgtat ggtcgagtag gtgtggtcca agttcatcca gtctttgaag aacttttcca
     3421 tgcttttgat acccgtccgc cagttcaaga gagaatggag tgaggagatg aaaaagaaac
     3481 tttttcgtat ctttggaatt gttttaatca gtttggtcac tctgataata cttttgagct
     3541 tagttggaac ggtagctgag gcaacaggtc tggttgatga tacagttgga gccggaaatc
     3601 tttattccca atattcactt ggtaactatc agttggattt ctttgtggat agttcgtggg
     3661 attggctgcc ttggaattgg ggtgatggcc tagggaagag cgtaatgtat ggactttatg
     3721 ccattactaa ttttatttgg acagttagtc tctacttatc aaatgccacg ggctacgtgg
     3781 ttcaagaagc ctataagctg gatttcattt cagatacggc tgaaagtatt gggaagaata
     3841 tccaaacctt agccggcatt accgaaaatg gactccagac ctcgggcttt tactttgggt
     3901 ttctattatt gatgatttta gctctaggag tttatgtggc gtatacagga ttattaaaac
     3961 gagaaactac caaagcagtc cgggctgtca ttaattttgt gatgattttt ctactttcgg
     4021 gttcgttcat tgcgtatgcg cccacctata tcacaaaaat caatgatttt agctcagatg
     4081 tcagcgaagc ggctcttagc cttggaaccg agattgtcgt gccgaattca gaaagccaag
     4141 gaaaggacag tgtggattta atccgagata gcttgttttc cattcaagtc caacaaccgt
     4201 ggttactctt gcaatttgat gattccaata tagaagaagt tggcgaagat cgtgttaaca
     4261 aaatcctgtc tgtaagtcca gatgaaaata aaggcaagga tcgggaagaa gcggtcaaag
     4321 cggagattga agataatgac aatgcgaatc tcagtattac caagaccatg aaccgcttag
     4381 ggaccgtggt ctttttggtg ttgttcaata ttggcatatc cttctttgtc tttctcctaa
     4441 cagggattat gttgttctcg caaattctct ttattatctt tgcgatgttt ttgccgatta
     4501 gtttcttatt gtccatgttg ccgacttatg agagtttggg caagaaggcg attattcgct
     4561 tgttcaatac gattatgatg cgagcaggag ttactttggt gattacgact gcatttagca
     4621 tctccacgat gtttttcaat atttcggcaa cctatccatt tttcatggtt gcttttctgc
     4681 aaattgtgac ctttgccggt atctatttca aattgggcga cattatgaat atgttcaatc
     4741 tccagagtaa tgatagccaa tcaatgggaa gacgggtcat gcgaaaaccg caaatgttaa
     4801 tgaatcgaac attgcggcaa ctcaatcgaa atgtgggacg gactttggct tttggtggtg
     4861 gtgcagcagt tggaaataag ttggcaaagg aacaatcaaa atccaaattt aagccatccg
     4921 gttcttctct acgaaagaat tcacgattac cgaacgatca cgagatacca tctgacttga
     4981 ataaggaaaa gccaattcca aataataaga aacaatcccg aatgaatttg gccgggagaa
     5041 aagtcggcaa ggttctggat acccaagctt tggtgaaaga taagaccaaa caagtaaagg
     5101 atggagtacg gaatacgcca accaacttga aatacaactt ccataaagga attgaaaaaa
     5161 cgaaaaaagc accggaagag tttaagcgag gactggtcca agaaaaagca aatcgaggag
     5221 agttgcgaga aaaacaacaa caacgtagag acgaaaaaat agctgaaaag cgcaaagtat
     5281 tgaacgaaat agggaatcct catgggaaaa gaagggaaaa tgttccgata aaagcaaagg
     5341 tccaatctca aaaggataga gcaccaaagc ggattgtgag agcaaattcg aatcccgaaa
     5401 tcaaacgaaa attcgctagc caagaaatca tttcaaaagg aataaaccaa tcctcaagta
     5461 agaaaaattc ttttcaacaa gtgaaacggc ggtctactct gtccaaacga accaatcgaa
     5521 aagtacaaaa aagtttgaac cctcatccca aaccaaggtc aggtgagaaa aaatgaacgt
     5581 aaagaaatta agtgccattt cgttaacttt cctaacaatc tcttttatgg gtatcttgat
     5641 gtgtgtgggc ttattgtttg gtgaagacag tgaaggaagt agtagttcag gtgctcctcc
     5701 tggggggagt agtgtctcag aagaggtttt gaaacaccgt gcaatggtgg aaaaatatgc
     5761 caaagaatcg gatataacag agtatgttcc gattctttta gcgattattc aagtggaatc
     5821 tggtggcaca atggaagatg tgatgcaaag ttctgagtca gccggtttac caccgaattc
     5881 attaagtacc gaggcatcca tcaaacaagg atgtctctat tttgcgtctt tggttaagcg
     5941 ttccaaagaa ttaggttgtg atcaagatag tatcattcag gcctataact ttggcggtgg
     6001 ttatttggac tatgtggcaa aaaacgggaa gaagcatagt tttgctttag ctgaatcttt
     6061 ttcaaaggaa aaatcttgcg gtcaaaaaat cgattaccct aatcccatcg cgaataagga
     6121 aaatggtgga tggcgctata actatggcaa tatgttttat tgcttattgg tgaagcaata
     6181 cctaactaca acacagtttg acgataaaac agtccaaggg atttttgacg aggcgtttaa
     6241 gtatgaagga acggcttatg tatttggtgg gtcaagaccc gaaacaggat ttgattgtag
     6301 tggcttgact caatggtgtt atgccaaagt tggtctgaaa cttcctcggg tggctcaaga
     6361 tcagtatgat gccatgaccc atattgacct aaaagatgcc aaaccagggg atttggtttt
     6421 ctttcattcg acttatgatg ccggtactta tgtgacccat gtcggtattt atgcagggga
     6481 gaatcgcatg tttcatgcag gagatccagt aggctggacg aatctaacag aaagctattg
     6541 gcagcagcat cttatcggtg ctggacggta caaataagag aaaagaggaa aaagaatgaa
     6601 gataaaaatt gaacggaatc aaaaggaaaa gagcctaaaa aagaaaaaag aacgagtcgt
     6661 atccgtgggt aagcatcgaa aaatggtgct tgccctttgg cttttattat cttgtagtct
     6721 tacttttggc gtttataaaa atttcaccgc cattgatcaa catacaactc atgaaaaagt
     6781 ggttattaaa gagaaagtag tgaatacttc tggtattgaa aactttacga aggatttttc
     6841 gaaagaatat ttttcgtgga aaaataacaa agaagcaatt gaaaagagaa tgaacaacct
     6901 cgaacagtac cttacagaag aaggtttcgc tttaagtcaa gatatggttc gagcagatat
     6961 tcccactaat tccgaagttc agtcagtaaa agtcttagat gttgaaaaga gctcggagga
     7021 atttgtcgtt tcatttttag tggagcaaaa gattacagag ggaaaaaagg cacaatcgat
     7081 ttcttctgca taccgagtga cgatctttga agatgaaaac aggaattata tagtaacgag
     7141 tttgccaact atgattgcaa aacctgatag ggcaaaatac aagacaaaac aagtagaaaa
     7201 tgattcgaaa attgatgcca aaactacaga agaaatcgct gagtttttgg aaacgttctt
     7261 taaattatat ccatcagctt ctgaaaaaga attggagtat tatgttgaaa atgatgcgat
     7321 gcgaccaatc aatattgact taaagttttc gaagttagag gatccagttt acctacaaaa
     7381 cggaaaaaaa attctagtga gtacaaagat aaaatacctt gataatgtat caaaaaccac
     7441 tagctacttt cagtacaaat taatattaca aaaaaatgga aactggaaaa tcattgatgt
     7501 taaataataa tgactcaatc taaatttatg agaagttata agtagtttaa cttatttcag
     7561 gcagaagtcg cccatcctct ataggtgggt gatgaatgcc gttcggtaac tcgaaaaccg
     7621 acaattaaga catagtgcct ttttatttta atccatgtca taatcagtct atataagaaa
     7681 tggttgatat accatggaat tagatacaaa taatcattca ttatttcttc tttgttacca
     7741 ccttatttta gtagtgaaac attgtaaaca tctcattgat gatgtaatgt tagcttatgc
     7801 aaaaaactat ctttgaaaga atatcagaat cacatcacat taccttagtt gagtgaaatc
     7861 gtgataaaga ctactttcat attatgctca aagcacaacc taaaaccgaa ttaacaaaat
     7921 tcagcaatgc ctaagctcat gtctgataaa atgagacttt ctaagggtca aaaatttcct
     7981 ttgaatacga tcattagctt agataaaagt aaaaacctta gaactacggg gatagcttgg
     8041 ttaataagag atatcgctgt taataaagac ataagcttgc aagtatgctc tattcccaag
     8101 aatctcccac ttcaagcact aaggatcgtt aaattcagct aagtggcaag tagttcacta
     8161 gacgataatt atctccatat ttcaatctca gcttttccat ttttcatagc aaattccgtt
     8221 atttttatag catctaaaag attcagcttt tccactgatc tttcaccact tttcagcttt
     8281 tttattgtgc ttaaattgat accagtacct tttgatattg aattggaagt aaaatcatta
     8341 agggtttttt ttattaaatt catatccgca attccagcgt tcataagaat gaagattctc
     8401 ctctcataga ctactttgaa aatggtacta cagtggctgt ttatatattt cagtaggtta
     8461 ctgtagtggt cattttatta taattagaaa aggaacctaa atcaaaaatg ctaaagaaaa
     8521 ttttcatagt attaaaccta tcatgactaa agccacggaa ttcttgataa aagagtatac
     8581 ttgcaagcct atgtctttac taacagcgat atctcttatt aaccaatcta tccccgttgt
     8641 tcctaaggtt cttgatgttt tctaaggcaa tgcttgtata catagccctt gatcaaaata
     8701 ttgatactaa cgttgacgtt tcgatcatga tgtgtacggc aaataggaca agtccattct
     8761 cgaattgtga atgacttctt accatctgtg tgtccacatt ctgaaccaat ttgactagac
     8821 ggaaatcatt tatctatctt gatgattttt ttgacagaag tatcgaaaat ctttacataa
     8881 tctgggtgtt gcattcttat gcagatgaga gcgttagctc cgtattcata accagttgcc
     8941 tctaaaacgt agtccatcgg tggataccta gcttgcggtt gccagtggct ttcgcagtct
     9001 tcttgtaatc aaatcgagca tctaattgag tactagtatt ttgaagataa tacttgagtt
     9061 tttcggtttt cagaagttca cttcatcttc catcatccat ggaaatccca ctcacccata
     9121 cgcaggcctc caaaaattct tcaacaatgg tttttcagac atttttctga atacctcctt
     9181 gttttttgat acgccagata gtcaggaaaa aggcaacaaa acacaaaaca aagctgccta
     9241 aaaaggtaat cttcattcca tataaaaaga catccggccg atctgcgata tagttagtta
     9301 ctcgttggcc catcttagca gacatcgctt gatataagac cgtcgtagaa agcgaaatcc
     9361 cgatgaccat ccccaaattt cgggcaaagg aattcatgga gcctgccagt cccaaatttt
     9421 cgatagtgac agaactcata accatcgtat tatttgggga ctggaacaag gcattgccgg
     9481 cacccatgat ggcagtagcg acaatgtaca gccacaaagg agagtccact gacaaaaaca
     9541 tatacatgac ttgggttacc gacaataaac ccaagcctac taatacaaaa atctccgggc
     9601 caacccggtc ggtcaggtat ccgcttaaag gcgccccgac gaccatcatc aagggaaaga
     9661 ccatcatcaa cagtccggca tagctaggac tcaacccgcg agcattttgc agataaaaag
     9721 gaatcactac attaacaaag aagttggaag aaaaaatcaa caaggctgta atcaagctca
     9781 tggtaaaaat cttgttctta aaaataccga aagaaatcaa tggagaggct gctttctttt
     9841 caacttttaa aaagctgaca aaggcaatca ctgaaattac gaacagtaca agggggactg
     9901 gatggaaaaa gccactttct tgccccaaaa agattgttcc aaaaaggctg ataatcgtag
     9961 ccgcaatggc agcaaagccc cctccatcga ttgtatggcc gctcatggtg atatcttttg
    10021 gtaagaattt ttcagcaatc aacatcgtta cgattcccac cggtacattg atccaaaaaa
    10081 tgtacgccca agaaaattgg gacaaaatca agccgccaag acctggtcca gcaatcgagc
    10141 ctaatgaaac aagcgcaccg attgctccca atgcccgacc acgttcatgt atgggaaaga
    10201 cttccgtgat gattccggca tttgtagcca tagtcatact agcaccaatg gcttgtacca
    10261 cccgtgcgaa caattaaata cctagcgaat gattgaaccc acacaacagc gagcccaaga
    10321 caaagatcag cgtaccaatc cgaaatactt tgattttccc acagctatcg ccaattttac
    10381 caaacagcaa caaacaagaa cagacaatca tcagataaac ggacactacc cattccgctt
    10441 gattcattgg tatgcccata tcatctgaga tggtaggtag tgcaatattg acaatactgc
    10501 catccaatgt ggacatgaag gtaaacatag atatagcaat caaaatccac cagcgatttt
    10561 tttggacttt tgcatcttct tgataactat gcataatagg atttcctttc taatgatcgt
    10621 gtagctaaga acaatataac ctatgtctac tgcccaacaa cttctcgcga ctctagccat
    10681 aggaagcttg agtaatttcg atttgttatt ctcatgtaac catgtaagtt tgagatgctt
    10741 tatttgaact tactctatag taactagtga taattcgctt tactttcttc tagcaatttc
    10801 taaaagtcga cttcattgtt cttcagatta atagtgtgag ctatctcgtg agacgtaaga
    10861 aaatttccat tctacttttt cccaattttt tcatatttat ggtgttgcac ctatttgttt
    10921 tccaatttgc ttttcaaata cattgctatg atattcaatt ccataggatt atactaaatg
    10981 tcaatagtat gaataatata ttatagatgt ttatatatca ctgttaatta ttatccacaa
    11041 taattaatga ttacctcgat atttgtcaat caaaggttga aacaatttat tttcagcatc
    11101 ttttctaact tgagctgcac tcttaataga atcatacgat cctaaatagt acctcttttt
    11161 tttaaagtta tatacgcaat ccatttctct ttagaacgat aaactccctt aacaccactt
    11221 gtattatttt tcggtaattt tgacgttaga cactctatta ctgtcccttc aacacatgtc
    11281 cttgtttctt gcctttttaa acatccacaa ctttgagtat gtccaaaaaa taagttacta
    11341 taacttatct cacacatact gccacactca cattgacaaa cccattttgt tttccatgaa
    11401 cagttattgt ccaaaggata ctttgcagta agtaatccga attttttatt ttcaatgtga
    11461 tgcttttttg atttcaaaca tccacagctt tttacatcac cttttagaag tcgctttgcc
    11521 gatactaact tttttctccc acacagacat tgacattccc aaacaacagt tccttcactt
    11581 cgcagtttag ttggcttgat tgcaactaac ttaccaaaag ttttatttgt caaattttta
    11641 tatttatgcc tatttttata atttccacat ccacaacttt tggttctacc tgatagtaag
    11701 tttgatcgat atacaatcgt ttcattccca cagtcacata ggtaacgata ttttttatat
    11761 ccattatgag taatctcctc aacaacagta aggtgaccat atctttcctt cactgcacct
    11821 accctcccaa gaaagaagta ccatataatt tcaattaact tttaataaat cagaaaaaaa
    11881 tattttcaat ttttcttagt ctgattttat gactaattta taatacaaga ctgaacgata
    11941 cgtacacgaa atcctcaaga tagataaatt gttttcatct agtaaaacct gctcaaaata
    12001 tgagcataaa gacggcaata atacactcga tattcgataa tggacttgtt ttatttgtca
    12061 aactcatcgt aatcgagata tcaattccag tctcaatatc ctgatcaaag gtctaagaat
    12121 ccaaccattt tcttgttact ttgtcagatt gattaccttt tttagtctcc gaataataag
    12181 gttttcggat caactaacat gctctgagaa agactactat ctttgcaaat ttccaaatga
    12241 aggtgtgggc cagtactatt cccagtactt cctacatagc caattatttg tccttgcaaa
    12301 acattatctc cagtttttac taggtattgt tgctggtgag catataacgc ggttgatcca
    12361 tcttcgtgtt gtatcaccac gtaatttccc caagagtagt gaaactctgc tttaacaacc
    12421 ttaccagctt taactgcatg aatcggctca ccctgtgtag ctgccatatc aagtcctcgg
    12481 tgaaattcat ttccacgatt tccaaacaaa ctggttattg aataattatg aataggcact
    12541 tggtatccag tcttactcat ttgtggacct ttaacttctt tatcatagtt ggttaaatca
    12601 taggttttaa ttaattcatt tagcttcaaa tagtattgtg tatctgttgc ataccgtcct
    12661 gtaaggaatt tagttgcttc tcgatatgtt ttactattag cttttaatgc tcctgcataa
    12721 aaatctttat ttccactaat tcccttagtt aataatttgg cataatcttc aaaactttcc
    12781 tcatagtttt catacactct aaattttgat tgggtcgtat aaagcgttcc attacccaaa
    12841 tcttcgtttg ttaccataat gacaaagttt ccattataag ttccttttat cccaaataaa
    12901 ttgtaattcg gtgcctgagc taactggctt tgaccactgg cagattctaa gatcgcttgt
    12961 gcaatcataa cagaagcata taaatcatat ttctgaccaa tttttctcgc agactcacca
    13021 attttcataa taaaattttc tacagattca tttttgtcaa aatgaatttc ttcattacca
    13081 gatagtattg gttgtgattg gaaagattct gttgattcta tttgctgtgg tgtcgaaaaa
    13141 ttttttgttt gtccttccgt ttttggcgct tccttttttg aagactctat atggtttggt
    13201 cttgtcactt tcgaagaagt tatttcactt gtactacttg aagttgttga agattcggca
    13261 cttgaaatcg tactactttc acttgacgaa gttgttgaag actcggcatt tgaagtcgta
    13321 ctactttcac ttgacgaagt tgttgaagac tcggcatttg aagtcgtact actttcactt
    13381 gatgaagttg ttgaagactc agcatttgaa gtcgtactac tttcacttga taaagttgtc
    13441 aaaagttcac tactctctgc tgttacatcg gaattactca tttcagtcgt ctcggttctt
    13501 tcttttgcaa ttttgtatgt tgaattttcg cttgccaaca catggaaagg tgttgctata
    13561 aagagttggg acattaatat aactgctgat gcaagtttta aagatttttt catttgattt
    13621 acctctcttt tttctcttta atagtgt
//
LOCUS       DAYYKN010000073        13572 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000073 DAYYKN010000000
VERSION     DAYYKN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.073"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..2265
                     /locus_tag="ACHBB7_001811"
     CDS             67..2265
                     /locus_tag="ACHBB7_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9209759.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(2847..3719)
                     /locus_tag="ACHBB7_001812"
     CDS             complement(2847..3719)
                     /locus_tag="ACHBB7_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9209760.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            4019..4993
                     /locus_tag="ACHBB7_001813"
     CDS             4019..4993
                     /locus_tag="ACHBB7_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9209761.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            5015..5707
                     /locus_tag="ACHBB7_001814"
     CDS             5015..5707
                     /locus_tag="ACHBB7_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM9209762.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            5673..7181
                     /locus_tag="ACHBB7_001815"
     CDS             5673..7181
                     /locus_tag="ACHBB7_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9209763.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            7255..8358
                     /locus_tag="ACHBB7_001816"
     CDS             7255..8358
                     /locus_tag="ACHBB7_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9209764.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            8361..9698
                     /locus_tag="ACHBB7_001817"
     CDS             8361..9698
                     /locus_tag="ACHBB7_001817"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM9209765.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            9685..10563
                     /locus_tag="ACHBB7_001818"
     CDS             9685..10563
                     /locus_tag="ACHBB7_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM9209766.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            10560..11483
                     /locus_tag="ACHBB7_001819"
     CDS             10560..11483
                     /locus_tag="ACHBB7_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM9209767.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            11480..11890
                     /locus_tag="ACHBB7_001820"
     CDS             11480..11890
                     /locus_tag="ACHBB7_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM9209768.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            11894..13462
                     /locus_tag="ACHBB7_001821"
     CDS             11894..13462
                     /locus_tag="ACHBB7_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM9209769.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
BASE COUNT         4537 a         2228 c         2861 g         3946 t
ORIGIN      
        1 attagatgag tatcaatttt acagaaaaat gaaagccttt tattttaaaa ggaggagagg
       61 aagaaaatga agaaaagtac agtattttat aaaatatttt taccgatgtt tttattagga
      121 gccgtacttg tcataggatt tagtttattc atttaccaga atacctatga atcgatcgaa
      181 tcgagttatt taacagacaa aaagaatttg ctcaggcaag ttaaaactaa cgttgaatgg
      241 aaaattagaa cgattgaata ttcgttttct acgtatggtt cgaccaaaaa tttctcggac
      301 atattcaagc atccgctgac atatacagat tactccactt attctgaaat caggaaagaa
      361 ctaaatttta tagaaacgat tgttatggat gataataatt atgatttagt cagtttggct
      421 ggtaaatggg gagtaatcaa tggatcgttg agtcaactga ccgaagagga agttgaaaag
      481 tatcaacaga agtacataaa taacaaaaat aatttatttt ggcaaaagaa aaacaaagga
      541 atcgaaatgg tgatttcttt gccaatgttt gaaaaagaaa aatatgcatt gggaattgct
      601 acgatagaga aacatacaat agaacaagtt gttggtgaaa gtggagaaga catactttct
      661 attgaaaacg aagttggcaa cctttttact actcagacag taaaaaataa agagcaacca
      721 actgtattaa cggacgtgga tctagattac aatcagctga aagtgataag agaaaaagat
      781 aaaacgtata tagtactgaa atcagattat aataattgga tttacagatt agaagttgac
      841 tctagtgcaa ttggcgcaat gatccgcaat ctgagaatcg ggctagttgc agtgtcttta
      901 acactggtag gactcattgg tcttttttcc tacttatttt cggacagatt tgcccgtccg
      961 atttctcaaa tacaggaacg tctgaatctt aaatcaaaag gattcagtgg gaaagaacta
     1021 gatttggtcg ctcaatcagt cagtcggatc ataggagaaa aagaagcgtt aagtgcacac
     1081 ttagttaccc aaaagccaca gctggaaacg ctatttgtac ttagtttatt tagaaatcga
     1141 gtagaaaggc gagaactaga tcagcggttg cagcaatttg gttatagttc tcaaaatgac
     1201 tgctattata cagctttggt tcaaatcgat ctgttagaag atagtcacgg tggggagcgt
     1261 gatttgctac ttttagcgat caacaacatg atcacggaaa ttgtgccaaa ggaaaatcga
     1321 atgctgccga tcgttttgaa tgaagaaatg caagccacta tttatcgaat ggataaagat
     1381 gatgcagaag caagcaaaaa agtcatggag tattgccgac tcattcaaaa acgtgtaaaa
     1441 gaatacttga atttaactat cagtataggc atcagtaacc ggttcgaaac gctgaatgaa
     1501 agcaagcaat cggttgatcg ggcaaaagaa gccctgtatt atcgtgtaaa tactggtcca
     1561 agttcaatta ttttttatca ggaaatcgtt cctgctgctc atgaaaaaac acttattaga
     1621 tatccgatag aagaacaaaa tcgattgttt gaagccattc gttctggaaa tcaggaagtg
     1681 aagaatttag tacatgaatt gattgatgca ttgtttgcac aaaataaaaa tcctttgagt
     1741 cgggaagtag tttcggtccg cttgatcaat gaactcgttc aattaggaca gttattggga
     1801 gtagattcaa aaaactttga taacatgaaa caaatttatg taaaagcact gaacaattat
     1861 catcccaaag aactagaaaa acttgtttta gaacaattag ttctgccaat cacagaatgc
     1921 tctcaagcaa tcactgatca tgaatttaaa aatttgtcgg agaatattat gcatatcgtc
     1981 tacacagaat atgatcgtga tctgtcttta gatgtgatcg ctgataggtt gcattataac
     2041 cctaactatt taagtaatgt atttaaaaag gaaacaggag aaaaatttgg tgattttgtt
     2101 caaaatcatc gtttagaggt agcaaaaaaa tggttgaaag agactaacct ctccgtaaaa
     2161 gagattgcag aaagattgca atatcgaaat ccgcaaaatt ttattcgttt ctttaaaaag
     2221 aaggaatcga ttacgccggg agaatatcga aaaaaatatt tgtgaatcaa gaggctgttt
     2281 ttcttgtgat ctgattcaag caataaaaga ggacagcaaa aaacagcact tcatgttttg
     2341 ctgctggctg attttattgc cgcagaatca acaagataaa cgccgtttaa tcaagaggct
     2401 gtttttcttg tggactgatt caagcaataa aagaagttag aataacccta gtatttgtat
     2461 ttatggatag ttaaatttca aggctaagca cgcaaaaaga actgtgacaa aagtcttgtc
     2521 acagttcttt tttacgaata aatggcgttc aagagacagg ctatcgacaa taaggcataa
     2581 ttttgtgaaa tatgggaagc tatttttcaa aatcgcttct tattgctcct gtgggttact
     2641 cgtcgcaatg tttgtgagag tttttatcat cgctcaaggc tgaacgtctc tttcacaacc
     2701 tctgatacac ggtgttccag gagtagattc ttcgaaaaat aagccgaaat ttcacaaaaa
     2761 tttaagaagc aattttcgta aatgccttct tatttcttga agctgaccac ttctgacaca
     2821 actaccattt tgcgttcagt acataatcac cctttgacag aacctacgag tgctcctttg
     2881 acaaaatatt tctgaacaaa aggataaaca atcagcatag gcactgttgc aataacgata
     2941 acagccattt taactgtttg tgctggtgga atcacatcaa cggcactact ttctgcttgc
     3001 atcccacttg atacaatgac gatttggcgt aaaagaatct gtatcggcca tttagatgaa
     3061 tcgttgatat acaaaatagc agtcatataa gtgttccagt aagtaacagc ataaaaaagc
     3121 gagatagtag caatagacgg taaagctaat ggcagcataa ttttgaaaaa tacaccaaaa
     3181 tctgtgcaac catccatttt tgctgattct tccaaactat ccggcaaggc ttggaaaaag
     3241 tttctcataa tgatcatatt cattgcatta acagccacag gcaggatcat ggcccagtaa
     3301 gaattgacca gccctagaga acgaacaact aagaacgttg gaatcatccc cccgctgaac
     3361 aacatagaaa atacaacaag aaagttgacg aaacctcgac caaacaaata ctttctggaa
     3421 agaccgtagg ccatcagtga agttgcacac atactgacga tcgttccgac acctgtaaca
     3481 ccaatcgaaa cagcaagtgc tctaaaaatg gtcggcgtag aaagaatgta gcgatacgcg
     3541 tctaaagaaa aggtagtagg aaaaaggata aactttttcg cgacgacttc ttgtgtagaa
     3601 gcaaaagatg tagcgatgat atttaaaaaa ggaacgatac agatcaaagc tagcaaaatt
     3661 agaaacgtat tgttgaaaag tgtaaaactt ctttctccta tagagacttt ctttttcata
     3721 tagataggct cctcctcaaa ataaattaaa tgtttctgtt tctatatgtt caatttatca
     3781 tcggtgaaag attacgtata taatcatttc ctatgctaag tgtcagatag aaaaaaatag
     3841 aaggtttgct atgcgtagtt tatttgtgaa aaaatacact ttatcgtttt gtatagcaca
     3901 aaaatcattg ctatgtaagc gtttttactt acggtaaaaa tgattatata cgtaattcag
     3961 atagttatct atcatgaagt cataagcagc aaacattcac aaaactaagg aggggcttat
     4021 ggaaagtgta aagaaacaag aaccatttca actgacgcta tcgaaaaaag aagaaaagct
     4081 cagaagacga aaaaaaataa ttgcaggaat caaaacgaat aaatttttat atgtgatgat
     4141 tctgccggga gtcctttatt tcttgatttt taggtatctt cctatggggg gactcgttat
     4201 tgcttttcaa gattatcaac catttttagg aataatggga agtccttggg tcggtttcaa
     4261 acatttcatt cgtttgttta cagaaccgac attctttaca ttattaagaa atacgttgat
     4321 cttgttcgga ttaaatatct tcgtgttttt tccgatgcca atcattttgt ctttactatt
     4381 aaatgaagta acgaacaaac gattgaaaaa cgggattcaa acgatcattt acatccctca
     4441 cttcatgtct tgggtaatca ttgtttcgat tacttatgta tttttgaatg ttgacggagg
     4501 ggtattgaat gaattccttg caacaatggg atttgaaaaa atcagttttc taacatcccc
     4561 tgaatggaca cgcacagtat atatcggaca agtgatctgg aaagagttgg gctggtctac
     4621 gatcatctac ctttcagcga taactgcagt cgatacacag ttgtatgaag ctgcagaaat
     4681 ggatggagca ggacgcttac gcaaaacatg gcatgttaca ttgccggcaa ttcgtccagt
     4741 aatcattacc ttgctgattc taaaaatcgg tcagacatta gacttagggt tcgagcatat
     4801 gtacttgtta ttgaattcat tgaatcgaag cgttgcagaa attttcgaca cctatattta
     4861 tacagctgga ttgaagaatg gacagttaag tttcagtaca gcaatgggat tgtttaaagg
     4921 ggtcgtagga ttgtttatgg taattttagc taataagatt gccaaaaagc taggagaaga
     4981 cggcgtttac taagatagaa agagagggaa agatatgctg cagtcattag ataaaataaa
     5041 cggaaggcta aatcgtattt tacagttggt ttatttgaat tttctgtggg gcggtttagt
     5101 catcgcaggt ttgggagttt ttactttcgg tcctgcatcg tatgcgatgg ttgcagtgat
     5161 tagaaaatgg ctgcgctcaa aagaaacgtt tcctttgacg aaaacgtact tcacatattt
     5221 taaagaaaac tatcgtgaaa ccttgctgat gagctggata tatggcgcca tcgggattgt
     5281 tttacttgtg gatctttctt tcatacacaa ttggtatctt aggatcgctt tgtatattgc
     5341 tgcgttcata tatgtgctct cagcactcta tattttccca atcatggctc attatgattg
     5401 gaaaggatat tttttcaaaa tgaaaatggc atttttattc ggattctcac atcttcatta
     5461 ctcattagtg ttgatattaa gtatggtagg gatttatctg atgatcatcc aaacaatgcc
     5521 aggtgtttta acctttatcg gtggcagtct cttcttttcc gcagtcacgt ggacagcaaa
     5581 ttctatattt tgccatttgg aatggaagaa ctcatataca gaaaaaacag aagaaaacaa
     5641 aaatattatt aaaacagtga aggagaaaat ttatgaaaaa agcaacgctc gtaaaattag
     5701 gtcttagttt tgcaggaatt ctagcacttg ccgcatgcgg cagcagttca agtaacagtt
     5761 cttcagagac agaagatgga aaagctaaaa tcacttggtt aaatattttg catcatgctt
     5821 caccgcctac agacacgatt gtgaacttga tcgaagaaaa aaccgatacg gaactgacat
     5881 tcaactggat tcctgatgct tcaaaagaag aaagattaac aactgcattg gcttctgatg
     5941 aactaggaga tatcgttaca cttcaaattg cgatgttgaa aaactcttct gtccgcaatg
     6001 cgttaaaatc aggcgtattc tgggatgtat cagaatattt aaaagactat aaaaatttaa
     6061 gtcaaatttc tgaagataga atcgaagctg cgagtatcga cggtgccttg tacggcgtac
     6121 cgatacaaaa agactacgct cgtgctggct tagtcattcg aaaagactgg ttggataatc
     6181 taggactaga tgttccaact accatggatg agctttatga agtagctaaa caatttacag
     6241 aaaacgatcc tgatggaaat ggtgtggacg acacagttgg ttttggcgat cgtgcgccta
     6301 gtgatattcg ttataccagc tttaaacttt tcacttctta ttttggagca ccaaatggct
     6361 gggctgtaga cgataatggc aaattcactc cagaatttga tacagaagag tatatgaaag
     6421 ctttgaattt ttcaagagat ctatttgaga atggctattt ggcacaagac tttgcagtta
     6481 cccaaaagac tgatcagcaa gaacaatttg cacaaggaag aactggaatc tacactggaa
     6541 tggtggatat caagaatctg aagacgatgg gtcaaggtat tcaagatgat atggaattag
     6601 tgcctgtaaa caagatctca gatggtgaag gcggcggtta tcatgtttgg tcagaaggaa
     6661 atggtattgg cggcttgcta gcatttccca aatctcaagt aaaagacgaa gcacaattga
     6721 agaaattact tcaatttgtt gatgatcttt tagaagaaga tgtgtataaa ttgatgactg
     6781 gcggtatcga aggaacgcat tatgaattga atgatgatgg ttctatcgaa tttatcgata
     6841 tggatctttg gcaacaagag gtgcaaccgt tatcaagctc tcgtcctagt gaagtgacgt
     6901 tctctttcaa agatacagac cctgaaaaag aattctctaa tcaattgatc aaagaaaatg
     6961 aagaatttgc tgtaatggat ccttctgtgc cattggattc accgacgaac aatgaagtag
     7021 gatcagaaat tgaaaaaatc gtcattgatg ctacggtgca atacatcatg ggacagcttg
     7081 acgaagatgg attcaaaaaa gctgtagaag actggaaagc acaaggaggc gatcgaatca
     7141 cacaagagta cgaggaagca tacaaagcag cggcacagta atatagacga ttgggacaaa
     7201 tacttcttgg tatttgtccc aaatcctatt ttaattttta aaggagagca aataatggaa
     7261 aactggtcaa agaaattagc aaaaagtatc atggaaagaa cacctcgctt atatgaagaa
     7321 aagtggtata aaggaaaatg gtcttatgac tatggcgtcg tacttaaagg gttccaatta
     7381 ctttgggaac gaacccaaga aaaaatttat tttgatttca taaaagacaa tatcgactat
     7441 ttcgtgcaag aagatggaac gatccgtggc tacagcgttg aagaatacaa tatcgaccat
     7501 gtaaatacag gaaagctttt ctttttattg tacaaagaaa caggcgaaga aaaatataaa
     7561 aaagcagcag aactcctttc ccagcagttg gcaaaccatc cacgcacatc cgaaggagct
     7621 ttttggcata aagaaattta tccttatcaa atttggttgg atggcttata tatgggttct
     7681 cctttttatg ctgaatttat ccagaatttt agtgaagaaa aagattacca agatgttttg
     7741 agacagtttg aaatcagtta taaacatcta ttcgatccaa aaacaaattt gctgattcat
     7801 gcatgggatg aaaagaaggt ccaaccatgg gctgatccaa aaacaggtct atcccatcat
     7861 ttttggagcc gatcgattgg ctggtatttg atggctgcag cagataccta tgaattactt
     7921 catgaagaaa cagattgttc tttgctgaaa gaaattctag aaaagacact ggctgcatta
     7981 cttccatatc aagaagaaaa cagcgggaca tggtatcaag taacaacaga aacaaaacgg
     8041 aaaggaaatt atctggaagc ttccggcagt agtatgtttg tttacgcaat ggctaaagga
     8101 atacgtttgg gactgctgaa caaagaagaa tggcttccac aagtgaagaa atcacatcaa
     8161 ggattacttg atgaatttgt tctggaaaca aaagaaggct ggctgaactt gaataaaaac
     8221 tgtcaagtag cagggttagg cggagcagat cagcgagatg gttcatatgc ttactacatc
     8281 agtgaaccaa ttatttgtaa tgatcaaaaa ggcgttggtg cattcttgca agcattgatt
     8341 gaagtggagg cattataaat atgtatgata ttttgacatt tggcgcatca attgatgaat
     8401 tgaatactga agcaatacaa caagccattg atgctgcagc aagtgacgga ggcgggaccg
     8461 tagttgttcc agcaggtgaa tttctaacag gagctttatt tttaaaaagc aatgtcgaac
     8521 ttcatttaag cgcaggagcg gttctgaaat tctctgacga tccaaaagat tatcctgttg
     8581 ttcattcacg ttgggaagga gtccatcgaa aagtttatgc ttcctgtatc tatgctcaaa
     8641 atgtagagaa catctctgtc actggtttcg gtacattaga tggaaatgga aaaaaatggt
     8701 ggcatacttt tagaaatgaa ccagataatc tcgcttatcc tcgtcctaaa ttgatgagtt
     8761 tccataactg tcatcgaatt actgtgaaag atattaaatt gatccagtca ccaagttgga
     8821 cgatcaaccc gattctttgc agtaatgcaa cgtttgataa cttgacgatc ttgaacccag
     8881 cggattcacc aaatacagac ggaattgatc cagaatcatg caaaaatgtg cgtattagta
     8941 attgccatat tgacgtggga gatgactgta ttgcaatcaa ggcagggact gaagatacgt
     9001 acgaaaggat cgcttgcgaa aatatcacga tcacaaattg cacgatggtc catggacatg
     9061 gcggtgtcgt attaggaagt gaaatgagtg gtagtattcg taatattacc atttcaaatt
     9121 gtatcttcca agaaactgat cgaggaatac gattgaaatc tagacgaggt cgaggcggga
     9181 tcgttgaaga tattcgagta tccaatatag tcatggataa tgtgatgtgt ccatttatac
     9241 tgaatcttta ttatttctgt ggtccgcgag ggaaagaacc gtatgtatgg gaaaaaaagg
     9301 cttatccgat agatgaacgg acaccagcat tcagaaggat ccatttttcg aatatcacgg
     9361 caaggaatgt ccatgcttct gcaggtttta tttatggatt agcggaacaa ttcatacaag
     9421 aaatcacatt tgacaatatc gatgtctcga tggcccaaga tgcagttccc ggtgttcctg
     9481 ctatgatgac aggaatagag gaaatgagca gacaaggatt ttatattggc tttgcgggta
     9541 aggtttcctt taaccagatg tcgattgaaa atcacgaggg accagcattt ctattagaaa
     9601 atactaaaga aatcagtatt gagaaattcc aatcaaatca atcagaaaat atggatgaac
     9661 tggttgttcg taaaaacttt gcttatgcag ttgaatgatt ttttaggtgc tccatctagc
     9721 agacataccc ctagtggaaa acgattatac cgagaaaaac aaaacggttt tatgcttgaa
     9781 tactatcaat tgtacttaaa tgaattggaa acagtaccag ccctctatgc ttatccagaa
     9841 aaagaaggac cgtttccgcc ggtcctttac ctgcattctc acggcggaga tttttcagtc
     9901 gggaaaagtg aattgatcca tggtgctcct tacttagctt ctccatcttt tgcagaagta
     9961 ctgacaggaa tgggatatgc tgtttggtcg attgatgctt ggggatttga agaacgcgga
    10021 ggtatttctg aaagcgagtt gttcaaacaa tttttattaa caggaaaaac gttatgggga
    10081 atgcgtattt atgatgtgat cagcttgatc gattatttgg agacaaggga agacacagat
    10141 actcaacggc tcgcaacgat tggtctttct atgggaggct tattaagctg gtgggtgaca
    10201 gcattggatt ctcgtgtcaa agtttgtatt gatctagcgg cacaggtgga catcgaaata
    10261 cttttgctac atcgccgcct agatcatcat gggttttatt actatgtgcc aaatctattg
    10321 acagcttata caacgttagc aatccaagaa aaaatcgctc cgcgccctcg tcttagctta
    10381 gttggaaaaa acgatacgat gtgcttggac gaaggcgtac tgaagctgag aaaagggctg
    10441 gacaaaaagt atcaatcaat gggtagtcca gaaaactttt cttctttttc tttaacaggc
    10501 ggtcatatgg aaacacaaga aatgagaaat atttggaaac aattcataag ggagcattta
    10561 tgaggatgaa agacaaactg ataattggaa agaatgctat agataccccg acggattttg
    10621 aaactatcca agaaggaatc gattatttgg caggcctgcc ggatagccat ccaaaggtct
    10681 tgatagtaaa agaaggaaga tatgatgaat atatagaaag cagattatcc aattttaaac
    10741 ttgtgggagc aggaacagta gttatctccg gtaatcgttt tgcaaaacag gagtatcttg
    10801 acggtagcca gttagggact ttccgaagtg ctactttttt cttagaagga tcgaatgtcg
    10861 tattagaaaa tcttcacatc gaaaatacag caggtccagg agaaaaggta ggacaggctg
    10921 ttgctttatt caattcagga gatcgcgtat ctttaaaaaa ctgtcgacta tctggttatc
    10981 aggatacctt atgcacgggt ccgttgcctg atttgcaaaa agatggtact cctttttgta
    11041 caccggcttc tgccaatcct gagtattgtc gtcagaccta tgaatattgc tggattgaag
    11101 gaacggtgga tttcatattt ggtggggcag atgccgtttt tcatcagtgt catatccata
    11161 gcttagccag taaaaatcct ggatacatca cagcagcatc tacgccgaaa aatcaaaaaa
    11221 cagggtatcg ttttgaaagc tgtaccttga ctgcagaacc tgatactaga aacgtctatt
    11281 taggaagacc gtggcgtccg tatgcaaatg tcttatttga agcgtgtaag ataggtgggc
    11341 atgttcaccc aactggttgg gataattggg atgacaagaa aaatgaacag actgtcaaat
    11401 tctctgaacg taataacaat tatgaaacat cgatcaaaag acctgaatgg atacatttag
    11461 aaaagcaggg ggttcagaaa tgaaaagaaa atgggaacta ttgctaggaa tggtcggtgg
    11521 cagtctatct ttgattttct ttggcgggtt agcagttact ttaagtaata tgtctgcgtc
    11581 agagtttaaa aaaagttatc agtcgcttgc tgtagatcat tcaactttgt ctttagaaaa
    11641 tacatttgga ttattacagg acatgactgg tctgtttgcc gttgtgttgt ttatttcact
    11701 tgctttttta gcagtagcat tgtttttgac tgctaaagga aaatacttaa caactgccac
    11761 tggtttgtat tttataacag gattcatatt gttgattggc actcagttta ttgcgtttcc
    11821 ttttgcattt ttttatttcg cagctggggc attcagttta tatcgagtaa gaatgagaaa
    11881 aggagcctga atcatgcaga ctatcaaagt gaaaaatcct attttaagag gattcaatcc
    11941 agatcctaat atattaagag tgaaagagcg gtattacatt attgtctcct cttttgaatg
    12001 gcttccagga atccgagtat atgagtcgaa aaatttagtc aactggtcac atacaacgga
    12061 tattttgacc actcaagtat ctttgcaagg aaatcctaca aatggaagta tatgggcgcc
    12121 acaaatcagt tatgctgatg gattgtttta tcttgtttac acagatgtca aaagtgcagg
    12181 ccgtccgttt aaagatgtcc acaattattt gatcactgca tctgagatca caggtccttg
    12241 gtccgaacca atttatttaa atagtaacgg ctttgatcct tctttgttcc atgatcttga
    12301 aacgggaaaa aaatggcttg tcaatgaact atgggattat cgtctggaca caccgaataa
    12361 atccagcggt atcgtattac aagaatatga tgcagagaag aagaagcttg atggtcccat
    12421 ctataaaata tttgcaggta cagaattagc gaaaacggag gcaccacatt tgtattttcg
    12481 taacgggtat tattatttga tgacggcaga aggcggtaca ggaagaggac attcagttac
    12541 agtttgccga gcaaagaata ttactggtcc ttacgagctt gatcctggtt ttcctatgct
    12601 gacagctagt gacaaacctg cttctacatt gcaatgtaca ggacatggga gcttagtaca
    12661 aaccccgtca ggaagatggt acatgacata tctttgtacc agaccattaa acggtgcggc
    12721 tattttggga agagaaaccg ctattcaaga agtgtattgg acgtccgacg attggttaag
    12781 acttaaagat agccagacaa ctccgatgga agaaataatg attgagacaa atgaagaggt
    12841 acaacaagaa aaagatcatc agtttatgga tacatttgaa ggagagctaa agaagaactg
    12901 gaatgccaga cgcattttgc caaatgctga ttggtgtgat acaaaatgta gaccaggttt
    12961 tttacgtctg atttccggtg aatctcttca atctaccttc gaacatcatt tgttagctat
    13021 tcggcaaacg gattttactt ttgatgcgga gacagcattt gaatacatgc ctaataattt
    13081 taatcagatg gctggactgg tactgtactt aaatgaatcc aattatattt attgttatgt
    13141 tacttggaat gaggacaagg gaaaatgcct acgtatgata gaatcagaaa acggagtagc
    13201 acaaatagaa gattggacga ttccactcac agaaaagaag acttttttga aagtcactgt
    13261 aaagaaagag acagcacagt ttcatttgaa agacgtatca actgaagaat ggaagaaagt
    13321 aggtccgcaa aaaaacatgt gtatactctc aggagggttt actggaaatt ttatcgggat
    13381 agcagtgcat gatctaaata aaaaaagagg aagttatgct gattttgagt ttttcaagta
    13441 tgatggaaaa gatcatctgt aatttaattc tttaataaat gaggttcact aacttatttt
    13501 cactagttag tgaatttttt tgtttgtata tattgttatg taaaaaaagt tatcaatctt
    13561 atgcatcaat gt
//
LOCUS       DAYYKN010000074        13404 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000074 DAYYKN010000000
VERSION     DAYYKN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.074"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            333..947
                     /gene="gmk"
                     /locus_tag="ACHBB7_001822"
     CDS             333..947
                     /gene="gmk"
                     /locus_tag="ACHBB7_001822"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM9209770.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            952..1257
                     /gene="rpoZ"
                     /locus_tag="ACHBB7_001823"
     CDS             952..1257
                     /gene="rpoZ"
                     /locus_tag="ACHBB7_001823"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9209771.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1510..3918
                     /gene="priA"
                     /locus_tag="ACHBB7_001824"
     CDS             1510..3918
                     /gene="priA"
                     /locus_tag="ACHBB7_001824"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM9209772.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3933..4424
                     /gene="def"
                     /locus_tag="ACHBB7_001825"
     CDS             3933..4424
                     /gene="def"
                     /locus_tag="ACHBB7_001825"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9209773.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4417..5355
                     /gene="fmt"
                     /locus_tag="ACHBB7_001826"
     CDS             4417..5355
                     /gene="fmt"
                     /locus_tag="ACHBB7_001826"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM9209774.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5355..6713
                     /gene="rsmB"
                     /locus_tag="ACHBB7_001827"
     CDS             5355..6713
                     /gene="rsmB"
                     /locus_tag="ACHBB7_001827"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM9209775.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6726..7466
                     /locus_tag="ACHBB7_001828"
     CDS             6726..7466
                     /locus_tag="ACHBB7_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM9209776.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7463..9532
                     /gene="pknB"
                     /locus_tag="ACHBB7_001829"
     CDS             7463..9532
                     /gene="pknB"
                     /locus_tag="ACHBB7_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM9209777.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9697..10596
                     /gene="rsgA"
                     /locus_tag="ACHBB7_001830"
     CDS             9697..10596
                     /gene="rsgA"
                     /locus_tag="ACHBB7_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM9209778.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10609..11259
                     /gene="rpe"
                     /locus_tag="ACHBB7_001831"
     CDS             10609..11259
                     /gene="rpe"
                     /locus_tag="ACHBB7_001831"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM9209779.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11260..11898
                     /locus_tag="ACHBB7_001832"
     CDS             11260..11898
                     /locus_tag="ACHBB7_001832"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM9209780.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12053..12907
                     /gene="mreC"
                     /locus_tag="ACHBB7_001833"
     CDS             12053..12907
                     /gene="mreC"
                     /locus_tag="ACHBB7_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM9209781.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12911..>13404
                     /gene="mreD"
                     /locus_tag="ACHBB7_001834"
     CDS             12911..>13404
                     /gene="mreD"
                     /locus_tag="ACHBB7_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM9209782.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKK"
BASE COUNT         4429 a         2478 c         2963 g         3534 t
ORIGIN      
        1 gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt tttcttcata
       61 ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag cttgcatctg
      121 cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat
      181 tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt tttaccaatt
      241 tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata
      301 agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt
      361 tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt gagagtgagg
      421 ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg
      481 aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc gaagctggag
      541 agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg tcatatatca
      601 acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa ggagcaaaac
      661 aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca gatttagcgg
      721 aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc gaagaacgaa
      781 tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct gttgtaaatg
      841 atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa cattttcgag
      901 tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct tatgatgtta
      961 aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct agtgatttta
     1021 gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga ttcttttgat
     1081 tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt agtcaatgat
     1141 ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg tcaagcacaa
     1201 aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa aatgtaataa
     1261 agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct tttcaaacat
     1321 tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca gttctttttt
     1381 ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa atccatcgat
     1441 ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa aaaaaggagg
     1501 aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat gcagaccgat
     1561 cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt cgggatgcga
     1621 gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat ggctatccaa
     1681 aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct agaccttgct
     1741 ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat tacttacgca
     1801 ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga atatgataaa
     1861 ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca gcgagaagtg
     1921 atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg ctggcgccaa
     1981 gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa aacgattcgt
     2041 ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa
     2101 aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca agaagaacca
     2161 attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg aaaagaaaag
     2221 ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga ccgagtattt
     2281 gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga acaaatcatc
     2341 acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac tggcagcggg
     2401 aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa aacggctatt
     2461 atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa aggacgcttc
     2521 ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata tgatgaatgg
     2581 cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc tatttttgcc
     2641 ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag ttacaaacaa
     2701 gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa atatcatcat
     2761 tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg agctcaaaaa
     2821 ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca gcttccagct
     2881 attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac tttttctgct
     2941 aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt cctactgctc
     3001 aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt tcttccatgt
     3061 cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg ctgccattat
     3121 tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc
     3181 tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta tccgcaagct
     3241 aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga acagatttta
     3301 caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat tgctaaaggt
     3361 ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc cctgaattta
     3421 ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc aggacgagca
     3481 gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca acattacgcc
     3541 atagaattag ctaaaaaaca aaattatgag cagttttatc agcaggaaat gcatgtacgg
     3601 catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag ccatcctgaa
     3661 gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga agtgttaagc
     3721 cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa aaaccgatat
     3781 tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt tttggatgaa
     3841 attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat tgacaacgaa
     3901 ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg atccacccta
     3961 atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag acaatcgctt
     4021 tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc gctgctcctc
     4081 aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag aaatttgaac
     4141 tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca
     4201 gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg cgttattatg
     4261 atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt gccttccagc
     4321 atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag cagatcccgg
     4381 aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt atttatgggc
     4441 acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta cgagatccag
     4501 gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac gccgacaccg
     4561 gtaaaagaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa aatctccggt
     4621 tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac tgcggcattt
     4681 ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat caatgtccat
     4741 gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat catgaatggg
     4801 gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc aggcggaatc
     4861 tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat gtttgaaaag
     4921 cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt ggatggacaa
     4981 aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac acgggaacaa
     5041 gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg cggaatgcgt
     5101 ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt atcggttgaa
     5161 gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga caaaaaaaat
     5221 ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca gccagctggt
     5281 aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt tcagattggt
     5341 cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg
     5401 cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg
     5461 agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca gaattgatct
     5521 atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg atcgctaaag
     5581 caaaaaaagt ggatgactgg gtcaagacgc ttctttatct ttctgtatac caattagaat
     5641 ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa
     5701 aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat cagcgtcaag
     5761 gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc aaaatcagcc
     5821 tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa actgaaaagc
     5881 tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca cgccgacttt
     5941 ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa aaaagccaag
     6001 tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca ttgttccacg
     6061 atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg
     6121 agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg acgcatattg
     6181 cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta cacgaacaca
     6241 aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct gtctacgctc
     6301 aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt gatcggattt
     6361 tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat attcgttata
     6421 aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt ttagaaagct
     6481 gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg atcttaaaag
     6541 aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt gagaggattg
     6601 atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg actttgtatc
     6661 cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag taatgaggtg
     6721 aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca aatcaagact
     6781 atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca gacgggatgg
     6841 gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta ggcgaagctt
     6901 gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt caatcgattc
     6961 aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat aacggtatgg
     7021 gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc aatgttgggg
     7081 atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag gatcattctt
     7141 tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa
     7201 ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta gatgtttcta
     7261 ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg acgaacatgc
     7321 tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct cataaagtaa
     7381 ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc gttcttctta
     7441 tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg gccgttatca
     7501 aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc acgatttaat
     7561 cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg accagactgc
     7621 gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc caaacattgt
     7681 tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg aatacgtcaa
     7741 aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg aaacagcagt
     7801 aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc aaatcatcca
     7861 ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa aaatcacgga
     7921 tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata cgatgctggg
     7981 atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga agcagtcaga
     8041 tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac cgtttgatgg
     8101 tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat ctataaaagc
     8161 acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa cggctaaaga
     8221 acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta cagtattatc
     8281 accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg aaacaaaagt
     8341 tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta tgcctctgcc
     8401 aaaagacaag ccagaaaacg aaaccttgcc agaggaggaa gaagttctgc ctgatcctaa
     8461 aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg ttttaggaat
     8521 cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc
     8581 tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag aagtagcaac
     8641 aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga agacagaccc
     8701 ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta gtagcggcac
     8761 gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg aagcaagttt
     8821 gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca gttcagaggt
     8881 agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg atcctaaaac
     8941 agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga gtagtcatgt
     9001 aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca atatcaataa
     9061 tattagtgct acttatgact atagcgacac tgtaccatca ggttcgatca tcagtcagga
     9121 tccagcctcg ggtgaagttg tagcagaaga aacttacatc aattttgtcg taagccaagg
     9181 aacagagcct aaattgaatg atatctccgg ctatacaaaa agcgaagcac aaagttattt
     9241 agctagtgtc ggtgcagaat atatcgggca tgagacttcc gaatattcaa acacggtgga
     9301 taaagataaa gtgattcgta cagtaccagg ggcaggaacg acaattacca aaggaatggt
     9361 tgtgaacgta atttactcta aaggaccaga tccagcgtct tctagttctg aaaaagactc
     9421 ttcagacagt tctagcagct caagtagctc cagtagcaca agcaactctt caaaagaaga
     9481 atcttccagc tccacaagtt catcagctag cacctcagaa agttcaaaat aagtgctgaa
     9541 taaaatatat cagaagggaa tgcgacattg atttgttgca ttctcttttt tgtgtttcta
     9601 tctcttttga tcaattttca agccatatac tttgagaagt ttgcatggaa taacacctac
     9661 tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg cacttatcaa aggacaaatc
     9721 agaaaagcaa tcagcggttt ttactatgta tacgcaaacg gagagaccta tcaaacaaga
     9781 ggaagaggaa atttccggaa tcggaaaatc acgccgcttg tcggagatca ggtgatcttt
     9841 gagagtgaaa atcaaacaga cggctatctt ctggaaatct tgccgagaaa aaatcaatta
     9901 gttcgcccac cagtcacaaa tgttgatctt ggaatcattg tcacaagtat cgtagagcct
     9961 gcattttctt ataatctact tgaccgcttt ctagttacct tggaatacga gtcgatcgaa
    10021 ccgattattt ttttgacaaa gaccgacttg tcagacgacc cagaaaaaat cgaagagatc
    10081 aagcaaacct atactaaaat aggctatccg gtgattgtac ccaatcatac gggtgatacg
    10141 acagaactga tccgttactt cccagaacgg ctatcggtct ttatgggtca gtctggagca
    10201 ggaaaatcga cgctactgaa tcaaatatca ccggaactga atttagcaac aggagagatt
    10261 tctgatgcac ttggaagagg acggcataca acacgtcatg tggaactgat tcctatctat
    10321 ggcgggttag tagcagatac accaggattt agctcgattg actttttgac aattgaagca
    10381 ggagaactgc cgagacaatt tcctgaattt gtagaagctt ctgtccattg caaatttaga
    10441 gagtgcatgc atgctaaaga acctggctgc gaagtaaaac atagagtaga aacaggtggt
    10501 atcgcacaga cacgttacga caattatttg caattcttac aagaaatcga aaaaagaaaa
    10561 ccaatgtatg caaaaaaaga aggtaaacga aaataggagg aaacaagaat gaagatagca
    10621 ccatccattt tgagtgcaga ttttgccaat ttacaaagag acattgagtt agtggaaaaa
    10681 ggaggcgcag attacatcca cgtagatgta atggatggcc aatttgtccc aaatatcaca
    10741 ttagggccaa atattgttca ggcaatccgt cctgttacta aattaccgtt agacgtccat
    10801 ctgatgatcg ttgatccaga aaactacatt ccagcatttg caaaagctgg agcagatatc
    10861 attactgtcc acgtcgaagc aactcctcat attcatcggg cacttcaaat gacaaaaagc
    10921 ttaggagtta aagctggagt agttatcaat ccaggaacac cagtctccat gatcaaacat
    10981 gttttgcctt tggctgatca agtactagtc atgactgtca atcctgggtt cggcggacaa
    11041 agctttattg aagaaacagt agaaaaaatt acggaactcg ctgatctacg tagagagaac
    11101 aattggactt atgcaatcga agtagatggc ggaatcgttc cagaaacagc aaaaatctgt
    11161 aaagatgcag gggcggatgt cttcgttgca ggatcatata tctatggagc cgaaaatcca
    11221 gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa tgaaaatttt attagctgct
    11281 ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg
    11341 atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc cagtagatct ggcagtgggc
    11401 gattttgatt cattgtcgaa cgaagagcag aaggaagtat ttgcaaaagc caaagagtat
    11461 acccgttccc ctgcagaaaa agacgataca gatacacagt taggtatctt aaaaacattt
    11521 gaacgttacc cgcaagcgga agtcactttg attggagcta cgggcggaag attggatcat
    11581 cttttagcca atctctggtt aggtatggaa cctcgcttca aaccctttgc ccatcagctt
    11641 attatcagag attgtcaaaa ttatttgtct tattatttgc ctggagagca tatagtaaga
    11701 cagataccag atatgaagta tttagcttat tgctgcttga ctcccgtctc gaatcttaca
    11761 ttaaaaggca gcaagtatgt attggaaaat aaagaagtag aatatccgac atcttatgca
    11821 agtaacgaat ttctttcaga taccgcagaa ttttcttttt cagatggtat gattgcagtt
    11881 attcaaagca aagattgatc cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg
    11941 acagaatcta ttatttgcta aaaaaaagct taagaggagc aacttacctt ttgtttcacg
    12001 gcatttagtg ataaaattga acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa
    12061 gttcaatcct aataaaaata ttattattac cttggttatt gttatcatcg tggtgacgat
    12121 actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag cccaatttct tccaatccat
    12181 cgttaatgac agtgttgcaa tggttgataa aacaatctct gccccagtac ggtgggtacg
    12241 aaatggagta gattctgtcc ataatctttt ttcaacttat gccgaaaatg agcggctaaa
    12301 agaaaaaatc gatagctatg atgacgtttc acagcaaaac aagaattacg agagagaaat
    12361 cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg actagttacg aaaaagtaac
    12421 ggcaaatgtc attactcgtt cgcctgacag ttggcaggac atgctgatcg tagacaaggg
    12481 ttctaaagac ggtatcgaag caaatatggc agtcatgtcg caaaaaggat tgattggccg
    12541 agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag
    12601 ctccaatcat tttccagtac gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa
    12661 aaactatgat gaaaagctcc atgccttagt ggtgacccaa ttaactggtg atacggatat
    12721 caaagaaggg gatgttgtcc agacatccgg tcttggaggg aattctccag ctaacttgcc
    12781 gatcggtacg gttattaaaa cgaaaccaga tagttatggg ctggatcggg aagtttatgt
    12841 gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg attgtccaac ggctagcgga
    12901 ggattagtcc atgttgaaga aagaaacaat gaagtactat ctgccaatcg ttttgttctt
    12961 tttgatgttg atagatggtc atttaacaag aatgctaggg gagtggtcga aaggcaccta
    13021 tatgtcaaat gcccactttc tgatattggc attattatgt tgcagtatgg cgtttgaaaa
    13081 acgttattta ctgattacca cgattgttct cggggctatc tatgacgctt actatattgg
    13141 cgttatcggt atctatgcag tagctctccc tttaattgta tggttgatgt atgtaatgaa
    13201 agacgttatc catgtcaaca tctttactga atttttcagt atgatcatct ttgtcacggg
    13261 ttatgaattg tttacgatgg tggtccagtt gatttttaaa ttagcagtag taaataacac
    13321 gtattttatt acaaggtttt taggacctac actgctgttg aacatgatta tatttgtatt
    13381 attcattttt ccctttaaga aatt
//
LOCUS       DAYYKN010000075        13234 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000075 DAYYKN010000000
VERSION     DAYYKN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.075"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..435)
                     /locus_tag="ACHBB7_001835"
     CDS             complement(<1..435)
                     /locus_tag="ACHBB7_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209783.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSG"
     gene            complement(446..1114)
                     /locus_tag="ACHBB7_001836"
     CDS             complement(446..1114)
                     /locus_tag="ACHBB7_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFM9209784.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1245..1829)
                     /locus_tag="ACHBB7_001837"
     CDS             complement(1245..1829)
                     /locus_tag="ACHBB7_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9209785.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2164..2394)
                     /locus_tag="ACHBB7_001838"
     CDS             complement(2164..2394)
                     /locus_tag="ACHBB7_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM9209786.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(2409..3359)
                     /locus_tag="ACHBB7_001839"
     CDS             complement(2409..3359)
                     /locus_tag="ACHBB7_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM9209787.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3356..4198)
                     /locus_tag="ACHBB7_001840"
     CDS             complement(3356..4198)
                     /locus_tag="ACHBB7_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM9209788.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(4331..5056)
                     /locus_tag="ACHBB7_001841"
     CDS             complement(4331..5056)
                     /locus_tag="ACHBB7_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9209789.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(5517..5831)
                     /locus_tag="ACHBB7_001842"
     CDS             complement(5517..5831)
                     /locus_tag="ACHBB7_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM9209790.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(5847..7442)
                     /locus_tag="ACHBB7_001843"
     CDS             complement(5847..7442)
                     /locus_tag="ACHBB7_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFM9209791.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(7465..8355)
                     /gene="iolE"
                     /locus_tag="ACHBB7_001844"
     CDS             complement(7465..8355)
                     /gene="iolE"
                     /locus_tag="ACHBB7_001844"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFM9209792.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(8372..9397)
                     /gene="iolG"
                     /locus_tag="ACHBB7_001845"
     CDS             complement(8372..9397)
                     /gene="iolG"
                     /locus_tag="ACHBB7_001845"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFM9209793.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(9421..10428)
                     /locus_tag="ACHBB7_001846"
     CDS             complement(9421..10428)
                     /locus_tag="ACHBB7_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9209794.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(10541..11701)
                     /locus_tag="ACHBB7_001847"
     CDS             complement(10541..11701)
                     /locus_tag="ACHBB7_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFM9209795.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(11777..13231)
                     /locus_tag="ACHBB7_001848"
     CDS             complement(11777..13231)
                     /locus_tag="ACHBB7_001848"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFM9209796.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         3953 a         2868 c         2174 g         4239 t
ORIGIN      
        1 accacttgat agcgaaaaaa tgtttttatc taatatattt gaaagatcta ataattcagc
       61 taattcattg attcttttat ccatatgctc ttttgtaaat ccaaagtttt ccatcgaaaa
      121 tactaactca ctcgttgtat ttgttgtaaa gaattgtcct ctaggatctt gacttacgta
      181 gccaatattt tttgatatac tttcttgtgt taaggattgt agcgagttat tttcaaataa
      241 aatttcccca accaattttc cgtcataaag tcttggaata agtccattca ttaactttaa
      301 tagtgtggat ttcccactcc cagaagaacc agtaatcaat gtaagactac ctttttttat
      361 tgaaaaatta aggtcaacaa gcgcttgttc cttaacatcc ttatatgaaa aagaaacatt
      421 gttaaattca aacaattctt tcacctcaaa tcaaaatact taagaaaaaa atagtaacgg
      481 aaaacaggat gatatagtct aataagctca tgtttgtttt atagtaagaa cttcttttca
      541 caggagattc tatccccttt gttattgcat tagcggtcat ttcttcagca atcatagttg
      601 cacgtataag aacgggaact aacagaaatt ccaaatattg cagaggctga aaaaaagaca
      661 agcgaatgtt tctcattttt aaagattctt taatcattct tatttcagaa cgataagtcg
      721 gaaagaatct gatcactact gtaatactta acattatttt tttcggtaaa tgtatttttt
      781 ctaaagagga taataattca ttgctagtat agacactaac caacaaccat gagatcatta
      841 cgaccggcaa cattcgaaat acaatatata aggatgtccc aaaaaatata gtaaaaaact
      901 ctatcttgat agcatcaata attttcatta ctaaaaacag aactgaataa atcaagatga
      961 aataaataca tgtctttatt ttacgaaaca tcaatactaa aactgtagca ataatgaaac
     1021 accccacttg aaatatattt ccctttccta aaaaaataat gaccggaaaa aaaatattta
     1081 aagtaatcaa ggttaacgga ttgatttttg acactctttc acctccaaaa aataagatcg
     1141 cactgttatc ttaagctgat aaaaatgcac cgttattcag ataacagtgc gatcttatca
     1201 aaaagcataa ctgagaacaa ttctcaatgc aagctttttt ttaattaact cagtaattca
     1261 atccacccag gaacagaaaa ttgatacaat cttctggtta atagcatagt cttttctttt
     1321 gaatagctat gcttcaatat atcatagaat gtagcgtaga atgagtaaat gtagaagtgg
     1381 atttcttctt cattaaagac taaattttga ttgatcattc cgatagtctt taacttatct
     1441 agacaacaga ctgtcttttt tgtcacaaat gatgtaattt tttcaacaat atcttgatat
     1501 ttggttccag ctgattggaa taaaaccaaa tcgaatatac gtttattctc aaaaatataa
     1561 ttaataaatg gatctaattc attgaagttc tctctccaac ttttctctat atctatattt
     1621 tctaagttta agaggtattt ggtaaactct tcattataca tattaataag cgcattaata
     1681 tgcggctgaa taatttcttc aaataacgct tccttagaag taaaatgttt ataaaaagca
     1741 cccgtagtta cgcctgcggt caagcacagt tgccttatct tagtttgctg aaatcctact
     1801 ttttcaaaat ggcttagtcc actttctatt aatttttggt gagttgtaat ataatctctg
     1861 gtttctatgg gtacttcctc ctaataagat gaattaacta tattatctta cacaggactt
     1921 gaagtttcaa tcgttaacgt ctattactga actcattcct tagagctaaa ctaatgagaa
     1981 agagcaaaat aactgataca aaaagcaatc ctacaagaat taatagactc atctaaatca
     2041 ttcacctttt ctaaaataat ccgagaaaga tttaaaaaaa ttatatccag ctattaggat
     2101 aagtcctatc ataatcactc caatagccat tttcttatcc tccctaaaaa taaagccttt
     2161 tacttaaaac ctttttttgt aatcgtcaat aaattttttc cctttatcag ttaattgaaa
     2221 tcctatatct aaatagtcct catatctaag tttagtcagc atctcactta cagatgctgg
     2281 actaacactt agaacttttg ctaaatcatt attttttaat ttcttttttc tttttgataa
     2341 ttcctgaatt gctagtaaat aaattagtat agtagttgtt ttaatttgta ccatacctac
     2401 cctccaaatt atttcgaaag gccttcagca attttatcca ggttccattt catcatcgca
     2461 tagtaactat caccatcttg tcctttttct gcaattgaat cagtaaaaat cgttgaatag
     2521 ataggaatat tggtatcttt tgatactgtt ttcattggtc tatcgtccac actgctttct
     2581 acgaataagg agggaacttt tgttgtgcgt aatttttcaa ctaagtgttt tatttgatct
     2641 ggtgttcctt cttcttcagt gttgatttcc caaatgtaag cagaaggcac attatacgct
     2701 ttcgagaaat atttaaagca tccttcactt gtaacaatca tttttttatc attcggaatt
     2761 gaagcaaatt tagatttagc ttctttgtct agagaatcca atttttcaat atacttatct
     2821 agattttctt tatagaattt tttattatca ggatcttttt ccgctaactg tttttcaata
     2881 tttttagcgt aaataatacc gttttctaaa tttaaccaag catggggatc ttccttccct
     2941 ttctcactct gaccctctaa gtaaataaca tctatgccat cacttgctgc aaaatagtct
     3001 ttgttttcct ctttgttcgc attttttact aattttgtaa accaagcatt tcctccagtt
     3061 tccaagttaa caccgttata aaaaatcaaa tctgcttttg aagttttttg aacatcttca
     3121 ggcaaaggtt catattcgtg gggatctttc ccaataggta cgatactgtg taaatcaatt
     3181 ttatcttttg ctatattttc agtaatatct gctaaaatcg aattagtaac tactaccttt
     3241 aatttttcgt tcgaagccac tgtatctttg tctttactat ttgttgaaca tgccccgaat
     3301 ataaaaagag caatcaaaat ccccagactt cccagtactg ctttccattt tctcatcata
     3361 actttctctc cttctttctt aaaaattttt gttttggtga gattagaaaa ctaattacaa
     3421 ataagaatgc cgctgttaaa acaatacttg atcctgctgc tatgttaaaa ctataaccaa
     3481 tgaacaaacc tagcaatgat gagcacgctc cgactgtcgc cgaaataaat atcatagttt
     3541 ttaaactgtt tgcatataaa tatgctgtag cagctggtgt aattaacatt gcaacaatta
     3601 atatagttcc cacactttgc atagctgtta ctgctactaa agttaataga atcattaata
     3661 gataatgata aaaactgaca ttcatcccca ttgcttttgc cattaatgga tcaaaagaag
     3721 tgattaataa ttctttaaaa aatagaacaa ctgtaaccaa cactaaaaca gaaacgccaa
     3781 ttgtaatcca cttatcaata tcttgtacag ctaaaatatt tccaaataag atatgaaata
     3841 aatccgttga gctatttgca actcctataa ggataactcc taatgctaaa aaagaactga
     3901 aagtaatacc aattgctgta tctcctttta ttacactatt gctttttata aaagtaataa
     3961 tgatagaagc taatagacca aatacaatcg caccaataaa aaaattaata cctaaaatat
     4021 aagaaattgc tactcctggc aaaacagcat gagaaatagc atcacccata agagacattc
     4081 ctcttaaaat aataaaacaa cctactgttc cagaaacaat ccctataact acagatgtaa
     4141 ttagagcatt ctgtaaaaag tgaaagtcat acagaccatt aataaaattt tgaagcatat
     4201 tattcccctt gtatattaga gttagggttt taaccttccg gttttgattg gataacccta
     4261 tgaaccatcg ttattttaat ttggtattaa ctcatttctt ttgagataat atatttattg
     4321 gattgagagg ctaagccgtt cccccctctc caataaatat agtatctcca taggcttttt
     4381 ttaaattctc ttcattaaat acagattcta cacttccgtg agcaattaga tttcgattca
     4441 aaagaataat attatcaaaa tacttcttta ccttgcttaa atcatgatgc acaattagca
     4501 ctgtttttcc ctgatgtttc aattctttta acgtatccat aattattcgt tcactaacta
     4561 agtctatccc tacaaatggt tcatctaaaa atatgaaatc cgcattttga gcaagacatc
     4621 ttgctaaaag tacacgttga aattgtcctc cagataattc tcctatttga tgattagaat
     4681 attttcccat atcgaccttt tccaaagcct ttgaaactct ttgccattct gcatttttaa
     4741 ttctttgaaa aactttcatt ccggcatatg ttcccataga tacacattct tttactttta
     4801 ttggaaaagt aaaatcaata tcagcttttt gctctacata tgcaatcttc accagttttt
     4861 tattgatatc ttcattatta atggagacct ctccctgatg agggataata tttaacattg
     4921 ctttaagcaa tgtagatttt ccagcaccat ttggaccaat aatacctgtt aaagcacctt
     4981 cttgtatttt taaagaaatc tcgttcaatg ctaaaaaatc gttataacaa acattaagct
     5041 ttttcacttc taacataact ttctccttct aaatgataat gattatcacg atcatatggt
     5101 atacaaaaat tatattttag tcaaccctaa attttataat ttagattttt ttctgttgtt
     5161 gaagattagg atgtcaaaat tatttgaaac tactcgtata gtaaatagta agtaattttc
     5221 aaaacacgaa gaaaaacaaa aagaaaaaag gaaacgccga agcatttcct ttcagtgatt
     5281 taatgagaac aacacgattt gacttagagt ctcaaaaagt cgaataggta cactcattta
     5341 agtgtctcca ttcgactttt tggggatgag ctggctattg aggtccgctt accctattga
     5401 ataaatacaa gattgggtat ttctaataag tccgcccttc atattggggt gatcggggcg
     5461 aacatgccat cttttcatat accacctcct cgacactcaa taatcaaaga ttctcgttaa
     5521 tcttcttcga tgcttgcaat gatgttttca atattcatat aacgaaaatc ttcttgtttg
     5581 atataaacga tctgagcggt acatttttcc tttttgatga tttcttttac tttttcctca
     5641 gaacgtgcga cctgcggcgt taaaagtaaa acgtcatagt ctttataatt cagtttttct
     5701 tcaacgtttc ggatcgttgc ataatctatc tcatacgata gatttttggc tctgatcgtt
     5761 tcaagcattt ttgatacgag cagcgacgca ctgattccgg tctcgcaaat aatcaataga
     5821 tccttatgca taataccctc ctgtttttag cctgcaaagc caattggtga aaatgccaca
     5881 taagttaaaa taacaatgac taccaatgct atagcagtca ccttggtgta tttccaaggc
     5941 tcgatatcga cctttgtatc gaatgagttc aacacaaatt ctccatcagg tttcaatttg
     6001 gaaaccacca tcaaaatgat aatgtccaga acgaataatg ccgtcaaaac ataaaggaaa
     6061 tggatatttt ggaaaagcat ttttgatagt gcatacaaga cgatatgcac ggcaatcgtc
     6121 gtttttgccc caaaagcagt tgcttttttt gaataaaagg caaagagaat gatcacgagc
     6181 agcggcatgt tgtatagtcc attgaattct tgaacgaact gatacaatcc agccggtgca
     6241 aaagaaatga atggcgctac gataatggag ataatcccca caacgaccgt tactgtttta
     6301 ccagcacgag cgatttgctt atcatttgct ttcttattga taagcggttt ataaaaatcc
     6361 agtgaaaaca aggtcgatgt cgcgttcaaa gctcccgcaa atgatgataa gatcgcgcca
     6421 aagataacag cggcgaaaat accaaagatc attttaggca atacttcact gactaaaaaa
     6481 ggatacgcgt tatctggatt tgacattaaa ctatcgccaa aaatattgcg agcgacgatc
     6541 cctggaaaga caaggaataa cgcacctaag attttgaaga cgccgactag gaaagcgccc
     6601 ttttgtgcct cagctaaatt cttgccggac aatgcttttt ggacgatcat ttgattggta
     6661 caccagaaat acagattgtt aaacagcatc cctgtaaata gtgtcggcca tggaacaaca
     6721 gccgagtcga tcgatcccac agagttaagc agccatggcg tgtttaccac aacgtgttcg
     6781 atcccgccaa caaagctgcc gtcaccaagc ttcattaagc ctaaaattgg gaccagcaga
     6841 ccacaagcca gcagaccgac tccataaatg gagtcactat tggcacttaa cgacaagccg
     6901 ccgatcagca gatagaggat gccgacaata ccaattgcca tcgctacaat tatgatcgcg
     6961 acgatcggct ggacgcccag cagctcatca atatggaaga tcttattgaa caccagcgat
     7021 ccagaataaa gaaccacggg taagaaagaa gtcatatagg tcacaataaa taagatcgaa
     7081 ataattcgtt tggtaaccgt atcataacga atctcgatga aatctgagat tgtattgatg
     7141 ccgtatttaa aatattttgg caagaatatc acagccaaag ctacgatcgc aatcgtagca
     7201 gttacttccc aggccattac ttccatccct gcatgataac tttgaccatt ttgtccgact
     7261 atttgttcgg tggacaggtt cgtcatgatg atcgtgccgg cgattggcaa agcagttaag
     7321 ctccgccctc ccatgaagaa cccttctgat ccagaagtat cgacacttct ggatctagaa
     7381 taggcaaata accatacaca gatgacaatc actaggaaac tgattataga aaaaagattc
     7441 atcgtctagc tccttttcaa tagattaaag accggtgacc tctcgaatgt agcttctggc
     7501 tttgactgca tattcaaatg gattggcttt agccgggtcc tgttctgctt caactacgat
     7561 ccagccttca tagtcgctgg catggattgc tgtccagatt ggagtgaaat caatcatccc
     7621 atcaccagga accgtgaaga cgcctttctt cacgccgttt aagaagctgt ctttttcctt
     7681 ttctacttct tcagccgttt cttgacgaat atctttgaaa tggatgtgtt tgatacgatc
     7741 ttgatgacgg ttgtaaaccg cgattggatt ttcaccagaa aagaccaaat gtcccgtatc
     7801 gaacaataat ttaactttgt ctggatcagt catttccatt aaacgatcga tttcttccgt
     7861 cgtttgaacg ccggtaccca tgtgatgatg gtaaacgatc tccatatcct tttcgtgggc
     7921 caaatcccct aatttttcta atccttccgc cagttttttc cactcttcat cagtgaaaac
     7981 tggtttatca tcaaagactg gcgctgtcat aatcccttgg atactatgac cctgctcagc
     8041 tactacgatg actttagcgc ccatcgcatg taagaaatcc cgatgtgctt tgaaggcttc
     8101 ggcagtttct tcaaaaggtt tagttgtcag aaacgcactg aaccatgcac tggcaacctt
     8161 taagccgcga atattcaaat attgttccaa cacctctgga tcttttggat atttgttgcc
     8221 aatctctgtt ccggtgaacc cagcaagggc catttcactg atacattgtt caaaggtatt
     8281 ttcactacca agctcgggca tatcatcatt ggtccacgcg attggtgcga tggctaattg
     8341 aatttgtttg gtcatattct cacactcctg cttatttttt tagttgtact aaactcttcg
     8401 tatcgaatga ttcttgacaa gccaacgcga tcttagttga ttgcaatcca tcttctgcat
     8461 taaccgctgg ttgacgtttt tcttgaatac atgagacaaa ttcttgggct tcacgaatga
     8521 aggcttctct gaatcgttct gggaagtttt gtgaacatgg acggatgatc ccttgatcgt
     8581 tcatgatcgt cacaagattt ttctcaggcg cttgcgctac ccgcagcatt cctttggttc
     8641 ccatgatctc tgtttcgaca tgatagccat gggctgcgtt tctgccggca actagtaaag
     8701 ctattgtttt atcgctcagg ctcatcattg cggctccagt ttcaagctca ccagcctgat
     8761 ccaattctgg atacgccgca tttttcccaa tcgcccacac ttgatcgact tcttggccgg
     8821 taaaccagcg aaccaaatca atatcatgga tcgccatatc taagaacaag ccgccgctgt
     8881 cgttattcat ggcaaatttg acaaagcttt ccaagccttc gctcggatcg atcccataac
     8941 aacgaatcag tgtgatttcg ccgatttcgc catcttctac cattttcttc gcatactgat
     9001 acgactcatc atagcggcgc atgaagccta acatgaagac ctgttcagga tgctcttcga
     9061 tgactttttg ggtcttttta atatcttcaa cttcaaggcc taatggtttt tcagtaaaga
     9121 catgcttgcc attttccatc gccaagcgga cttggtcaca gtgaaagcct gatggtgaaa
     9181 caataaagac tgcatcgatc acggaacttt tgaccatttc ttcatagctg gaaaaagtat
     9241 gcttgacgcc taatttcttc tgtgcgaatg ctaattctgt ttcaactaaa ctgcacgcag
     9301 ccgtaagctc acaacaactg acacctgttg ctaaattttg tgcatgtact tttcctaatc
     9361 ttcctaaacc gacgatgcct actctaatgt tgttcatgaa atacctccgt tttctgagaa
     9421 ttactcattg tatagtgctg gtcgttcatc catttgaatc attacttttg tttttgtttc
     9481 acgagactca tgacaagcca tcatggtgat cgctgctaca tacccatccc aagcggtagg
     9541 accgacaacc gcatcatttt tgatcgaatc gatccacagc ttgaattcat aatcataggc
     9601 atccaaaaag cgggtctgcc aatctggtga aacaggtgtg taattttttt cttccgattt
     9661 aattcgtgta taggctgggt ccgtcattcc tacttctgcc gtttcgaaaa ccacatcaca
     9721 tttaatatcg tagccaaagc ggcaattcac aaagatttcc aaatcaatcg ttacaccgga
     9781 ttttgtttga aagatcagca gctgcggatc atgcaaatcg gcatgggagt tgctggtttt
     9841 tttcggcaag atcatttgaa cagattcata atcttcattt agcaaccagc gaagcacatc
     9901 aatctcatga atcgcggtgt tgctgatcgc catcggtgtt gtgtagtttt catctgcgct
     9961 aggatttcta tgggcacaat gcatcattaa tgcttctccg tattgcttcg attcgattgc
    10021 ttctttcagt tcaatgtagc cgcggtcata gcggcgcatg aagccaacct gaaccaattt
    10081 cttcccataa gcaacttccg catcaacgat ccggcggcag cctgctgcat ccatcgctaa
    10141 tggcttttcg cagaaaacat atttcccatg cttgattgct tctaacacat actgctcatg
    10201 ggtcgggtcc caagaagtca caacaacgac atccacatct tcagaagcga tcaatgcttc
    10261 tccggtttca aagaatttag catcgacacg tttggcaacc tctttagcag cttttacatt
    10321 gatatctgag actgctgtca cataagcccc ttgtgttttc tgattgatcc tgtcgatatg
    10381 ctcgatgccg attgcacctg ttccgataac gcctactttt actctcaatt acttatcccc
    10441 ctgttctgtt gtttaagcac acatagtatg cgcttacaaa attattcctg tgtagctttt
    10501 attcttaaaa ggtagagccc tctctttgct ccgcctttta ctacccataa gcttgtacgt
    10561 ataattcctt aatttgacta tgactaggca cctttttatt attttgcgga cttccgctgg
    10621 tcaacgcatc cgcggccatt ttgtctaagg ctgccataaa cgcttgctga tcaataccat
    10681 aatctcgaat cgttgggatc gacagctgct cacagagctc ttggattttt ttaatcagat
    10741 aatccgcagc ctcttccagc gataaatctt cggctgccgg ataaataaat ctggcgatcg
    10801 tcgctaactc agtctgaata gcatcctttg agaattcaaa aaagaccggc aataacatcg
    10861 catttgatat cccatgagga atgtgaaaaa gtgcccccac cggtcgactc attccatgaa
    10921 tcaatgtgac tgacgcattc gaaaaggcaa tccccgcctc cgttgaagct aagctcattt
    10981 gttctctggc ttcagtgtcg tcgctatttt catatgcctg agaaagcgtg ttcaaaattt
    11041 tcttcatcgc ttccagtgaa aatagttgcg ttacttcatt ggctttgact gaaacataag
    11101 attccaaagc gtgacatagc gcatcgactc ccgttgccgc ggttactttt ggtggtacag
    11161 ttttcgtaaa atcagagtca acgattgaga tctgtggcat gaaaacgaga tccttcatca
    11221 tcattttgac tgctgttttt gtattggtaa taactgccac atcggttact tccgaaccag
    11281 ttccagcagt tgttgacact gcaatcagcg gaatgcccgc ttgcagctga tcaaaatttc
    11341 ttgcatgaat cgcttcgaga cttaattgat tagcagccaa aacggctacc accttggctg
    11401 tatccagaca gctgccgcca ccaaaagcaa tgatatgatc acattgatgc ttctgaaaaa
    11461 gcttcagcgc atcataaaca ttttcttgcg tcggttcggc ttgaatgtct gtatactccg
    11521 ctaattgatc atctgttttt cccaacagct ccttgatctt ttgaagatgc ccggtctgtt
    11581 ccataaccgt atcactaatg attaacggct tgttgcctag ttgttctgat tcttccataa
    11641 gaaattggat tgttccgccg ccaattctta aattggcagg cattttcagt tgaatattca
    11701 cagaaaaaac ctctctctgg cttatagcct ataacagaat cctcgaagct ctttaacaga
    11761 gcttcgaagc aaatctttaa atctcacttt gcgcataacg tgctgtaaca actttccgat
    11821 gggtataaaa ctcaacactg tctttgccat tggcatgcaa atcaccaaag aaagagtgct
    11881 tccagcctga aaaggcaaac caagccatcg gtgcaggcac cccaagattg acccccagca
    11941 tcccagcatc aacattttct ctgaagtagc gaatcgctga tgcgttgttg gtatacagac
    12001 acgaaccatt cgcaaattca gattcattcg tcagctcaac ggctgccatc aaatctttta
    12061 cccgcacgat tgatgcgacc ggggcaaaaa tctcatcctg ccagatcttc atatccatcg
    12121 tgacattatc aaaaatcgtt ggtccaacaa aatagccttt ttctggaatg ttttcgcgac
    12181 catctagtac cagtttcgct ccttcatcga cccctttttg gatatagtca aaggtccgct
    12241 gctgtgcatc ttttcgaatc actggtccta aaaagacccc ttcgtccaag ccattgccga
    12301 tcttgatcgc ttttgccgca gccttaaatt tttcaacaaa ctgatccgcg atttcctctt
    12361 ccaaaacaat gacggaggct gccatgcagc gttcaccagc agaaccaaaa gctccgccaa
    12421 taaggtcctt gactgcccga tcaatattgg catctttcag cacaacaaca tgatttttcg
    12481 ctccagtcaa cgcttgtacc cgttttagat tttttgttcc ttcacggtaa acgtattcac
    12541 cgacattttt tgatcctaca aagctgatgc cttttaccag tggatcttgc agcaatgtgt
    12601 tgacaacatc gtgggcacca tagacaacat taaaaacacc gctaggaaag cctgcttttt
    12661 caaccagctc aacaattttc tccatcaaca atggtgtttt ttctgaaggc ttcagcacca
    12721 ccgtatttcc tgccgccaga gccatcggga agatccagaa gggcaccatc attggaaaat
    12781 taaacggtgt gatccctccg attactccga ttgggtaacg ataattcgtg atctcaatat
    12841 cagtcgccgc tgttgataaa gaatctccca tcattaaatt ggtgattccc gctgcgtgct
    12901 ccacattttc gattcctcgt tgaacttctc ccagtgactc ggacaatgat ttcccatttt
    12961 ccagcgtgat caaacgcgcc aattcacttt tatgatctgt taaaagctga tgcagcttga
    13021 acagataacg tccgcgaaca ggaacaggaa cttttttcca agtttgaaac gcagcatcgg
    13081 cagctttaac tgcttcggct gtctctgctt tgctagacaa cggtacattc gccagcgctt
    13141 cttctgtagc cggattcatt acttcttcaa actctgttga gcgactagcg acccaacgac
    13201 catcaatata atttcttaaa aatcttgtca ttta
//
LOCUS       DAYYKN010000076        13212 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000076 DAYYKN010000000
VERSION     DAYYKN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.076"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACHBB7_001849"
     CDS             147..1016
                     /locus_tag="ACHBB7_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9209797.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACHBB7_001850"
     CDS             1225..2250
                     /locus_tag="ACHBB7_001850"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9209798.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACHBB7_001851"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACHBB7_001851"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM9209799.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACHBB7_001852"
     CDS             3333..5036
                     /locus_tag="ACHBB7_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9209800.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACHBB7_001853"
     CDS             5038..7071
                     /locus_tag="ACHBB7_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM9209801.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACHBB7_001854"
     CDS             7082..7522
                     /locus_tag="ACHBB7_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9209802.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACHBB7_001855"
     CDS             complement(7722..8849)
                     /locus_tag="ACHBB7_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9209803.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACHBB7_001856"
     CDS             complement(8852..9529)
                     /locus_tag="ACHBB7_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9209804.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACHBB7_001857"
     CDS             9793..11559
                     /locus_tag="ACHBB7_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM9209805.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACHBB7_001858"
     CDS             11574..12011
                     /locus_tag="ACHBB7_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9209806.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACHBB7_001859"
     CDS             12024..13175
                     /locus_tag="ACHBB7_001859"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM9209807.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAYYKN010000077        13084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000077 DAYYKN010000000
VERSION     DAYYKN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.077"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..2198)
                     /locus_tag="ACHBB7_001860"
     CDS             complement(78..2198)
                     /locus_tag="ACHBB7_001860"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9209808.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACHBB7_001861"
     CDS             2428..3606
                     /locus_tag="ACHBB7_001861"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM9209809.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACHBB7_001862"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACHBB7_001862"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM9209810.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACHBB7_001863"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACHBB7_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM9209811.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACHBB7_001864"
     CDS             complement(4959..6212)
                     /locus_tag="ACHBB7_001864"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9209812.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACHBB7_001865"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACHBB7_001865"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM9209813.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACHBB7_001866"
     CDS             7789..9135
                     /locus_tag="ACHBB7_001866"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM9209814.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="ACHBB7_001867"
     CDS             complement(9443..9829)
                     /locus_tag="ACHBB7_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9209815.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10097..10246)
                     /locus_tag="ACHBB7_001868"
     CDS             complement(10097..10246)
                     /locus_tag="ACHBB7_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209816.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10708..10920
                     /locus_tag="ACHBB7_001869"
     CDS             10708..10920
                     /locus_tag="ACHBB7_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209817.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11198..12997)
                     /locus_tag="ACHBB7_001870"
     CDS             complement(11198..12997)
                     /locus_tag="ACHBB7_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM9209818.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3863 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtgttaaa aaatagttat ctaaaaagca gaaagatact caaaataaaa aataaaaaaa
    10621 tgaataaaaa cgttttcaaa tacaaggtaa tttgttatta taaaataaag aaaaaacaaa
    10681 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
    10741 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
    10801 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
    10861 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
    10921 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
    10981 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
    11041 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
    11101 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
    11161 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
    11221 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
    11281 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
    11341 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
    11401 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
    11461 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
    11521 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
    11581 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
    11641 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca aaagaacaaa
    11701 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
    11761 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
    11821 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
    11881 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
    11941 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
    12001 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
    12061 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
    12121 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
    12181 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
    12241 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
    12301 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
    12361 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
    12421 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
    12481 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
    12541 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
    12601 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
    12661 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
    12721 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
    12781 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
    12841 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
    12901 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
    12961 cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct cttcacattg
    13021 tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat
    13081 ttaa
//
LOCUS       DAYYKN010000078        12942 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000078 DAYYKN010000000
VERSION     DAYYKN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.078"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..572
                     /locus_tag="ACHBB7_001871"
     CDS             135..572
                     /locus_tag="ACHBB7_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM9209819.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            648..1268
                     /gene="rpoE"
                     /locus_tag="ACHBB7_001872"
     CDS             648..1268
                     /gene="rpoE"
                     /locus_tag="ACHBB7_001872"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM9209820.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            1457..1963
                     /locus_tag="ACHBB7_001873"
     CDS             1457..1963
                     /locus_tag="ACHBB7_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM9209821.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            1980..2879
                     /locus_tag="ACHBB7_001874"
     CDS             1980..2879
                     /locus_tag="ACHBB7_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM9209822.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            2966..3850
                     /locus_tag="ACHBB7_001875"
     CDS             2966..3850
                     /locus_tag="ACHBB7_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM9209823.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(4042..4113)
                     /locus_tag="ACHBB7_001876"
     tRNA            complement(4042..4113)
                     /locus_tag="ACHBB7_001876"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4078..4080),aa:Arg,seq:ccg)
     gene            4173..5507
                     /gene="rpoN"
                     /locus_tag="ACHBB7_001877"
     CDS             4173..5507
                     /gene="rpoN"
                     /locus_tag="ACHBB7_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM9209824.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            5656..6747
                     /locus_tag="ACHBB7_001878"
     CDS             5656..6747
                     /locus_tag="ACHBB7_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM9209825.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            6788..7789
                     /gene="gap"
                     /locus_tag="ACHBB7_001879"
     CDS             6788..7789
                     /gene="gap"
                     /locus_tag="ACHBB7_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM9209826.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            7899..9089
                     /gene="pgk"
                     /locus_tag="ACHBB7_001880"
     CDS             7899..9089
                     /gene="pgk"
                     /locus_tag="ACHBB7_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM9209827.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            9193..9948
                     /gene="tpiA"
                     /locus_tag="ACHBB7_001881"
     CDS             9193..9948
                     /gene="tpiA"
                     /locus_tag="ACHBB7_001881"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM9209828.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            10064..11362
                     /gene="eno"
                     /locus_tag="ACHBB7_001882"
     CDS             10064..11362
                     /gene="eno"
                     /locus_tag="ACHBB7_001882"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM9209829.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            11761..12900
                     /locus_tag="ACHBB7_001883"
     CDS             11761..12900
                     /locus_tag="ACHBB7_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM9209830.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4526 a         2336 c         2733 g         3347 t
ORIGIN      
        1 ttttctacaa tattatactc taaaatcaaa gagttgtttt ggaaaaaagg ttttctgtaa
       61 aaatggacaa gttggttgac aaaacaaaag aaactaggaa caatgtagct atgagaagta
      121 aaggagaatg aataatggaa ttatcacaag gaacaccgat ttcaatcaaa ttgcgtacaa
      181 aagtaaaaca gcaaggggaa gtgcaagatt tttattttga tttgaaaggg caaatggtga
      241 caattggcga tacgctgtac atacgttata aagagattca ggaggagacg ggcgatgaga
      301 ttcctgtaac gatcaaatta gttccagatg gtcatgtaca gctgatccgt gcaggagaaa
      361 tgcggatgcg tttgaaattt ggctataaag aacggttaga aacatcctat cgaacaccat
      421 acggtatgat ccagattgct acttttacga aagaactgca tgttagtttg aaagaccgtc
      481 ccacagcagg gaaggttcga atcgattatg atctgttcat gggaccggaa aaaatcggcg
      541 aatattattt gaccttggat tttacagcgt aaatataaaa gaaaagtttg attttttgtg
      601 tctgattttg tatgataagg agtagactgt gaaaggacgt gtcctgattg gaaattagtg
      661 tatttgaagg tgcaaacaaa agcgaattat caatgatcga agtagcacac gcgatcttag
      721 aacaacgtgg cgatgtcatg gatttttctg atttagctaa ccagatccaa aactatcttg
      781 ggaaatcaga cagtgaaatt cgtgattcac tcgcacaatt ttatactgac ttgaatattg
      841 acggcagctt tatctcatta ggggataatc gttggggctt gcgttcatgg tatgctatcg
      901 attcgatcga tgaagaagta aaccatggaa tggacgaaga ggatgaagat actccacgtc
      961 gccgtaaacg caagaaagtc aacgccttta tcaatgatga tgaagacgcg atcgattaca
     1021 atgacgatga cccagaagat acagatctga cagaagaaga tgacgatgat ctgtttgatg
     1081 acgatgatga tgaagatgaa gaaatcgctg cttataattc tgacttgcaa gaaatcggag
     1141 cagatgaccc gacagatgat gaagaagact tgccaggaaa tatcgaagaa gatttaagta
     1201 tcatagacga agacgatgat gatgaagatt atgaagagga agaattttct gactctgaaa
     1261 aagaataaac attattttct tacgagaggt tgtgacagag acgatcagcc tatcagaata
     1321 aggaggtgtg acacgatttt tgtcacacct tcttttttgt atataaaaat cccaagtaaa
     1381 tacataaaaa ataaaaaaat tgaaactttt agcgcacctt gtacgtcata ttaagtgagg
     1441 cagaaaggag tggagaatga tttttactgg atataagaaa aaacggcaga tcgaaaaata
     1501 ccgtcacctg ctgaatggac tcatcgaaac tgattatcaa aaaatgttcc gtatcacgat
     1561 gaattacgta catgataaag aagaggcctt agatgtgatg caagatagtt ttcataaagc
     1621 cctgaactat tttgaaaaag aaaaagagat cgagcatttt agtgcatggt tttatcggat
     1681 attgatccgc acggcattag atagttggcg cagacaaaaa aggacgcctg tggacttatt
     1741 tgaaattcaa gatatccccc aacagttacc tttagaaact tccgttgcag agctccatat
     1801 gattttagat aaaatagaca gtccggaaaa agagattctg atcctctact tttttgaagg
     1861 atttcgtttg aaagaaattg cgactatctt ggatatgaat gaaaacacag ttaaaacgaa
     1921 aatgtaccgc tcattgaatg ctttacgtca aatcctagaa tgaaggagag aagagtgcca
     1981 tgtattccca aaaagaaaat ttaaaaaaac taaagaaaac ttataaagaa caagcaattc
     2041 ccaaagggct taaagaggag atccgccagc gttttattat cgaagagcaa tcatttataa
     2101 aagcaaagcg gagaaagaga cgtctccaag ggttcggagt atctttggct atcgtaatga
     2161 ttacaacaag tagccttctt tttaatagtc aagttcgaag ctttgcagaa gatttaccca
     2221 tcttaggatc agtcattgaa ttgatcttag gcgaacgctt tactgatcga tctgaaaaaa
     2281 tcgatattca ggtaccgaag atcaatacac aaaatgagaa agaaaataaa acaattcatg
     2341 gattaaatca aaaatacttt cgcgaaggac aagcagattt tgaaaaagct gaaaaagaat
     2401 atggaaattt tgaaacggat cattaccaag ttttgggaga ttatcagaaa atcgtagacg
     2461 ataaccggtt tctcgtgatt gaaaggcaga tcacccaaac agcagcagat agccatgttg
     2521 aaaaaagata cgacacaatc gacaagaaaa acagtgtcca gctctcactt ccacttttat
     2581 tcaaagatga cacctatcta tctgttttga caaaagaagt caaacggcag atggccgaac
     2641 aagtcaagga agatccatct aagtactatt ggactgagca agatattcaa gaagggacga
     2701 tagaaaaacc aacacttgtt acgcctacac gaagttttta tttgaacaaa gagcatcaat
     2761 tagttttgac tttttctcaa tacgaaattg ctcctggtta tatgggtaca ccagaatttg
     2821 tcattcctaa gtccgtgaca aaaacgattt tggcatcgga agattatttg gatcattaga
     2881 cgagaagtag ttgcgactag cagtttcaat tttcaagtaa gtacaagtaa ataataagaa
     2941 gagaaaaaga gaaagaagag atatagtgaa aagaaattat ttagttatca gtattattgc
     3001 tttatccgtg cttttgtttt tcgaaattac ttcggtggcc tccttgatca ccgaagtaaa
     3061 aggacaaaca cactcttcgg agcattcgtc acaatctttc aaacaatcag aacaatcaac
     3121 cattaaaagc caatcaactg aaacagctaa cgtcaaagaa gccattcaag aatcagcgga
     3181 aaaggtacaa agtcagtcat cacaatcaac acaagacagt acaaaaacga gccgagatcc
     3241 ggctgtcaaa agcgtcgcaa tcagctttga tgatggacca ggagcgacca caacaccgca
     3301 gcttctgcga atcttaaaag aaaaaaacgt gcatgccact tttttcgtct taggagaaaa
     3361 tacggcgcag catcctgaaa tcgtgaagca gacagctgaa gcagggcacg agattggcaa
     3421 tcacacatac gatcatcaga atctggccac tctttctgcc cagtcgatga cggaggaagt
     3481 gacgaaagca gacactgaga tcaaaaaagt cactggcaaa acccccactt ttgttcgtcc
     3541 gccctatggt tctgtcacga atgtaggagc aacgatcatc cagcgaccga tcatcgaatg
     3601 gtctgttgat tcagaagatt ggaagacaag aaatcctgat ttgattttgc agaagatcca
     3661 agcaacagta tacgatgggg caattatttt attccatgat atctatcctg aaacgattcg
     3721 tgccgttcct caagtgatcg attatctgaa agagcaaggg tatcgcatca cgacagttgg
     3781 ggatttatta gggcatccaa cggctgtcga gaactattat ggcagaaacg atcatcggcc
     3841 agttcagtaa gtgagacaag tatctttaat aaataacaca ttttaataag tagttgaaca
     3901 cataactacc ttacttgcaa aggcttaaca atatctatac aggtacaaca aaaaactgtc
     3961 caccaatttt ctttggcgga cagttttcac ttaaaaataa agaagcaact attcgtaaat
     4021 tatgctaaaa tagcagtaga acgcgtcctg agggaatcga acccccgtct caagaaccgg
     4081 aatcttacgt gatatccact acactaagga cgcaagcact gggataattg taactaaaca
     4141 gataaaaaat tacaagggga ttggacaaaa aaatgaaatt tgagcaaaat ttttcacaaa
     4201 aacagcagca gacacagaaa ctagccatga cgcaacagtt gcaacagtcc atccaagttt
     4261 tacaatataa cagtgaggag ctgttggcat tcgttgagaa tcaagcaatg gaaaatccat
     4321 tggtggaagt tgttgagccg gaatggcagc cagattacat aaaagcttct tcatcttctt
     4381 atgaaggaga agagacaaat tacctaaatc agattcctga tacaaaaggg tctttatttg
     4441 attcattgat cgaacaagtc catttgaatt accgagatac ttttttgagg aaaatcgttc
     4501 tttatcttgt agaatacatc gatttgaacg gatttttgac gattagttta gaagaagccg
     4561 gaaaagagat tggagctacg cctatccaaa tgctcgatgc gttgactctg attcagcaat
     4621 tagaaccagc tggagtaggt gcacgtagtt tgcaagaatg tctcatgctt caaacagaac
     4681 gagatgatta tgcgccggaa ttagcttata tcgtgttgga agaatgtttt gaggaactgg
     4741 tggagcgaaa atggaaagaa atcgcacaac gtttttctgt tgatctacat gctgttcagc
     4801 agatattcga ctatttacaa acgttgagtc caagccctgg ccggattttc gaccgtagca
     4861 gcgaattgta cattattcct gatgtccggg tattagtgga cgacgataaa aatgtacaag
     4921 ttatctcaaa ccgaaaaaat cagccaaata tacgctttca agagagttac ttcaaacaaa
     4981 tgtcacaaca agcagataaa gaaactgaaa attatttaaa agaacggaaa caggaattcg
     5041 aatggttgaa gaaaacgatc ctacaacgtg gggatacaat tttgcgggtt gctcaagtca
     5101 ttgtttcccg gcaaaaagct ttctttatcg acaaggaacg tccaatcaag cctcttacgt
     5161 taaaggaagt tgcaactgag atagatgtgc atgaatccac ggtcagtcgt gcagtcaacg
     5221 ggaaatattt ggaaacagac ttcggtgtat ttgaattaaa aaaatttttc actacacgaa
     5281 ttcctacgaa tagtacagaa cagacagaag acctttcagc agatacagcg aagaaaaagc
     5341 tgcaggaatt agtcgatcaa gaagacaaaa acaagccgtt gtctgaccag aaattggttg
     5401 aattgctgaa aaaagatgag atagcgatct caagacgaac agtagcaaaa tatcgtgatt
     5461 tattaggaat cccgagttca tcaaaaagaa aacgttatga taactaatca gtcaaaagac
     5521 tagaaaaagt atttcacttt ttctagtctt tttagttgaa aaacgagagc aaaactgtta
     5581 gacttacagt cgtgaggttg agatttccct ttattgatgt gaaattcgaa tagttaattt
     5641 ctttttttat cgtatatggg tcgttttaag tctacattgg acgtaaagtg tccaactaag
     5701 gagatcgcta tgctggatga aataaaaatg attgaatctg ttgcaccaga tattatcgaa
     5761 gtgttgcaag aacgctataa aattttacga aatatctatt ggatgcagcc gattggacgt
     5821 cgaagtttat cggaaagtat ggggatcaca gaacgagtat tgcgaacaga aacagatctt
     5881 ttgaaaaatc tgaatttgat cgacacctca aaaagcggta tgacgctgac aactaagggc
     5941 gaagaagttt atcaaagcct tgaaaacttc atggatcagc ttttaggtac gcatcaaacc
     6001 gaacaacagt tggcagaata ttttggtaac caacgctgta tcgttgtttc aggaaacagc
     6061 gaagagcaga ccaaagtggc tgatgcattc ggcgaagcgc tgagcgaagc cctagaccga
     6121 ctacttccag aaggcgagaa tatcatcgct gtgatgggag gaactactat ggctgtggta
     6181 gccgaacaat tgtctaatct ggaaaataag aaacggcata acctgtttgt ccctgcaaga
     6241 ggcgggattg gtgaagcgat aactgtacag gcaaattctg tcagtgcaag aatggctgct
     6301 aaagcaaacg gtaaccaccg tgcactttat gttccggaac aattaagtct ggcaacttac
     6361 aattctttac tcaatgagcc atctatccaa gaagtcttaa acttgattag tgaagcgaat
     6421 tgtgttattc atagtattgg gcgtgcatta cacatggcag cacgtcgtaa gatgactgaa
     6481 aaagaattag tcatgctgaa acaagcaaat gctgttgcgg aatcttttgg ctactttttc
     6541 aatgaaaaag gtgaagtcgt ttataaggtc cctagaatcg gcatagaatt gcaagatttg
     6601 gaaaaagtcc cgatcatcat ggcaatcgcg ggcggaaaat caaaagctaa agcaattcga
     6661 gcttatatga aaaatgcacc aaaacaaacg tggctcatca ctgatgaagc tgctgcaaat
     6721 gagattttaa aaggggtaac cctttaaaat aaatattttt ttgattttca taaggaggaa
     6781 atcttaaatg acagttaaag taggtattaa tggttttgga cgtattggac gtctagcatt
     6841 ccgtcgtatc caagatgtag atggaatcga agtagtagca atcaacgact tgacagatgc
     6901 taaaatgttg gcacacttgt taaaatacga cacaactcaa ggacgtttca acggaacagt
     6961 tgaagttcat gaaggttcat tcaacgtaaa cggaaaagaa gttaaagtat tagctaaccg
     7021 caacccagaa gaattaccat ggggcgaact aggcgtagat atcgttctag aatgtactgg
     7081 tttcttcact tctaaatcag cagctgaaaa acatttaaca gctggtgcta aacgcgttgt
     7141 tatctctgct cctggcggaa acgatgttcc aacaatcgtt tataacacaa accacgaaac
     7201 attaactggt aaagaaacag ttatctcagg tgcttcatgt actactaact gtttagctcc
     7261 tatggctaaa acattgaacg acaaatttgg tgttgttgaa ggattaatga caactatcca
     7321 cgcttacaca ggtgaccaaa tgactctaga cggacctcat cctaaaggtg acttccgccg
     7381 tgcacgcgct gctgctgcaa acatcgttcc taactcaaca ggtgctgcta aagctatcgg
     7441 tttagtaatt cctgaattga acggtaaatt agacggagct gctcaacgtg ttcctgtacc
     7501 aacaggttca ttaacagaat tagtaacagt tcttgaaaaa gaagtaactg ttgacgaaat
     7561 caatgcagca atgaaagaag cttcaaacga atcttacgga tacaacacag acgaaatcgt
     7621 ttcttcagat atcgttggta tgacttacgg ttcattattt gatgctacac aaactaaagt
     7681 aatgacagtt ggcgacaaac aattagttaa aactgttgct tggtacgaca acgaaatgtc
     7741 atacactgca caattagtac gtactttaga atacttcgct aacttataag atagacaaac
     7801 gtacaagtgt aaattataga catgtacatt taaaagcggg gaagcaatcg cgcttcttcc
     7861 gctttttttg ataataagtt tattcaggag gaaataaaat ggctaagaaa acagtgaaag
     7921 atatcgattt aaaagacaaa aaagttcttg tccgtgttga cttcaacgtt cctttgaaag
     7981 acggcgtgat cactgacgac actcgtatca aagcagcatt gccaacaatc aactacgttc
     8041 tagaacaagg cggaaaagca atccttttct ctcaccttgg acgtgtaaaa acagaagaag
     8101 ataaagaagg aaaatcttta gctccagttg caaaacgtct aggcgaatta ttaggtaaag
     8161 aagtaacatt cgtacctgaa actcgcggag aacaattaga agaagcaatc cgcaacatga
     8221 atgacggcga cgttgtcgta ttcgaaaata cacgatttga agatattgac ggtaaaaaag
     8281 aaagcaaaaa tgatacagaa ctaggtaaat actgggcttc attaggcgat gtatttgtta
     8341 acgatgcttt cggtacagct caccgtgcac atgcttctaa cgtaggtatc gcttcaactg
     8401 gtatcccaac agttgctgga ttcttgatgg aaaaagaaat caagttcatc ggtgaagcag
     8461 tagaagaacc aaaacgacca atgattgcaa tccttggtgg cgcaaaagtt tctgataaaa
     8521 tcggcgtaat cgaaaacttg attcctaaag cagataaaat cttgatcggt ggcggaatga
     8581 cttacacatt ctacgaagca aaaggaatca agatcggtaa ctctctagta gaagctgata
     8641 aagtggaact agcaaaagaa ttaatcgaaa aagcaggcga caaacttgtc ttgccaatcg
     8701 acaatgtctg tgcaccagaa ttttcaaatg atgttgaaac acaagtaatc gaaggagaca
     8761 ttcctgatgg tttgatggct ttagacatcg gcccagcatc agtaaaatta tttgctgata
     8821 cattacaagg tgcaaaaaca gttgtttgga acggacctat gggtgtattt gaaatgagta
     8881 actttgctaa aggtacaatc ggtgtttgtg aagcaatcgc taacttagaa ggcgcaacaa
     8941 caatcatcgg tggcggagac tcagccgcag cagcagaaca attaggattt gctgacaaat
     9001 tcacacacat ctcaacaggt ggcggcgcaa gcttggaatt gcttgaaggt aaagaattac
     9061 caggactagc agcaatcaac gataaataaa cgaccaagtt tgctcaattc tgacgagtaa
     9121 tcgaacaaaa ggtgaaggga gttttcatga ctccctagcc atttttcaat aaattaagga
     9181 gtgtgttcct caatgcgtaa acctattatc gcaggtaact ggaaaatgaa caaaactgca
     9241 tcagaagcaa aagaatttgc tgaagcagta aaaaacaaaa tcccttcaaa tgacaaagtt
     9301 gattcagtca tcggatctcc tgcattattc ttacaagaat taatgacatc agctgaaggt
     9361 acagaactaa aaatctcagc acaaaactgc tactgggaaa actcaggtgc gttcactggt
     9421 gaaacttcac cagcagctct tgctgatcta ggtgttcatt atgtgatcat cggacattca
     9481 gaacgtcgtg aatatttcca tgaaacagac gaagacatca ataaaaaagc gaaagctatc
     9541 tttgcaaatg gtatgacacc aatcctatgc tgtggcgaat ctttagaaac ctatgaagca
     9601 ggcaaaactg ctgaatggat cgaaggacaa attacagctg gtttagctga tctttcagct
     9661 gaacaggtgt caaacatggt tattgcttat gaaccaatct gggcaatcgg tactggtaaa
     9721 tcagctgacg cacaaatcgc tgacgaaatt tgtggagttg tacgtcaaac agttgaaaaa
     9781 ctttatggaa aagaagtatc agaagcagtt cgtatccaat acggcggttc tgtaaaacca
     9841 gaaaatattg ctgaatacat ggcaaaagaa aatgttgacg gtgctttagt cggtggcgca
     9901 agcttgcaag cagattcatt cttgtcattg ttagacgctg taaaataatt gttgaaaatt
     9961 tgcgaaaaat gcaagaaatg tttgcactaa tgagcaaaat taactaaaat cgtatatgag
    10021 ggaaatccct taggaaaaaa caaactcaaa ggagagacaa aacatgtcaa ttattactga
    10081 tgtttacgct cgcgagatct tagactcacg tggtaaccca acaatcgaag tagaagtata
    10141 cacagaaagc ggagcttttg gccgcggaat ggttccttca ggtgcttcaa ctggtgaata
    10201 cgaagcagtt gaattacgtg acggagacaa agctcgttat ggcggtaaag gtgtaactaa
    10261 agctgtcgac aacgtaaaca acatcattgc tgaagcaatc attggctacg atgttcgtga
    10321 ccaaatggct atcgacaaag ctatgatcgc tttagacgga actcctaaca aaggtaaatt
    10381 aggtgcgaac gctattttag gtgtttctat cgctgttgca cgtgcagctg ctgactacct
    10441 agaagtacct ttataccact acctaggcgg attcaacaca aaagtattgc caacaccaat
    10501 gatgaacatc atcaacggcg gatcacatgc tgataactca atcgacttcc aagaattcat
    10561 gatcatgcct gttggcgctc caacattcaa agaagctcta cgtatgggtg ctgaagtatt
    10621 ccacgcatta gcttcaatct tgaaagctcg cggattagca acttctgttg gtgacgaagg
    10681 tggatttgct ccaaaccttg gttcaaacga agaaggtttt gaagtaatca tcgaagcaat
    10741 cgaaaaagct ggctatgtac ctggtaaaga cgttgttctt gctatggatg ctgcttcttc
    10801 agaattctac gacaaagaaa aaggtgttta cgtactagct gattcaggcg aaggcgaaaa
    10861 aacaactgac gaaatgatca aattctacga agaattagtt tctaaatatc caatcatctc
    10921 aatcgaagac ggattagacg aaaacgactg ggacggattc aagaaattaa ctgacgtatt
    10981 aggcgacaaa gttcaattag ttggtgacga cttgttcgta acaaacactc aaaaattatc
    11041 tgaaggtatc gaaaaaggaa ttgctaactc aatccttatc aaagtgaacc aaatcggtac
    11101 attaacagaa actttcgaag ctatcgaaat ggctaaagaa gctggctaca cagcagttgt
    11161 atctcaccgt tctggtgaaa cagaagattc aacaatctct gatatcgctg ttgcaacaaa
    11221 cgctggccaa atcaaaactg gttctctatc acgtactgac cgtattgcta aatacaacca
    11281 attactacgt atcgaagacc aacttggtga agttgcagaa tacaaaggtt tgaaatcttt
    11341 ctacaactta aaaaacaaat aattcttttc taatgaagat acatgatcga ctattgaaag
    11401 atagtcgagt atgatcactt agttaagtaa tcgaactctc tacttgctga aatatcagca
    11461 ggtagagagt ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga
    11521 aaagatcatc cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag
    11581 gatagaacag tagaatagga atctatgagc aagtcctctc gtataaataa aataggaggg
    11641 aagtagcagc atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc
    11701 tatttgaaaa ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag
    11761 atgaaaaaag aagaaaaaat cgcgatttta caagagatca tccgaattaa atcagtgaat
    11821 ggaaacgaag gtgaagtagc agcctatttg aataagcttt tggcaaagca tgacataaca
    11881 ggagagattg tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc
    11941 caatcaggaa aagtgttagg cctatctggg catatggatg tcgtggcggc tggtgatgaa
    12001 tcgtcttgga cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt
    12061 ggtgctacag atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa
    12121 gagtcaggaa aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg
    12181 ggtgaactag gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg
    12241 atcatcggag agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat
    12301 acagtcacct cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca
    12361 atcaatcacc taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa
    12421 acaatcgaga atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg
    12481 aatcaagtca acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca
    12541 gaatacccaa atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa
    12601 gagacagact atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat
    12661 ccagattctc ctttgattca ttgcattcag caacaattca gccagccatt acctttagtt
    12721 ggagcggcag caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt
    12781 gtcgtctttg ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc
    12841 gataattatt tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa
    12901 gattttttta ataaatgaaa aaacagagcc agattagcat ta
//
LOCUS       DAYYKN010000079        12815 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000079 DAYYKN010000000
VERSION     DAYYKN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.079"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..2336
                     /gene="acm"
                     /locus_tag="ACHBB7_001884"
     CDS             171..2336
                     /gene="acm"
                     /locus_tag="ACHBB7_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM9209831.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2608..3093)
                     /locus_tag="ACHBB7_001885"
     CDS             complement(2608..3093)
                     /locus_tag="ACHBB7_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM9209832.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3183..3528
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3573..4214
                     /locus_tag="ACHBB7_001886"
     CDS             3573..4214
                     /locus_tag="ACHBB7_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9209833.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4233..4868
                     /locus_tag="ACHBB7_001887"
     CDS             4233..4868
                     /locus_tag="ACHBB7_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9209834.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4886..5701
                     /locus_tag="ACHBB7_001888"
     CDS             4886..5701
                     /locus_tag="ACHBB7_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9209835.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6020..7456
                     /locus_tag="ACHBB7_001889"
     CDS             6020..7456
                     /locus_tag="ACHBB7_001889"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9209836.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7645..10092
                     /locus_tag="ACHBB7_001890"
     CDS             7645..10092
                     /locus_tag="ACHBB7_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM9209837.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10322..11389
                     /gene="rlmN"
                     /locus_tag="ACHBB7_001891"
     CDS             10322..11389
                     /gene="rlmN"
                     /locus_tag="ACHBB7_001891"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM9209838.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11379..11585
                     /locus_tag="ACHBB7_001892"
     CDS             11379..11585
                     /locus_tag="ACHBB7_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209839.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11582..12685)
                     /locus_tag="ACHBB7_001893"
     CDS             complement(11582..12685)
                     /locus_tag="ACHBB7_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9209840.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4372 a         2141 c         2735 g         3567 t
ORIGIN      
        1 ccttgtaaaa acagtttttt ggcatattta ttttttataa atatttgtaa aataactatc
       61 aattttttag ttaaaagatt ggaaatgagt gggggaggaa ttgaagaaag gatttttgag
      121 agatgatata gtaggtttta tttttagtta aaatagggga gatagaaaaa atgaaaaagt
      181 gtagaaatat tattttatca atgttattta ttattactaa tataacatca ctgattcctg
      241 ttcatgtcta tgcggatgca ggcagagata tcagcagtaa tgtcacttcg ttgaccgtgg
      301 acccaacgaa tattacagat ggaggaaaca taaaagtaaa attctcgttt gacgaaaaaa
      361 aacaaaatat ccaaccaggt gattatcttt ggatcaattg gccaagtgaa ggaaatattc
      421 gcggtgaagg attccaaaaa gaaatcccat taatgattga aaataaaaat gttggaactt
      481 taacggtcag aaaagattct gcgcaggttg tgtttaatga aaatattaaa aacctagact
      541 cagtagaagg ttgggggcaa tttgagatac aggccagaaa cgtaaccgat accggtgaag
      601 aaaatacggg accattcacc gtgacgagcg gtgataaaac agctactgta aatgtgacaa
      661 agccagcttc tggttcttct tctagcgttt tttattataa aacaggagac atgctacctg
      721 aagatactaa gcacattcga tggtttttga atattaacaa taacggcact tatgttgaac
      781 aaccagttaa aatcagtgat gaaatccaaa gtggacaaag attagatcca agtacgtttg
      841 aaattaatca aatacactta ggtgaacaga aggtttatag aggagaagag ggaatccaac
      901 aatttttaca agatttcccc agcgctacat ttaatttttc agtaactgat aattacattg
      961 aaataactat accaaaaaac tttgtcaatc ttaggaaaat aatggtgagt tataagacga
     1021 tcatagaaaa cccagaacaa ataaattttg agaatcattc ggaagcttgg tttaaagaat
     1081 ttaataaacc tgctgttgat ggagaaagtt tcaatcatac agttaaaaat atttcagctt
     1141 ctggtggagt caacggtaca gttagaggcg aattgaagat tttcaaatat atcaatgaca
     1201 cagagattgg tatcccaaat gtgactttcg aattaagacg agcagatgaa cagcctattc
     1261 aaggacagtc tagtatcctg ttgacttcga atgaacaggg agaagctagt atcaaaggcc
     1321 tgcaagtagg agactatgtt gtaaaagaaa aagaagcacc caattggatt gattttgatc
     1381 cactaagttc aaatgaactc aaattctcca taaacgaaaa tgatacagag ggagtcagtt
     1441 tacctattta taacaagaaa aaggttacga acatcacagc aactaaaatt tggaacggtg
     1501 gaaccactcc aagaccgagt atttatttta aattattcag agcaagtaca aacaattggg
     1561 aaccagttcc tgatgcagaa acaaaacgat tagacaatgg aataacttca gtgacttggg
     1621 aagatatcca acagtatgat gatagtggaa acgaatatac gtttaaagtg caagaagtag
     1681 atcaaaacgg aaatgattat gtcccttcag gttatcaaaa aattgaaaac gggctagtcg
     1741 ttacaaatga gaataaagaa gtcatttcag tctcaggaca aaaaacatgg gaagacaatg
     1801 agaaccaaga cggcaaacga ccgacgacga tcacagtaaa tctattagcc gacggtcaac
     1861 cgatccaaca caaagaagta agtgaaaaag atgattggcg ctatcgtttc acaaatctgc
     1921 caaaatacag agacgggcaa gaaatcatct atactgtaac ggaagacaac gtgcccgaat
     1981 acagtacgac gattgacggg tataacatca aaaacagtta tacaccggaa aaaacaaata
     2041 tatctatacc aggtagccat ataacaccgt ggaaaaattt tccagagaag acagagagag
     2101 agaaaattag aaatctattc tctcaaaaat tgcaaatatt tgctcataca ggagaatcca
     2161 gcgaacaaag aagcaattat attagtaaga gagctgtacc taaacagata acaaataaat
     2221 caaaaagcat tcttcctaaa acaagtagta aaaaatctag ttttgctgta atcattggtt
     2281 tattgatagt aactatctgt gctgggatat tcttacaaaa atataaaaaa gtttagttag
     2341 aatataatat tttttgttaa atatgactga aatcaaaaga ataaaattcc gatttataat
     2401 cgaaaaaaga agcattttta tatatgcttc ttttttcgta gaatcaataa atatggattt
     2461 ggctaactat ggaagaatac tcgtattagt tttcagcgtt catactagtt gttacattgt
     2521 ttgattggcc aaaagaattt ctgatatatt ttgtgctaaa taaagaaaaa gacgcttgaa
     2581 ttgggaaata agaaaactac tctatcatta caaattttca taaactggca gatgttctaa
     2641 gtaaacaagt atcttttcaa agtcggaaaa atccataatc aacgtagtgg tattgatatt
     2701 tggatgaaag cctacggtat cttgacgatc aatagcagca tcgatcacga cttggatttt
     2761 tttctctttg tcgtgcatca atgctaatgg agtcactgta cctgctggaa cgcctaggag
     2821 gtcaggaagc tgttcttcag aaacaaaaga taagcgcttt tcttttaatt gttcagctag
     2881 ttgttttaaa tcggcgtgct tttcatcagg caagatgaca agatagattt gttttccttt
     2941 tcttgatttc aataatagat tttttacttg cggaccaggt agggaaaaag ggacattttt
     3001 gactgacgta atcgcaggat gatcgacttt ttgaaaagag atacccagtt tttttagtag
     3061 ttcataagct tcttgttcgg ttgcacgact catcgcgcat caccctcctt tttttattag
     3121 tgtatcataa aaaagagttg acaaatgaaa aataatcatg taaattacac gtaaagttaa
     3181 aacaaatgac atcgaaaaga agagtagccg ttaggtgttt ttagagagtc tctggttggt
     3241 ggaaagaggc agcagttaac cgtgaaacac atcttggagt cgattttctg aagcgtatag
     3301 taggaaaatc cggggagccc gttacagctg cggtcgttgc tagatgacca gataaggctg
     3361 gcttttccgt gaggagtcgg taaaaaaagg tggaaccacg tgattaacgt cctttgatct
     3421 tcggatcaaa ggactttttg ttttcatctg aacggcaacg actgtgtgag gttttttctg
     3481 caaggaaaaa ataaacagag gtggaaccac gtataatacg tcctcttggc atttgccaag
     3541 aggatttttt tattttaaag gaggaaaaga agatggagta tttgattgaa gttatgccgc
     3601 aattattagt aggagcactc acaacattga aattattttt atttaccttg attggctcat
     3661 taccattagg gattgtttta gcattaggat tgtctttgca ttttaagccg ctacgctact
     3721 tgcttcaaac atatgtatgg gttatgcggg gaacaccgct gttgttgcaa ctgattttta
     3781 ttttttatgg gttgccgacg attgggatcg tttttgatcg tttcgaagca gcactcattg
     3841 cctttatctt aaactatgca gcttattttg cagagatctt tcgtggaggg atacaatcga
     3901 ttcctcaagg tcaatatgaa gcagcgcaag ttttgcaatt aagtcccttt caaacggtga
     3961 cgaaaatcgt catcccacaa gtgacaaaga ttgttttacc ttctgtaggg aatgaagtta
     4021 tcaacttggt caaagattct tccttgatct atatattagg tttaggtgac gtaatgcgtg
     4081 ctgggaaaat cgcgatggaa cgcgacgcga ctttactgcc tttgctaggc gtggcaatca
     4141 tctatcttac attgactggt attctaacaa tcgtaatgaa acaagttgaa ggaaaattaa
     4201 attattaccg ctaactgata ggaggaagaa atatgattga attgaagaaa gtgaacaaag
     4261 catttggtga aaaaaaaata atcaaagatt tgaatctaat cattccagat gggaaaacat
     4321 tagctatcgt aggtccttcc ggtggcggta aaaccacgct tttacgtatc ttggctggct
     4381 tagaaagttc tgacagcgga gaattttatt tagacggtgt accgttcaac ccattcgagc
     4441 ttaaaatgaa agaacaagtt gtaggagtcg tattccaaga cttccagcta tttccacatc
     4501 tgaatatttt tgaaaacatt accttagcac caaaaatggt attgaaacag acaaaagaag
     4561 aatacaccaa aaaagcagaa gaattagcgg cttcattagg gattagtgat ttattgaata
     4621 tctatcctta tcagctttct ggcggacaaa aacagcgggt agcgattgcc agagcattgg
     4681 ctatgaatcc acgggtattg gcttatgatg aaccaacaag tgctttagat cctgaattac
     4741 gccagcaagt cgagcgtttg atcctatcat tgaaagaaga tggggtaacc caaattattg
     4801 tcacccatga tttagtattt gcagaaaatg tcggtgatca attactaaaa gtttccccaa
     4861 ttcattaaga aaggagaaga tagagatgaa aaaaaccagt tttctattcg ctttgatcgc
     4921 aggtcttctt ctttttgcag gctgttcgaa caaaaaaact tcagcagata attgggcgac
     4981 tatcaacgaa gagaaaaaaa tcgttatagg tttagacgat acatttgttc ctatgggatt
     5041 tagagataaa gacgggaagc tcacaggatt tgatattgat ttagccaaag cagtctttga
     5101 acaatatggg attacggtag actttcagcc cattgactgg tcaatgaagg aatttgaact
     5161 gaacaatgga acgatcgatt tgatatggaa cggctattcc aaaacgccgg caagagaaaa
     5221 gaaagtgcaa tttacaaaac cttatatgga aaacgatcaa gtgctgatca cacctaaaga
     5281 atcaggaatc acaaactttg gacaaatgaa taataagaga ttaggtgcgc aaaatgggtc
     5341 atctggttat gatgtattta cgaaacaacc agaagtatta aaagagatag tcaaaaacca
     5401 agatgctgtt ttgtatgata gttttaatga agcattgatc gatctgaaaa gcagacgggt
     5461 cgatggtctg ctgatggata aagtatacgc agactattat ttgaaacaaa gaaacgaact
     5521 agaagacttc aacataacaa aaggttctta tgagagtgag gattttgcag ttggtgcacg
     5581 caagtcagat agtacattag tgaaaaaaat caatgaagcc ctagatcaac tagagaaaac
     5641 aggtgaattc caaaaaatat ccgataaatg gtttggcgaa gatgttacgc cgagaaatta
     5701 acaaataggg gactgtgaca aaagacgtgt cacagtccct ttttccgaat aaacagtgtt
     5761 ctcctgccaa ctccttgtga ctcggagctg aatggcaggc tcacaacctc ttggtaaacg
     5821 gtgttcaaaa gccagttttt cggtattaag ccaaatttat gcaaaaatgt agaaagccat
     5881 tttcacaaaa tttttcttac tactcgaaac taagcgactt tttcacaacc tcctttacat
     5941 aattttattt tgttgtggaa ggggtttcgt gttattctaa aagaggaacg attgaaggtt
     6001 ataggaaagg aagagatgaa tggaacacaa agaacttacc tcatttccaa aagattttct
     6061 gtggggctct gcttctgcag cgtatcaagt agaaggagct tggcaagaag atgggaaagg
     6121 agaatcagtc tgggatcgat ttgtacggat acctggaaag acatttaaag gaacgaacgg
     6181 tgatttggca gtagaccact atcatcgtta taaagaagat attgctttaa tgaaagaaca
     6241 aggtctgaaa gcttaccgct tctctgtagc ttggacacgg atttttccta atggacgagg
     6301 agaagtcaat caagctgggc tcgcttttta tgagagattg atcgacgaat tgattgaaaa
     6361 cgatattgaa cctatgttga ctttatatca ttgggatctt ccacaagcat tgcaagatga
     6421 atataatggt tgggaatcac gtcagatcat tgaagatttc actcattatg cagaaacatt
     6481 atttgaggca ttccgcggga aagtccatta ctggattagt ttgaatgaac aaaatatctt
     6541 cacttcatta ggttatttgt tagcagcaca tccgccaggt gtgacagacc cgaaacgaat
     6601 gtatgaagtt aaccatattg ctaatttagc aaatgcatct gtcatcaata aatttcacga
     6661 gttagaaatg cctggaaaaa tcggaccaag ctttgcgtat acgccaaatt atccaattga
     6721 cagtaatcct gaaaatgttc tggctgctga aaatgcagaa gacctgatgg ccaattattg
     6781 gatggatgtc tatatgtggg gaaaatatcc gattgctgcc atgaggttct tagaagaaaa
     6841 aggatgggca ccaacgattg aagctggcga tgcagaattg ctagaatctg ctaaaccaga
     6901 ttttcttggg atcaattact atcaaacggc gacgaatgca ttcaatccat taaacggagt
     6961 tggtgctgga aaaatgaata cgacaggaaa aaaaggttca tcagaagaaa ctggagttcc
     7021 tggaatgtat aaaaaagttg agaatccatt tgtcgaacga acaaattggg actgggagat
     7081 tgacccagaa ggattaagga tcggactgcg tcgtatcaca agccgttacc gcatgccggt
     7141 actgattacg gaaaatggct taggagagta tgacaagttg acagaagata aacaaatcca
     7201 tgacgactat cgaatcaatt acttacaaag ccacgtcaaa gccatcaaag aagcaatttc
     7261 ggatggagca gaagtcctag gctattgcac ctggtcttat acagatttgc ttagctggct
     7321 gaacggttat caaaagcgat atggatttgt atatgttgat caagacgaaa cacaagaagg
     7381 atctttggaa cgatataaga aagacagtta ttactggtac caaaaactga ttgaagaaaa
     7441 tggagagaac ctttagttca catacttaaa acaagaaggt acggctggat atggccgtgc
     7501 cttttcttga taaaatcatt tagaaattaa gaaaaactgt aaaataagct gaaaaatgaa
     7561 agaaagcgtc agttctttca aacaaaactt tagttccctt actttttttt gtataatgaa
     7621 acttgatgaa atgagggata ataaatggca caaaagaaac ggcctactaa aaaaaggaaa
     7681 aagacgaaaa aaacgaaaaa gcaaaaacag cagcaggaac aaatgctgac gattggtgtt
     7741 gggtttgcct tgattttatt tgctgtcttc ggctttttaa aattaggttt tttaggaata
     7801 ttgattgcaa acggctttcg gatcattgca ggaaatactt accaaatcct ctgtctttta
     7861 ttagccgtat taggattttg gattgtcatt aagaatacag agttttcaat tggtaaaaac
     7921 cgacgctggt ttggcggcat actcttttat ttcggtatct tattattgtt acatgcccat
     7981 ttatttggca aactccatac tggggaacca aatatcatgg ggacgacatg ggatttgtta
     8041 gccagtgata taaaacaaag tcaagtagac aataacgttg gtggtgggat gattggagca
     8101 atcctttatc attttactta cttcttgatt gcacagcctg gaagttattt agcagcagtg
     8161 ctattactgg caggaggagc attcctgttc agtaatctgg aaggctatca gctgttaaat
     8221 ggtatccaat caattggaga acgagtacag gaattattgg aaggtgatcc tgccaagcaa
     8281 gctcgaaagc aagcggcaaa ggaagagaga atgaaacagc gcgcagaagc gaaagaagca
     8341 aggcgcttag ctgcaaaaga agctgcagaa aaagaagctg cagaatatga gaagaaaaaa
     8401 gcggtgagcc agcaaaggga aaatactgct gatgaatggc aggaaccaga aaatacagag
     8461 ccggagcagc taagttttgt tccaatcgac agttttcaag aaaatatcca tcctgcgaat
     8521 ctagagaaac cagtaccaga tacaccaaaa cagaccaata cagcagaagg tttttcagat
     8581 gagttgccag aggatgacgg tacctcatta gaatttgaga ttgaagcaga acaggaaaat
     8641 caagactatg aattaccgac agttgatttg cttgattcta taccaactgt ggatcaaagt
     8701 gatgagtata aaaaaattga aaaaaatatc ggcgtattag aacaaacttt ccaaagtttc
     8761 ggtgtcgatg ccaaggtcgt caaagccagc ttaggaccat ccgtcacaaa attcgaagtc
     8821 cagccggctg taggggtaaa agtaagtaaa attgtgaact taacagatga tatcgcgctt
     8881 gctttagcgg cgaaagatgt ccggatggaa gcaccgattc caggtaaatc actgattggt
     8941 attgaagtac caaatggaaa gatcagtatg gtttccttta gagaaatcat cgaagcacaa
     9001 ccaaaccacc cagataaatt actagaagta cctttaggac gtgatgtttc tggacgggta
     9061 caaacggctg atctttcgaa gatgcctcac ttactcgtgg ctggttcgac tggtagtggg
     9121 aagtcagtag caattaatgg gattatcaca agtatattaa tgcgtgctaa gccccatgaa
     9181 gtaaaactga tgatgatcga tccgaaaatg gttgaattga atatgtacaa cggcatacct
     9241 catctgttga cgccggtcgt gacgaaccct agaaaagcag cgcaagcctt acaaaaagta
     9301 gtacaagaaa tggaagaacg atatgaaaaa ttcgcggcaa ccggagtaag aaatatttct
     9361 ggttacaatg aatttgttca acagaaaaac ttggaaaacg gaacgaaaca tccaacctta
     9421 ccattcatcg tagtgatcgt agatgaatta gctgatttga tgatggtagc cagcaatgaa
     9481 gttgaagatg cgattattcg tttagcgcaa atggctcggg cagcggggat tcatatgatc
     9541 ctagctactc aacgtccaag tgtggatgtt atcacaggaa tcatcaaagc aaatgtacct
     9601 tccagaatgg catttgcggt atcaagcggt acagattctc ggacgatcat tgattctaat
     9661 ggggcagaaa agctcttagg gcgaggagat atgctattct tgccaatggg tgagaataaa
     9721 ccgatccgtg tgcaaggagc attcatttct gaccacgaag tagaacgggt cgttcaattt
     9781 gttacagatc agcaagaagc tcattatgaa gagaaaatga tgccgacaga tgaagttgaa
     9841 acgacaggtg ctccagaaca gccacaagat gagctgtttg aagaagcaaa agcacttgtc
     9901 gttgaaatgc aaacggcaag tatttccctt ctccaaagaa gatttcggat cggttacaac
     9961 cgagcagcac gtttagtaga tgaactggaa gctcatggag ttgttggccc gtcagaagga
    10021 agcaagcctc ggaaagtatt cattgagcag gaagaaacat cagaaacgtt gccggacgat
    10081 aatgacatgt aaaacttgtg tgatgaaggg ttgaaaaacg aataaccaat aaaaagacaa
    10141 ggcatgatga taccaaaaga aaaaatggtt tttttccatc ttttctaata cggtatgatg
    10201 tcttgtcttt ttagtttttg tgagattaaa gttctattcg atttatttgt gaagtattgc
    10261 attaaggaaa tgaatcatgt atagtatgta gcgaaacttt gagaaaaaga gggaaaatac
    10321 tttggaaaaa cagtccattt atggcttgac gaatgaagaa ttaatcaatt ggtttataga
    10381 aaacggcgag aaaaaattcc gagctgccca agtttgggaa tggttgtatc aaaaacgtgt
    10441 aagtaatttc acagaaatga cgaatctatc gaaacaattg atcgaaaaat tatcggagca
    10501 ttttatcatc aatcctctaa aacagatggt cgtccaagaa gcttcggatg gtacagtaaa
    10561 atatttgttt gaactccctg ataaaaacat gattgagact gtactgatga gacaagaata
    10621 tggcttgtca gtttgtgtga cgacacaagt aggatgtaat atcggctgta cgttttgtgc
    10681 aagcgggctg ctcaaaaaga accgtgatct gactgctgga gaaattgttg cacagatcat
    10741 gatggtccaa cattattttg atgagcgcaa tcttggcgaa cgcgtttcac atgtcgttgt
    10801 aatggggatc ggtgagccat ttgataatta cgacaatgta atggactttt tgcatatcat
    10861 caatgatgcc aaaggattgg caattggtgc tcggcacatt acagtttcca ctagtggatt
    10921 agcacacaaa atcaaggaat tcgctaataa cggcttgcaa gtcaatctgg ctatttcctt
    10981 gcacgcgccg aataatgagg tgcgaacatc gatcatgcgg atcaaccgaa gtttcccaat
    11041 tgagaaattg atggaagctg tggatgagta tttagagaaa acaaaccgtc gaatcacgtt
    11101 tgaatatatc atgttgaatc aagtaaatga tagaccagag catgcgcaac aattagcaga
    11161 tctattaaaa gataagaaaa aattagcata tgtcaacttg attccatata atccagtcag
    11221 tgaacatgat caatattctc gcagtccaaa agctgatgta ttgagatttt atgatgtatt
    11281 gaagaaaaac ggcgtaaact gtgtgatacg taaagaacat ggtacagata ttgatgcagc
    11341 ttgcggacaa ttgcgaagca agcaaatgaa aaaaacagtt gccaaataat cgtatcctac
    11401 tcgttttttc aattaccata aaaggagcag ttttcttaat taaggaactg ttcaacttaa
    11461 ataagagaaa agattctata gcgaaagcta taaagctttt tttgacaaga aaaaccctca
    11521 tcaagcaaag aaatgtcctc aatttgatga cagcctgctt gatgggggtt ttagcaatta
    11581 tttaactgat tttcttatat ccttgctggc tttcttgtgt agcatgataa gtttctacgt
    11641 caagaatggc ctgtttaaat ttttctcggg tgatttcata aggcacatgt ttaatatcat
    11701 ttgttgccat ggctttatct aagattgctt ctaaatcggt cgtgacattg agattcatgt
    11761 cggttaacga tactggaagt tggttctttt tcataaacac atagatttta tcaaacaaat
    11821 cgaactgctg atccatcatc aataaggtca gtacaccata actgacagat gctccgtgaa
    11881 ggtatccctc cattttttct agaacagtcg taccgtaata gaatgagtgt gccatagcag
    11941 aattatagtt attgttaact aaaacagaaa ccaatccggt agtaacaatg atatccgaaa
    12001 cgacttgttc aattgcatat gagggacgat cgtcggcact atctttcaat gcttgcgtac
    12061 cataagtcaa gagacgatca atgcacattt ttccgatttg gattcctaat ctatccgtgt
    12121 agcctagatc atcgccacga gcagaaaaag tagattcgac ttctttggat aaagcatcgc
    12181 caatacctgc ccataagtat ttatcaggtg cttcggcaat aattgctgta tcaatgaagg
    12241 tgtgtacagg tggttttgaa ggaaagaaga gtccgtccat tacgccttct tcgttataga
    12301 tgacacatac cgcagtgacg ggcgaacagt tcgaaccaat agtgggaaag gtgaagactg
    12361 gtttagctag tttttcacca atgactttta cagtatcgca agcacggcca ccaccgactg
    12421 caaacaggac gtctgctttt tggacacttt catgctggat caactgttca atatttccat
    12481 aagttgaatt acccccgtac cagagactgg cgacaacctc tatttcctgt gtcaaagcta
    12541 cttttatttt gttttctgcc tttgaaagtg cagtcttccc tccaatcagc actacttttg
    12601 tcccatattc tttgacgatt ccatttgctt tttcatacgc atctgttcca atggtataat
    12661 ttggtaagaa aacactttga ctcatattca tttccttctt tcttcgttga aacgggttaa
    12721 aattttaaag agtgatcgat ctgactgcgg ctgtagatgg agtagaggtg aacctaagac
    12781 aggataagtg atgaataagg tggcatgaac acctt
//
LOCUS       DAYYKN010000080        12734 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000080 DAYYKN010000000
VERSION     DAYYKN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.080"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHBB7_001894"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHBB7_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM9209841.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACHBB7_001895"
     CDS             complement(323..892)
                     /locus_tag="ACHBB7_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM9209842.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACHBB7_001896"
     CDS             1078..1980
                     /locus_tag="ACHBB7_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9209843.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHBB7_001897"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHBB7_001897"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM9209844.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHBB7_001898"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHBB7_001898"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM9209845.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHBB7_001899"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHBB7_001899"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM9209846.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACHBB7_001900"
     CDS             8548..8733
                     /locus_tag="ACHBB7_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM9209847.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACHBB7_001901"
     CDS             complement(8795..9991)
                     /locus_tag="ACHBB7_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM9209848.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACHBB7_001902"
     CDS             complement(10161..11597)
                     /locus_tag="ACHBB7_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM9209849.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="ACHBB7_001903"
     CDS             11890..12699
                     /locus_tag="ACHBB7_001903"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9209850.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2673 c         2250 g         4010 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgac
//
LOCUS       DAYYKN010000081        12707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000081 DAYYKN010000000
VERSION     DAYYKN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.081"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1138)
                     /locus_tag="ACHBB7_001904"
                     /pseudo
     CDS             complement(573..1138)
                     /locus_tag="ACHBB7_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1317..1973)
                     /locus_tag="ACHBB7_001905"
     CDS             complement(1317..1973)
                     /locus_tag="ACHBB7_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209851.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(2541..3476)
                     /gene="whiA"
                     /locus_tag="ACHBB7_001906"
     CDS             complement(2541..3476)
                     /gene="whiA"
                     /locus_tag="ACHBB7_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM9209852.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(3510..4508)
                     /gene="yvcK"
                     /locus_tag="ACHBB7_001907"
     CDS             complement(3510..4508)
                     /gene="yvcK"
                     /locus_tag="ACHBB7_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM9209853.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(4505..5389)
                     /gene="rapZ"
                     /locus_tag="ACHBB7_001908"
     CDS             complement(4505..5389)
                     /gene="rapZ"
                     /locus_tag="ACHBB7_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM9209854.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(5524..6255)
                     /locus_tag="ACHBB7_001909"
     CDS             complement(5524..6255)
                     /locus_tag="ACHBB7_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM9209855.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(6259..7524)
                     /locus_tag="ACHBB7_001910"
     CDS             complement(6259..7524)
                     /locus_tag="ACHBB7_001910"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM9209856.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7787..10603)
                     /gene="uvrA"
                     /locus_tag="ACHBB7_001911"
     CDS             complement(7787..10603)
                     /gene="uvrA"
                     /locus_tag="ACHBB7_001911"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM9209857.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10613..12607)
                     /gene="uvrB"
                     /locus_tag="ACHBB7_001912"
     CDS             complement(10613..12607)
                     /gene="uvrB"
                     /locus_tag="ACHBB7_001912"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM9209858.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
BASE COUNT         3607 a         2831 c         2039 g         4230 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctgagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaaattccc ttaacgagta atatttatgt
      181 acggtgattc acactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcgtttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actcctaact cctctcattt aatagttgct ctctttttta ttcagataca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt aatagatttc gattattcaa agcgattttc tattggtgga
      601 ttgtgttccc aactaacaag acggattcca aagtcattat ataactcttc aagagttaac
      661 cctttcctgt tcccaataga tgtatctctc ccacgctcga tataaaatat ggagtttcga
      721 atgccaagac ctggcaaatc tttccttgtt acagttacac tgctactaac ttccccatat
      781 aaaatccagc tcctccaaaa tgataatata tttttcgacc atcatctaac tcatgtaaca
      841 ataattgttg tgccctcttg tcactcgcca aatgcaaacg ccacgtgcga taccatgcca
      901 tgctacctgc atctgcagaa aatccatcaa ataatgcaac aattgtatct tctatcacaa
      961 tcctttcccc atctacttca tattctaatc taaaatcaaa ctcccctcgg gtaatcgcag
     1021 gtcttggtgg cttaggaccc aatattattc tacttaaaac cgcattcgtt cccagatgaa
     1081 acacagctat aacaacaata atgattaata ttttgtttct atttgataaa tttctcatta
     1141 ttcgcaaatc acctctacat tctcacatac tagttcgtaa tactccttta atatcaaaat
     1201 atctactgat cgtaataata ttcgctatga attcctacta ctcgtataag aaaggccaaa
     1261 tcatcagctt ttacttgctg atgatttggc ttgtttctat ttatcgtaat tgttttctaa
     1321 tcaccaaaat tgttaacaat cggttcagat atttcccaat tcactagcct tatattatat
     1381 tgatttaaca attcttcttc atttattgta ttttgatcaa gcggaccaaa ttgttctaat
     1441 aattctttgt catgtaaaat atcccttcta gtaatgcctc tttccctttc aatgaaagaa
     1501 gcactaggaa aagtatgatt ataaccattc aacctatctc ccatgtaata attagcaggt
     1561 cctggaaaat aaaatatttg cttattatca ctaacttcta acagcaacac taaatcttct
     1621 ccgctggcta aacgacttgt ccatctacga tatctcccta tgccttcgtt cattccaaat
     1681 ccatcaaatt caactattaa cgtgtcttct atcacatgta attcttcatt caattcatac
     1741 tcaagtctaa aaggaaattc tccatatgta atctctggca atggtggtgc tggagttctc
     1801 caaataaata caataactgt gaatagaaaa aatccaacga tcgatataaa tgttatgaga
     1861 cctgctcgaa gtaaaacccg atataataaa aatgctgtat ttcgctttgc tatatctaga
     1921 aaaataaaaa gtactgcaat cataatgata gtaaaagttg taaaaaaacg catttttgcc
     1981 ctccctttat aaacatcgat tctcaaattc taaaccaaat cttggtagtt caagttcagg
     2041 tataacgtat tccaaccata catctcaatt actatttatg aacttatgat tatcactgaa
     2101 taaatcactc catccccttg ttaaaacttc tacattaatt acacctggtt gttttcgact
     2161 agctaatcca atgaacttat caaccatcct tatccaccaa ccacgagaat cttgaccaga
     2221 agtaagaaga gcagttctca aggatgatcg agcagttcct tccagttttt taattaatca
     2281 ggccagttat agataaatac ttgatcttat ctatatcagg acttctatat taagcaaaaa
     2341 tttatgtaca aaactaaaat cttcattcag ttgtctcccc gctttttata cgattgtatt
     2401 aaaaacaata gaagcctttc caaattatgt tactccagta aagatggaat tatagtttga
     2461 cggaagaaaa gcaagtataa cttttaaaaa aaactgatac attcgcatct cctgcttcat
     2521 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     2581 attttgcgaa ttcggtgatt aattcccgat tttgatatcg cacctgaggg aatcatttcc
     2641 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2701 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2761 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2821 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2881 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata agaaatatag
     2941 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     3001 tcttggttat gctgttcata aatagaaaat atttccaaat gataacggct cgtttccgga
     3061 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     3121 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     3181 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     3241 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     3301 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     3361 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     3421 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     3481 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     3541 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttccaaaaag
     3601 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3661 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3721 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3781 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3841 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3901 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3961 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     4021 atggttttac ggtcgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaag
     4081 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     4141 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     4201 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     4261 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     4321 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc cccatcatct
     4381 gcaacagtca ctactgctgt gatatctgct ccttgatttc gcaaactttt caggataact
     4441 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     4501 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     4561 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4621 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4681 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4741 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4801 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4861 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4921 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt cgtcgtatca atgatcacgg
     4981 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     5041 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     5101 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     5161 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg taaatccact cctaaagcca
     5221 tttttgtgac tttgcctgat tctttgatta attcccagaa ttttggaatc aatcgaggcg
     5281 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     5341 ctgcaccgct catacctgtt atgatgacta attgcaaatt atctgtcata attacgaact
     5401 cctctcacat tgtgcatagt aaaattatat cattccgggc gtatcattac cagttttctt
     5461 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     5521 tttttacttt tccgatgctg tatccaatgc cttgcttctt tcagctagcg ctcgttcaat
     5581 cgtccgatct gctaaaatag attgggcatc gatcactgga taatggttat cttcagcaaa
     5641 ttcatgcatc aatgacaatt ctgtgcaacc taagataact ttttcacagt ttaggcgctc
     5701 taccgcttct tctaagattt cgtaatatag ctcttgattc aaataatccg attccttgat
     5761 ctcatgatag atcagataat tgattttttc ttgaagaaca gtatcaggaa tcactgtttc
     5821 gaatccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5881 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5941 catatgaagg ataggaatat ccgttgccgc ttgtaattct tcaaaaaagt agtgtgcggt
     6001 gttacaagtc aacacaatga agttcggctg caataaattt tgtttctcaa tatcatccag
     6061 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     6121 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     6181 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     6241 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     6301 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgaccggt ctttttcata
     6361 aaacacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     6421 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     6481 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttaaaca ctcgatcttc
     6541 tgtgacgaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6601 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6661 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6721 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6781 taacagtgga tgaccaagac acatcatccg aacttgatga tcttcatcta catacgcatt
     6841 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6901 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6961 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     7021 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     7081 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     7141 gcttacttta ttgatcagtc tttcaaataa agaataatca atatatggac agataaaata
     7201 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     7261 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     7321 gataaacaca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     7381 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     7441 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     7501 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     7561 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aactcctccg
     7621 attgctcaat tcttttttga acggattgaa cttataggaa gtttttgtct ataccaatca
     7681 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
     7741 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
     7801 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
     7861 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
     7921 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
     7981 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
     8041 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
     8101 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
     8161 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
     8221 ctacagcatc ttctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
     8281 cttctaatgt ttcagagtta tatcgtttgc catgacatac ttcacaagga acatagacat
     8341 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
     8401 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
     8461 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
     8521 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
     8581 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
     8641 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
     8701 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
     8761 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
     8821 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
     8881 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcagat
     8941 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
     9001 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
     9061 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
     9121 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
     9181 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
     9241 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
     9301 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
     9361 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
     9421 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
     9481 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
     9541 aaagatcatc tgtcccattc aagatgatat cctgatgctc ttccggtaat tcttcgaatg
     9601 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
     9661 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
     9721 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
     9781 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
     9841 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
     9901 gagcatagcc gtctgccaat cgcagagccg cctctaaaga atcaaataga cgagaacgaa
     9961 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
    10021 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
    10081 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
    10141 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
    10201 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
    10261 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
    10321 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
    10381 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
    10441 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
    10501 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
    10561 tatgggcacg tgcaccgtga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    10621 aagcttttaa ttctaaaatc atatctcgca cattagcggc tgtttcaaaa tccaatgctt
    10681 tcgccgcatt tttcatttct ttttctaatt tcatcaataa cgtatctttt tcttctttcg
    10741 tcatttcttc atacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    10801 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    10861 tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc catcgctaga cgcatagaat
    10921 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    10981 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    11041 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    11101 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    11161 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    11221 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    11281 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    11341 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    11401 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    11461 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    11521 ttctcgctcg gtcaccatta tacattcctc tgatttgagg catggttacg tgcgattcgt
    11581 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    11641 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    11701 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    11761 tttcatttcg caagacagtt aaacgctgtt ctaattccgc tttgatattc gctacggcat
    11821 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgct acgtgttccg
    11881 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    11941 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    12001 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    12061 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    12121 cttgtttttg gtactcaaaa ggcgagccta atccaaaaat acatgaaacg gatgcaatga
    12181 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    12241 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    12301 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    12361 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatc agtgttggtt
    12421 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    12481 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    12541 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    12601 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    12661 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaac
//
LOCUS       DAYYKN010000082        12300 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000082 DAYYKN010000000
VERSION     DAYYKN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.082"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1480
                     /locus_tag="ACHBB7_001913"
     CDS             <1..1480
                     /locus_tag="ACHBB7_001913"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209859.1"
                     /translation="AHLRMQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSH
                     AIPKAGGMALFFPFISVMLLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDT
                     LRENSEEFQETIELQQEINSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSI
                     FSYVSLAVVILVGVHLLLTGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKI
                     QRIRAMKETSIQEGSDSPLKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGA
                     SGSGKTSILKLVSRLYDYDEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILE
                     NIRIGNSQASDEEVKKAARLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARA
                     FLKNAPILILDEITASLDAENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMD
                     QGKIVDQGTHSELYRRSEIYKNLIKKTKLSEKFVYEKEAQSR"
     gene            2003..2272
                     /gene="rpsN"
                     /locus_tag="ACHBB7_001914"
     CDS             2003..2272
                     /gene="rpsN"
                     /locus_tag="ACHBB7_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM9209860.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            2287..2466
                     /gene="rpmF"
                     /locus_tag="ACHBB7_001915"
     CDS             2287..2466
                     /gene="rpmF"
                     /locus_tag="ACHBB7_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM9209861.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            2498..2875
                     /locus_tag="ACHBB7_001916"
     CDS             2498..2875
                     /locus_tag="ACHBB7_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209862.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            2896..3045
                     /gene="rpmG"
                     /locus_tag="ACHBB7_001917"
     CDS             2896..3045
                     /gene="rpmG"
                     /locus_tag="ACHBB7_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9209863.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            3069..4052
                     /locus_tag="ACHBB7_001918"
     CDS             3069..4052
                     /locus_tag="ACHBB7_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM9209864.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            4082..4204
                     /locus_tag="ACHBB7_001919"
     CDS             4082..4204
                     /locus_tag="ACHBB7_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM9209865.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            4222..5745
                     /locus_tag="ACHBB7_001920"
     CDS             4222..5745
                     /locus_tag="ACHBB7_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM9209866.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            5769..6008
                     /locus_tag="ACHBB7_001921"
     CDS             5769..6008
                     /locus_tag="ACHBB7_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9209867.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6201..6764)
                     /locus_tag="ACHBB7_001922"
     CDS             complement(6201..6764)
                     /locus_tag="ACHBB7_001922"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9209868.1"
                     /translation="MDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRGKTRHT
                     KNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRKSCFLL
                     AGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNMPFYFP
                     DPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(6857..7075)
                     /locus_tag="ACHBB7_001923"
     CDS             complement(6857..7075)
                     /locus_tag="ACHBB7_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9209869.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            7264..8211
                     /locus_tag="ACHBB7_001924"
     CDS             7264..8211
                     /locus_tag="ACHBB7_001924"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM9209870.1"
                     /translation="MYGDHGKEIAVIVNDIGEVNVDGVLVDKKGFKRTGEKLVSMPNG
                     CICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPIIQAIILAKNPLDIDLTQRL
                     EIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDIRGLLIDQFECCNILLLNN
                     SDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILNTNLFNIDETSYSMGWFKE
                     LELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKNFPGNITRAKGFLWFAQHS
                     DFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            8178..8267
                     /locus_tag="ACHBB7_001925"
                     /pseudo
     CDS             8178..8267
                     /locus_tag="ACHBB7_001925"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8400..8807
                     /locus_tag="ACHBB7_001926"
     CDS             8400..8807
                     /locus_tag="ACHBB7_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM9209871.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(9048..10388)
                     /locus_tag="ACHBB7_001927"
     CDS             complement(9048..10388)
                     /locus_tag="ACHBB7_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9209872.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(10664..10819)
                     /locus_tag="ACHBB7_001928"
     CDS             complement(10664..10819)
                     /locus_tag="ACHBB7_001928"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFM9209873.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(10826..10972)
                     /locus_tag="ACHBB7_001929"
     CDS             complement(10826..10972)
                     /locus_tag="ACHBB7_001929"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209874.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(11003..11206)
                     /locus_tag="ACHBB7_001930"
     CDS             complement(11003..11206)
                     /locus_tag="ACHBB7_001930"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9209875.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            complement(11116..11448)
                     /locus_tag="ACHBB7_001931"
     CDS             complement(11116..11448)
                     /locus_tag="ACHBB7_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9209876.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(11962..12153)
                     /locus_tag="ACHBB7_001932"
     CDS             complement(11962..12153)
                     /locus_tag="ACHBB7_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9209877.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT         4108 a         1928 c         2382 g         3882 t
ORIGIN      
        1 tgcgcattta agaatgcaaa ttgcagacga tttgtcaaat ttaccattat cctatttttc
       61 aaaacataat ttatcagatt tatctcaaac tatcatgtct gacgttgaag gtattgagca
      121 tgcgatgagt catgcaatac ctaaagccgg cggtatggct ctgtttttcc cttttatttc
      181 agtgatgctt ttggttggta atgtcaaaat gggattagct gttattttgc caacgttatt
      241 tagttttgtc ttaatcttgt tatcaaagaa atcccaaacg aaagccaata ctaaatatta
      301 cgatactttg agagaaaact cggaagaatt tcaagaaact atagaattgc agcaagagat
      361 taatagcttt aatctatcta aaaaagttca agacagactt ttcaaaaaaa tggaagagag
      421 tgaaaggatt catttaaagg tagaattaag tactttttca gtcatggcct tatcctctat
      481 tttctcatat gttagtttag cagtagtcat tctagtaggt gttcacttac tgttaacggg
      541 tgaagtgact atactctacg tcgttggtta cttactagcc gcaataaaaa taaaggattc
      601 cttcgattct atgaaagaag cagtgcttga aatattttat ttagctccta aaatacagcg
      661 aattagagct atgaaggaaa ccagtatcca agagggatca gatagtcctc tgaaatcttt
      721 tgacattgag ttgcgagatg tctctttctc atatgataac aataccccaa ttttagatca
      781 tatctctttt acggctaaac aaggagaagt gactgcgtta gtaggtgcta gtggttccgg
      841 gaaaactagt atcttgaaat tagtgtctag actgtatgat tatgatgaag gctgtatttt
      901 aattgatggg tatgatatta aacgtgtatc tccagcttct ttgtttagca aaatcgctat
      961 tgtttttcaa gaggtaactt tgtttaatac aagtattttg gaaaatatac gtattggaaa
     1021 tagtcaagcg tcagatgagg aagtgaaaaa agcagctcgt ttagctaact gcgaagactt
     1081 tattgaaaaa ctacctgacg gttatcatac attggtcgga gaaaacggaa gttcactttc
     1141 tggaggagag cgacaacgcc tatccatagc acgggctttt ttgaaaaatg cgccaatttt
     1201 aattttagat gaaattactg ccagtttaga tgcggaaaat gaaaaacgta ttcaggaaag
     1261 tttaaatcgt ttaatacaag ataagacagt actaattata tcgcatcgct tgaagtctat
     1321 tgaaaaggta aataaaattg tagtgatgga tcaaggaaaa atagttgatc aaggtactca
     1381 tagtgagttg tatcgaaggt cagaaatata caaaaatctc ataaagaaaa ctaaactatc
     1441 tgagaaattc gtctatgaaa aagaagctca atcacgatga tgaaataggc tttaaattaa
     1501 cctacaattt ttgggatata gttataattc tttttcaaaa agaataattt tattaaacag
     1561 catctaattt tgaatacacg aaagaatcag tatgattgaa ttagttcact taatgtctat
     1621 tcttataata taaggaataa tcaaagtttt aaaaaattgg cgataaacca gttaaacata
     1681 aaagattgaa taaaaagtta tagtttcttt catattgccg ttttaagtag aggaaaagct
     1741 acttaaaacg gtatttgtgt ttgattggac taataaaagt gaattcttat ttttcagagc
     1801 ttaaactgca tattctttta aatgatttga ttatctctat gtaccgataa attcgttttc
     1861 aaatagttat ctattcatat gagttttatt tcatgttatt tattgttgac aagagcaact
     1921 cttgttggta aagtatcagt gtcaaaacgt aacgattacg atttgagatg tctaaatcaa
     1981 aataaaagga gtataaaggt atatggcaaa aaagtcgaaa attgctaaag caaagaaaca
     2041 aatggcgatg attgaaaagt acgcagataa acgtcaggaa ttaaaagccg caggagatcg
     2101 caccgctctc gctaaattgc caagagattc aaatccgaat cgtctgaggc ttcgtgatca
     2161 gacggatggt cgtccaagag ggtatatgcg aaagttcggt atgtcccgta ttaagtttag
     2221 agaacttgcc catcaagggt tgattcctgg agtaaaaaaa gcaagttggt aaaaggagga
     2281 aatatcatgg cagtaccagc aagaaaaaca tctaaggcga agaaacggtt aagaagaact
     2341 catcaaactg tagtaaaacc agaaatttca tttgacgaag cgaatggtga ttatagacgt
     2401 agccatcatg tatctttaaa agggtactat aatggaaaga aagtaattaa aggagcttca
     2461 aaatagttta atatataact tttaggaggg aatcaaaatg gaaattattt acccaccttt
     2521 agttgaagaa ggattaaagt attatcttga aacgacccag caatcgctag ataaatgcac
     2581 tttttatcgt tcgatggtag agagaggaat tattactgaa acgggtttac cgacccaaca
     2641 ggcaattgaa aatggattag tcaaagatta ctatgaggac caaggcttat catttgatga
     2701 ttttttaaga atctatccaa tttttgaaga gtatgacgag gagttatttc aatgtattga
     2761 tggttattgg gagatacctg ttgatatgaa agaaaattta gtttcacaat tggaatctgg
     2821 cgagttaact tttgaagatg cacaacaaat tcaagcatat cttgaagatc gataagtatt
     2881 gaaggaggaa gatttatgcg tcaaaatatt attttggaat gtgtcgaaac tggagaacgt
     2941 ctgtatctca ccagcaaaaa taaacggaat aaccctgaac ggttagaatt aaaaaagtat
     3001 tcgccaaaat tgcgcagacg ggcaattttc aaagaagtga aatagctaag tggtaaaaag
     3061 gtgacaaaat gggcatacca gttacaatcg tttctgggtt tttaggggct ggaaaaacaa
     3121 cgctaatcaa tcaagccttg caagtctcac cctaccccag ggaagaaatt attataattg
     3181 aaaatgagtt tggtgaagtt gggattgatc acaaattatt agttcatagc gaggaacggg
     3241 tgttacaact aaataatgga tgtatgtgtt gcagtcttcg cagtgattta ttagcaatat
     3301 tgatttcttt actggaaaca atagaagaac atcaacaacc aatttcacaa atcatcattg
     3361 aaactacagg tatagcagat ccccaaccaa tcattcaaac acttttaaca gcgcctcaac
     3421 taagaggaag agtatatatc gatagtttat tgactgttat tgatagtgac agttttgaac
     3481 ttattgaaag agaaccacaa gcccttaagc aacttgtcat agcagatcgc atttttgtgt
     3541 ctagaaaaca aaacttgaat ttatctgcac taaaaaaatt acagagagaa atcaaaagta
     3601 ttaacccttt atccgatatt cgaatgttca cacatagtga aacgattcaa aaaaaggatt
     3661 tttataattt ggaaaaattc aatcatccct tagtttttga aagtgattca tcagaaaatc
     3721 actataatgg caaacactat catcaacatc atcaagaaca tgagtttaaa tcaattctct
     3781 taacttcaga tcaagacata aaaaaagatt ttttattcca attaattgac cggctgattt
     3841 taaccaacca agggagcctg tatcgtttta aaggattgat tgctttgcaa gatcaagatt
     3901 ttatagttgc catacaaggg gtaaacgaac aggttaattt tcaatacacc actcgacaaa
     3961 aagaccaaac cactattatt ctaattggaa aaatgttgga tgaagaaagg atcaaacaga
     4021 gttttgataa attactggcg gaaagcaaat aatgataagt attgattagg ggtaatttct
     4081 aatggagaaa acggatatat ctagtgcata tcgacgactt aaaagcccga atatcaaaac
     4141 tagaaaaagg gcattaaaaa ttatcaaaga acataaacaa aataaaatga agaaattagc
     4201 ataattaagg aaggaaaaag aatgaagaaa atcagtgtag gacttattgg gatagcagct
     4261 ctaggattat tgggagcttg ctccagcact aatgatgcaa aagtatcgaa tgataaagat
     4321 ggaaaattag aaattgtaac aacattctat ccaatgtatg atttcaccaa aaacatcgtc
     4381 ggcgatgaag cgaacgtgga tttaatggtt ccagcgggat cagaacctca tgactatgag
     4441 ccctcagcta aggatatggc aaaagctcat gatgctgatg tttttgtata tcataacgaa
     4501 aacatggaat catgggtacc aaaagctaaa gaaagttgga agaaagctgg gcctaatgta
     4561 gtagaaggaa caaaagacat gattttattg ccaggtagtg aagaagaaga tcatgatcac
     4621 ggagaagaag atcaccatca tgaactagat cctcatacgt gggtttcacc caaaatggca
     4681 attaaggaag tttcaaatat caaggatcag ttagttaaat tgtatccgaa aaaagcaaaa
     4741 gtatttgaga caaatgctga aaagtatttg actaaactga aacggttgga tgctgactat
     4801 accactagtc ttaaagaagc gaaacaaaaa agttttgtga cacaacatgc agcctttgga
     4861 tatttagctt tggattatgg attgatacaa gttcctattg ctggactaag tcctgaagaa
     4921 gagccatctt ctggtcgatt agctgaatta aaagagtatg ttaaaaaaaa taagatcaat
     4981 tatatttact ttgaaaagaa tgctaatgat aaaatcgcaa aaactttggc caatgaagct
     5041 gggataaaat tggaagtatt gaatcctctt gaaagtctta caaaagaaca aatggataat
     5101 ggtgaagatt atgtttcagt aatggaagac aatctaaaag cgttagagaa aacaactatg
     5161 gttgctggtg aagaggtagt tccagaaaaa gaagcgaagg atgaaaagac agttgctaat
     5221 gggtacttta aagatgccga tgtgaaggat cgcgaattga gtgattatac aggcgaatgg
     5281 caatctgttt atccgttgtt aaaagatgga attttggatg aagttttcga ttacaaagca
     5341 aaactcaata aagacatgac agctgccgag tacaaagatt attatactac tggctataag
     5401 acagatattg atactatcaa cattaaagac aacaccattg attttgtagt caacggtgaa
     5461 catcatcaat acacatacaa atatgttgga tacaaaattt tgaattatga aaaaggaaac
     5521 cgtggtgttc gttttaattt tgaaactgat gatgcaggtg ctggtcgttt taaatatatt
     5581 caatttagcg atcatggaat tgcaccaagt aaggcagagc atttccatat tttctttggt
     5641 ggagaaagtc aagaaaaact ttataatgaa atgcataatt ggcctacttt ctatccagct
     5701 tcattatctg agcatgagat tgctcaagaa atgatggccc attaaaatcc aatctttgga
     5761 ggaaatacat ggtaagaaaa gtattattgt ctatggattc caagaatatt gatgcttatc
     5821 ggcgttatca atttgcaaag caaatgagat taaatgggag tacattagct gatattgctg
     5881 caactttaca tgttagtatt gacacagttc atagagtatt aaaacgggat cctgattgtt
     5941 gttttatcaa ggtcaatact ggaagaagta gacgtttagc aaaacggcat aatagcttgg
     6001 ccaattaatt gataatacca gagtagatca tttttccctt atttggattt gatctgcttt
     6061 ttattatttc ttgaattgtc aaatcaagcg caacacttta tcaaagaaga cttcataagg
     6121 tgttctccaa tctaaacact ttctaggtct gttattcagt ttttcaatca tctgatttat
     6181 cgttgagtta tctaccgtag ctatctcttt cgattttggc aagtattctc gaatgagtct
     6241 attcgtattt taatttgttc attgttgcca agggcatgag gatctggaaa ataaaagggc
     6301 atattattca atacttctgt aactgcgaaa tgattggaga attcttttcc tctatctgga
     6361 gtgatcgttt tgaccgcctc atttggaata ctgcttaaaa gttcaatcat tttctcgctc
     6421 acataagcgg ctttctttct ggggaccttc tttcctgcta aaaggaagca tgattttctg
     6481 tctgtcaaag tcactaaaca agtaccacta gttttgccag ctacaatatc ggctttccaa
     6541 tgaccaaaat cacttctatc atctgcggat tttggatggc catggatcga atgagtaagg
     6601 caaatcttcc cacgagactc gacatgattt tttgtatgtc ttgtttttcc tcgatggtgc
     6661 aatgaacgga tcaggccttt attgccgtgt gagaggggtt ctgtatcgaa aagatcattg
     6721 caaattgcac ggtagatcgt gataaaacta attgaaaagt ccatatgttc atgcttcaaa
     6781 cgattcgaga ttttctctgg cgaccactga aaatcgacaa ataaatgacg aacaattgac
     6841 caaactcacg gatcatttag aatgtgataa cgtccgcatt tttttcttcg ataagcataa
     6901 tttttttccg ctttggaagg tgattagttt ttttcttgtt tcttctcagt tcatgtaaaa
     6961 tagtaaacgg gctatgctga atgtcgttgc caattatact aatagaaaat ccttcttcgt
     7021 gaagcagaaa gattctttct cgtttgagta tagtaagatg acaatactgg ctcataatta
     7081 ttttccttct aggagtgttc tcgacaaact tatactagtt ggatatcgat tatgactcat
     7141 ttttatgtgt tgcacttaaa gtgtaaattc aaggtataga aagaggttcc ctatgaaaaa
     7201 atacctatta caatcttaac tggctatctt ggttcaggaa aaacgatact tctaaatcat
     7261 attctgtacg gtgatcacgg aaaggaaatt gctgtcattg taaatgacat aggggaagta
     7321 aatgttgatg gggtattagt cgacaagaaa ggttttaaaa gaactggaga aaaacttgtt
     7381 tcgatgccga atggatgcat ttgttgtaca ttacgcgaag atttgattgt tgaactagat
     7441 caattagtgc aattttctga cttgaatcaa attgttattg aagcttcagg aatcagcaaa
     7501 ccaattccaa ttattcaagc aattatatta gctaaaaacc ctttagacat tgatttgacc
     7561 caacgattag agattggtgc cattattata gtagtggatg taaaacgttt ttgggataat
     7621 ttcgaaacgg gtgaaaagat tcaggaaaga aaagctgaag gtaatgtagt tgaagaagaa
     7681 caggatattc gtggcttgtt aattgatcaa ttcgagtgtt gtaacatatt acttttaaat
     7741 aatagtgact tagtgtcaac caagacgatt tacaaaatta acggtctaat tgctaagtta
     7801 cagtccaatg ccaaaataat taggacaatt aaaggtgaag tagacttatc gaaaattcta
     7861 aatacaaatc tttttaatat agatgagact tcctattcaa tgggctggtt caaagagtta
     7921 gagcttggct atcaaaatca tattcctgaa acagaagaat atggtatctc aagttttatc
     7981 tatcgaaatc ggttactcct tgacgcacaa aaatttgccc atcttacgga taaaaatttt
     8041 cctggcaata ttacaagagc aaaagggttt ctttggtttg cccaacatag tgatttttcg
     8101 acgatctttg aaacatcggg tagccataaa agtattgaat cattaaatta ttgggtagct
     8161 agtttaccta aaattgagtg tcaaatgatt atacaaaaaa acccgaattg aaggaagcct
     8221 ggcatccgtt gtttggtgaa cgcattaata aactagtttt tattggtatg aagatggatc
     8281 aacaatgtat taaggaacaa cttgagggtt gtttaactga ttctcttaat gcagataatt
     8341 tccgaaaaat ttcaccattt ccatggaaaa attctaatat gtttagccag atatcgttaa
     8401 tgaaattttt aagaaaagct ggtgtcaaga taggcgatgt cataattatt ggcattttga
     8461 ttattccctc ttttattccc ttatttgtat tctctataga aaaggagaat gaaacaactg
     8521 aagatgctat caaatatgcc atcgttcaaa ttgatggcaa ggaaatggat cgcttcgatt
     8581 tggataagat caatcataaa ctcgtgaatt atcatcctgc gaagggacaa tataatattg
     8641 ttgagattaa agatggaaga attcgagtta aagaagataa tagtcctgat caaatcgcgg
     8701 ttagaactgg atggatctca aaacccggtc aaacgagtat ttgtttaccc cataagttag
     8761 ttattagtat tgagaaaaaa gaaagcaaag attactacat ttactaatct ggaaatagat
     8821 gaggaggttt ttaataagaa aaagatatta ttattaataa tagcaggggc tttattaagt
     8881 gtgttaagcg cttgtggaca agaaaaagaa gcggtgacaa caggcacaag tatctttaac
     8941 gggacctgca atggtttagt taacagtcgg ttagattact tattttggct cttcgtcaat
     9001 gagtgttggt gaagaaattc aatagaataa tcgagatact cacacggcta gacagctatc
     9061 ttgtctagcc gtgttgttgt tctggcttta gtcagttggg tagtgttctt agttttcttg
     9121 aaatattttc ctcgatataa aaagattcgt tctcggtagt tttgaaagtt tctaaagcca
     9181 taggcaattc gttttagtac tttgatatgg ttgttcaaac attctatagg gccgttggaa
     9241 tagggaactc gcaacgctcg tttgatttct gttcgatatt tcttgaaggt cgtgaacact
     9301 ggttgatagt gctctggcaa ctccttgtag tcttctttta ggagcgcgac gaattcagga
     9361 acatcttgtc ttctgatagc ggaaagaaaa acctggtaga cttcgtagcc tcgctttaag
     9421 gagtcatcat aatataatag ccgatcgact atctccgctt ctgtcagatg atctcgaaat
     9481 gacggacgcc agtagcgatg ctgaccattc aattcccacg cattcttttg aagtagtctc
     9541 cagtatttct ttaagtgctt cgaccgacga tccccttttt taaaggtctt cattacttga
     9601 attcggtggt tggtaaaggc ccgaccgatg tgttggctaa tatgaaagcg atcggcgacg
     9661 attcgggcat tcggaaaaca ctctttcacc aatgtgcgat agggtgtata gaaatctgaa
     9721 accactagac gtacttgttc acggactttt ctagggtagc gcaagaaata gtcgcgtaaa
     9781 ggattgagtt gccgattttc cacaatatca atcagttgat gggtcttgcc gtccatcatg
     9841 acaaagctca tggctcccga gacgtttttc accgatttga attcatcgaa acacaccact
     9901 tctggtaacg gctggttcaa agaaacggtt ttaggccgaa ggctgcgaag tattcgaatg
     9961 acagtggtcg gagaaatgtg cttcatacgt gcaatcaaag acatagaaat aggctcactt
    10021 aatagctcaa gaatggcttg tttgactctg cgggcaatgg agcagcgacg atcgctaact
    10081 gaatcttctg ctaaaaaagt ctttccacag tgtttacatt tgaagcgctg tttgttaatc
    10141 cgcacaatgg tcttgtagtt actgacatca ttcaataaga tggatacttt tttccagccc
    10201 catttaatga tcgtttgctt attttgatag tggcaatgga agcattcctc tggttgatag
    10261 gtcaatgtgc catcaaaaac aaaacatgtt tcgtcattaa tgcgctcttt gcgaaaggca
    10321 tcttcatgaa aggtaagatt taggtctaga atatctagta actctttggt aaactctgaa
    10381 taggacatct tgaaacactc ctttgaatgg ttttcgtcga ctttattcta aaggatttca
    10441 aggtgtctgt ttgcgtttat tagaaaaaag aaaaagtatt ggcaacagaa ggcgtcacca
    10501 acactagata ttatagatcc cttattttct tcgcttcaaa ttgtcaagga gtcatcataa
    10561 attttgtatg gattcttttg tttgggtagt agtcttctaa ataatcttgg attaacacct
    10621 tcatctctat tttactttta tatttgttgg agtcgattaa ttctcatttg attgatcgat
    10681 gaaacgcttc aattactgca ttgtcaacag gagtcccagt ttggctcatt gaatgtgtga
    10741 tgtggtggtc ggtcaacagc tcttgataaa taaaagaccg atgctggcta cctctatcgg
    10801 aatgaattac cgtaggcaat tctccttaat aaatttgtaa tggacggacg acaatctctt
    10861 ttttcatgtg tgaatccacc atatagtaac gtagaatacg agttgctaag tcaatatagg
    10921 tggacaaata gaggcgaccg tcattacatg gaatgtaagt aatatccgtc acccaaacag
    10981 catcaatttg gttctgttca aattatcgat tcaacatttt ttttaccatt tctttgattg
    11041 ttttttctct accatgagaa gatttgcgtc gatgaaatcc tcttgtgcat aaattcattt
    11101 cacgcataag aacagctaca acacgtttat tgatttcgat tccttcatcg taaagttttt
    11161 gagtgatccc tggagatccg tatcgatctt tgtgttcgta gaacactcgt cttcctcttt
    11221 tagtcgttta ttttcctgtt caagaatgta gaaatctgct ttatcaggta atctttttcc
    11281 ttgtccagca aaagcatttt tcccgtatcg acgatattct gaaacccatc gggaaattgt
    11341 tggacgagag atattgtatt cttcagcaat tttcttggta gttacttttt tctcaaggat
    11401 atcctgtgaa atctttagtt taaatttacg atcatatgtt tttttcaata gagttcattc
    11461 cttccggtaa cgtaactgat agtcaagaaa agtgtatcat agcccaactt agtatcaggg
    11521 tttgtcaact aaactgtgga agttgataga taagagtttt taacaaccaa aattctcttg
    11581 actatttttg aaatcagaca aattttgatt ggacacatag ttgaataaat ctaccgtcgt
    11641 tacggaaggt aaatcgcatg cttttcaatt ctagaggaga agtcctcgtt gaagtaccat
    11701 ggcccagttc tggattcgac ctcctgccca cttcgtacgt aaataaagta attttaggag
    11761 tgagactgtg acaaaagtta tttttaagct aaaaacgaat taaaaaacgt taataattaa
    11821 aatcattcgt tttagattct atgaatcatt atagaacgct gtaaaatcga tactttataa
    11881 ttataaagaa gatggcttca aatttattgt tcgaaatttg aaactatctt tttacttatg
    11941 tcacagactc ccttagttga gttattcttt aatttcgaat ttcgagatag ctagatatat
    12001 tgcaagcata cattcttgat aacaatcttg atcatatttt ccagttctcc aggattgctt
    12061 atggaccaaa gcgctaaatc ttttgagaat ggccacaact gcttcttcat ttccattctt
    12121 tgctaaaatg aaatcttctt ttaaagtatt cataaattta ccttcttccc atgaacttat
    12181 aatctattta ttttttgggg aaggaggagg acgagcaaag aagcatacaa aattagttat
    12241 tatgcagata ttaaaccagc attttttgaa tttttttgta aagtcgatgt tttaaatttg
//
LOCUS       DAYYKN010000083        12234 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000083 DAYYKN010000000
VERSION     DAYYKN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.083"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..455
                     /locus_tag="ACHBB7_001933"
     CDS             <1..455
                     /locus_tag="ACHBB7_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209878.1"
                     /translation="EMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTE
                     DGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVT
                     VKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(722..1891)
                     /locus_tag="ACHBB7_001934"
     CDS             complement(722..1891)
                     /locus_tag="ACHBB7_001934"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9209879.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            2095..3270
                     /gene="mltG"
                     /locus_tag="ACHBB7_001935"
     CDS             2095..3270
                     /gene="mltG"
                     /locus_tag="ACHBB7_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM9209880.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            3406..3885
                     /gene="greA"
                     /locus_tag="ACHBB7_001936"
     CDS             3406..3885
                     /gene="greA"
                     /locus_tag="ACHBB7_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM9209881.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            4054..4785
                     /gene="liaF"
                     /locus_tag="ACHBB7_001937"
     CDS             4054..4785
                     /gene="liaF"
                     /locus_tag="ACHBB7_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM9209882.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4782..5849
                     /gene="liaS"
                     /locus_tag="ACHBB7_001938"
     CDS             4782..5849
                     /gene="liaS"
                     /locus_tag="ACHBB7_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM9209883.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5856..6488
                     /locus_tag="ACHBB7_001939"
     CDS             5856..6488
                     /locus_tag="ACHBB7_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9209884.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6628..7293
                     /locus_tag="ACHBB7_001940"
     CDS             6628..7293
                     /locus_tag="ACHBB7_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM9209885.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            7321..7614
                     /locus_tag="ACHBB7_001941"
     CDS             7321..7614
                     /locus_tag="ACHBB7_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM9209886.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7682..8629)
                     /locus_tag="ACHBB7_001942"
     CDS             complement(7682..8629)
                     /locus_tag="ACHBB7_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM9209887.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8807..9610)
                     /locus_tag="ACHBB7_001943"
     CDS             complement(8807..9610)
                     /locus_tag="ACHBB7_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM9209888.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9756..10670
                     /locus_tag="ACHBB7_001944"
     CDS             9756..10670
                     /locus_tag="ACHBB7_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9209889.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10718..11188
                     /locus_tag="ACHBB7_001945"
     CDS             10718..11188
                     /locus_tag="ACHBB7_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM9209890.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            11273..11881
                     /locus_tag="ACHBB7_001946"
     CDS             11273..11881
                     /locus_tag="ACHBB7_001946"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM9209891.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            12015..12215
                     /locus_tag="ACHBB7_001947"
     CDS             12015..12215
                     /locus_tag="ACHBB7_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9209892.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         4142 a         1875 c         2437 g         3780 t
ORIGIN      
        1 aagaaatgaa ttttcaagga acttattcta atgaacaaat ggaaaataaa cttgaagaat
       61 ttgagaattt tgattatgta cctataggct ataaaaaaga tcaattgctt ttgaaaaacc
      121 atcaagttac agaagatggt caagttattt atagtagctt ttctccaaat aaagatgaaa
      181 ttacatttac gtttaaatct tcaaaaggca aaacaatagt tttacctgta ttccggtata
      241 aaggattaac ggttactgtt aattcaaaaa atgctacagt actaaaacaa ggaggtcctt
      301 ttgttacagt aaaaagtgaa agtggaataa ataaagtaac aatcaggtat tcatacacat
      361 ttttttctaa aatctcatta tttatttcta taatcacgct aattgttctt cttttttatt
      421 gcataaaatt aaaaataaga acaagccatg aatgatttgc tagcacatat tttagcaatc
      481 taaagatact attttagatt taataggccc tttgtcaaat aatgttggta aagagaaatc
      541 ttagaataat ttggacagaa cagaaagaag aacacctctt tctgttctgt ttatttggaa
      601 ttaaagtctg tagcaaaaat tgatttttag atttgtccca cactctttcc tacgtttagt
      661 cgagtacttt ttttaagcaa atgctattca aagaagtagt atttgctatc ttttcatcat
      721 atcagctatt tgccaactta gtagttcctt ccatcaggtc atagatttgg tttcttagat
      781 cagcagtagg cagtttgata gttttgtcag cttttatatt gctttccatg atgatagcat
      841 cctgtgaatc tttgctaaat atagcagatg tatcaaaaga gtcgatattt ccttgcgaca
      901 ggatataatt atctccgcta tataccccac cactatatgg atgtccattc gcattttcac
      961 gccataatga gaagagtaag tctttggaaa ctagttttcc agataaaact tgtgtaaaca
     1021 gcgtatttaa atcatagacg gacatactga tattaccagt ccctaattcg ttatggatct
     1081 ctgtttcaga ttctgtcagt ggtttaaagt aatcatcctc tttagacatt tgataggata
     1141 gtgcatgatt ggatgaattt ttgacttggt cataaaattt tgtatgctgt aatcctaaag
     1201 gttcgatgat atcatcggtc aataattttt catacggttg atttgttaat ttgatcaaaa
     1261 ttccagtcaa tatggtaaag ttcaatgaat cataatgaga ggtctgataa tctgtaaatt
     1321 ttaagtgctg aatcgcaaat tgaacgactt cttcatcagt catcggtatg gttggagaag
     1381 cagttcggtg caagcctgag cgcatataca gcaagtcacg gatagtaatt tgctggctgc
     1441 cagcaatttg tggatagaat tgagccagct tagtatctaa agaaaggtgc ccacgctcga
     1501 cttgttttaa aatcaaaaga gtagtcaatg ctttttgaac cgaaccaatc tgataataaa
     1561 gagaaggatc atttggttgt tgcgtgagtt tattcgcata gccatagcct ttttccatga
     1621 tgatgcgatt atttttcttt acgtaaattg agccaatata atcatttttt cttaatagtt
     1681 gatcgatttt ttcaacgagc tgttgttcat tggtagtcag tatcaacttt ttttgagctg
     1741 attgagcatg ggcaactatc tgagagtgtt cattattgta aaattgccat ccaagaatag
     1801 ttcctgtaga gaacgcacta aggctaaaca acacgacaac gatcattaaa aatgaatatt
     1861 ttcttttttt agcatgtttt ccttttttca taatagttta tttagtctcc tttctttttt
     1921 acagaaagaa taaagctaaa atagcttgta tcttgataac atttatttta atcttatagt
     1981 aatggattta ctacttacat ttttgataga atatttaaat aaaatcattc acgccaattc
     2041 aaatatcatg tataatgaat aaagttacga acttgatagt aaggaggagt tgttttgtca
     2101 ggaaacaaac aacacccaag taaagataaa aaagaagcca gtaaggcagc tacagcagat
     2161 ataatcgaca agcggagaaa tctgcgtaaa aaagaagata agattgtgag gaagatcatt
     2221 ttagtgattg ctcttacatt attgattatt ggcggatttc ttggatttac cgtgtatcgt
     2281 tatgtagata gcgggttgaa gcctttagat aaatcagatg accaattagt ccaagtcgag
     2341 atcccgagtg gctcttctaa caagcagatt ggtgagattt tggagaagga caatattatt
     2401 aaaagcggga ttgttttcaa ttattataca aaatttaaaa atttaactgg ctttcaagct
     2461 ggctattatc agttagcgcc taatatgact cttgacgaga ttggcaaaca gttgcaagaa
     2521 ggcggtactt ctgagccaac aaaagttgca gatgggaaaa ttgccattcc agaagggtac
     2581 gatatcgatc agattgctga acgggtagcg aaggtaactg gtaaagacaa gaaagaattt
     2641 ttagatttag tgaatgatga gactttcttt aacgagttgc accaaaaatt cccagaactg
     2701 ttggaaagtg catctaaagc tgagaacaca aaataccgtt tggaaggtta cttattccca
     2761 gccacatatg attattacac gagtacttcg cttaaggatc tagtgattga aatggtgaac
     2821 aagacgaata cggtcatgca aaactattat tcagctatca agcagaaaaa tttgactgta
     2881 caagaagttt taactctagc ttctctagtt gaaaaagaag gcgtaaaaga aaatgatcgt
     2941 aaaaacattg cacaagtctt ctttaaccga atcaaagcaa atatgccact tcagtcagat
     3001 atctctgttt tatacgcatt aggtgaacac aaagaattag tgacgtatga agatacagct
     3061 gtagattcac catataattt gtataccaat accggctatg gacctggacc ttttgacaat
     3121 ccaagcgaag aggcaatcaa ggcagtatta gaaccagcag aaaatgacta ttattatttc
     3181 gtagcagata cgagcactgg aaatgtttat ttcgctaaaa cttatgaaga acatatggaa
     3241 ttagtggaaa aatatgtaaa taattcttag aaaaacaggc tctagacatt gatcattttt
     3301 agaaaaacat ttacatttga agaaaagcgt ggtaatcttt tttggactat attcctcaaa
     3361 agattacctt ttgcggtatt ttatcgaaaa aaggagacgt tacacatggt agagaaagtt
     3421 tatccaatga ctctagaagg taaagaaaaa ttagaaaaag aattagaaga actaaaaaca
     3481 gtcaaacgcg gagaaatcat tgaacgaatc aaaattgccc gcggtttcgg agatctttcc
     3541 gaaaactctg aatatgaatc agcgaaagat gaacaagcat ttgttgaagg aagaatcaca
     3601 acaattgaaa atatgattcg ttttgctcaa atcatcgata acgacggtgt agatgcagat
     3661 gaggtatcaa ttggtaaaac agttacattc atcgaactac cagatggaga agaagaagag
     3721 tacacgattg tcggaagcgc tgaagcagat ccgttttctg gcaaaatttc caatgattct
     3781 cctattgcgc aagcattgat tggcaaacgt aaaaatgatg aagtaactat tgctactcca
     3841 ggtggcgata tgttagttaa aatcgtcaaa gtagaacaag cgtagtctga ctgaaagatc
     3901 tagagagttc atcttactga tgatacactc tagatctttt tctctttgtg tattttagat
     3961 ttcaacaaac gatcttacat ctaaagtcct atagccaaca gttaaaaaaa taggtatgat
     4021 gaaatgagaa tgtgtcagga gggagaaaga gcgatgaata gttcttggcg tttttttgtg
     4081 gtagttgaag cactactgct gttatttgct gtttggcaaa tcgtaaacaa tactggactt
     4141 ttgcttttag tgatattcgg tattttcaat atatatctag ctatgcgcaa acatcctcgt
     4201 actaaatttc aaaattttca attgatatta ggaagtttgg tcattttttt tagcttagtc
     4261 aacagtcctg ctttatggat gatggctgta tttgccgttt tatttattgg tctgaaaggt
     4321 gtagagattt cagggataga tttaaccaaa aatgcttttt ggcgaaagaa acagatcatg
     4381 atgatccaaa cagagcaatt aaaaacacat aataatgagc ggaaaagaca gcaattattc
     4441 ggtaaccaac gtattggaaa cgacgtctat gaatgggacg atatcaacat cgccgtcatt
     4501 tctggtgata ctatcataga tttgggcaat acgcttttgc caaaagatga taatattgtg
     4561 attgtaagaa aagggatagg cagaacacgg atcttagtac ctttgggcgt agccatccga
     4621 ttagagcatg cgacattagt aggaaatgtc ttgtttgaag aagagcaatt ttctttgaaa
     4681 aatgaacaaa tcaaaatcta cagcagtgat tatgatgaaa accctcgtag attgaagatt
     4741 attactaata ccttgattgg agatgtcgag gtgatacgaa tatgatggga aaaatatcca
     4801 gagcgatgct agctgtttat tcggggattg cggctttcct tattattcta ttttcacttt
     4861 tcacttattt ttatgccagc aatcaaagtc attggtgggg agaattactg cgtgcacgtt
     4921 tattatatgt tccgcttatc ttccatttgt tagccatttc cttaggtgta ggattaattg
     4981 tctttctatt attatcactt attcaaaaga caaaatacgg gaaaatcgaa gaaaagctgc
     5041 gtgcactttc ttctggcaat tatgaatcca aattgttgct tcttccgatc ccaagtgcat
     5101 cggacgattt atacatcaaa gatattgata aggaaatcac taagataaaa gaaaaaatga
     5161 ttgaagtatc tagtgaatta caaattgtaa cgagccgtcc ccaatatgta gatggacaaa
     5221 ccaaagaaga aattttagaa ttagaaagac atcgattagc ccgtgagctg catgattcag
     5281 tttcgcaaca attgtttgca gcaatgatga tgatgtcggc attgacggag caagcagaaa
     5341 aaagcgagac accagaaatg tttcgcaagc agttgaaaat ggtagcagag atcatcaacg
     5401 cttcccagtc tgagatgcgt gcgctgcttc ttcatctacg tccagtcaat ttagaagaga
     5461 aaagtttaaa gcaaggtatc gagcagttat tgaaggaatt gcaaaataaa atccagattt
     5521 cgctgaaatg ggatgtagaa gatgtaaaac tatctagttc cattgaggat cacttgttcc
     5581 gaatcgttca agaattgtta tcaaatacat taagacatgc taaagccaat gaattagagg
     5641 tatatttgca caaaatagac aataatcttt tgttacgtat tattgacgac ggaacagggt
     5701 ttaatatgaa tgaaacgaaa acgggaagtt atggattgaa caatatcaaa gaacgagtag
     5761 ctggtattgg tggaacagta aaaatcatta gttttaaagg tcaaggtaca agtgtagaaa
     5821 tcaaggtccc tttgatgaag gaggcataga taaggatgat aaaagtttta ttagtagatg
     5881 accatgaaat ggtgcgctta ggcgtctctt tttatttatc tattcaagaa gacattgaag
     5941 taattggaga agctgaaaat ggacgacaag gctatgagaa ggcgatgaca cttcgaccgg
     6001 acgtcatttt aatggactta gtcatggaag aaatggatgg tatcgaatca acaaaagcaa
     6061 tcttaaaaga ttggccagaa gctaaaatca ttatcgtaac cagttttatt gatgacgaaa
     6121 aagtttatcc tgccattgaa gcaggtgcag cagggtattt gttgaagacc tctaccgcac
     6181 atgaaattgc tgatgctata agggcaacgc aacgtggcga gcgagtatta gaaccagaag
     6241 tgacaacaaa aatgatggaa aaaatgagtc ggcgaaatga gcctgtatta catgaagaac
     6301 taacgaatcg ggaaaatgaa attttaatgc tgatttctga aggaaaaagt aatcaggaaa
     6361 tagcggacga attgtttatt acactgaaaa cagtaaaaac acatgtttcc aatatattgg
     6421 ctaagctaga agtggaagac cgtacacaag ccgctatcta tgcattcaaa catggtttgg
     6481 tgaaataaaa acgatgataa agaggataat caaaagctga gaaaataact attatttttc
     6541 ttggcttttt tacaataatg atgcttgctt ttgtttttgt aaaggctttg ttatactgag
     6601 aaaagaagat actaaaggag gaaaacggtg aaacaaaatt ttgcaattgt aggtcttgga
     6661 cgatttggcg gaagtatctg ccgaacactg atcgaagctg gtcaagaagt actggctatt
     6721 gatagtaatg aagatcgggt caatgaatat atgaatatag ccactcatgc tgttgtggca
     6781 aatgcacagg atgaaatgac acttcgatca ttaggcataa gaaatttcga tcatgtaatt
     6841 gtcgctattg gagaagatat tcaagccagt atcttagtta ctctgatggt aaaggaaatg
     6901 ggcgtaccaa atattttagc aaaagcccaa aatgagtatc atgccagagt acttgaaaag
     6961 atcggagctg atcgcgtcgt ccatcccgag cgagacatgg gaataaggat tgcgcataat
     7021 ttagtgtcaa agaatatttt agattaccta gagttgtcag ataaatattc tttagcagaa
     7081 atccgagtct ccaatcctaa atttttcaac aaaacattag ctgaattaaa tttcaggcaa
     7141 cgttttgact taacagtagt cgccatacga agagtagatc aaacggttat cgcttctcct
     7201 gcagcagatg aatatgttcg tgaaaatgat aatctactgg ttattggaga gacaggcgat
     7261 gtggatattc tagacgataa gatgaatcaa tagaaagaaa agagaaagta ggttgttggg
     7321 atgaaattaa cggtaacgcc aaaagcacaa aagtggtttg actcagaaat tgatctgcca
     7381 gaaacttacg gaatacgttt ttttggaaaa gtctacggca aaacagaggt tcatgatggt
     7441 ttttcgattg gaatgtcagt tgaacagcca gaacatcctg taaagaaagt ggaaattgat
     7501 gggacacttt tttttatcga ggaagcagat gaatggttct ttaaaggata tgatttactt
     7561 gttgattatg atcccaacct ggaggagcca acctatcact ttaaaaaaca ataaaaaaag
     7621 agagggtgac acaatttttg tcaccttctc ttttttcttt tgaaaatatt gttttcagct
     7681 attaatatcc tgcagacaat tttacttgat tggtttgaag ctcatgggtg gcaaagtagc
     7741 ttttcagatt ggttaaaaaa atagccatta atttcttttg aaaatcaggt gtcaaacctg
     7801 caatgtggga agtaatcagt acattgtcca ttttccaaag aggatgatct tgtggaagag
     7861 gctcttcctc aaaaacgtct aatgctgcaa aggaaagttg ctggttattt aaagcctgca
     7921 ccagatcttg tgtcttaaca gaagcaccac ggccaacatt gatgaataca gattttggat
     7981 caaagtgttc aaacatatgc tgatcaaaga gcccagttgt ttcctccgtt agaggtaaga
     8041 tattgactac gaaatctact tcaggaagaa cttcctttag ttgatttaaa gaaaaagtct
     8101 tttccattcc ttctgctgga tgaccggatg tattgattcc atagcagttc atgtctaatg
     8161 ctttgcattt ttttgcaatc atttttccaa tatggccagt tccaatgatt agtatttttt
     8221 tcccttggat agattggtaa tggaccgatg aacctaacca tttcatgtca ctctgtgcct
     8281 tttgtgcttg aaaaagacca cgagaatacc caaggatgat tcctaagaca tgctccgtaa
     8341 tcgattcact gtggatgcca ctaccatttg aaagtaaaat atgtttcttt gaaaaagtct
     8401 caaggggtag ctgatcaaca ccagctgaga tagattggac ccatttcaag gtagaagagg
     8461 ctaatagttt ttcttggtag tctgagttcc agcccaaact gatctctaca ttttgtagat
     8521 cctctggttg taattctgtt ttaaagatat aaccaggcgc tatagtatgg atttcttcga
     8581 taaattcatc acgaaatgga cgatctgcat aaatgattct ttctgacata gttaatttcc
     8641 cgcctttctt tactctaagt gtagcgcata caagaagaac tttgaagcgc cttgcttgtt
     8701 ttttgagaaa gaaatatttt tcaaataaaa aaggggctgt gacaaaaaaa tgtcacaacc
     8761 tctaaaaggg tctattgaag tagaaataac gagttgtctt tatttcttaa ttgatcgata
     8821 tttttgccca tcctttgaca gcttgagcaa aaataaagat tcctgcaaaa gcaatgaagg
     8881 cgctagtgtc aaaagaagga agaaagacca tcaagattgt tgcaattata gtaggcaatg
     8941 aaattgaata gatcaatgtt tttaaacaat caagaaaagt agcttttgtt agtcgaaagc
     9001 gtgcatagag aaaagcagca atgttggtaa atagcaaagt gatcagcaga ttcagaaaac
     9061 taggataaat agaaaatata aatgtcagga cttttatcca aagtggtata gatgcttcac
     9121 ttaaaaaaga acggagctgc ttacctgtta agttttttaa agattcattg gtgtagggaa
     9181 gttctaattg gttattgttc aacaaagcag aagtcgtgcc tgtattagga agagctatga
     9241 taagtttgtt ttttaacatc ccgacactaa caaaattccc catcaaatca gtagagatgt
     9301 cctgttctgt tcgttttccc tctggatcaa atgtgaagat aatggagttt gtttggtaaa
     9361 tgaatccttc tttttcttcg gtttgaagct gcccgttttg gattgtaaaa tcaggaatgc
     9421 gagtagcaat cttttgccca tccaattgaa tgttgtttaa gacttgaaaa acttgaaaag
     9481 agattggcaa agccataatt atactcaaaa ctaaaagata gacaatggct ttccaaaatg
     9541 ggacatttct cgcctccttt aggtctttga atcgtataaa ggaactgata attaattgtt
     9601 ttgttgtcat aagtactcct catctagtag tgtcatcact tgcaattcta gcgaagtatc
     9661 aagtgaaata caagtgcttt aaggaaacag ctgaatgatt ggtgaaatat caaaaaaatg
     9721 ttattcttgt ctaaagacta tccaatataa ataaaatgga ggaaggaata gtgcctacac
     9781 agccacagag atcatcaaaa aaactatttg gagtagtggt tttgaatgcg gtgatcactt
     9841 tatcagaatt tattggaggg attatgtcag ggagtttgtc tcttgtttca gatgctttcc
     9901 ataaccttag tgatacattg gcaattattt tttcttacgg ggcacaaaaa atggcccgaa
     9961 aagaagcaaa taaaaaacgt acgtatggct accagcgttt agaaatattg agtgctttca
    10021 tcaactcatt catattgatc atattatcct tgtttttaac cgttgaagca ttcaagcggt
    10081 tcacttcacc agaaaagata aatagtcatt tgatgctaac agtagcagtt attggattgc
    10141 tcgcgaattt gttctcagta ttattattac gacaagaagc agatgaaagt ttgaatataa
    10201 aatctagtta tttgcatttg ttaagtgata cactgtcttc ggtaagtgtg attatcggag
    10261 caattttgat ccgttttttc gatatctact ggatcgatcc agttattaca ctgatcattt
    10321 cgatatatat attgatagaa gcgataatgg tgatcaaaaa agctgccgct attttgatac
    10381 aaagtgcacc tgctatcgac tatgaaaaaa tggaacaaga aattgaagca atcgatgggg
    10441 taaaggatgt tcatcatgtc catatttggc agtatagcga aaagataatt attttcgacg
    10501 gacatattga ttttgaagat cagctactaa gcgagattga aaagatatat cctcgtataa
    10561 tttctttatt aaaattaaaa tatggtatta cacatgtgac gatccaagca gagacaaagg
    10621 caaaggatca aaaaaaatta atttttttaa ataaagatga aagttattga aatatagagt
    10681 atgataaata agaattttta ataaggtagg ttggctcatg aagcagtatc gacagttaaa
    10741 aggatattta gaagcaaaag gatattggga gatactagtc tatctctttt ttggtggttt
    10801 ggctacgatt gtaaattttg ttagttttgc tctcgcacgc caatattttg atttaagtat
    10861 ggccttatcc aattctattt cgtggttttg ttctgtattg tttgcttttg taacgaacaa
    10921 actttgggta ttccattcaa aatcaccgaa ttttacacat gcacttattg aatgcggtaa
    10981 gttcttcttc taccgtatcc tttcttacgg attggacatg ggggcaatgg tattgttgat
    11041 caatgttatg aacagcaatg agtatgtggc gaaaatcatc actcaaatta ttgttatcct
    11101 tgcgaactat attttcagca agttgtttat ttttaaagaa actgaggttt ttgaagaaga
    11161 agtaggtcaa agtgaaaaag acgactaggc ataacaattg aagaatttcc ttctctttgt
    11221 tactctttgt aatgtagaca ttattaagaa aacattgagg aggaattttt ttatgactta
    11281 cacattacca gatctacctt atgcatatga cgcattggaa ccttatattg acgaagaaac
    11341 gatgcatctg catcatgata agcatcacaa tacttatgtg acgaatttaa attcagcaat
    11401 tgagaaatac ccagaattag gcgaaaaaac aatagaagaa ttattatctg atatggacgc
    11461 tattccaaca gatatcaaga cagctgtacg taacaatggt ggcggacatg ctaaccattc
    11521 atttttctgg gaaatcatgg caccaaatgc tggtggcgaa cctacaggag aaataaaaga
    11581 agcgattaat gaagcttttg gtgatttttc ttcttttaaa gaagaattca aaaaagcagc
    11641 cgctggacga tttggttctg gatgggcttg gcttgtaatg gaaaatggaa aattagctat
    11701 tacctctact gcaaatcaag attctccatt gatggaagga aagacaccaa ttctaggttt
    11761 ggacgtttgg gaacatgctt attatttgaa atataaaaat gtccgaccag attatattgc
    11821 agcattctgg aatgttatta attgggatga agtaaacaaa cgtttcgaag cagcaaaata
    11881 accatttcct ccgtcatatc agtcttaaaa ataatttgga aaataaaaga gcgaaagctt
    11941 gctagcgttt tctaaattgg gtataatgtt cttgcaaggt tacttgcaag aacttttttg
    12001 gaggatatac atttatggaa cacggaacgg ttaaatggtt caacaatgaa aaaggatttg
    12061 ggtttattac agtagacggc ggagatgacg tatttgttca tttctcagca attcaaggag
    12121 acggcttcaa gagcctagaa gaaggtcaag aagtagaatt cacgattgtt gaaggtgcac
    12181 gcggacctca agctgctgaa gtgactaaat tataataatt tgttgaataa agac
//
LOCUS       DAYYKN010000084        12144 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000084 DAYYKN010000000
VERSION     DAYYKN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.084"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..566
                     /locus_tag="ACHBB7_001948"
     CDS             <1..566
                     /locus_tag="ACHBB7_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM9209893.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /gene="fic"
                     /locus_tag="ACHBB7_001949"
     CDS             967..1572
                     /gene="fic"
                     /locus_tag="ACHBB7_001949"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM9209894.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="ACHBB7_001950"
     CDS             1588..2160
                     /locus_tag="ACHBB7_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9209895.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="ACHBB7_001951"
     CDS             complement(2412..2669)
                     /locus_tag="ACHBB7_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209896.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="ACHBB7_001952"
     CDS             complement(2795..3610)
                     /locus_tag="ACHBB7_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9209897.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="ACHBB7_001953"
     CDS             4127..5452
                     /locus_tag="ACHBB7_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM9209898.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="ACHBB7_001954"
     CDS             5445..5795
                     /locus_tag="ACHBB7_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209899.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5792..5962
                     /locus_tag="ACHBB7_001955"
     CDS             5792..5962
                     /locus_tag="ACHBB7_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209900.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACHBB7_001956"
     CDS             complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACHBB7_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM9209901.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7113..8066)
                     /locus_tag="ACHBB7_001957"
     CDS             complement(7113..8066)
                     /locus_tag="ACHBB7_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9209902.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8678..10171
                     /locus_tag="ACHBB7_001958"
     CDS             8678..10171
                     /locus_tag="ACHBB7_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM9209903.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10306..10602
                     /locus_tag="ACHBB7_001959"
     CDS             10306..10602
                     /locus_tag="ACHBB7_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM9209904.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10703..11554
                     /locus_tag="ACHBB7_001960"
     CDS             10703..11554
                     /locus_tag="ACHBB7_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209905.1"
                     /translation="MKSDDWIEHNWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11575..11787
                     /locus_tag="ACHBB7_001961"
     CDS             11575..11787
                     /locus_tag="ACHBB7_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209906.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4172 a         1863 c         2268 g         3841 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa atatatttag
     6721 tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca
     6781 tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta aatatattta
     6841 ctatatctag tatacttact atattttaat cttttgatcg tctattatat ataatccgtt
     6901 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
     6961 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
     7021 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
     7081 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
     7141 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
     7201 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
     7261 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
     7321 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
     7381 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
     7441 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
     7501 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
     7561 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
     7621 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
     7681 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
     7741 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
     7801 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
     7861 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     7921 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     7981 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     8041 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattta
     8101 gtatacttca tatacttaat atattcgata tatctaatat actccaaatt gataagaatt
     8161 cagactttat aaggaatata gtggatatac taaatatatt tgctatattc agtatatcag
     8221 atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta gagtgaaaag
     8281 aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt agcaaaagca
     8341 gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa ttgattttag
     8401 cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg tctcaagaat
     8461 atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt ccgattgcct
     8521 ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag tgtacttaaa
     8581 ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta aagctaaact
     8641 gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc
     8701 tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga aacctgcagg
     8761 tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa
     8821 tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg aaaagaaact
     8881 aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt atcgaacagt
     8941 ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag ttgatatcga
     9001 ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag tgggctacat
     9061 gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac taaagacacc
     9121 aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa
     9181 aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat gtaaccactt
     9241 cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga actatcagta
     9301 ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga
     9361 agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga
     9421 accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg
     9481 tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt atgcattgga
     9541 tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc tatctgaagc
     9601 agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag caagcagaga
     9661 attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt
     9721 cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa aatatagtca
     9781 taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc ttgctagtga
     9841 gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac
     9901 acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca aggtaacacg
     9961 aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga gggttattca
    10021 actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat ttggactcaa
    10081 cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc ctcttaaaga
    10141 agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt gccttataca
    10201 atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg taggtggtat
    10261 acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc gttaaaaaat
    10321 tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt atctgtggaa
    10381 gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt taccagggaa
    10441 aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc ttctggggag
    10501 actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat tgagtataaa
    10561 atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt atttacttaa
    10621 aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac gttaaaggaa
    10681 agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca actggcttcc
    10741 tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc cattaggaaa
    10801 actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga ttggagtatt
    10861 tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag acatttttac
    10921 agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat tagtcatgca
    10981 atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg atttaattgt
    11041 tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa gaacaacaaa
    11101 aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca tgtatttgtt
    11161 tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta ttaaacgttc
    11221 aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg agtacaatgt
    11281 tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga gtatccatga
    11341 atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga aagcaataga
    11401 tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat atggcatcgg
    11461 aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt tttggaacag
    11521 tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt gaaaatgatt
    11581 cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa gaaggagcag
    11641 ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg tacgatgaac
    11701 attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gttgattcga
    11761 aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt gatgaatggg
    11821 aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt gtaaagagca
    11881 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
    11941 tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag gatagtgaag
    12001 gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg ctatcctttt
    12061 taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa aatcgtaaga
    12121 gaaattgatc gaagatgtga cgaa
//
LOCUS       DAYYKN010000085        12009 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000085 DAYYKN010000000
VERSION     DAYYKN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.085"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..2048
                     /locus_tag="ACHBB7_001962"
     CDS             30..2048
                     /locus_tag="ACHBB7_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9209907.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2118..2324
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2475..4889
                     /gene="leuS"
                     /locus_tag="ACHBB7_001963"
     CDS             2475..4889
                     /gene="leuS"
                     /locus_tag="ACHBB7_001963"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM9209908.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5237..6790
                     /locus_tag="ACHBB7_001964"
     CDS             5237..6790
                     /locus_tag="ACHBB7_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM9209909.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7171..8955)
                     /locus_tag="ACHBB7_001965"
     CDS             complement(7171..8955)
                     /locus_tag="ACHBB7_001965"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209910.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8970..10691)
                     /locus_tag="ACHBB7_001966"
     CDS             complement(8970..10691)
                     /locus_tag="ACHBB7_001966"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209911.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10791..11552
                     /locus_tag="ACHBB7_001967"
     CDS             10791..11552
                     /locus_tag="ACHBB7_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9209912.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3935 a         2173 c         2432 g         3469 t
ORIGIN      
        1 atagataaat tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa gaacggagac
       61 gtttagaaca gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc gtgggaactg
      121 cgatggtagg ctgttctgtc gctgccccat tagtacagcc ggttcctgtt caagcggatg
      181 aaacacctgt tcagttcggc gcaagaatca atacagcggc atttatcaca gaaattgcta
      241 cttatgctca accaatcgct caagccaacg atctatatgc atcagtgatg atcgcacaag
      301 cagttgtaga aagcggttgg ggcagtagtg cgttatctca agcaccttat tataatcttt
      361 ttgggataaa aggcagctat caaggacaaa ccgtctacat ggatacgctt gaatttttaa
      421 ataatcaatg ggtaaataaa aaggagcctt tccgacaata tccttctttt gctgaatctt
      481 tcaatgacaa tgcctatgta cttagaaata cttcatttgg caacggttat tactatgccg
      541 gtacttggaa gagcaacacg aaatcctata cagatgcgac agcttgtttg acagggcgtt
      601 atgcgactga tcctggttac gcaggcaaat taaataatat catcgcaact tacggattga
      661 cccaatatga tacaccagct agcggaaacg caggcggtgg agcgactaca ggtggcaata
      721 atactggaag tgcaggtagt actggcggtt caagcaattc tggtaataca aactcaggtt
      781 cagcaacttc gaccacttat actgtccaat ctggtgattc tgtttgggga atctcaaata
      841 aattcggtat cacaatggat caattgatcc aatggaacaa catccaaaac aatttcattt
      901 atccaggaca aaaactgaca atcaaaggta gccaggaaaa tggctcttcg acgaataatt
      961 ccggtaacaa taccaactct tcaggaaatg ctggtacatc aaacggagga caaacaactg
     1021 gtgcgaaata cactgttcaa tctggtgatt ctgtttggaa gatctcaaat gatcacggaa
     1081 ttacgatggc tcaattgatt gaatggaaca atatcaagaa caatttcgtt tatccaggac
     1141 aacagctaat cgtatcaaat ggcggttctt ctaatgctgg agcaaatact ggctcaaata
     1201 ctgggaatac aagtggctct agttcgtcca gctcaagttc ttctaataca gcttcaggaa
     1261 cgaaatatac agttaaagcc ggagactctg tttggagcgt ctcaaataaa tacggaatta
     1321 cgatggctca gctaatccaa tggaacaata tccaaaataa ctttatctac ccaggacaac
     1381 aactgactgt ttcaaacagt ggatcaacaa acagcacgtc aggtaataca ggttcgtcaa
     1441 ccaatacaaa taacagcagt tcagttaata ctggcagcag ttcgtctcaa ggcacttata
     1501 ccgtgaaagc aggtgaatct gtttggagcg tggcaaataa aaacggcatc acgatggatc
     1561 agttaatcga atggaataat atcaaaaaca acttcattta tccaggacag caattgatcg
     1621 ttaaaggtgg aacttcggca aatacaaata caggttcaac tacttctgca gcgaaaccaa
     1681 atacaccgaa tacgtctgct acgacaagta catcatccac tggtaatacg atgtataccg
     1741 tgaaagcggg cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg atggatcagt
     1801 taatcgaatg gaataatatc aaaaataatt tcatctatcc aggacagcaa gtgattgtga
     1861 agaaaggttc atctcaatca acaaaccaac aatcagttac tggttcgaaa acctatactg
     1921 tgaaatctgg agaatctgtt tggagtgttg ctgacagtca tggaattaca atggctcaac
     1981 taatcgaatg gaacaatatt aagaacaact ttatttatcc aggtcaaacg ttgattgtag
     2041 ctaaataaaa tagctgttcg aagaaataac gattgaattc cacaaaaata tttgttacta
     2101 taaggctact taaatatgac aacacttgaa aaggaagtag taaaaagact gaagtttcag
     2161 agagcgcatg gctggtgaga atgtgcacgg atgcttttga actcgccttt gagttgccct
     2221 tgctgaatac ccgagtaagc aagcggcggt gacggccgtt acaacgttcg aggccttttc
     2281 actaaggtaa aattaggtgg taccgcgtag attacgtcct ataaaaagag caatcttttt
     2341 ataggatttt tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg aaataaaaat
     2401 aaacagattc tgccggtttt ttcgttatat ttataataga aacaagtgtt ttcaatagaa
     2461 caggaggaca gcaaatgagt tacaatcata aagaaattga gaaaaaatgg caaaaatact
     2521 gggcaaaaac gaatactttc aacacccatg atgatcctga aaagccaaaa ttttacgcac
     2581 tggatatgtt tccctatcca tccggtcaag ggctacatgt agggcatcct gaaggttata
     2641 cggcaacgga tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg ctgcatccaa
     2701 tggggtggga tgcattcggt ctgcctgctg aacaatatgc gctagataca ggaaacgatc
     2761 cagctgagtt tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat tctttaggat
     2821 ttagttatga ctggaatcgc gaagtgaata caactgatcc ggagtattat aaatggacac
     2881 aatggatctt tacgaagctt tatgaaaaag gtctagctta tgaagcagaa gtagcggtaa
     2941 actgggtacc tgaattagga acagttattt caaatgaaga agtgatcgat gggaaaagtg
     3001 aacgtggtgg ctatgatgta gtcagaaaac caatgcgcca atggatgctg aaaatcactg
     3061 cttatgctga tcgcttattg gatgatctag aattagttga ttggccagaa agcattaaag
     3121 aaatgcaaag aaattggatc ggtcgttcag taggagcaaa tattgaattt aaagtggctg
     3181 gtacggataa atcctatacc gtatttacga cacgaccaga tactctattc ggtgcaactt
     3241 actcggttct tgcacctgaa ctagatcttg ttagagaaat tacgacccct gaacaaaaag
     3301 ctgctgtaga agcttatatc gaagagacag ctaaaaaatc agatctaaag agaacggatc
     3361 ttgcaaaaga aaaaacagga gtatttactg gtgcttatgc aatcaatcca gttaatggca
     3421 aggaaatccc aatctggatc gccgattatg tattatcttc atacggcaca ggagccatca
     3481 tggctgttcc agcacatgat gaacgtgact ttgaattcgc tcaaactttc ggactagaaa
     3541 ttcttccagt tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa gatggtacgc
     3601 acatcaactc tgaatttcta gacggcatga acaaacaaga agcaatcgat aaaatgaatg
     3661 aatggctaga agaaaatggc gtagggaaaa aagaagtcag ctatcgctta cgtgactggc
     3721 tattctcccg tcaacgttac tggggtgaac caatcccaat catccattgg gaggacggaa
     3781 cagtcacagc tgtcccagaa gaagagctac cattacggtt gcctaagaca aaaaacatca
     3841 agccaagcgg tactggagaa tctccattag caaatatcga ggagtgggtc aatgtcgttg
     3901 atcctgtaac aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa tgggcaggaa
     3961 gctcatggta ttacctgcgt tatatcgacc ctcataacaa aaaagaacta gcagattatg
     4021 aaaagttgaa gcgttggcta ccagttgaca tctacattgg tggagcagaa catgcggtac
     4081 ttcatttact atatgcacga ttctggcata aattcttata cgatatcggt gttgttccta
     4141 caaaagaacc attccaaaaa ctatacaatc aaggaatgat cttaggcgaa aacaacgaaa
     4201 aaatgtcaaa atctcgtggt aatgtagtca atcctgatga tgtggtcgaa acttacggtg
     4261 cagatacact gcgcatgtac gaaatgttca tggggccatt agatgcttcg attgcttgga
     4321 gcgaaaatgg cttagaagga agccgtaaat tcttggatcg tgtatggcgt ttgatcgttg
     4381 atgagaacaa taaaatgcgt gaccgtatca ctacattgaa tgatggcaag cttgataaag
     4441 tgtatcatca aacagtgaaa aaagtgacag aagactatga aaatcttcat ttcaatacag
     4501 caatctccca attgatggtc tttatcaatg aagcctataa agtggacgct ctgccttatg
     4561 aatatatcga aggatttgtt caactactcg caccgattgc tcctcacatg ggtgaagaac
     4621 tatgggcaat ccttggaaat gatggtggta tttcttacgc accatggcca acatacgatg
     4681 aagcagcact tgttgaagac gaagtagaag ttgttttcca agtaaacggt aaagttcgtg
     4741 cgaaaagcaa tgttccccga gacttaggaa aagatgagct agaaaaagca gcattagcca
     4801 atgaaatagt gcaagaatat attgaaggca aaacagtcag aaaagttatt gcggtaccaa
     4861 acaagttagt caatatcgtt gctaattaat tggctctttg tcaataagga ctgatgagtt
     4921 gtgcaaaatc aaatctgggc cagaatgaac ttcattctgt ctcagatttt ttgttatttc
     4981 aaattgatta attgattgat caaaaaggtt ttgattttca tgttctcaaa cacgttcaat
     5041 gtctttttct tctgaatggt cttgtaagag atctctgtaa tcatcaaatt tttgtctatt
     5101 aatcttagta ttttttgatg tataaactac ctgaaattga gagtacatac caaattcata
     5161 aattttttat tagtgggact agtgattgta tggattttga cgcataatga atgaagtatg
     5221 agaaaaggag agacaaatgg atcaagaact atttattatc gaagtattat cagctaaaca
     5281 aaaaattatc gagaaagcga aaaaaagagc gaaaagaata atgttcaagc ttttaagctt
     5341 agtatttttc tctggtttgc tttttttcgg agctgtatcg ttcctatatc gccatatgag
     5401 tgcagtagtc cgttcagctg aagggatgca aaaattaaat aatttgtatc acttttcgtg
     5461 gggagttggc ggaataacaa ttgttgtgct tattgttttt cttctactta tttggctccg
     5521 ttaccatcaa ttgtcgcgtc atttcgatca atggcttagt caaagagcac aacagttttt
     5581 acataataaa ccaacttctc aagatgatga ctatctttat tttgagaaga cttcgacgag
     5641 tcaagctttt cggatacaaa aaagtgattg ccagttattg ctgagatcac ttgatgattt
     5701 acctatctac ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc atattttttt
     5761 actagaaaac gaacccagat gggaaaaaag ccagtcttcg cctggtggat acctgacaac
     5821 taagatgagt acattagtca ctggtttttt cctcattctt ttagctggaa caatctatta
     5881 tgggagatac actcagccaa aaatgtcttc tcatataaat gctgcttctc acatgacaat
     5941 gaactcaact aacgatacgc atatacaaaa gacaaatgaa gggttgttag ctgatcctaa
     6001 gcaaaaggta aatttgacaa cacaaaagat aaatgatctc aaacttgata cagagacgca
     6061 ctctctctat atgacaacag atagtggtgc taattgggta tttgtgccga ttgattcgca
     6121 atggttgcgt tttggcgaat acaccctgac aacggggctg attcctgaag gttattggat
     6181 ggacaacaca tatgatattt ccgccgattt ctcatggttt atctattctt ctggggatga
     6241 tgtacgtctg ctgacttcaa gagataatgg taaaacatgg caaacgtcta ttatcaccca
     6301 atcagttcag ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg gtctatttat
     6361 ctgtacatca gccagtggga tgtctgcaga aactttgtac atttacacta ctatggatgg
     6421 cggaatcact tggataaaaa gtggaagtac aatgatcaat cagccagtac aaaatgtcag
     6481 cttcgttaca acttctttag gttttgtttc gacacgtgaa agtttgtttt atacaaacaa
     6541 cggaggacgc tcattcaaag aatcagttct gtcgattccg gaaaactatc agacaggggg
     6601 gatagatctt tttcaatcac caaatgaagt tactcaaata agcgcgaata cgttagagac
     6661 aaaattttat ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg cctgcctctt
     6721 ccgttcaact gataacgggg aaacatggca attcgttcag caactatcgc aagtagaaca
     6781 aggagattaa agaagagaga ttgtgagcct gacgttcagc accgagtcac aagaaacaaa
     6841 ttcaaaaaac agcttctcat atttgttgaa tagtgtaact cgtgattcgg agttgacaag
     6901 agcaacaaga agcgattttg aagtatagct tctcgtattt tacaagatcg tgtcttattg
     6961 ccgataggtt gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac aagtcttttg
     7021 tcactactta ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag tattattctt
     7081 tgtgtttcta gtggcaccac tagtacattt aaaaaagcta tcgatcatgg actaatcata
     7141 catgatcgat agctttttag ataatgtttg ttacgctatc tgaaattgac tattataaag
     7201 actggcataa agcgttggtg attggagtag ttcagaatgt gttccttttt cgacgatatc
     7261 gccattcttc attacaagga tcatgtctgc attttcaatc gttgacaaac ggtgtgcaat
     7321 cacaaaactt gtcctatctt ttgtcacagc ttccattgct tgctgaatca atgcttctgt
     7381 tcgtgtatct acgctagaag ttgcttcatc taaaataacg acaggaggat tcgctagaat
     7441 gactcgagca attgtcaata attgttgctg tccttgagat aacgtgccgt tttcgctaga
     7501 aatgatcgta tcatatccgt ctggcagtgt tcgaataaag tgatcacatt gtgcgatttc
     7561 agccgcttcg ataatttgtt ctcttgtggc atccattttc ccgtaggcga tattatcagc
     7621 taccgttcct tcaaacagcc aggtattttg taaaaccatc ccaaaagaac gacgtagttc
     7681 tccacgtgaa agcttggtaa tatcaagacc gtcgaattcg atctttcctt tattcagttc
     7741 ataaaaacgc atcagcagat tgactaaagt cgtttttcct gcaccagttg gaccaacgat
     7801 cgctaccgtt tgttttggct ttactgaaaa atcgacttga ttcatcaata ttttatctga
     7861 tgtgtaacca aactgtacat ttttgaaagc tatttttcct ttcggttgtt ctaaaagtct
     7921 tgcatctgga aggtctggaa tttcatctgt ttcatctaat atctcaaata tcctatcgat
     7981 tgctgccatt gcggattgga tcgagttgat cacataagaa gcggtagaaa ttggttcaga
     8041 aatctgattg atgtactgaa gataagcttg taagaagccg atcgtcacac cacctgatag
     8101 aacaagcata gcaccgaata ccgcacttat gatgaaggcg agttgattaa tgaaacgaat
     8161 ggccggatag atagcacagt tcataaattg agcacgttta aaagcgttgt attgcttttt
     8221 atttacttcg tgtatagcag cttctgcatg agcttgctga ttgaatgttt tgaccactag
     8281 attaccagct aagatttctt ccattcgatt atttaaaaca cccagttcat actgattctg
     8341 ttcagaaatc tgtttgtttt tgttcgcaat cttagttgta acaattgaac tgccaacgat
     8401 cagcaataag acgagcaaag taagcttgct ttcgatataa aacagcatca gaccagcagc
     8461 agctattgtt acgacagaag taaaaaattg attgatacct gtcaataaca cttgagaaag
     8521 ttgattgaga ccggtagttg tccgactaat gatgtcacct acttgatgat tgtcaaaaaa
     8581 cgacatcggt aatgttttga atttttttgt gatttctttt cgcaccctca atgtgatctt
     8641 ttcgcttaac gctgccattg tccgttcctg aataaatgaa gtgatgctgc ttaagactga
     8701 acagccgatc agcaataaga cagggaacaa caatgcttgt ttgatatcac tgaaagaaag
     8761 ctgtccaata cctgtttttc ttatttctga taagagatca tcgatcccga ttcccatgat
     8821 caagggcatg gctacgacta ataggttccc gaataaactg catagaaaca atccgtaaaa
     8881 atatttatgt tcaggtttga tcattttcca aaaacgtatc aaagcacgcg tgttcaacgt
     8941 ttttttcttt tccatcgtat cccttctttc tatattcctt gtgattttgc aaactcctga
     9001 taagtttggc aattagacag tagttcttca tgagtacctt gtccaacgat ttttccttca
     9061 tctaaaacaa tgatgttatc tgcttgttgg attgtactga ggcgttgagc aacaatcacg
     9121 agagttttct ccgacatgtg tttatgaagg gctgctctta atgctgcatc tgttttatag
     9181 tctaaagcgg agaaactatc atcgaataca tagatctctg ctggtttgat caacgctcga
     9241 gcaatacata tccgctgttt ctggccccct gaataattag aaccgccttg agcaacaaag
     9301 ctgtccaatc cgtcaggaag ggtgtccact agttcttcta attgagcgat cttgatcgct
     9361 ttagacaact cttctgttga ggcatattca tttcccatct gcaagttgct taaaattgtc
     9421 ccgctgaata agaaagcctt ctgagggaca taactgatgt gggagcggat ctgttcttga
     9481 gtcatttgag taagaggaat accaccaagg gttacttttc ctatcgtagg gtcactaagt
     9541 ctcaagagaa gtttcgccaa actacttttt ccagatccgg tcccgccaac gatcgctgtt
     9601 gtttttcctt taggtattgt gaaatcaata tcttctagaa ctggatcaga gatttcagta
     9661 tatttaaatg tgacatgttc aaaagcagct gcatttgtct gatcaaaggt tggttcagct
     9721 actgttttga tcgtctcttc ctcaatttcg atctctgctt gcagtatttc ttctatccgt
     9781 cgtaatgagg caaacgattt agggataatg accaatacca tagctgcgat catcaagtaa
     9841 gcaagtgtca gcatagagta ttcgactaca gcagtcacga caccgatttg taatgctcct
     9901 tctgctacaa gatttccgcc aaaccacaaa atagaagcat aaacgattcc catgagcaaa
     9961 aaagcaattg gagtgaccca agcaaatatt cggttcactt tgatcatttg attagcatag
    10021 tcagcaaagc tcttattcgt tcgcttttct tcatatgatt gattatcaaa ggcacggatc
    10081 atattaatcc cagtaaagaa ttctctcaat gtgatggtga tccgatccat ttttggctgg
    10141 atactcaaag ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca tacataaaaa
    10201 acaacagaga tcaatgtaac ccatgttagt aatagagaat aacgaaacat catgagaatg
    10261 gtgaaaacgc aaataaccgg tgctggtaag atcatttggc aaaataaaac aatcatttgc
    10321 tggacattat cgacatcatt cgtcattctt gtcaaaagag atccagaact aaaacgactg
    10381 atatccttca ttgacagctg ttggaattta cggaaatatt tctcccgcgt ttggaatcca
    10441 aacctagcgg aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat tgttcctaga
    10501 aacgctacaa gcagcatttc aactccaatt ttttttataa ctgaggcttc gccacttgct
    10561 atacctgtgt cgatcagctt tgctactaat tttggtacac ctaatgtacc ggcgatttct
    10621 aaacagatca tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa gaatgttttt
    10681 aagatagaca tattattcct ccttttttcc tgatactttt cgattctata ccttcaagta
    10741 acttgaaggt caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa atgaaaaaaa
    10801 tgtatacgat tggccaagta tcggaactat ttgatttgcc aaaatcaacg atccgctatt
    10861 gggatgaaca aggattaatc cgttcaagta gacaagaaga gaatgattat cgattgtttg
    10921 atatcgatga tatttttatg ctttatgata tcgtttttta tagaaaatta gatataccga
    10981 tcaaacagat gaagaattta tatggaaaaa cattgacgga gttatatgag acacttgatg
    11041 aaacagaaag aagaatccat aaagaactgg tggtgatgaa acaaaaacaa aaagagataa
    11101 gggagcaaaa aaaacagttg aaattgatga tcgatacaaa cgaggaagaa tttcctgtag
    11161 aagagatccc ttttgactgt atgatctcaa cggagtttga agatattgtg gaaatcaaaa
    11221 aatttctacc taactattca agctttggga tgatgagtat gccagcttcc agttcacaga
    11281 cgatgtatgg attttttatc gaccctagtg aagtacattt atttactcaa gacgtgattt
    11341 gggagaaaaa agatacggct gtttatcgtc gattcttatt gaaatccgag atgaaccatg
    11401 cggaacgtaa taatatttta gaaatcagag aaagaatgta tgaaaaagga tggaaaacag
    11461 gagaagtgat cggccagtat ctattgacga atacggatga aaacaatatc agaactgaat
    11521 attatcatgc atggatagaa atgaaaaaat aggaaatatg tgtttaagat aactcgaaaa
    11581 tgatggttgt tttagttata aagaaaaatt ttctggttcg attacgggtt taatgtgggg
    11641 gattattagt cgtggataga tagaatttta gttttttaga ggaaatatgt ctttaaaaat
    11701 ggaatggttg caattcttag cgatatatac gatgtaattg tcctcttaaa aaagtatgga
    11761 aaaataattg tattgggtgt tatgtataat tcatggtgaa ctgagactaa tgagaagaaa
    11821 aattcatatt gttatgagtg agattgtatc agatgagaat gataaattaa ataggtgcat
    11881 aaaagcatta cacttaaaat acattgttgc ttacattatt acaatatgtt aataaggcaa
    11941 aaataaatag atggattgaa taaacatatt ttttaatcaa atagtcccta ctgtgacttt
    12001 cctcagcta
//
LOCUS       DAYYKN010000086        11828 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000086 DAYYKN010000000
VERSION     DAYYKN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.086"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(144..464)
                     /locus_tag="ACHBB7_001968"
     CDS             complement(144..464)
                     /locus_tag="ACHBB7_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM9209913.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(612..2846)
                     /locus_tag="ACHBB7_001969"
     CDS             complement(612..2846)
                     /locus_tag="ACHBB7_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9209914.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3325..3591
                     /locus_tag="ACHBB7_001970"
     CDS             3325..3591
                     /locus_tag="ACHBB7_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM9209915.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3591..5318
                     /gene="ptsP"
                     /locus_tag="ACHBB7_001971"
     CDS             3591..5318
                     /gene="ptsP"
                     /locus_tag="ACHBB7_001971"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM9209916.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            5482..6528
                     /locus_tag="ACHBB7_001972"
     CDS             5482..6528
                     /locus_tag="ACHBB7_001972"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9209917.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6545..7768
                     /locus_tag="ACHBB7_001973"
     CDS             6545..7768
                     /locus_tag="ACHBB7_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9209918.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7857..8744
                     /locus_tag="ACHBB7_001974"
     CDS             7857..8744
                     /locus_tag="ACHBB7_001974"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9209919.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8830..9060
                     /locus_tag="ACHBB7_001975"
     CDS             8830..9060
                     /locus_tag="ACHBB7_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM9209920.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9117..10082)
                     /locus_tag="ACHBB7_001976"
     CDS             complement(9117..10082)
                     /locus_tag="ACHBB7_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9209921.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            10388..10828
                     /locus_tag="ACHBB7_001977"
     CDS             10388..10828
                     /locus_tag="ACHBB7_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9209922.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            10838..11800
                     /locus_tag="ACHBB7_001978"
     CDS             10838..11800
                     /locus_tag="ACHBB7_001978"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM9209923.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
BASE COUNT         3815 a         2063 c         2411 g         3539 t
ORIGIN      
        1 atttgaaaag caattttcgt gaatttcagc ttatttccga aggagttgct tctgctttcg
       61 ccgtttatac gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt
      121 taattattat gttcaaaatt tgtttacacg gctaatgtag cgcctaattc atgttttgga
      181 atcgagattt caacaaggcc tgccaattcg attactttga tttcatcctc gtggtaaacg
      241 gcgtctttga ttttatctaa gaaataattt ttagcatctt cgatttcttt cggctggatt
      301 ttacgtttgt cattgatcag cagaatttca ttatgttcag gggcttcagt gtagacaata
      361 gtggtatttc cggctgtgta gaccttgact agatgggcat cagtgttttc aagctggttt
      421 aatacaagac gggaatggct gtttgttaca tttaccaatt tcatggcgct cacctctttt
      481 atgtattttt aattgcttgt ttctcttatt ataatttctt ttttataaaa tgaacagtat
      541 tttgactttt ctgaaaaaag aatgagacgg catttctcaa agaaataccg tctcacgcta
      601 taaaactgga tttattcagc cgtttcttcg gctgattctt tagccaaacg ttctggttta
      661 gaagtaacga tgatttttcc atctttatcc agttttgctt tcaactcatg gatagaaggg
      721 tgatcaagat aaaattcagc gataccatct tcgatttgtt cttggatcgt gcgtcgaaga
      781 ggacgagcac ccatagatgg atcatagcct aagtcaacta atttttcttt gacattcgta
      841 ggaacttcga tgtgaagttg ctgtgctgcc agcatttggt tgacatcgtc tagcattagt
      901 gtgacgattg tcatcaagtt ttcttttgat aatgcactga attcgatgat cccgtcaaaa
      961 cggttcagaa attctggtgt aaagtagttg tttaattggt tcaatacaga acgagtaaca
     1021 ccttcgcgtg ccgcaccaaa accaacgttt gcttctactt taccagttcc ggcattactt
     1081 gtcataatga tgatcgtatc tttaaagctg accgttcttc cttgtgcatc tgtcaatcga
     1141 ccatcatcta agatttgcaa gaacatatgc aaaacatctg gatgagcttt ttctacttca
     1201 tctaacaaga caagactgta aggatttctc cgtacttttt ctgttaattg accagcttct
     1261 tcgtatccga cataacctgg aggagaacca atcaatttag agacactatg tttttccata
     1321 tattcagaca tatcgaaacg aatcattgaa tcttgtgaac cgaatagttc gtaggcaagt
     1381 tgtttcgcaa gctctgtttt acctacacca gttggtccaa caaataagaa ggagccgatt
     1441 ggacgattct gtttgtttaa tccaacacgg tttcggcgaa tcgctttcgc tactcgatct
     1501 acagcgttat cttgtccgat gacatgagct ttcaagtcat ctgctaagtt tttcagttgt
     1561 gtctgttctt tttcttttaa ttcacccact ggaatacctg tccgttgttc aacgattttt
     1621 tccatatctt tttcggtaat cacaggtgtt tcctcttcag tgagctgacg ttctttcatt
     1681 ttttgaagtt tattgatctg atcgcgataa taagctgctt tttcgaaatc ttcttctcga
     1741 gaagctaaaa ctttttgttc ttctgcttct tgcagttttt tctcgattgt tttaggatca
     1801 acgatttgga tcgtcaagtt tttcttagaa cctgtttcat ctagcaaatc gatggcttta
     1861 tccggcaaga agcgatcttg gacgtaacga ttggataaag ttgcggctgc ttcgatggcc
     1921 tcatcagtat attttacatg atgatagtct tcataacgtt tttgcaaacc tttcaaaata
     1981 ctgatcgttt cttcgacagt tggttcatct acccgtacag gttgcatacg acgttctaaa
     2041 gcagcgtctt tttcgatgat gcggtactca tttaaggtag tagcaccaac catttgcaat
     2101 tctcctcgag ctaaagctgg ttttaaaata ttaccagcat ccatatttcc atcaccagca
     2161 gaaccagcac cgacgatttc gtgaacttca tctatgaata ggatcacatt ttctgcttga
     2221 cggatctctt caatcaattt ttgcatacgt tcttcaaatt gtcctcgtat accagtacct
     2281 tggactaaag atacgacatc taaacgaatg acttctttgt ccattaattt ttgcggtaca
     2341 tcaccatcta cgattttttg tgctaaacct tcaacaactg ctgttttacc aacacctggt
     2401 tctccaatca agacaggatt gttcttggtt cggcgattta aaatttcaat gactcgtttg
     2461 atttcgtcat cgcgtccgat cacaggatcg atatcacctt gtcgtgcttg ttctgtgata
     2521 ttgattccgt attctcccaa taaaccttct gtattgcctt gaggtggctg accaccattg
     2581 aagttgttgt ttccgccaaa ttgtgttggt ggtgtttgct cataatttcc ttgctgttgc
     2641 atttggcgag acatggaacg gaaaagatcg tccaaactgc caaatccaaa tggatcattt
     2701 tgggccatat tagtcagacc tccattttgt tgatttttga ttttttgata acagctctga
     2761 cagtaatcga gttgtgtccg ctgaccattg acagtggcat acaagtgaat cgttgcctca
     2821 ttcttgccgc agttttgaca aagcatgaat cattcacgtc ctttcacaaa aatgggtaca
     2881 tccatatttt acatctcgtt tttcatgagg taaaagaata acccctagtt ttttaagaga
     2941 tttatgaggt agaaaaggtg aaatctctat tgaccattcc tgacctttga ctcttattat
     3001 actaaaaata aaaagggaag caagtaattc gtttcatatg aaaacaaaga gggtcaaagg
     3061 aaagaatgat aaacgtcagg agaagagaaa gcgacaagaa aaaggaaata aacacgtttg
     3121 ttataaaatg tgaagaaaag gatgaaaaag aatccggaat aatttgaata cttttatctg
     3181 aaattgaatg aaaactgttg tatctaacga aaaaaaatgt taatctttac agatgaaagg
     3241 gtggcatttg ttaggattct ctgaattact aaaaaagaaa accctcacat aaaaacaaca
     3301 ctcacttaaa ggagaccgat taatatggaa aagaaagaat ttcacgtagt agcagaaact
     3361 gggatccacg ctcgtccagc tacactatta gtacaaacag caagcaaatt taactctgat
     3421 gtaaacttag agtataaagg taaatcagta aacttgaaat caatcatggg cgttatgtct
     3481 ttaggtgtag gtcaaggttc tgatgttact attactgcag aaggtgctga cgaagctgat
     3541 gcaatggcag caatcgttga aacaatgaag aaagaaggct tatctgaata atggttgaaa
     3601 tgctaaaagg gattgccgct agtgacggtg tagccgttgc aaaagcttac ctgctagttc
     3661 aaccggattt aacattcagt aaggcaacag tagaagatac tgcggctgaa gaagctcgtt
     3721 tggatgctgc attagctaaa tctactgaag aattgcagca aattcgtgaa aaagcagcgc
     3781 aaagcttagg tgaagcagaa gcgcaagtat ttgatgccca tttgatggtt ctttcagatc
     3841 cagaaatggt aggtcaaatc aagcaaaata ttaaagacaa cagcgtaaat gctgaatcag
     3901 cgctaaaaga agtaacggat atgtatatcg gtatgttcga agcaatggaa gataatgctt
     3961 atatgcaaga gcgtgctgca gatattcgtg acgttgctaa acgtatttta gcgcacttat
     4021 taggcgttac tttaccaaat ccatcaatga tcaacgaaga agtggtcgtt gttgcacatg
     4081 acttgactcc aagtgataca gcacaattag atcgcaattt tgtaaaagcg tttgtaacag
     4141 acattggtgg acgtacttca cattctgcta tcatggcacg ttctttagaa attccagcaa
     4201 tcgttgggac aaaagaaatt actgctaaag tcaaagaagg cgatattttg gctgtcaacg
     4261 ggatcgaagg cgatgtaatc atcgatccta cagatgaaca aaaagctgaa tttgaaaaag
     4321 caggtgcaga ttacgcagca caaaaagcag aatgggaaaa attaaaaaat gcagaaactg
     4381 tcacagctga cggcaagcat tttgaattag ctgcaaacat cggaacacca aaagatctag
     4441 ttggtgtcca taataatggt ggagaagcag ttggtctgta ccgtactgaa ttcctgtata
     4501 tggattctcc agacttccca acagaagatg accaatatga agcttacaaa gctgtattag
     4561 aaggtatgga aggaaaacct gtagttgttc gtacaatgga catcggtgga gataaagaac
     4621 taccatatct gcaattacct catgaaatga atccattctt aggatatcgt gcattgcgta
     4681 tcagtttatc tgaacaaggc gacgaaatgt tccgtacaca aatgcgtgca ttgcttcgtg
     4741 catctgttca tggtaattta cgtatcatgt tccctatggt agctacactc aaagaattcc
     4801 gcgcagcaaa agcgatcttt gaagaagaaa aacaaaaatt gataagtgaa ggcaaggaag
     4861 tatctgatac gatccaagtc ggcatcatga tcgagattcc agcagcagca gtcttagcag
     4921 acaagtttgc aaaagaagta gatttcttct ctgtaggtac gaacgatttg attcaataca
     4981 caatggcagc agaccgtatg aacgaacgcg tttcttactt gtaccaacca tataacccat
     5041 caattttacg tttgatcaaa aatgtaattg atgcagcaca cgctgaagga aaatgggcag
     5101 gtatgtgtgg agaaatggct ggagatcaaa cagctgttcc attgctagta ggtatgggtc
     5161 tagatgagtt ctcaatgagt gcgacatcta ttcttaaaac gcgtagttta atgaaacgct
     5221 tggacacagc gaaaatggct gaacttgctg atcgtgcatt gaaagaatgc gatatgatgg
     5281 aagaagtcgt tgaacttgtt catgaatatg taaaataaac aacgtatcaa aagctggtgt
     5341 ggcttgtaaa agagctacgc cagttttttt gttctttttt aagaaaacgt ttttgtcagt
     5401 aagaatagac aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat
     5461 ataagtgaag ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt
     5521 gcaaaatctg gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt
     5581 atctcttata cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga
     5641 atcaatagtc ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat
     5701 gcgcattcta cagaggaaga tttccgaaat tcctttatca gatcgaacca gctttctcct
     5761 atcgtaaaaa aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca
     5821 ccttattcaa aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc
     5881 aatgggatcg acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa
     5941 tatttcaaac tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt
     6001 aaaggaatca ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg
     6061 tttggtcatg ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat
     6121 catcctgaaa atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat
     6181 tcaggtgcag atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta
     6241 gaagctttag caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg
     6301 ttagaagata gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc
     6361 caacagatta ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa
     6421 gagcgaagta tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat
     6481 gaaaaagtct ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa
     6541 aaacatgaaa atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac
     6601 atcgatcaaa acattaaaac aagaattaga aagacatggg catgaagtct atatttttac
     6661 aacgaccgat cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc
     6721 atttatttct ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt
     6781 tgccaaagaa ctagaactag atcttattca tacccataca gaattcggag cagggatact
     6841 tgggaagatg gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta
     6901 tgaagactac cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata
     6961 cttgtctaga ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat
     7021 tgatacatta cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga
     7081 cattgaaaaa ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc
     7141 actaggtctt caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa
     7201 aaatatccaa gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag
     7261 attagttatc gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact
     7321 gcagttagaa gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta
     7381 ttataaagcg gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta
     7441 tacagaagcg atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg
     7501 attgttcgat catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc
     7561 acttattgat tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact
     7621 ttatgatatt tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt
     7681 tgtttatcat gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa
     7741 aataaaatta aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt
     7801 ttctagcgta tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga
     7861 aacttggttt tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag
     7921 caggacacga agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag
     7981 aaggagctat ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta
     8041 caatggtagg ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt
     8101 ctgcagatat ctcaggacat attttagttg atttgactac aagtacacca agcctggcag
     8161 aaaaaattgc aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg
     8221 gtggtgactt aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg
     8281 tttatgatca agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt
     8341 ctgctgggaa aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga
     8401 caggaatgac ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga
     8461 ttgagacgct ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta
     8521 ttttaaaaga agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga
     8581 ttgcattaga agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga
     8641 aactatatga agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca
     8701 aactttggtg gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta
     8761 gagaaaatca atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga
     8821 ggacctccga tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc
     8881 gagtcagcag ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt
     8941 acagtaactt atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag
     9001 caatacgaat ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa
     9061 attttaggat aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa
     9121 tttagttgct tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct
     9181 aattttttgc ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga
     9241 cgtttcaaag ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca
     9301 gaggtaatca ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc
     9361 tcatctgctg ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt
     9421 tcctggatcc gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct
     9481 ttttcgaaat tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc
     9541 tttttatttc ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt
     9601 tcctgtaggt caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc
     9661 atttgataag tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa
     9721 ggcccgtatg ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt
     9781 acataattat aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg
     9841 atcaattgac actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa
     9901 tctgttatat gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa
     9961 tagctgctta ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct
    10021 cggtctttca taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac
    10081 ataggatctc cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca
    10141 agttttaggg aattctatag ttctgttcac agaaaatact ttgataatca aaatatgtga
    10201 gatagatgat ttttctaata aaagaaatta taaatgctga tttatcagtt gttttaaatg
    10261 agtaaaatct taaaaaaagc tcatatagta aaaaaatgat cttttaaggt tgcattttga
    10321 aaaaagttaa gatatagttt gaacatcaaa agatttgata atcaaactaa ttagtggacg
    10381 gtggaacatg gaaccagatt taaaaacagt aaatgattat cttgttagtg tcttcaatga
    10441 tattttgaca atcgaagagt ctgagctgaa aaaatctcaa ttcaaagatc tgtcaatcac
    10501 agagatgcac acgatcgagg caatcgggat gtacaagaaa aaaacaactt cagaagtagc
    10561 caaagaacta tcaattactg ttgggacctt gaccactgct attaacaagt tagtaaaaaa
    10621 aggctatgtc gaacgtatcc gcagtgaaga tgaccgccgc gttgtaaagt tgggactaac
    10681 gaaaaaagga aagttactat ttcgggtcca tcagcatttt catcgtgaaa tggtcaagaa
    10741 catcttagac ggaatggcta cggaggaaca gcatgccttg ctggatgcac tgaaaaactt
    10801 gcacgacttc ttgcaagact ataagtgagg atatttaatg gaacaatttg gaatgattac
    10861 tgcaacagcc agcgtccttc ctgaaaagat tattactaat aatgatcttt ccaagatgat
    10921 ggatacctct gatgaatgga tttcgtcaag gacgggtatt cgtgaacgaa gatgtgtaac
    10981 aaatcaggag acttctgatt tatgcatttt agtggcggaa aaattgataa aacagaaaaa
    11041 tctaaaccct aaagatttgg attttatttt agtagcaaca atgtcgcccg attacacgac
    11101 accatctgta gcagcacaag tacaagggaa attgggagca actcatgcta tcgcttttga
    11161 tatcagtgct gcgtgctcag gatttgttta tgcgttaagc atggctgaaa aaatgatccg
    11221 ctgcggctca actaagggac tggtgattgg tggtgaaaca ctatctaagc taatcgattg
    11281 gtctgatcgt acgactgctg tgttgtttgg cgatggagca ggtgggattc tcttagaagc
    11341 gaatccacag aaaaaattgc tgaaagaaaa attagcagct gacggtacca gaagtagctc
    11401 attgaccgct ggctaccagc aaaataaaaa tccgttctat tcagaagatt cagaaggttc
    11461 gtattatttg caaatgactg gcagagatat ttttgatttt gccgtacgag acgttgcaaa
    11521 caatatccaa gaagtcatga aagacaaacc agttgattat ctgcttttgc atcaggcaaa
    11581 cctcagaatt attgagaaaa ttgcccgaaa agtaaagatg ccgcaagaaa agttcctgac
    11641 caatatggat aaatatggga atacatctgc tgcaagcatc cctatcttat tggatgaagc
    11701 agtttctagt ggaaagatca ccttaggaaa acagcagaaa gtaatattta caggctatgg
    11761 cggaggttta acatggggaa gtattctcat ggagttgtag actgttaaat tataaaaata
    11821 aaaagcaa
//
LOCUS       DAYYKN010000087        11523 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000087 DAYYKN010000000
VERSION     DAYYKN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.087"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..3257
                     /gene="fss3"
                     /locus_tag="ACHBB7_001979"
     CDS             30..3257
                     /gene="fss3"
                     /locus_tag="ACHBB7_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM9209924.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3369..3683
                     /locus_tag="ACHBB7_001980"
     CDS             3369..3683
                     /locus_tag="ACHBB7_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9209925.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3696..4070
                     /locus_tag="ACHBB7_001981"
     CDS             3696..4070
                     /locus_tag="ACHBB7_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9209926.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4080..4424
                     /locus_tag="ACHBB7_001982"
     CDS             4080..4424
                     /locus_tag="ACHBB7_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM9209927.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            4493..5509
                     /locus_tag="ACHBB7_001983"
     CDS             4493..5509
                     /locus_tag="ACHBB7_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9209928.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6103..7977
                     /gene="ltrA"
                     /locus_tag="ACHBB7_001984"
     CDS             6103..7977
                     /gene="ltrA"
                     /locus_tag="ACHBB7_001984"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM9209929.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8079..8459
                     /locus_tag="ACHBB7_001985"
                     /pseudo
     CDS             8079..8459
                     /locus_tag="ACHBB7_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            8504..9646
                     /gene="dcm"
                     /locus_tag="ACHBB7_001986"
     CDS             8504..9646
                     /gene="dcm"
                     /locus_tag="ACHBB7_001986"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFM9209930.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9636..9925
                     /locus_tag="ACHBB7_001987"
                     /pseudo
     CDS             9636..9925
                     /locus_tag="ACHBB7_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10181..11365
                     /gene="mobT"
                     /locus_tag="ACHBB7_001988"
     CDS             10181..11365
                     /gene="mobT"
                     /locus_tag="ACHBB7_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM9209931.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11362..11475
                     /locus_tag="ACHBB7_001989"
     CDS             11362..11475
                     /locus_tag="ACHBB7_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM9209932.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         3862 a         1975 c         2570 g         3116 t
ORIGIN      
        1 ttgtgcctaa tttgaaagga gaacatttca tgtataaaaa acaaaaaaag aaattatgtg
       61 tattaactat tttgagtttg gctgttcaat caattgtagg agcaattgct cctacgattg
      121 cttttgctga tgaaattacc catccacaaa cggtaaccgt agaactagat ttagcacatc
      181 aatatgctgt tgaaggaact tttagtgatg gtcgtccaat gtcagaagtc actgttccgc
      241 attatgcagt ttacaatggt gtgaagcaag atattttctg tgttgaacca ggagtcccga
      301 tttacaatga gttcactcct ggatatgaga agaacccgtt accagatatg tccgataaag
      361 ccaaattagt ttccgtttta tggaaaaaag taggtacaga tgcagatact catattgtgg
      421 cacagaagat gatctggcaa gaagttaatg ggtataccct tcactcaata aaacgatcag
      481 atggtagtgc cgtaaatatc gcagccattg aggcaaaaat caatcaagcc attgccgatt
      541 atcagaagaa accaagcttc cataatagta cggctaaaac agtattaggg caatcgacta
      601 ctgtgacgga tacgaataat ctgaatttgt cagagttcga tgaggttgtg gaaaataccg
      661 caaacattga ttatcgtgtg aatggcaatc agttgataat cagcccaaat gccaattcaa
      721 aagaaagtgg tgtgttgacg cttaagaaat ctgctggtac cggaacacct gtcgcctata
      781 aaaaggctgg ccaacagact ttaatggccg gggcgatcga taagccgaac acttatacgg
      841 tcaaaattga tgtggaaatg gagggttctt tgaagattaa aaaagttgat aaagaatcgg
      901 gtgatattat accaggaacg gttttccatt tagactttgg aaaaacttta cccgcaaaag
      961 acgtgaccac cgataaagaa ggcattgcga ccttagatga gattcctcat ggtacaaagg
     1021 taaccattac tgaaaaatcg gtgccggcgc cttatacgat tgataccaca cccatgactg
     1081 ccacaattaa agcaggtgaa accatttcag tgacttcaaa aaatacgcga gaaaaaggtc
     1141 aaattattct ggataaaacc ggaattgaaa cagggagtga tctttggaac gataattact
     1201 ctttagcggg caatacgttt gccattcgca aggatagccc aactggtgaa atcgtccaag
     1261 aaatgaccac ggatgaaaaa ggtcatgcag aaacaccaaa agagattgcc aatgcgttgg
     1321 aattgggaac ctactatgtg actgaaacca aggctagtca tggctttgtg aatactttca
     1381 aaccagtaaa agtcgaattg aattatgtca atcaaacagt ggctcttgtt accagtaacg
     1441 taaaagggca aaaccaagaa attactggtg aaaccacttt gacaaaagaa gacaaagata
     1501 ccggtcatga aactcaaggg aaagctgagt ttaaaagagc tgaatacacc ttgtttactg
     1561 ttaaggatgg aaaagcggtc aaatggaccg agaactttaa accagaattg ttgaagggaa
     1621 cgaaagcttc tgatgaaacc gtaactttga ctttagatga aaagaatcaa gttgccgtta
     1681 aacacttagc gattaatgag tattactggc aagaaaccaa agcgcctgaa ggatatacct
     1741 tggatgaaac aaagtatcct gtatccatca aaaaggtgga tgatagcgaa aaaaatgctg
     1801 tgattactcg agatgttgcg gcaaaagaac aagtgattcg ctttggcttt gatttcttta
     1861 aatttgctgg atcaactact ggcactgccg aaactggctt caatgatcta acctttaaag
     1921 tgtcgccatt ggaaggaacc aatgaaatca ccggtgccga agatgaagcg accacagctt
     1981 ataacgagca attagggttt gatggttatg gcaagtttga aaatcttcct tatggggatt
     2041 atttacttga agaagtagaa gccccagaag gttttcaaaa gattacgcca ttagaaatcc
     2101 gttctacttt taaagaaaat aaagaggatt ataccaagag tgattatatc tttactatta
     2161 ccgaaaaagg gcaaaagcaa ccgattaaaa cggtaacggt cccttatggg aatctaacca
     2221 atgagaattt ctcagtcagt ttgaaccgtt tgatgcttta tgatttgcct gaggaagaag
     2281 atagcttaac ttctcttgcg acttggaaag aaggagataa agaattgact actcttgatt
     2341 ctaccgagct agtcgataaa ttgagctata atctacatga aatcaaagaa gactggtata
     2401 tcgtagctca agccatcgat gtagatgcta caaaagctgc ccaagaaaaa gataaaaaag
     2461 ccaaaccggt ggcgattgcg gaaacaaccg caactttagc gaataaagag aaaacaggaa
     2521 cttgggagat tctgcataaa ttaactgtcg aacaagtttt agacaagacc attgtcttat
     2581 tcaactatgt gtatgaaagc aaagaagcct atgaagctgg tgatgagccg gtagcgaagg
     2641 atgctagctt gaacaatcaa gcgcaaactg ttaattgtat ggtggaacgt catgtttcca
     2701 tccaaaccaa agcgcactta gaagaaggtt ctcagacctt tactcatggt gacgtggtag
     2761 atatgtttga tgatgtatcc attacccatg atgtactaga tggctcaaaa gaagcttttg
     2821 aaacaattct gtacgcttta tttccagatg gtacaagtaa agaaatttgg aaatctggca
     2881 agattgatta cgaagtgaat gacaaagaat ttaccaaaac cgtacttgcg gaaaaagtgg
     2941 atacaagtaa atatccagaa ggtacaagtt ttaccttcaa agaaattaac tatgacaaag
     3001 atgggaatat caatggaaaa cacaatgaag atttgaaaga aaaatctcaa actttaacgc
     3061 ccaaaaaagt tccgacaaca ccaagtacac cagaacaatc tgaaatgcca actgttccaa
     3121 gtggctctca agaatctagt cctacagtga aggcattccc tcaaaccggg gagaaaactt
     3181 ccaatgtgct actgttcatt ggctttagct tgatctttgc gacggctggc tattattttt
     3241 ggaatcgtcg gaactaaggt gataagatga aacatgtaaa tagtagaaaa caatacgcgc
     3301 gaccgcctcc taaaaaagtg gtcgcgctat ttttgtattc aaaactaaca atgaaaagag
     3361 gaaaaaatat ggaattgaaa tttgttgtac cagatatggt cgagacgttc ggaaaactaa
     3421 catacggtgg tgaaggtgaa gttctatcgg aagggtatgg tcgaaatact acaattgttg
     3481 gtcgaagtta ccatctgttt tcaaataaac aacgggccga tgatattgaa gtggtggtag
     3541 ctgcggaagc cggcgaaaag gattttgacc aagatcaacc actaaaagct gtaaatcccc
     3601 acttaattgc caaaggctat gaaattgaga atcgtgggtt taccgattac gtgttgtatg
     3661 ttgatgattt agtgaaagca taggaggaaa agaagatgag attaacagaa ggtattgtcg
     3721 tagattcagg attaaccttt ggcaagttgc gtttttccgc attacgtcga gaagtacgca
     3781 agcaaaatga ggacggaacg gttagtaacg aggtaaaaga acggacctat aatttgaaat
     3841 cctccgcgca aggtcgaatg atccaagtca gtattccagc aaatgtaccc ttacgcgagt
     3901 ttgcttatga tgcagaggta gagctggtca atccaatcgt ggatacggtc gccaattatg
     3961 tttttcggga aggaaccacc gttaattggt ttatcaaggc agatgattta gtcttgaaac
     4021 gacaactgaa tcaaggaaaa cctacgaacc aaaatgaagg aaagaaatag gtgaagaata
     4081 tggaaattaa tgttgaacat attttagatt gtctcgatca atttggcaaa ggtgagctaa
     4141 cggaggaaca actcactaac gcattaacct atgatgaaaa aatgtttctg attatgcatc
     4201 aagggttact ggaagtaggt aacgatgcca aagaagactt tgatgtactg gattggttag
     4261 gggaagaaga gtctttcttt atggttgtcg aggtaaatga aagtttatgt cggcaggcag
     4321 aatctgtttt ggaagaaatc gatgtggaga tgccggatgc catcgaaaga tttttaaaac
     4381 aattagtgga aacgaaacaa cttcccgtag tagtaaatga ctagcataac tttttgaaat
     4441 tgcccaatga aatcctattg ggctttttcg actggaggtg gaaaccatca aaatgtacaa
     4501 aggacatcgc atacgcgcag gagatcaaca cttgatttat cactttgttc ttggctggct
     4561 aatctttttg ttcattggtt ggatgagcgt cttttattta caagaactta gacaatttga
     4621 tatttctaag gtatcatttt ccactagtga aattggctgg tctatgaagg atgtaatctg
     4681 cttgctagga agtctcgctc tttcaggaag catgatgtta ctttatatac acttttttca
     4741 ggatcattgg cgaagtcttt ggcatcgaca aaagttagct cggatgattc tagaaaacca
     4801 ttggtatgaa gtaaagcaga ctcaaggtga aggttttttc aaagatttga atagtagttg
     4861 gaccaaagag agtatcagtt acttccccaa aatttattat cgaatgaaag atggcttact
     4921 ttctattcgc gttcaaattt cactgggaaa atatcaggat cagctattga agttggaaaa
     4981 taagttagaa agcggactgt attgtgagtt agtagaaaaa gaactcaaag actcctatgt
     5041 ggaatacacc ttgttatacg atatgattgc caatcgaatt gggatagaag aaatagtggc
     5101 agaaaatggg gcgttgcgat tgatgaaaaa tcaagtatgg gcgtatgatt ccttgccgca
     5161 tatgttaatt gctggcggca caggtggtgg gaagacctat ttcttactca ccattattca
     5221 agcactattg aagtcagatg cagaattgtt tatcctcgat cccaaaaatg cggacctcgc
     5281 cgatttaggt acgataatgc ctcacgtata ctcccaaaaa gaagaaattt ctgaatgtgt
     5341 ggaagatttt tacgaacgca tgatagctcg tagtaggtca atgaaagaaa tgcccaacta
     5401 caaaacagga gagaattatg cgtatctcgg acttccgccc aactttttaa tctttgatga
     5461 atacgtggct tatatgggtg cgaatcgttt tcctacaagc atcgaataga tgtgaaatgg
     5521 aagcctattg agattatcaa tagtgacaac taatgttttg agagatggaa tgaaagcctt
     5581 caagttgagc tgaaacatcc ccgctaatac tcctacatgc tctaatcaat gaagtagatt
     5641 ggttttggta tgaagctagg tgaagtcgtc tgaaatctgc ctaagtgctt gagcataagg
     5701 ctgactatag ggcgggcggc tgaaaaagat aactatggta agaatgtctg aaaatgactg
     5761 acgaatctgc gaatgtacgg gtctaaaagg ctgatcttat tgaaagatag gaacatacgg
     5821 acgtatggtg agataccgac aagtaaggtt ccatgatatg aaaatcggga tagctaacga
     5881 agaagctata atttcacagg ctcatagtag atacctaagg ttatacatga tagctaaaaa
     5941 cattggaacg atgtaagcag aaaactatca attaaatgac tacttctaga taggtgcata
     6001 taagtaataa cgaaattgct ttgtttctgt gaaagtaagg gcacagtacc gatgaagcgt
     6061 ctaacaaatg tggagggata gccctaagtc tatttaataa caatgaatgt tgaaacagat
     6121 gaagttcaag gatctggtaa gaacgtggga aaatccgtta tggaggtgcc acgcttgaac
     6181 acaaaaatac gttactggga atattatgat ctacaagaaa cattcgatca gctatttact
     6241 caaagtagaa acgggaaaaa gttttatcaa ttatatgagt taatcatttc tgaaaataat
     6301 attcttttag cataccgtac tatcaaggca aacaaggggt cttcgacacc aggaacggat
     6361 tccttcacaa ttgataacta taaggaaatg aaccaagcag agtttatcca tcttattctc
     6421 agtcatttag aaaattacaa accaaaatcc ataaagcgag taatgatacc taaacccaat
     6481 ggtgagaaac gcccattagg aattccatgc atgattgatc gaatgatcca acagatgttc
     6541 aagcagatac tggagccaat atgtgaagct aagttttatg aacatagcta cggtttcaga
     6601 ccattgagaa gtgcaaagca tgctctaggt cgcattatgt atctcataaa catcagtaaa
     6661 atgcactatg cagtagacat tgatatcaaa ggtttctttg ataatgtaaa ccaccgttta
     6721 ctgataaaac aattatggaa tataggaatt tgtgacaaac aagtcttagc tattctttca
     6781 aaatctctga aatcacccat tcaaggagaa ggtatcccga gcaaaggaac gatccaagga
     6841 ggtatcattt ctccactatt atctaatgtt gttctaaacg acttagacca ttgggtctca
     6901 aagcagtggc atacctttga aacaaagtat ccctatacta aaggttacaa taaatttcgt
     6961 gctctaagag atacaaatct aaagcaaggc tatattgtca ggtacgcaga tgactttaaa
     7021 atcatgacta atgattatcc gacagcatta aaatggttcc acgctgtcaa gctttaccta
     7081 aaagacagac ttaaattaga tatttccaat gagaagtcta aaatcgtcaa tttgcgtaaa
     7141 tgtaagtcgg aatttttagg attcgctatt tgcgtgaaac aaaaagggaa aaaatgggtt
     7201 tgtaattcac gtatttctaa caaaaaaaag gatcagatta aagaagagat taaacaaaga
     7261 atcaaagata ttcaaaagag tcctacagca caaaatgctc tcctgtttaa tgctctcgtg
     7321 ctcggtgttc ataattattt caggtatgcc actcatatta atttagattt gaataggatg
     7381 aactacgttc tttcattcac tacttacaac cgattatcaa attgtagtac aagatgttac
     7441 cccaacaaag cttccccaac ttacaaaaag ttttactcaa caacgatgaa aacgtataaa
     7501 gtagctggag tctatttgta ccctctttgc gacgtatcta caaaaacaat ttacggattt
     7561 aatacggagg atacaccgtt tactccgttt ggtcgtcaaa gattggagca caaaagccta
     7621 cagtctcttg tgttccaaga attaagaaaa ttgatggaat cgaaaatacc gaatagaacg
     7681 gttgagtatt tagatagtag aatatctcgt tactcaatga aatcaggcaa gtgtgagatt
     7741 acaaaacaat ttttgccagc aaaagccgtt cactgtcatc actatttgcc aaaaagtctt
     7801 ggtggagacg acaaattcga taacttgaga attattcata aggatatcca tcttctgatc
     7861 catacaacaa ataaaatgat aatcgatcac tacgtgcatg aactgaaact ggtgccagag
     7921 caaatagcaa aaatcaacct ttatcgaaag atgtgtaatc tgcaaaacat tcaatagaaa
     7981 ataaatagat ggaacgccgt atgaggtgaa agtctcacgt acggtgtgga gcgggggaaa
     8041 agatggagat tatatcaaat tcttacctat cgctatagat gttaacgaca aaagaaagtg
     8101 cggtgatttt gaataagctt aaacaaatcg tgatgttagg ccgtcaatct ggtttctttc
     8161 tgattctagc ttgtcaaagg ccggatgcga aatatctagg agatgggatt cgcgatcagt
     8221 ttaacttccg tgtggctttg ggtcgtatga gtgagcttgg gtattccatg atgtttggag
     8281 aagtcgataa aaattttttt atgaaacgca tcaaaggacg aggttatgta gatacagggg
     8341 gcagtgtgat tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttaaggg
     8401 aaattagcaa cattgttgga ttaaccgttc atacggaaag agaaaataat agtgtgtagc
     8461 ctaggaatat caataatcct gggctttttt attgaggtga tttatgacat ttttagattt
     8521 attcgccggg attggtggct ttcgcttagg tatggaacaa gccggtcacc agtgtatcgg
     8581 tttttgtgaa attgatgaat ttgctcggag gagttataaa gcaatccatg atacaaggaa
     8641 ggaggtggaa atgcatgaca tcacaagtgt atcagacgaa tttgttcgat ccctcggacc
     8701 agtggacatc ctttgcggcg gctttccgtg ccaagctttt tcaattgcgg gaaagcggca
     8761 aggattttcc gatactcgag gtactttatt ctttgaaatc gctcggttcg ccactcttct
     8821 ccaacctaag tttctattcc ttgagaacgt ccggggattg ctcaatcacg aaggaggggc
     8881 tacgttcgag acgatcctcc gaacgttgga tggattgggg tatgatgtgg aatggcaagt
     8941 gcttaactca aaggcctacg ttccccaaag ccgtgagcgg atcttcctta tcggacattc
     9001 tcgagatgca tgtaccgaac aagtatttcc tatcattgga tcgtcttcaa catctgatca
     9061 aaacatcaga aacttggtaa atatcaatcc ttccaatcga ggaatgggtg gccaagtcta
     9121 tggttcagat agcgtagcac caaccttgac cggtgatgaa ggaattaaaa ttgccttacc
     9181 ggtaaaggat gggatttctg tagcgggtat gttgtcggga aattttgaac aaggcaatag
     9241 gatttatgag gtaaccggaa cagcccccac tttgtcaacg aaacaagggg gaacgaagat
     9301 tatgattcgg aaaaatgaaa cagaatatca agaggtgaag ccaggggaca gtgtgaacct
     9361 ggcttttcct aattccacaa gtcgccgagg tcgtctggga aaacaaagtg tgcatacctt
     9421 actaacagga gaccaacaag cggtagtcac ggatcagtac cagattcgaa agttaacacc
     9481 tagggagtgc tggcggcttc agggctttcc agattgggct tttgatcgag cttctcaagt
     9541 caattcagat agtcagttgt ataaacaggc cggaaactca gtaacagttc cggtcatttt
     9601 tgatattgct agaagattaa agggggtaaa agcatatgat ttatgacgag ttgattggag
     9661 aaatctactg gatgattggc aagattcagg cagatccgaa attggaagaa gaactgtacc
     9721 ggctcaattt tgatattcgc aaaaacgggg tgaaagtcca ggtgattcgt atgtagtgga
     9781 tgaggaaacg gatgctcgaa tggagctcaa tcaagtcatc ggtgaattgg aacgaattgc
     9841 ggaccttgcc aaagaacctg atatccgcca atacttgttt gagataaaag cagagttgga
     9901 aattgagggt atcaccgcgg agtaataggg aaataaccca ctgataggca ctgaccgcgg
     9961 gggatttgcg aagcagaaat cctccgtggt gctgtgcctg ttcatggacg gcggcagccg
    10021 gccatgatgt ctcacccccc gtatctaaca ggggggtaca aaaacacaaa aaacaagcaa
    10081 gtaaccataa aaaatgcttg ttgtatcaag gatattaagt aatttgtata ggtgttaact
    10141 catttggaaa agttaacacc ttgaatttgg aggtgaaaac ttggcgcaac gaaatttaga
    10201 ttatcggtta ttaaaagaac gacgcaacga atatggcgtt tcacaaaata aattagctac
    10261 tgcttgcgga cttagccgcc catacctaaa tcagatagaa aacggtggcg taatcgcctc
    10321 tacaaagacc atgagaaaaa tttttgatca actggaaagt ttcaatcccg atttaccttt
    10381 aacgttacta tttgactatg taaggattcg ctttcccaca acggatgtgc ggaaaattat
    10441 tcaagaaatt ctccatttga aattcgagta tatgcttcat gaagattacg ccttttactc
    10501 ctatcaagaa caatatgtca tgggcgatat tgtagtgatg ttatctcatg aagaagataa
    10561 aggtgtcctt ttggaattaa aaggtcgtgg ttgccggcag tttgaaactt ttttactcgc
    10621 ccaaaagcgt agctggtacg acttttttga agattgtcta aaagccggtg gcgtgatgaa
    10681 acgcttggat ttagcaatca atgatcgagt ggggctactg gatattcctg atttaacgaa
    10741 aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc ttcaaaagtt accgttccgg
    10801 ggaactgttg aaagctgatg aaaaggatgg tatgggcaat accttatata ttggcagcct
    10861 caaaagcgag gtttattttt gtttgtacga aaaagattat gaacaatata tcaagttagg
    10921 gattccattg gaccagaccg aaacgaaaaa tcgttttgag attcggttga aaaatgaccg
    10981 tgcttattat gcgattcaag atttgttaaa aggtcgtagc attgaaagta ccacgttttc
    11041 cattatcaat cggtatttgc gatttgcgga taaagtagat ggaaaacggc gaaccaattg
    11101 gcccttgaat gaacaatggg gtcgttttat tggacggaat cggaaagaga ttcaattaac
    11161 ttctgaaccg aaaccgtata ccattgaacg aacattaaat tggctaggta gacaagtcgc
    11221 ccctacgtgg aaaatggcta aggagttgga tcgattgaat caaacaacgt atatccagga
    11281 tatggtacag aatgcgcgac tctcggaccg gcataaaaag attttggagc aacaaagtat
    11341 ggcaatcgaa aacttgattg tatgaaaaga ggaattttga tggaaattgt aaaagatatg
    11401 ttattggtac ttggtggcag tatgcttggt gttaccgtga tgtgcttgat gaatgcaagt
    11461 gcagcagcgg attgagcaat ggaaaagaaa ggaaagtgac aaaatgaatt tcggacaaaa
    11521 ttt
//
LOCUS       DAYYKN010000088        11418 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000088 DAYYKN010000000
VERSION     DAYYKN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.088"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..273)
                     /locus_tag="ACHBB7_001990"
     CDS             complement(49..273)
                     /locus_tag="ACHBB7_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM9209933.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(471..2051)
                     /locus_tag="ACHBB7_001991"
     CDS             complement(471..2051)
                     /locus_tag="ACHBB7_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM9209934.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2452..3828)
                     /locus_tag="ACHBB7_001992"
     CDS             complement(2452..3828)
                     /locus_tag="ACHBB7_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM9209935.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3956..5044)
                     /locus_tag="ACHBB7_001993"
     CDS             complement(3956..5044)
                     /locus_tag="ACHBB7_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9209936.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5216..5650
                     /locus_tag="ACHBB7_001994"
     CDS             5216..5650
                     /locus_tag="ACHBB7_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9209937.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5728..6261)
                     /locus_tag="ACHBB7_001995"
     CDS             complement(5728..6261)
                     /locus_tag="ACHBB7_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM9209938.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6355..7533)
                     /gene="mutY"
                     /locus_tag="ACHBB7_001996"
     CDS             complement(6355..7533)
                     /gene="mutY"
                     /locus_tag="ACHBB7_001996"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM9209939.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7526..8323)
                     /gene="recX"
                     /locus_tag="ACHBB7_001997"
     CDS             complement(7526..8323)
                     /gene="recX"
                     /locus_tag="ACHBB7_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM9209940.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8430..9812
                     /gene="rlmD"
                     /locus_tag="ACHBB7_001998"
     CDS             8430..9812
                     /gene="rlmD"
                     /locus_tag="ACHBB7_001998"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9209941.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9986..10564)
                     /locus_tag="ACHBB7_001999"
     CDS             complement(9986..10564)
                     /locus_tag="ACHBB7_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM9209942.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10886..11356
                     /locus_tag="ACHBB7_002000"
     CDS             10886..11356
                     /locus_tag="ACHBB7_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM9209943.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         3359 a         2291 c         1947 g         3821 t
ORIGIN      
        1 agcttatttc ttggagttaa acgcttctgt ctcagcctct ttttctttct agctttttga
       61 tttttctctt ttcttttgta cttttctctt ttcattttca gttctacgcg tgtctttatt
      121 tttaactttt tcctctttat ggatgattga tcgtactttt tgtgcaattt gctcgttttt
      181 gtcccagtct tttacatcta ataatcgaca taatcgtaac atctcttctt ttttctgttt
      241 ttttctttca cgtctttgcc attctgtttc catgtgtcac actctccttt ctttaatcat
      301 atgtacgcaa aataattttt atattttttt gattagacat ttctatgatt ttaatatagt
      361 aaaaagagcc gtcacggaaa atccgtcacg gctctaaaca aaatatgaga ggctgttttt
      421 gtttggctat tcttattact tgatgcaggg cagcttgtgt caagacttct ttacatcaaa
      481 ctttttaatt ttacattggg atatttatcc gcaaaccagc gttctgcaaa ttggttctcg
      541 aataagaaca acggttggtc gaaacggtca cgagccaaga tattgcgact tgaactcatc
      601 ttctcatcca aatcttctgg atcgatccag cgagcgattt tgtgtcccat tggagacatg
      661 attacttcgg cgttatactc atttaacata cgatattgga atacttcgaa ttgtagctga
      721 ccaacagctc caatgatata ttcttccgtt agatatgttt tataaagctg aatcgctcct
      781 tcttgaacta attgatagat tcctttgtgg aaggattttt gtttcatgac attttttgca
      841 gtcactttca taaacagctc tggagtaaat gaagggagtt cttcataagc aacttttagt
      901 tttccttcga acaatgtatc cccaatctga taattgcctg tatcatatac accgatgata
      961 tcgcctgcta ctgctttttc gacgttttct ctcgaatctg ccataaattg tgtgacattg
     1021 cttaatttca gttttttatt tgtacgttcc aaccagacat ccattcctcg ttcgaatgtt
     1081 ccagaacaga ttcggacaaa agcgattcgg tcacgatggg ctggattcat atttgcttgg
     1141 atcttgaata cgaaacctga aaattcttcc tcataaggac ttaccatctg atcatcttgt
     1201 gttttgtgtc cataaggagc tggtgccaat tcaacgaaag tctctaaaaa agtctgtaca
     1261 ccgaagttag tcaatgcaga accaaaaaat actggtgtct gatcgccacg agcaattttt
     1321 tcttgattaa atgcatctcc agcctctttg attagttcta cttcttctaa gacttgttca
     1381 tataaggaat tttcagttag aggatgattt tcgggaagca gtccatcttc atccaattct
     1441 actaatcgtt catcctgata attttccgga cgataaaact ctacacgatt atggtagata
     1501 tcataaagac cttctagccc tttacccata ccgattggcc aattcatcgg ataagattcg
     1561 atatctaata gctcttctaa ttcttctaac aattccaatg gttcacgacc atcacgatct
     1621 agtttgttga taaaagtaaa tatcgggatg ccacgttttt ttacaacttg gaataatttc
     1681 tttgtctgtg cttcgatccc ttttgcacta tcgattacca tgaccgcact atccacggcc
     1741 atcaaagtac gataagtatc ttcagaaaaa tcttcatgtc ctggtgtatc caagatattt
     1801 actcgtttcc cttgataatc aaactgcata acagaactag taacagaaat cccacgttgt
     1861 ttctcgattt ccatccagtc tgatttggca aaattacctg tttttttgcc ttttacggtc
     1921 ccggcttgac ggatagcccc accaaacaat agtaattgtt ctgtaatcgt tgttttccca
     1981 gcatccggat gggaaatgat cgcaaaagtt ctgcgattgt ccacttgttg ctttaatttt
     2041 ggattattca taacctaact ccatttcttc cattcaatta tttatatttt ctctaggcac
     2101 agattgtccg atctcttaaa gaaagacaat ttcatatagg gctgtgacaa aagccgtgta
     2161 gcagtctctt tttccgagta aacggcgttc tagagacagc ctatcagcaa taagcccacc
     2221 caagccaaaa atacgaaaag ctgtttttgc ttggctgctc ttattactta atgcaggata
     2281 gctttttcac aacctttgtc aactactaca actgaatcag tatagcatgg attcagcgaa
     2341 tgaaaaagcc accaaaccat aagagtcaaa tatttttttg tcagctgact aaaagaaaag
     2401 acgtgttgct tgtttattca agcaacacgt ctttcataac caagtttcca attatcttct
     2461 tggctcgtca tcttcgaatt cttttctgaa gtatcttcgt tcttcttcag gttccgcttc
     2521 tgtttcttcc ggatcatgaa aaatgacacg gattttaagg attcgagaac cttccatttc
     2581 ttcagaaacc agcgtaatat tatcgacatc aaacgataac ttttcgcctt catccggaat
     2641 catgcctaat gctgtaatca aataaccagc catcgtatct acatcactca tatgcagatc
     2701 actgtcaaaa gcttcattga actcatcaat cagcatacgt ccttggatga tatattcgtg
     2761 ttcatcgatt ttttcataaa gattctcaac ttcatctgat tcatcatcga tttctccgac
     2821 gatttcttct aaaaggtctt ctagtgttgc taacccaacg acgccgccat attcatctaa
     2881 taaaatagcc atctgattct gcgtcttttt catttcatat aacagatcat caataaagac
     2941 cgtttcagga acgaataaag gctcctgcat gattttcttg atatctaaat tatcaaagcc
     3001 gaatttatgg gcagctttta gtaaattctt tgtatgaagg attccgacta ctttatcttt
     3061 gtcttcgtta taaacaggga ttcttgaata gttttcggat aaaacttcat tcacattttc
     3121 ttccacagtg tcgttgatat caatcataaa tgcatccgtt cgtgggacca tgacttctcg
     3181 ggcaactttc gtatcaagag aaaaaacccc ttggagcatt tctaattctt cattattcaa
     3241 tacgccttcg ttttcaagca tataacgcat ttcatctctg gtcatttttg aatcttcatc
     3301 atcaaaagtc atcggcgtga tacgcgccaa taaacttgtg gaagcagaaa gcagccagac
     3361 gaagggctta gctactacac caatcaagcg tatcagccct gaagtgaact ttgcgacttc
     3421 ttctgattta tttaaagcaa tacgtttagg gtacaattct ccaaaaacaa tcgatacata
     3481 ggtcaaaata gccagcacaa tgatatttgc aatatttttt gccgctgtgc cgccacctaa
     3541 taccggagct aaccgttccg ataaagtatt agctagtgaa gcaccagaca aaatgttgac
     3601 caaagtgatt cctacttgga ttgtcgatag gaaattattt ggattctgca atatttttaa
     3661 aagcttttga gatttcacat ctccttcatc agctttttgc tcgattcgat tcttattgat
     3721 cgaaacaaca gcaatttcgg aagctgctaa aaaagcattg atcaatgtca aaacgatcaa
     3781 taataaaatc tgcgctaaca gcgactgact ctcagggtca gcattcataa tcgcattctc
     3841 tccttataaa attaattttt agtttacgat agaaaaaaac gtggcactat agccacgtta
     3901 aaatggtatc acaaagcaat tttctttgca ataaaaacaa gtgtactaaa actacctacg
     3961 aggtcgtagc aattttcttc ttgttttcat tttgcttgtc ttctttgacg attttgatta
     4021 tataggaaat gatcgtcttg cagattgcgt aaaaaggaac acctaagaag ataccaagca
     4081 atccggcaat atttccagct actagaagaa cgataatgat cgttaatgga tggattttca
     4141 gtgatttccc aattacatta ggataaatga tattcccatc caactgttgg acgattagca
     4201 caaccacaca acaaagtaat gcttttaccg gatcattgaa gactgttact agaaatgcag
     4261 gtgctagtcc tagataaggt cctaaatatg gaattaggtt tgtcaatccc gcaatgaccc
     4321 caaataagaa agcataatcc acaccaatta ccatatatcc taaaaatgtg aaggtaccaa
     4381 cgaataaaca ttcgattgct tgtccgctaa tgtaattagc caatgtttta ttcagttgcc
     4441 ccaataactc cacaatttgg tttcttcttt tttctgggaa aaactgttgg atgtttggca
     4501 ctagtttttc tccgtctttc aacatataga acaagatgaa tggtgctgta atgattacta
     4561 cggttgccga agcgatcgtt ccaacgatcg aacctaaact attggatact ccactaagga
     4621 attgctgaat gattgtcccg taagaaatgt ctaattgctc aaaatattta ttgatatcta
     4681 cttccttgaa caaaggcaat tcggctacct cttttaacca gctttctacc tgtttgatga
     4741 aatcaggcat attagatgcc aatgaggaaa tttgctgtac tagacttggg atgacactaa
     4801 gtaaaatcca aataattgct gcaactaaaa ggagcagcac aattgcgaca gcccatattc
     4861 ttttgatttt tgctttcatc aacagcttga caataggatt caataaatag taaagaaagc
     4921 ctgcaatcag tacaggtgca aataacgtgg aaaaaaatgt cccgatcggt tcgaaaataa
     4981 agtttatttc tgatgaaatc atgatcaatg cagctatgat caataattca agcgaccaaa
     5041 acattagttt agactgtcta agtttttcaa acaatagatt tcctcctttc tttcttacta
     5101 gtatatatta tattcgttaa aaattctttt attctttttt tatttttgaa aacttgccat
     5161 actttttatt tgttttattt tccagtatac tatacgaaag gaaggtggct agaccatgaa
     5221 agttgtacaa acaaaagata caatgagcaa tatttactta gatgctgtac ggatcaggca
     5281 tcaagttttt gtagtcgaac agggtgtgcc tttgtctcga gaaatagaca aagatgaagc
     5341 ccattgtatc cacttcgtcc tgtactcaga taaaaaagaa ccgcaaggaa ctgtacgtct
     5401 tttgccttta gaaaacggaa aaatgaaatt acagcggatg gcgattcttt ctgaatacag
     5461 acaccaaggg ttaggaaaaa tattgattga agaagcagaa aactttgcta agaatcaagg
     5521 ctataatacg attttattag gggcacagtc cacagctgaa actttttacg aaaaactagg
     5581 atatacagct tacggcgatc cttttgaaga tgctggcatg cctcatattt atatgaaaaa
     5641 gactttataa aaatttattt cgaaaaaagc aaaaaacagc aagcttttga gaaaaatctc
     5701 tcactggctt actgttcttt tttgtgttta cttacgcgac aattgcttgt aacggttata
     5761 ccaaatatca atatagcctt ccgagaacgg gccatctcca ttattgatcc agtcaaccag
     5821 aatcttcaca ttttctttta ggatgaagtc aatatcgttc gggtaattca tcattttgct
     5881 gtgcatctca tattcatcga catccaatag tcgtttctcg ccgtcaggaa ataccttgat
     5941 atccaagtca tagtcgatat atttcaaggc ctcatcgtcc agcaaaaaag gtgaagctag
     6001 attacaataa taggaaaccc ccttttctct gatcattgct attacattga accagtattt
     6061 tttatgaaaa taaacaatcg ctggttcgcg cgttacccat cggcgtccat cagattcagt
     6121 gactaatgtg tgatcattca cgccgatcat agagtgttcg cttgttttta ataccatggt
     6181 atctcgccac gttcgatgca atttgccgtc gtgcttgtaa ctttggatcg tcacaaactc
     6241 tccttctttt ggaactccca tagcaaccct tccttaagca gcattttgtg ctttgtgttt
     6301 gggtgttcat tacaccgaca tcataattat tatatcaata aaaatgagct ttgcctagaa
     6361 aggattccta ttgtttcgat ttttctctaa ctgatctacc agtttcattt ggaccttagg
     6421 aaaaacgacc gaatcaaatg catcaggctt caaccacttc gaagttttgt tctcggtaaa
     6481 ttcttgttct gctccctcgg ttgcccgtcc gtaaaaaagg agaacatgcc atttcagatg
     6541 actaaaaata tgagtaactt ctcctaaatg tcgtgtctgc caaacaaaga gttcctgctt
     6601 ttcaaatggg agattttgct cttcttctgc tactaggtca tcaaacagat cgatttctgg
     6661 ctttgtttcc cattcttttt tcaatcgctc atactcctct tgtgtcactt ccatcattgg
     6721 aaatgtccac atattcgcca gcaatttttg gctatctctt tcttcaaaat aatacgctcc
     6781 agaatgattt tgaagagctg cactgatata ataaacgtct tttggctttg ctttttttgt
     6841 ttttacagga aaactcgttt ggattcctcg tttgttagct aaacaaaagg cttggatcgg
     6901 acaagtctca cactttggag aagttggtgt acagatagct gaacctagat ccatcattgc
     6961 ttggttgaat tcacctggat gcttttcatc aatgattttc cgcatcgctt catcaaagat
     7021 tttcctgcta gaagcttttg cgatgtctgc ttcaatgcaa aacaatctgc tgactactcg
     7081 catcacgttg ccgtcaactg caggttctgg aagtccaaat gcgatacttg caatcgcgcc
     7141 tgtcgtatat ggtcctattc ccttcaatga gctaatttct tcaggcgttt gcggcatttc
     7201 cccatcaaat tctgacatga tctgtttagc agcagcttgg atgttccggg ctcttgagta
     7261 ataaccaagg ccctcccatg cttttaaaag cttttcttct ggagcatttg ctagctcttc
     7321 aattgtagga aaccattcca tgaaacgata aaaataatcg atcactgtgt ctactctggt
     7381 ttgctgaagc atgatctcag agatccagat acgataagga tcacgattat agcgccaagg
     7441 caggtttctt ttttcttgtt cataccattg aatgaactga tcttgaaatt cttttgtttc
     7501 tttatctgtc catttttccc attctttatt cactttcctc ttccttttcc gtaatgaatc
     7561 gctggatcat atccaaatca aatccttttt gatataagct ttgctttact tttagatttc
     7621 tttttttagg ttcaaacctt aaatttcttc gccatagttt atctccttgt ttgactaaat
     7681 aatcatattc tatttcttga tctgcttctt gcggaagatc agctatcgct tgttggatca
     7741 cttcttgaga aaagcctttc gtcatcaatg tttgttggat ttttctcaat aattcttttt
     7801 ggctttttcc gtgatttttt ccaaacgctt tttcggctgt ctgatgtgca ttttgaactt
     7861 ggtcttcaaa tgtatattgg ctgagcgctt gttcaatgat ttcaggtttc aaaccttttt
     7921 gctgcatctg ctgcgctaat ttctttggtc ctttgtcact taagcgcata ttcgtgcgca
     7981 cataactttc cccatagacc aaatcatcga caaggtccaa ttcttttaag cgggctacga
     8041 ttttatttcg atcttcttga aggatttcct tttcttttaa gtatgttcgg atttcttttt
     8101 ccgatcgcaa ctgataactt aagtaattca ttgctaggtg aaaaccgaaa tcagctccgc
     8161 tactcttctt gatctcttgg atagtttctt cgtctaattc ccgtcctttt aataatcgat
     8221 agcgtactaa tacatcttca gatacttgta aagcttctcc ttcagataaa tctacgcgat
     8281 agaaagggcc tttgccttta ctgattctcg taacggttaa cataaacatc ctcctcgaac
     8341 atatattctc tatcatacca cagaaaatcc ccgtaatcag gtattatatg ttagaataaa
     8401 ccacagaaaa aagaaaaagg gagatactta tggcagaaac aattgtcaaa actggacaaa
     8461 cggttccttt gaaaatcaag cgtttaggca taaatggtga aggaatcggt tatttcaaac
     8521 ggttgatcat atttgttcca tatgcactac caaaagaaga agtactagtg aaaatcacga
     8581 aagcaactcc tcgctatgca gaaggagaat taataaaagt caaaaaaaca agcaaagatc
     8641 gcgtagtggc accatgtcct gtttactatg aatgcggtgg ctgtcaatta caacacttag
     8701 cttatcaagc tcaattggat tttaaaaagg atctgctttt gcaagcattg gaaaaattca
     8761 aaccagcagg ctttcgatct tatcaacttt tgccgacgat cgggatggat gagccttggc
     8821 attatagaaa caaagcacaa tttcagttac gaaaaaataa gcagactcaa aaaattgaag
     8881 ctggtttata cgaagcaaac tctcatcagt tagtccctat aacagactgc ttggttcaag
     8941 aacctcgaac acaaaaagta atgaatactg ttgttcaact attgaataaa tttgatgcac
     9001 ccatctatga tgaacggaca aacagtggaa tcttccgcac aatcatggtt agaatcggtg
     9061 ttaaaactgg ggagttacaa gttgttttta ttactcgcag taaaaaattc ccgcaaaaaa
     9121 acgcaatgat ccgtgagatc aatgaacgac tgcccgaagt tgtttcaatc atgcaaaatg
     9181 tccaggcaca aaaaacgtct ctcgtgatgg gggatgaaac gatccactta tggggaaaag
     9241 aagccattga agaacaaatc aatgaagtga ctttcgatct ttctcctcgt gccttctttc
     9301 aactcaatcc tcagcagacc gaattgcttt atggcgaagg aatcaaagca ttagatgtcc
     9361 agcctaatga aacgatcgtg gatgcttatt gcggagttgg gacgatcggc ttaagtgtag
     9421 caaaacaagc caaagaagta cggggaatgg ataccgttcc tcaagcaatc gctgatgcaa
     9481 aaaaaaatgc ggaaagatta ggatatcaca acactcgata tgaagtcggg acagcagagc
     9541 aagttctgcc taaatggttg aatgaaggtt ttcagcctga tggtatcatc gtagatccac
     9601 cacgtacggg tttggataac gcattgatcc aatcattatt gaaataccct ccaaaaaaac
     9661 ttgtttatat ctcctgtaat gtttctacac ttgccagaga tctggttaat ttaagtaaaa
     9721 agttcaagat tgaatattta cagtccgtcg acatgtttcc tcagactgca cgttgtgaag
     9781 ttgtcgtgaa gatgacgaga ctcgctgact aaatgaaaga ggttgtgaaa aagtctgccc
     9841 tgtatcaagt aataagaaca gccaaacaaa aatagctctt catgttttat gaatactgta
     9901 cttgtgaccg aagagttaac aggaggacac tatttattta aaaaaagggt tgtgacatag
     9961 tttttgtcac agcccttttt gattattatt ggatattcac gtctatcact tgataaaatg
    10021 catttgtcgt atcgctgata ttccatactg ctaaaatcac attatatcct tttcgatcct
    10081 gcggaagtgt gatcgtttgg ttgactgttt ttggtggaac agttgctttg tcgtctattt
    10141 ttgtgatcaa ttcaaaatca ctaaatttca aaggttgatt aggattccaa gttggtttgg
    10201 taatgtagta atcccaagta cttgtccgat gttgtgctgt taaattccaa gtaatattta
    10261 atgttcctgg attaaccatg tttttatgcc atctgttaga tgtctgttca tctaaaggct
    10321 caaagccgct gactcctgca ctagcaattt ttccatcaat gaatgtattt ttggtagctt
    10381 cgatactttg cggttcccat tgtgctcttc cgacattttg atttaagttt cctgctgctg
    10441 atgttcctag gtatgcacgg cttcctggag aagtaatata accatgcgca aaacctggaa
    10501 tgctaaaacc gaacaaccct aaactaacta acccaataaa caataacgta ctaatctttt
    10561 tcatgtagaa acctccttca tttgataacc acattgtaaa cgctttattg tattaacaca
    10621 aacattatgc ttttataatt ttatgttgaa gtaaaaaagg tgaaaaaagt aagatgcaga
    10681 ttataacaga agtagacgag aaaagtacaa atagaaaaca tgagaatcaa atcatatgat
    10741 ctttttcaaa aacatcattc atataaacaa atgttcaact ttttaataac aacaattatc
    10801 aatttttttc ttacttcaca aactaaatac aggtcttgct gtgagccttc atggtaaact
    10861 aatattatag aaaggagtta gtaaaatgaa gatgataaaa gatattcgtt tttgggtttg
    10921 tgttatagga attgtcatat taggcttttt atcagggttg cttagcggaa atcccggaaa
    10981 atattactat tctttgcaac ttccaccatt tgcaccacct agctggatat ttgggccgat
    11041 gtggacctta ctgtatatat tgatgggtat ttctctttat ttgttactta atcacaacaa
    11101 caaaaaacag cgaaacaatc ttgtcggtct ttttgtgatt cagttcattt ttaattttat
    11161 atggtctgct ctatttttta atttaagaaa tattttcatt gcagcaatcg atattacact
    11221 tttagttatt ttcttgtcag tgcttatgta tcagttgtgg ctgcatcatc gcctggcaat
    11281 gtggctcatg attccttatt atttatgggt actattcgca actctattga attattcaat
    11341 ttatttttta aactaagcgc taaaaagaga cgtcctgtaa tttaacacat agaaaaagtg
    11401 ccacctaagt tagatttt
//
LOCUS       DAYYKN010000089        11414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000089 DAYYKN010000000
VERSION     DAYYKN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.089"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..1492)
                     /gene="tyrP"
                     /locus_tag="ACHBB7_002001"
     CDS             complement(71..1492)
                     /gene="tyrP"
                     /locus_tag="ACHBB7_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM9209944.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1735..3612)
                     /gene="tdc"
                     /locus_tag="ACHBB7_002002"
     CDS             complement(1735..3612)
                     /gene="tdc"
                     /locus_tag="ACHBB7_002002"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM9209945.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3903..5159)
                     /gene="tyrS"
                     /locus_tag="ACHBB7_002003"
     CDS             complement(3903..5159)
                     /gene="tyrS"
                     /locus_tag="ACHBB7_002003"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM9209946.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5271..5476)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5739..7040
                     /locus_tag="ACHBB7_002004"
     CDS             5739..7040
                     /locus_tag="ACHBB7_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM9209947.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7109..7708)
                     /locus_tag="ACHBB7_002005"
     CDS             complement(7109..7708)
                     /locus_tag="ACHBB7_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9209948.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            7963..8844
                     /locus_tag="ACHBB7_002006"
     CDS             7963..8844
                     /locus_tag="ACHBB7_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9209949.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            8857..10467
                     /locus_tag="ACHBB7_002007"
     CDS             8857..10467
                     /locus_tag="ACHBB7_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9209950.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(10544..11203)
                     /locus_tag="ACHBB7_002008"
     CDS             complement(10544..11203)
                     /locus_tag="ACHBB7_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9209951.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
BASE COUNT         3328 a         2281 c         2076 g         3729 t
ORIGIN      
        1 aaaaaactat tcacttggca ggacctccta ctaaatcagc gacaaaacgt cggctgaaag
       61 acttattttt ttatttttct tctaaacttt tttctacctc aatcccccaa gaatctttct
      121 tgaagtgatt gatgatcaat ggaataacga acatgaccac tgtgatagct gctactaata
      181 atgcatcact caatgtcgct gtacctaatg tagcaaatac gacaacgatg atagagaaga
      241 tcagcatgca gctgactacc caagctaatc cgtttccgtt tttaccaatt cggtaaggac
      301 gttcgaattc tggatgtttc ttacgcattt taagcaatga aacagcaatc aaaatgtaca
      361 cgatacaata aataacagtt gtagcatttg taagtgtcaa gaatacagca cttacgtcat
      421 ccattacacc ataaagcaaa gcaaataatg aaataacgat tgattgtgtt agaacgacat
      481 ttctagatac gccgtatttg ttttctttat ggaatccgaa ttttggtgga aggaatcctt
      541 cacgagctac ttggatgatt gtttttgatg gtccagtaac ccaagctgat aattgtaaaa
      601 gcacaccaat aaataccatg aagctgaaga tatttccgat cacttctggt aagcctagga
      661 tttgacagtc aatcaagata ggctgcgtga tatttgctaa ttccattttt ccatctggta
      721 cgacatttgc tacaagcatc gcattgatga cattcaataa aactaatccg atcaaagcga
      781 taaatacgcc ttttgtataa ttttttgttg cgtctttcaa acgaggcatg taaacagaac
      841 tcatttcgat cccaacaaaa ataaatgtga taccagctaa gtatttcaat gaatcaatat
      901 gttccaagtc agggaaagct tttgaccatg aaaagtcacc tagatagccg tttgagacta
      961 gtccgacttt gaaggctgcc aaaacaccta aaacgaacat gatgactact gggatatata
     1021 cccctagcca tacaccgatg ttcccgccga cttttgccat atcgaatttc aagttcaaga
     1081 tcgtgatgat ccagtagata actaaaatac aacctaaaac gaaccagtga ttattgccaa
     1141 gttctacatt accaaatgtg tttcctaaca atggtcctaa agtagaagcg accattacca
     1201 tacctgggaa catttgcacc cataatagcc atgcaacgac aaatccccat ttgctgccca
     1261 gtgctgtttt tacccataac tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac
     1321 cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca
     1381 atgtccaacc gactgcggct aacgtaggaa tactccgaac cgttgcacac agtgccattg
     1441 tcagaccgat aaaggtccca agacccattt tacttacttc agatttactc accgttgaca
     1501 ctctccttga ttttttggat aaaataacgg tgtctttgaa gtagtttcag caataaacga
     1561 aacaattcag cgaatatttg aggggattct ttgagtttat tgcttgaagg gtttcttcaa
     1621 agacaacctc aaaacaaagg gatcgaagag cttaggtctc cccttgctct tcagatccaa
     1681 acatgcgtca gaaacagtta gtattcaagc tgttttctaa catgattacc ttgcttattt
     1741 tgcttcgctt gccaataatt ggtctgcgta gattttttcc aatttttctt gaagagctgc
     1801 tttgattttt tcagcatatt cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac
     1861 gctggcacgc aatacagtta cttttccggc acggttccat tcttcatctg agaatcctag
     1921 ttggttcaca aattgtaatg ggctgttgcc atagtctggg atagcaaagt cagtatgtga
     1981 agttaagaac tcattaccgt aaatgcttcc tttcacgtat gaagaataat catatacgtc
     2041 atggttcaat ttattcattg caactaagtc atcgttgcct ttttctttga atacatagtc
     2101 taccatattg aaatctggat aagttaatgg atgaacttcg atttctttgt ctcctacttt
     2161 aaatgacaag tcattcaaga agttgtagaa acgatgtgca ccttcgattg atgcacccat
     2221 taatttacca tatcctgtga cattcaaagg taagacatgg tgtgctgccc aaacgctggc
     2281 tgctgttgcc cctgcttttg aaccttccaa gatataagcc ccaagtaatg ctgggatatc
     2341 tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata acgtctctca tgcggatatc
     2401 tttgatgacg ataccaccgg cagaatatgg aacatagccc attttatgtg ggtcaatcgt
     2461 tacagattct gcttcttcga ttgctttata tgcactgtgt acttcttcta agatataatc
     2521 tttgttttct gtaaagacat tgtatttgta atgaacatct tttaaatctt caaatggaat
     2581 gaagttgttg tcttcgtcta agaaaatcgc acggccgtat ccgccataag cagcatctac
     2641 gtgtaagtag aagtaaatac cgtctttttc taatacgcga cgcaatgcaa cgattttatc
     2701 gataccgtcg attgctcctt cttctgttga tcctacaaca ccgactacac ctaggattgg
     2761 tgttttttca gctgctagac cacgaacgat tttttcaagt tcgttgatgt ccatgcgata
     2821 gttgtggtca actggcactg gaatcacttg gtctaaaccg ataccgatga tgtcggcagc
     2881 ttttagccat gaatagtgtt tagtttgtgg aaccaaccat tttcctaatt tttctaaatg
     2941 ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt ttttctggga cgctgtctaa
     3001 cagattcatg atttcttctg ttgacaagtt cattaattcc caatcgcttt ttccagcaac
     3061 tagttcaggt gtcacttctt tcattgcaag cggcaatgat ttgatgttgc gggcatacca
     3121 aagtccttct aagtttgcta aagaaccatc agcaacgatg tggccccagc catctttata
     3181 gctcattaat tttgcaaatt ccattcctac ttcttcttcc atttggcttg ttgcaggaga
     3241 agattcatag gcaacgttat taccattcca tagcatagca aaattgtacg ctagtaaaga
     3301 tggcatcaat gtttcagagt tcatatgacc ccagtaacgt ccggcattat gccaaggaac
     3361 ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca tcctttgttt tattcacagt
     3421 gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata ataggcatgt cttgaggcat
     3481 gtagttttga cgccagccta aatgttcatc caccaattca ttcaacaaag ctttatagat
     3541 ttggccgttc tcagctttgt ccccaatgaa tagggcattt aggtttagat ctttcgacaa
     3601 tgattcactc atagtgaaac cctcctaaaa aaatatttaa attttgtcga gccctacatt
     3661 acacctgttc ttctattctg taaactaagt tttggcctga tttttaatca tattttaata
     3721 tgtatcaatt atccgaattg ttcacacttt tgaaataatt aaaaaaaacg agggaattta
     3781 caaatgtaaa ctccctcgca aagattgtat taacgacaac cagcgttcta ttttaggatg
     3841 ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt caaggtaaaa acttactaaa
     3901 ttctaatcaa ttactttggc caaaaagtaa tttttcttac cttttcgaat aaccacaaac
     3961 ttaccatcga attcatcgga tggatttatg acaaaattta aatcggtaac gcggtcaccg
     4021 ttaatactta tagctccgtt tgaaacatct tcacgtgctt gacgtttaga tggttcaatt
     4081 tttgtggaaa caagcagttc cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc
     4141 attttaccga atccttgcgc gatttcttcc gcatttagtt ctttaatatt tccagagaac
     4201 aatgcttgcg ttattttttg cgcttctttc aagtcttctt cactatgcac aaatcttgta
     4261 acttcttctg ccaaacggcg ttgtgcttca cgtttctcag gttctgtctc tacttttttc
     4321 gctaagtctt cgatttcttc ttgagacaag aaagtaaaga atttcaagta tttcactaca
     4381 tcgcgatcat cttgattcaa ccagaattgg tagaactcaa atggtgaagt tttcttcgga
     4441 tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc
     4501 aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt ctaattttcg gattaggtct
     4561 aatccagctg tgatatttcc ccattggtct gctccaccga tttgcaattg cacatcgtat
     4621 gttttatgca gatgcaaaaa gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat
     4681 gagattccta cttccaaacg gctggcaacg atatcttttg ctaacatcgt gttgatgttg
     4741 aagtttttac cgtagtctct caaaaattca agtaaagaga tttttgacaa ccagtcgtag
     4801 ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt ttctcatttg attggaaagt
     4861 gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca cacgttctgt tttgcggccg
     4921 cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa tatatggatg atggcctgcc
     4981 aattggaatc ttttcatcat cataaatgga atcaaatgtc cgatatgcat cgaatcacca
     5041 gttggatcaa cgccgcaata aagtgaaatg ctcttttctt caacgagctc gcgaagtctt
     5101 tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta attcatcaat aatattcatt
     5161 ttactgacct ccgtgtagtt tttttatgaa agcaggaacc tgctagcatg tgtaattaat
     5221 tatttcttca ggataggata ggaaataaaa aaagtccctt ggcatataag ccaagggacg
     5281 aaatctcgcg gtaccaccca atttgtatgt ctagatatcc aacatacctc tcgaacattt
     5341 aacgctgtcg cgtggcaatc actgccaaag actcctgaaa tgtaattcgc tttaaagcat
     5401 gtactggttt gcagcgacca ccagctttct gaaacattgg ctttaacaac tacttcgttt
     5461 cgatcttcgt cttgttatta ttaatatgtg tctatcatag agcgcaactt tttctttgtc
     5521 aacagaaaaa ggatattttt tttaaattcc tcattttttc ttttttgaat ccgctctaat
     5581 tccgaacatt ttattttcaa ttattgttaa aagaaataac tttttctgta ttttttctgc
     5641 aatttatcga gaagtcactt tactttgcct cttattcttt cttcgttaca ataaggaatg
     5701 ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta
     5761 ttttaggagc cacttctgct ttaactatcg ttattttaat tctaagtttg ttttttgtca
     5821 tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa
     5881 aagaaccgac agatgataag caacaactag ttactcaagc aaaaaatctg gctcaaagct
     5941 acgattatga ccaagcgatt gcgttattgg aaaaagatag taagaacgga tctactgaaa
     6001 cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca actgatcgac tggtctgatc
     6061 ctactcagat ctctcatgtc tttttccaca gcttgatcgt ggacccgcaa aaagccttcc
     6121 actctcaaca ggcacaagga tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt
     6181 cgatcgaaca actttatcaa aatgattttg ttttagttaa tttgaatgat ttgatccaaa
     6241 aagacgaaca aggaaatctt tcctttaacg ggcttgcgct acctgaagga aaaaaaccat
     6301 tggttctttc acaagatgat gtcagctatt atgaatatat ggataattcc ggttttccta
     6361 gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt ctataaagag aaagataaag
     6421 aagaaatcgg tgactttgac atggttcctc tgatcgatgc ctttgtcaag aaacatcctg
     6481 atttttctta tcgaggcgca aaaggtacct tggccctgac aggttacaac ggagttttag
     6541 gttatcgaac atcaaaatct caatatggag acaatgaaaa aacaaaaaaa gagattaaag
     6601 aagctaaaaa agtggcggaa cagttgaaga aagacggctg gagctttgct tcccatacat
     6661 ggggtcactt gaatatgact caagcttcac tagaagatgt aaaaaaagac aatgaattgt
     6721 ggcagcaaga agttgcacct atcctaggaa aaacaaatat tctgatttat ccatttggtg
     6781 cagacatcag cgactggcag ccttattcag cagaaaatca aaaatttgct tacttgaaga
     6841 gtcaaggttt cgatattttc tgtaatgtcg atgcttctac accagcttgg ggacaattag
     6901 gtacggattt ctatcgaaat gctcggatca atatcgatgg cattcgtttt caatctgatt
     6961 tagacggaaa aaatcctatt cttgatccat tcatcaatgt ccatgatgtc tatgacaaag
     7021 aagcacgcga atctgcttga ttttacgtca tttatgaacc aaaaaagccg aactgacatt
     7081 ttatcgtcag tttcggcttt tttatgcttt atttcttctt cggtgcgatt cccttgatca
     7141 ggacttctgt ccattctgct gtatatggaa gcagttttgc gtatatctcc ggcttagaaa
     7201 ttccttctac cattgcttga aaatagagga tcaagaactc atccgaatat ttcaaatcca
     7261 cttttccttc tttccgccct cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg
     7321 tatacgcatc catcgcgtca gaaaattctt ttttttcttt ttttgtatag tatgtcatca
     7381 aatcgatata aaattgtttg ttcactcgat tgagataacg gattttgttt tcactcatgg
     7441 caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta
     7501 aatcattcat aaatgcaaaa aatacttcat aaatcagccc ttccttgctg ccaaaatatt
     7561 taaaaatcgt cgctttactt acctgagcgt tctcagctac cttatccatt gtcactccag
     7621 cggttcctat gtctgtgttc atcaagtcaa aagctgcttg taaaatctct ttctttttct
     7681 cttgtgctct cttttcaaaa ccgttcattt gtagagcctc ccgtaatttt aaacttttat
     7741 aatatatata gttcaaattt attctgaggt ttattataac tatctattga gaaagtatca
     7801 agctagcaat tccttgttta tgaattcatt gacagaaatt tataaaaaga atattatgta
     7861 ctaaaataat aaaaaaaagt tttataaaaa ggtataaaac ctttttatta aactatttaa
     7921 tcttgtttag tatatatttt tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa
     7981 gtcacacatt tgaaaaagag ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc
     8041 gtacattcag gtgaagtcgt tggatttatc ggaccaaatg gttctggaaa atccacgacg
     8101 attcggacac ttttagggat tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa
     8161 gatgtatggg aagatagttt atctatccat gaacgtcttt cttatgttcc aggcgatatc
     8221 tctttgtggg gaaatttgac tggaggagcg atcatcgatc tcttcatgaa gcttcatgga
     8281 aaagggagtg ctcaaaaaaa agatgaactg attcaacgtt tctctcttga tcctaagaaa
     8341 aaagcaaaaa actattcaaa aggaaatcgc caaaaagtgg gcttgattgc tgctttggct
     8401 gttgattcgg atctctattt atttgatgag cctacttctg gtttagatcc tttgatggaa
     8461 acgattttcc aagaagaagt tgaaaaattg aaagaacagg gcaaatccat cctactttct
     8521 tctcatattc tcagtgaagt cgagcgacta gcggataaag tcgtgatcat acgcgaagga
     8581 acactcgatg aagaaggaac actcgatgaa ttgcgtcatt tgactcgttc gaccatcact
     8641 atagaaacag aagcagagaa tagtcactta gcacaattga acggtgtcca tgactttgtg
     8701 caaaaaggtc acctcgctca atttgctgct gataacgaag ctttcgatgt gattttagct
     8761 gaagcgtcca aattaggtgt gaaaaaattt gaatctattc caccgactct tgaggattta
     8821 ttcatgagac attatgaagg atgaggagga agcgaaatga ctgataaatt ttcacgctgg
     8881 tccacccttt tttctcaaaa ccttagccgt gactggaaaa agatcctgat atggctagtc
     8941 ggtgtcactg ctttttctgg tggttttgtc cctgcgtttg aagaattgac ttccggacaa
     9001 ggattagctg gattatacga aaccttgcaa aacccggcaa tgattgcaat ggtcggacca
     9061 actccagctt cgaatgctgc tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg
     9121 cttttttgca gtttgtttgc tgctgtgatc tctgttttac acgtggtgag ccaaacgaga
     9181 aaaaaagaag aacttggtct gattgaactt attcgttcct ttcgagtcgg acgtcaagca
     9241 aactctttag ctgtcatcgc tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc
     9301 agctgtacga tgattttttt ccaagcggat tcgatcactg cagaaggtgc ttggcttttt
     9361 gggctatctg taggtagtat ggggattttg gggagtgccg ttgcactgtt tgctgcacaa
     9421 ctgatgccaa gttcagccag ttcgaatggt ctgtctttag cgtttttagg tggactttac
     9481 cttctacgag caataacaga tgtagctgat tcctcccttt cgatgtggaa tcctttaggc
     9541 tggtcttatt tgacctatcc atttacaact aacaactgga ttcctttgct ttatttgttt
     9601 ttgttcagtc taatcttgat aatcagttca tttgttttag aggaacatcg agatttgaac
     9661 accggctata tccccgaacg tgaaggaaaa ccgtatgcta aacgttctct tttatccttg
     9721 cgtggtttat tattctatct aaataaaggg atgacaatca gttggcttat cacttttggt
     9781 ttgatggggg cggcatacgg ctctatctac gggaatatgc aacagttttt agaaagtaac
     9841 gacttaatga aacagatgtt tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc
     9901 acgatcatgg tagtcttgat ttgtctagct gctattttac caattgctag catcaatcgt
     9961 ttatttacag aagaaaccca cggacgtatg aatcagctgt tcgcaacaaa aatttctagg
    10021 aaccaacttt attggaccaa tatgttacta gctattctat ttgctatatt ggcaatcctg
    10081 attagttctg gtgcactagg gctttctgct ttggcttcga tgaaggatgc gaaagacatg
    10141 gatctattga tgtttcttaa agccgggttc aatttcctgc ctgccgtttt agtgtttatc
    10201 ggaattagtg gctttctatt cggatggctt cctcgaatcg gtaaacttgc ttatatctac
    10261 ttagcttact cctttgcact caattacttt ggcggaattt tggatttacc agaatggttt
    10321 tccaaaacag ctgtccctag ctgggttcca cggatgccaa acgattcttt tgatctttcc
    10381 gtgttcctta cgttatcatg tatcagcctt gcactgttcg tccttggttc tattggttat
    10441 aggcgaagag acttactaga aggataataa aaaagcatgt acaatttcat gctagcctcc
    10501 aaatctgggt caaaaatcca acatttggag gtcatttttt tatttacaag tcttctaact
    10561 gctctttatc aatgatccag acttctcgat tctttctttt tacataacca ttctcctcta
    10621 ataatttaaa ttttctagat aaagtttcgg gggtcgtacc gataaatgca gcaagttctt
    10681 tcattttcat tggcaggaaa aagtgatcct tccctgtcgc tttagctaag tcaagcagat
    10741 agacagcgac tcgttcttct actttttcca tcatcaaaaa agttgtctgc tgctgtactt
    10801 ttgttaattt ctcaacgttg attgctaaaa gtttcagact aagggcggga taatgcagta
    10861 agagttccga gaaatctttt tgtctcaata tgcaaatttc cgttttttct agtgcttcgc
    10921 caaataaaga ctcatttgga acgcctagca actggctctc tccctcatat ccgcccggct
    10981 ctattactcg taaaagctgt tcctttccgc tagcagataa ttgatacact ttcatactgc
    11041 cggaggccac gatcactaat tgaggttctc cattcggcgc aagaatctgt tctccttttt
    11101 ccacatagtg atgtttgaca agtgcgtgta ctttttcttg atcttgctgt actaagtgat
    11161 tgaacaaagg aacaaatgaa acacataaat gatcatgact cataatctct cctccttcta
    11221 aacagctatt tctaataaag aaacataact tcccttattc caactgataa ttttctttta
    11281 tcatagcata aacgtcaagc gaaccagata ccagcaaata gagtggattt ctttttgtcc
    11341 cttgctttat tttccttctt ttgagacctt aagtaattga ttcctatcaa ttttttaaat
    11401 atttactttt tgaa
//
LOCUS       DAYYKN010000090        11355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000090 DAYYKN010000000
VERSION     DAYYKN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.090"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..706
                     /locus_tag="ACHBB7_002009"
     CDS             107..706
                     /locus_tag="ACHBB7_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFM9209952.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
     gene            706..1281
                     /locus_tag="ACHBB7_002010"
     CDS             706..1281
                     /locus_tag="ACHBB7_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFM9209953.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            1306..4977
                     /gene="brxC"
                     /locus_tag="ACHBB7_002011"
     CDS             1306..4977
                     /gene="brxC"
                     /locus_tag="ACHBB7_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFM9209954.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            5041..8673
                     /gene="pglX"
                     /locus_tag="ACHBB7_002012"
     CDS             5041..8673
                     /gene="pglX"
                     /locus_tag="ACHBB7_002012"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFM9209955.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            8789..11311
                     /gene="pglZ"
                     /locus_tag="ACHBB7_002013"
     CDS             8789..11311
                     /gene="pglZ"
                     /locus_tag="ACHBB7_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFM9209956.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
BASE COUNT         4267 a         1531 c         2141 g         3416 t
ORIGIN      
        1 ctataataaa tatgtattgt atagtagata ctaaaaaatg aatgctaacg atgagcaaaa
       61 aacacttgtt tacttattga attatagatg attaggaggc aatctattga ataaaaaata
      121 ttctgctggt ctagtttcta aacgcttttg gttttcagag ttcaagttat atattcaatt
      181 acgactagaa ggatatacag atatagaaat taaagagaaa aatgaacatg aaaatatttt
      241 ttttgcagtg agcagtaacc gtcaaaaaga aatctatcag aacgtgaaaa ggagagttag
      301 tcaactggat gaagctggat tgcaactttt caatcagtta aatatagata atcaaaagtt
      361 agtgaatttg atttccatcc ttatgattga tgatcttttt aatgaattta tgactgaggt
      421 atatgctgaa aatctacgta aaggaaattt gcaattaaca ccagttgatt accgttcttt
      481 ttttacagag aagcggagaa gttcagaaga tatttcaaaa tggaaagatt atacgattaa
      541 acggcttatt gccgcttatc aaacgaatct aattgaaaca ggattagtca gaggggaaaa
      601 agaatatgga cgaatgactc cgatattatt agataaacga ttgattcaat ggttagttga
      661 caagaatcgt aaagatattg tactagcact gggaggaatt ttataatgtc attatcgatt
      721 caagagcatt tggaaaagtt gaatgaagta attcaaaaag aagatttttt attgaatcgt
      781 ggtttaggta atgaagtagg ctattatatc tttgactacg atccgaaaga cgagttaatt
      841 gtaagagatt atattaatga tatatctgga acatctgttg gtgataatta tgaagtgaaa
      901 gtttataatc tctatgtgat tatgttagag tatctagaaa aacgcggttt cactgaaaaa
      961 gcgattaaga tggaagaacg acgtggttta caaaaattag tggaaagcat tggtcgttta
     1021 ttgaaaatgg atttacctga ggaagaaaat gtattttttg aatatgtgga tgcccataca
     1081 ccagaaaaat cagttgtgtt cctgacagga attggcgaat tatatccatt aattagagcg
     1141 cactctattt taaataaaat gcatcaggta tttgatcgcg taccagttgt gatgatgtat
     1201 ccaggggaat atgatgaatt aagtttgaca attcttaata ccaataaaga tgataattat
     1261 tatcgagcat ttagaattta gttgggatgg aaggtaggag attaaatgct aattaaagat
     1321 atttttgcaa aaccaattga ccgaaatatt caaggagtta ttaaggtcgg tcaagcaaag
     1381 gatgaaaatg ttcaacaaga attagaagaa tacgtggtta cgaaagaact acaaaaacac
     1441 tttaaaacaa tttttgatgc ctaccaacgg agtatcaata caccaacaga taaaatgggt
     1501 gtttggattc aggggttctt tggttcaggt aaatctcatt tcttaaaaat tatttcttac
     1561 ttgctcgcaa atcgagaaat aaatgacaag aaagccattg attacttcaa agaagatggg
     1621 aaaatcaaag atccactgac tttggcaacg atggaacggt gctcacaaat aaatacagat
     1681 gttattctgt ttaatattga ttcaaaaagt gatgcaggtg gtaaaaaaga caaaaacgga
     1741 atcgtgaatg tttttttaaa ggtatttaac gaaatgcaag gattaagcac gattccacat
     1801 ctagcagatt ttgaacgtcg gttaattaaa gaagaaaagt ttgatgcatt caaagctgct
     1861 tttgaagaga ttaatggggc aaaatggcaa gaagagcgcc atgagtttga ttggatccaa
     1921 gatgatatca aagaagcggc acttaaaatc gattttatga gtgaagccgc tattgataat
     1981 tttttagaaa aagcatcagg aaattattta ataagcattg atgactttgc aaatctagtt
     2041 aaaagctatc ttgattccaa agaagcagac catcatattg cctttctagt cgatgaggtc
     2101 ggacaatata tcggcggtaa tcgtgagttg actttgcagt tacaaaccgt tgtggaagat
     2161 ttgggtcgaa tttgtgaagg taaggcttgg gtattagtga caagtcagga agcgattgat
     2221 cacgtaacaa actttgataa caattctgat aatgattttt ctaagatcaa ggatcgtttt
     2281 gccacaactg tgagtttatc ttcagcaaat gccgatgaag taattaaaga acgtattttg
     2341 aagaaaaaag agtttgccaa tgattcttta gccttacaat ttgaaactgt tcaaacagaa
     2401 ttgaaaaata aattactctt tgttgatagt cctgaattaa agctcttcca agatgctgaa
     2461 aattacgcag aagtttatcc atttattccc tatcaattca atttattggg gcaaattcta
     2521 accgagattc gtaatcacag ctatcaagga tcaaacttat cgtctggtga acgttcatta
     2581 ctagcaatgt ttaaagaagc cgctgaacga aataaggaaa atgatacaac cagtttggtt
     2641 tctttagatc agttttatta ctcgttagaa cgttggattg atacagcaat tagccgagtg
     2701 attacacaag ctacaaataa ttctcatatt caaagaccaa ctgatataga taactttaac
     2761 gtaaatgtct tacgaatttt attcatgatt aaatatgttg atcaagccat caaaccaaat
     2821 gttgaaaata ttacgagctt gatgataaga aatatcaacg aagaccgcct ggaaactaaa
     2881 aaacgagtag aagaagcatt ggacctttta attaaggaaa actttgtgcg gaagaactta
     2941 gatagttata ttttccaaac tgatgaagaa caggaaatta ctcgtgagat taaaaatcaa
     3001 aatattacag atagtgattt gatcgtagaa ctatctaaac gaatttttga cggcgttcta
     3061 gaccgaaata gttttgccta tgaaaaatta ggcatgtcag gtggaaataa atttaaaaac
     3121 cggtatacgt ttacttatgc acaagtgtta gatcaacgtc catacaagca agctggaaat
     3181 gctgaaatga cagttaattt tatttcacca tactctgatt tagcaggtca agaggcagaa
     3241 cttgcgctta agagttcccg taacttatta ttcgtagaat tgccatcaga taaagaattt
     3301 aaagatgata tagcaatgta tgctagaatg aatctttatt taacaacgac ttattcgagt
     3361 gtagtaaaga actttgacgc gattcgttcg gaaaaatctg gtgaattaca ggaactaagt
     3421 gcaagtatca acgatcaatt aaatgaactg ttgaaatctg caaaattttt ctataacggt
     3481 gcagaaattc aactgacagg ctccgacttt aaagcaaaat tccaatcagc acttcagaaa
     3541 atggctgaag aagtatacca taaattaaac caaattgatg cgcctaaaga aaaagaagat
     3601 atcttatatt tagtgaaaga aagcaaaaat gaaattattg aagatccatc aaatgagcaa
     3661 gcagttcagg aaattttcaa ttttatcaat gatcaaaaga gaaatcacct aacgattacg
     3721 atgaaaacaa ttcgtgatcg ttttgaaaca aaacaaccgt atggattcac agaacttgat
     3781 gtggaatggt gcatcgctaa acttttcatg tctggtaaaa ttgatttact ttataacaat
     3841 gtacaaattt cacggatgga tgatccaaat agtattgtaa tgattattca aaaacgacga
     3901 gaaagtgaaa agataaaaat tgaaccaagc gcggtgattt cgccaaaagc aaaaaaattg
     3961 gttgacgaaa tcggtagaga actgtttcaa agtcgtaata ttgttgatga aaataacaat
     4021 gaacatacaa tggaaaattt caagaaatat ttaaaaggtc aattagacaa tttacgctct
     4081 tattttgatt tacaaaacac ttatacctac cctagtgctt atgaatatga gcaaataaaa
     4141 aaatatcttg aagtaattgt tcaaacgtca gatgttgaag acttttttta tacaatcaaa
     4201 aaatatcgcg atgaattatt cgactgggct gaaacatatg ataaaattaa aaatttccac
     4261 aagcaaaata gtactcagaa aaaaatctgg gagaatgcac aagataaaat tagattaatc
     4321 gaaaaaagtc ggcgtcaaac ttctagccaa gaaattgatc aattgattca tgagatgaaa
     4381 accattttga aaaaggctga tccttattca gatatgatga aactaaatga gttgattcaa
     4441 gaatttattg aacaatataa tcaactgtta gagttggaaa gtcaggaaat attaaataaa
     4501 ttaacggaag aaaaacaagc aacatttaaa ttattagaag gaaaagattt taaggatcaa
     4561 tttgtaccaa tagttgacca agaatttcaa gaactgaaag caaaaattga acaagcgaat
     4621 actatagatg atcttgtttt cttatcatta aacgtagcag aacagtattt gatctttaat
     4681 caacgtttcg ataatgaaga aaaccgcaga cgtcaagaag aacaagcacg taaacaaatt
     4741 gaagcagcaa acttgattcg tgaaactgaa aaagatgaaa tagactctgc agcaaaagct
     4801 gaagaaatag cgatagtaaa acctactatt caacagaaaa cagtaccaat gagaacactt
     4861 aacgttcatg ttaaaactat taagtcagca gcagatattg atgagttaac agctgaaatt
     4921 aatcaaaaac tgaaggaaca acttcaagaa aatacagaaa tcacgattca attctagtga
     4981 aaagacaggc ataaaaaggt gcctgtcttt tcaaaggtta catatagaag ggaaagaaaa
     5041 atggataaga aagcgataaa gaatttcgcg gtcaatgccc gtagaaaatt aattgatgaa
     5101 gtaatgacta aagcaaaacg tttaggaatt gatgaatcaa aaattgaaga tgtgaagaaa
     5161 attgatagca atttacaaga aatcgaaaaa tcaggaataa gaattcaagg aaaagaaata
     5221 gcgcaaagaa acaaattgat tgcagaacta aaacaacgtg cttctgtcac ttcttattca
     5281 gaagcttttc aagagttaat cgaagaggtt gcttatacgt ggtttaaccg attgattgcc
     5341 attcggttta tggaggtaaa tgattattta cctgacacgt atcgtgtatt atctagtgaa
     5401 ataagtggga aaattgagcc agatattatt acagatattt atgatgccga tatctttgaa
     5461 gaattatcta gtaatgaaca gcaacaagtc agaacttgga aaacagataa ttcagcagaa
     5521 gcaatggaca agctgtatca attgattttt ataaaaatct gtaatcaatt gaatgattac
     5581 ttacctgagc tatttgaaga agtagaagat tatacagaac ttttgtttac agcttcttat
     5641 attaaaaaag atggtgtaat cgctgatctt ttaagtattc cagaggatga tttctatgtt
     5701 caagctggtg gacaggtaga gattattggg tggttgtatc agtactataa tagtgagcca
     5761 cataatcaag tggtgaatat ttataaaggg attgtcaaaa aacaagatat tccggcagca
     5821 actcaactat ttaccacaga ttgggtcgtt cgttatatgg ttgacaatag tttaggaaag
     5881 tattggttag atcattatcc caatagtgaa ttaagaaata agttgaaata cttaattcca
     5941 gataaatatg cttttgaaca agcaacatct gtaagtaatt taaaaccaga agaactaaaa
     6001 attatcgata acgcaatggg gtctgggcat attttagttt acgccttcga tgtattgatg
     6061 gaaatttatc ttacgttagg atattctgag cgtgatgctg cacgtgcaat cgttgaatac
     6121 aatttatatg gtcttgaaat cgataaaaga gcgtatcaat tgtcttattt tgcaattatg
     6181 atgaaagggc gtcaatacga tcgtcttttt ttgaaaaaaa gtctatcgcc gaatttaaga
     6241 gagtttattg attctcctaa agtgacagac gagtatcaag agcgtattag tgaattgaac
     6301 aatgataagt atagtgaacg ctggaatgaa tacaaagagt taatttttga tcaatttgaa
     6361 aatgggaaag aacttggatc aatcattaag ttagtcgata aaaataatca ggcaataact
     6421 aaagaatact taggaaaagt aagagagtat atatcaaaat tcaacgaatt tacgaatatg
     6481 gatattcttt atgggatgaa ggaaatacaa gaaaaatact tgcacattct tgacgtggct
     6541 gaaatgctag ttgataaata tgtagcggta gtcactaatc cgccttatct taataaaatg
     6601 gataagaaat taaaaaaata tgtaaatgat aactattctg atgtcaaaaa agatctattt
     6661 tctgtgttta ttaaattgaa tagccaaatg ctgattgata atggatatgc ttcctttatg
     6721 acgccatttg tttggatgtt catttctagc tatgaaaagt tacgctcatt tttaattaat
     6781 gaaagaaaca tttctagttt gattcaaatg gagtattcag cttttgaaga agcaaccgtt
     6841 ccaatttgta cgtttacgat aaaaaattca aatgaacaag atggaagcta tataaaatta
     6901 tcagatttta aaggtggaat ggaagttcaa aaaattaaaa cgttagaagc aattcatcag
     6961 catggtgaat gcgattattt ttatagtact aatcaagaca acttcaaaaa aatcccagga
     7021 atgccgattg cttactgggc aagcgataac gtaatcactg attttgctca aggaaaatta
     7081 actaacgatg ttgcagatgc aaaacaaggg ttggcaaccg gaaataacaa taaatttttg
     7141 cggtattggt ttgaagtaga atttaacaaa ataaagtttg atgctaaaac attagaggac
     7201 gctaaaaagt ctagaaaaaa atggtttcct acaactaaag gtggtcgttt tagaaaatgg
     7261 tatggaaaca atgaatacgt ggttaactgg gagaatgatg gagatgaaat aaaaaattat
     7321 gttaatgaaa atgggaagtt aaaatctaga ccacaaaatc ttaattacta ttttaaaaag
     7381 ggaattactt ggtcgacact ttcatctgga caagtatctt ttagattgca gaatcaggca
     7441 atttttgaaa ctaaaggatc tgttctgttc tcaaaggata taactgactt tgaagaaata
     7501 tttggctatg taaactctaa attagttaat cactttttaa cttttatttc tcccaccttg
     7561 gattaccatg aaggaccaat gggaaaacta ccttttctta aagttacatc tgagagaaaa
     7621 ttaaaaaaaa taagagaaaa agtggatgaa aatatcgata tagctagaaa agactataat
     7681 gctcttgaaa cgtcatacga gtttagaaga aacattttgt taagttatat gtttaaaaaa
     7741 gatctctcag aggcatttaa ctgtctaagt aggcagttgg ttaatcaatt taatactcta
     7801 aaatcgaatg aagaggaact gaacgacata ttcattaatc attattctct tgaaaaagaa
     7861 ttaacaaaag aggttcaaga tgaatatatc tctgcaaggt tacctgataa ggcgagggaa
     7921 attaaggatt ttatttctta ttcaataggt tgtatcttcg gtcgttactc cctagacgaa
     7981 gatggtctca tcttcgctgg cggcgaatgg gatccttccc gctatcaaaa atttaaacct
     8041 gatactgata atgttttact catcaccgat gatgagtatt tcaaggatag ccaaattgac
     8101 attgtcaatc gtttcattga tttcgttgag attacttttg gtaaagaaac ccttgaagaa
     8161 aatctccagt ttatcgctga cactttaggt ggcaaaggga caagtcgaga aattattcgt
     8221 cgttacttcg tacaagattt ttacaaagac catttaaaaa catactccaa tcgaccaatt
     8281 tattggcaat tggactcagg aaaacaaaat ggctttaaag cgttaatgta cttgcatcgc
     8341 tatacaccag atcaattagg aaaagtacgt actgaatacc tacacgaact acaaaaagcg
     8401 tatgacaatc gaatcgaatt gcgtcagcaa caaatttcaa caagtgataa cccaaaagaa
     8461 agaaatgcaa tgacaaaaga aattgtaaag attcagaagc aattaaaaga agttcgtgag
     8521 tatgacgaga agttgggtca cttagcatta aaacgaattg aattagactt agatgatggt
     8581 gtaattgtca attatgataa gttgcagcgt gatcctaaaa ccggcgagaa attccaaatt
     8641 tttgcaaaag gggtcaaaga accaaaaaaa tagttatcta agaaagttgt tttctaacaa
     8701 ctttcttttt tattcttaaa ttgcgtgcga aattatccaa attatgtcaa tataaggggt
     8761 atagaaagag aacgattagg tgagattgat gacagatata aatacgcaac aaattgaaga
     8821 aaaattaaat caacgttttc ctaattatgg atgtaggaaa atcatttttt ggtttgattc
     8881 aaatcaggat tttcttgaag aaattgactc attggtcatt caaaatgcaa aaatctatcg
     8941 attagaaaag gacgcacaat ttaaaactaa gcgtttattg gaatatgaag atcccgaaac
     9001 taactattta atctatgcgc catttcctcg ttgggatgat aaagatgaag ataatcattt
     9061 gttgagtgta ttgaattatt cagaggaatt tagtgcagac cgcttagcga ttatcatgaa
     9121 tgaattggct attccactta gatttcatca gacagttaaa cagtatcgta atttctttaa
     9181 tgcaaaggat cgcatgagaa gttttggtga atttagtacg catatgacaa ttaataaatc
     9241 agaaacgatt gaattaatga taatggctgt tttgttgaaa tcgaaaagta ttcgattaaa
     9301 tgatttactg cgtgtagttc taaaaagctt tacagaaagt agggagaatc ctttaaggga
     9361 acttgataag tatcagttaa cagctaaatt ttgggaattt attcaaatga aatttggctt
     9421 tacggatagc cagccaacat tggaaaagct gacgatgtgt ctttatttga actattttta
     9481 ttatcaaatt gatcaagaga tgccgaagaa gttcaaagga tttgaaatac tacatttgaa
     9541 agcaaacatt attactttca tggatcagtt tatgaatgat acacgttaca tggaaagctt
     9601 tgaacagcta tctaaaaaag tatatcaggc tattgatggt gatggattat tagacagtat
     9661 agaaattgat gatctaattg aagcagacgt attttctgag attcatgcaa aaatattaat
     9721 gttttatgca gaacgattaa aggcttctga cactacaacg aaaattgcag gacaaacaat
     9781 ttttgatagt ttaaagcagc gcgaaaaaat gcatttcgca tcggattata tttatgagta
     9841 tcaattattg aaacatgctt attatcttgt ggataaaaac agattgtgct tgccgatgaa
     9901 catggataat ttgctaactc aatatgagaa ggaaatgtat tgggttgata cgcattatcg
     9961 gaaatttatt tggaatagtg atcatataga agaaaagcaa aaatatggcc aattgtatcg
    10021 attagttgaa gcaaattatt cgaagtttct tgatcaatca ggacaaattt ggaatcaaac
    10081 ttttgaattt aagaatcgtc catccattcg aaaattttat aatcaagctg taatgaaacg
    10141 ctctgttaaa actgtagtta ttatttcaga tgcttttcga tatgagttag ggatgaattt
    10201 agaacgtaag ttagttagag aaaagaagta ttcaacaaac atggctagct ttctttctgt
    10261 attaccttca gttactgaat tcggtaaagc cgccttatta ccaaacaatc aactaactta
    10321 caatgaagga acagaagtac tgattgatgg gaaaaaaacg aatggtttat ccaatcgaaa
    10381 tgcgattctc cagcaacgag atgcaaatgc tgtagcttta aattatgatg atgtggttaa
    10441 gatgaataag gctgaggtac gagaagtatt taatggcaaa tcagtcattt atatttatca
    10501 cgatcaagtg gatagaacag gtgatcactc gaatgaaaat cgagtttttg aggcctgtga
    10561 tttagcgata agcgaaattt cccgacttat taatcgcttg cataatgatg gcagtatcta
    10621 tcgttttata gttacggctg atcatggatt tatttataaa agaagccacg ttgaagaagt
    10681 agataagatt gaaaatccat ctagaaatga aaaagatcgt gtagaacgtc gttttattct
    10741 tagtgaagaa gaatacgaaa taattggtac gaaaaattac tctttgggag attctttagg
    10801 aaatgatgac actagaaaca tttgtgtccc gattacttca agtattttta agaaagctgg
    10861 cgggggacaa agctacgttc atggagggtc atctattcaa gaaatgctag tccctgttct
    10921 agaaattacg gtagccaaag gtagcaatgc aaaggaacca gtagcaattg aattaatgac
    10981 tagcaaccgc agaattacag gattatcaac cacgttagaa ttctatcaaa aaaatgcaat
    11041 tagtgatgtt tatgaccccg catctttctg gctttatttt gaagatgaat atggtgaacg
    11101 tatctcaaat gaagaaatct atgttgcgga ttcaaaaaca gaagatgcaa atcagcggtt
    11161 tactaaattt gtttttgaat ttgtaaatcg tcgatataca gttgaagata cttattattt
    11221 agtgataaaa aatcaaaaaa atgatttgac cgagcgaata aaattcatta tagataatcc
    11281 ttttgcagaa tcatttgatt ttgatatata aaaacgaggt atccacaatt ttttgtggat
    11341 actttttctt ttgta
//
LOCUS       DAYYKN010000091        11217 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000091 DAYYKN010000000
VERSION     DAYYKN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.091"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHBB7_002014"
     CDS             6..305
                     /locus_tag="ACHBB7_002014"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9209957.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHBB7_002015"
     CDS             378..1739
                     /locus_tag="ACHBB7_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM9209958.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHBB7_002016"
     CDS             1761..2021
                     /locus_tag="ACHBB7_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209959.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHBB7_002017"
     CDS             2035..2787
                     /locus_tag="ACHBB7_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM9209960.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHBB7_002018"
     CDS             complement(2881..3486)
                     /locus_tag="ACHBB7_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9209961.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHBB7_002019"
     CDS             complement(3539..4513)
                     /locus_tag="ACHBB7_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM9209962.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHBB7_002020"
     CDS             complement(4891..5610)
                     /locus_tag="ACHBB7_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9209963.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHBB7_002021"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHBB7_002021"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM9209964.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHBB7_002022"
     CDS             7161..8558
                     /locus_tag="ACHBB7_002022"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9209965.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHBB7_002023"
     CDS             8639..9079
                     /locus_tag="ACHBB7_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9209966.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHBB7_002024"
     CDS             9227..9553
                     /locus_tag="ACHBB7_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9209967.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHBB7_002025"
     CDS             9554..10303
                     /locus_tag="ACHBB7_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM9209968.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHBB7_002026"
     CDS             complement(10383..10838)
                     /locus_tag="ACHBB7_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9209969.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3557 a         1960 c         2303 g         3397 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagt
//
LOCUS       DAYYKN010000092        10955 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000092 DAYYKN010000000
VERSION     DAYYKN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.092"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            250..1485
                     /locus_tag="ACHBB7_002027"
     CDS             250..1485
                     /locus_tag="ACHBB7_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9209970.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2484..2744)
                     /locus_tag="ACHBB7_002028"
     CDS             complement(2484..2744)
                     /locus_tag="ACHBB7_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM9209971.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2771..4072)
                     /locus_tag="ACHBB7_002029"
     CDS             complement(2771..4072)
                     /locus_tag="ACHBB7_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9209972.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4364..4822)
                     /locus_tag="ACHBB7_002030"
     CDS             complement(4364..4822)
                     /locus_tag="ACHBB7_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9209973.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4835..6166)
                     /locus_tag="ACHBB7_002031"
     CDS             complement(4835..6166)
                     /locus_tag="ACHBB7_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM9209974.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6177..7619)
                     /locus_tag="ACHBB7_002032"
     CDS             complement(6177..7619)
                     /locus_tag="ACHBB7_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9209975.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(7606..8370)
                     /locus_tag="ACHBB7_002033"
     CDS             complement(7606..8370)
                     /locus_tag="ACHBB7_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM9209976.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8474..9349)
                     /locus_tag="ACHBB7_002034"
     CDS             complement(8474..9349)
                     /locus_tag="ACHBB7_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9209977.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(9604..9688)
                     /locus_tag="ACHBB7_002035"
     tRNA            complement(9604..9688)
                     /locus_tag="ACHBB7_002035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9652..9654),aa:Leu,seq:caa)
     gene            complement(9706..9777)
                     /locus_tag="ACHBB7_002036"
     tRNA            complement(9706..9777)
                     /locus_tag="ACHBB7_002036"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9743..9745),aa:Gln,seq:ttg)
     gene            complement(9787..9859)
                     /locus_tag="ACHBB7_002037"
     tRNA            complement(9787..9859)
                     /locus_tag="ACHBB7_002037"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9824..9826),aa:His,seq:gtg)
     gene            complement(9921..10005)
                     /locus_tag="ACHBB7_002038"
     tRNA            complement(9921..10005)
                     /locus_tag="ACHBB7_002038"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9969..9971),aa:Leu,seq:caa)
     gene            complement(10026..10096)
                     /locus_tag="ACHBB7_002039"
     tRNA            complement(10026..10096)
                     /locus_tag="ACHBB7_002039"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10062..10064),aa:Cys,seq:gca)
     gene            complement(10142..10213)
                     /locus_tag="ACHBB7_002040"
     tRNA            complement(10142..10213)
                     /locus_tag="ACHBB7_002040"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10179..10181),aa:Gln,seq:ttg)
     gene            complement(10225..10297)
                     /locus_tag="ACHBB7_002041"
     tRNA            complement(10225..10297)
                     /locus_tag="ACHBB7_002041"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10262..10264),aa:His,seq:gtg)
     gene            complement(10300..10373)
                     /locus_tag="ACHBB7_002042"
     tRNA            complement(10300..10373)
                     /locus_tag="ACHBB7_002042"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10339..10341),aa:Trp,seq:cca)
     gene            complement(10380..10460)
                     /locus_tag="ACHBB7_002043"
     tRNA            complement(10380..10460)
                     /locus_tag="ACHBB7_002043"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10424..10426),aa:Tyr,seq:gta)
     gene            complement(10462..10537)
                     /locus_tag="ACHBB7_002044"
     tRNA            complement(10462..10537)
                     /locus_tag="ACHBB7_002044"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10502..10504),aa:Phe,seq:gaa)
     gene            complement(10573..10645)
                     /locus_tag="ACHBB7_002045"
     tRNA            complement(10573..10645)
                     /locus_tag="ACHBB7_002045"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10610..10612),aa:Asp,seq:gtc)
     gene            complement(10655..10727)
                     /locus_tag="ACHBB7_002046"
     tRNA            complement(10655..10727)
                     /locus_tag="ACHBB7_002046"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10692..10694),aa:Val,seq:tac)
     gene            complement(10743..10814)
                     /locus_tag="ACHBB7_002047"
     tRNA            complement(10743..10814)
                     /locus_tag="ACHBB7_002047"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10779..10781),aa:Glu,seq:ttc)
     gene            complement(10829..10918)
                     /locus_tag="ACHBB7_002048"
     tRNA            complement(10829..10918)
                     /locus_tag="ACHBB7_002048"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10882..10884),aa:Ser,seq:gga)
BASE COUNT         3497 a         2352 c         1764 g         3342 t
ORIGIN      
        1 ctcatgttta tttttaatga cacaacttct cgtatttatc tggcacaaca tataaatcga
       61 tcaaaaaatt gttgcgtgtt tttttgaaaa ttggagtttc aacttgatta caaaatttaa
      121 atttagaagt tgtgaaaaca gcgagagaca gtttaatatt caaaatatac ataggctact
      181 cgaacgctgt gttttttagt atactagtaa gaaacgaagg gatagactca ttccaataag
      241 gaggatttca tgaagacaat tctttttgct cctgcagtat tcaatttagc tgagacaaca
      301 cgaatgattg aggttgccaa agctatgaaa gaccgttatt actgtgtatt ctttggcttt
      361 tctaaaactt ttgctccttt gatcacagaa agtggctttg agtatcattt actagcgccg
      421 atccttacga aaaaacaaga agaacaaatc ataaaatttg accagttgcg tacacttaaa
      481 aaccctttta ccaaagaaat ggttgcctct cgtatcacta gtgaacagag tctgattgct
      541 aagtttgagc ctcgactgat tgtgacaggt tcgaatgtga caattttttt atcggcccgc
      601 aataaaaaga ttcctttagt atatgttaaa ccctatgctt tgagtgaacc tttttttcaa
      661 tcagagcatg cgctgatgcc ccacgagtta cgtttcttcg gccctttaag caatcccctt
      721 tggcactgga caaaaaagcg tatagtaaat acccgatgga tccccaatgc cttcaaacac
      781 gctgcacaaa aagaaatgct tcctttacca aaaacaagtt tacaaatgat ggatgctgat
      841 ctaaatctca tcacaacgcc taaactattt ttggaagaag aacagctgcc ggaaaattat
      901 cgaatcgttg gtccaatttt tgctaagctc agtacttcct tgcctgaaga agtgctaact
      961 tttatacagc gaaagcaaca agaaagaaaa cgtattatct atttcgcaat gggtagttca
     1021 ggcaatccgc gtatgttacg caaaattttg ttcttcttac aaaaacaacc ggatctagcg
     1081 gttattgcac ctattgacta tctgcttgat gacattcaat ctgactcaga agatctttta
     1141 ataacagaat atctgtctag tcatcttttg catgaattca ttgatttttc ttttatccac
     1201 ggcggggaag ggaccgtcca aactgcctgt gaatccggca aaccgtttat cggtgtgggg
     1261 atgcaatttg agcaaagctg taatattgac tattgtgtaa aatacggaaa cgcaatcgcc
     1321 ctcacaaaac cagtgactga aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc
     1381 aagattcgtc agcaggcacg cacgctgcaa gccgtttttc caataaatgg tccacaacaa
     1441 gcaattcaag aaatcgaaac cttcctgagc aaccacccaa tatagtttca agtagaaaaa
     1501 gcttttttaa tagagactca tcagtaattt cgctattcga gacccagaca gtgaaaatac
     1561 aaaaaaagta aatagtggat tgaaaagtta gagttactta aattacggtg tctaaaaaac
     1621 acaaaatcat ccttaaaata aaagatctga cgctatccaa atgagcatag gtagcgtctg
     1681 ccatttattc ttagtcaaat tctttgacac ctttctctgg ttggatcatc tcaacgtttc
     1741 ttcaaaagaa agaccataat aattaggcaa atacattttc ttaaaatgtg tcatatagat
     1801 ctagaagcat cgctttgtag agatttgtgc cgctagtccc ctcaacaatt actaaatcaa
     1861 atgtaggatt taccacttta tctttgataa tcaacctccc tcttctgttg agacaatgat
     1921 ctcgcaatcc gatgcatact cctatataaa ctgccagcaa tccccacata ttgagtagat
     1981 catgtcagaa tgttctatgt cgtcagcaac gatacactaa aagtcacgtt caccatgact
     2041 gattgctatt cccagcacaa ttatttctat acacgtttca tgatttgaat aaacactcac
     2101 atcatacata ttaccattca tacaccatac tgcagcccct actatatggt tgtaagctcc
     2161 caaatctata tttgacttga tcactttggt agtagatcta ctaattctaa atccattgct
     2221 gataaaattt ccattttttc ctccttcttt ctgccaaaac agttaaaaca ataataatct
     2281 ttatgtaaaa aagggctgtg acaaaagcct tgtcacagtc ccatattccg aataaacggt
     2341 ggtcaaaagc ttgcatctgc ggtaataagt tcaaccaaac gaaaaataag ccaacatttc
     2401 acaaaaattt aagaagcaat tttcgtaaat ttcttcttat ttcttgaagc taaccacttc
     2461 tgttacaacc acttttagaa catttaaagc ttcgatgtga caaaccatct atgctgctcg
     2521 atcataaaat tgatagcaaa aattccacaa agtacatatg tcccccatga aaacaaaaca
     2581 ttcatatgcg gtacaatgat ctttggaaga atcacgatca aaaatgatgc cagtgcccaa
     2641 atccatcgat tgatttgaat atgatatgta gctagcatat tcaatataag gtgactagcc
     2701 gttaataaaa agataaaaac aaaaaataaa atcagccaat acattgtttc ccaccttcta
     2761 aaaaccaaac ttagacagtc tggacttcgt tttctccacg actttgttca tctgacagca
     2821 tctttttatc ataagctttt acaaatggat aatagataat aaatgctatc aatgtgttga
     2881 tcatgatcaa gataacaaca cgccagtcat ttcctgctga aagataaccg ccgattggag
     2941 ctggtaatgt ccatgttggc aaaacagtaa atctattgac catttctaat ttgactgcaa
     3001 aataactgat aatagtcaaa actaaaggaa ctaagttgaa cgggatgaac atgtaaggat
     3061 tcaacatcat aggagctcca aaaattatgg gctcattgat attgaagata ccaggaataa
     3121 tagaaaaacg accaacctct ttcaagaatt ttgatttaca gaagaatata aacaaaacag
     3181 ataccacgat cgttgctcct gcaccaccaa ttgttaccgt ccattgataa aactgttcag
     3241 gagcgatata gggtaattgg gtggcagctg taccatctgc caatgccttt gcattctcat
     3301 ccaacatgat cagccacatt ggacgaacaa cagcccctac aatactcatg ccatggatac
     3361 caaatgacca caacaaatgg atcaaaaaca ttggtagtaa aacaccaagc aaattattcc
     3421 ctaaaatatt ggttaaaggc tcgaataacg ataataaggc agcgtttaca tcaaagcccg
     3481 cgacgcttcg aatcagccaa gtaattgtta aaatgactgc acctggaatc aatgcctcaa
     3541 aactacgggc aacagcaggt ggcacttgtt ctggcatttt gatagtaata tttcgatttt
     3601 taaagaaacg atatacttcc actgcaaaaa tcatagataa gatccccgca aacatcccag
     3661 atgctcccaa agaactcata ggtaagaccc agccaagagg tgcctcttca ggagttaaaa
     3721 cgtttacagg aacttgtaac ataaagaacg ttcctaaaga taatatacca cctgtcacac
     3781 tatctaatcc atatgattta gctagatccg aacccataac aaaagttgca taaatagcca
     3841 tcaatcccat cgtcatccta aagggtaaaa caatcgttgc cgaataaggg gcaataaatt
     3901 cattccatcc aggaaacggt aaattgataa agaccataaa aaatgtccca atcaaaatca
     3961 atgacaatgt tgaaacaacc ccattacgaa ttgctaataa atgacgctgt gtaccaattt
     4021 ttgccattgg tgggacaagt ttctcttcga cccatttcat cagcttttcc actaaaacca
     4081 ctccttttca ataaaatatg aaccattcat tgcatgacaa cgcaaagaat gttcataact
     4141 aattatataa ttaagaaatt ttatttcaat atattctctt aaataatcaa atttattttt
     4201 aagaatcctg aaaataagtt ttctaaatca taggagcaac aaaaaaattc gataattaac
     4261 aaaaatcaaa atgattaaat ctagtgattg aaacatatca tttcaaaaaa attttgtaaa
     4321 ttcacccata aatatcaccc tatttaactt ttttcatcgt acactaaaat tcaaaacaac
     4381 tatctttaat ctttacttct tggttttgtt ttaataaatt tccttcaaaa atgcctggag
     4441 aaactacaat gatcaccgta tctttttgtg cttgctccaa cttcttcaaa tctataatag
     4501 ctaacagtgt cccttctttt acctcctggt tactttcaac ccgtatctga aacggacttc
     4561 cttcgagttc aactgtatct aaacccatat ggatcagtat ttttctgcca gattcacttt
     4621 cgatcgttat ggcatgtcgg gtaggaaata tattatcgat tattccttta actggcgcat
     4681 ggatatatcc atcatgattc gttatagcgt atccaactcc tatcattttt gaagaaaaga
     4741 cctcgtcttt tactgctgtt aatggaataa tttgaccact agctacagct tttagaatgt
     4801 ttcttgtttt aaagaaatgc actttttatc acctctactt taatgctggc caatattttt
     4861 tgtttacttt gatcatttca tctagtattt tttttgcgac tctggcattt ggtacggttt
     4921 gattcaatgt aaatgcttgt aaagcttttt gataagaacc ttcaaagtaa gcatctacta
     4981 gtaacttctc tactgcaact tgtgcctcca tcaatccttt atgaaaatca ctgattggtt
     5041 ttcttagtgt aattggctcc acaccagttg caccaacata tgccggtatt tcaacaactg
     5101 catctgaccg tagattcgga atcgcccctt gattaggaac gattagcata aagcgacgac
     5161 gatcatcatt caacaaggaa atagctatat cgacgatata ttgtccatgc tccccaaaat
     5221 taaagttcaa tacttcttcc acaataccgc ggcgtatttt atcagccatt tctttggtat
     5281 ttttctctcg tccatccatg accatattag cacgtgtata ctctttgtca gtatgctcaa
     5341 cgaccatatc aggatataag taatactcta agtaattatt cgggatatta cttggaaaat
     5401 ttttcgtgat gactgacatc atttgaaagg ttttttgcca agttttatct cctatattaa
     5461 aatcggcgac ttgcatttct tttgttatta gtttttcaat aatctctggc ataatttctc
     5521 gttgcaattc tttatcataa atcgatgtat accagccaaa gtggtttaaa ccataatacg
     5581 taacgatcca attcttacga tcataaccgt aattcactgc aatcgtttct tcaattgaaa
     5641 tagtcatatc acaggcatta atcattttta cattcggata tttacgacga acagcttcag
     5701 aaacgatcga ctctggattt gtataattca aaatccatgc tttgggggca tagtcttgta
     5761 tatgatcgac aatttccaat aaacctttga ttgagcgcat cccataagca aatcctccga
     5821 gtccacacgt ttcttgtcct actaatccat gtttaagtgg aatcttttcg tccttttctc
     5881 gcatctccaa tcctccaaca cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa
     5941 gctttggatc atccgttgca cgaatgcgaa tatttgaaaa accttttctt ttcagcataa
     6001 atttaacaat caagtacata tccatatttc tttcttcatc aatatcatat aaacgtattt
     6061 cagataatgg caaccgtttt tcattgttca aaatggcttg aacgattcct ggtgtatacg
     6121 tactcccgcc tccagctata gtgataatct gttttttcat catcatcttt tcctttctat
     6181 tcttctaata atttattgaa ttctgccata acataagtca cttctaagcc gatgataatt
     6241 tgataatttt tttcagttgg tttaatgatc ccgcttatcc ctaattgctt gattcgctgt
     6301 tcctcgacca tggtgggatc cactacctct acccgtaggc gtgtattaca actatagaca
     6361 tccaggatat tggcttttcc tccaaacgct tgtagcattt ttgttgccat atattcataa
     6421 tttttacttg ctaaccctaa ctctgtttct tctaaggtta cttcattgcc aaactctgcc
     6481 ttagcttcac gtcctaatgt ctgaatgttt ctcttattga tcaaataata aaaagtaacg
     6541 taatacaagg caaaaaagaa aattccgatt ggaagaatca aaatagcatt ggtagcgatt
     6601 cgaaaattga tgagataatc aacaatacat gctccgaatg taaaaccaat tcgtatattg
     6661 aataagtaac agacaatccc tgctaaaccg gcaaaaagtg catggatcac gtacaacata
     6721 ggagaaagaa acatgaaact aaattcaaca ggctcagtga tatttgcaat aaatgaagtt
     6781 cctgcaccgc ttcccatgat tcctttgacc atctctttat tttctttata agctgcttta
     6841 tagatagcta gacatatagc tggtatacca aacatcatta ctacaaaaaa aactgataaa
     6901 aacagtccag cctgaggatc tccgttgata aagcgtgtca tctctcctct gaaaacttcc
     6961 ttgctaggag atgtatagct tcctaaatcg taataaatat acgtattcaa cacatggtgc
     7021 agtccaaagg ggatcagcaa acgattcaaa aacataaata tccccacacc taatgctccc
     7081 atatcaacga gcagttttgc aaaattatca atcccaattt ggatgagcgg ccagataaag
     7141 cccaaaaaaa cagctgcgat tgtttgtaga atcatcacca tagtcaatgg gaatttttcg
     7201 cccgcaaaat aagaaaaaac tactggtaat ttttgttctt taaagcgatt atacgtccat
     7261 gccgctacta aaccggaaat aattccacca aaaatcccca tattcacttc tgggtccata
     7321 atttctaagc cttttttcaa ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct
     7381 ttgtccttat ttttgacaaa accgattgca actcctatag caaataagat atccaagttt
     7441 ccaaaaacgg catttccagc tgcaccaatt actgggatgt tcagcatatc gtcagctgat
     7501 aaacgcatga tcaatcccgc taatggcatc gctgcaactg gaagtagcat cgctcttcca
     7561 agcttctgca catttgattt aacattcatt tttattcctc ctctattaaa tatcctcata
     7621 tgttactaac tcaatcaaag gatcattttt cactttttga gcaaatgctg aatgaattaa
     7681 aaattcgact tggtgggtac gaggatacac aaaacttgaa ccatttaaca attcctgatc
     7741 caaatacgct gcgtgacaca tgacttcgat cgttttatct tctctcaata tcatgagtaa
     7801 acttgccaaa tattcttcca cactcgtatt gatttgtcgt tttttttcat cagtttctcc
     7861 tacgacatcg aaacaaggct caaaataatc gacatgacga acttcatctt ttcgttcaaa
     7921 gttgctccga accggcaaat catatttttt tgctaatgta acaactacct cctgattttc
     7981 atccaaagta tgagtatgat gatgactgtc taaatgagtt ggtatgatcc ccgcatgata
     8041 aattttttga atctgagcat tccattcctg atacagttga tctttgtcaa tgttaaatgg
     8101 atgctgataa taagataagg atctgaactc tccgctttcg ataagagtat cgacagtttt
     8161 gaggagtggt ttgccacacg tcaatgttaa atggactcct acacctagcc taggcatttc
     8221 ctttcttagt ttcacagcat gctcgaaacc cggcatattt gccatcaatg tagttgatgt
     8281 caaaattcct cgctgataag catccataat cccataattc acaccatggc tatagccaaa
     8341 atcatccgaa ttaaaaatca cttttcccat ttcattctcc ttcttttttc tactaaaata
     8401 ggcgcaacca aataatcata gtccatttac tatgtgtcat ttggttgcgc ctatagatag
     8461 ttacgccctt attttaatag attcttgcta gatgaattgc caaatagatg atttcatttt
     8521 cttgaatggc aagttgaaat tgctgttcga tgatatcttt aatctttagt gctgtacaat
     8581 acgctttgct atgtttaaat gtttcagtaa aaaaaccaat attttctact ggcgtttctt
     8641 cctgataaat caagcgttta accaggaatt tgatatgaac cattaagcga tgataagtca
     8701 gtgattcttt atcataaacg tttgaatagg atttttctag atgggacaaa atcgtggtcg
     8761 tcatttctac tatttttaag ctctttaaac tattaggctc tcctagctca gcattaatga
     8821 tatgcatagt gatgaatcct acttcatttg accctaactc ggtttgatat cgatgattga
     8881 ttcgctcgac cacttctact gctatatcat attcatttgg ataaaggaat ctcatttctg
     8941 gcaacataat tgctgtgaca tctctatctt ctctttttcg ttgaatagca aaataaatat
     9001 gatctgtcaa tgtaatatag atatttgaat ctaatggaga ggagatccta gctgttatca
     9061 gttcaatcac ttcttccgca aaatccataa attcgattgg aatctgcgag actatctcac
     9121 tgaatcgttt gatcttttct ttagaatcta atacaaataa tttgtcagca tcctcaacat
     9181 caaatggctt actcacttta gcagaaaagc caatcccttt ccccatcgca attttttcta
     9241 ttccatcgtc atcgatcaat acagcattat gattcagctt tttcatcaca tacaccgtta
     9301 attcacctcc tctatatgat aaaacaaaac aacttgcaga ctccttcata catatttttg
     9361 tctccaaaca atttttaaag acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg
     9421 cctaattgaa tagtagcacc ctacataatt tacaaaaata gtttaacaca taataaaagc
     9481 gttatcaata tcttttttac tcgttttatg ataatacttg tatcgaattg atttggaggt
     9541 aattacataa aaacaaaaaa aatatagcaa ctctctataa gaaagttgct atattgataa
     9601 ttataccggc ggccggggtc gaaccggcac gtcctcaacg gacactggat tttgagtcca
     9661 gcgcgtctgc caattccgcc acgccggcaa atttaaaaat aataactggg gtagctggat
     9721 tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac cgcttggcta taccccaaga
     9781 ataaagggcg aatgatggga atcgaaccca cgaatgccgg agccacaatc cggtgcgtta
     9841 accacttcgc cacattcgcc atcataaaag atgttattta atttctcaat aaaaaactaa
     9901 gatgaagaca tcttagtcaa taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc
     9961 actggatttt gagtccagcg cgtctgccaa ttccgccacg ccggcaagtt gatttattag
    10021 tactaaggcg gtaaccggat ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc
    10081 acttggctat accgccatta tactattatc aatctataaa ataaagaata aattatagaa
    10141 actggggtag ctggattcga accaacgcat gagggagtca aagtcccttg ccttaccgct
    10201 tggctatacc ccaagaataa aaagggcgaa tgatgggaat cgaacccacg aatgccggag
    10261 ccacaatccg gtgcgttaac cacttcgcca cattcgccat ggcaggggca gcaggaattg
    10321 aacccacact aacggttttg gagaccgctg ttctaccttt aaactatgcc cctataaaat
    10381 ggaggagagt ggattcgaac cactgaaccc taaggaacgg atttacagtc cgtcgcgttt
    10441 agccacttcg ctactcctcc atggtggcgc gggacagaat cgaactgccg acacacggag
    10501 cttcaatccg ttgctctacc aactgagcta ccgcgccata ttttaaaatt attaattgaa
    10561 taacaagaac gacggttccg acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc
    10621 atgttaaccc ctacaccacg gaaccagttc attatggagg ttaacgggat cgaaccgctg
    10681 accctctgct tgtaaggcag atgctctccc agctgagcta aacctccaac attcaattaa
    10741 aatgacccgt acgggattcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc
    10801 ttgaccaacg ggccaggtaa atttgctacg gagagtaagg gattcgaacc cttgagacag
    10861 tttggcacca tctacatgat ttccaatcat gctccttcgg cctctcggac aactctccag
    10921 agtgaaagaa gctcaatact ctttctcaaa atgag
//
LOCUS       DAYYKN010000093        10645 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000093 DAYYKN010000000
VERSION     DAYYKN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.093"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..646
                     /locus_tag="ACHBB7_002049"
     CDS             23..646
                     /locus_tag="ACHBB7_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209978.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /gene="trpX"
                     /locus_tag="ACHBB7_002050"
     CDS             1096..2145
                     /gene="trpX"
                     /locus_tag="ACHBB7_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9209979.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ACHBB7_002051"
     CDS             2142..3023
                     /locus_tag="ACHBB7_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9209980.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ACHBB7_002052"
     CDS             3026..3796
                     /locus_tag="ACHBB7_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9209981.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ACHBB7_002053"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ACHBB7_002053"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM9209982.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ACHBB7_002054"
     CDS             5107..6270
                     /locus_tag="ACHBB7_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9209983.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ACHBB7_002055"
     CDS             6387..6797
                     /locus_tag="ACHBB7_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9209984.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ACHBB7_002056"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ACHBB7_002056"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9209985.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ACHBB7_002057"
     CDS             9370..9654
                     /locus_tag="ACHBB7_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209986.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="ACHBB7_002058"
     CDS             9814..10050
                     /locus_tag="ACHBB7_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9209987.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="ACHBB7_002059"
     CDS             10047..10445
                     /locus_tag="ACHBB7_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM9209988.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3715 a         1773 c         2253 g         2904 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt tttta
//
LOCUS       DAYYKN010000094        10556 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000094 DAYYKN010000000
VERSION     DAYYKN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.094"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2602
                     /locus_tag="ACHBB7_002060"
     CDS             <1..2602
                     /locus_tag="ACHBB7_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM9209989.1"
                     /translation="RTFSTLNKKTVSHIFSTADSQAIINYLEKQKLSEGTVNITYNPI
                     KQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDSEKKKAENVMEQVERYLKEK
                     RIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLLHRRFGVEDEVDIDTSVYN
                     PSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQIVEENSMMSTSR
                     VKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGVTLLIFDECPLRKHTNNSN
                     GSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESLESNQEYYFDQFK
                     LNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWDEIEIEGGCLQ
                     TNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLGWKKENEAMIFQLQDS
                     FGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIASIVLGYLNVT
                     KANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAIIKLASNNQGG
                     VPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSVLTLTSSEERLEN
                     YFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLKLFEQGIAKI
                     EATFVEAQKKIWAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPLRLEHIQTVLINS
                     YEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIQKNGDIKV
                     NIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLPTYEILL
                     PSSLKSYFRL"
     gene            2784..3023
                     /locus_tag="ACHBB7_002061"
     CDS             2784..3023
                     /locus_tag="ACHBB7_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209990.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLAFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNGRVAKEWYLVHS"
     gene            3039..4412
                     /locus_tag="ACHBB7_002062"
     CDS             3039..4412
                     /locus_tag="ACHBB7_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9209991.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGGKADRPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLAYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEAHEAKVVRHIFQLYLTKKYGYKKLCQRLTQQKFFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKTIFLQAQEIRQSRCTAKKDTYPYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKHKVIQQKKQEAQWLKAFEEGNCSLEELKQQLQALQ
                     KPRSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDHVCRIEVTKEKTVCGIYFRQMTQ
                     NILEGNE"
     gene            4416..6053
                     /locus_tag="ACHBB7_002063"
     CDS             4416..6053
                     /locus_tag="ACHBB7_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9209992.1"
                     /translation="METTPTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMSYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPKPILVKGTHEPIISEEDY
                     QKVQERLVLEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQQGEPIQVKDVQRILTSLDRFLAQSEKKQVKALYRTFIEKITFDPNYKEILEITM
                     KFTPAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6216..7148
                     /locus_tag="ACHBB7_002064"
     CDS             6216..7148
                     /locus_tag="ACHBB7_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9209993.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLK
                     NGKSDLTHSIKKMNSLRVAKAIVAMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPH
                     EDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFMPLDLSTGRKIRFSEIQY
                     EGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNI
                     NYIDSGSDLLIRWQVITNFLMELKKYSNTQTKFS"
     gene            7264..7584
                     /locus_tag="ACHBB7_002065"
                     /pseudo
     CDS             7264..7584
                     /locus_tag="ACHBB7_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            7762..8121
                     /locus_tag="ACHBB7_002066"
                     /pseudo
     CDS             7762..8121
                     /locus_tag="ACHBB7_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            8185..>10556
                     /locus_tag="ACHBB7_002067"
     CDS             8185..>10556
                     /locus_tag="ACHBB7_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9209994.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTK"
BASE COUNT         3764 a         1605 c         2044 g         3143 t
ORIGIN      
        1 gagaactttt tctacattaa acaagaaaac cgtttctcat attttttcta ctgcagattc
       61 tcaagcaatt attaactatt tagaaaaaca gaaattgtct gaaggtacag taaacataac
      121 ttataatcct attaaacaag aggtgattac acaaaaaaag cattctgtga gagacaatga
      181 tattgaaaaa attcggtttg tatttattga tattgaccct aaaaggaaag agggaagtgc
      241 aacagatagc gaaaagaaaa aggcagaaaa tgttatggaa caagtagaac gttatttgaa
      301 agaaaagaga atagaatcat ttgtaaaagt tgatagtggg aatggctacc atatttttct
      361 tcccataaat gaacaaccaa ataatcacga gacgattctt actattcaga attttcttca
      421 attacttcac cgaagatttg gagtagaaga tgaagttgat attgatactt ctgtttataa
      481 tccttcaaga ttatgcaaat tagttggtac accagcggtc aaaggaaagt ccacaaaaga
      541 gcgtccgcat cgtttatcta gtttcctttc tattcccgat aacatagata gaagtaattg
      601 ttttaaaatt cttgaacaga tagttgaaga aaattccatg atgtcaacct ctagagtaaa
      661 aagccatcca tcttttagag aagtgttacc ttttattcaa gcagactata aacaatggtt
      721 agctcactat cctgaattaa cttataccat aaaagagggc gatcataaag gagttacctt
      781 acttatattt gacgaatgtc cattaagaaa acacacgaat aattctaatg gttcttgttt
      841 gtctgttacg gctgataata agattcgatt tcattgtctg catgcttcgg ataaaaataa
      901 agatattcaa gactttgtaa aaaaatatcc gttgccaaaa cctgtaaatc cttctgtaga
      961 atcattagaa tccaatcaag aatattattt tgaccagttt aaactgaatc aagaaggagt
     1021 atttttggtg aaagaaggtc aagaagaaaa agttgcttgt ccgatgttta ttaagcaaat
     1081 acgcaaaaat atcgaacaaa aagtcattac acttgttctt gcctattatg tcgatcattg
     1141 ttgggatgaa atagaaatag aaggtggatg tttacaaact aataatttta aacaattagc
     1201 aaaacatggc ttaatcatta aagctagaag agaagctgaa gtcgttgact ttttacagat
     1261 tcaacgggag aatgctccaa tcttttttga acatgaatct cttggctgga aaaaagaaaa
     1321 tgaagcaatg atttttcagt tacaagattc ttttggacgt gaaattcctt ctgtattaac
     1381 taaaaactca tgctaccaat taacaactaa tggtacagaa gcggaatttg acgcattggt
     1441 tcataagtat gttttaggaa cgaacttaga acttgcttgg tcgattggta ttgcttctat
     1501 tgtgttaggc tatttaaacg ttacgaaggc taatactttt ttgagtttac taattaacct
     1561 acagggcaag agcacaacgg gtaaaaccac tacgttacac tttattgcct ctttatttgg
     1621 aaatccaact acggttctta gaaatatgaa tgctaccaat aaagccatta taaagctagc
     1681 atcaaacaat cagggaggtg ttccactaat actagatgag ttaggagcag caactcaaaa
     1741 taactttagc tcgttgatct atcaattagc ttcaggagaa gaaaggctaa gattagattc
     1801 taattgtcag cttatgccac aagagacttt ttctgttttg acccttacct cttctgaaga
     1861 gcgtttggaa aattatttca atgagaaatt aactggtttg aaagttcgat atatcgaatt
     1921 ttctgatatc aagtttacaa aggatgcgaa tcacgcagag aaagttaaaa gtctttcttc
     1981 taatactttt ggttggggta taaagttatt tcttttgaaa ctctttgaac aagggattgc
     2041 aaaaattgaa gcaacttttg tcgaagctca aaaaaaaata tgggctgttt tacctgaaca
     2101 ttctttgaag caacgcattg gtaataatct agctttgatt tatagtggag ctctcctaat
     2161 tcaagatata ctgggctatc ctttacgatt agagcatata cagacggtgc ttattaattc
     2221 gtacgaacag ttaattgtag atactagttt aggaagtgag caaacggtat ttgaccgagt
     2281 gaaaacgttg ttggtgatga atagccataa atttttaaca aacagtgaca acaaacatac
     2341 gcccagtacg atttggggaa aagtatcaat tcaaaaaaat ggggatatta aggtaaatat
     2401 ttttcctatc ccgtttgagg acttgttaag gcgagaattt caagtaaagg atctaaccta
     2461 tctttttaga caattgcttg attcgggtaa tatgtgtgca gaaaaaggga gaagaacgaa
     2521 acgtattcac atgaatcgtc aagttcttcc aacatacgag attctattac cttcttcact
     2581 caaatcgtat tttcgattgt aatttagaat aaaccaaagt atgaaagtga ggcaccatgt
     2641 tatggaaata tttcttattg atttagcaaa acaactgatt acttatctgt atgatgacga
     2701 gcaaacccaa acggaagtaa atgaagcttg atctatgctt catttacttc attcaatcaa
     2761 acatttaaga aaggggaatt ctaatgacta aaaataaaat gacactaaaa gcagaggttc
     2821 ttctgtatat tcaagaacat ttttctaatc aagccttttt tacaaaacca atttacttgg
     2881 ctttcgaaat aagaggggta tctgctggat ctattggagg tactcttcaa gcgttgaaga
     2941 acgaaggata tttagaaaat cactttgtgc aacgttcgtt caatggaaga gtcgcgaaag
     3001 aatggtattt agttcattca tagagaggtg attcaagcat gcgtgtggct tgttatgatc
     3061 gaacgtctgt ttatgaagaa gcgaaaatca gtaatgacgc tcaatatgat gcagtgaaag
     3121 agtatgtttt agagaaatat gaacaaattc ctcaacgatt ttcagacaaa ggagaatcag
     3181 gtggaaaagc ggatcgtcca gcttttcgac agcttagagc ttgggtttgt gcgtcagaag
     3241 aagagggggt gatctatgtc tggcgttatg atcgattggc acgtcaagcc aaaatagcct
     3301 tagaatttat tgagttgtgc caacaacatc aggttcaagt ttattcgatt tctgaacctc
     3361 ttcctgcttc tttctcgcac gcgtcaacga ataaactatt tgtgcaatta ttgttcactt
     3421 ttgcagaaca acaacgaaac gtcattattg aaaatgttcg tgccggactt gcctataaaa
     3481 agcaacaacg gcaatatttg tcttctgcgg ttccatatgg gtatcgttta gtgaatggga
     3541 aaatccaaca agaagcacat gaagcaaaag ttgtccgcca tatctttcaa ctttatttga
     3601 ctaaaaaata tggctacaaa aagctttgtc aacgattgac gcaacaaaag ttcttcttcc
     3661 gtgaacgtcc ttttcagccc tatcatattt atagtatcct aaaaaatcca ttgtactatg
     3721 gcgaaatcaa aggaggatct ttggggaagt atctaggaac ctttgaaccg attctctcga
     3781 aaacaatctt tttacaagcc caagagattc gccaatcaag gtgtacagct aaaaaagata
     3841 cgtatccgta cttattacgt caaaaaattc ggtgtccctt ctgtggtcgt catctgagtt
     3901 cgaagtatca atggaataca aagaagacga agacgcttca ttattatcat tgtaccgacc
     3961 gaagctgcca aggaatcttt cttcgggcag aaagggttga gtcagaagta ttggaaaaga
     4021 tacaggcttt ttttcaatac gaaggcgtct atcaagcctt gattgcccaa ttgacgcaac
     4081 aattagccga aaagcgaaaa gatgaaaaac acaaagtaat ccaacaaaag aaacaagaag
     4141 cacaatggct caaggcattt gaagagggca attgttcact ggaagaactc aagcaacagc
     4201 tgcaagctct tcaaaagccg aggtctactt cttcgtttct ttcactccaa cagtgtgaag
     4261 caaaagtgaa agaatggctt caactaaaag acctctctag tcgacaattg ttatgtgacc
     4321 atgtttgtcg gatcgaggta acaaaggaaa aaacagtctg tgggatttac ttccgacaaa
     4381 tgacacaaaa tattttagaa gggaacgaat gatctatgga aacgacacca accaaacgag
     4441 tcgcattgta tattcgggtg agtacccaag aacaagcttt agaaggacat agtattcaag
     4501 cacaagaaca aaatggacga cagtttgccg aacggatgag ctatgaagtg gtagaagttt
     4561 atgccgatga agggattagt gggaaatcca caaaaaatcg gttcgcctat caacgaatga
     4621 tgaacgatgc tcggaaccat cgttttgatc tactagtgat ttggaaattg acccgtctcg
     4681 gacgcaatat gttagatgtc ttacaaaccg tcgaggaact gttgcgatat gatgtggggt
     4741 tacattcgat ctcggaacaa tttgacgtga ctacttcgac aggaaaacta atgttacaac
     4801 tccttgggag tttcggtgaa tttgaacgca accaaattgc ggagaatgtt cagatgtcaa
     4861 tgcaaagctt agtgaaagat aaaaagcgtt atgcaggtgg acgacgttta ggctatgtca
     4921 gtggtattga cacgaatggt caaaaacaat tggtcattga accagaagag gcgaaaattg
     4981 ttcgtctgat ctatgcaaaa tatttaggcg gagcagggta tcgagcgatt gccaattttt
     5041 tgaatcgtca aggatatcaa acagtcaaaa agaatgcttt ttctaccacg gcagtcaaag
     5101 atatcttacg caataaaatt tatgcagggt atttagaata tgcacgctat atcaattggg
     5161 aaaccaaacg acgaaagggc aagaacccaa aaccgatttt agtgaaaggt acccatgaac
     5221 cgattatttc ggaagaagat tatcaaaaag tgcaagaacg ccttgtttta gaaggcaaac
     5281 agcccaaatg gaaccataca ggtgaaaatg tattgacagg cttgttacgt tgtccagaat
     5341 gtggggcacc gatggcagcg agtaatgtca caaatactct aaaggatggc acaaagaaac
     5401 gaattcggta ttattcttgt tcagtctttc ggaataaagg agcaagtgtg tgtcatgcca
     5461 attcgattcg tgccgaacaa gcggaaacat ttgtcgccga ccgattaaag gagatcgttc
     5521 agctaccaga agttcttccc cgattggtgg cagcgttaaa tgaagaaata gtgcgacaaa
     5581 gccaaccatt ggaacaagaa ctcgtggtgt tactggaacg aaaggaagaa ttgaagacta
     5641 aaattgaaaa atgggaagct gctttggaag atagtcctga attgtttccg atgttaaaag
     5701 accgcttaga cgaattgact gaaaaacgac gacagttgca cattcgagaa aatgaaatct
     5761 tagggatctt tcaacaacaa ggagagccga ttcaagtcaa agatgtccaa cggatattga
     5821 ctagtttgga tcgttttctt gcccagagtg agaagaagca agtcaaagcc ttgtatcgta
     5881 cctttattga gaaaataacc tttgatccta actataagga gatattggag attacaatga
     5941 aattcacgcc tgccgtggta caacaattga atgagcaata tcaaatagcc gtatccaaaa
     6001 caaaggatac ggctattttt gtgttaaaga caccctttac gttaacgatc tagtctaggt
     6061 tatatcaaac aacgttagag gggcgctaac gcttctctag cgatttgaaa tcgttagatg
     6121 agtcgttagg gaggtcctta tcataaatag ggatatttat taatgaatat ttgaaatata
     6181 attgtatata agaattaaaa agagtgggga tagatatgaa aaagatgggt aagtgtattt
     6241 tatgtgaaaa atacactgaa cttactaaag aacatgtacc acctaaaaaa tcagggaata
     6301 ctggaggtaa aaagaccaga actggtaatt tagatgattt ccttaagagc gattttacaa
     6361 aaggtgattt tccgaagggg attaaaagga aacctcaagg aaatgtttat tatacgttat
     6421 gtagtaaatg taatagtttt tttggtagtg agtatgttga ggaatatatt agatttgcag
     6481 aagacaataa aaatttcctt tataacaata caagtttaaa gaatggtaag tctgatttaa
     6541 cacattcaat aaagaaaatg aattcactta gagttgcaaa agcaattgta gcaatgtttt
     6601 ttagtttaaa tggggatgaa gattcaatgg ataagccatt cttagattcg gtacgagaat
     6661 atcttagcaa tcctaaaagt actttatttc ctcatgaaga ctacaaaatt ataatgaatt
     6721 attatcttcc aataggtgct ccacagaatt ctaaaatcaa aattcctaat gacattgtaa
     6781 atgatggtga cactcacaat gtaataaaag gttttatgcc ccttgatcta tcaactggga
     6841 gaaaaattcg attttcagaa attcagtatg aaggaattgg ttttactctt atagatttaa
     6901 aaaattcaac ttttgaaata aaagaaggat tagatttaaa aaatctcctt tgtgcagatg
     6961 ataaaatcaa aaaaatacaa ttgtacaaag tccctatatt aacaccaaca gtacttacaa
     7021 tgttggagat ggaaaataat attaattata ttgactcagg ttcagatttg ctgattaggt
     7081 ggcaggtaat aactaatttt cttatggaat taaaaaaata cagtaatact caaacgaagt
     7141 tctcttgaag ttataaagaa tacaataaat ataaatttcc tttagactat attaaaatca
     7201 gcaatcgaag gaaaataaac gaagaaaatg gtacgcaaaa tctaactatt agagcaaaaa
     7261 caggaagctg ctttggaaga tagtcccgaa ttgtttccga tgttaaaaga tcgtttaaat
     7321 gaattgactg aaaaacgaag acagttacaa attcgagaga atgaaatctt agggatcttt
     7381 caacaacaag gggaaccgat tcaagtcaaa gatgtccaac agatattgac tagtttggat
     7441 tgctttctta tccagagtga gaagaagcaa gttaaagctt tgtatcacac ctttattgag
     7501 aaaatcacct ttgatcctaa tcagaagtag atattgccga tcccgatgag attcacacct
     7561 actgtgattt gataattgaa tgagtaatat caaatagccg tatccaaacc aaaggatatg
     7621 gctatttttg tgttagggat atacgtcacg tagcttgtta tcaagaataa gtttctttat
     7681 ttttttgctt tttctaaaac tgagtatgct aaaataagag ggtatatagc aactttaaat
     7741 aagagatagg agtgtatctt tttgggtgca gaattaaatc agaagttatt tagtgcggca
     7801 gataatctcc gtagtaaaat ggatgcgtca gaatataaaa attatttatt ggggctgatt
     7861 ttctataaat atttatccga ccgtttgttg gaacaagtgg ttcttttagc agatgaatcc
     7921 ttagaggaat atgacacagt aagcaaacaa acgatgttgt atcgtgaatt actttcagat
     7981 gaagaatcaa aagaagatct gattgcaaca atcgttgata ttttggggta tgcgatttcg
     8041 ccagagtatc ttttcaacgt cttagcggat caagcaaaac aagcaacgtt ccaattgaat
     8101 gatctaaaca aagccttttt agcaagtcat atttatggaa aaatactgtc ggcaaaattc
     8161 gaatagtgtc gaggaggtgc tgtgatggaa gaattaaaag aacgcattgt caattatgtc
     8221 atagagaact ctgagattaa aaataaatgg gggaacaata cagcacagaa aatatctgaa
     8281 aaattaaatg ttagtcgtaa tattgtctct caatatttga atgaattcgt aaaagatgga
     8341 gaatttggaa aaatcaatac tcgtccagta atcttctttt ctttaccgga gaaagttcca
     8401 aatcaacaaa tttttagctc ttcagaggaa gcggaaaaat ggtatgatag ctcttttagg
     8461 gttggaatag aaagggttat cggaagtcaa ggaagtctgc gaaatgtaat tgctcaaatt
     8521 aaagcagcaa tctactatcc gccaaatggc ttacccattt tacttcatgg cgagactgga
     8581 actggaaaaa gctatttagc aaattatgtg tataaggatt tgttggccaa taattatata
     8641 gaagagaagg catctttcat ttcagttaat tgtagtgaat atgccaataa tccagaactt
     8701 ttcttgtcca atttttttgg gcatgtaaaa ggtgcatata caggagccga agatggaaga
     8761 aagggtttag cagaattagc taataatgga gtcttatttt tagatgaaat tcatgcattg
     8821 agcaatgaat gtcaggagaa actttttcag tttatggata cgaggaaatt tcatcggatg
     8881 ggtgataatg aaaactggta cacatcaagt tgctggatta tatttgcaac atcagaacca
     8941 cctgaggata aactattatc aactttgttg agaagaatac ctgtaaagct aacacttcca
     9001 acattagaca gtcgttctct ttatgaaaga aaacagttaa tcaagatatt ttttaaatca
     9061 gaagaagaaa gactagcaaa gaaaatactg atatctcaac aattattaga ttttttatta
     9121 aactatcgct ttagtgaaaa tgttggcaat ttgaaaaata tcattcaatt gacctgtgcc
     9181 aatgcctttt atttcttaga tgatttggaa gaactttata ttcagatgta tcatttacca
     9241 caatgggtga atttcaacgg catgaaggtt atgaaaagac agaatgaatt aaaatatatt
     9301 caaatggatg atttggaaaa agtagacgaa gtttttaata tcaacgaact tatagagtat
     9361 ctttatcaaa cgtggaagaa agacactgat aagcagcagc tttttgagaa atatgataga
     9421 tattttagac aaaagaatgt tgatatccaa aataattcag ctttaacata tcccacctat
     9481 caagagattc gatttagaca gtatgttaag gaaatagaga gagattttca aatagaattt
     9541 gactattatt atcttcaaat aacgtctatc tttttgaatt cttggattta tcaaatccaa
     9601 ttaacatcgg acgataaatt atattccttc atcaatgata ttaatagttg gtgtagtcga
     9661 gaagttcaat tagtcaatcg ttttattcaa ataggggata tttatttaac aaaaattcaa
     9721 aaaaatgttt tggttatttt atttaagtta atgtgtagta aagaatttca gcaaaaacgt
     9781 ttagcactga ttttggctca tggagatcta acggcaacct gtatagcaag tactgctaat
     9841 aggatcgttg agagatatat atacgacgcc attgatatgc cactaatgac ttcaagtagt
     9901 gagattgcag acaaaattaa tagatactta caagagtgta ccggatatga agaaattatt
     9961 ttacttgttg atatgggttc actggagaaa atttatctaa atttggaact agacgctcaa
    10021 aaaaaattcg gactactaaa caatgtttca acaagaatgg ctattgatgt tgcaaatcag
    10081 atattattag atttaccgct taaacagatt cttgaacaag taaaagaaaa tcaaaatgtg
    10141 gattatattt atgaatcatc aaatattaag aaggatatga ttgtctgttc ttgtgcaagc
    10201 ggattaggaa cttcgtttaa attaaaaaag atacttgaag atagctttcc aagggatact
    10261 gatcttgtca tagaaacgtg tgactattac tctttagtga atgaaaacta tttatcggag
    10321 cttcgtaaag agaacaatat tctttttatt gttggaacac taaatccaca tctagaagat
    10381 gtagaattct tttcaattga agatatgatt ttaggaatga atataagtaa attctccaat
    10441 caattgaata gacactttaa tgataatgaa ctgaaagttt tgaacaataa tattctgaag
    10501 aattttagtc taacgaattt aatggagcaa ttgaccatat taaatccgac taaatt
//
LOCUS       DAYYKN010000095        10318 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000095 DAYYKN010000000
VERSION     DAYYKN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.095"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..904)
                     /locus_tag="ACHBB7_002068"
     CDS             complement(71..904)
                     /locus_tag="ACHBB7_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM9209995.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1208..3373)
                     /locus_tag="ACHBB7_002069"
     CDS             complement(1208..3373)
                     /locus_tag="ACHBB7_002069"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM9209996.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3633..5927
                     /locus_tag="ACHBB7_002070"
     CDS             3633..5927
                     /locus_tag="ACHBB7_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM9209997.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5920..6585
                     /gene="pgmB"
                     /locus_tag="ACHBB7_002071"
     CDS             5920..6585
                     /gene="pgmB"
                     /locus_tag="ACHBB7_002071"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM9209998.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6578..7585
                     /locus_tag="ACHBB7_002072"
     CDS             6578..7585
                     /locus_tag="ACHBB7_002072"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9209999.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7607..8626
                     /locus_tag="ACHBB7_002073"
     CDS             7607..8626
                     /locus_tag="ACHBB7_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9210000.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="ACHBB7_002074"
     CDS             complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="ACHBB7_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM9210001.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT         3198 a         2242 c         1909 g         2969 t
ORIGIN      
        1 ctctccaaac ggtataatcg tttggagagt tttattttaa tcagtttttt tatgttatat
       61 gttatatttt ttattcgttc acatctaatt cgatttccac cccgtagtga tcactgatga
      121 ttggactatc gatcccatca aaaatccgtt gatatttttt aacctgcatt ccttcaggga
      181 cgaaaataaa atctatacgc aatttttctg tattttgttc ccaaccatct attttctttt
      241 ccacagtagc tgcttttcct ttttcattcg cgacaacata cgaatcttga agaagaaggc
      301 aactttctct tacagtataa taaccttgag agtctactgg attattgaaa tcacctagga
      361 aaaaaagtgg tacgcgagtt tccaaaaagt agttttccaa gtttttccac tcaaaataaa
      421 agccactttt tttatctgtc caccaagaaa agtggcagct cgagacaaaa atgtcctgat
      481 catctatctt tgttttccca atcaagatct tgcgtgtctg ataatcttct ttcttttgaa
      541 attgggagac ggtaaggacc tgactttcca atgttttttt cgtaagcagt gcatttcctt
      601 cttcatattt atcataaccg atgtggctca tttcccaact ccaaaaatac tcttttccgt
      661 gttcctttaa aagctgaact aaacggtagg caaaattatc tttacggata ggtacttgat
      721 tctttaccgg acagaacttc attagcttcg caggttctac ttcttcagat tccagtaatt
      781 ggttgatttc ttgtaaagca atcacttcat aatcttctaa caggatctta tctgctaatt
      841 cttgcaattt tttctctggt tctggctcaa gccagctatg cgtgttgagt gtcatgaatt
      901 tcattggttt tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa atttttgtca
      961 caacctctcc agcaaatagt gttctcctgc taactcctca gtaataagct caatggacca
     1021 ggagctattt tttgtttggc tgtttttatc acttggtgca gggcagcttt ttcacgacct
     1081 ctccactaaa cagtattcaa actgctgctt ctcggtaata agcccaacca aacgaaaaat
     1141 aggaggagct atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa
     1201 cctctcctta cattttaatg gagccgatca ctttaccagc tgtcactgta cctaattgct
     1261 tgatcactac ttcttctact ttatcgtttg taaatacgat catgatcgtt ggatcttttt
     1321 cttcgttttg gatcacatca agatccatct cagctaattt cgttcctgtt cctacttctt
     1381 ggccttcttt gacagcaatc tcaaaagctg gctgattcat ttggactgta tcgattccca
     1441 tatggatcaa aacttcgata ccatctttcg tttccagccc aattgcatgt ttgctaggga
     1501 aaatacttgt cacttttcca gcgatcggcg caacaacttc tcttgatgtt ggaataacag
     1561 caaagccgtc acccatcatt ttttgtgaga ataccggatc ttttacttca gataaaggaa
     1621 tcacttctcc agaagctgcg gaaactagtt cttgttctat acctaagtat tgcttactac
     1681 ctgcttgtcc atttcctgta gaaaccattt ctggctctgg aatctctgct cctgattgca
     1741 gcagatcttc gatatctgat ttcaagacat ctgcttttgg tccataaact gcttggactc
     1801 cattgtcttt gacgatcaat cccatcgccc ctgctcgttt ccaggcttct tcagtcccta
     1861 ctttttctcg atcatttaca cttacgcgta accgagtcat acaagcatcg acatctttga
     1921 tattttcttt tcctcccagc aaatgaatga tctggaccac ttgctgatct gctgttcctg
     1981 ttccgctgtt agatacaacg gtttcttcac tgtcattgtc gtaatttccg ttacgtcccg
     2041 gtgtagcaaa gttgaatttt ttgatcaaga aatttgctaa gaaatacgta acaacaccaa
     2101 aagcgatcac acacaacacg aagttgataa gatctccacc tagacccgct ttgatagcta
     2161 acggtgtccg agtcagcaat tcgatattcc caaatgaatg gacccgcaat ggcaagatat
     2221 ctgccatcgc aaatgcagcc ccttggatca ctgaataaat gacatataat ggcacagcag
     2281 caaacataaa catgaactcc aatggttctg ttacacctgt tagaaagaca gccaacgctg
     2341 ctgaaaaata cattgattta tatcttgctt tcttatcagg atcgacatta cgatacatcg
     2401 caaaagccat tcccatcaag atacctgaag aaccaatcat ttgtcctact ttgaaacgag
     2461 ccggcgtcca gttatgcaac acaaactcat attgcgacat atcccctgca cctttaagat
     2521 tcactaaatc agtggcccaa gccaaccata acggatcttg cccaaatact tgtgtccccg
     2581 cctgtgcgcc tgataaaatc tcatacgatc cgccaagctg tgtatagttg ataggaatcg
     2641 tcagcatatg atgcaatcca aatggcaata gtaaacgttc taatgttcca aataaaaacg
     2701 gtgctagaat cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa ttattgattc
     2761 cagcctgtac gtttggccac accacagcaa gaatcaatgc cacgattgtt gaccataaga
     2821 taacaacaaa cggaacaaag cgctttccat tgaaaaatga caaagcatcc ggcaattttc
     2881 tataattata gtacttatta taagccattg ccccgacaaa tccagcgatg atccctacga
     2941 atactcccat attcaaagct ggtgcttcca atacacttgt aaaaaatcct ttaaccatga
     3001 tcttcgtacc gaacagagtg tgggtaaatg catcttcatt cgcaagcatg tcacttgtga
     3061 caccaaagat agccccagtg atccgattga ttaaaataaa tgagatccca gctgcaaatg
     3121 ctccgcctgc tctttctttt gcccaacttc ctcctattgc taaagcaaat aaaatatgaa
     3181 gattaccaat aattgcccaa ccaatatttt caattactcc gcccgttgtg acaagcggcg
     3241 tccaatcagg attgatcaag ggaattgatt tccctatact gatcatcaac ccagctgccg
     3301 gcataactgc taccacaacc attaaagcct ttccaaattt ttgccaaaac tcaaaactaa
     3361 atatcttttt cattccgttt agctccttta cgcaatcggt ttcgttaaac gtattatagc
     3421 gtgcgttttc ttttttgtaa atgctttatt ttttattttt ctgtctatac cattctataa
     3481 aacctataat acgtgtgttc ccttctataa ttttctccta aaaatttatt tttttaagct
     3541 tttttctatg aaatattttc ttttgtctat tgacttgtgc aatcggttgc gtttattatg
     3601 aacgtataaa acagctcatt aggaggagaa aaatgaaaca gatcaaacga ctatttgcta
     3661 ttgatccatg gaaaatcagc acgacacaat tagataaaaa aaatcttcga ctgcaagaat
     3721 ctttaacaag tattggaaac ggttatatgg ggatgcgcgg gaactttgaa gaaacttact
     3781 caggggacca ccatcaaggt acttatttgg cgggtgtctg gtatcccgat aagacacgtg
     3841 tcggctggtg gaaaaatggt tatccagaat actttggaaa agtgatcaac gccttgaact
     3901 ttatcgcgat agatatttcg atcaacgatc agctgatcga cttggcttct ttggaaccag
     3961 aagactttta ctgggaatta gatatgaaaa atggtgtatt atctcgtaca ttcacaatca
     4021 ctacttctac taataaagta cgcatttcct tcgaacgttt tctgagcatt gtcaaaaaag
     4081 aatcagctta tatctattta actatagaaa tgcttgaagg tagcggaaca gtcaaactcg
     4141 tatcaaaatt ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa catttttgga
     4201 atgagattga tcgtggaact atagaagaaa tcggctattt gacgactaaa actattccaa
     4261 acaactttgg gattgaacaa tttactgtta ctgcagcaat gcgccatttc gttaatggaa
     4321 aatcagtcat ccctgactat cacgaagata ctttagcatt acatggttgc ttgtctgcag
     4381 aattatctca aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt actagtagag
     4441 atattccaga agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac cagacaaaca
     4501 attatatcca agcaaaagaa gagcaaacag cggcttgggc agaacgatgg aagcttgccg
     4561 atgtggtcat cgaaggagac gaagaagcac aacaaggtat tcgattcaat ttgttccaac
     4621 tattctctac ttactacggc gaagatgaac gattgaatat cggccccaaa ggattcacag
     4681 gagaaaaata cggcggagcc acttactggg atacagaagc gtatgctgtc cctctttatc
     4741 ttgctttagc caaacctgaa gtaactaaga atctgctgaa atatcggcat aatcaactgc
     4801 cgcaagcaat ccacaacgct cagcaacaag gattaaaagg cgcgctttat cctatggtca
     4861 ccttcactgg tgtcgaatgc cataacgagt gggaaatcac attcgaggag attcaccgaa
     4921 atggcgcgat tgcctatgca atctacaatt atacgaatta caccggcgat acggaatatt
     4981 tgaaaaatga aggactggaa gttttagcag aaatcgcacg cttctgggca gaccgtgtcc
     5041 atttttcaaa acgagcaaat aaatacatga tccacggtgt gaccggccca aacgaatatg
     5101 agaacaatat caataacaat tggtatacaa atacgatcgc agcttgggtc ttgcgttaca
     5161 cgcgagaaaa ttatttaact tatcaaaaag aagctgctat cactatcagc gaagaagaac
     5221 tagccaaatg gaaagatatc gcagagaaca tgtacttccc agttgacgaa gaattaggaa
     5281 tctttgtcca gcatgacaca ttcttagata aagatctcat gcctgtatcc gatattccca
     5341 caagcgatct tcctctgaat cagcattggt cttgggacaa aatccttaga tcttgcttca
     5401 tcaagcaggc agatgtacta caggggattt atttcttcaa tgatactttt acaaaagaag
     5461 aaaagcaaaa aaactttgat ttctatgaac caatgactgt ccatgagtct tccctttcgc
     5521 caagtatcca ttcgatttta gcagcagaat taggaatgga aagcaaagct gtagaaatgt
     5581 accagcgaac agctcgcttg gatttggata attacaataa cgacacagaa gacggcttgc
     5641 acatcacttc catgactggt agctggttgt cgattgtcca aggatttgct caaatgaaaa
     5701 ctgcccatga tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa gcttattctt
     5761 tccatatcaa ttatcgcgga cgtttattgt tcatctcagt cacagatgaa caagttactt
     5821 tcgagttatt agaaggttct cctctagaaa tttacgtcta tgaacagtct tattctttag
     5881 cggatcgtct agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg ggtattgttt
     5941 gatttagacg gtgtcattac cgatactgct gaattccatt atcgtgcttg gcgaaagctt
     6001 ggtcaggaaa tcggcattac gatcgaccac acattcaacg aacagctaaa aggcgtcagc
     6061 cgagaagact cactctcttt gcttttagct tatggcggaa aagaacactc tttttcaaag
     6121 gaagaatttg cagaattagc gaaaagaaaa aacgattatt atttagagat gatccagacg
     6181 atcgaaccaa aagatgtctt ccccggggtc gtgcctttac tggattcttt aaaagaagaa
     6241 aaaatcaaga ttgctcttgc ttctgcaagt aaaaacgggc cattcctact tgaaaaaatg
     6301 ggtcttacac cttactttga tgctattgcc gacccggcag aagcaacaaa cggaaaacct
     6361 gcgccagata tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga agaatgtatc
     6421 gggatcgaag acgcgcaagc cggtatccaa gccatcctct caagtggcgc tcaaccaatt
     6481 ggcgtgggac gtgctgaaga ccttggagag gagatcccgc ttgtccctga tacgactttt
     6541 cttacgatcg attacttgaa agaaaagtgg catgaccatg gataatccag agatctccgt
     6601 cgtaccagtt ccagagactg aaataaagaa aatcacattg aagaacgact acttagaagt
     6661 cgttcttctc aattacggtg cccgcctgca ccaaatcttc gcaccagaca aagaaggaaa
     6721 atcagaaaat gttttattgt cttacgatcg tttcgaagat gttctaacag acaaaagctt
     6781 ttttggagca actgtcggac ctgtagccgg aaggatccgg gatgcttctt ggggtgagca
     6841 tagattggag aaaaactgcg gatcacacca tatccacggt ggcacgaaag gctggtcttt
     6901 ccaattttgg gaagtagaag ttttcaaaac tccatcatca ataggtgtgg tcttttactt
     6961 aaaagatgaa ttttctacct atccaggacc aatcactgct acgatcactt accgtttaac
     7021 aaaaaacgaa ttagagatga caacctctgc tagtagcctt gttgaaacca tctgcaatcc
     7081 gaccaaccat gcctacttca acctttcagg aaatggaaca agagacatct atgaacatca
     7141 gttaacggtc tttttagatg gtgtgcttga attagatcaa gaaaaactgc ctactggaaa
     7201 ttggacaaaa aaagaaaaac tgccaatcga ctttagaaaa tccccaacac tgcaagagat
     7261 ccttgcatgt tatccagatg gactagatga tgtatttctt ttgcatcatc ctaggctttc
     7321 caaaacttcc ttacaattat tcgagaagca ttctggtcgc caaatgacta tagcaacttc
     7381 taataaaagt atggtattat tttctacgac tggattcgaa gctgatttct ctgtcaatgg
     7441 aaaacagatg cattcaaact acggattagc aatcgaaccg caagaaatcc ctgatatcgt
     7501 tcatttcccc aaatttggat cgatcaacct ccatcctggt caagaacgga tcagtcagac
     7561 gatttatcgg ttctctgcac aataacaaga gaaaggtgtt tttatcatgg ctattaccgt
     7621 aaaagatgtg gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg tcatcaacga
     7681 tcatcctagt atttccgaga caacaaagaa gaaagtccga aaaattatgg atgaactagg
     7741 ttatgttcct aacatgactg cccgcaatct aggaaaacgc atttccagtg ccatcggcgt
     7801 aatcttaccc cctcttgatt caaaagagcg gatcggtaac ccattttatc ttgagatcat
     7861 ggaagcaatc aacgaagaag cgcggaatta tgatatgact acagcaatcg ctactgctaa
     7921 aaattttgaa ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac aagtagatgg
     7981 ttttattcta gtgtattcag atagtcaaga tactgtcatt gattatttat accaaaacca
     8041 catccctttc actttgatcg gacagcctta tcatcatgag aacgaaatcg tttatgtaga
     8101 taatgataac cagcttctag gtaaacaagc aacagaattt ttgatccaga atggacataa
     8161 gaatatcctg tttgtgacaa acacgactca tgaaagtttg tatttcgaac gatatttcgg
     8221 ttatcaaaaa gcaatgatga tggctggtct tcctgcgtta tccagtgtag tactagagca
     8281 tccggaggat tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca cagcattggt
     8341 ggtcattgac gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt atggctatgt
     8401 cattccagag acactctccg tcattagctt caacaactcg atcttctcaa cattaacaca
     8461 cccttacctt acaagtatcg atatcgatat ctccgaatta ggacgtgtcg ctatgcaaaa
     8521 gctgcgcgaa ttgatccatg aagaattgag cagtggcgta cgtttggtca tccctcatcg
     8581 cttgatcaaa agagagacag tcgttccttt agaaaaccca tactaaatag ctgactcact
     8641 aaatacgctg accatttatc ctatggtcag ctttttatgt aaaagaagat actacttcta
     8701 acacattgtt tatctgtaaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
     8761 gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata agctgaaatt
     8821 cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag ttaaacgctt
     8881 ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt caataaaaaa
     8941 ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg caagtaatgc
     9001 aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca cggtacgaga
     9061 gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt tgataagcca
     9121 acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag atactgttct
     9181 ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa acgagaaggt
     9241 cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata tttgatcagt
     9301 aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat actcatgatc
     9361 ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac ataaccattc
     9421 atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa agaaagaacg
     9481 agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt gattgccccc
     9541 ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct cgctttcatg
     9601 ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg tcctaatatc
     9661 gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat accagcgata
     9721 tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc tgataaaggc
     9781 gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat tgcatcattg
     9841 aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat cgttgtaaat
     9901 gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg taaaaagaaa
     9961 cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc agataaaatc
    10021 catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat ccctggagtg
    10081 attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa tctgccataa
    10141 aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg gaatccgatg
    10201 atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc ttttaaagaa
    10261 atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca tgaaaaaa
//
LOCUS       DAYYKN010000096        10032 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000096 DAYYKN010000000
VERSION     DAYYKN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.097"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(498..722)
                     /locus_tag="ACHBB7_002075"
     CDS             complement(498..722)
                     /locus_tag="ACHBB7_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210002.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            913..3690
                     /locus_tag="ACHBB7_002076"
     CDS             913..3690
                     /locus_tag="ACHBB7_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9210003.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3771..4190
                     /locus_tag="ACHBB7_002077"
     CDS             3771..4190
                     /locus_tag="ACHBB7_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9210004.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4202..4672
                     /locus_tag="ACHBB7_002078"
     CDS             4202..4672
                     /locus_tag="ACHBB7_002078"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9210005.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4689..5468
                     /locus_tag="ACHBB7_002079"
     CDS             4689..5468
                     /locus_tag="ACHBB7_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9210006.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5455..6276
                     /locus_tag="ACHBB7_002080"
     CDS             5455..6276
                     /locus_tag="ACHBB7_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9210007.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6293..7342
                     /locus_tag="ACHBB7_002081"
     CDS             6293..7342
                     /locus_tag="ACHBB7_002081"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9210008.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7355..8359
                     /locus_tag="ACHBB7_002082"
     CDS             7355..8359
                     /locus_tag="ACHBB7_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9210009.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8992..10011
                     /locus_tag="ACHBB7_002083"
     CDS             8992..10011
                     /locus_tag="ACHBB7_002083"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9210010.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3363 a         1654 c         2093 g         2922 t
ORIGIN      
        1 ttacttcttt tttaatttgt tcttgcaatc ttttttgcta cgtaattttt gtaaaaaagt
       61 gggttgagag aaatctaatt gattttcaaa gaaggagtat gaaaatgaga aaaatattta
      121 ttcgtttatt gtgggtacta tcttagagga ataaataata gtggagaaaa ctatggtttg
      181 gcgcagattc tatcaggttt gatggaacga agacaacctc ccgacatcaa gtcaaaagcc
      241 acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc aatgtttcta
      301 ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca ataaaaagga
      361 tggtgacaac agtacctctt tactctctga aagcatcata actcgttttt cctcattatg
      421 ccaataaatt gttgttatac cacatataag gatttgtaat agctaaaaac agacaggaaa
      481 gttgaaatct gtgaatctta ttttgtatgg aaggatttat agaacgcaaa aatcaagata
      541 aagatgaata aaaatcctgt ttcggctata tattgcgtat ctacgccaat aaaacgagtt
      601 tgttctgtca tagccatata aagcaaaggc aaaaatacaa ctacagcgag ataagatccg
      661 atgatttctc gaaaagtttt ccaggaagta tgtttcctgt ttttatattt atccgatttc
      721 attgaatagt catagcctcc ttattctttc atatgttgaa attccagtat aaaaactgga
      781 aggaactgta cgttttttag aataaaaatt ataagggata agtaggctgc tgtctataat
      841 aagatatatt tttcaattct ttattaaaga ttttattgac aaaatgaatc cgttttctat
      901 gatggaggag gggtgaataa gttgttgaag aaggaaattt tatgttactt agagaaccag
      961 acagcctttt tcgatttaga acatatgaac gaggtcttta cagcacaaag gttagctgat
     1021 cattttgggg taaaaagaaa cacagtcagt cattatttga accagttaaa tgatgagcaa
     1081 ctactagtca aaatcaattc tcgaccagtg atttatttcc acaaagaagc ttttgaaaaa
     1141 caatttttta agttacgcac gaatttttat ttttctatcg atgatctcaa agaggaacag
     1201 ccttttttcg cgcaaccaaa agacttattc tcttttatga tcggatacga cgccagtttg
     1261 aaagaaagta tcgaacaaat caaaacagct ctttactatc cagatggcgg attaccactt
     1321 ttgattacag gcgaaagtgg cacaggtaaa agttatttag ttaatttggt ctatcagtat
     1381 tgtctgcttc atgatctgct agaagattct gcaccattca tcacttttaa ttgtgcacaa
     1441 tatgcagata atcctgaact tttgactagc aatttgtttg gacatgttaa gggagcgtac
     1501 actggtgcag aagaaaataa aaaaggggct tttgaagcag cagatggtgg gatcttgttt
     1561 ttagacgagg tacatcgctt aacaccagaa ggtcaggaaa aactgttcac ctatcttgat
     1621 caaggtgtga tttatcgtat gggagaacct aatttgtcac gccggatcaa aacacgtctc
     1681 ttttttgcta cgactgaaga gattactagt catttcttaa caactttcat tcgaagaata
     1741 cctatccgaa tcgaactacc tgcacttaat cagcgtagtc gtaatgaacg gctagaactg
     1801 atttactctt tttttctcga agaacaaaga aaaatgggga gccctttgtc agtcagtgga
     1861 ccagctttgt cacttttaac aagtcagaaa ttatccggaa acataggaga gctgaaaaat
     1921 atcgtcaaag tttcagcagc aaaagcatat gctgaacagc gtgaacagcc tgaaatccat
     1981 gtcacgatcc atcatttgca aaaagaacta cttgcgcagc ctattactaa aaatacagca
     2041 cagcaaggaa tctatattac gcaagaccag accttagagc aattaatgga gaagcaacag
     2101 cccgaacagc agcgaatcgt taaaagtttt gagcaaatcc taattgattt caagaaaaat
     2161 aactgtctgc tatattcttg cgaaggaaaa ttaaagcaag aagtgattca attatttgat
     2221 tttttgctgt ttgagaccag tagacaagaa aaacacgagc tgctggttta ttggacacaa
     2281 tctatccgag agacctttcg acaaatggaa actgcctatc aaatcaaatt cgatggaaac
     2341 agtgtctatg ccttgagtta ctatctctat cagcgaagca ctatcaaatg gttaccggaa
     2401 gaacatgata ttatccaact aatcaaagaa ttagaacaac aagtttctca gtcttatcca
     2461 tctagttatc actatgtcca acgaattctg gaattaagta agatcaaatt agatattgaa
     2521 gacacaagta tggatcggat tttactgacg atctatctaa aaaaagcgaa atggtcaaaa
     2581 gaaaaacgac tgccaaaagc gatgattgct gctcacggtt atgcaactgc tagcagtatg
     2641 gcaaatgtag taaatcgttt attacaagag gatctatttg aatcatttga tatgccacta
     2701 gatgtcacac cacagcagat tgcttcagaa atccttgatt attgcgaaaa cagcgatgtt
     2761 tcaaatggat tgatcatatt agtagacatg ggatctctga aagaaatcca tcagtttttt
     2821 aaaaaacagt tatccgtacc gcttttgatt ttgaataatg tgacgacacc tttagcaatc
     2881 acagtagggg aatgcctgca aaaaaatgta tcattagaag agatagcaga agagactgtt
     2941 cgacagatcc agccagaatg gcgactgctc tatccagagg aaaacaaacc caaagcactc
     3001 attactactt gcttcactgg aatcggtacg gcggtccact taagtgaact gctagaaaaa
     3061 agcttaccta cggcttgtca gttgaaaatc atcccatatg aatatcagca attaaaagat
     3121 aagaaaaaca gtgacccatt attttccata tacgaagtcg taggaatgat tggcaccaca
     3181 gatcctgata tcaccaatat cgactatctt tcattagaag acctcatctc gggggaaaag
     3241 atgtctgtac tagctgaatg gttggacact actatgaatg cttcagagaa ggaaatattc
     3301 aatcagcgca tcattcgtaa tttttcattg gaaaaagtac tggattccgt gactattcta
     3361 gatacagaga aggtgatggg agaaatcgat cattttatga gagaattaga ggtgcagttg
     3421 aatcgaagca tcagcaatcc caaaaaatta gcgttgtatg tccatgtgag ctgtctcatt
     3481 gaacgattga tccgacaaat gccaatcgaa acctatcaag gattagagaa atggaagcag
     3541 tgtcaaaaag aagggttatc tgctattaaa tctgcgttta gtgtcattga agagcattat
     3601 agtgtcatga tccccaattc tgagattgcc tatatctatg atattttgag tgaagataca
     3661 gagtttctct cgatagaaga agaattttga acataagcaa gttgttgcca ctaattttgg
     3721 catgcaactt gcttttttag tatgtgtaag tgaaaagagg agatgaaaaa gtggatagag
     3781 aaattatctt agcttcccat ggaaaatttg cttcagggat attagattca ttagagctga
     3841 tctatgggaa aaatcattct attactgtat tcgattgcta tactgatgag aattttgatt
     3901 tagcggaaac agtaaggcag ctttttatga ggtacaaaga taaagaaatc attgttctga
     3961 cggatctttt tggcggaagt gtcaacaacg aattcttaca atatatcaat cgggatcatg
     4021 tctatctcgt tgccggattg aatttgccac tattgattga gctagtatct cggttggacg
     4081 ctgaagtaga gacaactgtt ttgatccagc aggtgctaac tgattcaaaa aaaatgatcc
     4141 agttttgtaa cgagcggctc aatcagaaaa tagaagaaga cgaattttag gaggaagaaa
     4201 aatgattgta ttgacaagag tcgaccatcg gttattacac ggacaagtag ctttttcatg
     4261 gacacaaaca ataggcgcag actgtatttt gattgctaat gatgatgtac ctacgaatga
     4321 gatacgaaag acaacgatca aattagccaa gccacaagga gtcaaactag tgatcaaatc
     4381 aattgatgac tcgattgccg cgttgaaaag cggggtgaca gataaatata agttattcat
     4441 cgtagtggaa tcgatcgagg atgcatataa gctggcagaa gcgtatccag aaatcaaaac
     4501 catcaactta ggagggatca aggcaaaacc tggtactcgc agcatcggaa aagcggtaaa
     4561 tattttacca gaagaggagg aacgcttgaa acgattaatt gacaaacaaa tagaagtaga
     4621 aatccgtcaa gtaccagcag ataaaaaagt ggatgtaaga gaagttttgt aagaaaggaa
     4681 gaacctcaat gattgtacaa gcagtcttat taggaattgt tgcatttgtc gcacaatcag
     4741 aatatgcact agggacatcg ttgctttctc gtccgattgt gacggggctg tttacaggaa
     4801 tcgtgttagg tgatgtaaaa actgggatca ttatgggcgc aactttagag ttagcattta
     4861 tcggatcgtt ctcagtaggt gcttcgattc cacctgatgt tgtgaccggt gggattttag
     4921 ggacggcttt tgctattaca gctggagccg gtacagaaac cgcgctttta ctaggtttgc
     4981 cgattgctac gttgacattg atattgaaaa atatttactt agggcttttg attccaatta
     5041 tgaaccataa agcagacaat tatgcagaag aaggaaatta taaaggagtc gagcatatgc
     5101 acttgcttgc cggtatcggt ctttccttga tgcttggtgt tgtggtaatg gtttcttata
     5161 tggtgggaag caatacgatc ggaaatctat taaatatgat tcctgacttc gttcaaaagg
     5221 ggttgtcagt agctacaggg ttgattccag ctcttggttt tgcgatgctt gctcgtttgc
     5281 tgatcaacaa acaagttgca ccatattttt tcttaggttt tgctatagct tcttacttag
     5341 agattcctgt gaccggagta gctatttttg gtgcgatttt agcagttgtg gtagtcaata
     5401 ttatgaatgt ccgtcaacta ccacttcaaa caactgcagg ggaggtagaa gaagatgaag
     5461 aattctgaga tcaaaaacag caaaataacg aaaaaagagc taaatcacgt attttggcgt
     5521 tcttttcaaa tggagttttc ttggaattat gaacgtcaga tgaatatggc ttatgtttat
     5581 gcaatgattc cgattttgaa aaagttatat cagtcgaaag aagaaatggc agcagctttg
     5641 aaacggcatc tagagttttt taacacgacc ccacatatcg ttacactgat tttaggaatc
     5701 acggctgcaa tggaggaaga aaataaggat gatcctgaat ttgatgtgac tgcgatcgat
     5761 agtatcaaaa catctttgat gggacctctt gcagggttag gagattcttt cttttgggga
     5821 actttgcgat tgattgccac aggtgtgggt acctctttag ccatgcaagg aaatatttta
     5881 ggccctatct tgttcatttt gattttcaat attccgcaca ttctgtttcg ctatctagca
     5941 acaggttggg gatatcgctt aggaacgggt tttttgaaaa agatccaggc aaacggtatg
     6001 atggaaagtt tgacattagg cgcgtctatt attggactga tggtcgttgg agcaatgact
     6061 gccacgatga tcaccatcaa tattccttta aaaatcggtt caggagaaaa tgctgtgact
     6121 gttcaaagta tttttgatga tatcgttcca aatatcttat gtcttggagc atttggcata
     6181 gtgttttatc tattgaagaa agaagtgaaa ccattaacga tcttgatcgg actagctttc
     6241 tttggaattt ttggttcgtg gatagggatt ttttaaaaaa ggagcagaga aaatgtttac
     6301 gatgcaagat tatatctatg aagaaaagga agtactaagt acgatcttga aaaagaatga
     6361 tttttcgaca agagaacata tgaaaaaaac agtgaattta ctgatcttag caacgggttc
     6421 atcttataac gcttgtttag cagcgaagcc agcattggaa tcttatggtg atcttactgt
     6481 cgatattcaa gaaccttttt attttttcaa ttatggaaaa ctttctcctt caatcgatac
     6541 tgtgattgct gtgtcacaaa gcgggaaaag tgcttctact gttgaagcag taaaaatgat
     6601 acaaaaacaa gggttacctg ttgttgctat cacaaatgac gtgcaaagtc cacttgcttt
     6661 agaagctgca caaattattg atttaggtgc cggggtagaa tcagtaggct tcgttacaaa
     6721 aggctattct gcgactgttt tacagttact actcttaggg ataggaattg gaataagtaa
     6781 aaataaaatc agcaaaaaaa tagaacaaga ctacatgcaa cagttgcgca aaataatcaa
     6841 ccatttacca gcgattattc aaaaaacgga agaatttttt gatggatatc aaagtttatt
     6901 tcgtttagca cagcgtttcg tcaccattgg atatggtcca aactggggaa cagctaaaga
     6961 agcagagacg aaattgactg aaacaatccg cgttccgtca caaggatttg agttagaagc
     7021 ttatatgcac ggcccatacc tggaggcaga tgcttcgcat ttgctattct ttattgaagg
     7081 ggaaagtgta aataaggaac gttcgcaaaa gttgcaaaga tatatgagtc ggtacgttgg
     7141 agagactctc accattacca caaaaaaagc aagaaacgag aaaacacttg gattagcaat
     7201 agaatgtgat gaatatctgt ctgtattagc gcttgtcgtt ccgtttcaac tattcgctta
     7261 taaaaccgca gtagctaaag gtattgactt gaacaaaaag atttttgaag actttgatac
     7321 agtcttgaaa agtaaattat aagaggtgga aatcatgctg aaattcaatg aagaagaaca
     7381 aatcaaagca ataaaaggcg cactagcttt acgtccgcaa gtagaagaaa tcatcgataa
     7441 actttatgcg gaaaaatttg acgctgtcta ttatctagga atcggtggaa cgtatgcttc
     7501 ctcgatgcaa gctgtcactt atatgaatgg gaaaagcaac ttgcctgtct ttgtacagca
     7561 tgctgcagaa tattacacta ctggaaacaa aagattaaca aaagattcta tcgttatttt
     7621 gtcctctgtt accggtacaa ctcaagaagt agtgaaagca gtagaacaaa tcaagaaagt
     7681 aggagcgaca ctgattggat ttatcgataa agcgaacagt aagttgtctc agctatgcga
     7741 ttttgtggtc acttatccag ctcctggaac agaacaaatc aaatttttta tggcagcaga
     7801 tcgattgatg tatttgaatg gagaattcga agcttattca gaatactatg aacagctcga
     7861 acgttattta ccaacagggt tagtggaagc agagaaaaaa gcagatgcat ttggcttatc
     7921 atttgcagaa aaacatcgac gtgatagtat gcattatttt attggagcag ggaatcaatg
     7981 gggagctgtt tattcatatg ccatgtgtta ctgggaagaa caaagctggc ttccatcgaa
     8041 atctatccat gctgccgagt ttcttcacgg gacgttagag atcgtagaag aaacaacccc
     8101 agtcacatta tttttaggcg aagatgaaca gagagtgtta tctgaacgtg tggctaaact
     8161 tttgccaaaa atttgcagca attatacatt gatagacaca aaagactatc cagtagaagg
     8221 tatcagtgaa aaatatcgcg gacgtgtttt gtctttctta ttgatgcatg tagtgacaca
     8281 acgcatcgat gctcatgtgg agcagttgaa ctgccatcca ttagagatac gccgctacta
     8341 tcgtcagttt gattattagg atagactgga taaaaagaat ataaaaaata aagagagcag
     8401 cgataactgc tccctttatt tttttaaaat attggcatca attacgttat aaaacacatt
     8461 ttttgcatta tctacagttc aacttataat ctgtttaatg ggacatgtgg gtgccaacca
     8521 ttttttgtta tgcaatagta ctatttagtt gttgcatgga tccgtagtaa aagtccaagt
     8581 acaacgagtt gcgccagttt acatagcgtt ttgtatccag aatgttgact aaaacaaaag
     8641 ttcctatgtc aaccgtcaac agagacaatc tgtccgttgc aggacctgac agatttagaa
     8701 atgttcaatc tgtctcttat gtattttaaa tcaatactca ctaggagaag tttcctaacc
     8761 tcctaatagt aatgctggta gtaacattgt caaaactgct tcctcttagg aacaagcaaa
     8821 gcataacgag tgtaaaaata aaatgtagac tattgtaaca tatcattgat tatttcaagt
     8881 aaaaacgata aaagaagtaa acaaacactt aaaaacaatg gaaattagtc ccaactttaa
     8941 gaaatgtata ctataatgga aattatcaat tatttatttg gaggaatatc catgaaacaa
     9001 aaaatcgcag ttcttggtcc tggatcttgg ggcactgctt tagcacaaac attggcagaa
     9061 aacgggcatg atgtccgtat ttggggaaat gtacctgaac agattgatga aatcaataag
     9121 tatcatacaa atcagcattt tttacctgat ttgacgatcc cagaatcgat tatcggatat
     9181 aaagaattgg cagaagcagt ggataacgca gatgctattt tatttgttgt tccaacaaaa
     9241 gcgattcgtt cagtcgcaca ggaacttatt ggtaaaatga atactaagcc aatcattata
     9301 catgcaagta aaggactaga acaagatacg cataaacgaa tctcagaagt tttggctgaa
     9361 gaaattccag aagaaaaaag acaggcaatt gttgtattat ctggtcctag ccatgctgag
     9421 gaagtggcag tacatgatat cacaacaatt acagctgcaa gtattgatca aaaagcagca
     9481 gcctatgtac aaaaactgtt catgaatgat tatttcagga tttatacaaa taatgatgtg
     9541 attggcgtag aaactggtgc agcactgaaa aatatcattg caattggtgc cggcgctatc
     9601 catggattag gattcggaga tgatgcaaaa gcagcaatca tgactcgagg attggcagaa
     9661 atcagccgtc ttggtgtcgc gatgggtgcc aatcctctga catttatcgg tttgagtggt
     9721 gtgggggact tgatcgttac ttgtacaagt gttcattctc gcaattggcg tgccggtaac
     9781 ttgttaggac agggacataa gctggaagaa gttctagaaa acatgggaat ggtcgttgag
     9841 ggtgtggcaa cgacaaaagc agcagtagag ctagctcaac agctaggtgt tgagatgcca
     9901 attactcaaa caatctataa tgttttgtat aatggagaag atatcaaaaa agcagcaaaa
     9961 gaaatcatgt tacgtgacgg aaaaatggag aatgaatttt ctcttcgtta aatagatgat
    10021 caatagttaa tc
//
LOCUS       DAYYKN010000097         9990 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000097 DAYYKN010000000
VERSION     DAYYKN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.098"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..325)
                     /locus_tag="ACHBB7_002084"
     CDS             complement(<1..325)
                     /locus_tag="ACHBB7_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM9210011.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLE"
     gene            complement(343..663)
                     /locus_tag="ACHBB7_002085"
     CDS             complement(343..663)
                     /locus_tag="ACHBB7_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9210012.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            957..1097
                     /locus_tag="ACHBB7_002086"
     CDS             957..1097
                     /locus_tag="ACHBB7_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210013.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            1395..1652
                     /locus_tag="ACHBB7_002087"
     CDS             1395..1652
                     /locus_tag="ACHBB7_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFM9210014.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(1623..1931)
                     /locus_tag="ACHBB7_002088"
     CDS             complement(1623..1931)
                     /locus_tag="ACHBB7_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210015.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            2009..2755
                     /locus_tag="ACHBB7_002089"
     CDS             2009..2755
                     /locus_tag="ACHBB7_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM9210016.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            2770..2973
                     /locus_tag="ACHBB7_002090"
     CDS             2770..2973
                     /locus_tag="ACHBB7_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210017.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            2989..3327
                     /locus_tag="ACHBB7_002091"
     CDS             2989..3327
                     /locus_tag="ACHBB7_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM9210018.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            3314..3493
                     /locus_tag="ACHBB7_002092"
     CDS             3314..3493
                     /locus_tag="ACHBB7_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210019.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(3536..4006)
                     /locus_tag="ACHBB7_002093"
     CDS             complement(3536..4006)
                     /locus_tag="ACHBB7_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210020.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            4093..4791
                     /locus_tag="ACHBB7_002094"
     CDS             4093..4791
                     /locus_tag="ACHBB7_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM9210021.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4969..5310
                     /locus_tag="ACHBB7_002095"
     CDS             4969..5310
                     /locus_tag="ACHBB7_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210022.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            5303..5974
                     /locus_tag="ACHBB7_002096"
     CDS             5303..5974
                     /locus_tag="ACHBB7_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM9210023.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            5980..6672
                     /locus_tag="ACHBB7_002097"
     CDS             5980..6672
                     /locus_tag="ACHBB7_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM9210024.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            6669..7418
                     /locus_tag="ACHBB7_002098"
     CDS             6669..7418
                     /locus_tag="ACHBB7_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210025.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            7430..7699
                     /locus_tag="ACHBB7_002099"
     CDS             7430..7699
                     /locus_tag="ACHBB7_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210026.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            7705..7869
                     /locus_tag="ACHBB7_002100"
     CDS             7705..7869
                     /locus_tag="ACHBB7_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210027.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            7862..8161
                     /locus_tag="ACHBB7_002101"
     CDS             7862..8161
                     /locus_tag="ACHBB7_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210028.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            8161..8463
                     /locus_tag="ACHBB7_002102"
     CDS             8161..8463
                     /locus_tag="ACHBB7_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM9210029.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            8503..8868
                     /locus_tag="ACHBB7_002103"
     CDS             8503..8868
                     /locus_tag="ACHBB7_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210030.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            9358..9498
                     /locus_tag="ACHBB7_002104"
     CDS             9358..9498
                     /locus_tag="ACHBB7_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210031.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            9495..9677
                     /locus_tag="ACHBB7_002105"
     CDS             9495..9677
                     /locus_tag="ACHBB7_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210032.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            9690..9884
                     /locus_tag="ACHBB7_002106"
     CDS             9690..9884
                     /locus_tag="ACHBB7_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210033.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT         3638 a         1586 c         1994 g         2772 t
ORIGIN      
        1 tttcgagatt aaactgagat tcatcagtca gttcgttctc aatatagata tttaataaaa
       61 ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt gtagaagtat
      121 cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg tgcaagttca tgagataaat
      181 gaaaggcttc tgcagtttcg ccatatagat tttcattcaa aaaaatgatt ctaggcttcg
      241 gataatagaa gccaggctct ttcatttcca tgtaaactac tttcaaattg tattcgctca
      301 acatttcttt caatttcaaa tacatacaac ccatcactcc aactattcat tttcctctaa
      361 agctttagcg attgcaatcg ctttacgcat tgtctcctta gatatttctt ttccatcaaa
      421 agaaaaaaca gtatcgtttt ctgataaatc cacatgttta ggggtttctt tttcttcgcg
      481 ccccagaaga tagtctacag agacatcgaa atagtcagca atttcttgca aacgttctgt
      541 agaggggttt gagttcttaa gtctatataa gacgttttta ccatagccta ggtcttcttc
      601 aactttattt aatgattttc cctgtttttt cgctaactct tttattctct caaacgttat
      661 catatcaata ttcctttcgt tcaagaaaaa atatttaact ttatttgtta aaattcgttg
      721 actattttaa caaaaggtgt taatatcatt cttgtaaaca agtttaacaa ctaaaaagac
      781 aacaaaaaac actattgatt aataaatgcc aaccgccaag aaagctttta aaatcaatgt
      841 ttatatgtct tatttaacta tgttctgatt ttaacactgt atgttaattt agtcaacgca
      901 gttttttaaa aagttgttaa atttgtttac gaatataaaa gaaaggagag aagaatatgg
      961 aaaaaacagt ctcggcaaaa atcgaagatt tgaaattaga catactgaaa caagcaaaag
     1021 tggcgatgga acacgcggta gataaagaag actctgccat ggttgcagcc atagcagaga
     1081 ttttagctca cgtttagtca tcgtttttac ggtcttctgg aagctgacca tagataacag
     1141 cataatgaat atctaaataa gtttgaacaa tttcttttgg cgaaattgat tcgcctttag
     1201 tagatacttg tgattcgtga tttagccatg caacaactaa atccgcagcg atttcaaacg
     1261 gcgttacact ttgtgtcagt tgccataaag aatttcacaa tatgtatggt cgtggtaata
     1321 ataccaaaga gcaatttgag gaatttatgg aaattaaaaa atacaaaaaa tcatttatta
     1381 aaggaggcta aacaatgaac aaggaaatga gccaaacaat caaggtacaa aaaatgatag
     1441 atgatctaac acatggaatt gatagccaag ccgataaaat catcaaggaa ttgcaaggac
     1501 aaaaagttaa ggatgcaaaa atgcttctca agactatcaa ctttgaaatg aatccaacaa
     1561 aaagaaagct cgccgatgtg ttggaagaaa agttggcttc cgctatcaac gagcaagaat
     1621 tactatttga aaccgatact tttaacagct gatgttttat agatgacagt ttcatccgaa
     1681 tctatatcat cgccgagaga aaagaatgtt gcttttgaca aaaagccgat aagacctaca
     1741 agcggtcttg atgttgcaag agtagatacc ccaatatcac cttgcaaggt attcacgtaa
     1801 atttgagaag aatagaacat tccatctttt tcttcgatat ttgacgcatc atcgtatcca
     1861 acgaccaaag tatcggcaaa tattttgatt ttttctccat caagcatagt aattacaaat
     1921 tctggtttca taaattttca cctccttaac aattatttca gcctgtcaca ctgataagga
     1981 aattatacca aagaaaggaa tgagaaatat gaatacacca caaattttta atttcgagca
     2041 aaacgaagtt cgaactattt tagtaaatga tgaaccatat tttgtaggaa aagatgtagc
     2101 agacgttcta ggatattcga atccgcaaaa agctattcga gatcatgtgg acttggagga
     2161 taagacgcag aacgattcgt tcaccgtcaa tggaacagca gttgttttga ttaacgagtc
     2221 aggcctttac agtttaatct taaaatcaaa acttccctct gccaaaaaat ttaaacgttg
     2281 ggtaacgagt gaagtccttc caacaattag aaaaacaggt agctattcaa acgtacctca
     2341 aagttttgca caagcattgc gtttagcagc ggatttagaa gaaaagaacc aattactcga
     2401 acaacaaatt gccgagtacg aaccaaagat tagctactta gatacgattc tttcatcgac
     2461 agatacggta gctacttctc aaattgcagc tgattacgga atgtcggcaa ttgctctaaa
     2521 caaattgcta aacgagttag gtgttcagca taaagttagc ggacaatgga tactttaccg
     2581 aaaacatatg aaccaaggat acacaaaatc gcacacaagt gagataccga aagccgatgg
     2641 cggcactaaa gttgtaatga ataccaaatg gacacagaaa gggcgagtgt ttatttacaa
     2701 cttattaatc gcagagggct attaccctca aatggattta gaggaaattg gttagaaagg
     2761 agttttagta tgactgacat tgcagaaatc actcaacgag atagagaaaa aatcaaagaa
     2821 tatgtcgaaa gttcaaagtt cttaacttac accatgcttg ctgaaagatt tggaattagc
     2881 aaaagctact tatctttaat tttaaacggt aaaaagactt ctgcagaagc aaacagaatt
     2941 atagattcga ttatcactat gtacgaattg taaagggagg aacagctaat gcaatatcta
     3001 gaagcaaaaa tcccaattcc agaaggctat gtaattatct cccaagtgga ttatgaggag
     3061 ttaaaaaaag ctgatgatac tggtagatgg atgacgttgc cagaagtgct agaacggatt
     3121 aacagaaaat atgattggtt tacttctaga gttttaaaga acccaagata tagaaatatt
     3181 atcgatatcg agaaaaacaa aaatggattt gtctattatc cagttgaagg aagagacaca
     3241 tatctatttt taagaagtaa aacacttgaa tttttagaaa caaatttttc ggaaatctta
     3301 aggaggcaag cggatgggaa aatttaatag agcgctagtg ttcagcgcac cgctaatcat
     3361 ctacgcttta ggactttggg gaagcaggca agcgttgata ggaacgattg tttacatggt
     3421 ttggattttt atggggcttg atgaagctga agctgagtac agagcgaaaa agccaaccga
     3481 gggggctgac taaaaatgaa tagaaaagag aaactagaat gtatattact attactcagt
     3541 ttaattcttt cactaatttc tctattggga agtttgtatt tttgatatca acaattttgt
     3601 ggatttgggt tctatttaga gtaaatatca tttttgtttg gtgattaagg agtttattaa
     3661 aattaatttt ccctacttca aatgcaaggt aacaagatat agcactttta ggaggaatat
     3721 ttagcggtac agcttcagag tatattctat tatcatcgac tgatcctcct ttagcaatta
     3781 atttcttaaa tggagtagct gaccatctgc ggtttaagtt agcggatgaa agttctaaat
     3841 taactaacat agctggttct gttgaatagt tagaaatgat tacttttgta taaatcatat
     3901 ctgcagcgaa gtaagaagca ttaagttcaa cttctagttg agggcgcatt ccttttattt
     3961 ttatccctgt taaaaacgta ctcaatataa aacttattat tgacatccat tgaaaaattg
     4021 ttaagtgaaa aaaattcaaa ataaacacca ccagttttta actaaattat accaaaaagg
     4081 agagaagaaa taatgcaaga attagtaatt ttgaaaaata aagaagctgt gactacgagc
     4141 ttacaagtgg cagaaagctt cgagaaaaaa catcaacatg ttttaagaga tattgatgca
     4201 ctaaaaaaag atgtgtccaa ttttggacag atgtttgtag aaggtaatga accagattca
     4261 tatggcagaa atcgacgagt tttcttcatt agtagagatg gatttttctt gctggctatg
     4321 gggtttacag gaaagaaagc tatctacttc aaacaaaaat acattgaagc attcaacgaa
     4381 atggaagatg ttattcgcaa gaatactgtt cctcaaacaa ttgaagatat gatgatctat
     4441 caattagaag aaatgaaaga tgttaaaaaa gatgtttcca tgcttaaaga tactatgcga
     4501 attagcggac aacaagagtt tgaaattaag caaaaaggaa atatgaaagt tatgggagtt
     4561 ctagggggaa aagaaagccg agcttatgaa gaaatcagca aaaaagtatt ctcaaaattt
     4621 tggtctgaat ttaaacgtac cttttcaatc ccaagatatg gcgagttacc tcgtaagaga
     4681 ttcgatgatg ctgtttcatt tattgaaatg tggttgccag aaactgcgat ccgtatggaa
     4741 attgatcaac tgaacagaca acagagactt tttggtgatg acaatgaata gagctgaagc
     4801 gctaagaata gggacggcaa ttgctaattg ctggtggaaa tactataaac caatcatcct
     4861 aagccaacaa catattgaca agcaaaaagc atggcaacaa ataaaaaagt gactccgccg
     4921 gcaagcaaag agtcacaaag aaaacacatc ataaggagat tttagcatat ggaaaaagaa
     4981 ctttccactc tagatcaata tttgattgat cctgattggg gcaagccgaa aattgaggaa
     5041 acaagtggtc gaaaaatcag acgaaatctt ttgacgaatg aagaactagc ttgggatcaa
     5101 gatgatttag gcaaccatgt aactatttgg gatcatgttt atcttatcca tctatcgaag
     5161 cattcgaata aacctgaata tatttacgtc atcgaagatg gcttgattga tgcgctagaa
     5221 gagtacgaca gagataactt gattgatatc tcttattacg gaccaggtaa gaaatacatt
     5281 gctgaaatgg aggcagaatt tgatgagtga aatcaaaggg acaacgaact ttgaaaaact
     5341 ttttagtcgt aagttaaata aaattctcaa gaaaaaagga aattttgatt atttatcttg
     5401 ggctcacgcg tgggagatta tgaaaaagaa tgatccacag gcaacggtaa ctattaatga
     5461 gtacaaacac tacagggttg tttctggaac tcatcaagac tttcttgttg aggaatataa
     5521 accttttctt atggatgaaa ctgggactta tgtatctgtc tcagtaacgg ttaaaggaca
     5581 cacggaaacc gaattatttc ctgttttaga ttatcgaaac caaccagttg ttaaaccaaa
     5641 tgcaatgcaa atcaataact cattgaagcg atgctttgtg aaagcattgg ctctacacgg
     5701 actgggatta tatgtatttc aaggggaaga tattccaaca ccacctagaa tcgatacaaa
     5761 gaaattaaac atgctagaga cgattctaga agctttcaat gagcagatgg gtaaagatat
     5821 gaccaaaacc ttaatcgaat atgttaatga gcagacagat aaattagggc tcttagctga
     5881 taacgttgaa actattgaac agttaagcta tgagcaatgt gccttgatgg agcgagcaat
     5941 agcagctaag agaaaagaat tagataagaa gtgatatgag tgtttaaacc attaatcgat
     6001 tcatattcag cggttctgaa aaagttcaaa ggaaaagaca taagcgcaac catcaatgag
     6061 gaagtgaaca ttgatcgact aaagacgatg tatgacggct acgatggtga tcgagtcatt
     6121 gaaattcgtt ttattgatcc tagacgtttc accgtacagc aacgaaactt catctatgcg
     6181 ctgataggcg atatttttat cgatacaggc atgccaacgg acttctggaa ggaattcttc
     6241 tactttcgtt ttgaaggtgt cacagggcgc aaaataagcc tgaaagacga atcgaataca
     6301 actgtgagtg atgccaatgt cttagcaaat atcatcttag atttcatctt tgaacatcat
     6361 attcctttca aagaaggcta tgagatttta cctgcgaatc aagaatatta cttctacaaa
     6421 tgcatcacaa aaagagtctg ctgcatctgt ggcaaaacag gagctgacat cgatcacttt
     6481 gacaaagcgc taggaagacg aaagcgcaaa gaagttgatc atgcagagta cacatttgca
     6541 gcactctgca gaatccatca cacagagaag cacaaaatag gtgtgattaa tttcaaaaat
     6601 aaatatcaaa tcaaagggat caaattaaac cagaaaacaa tcaaaaagtt aaatattgga
     6661 ggggaaaaat gacagaacat cgaagttatt atgcgattat accagccaac gtaaggtacg
     6721 acaaaagact taaaccaaat actaagttgt tatacggaga gataacggcc ttgtgtaatg
     6781 aaagaggctt ttgttgggca ggcaatgagt actttgcaga tttatatggt gtgaataaag
     6841 agaccatatc gcgatgggta agtgatttga ttaagtttgg atacttgaat cgggaaatca
     6901 tttacaaaga gggtaccaat caaataatca ataggtacct acgaattaat caatacccta
     6961 ttgacgaaaa acgcaatacc cctattgacg aaaaagtcaa agataataat acatctatta
     7021 ataatacatt taataataca aaagaatata taagagagtt accgccttcg aaaaaatcga
     7081 aggctaagcc cgtccgtcat aaatacggag agtataaaaa tgttcttttg tcagatgagc
     7141 agatggagaa actcaaaaca gaattcccta atgattacca agagcgaatc gaacgactgt
     7201 cagagtattg tgaatcatct ggtaagactt ataaaaacta tttggcaact attcgaagtt
     7261 gggcaaggaa agaaaaaagt gaacctaaga acgcaagcag tggatacaag cgcacaggaa
     7321 gacgagagaa gcttcctgaa tgggcaatcg accaagaagc ctatctcaag aaaaaagcgc
     7381 tagaacgagc taatagacaa tcaaaagcac cattctaaga ggtggaaaaa tgaagatcga
     7441 ttatctagaa ctaattaatg aaatagcgaa ttataaaaag ggcgaggaat tagacgtcct
     7501 gagagacgta tatgatcaac tcgaagaagc tggaattgaa ggaattaaga atgatcgttc
     7561 gagttggagt aaactcagat actatttcgc actctatatc gatggaacgc aattaagaaa
     7621 tttagcatat accaaattac tattcattga ttgtgttaaa ggcttgcaaa aacatcttaa
     7681 tgaacttgag caggtgtaaa caagatggac ctaaagacat ttacagcaca gatcgaatta
     7741 atgcatcaag aagctttaag acaaagtgtg tcgtacgaag acaagtggct caacacattt
     7801 catggcggac gtgagagcgc acttgatcaa gtactcaaat tactgaaagg agaatgtcgg
     7861 gatggataag aaagcggcaa tgcagcgaat tatcgaattg acttattcag aagattggca
     7921 ggaagataaa aaaatagttg cagaagtcca aaagctcggt aaatcaatgt ggactgaaaa
     7981 gcccaaacgg agaacgccga gaaagattgc aatctggcat ggtgatcgaa ttctagtaac
     8041 gggtaccgct aaacagttat ctgaaattac tggtctgagc aaaaatatta tttgggatag
     8101 agctaggagc ttatggattg attcaaaagg aagacagttt aggtatgtgg aggagaaata
     8161 atggacgaac taatcgcgaa agtagagcag tgggcaaaag ataagggatt ggatcaagcg
     8221 gatttcagca agcaaatgtt aaaaacggtc gaagaaatcg gggaagttgc cgcttctcta
     8281 gctagaaaag atgaacatgg tttaagagac ggaattggcg atgtagtagt aaccttgatc
     8341 atcttggcaa tgcaaaatga tatggattta tacgagtgct tggattttgc ttatgacgaa
     8401 atcaaaggtc ggactggaaa gatggtcaat ggtgttttcg ttaagtccag tgacctgaaa
     8461 taattccatt aatggaagaa ataactgaat aggaggatag acatgattga tgcaaaacaa
     8521 gtgcaaaaac agaaagatgg aatgttgatg tttgaagcat atgttctacc atttcttaat
     8581 caatttgaag ttttggaatg ctctgccagc ggagaagaat tggagtatgt tgtaattcgt
     8641 gaaaccaaag aaaacgttca gaagctaaac gaatttcttt gcactattaa ctgttgggat
     8701 atgatagctc caggattttt atgtccagct atgggagaat ttttggaata ttgtcgtctt
     8761 gaagatgcag gcactttgga tttagcttat ctggtataca actacctaaa catcaacaca
     8821 gatcatttgt ggtttggcac tgccgaaaga aaatgggtag tccgctaacc accaatatca
     8881 atgaaaaaag gcggtttcct gaaagtggaa ttgccgccat tgctgtaagg ttctttacgc
     8941 gactcaaggt cacagaaata taaatgcaaa tctcttttga agtgatccaa agctctcgga
     9001 aaatggaagt tttttgtttc aaagaataaa tgagatttgc gtcaagaacc tgagagttat
     9061 ttccaaaaaa gcttccaaag aaccgaatga gctaaggaca tcatgtgtaa acacataata
     9121 tagctctcct ttcacggcta atcataacat gaaaaatatt cgatgtcagt aggaaaaaag
     9181 gtgaattatg aaaagaatat taaattatcc cggcagtaac agattctgca attaaactcc
     9241 gaagctgatg tatcagtatc aactgtagaa tattatgagc gtggagaaag tcaagcagtc
     9301 acaaatgtaa aaatcgataa cgtcaacggt cttatctgtt tagatgaagg cgatagtatg
     9361 aatgaaattg ctgcagacca tgaattatca gaaactgctt taattgatca aatcgaatta
     9421 gctagacaat actttgttcc atatgcagat acctggtcca agcataagaa tgacatcatt
     9481 tttccagagg aacaatgaga gcaactgatc cagtaattat ccttgaggaa gccaaattta
     9541 tttggactca cgaagagata gagcaagcac gcttgctctt ttctcaagga gttaagccga
     9601 gcaaagtagc tgaaataatg ggtcaaaaga ttcttgatgt cggattgctt ttgctccatc
     9661 tagcagaaaa aatttgattt gaggtgaaaa tgatgattca acttgcaggc atccaaacag
     9721 ggaaaattta tttttctgga gaaagcaaaa gcgaagccaa ccaatggcta ttgaaaactt
     9781 acacgaacaa taagaagcta aggaaaaaat atcctgattt gttgctaaag gatgatcaga
     9841 ttatgccgga accgatgatt ttgacccgta aggagggaag ttgaaactaa aaaaccagcc
     9901 gtgtgggctg gagttgctat ttctttttaa taatgagttt ttctaaattt aattcattct
     9961 cacgagtttg cgtccaataa tcagaataat
//
LOCUS       DAYYKN010000098         9899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000098 DAYYKN010000000
VERSION     DAYYKN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.099"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..572)
                     /locus_tag="ACHBB7_002107"
     CDS             complement(60..572)
                     /locus_tag="ACHBB7_002107"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM9210034.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(586..2883)
                     /gene="recJ"
                     /locus_tag="ACHBB7_002108"
     CDS             complement(586..2883)
                     /gene="recJ"
                     /locus_tag="ACHBB7_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM9210035.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3025..3468)
                     /locus_tag="ACHBB7_002109"
     CDS             complement(3025..3468)
                     /locus_tag="ACHBB7_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM9210036.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3469..4257)
                     /locus_tag="ACHBB7_002110"
     CDS             complement(3469..4257)
                     /locus_tag="ACHBB7_002110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9210037.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4254..5195)
                     /gene="rnz"
                     /locus_tag="ACHBB7_002111"
     CDS             complement(4254..5195)
                     /gene="rnz"
                     /locus_tag="ACHBB7_002111"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM9210038.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5372..5959)
                     /locus_tag="ACHBB7_002112"
     CDS             complement(5372..5959)
                     /locus_tag="ACHBB7_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM9210039.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6180..7493)
                     /gene="obgE"
                     /locus_tag="ACHBB7_002113"
     CDS             complement(6180..7493)
                     /gene="obgE"
                     /locus_tag="ACHBB7_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM9210040.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7781..8791
                     /gene="gap"
                     /locus_tag="ACHBB7_002114"
     CDS             7781..8791
                     /gene="gap"
                     /locus_tag="ACHBB7_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM9210041.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8876..9811)
                     /locus_tag="ACHBB7_002115"
     CDS             complement(8876..9811)
                     /locus_tag="ACHBB7_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9210042.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2722 a         2268 c         1711 g         3198 t
ORIGIN      
        1 agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct
       61 taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc
      121 atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg
      181 atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga
      241 cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa
      301 gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact
      361 cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc
      421 atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc ccgataagcg
      481 tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg
      541 ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca
      601 tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag
      661 ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg
      721 taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat
      781 taataatttt tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc
      841 aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc ttgtgcctac
      901 cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat tgaatgaata
      961 agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa caactagaga
     1021 gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc
     1081 agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag
     1141 gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg
     1201 cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc
     1261 attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt
     1321 accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga caaatttcag
     1381 atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa
     1441 ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc
     1501 tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc ctctcatcaa
     1561 gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc
     1621 acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg
     1681 catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc
     1741 tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt
     1801 cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg
     1861 attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct
     1921 ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt
     1981 cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg
     2041 agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc
     2101 aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc ctaatgaaac
     2161 aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa
     2221 atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga atgctactcc
     2281 gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc
     2341 ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc
     2401 agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat
     2461 caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc catacccatg
     2521 ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt
     2581 cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc
     2641 tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt gtatcagata
     2701 cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg
     2761 gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat
     2821 ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct
     2881 cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa
     2941 gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt
     3001 acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg
     3061 agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc
     3121 cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc
     3181 ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag
     3241 ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag
     3301 tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa
     3361 gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac
     3421 taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt
     3481 aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc
     3541 aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat
     3601 catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc
     3661 gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca ctgtcgtaac
     3721 agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa
     3781 tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca tagaggcgat
     3841 attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac
     3901 catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag
     3961 gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc
     4021 ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa
     4081 agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc
     4141 cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg
     4201 tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc
     4261 tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt
     4321 tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca
     4381 ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga
     4441 tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt
     4501 tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta
     4561 tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg
     4621 atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt
     4681 ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt
     4741 tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa
     4801 aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa
     4861 cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc
     4921 ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga
     4981 agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga
     5041 cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact
     5101 tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga
     5161 acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc
     5221 ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa
     5281 ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc
     5341 gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt
     5401 cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat
     5461 tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgagtaa
     5521 tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag
     5581 caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg
     5641 tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc
     5701 atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg attaatccac
     5761 ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg
     5821 tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat
     5881 gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa
     5941 tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc
     6001 tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac
     6061 aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct
     6121 gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc
     6181 tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg
     6241 cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact
     6301 gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag
     6361 atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca
     6421 tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct
     6481 aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt
     6541 ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg
     6601 aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc
     6661 ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct
     6721 tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt
     6781 ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc
     6841 aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga
     6901 ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg
     6961 gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact
     7021 ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc aatttccggt
     7081 gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc
     7141 acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct
     7201 cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc
     7261 cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc
     7321 atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct
     7381 ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct
     7441 ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag
     7501 tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt
     7561 acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct
     7621 tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt
     7681 tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat
     7741 gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat
     7801 taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga
     7861 tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca
     7921 atttgactct actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt
     7981 agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc
     8041 aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa
     8101 agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc
     8161 tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat
     8221 ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga
     8281 atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct
     8341 cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac
     8401 tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg
     8461 aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt
     8521 ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac
     8581 agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg
     8641 aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt
     8701 ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat
     8761 tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct
     8821 ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat
     8881 gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt
     8941 atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac
     9001 gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga
     9061 gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc
     9121 cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa
     9181 acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa
     9241 tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc
     9301 ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct agcactttgt
     9361 ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc
     9421 tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct
     9481 ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt
     9541 tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag
     9601 tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt
     9661 tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa
     9721 cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga
     9781 cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca
     9841 tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAYYKN010000099         9870 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000099 DAYYKN010000000
VERSION     DAYYKN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.100"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..263)
                     /locus_tag="ACHBB7_002116"
     CDS             complement(18..263)
                     /locus_tag="ACHBB7_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM9210043.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(378..956)
                     /locus_tag="ACHBB7_002117"
     CDS             complement(378..956)
                     /locus_tag="ACHBB7_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9210044.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1033..1563)
                     /locus_tag="ACHBB7_002118"
     CDS             complement(1033..1563)
                     /locus_tag="ACHBB7_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM9210045.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1567..2427)
                     /gene="rlmB"
                     /locus_tag="ACHBB7_002119"
     CDS             complement(1567..2427)
                     /gene="rlmB"
                     /locus_tag="ACHBB7_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM9210046.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2420..2815)
                     /locus_tag="ACHBB7_002120"
     CDS             complement(2420..2815)
                     /locus_tag="ACHBB7_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM9210047.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2812..4224)
                     /gene="cysS"
                     /locus_tag="ACHBB7_002121"
     CDS             complement(2812..4224)
                     /gene="cysS"
                     /locus_tag="ACHBB7_002121"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM9210048.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4221..4757)
                     /gene="epsC"
                     /locus_tag="ACHBB7_002122"
     CDS             complement(4221..4757)
                     /gene="epsC"
                     /locus_tag="ACHBB7_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM9210049.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4843..5082)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5079..6539)
                     /gene="gltX"
                     /locus_tag="ACHBB7_002123"
     CDS             complement(5079..6539)
                     /gene="gltX"
                     /locus_tag="ACHBB7_002123"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM9210050.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6709..7866)
                     /locus_tag="ACHBB7_002124"
     CDS             complement(6709..7866)
                     /locus_tag="ACHBB7_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM9210051.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7900..9270)
                     /gene="radA"
                     /locus_tag="ACHBB7_002125"
     CDS             complement(7900..9270)
                     /gene="radA"
                     /locus_tag="ACHBB7_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM9210052.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9333..9860)
                     /locus_tag="ACHBB7_002126"
     CDS             complement(9333..9860)
                     /locus_tag="ACHBB7_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM9210053.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2507 a         2213 c         1784 g         3366 t
ORIGIN      
        1 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
       61 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
      121 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
      181 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
      241 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
      301 taaaattttc aagtttacca taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc
      361 attaagattt ttctaaatta ttcctcatta taaaatttca agtgtttttt tattttgagt
      421 tttactctcc cgtaagcgct cattacttgc ttgtgatcca ttgctaaaca ttcagccatg
      481 tctacaaaat caacgccttg catataataa tgaaaaattt tagcttctat ttttgaaaac
      541 tggatttcgc aattttcgat tgcttctttt aataaaagat gctgaatcgc taaaggttct
      601 tgcaatgtga tctctgctaa aaagtctggt ccttctgtct ctattttttg gtcgattgag
      661 acttcatctt tttgatcacg acgcttcagc gcgttctgct tcctcaacag agaatagatt
      721 tgattgctta taatgctttt ataataactt ccaaagctaa cacctttgct tttttcgtaa
      781 gttctcaatg cctgatataa agcgatcctt gcttcttgtt cccaatcctc agaaacataa
      841 tttcttaaat aataggtgtt tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg
      901 gcagcaaatg cttcttcgtc accattacgt gcagcgcgca ataactcctc agacataaaa
      961 taattcctcc ataaattcgt ttatggagga atcttatcat caatgattca tcgaactgat
     1021 tagaacgata tgttatttat tttttctcat taaatcgtcc aatttttggg acaatttttt
     1081 taactgctct tcattccatg gagaattacg tcggaaatca ataaaatgca tatctttatt
     1141 ttcttgggct attgtttttt ccgttttttt tatcgtctta tataattcat aagcagaagt
     1201 tcgtaatgct ccttgggaaa agatcatcca ctgttctgct aaatcgcttg tcgccactgt
     1261 cacttgtgtc aaacggtcat tcagctgacc agcgattctc tcgatataac tgtcagccgt
     1321 ttcgtcttct ttcgtaaaaa caacatccaa ctggtatttc ttataatttt gttggatgcc
     1381 cggaactagt tgggcatcga agacaacgat gacttccatt ccttcatatt tcgcataatt
     1441 tgataaacga tgtaaaagag cttctcttgc atccgccatt ttgtcctgct ttttaagctg
     1501 gacaagttcc ggccacgaac caatcatgtt gtagccgtct acaatcaaca gctgtttttt
     1561 catggtctac tccccctgac gttgggaaaa agcctgatac ataagcaaac cagcagcaac
     1621 actggcattt aagctctgga cgtgcccggt catcgggatc gtcagcaatt catccatttc
     1681 ttttttcaat cctggactca ttcctttgcc ttcattacca atgactaaag caattgatcc
     1741 tttggcattc catttttgga agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc
     1801 tgtttctttg agctgttgca cagcttgtac caaattcgtc acgcgtgcaa cagggacatg
     1861 ttcgactgcc ccagtggaag tctttgttac gataggtgtg attccaactg ctcggtgttt
     1921 tggtatgatc acaccatcga cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt
     1981 gtgtggatct tcgatgctat ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt
     2041 tcgttcaatc agttggtcaa gggataaata ttcataaggt gtaatcgcaa gaacgactcc
     2101 ttgatgcacg ccgtgatcgc tcaaagtttc taatttttgt ttaggaaccc atttgactgg
     2161 cacagcttgt tcacgtgcga tttctttcag ttcttctact ttttctcctc gtgaatcttc
     2221 ttgaagaaag agtttattcc ctcgcccttg ttgtagcgct tctactgcgg catggtgccc
     2281 aaagacgaaa ttatcttcta atgcttcttc attttgatca gaaactgact gatttcgtcc
     2341 agtttgcttt ttatttgaat atctattttg ttttttgtct tcaaatccac gtttcgattg
     2401 atgtttgttt gttctttctt tacccatttt tttcaccaac tttctgaata caccagcgga
     2461 tcaattcttc catccgctcg gtttgttttg tcaaatgcag atagcccatg agcgcttcga
     2521 accctgttgc gacccgatac gtcgtaacat cagcattttt tgcgctcgta tggcttttag
     2581 catttcttcc tctgcgataa taaagttctt cttcttctgt caaaagatgt tcttccagca
     2641 tttgctgaat caaaaaggct tgtgcattcg cagaaacata attcgttgcc gcacgatgca
     2701 gcttattggg ttttgtttct ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa
     2761 tcgcatctcc aacataagcc aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc
     2821 tcttctccat cgggttcctt gcggtgtatc ttctaataag atcccttttt ctttcaacaa
     2881 atcacgaatt tcgtcgcttc ggacaaaatc acgattgctt cttgcttgat ttcgttcttc
     2941 gatcaattgt tcaatctctt catctaataa ctcttctgct gtaaataaaa taccgaagat
     3001 cgaaagccat tgcgagaata aagcttctcc ttgtgccaag acagcggcag aaactttcgg
     3061 ctggtctaga taagtgttca gccacttagc aagctcatag accactgtga tcccatttgc
     3121 tgcattaaaa tcatcgtcca tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc
     3181 gattttatcc gcatcatctg ccaaattctc tgaggcagtt tcttggcgga atttcaaatt
     3241 gtcaaacgta ttcttcagtc gttgtaaatt ggtggctgct tctttcatcg tcgtttcact
     3301 gtagcggatc ggacgacggt attgtgtggt cgccatgaag aaacgcaaga cttgtggatc
     3361 aacgtttttg atcatttcat gtacagtgat gaaattccct agagatttac tcattttttc
     3421 atcgtcttcg ccaatcgtta catagccatt gtgcatccag taattagcga attttttccc
     3481 tgttttcgct tcactttgtg cgatttcatt ttcatgatga ggaaattcta aatcttgccc
     3541 gccaccatgg atatcgatcg tttctcctaa atgttttgtt gccataacgg aacattcaat
     3601 atgccaaccg ggacgtcctt tgccccatgg tgaatcccaa gaaatctcgc caggttttgc
     3661 ttgtttccat aaagcgaaat ctaatgggtc ttctttttgc tgctgttctt cccctgtacg
     3721 ctgacttgct ccgacttcca gttcatcgat ggattggtca cttaatttcc cataattttc
     3781 aaatttacgg gtacggtaat acacatcacc tgaagattca taagcatagc ctttttcaac
     3841 caacacttga ataaatgcta aaatatctgg catatgatcc atgactcttg gatgcagtgt
     3901 agcaggctcg acattcaacg ctttcgtatc ttcttcaaat gctcggatga aacgatcagc
     3961 aacttctggt gctgtaatac ctaattcgtt tgctgtacgg atgattttat cgtctacatc
     4021 cgtgaaattc gatacgaaat cgactttata gcctcgatat tcaaaatagc gtcgaattgt
     4081 atcgaaagca atggtgctac gagcatttcc gatatggata taattataca cagtcggtcc
     4141 gcagacatac ataccgacat gtcccttttg tatagggatg aattcttctt tttttctggt
     4201 caatgtattg tagattttta tcattctgat ctcctcactt tttctccttt gattcgaacg
     4261 acttttgctg gtactcctac cgctgtagcg tcgtctggaa catctttcaa cacaacggca
     4321 cctgcaccga ttttggcgtt ttttccgatc gttaccggtc ctaatatctg cgcattcgca
     4381 gagagcatgg ctccttgttt aacagttggg tgtcttttac ccgtttcttt tcctgtgccg
     4441 cctaatgtta cgccatggaa taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg
     4501 acgattccca ttccatggtc gatgaacacg ccagttccaa tcgttgctcc tggatggatc
     4561 tcaacaccag ttaggaaacg ccagaattga gcattgatct tcgccaataa aaagagccgg
     4621 tgctgatata aaaaatgaga gaaacgatgc caaaacaagg catgaaaacc agggtatgtc
     4681 aaaacgatct cagctgtcga acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct
     4741 cgggtaaacc aactcatgac tttccactcc tttcgtaaga attttatgta ggaaatcatc
     4801 ttcttataaa gaaaagaagc gccttttaaa taagtttcct tatcaaaaga cgctttccgc
     4861 gtggttccac ttttcttcat aatcaatgat tatctctgac agctaacgcc tgttatacgt
     4921 tcttggctac tttgtttcct ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc
     4981 actcccagag gcatttcggc aaccattcat tgataccttg caccacccgg tatctctctt
     5041 gtaatgatca gctgcttact tttttctgtt catcgtgttt agttcagtac ttgatttaaa
     5101 tgtgctaatg atttttcacg accaagcaat tcgatcgttt caggcaattc tggaccatgc
     5161 atttgtcctg atacagctac acggattggc atgaaaagat tttttccttt tacgccagtt
     5221 tctttttgaa cagctttgat ggcagctttg acagttggag catctactac ttccatttgt
     5281 tctagctgtg ctttgaatgc gtggagaaca gtaggaactg tttcaccagc aagtacttct
     5341 tttgctgctt cattcaatac aggatgttca ttgaaaaata gcgtagacag atcaacgatt
     5401 tctgcagcat agctcatttg aggttgatac aggctaacga tcttcttcac ccattcgatt
     5461 gtcttttcat ctgggttttc tgctacacgt ccatcagcga ttagataagg cagacacatg
     5521 tcagtaaatg catctagatc cattgctttg atgtaatggt tgtttaccca ttgcagtttt
     5581 ttcgcatcga aagctgcggg tgatttactt aggcgttttg gatcgaacat tttgatcaat
     5641 tcttggtgtt cgaagatttc gtcttcgccc acaggtgacc agcccaataa tgcaataaag
     5701 ttgaacattg catctggtag atagcctaat tcgcgatatt gttcgataaa ttgcaagatt
     5761 gattcgtcac gtttgcttag ttttttacct gtttcactgt tgatgatcaa tgtcatatgt
     5821 ccgaaggttg gtggtgtcca gccaaatgct tcatagatca tcagttgttt tggtgtattg
     5881 gcaatatggt catctccacg taaaacgtga gtgattttca tcaaatgatc atcgaccgca
     5941 acagcaaaat tataagttgg cataccatcg cgtttttgta tgacaaagtc gccaccgata
     6001 ttatcggatt cgaacgaaat agcgcctttc accatatctg taaatgcgta ttctgtattg
     6061 cgaggcacac ggaaacgtac aacagattct agaccttgtg cttctttttc tgcttgttct
     6121 tcaggtgtca agttcgcaca tgttcctgcg taatgaggca tttctcctct tgcgcgttgt
     6181 gcttcccgtt ctgcttctaa ttcttcttct gtgcaatagc atttgtatgc tcgattgcta
     6241 gcaagcagct gatcaatcag cggttgatag atttctttac gttcagattg acggtaaggg
     6301 ccgtattctc caggattttc aggagattca tcccaattca ttcccaacca agcaaggttt
     6361 tccaactggc ttttttcgcc atcttcgata ttacgttttt ggtcagtatc ctcaatacga
     6421 ataataaagt ctccatcatt gtggcgggca aacaaataat tgaataatgc agttcttgca
     6481 tttccaatat gcaggtgccc agttggactt ggtgcatagc gtacgcgtac tttcgtcatt
     6541 ttcttcctct tctttcaaac tagactttga ctaacagcaa aaattccgtc agttacggct
     6601 tctcaaatat catttattta aagccactca gcacaaattt taccttttct aaggtaaata
     6661 gtaaagccaa ctggttgaat ttccttacca gttggctgaa tttctttttt attttgattg
     6721 tttagtcttt ttctctgtct cttttttatt gtcgattcct cgtcctgcat gtgcaggttt
     6781 agcaaagatc atgcgtccag cagctgtttg aagggcactt gttacaacga cttcgatacg
     6841 ttcattcatg taatgctgtc catcttctac gacaaccatc gtgccgtcat caaggtaggc
     6901 tactccttgt tggcgttctg tcccggcttt tactactaga acattcatcg tttcaccagg
     6961 gatgacgact ggttttacgg catttgccaa cgcattgata ttaagaacag gtacattttg
     7021 gaattcagag actttattca agttatagtc attggttaca atgacaccat ctaaaagctt
     7081 agccaatttg atcaatttgc tgtctacttc actgatatct tcaaagtctc catcgtacat
     7141 ttcgacagat ataccctctt ctttttgcaa ggcatttagg atatctaatc cgcgacgacc
     7201 acgcacacgt ttcaaactat cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa
     7261 attagggatc atcaaggttc cttctaagaa tccagtcttt gtaatatcat aaatccgacc
     7321 gtcaatgatc acgcttgtat ccaagatttt atatttatgg aaattttctc ctgattttcg
     7381 ttctaacagt tgactctcaa cttgactagc tgtttcttgc tcgatttttt tgcttcgtga
     7441 accaaaaact ttcttccatt cttcgattcg tgtcgttccc attctcaatc ccagatagcc
     7501 taagatgatc atcagtaaaa tcggcaatac actattgacg aatggaattt ccatattgta
     7561 aagaggggtc gagatcacaa cacctaaagt caatccgatg atcgcaccga tcgcaccgaa
     7621 gagaagataa gtcaagctga cctcacttag tttttgttct acttgtttga taatagctga
     7681 gatatgtttg gccaacagca aagataataa aaagaaaata agtgcgccta acaggctgtt
     7741 ggtaaatgta ttattcagcc atccgtttga ctgttgaccg attgcctgcc aagctgaggg
     7801 taaaaatgaa atacccaagc ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt
     7861 ttgcatcgtt tttcctccgt ttcgacaatc atttattagt tagcggaaca cacgcttcaa
     7921 cgtttcagcc aaagttgcta ctccaactat ctcgattcct tttggcgctt cccagttacc
     7981 taagttgttt ttaggtaggt atacttttgt gaagccgagt ttttggactt ctttgactcg
     8041 ttgttcaata tgactgacac gtctgatttc tccagtcagt ccaatttcac cgatgaaaca
     8101 ttccgttggc tgtgttcctt tttctttata gcttgaagca atactgacag cgatagctaa
     8161 atcaatcgct ggttcattta attttacccc acctgcagct tttaagtaag catcttgatt
     8221 ctgaagaagc aacccagctc ttttttccag cactgccatg atcagagata cccgattgaa
     8281 atcaagacct gttgttgttc gtttggcatt tccgaacata gtaggtgtga ctaatgcttg
     8341 gatctctacc aaaattggac gtgtcccttc cattgccaca acgatagcag agccagttgc
     8401 accgtctagg cgttcttcta agaaaatctg tgaaggatta gcgacttctt ccaatccatg
     8461 ctcatgcatt tcaaaaatcc caatctcatt cgttgagcca aatcggtttt ttactgctcg
     8521 caaaatccga aaactatgat gttgttcacc ttcaaaatat aacaccgtat ccaccatatg
     8581 ttctaacatt cttgggccgg cgatcgagcc ttctttggta acatgaccga cgataaaaat
     8641 ggctatccca tttgttttag cgattttgag tagttctgca gtggtttctc ttacttggct
     8701 gacacttcct gccacacttg tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat
     8761 aacataatca ggggagatat gttcgatggc acgactgatt tcgttcatat ctgtttcagc
     8821 gtatagataa aattctgtgt tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc
     8881 tgcgctttct tcgccggata catataagac ttttccgcct atggctgcta attgctgaga
     8941 aacttgtaac agcaaagtgg atttcccgat acctggatcg ccaccaatca agaccataga
     9001 ccctggcacc acacctccgc ctaacacacg gttcagttct tctaattttg ttttgatccg
     9061 aggttctttt ttagggacga cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa
     9121 acttgtccga gttctgcggt ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca
     9181 ttttccgcaa tttgggcagc gtcccaagaa cttaggcgac acatacccgc aattttggca
     9241 aacaaattgt acttttgatt tttttgccat tgtaccgcct ccttttatcc taatcatgag
     9301 acattaaatc aagcttaagt atctttaacg ttttatttac tggaagaacc aaatccgcct
     9361 tgacgttgag cagtcgtttg atcttgatct gcttttaaaa acggcaaaaa gatcccttgg
     9421 ccgatacgct cgcctttttt gatcactaca tccttgaaac caaagttggt gaattggaac
     9481 atgatatggc cttcatttgc ctcattattg taataatcac tgtcaatcac acctactccg
     9541 tttgtcaaca ctaaaaaccg cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat
     9601 tcatcttctc ccatgtaagc tttgattcca gtaggcacga gggttggttt caattgtttt
     9661 tcattttctg atacatcttt tttcaatcca tctgcgactg tttccaaccc ctttttccag
     9721 cctgctttcc agatacttgg aatcacgacc tcttctgctg cttcaaaatc atagccagca
     9781 gcatgatgtg tagcgcgctg cggtagattg atattttgat ctttatattt agaaataact
     9841 tcaaatcctc tttttctcac cggttttcct
//
LOCUS       DAYYKN010000100         9602 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000100 DAYYKN010000000
VERSION     DAYYKN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.101"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACHBB7_002127"
     CDS             complement(<1..613)
                     /locus_tag="ACHBB7_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFM9210054.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            814..2028
                     /locus_tag="ACHBB7_002128"
                     /pseudo
     CDS             814..2028
                     /locus_tag="ACHBB7_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2207..2542)
                     /locus_tag="ACHBB7_002129"
     CDS             complement(2207..2542)
                     /locus_tag="ACHBB7_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9210055.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(2526..2789)
                     /locus_tag="ACHBB7_002130"
     CDS             complement(2526..2789)
                     /locus_tag="ACHBB7_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM9210056.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(3249..4052)
                     /locus_tag="ACHBB7_002131"
     CDS             complement(3249..4052)
                     /locus_tag="ACHBB7_002131"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9210057.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(4030..5154)
                     /locus_tag="ACHBB7_002132"
     CDS             complement(4030..5154)
                     /locus_tag="ACHBB7_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9210058.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(5170..5985)
                     /locus_tag="ACHBB7_002133"
     CDS             complement(5170..5985)
                     /locus_tag="ACHBB7_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9210059.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5975..6847)
                     /locus_tag="ACHBB7_002134"
     CDS             complement(5975..6847)
                     /locus_tag="ACHBB7_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9210060.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6932..8221)
                     /locus_tag="ACHBB7_002135"
     CDS             complement(6932..8221)
                     /locus_tag="ACHBB7_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9210061.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(8365..8898)
                     /locus_tag="ACHBB7_002136"
     CDS             complement(8365..8898)
                     /locus_tag="ACHBB7_002136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210062.1"
                     /translation="MANEKATMTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQL
                     SWAAIWGKHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAR
                     EVGQQLGLTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGV
                     KRFGDGIHDHMGALFLA"
     gene            complement(8944..9087)
                     /locus_tag="ACHBB7_002137"
     CDS             complement(8944..9087)
                     /locus_tag="ACHBB7_002137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM9210063.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2967 a         2027 c         1554 g         3054 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccac caacacaaaa tattatagaa gcaaaatcaa ttctacctta gatgttctta
      781 tatcactcta tcagtcctat ttgacaaaga gccaagaaaa agacgttggt cgtcgatgct
      841 gttctctcgc ctactcctcg tgcttgtaga agttgtggtt ctactgtggt agatggaaac
      901 gggaaagcaa ttatagtgaa aaatgggaag aaggaaacga ttgtccgttt tgaacaatac
      961 aatcatatgc ctttggttac gcgcctaaaa aagcaacgct atacctgtaa aaactgtcgt
     1021 acccattgga cggctcaaag ttattttgtc cgactcagcc attcaatcgc agatcatgtt
     1081 cgatataaaa ttgcttcttt actgactgaa aaagtatctt tgtcttttat tgcgaaaagt
     1141 tgtcaggtat ctttgactac cgttactcgt acattgaaag agtttaaaag ctatttacca
     1201 aagccttcta agaagattct ccccagagta ttgatggttg atgaatttcg ttcgcacgct
     1261 tccatagaag ataaaatgag ctttatttgc gcagatggtg aaacaggaaa cttaattgct
     1321 gtcttgccta cgcgtaaatt acctcgatta acaagctatt tcttaggctg caccaatcca
     1381 gaagaagtag aattcttagt cacagacatg aacgccgctt acttccagct taccaaacgt
     1441 gttctgccaa atgcgaaagt agtgattgat cggtttcata ttgtcaaaca catgaatcag
     1501 gcactcaatg agttgcgtat ccgtgaaatg aatgagcttc gtaaagcagg acagaaaagt
     1561 caggcagaaa aactgaaaaa gaactggcgc tttctgctaa aaaatcgtgc aagcatcaac
     1621 cattatgagt acaaaacatg gaaaagtttc cgagcaccaa aatacccatt tctcactgaa
     1681 gcaatgatga ttgatcgatt gcttgagttt tctgcgcctc taaaggaggc gtatcccttt
     1741 tttcatgaat tagtcgaagc ctttcgagac aaagaccctg acttattttt ctccttattg
     1801 gcagaacttc ccgaaacgtt agatgacggc tttcgggaga agcttcaaaa ccttctgacc
     1861 tatgaagaag gtatcaccag cgcaatgatc tatccttatt ccaatggaaa aattgaagcg
     1921 aagaataccc acataaagac aatgaaacga gtatcctacg gatttaaatc attcgagaac
     1981 atgagaatta gaatcttttt gatcaatcaa ttaatcaatg taagataaca aaaaatctga
     2041 accagaatgt ggttcattct gacccggatt tgattttgca caactcatca gtccttattg
     2101 acaaagagcc aaaaatctga gccagaatga ggttcattct gacccagatt tgattttgta
     2161 caacccatca gtccttattg acaaagagcc cttcttttta gtattattat aattcttcca
     2221 tgcgttcttc cagtcgctgt aaatcgcttg gacttaattc cccaattttt ttccatttga
     2281 cctttttgtt gtttttgggt aattcgttta gagttcctac gtcaatccaa gattcttttt
     2341 ttagtcctgc ctgtttccaa tcccttattt caacgtattt cagctttatt ttatcggatt
     2401 tagtatgata ttttgaagta attgacagaa acgaaattgt gtccagtcca ttttttagaa
     2461 tatagacagg ccggcgttta ccgccctctg cagctgtgaa agcaacgtag acggttgcta
     2521 tatcattagt attcatcttc attgaaccat tcttcaattt tttcgtcagt gtctagtttt
     2581 tggacaggca atttttccgc taatctttga atattatgga ttcgtttact tttttccgac
     2641 aattcaatag aaaatgggat ttttccttgc tcaacaattt gcttataaaa aacggtgacg
     2701 gctgttttgg ggctcatgcc tagttcatca ataatgcttt ctgcttcatt tttcaatttt
     2761 tcgtccatag aaatttgtaa cttagccatt ttctccacct ctttctgaca aacacattat
     2821 atcacggaaa gagttcaaat ggaattaata caactacttt tgaactcctt gaaatgcagt
     2881 gtatttatct aaatataagg aaaggatata gtgctaaatg gccttgatag atagaaaagt
     2941 ttcaaaatcc gccacagcga taattgaagc gaacactcaa cgacacacag aacaggaaaa
     3001 gactcctaaa agaaccaaat taactaggac gtgtctgaaa actccacaaa gatgtggtat
     3061 gctctggtaa aaaacgaatg gcacaaccac gaagaccgat atggttcttg gaaaactttc
     3121 tccacccttt ttgtaattgg agggatagtg gtttattgat agcgattttc caagttcttc
     3181 aagtggaacc agacattgag caccacggct aaaaagtgaa tgaagtcttt atttttaatc
     3241 aataattttt aatgattacc agtaattggt tttttcatgc gccctatttc tatttgatgt
     3301 ggagttaaat aatgctgata agtaaaaatt tttccgtcaa ttaaactgcc actagtgaat
     3361 cctgttctat tggtaaaaag agaatagtct gggtattcat ctacaccaaa tgataaagat
     3421 tcagccataa actgtttttt accagcaaat tcagcactgc taccttgatg gatatgtcct
     3481 acaaaaatac ctaaaatatc actttcggca atcaattcag caaaaccttc cggaacttca
     3541 gttacaatag atggatcttc ccacgacaat ggatgatgaa ccaataaaat cgttcccttt
     3601 tcagcaggtg tacccaacca ctcttgtaat tgaatcatct gtcctatcga tatttttcct
     3661 tcgtgctggg tatcttgagc agaatccaag gaaataattc gtaacccatt ttgattcgta
     3721 caatggatat actctcccgt ttcattgctt tcaacactca ttccttcaaa gaaaacactg
     3781 cgacgatcat gattcccacg acaaaaatag taaggtgtgg ttgggaaata ttgatccaac
     3841 attttccgta agacttgata atcagaaatc cccccttcat gtactaaatc acctgttaat
     3901 agaataaaat cgtacactgt taacgagagt tcttgtaaca atttttccaa tgccaactgt
     3961 ggatttttaa ttgttggaaa tagttcagca gtaaacaagt tagtttgata gttttctcta
     4021 aaatggatat cagagagatg caaaaagcgc atggctattc cctctttctt caataaaata
     4081 atcaggatat cctaaatttt cttgcgtctg ataatcaaaa aaatgcattc tttcaggttc
     4141 tatgcggaga ttgactgcat caccaatctg ccaagtaggt ttttcattaa ggacaatcat
     4201 ttctatacca tcgactttaa cggtgtacgc ataactttga cctaatagct cacgatactt
     4261 cacttgaccg tgtaaatcgc ttgtttcttt tgaaaaggtt acgtgttctg gtcttatgcc
     4321 taaatacaat gagccactaa cctgaagaag ttgtttttgc caagctggat tcaatgttaa
     4381 aaattgactg gctaccttta atgttccttg atcataagta actggcataa tattcatgcc
     4441 tggcgaacca ataaaatttg cagtaaaagt atttttaggt ttcgtataaa catttttcgg
     4501 cgtatcaatc atctgaattc taccttcatt caataaaaca atccggtcag ctaacgtcat
     4561 cgcttcaatt tgatcatgag tgacgtagac aaaagtttgt ttataacgtt gatgaatttt
     4621 taccaattct tttcgtgcat ccaatcgtaa acgcacatcc aaatttgata acggttcatc
     4681 taataaaaaa taattactcc gtttaaccac cgcacgagcc aatgctacac gctgccgttg
     4741 ccctcctgac aaatctttag gaagtcgatc ttggtatggc gttaaaccta acatcttcaa
     4801 agcttcttcc aaacggtgct gaatgtcagc tgtcttaatt ttattcgccc ttaatgcaaa
     4861 aataatattc tcctttacag tcatgtgagg atataaagca taattttgaa aaaccattgc
     4921 gatctctcgt tctccacaag gtagcttatt tgctagttgt gtacctaaaa aaagctctcc
     4981 actcgtaatt ttttccaaac cagcaatcat tctcagcaaa gtagattttc ctgaaccaga
     5041 tggtcccagt aacacaattc gttctccagc ggccacagct aaatcaattt cttttaaaat
     5101 ggtatgttct ccatattttt ttccgatatt tcttaattca agcgtttctc ccatgatttg
     5161 tcctccaatt tatttaatac ctgcagaaac aaaagtcccg ataatatatt tttgaaaaat
     5221 tgcatacaag actaacggta caaacattgt aattaaacta atcgccattg ctacggtaaa
     5281 atttgtccca ccttcagaac taatataggt ctgcatcgcc aacggcaaag tataattatc
     5341 tactgtccgt aaaattaata ccggccaaac atactcattc caagaggtaa taaaaaacca
     5401 aataccggta gaagtaatcg catgtctagt aagaggaaaa acaattttac tcatgacggt
     5461 tgtttgtttg atgttatcca atgccgccac atctagaagg ctcactggaa tttcccgcat
     5521 tgtttgcgtc aataaaaaaa tgcccatcgc atcagcaaat tgtggtatta tcacacccca
     5581 agtagtatca attaaattaa gttttgcaat caacaaataa ttaggaatca tcgcaacagt
     5641 aaaaggaatg aacatcgtac caatgatgat aaagtaccag attttcttcc ctttgattcg
     5701 gcaataagta aaactatatg ccgcaaaaaa agctacgcct aacttaacaa ccgttataat
     5761 tgtagcaata aaaaaagtat ttctcgtaat tttgataatt ggaagatttt cgaacaaata
     5821 gcaaaaattt tccaaggtaa acggctgtgg tatcaaacta gtaatcgtct gataggcatc
     5881 tttcaatggc ttaaaagaat tggccataat aaacaataat ggatagataa taatgattat
     5941 gatgaacata aataagagat gtggcaacca gcgtttaatt ttcatagtaa accccctttt
     6001 ccacaaattt aaatattacc cctaagaaaa cagcaaatat taataaagtt aaaactgaaa
     6061 ttgctgaaga ttctccgact ttatacattt caaaagcctt ttgataagca taatagatca
     6121 cgtttgcact accattattc ggtccaccac gagtgatgat cttaatcgga gtaaacacat
     6181 actgcagacc ttgaacaatg gtcatcacca ataaatatag tcctgtcgaa ctactcatag
     6241 gcaacacaat tgtaaaaaaa atgcgccaga tgggtacacg atccaactta gctgcttcga
     6301 tcatctcttg atcaattccc acaattgctg caaaaatgac aatgaaatta taaccaaaaa
     6361 ctttccaagc agtaatacct gcaatcacag caattaccca accttgtaaa ttcatccaca
     6421 taggcagtct taaaccaaaa tttcccaaca agacagcaat tggtcccgat accggattta
     6481 atatccatgt aaaaaccatc cccgccacta ccaaagaaat cacagctgga ataaaaaata
     6541 atggtttata caaattctgc cctttttcta taactattcc taataaaaag gatgtaatat
     6601 atgggaacac acagctcagc aagacaaata gaacggtata ggacaaagta ttcttcaaca
     6661 ctttatgaaa gactggatca gtcagcacct ttacatagtt ggcaaagcca acaaattctt
     6721 tagtgggggc caccatattc cagtcaaaaa aactcaaata gatcgtataa atcaatggat
     6781 aaaaaacaaa cacgcccaac aaaataattg ctggcgccag atacaaataa cctacatagc
     6841 gcttcatttc cgacagcctc ctcatcctta tgtcaaaagc agacgacctt gtttttcctt
     6901 tcttacaggc gctgtcgtct gcttgctttg cttatttgag taactcatta atttcctttt
     6961 gagtagcttt catcgtttca ttgacggaat tacctgataa aattttatcc cgcatatcca
     7021 acaatttttg ttcagcttct aatccagcat ctccaggaaa tgaactccaa ctgaccatct
     7081 tatccatctg atcaattgct ggtatcatca tctgattttc agctaagaaa gcttttaatc
     7141 cattttgggc gtctgcaaca tctttacgtg gtggtaaata accagtacct tctgtccatt
     7201 tagctaaaga ttcaacacta taaagatatt ttaagaaatc ccaagttgct tcctttttct
     7261 tatccgtttg tgatgtaaca gctaacattg cgccaccagc aggtagttta acttttttcc
     7321 cttcccaact tggtgactca actgctttag cagtaaaagt actatttttt tgaatgttgt
     7381 ccctttgagc aatagtggta tagaccatcg cgacgttacc atcaataaag ctttgcacac
     7441 cttgttccca cgtagtgtgt aatgccgtct ggtcttttac catcctttga tataattcat
     7501 aagcagcgat accatcttta gaagcaaagg tagctttgcc atcttttatt acacttgccc
     7561 cattactttc taaaattgct tgttgtgccc aattatcagc aggttcttga atatacacgc
     7621 catactttcc agttttttct tttattatct tagaaaaagt ttgaacttct tcccaagttt
     7681 ttggaccatt ttcatccaag cccgctgctt ttaatatatc ttgatttaaa tataaaaccg
     7741 gattactaat ggagtaagga atacccactt gtttaccatc tttttcagac agtgccatga
     7801 tattgtctaa aaaatgatct ttaaaataat cagcattatc cggatccact tcttctacta
     7861 aagattgagg attagtatat ttgaaatttt ctgcaaaata atctttaaaa gcccatccca
     7921 cttgtactac gtccggtatt tctttggagg caacagaagc ttgtaaattt tgcatcaagc
     7981 ctttatacat atcaggatta tattttgcta caaccttcac tttgtcttga gattgattgt
     8041 atttatctac taattcattc actgtttttc cacctagggt atccgcatta acatgccaat
     8101 actctatcgt tacacgaccc tccgattttt ttgttgtctt ggtattccca ctaccacaag
     8161 ctgccaaaga gatgacactt aaaacagaca caacccctat aacaagattt ttctttaaca
     8221 taacattaac ttcctcctaa tttctttatg gccatttttc taatcaagaa atattatgaa
     8281 ctaccaatgt aaatttctag ttaattttct gtaaataatt cgaaatttgt gcatttcggg
     8341 gtacagggtc actggacata tccatcaagc taagaataaa gcccccatat ggtcatgtat
     8401 cccatctcca aatctcttaa ctcctctgtt ttgattataa aatagtttat tctgtgctcc
     8461 tttcttccat aaataggccc atgcacaacg tgaggttata ttataaacac cacagcgcgc
     8521 aaggtactca atcgtttcta gttcattgac tgtccataca gaaaatgtca atcctagctg
     8581 ctgccccact tcacgggcca acttagttaa ccatccttca tgaatattga cgatttgaac
     8641 gctttgatag gctaatttct gtaaggtatc gatcaaatct gcttcggtaa tatccccaag
     8701 ttttttccct tcgaactgat tttcaatatt ataaaatata tgctttcccc aaatcgcagc
     8761 ccaagataat tgtacatagc cactaagaac aatttgtggc cagatttgat aggtttgtgc
     8821 cagcgaaaaa acggccattt ccaatccctc ttcttttaaa tcacagttaa tggtcatggt
     8881 tgccttctca ttggccactg cttgaaacac ctgctcaagg gttaccgaat ctgggacaac
     8941 ttcttacctg tcatgggcta gtattagcgt accatctgtt tgctgacgca catctacttc
     9001 aaaacaaagc tggcgatcca aacaaaagcg aagaaatgcc cagctattct cctccgtatt
     9061 gtcactacca gagtgtgcag tgatcatggt cattttgacc cctcctttaa ttccgtgatt
     9121 ttctcccaca cgtccactat accgatgaag cgttaatttc ttgttaattt cttgtaaatt
     9181 ccctgcgttt tttcagtaaa ctagttgtaa atcttgcatg ctccttccta cgaactcatg
     9241 aatcgtcgat tttgcccttc tagctagttt caccaattaa gtagttctac attccccgct
     9301 tttaacctac tacacctttt tttgttttca gctattcctt cttcttgctg aaagtccttg
     9361 aggttctttc ttacaggata ccatcaaggt gttcaatggc ttattcccat tgaatgaccg
     9421 agacttacta tcagatttct gctcgttgaa ccaagtcttt gatacaggat ttcttccgtc
     9481 atcttctcac aaataccacc ttgcgtttct ctagcgattg gtcgatgtgc accctcccag
     9541 tggactgtca ctaccccttt ttgctatgcc tagcccacaa aaaaagctgt cctcacagac
     9601 ag
//
LOCUS       DAYYKN010000101         9551 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000101 DAYYKN010000000
VERSION     DAYYKN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.102"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..417
                     /locus_tag="ACHBB7_002138"
                     /pseudo
     CDS             1..417
                     /locus_tag="ACHBB7_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(421..1140)
                     /locus_tag="ACHBB7_002139"
                     /pseudo
     CDS             complement(421..1140)
                     /locus_tag="ACHBB7_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(1091..1300)
                     /locus_tag="ACHBB7_002140"
     CDS             complement(1091..1300)
                     /locus_tag="ACHBB7_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210064.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(1282..1350)
                     /locus_tag="ACHBB7_002141"
                     /pseudo
     CDS             complement(1282..1350)
                     /locus_tag="ACHBB7_002141"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            1577..2850
                     /locus_tag="ACHBB7_002142"
                     /pseudo
     CDS             1577..2850
                     /locus_tag="ACHBB7_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2957..3988)
                     /locus_tag="ACHBB7_002143"
     CDS             complement(2957..3988)
                     /locus_tag="ACHBB7_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9210065.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3995..4825)
                     /locus_tag="ACHBB7_002144"
     CDS             complement(3995..4825)
                     /locus_tag="ACHBB7_002144"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9210066.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4822..5637)
                     /locus_tag="ACHBB7_002145"
     CDS             complement(4822..5637)
                     /locus_tag="ACHBB7_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9210067.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5634..6458)
                     /locus_tag="ACHBB7_002146"
     CDS             complement(5634..6458)
                     /locus_tag="ACHBB7_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFM9210068.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6448..7548)
                     /locus_tag="ACHBB7_002147"
     CDS             complement(6448..7548)
                     /locus_tag="ACHBB7_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9210069.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7726..8511
                     /locus_tag="ACHBB7_002148"
     CDS             7726..8511
                     /locus_tag="ACHBB7_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM9210070.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8544..8927
                     /locus_tag="ACHBB7_002149"
     CDS             8544..8927
                     /locus_tag="ACHBB7_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM9210071.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9003..9134
                     /locus_tag="ACHBB7_002150"
     CDS             9003..9134
                     /locus_tag="ACHBB7_002150"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9210072.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9103..9339
                     /locus_tag="ACHBB7_002151"
     CDS             9103..9339
                     /locus_tag="ACHBB7_002151"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9210073.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            9351..9437
                     /locus_tag="ACHBB7_002152"
                     /pseudo
     CDS             9351..9437
                     /locus_tag="ACHBB7_002152"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(9417..>9551)
                     /locus_tag="ACHBB7_002153"
     CDS             complement(9417..>9551)
                     /locus_tag="ACHBB7_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM9210074.1"
                     /translation="IELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF
                     "
BASE COUNT         2796 a         2267 c         1850 g         2638 t
ORIGIN      
        1 gttccatgag ttttatttgt gttgatggga caacccatca gctatttacg atccttgaag
       61 atcgcagact ttacaaacta actcaatact tcctgcactt tcctagaaaa gcccgtttaa
      121 aggtcaagta cctagaatgc cagctattgt cagctgctta aaacggtctt tccaaatgcg
      181 gagatcgtaa ctgatcgctt tcatattgtc caacatatca atcgcagctt caatcaattg
      241 cgtatccaga tcatgaatcg ttttcgagcc tcgcactctg aaaatcagaa gaagtaccgt
      301 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccgctcaaa
      361 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt cgatgaaccg
      421 ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag cagtcgccac
      481 atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat gttcaggatc
      541 ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac
      601 cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt aatcgattgc
      661 tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc atgaactatc
      721 gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca tttgttctaa
      781 caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt tatgcccgct
      841 aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg
      901 ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc gtttcaagca
      961 caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga
     1021 atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc
     1081 agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat
     1141 ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct gctgccgtcc
     1201 caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg
     1261 cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa tcatcgtccc
     1321 atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa aacaagcaaa
     1381 agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa aagtcaaagg
     1441 tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta tcagtccttt
     1501 tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat
     1561 caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag gaatcaaaga
     1621 tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca acagtatcgt
     1681 tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac aggtgaatca
     1741 acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca ataagtgtgc
     1801 gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac
     1861 ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca aacaagtaca
     1921 ttataaaata ttggaggaat tgactgaaaa acaagcaatg acgacgattt ctaaacattg
     1981 cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca tgacaaacgt
     2041 gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca
     2101 agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac tggatattct
     2161 tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac gacgtgcacg
     2221 tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc tgataaaaga
     2281 gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc acctcattcg
     2341 cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg atccaataca
     2401 agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac gacaggacac
     2461 gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt taaccgaaag
     2521 tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt atgcttacta
     2581 tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc agcttgtaaa
     2641 aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt ttataaacta
     2701 tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa tagaaaatct
     2761 gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag aaccagaatt
     2821 ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag cagaaaatca
     2881 attctacctt agatgttctt atatcactct atcagtccta tttgacaaag agccagaaat
     2941 gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt tccacaggga
     3001 ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct tcccgatcgt
     3061 tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac cgttgaatgt
     3121 tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga ggaaccggtt
     3181 caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta gcggaaaact
     3241 cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg ataaattccg
     3301 caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc tgagccaccc
     3361 gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct tggtcatgag
     3421 tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc cgctgcagct
     3481 gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg actttcggtt
     3541 cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa agaaactgga
     3601 catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa accttttcct
     3661 gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt tcaaaaacag
     3721 tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga tccttcgtag
     3781 gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt tgttccaatc
     3841 ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt aaagtaacca
     3901 gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca
     3961 gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag ggcttctttt
     4021 tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt cgataagtca
     4081 gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc atcacagcgc
     4141 tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc
     4201 gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa aaaatcatca
     4261 gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata ataatcgtag
     4321 acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta attaagcgca
     4381 tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat gcacctacca
     4441 acactacaaa cattgggata ccagtttcag catacgttcg aagcaaagcc gttaccaaaa
     4501 tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga ttcaaacgag
     4561 ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc gtcaccaaga
     4621 ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg tccgtcagca
     4681 gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac catttagtgg
     4741 agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca aagcagaaca
     4801 aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt gaataatcaa
     4861 cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta aattaggctt
     4921 ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat ttacattact
     4981 gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata agatgacaaa
     5041 ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag catacgctga
     5101 tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct tcatgctggg
     5161 atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg tcaatcccca
     5221 ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca cacctacatt
     5281 ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag ctgccaaatt
     5341 ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc ataaacataa
     5401 aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta cgctgttaac
     5461 aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca atgtccactg
     5521 gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca gcagtaaaaa
     5581 tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct ctttcatccc
     5641 ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa taaccagcaa
     5701 aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg gcgtttattt
     5761 gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc
     5821 gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag ccattcagga
     5881 aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc atgagaatcg
     5941 gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa aaagtctggc
     6001 tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc
     6061 tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat gcgatcaacc
     6121 aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc ggcggctcct
     6181 gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga tcgctgagca
     6241 tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag tacttcgtat
     6301 aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc ttgctggtgc
     6361 tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg acagccgtct
     6421 agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca
     6481 aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc
     6541 cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca gctctacctt
     6601 gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag ataaaatata
     6661 ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta aggttgtata
     6721 cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg agtcaatttg
     6781 gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt cacctttttc
     6841 aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca tttttttgta
     6901 gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg ccgcagccaa
     6961 tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt gatagtcacc
     7021 ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat cggtgataaa
     7081 ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg cccaatccgg
     7141 aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt cagccaacgg
     7201 accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt ctgattcaaa
     7261 cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc cgtattcttt
     7321 ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc ccaagctggc
     7381 aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt ctgccgcagc
     7441 cgattgcttc gttgcttcac ctgagccgca gcctgtcagc aggatactta ctgttgctgc
     7501 acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc attcttctcc
     7561 cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg
     7621 tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc
     7681 ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca gtattatgat
     7741 cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc
     7801 tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc atctaccgga
     7861 aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt aaaagaaacc
     7921 tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca agaagagcgg
     7981 atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat tcatcaaaat
     8041 ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca aactaccaag
     8101 gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga tattttgact
     8161 cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc ggagcatttt
     8221 gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc gatggagtta
     8281 aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc cgttcctttg
     8341 tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt tgccgaagat
     8401 tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt agaaaagttg
     8461 ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg attcctatat
     8521 aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa gaggcaccca
     8581 tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt tgccgatcct
     8641 tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac agcttgcagc
     8701 gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa tatttgcatg
     8761 cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat ttacgaggac
     8821 agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg ggaatggctc
     8881 gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg caggatcaag
     8941 gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag gaagaagttt
     9001 gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac caagctcccc
     9061 tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac acacctttcg
     9121 gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc tggtgatgaa
     9181 gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact ttatcatttt
     9241 aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca gttttttatt
     9301 gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac ttggagcttg
     9361 gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac caagggttag
     9421 aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg attgagccag
     9481 tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac tgacttcagc
     9541 aaaagctcta t
//
LOCUS       DAYYKN010000102         9506 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000102 DAYYKN010000000
VERSION     DAYYKN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.103"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..219
                     /locus_tag="ACHBB7_002154"
     CDS             25..219
                     /locus_tag="ACHBB7_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210075.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            488..787
                     /locus_tag="ACHBB7_002155"
     CDS             488..787
                     /locus_tag="ACHBB7_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210076.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(884..1318)
                     /locus_tag="ACHBB7_002156"
     CDS             complement(884..1318)
                     /locus_tag="ACHBB7_002156"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9210077.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1550..2260)
                     /locus_tag="ACHBB7_002157"
     CDS             complement(1550..2260)
                     /locus_tag="ACHBB7_002157"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM9210078.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2250..3110)
                     /locus_tag="ACHBB7_002158"
     CDS             complement(2250..3110)
                     /locus_tag="ACHBB7_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM9210079.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3115..3753)
                     /locus_tag="ACHBB7_002159"
     CDS             complement(3115..3753)
                     /locus_tag="ACHBB7_002159"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM9210080.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3768..4067)
                     /locus_tag="ACHBB7_002160"
     CDS             complement(3768..4067)
                     /locus_tag="ACHBB7_002160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9210081.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4110..5594)
                     /locus_tag="ACHBB7_002161"
     CDS             complement(4110..5594)
                     /locus_tag="ACHBB7_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9210082.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5614..6075)
                     /locus_tag="ACHBB7_002162"
     CDS             complement(5614..6075)
                     /locus_tag="ACHBB7_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9210083.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6093..7160)
                     /gene="ulaG"
                     /locus_tag="ACHBB7_002163"
     CDS             complement(6093..7160)
                     /gene="ulaG"
                     /locus_tag="ACHBB7_002163"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM9210084.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7378..8148
                     /locus_tag="ACHBB7_002164"
     CDS             7378..8148
                     /locus_tag="ACHBB7_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9210085.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8421..9248)
                     /locus_tag="ACHBB7_002165"
     CDS             complement(8421..9248)
                     /locus_tag="ACHBB7_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210086.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2956 a         1945 c         1494 g         3111 t
ORIGIN      
        1 aacagcaaga aagaagagga tcaaatgtat tatgtagaag taaaaacaaa aggcgtcaaa
       61 aacaaacaat atgtgaaagg tatcagtaac gaatatcctt tacttggttc atggaaagaa
      121 gctgctcctt tttccaaacc atgtgccata aaaataaaga acgagctaga aaaagaactg
      181 acttgtggta aagctgttgt agatatcatc gaaaagtaaa tccaatgtat agaaaggaag
      241 atcacacatg aaaagcgacg tacaaattgc taagattata gaattagaag aagaagacgc
      301 agtgttgaca attgaaaaaa aataaatgtt tgtataccat atgattattt caatatgtat
      361 cctggaattg gagatctcgt agagctatat caagatgaat caaatatact tgtcattcat
      421 catgaaatat agagacttat aaaattgttt atttttataa tgtatcagta tagaaaggaa
      481 ataaaacatg gattatactg ttgaatttga cagtgaacta cttaagcaat ttccacaaga
      541 agaacatttc atgtataaag aagcaatttc ccacaaagtg acagagtatt tggagcataa
      601 tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa ggaatatacg agatgaaagt
      661 caaaattaat aaaaaaaatt atcgcatagc atttgcttta aaaaaaaatc atgtgttcat
      721 tttttatatt agttcgaatt tgcaaaaaat tgtatttgat aaagaggtga gtaaaaaaat
      781 aaaatagctg agcattggag gatagattga gtgatactaa gtctatcctc ttgtttttct
      841 aaagaactaa gctagaattt tttcttttgt acgttattat atgttatttg tcattaccaa
      901 aaagcacctt ttttcttcta gtatctatga cataagaaat accacccatt tcctttactt
      961 gaacaaatcc tactgattca taaaattttt ttgttttctc agtatcatct gtcaataatt
     1021 cgatttggac tggtccataa ctagtgatct tctccaaagc ttcattcaaa agcactttgc
     1081 ctattccttg gcgctgatac ctcggatgaa tgaggatatc ttgaataaat gcgatcgtcg
     1141 caaaatcagt gatccaacga acgattccga taatcttccc ttccccatta cgctttatca
     1201 gagattcaga ttgttcaaat gctctagcta accttgcagt gtcttttgtg taattcgtcc
     1261 aaccaacgct cttatataat tcagaaatct catcgatcat aagggaatca atattcattt
     1321 ttttcttcct ttcttataca attctccctc taataataca gttctttgac tatttaatct
     1381 atcacatata actctcctgt aaagattctc tcgattcctt aaaattcctc tttcctttta
     1441 gaatgcatac tttttgataa ataaaaaggt tgtgataaat tttcttcaca acctctgttt
     1501 ctctctatca agtgttatct tttaattttc tacccttacc ctagcatctc tattgcccgt
     1561 agtatgcata ctttccatgt ttgcgaaaat aatgtttatc tagtaaatat tgcgggattt
     1621 cacatatatt ggggttgatc agctcgtttt ctttggccat cagacaaatt tcatctaaga
     1681 tcaaactgtt ttctactgct ttcataggtg aatcccccca tgtgaacggt ccatgtccat
     1741 agacaagtac tccagggact tcgtttggat ctaattttct ttccttaaac gtctcgacaa
     1801 tcacattccc tgtatgaact tcgtaagctt ctcttacttc ttctttcgtt aattggcgtg
     1861 tacaaggaac cttaccataa aaagcatcag catgagtcgt gccataagct ggcaaatcgc
     1921 gtcctgcttg cgcccacatg acggagtgcg tggaatgagt atgagtaata gcagttatat
     1981 cttcaaaagt ttgatacaaa acaacatgtg tggggagatc tgaagatggt tttaagctat
     2041 cttcttctat tatatttccc gacaaatctg ttaccaccat ttgatctgct tgcatgcatt
     2101 catatctgac accgcttggc ttaatgacga tgactcctag ctcacggtcc acttcactca
     2161 cattgcccca agtcaatttg actaatccgt atcgcggcaa tgccagattt gcttcaagga
     2221 cacgttgctt cattgctgca attttttctt cacaagacat catagccagc ctccttcaat
     2281 ttagaactta agtagtcttt tgcctgctgg atttctcttc gaaagtcttg gcttttttca
     2341 ctccacattt caatcagaaa tgttccatca taatctagac gtttcaaatg tcttaataat
     2401 ccaagaaaat caacacaacc gctaccaaaa ggaacatcac gaaactggcc tgaatattct
     2461 tttgttacag ggtaagtatc ttttaagtgg atagcagtga tataatcgat ccctttctca
     2521 agttctcgag ccggattatt ttccggccat gcggacaaat tgcctaggtc tggatatact
     2581 tgtagaaaag gtgaatgaat ctgttctttg atttctaaaa attttgaaat ggagttcata
     2641 aatggatcat ccatgatttc cacggaaaga atgatgccat atttagaagc ttcctttacg
     2701 ctttctttta ttccttcgat aaagtcttct ctggaattca ttgatttctc ttcataataa
     2761 acatcgtatc cagctatttg gattactttg acgcttagat tatgcgccaa atggattgct
     2821 tttttcatta gttgtttcgc tgcttttttc ttttctggat cagcagaacc aaacggaaat
     2881 cgccgatgcg cacttaaaca aatcgaatgg atacgtacac cggagctgaa catctcttct
     2941 ctcagctcag ctatcttttc ttcagaccaa tctaatcgtg ctaaccgctc atccgtctca
     3001 tcaatcgaca tctcgataaa atcgaagtcc atttctttta caagggaaaa acgttctttc
     3061 catgaaatat ctttgggtaa cgctttttca taaatcccaa tttgagccat ttccttaccc
     3121 ccagatacga atgatctcat ctttgaagtt ttgtgcggcc ttcatcggat catttgctgc
     3181 tgtaatacca cgaccagtga taaaggtata cacatcgatc ccttcaaata agtgtaatgt
     3241 gtgtggattt aatccaccag ttacagaaac attaaagcct agttcaatca gttttttgat
     3301 cttcgataaa tctttttcac cccaagtttc accagataac agcgcatcac ggctttgatg
     3361 gtagattacc tgccggatac cgatgttatg ccaatccatg gcttgttcaa aagtccaatt
     3421 gccgtagagt tctacttgca attcacctat ttctttttgt gctgcctcca tagttggaat
     3481 cgttgcacaa caaatgactg tcatccagtc tgctcctgct tttgcaacat tactcgccac
     3541 tgttcctcca gcgtctgcac atttcgtatc agcgactagt tttttatctg ggaacatact
     3601 gcgaatacag cgaatcgctt tttgtccttc ctgtaagcaa agaattgtgc ccacttcaat
     3661 aatatctact acatcaccaa ctttcataac atctcctaat gcatgttcta agtcattgtg
     3721 atctaacgca atttgcaaat tcggcctgct catcttagtt ccttccttta cttgtttttt
     3781 tggtaccact ttgtttcagc aaatttttca ctgatttctt tgactgacat cacatttttg
     3841 ataccgataa ctgtaactcc ttttttctct gcctgagtaa acatattcaa aaaattgact
     3901 ggcgtaaaaa ctacatcgta ttggccagct gaactttttc cctctgaaat ggcacaatga
     3961 tggattttgg taattggtac gtttagttct ttcattgctt tttccacact tctcatcatc
     4021 atcaagcttg ttcctgatcc atttgcacac gatactaaaa ttctcatctt aatccctcca
     4081 ttatgagtgt tgtctaatca gattttcttt tatactctct gcttttctgc atatgtgtca
     4141 tagtcttcta caatcaaaaa ataaccatca ggatttttcc gatattgtaa ctgcggaata
     4201 gccaataaac caatgacaac tacggcaatt ccagcataac ctaggacttt catgattact
     4261 gtaaacaatg gccaagctgt tgcccagtcg aacatcccga tataaccacc gtatgttgac
     4321 atcccaatcc atgtagcgaa taatgctgaa ccgcctactt gtatgattcc tgaaagaaac
     4381 gggaacaggc aagcggcttt gaatccacct cggttattgg cataaactgc tataacggca
     4441 ttatcaaaga acaacggaat gaaaccggct atgacgatcg ttggtgattt caataaaatc
     4501 agtaaaccaa tcatcaaaaa ctgtcccaaa gcaccaaaca agaacccaat cgttaccgca
     4561 tttggcgaac caaagccaaa agtagcagct acgtcaattc ctggaacagc tcctggtaaa
     4621 atcgtgtttg agattccttg gaaagattga gttagttcat cgacgaatgt ccgaacacct
     4681 aattgcaaga tcgccagata taccgcaaaa tttaaagaag tcgtcataat gtagaataag
     4741 aaactctgcc cttcttgcat gaattgcgct tgaatcaaat aatctttgcc tagtacaatc
     4801 agaatgatcc caaagaaaaa tagcatgagt aacgaagtag caaccatatt ctcattgaaa
     4861 atcgacaaga atcctggtaa tttgatatct tcaatttttt tgtcttgctt gattgatcta
     4921 tttttctttt catcccgctt tttcatccac tcagctaatt ttgcaaatat aaagattccg
     4981 aacatttgct gatgtgccac tgcaaatccg gcaccttctg tcaattcctg cgtgattcct
     5041 actgttaagt tagaagctac tgtccaataa caacctaaaa tcagtcccat aaaaatcaat
     5101 gcttgtatcc tacctagatc aggaaagcaa aacagtaaaa tccaaaaagc ggtagctgct
     5161 tgttgaacct gaacgtttcc ggtagtgaaa acggaacgca gtttcgtata tttctgaaaa
     5221 cggactaata agatatttaa aacaaatgca atcagtaata aaatcatcac atctccaaag
     5281 gtacggccaa atgtctccat taatccagca tccacagcat tttgtccaaa ataaggatct
     5341 gtaaccatgg catccaaatt gaaccgttcc tttagtccaa ccaagattgg ccgaaaatta
     5401 ttgaccaaac caccagagcc aacagtcaaa ataaagtaac cgaccgttgc ctttaagaat
     5461 ccagcgatgc attcatgaag cgggcgtttc agcaaaatat agcctaataa cacgataaaa
     5521 ccaatcaggt atgctggctg cgtcaagata tttgttgcaa aatacgtcca aatgcttaac
     5581 aaaaattccc ccatcttttt tctcctctat caattaatta tattctgcca tcacaacttg
     5641 gtaatcatcg atggagttcg tagagatgag acgctcaacc aatccatctg tcattaataa
     5701 ttcggataat ttttggatat tttctagatg ttcttctgga tcttttgctg cgagtgtaaa
     5761 aaacaaacta gccgttttct cctcattctc cccttcactt tcaaaacgga ttggctgttt
     5821 caacttggta aatgaaatag ctgttccaaa aacaccatca cttttttctg aagaatgcgg
     5881 cattgctact tgaggtgcga tcacaatata aggaccgtat ttttctacac aatggatgat
     5941 ctcctccaca tacgtttcat caataatttc cttatcgatc aatacaagac agctttcggc
     6001 gatagcttca cgccaatccg tgatttcttt atctgggtaa tgaatcaatt gatggtcaaa
     6061 aaagtatttc agcaagaaac tccccttctt tcttataaaa acgacggaaa cggtgtatca
     6121 ttttcaatcg agaagacatc ttcaaatcct cgataaaaat taaactcaag atcatctttg
     6181 tttgtcggat agatgaattt cccgcctact tgccagatat aaggtttaaa tccatacttc
     6241 aggcgatcct ttttgaattt ccaaatttca gtgatctctt ttggatcagc cataaaatta
     6301 gaccaaatat cgtagtggac aggaatcact acttttgcat tcaatgactc cgccatgcgc
     6361 agcatatcaa cagaagtaac tttgtctgtt atccctcgtg ggttttcacc ataggcccct
     6421 aaacagacat cgattttgtg ttcattacca tgtttggcaa ataaattgga gtaatgtgaa
     6481 tctcctgcat gataaagatt ccctcctgat gtttcgaata aatagtttac tgcaatttga
     6541 tccatctcct gaggcatttt tccttttaat acctcttctg gatcttcaca agtgaccaac
     6601 gctgtccgat caaacgcttc taaagctact acattgatgt ctttgatttt gactgcatca
     6661 cctggcttta ctgtaatcgt tttttcttca ggaattcccc atttttgcca cgtgcgaacc
     6721 acttcttttg gcccaatgaa tgttgcttca ggacaatttt gatgaactgc tgcggctgta
     6781 tggatatcta gatgatcgga atggatatgt gtaacgatta aagcgtctac attttttatc
     6841 gcaaaaggat cgattacgaa aggctgagta cgtaaattag gctgcatatt ttgaacgcca
     6901 ctcatacgca tcatctgatg gccttttttc atcatgccat tcccatgact acgttttccc
     6961 gtcccacacc atagatcaca taaaatattc gttccttcat gacttttcag ccaaattccc
     7021 gtacagccca gccaccacat agcaaaagtt ccaggatcca ctacttctgt ttcgatttct
     7081 tcattcaagt atgtgcccca ctcaggaaat gtttctacta accacttttc ttttgttact
     7141 tcattgacag ttttactcat ttgcttgctc cccccatatc gttgttccaa cttacaagta
     7201 aatattaatg tgtatcagca tccatgaaaa cccttttata tgaccgtttg ttttttatat
     7261 gattttcttt cagtaatcaa tgattgtttc agtataataa agacagatat gaatgtttaa
     7321 atgaacaact agtcaaataa atccatggat caagatgtcc ctcaggaggc aaaagtcatg
     7381 aaaaactcac ttgtcaatat tgaaaaacgt caaaaagaaa ttttggaact gcttcagcag
     7441 actgaacaac tatcaacttt cgaattagct gaatcccttg atgtatctat tagtacgata
     7501 cgaagagatt taaatttatt agaagaaaaa aacgagatca tacgcaatta cggttattgt
     7561 tcttataatt acaaaaacca gactgatttt gatcaatctg gtcctgaaag gattaaacaa
     7621 gcgattgcca gagaagctag taaatacatt aacgattatg atacgctgtt tatcaattct
     7681 agctcgactg cgttgaaaac actggatttt cttcaatcta accatttgac agtcattaca
     7741 aacaacgtga aaatcaatta ctctccgcac aaagataatt gtagttatat tttaactggt
     7801 ggagagatgc gctttccaaa agaagcacta gtgggcgaca tcgcattaaa tacgatttct
     7861 tcagtcaatg cagacatttg tatcattggc tgtagcggag tcgatgtagt acatggggtc
     7921 acaacaaaga tattaaatga atcaacgatc aacgaaatga tggtcaacaa gacaatcaaa
     7981 tgtaagattt tagtcgctga tcatcgaaaa atcggactga catcaaagtt caagattgct
     8041 gatatttcag tctttgatta tttgatcact gataaatttt gttctgaaaa agtattagag
     8101 gagatcagaa atacggggat aaaaaccatt caagtagatt cattttaaga accaggacgt
     8161 aaatatgtaa ataggattat caaaaaaatg aataacaaat ttatcgataa atgaaaaaca
     8221 cgctacgctc aaattgataa aggaatccca agatgtatcc atccaatcga taccatcata
     8281 atctattgct tcttttataa ccgttatatg ttgcctaaat aggaatcaaa caatatcgaa
     8341 aaaaggaggt atgacaaaag tcacacctcc ttttttcgaa taaacggtat tcaaatcaga
     8401 acactgattt cacaaccact ttatttcagg tcgttttcaa tcacctttac cgcgtaatca
     8461 tttccgttga aacgcgctat tttttttgcg tccatatatc tttctaaata atcatcttct
     8521 cctaattgct tatggcaaga agcaatttca taaagcaagt attccaatgc ataagtcgat
     8581 ttatgtttct tattatactc aatacccttc tcacataatt ggattgcttt atcataatcc
     8641 tcgattttac ggtaataggt ggcgatatca taatatacaa tgcagaccat cagattatca
     8701 aaatcagtct cactcaatat tttaagtgta cgctccacat aaactttcgc aaaatctaat
     8761 tctccttttg acaagtagat cattgccacg tttgcagctg cttgtaagtg atggatatct
     8821 gtttcggtcg tttgttccaa cacttgatta aaataaaata atgctgcatc tggttttttc
     8881 gcataaagat gatcaagcag tccttgataa aataaaattt gatttaaaag atcagaatta
     8941 cttacttttt cctgatcgat cgtttgtaaa attttataag attcttggta ttttgataca
     9001 gaattcaatg attgaatctc atttattttt ttcctgattt tgtcttcttc tgtttctact
     9061 aatatatcgg agatctcgac attcagcttt ttgcaaagcg cacttaagat attgatgcct
     9121 ggcgacatat tcttgttttc gatattgctg acagttgctt gttcacaaat gccttctgct
     9181 aattgtactt gtgttaaccc ctttttcttt cggtattctt ttagtaatgc tccatctact
     9241 ttaatcaaaa caatttcctt ccctttaaaa aattatttct cctttatatc atacacaaaa
     9301 aaaagttcta aacaaccaaa aatgtttaga acagaataaa aattttaatt ttaaaataat
     9361 attcaaatta aacaactggc gtatctgctc cttgatttct ttttgtgtta tctgaataat
     9421 tatcgtttac agttataaca catagtgaaa attgtcatgg cgagaaagtc taagaatatt
     9481 tttaaacata actatttctt tcttac
//
LOCUS       DAYYKN010000103         9453 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000103 DAYYKN010000000
VERSION     DAYYKN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.104"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(184..852)
                     /locus_tag="ACHBB7_002166"
     CDS             complement(184..852)
                     /locus_tag="ACHBB7_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9210087.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            986..1273
                     /locus_tag="ACHBB7_002167"
     CDS             986..1273
                     /locus_tag="ACHBB7_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210088.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1689..3227
                     /locus_tag="ACHBB7_002168"
     CDS             1689..3227
                     /locus_tag="ACHBB7_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM9210089.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3224..4189
                     /locus_tag="ACHBB7_002169"
     CDS             3224..4189
                     /locus_tag="ACHBB7_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9210090.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4176..5498
                     /locus_tag="ACHBB7_002170"
     CDS             4176..5498
                     /locus_tag="ACHBB7_002170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9210091.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5561..5665
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5739..6482
                     /locus_tag="ACHBB7_002171"
     CDS             5739..6482
                     /locus_tag="ACHBB7_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9210092.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6497..7489
                     /locus_tag="ACHBB7_002172"
     CDS             6497..7489
                     /locus_tag="ACHBB7_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9210093.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7501..8238
                     /locus_tag="ACHBB7_002173"
     CDS             7501..8238
                     /locus_tag="ACHBB7_002173"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9210094.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8228..8950
                     /locus_tag="ACHBB7_002174"
     CDS             8228..8950
                     /locus_tag="ACHBB7_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM9210095.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(9034..>9453)
                     /locus_tag="ACHBB7_002175"
     CDS             complement(9034..>9453)
                     /locus_tag="ACHBB7_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM9210096.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3109 a         1619 c         1983 g         2742 t
ORIGIN      
        1 gtttaagcga attttttctc ccaataagta agaaaataat ggcaaaataa aaaaacaaac
       61 tattttcgca gatcgtttgc aaaaatagtt tattttttta taaagaagat tttactgaat
      121 aatatcattt tagtttacat aatgatattt ctgtaaatta aataaagtag tgaaaaataa
      181 gaatcagtgc tctaactgtt caagaggatc aagtggttca aatgtttctg tagtctgttt
      241 tgcttccaat tggggcaaaa cagatccaat ttccagatac ttcactttat tcaatgtata
      301 agatagaata gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa
      361 ggagacaact aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa
      421 atatttttca atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa
      481 gacgagaccg caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact
      541 atctacagca aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa
      601 tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa
      661 taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa
      721 aagcaaagca ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt
      781 cacagctaat ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt
      841 taattttttc atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac
      901 tgacttatac tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt
      961 tatttatcaa ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa
     1021 atcacccaaa aaaacagagt atcaaggagt ttttttggct caagaacaaa aactggtgcc
     1081 tgtgacaatg gtccagataa ataaagcaaa tcaatttatc tttttatttg aacacggcaa
     1141 agcaccactt gctatgaaag acatctttct ttttttgatg aagaaccgtg agaaagctgt
     1201 ttattatcag tcaaaagaag aactcattcc tctttatgga gtgaaagaga tagaaaataa
     1261 agtggtgata taagtaaaaa taagaaaaca agacgtttat gatgtcaaaa caaccatttt
     1321 ttacattttt ggcaaaaaag tgtcatgata cagtataata ttcgttgaac aggccaataa
     1381 aggtgttctc ccccacgaga tttctacttt attggctgtt tctttttatt aaaataaaag
     1441 ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag atttttatgg
     1501 tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct tggattcttt
     1561 ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt aaggttaagg
     1621 caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat aacagaaagg
     1681 aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag ttgctggaag
     1741 ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca ctgattgcta
     1801 ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta ttgcagatca
     1861 cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc ccatttgtag
     1921 ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt ccgattttcc
     1981 aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca gcggtagcat
     2041 ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca ttacctgtca
     2101 ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact ttggcagcat
     2161 tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga aatacacctg
     2221 ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc agcggactga
     2281 ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca gcagtggtgt
     2341 gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa accatcacga
     2401 ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa gaattgattg
     2461 aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg agttttttgt
     2521 tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt actgtcctag
     2581 aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat
     2641 atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta aaaccaatgg
     2701 cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa catcaactta
     2761 aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc acactggaat
     2821 ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg aatttttcaa
     2881 gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga gatgtcaata
     2941 ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg actctagaag
     3001 atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta
     3061 aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata acagaggatc
     3121 aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac
     3181 gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta aattaatcga
     3241 gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc taaatctggc
     3301 aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa aaacaactac
     3361 tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact tcaaaggaaa
     3421 atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg tgctgcagca
     3481 aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt
     3541 aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa aagttggctt
     3601 atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag aacagcaacg
     3661 gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg acgagccatt
     3721 tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt ccttacacga
     3781 agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa tcaagctagg
     3841 tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac cagagcagtt
     3901 attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga aacagacgaa
     3961 agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa
     4021 tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt
     4081 gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga caagtaaaga
     4141 aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat gcaatcatca
     4201 tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca ggtaaaacag
     4261 tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga tgtgatccta
     4321 aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg agtggaaaag
     4381 gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa cgaacattta
     4441 cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc gatcatcttt
     4501 caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa gtcttccatg
     4561 cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg gagggaaagg
     4621 aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa gaaatcattt
     4681 ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct gcagggagca
     4741 aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg tctttggtag
     4801 aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt gttgaaaccc
     4861 aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg gaattagtag
     4921 cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta gcacttgagc
     4981 aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg caaacgagca
     5041 atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa ttgacaccag
     5101 tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca agcgaaccta
     5161 tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct gaagtaggag
     5221 ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt accagttggt
     5281 tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt gaccagcaaa
     5341 attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc gatgatttga
     5401 cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc atggggtatc
     5461 cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa ctaaagcgct
     5521 tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc tggggatgtc
     5581 gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag ttaatactgg
     5641 tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc atttcaagcg
     5701 ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat ctttatccag
     5761 cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt tggaaaatac
     5821 ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggca gatgcacaag
     5881 tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg gtaatagcta
     5941 gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta gaaatgtctt
     6001 tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg ccattactta
     6061 ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg ataagctatt
     6121 ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag caaatcatct
     6181 ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa ataccagcgg
     6241 gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc agcggagcag
     6301 tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata cgcgtaaaaa
     6361 acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta ttgagtttat
     6421 tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt tatatttttt
     6481 aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt tgcttttatc
     6541 cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt
     6601 gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc tagcaaaaga
     6661 aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag gagataattc
     6721 gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga
     6781 tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg ctacagtgat
     6841 caaacataat acttctggat tcgccaccct atcagacagt aacatccatt caccaaaaga
     6901 ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg tcttaaaagc
     6961 tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa gcaatggaga
     7021 agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag gttgggatat
     7081 gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag aattagatga
     7141 acggttagat tactatactc cggtaattat tacgaatgat caactgatca aaagtgatcc
     7201 agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag ctattaagga
     7261 tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt
     7321 agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga attggggatt
     7381 gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg gattgatcaa
     7441 gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg agtgatagat
     7501 atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat attagaagac
     7561 atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag cggagccggt
     7621 aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg aacgatcaaa
     7681 gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc gcaagaagat
     7741 atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat
     7801 ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt aaacggctat
     7861 gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc cttgttacga
     7921 acaatcatga atccttcttc ttacttgctt cttgatgaac catttggagc gttagatgct
     7981 atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc aaagcggact
     8041 actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag gattcttgtg
     8101 ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa acgacggtca
     8161 cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt attggcaggt
     8221 gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt tagcactcct
     8281 tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag aatggaagga
     8341 aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc atccttggaa
     8401 agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa ggatcatcgg
     8461 tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa aaaaagtatt
     8521 tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc acacaaaagg
     8581 atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt tgatccattg
     8641 gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt tcagcattgg
     8701 tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc caatcgacca
     8761 cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg ggattttttc
     8821 agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg agatcaaaga
     8881 gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt
     8941 aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg ctttctttgt
     9001 ataaaagtaa actgtttgat taataaatga atcttagtac caaccgtttg cttcccagaa
     9061 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
     9121 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacgtccgat
     9181 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
     9241 tgcagagctt gtgcttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
     9301 ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa caggttgttc
     9361 agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc
     9421 ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAYYKN010000104         9432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000104 DAYYKN010000000
VERSION     DAYYKN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.105"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..680
                     /locus_tag="ACHBB7_002176"
     CDS             <1..680
                     /locus_tag="ACHBB7_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9210097.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="ACHBB7_002177"
     CDS             800..1000
                     /locus_tag="ACHBB7_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9210098.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="ACHBB7_002178"
     CDS             1076..1273
                     /locus_tag="ACHBB7_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210099.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="ACHBB7_002179"
     CDS             complement(1570..3117)
                     /locus_tag="ACHBB7_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFM9210100.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="ACHBB7_002180"
     CDS             complement(3201..4004)
                     /locus_tag="ACHBB7_002180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9210101.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACHBB7_002181"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACHBB7_002181"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFM9210102.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACHBB7_002182"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACHBB7_002182"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFM9210103.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACHBB7_002183"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACHBB7_002183"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFM9210104.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="ACHBB7_002184"
     CDS             complement(6389..7318)
                     /locus_tag="ACHBB7_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9210105.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="ACHBB7_002185"
     CDS             7513..8196
                     /locus_tag="ACHBB7_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFM9210106.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9400
                     /locus_tag="ACHBB7_002186"
     CDS             8357..9400
                     /locus_tag="ACHBB7_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9210107.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaag attttacgta
     8581 tatagaaacg aacaatccag aggataacag taccaatatc gatcaggcaa tcagaaacgg
     8641 ctttaccacc attattagct tgagctactt gcaagcagag gaactggaaa atgctgctaa
     8701 agataatcca aagttgaaat ttgccttggt agatggtgag attactggga aaaagaatat
     8761 ctcatcagca acctttaaag atcaagaaag tgcgtattta gcaggactag ctgccgcata
     8821 tacgacaaaa agtgatcaag taggctttat tggtggaatg gaagggcccg tgatccaacg
     8881 tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc acagcactga acaaggaaat
     8941 tactgttaac gtacaatatg ctgcatccta tgattcgcta gataaaggaa aagctattgc
     9001 tgcttctatg tacaaagagg gagtcgatat tctttatcag gcggcaggaa gtactggagc
     9061 tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac tatagcagaa aagtatgggt
     9121 gattggtgta gatcgcgatc aagcggatga aggggattat aaaatggaga acggcgagac
     9181 agggaatttc acgttgactt ctacaaagaa aaatgtcggt gcagaagtca aagaatttgc
     9241 agaaacttct cataacgata aatttcctgg cggcaagcat ttgacctttg gtttgaaaga
     9301 agaaggggta gatctgacaa aaggacaatt atcagaggaa gcctatcagg cagtttcaga
     9361 cgcaaaagag aaaatcatca gtggtgattt cagactgtag acaaagtcca ttttatggat
     9421 gatgtctaca gt
//
LOCUS       DAYYKN010000105         9376 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000105 DAYYKN010000000
VERSION     DAYYKN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.106"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            277..1887
                     /locus_tag="ACHBB7_002187"
     CDS             277..1887
                     /locus_tag="ACHBB7_002187"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM9210108.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1864..2076)
                     /locus_tag="ACHBB7_002188"
     CDS             complement(1864..2076)
                     /locus_tag="ACHBB7_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210109.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            2179..3048
                     /locus_tag="ACHBB7_002189"
     CDS             2179..3048
                     /locus_tag="ACHBB7_002189"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM9210110.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3213..4484
                     /locus_tag="ACHBB7_002190"
     CDS             3213..4484
                     /locus_tag="ACHBB7_002190"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9210111.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4481..5776
                     /gene="rho"
                     /locus_tag="ACHBB7_002191"
     CDS             4481..5776
                     /gene="rho"
                     /locus_tag="ACHBB7_002191"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM9210112.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            5875..6138
                     /locus_tag="ACHBB7_002192"
     CDS             5875..6138
                     /locus_tag="ACHBB7_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM9210113.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6583..7032
                     /locus_tag="ACHBB7_002193"
     CDS             6583..7032
                     /locus_tag="ACHBB7_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9210114.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7089..8507
                     /locus_tag="ACHBB7_002194"
     CDS             7089..8507
                     /locus_tag="ACHBB7_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9210115.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8507..9367
                     /locus_tag="ACHBB7_002195"
     CDS             8507..9367
                     /locus_tag="ACHBB7_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM9210116.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
BASE COUNT         3126 a         1628 c         2063 g         2559 t
ORIGIN      
        1 aagcttatta tgtgggttaa attttttttg cactcataaa ggacttaaga aataatttct
       61 caaaggatat cgaaaaagaa aaaattgtaa gcggatagta ttcatttgag tacttgactt
      121 tcccttagtc agcttgtatt atacaccttg ggcacccttt tagagggtca gaagtttaca
      181 gaaatttcgg ctccctattc ttattgaaga atagggagct ttgtttattg tctgagactt
      241 ttcctgcaca taaattaaaa aggagttttt gaacttatga caaaatatat ttttgtgacc
      301 ggcggtgtcg tttcatcaat cggaaaaggg attgttgcag cttctttagg acgcttattg
      361 aaaaaccgcg gcttaaaagt aactatccaa aaatttgatc catacatcaa cgtggatccg
      421 gggacgatga gcccatatca gcacggagaa gtattcgtaa cagatgatgg tgctgaaaca
      481 gatttggatc tgggccacta cgaacgtttt attgacatca atttgaacaa atactccaac
      541 gtgacgacag gaaaaatcta ttctgaagtt ttgcgtaaag aacgtaaggg cgaatacttg
      601 ggagcaactg ttcaagttat tcctcatatt acaaatgaga tcaaagaaaa aatcatgcga
      661 gcagctaaaa tgactgattc agatgtgatc attacagaag tagggggaac agtaggggat
      721 atagaatctt tgccattcct tgaagcactt cgtcaaatga aagcagatgt cggtacagat
      781 aatgttatgt atatccatac aacattgatt ccttacttga aagctgctgg agaaatgaaa
      841 actaaaccaa cgcagcacag tgtaaaagag ctacgtggtt taggtatcca accgaatatt
      901 ttggtggtac gtacagagca acctgtttct caaagcgtga aaaacaaatt ggcacaattt
      961 tgtgatgtcg aaccagaagc agttatcgag tcgccagatg tagatacact ttactccatc
     1021 ccattagttt tacaagcaca aggaatggat caaattgttt gcgaccattt aaaactagat
     1081 acaccagaag cagacatgac agaatggaaa gaattagaag aacgtgtctt gaacttgaag
     1141 aaaaaagtac ggattgcgct tgtcggtaaa tatgtcgagt taccagatgc atatatctca
     1201 gtagttgaag cattgaaaca ttctggtttc gcctataatt cagacatcga gatcgattgg
     1261 atcaaagcac aagaattaac gagagaaaat gtagaagaac gtttgaaaga cgcagatgga
     1321 atcttggttc ctggtggatt tggtgatcgc ggagtagaag ggaaaatcga agcgattcgt
     1381 tatgctcgcg aaaatgacgt tccgttttta ggtatttgtc ttggtatgca gatggcttgt
     1441 gtcgaatttg cacgtaacgt cgttggatta gaagatgcgt catcagctga aacgaatcca
     1501 gatacagcaa acaatattat tgatttaatg gctgatcagg aaaacatcga gaatttgggt
     1561 ggaactttac gccttggatt atacccatgt aaaatcaaaa aaggaacgaa gacggctgaa
     1621 gcatatgatg gagccgatgt tgtgcaagaa cgtcaccgtc accgctatga gttcaataac
     1681 aaatatcgtc aattgtttga agaacaaggg ctagtctttt caggtgtctc accagataac
     1741 cgtttagtag aaatcgtaga gctgccagag aaaaaattct ttgtgggatg ccaattccac
     1801 ccagaattga tttctcgtcc aaatcgcccg caaaaattaa tcaaagcatt tgtcggagca
     1861 tcattagcag atcacgaaac aaaataattt ttgaacttga tagaaagaga aaagtagcaa
     1921 gagaagggga taagcaattt tcttgtcact tttctctttt tctgatttgt tattatttgt
     1981 aggaaaaaag atagaaagca aaagactttt ttcgattaaa gcgctattgg atattttatt
     2041 ttttgcaaaa aacttcttga aaattttcat tttcattgat tttagtagaa aactaccgaa
     2101 atgtttggta aaatagggtc gttggttaag aattgtctta acaaatttag ttttacaaaa
     2161 cttaggagga attttattat gccagtagta tcaggagctg aatttttaaa agctgctcgt
     2221 aaaggcggtt acgctgtcgg cggattcaac acaaacaact tggaatggac tcaagcaatc
     2281 cttgaagcgg ctgaagcaaa acaagcacca gtacttatcc aaacatcaat gggtgcagct
     2341 aaatacatgg gtggttacaa aatctgtaaa gatttaatca ctgatttagt tgaagcaatg
     2401 aacatcactg ttccagttgc tatccactta gaccatggtg attacgaagc tgctttggaa
     2461 tgtatcgaat taggctatac ttcagtaatg ttcgatggtt cacatctacc atttgaagaa
     2521 aacttgaaat tagctaaaga cgttgttgaa aaagcacatg caaaaggtat ctcagttgaa
     2581 tgtgaagttg gttcaatcgg cggagaagaa gacggaatca tcggtaacgg cgaattagct
     2641 gatattgaag aatgcaaaca aatggttgct acaggtatcg actacttagc ttgcggtatc
     2701 ggtaacatcc atggtcaata tccagaaaac tggaaaggat tagcttttga ccacttacaa
     2761 gcaattgctg atgctgtagg tccagacatg cctttagtat tacatggcgg atcaggtatt
     2821 cctcaagatc aaatcgaaaa agctatctca atgggtatct caaaagtaaa tgttaacact
     2881 gaattccaat tatcatttgc tgcagctact cgtaaatata tcgaagctgg taaagataaa
     2941 gaaggtaaag gattcgaccc tcgtaaatta ttagctccag gtaaagcagc aatcgttgaa
     3001 gatgctaaat ctcatatcga ctggttcggt tcagctaaca aagcgtaagc atccacataa
     3061 cagatacgat atataggaat ggagaacgac ttttgaagtc gctctccatt cttttttact
     3121 taaaacggca gaccatgctc gtttcttttt tttatctatg ataaaataca agcatatcca
     3181 tgaaaaaaga ttaataaaca aggtggaaac agatgaaaaa aatcgtaatc aaaggaaacc
     3241 gacctttagc tggtgaagta acgatcagcg gtgcaaaaaa tagtgcagtc gcattgattc
     3301 cagctgcaat cttagctgat tccccagtga tcttggaagg tgtgccggac atacaagatg
     3361 ttcattcatt aattgaaatt ttagagatca tgggcgcaac tgtccatttt tccaacaaca
     3421 tactagaaat cgatcctcga gaagtcgtat cgattccgat gccgcacgga aaaatcaata
     3481 gtttgcgtgc ttcttattat tttatgggaa cactgcttgg taaatttggc gaagcggtag
     3541 taggcctgcc aggtggctgt tatttaggcc ctagaccgat tgacctacac gtcaaaggat
     3601 ttgaagctct aggtgctgaa gtgacaaatg aacatggtgc catgtattta cggacagaaa
     3661 ataaaggatt gcgtggcaat cggattttca tggacgtggt ttcagtcgga gcaacgatca
     3721 atgtgatgtt agctgctgta aaagcaaaag gacagacagt aattgaaaac gctgcacgag
     3781 aaccggaaat catagatgtg gcaactttac tgaataatat gggggcaaaa gttcgaggag
     3841 cgggtacaga tgtgatccgt atcgaaggag tcgaaacatt acatggctgt cggcatttca
     3901 tgattcctga ccgcatcgaa gcaggaacct atctagcatt agcagctgca gtaggaaatg
     3961 gtatcaaagt gaaaaatgtg atctttgaac atttggaaag ctttatagca aaattgcagg
     4021 aaattggtgt ccatatgaaa atcagcgaag atgaaatcga agtttacccc tcgaaagaat
     4081 taaaacccgc aaacatcatg acctatcctt atccaggatt tgccacagat ctgcagcagc
     4141 cacttactgc gttactattg atgactacag gcacttcaga aatcatcgat acgatctatg
     4201 cgaaacgtgt gaaccatgtg ccggaactag cacgaatggg cgctgatatt accgtggaag
     4261 gaaatatgat catcgtgaat ggtccaaata aacttcacgg cacagaagtc gtggcttctg
     4321 atcttcgggc aggtgcttgt ttagtgattg ctggactatt ggcagaaggg acaacgacga
     4381 tttacaatgt agattatatc ttacgcggtt atgatcatat tatcgagaaa ctaacagcct
     4441 taggggcatc aattgaaatg atagaggaag tgggagctga atgagcgatt atttgacaat
     4501 ggcagaacta gagaacaaaa cgttaaaaga agtttatagt tatgccaaag aatttaaaat
     4561 tccttattac agtaagatga ataaaaagga attatctcta gctgtgattc gtgcacaagc
     4621 agagaaacaa ggattttatt atatggaagg aatattggat atcgttggtc aagatggtta
     4681 cggtttttta cggccaatca attatggacc aagtgcagaa gatatctata tttctgcctc
     4741 acaaatcaga cgattcagtt tgagaaatgg ggacaaagtg gcaggaaaag cccgtccacc
     4801 taaagaatcg gaacgttatt atggtttgat gcacgtggaa agcgttaatg gaaaagatcc
     4861 ggaagaagca aaagaacgtc cgcattttcc agccttaaca cctctttatc cgcaaagaca
     4921 gcttcaactg gaaacaacac cgcaaagact atctacccga atgatcgata tcttttctcc
     4981 aatcggtttt ggtcagcgtg gattgatcgt cgcaccgcca aaagcaggga aaacttctgt
     5041 attaaaagaa atcgcaaatg gtatcaccga aaactatccg gaagtagaat taatcgttct
     5101 actgattgat gaacgaccgg aagaagtaac ggatctagaa cgaagcgtga aaggcgacgt
     5161 ggtatcttct acatttgatc tgcagccgca aaaccatact cgagtttctg aattagtatt
     5221 ggaacgcgca atgcgtttag tcgaagataa acgagatgtc gttatcttga tggatagtat
     5281 tactagactt gctcgggcct ataacttagt cgttccacca agcggacgta cattgagtgg
     5341 cgggattgac ccagccgcat tgtataaacc taagaaattt ttcggggcag caagaaacat
     5401 cgaagaaggt ggcagtttga cgattttagc gaccgcttta gtcgatacag gaagccgtat
     5461 ggatgacgtg atctatgaag aattcaaagg aactggaaat ctagaattac aactttcacg
     5521 tgaattatct gaacgtcgga tcttcccagc catcgatatc aaaaaatcag gtacccgaaa
     5581 agaagaatta ttgatgccaa gtgaagaatt agaagaaatt tggaaacttc gaaaaaatat
     5641 gactggagat tcattagaat atacggaaca actgatccgc tttttgcgta aaacaaaaaa
     5701 caatcagcaa tttttcaaag aatttcaaga tgtctccttt ggtaaaaaaa atccgaccag
     5761 aaatctgaaa agataattgc aaccttgact gaaacatgtt aagatgttaa tgttgtaaat
     5821 cgacaaatca actctggttc agcaaatgga gcagggcaaa ggagcgaaaa aatcatgaaa
     5881 gaaaacatcc atccagatta ccacccagta gtattcatgg attcaacaac tggtttcaaa
     5941 ttcttgtcag gttcaacaaa aacttcacaa gaaacagttg aatgggaaga cggtaataca
     6001 tacccagtca tccgtgtcga agtaacttct gactcacatc cattctacac tggacgtcaa
     6061 aaattcacac aagcagacgg acgtgtggac cgtttcaaca aaaaatacgg tctcaaagac
     6121 gcaaacgctg cggaataata aaaacgctga taactcaacg tttagcagac tatctgatag
     6181 ggatagtctg tttttttatg ttttttgcca atttaaaact tcgtttgatg ctgaaaaagt
     6241 cgtaggatca taatgcacaa aacaatgaaa aactagtata tagttttaat tatccacttg
     6301 ataaaaacac cttatattaa cgaaatgaag aggggttatt tcgaagtaaa aaaccattta
     6361 cctaaaatta gaagctaagc tttcctttca aggaaggctt tttgttttgt tatagacttg
     6421 cgattgtttg tgagctagat cgttcatttt aatgacttat acacgcggat gatgggtctt
     6481 tttcctctgt tcattcataa tattgaacgg tacagctagc gatgctatac tgacctcagt
     6541 tccaagaaaa cgttaacatt ttttgtgggg gagaggtttt ttatgaggtt tgatcaattg
     6601 atcgcagaag aactgattgt tttcgatcca tcgatagatt caaaaggatc attatttgag
     6661 cgaatagggg atgtgttgaa aaaaacaaat aaggtaagaa atgttcaaaa agtcgttaag
     6721 gcactattca aacgagagga ggagatctcc acaggaatag aagatggatt tgggattccc
     6781 catgcaaaaa gcaaatatgt gacaaaacca gcactgtgtt tttttcatac agccaaatta
     6841 acggactata gcgggataga tgaaatgcca attgaatata tttttgccat cgtagttcct
     6901 gaaaaatcag cagatatgca tctagagatt ctcagcacac tctccagacg attgatgaat
     6961 gcagatttta gaaagggaat aaaagaagcc actacgccag cagatatcat cagacagatt
     7021 agtcaagaat gaaaatagta gagtctttgt ccgagtatcg atagtgaaga aaaaggagga
     7081 aaataatcat gaaaatcgtt ggtgtaactg cttgcccgac tggaattgct catacttata
     7141 tgtcggcaga aaaattagaa gtaacagcaa gaaatctaaa ccatcaagca aaattcgaaa
     7201 cacaaggtgt aaaaacagaa aatcagttat ctgaacaaga aatcaaagag gcagatgcaa
     7261 tcattcttgc agtagataaa gaaatcgaat tggaccgatt tgccgggaaa aaagtaaagc
     7321 gtgtctctac aagccgagca atcaaagaac cgcaagtagt aatagacgaa gcattgagag
     7381 atattggcgt attcgttgtg tctaatgaaa aagaaccagc tgcaaatgag aaaaagccga
     7441 caatctataa tcatttcatg aatggtgtga attacatgct tccatttgtg atcgcaggcg
     7501 gaatcatcat cgcactgagt tttgcctttg ggattacagc ggctgatccc aactcagcag
     7561 actataatgt gttagcagct gcattttcaa ggatcggagg agatacagcg tttgccatga
     7621 tggttcctgc gttaggcgcg gggattgcta catcgattgc tggaaaagca ggttttgccc
     7681 caggaattgt tgccggatta ctggcctcta caggtggttc aggttttctt ggcggaatga
     7741 ttggtggcgt gctagcaggg tatatctgtg attttctggc aaataaaatt agcgtaaaaa
     7801 aagaattttc tgctatttat cagctgattg tcgtcccctt tttatcgatc ttaattattg
     7861 ggcttttgat ggtatttgtg attgaacaac cgatcacatg ggtgttggat gctttgacaa
     7921 attggctgaa tagcttggga aatacatccg ggctattgtt cggattactt atcgggatca
     7981 tgatggcagc tgatatgggt gggccgatca ataaatcgac ttcgactttc tcgatcggtt
     8041 tgatgtcagc tggagtaaat gcaccgattg ctgcttgtat ggctgcagga atgacacctc
     8101 ccctaggtat tgctttagcc actttattat tcaaacataa atttaccaaa gaagaacgga
     8161 cttcaggtca atcatgctgg attttaggcg cttcgtatat tacagaaggt gctataccgt
     8221 ttgcggtatc tgacccgatc cgcgtgatac ctagtttgat gacaggttca gcagtcgctg
     8281 cagggatttc catggcagta ggagttactt caatggcacc tcatggaggg atttgggtga
     8341 tgctaattcc aaatgttatg aacaatttac ctatgtatgt cttggcaatt gcatcaggta
     8401 cagtcactac agcctttatg cttggactat tgaaaaaaac agtgaaagag aaaaaacaag
     8461 acaagtggac gaaaaatgaa aatctaacaa atgaggtgga agcgtaatgt tatatacaat
     8521 gaaagagtta ttgaaagtgg ctaaagagaa tcaatttgca gtaccagcct tcaatatttg
     8581 cagctatgat atgatgaaag caatcatcga agaagcagag agattagaag caccagtaat
     8641 cttagaaatt catcctgatg aaatcgccta tttaagtgat gagtttgttg cggcagtaag
     8701 agcctatgct tatacaagta aactgccgat cgttatccac ttggatcatg gcggtacgat
     8761 agaagatgtc ttacgggcga ttcgaaacgg ctattcttcc gtgatgatcg atgcgtcttt
     8821 gaaatcattc gacgacaatg tacagattac gaaaaaagtg gttgcgttag ctcatgcggt
     8881 aaatgtctca gtggaggctg agcttggaac gattgggaat aatggttctg ctgaaggagg
     8941 tgctgaaaca atcatttata cagatcctga tcaagcgaag atttttgtga aagaaacagg
     9001 aatcgataca cttgctgtag caattggaac agcgcatggc ttgtatccga aaaacagaac
     9061 tccaaaatta aatatcgact tactgaaaga attgaaccaa caaatcgatc ttccatttgt
     9121 tttacatggc ggttcaggta atccggacgc agaagtcagc gaagcagtaa aatacggagt
     9181 tgccaaagtg aatttgagct cagatcttaa aagcgtcttt ttcaatgctt tgagagaaat
     9241 attgagtgaa aatccatcga tgtacgagcc gaatgcaatt tatccagcag ctaataaaaa
     9301 ggtacaagaa gtggtcaaac ataaaatgga aattttgaac acagtaggca aggctagttt
     9361 atactaaaaa taaaaa
//
LOCUS       DAYYKN010000106         9281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000106 DAYYKN010000000
VERSION     DAYYKN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.107"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2564)
                     /locus_tag="ACHBB7_002196"
     CDS             complement(<1..2564)
                     /locus_tag="ACHBB7_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM9210117.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIP"
     gene            complement(2786..3670)
                     /locus_tag="ACHBB7_002197"
     CDS             complement(2786..3670)
                     /locus_tag="ACHBB7_002197"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9210118.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(3765..4202)
                     /locus_tag="ACHBB7_002198"
     CDS             complement(3765..4202)
                     /locus_tag="ACHBB7_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210119.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(4419..5330)
                     /locus_tag="ACHBB7_002199"
     CDS             complement(4419..5330)
                     /locus_tag="ACHBB7_002199"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9210120.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(5707..6345)
                     /locus_tag="ACHBB7_002200"
     CDS             complement(5707..6345)
                     /locus_tag="ACHBB7_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9210121.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(6349..8331)
                     /locus_tag="ACHBB7_002201"
                     /pseudo
     CDS             complement(6349..8331)
                     /locus_tag="ACHBB7_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            8511..9176
                     /locus_tag="ACHBB7_002202"
     CDS             8511..9176
                     /locus_tag="ACHBB7_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210122.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
BASE COUNT         2799 a         1877 c         1491 g         3114 t
ORIGIN      
        1 gggatagaaa gaacaaattt acctgtatca tcaatagttg tttcggacaa aacagcttga
       61 ttttttttca atgaaaccaa agctcctgct tcgcctgtac ctgtgattga gcgagaagct
      121 gcattgatca atgtggtact taatttagca ggctcaggag gaatcacatc gactgtcgtt
      181 ttggcagagt ccagttctgt cacttcttgg ccgcctctta cttttttagc agcaactatt
      241 tgcacttgat cccccgttgt tagtctttct tcgttgggta cttgaacctc aaacaaaccg
      301 ccttgttcgc cttctcccca cggattatat cctgtccctt cattttcatc tgtttgtcct
      361 tgtgtgagag cagtaatttc tacaggtgat ttcgtcgtat ctgcataagt aatacgtaaa
      421 gtcacttcta cttcgcctgt ccaagcatct cttggctttg cctcgacacc ttcgggaaca
      481 acgacatgcc catagatttt tttatctgca ttcgttggta ctcttaagga ttcaatcatc
      541 ggaagctggt tattggcact gattcgatta taatttttca ttccggcacc tgtctgagca
      601 aattcatttg ttaaacctgc actagggctt gatacaaaat tacctaagtc ggttccattg
      661 atcgataagt ctgtcatatc agaaaaataa tccggatctt tttcaaagtt atcgaacgtt
      721 actcctgcct ttcgccataa agcaatttct gaatgagcga tcttcaaaga agaatcgcca
      781 tttccttgaa atacccagcc acctatgcca ttagtctcgc ttccgatccg tttgttttgg
      841 aaatcgaaat aggtaggaga ttctaagtct acattcaaca catccccact gatgatgcca
      901 gtcttatctt ccccagtttt cccgaccatg actaaataag ttttttcgcc agccataaag
      961 ttcaaatcaa tatctccagt tgtatctacg gtagctccgg aatctgcgcg tatatcgata
     1021 tttgattttt ctccagtaac cgtaaaatca gctgaccctg gactttctag aaaaccagaa
     1081 gcgtcataaa attggatcgc ttggtttcct tgattgagtc tgttcacata agatttgcca
     1141 tcccctgcat ttcttacctt caatgagcct ccattagtta ctgttagttt ttgcgtaccg
     1201 ttctcaaaac gaactgcgct agaacgacca ctttgcttat caatatcgat gactgatccg
     1261 gctccttcta cgatcaaaga aagtcctttc cctacaaaac gcatcccatt gtttccaggt
     1321 tggtcgccat ctccagccat tttgaccgag atttgtccac cacttgatac atggatttga
     1381 ctagaatctc cttgcaggcg aaatccgcca gtagtcgctg tattgattgt gattttccca
     1441 tcattcgaca cagcgactct tgatcctgag cctctcattt ggataattcc gcgattgttg
     1501 tcgttggtaa tattgatctt tgtatttacc gaaaattcac ttcctgagcc actgacttta
     1561 aatgtgacat tcccgggtag cgtatcgaca gcctctcctt catcgctgac gccatctgcc
     1621 cgatcatagt caagagagac ctttgaacca ctcaagatat ggagtcctgg tgctgttttt
     1681 ccactgcccg tctcatcacc tgaatctaga aaaccataga attttcctag atatggtcca
     1741 taaagctgac taccattgat tacatttagc tgatagcttc tacaaaaata agctttaccc
     1801 ggagcggcat tcatcccttc tttcactggt tggacatcca atacaccgga aacaccatct
     1861 aatgtgacag ttgcacgagg ggcgtaaatc aggcctaatt tagaaccatc atataagtca
     1921 agtgtcccgg taaattttat ttctcccgta aaatgatatc tgtttcccca aaaatcggac
     1981 atataagcaa tgataatggc atttccatcg atatttggta tcaacccacc tggatgagct
     2041 gccgttcctt taaaattctg aaactcgatc gactgaggtg tctcattgat atagatgtga
     2101 tgtgtgccga aattatgagc aagtccgcca aaatcgataa cgatatctcg cttgatctcc
     2161 aattccctgt ctaaagtcaa aggctgagtt aaacgaatct tagctactga ttcgtctgcg
     2221 agaagctgcg tcagttcttc tgttgtggca gcttcgctgt acccatccgc tgaaacaact
     2281 ctaacctgta cctcttcact gctatcagac gtttgttcag ctacttctga ggtctcactt
     2341 gtcttatttt cttcttgcct tgtattagaa gtaggcgcct cactttggga ttctgtagtt
     2401 tcagaagtaa ttgattcttc tgaaaaagta gtcttgtcaa aactttgatt ctctgattgc
     2461 gttgattcgc ttgttttctc tacatcggca aaaacggatg tcaccatcaa gagagggaag
     2521 acaaacaaaa tcgacgcaat cagctttttt acatattgat tcattctcca ccatcctctc
     2581 tgacgttttg acaattttat tttaacgttt taatttattt gtaattgtac tttcatattt
     2641 aaaatatgta tttttatggc aaatttttct gttaggtgaa aatttctttt ttgtacaaaa
     2701 aaaggctgtg acaaaagtct tgtcacagtc ccttattccg aacaaccgat gtcctcctga
     2761 caactcttcg gtcacaacct ccattttaac aataaacctg taaatgtgag ggtaacactt
     2821 tgattgtcac aggtaattta tccccttcat ctccatcaac attcgtcgtc aattcttcct
     2881 catcagctaa tgacacaact aattgctcac aagtaagata ggctagattt ttcgtagatt
     2941 catctactcc ttttaatagg tctggcaatg ttttaagcgt atcgatcaaa gaagaatctt
     3001 ttaaataaac caaatggagc ttaccatcat ccacttttgc atttggaagt aatgtttcaa
     3061 aaccgcctac tgagttagtt agtcctatca gcaagatact gctctcaatt tcttccttct
     3121 ttccatcaac ttctacgtga aacgagtagg attgtgtgga agctaactgt ttgattcctg
     3181 acatgaaata agctagtttt ccaaatttcg tcttcttctc cgcatctaca tcattgattg
     3241 cttcagggat agagccaatt gcgacgacat tcataaaata gtcgtcattt atttttccaa
     3301 tatccaatgg cttgactgat tcaatcgaaa aatgattgat cgcttcctca ggttctagcg
     3361 gtatccctaa tgctcgagcc aagtcattca ccgtacccaa tgggaaaaat ccgaaatttg
     3421 gccgatgttc ttgttcagcg attccactga ttccttcgtt gactgttcca tctccgccca
     3481 tgacaaacac actatgatac ccttccgtag ccgcttcgcg agtaaagttt tttgcatccc
     3541 ctgctttttt tgtatgcaag acgaccactt catcaaacac cgattccaat tttgctatcg
     3601 ctaattgctc aaattccttt gcttgttcac cgcctgaact ggggtttacc actaataaaa
     3661 cttttttcat cactttcttt cccttctgat tgatttcttt aatataacga tagcataaag
     3721 aaaaaaatta ggctgtgatg aagtactgtt catgaaacat tcatttattt tttgacaaga
     3781 taaaataccc atactatgaa actgaggaaa attaaaacaa tgatatagga aataaaagaa
     3841 aatgataggc attgtatacc taataaagta aaaatcttag gtggcagata aaaacacaaa
     3901 aatagcaaga gaatgtaata gcgtttcttc cactttttgt tatcagacct tatagcgatc
     3961 agaggaatac caaatgcata actgaaaaac tcttgatgta ctgttaatat gtcacgacct
     4021 tgaataataa atgcaacaat ccccgctata attagtgtgc ctaataccaa aacggaaaat
     4081 tgatgaatat attttatata caaatgatcc attgtacttc ctgccttttt atttgtgaga
     4141 aaaacaaatc cgtatacgtg aataacttct agaaataaaa gaaatttgca aaaccactcc
     4201 atttatctac ttctacgtgc attttttaat atcgttttct ttatttaaga ttatacagaa
     4261 aaatatctag ggtggctgga ttctaagtaa aaaaaatttt taaaataaaa agaactaaat
     4321 caagtaataa ttaaactact gttgatttag ttctaataaa aagatttaga tgaacagttt
     4381 tattgattta aatagttcat attttttatt tataccgctt atttactact tgctgtacta
     4441 gaatctgaag tgaatgtact caaaacattt tcaaagtctt tgtctttgat ttttacatta
     4501 gcgtctttca atacttcgcc aattacttta ttctggaagg tactgtcact taattttgtg
     4561 tctgttgcaa tttctttcaa ttcttttttg tatttatcca tgtcgttgcc tttgttttgg
     4621 tttttcacca ttttaaccac ataatattcc gtagtataag ttgaagcatt ggtagaagtg
     4681 atcacatcag aaacttgacc atctttcaac ttaaaggctg cttctttgac ttctgccgga
     4741 accgttgtag tagtggaatc aaatttcact tttccgccat cttcttttgt cgtagtatca
     4801 gttgacttgt ctttggctaa tttagagaaa tcgtctccct tatcagcagc tttcttcaca
     4861 tcttttgcat catcttcact tgctacttgg ataatttgag cttccacttc tggatggaaa
     4921 gattcccaag ccgtttttaa atcttcatcg gtcaatttga tatgtttctt tagaccttct
     4981 tgaaaggcta gactttgttt gatactgtct ttgaatgttt gttctgtgta acctgcagat
     5041 ttcaattgtg aatcaaatga atcacccaat tgtttctttg tttgatcata ttgggaatcg
     5101 atttctttgg ttgatacgtc atctccatat ttatcttcaa atacttgata aacgatcaac
     5161 tgttgcacag cggaatctgc tccttgcatt tttttcaagc tatccattac ttcttgttcg
     5221 ctgattgtac cgcctttata actaacggct gttttttcac cggcattcgt acagcctgac
     5281 aataagccag tgcctagtac gatggcaagt gctaaaaatc tttttttcat ttatcaattc
     5341 tcctactttt cgttttttgc gaattgctaa aaacaaaggt gttcaagatg aaaatcatca
     5401 agaacacctt tactaatctg ctcgatacgt cctgaataga tataaggcta cacaatccag
     5461 aaagttcgct cagcagaata aatgtcaagt agcacatcac accttaatat actacttctt
     5521 ttgttaccgt ttttcaagtc tatcttgcgt tccgtattta acgtaactaa ttcttagaac
     5581 aaatgttatc gattttgttt ttcttcttcc gctgttggga tattcaaatc agacactttt
     5641 atgttcgatt ttccagacat ttttttgttg tttttattag aaaaagtttg ttttttcatt
     5701 tgttttctaa agcatgatct tcttgtttcc tattttggtc agatatggat catgcgtgac
     5761 cacgataact aacgtacctt gggaagcctg ttgcgataag atttcaacga cttgctcctt
     5821 atttcgttta tctaatgatc cagttggttc atcggctaaa atgattttgc tattcttcaa
     5881 taagactctc gcacaggcga ttctttgctt ctcaccacca gataatgtgt agataggcgt
     5941 acttagcttc ttgtttatat ttactttttt caatgcctca tactttgctg ttcgcttatc
     6001 tgaaacattt aaattcgaaa acttcaatcc aatatctaaa ttaaaatcga ttgtttcata
     6061 ttcaatcaac ccaaaatctt gaaaaagaaa agagagggca tttcttttta tcaagcgctt
     6121 ctctcttcca gttaaatgtt ttgtttcttg attaaataaa taaaattgcc ctgaactgaa
     6181 ttgatcattc aatcctaata tattcaataa agtacttttc cctgctccgc tctctccaac
     6241 tacagtcaca aaatccccag catccgcagt gaaacgatcc aaatgaagaa tcgttttgtt
     6301 tcgaatgatt tttttcgcac cttttatcac tatcaacggc tccaattgtt aacctccatt
     6361 catcagcaat aactgacttt tcttttctgt agacaaaata aaaacaactg aaagaataaa
     6421 atcaatgaag ctaaagaaaa aaatattaaa aagatattta ggctctcttt ctataagaaa
     6481 aaggaagatt acacttgtcg ttccccattt gatgaggttc atcatgaaga aatctttata
     6541 cgtttcaaag agcgaataac cacttgaacg aaagatagcg attttccgac aattgtagac
     6601 aaaatatatg cataacatga catagctcaa caagaagaaa gaaaagaaag tgagtaaaaa
     6661 tatctgtatc aagtaatgaa atgatctgct taacactttg acagctatct cttcagtctg
     6721 agtatacgga aatgataact gcaagttatc acgtaactga ctttcttcaa tcagctcaaa
     6781 taacctttct ttcgacaaag agcctatctt gatctttaac ggtggataga tttctccgct
     6841 gagaatattt ctttcccaat acgtactgtt tttcaatgtc agtatttcaa caaccggata
     6901 atgttcgatc caggggatag aaggaataaa tgtataaatg ggttgattgt tttttataaa
     6961 tagaaatttc ggttcttttt cactgatttc ttgataatat tctgttattt gcgccaggtc
     7021 attttttaaa tctgtatgtc tatatcgttc agggatcaat ataacagggt gcgtttcttt
     7081 ttcggttatt tggatcaacc ttccagagac atcaatcaaa ggaaatttct taagataatt
     7141 cagatttatt tttatccctc tgccaaactg ttcattttct tcattcaagt aatctgatac
     7201 attgactaat atacttccgt ttttattcaa agtttcatat aattcctgat cggctgctga
     7261 tgcaaaacga ttggaataaa taaaatccac gtggtttttc ccgatggtta ctggataaaa
     7321 tacatgatag tcctcaccag cgatttcttt tggtgtttca tgggaaacag ctaactgtga
     7381 agaagaaaaa acgatagttg ctaattcgac attggcaacg gtcaaaagaa gagaaaacaa
     7441 caatccaaat gtaaaaagaa tgtattggat atagttgaac gcttctctta acgaagcgat
     7501 tttttcaact aataaaagag aaatcaaaga gataccctat attccgctga acaagaaaac
     7561 gtacatctgg aacaaataaa gaaattgctc cttataatac ataaaaataa aaccaccaat
     7621 gattcctgca attatgaatg taataatgat tttctttttc cccaataaac accacaatac
     7681 ttgatgtaat gaaaagccat tattcaacaa taaactgatt tctttattca tcaataagaa
     7741 aaaggatgat aacaaaagga ataaaagaat caatccaata gttaaataca ctcgataatt
     7801 atatacgtct gtacttgaaa taaccgaata cgaatagcta ttttcaaagt cttcaaaaat
     7861 attagtggca taatcagtgt gaaatgcttg attgaatcgt tccacaaata gtaaaatgaa
     7921 ccgttcatag gcttcttttt ctctcgtcat caagaaaaat tcaccttcaa aatcttttat
     7981 tttgatcgat tcttcaatgg acgttacgcg aaaggaggta ttagaatagg gtaccttctc
     8041 gtgagaagat ttgacatccc cttgataata caatgtcatt tcaagctttg gctgtatctt
     8101 gaagttccaa gatgtatcta cttcattgga ttcatttact gtttttttca tataagaaag
     8161 ctcagtttct gatgagacat tttcaaatac catgattagc tgctcatatt cttcttgccc
     8221 aataaaatgt tgtggcaatc gaatcggagt ataagtatct gtttgatgga aaaattcatt
     8281 tgtccgatag agtacctgtt tatttataat aaccaaggca aaaccactca tgataccggt
     8341 cactatgatt gtgagtaaaa ctgtgaactt ttttttgatt atcaccactc tcccttactt
     8401 aaatgattct ttgataaaat cataccgttt ttcatcaaaa aaacgtatct atcaaatgta
     8461 taggtcatgt agaataaatc tatatactgt agaatcgaaa ggagaagaag atgaacatcg
     8521 gagaaaaaat acgaacttac cgcagaaatt gtaagcttac gcaaaaagaa ttaggcgaaa
     8581 aaatcggtgt ttccgataaa acaatctcta gttgggaaaa ttctcgtaca atgccagacc
     8641 ttgaaatgct gtcactttta catcagactt taggtctgcc gattgatttt ccttctattg
     8701 aatcagctac cgaatcaata gaaccaacat tgccggatga acctcttgtt atcaagaaat
     8761 cttattcatc aaaacaaccc ttctcactca aaaagacact atctttcttt ctgctcgtct
     8821 tttgtttggt ctatcttttt cttatcaatt atcaatataa aaatgaaacg gttgatcgat
     8881 tcaatccttt tttgaaacca caaatcggct ataccacact ccctgtagga tacgtggaaa
     8941 attccatcga acattgggta tgtaatgata tctttgttca tggtacatgg ctgtcttttc
     9001 acgctggaga ggtagaaaaa ggtaagaatc atgctgtaat caaacacaaa ggaaaacacg
     9061 tcatgaacgt tcgcctgctc acttgggaag ccattccctc agattaccaa gaaatcctca
     9121 tccaagacta tgacgatgcc ttaaaaagaa gccctcagga gccagatagg cattaagtag
     9181 gagatgaacg tagagataga aaaaatcaat gcacatgatc ttatcaaaaa aaaacagggg
     9241 tacaaattaa ttgaaaaagc tttgagatga aagttcctaa a
//
LOCUS       DAYYKN010000107         9080 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000107 DAYYKN010000000
VERSION     DAYYKN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.108"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..416
                     /locus_tag="ACHBB7_002203"
     CDS             60..416
                     /locus_tag="ACHBB7_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9210123.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            406..672
                     /locus_tag="ACHBB7_002204"
     CDS             406..672
                     /locus_tag="ACHBB7_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9210124.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            831..1832
                     /locus_tag="ACHBB7_002205"
     CDS             831..1832
                     /locus_tag="ACHBB7_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM9210125.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2202..2816)
                     /locus_tag="ACHBB7_002206"
                     /pseudo
     CDS             complement(2202..2816)
                     /locus_tag="ACHBB7_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3056..3301
                     /locus_tag="ACHBB7_002207"
     CDS             3056..3301
                     /locus_tag="ACHBB7_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210126.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3399..3620
                     /locus_tag="ACHBB7_002208"
     CDS             3399..3620
                     /locus_tag="ACHBB7_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210127.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            3942..4622
                     /locus_tag="ACHBB7_002209"
                     /pseudo
     CDS             3942..4622
                     /locus_tag="ACHBB7_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            4762..5301
                     /locus_tag="ACHBB7_002210"
     CDS             4762..5301
                     /locus_tag="ACHBB7_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210128.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            6321..7490
                     /locus_tag="ACHBB7_002211"
     CDS             6321..7490
                     /locus_tag="ACHBB7_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9210129.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            7963..8517
                     /locus_tag="ACHBB7_002212"
     CDS             7963..8517
                     /locus_tag="ACHBB7_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9210130.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            8573..>9080
                     /locus_tag="ACHBB7_002213"
     CDS             8573..>9080
                     /locus_tag="ACHBB7_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210131.1"
                     /translation="MKITEFSEVFKTFSNIYNRIPEIEQNVVVDNDYYIENDFNKPRK
                     YDNLYNFIHNRTTINNNSNTRVLINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAI
                     NNGNLPSNKKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIF
                     ASIHDYIGL"
BASE COUNT         3391 a         1201 c         1579 g         2909 t
ORIGIN      
        1 taattcgaat ttttatgtta tatactataa tataaaatca atatatgata taattgggta
       61 tggaaaaaat atcttttgaa acatttaaaa gacctaatgg tcatgatgaa tttttagaat
      121 ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata gaagaaacag
      181 aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta gacactaatt
      241 tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agctatctat ttccatgtgg
      301 tcaatggaag gtatgtcatt acccatggat ttactaaaaa aacggagaaa acaccaataa
      361 gtgaacttaa acatgcaaaa gaacttagag aggagtggtt cacaaatgat gattaaaaaa
      421 ttgattgaag aaaagcaaaa agacaaagat tttgcagaag catatgaccg tgaaggaaga
      481 aaactcgaaa ctgcggttgc tctttatcat gcacgggagg aagcaggcct aactcaagcg
      541 gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga gagaggggat
      601 aacgtatctt tcgataaact tgatgatatc gctagggcat taggcaaaaa gctggtagta
      661 gaatttgctt aaaagcgagt tgaaaatata cctgtaatag gctcctaact aatcaattag
      721 tttagtgggc ctttttgtac taaatagata aaagttgtcc tatagtgtaa aagtcaataa
      781 attttataca aaaattactt taaagagaca taaagagaaa ggtacaataa atgaaaacca
      841 taaatctaat aaaagtattt atagcatcac caggtgatat agcagcaaag aggaatgaag
      901 tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa gtaattttga
      961 tgccaattcg atgggaaacg aatgcgattc ctacttatat aaataatagc tctgggcaaa
     1021 gtgttattaa taaacaaatt gtagaaaata gtgatatatt attagcgatt tttggccata
     1081 agttgggttc tccagttgat aattatagat cgggaactat agcagaaatt gattactttt
     1141 ataaaaatag caaaggcgag gtaggggttt ttttcttaga tgaaccagtt ccacaagttt
     1201 tggctgaaga gtatatagaa gttaagaagt acagggactc tttagaagat aagggttttt
     1261 atggaacata tagcaaagac aatatcagaa ggtttctcac aaaaaaagtg aattatttaa
     1321 ttgagaaagc acaaaataat ttagaatcaa aacgctttga tttagattct atttcttaca
     1381 agacaccttc agtaattaac aaaaaagtta ccggaactga taaagatttt gatgacaact
     1441 tagatatttt tgatccaata gaatttgaca atgatgaaag attgctgcta atatatgcgt
     1501 ttatggatga aaattctatc ttttcaactg ataattcaac ttatagaaaa gtagttcaat
     1561 gggaaagtga taacaattta aggagttact tgagtgatag agtatttgat gtactagaaa
     1621 aattaagtaa atcgggaatt ttaaaggaag aatacgattc ttcttttgat gagtatccat
     1681 cacgctatat tttgatttca gaggactata agaaatttaa aaggtatttt ttaaaaaatg
     1741 aaaaaaagat atctgttatt atggagaaat acaaagctcc aatacaggct gatccatttt
     1801 ccaatttgga tgaatcagat ttaccgtttt aaattttaaa tagataattt cattttttag
     1861 tagcagaggt ataaactttt ttaatgtttg aattgaaaag aaatgctaat aggtcgactt
     1921 tatgtttctc ttgtgcaatc gtaaatgcgg gggaccataa aattgatcta ttatcgagtg
     1981 tgtataaaat aaaattcaaa tcttattagt ttacataaga tacattatac taagttgtca
     2041 atgttaaatg cctttaaata aggacgattt atgcatcaat tatatttatg ttaactaaat
     2101 tctttttaac ataaatgtaa tcaacttagc tagatgaatc taaaatgatt gtctgtattg
     2161 atgacaaagc taattaataa ttacgtgatt caaaaaagaa atcatacaat tagttggtcg
     2221 atttcttttt ttattcggta aattgtttgc ctagtcacat tgtagtctgt tgcaatcttt
     2281 gagatagcaa ccccattctc aagatcctga acgatactcc tatagacaag gcgacgttga
     2341 ggatctttgg tgtctgcact gtataactta ggacgacctt tatagacgcc gtttgcttta
     2401 gcgattttaa ttccttgtgc ctggcgtcgt ttcgattcag tacgttcttg ttcagcaatc
     2461 atggctgaga tttaaatgat taagtctttg atgaatttat ctaggagagg atttccaatt
     2521 gcttcagcca tgatcggtaa actggtgata ataagtcgca cattcttctt ctttagataa
     2581 ttcactgaat cgataatctc gtcataattg cgtccaagtc gatcaatggc ttctacaata
     2641 aaaatatctt gctctctgac aaaatctaaa gcttcctgga aaatcggccg atgactaatc
     2701 gtcgctccag attgtttttc gataaaaatt ttttcagcgc caaacttttt catttcttca
     2761 atttgacgtt gttcattttg atcgatggat gaaactctaa tataagcaat tttcaaattt
     2821 attccctact tttctataac gtttataata caccctaatc atacgttaga actctaaatt
     2881 tttcaatgtt atatttttgt ctatttaggg taaactctaa atagacaaac ggtttttgaa
     2941 ttctaaaagt taaatagttt ataatagaag aaaaagaatg gggtgacgat ttcttagatt
     3001 gaaaaggaaa gataaatatt atagattatt acttttgaca gctgaagcag gatggatgga
     3061 ttttccagaa gttgtaaaag aagttcaaga gataggtgca actatttctg atagccgctc
     3121 ctctcctatg aactatcagg atgcccatgg aattaaaata attgatcaag agagaaatgt
     3181 cagaaaatac acaacaataa atgagtgtgt aattaatgaa aatctttgta gggcaccgat
     3241 aacgaaacac attaagaata gaagcaaagc aaaagacgga agaactttta ctaccttcta
     3301 agtttttttc atatttgttt gctatactta gatctaagga attagacttt cgctgcccat
     3361 tatttaagtt tgaggtagcg tcttgcgagg gatcaataat ggaagaagaa aaaatatttg
     3421 aaaaacgctg ggaattggct tcagttgaac aaagagcaag gtaccataat ctaatgtcct
     3481 cttaccgtaa tattgattgg acttacaaag aaaagaagta cttgctttgg ttatgtcagt
     3541 tagatgtaaa tacctttgaa acatttgaag tgattctaga taagattaaa aacagcaatg
     3601 aaaaaagagc tgatctataa tggttcgctc tttttatttt attaactctt tggttactat
     3661 atgtaaaata cagcttcata ttacaataaa tttcaggata caaatttatt taagcatata
     3721 taaatataat tgaaatagga cataaatata tgagtatgag aaaggtgatt ccatgtttaa
     3781 taaatgggca aaccacccaa tgaactcgag aggtagagat aatttgaagg agacggacaa
     3841 gttctgggaa gaagtatata gtaaaaacgg ttctgttgca aagttttcca aaaaatctat
     3901 tttagtgtaa aattgagaaa aaagacagag aggacagagt aatgaatcat tttaaaggca
     3961 aacaattcaa aaaagacgtc attattgtcg ctgttggtta ctacctgcgt tacaatctaa
     4021 gctatcgtga agttcaggaa ttgttatatg atcgtggaat aaatgtttgt catactacga
     4081 tttatcgttg ggtataagag tacagcaaag tcctctatta tctttggaag aagaaaaata
     4141 gacaatcctt ctattcatgg aaaatggacg aaacctatat caaaattaag ggacgttggc
     4201 attatcttta tcgtgcaatt gatgcggacg gcttaacctt agatatctgg ttacgaaaga
     4261 aacgggatac gcaagcagcc tatgctttct taaaacgact ccataaacag tttggtgagc
     4321 cgaaagcaat tgtgaccgat aaagcacctt ctcttggctc cgcctttaga aagttacaga
     4381 gtgtgggttt atatactaag acagagcacc gaactgtgaa gtatcttaac aatttaatag
     4441 aacaagacca tcgacctatt aaacgacgaa ataaatttta tcaaagtctc cgtacagcct
     4501 cttccacgat taagggcatg gagaccattc gaggaatata taaaaagaac cgaagaaatg
     4561 gaacgctctt cggcttttcg gtgtctactg aaatcaaggt attaatggga ataacagcct
     4621 aagatatttg gagttcagag agggcgcgtt tgattttcaa acttcgcaac agaaccgaga
     4681 accttgggtt ttcaagtgct ttttggttca ttaatcttaa ccatagtgtt tttaaagtgg
     4741 aaaacaagca acaaaaaaag tatgtggagc cttgtaggaa tagttcttat tatttttact
     4801 gtgactgcag caacagaagc aaattattcg cataaaattc agtcgtataa aggtcgttta
     4861 gttatgacca aaaaagatgt tacacgttat acatcctcaa gaactggagg cctatatgac
     4921 tatgcaccat tagatgcatt gaaatataaa gatcatttaa agaaacatga agtaaaagtt
     4981 ggtgatcggt gggagaatgc atctcataaa gcatataatt ccaaaatagt ctatacagta
     5041 gaagatgccg atgagcaaaa tatcacatta cctgtattca attattgggg aacagttgtg
     5101 aaagtaaatg ggaaaaagag cactatagaa aatgacaatg gtttagtgtc tattaaaggt
     5161 acggggaaaa gaactatagt tgaggtttca tctacgtatc caactacgat gattctatct
     5221 ttattaatta gttcaataac ttttatattc ttatttacac gttatttaat aagatgctgc
     5281 ggtgaaatga aaatagatta gatagatatt ataaatatgt tgattctatt ttcatagcaa
     5341 tggagaaaat acattagcaa tatataaaag aaggtatcaa gagtcacagt gaatttattc
     5401 cgaaaagtga aaagagcaaa tataaaattt aatgaaaata tagaactaaa taggaaaacg
     5461 tagacgtaat tttctacgtt ttccttttca tatatattaa atattacgga aaattatata
     5521 aactaattta gtgttttgtt tgtaattcct aattttgctc cacttttaga taatttttag
     5581 gtctatttaa ctcatcagac attctatgct catttttctc caaatttgtt tcaactcttt
     5641 cgtacttaat tttgcgtatt tgaaaattct tcttttagag tacttttatt acgatactag
     5701 tatgatatat atctatagca aacagttaaa atcttcctaa aataagcgaa tagcattcct
     5761 aaaacctata aaatttttag atgaagcgac aagtggacaa ttaataaact acttgaacat
     5821 gaaattatgg atcacctttc aaatttagaa aatggaattc ttatacttat tgcgcaacat
     5881 ttattgattg caaaattatg tgacaaaaaa aactagtaga agaaagaata atggcttgat
     5941 aataaagtac tacctattaa aggtagtact ttatttaatt tagtataaat taacatacta
     6001 ctatatttta ggtttcatat tatataaatt aataatatac taccatttgt tggtagtata
     6061 atttaaaggt aataagttat tcttttatta ctatgtttgg gttttatagc aaagaaagtt
     6121 taataagagt ttggacctag caaacaatat taatgaggtc attaaatctt attatcttta
     6181 gtgatataac gaaatatcta cacagacaaa atgatagcgt aaaatatttt gtgtaaatag
     6241 atatttaggg tagaaaaaaa gaaagtccat tctgtaaaat taaagttacc acaaccgaaa
     6301 ttttatagga ggactttctc atgcccaatt ttactacaga aattatggaa acactaatca
     6361 ataaaggtga tttagatgaa ttatttcgat gtcatttaga attagctgtt aattcattat
     6421 tgcaggctga attaacagct tttcttgatt atgaaaaata tgatcgtgct gggttcaatt
     6481 caggtaattc ccgtaatggg aattattcac gttcattcag aacagagtat ggagaattaa
     6541 atttggtaat tcctagagat agaaatggta agttttctca acaaacttta cctgcttata
     6601 aaagaaccaa tgactcttta gaaaccacta ttattcagct atttcaaaaa ggaattacta
     6661 tgtctgaaat ctctgaattg attgaaaaaa tgtacgggca tcactatacg ccacaaacca
     6721 tttcaaatat gactaaaata gtttctgaag atattattgc ctttaaagaa aggtccttag
     6781 aatccagata ctctgtcatt tttatggatg ctactcacat tcctttaaaa agacaaactg
     6841 tttcaaaaga agcagtttat attgtaattg gcattcgatt agatggaacc aaagaggttc
     6901 ttgggtttag tcttgctcca actgaatctg cttatgtttg gaaagagatt cttcaagacc
     6961 taaaagaccg tggtttaaaa gaggttttat tagtcgtaac tgatggttta agtggcatta
     7021 acgatagtat tcatagtgtt tatccaaatg ctcaatttca acaatgttgt gtccatatat
     7081 ctagaaatat cgctcataag gttcgtgtta gtgatcgaca agaaatctgt agcgatttca
     7141 aattagttta tcaagcaagt tcaaaagaag aagcaaataa tcaaataagg tttatgatag
     7201 ataaatggaa aaagcagtat ccacgagtgg ttaaattgct catgaatcct gctatattaa
     7261 ccttttataa tttccctccg tcaatcagaa gaactattta ttcaactaat ttgattgaag
     7321 gttttaacaa acaattaaag aaatatacca aaagaaaaga gcaatttcct aatgaagaat
     7381 ctctggagag atttctagta tctcaattca acaactataa tcaaaagttt ctatgccgag
     7441 ttcataaagg ttttaaagaa atacatgata cattagaatc aatgttttaa cttgatcaac
     7501 agaatgggct tttccattta cacaaaatta ttgacgctcc cgacaaaatt atgatcttgc
     7561 gtaaagttat aaaatattaa taaaaatatt ttattaatga aaagcttggt agcacggtta
     7621 ttcatatgct aatttatttc actttatagt aaggggagtt cttagtatat tgttcaaact
     7681 taaatttcat ataatttaat tatgtaaatt agctattcta agaacttcta cggataactg
     7741 cccgtctaaa atttaaataa gagcttagaa tcttcatata agtaaggtta atttttttgt
     7801 acaaaaaagt cggaatattc tgagttactg aaatgatata aaatcataaa ctaaacccct
     7861 tgattttctt cacttttaac agagtatata tataagttca tgattatggt aaaatatata
     7921 tataatcatg aactcagaat atttttaaga agggaaacaa tagtgagcta taatgtacaa
     7981 ccgttaagga caaatgaaga gatacaagat tttcagtttt ggctaagaag aacaagtaat
     8041 ccagaaagag atagtttttt atttttgttt ggaatcaata atgggttacg tatgtcagat
     8101 attataggat taaaagttgg agacatacga ggtaaaagta agcctattat tgttgaacat
     8161 aaaacaggta agcgaaaacc gatttttata gataatttga gagaagagat tttattatat
     8221 actgaaggta aagaagaaaa tgattggtta tttccaagtc ggcaacaagg tcgacatata
     8281 acaagggatc gtgtctatca gatatatgca gatatagcag aaaaattagg aagagatgat
     8341 ataggaacac atacattaag aaaaactttt gggtatcact actacaagaa aactagagac
     8401 attgctacct taatgtttat ttttaatcat agctcgcaag caattacaaa aaggtacatt
     8461 ggtataactg aagatgaaat aggggcatcg ttacgtggtt ttaaattggg aggctaattg
     8521 agatattagg atatattttt tgtgtataat gaaaacttag aaggtgagat taatgaagat
     8581 aactgaattt tcggaagtat ttaaaacgtt tagtaacatt tacaatagaa taccagagat
     8641 agaacaaaat gttgtagttg ataatgatta ttatatagag aatgatttta ataaaccaag
     8701 aaaatatgac aacctataca attttataca caatagaaca acaataaata acaacagtaa
     8761 cactcgagtt ttgattaatt cagcatattt tcatattaaa tatttaaaga aatatgagta
     8821 tgaagaagtt gtattagatt atgggctaaa tagacagtca tttttaattt atgcaataaa
     8881 taacggaaat ttgccaagca ataaaaagaa cataattatt gaaacatatg cctatctgga
     8941 ggggggatct aaaaaactaa ttaagtcatg taatatagag tatcaattat cagtaggaga
     9001 aattaaatgt gttttcgaag aagatttaga acttgactta aaaaaaattt ttgccagcat
     9061 tcatgattat ataggtttga
//
LOCUS       DAYYKN010000108         8951 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000108 DAYYKN010000000
VERSION     DAYYKN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.109"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..886
                     /gene="purR"
                     /locus_tag="ACHBB7_002214"
     CDS             68..886
                     /gene="purR"
                     /locus_tag="ACHBB7_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM9210132.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1389..2762
                     /gene="glmU"
                     /locus_tag="ACHBB7_002215"
     CDS             1389..2762
                     /gene="glmU"
                     /locus_tag="ACHBB7_002215"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM9210133.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2856..3827
                     /locus_tag="ACHBB7_002216"
     CDS             2856..3827
                     /locus_tag="ACHBB7_002216"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9210134.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3833..3935
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4001..4570
                     /gene="thiT"
                     /locus_tag="ACHBB7_002217"
     CDS             4001..4570
                     /gene="thiT"
                     /locus_tag="ACHBB7_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM9210135.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4651..5424)
                     /locus_tag="ACHBB7_002218"
     CDS             complement(4651..5424)
                     /locus_tag="ACHBB7_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210136.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5691..7391
                     /locus_tag="ACHBB7_002219"
     CDS             5691..7391
                     /locus_tag="ACHBB7_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM9210137.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7361..7579
                     /locus_tag="ACHBB7_002220"
     CDS             7361..7579
                     /locus_tag="ACHBB7_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM9210138.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7599..8489
                     /locus_tag="ACHBB7_002221"
     CDS             7599..8489
                     /locus_tag="ACHBB7_002221"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM9210139.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8507..8791
                     /locus_tag="ACHBB7_002222"
     CDS             8507..8791
                     /locus_tag="ACHBB7_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210140.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3020 a         1532 c         1886 g         2513 t
ORIGIN      
        1 ctcgaatttt tgaagaaaaa cttatataat gtaaaacgat aaaatttctt aaaaggagtt
       61 ggaataggtg aaagtacgtc gcagtgaacg tctagttgac atgacacagt atttattgaa
      121 tcacccgcat gaattgattt ccttaacaag ctttgccgaa cgttatgaat ctgctaaatc
      181 ttctatcagt gaagatttgg gaatcatcaa gaaaacattt aaagaacgag ggtatggaac
      241 cttggaaaca atcccgggtg ctgcaggtgg tgtattgttc atccctgaaa tttcatatga
      301 agaggcgaaa aaatatattg aatctctagc agaacgtcta tcagaacaag accgtctgct
      361 tccaggagga tatgtttatc tctctgattt gttaggagaa cctgaactgc tgcgtcaagt
      421 tggaagaatc attgcttcta aataccttgg tgaaaaaatc gatgctgtaa tgactgtagc
      481 cacaaaaggc gtacctattg cccaagctgt gtcctattat ctgaatgcgc cattcgttat
      541 tgtacgccgg gactcaaaaa taacagaagg atcaacagta agtgtcaact atgtgtctgg
      601 atcttctgaa agaattgaaa agatggaact gtctaagaga agtttgaaac gtggttcacg
      661 tgtccttgta gtggatgatt tcatgaaagg cggcggaaca gtcaacggaa tgaagagctt
      721 gattgaagaa ttcgaatcag aacttgttgg catcactgta tttgccgaat caaaattcaa
      781 cggtcatcgc gccatcgatg attatacgtc actgctttat gtcaaagatg tagatactca
      841 aacgaaaaat attacggttg tgccaggaaa ttatttttca gaataagagg ttgtgaaaaa
      901 gctgcttagt ttcgagtagt aagaaaaatt ttgtgaaaat ggctttccac attttttcat
      961 aaatttggct taataccgag aaactggctt ttgaacaccg tttatctaga ggttgtgaaa
     1021 tcagcgtcct gtcctgagta ttagctcaat aaacggaaaa attgctcctc atattttgcc
     1081 gtttattgag ctaataccga aggtcagctg attgaaaaac gtttaatgaa gaggttgtaa
     1141 gcctaccgtt taactctaag tcacaagaaa cgatttcaaa aaacagcttt tcatgtttta
     1201 tgagtagtgt gacttgtgac cgaggagttg gcaggagagc accggttacc aaatagatag
     1261 gcccgttatg gtctatctat ttttttatcc agaaaacgct gggataaaag aaaatcactt
     1321 gttttgttga aaaaatgggt atactgggat aaactgaaat cagcaaacta tatttaggag
     1381 tgacgcagtt ggacgcacgt tatgcaatta ttttggcggc agggaaaggt acccgcatga
     1441 agtcaaaatt atataaagtt ttacatcccg tttctggtaa acctatggta gaacatatca
     1501 ttaatcgggt cagtgaaacg aaaccagatg aagtgattac gatcgttgga cacggagcag
     1561 aacaagttaa agcacaacta ggagaacgca gtaaatatgc gttacaagca gaacaattgg
     1621 gaacaggaca tgctgttttg caagctgctt ctttcttgga aggaaaaaaa ggaacaacgt
     1681 tagttatcag tggcgataca ccattattga ctactgaaac gctgaacaat ctttttgaat
     1741 atcaccaagg aaaaaatgca agtgcaacga tcttgacggc acaagcagaa aacccaacag
     1801 gatatggacg aatcatccgt gaccatattg ggatcgtaga aaaaatcgtg gaacaaaaag
     1861 atgcaacacc agaagaagct cttgtacaag aaatcaatac aggaacttac tgttttgaca
     1921 acgaagcttt gttcgatgcg ttaagcaaag ttggaacaaa caatgctcaa ggtgaatact
     1981 atctaacaga tatcatcgaa atcttaaaag aagaaggcca tacagttgct gcttatcaaa
     2041 cggatgattt tgaagaatca atgggagtca atgatcggat cgcgttagct aaagcaaatg
     2101 agatcatgag aaagcgaatt aaccagatgc atatggtcaa tggcgtaagc tttgtcgatt
     2161 cagcaacgac atacatcgat gcaggcgtag aaatcggtcc agacacattg atcgaagcag
     2221 gtgtacaaat ccaaggaaat acagtcattg gttctgattg tgtcatcggg tcacattcaa
     2281 aaatcgtaga cagtaggatt gaggatcacg tcgtcattga aaactcagtc attgaaagca
     2341 gccatgtcaa gaaacatgca gatgtgggac cttatgcaca tttacgacca aaagctgaaa
     2401 ttggcgaaaa cgtccatatc ggaaactttg tggaagtgaa aaatgctcaa atcggcaaag
     2461 gaactaaagt cggtcacttg acttatgttg gtgatgctac gctgggagaa gaaatcaatg
     2521 tcggatgtgg tgttgtcttc gtcaactatg atggaaagaa caaacatcat acgactgtcg
     2581 gcgaccacag ctttattggt tctagcacga atatcatcgg acctgtcgag gtagctaaaa
     2641 acagttcgat tgctgccggg tcaacaatta cagacaatat tccagaatat gcgctggcaa
     2701 ttgcgagagc aagacaagtc aataaagaag gatatgcaaa aaaactccct tatctaaact
     2761 aagacttttg caaaatcact tgatttattt aaaaaatatg actactattt attaggtaga
     2821 attctagagt tgtgaaaaag aaagtggagg tccccatgtc aaaacattac tttgacccaa
     2881 gattaaaaat ttttgcgtta aattccaatc gcccattagc agagaaaatt gcagcagcag
     2941 tcggcgtcga gttaggaaaa tcatctgtta ctcaatttag tgatggagaa attcaagtaa
     3001 acattgaaga aagtattcgt ggatcgcatg tctatgttat tcaatcaacg agcagcccag
     3061 tcaatgataa cttgatggag ttgctgatca tgattgacgc actgaaacga gcaagtgcaa
     3121 aaacaatcaa cgtagtcatg ccttattatg gctatgcgcg tcaagaccgt aaagcacgtg
     3181 cacgggaacc aatcacagcg aaattagtag caaacatgat tcaaaaagct ggtgcaacaa
     3241 gaatgctgac tttggacctt catgcagttc aaatccaagg tttctttgat attccagtcg
     3301 atcatttgat gggtgcgcca cttattgcca attatttctt agaaaaagga atcaaaggcg
     3361 atgatgttgt cgttgtttca ccagaccatg gtggtgtcac acgggcacgt aaattagcgg
     3421 aatttttgaa atctccgatt gcgatcatag acaaacgccg tccaaaagca aatgttgcag
     3481 aagtaatgaa tatcattgga caagtcaaag gcaaaacttg tgtactgatc gatgatatga
     3541 tcgatacagc aggtacgatc acattggctg ccaatgcttt gaaagaagct ggtgcaacta
     3601 gtgtgtatgc ttcatgtaca catccagtat tatccggccc tgctttacaa agaatagaag
     3661 attcagctat cgagcgtcta gttgttacag actcgatcta tcttccagaa gatcgtaaaa
     3721 tcgacaaaat cgatgaagta agtgttggcg gactaatggg agatgcaatc aaacgtatcc
     3781 atgaaaacaa accagttagt ccattgtttg aaacaaaaaa taaataataa ataaactcac
     3841 aaaggggagt ccaattgggc tgagattgaa tctatttcta aacccttcgt acctgtatcg
     3901 gttatgcgag cgtaggaatt gtgaataaac agctgtttga ctattttcga aagctgcttc
     3961 ctcctttgtg acttttttat tcaaaggagg aagttttttt atgggaaaaa atagagtttg
     4021 ggtagaagga acgattgttg cagcgttagc gatggtgttg tcgttgattc cgatccagat
     4081 cggcagcagt ttttctattt cattagggca gattccattg actctttttg cgttaagaag
     4141 agggacaaag gcagggatgc tggcaggttt catttggggc atcctacatt ttccgttggg
     4201 acaagtttat tatttaagtg tcgttcaagt gttgattgag tacccgattg cctatacttt
     4261 tgcaggcgca gcagggctca tggcttcaaa tgtccaaaaa ggctatgtag acgataatac
     4321 gaaacaaatt agaaaaagca tcgtgtttgg tgcattgatt ggcgcacttt ccagatattt
     4381 ttggcatttt attgcaggag tggttttctg gggagcgtat gcactatggg ggatgaatcc
     4441 ttggctcttt tctttcgtaa tgaatggtgc cagcgggttg agtacagcta ttgtaacgat
     4501 catcgtttta ttagggatcc aacaaacgac tccttcgatt tttgtgccag ataaacgaca
     4561 ggagctgtaa ataaaaaaga agcaagaaac ttgagacaga atcatgtcct agtttcttgt
     4621 ttccttttta ttttttgata agaaatagat ctacttcaat ggcagataag aaagatcggc
     4681 atcttctacc aattgccagt gtcgatcggt gacagagtag attaaagtag cgatgatttc
     4741 ctcttctcga taccgggtgc cttttaattt ccaaatataa tcttcaatca catagattgg
     4801 gatacgatat ttaccatcga tcaaaccaac ttgatcaaaa gaaaaaatga tcccttcctg
     4861 catcaaatta cgcatttctt tcaataattc cacactgatt ttttcaacag gtgtttcttt
     4921 tttgaaaaaa gattttttta cattgatctt atctgacaag atcgaactta atttactagc
     4981 aaaaatccta taaaattcat ctaaatagcg ataataaatc cgattcagct gatcattaag
     5041 atcgaagtat acaaaattat tctgtagttt ataaaaaaac ggcgaatgaa gatgtgtttt
     5101 catatgacca aaatataata attctgaaat ttctaaaggt gtcaattctt tcagcatcgt
     5161 taaatcagta aagtcgatcc acttcaattc atcgaatcca cgcctgttac gagaagagga
     5221 aaagaaacgc tgaatatttt ctgtaccacg gattactttc agccctgtat gggattcata
     5281 ttctccagca gaagcagatg gatcaagcaa gagcaagttt tgaggaaaat gcacgatact
     5341 gtttgcaaag tcagtagcag tcagcccttt tgttaaaaca gcattacttg tggtatcaat
     5401 atgtacataa actgactcag tcatcatgtt cctcctctta tttcctctta atctctttta
     5461 ttttacaaca tttgcattaa aaaagcttgt ttcttttatt ttaaaattcc attacaaaat
     5521 cacgttttca ttgaaaacac tctcattttc aattaatttt tgtgattttt gtattttttt
     5581 atttaaagga ggttttatga attgttttct tatggaatct acaaagtcga atacgagact
     5641 catgaccttt ttatgacgta ctggtatact tgaagaaaaa ggagagcgcg atggagataa
     5701 aaaaagttta cttttcacag aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac
     5761 cccttccatt ttttcaaagt catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa
     5821 agcaatgtgc ttttaaagca gtatcgaaaa aatataccgt ctattttact attgctaaaa
     5881 gtagaggaga tgttattttt aataatctgt tgattgaagg tgaaactttt gaatggaaaa
     5941 aattttttcg gtatatggaa gcttgcgctc gtttttttat taaagaaaac tgttgttttt
     6001 tatttcaatc tcctctatca gaagaatggt tgcgtatttt tcataaaaac ggctatcaag
     6061 gaaccacaca attaaataaa aagctggtct atcatacagc acttgtttta ggtggaggag
     6121 gagcgcacgg tgcttatcag attggtgtgt ggcaggcgct gaaggaacat gatatcaact
     6181 ttgagattat cacaggtacc tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag
     6241 atatgaaaaa agctgtgaat ttatggaaaa agctgtcgac aagacaagtg cttgcgttac
     6301 cagaaatggc tgcagttgaa gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga
     6361 cgaaaacagc actaattgaa ggcggggttt cttctgctcc attggaaaag atggtcaaag
     6421 acaatttgga tttgtcctta ctcaaacata atcccaaaat acgtttatat acggtgtcta
     6481 ctaaactgcc agaactagcg gaagtagtga ctgatatcca acagacaaaa acagaagaaa
     6541 taccagattg gatactcgcc tctgcttcat tttatcccgc tatggcttat cgaaagatcg
     6601 gaaaaaataa atatgcagac ggtgggtatc gcaacaaaat acctattgat attgcgatta
     6661 acaaaggagc aacagaggca tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa
     6721 ttcgtatgcc agacattttt atccattgga aatgtcaaac attgtggaca ctaggcagct
     6781 ttctgctttt tgatagccaa aggaaccaat taaatttaca attaggctat ctggaaatga
     6841 aaaaacgatt aggctattac tatgggaact ggtatacatt tcattctgtt aaacctgcaa
     6901 gaacatactg gcgaggattt ttgtcttact taacaaaaga aattcagtta gaattatctt
     6961 ttttcaaaag cataaaattt tggcaaaaac tgcgtaattt atataagaac agggtcgtac
     7021 cagagacatg cggattggcg atgctagaac ttttagcaaa aaaacatttt ttactgccca
     7081 atgaaatcta tcaagtagac atgatgattc aaatgatctg ccaacaagat ataaaaagta
     7141 tgccggatga tttattggca caaattggtc aattaagtac tgaagaatgg cgaaggtatc
     7201 gaaaatacca gcaaaaaata caaaatgagc gcacgaaaac tgtttatttt gattatctgc
     7261 tacaaggaaa acgcaaggat cgtttgcagc atgaattgct taagcaacca gttgacacat
     7321 tactgatcct atatttatat tatctcaagg aggaacaacc atggcacaag aattttcata
     7381 tgaaatcgta gaagaaatcg ctatattgtc agaaaacaac aaggggtggc gtaaagaact
     7441 gaacctagtc agctggaatg gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga
     7501 ccatgaaaaa atggggaaag gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa
     7561 agcaatagaa aatatgtaat gaggaaggac cctgtcgaat gaaaagtatg actggttttg
     7621 gcaaagccat ccgcgaaaca aaagcgtatc agttagatat tgaaataaaa agtgtcaacc
     7681 agcgttttct agatgttcaa atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt
     7741 ttcgtcagct tattaaacag cagttaagcc gcggtcgaat cgaagtattt gtgaatctag
     7801 cttatattgg caaaaacgaa aaacagatcg tcatcaactg ggaattgatt gattcactta
     7861 tcactcaatt gacagacgga attcaacaac gatacggtag tcaaaatcaa gtgtcaatta
     7921 gtcggatggc ggaacgcttc gcgctggacg aaaacttcgt aacggtagaa gaaaaaccgg
     7981 aagagagcat ggaagaattg gcacagttag ctctagatac attgaaaagc gcacttgatt
     8041 ctattgaggc gagtcgagaa aaagaaggtc aagctttagc tttagtgttg aaaaagaata
     8101 ctgaagaatt caaagaagta ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg
     8161 aatttcaaat aaggtatcaa aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc
     8221 aacggttgtt gacggagcta gccatcttgt tggaacgcgg agatattcac gaggaactgg
     8281 atcgtctggc gatccatgta caaaaactag atgaacttat cgaagcacaa tgtcctgtag
     8341 gtcgggaatt agatttcctg atccaagaaa tgaatcggga aatcaacacg atcggttcca
     8401 aatcaaatgc aatcgaaatt aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa
     8461 tcagagaaca agtacaaaat attgaatgac gaaagaggta gagataatga aatattatga
     8521 cgtaacgttc cacgaattaa gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa
     8581 aaacggcttt gacgtatgga aagatgcctg tgcagactat aatgagaacg aactgtttat
     8641 tttgatcaac gacggtgctt acgtgacgat gaatcggaaa tttattgttc gaattgacac
     8701 agaagaggta gaagatccga cagaaaaagc acgttctcga aaagatgaga tcatgggtgt
     8761 agtgaatact ttgtccaata tgggctttta gcaaggatgc tgaacaaaga aatacaataa
     8821 ataatcagaa ttggatttct tcgcttagct tctttgctct aggaatacat ttaaaggttg
     8881 tgacaaaatt cgtgtcacaa tcttttttta ttttgaataa gtgatgttca aaaaccattt
     8941 tctcggtatt a
//
LOCUS       DAYYKN010000109         8866 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000109 DAYYKN010000000
VERSION     DAYYKN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.110"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            646..1119
                     /locus_tag="ACHBB7_002223"
     CDS             646..1119
                     /locus_tag="ACHBB7_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM9210141.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1128..1355
                     /locus_tag="ACHBB7_002224"
     CDS             1128..1355
                     /locus_tag="ACHBB7_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210142.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1991..2362
                     /locus_tag="ACHBB7_002225"
     CDS             1991..2362
                     /locus_tag="ACHBB7_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210143.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2618..2860)
                     /locus_tag="ACHBB7_002226"
     CDS             complement(2618..2860)
                     /locus_tag="ACHBB7_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9210144.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2892..3794)
                     /locus_tag="ACHBB7_002227"
     CDS             complement(2892..3794)
                     /locus_tag="ACHBB7_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9210145.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3807..3995)
                     /locus_tag="ACHBB7_002228"
     CDS             complement(3807..3995)
                     /locus_tag="ACHBB7_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM9210146.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4009..4572)
                     /gene="amaP"
                     /locus_tag="ACHBB7_002229"
     CDS             complement(4009..4572)
                     /gene="amaP"
                     /locus_tag="ACHBB7_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM9210147.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4610..5503)
                     /locus_tag="ACHBB7_002230"
     CDS             complement(4610..5503)
                     /locus_tag="ACHBB7_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9210148.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5581..6504)
                     /locus_tag="ACHBB7_002231"
     CDS             complement(5581..6504)
                     /locus_tag="ACHBB7_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9210149.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6553..6903)
                     /locus_tag="ACHBB7_002232"
     CDS             complement(6553..6903)
                     /locus_tag="ACHBB7_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9210150.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6936..7838)
                     /locus_tag="ACHBB7_002233"
     CDS             complement(6936..7838)
                     /locus_tag="ACHBB7_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM9210151.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7831..8688)
                     /locus_tag="ACHBB7_002234"
     CDS             complement(7831..8688)
                     /locus_tag="ACHBB7_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM9210152.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2473 a         1699 c         1469 g         3225 t
ORIGIN      
        1 aaataaacat gtgccatcta tctgttggca catgttttag gatttatggt actttttatt
       61 cggcaatcca ttttattttt ctacttcttc ttaatacctc taacactttt gatttataaa
      121 ctaatccaat gcatctactt gcgcttatta agctatttca gctagatagt accaccccat
      181 gcgtcaatgc ttgattttcc ttttgtataa cattgtataa gcaataaagg acaaataagt
      241 ggtgattaca ccaaaccatt gaataagaaa aattctgata cgtaaataaa catgaacttg
      301 aaaaaattaa tgaatcggtt agctactacg tagttgtctt ttttctcata cagttctgtt
      361 aacttacact tctcttcgct agataataaa aaatcctcca actgtattgt ccaatataat
      421 cccaggcatc taaactagca ttatagaaga gacacacata gaatctaaaa taactggttt
      481 taaatgtttc ttacaatcaa aaaaagctgt taaatgcact ccacaccttc atactctatc
      541 attttttata aacgatatat ttttattgtt tttcgataaa aaatagttgt tttacaataa
      601 ttattctgct atattttagc tatcaaagat aaaagaggga taaaaatgaa atttaaaaca
      661 ttgctattaa aatttgtcgt tttaggtata agtgccgtat ttattttgtt cggcgtactt
      721 ttttatactc agatcgctac aagcgaaaaa ggattcaatt ttgattggca gaccacatta
      781 tttgtttctg tcatttactt tgcccttctc ctagggatcg ttattgctta tttcttattg
      841 aaactattga aaaaaatcga agataaccaa gctttttcca ctcaaagctt aaacatacta
      901 aaaaaaatcg aacgaaccat tttgtttatt ggtttcgcct tttttggctt acttcctaaa
      961 tttatgaaag tagcggattt agacgatgct ccaggtttga tgttggtagg aatcgctgtc
     1021 gtattcattc cttttgcagt atacacatta acaagcgtgc tggaaaaact gcttgaacat
     1081 gcgattttac ttaagacaga tcatgatttg acagtgtgag gtgcgacatg gcaattatcg
     1141 ttaatttaga tgtagtatta gctcaaaaaa aaatgagtgt gactgaattg agtgagaaag
     1201 tgggcattac gatggcaaac atctctattc taaaaaatgg aaaagcaaaa gccatccgtt
     1261 tttccactct cgaaaaaatc tgccaagttt tggagtgtca accaggtgat ttgttgacct
     1321 ataaaaatga ctcgaatact ataccagaag aataaaataa agtagaacaa acagtaaata
     1381 aaaaataatc tgaatgatag atgtgtgaga cttttagaag ttatttacgt ctattattca
     1441 gattttttat ttcgattgaa taccgtttta tctataaata agatatgtac tgactcatac
     1501 aaagggggcc ttttcttctc catgtactat cacttttcct gatacaactg gtttcctgtt
     1561 acgttgtagt tggatctgct tttagtataa tactttattc attccaccag actgatagca
     1621 gtttgagcag tctaaataat catgtagtat tacaggccat tctgtttcat accttgaagt
     1681 gcttaaaact tcagctaatg gcccattgtg ttaactctta ctccattctt ttttagtaag
     1741 ctcccatata tactgtaaag ccttctatat attatgtcat gttgcttttt aacatttact
     1801 atgtagtttt gataaaatga tttgaataac agtgatggac aattcttttt tatttttaac
     1861 attattttga taataatgat tatttattta aaaatacatt agataacatt tgttacgaca
     1921 cgaaaaagct tcacaaacaa atatcgttat tttaaaatga gggcgaactc acacaattag
     1981 gaggaatagt atgccattta ttgtttactt ttttctttca ttactcgtga tctatattcc
     2041 attaccaaca tttttcctgc tcgtcaatcg tctagcagag caacatgata cgattgctgc
     2101 tcttacgaaa ctttgtttac ttctatttgt ttgtctagat tactttacga gctattattg
     2161 gctaaaacag acaagagtaa aaaagagata ttatttcttc cttgtctttt tcaatctctt
     2221 tgagtttctg atccattgct ttttacttca tcagtatcat acgatccatt tgtggatcat
     2281 ctgggttttc aaagtggtcg tattattttg tatgttcacc tttcctatct caagaaaatt
     2341 tagggaggca ttgttttatt aaagaagctt ctctgtatca ataaagacac atttaaggaa
     2401 aaacttttaa aaatctaatt gggaatttgc ccgcataaat aagaggctgt gacaaaagtc
     2461 ttgtcacagc ctcttattcc gtataaacag tgttcaaaag cttgcatctt cgataatatg
     2521 cccaaccaaa tgaaaaatat gagaagctat ttttgtttgg ctgttcttat tacttgatga
     2581 agaacagctt tttcacgacc tctttttaaa agtcttttta cagttttcga gcaaagaatg
     2641 atacgattag tactaaaatg atggcaccaa tcaatgatgg cacgattgcc attcctgcaa
     2701 tacttggtcc ccaagaacct aataatgctt gaccaagtgc tgatccaact aaacctgcta
     2761 cgacatttgc aataatcccc atcgaagaac ctttattcgt gattgctcct gcaattgctc
     2821 cgataattcc tccgacgata agtgaccaaa taaatcccat gtttcgttcc tcctaatgtt
     2881 ttacttcctt attactgaac ccgtggttct gtattttctt ttactttttg tgcgccgctt
     2941 ccagctgctg attttacttt atctgtttga ttcgaagcaa attcaccggt ttttttcgct
     3001 gcgtcagtta ctttgtcttg caccgtttct tgatcttttt caaattcttc ttttgttttg
     3061 atgtccacta cgttagcgtt gacttcgatc acttctaaat gtgtcatatt ttttacttct
     3121 ttgccgatta cttcttgcat ttcttcaaag attttttcga tatctttgcc atattccaca
     3181 atgacatcca tatcaactgc tacttgtttt ttaccgactt ctgtttcgat cccagccgtt
     3241 acattatctg tattcacaag ttttcctgcc acattagaga agaaaccacc gtttgcagat
     3301 aataaaccgt ctacttgttc aattgcgatt ccgatgattt tttgcacaac tttatcttca
     3361 taattcagct caccgcgcat tgaatcttcc tgttcagaat catgctttcc ttggttattt
     3421 gtatgatcag aaccaagttt tccttgtccg tttgtgtggt cagaactaag tttttcttgt
     3481 gctttcgcat ggtcagaagg atgtttttgc tcttccacag gtttagaagg atcgattgca
     3541 tcatgttgtt ctggaacttt agattctgga gttcctggat gatgaggtgc atgcttttct
     3601 gcatcatgtt tcttctcttc tactccttgt tgaacgttag ggactactgg atctggatgt
     3661 gcagatactg gtgctgtttt gttcttttca tcgttcacac catttgattg gtttggtgtc
     3721 gttgggtctg gatgtgcaga cactggtttt tggttgttca ttttattgtt attatttttg
     3781 ttattgttat tcatggatgt ttcctcctaa gatttaaaat agtgatctaa gattttcgtt
     3841 tgtttcagat aaaaaccgat agcgacgccg atcgctgcca tgatcaagat caataaggtc
     3901 ttgaaaaagc ccacggtgat ccataaaacg gctaatatca atccaagtac cccaaagatg
     3961 atagggagct tgtatgtcga aaatagttct ttcatttttg gtccccccct attctactcg
     4021 tttgcggtca ttgcttcgtt tagctggttc ttcaaagcct gtgtatttca ccgatacttt
     4081 cactggctct tttgttccca atatgtgttg tacttcattt tcgatttctt tcatcaatgt
     4141 tcctgtttta ccaatgagtg ctgaagttcg tttcaactcc ccttttacat tgacggcgat
     4201 cttgtttttc gttgctttga catttacttt tggtgaatgg atgaattcat cttcatgcaa
     4261 gtgagaacga accattcctt caattgcttt tttatccaac gtcaactccc catcaccatg
     4321 tttcagtaga aagtttccga ttgtttttgg ataaaacagg aaaacaagta acgtgataat
     4381 tgtcaaaata gcaaatgcaa cggcaagcca aaatacatat tgttgcacgt atgaattagt
     4441 gatggaaaag aatcgaaact tctctagctg aaaaggcaat ggataggttg cacttaatag
     4501 tatcgcaaac aaaagcacag atagcaaaag taatgttccg atcactccga tcgtttttac
     4561 acctttattc attttcgcac ctcctcatag gggtgaaacg atcaacttct tataagttga
     4621 tcgcttctcc accagttact ccgtaaattt gggctgtaac ataacttgct ttattcgatg
     4681 ctaagaaaac ataaactggt gctaattctc ctggctgacc tgcacgttct agtaaacttt
     4741 gctgaccaaa ttcagggatc gaatcagaag gttgtccttc gtctagttgt aatggtgtcc
     4801 agattggacc aggtgctaca ccatttacac ggattccttt aggtgcaagc tgttttgcta
     4861 aaccgatcgt aaagttagcg attgaagctt ttgttgctgc gtaatctaat aaatgttcgc
     4921 ttggattgaa tgcttgaaca gaagttgttg ttacgattgc tcctcctgct gggatatgcg
     4981 gaacagcttc tttcactaaa gcaaacatag cgatgatgtt tatcataaat gtgtctttga
     5041 cttgctcaag tggtaactca gcaatagact gacaagaaat ttgctgtcct gcgtttagta
     5101 ccatcgtatc tagcccgcca agttcactca cggctttatc tatgatttct ttaggtgctt
     5161 gttcgtcacg aagatcaaaa ggtagtaaga caatcttacg tcctgcaact ttcacgtatt
     5221 ttgctacttc ctctgcatct ttttcttctc ctgggaagaa ttgaagagcg atatctgccc
     5281 cttctctagc aaatgcaatc gctgctgcac gaccgatccc tgaatctcca ccagtgatta
     5341 gtacacggcg gttttctagt tgattattgc cttcatagga ttcttctcca caatcaggaa
     5401 caggaatcat attctgctgt aaagctggtg tatcctgatc ttgtttagga aatttctccg
     5461 tatggtacaa gtttcgcggg tcgattaatt ttggttctgt cattgctgta ttcctcctag
     5521 tttttaaatt gtattgccaa aacaaatttc tttgtttgat acctgtttta aatagattct
     5581 ttaatttgta taatctttcc ggtataaatg gtaacctaaa actactgctg ctatcaaaga
     5641 gccaaagact acgagtataa agcctagagc agaactttta cccggtaatc ctcctgtatt
     5701 cattccccaa atccctgcga acaacgccgg aacggaaatg attaatgcag ctgaatctag
     5761 atatttcatg atatgattta gattattgtc catcatcccg ttgaatagat caccaatact
     5821 ctctaataaa tctcgataga tctcgatcat tttttctgtt tgtcgctgtc tcaaatggac
     5881 atcatagagt agctgttcat cgtctaaacg tttgatgaaa gtttcagaat tccataattg
     5941 atccattgtt tcccgttgat ctcttaaggt atgatcgata tatacgatgt ctctttcaag
     6001 atctgcctgt ttatacagtt cttcattttc cgttgtttta cgagctgctt tatccaaagc
     6061 gtcaattcgc tttttcatct ctcttagttc tttcacgtaa tgggtgtaaa tatctaataa
     6121 tgtatacgtg atgatctttt caaacgagtt taaatgattt ccatgctttg tcagcagccg
     6181 atccacaaat tcggtttttc tatcgataca agtaatcaag agattatcgg aaagtacaaa
     6241 agatattggc gttaattttt tttcgatacg attatctgac tgatctagat taatcactac
     6301 caaagaaaat ggatgctcta gtttcgtctc ataaaggcgc tccatccgag aaacttcttc
     6361 cggatattcg gttcctacga aaatatcatc aggaagctcg aattcttcga ttgcttgctc
     6421 aatctcttcg ggctttgcac tatcaagtac gagccaatta tatttttttt catctgtttc
     6481 tattacattc ttttcttcat ccacaaagta gtatctcaat gtatacctcc tttttggact
     6541 ttctcgaaac tattagctga agaagcagtt ctttaatttg atattataat cgagtactgc
     6601 gatttcattt tctaatgaaa agctgtcaag ctgtttgttg aagtccatgt tcaatcgttt
     6661 accgaaataa ttttgctcta atttagatgt atagatgtcc aataatggat gctcgatttt
     6721 agtcgcatat tctccaaatt ccgaaacaaa aggaatgatt tgaaaaaagc tgccttttgc
     6781 aaaagcacct ttgtccctta gaaatggatc ggaatcattg ataagcgcga gtgcgatttt
     6841 tgtattttcc ccaacctttt caattaacgt ctctactgca tttttagaca ttgtaatttc
     6901 cattcctact ccccctcttc agttgttgtc ttctattatt taacaactac atctgttttt
     6961 ggttgaactt ttgcatattt tggatattta gggaatttag ctacacaacg gaaagccgtc
     7021 atccctgcat atttttcgta atatttcttc gttggccctt caaaatcctt gctccacggt
     7081 ttcacatgtc ctgaaaaatg gataatgtaa ggattgttgc gagtttcttc gtattcacgt
     7141 tcacctgctg ctgttggatg tttttttgct tttgccatga tatagccttg tgcattccaa
     7201 cgcggatgaa gtggtaacca acggtcatgt aagatagcat ttaatgcatc ttggtcatgg
     7261 aatcgtaatt tttctggatt atgttcgatg aaatctaata ctttttgcgt aatattttca
     7321 tctaaccatt ttttcacatt gatcagcatc aaacctgagt tgaaataacg cattgattta
     7381 gctgaaattt tcattttttc cagtcgttga tggaacccag cgtcttcaac agctgctacg
     7441 attgaatcac cgaagtcaag acgccataat ttagagatat cttgtaaagc gatcatgtcg
     7501 caatccatat ataagatacg ttcaacttct gtgccgcgga acaattccgg aatcgcaata
     7561 cgataataag ctgtttctgg aatacgatcg ctgattacca catttgtaaa gaattcttta
     7621 ttgattttca agaattccac atctgagttc attcgtgcgt ttttcactga gaagcgtaaa
     7681 agctgtttac tttcaaaatc gatatcatcg tcaatcacat agaaccgaac aaatttagac
     7741 gggttgcaat tatctaatac tgagacaaat aatgcagcta aatgtggtac gaattttgtg
     7801 ttacaacttg cgactactgc tatttctttt ttattcattg atctcgccta ctttcgttag
     7861 agtactgtgt gctaattttt ttaagtataa attggtatac ggatgatctt tcaacgtatt
     7921 ccaaggttta tcatgtcctg taaagtgaac gatcgaaggt ttttcatttc cttctttata
     7981 ctgacgttca tatttttcat ttggtgctgg atgacgttca aaaatcagcg aagtctgcat
     8041 gttccatttt ggatgaagtt gttcccaatc ttcataaagt acagcattca aagcatcttg
     8101 gtcgtgatag atgattcgat cgccattttc actgaggtaa tggatagttt tttctgtaat
     8161 ttctttttta ttccattgat caatgtcaat caccatcact cctgaattga agtacaagtc
     8221 atcggaatcg attcctaatc tttccaaagc tttcacttgg cctggatcga taatagcgcc
     8281 gattgttttt ccatttaacg gctcatcata tagttttacg atatcgtcta gtactaacac
     8341 atctgaatct aagtaaagga ctttcttata atcctcttta gctaataagt ttggtaaaga
     8401 aattcttaag taagcggtag ttgtgatatg gtcactgact aggaagtctt cgtagatatc
     8461 tttttccact gtcaggaatt ggatacttgc attacttgaa tatttattta ccgtttcttc
     8521 caatccttct ttactatatt ctgatagacc atcgtcaatc acataaaatt ttatgcgtct
     8581 tgttttattg caattttcta atgcagttgc gatcatcacg ctcaagtaag gagcataatt
     8641 ttcgtctgaa gcagttacca caggaactac gccgtatctc ttctccatca aaatcactcc
     8701 tttttctaat ctttttctaa cctgttctac atctagaata ttcctcattt ggttttaaag
     8761 caaaatatat gctttgtttt cattgaatat tttaaaattt ttttatcaca aaacgcatcg
     8821 gtaagcgcta actattcgtt tttcatggct ttttattcaa aggtat
//
LOCUS       DAYYKN010000110         8850 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000110 DAYYKN010000000
VERSION     DAYYKN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.111"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1458
                     /locus_tag="ACHBB7_002235"
     CDS             <1..1458
                     /locus_tag="ACHBB7_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM9210153.1"
                     /translation="QQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVAN
                     GGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLAL
                     PFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLA
                     PLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGF
                     IRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEE
                     KPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFI
                     FILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLL
                     GSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPG
                     TESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1637..5164
                     /locus_tag="ACHBB7_002236"
     CDS             1637..5164
                     /locus_tag="ACHBB7_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM9210154.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            5161..>8850
                     /gene="addA"
                     /locus_tag="ACHBB7_002237"
     CDS             5161..>8850
                     /gene="addA"
                     /locus_tag="ACHBB7_002237"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM9210155.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILL"
BASE COUNT         2934 a         1557 c         1859 g         2500 t
ORIGIN      
        1 cagcaagaca agggcttctt cgggcagccg aagggattgt caactttgtt cttcactgag
       61 atgtgggaac gatttagtta ttacggtatg cgtgcattac ttatctatta tatgtacgat
      121 acagttgcca acgggggact tggattacca cgagcaacag cattagctat catgtcgatc
      181 tacggatctt tgatctacat gtctagtatc gtcggcggat gggtatctga ccgagtgtta
      241 ggagcaaaac gcactgtatt cttcggtgga gttttcatta tgcttggcca catcgtcttg
      301 gctttaccat ttggtgtaag tacattgttt gtttcgatgg cattgattat tatcggaacc
      361 gcctttttga aaccaaatgt ttctggcatg gtcggacatc tttattcaaa gactgacttg
      421 cgaagagatg caggattttc gattttttac atgggtgtca acttaggggc attattagca
      481 ccactaatcg ttggtacaat cggtcaagaa tataactatc atttagggtt ctctttagct
      541 gctattggga tgttctttgg attattacaa tacatcatcc aaggacgcaa gacattagac
      601 ggagtgggta caacaccgcc gaatcctttg acaactgaag aacgtaaagg attcatccgt
      661 aacttgatca tcgcacttgt tgttatcgct gcagtattcg gcggggcaca actaactggt
      721 catctgacaa ttgacttttt tgtcaataca atcagtgtat tggggattat cttaccgttg
      781 tattacttta tcaaaatgct gacttcaaaa gatgtaactg ctgaagaaaa accaagagta
      841 tgggcttata tcccattatt ccttgcagca atcattttct ggtcattaca agaacaaggt
      901 tcatctatct tagctttatt tgctagtgaa cgtacacaat ctacgctatt tggtgtaacg
      961 atccaagcaa gctggtatca atcattgaat ccaattttca tctttattct gacaccagta
     1021 ttcgtaacga tgtggacgaa acttggtgaa cgtcaaccat ctacagtagt gaaattctct
     1081 cttggacttt tatttgcagg attatcgtac ttgctattga tgttgccggg tatgctttat
     1141 ggtacaagtg gaaaagtaag tccattgtgg ttgttgggaa gtttcttcat cgtggttatt
     1201 gctgaattgt gtctttcacc agttggtttg tctgtgacaa caaaattagc accacgagca
     1261 tttgaatcac aaacaatcgc tatttggttc ttagctgatg cttcttcaca agcaatcaat
     1321 gcacaaattg cacgcttcta cacaccggga acagaatctg cttacttcgg tattatcggt
     1381 gcagtagcaa tcgtcggtgg tatcttgcta tttactgtga aagaaccaat caaaaaacta
     1441 atgggctcta ttcattaatc aatgaacata aaaacaggaa ttgcttattt gcagcagttc
     1501 ctgttttttg cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg
     1561 aagaatatag aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg
     1621 ttgaaggagg aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg
     1681 tcaagtcttg atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt
     1741 tttccttgtt cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg
     1801 aagaagaagc ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg
     1861 tctggcttgg tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc
     1921 aggatctgca atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt
     1981 tcgcggagaa atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt
     2041 gcgtacagga aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc
     2101 agaagaccag caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga
     2161 attgactcaa cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca
     2221 gacacaagat ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca
     2281 agagtatcaa ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct
     2341 ggatcgtcct tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa
     2401 gctttatttc cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg
     2461 atttgcacca gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga
     2521 gacatctagc caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt
     2581 gtgggaagct gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact
     2641 agtcagtgaa gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt
     2701 atatggaaat atccttcggc cgatctttca agaaatggca attccttttt atatcgatga
     2761 agaacaaatg atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga
     2821 ccgctattct tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc
     2881 gacggaagca gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa
     2941 actggacatc actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg
     3001 tgaaaaagac tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca
     3061 gtatttggaa gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt
     3121 tttgaaaaaa ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt
     3181 tttgatccaa agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag
     3241 aggaaactta gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt
     3301 ggatgaatat gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat
     3361 cattacaagt ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt
     3421 gcgaatcaat cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg
     3481 gttgaatgat caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga
     3541 acgagaagta ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga
     3601 aagtatctct tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt
     3661 atatcttagt tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt
     3721 gcgtagaatc acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga
     3781 aagcgaccct aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat
     3841 ttatcgatta gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga
     3901 attagtactg aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca
     3961 tttaaatgtt cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat
     4021 ttatacttca atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca
     4081 atttggtttg aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga
     4141 tttttatcat gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt
     4201 agtgaatatg tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt
     4261 cggagaactt cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca
     4321 gttaggacag acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg
     4381 catgcatcct cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac
     4441 aggtctggaa ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg
     4501 aatcgatgta gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag
     4561 cggacgttca ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta
     4621 tctggacgta gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc
     4681 aggatcatat tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa
     4741 gaatacattg aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga
     4801 agtactagat cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa
     4861 tgcgaaagat gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga
     4921 actgcaacta ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac
     4981 ttccggagaa atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt
     5041 ttgtccattc agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg
     5101 tctggagaaa atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga
     5161 gtgaatcaaa ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg
     5221 atttttgatc aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca
     5281 gtgcttgttc gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg
     5341 attgtgacat ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta
     5401 caagaatccg tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta
     5461 gttttattgc ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga
     5521 cgttattatt acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa
     5581 acgatcttga tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat
     5641 gatgaacgat ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg
     5701 acgaatctgg tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg
     5761 ctagaacagt taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat
     5821 caaaaacaaa tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa
     5881 cagatgactc agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga
     5941 gaacaacagc agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct
     6001 tatgctggtt tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg
     6061 aaagaaattt ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag
     6121 ataagtaaaa gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca
     6181 ctaccgttag tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg
     6241 cgtaaacggg aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt
     6301 ctcacagaaa aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa
     6361 aaattcaaag aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt
     6421 ttatactggc taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc
     6481 aaacaatcca tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag
     6541 aatttctcaa agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga
     6601 aaagaagtcc tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt
     6661 caaatcaatt atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat
     6721 gaacagtttg agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag
     6781 attccgacgg atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta
     6841 aaaattcgtc aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt
     6901 cgtccgatag agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg
     6961 attttagaga ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat
     7021 tttcaagcga cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat
     7081 caagatattc cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa
     7141 ttagccagca ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat
     7201 caagcatcaa acgaagatga attagcagct aagctggaac attttggcaa gcaactagag
     7261 cattggcgag aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat
     7321 gagacgggat atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat
     7381 ctttatgcat tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac
     7441 cagttcgtgc gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc
     7501 atttcaatag aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa
     7561 ttcccagttg tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat
     7621 cggtatgctt ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga
     7681 gtgttatatg atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg
     7741 tcggaagaaa tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt
     7801 gtcggttcct ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat
     7861 gaggagttag tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg
     7921 attggttacg gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg
     7981 acttctcttc ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg
     8041 atcattgaac aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg
     8101 aaagaagatg aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct
     8161 tatcaagctt cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc
     8221 gaagatccag atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca
     8281 gcatcccgtc aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa
     8341 gatcggcaag tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg
     8401 ccattagaga tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa
     8461 cggttagtag acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt
     8521 caaacagaac ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct
     8581 ttttccatgc tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa
     8641 ctattaatcc acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat
     8701 gattttaaaa cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc
     8761 caaaaatatc aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag
     8821 ccagtagaaa atgttttcct aattttgctt
//
LOCUS       DAYYKN010000111         8796 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000111 DAYYKN010000000
VERSION     DAYYKN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.112"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(394..2775)
                     /locus_tag="ACHBB7_002238"
     CDS             complement(394..2775)
                     /locus_tag="ACHBB7_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM9210156.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2765..3412)
                     /gene="recU"
                     /locus_tag="ACHBB7_002239"
     CDS             complement(2765..3412)
                     /gene="recU"
                     /locus_tag="ACHBB7_002239"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM9210157.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3476..4015
                     /locus_tag="ACHBB7_002240"
     CDS             3476..4015
                     /locus_tag="ACHBB7_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM9210158.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4105..4524
                     /gene="gpsB"
                     /locus_tag="ACHBB7_002241"
     CDS             4105..4524
                     /gene="gpsB"
                     /locus_tag="ACHBB7_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM9210159.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4546..4913
                     /gene="rnpB"
                     /locus_tag="ACHBB7_002242"
     ncRNA           4546..4913
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHBB7_002242"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5122..6288
                     /locus_tag="ACHBB7_002243"
     CDS             5122..6288
                     /locus_tag="ACHBB7_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM9210160.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6334..7830
                     /locus_tag="ACHBB7_002244"
     CDS             6334..7830
                     /locus_tag="ACHBB7_002244"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM9210161.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7911..8594
                     /locus_tag="ACHBB7_002245"
     CDS             7911..8594
                     /locus_tag="ACHBB7_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9210162.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8603..>8796
                     /locus_tag="ACHBB7_002246"
     CDS             8603..>8796
                     /locus_tag="ACHBB7_002246"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210163.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2782 a         1587 c         1856 g         2571 t
ORIGIN      
        1 tcgataaaaa aagatggtgc agacaaatta tgataattaa gcatactatc agataactca
       61 aaatgaagcc ttctttcaaa aaattattca aaaccatata ttgtattttt tatttgataa
      121 taacaatggg attattaaga agtggcacta aataaaagac tagtgtgtat ttagccgatt
      181 taattataaa caagtaaatg gataaagtat atgtaatgat aaaagaaaaa tggattttct
      241 aatttttaag ctaaatgtta gatcacacat gtatatgctg gatagtctta taaaaaatca
      301 tgtaatacag cttggtacat gatttttatt tgtagctgtt ttttacatag acacaaaaaa
      361 gaaaccacgc ttgtagacgt ggtttctttt ttactatctg tttccagaag ctgaggtgct
      421 gctggatggt gtatggtcat ttgcagcatc cgctccgcta gttgctggct cactggaaga
      481 agcaggagga gcagatgaag acggtgtcga gctttcttcg gatgaagaag aaggaaccgt
      541 tgttgaagac gctggctcgc ttgtttctgc tgtcgtgctt tcttttgagg actcagcagt
      601 tgattcgctt tgactagtag agctagagct ttgagtcgtt gtctctgttg ttgagcttcc
      661 aggagtctgg acatagctgg aagatggaat agttgttgat ggtgtaattg cattcgatct
      721 agctgtatat tgatctttgt agtacagttc accgcccaca cgaattaatc cgcttggcat
      781 ctcccagtct gtatttgtca cgttagctga aacatactgc ataagctcgc gataaacatc
      841 agaggctacg tgtgacgatt ctgaagtgac aggcgtcatt tttttattgt aacctgtcca
      901 cacggaaatc gaataatttg gcgtatagcc cgcaaatagg atatctggat acaccccaga
      961 agaaatacca agttttgcat actcgtcatc tgtgtaattg gatgttccgg tcttaccggc
     1021 ttgatataac ccagcgattt gcgcattcgt accagttcct tcagtgatcg tatcttttaa
     1081 aatgtctgtg atcatataag ctgtttctgg agacatggct gtttttccat ctggctcata
     1141 agtttcttct gttccgtctt ggaagacgat tttattaaca tattgaggtt tgtaataagt
     1201 ccctccattg gcgaaagccg cataggctgc tgccattttt aaggaagaag ctccgtattt
     1261 tgtcccatct tgttcttccg tattactaga gatcgcattc gattgatgga tcgtactgta
     1321 ttcgatccca agatttttta agaaagaagc cactttatct gaaccgactt cattgaataa
     1381 tttgactgct ggaacattac gggataagta aagtgcttgg cgtaaagtaa tggtgcccat
     1441 atattgatta tcccagtttc ctacaggagt cgaagtccct tcatagttat acggtgcgtc
     1501 agtaatagtt tttccagtag aatatttcaa atattcaaaa gctggtccgt aatctgtgac
     1561 aggtttcatc gtagaaccaa aatcacggga agtattcact gctaagttgt tgcctaaagt
     1621 tacgtcttct gcgatgtgac gtccgccaat ctgggctttt actttcccgg tattggtgtc
     1681 gataagggtg gaggcaactt gcatctcgtc gtctgggtaa gagacatact gatccgtgtt
     1741 gacgatatca tatagtttct tttgggcgtc cagatccaaa ttcgtataaa tctctaaacc
     1801 gtctgtgtag acattcttat ctgttttttc ttgtacttca ttgatgactt ctttgacata
     1861 attatcgacg atcttagtat tgtcatcgga ttgagttagt tcttgcagac catccgttac
     1921 cggtacatta acagcttgat catattctgt ttgagagatt ttttcgtttt gaagcatcgt
     1981 atacaaaaca gtatctcgtc ttttcttcgc ctgatctgga taaacataag gatcataggc
     2041 ggaaggggct tgaggcatcc cagcaagcaa tgctgtttgc ggtaaagaaa gttctgataa
     2101 tttcttacca aagtacatct cagaagctgt ctccatcccg tacaagccgt ttgacatata
     2161 cactttattt acgtagtagg tcaagatctc ttgtttcgat tttttttgtt ccaatcttac
     2221 agccatccac gcttcttggg ccttgcgttt cagagtctga tcctcggcac tggtggagaa
     2281 gaaggaaagt ttgatcaatt gctgtgtcaa agtacttccg ccttgcagtc cgccagaggt
     2341 aaagttagag agggcagaac cgatgatccg aatagggtca accccgatgt gtttataaaa
     2401 acggcgatct tctaccgaca cgatcgcatc ttctaatgtt ttcggtaatt cattcgcaga
     2461 aattttttca cgcttttctg cgcctaaatc ttcgaatagt tcgccatctt gtgtatatag
     2521 tttggaagat acggtagcgt ctaatttttt atccgtcaat tcgggcgcat cttttgcgta
     2581 ataccagaaa agtcctacac ctgctaaaaa gaggatacag aaaaatgaga gtattcctaa
     2641 aaggatcttg ataaataatc cttttttatg tgttccagag gactttccag aacctttgcc
     2701 agaattcttc ttcacactct ttttaggagt aggctgtttc tgtcgtctag agcttctagt
     2761 ttgttcattt gccattcttg ctttctcctt caatagataa tgttgataaa tagaagtcaa
     2821 ccgcgtccaa ataaggaatc cgcggagaaa taccagtttt gatctctatt cctagttctt
     2881 cgatcaaaga taaaggtagt gatttgcgtc ctttttcctt ttgggcgtcc cagtaagcaa
     2941 tcaaatgaac actattcaaa taaaaacagc gcttcaaaga cgaaaaccat aacaagacaa
     3001 agcagacccc attttgttcg aggcatgctt tcatatgttc gatttgatgg gcatggaagt
     3061 ttttaaaggg aaaagatgtt ttgttcttgg tttcttttgc ttcaaagtcc aagtaaaagc
     3121 ctcgatagac gccattatag tctgtagttg aggcttgggt aaagtaggct tccttgatca
     3181 cagcagcact tctcttagga tagtctactt tcacgatttg gataggggta ggctttttat
     3241 ggataaccgc caacccgcgg gaaagataat atttattact ttcattgatt gcttcttcaa
     3301 agcgcatccc ccgattgccg aattcagtga tctgatttct tttttcttga attttttcga
     3361 cgggagaggc attcttataa taaggttggc catttggata gcgcaatacc atctttaatc
     3421 acactcctag gccacattat agcaaaaaag cggatagaaa gaaaaggaat ctgagatgga
     3481 taagtcaaaa gtaatctatg tgtctgggta tcgaagtttc gaactcggtg tttttaaaga
     3541 aaatgatcct aaaatccaag ttgttaaaaa tgttttaaaa aaagaactcc atcagttagc
     3601 agaagaaggg ctggaatggg tacttgtcag tgggaatttg ggcgtcgaac tatgggctgt
     3661 gcaaatagta gcggaattga aagaagaata tccggaacta tctcttggta ttttgtatcc
     3721 tttttctgat tttggtaata actggaatga agggaaccaa gaaaaaaaac aattagcaga
     3781 acaattagca gattatgtcg aagctgttag ccatcaaccc taccaatcac ctagtcagct
     3841 aaagaatcac actcgtttta tacttgagca tacagtgggc tgtctgctga tctatgatga
     3901 agaattccct ggaaaaacga agtatttttt tgaagatgca cagaattacc aagcagatca
     3961 tccctacgaa attcggctga tcagtatgga cgatcttcaa aattttagtg aataatcgat
     4021 attgtgtttg atttatcaac ttttttccta tacaatgaaa aaggtagaaa tgttattacg
     4081 taaatgaaaa ctagaggtgt aaatatggcg aatttggttt atagtcctaa agacatttta
     4141 caacaagaat tcaaaacaaa aatgcgcggc tatgatcccg ttgaagttga tgaattctta
     4201 gacaatatca tcaaagatta tgaaacttac agcaaagaac tccttgcttt acaagaagaa
     4261 aacgatcgat tgagtgcaaa agtagctcaa ttatctaaaa ctcaaggagc agctcaaaca
     4321 cgcgtgcaac aaacagaggc accaaagagc gcagcagtga ccaattttga tatcttaaaa
     4381 cgcttgtcaa atcttgaacg tgaagtattt ggtaaaaagc ttgatcaaca agcaagtgca
     4441 gtcaaaccag cacaacctaa tccaaataac tacacaaatg cagacacaag cttagatgat
     4501 aatgaaaaaa cacgtcaatt ttaatttttg attaatgaat ttgcggtttt cgggtaatcg
     4561 cggtctgact tgatcaggct gaggaaagtc catgctcgca caggctgtga tgcctgtagt
     4621 gttcgtgctt agcgaaacca taagctaagg tactctttgg agtaacggca ggaaaacaag
     4681 ctaaggcttc ggctatgctt aagtatcctt gaaagtgcca cagtgacgaa gcggctgggg
     4741 aaacccatcc ggtggaacgc ggtaaacccc tcgagcgagc aacccaaaca atggtagggg
     4801 cgctttttgt ctggaagaag aacaggacaa aaaggcagta taactgcaga tagatggtta
     4861 ccgctttgtt ttttctccct ggaaaagcaa agtacagaac atggcttata gaaaaccgca
     4921 aacatcaggg aattaggtcg tgacaaaagt aatgcgttat aagaaacaag aaaaacattt
     4981 gaaagctatt tttcaatact tcagatgaat cgttcttatt acaaaacaaa cttttgtatc
     5041 accggttatt cggaatttga gtaaaagtgt ggggtaggac tgatgtccca cctccttttt
     5101 ttataaataa ggagattgtg tatggaaaaa aacaggaagt tcaacctcgt cgcaactgcg
     5161 gcaagcgggc tagaagcact ggttggtaaa gaattaagag atcttggaat cgaatgccaa
     5221 gttgagaacg gacgtgcacg ttttgaagga acaatggaaa cgatcgctac agctaattta
     5281 tggttacgta cagcagatcg agtaaaaatc gtggttgggg aattcgatgc gtatacgttt
     5341 gatgaattgt ttgaaaacgt aaaagcattg ccttgggaag attttcttcc actagatgca
     5401 gcttttcctg tagctggaaa atcgatcaag tcgaaactat atagcacacc tgattgccaa
     5461 gcgatcacaa aaaaagcaat cgttgaacgt ctccgtacct attatcaccg tccagcttct
     5521 gtaccactga cggaaactgg cgcacatttt cagttggaag tagctttgct gaaagaccat
     5581 gtgatggtaa ctctggatac gacaggacca agtttattca aacgtggtta tcgcttagaa
     5641 aaaggtggag caccattgaa agaaaacatg gcagcagcac ttattatgtt gacaaattgg
     5701 cgaaaagacc gtccatttta tgatcctgta tgtggttcag gtacgctttg tattgaagca
     5761 gccttaatag gccataatat cgcaccagga ttcaatcgtg agtttgcttg cgaatcttgg
     5821 gattggttca gtcaagaaat catggaaaat gttcgatcag cagccgaaga aaaagcagat
     5881 tatgatattg aattagatat tactggttca gatatcaatg gacggatgat tgaaattgcc
     5941 aaagccaatg cagaagaaat cggattaggc tcttcgatta cctttaagca acaagcagtc
     6001 aaagacttca aaacggataa agaatacggg gtcatcgtgg ctaatccgcc ttacggagaa
     6061 cgtctaggag aagaagaaac tgttcggaaa ttatataaag aaatgggcga tgtattccgt
     6121 ccattaaaaa catggagtaa atatatcttg actagcgact tagcgtttga ggaattttac
     6181 ggacaaaaag caaccaaaaa aagaaaattg tacaatggtg cattgcgtac ggatctattc
     6241 cagtattggg gaactcgtcc gcctagaaaa aaagagaaag acgaataatc ttactaattg
     6301 attcattcaa ttggatgttt ggaggaatag ggaatgaaag aaaaagagtt tttacaagaa
     6361 gtaaaagaaa tcaatcttct acaacaagct ttaggaatcc tcgattggga tacacaaaca
     6421 ggtatgcctg aaaaagctag tgcataccga agtgaagtag acagctatct ttacagcatt
     6481 tatttttcaa aaaaaatcgg tccaaaaatc aaagaagcca ttcgctattt ttctgaacat
     6541 ccggaagaat tgtcggaagt tggaactgcc gtttttgcca aagtaaaaga agagtatgat
     6601 ttggaacatc gcgtaccgga agatcttatg agaaaatata cagctgccgt ttcttcagca
     6661 cacggacaat ggcaaaaagc tagagaaaca caacaatttt ctgattttca acacgcattg
     6721 aatcaaaata ttgaattaac aaaacagttg ataccttact ggagaaaaga tgaaaagacg
     6781 aattatgatg ttttgctgaa tcaatacgaa cccgggatga ctgtcgaaat ccttgataag
     6841 gtatttgatc agcttaaaga aggaattctt tcgatccgcc gtacgctcca agaaaaaggg
     6901 actgaacctg aaacagactt tttaaatcgg agaatgacga aaaaacaaca gaagagattt
     6961 gtagtgaaag taatcgaaca acttggttat gattttagtc gtggtcgttt agatgacact
     7021 gtccacccat ttatgactgg aattaatccg aatgatgcac gaatcacgac acgatggaac
     7081 gaaacagatt tcaaaatggc tgtttttgga gtgatccacg aggctggtca tggtatgtat
     7141 gaacaagaca tcgatgataa atatgcgtat acaccggttt atgaaggtac atcaatgggt
     7201 atccatgaat cacaatcgtt gtttaacgag atcattattg gaagtagtcg cagcttctgg
     7261 aaaaagcaat atcctttctt tcaagaatgt gcagaaggaa cattcgatga tattcctttt
     7321 gacaaatttt atcgttcatt gaaccataca aaatcaagtt tggttcgtat cgaggcagac
     7381 agtttgactt accctctcca tatcatcatt cgttatgaaa tcgaaaaaat gattttcaat
     7441 gaaggggtag atgttgctga actacctgaa atatggaatc aaaaatatga agagtatctg
     7501 ggaatacgac caacaaatga tgtggaaggc atcctacaag acgttcattg gtcaggtgca
     7561 agctttggtt attttccctc ttatgcatta gggtacatgt atgcggcaca attacttcat
     7621 tctttgcagc aagaaataga tgtggaaaat gttctagcgt cagacgatta ttctccaatc
     7681 aaaaactgga tgtcagagca tatccatcaa tatggtgcat ctagaaaacc aaatcaatta
     7741 atcatggatg caacacatga accgcttaat ccgcaatacc tgatcgacta tatgaaagac
     7801 ctgtatttta atgtatatca ggtaaaataa aatacaattt acaaagatag ctgaaattca
     7861 tctaatctat agatgttttc agctatttac ttaagaaaga agtggaacga atggataaat
     7921 tacaagtgat cgctgattat agtatggacc aattagcgca tgatcaaaca ggacatggga
     7981 gcgatcatac caaaagagtt gtcaagttag ctgaaaaaat tttggagacg gaaccgcagg
     8041 cagaccgctt tgtgacgctt gcagcagctt acctacatga tacgattgat gataaagtcg
     8101 ttaaaaatga gaacgaggca aagcaacagc ttcgtgtatt ccttcgctct ttgccaatag
     8161 tagaggaaca gatctcgatg attttttcca ttatcgaaaa tatgtccttt tctaaaaatc
     8221 tctctgaagc ggccgaacta tctctggaag gtaagatcgt tcaagacgct gatcgtatcg
     8281 aagcattagg agcaatcggc attttgcgta ctgcttattt cggcggtgga catgggcacc
     8341 cgatttttga tcctgaactg tatccgcaaa cattcaagga caaagaaaat taccgaaaag
     8401 gcacgacagt gatcaatcat ttttatgaaa aactgttctt gctgtctgat aaaatgaata
     8461 ctgattacgg atatgaagaa gccaaacgaa gagaaacctt catgcgtgaa ttcttagaag
     8521 aattttttct ggaatggtct attgacgatg aaaattttac tccagacaat tggatcaaaa
     8581 ataacaaaaa atgatggagg ttgtgacaga agtggtcagc tccaagatat ttcggatcat
     8641 tttccgtaaa agttacttct ggaacactgt ttaccaagag gtcgtgagcc tgccgttcag
     8701 ctccgagtca caaggaacaa tttcaaaaaa tagcttctcg tgtttgatga atattgtaac
     8761 ttgtgattcg gaattggcag gagaacaccg tttatt
//
LOCUS       DAYYKN010000112         8731 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000112 DAYYKN010000000
VERSION     DAYYKN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.113"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACHBB7_002247"
     tRNA            2..74
                     /locus_tag="ACHBB7_002247"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACHBB7_002248"
     tRNA            78..150
                     /locus_tag="ACHBB7_002248"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACHBB7_002249"
     tRNA            155..225
                     /locus_tag="ACHBB7_002249"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACHBB7_002250"
     tRNA            238..311
                     /locus_tag="ACHBB7_002250"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACHBB7_002251"
     tRNA            323..394
                     /locus_tag="ACHBB7_002251"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACHBB7_002252"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACHBB7_002252"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM9210164.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACHBB7_002253"
     CDS             1983..3455
                     /locus_tag="ACHBB7_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM9210165.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACHBB7_002254"
     CDS             3538..4326
                     /locus_tag="ACHBB7_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9210166.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACHBB7_002255"
     CDS             complement(4393..5061)
                     /locus_tag="ACHBB7_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM9210167.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACHBB7_002256"
     CDS             5176..6888
                     /locus_tag="ACHBB7_002256"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9210168.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACHBB7_002257"
     CDS             6891..8663
                     /locus_tag="ACHBB7_002257"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9210169.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2622 a         1487 c         1944 g         2678 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc a
//
LOCUS       DAYYKN010000113         8675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000113 DAYYKN010000000
VERSION     DAYYKN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.114"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..271
                     /locus_tag="ACHBB7_002258"
     CDS             <1..271
                     /locus_tag="ACHBB7_002258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210170.1"
                     /translation="DQLILGFIYICLDEKLSNRQKSLKSINYEVFNYLGVAFGLIDSE
                     IKSTNAGNDEDLLNYLKELTSFIDEIKTTTFNKKKYFQNQYDIEE"
     gene            325..1470
                     /locus_tag="ACHBB7_002259"
     CDS             325..1470
                     /locus_tag="ACHBB7_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9210171.1"
                     /translation="MATIKQYEKKDGTKAWMFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKRETNETFQEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLETLFQYLESKRSTYRAEYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(1555..1860)
                     /locus_tag="ACHBB7_002260"
     CDS             complement(1555..1860)
                     /locus_tag="ACHBB7_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM9210172.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(1885..2316)
                     /gene="ruvX"
                     /locus_tag="ACHBB7_002261"
     CDS             complement(1885..2316)
                     /gene="ruvX"
                     /locus_tag="ACHBB7_002261"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM9210173.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(2313..2579)
                     /locus_tag="ACHBB7_002262"
     CDS             complement(2313..2579)
                     /locus_tag="ACHBB7_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM9210174.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(2834..3559)
                     /locus_tag="ACHBB7_002263"
     CDS             complement(2834..3559)
                     /locus_tag="ACHBB7_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM9210175.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(3920..3990)
                     /locus_tag="ACHBB7_002264"
     tRNA            complement(3920..3990)
                     /locus_tag="ACHBB7_002264"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3956..3958),aa:Gly,seq:ccc)
     gene            4107..4859
                     /locus_tag="ACHBB7_002265"
     CDS             4107..4859
                     /locus_tag="ACHBB7_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9210176.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNNLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4875..5354
                     /locus_tag="ACHBB7_002266"
     CDS             4875..5354
                     /locus_tag="ACHBB7_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9210177.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(5605..7185)
                     /locus_tag="ACHBB7_002267"
     CDS             complement(5605..7185)
                     /locus_tag="ACHBB7_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM9210178.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(7178..7921)
                     /locus_tag="ACHBB7_002268"
     CDS             complement(7178..7921)
                     /locus_tag="ACHBB7_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9210179.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(8097..>8675)
                     /locus_tag="ACHBB7_002269"
     CDS             complement(8097..>8675)
                     /locus_tag="ACHBB7_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9210180.1"
                     /translation="NAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVINRKITPSG
                     YLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHYDEILYLMS
                     MPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITSISRVNEI
                     VTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         2753 a         1662 c         1454 g         2806 t
ORIGIN      
        1 tgaccaactc atcctgggtt tcatatatat ttgtcttgat gaaaaacttt caaacagaca
       61 gaaaagtctt aaaagtatca attatgaggt attcaattat ttaggtgttg catttggttt
      121 aattgatagc gaaatcaaat ctacaaacgc aggtaacgat gaagatttat taaattattt
      181 aaaagaatta acatctttta ttgatgaaat aaaaacaact acattcaaca aaaaaaaata
      241 ttttcaaaat caatatgata tagaagaata aagattacat tttcctatca aaagcacgcc
      301 acgcaactga taggaggaaa aacaatggca acaattaaac aatatgaaaa gaaagacgga
      361 accaaagctt ggatgtttca aacatacctt ggaatcagcc cgctaactgg taaagaagtc
      421 cgtactacac gtagaaattt taaaacaaaa aaagaagctc aaatagagct taatagacta
      481 cttgtagact ttgaagagaa tggacttaaa agggaaacga acgaaacttt ccaagaagtg
      541 tatgatctct ggtatgaaag ctataaaact actgtaaaag aagtcactca attaaaaatt
      601 gagatcaagt ttaataaatg gattcttcca aaatatgggc agtttagaat taaagaaatt
      661 actacaaaac atgttcagaa gatattgaat cagtgggcta aaacaacaga tcaatataaa
      721 gtcctacatt ctaccgtaag ccgtattttc aaatacgcaa tgactctagg gctgatcact
      781 aaaaatccct gtgagctcgt aatcatgccc aacagaaatg ttgaaaaaaa gaaggatact
      841 gtcaaaattt attcacgaga tcaattagaa accttatttc aatatttgga atccaaaaga
      901 agtacgtata gggcagagta cgataaaact ttactgcgct ttctattttt tagtggctgt
      961 cgaatcagtg aggctttagc cttagactgg tcagatattg actttgattc aaacaccgtc
     1021 actataaaca aaaccttaag ccaaacaaaa tatgggtaca aaatttcaag tccaaaaacg
     1081 agaaatagct atggttccct atctttagac aataaaacca ttaattggct aaagaaatgg
     1141 caaatcaatc aaaaaagata tatgttaacc ctcgggatta ctgatccaac aatgattttt
     1201 tgtggaatct acaaagaaat cattactaaa cacgctatct attctcgtat gatcacaatc
     1261 tgcaagaatt ctggtattcc atttctaggt aaccatgtaa ccagacacac tcatgctagt
     1321 atgctgttag agtcgggagt ctccatgaag gaagttcaag aacgccttag acactcatca
     1381 atcaaattaa caatggatac ctatgctcat ctaactaaag aaaccaaaga aaaaaccgtt
     1441 gaaaagcttg cagagcacct aaatttctag cggaacgtat tcaatcccgt attcaattca
     1501 aaacaaaaaa aagctgaacc cttatctacc aagggttcag ctatcattta cagcttattc
     1561 ttcttcttcc gccatgaatg tattgaatac ttcttcaatc atgtcccatt ctgcgtctgt
     1621 ttcgatttgt tctaactcgc cttctgttcc atcttcgttt tcgatgtatg cataagcttg
     1681 taattctaca tcttcatctt ctgaaacacc agcaggatat aaaagaacat agttacgtcc
     1741 aaattcttct tgtccgtcaa ctgttagtag gatttcatat aatgtttcat ttccttggtc
     1801 atccaccaat gtgatgtgtt cgtgtccttc atggtcgtgg gaatggttat gttctgtcat
     1861 aagattacct cttttctttt attattaaaa taattgctta cctgagccgt ctaaataatt
     1921 ttgtaagatc atcacagctg ctaatttatc gatgactttt tttcgtttgg ctcttgaagt
     1981 atttgcctgc tctactagca tacgttctgc ctgaacagtg gttaatcgtt catcttgata
     2041 atcaactggc aaggagaata gttctttgat cttttctccg tatgacatgg aagcttctgc
     2101 ccttggtccg atggaattgt tcatattttt aggtaatcca actacaaacc ggtctacttc
     2161 atattgtgct actaattcct tcaaccgatc aaagccaaac tcttcttctg cttcattgat
     2221 ccgaatgatc tctactcctt gagctgtcca gccaaagggg tcactgatag caacacctac
     2281 tgtcttagaa ccgacatcca agcccattac tctcataaat tcactccatg atttgagagg
     2341 tagtccttga caattacttc catgatctca tcgcgttcgt gacggcgaat caaatttcga
     2401 gcatcacgat aacgaggaat gtaggccggg tctccagaaa gcaagtagcc gacaatctga
     2461 ttgattggat tgtagccttt ttcttccaaa gcccgataaa caatcgctaa tgtttcgctc
     2521 acttcttttt tgcgactatc gtcaaaacca aaacgtacgg tttcatctgt aaaacccatg
     2581 attctacacc tctttcccgc aaggtaattc cagcttacct tctacaacct tattttactc
     2641 gaagaaaggg aaaactacaa acaaatattc caaacttccg aataaattaa gaaacactat
     2701 ttgtatgttt ttttcctttt ttctgtagct ttatcaaaag tactcttttc ataaagtata
     2761 cgctatattt ctttgaatgt atgtttttac acaatttttt cataatattc ataaaaaatt
     2821 cgaatagctg ctactaattt tttgtttttg ttgcttgtcc tgtctcataa ttgatcgtgt
     2881 agccatcttg aacattgtag ataaagacat tgaattcaat ttttttgtct tcaatgcttt
     2941 ctgcttccaa ttgcactccc ctggctacca gttcattccc ctcaaaatga ggagtgacac
     3001 ggtagcgtac atgatgattt gtttctttga tatatgccgc aatatcattt tcgtgagcaa
     3061 gcatgtatgg agcattcaac gaacgtgtcc ctgtcatcaa gtttttaatg ttgttatttt
     3121 gaccagtcag ctggaaacca attaaatggc tgcgattata taaccattgc ccgtcactta
     3181 atttcttttg tttccaacct gtcgggtcaa tatacaaagg ctctctcttt tcagtaggga
     3241 acaaatcttt tccaagcata gcattggctt gtcctactct gttcaattta tcaatatttg
     3301 aatagctttc ccatgttcct ttggaaaggc taagatcctc ttttgtaaat tccggctgat
     3361 tcccattgat ttctatttct tgatgttttc catcatattc tggcacatcc gctacttgag
     3421 aagcttgtgg tgtggaatca aaaatagttg taatctgctg aaagatatct gaatcttttt
     3481 gtgtggtgga aacaccgaat gcccctgcaa tcacagcagc gacgatcccc acgatcgcct
     3541 tggttgtttt tttgctcatt tctttactcc tttactaaca gctccctaaa tcttaacata
     3601 tgttccccta taaataaata ctagaaagaa atagaaacag caaaaaataa aacctgtttt
     3661 aagatggctt aaaaatacaa aaaagagacg gattcctcgt ctctaatcag tcaccactct
     3721 atctgtaagg tatttattat tattcaaaaa ttttcttcat actcaagaca atacactgat
     3781 ttcacaaccg cttggtaaac ggtgcgcaaa agcttgcatc tgcggtaata agtccatcca
     3841 agttaaaaac ataagaagtt gtttttgctt ggctgttctt attacttgtt gcagagcagc
     3901 tttttcacaa cctattttaa gcgggtgacg ggaatcgaac ccgcgacatc agcttgggaa
     3961 gctgggattt taccactaaa ctacacccgc aatacaaaaa cagtatacgc ctcttttatt
     4021 ccttcctcaa gtttttttat tctttacgct tctggtatgt tttgtagtaa aataaaacta
     4081 atcaatcaat tattggagaa gatgctatga cgggaaaaga attagatctg ttgctggaca
     4141 cttgtttact cgctgggaaa atcatgatgg aaagcaatgc ggagatgtat cgggtagaag
     4201 atacgatgag tcgaattgct ttagcttctg gaaatttccg tttggtcagc tatgtcactc
     4261 aaaccggtct ctttattggt cttgatcgta cctcgaccat ccgaatggaa caaattacca
     4321 accgttcgat taatttagaa aaagtagtaa aagtaaataa tctttcacgt aaatatgttg
     4381 cttcagagct aacattagaa gaactttact gcgaactgca agcaatcgac cgagaccgtt
     4441 ctttctttcc tgtctggttg caaatcgtta gcgcggcagt gatcagtgga acaattatgg
     4501 tgttatttgg cggaaagatg accgatttac ctgtgacttt agtcattgga ggattgggat
     4561 acgcagctta tctatatagt ttgaaatttt ttagaatcaa atttttatcg gaatttttat
     4621 cttctttatt gattggcata gccgctatct acagcgtacg ttttggctta ggaagcaatc
     4681 aggatctaat cattatcgga tgtgtgatgc ctttagttcc tggagttcaa attacgaacg
     4741 ctatccgtga tttgctagct ggacattatg tctcaggagt ttctcgcgga acagaagcga
     4801 tgatgactgc tacgatgatc ggttttgcga ttgcttttat tttccagctt ttttattagg
     4861 aaaggaacac aagtatgggg acgatattta ttcagttttc ttttagtttt ttagcgactg
     4921 cggcatttgc cgttattaca aatgtaccta ggcgttcatt gatttgttgt ggtctttctg
     4981 gtgcttttgg ctggatgatc tactggatct gtgttcaaat aggcggatct gctgcttttg
     5041 gttcattgct tggttcttta ggagtagcat tcatcagcga tcttttctct aaacgtttga
     5101 aaatgcccgt aacgattttc aatattcccg ggatggttcc tctagtccct ggtggtttag
     5161 cttatcaagc ggttcgaaat ctcgttactg aatcctatcg agaagcggct cattatgcgg
     5221 tccaagcaat tatgattgct ggagcaatcg ctttaggtct tgtattatca gaagtcttta
     5281 accataacat ccgtaatttc aaagtaaagc gtgacgcaat catgatgaaa cgaaaaaaac
     5341 ggctttttaa ataaacgaag tgtaacaaag aaaaggttgt gaaagagacg ttcagccttg
     5401 agcgatgaca aaatatctca caaacattgc gacaagtaac cgcaggagca ataagaagta
     5461 attatgagta ttaaaaaaag ttttgtaaaa atagcttctc acatttttac ataaaattga
     5521 cttaatactg agaaactgac ttttgaacac cctctatctg taaaaaaggg gctgcgacat
     5581 gatttttgtc acggcccctc ttctttattc aaattttttc caaaacgttc gttgataata
     5641 aacgatccat agagcggaaa gtattagaac taaaccgatc acaggaatat tcactaacca
     5701 aaatggataa aacaaagcca acattccgta taagacgata agaataatac tgatgatcaa
     5761 tgaacccatc atagttgcga ctaaacccaa acttcctgaa cctctgttaa ataattggtt
     5821 aatattggtc cagttcagca gcagcaaccg ataatctcta gcgaaataac gtagtgacaa
     5881 taagtagcag ccgaataaag aaccaaatag caagctaggg ataaaaaaca aaggcagctg
     5941 aaaagacagt ccgcttaata atgcgatccc accagttaaa aaactctgca atatccatcc
     6001 gaatctgaac ttttctttta aatattggtt catagagatt ggcaaagaac ggataaacag
     6061 aaaattttct tgatccaaag aaataagatt gctgataaaa gaagtctgat taactgttaa
     6121 gaaagccaat gcgatcccta ccaggaaaac aacaccgatt aacttattat ctaaatcagt
     6181 taattttatt tcgcctccta gggcaaaagc aagaataaaa atgattggag ttaacaagga
     6241 agaagaaaga acttgcataa tcaaattagg ttcttttact aattgagcat tgtaactaaa
     6301 taaaagctga tgcacatttt ggttcttttt atgggttctt tttattttga actcactgtt
     6361 tgaatcagtc agttgctctg taagctttgg tactatcagc tgttttacag caaggaaaaa
     6421 tcccagagcc gtgatagcta gtattcccca acccaataaa ccttgttgag aaagtggctg
     6481 actggcagca tcatagaatg gcaataatat cgaaatcgga tgccgatctg ttaactcatt
     6541 ttcaatagtc cccgtttgat gattcatcca catgattcca gcaaatgcga tcagcatcga
     6601 gctgccaagc aagatactag aaactgtttt tctatgtttt ttaaacactg ctgttcgagc
     6661 caaaccaaaa acaatcaaac tagccagaca aaaaagaata atcaaaaata gagaaaacaa
     6721 aaggattgag ataatgatag taagcaaaat ccctagatca gctcgccatc cggttaaaat
     6781 aaacatgact agaagcggaa aaacaaaagg aatgatggtc aaagcaacta ccaaaatctt
     6841 tgctgaaaaa acctgttctt gtccaaaagg cagaggcaaa aatacttgca ggtcatttcc
     6901 ttcaaaaaag atattataaa tgactgatat actttgagtt aaacctaaca taccaaaaag
     6961 tgccacgtaa taagtaaaga aacctggcaa tttaacaaag ttcatagcaa acatagcaaa
     7021 tccgtaaatc agtaaaaaaa tcagccctga aaaaagattt tgagacaaaa gatagatagt
     7081 taatcgcttt cccttttttc ctttctctct tgctttattc gtgagctgtg gattaacata
     7141 taaaagatta actcgaatta attctaataa ttgtcgctta tacatacggt ccatctcctg
     7201 tcgcttgcct tccagccatt ttcaaataaa tcttttctag cgtttcatca ggtgagccat
     7261 tcaataattc actaactgcc ccctgataaa tcaattcacc tttcttcaaa atagccagtt
     7321 tatcgcataa ctgttgtgcg gtatccaata catgtgtaga aaaaatgact gtttttcctt
     7381 ttgcggcatg agccttcatc atttctttca aatcaaatgc tgcttgagga tcaagtcctt
     7441 gcaaaggttc atccaatacc caaatatctg gatctggcag caacgtgccg atcaaaatcg
     7501 ttttttgacg cattccatgt gaaaaacttg cgattgtttc attttgatga ctatacatat
     7561 caaaaagcgc tgtcaattct gctaaacgct tttctttttt ttgtgtgtct aattcatagg
     7621 cagctgaaat caagtcccaa tattctccag ccgtcaattg taaaaaaata tctggggaat
     7681 caggcacata accgattttt tgtttgatac tcaatcgatt ttccgtcaat ttcattccat
     7741 ctactaaaat cgttcccgat gatggttcaa taatactaac taaactttta atcgtagttg
     7801 attttccggc accattatgt cctaaaaatc caaaaatttc cccttgtctt atcgagagag
     7861 acaagtcttt taatgcttgt tttttcccgt aacttttact tacattgatg aattcaatca
     7921 tttctgtact ccttttgttt gttattactt tttattgtac ccttcgttat tggtggagta
     7981 aatgtcaaaa agagtgcata acaaaaaagg atagaaacga ctaggctgct acttgggttg
     8041 ttctcttcta caggaaaaca ctcttctaac aggtaagata tgagtctttg ttttctttaa
     8101 aatgattcat tttctatctt ttcttgcata aaattcttta gtttatcaat acttctcttt
     8161 cccatctcta gttgttctcc atttgcaaat gttactattt cgtttactct gctaatagat
     8221 gtaatgtttt cagtattaat gatataagct tttaaatctt tacataaata ctcatattcg
     8281 agtgattttt tatatttttt aattgtgccg tttacgagaa tctcttcatc tttcaatact
     8341 aggcctattt tatttttata acctggcatg ctcattagat aaaggatttc atcgtaatgg
     8401 atatagtgat cgctattacc ataacggaca gttgtccacc tttttttcgt agcatgttga
     8461 acaggatagt tttctttttc tttcaataag taactttgga tgcttttttt atttttttca
     8521 taagtgaatt cttttatcaa ataaccacta ggcgtaatct ttcgattaat aacagttatt
     8581 gctttattcg gatgagacgt tagataaaca attttacact tttcattctt ttctctaagc
     8641 atttcgccta gttcgatacc gctataatag gcgtt
//
LOCUS       DAYYKN010000114         8556 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000114 DAYYKN010000000
VERSION     DAYYKN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.115"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..610)
                     /locus_tag="ACHBB7_002270"
     CDS             complement(113..610)
                     /locus_tag="ACHBB7_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM9210181.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(740..1450)
                     /gene="budA"
                     /locus_tag="ACHBB7_002271"
     CDS             complement(740..1450)
                     /gene="budA"
                     /locus_tag="ACHBB7_002271"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM9210182.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1463..3127)
                     /gene="alsS"
                     /locus_tag="ACHBB7_002272"
     CDS             complement(1463..3127)
                     /gene="alsS"
                     /locus_tag="ACHBB7_002272"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM9210183.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3332..3994)
                     /locus_tag="ACHBB7_002273"
     CDS             complement(3332..3994)
                     /locus_tag="ACHBB7_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9210184.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(4004..4819)
                     /locus_tag="ACHBB7_002274"
     CDS             complement(4004..4819)
                     /locus_tag="ACHBB7_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9210185.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5081..5524)
                     /locus_tag="ACHBB7_002275"
     CDS             complement(5081..5524)
                     /locus_tag="ACHBB7_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFM9210186.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5658..5996)
                     /locus_tag="ACHBB7_002276"
     CDS             complement(5658..5996)
                     /locus_tag="ACHBB7_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9210187.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5984..6361)
                     /locus_tag="ACHBB7_002277"
     CDS             complement(5984..6361)
                     /locus_tag="ACHBB7_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9210188.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6585..7778
                     /locus_tag="ACHBB7_002278"
     CDS             6585..7778
                     /locus_tag="ACHBB7_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9210189.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7944..8366)
                     /locus_tag="ACHBB7_002279"
     CDS             complement(7944..8366)
                     /locus_tag="ACHBB7_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210190.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2524 a         1718 c         1428 g         2886 t
ORIGIN      
        1 taacacttgt gattttataa aaaaataaaa aaaccgaaaa acagaaatgc ccactgtctt
       61 tccggctctt tttattcttg atttctcttc ttttttgttt caatagacga ttttagttat
      121 taataaatga aaaagcattt atattaaaat ttgctaataa aagatctaca tatttttcta
      181 gaaatgcttg atcgatttca tcataccctt tcgttactcc gcaatcgata tctaaaacac
      241 cgattaaatt accttctttt accattggca ctacgatttc tgattgtgcg gctgaatcgc
      301 aggaaatgta atttgcatgg gttttgacat catcaacgat caatgtttct tgtttttcag
      361 ctgattctcc acaaactcct tttcccatct tgatacgtgt gcaagaaact cgcccttgga
      421 acggtcctaa aatcaattca ttaccatcga ataaataata tcctgcaaag acagtctcag
      481 gcaaagtctc attcaacaat gcagatgaat ttgctaaatt tgctaaccag ttatcttctg
      541 cttctaataa tccttgttgc tgtaataata aaatttcgta tgcatctttt ttctgttttt
      601 ctgtccacat gataaaaacc gctccttttt gatctatgga gaagattata gctcagtttt
      661 tttgtaaacg ctaggttttc tcctcaaaaa aacgagatac gtcaggttaa tcaccttgaa
      721 cgcatctcgc tgtctttttc tattctgctt gttggatttc tgctgctaaa tcagcataat
      781 caatcgtttc gtgaacaaaa gtcggatcat caactggaaa atgctgtaca agggacgaga
      841 tgcggctaat ctcaactttt ccttcagcca cttcaaaatc cattacatgt ccgccaaatg
      901 tatgtgtatc atcgataaaa tgcaggtgaa atcccgctgc agcaactcct tggaataatt
      961 caggcgtaaa gaacccgact aatgttcctt ggacattgct ttgttggaat tctggttgag
     1021 actctgaaat ttttgctagt cttctgtaag gtttttcttg cttcggcatg atacggatgt
     1081 gcatattttt gaataatcca gttatcttca ctgccaaaaa taggttttcc ccggcttctc
     1141 ttactaatat gtcttttttc aattgttctc catctgcagt ttttgagaca gaaaaactag
     1201 tatctggcgt aaaatctgta attgctgcat agggggtaag ttcacttcca tctaattggg
     1261 caactgagcc atctgatctt ccttgataag ctacgccatc taaaaagatg acttcaccat
     1321 caagcccatg caatgttccc aatcccagag ttcctttttc taatatatga gcaatcgttt
     1381 ctgttccatc catcaagcca gccatcaaag ctcctaatgt cccgtgctgg tataagattt
     1441 tttcagtcat gtcgacactt ccttaataga attgatctgg taagattgtt tctcccaatt
     1501 tctcattatc acggtaatca atcggaatat caataattac gggtccttct gtcgcaaatc
     1561 cttcttctaa agctttttct aattcctcta cacttgtagc tcggattcct ttagcgccaa
     1621 aagcttctgc atatttcaca aaatcaactg gaccaaagtc tacacctgac gcccgctgat
     1681 atttcatttt ttcttggaac tctaccatat tgtagtgtcc atcattccaa atcaaatgaa
     1741 cgatattcaa ttttttacgt accgccgttt ctaaatcctg ggcagagaat aaaaatcccc
     1801 catcgccgga aactgatacg atttgtgtat ttggacgcac caatgcagca gaaatcgccc
     1861 atggcaatgc aacaccaagc gtttgcatac cattactgaa taacaaatga cgaggttcat
     1921 aacttctaaa gtggcgtgcc atccaaatat aatgactacc tacatctacg gtaacagtca
     1981 tatcatctgt tacttttgac tgaagcgtgt taataacttc taaaggatgc aagatacctg
     2041 cttctccttt tgattgaaca atgtcccgct ccgttaattc tgcttgaaga gatgctaaat
     2101 attcttttgc atcttctgac acttgttgag gctctaatga acctgtcaat aaatcaagag
     2161 tggctgagat atcaccgatc aattctcttt caggctgcat aaaaggatcg atttcagctg
     2221 gcgcttcatc gatcacaatg atccgcgcat ctttttccgc attccagttt cttgcttcat
     2281 attcgattgg atcatagcca atagcaataa ctagatcact tcgtttcaaa agcatatctc
     2341 ctggttggtt tctaaataaa cccacacgac caaagaaatg cgcttctaat tcacgtgaga
     2401 tcactccagc tgcttggaat gtctctacaa caggcaatgc tgtctttcca actaattgcc
     2461 tgatggctaa cgtttctttt tcacttgatc ctcgcatacc tactaatagt accggtagtt
     2521 ttgcttctct aatgcgttca gctaggtaac ggatatcatc tgcagaagca gaacctaatt
     2581 taggatcgct caatggcttg atgacattcc cttggacagg tgcatccact acatcttgcg
     2641 ggatactgat aaagctcgcg cctttttttg aactcttagc catacgataa gcattggcaa
     2701 tcacttctga aattgtttct ggatcttgga tttctgcact gtattttgtg attggctgga
     2761 acagtgctgc attatccatg ctttgatggg tcaacttcaa tagatcgctt cgttttactt
     2821 gtccggcaat tgctaaaact gggtcacctt ctgctgtcgc tgttaccaaa cctgtagcta
     2881 agttactagc accaggacca cttgttgcga taactactcc tggctctcct gtaatacgtc
     2941 cgattgcttg tgccataaat gcagcatttt gttcgtgacg agtcacgatt aattcaggac
     3001 cttgatcctc taattcatta aatacaccat cgatttttgc acctggtata ccgaatacat
     3061 aatcaacatg atgattaatc aaactttcta ctacagattt cgaaccttgt ttcatttttt
     3121 cgtccattga acatcgtcct ttttatgctt tatttttaat tattagttag aaataatcac
     3181 aaataattga tacagtttct aaacctgttt cattcaaaag tatagcacgt tttatttcat
     3241 ttgtttagcc atatgtttca aaaaatgaaa aagaatgaca gtttttacat aaaaactgtc
     3301 attcttaatt gaaagtttca atttataaag tctacagatc catagttctc atcaatttac
     3361 gattgttgaa gtaaatcaaa ccaaatacga tagaaggacc tagtagaata caccattggg
     3421 gctttacgaa aaagaagaga aacaaacttg tcaatagtaa aaaatctagt aaactcacat
     3481 accatttttt caacgtatag tagaacgcta tccgtagaac ttgatttggt ttcgacgctg
     3541 gatttctcac ctgaaaatac cagtagttga tgctaaagga aactgataaa caagcaagta
     3601 aaacaaacaa cggaatcaac cattttccat aagaaaaacg tataaaaaag aaacaatcaa
     3661 aaatcattat cacactaact atccaagcaa atagccaata aaaaaatccc tttagccaaa
     3721 attggcgaaa cgtctttatg aacaaacgta ctggtgaaaa acttttttct tttgctaatt
     3781 cttctgtcat acctatcatc acgatgcaac ctggaccaat aaaaagtaaa gaaaaaataa
     3841 aggaaaagac attccttcca tctatcgaca aaaggaacgc agctgctata tacaagatat
     3901 tgacacacca aaaacaaagg ctacttgcca atgattgata caaccaataa aagaattcca
     3961 tataaacatt tttgtcaaaa cgctgctctt tcataatcac ctcctattcc ttcattcctg
     4021 atgaagcgat cccttctaca aaatattttt gcatcgctaa aaatataaaa gcaacaggaa
     4081 gaacagaaac aactgaggca gccatgatta atgcgtactc tgcatcaaat agcccaataa
     4141 acattttcaa ccctaactgg attgttttat tttccgtaga agacaaataa atgaatggcc
     4201 ccatataatc gttccatgta ttaacgaatg tgatgatcgt caaactggca atcgccggtt
     4261 ttgtaagagg taaaatgatc ttgcgataga ttccccactc tgataagcca tcgatccgcg
     4321 cactctcaca taaagaatct gggatactac tgtagtattg cttcaacaag aacacaccaa
     4381 aagcattgaa cgcttgtaat agcaccaacg accacaacgt atatgttaaa cccacttttc
     4441 tcatcataat gaactgcggg atcatataag actgccaagg gacagcaatc gtgccgatat
     4501 atgctaaaaa taaaatatcg cgccctttaa aatttagttt agaaaacccg taagcagcaa
     4561 aactagaagt aaacaactga atcaacgtga tcacaacgct taaaaaagct gtattcttaa
     4621 aaaatgtcac taaaggaata cgagaccaaa taaccagata attctctaaa tgccaagttt
     4681 tagggatcca ttgaatcgga atcgtgaaca cttcattatt tgctttgaat gaagcagaaa
     4741 ccatccataa aaaaggaatg atcgtcaata aagctaatac aatcagcaga atcataccca
     4801 accattgaat aaagggcatg ttttttgaac gaatttttgc tttttttctt tcatttcgaa
     4861 cacctccaaa gttataatct tcatttcttt gtaatgatta caatccagaa caacggttat
     4921 tatagctgat tcatttccgt tcttcttatg tttgtaagcg cttatttaac aagttatgta
     4981 aatctcttta ttttttttga tttaaaaatg aaaaaataca aaagcaagac tgtcatacgt
     5041 ctcgcttttg tattgaaatt tcttcgatta gagatactta ttactgttca aaagcttctt
     5101 tgaaacggtg aatcaattca ttgactacag gtgataaaat ccgattcttt ttccagacta
     5161 gcatgcagtt cgtttcaatc attgggtcaa gcggaacgaa tgtcactcgc tttgaaagat
     5221 gacttacagc accttctatg actaaagcag cacctacctg attctcaatt agcgcaaata
     5281 cattgaaaat caaattgtac gttcctgtga tcagcaactg ctcctttttt atgcctaacc
     5341 agtcagcaaa cattcgctgt atttctggac gctttgagat caaaagaggt acattcgcaa
     5401 agtcttcttt tgttatcacg tctttttttg ctaaaacaga attttctaaa atgagcaatc
     5461 cccatttttc ctttcttggt aacaaaatcg tttcgtattt ctctgtatct actggatcta
     5521 gcaaaatcgc taaatctaac aggctttttt ctagtcactc cgtgatatca tcacttgttc
     5581 cgctgtggat atgaaattga acgctgggat aatcacggat caacttttct agtaatagtg
     5641 ccaatgtatt cgaattatca gcttccacac agccgatcgt aaatcggccg ctgaataatt
     5701 gtttttttct gttctcgaat tcttgagtgg tttgattcgt taaatctaaa atttcttctg
     5761 ctcggctttt ttagaaaata acctgcttct gtcaagatca ttttgcggtt ttcgcgtaca
     5821 aacagttctg ttcctagttc ctcttctaac tccttcagtt gtcggcttaa ggttggttgt
     5881 gtaagatgta gaacttctgc ggcttgagag attgttcctt cttctgcaat tgtccaaaaa
     5941 tattttagta agcgtaactc catagcgatg ctccttgatt tttttacttg atacattcca
     6001 ttttatttct agataacaca cgatcatatt tcttgatttt tccttgtaaa cgttccaaac
     6061 attcttgcat ttcagcaatt tttttcgtca aaagttcact ttcttcaatc aaaagcttac
     6121 gccgctcaaa tacagttgct tctccttcgc tagataggcg aatatattca atcaaagact
     6181 cgatcgataa tccggcattt cgcatacaga tcgtaaagtc gacccatttt aggtcttctt
     6241 ctgtataatc acggattccg ccttttgtcc gattaactgg tggaatcaac ccgattcgtt
     6301 cataatagcg caatgtatcg gccgaaatgc ccttttcttc actgacttgt ttgatattca
     6361 tctgatcact ctttctctta atttcttgcc actttactta tgtattttat ttattctaca
     6421 actttaagtg tactccaagt caatactcat ttctattaaa actaagctgg attatctata
     6481 tcggttgttt gtgtattatt tcaaataaaa aagtcttgtt tttttattct tttttggata
     6541 tacttttaac aatttaggga aattttagga aaaggggtat aaaaatgaca acgtatcagc
     6601 gaaagttatt attatcgaca tcggcgggaa ttgctttaga gaatatggat attatgtttt
     6661 tagctttttc attatcttca atgattactt cttttcatat tagtggtacg caagcgggtt
     6721 tcatttcaac gatcactaat ttggggatgc tcattggagg tatttttttt ggcatcatag
     6781 ctgataaata tggacgtgtg aaagtatttt ctcaaacggt ccttctgttt tcgattgctt
     6841 ctttattgat gtattttgct tcaaacatct acttggttta tctctttcgc tttatcgcag
     6901 gtgtaggagc gggaggagaa tacggggcgt gtatgtcctt gatttccgaa agtttttcaa
     6961 aaaaacaaat tggacgagct tcttcggttg ctggtattgg tgcgcaggtc ggtgcggctt
     7021 tggctgcgat tttagctgct gtgatcattc caacgtttgg ttggaagatg ttgtatgtcg
     7081 taggtgtact gccggtgttg atggtcttgt tcattcgtcg cggcttgact gaaccaaagg
     7141 aattcaaatc tgtacaatct gccgaaaaga aaaccaaact tagcagactg tttgaatcaa
     7201 aacatcttgc ttggcaaaca tgtggcctta gtatcatggt aactgtccaa attgccggct
     7261 attttgggtt aatgaattgg ctgccttcaa ttatgcaaaa tcgattaggg ctttcggttt
     7321 ctgggtcttc attgtggatg gtcagtacta ttttaggcat gtctttgggg atgatgactt
     7381 ttggcataat catggataaa ttggggccta aactagcctt ttctttgttt ttaatttgtt
     7441 catccttatc tgtctatcta cttgtgttag cccatagcca gtggagtttg gtagcagccg
     7501 ctgtgatagt cggctacttt atcaatggca tgtatggtgg atatggcgca gtgattagta
     7561 gtttgtatcc aacagaaatc cgcgcgacgg cgaataattt cattatgaac cttggacgag
     7621 ctgttggtgg tttttcttct atcgtcatcg gtttcttgat ggatcattat agtttaactg
     7681 ccgtaatctt gtttttgagc ggtatttacc tagtcagtct gctggtcttg acaactctta
     7741 ctggtgttac agcattaagg gaaagcttgc agcattgata gttgagtctg taaatgacaa
     7801 gaaagaggtg tggcaaaagc ttgcatctgc ggtaataaga ccagccaaat caaaaacatg
     7861 agacactgtt tccgtttggc tggtcgtatt acttgatgca gggcagcttt ttcaactgct
     7921 aatttccttc tatctggcgg ctactattct ttgacgtccg tcatttcaaa gtactttact
     7981 aataacttag cataacgaaa aactgtggat acactcgtat attgagcgtc tgcataatct
     8041 tccaaatttt tctcaggatg ttgatacatg aattcaatca aatgataatt atatgatttt
     8101 ctgtagaaat gaagataaat aatgtagatt gggaagttct cctgcttttc catagaaaag
     8161 cgccgattat tctcattctt caaaatgacc ttatcaggat atttttggaa caattcttct
     8221 aaatctgtta gataattcaa gatagactta cttgatacct tcaattcgtt ttgtagttct
     8281 tctattgaac accaacgttc tgtttcaagc agtaaatgaa tcattttaat tttcttttga
     8341 gaaacttttg ttaaaaaagt ctgcatattt tctcctcctg tatctacttt actatactac
     8401 atacaagaaa aaaaacggat acaaatatca tattgtcaca ccgtcaatag tgcataaacc
     8461 tgttattatt tctctttttt tcctaagtat tctattgata tgaatcattt aatcgtttat
     8521 tatataaatg tcaataatct aataataaaa taacgt
//
LOCUS       DAYYKN010000115         8493 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000115 DAYYKN010000000
VERSION     DAYYKN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.117"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACHBB7_002280"
     CDS             complement(<1..149)
                     /locus_tag="ACHBB7_002280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9210191.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACHBB7_002281"
     CDS             complement(593..1714)
                     /locus_tag="ACHBB7_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFM9210192.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACHBB7_002282"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACHBB7_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFM9210193.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACHBB7_002283"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACHBB7_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFM9210194.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACHBB7_002284"
     CDS             complement(4304..5215)
                     /locus_tag="ACHBB7_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9210195.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACHBB7_002285"
     CDS             complement(5232..6239)
                     /locus_tag="ACHBB7_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFM9210196.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSIPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACHBB7_002286"
     CDS             complement(6236..8365)
                     /locus_tag="ACHBB7_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9210197.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACHBB7_002287"
     CDS             complement(8370..8480)
                     /locus_tag="ACHBB7_002287"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210198.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1727 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggaattga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DAYYKN010000116         8262 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000116 DAYYKN010000000
VERSION     DAYYKN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.118"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..759
                     /locus_tag="ACHBB7_002288"
     CDS             <1..759
                     /locus_tag="ACHBB7_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9210199.1"
                     /translation="KKKIGDEISPEAVQKEFILRDSFAKNQFQQLMADIPAEEIEWVK
                     QVVELAEDKLKVEFSPNIYLTLTDHLHYAITRAKENIQLPNPLLFETKKFYPKEYQAA
                     KEALLFVKQKTGVELSEDEAGFIAFHFVNSQQGNEDMQVTMTATTMVRDILSIISKFF
                     GIVFDEESLNYQRIITHLQFFAQRYLKGEGSDEQDEFLYALVQGKYPKAFHCVERINE
                     YLRQTQKQEMGIAEQIYLTIHIQRVVSEKKSIQS"
     gene            880..2745
                     /locus_tag="ACHBB7_002289"
     CDS             880..2745
                     /locus_tag="ACHBB7_002289"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9210200.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            2759..4202
                     /locus_tag="ACHBB7_002290"
                     /pseudo
     CDS             2759..4202
                     /locus_tag="ACHBB7_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            4310..5011
                     /locus_tag="ACHBB7_002291"
     CDS             4310..5011
                     /locus_tag="ACHBB7_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9210201.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            5137..5649
                     /locus_tag="ACHBB7_002292"
                     /pseudo
     CDS             5137..5649
                     /locus_tag="ACHBB7_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(6047..6256)
                     /locus_tag="ACHBB7_002293"
     CDS             complement(6047..6256)
                     /locus_tag="ACHBB7_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM9210202.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            6497..7072
                     /locus_tag="ACHBB7_002294"
     CDS             6497..7072
                     /locus_tag="ACHBB7_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM9210203.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            7072..7512
                     /locus_tag="ACHBB7_002295"
     CDS             7072..7512
                     /locus_tag="ACHBB7_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM9210204.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            7528..8229
                     /locus_tag="ACHBB7_002296"
     CDS             7528..8229
                     /locus_tag="ACHBB7_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9210205.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         2711 a         1371 c         1827 g         2353 t
ORIGIN      
        1 aaaaaaaaga ttggggacga gatttcgcca gaagcggtcc aaaaagaatt tattttaaga
       61 gattcttttg ctaaaaacca attccagcag ctgatggctg atattccggc agaggagatc
      121 gaatgggtca aacaagtagt cgaactggct gaagataaac taaaagttga attttcgcct
      181 aatatttatc tgacattgac cgatcatctt cattatgcga tcacacgagc aaaagaaaat
      241 atccaactgc ctaatccatt attatttgaa acaaaaaaat tttatccgaa agaatatcaa
      301 gcagcgaaag aggctctttt gttcgtgaaa caaaagacgg gtgtggaact ttctgaggat
      361 gaagccggtt ttattgcctt tcattttgtg aatagccaac aaggaaacga agacatgcaa
      421 gtgacgatga ccgctaccac aatggtaaga gatatactga gtatcatcag taaattcttc
      481 ggcatcgtgt tcgatgaaga atctttgaat tatcagcgta tcattactca tctgcagttt
      541 tttgcccagc gttatctcaa aggggaagga tctgacgagc aagatgaatt tctctacgca
      601 ttagttcaag gaaagtatcc aaaagcgttt cattgtgtcg aacggatcaa tgagtatctt
      661 cgacaaacgc aaaaacagga aatggggatc gcagagcaga tttatttaac gattcatatt
      721 caaagagtag taagtgagaa aaaaagcatc caatcataag gattgttact gtatggcagg
      781 caaaacctgg attgaaaaaa taggttcacg aatgtttgtg actgtttttt tcaatctggg
      841 tttttatttt ttaaataaaa aagaaagaag gaaaaaacaa tggataatca agcagttggt
      901 cgtcgtgtat gggaagcggt aggaggacaa aagaatgtca aaagtttagt acattgtgct
      961 acgcggttgc gattcagatt aaaagatgaa tctttagcag atacgcaaaa gttaaaagaa
     1021 gacccggacg tgatccaagt cgtacaaagt ggcggtcagt atcaagtagt gattggcagt
     1081 aatgtagcag atgtttatca atcgatcgtg gatgaggaag gtcttgcagt ttcggaggat
     1141 ggaaaagaag agtccaaaaa tattttgaat cgtctgatcg atattatctc tagtatattt
     1201 acaccttttc taggggcgat ggcagccgcc ggggtattga aaggattctt atcattggca
     1261 acggtgatgg gctggctgac agctgatagc ggtacttatc aaatattgtt tgctgcagca
     1321 gatggtgtct ttaccttctt acctgtcatg cttgctttta cagcagctaa aaagttcaaa
     1381 gccaaccagt ttttagcagt agcaattgct atggcacttg tgtatccagc tatcacagca
     1441 gttgctggtg cgggagaagc tatcagtttc tttgggatcc cagtgattct ttcaccatct
     1501 ggttatactt cttctgtcat accaatcatt ctagccgtat gggttcaaag taaattagag
     1561 ccattcgtaa aaaaagtgat tccgcaattt ctgcaaatga ttttagttcc gcttgtcgtt
     1621 ttagtagtga tggttccatt aacatttctt gctttaggac caatcgggac ggttgccggt
     1681 aatgctttag gcggtctctt caactcgatt tatggattta gtccaatcgt agcaggtcta
     1741 atcatgggaa gtttatggca agtattcgtg atgttcggca tgcattgggg attcgtaccg
     1801 atcatgtttt tgaatatcga acaatatggg tttgatgtat tgatgccaat gcttcttcca
     1861 gccatcttgg cacaaggtgg ggctgcttta gctgtagcat tgcgaacaaa agatacgaaa
     1921 cttcgagcat tagggatttc ttctacagtg acttcgttat ttggaatcac tgaaccaact
     1981 gtgtatggtg tgacattacc tttgaagaaa ccattcattg ccgcttgtat ttctggaggt
     2041 attggtggag ctattatcgg attttccgga gtaaaagctt tctccagcag tttggtcagc
     2101 ttattgacga ttccgacttt cattaatacg gtggatggtg tagagtcaaa tgtgacagta
     2161 gcagtaatag ctacaggaat tgcttttgtt cttgcattcg taggtacatt gattttagga
     2221 tttgacgaac aaacacaaga caatcaatta gaaaacaaac atgcgaatgc aggagaaccg
     2281 attacttcag cacggcatac cttaaaaagt cctttaacgg gtaaagtact gccattgagt
     2341 gaagtaccag accaagtttt ttcttctggc gtgatgggaa aaggtatcgc tattgatcca
     2401 gaagtaggag aactagttgc acctgcagac ggagagatca cgactatttt tccaactgga
     2461 catgctgtag ggatcacaac gactgacgga gcggagattt tgattcatat cgggatggat
     2521 acagtagaat taaatgggaa tggctttgag atcttagtca aacaagggga tctcgtcaaa
     2581 gcaggagatt tattgattcg ttttgacatc gaagcaatta gagcagctgg ctatagcgta
     2641 atcactccag ttgtcattac gaatactgat gcatttgccg atatacttga attagaccaa
     2701 aaagagatta ttgcaaacga agatgttctg gcaattgtaa aataaggagg aaatcaggat
     2761 gcaatcacgt ttttcagaaa aatttttatg gggtggagca actgctgcca aatcaattag
     2821 aaggggctta tgatcgagac ggaaaaggac tatccgttgc agatgctatg cctggaggta
     2881 agcagcgctt tgcgattatc ggcagtgaag aatttgactg gacgattgat caagaaaaat
     2941 atatttaccc gaatcatcga ggaatcgatc attacgatcg tttcaaagaa gatcttgcct
     3001 tgtttgcgga aatgggattc aaatgttatc gtttttcgat tgcctggaca cgaatttttc
     3061 caaatggaga cgaaggtact cctaacgaag cgggattaga attttatgat cagctgattg
     3121 atgagtgctt aaaatatgat attgaacctg tgatcacgat ttctcattac gaaatgcctc
     3181 ttcatttggc aaaagaatac ggtggctgga aaaatcgaaa actgatcgac ttctatgaac
     3241 gatttgctca gacagtatta gagagataca gcagtaaagt caaatattgg atgactttca
     3301 atgaaatcaa ttcggccttt cactttccag ctttaagtca aggattagta aaaagcaatg
     3361 gagcaggaga gtaccaaaat attttccaag catggcataa tcaattcgtt gcaagtagca
     3421 aagccgtaaa aatcggccac gaattgcgtt cggatatcca aatcggctgc atgattatct
     3481 atgcaacaac ttacagcatc gatgccaatc cagtcaatca ggcagcaaca atgatccaaa
     3541 accaagaatt caattttttc tgcacggatg tccaagtacg tggagaatac cctgcataca
     3601 cagcgcgtac ccataaaaaa tatggagtcg atccggaaaa attagagcaa acggaaaaag
     3661 acttccagtt gctaaaagaa tatccagtgg actacatcgg atttagttat tatatgtcta
     3721 cggcaatcaa tgagactgat ccacaagcag caacttcaga agggaacttg ctgggaggag
     3781 taaaaaatcc gtttcttgag gcaagtgaat ggggctggca gatcgatcca gaagggctga
     3841 gaatcgcatt gaatgaatta tacaatcgtt accagaaacc attattcatt gttgaaaacg
     3901 gtcttggagc cattgatcat atggaagagg atggcacgat tattgatgat tatcggatcg
     3961 attatttacg tcgtcatatc gaagcgatgg ctaatgcagt agcagacggt gtagatttga
     4021 tgggctatac gccttgggga tgtatcgatc ttgtcagtgc atcaacagga gaaatgagta
     4081 aacgttacgg ctttatctat gtggatctag acgatgaagg aaatgggaca ctgaatcgat
     4141 caaagaaaaa atcttttgat tggtataaag aagtaattgc ctcaaatggc gtaaacttgt
     4201 aattaaagca gctgaatgaa aaagaacaca cttctcacat gtgatttgtg ttcttttttt
     4261 attttttatg tgctattctt ttagtaatag taaagcaaag ggtgacgtta tgaaaaggac
     4321 acgtgtacta tacttggaag tcgcagataa aattaaagaa gatattttct caggaaaata
     4381 tccagtcggt tcgatgttgc cgacagaaag tgaattagaa gaactgtttc aagtcagcaa
     4441 aatcactgtc cgaaaggcaa tcgaactgct ggcatcagat gaatatgtcg caaagaaaag
     4501 tggtcgtggg acgacggttc taagcaatcg tccttacaat cgattatcca aagcggcaag
     4561 ctttacgcaa atcttggaga actcccgata tgaagtccgg aaagaaacgc tggatttgca
     4621 attggtccat ataaagaagg aagatccttt gttttccttt ttcggtgagc aagctgtttg
     4681 ttttaagcgc ttatattatt tgaacaatca gccttatatt tattttgttc attatcttcc
     4741 acaagaaatg atgcaattca gcagagaaga atttggcaaa gaatctttgt atcgtttgct
     4801 gacgaaaaaa ggatatacta ttgactcatt ttctgatgat ttctcggcag tatttttaac
     4861 agaagaagaa caaggaattc tgaaaacaga cgcacagatc ggcatcaaac ggatacggaa
     4921 atcgtctacg caaattgggg aaatgattga atattcggag gcggtctacc atacggagct
     4981 gcatccttac catattgatt atgaaacata aataaaaaaa gaggttgtga aaaagctgcc
     5041 ctgcatcaag taataagaac agccaaacaa aaatagcttc tcatattttt cgtttggtta
     5101 ggcttattac cgcagataca cggctgtttc tggattacca tttattctgg ataagggggt
     5161 gtggcacgat tttcagaagg aatctatgaa ccaatcactg tattgatttt agcttcgatg
     5221 gtaaccccat tcggcttgac gatcgcttat ttcttaggga aaatcattcg taaaaatata
     5281 ctcaatcgtc aagaaatcga tacattgaag accgcttttc ctatggggat ctgtcagatc
     5341 acagagggat gtttccctat cgtattgaac gacttggcac gcaatgtcat agcaacggga
     5401 gtcggtggga cagtaggtgg cggattaagc atgttttggg gcgcagatag ccgtattccg
     5461 gcatcgggaa tgtttgcagt cgctacgatg accagaccat gggcattcat tggtgcattg
     5521 ttagctggtt cttttgtcac aggaataacc attttattat tgaaaaaacc agtagatccg
     5581 aatgcagaaa tcatccaaga agaaaaagag gaagaagaca tatcttggga tgatttgaca
     5641 attagttagg agaggcagaa tggattacaa gaaaaaagta caatagatgt accttgagac
     5701 aggaatggta tgaaagaatc agagttagaa agaatcgaat atacagattt tggtttagga
     5761 gcgatcgaaa ctgtccagag catcggcacc ctccttgaaa tgacgatgaa ggaaaaatga
     5821 gaacctttcg ttgtcgaaag ggtctggtat accttatgtc gaaggaaagg aaacaaaaaa
     5881 actttcctgt actattccaa aaaggaaaga aagttattat acagtaaaaa aagaaattat
     5941 tcttcatgct agagatcagt acacgttcga gcccaatatc aaacactagt tccaggcagg
     6001 cgcaacaggt gcggttatca ctgaattttc ttccagcagt gacgattcaa gtgatatttt
     6061 tacaaatcca gctatttatc gttaacaaaa agatttttag gagaagctga tgaacaaaaa
     6121 tgatcaaatt ttgatcgttt agttcgtcgg cttttttatt tagagaaaca tcatttatca
     6181 gtgaaacata acacgagaac aaaatgattt tttcgttaat aaaagaaaca agtatttgtt
     6241 gtttaaaata atacacgtta ttggcatata ggacgaaatc ccagtatctt ctataagtaa
     6301 gtcatttcat atcgatgaaa acacgttatt tatttttgtt ttatctataa aatggctgaa
     6361 aaatgatttt tttctaaata ctatatatag tggcgtctat cctatatacc actaaaaaac
     6421 tacaaaatct agtcttttat tttaaaaaaa cacgtgtaga atgaagatag acttttgagt
     6481 tagggagtgt ttttttatga aacttactgt tttttgcggt tcccgttttg gtaataaaga
     6541 aagttacaaa tttattgcgc aaacgttagg aaaatacatg gcacaagaag aaatcgaact
     6601 ggtttacggt ggttcatact ccgggattat gggagtgatt agtaaaacag tactggaaaa
     6661 taacggaaaa gtcacaggta tctatccgaa tgggcttttt gaaactgaat tgcctggaaa
     6721 agatgttact acatttattc ctacagaaac gatcgatgaa agaaaggtat tattatttga
     6781 aaagggagat gctgtcctcg ttttccctgg aggtctaggt acattggagg aattctcaca
     6841 acttctttct tggatagcaa tcggtctgac gcccgacaag ccaatcggaa tattgaatat
     6901 tggcggttat tacagcggct tgcaacaatt attagaaact tttgcaaaag aggattttat
     6961 ggataaaaaa tggttggatc aagtaatctt ttccaacaat ccattggaac tggtcaatga
     7021 attacgagca gtatctatta aaaatcagtt attattgaag gaggcaaaat aatgagtcaa
     7081 atatatttag caggaccatt tttttcagag gagcaaatcg atcgagtaag tcgtatcgaa
     7141 aaagcgttgg aggaaaacaa aacagtcaca agtttctact ctccacgtca tcaccaagaa
     7201 agcaattatg aactattcag cgctggatgg gcgcaggaag tatacgaaaa agatatggaa
     7261 gaactgacaa atgcagaatt tgttgttgct atccttgact ttgagcatca aacaatcgat
     7321 ccaggaacgg cttacgaatt aggtgtggca actatgttga aaaaaccgat gatcattgta
     7381 caagaagaga cggtacctac gaacttgatg attacacaaa gtctccatac gtatttaaaa
     7441 tcagaccaag ctgtacgtga atatgatttt gaaacattgc ctgttgaaac ttacgtaggc
     7501 gagtacttat agaagaggaa gggttgtatg tcagtgattt tgatttcagg aacgattggt
     7561 gcaggtaaga gcagtttgac ggatatgctt gcaaaagaga ttgattctaa accattttat
     7621 gaaaatgtcg aagataacga agtattgccg ctcttttatt caaatcctga gcagtacgct
     7681 tttttattgc aaatcttctt tttgaacaag cgatttttag caatgaaaaa tgctttagtg
     7741 aacgatgaca atgtgctaga ccgatccatc tatgaagata gcctgttgtt ccatctaaac
     7801 gctgatttag gtcgggtaac ggatatcgaa gtacagcagt acgatagctt attagatacg
     7861 atgctaaacg agttagatga tgttgctcca aaaaaacggc ctgatttgat ggtccatatc
     7921 aaagtttctt tggacacgat gctagaacgt atcaaaaaac gtgggcgtga ctatgaacag
     7981 ctagaaagcg atgagacgtt atatacctat tatgaaacgt tgaacacccg ctataatcaa
     8041 tggtatgaag attttgatgt atgtcctaaa atacaagtgg atggggataa attcgatttt
     8101 gtagaaaatc cagaagatgc aaaaaaggtg atccgtcaga tccaagaaaa attagctgaa
     8161 ttggaaggaa agcctgctgg aaaatatttt acggataacc agcatgcaaa aggtcaagaa
     8221 ttaggctaaa aataaaaaac caaacgacgg gaaagaaaaa tt
//
LOCUS       DAYYKN010000117         8248 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000117 DAYYKN010000000
VERSION     DAYYKN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.119"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="ACHBB7_002297"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="ACHBB7_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM9210206.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="ACHBB7_002298"
     CDS             complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="ACHBB7_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM9210207.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="ACHBB7_002299"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="ACHBB7_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM9210208.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="ACHBB7_002300"
     CDS             complement(join(3472..4500,4502..4573))
                     /gene="prfB"
                     /locus_tag="ACHBB7_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM9210209.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="ACHBB7_002301"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="ACHBB7_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM9210210.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="ACHBB7_002302"
     CDS             complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="ACHBB7_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM9210211.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8134..>8248)
                     /locus_tag="ACHBB7_002303"
     CDS             complement(8134..>8248)
                     /locus_tag="ACHBB7_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFM9210212.1"
                     /translation="KILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
BASE COUNT         2200 a         1816 c         1440 g         2792 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaactc aaaaaaactt gcggcagctg tcagtagctg ctgcaagttt tcgttgttat
     7441 ttaagtaagg ctgatcagtc tgtttcaatc agtccgtatt ttccatcttt acggcggtaa
     7501 acgatacttg taccatttgt ttctgcatct tcaaaaataa agaagttatg tcccagcatg
     7561 ttcatttgta agactgcttc ttcactatcc attggtttca atgaaaggcg tttcgtacgt
     7621 acgatatcta attcagaatc attttcgttt tcgtcctctt cattgaataa agcagcagca
     7681 tctgcagtat tcatgcccgt ttcacgggat ttacgattaa tttttgtttt gaatttgcgg
     7741 atttggcgtt ccaatttgtc aacaaccaaa tcgatacttg cgtataaatc tggagaagtt
     7801 tcttcggcac gtaatacaag ataaggtaat gggatcgtta cttccacttt tgctgttttt
     7861 tctgtataaa cttttaagtt cacatgcgcc gttgcatcgg gtgcgtcact gaaatagcgt
     7921 tctagtttgc ctactttttt ctcaacgtag tcgcgaatcg cttcagttac ttcgatattt
     7981 tcgccgcgta cattatatct aaacataaaa attacccctt tcgtctacca caatcaagaa
     8041 ggagaaggac cactctttcc ctcttcttaa ttctattata tcgaactcta ggttatttgc
     8101 aaattaaatc gctatcaaat ttccacatct gattcatctt gccaaagaaa tggttcggat
     8161 gattttcggc tgtgcgttct gcaagcaatc tgctgcatga aaaagcgtct gtcctgttgt
     8221 atagacatcg tcaataatca atattttt
//
LOCUS       DAYYKN010000118         8135 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000118 DAYYKN010000000
VERSION     DAYYKN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.120"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1009)
                     /locus_tag="ACHBB7_002304"
     CDS             complement(41..1009)
                     /locus_tag="ACHBB7_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM9210213.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1241..2074
                     /locus_tag="ACHBB7_002305"
     CDS             1241..2074
                     /locus_tag="ACHBB7_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM9210214.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2108..2947
                     /locus_tag="ACHBB7_002306"
     CDS             2108..2947
                     /locus_tag="ACHBB7_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9210215.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            2973..3533
                     /locus_tag="ACHBB7_002307"
     CDS             2973..3533
                     /locus_tag="ACHBB7_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9210216.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3732..5453
                     /locus_tag="ACHBB7_002308"
     CDS             3732..5453
                     /locus_tag="ACHBB7_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM9210217.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5618..6760
                     /locus_tag="ACHBB7_002309"
     CDS             5618..6760
                     /locus_tag="ACHBB7_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9210218.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6776..7987
                     /gene="thiI"
                     /locus_tag="ACHBB7_002310"
     CDS             6776..7987
                     /gene="thiI"
                     /locus_tag="ACHBB7_002310"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM9210219.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2788 a         1422 c         1692 g         2233 t
ORIGIN      
        1 acagcctctc tttttaagtt tagctattag ataatgatgt ttagttttgg ataaacgaaa
       61 tcaattcttc acccgacaaa ccatagttta cagcaatcaa tagttttaaa cgtgcttttt
      121 gactattaat actgttacag aaaataatcc ccattttttc tagttctttt cccccgcctt
      181 cataatcata aacaggctct gcgattccat tgaaacaacg agaaacaagt actacaggga
      241 ttttttggac aagaagcttt tccaaagcag ctaaggcttg tggagggaga ttccccgcac
      301 ctaaagcttc gattaccaaa ccgtctactt tcgtatgggc gatggcttcc aataagtccc
      361 cttgcatccc agcatatgct ttaacgatcg ggatagtccc atctacagcg gaaatgtcca
      421 gtcgctttgt ttctaaaagt tcctgcaaga ataaaattcg attcttagtt actaagccga
      481 tgggacccaa tgtaggtgtt cggaaagtag caacatttgt ggtatgggtc tttgtcacgt
      541 agcgtgcaga atggatctca tcgttcatga ccaccaaaac ccccttatct agcgcttctt
      601 cacaacttgc gacacgaatc gcactttcaa aattataaag gccgtcactt ccaagttcat
      661 tacttgaacg cattgcccca gtcaaaacaa ttggtaattt gttgccaatc gttgtatcaa
      721 gaaaaaaagc agtttcttct aatgtatctg taccgtgtgt gatcaccact ccggatatac
      781 ctgatgcata agcggacaag atacgttctt ttaataccaa catgtgagaa ggcatgatgt
      841 gtggtgaggg aagattgaag atatcttcaa ctaataattc tacatgcttg gggattgtga
      901 tttctgcatc taataatggg tttgccacat caggactaac accgcccgtg agcatgtttt
      961 ctttcatggc gatcgttccg ccggtgtgta gtacaagtat ttttttcatt ttacagactt
     1021 ccttacgaat aattttgcat agccgttaaa tcttattgta tactatataa aagagttttg
     1081 ctatcagtga agaataacga atagcctaac ttatcataaa aaagctgacc ttattaaaat
     1141 ggaaaagact caaccttgac ctcctaatac aatggagtat ctggttgagt ttcttttaca
     1201 agaaagcagc caaggaaatc aatcacgaag gtggccggaa atgatcagaa tcggattaac
     1261 atcgtttaaa gaacatgaca aattaactgg taaaaagaat aacactcttt atgaatatgc
     1321 agggtttcta cccttagtgg aattagatac tgcctattat ggaattcccc gaaagtcctc
     1381 agttgagaat tggataagtg aagtacctga atcatttcgc tttgtagtca aagtctatca
     1441 aggaatcagc ggtcaaggag actggaagcc ttattatcag accgaagaag agatgattgc
     1501 gcatttttta gatgttatgt cgccaatggt agatgctgga aagatgtttt gctttttagt
     1561 ccaattcccc tcatcattca aatgtacgaa agaaaatgtc cagtacttgc gcaaacttag
     1621 aaagtggttt gagggttatc ctgtttctat agaattgcgg gattattctt ggtacagcga
     1681 tgaatttctt gaacgtacaa gaaactttat gtctagagcc gctttttctt tagccatcat
     1741 cgatgagcca cagttgctga atacaacgat tccttttgat ccatttgtca ccaatccgaa
     1801 ctttgctctt tttcgattcc atggtaggaa tgcgcaagga tggcaagcaa atgataaaga
     1861 gtggcgtaaa aaaagaacgt tatatcgtta ttcagaaaaa gaactgcaag aactagcaca
     1921 tgaagtcaaa gtagttgatt ctaagacaaa agaggtaggt gtgattttta ataataattc
     1981 tggtggtgat gcggctgaaa atgcattagc actgcaaaaa atattaaata tcaagccaga
     2041 tggactcaat ccaacccaaa tggatctgtt ttaagggctt attttattca agaaaggggc
     2101 gaagtctatg atcaaagcta ttttttttga tattgacgga acattggtca ccaatcgttc
     2161 aaaagcgttg gaatcaacaa aacaagcaat tgaatatgct cggaaaaacg gcattttatg
     2221 cggtgtagca acggggcgca gcccagtaaa gatcaaagaa atcattgatg aattggaatt
     2281 agatatgtat gtcgtgtata atggtcaact ggtttttact gcagatcgca cgattatcga
     2341 ccaccctttt gaacaaaaag tattggaaca tattgttgaa tttgcagatg agaaccatcg
     2401 acaaatcgtt tttggtgcga gaaatcggtt agatggatca acgacgatgc tattaggaca
     2461 atcgattttt atcaagcgac tagtcagctt tttaccgcgt aaattcccgg tacgtctgat
     2521 gaaaaaaatc ctccaggttt ttagccctca ccgacaaaag gatcgttatg aaacgttaga
     2581 gatattaaaa gaaccaattt atcaatgtat attgctcagt ccggagtcag aacagaaaaa
     2641 gttagaacat cgctttcccg agtgtacgtt tcagcgttca aatacgtata ctgtagatat
     2701 catcccaaaa ggtggttcaa aattactagg catccaagct ttcgcaaatg ctgtcgaaat
     2761 tgaaatggaa gaaattatgg cattcggcga tcattacaat gatatcgaga tgctgaaagg
     2821 tgtaggaatt ggagtggcca tggggaatgc tcagatcgaa gtaaaacatg ctgcagactt
     2881 tgtgactcaa tcaaatgaag aagatgggat atacgaagga ctgaaacact tcggtctgat
     2941 ttattagtca atgaaagaag gaaaaaatca gtatggaagc agagacacct tttcaaaaag
     3001 tagaaacatt ccacaaaact tttgatccaa gaagaccaga gatacccacg gcttttacag
     3061 aaaatgaagc tcatacacgt gctgcgttca aaatagaaga acttgttgaa tttgttgcag
     3121 ctacttcacc taataaagaa caactgcatg aagctgttat gctgctgcac gaagcattag
     3181 ataaagcaga agaaaaaata cttggtaaaa cacagtgggg aagtgatccg ctggttgaac
     3241 aagtagatgc actgacagat atcctttact ttacttatgg ttctttttca ttactaggaa
     3301 tagtgcctga tcgaatattc gaaatcgtcc atcaggcaaa tatggggaaa ttatttccag
     3361 atggaaaacc tcattatgac cctgtaacaa ataaagtgtt gaaacctgaa aactggcaga
     3421 tggaatatgc tccagaacct aaaatcgcgg cagaaatcca acgtcagatt caagaagcga
     3481 ttgctaagaa gaaatccgct gatataggca tgtgcacaaa tgaaaaagag tgatcagagt
     3541 ttcactattt ttgttatctt tatacgatgg aaaaacaaaa gattttcgtt taagttagtg
     3601 aatgcatgaa aatagtatgc tataatctag aatagatgtc ttttgcacga gagacgtatt
     3661 atttggattt atgccaagca gaacgccatc tgttgcataa aagatgcata ctgaaaacgg
     3721 aggaaccata aatgggaaat aatgttatca tcggaatcgt cattgcaatc attatagtcg
     3781 ccattgtcgt ctttttggtt agttacttga tgaagaaaaa aaaccaagat cgattggatg
     3841 ccttagaaaa acgcaaggaa gaattatttg acttgccagt gattgaagaa gtcgatgatg
     3901 ttaaaaaaat gcatctggtt gggcaaagcc aaaatacctt tcgtgaatgg agtcaaaaat
     3961 ggaatgacat atctacttcc tcgttcgctg aactagaaag tcaaattttc gaagtggaaa
     4021 acctcaatga aagttttcgg ttcttaaaag gaaaaaaagc agttgttgaa gcagaagcta
     4081 caatggacca aatggagaaa caagtgacgg aaatccgtga aggtctaaag gaattgcggg
     4141 aaacggaaga acgcaactca ctagctgttc agcaggcact tgacgtctat gaagaactga
     4201 aagaaaaagt cagagaagac gagcatgaat acggaccagc tctttttgaa ttgcaaaaac
     4261 agatcaaagt gatcgaaaaa gaatttacgc agtttgtgac tttaaacact tctggagacc
     4321 ctgttgaagc ccgcgaggtt ttggataaag cagaaaaaca tacgtatgaa gtggaagctc
     4381 tgatgaaaaa aattccaccg ctgtatgagg agctccatac cactttccct gaacaacttg
     4441 aagaaatcag tgatacttac gagaaattga tggaagaaca atatgtattc ccagaagaag
     4501 atcttgctga agatatcgca aaagtaagtc gaagaattga aaattcatta gccaatcttg
     4561 aaaaaacaga agttgaaacg gtcgaatttg aaaaccgtga aacagctgat ttgattgatt
     4621 cgttatatga tatcttagaa cgggaaatgg aagctcagag atatgtaaaa actaaccaat
     4681 caacgattgc agagtatatc aaacatacaa caaagaataa ccgtcaatta ttgattgagc
     4741 tggatcacac atctcaaagc tatacattga accacaatga aattggccgt gtacgaggtt
     4801 tccaaaccga agtggaagaa atggaacgtc aaaatgaaca gatgattcca cagatccgtc
     4861 aacacgaaat tccttattca gaagtgcgca cgttctataa aacagtcttc aaagtcttag
     4921 aagatatcga aaagcaacaa gtagagatcg acgactcatt acatgaattg agaaaaggcg
     4981 aaaaagaagc gcaagaaaag atcgatacat tcgaattcaa attgcgcagt ttgaaacgtt
     5041 ttgtagaaaa acaacgtttg cctggcttgc cgaatgatta cctagaattc ttttttgttg
     5101 ctactgaccg tattgaagaa ctaagtgttg tcttgaataa aattcgagta aacatggaag
     5161 aggtcaatcg tttagttgct ctatgtgaag aagatctaga attattagat aagaagacac
     5221 atgatttagt agatgcagca gcattaacgg agcaaatgct ccaatatgca aatcgttatc
     5281 gtcatacaca cgaagaagtg agagaagcaa ttgataaaag ctactattta ttcaataaag
     5341 aatatcacta tcaagaagca ttagacgaaa tcggtacagc attagagcga gtagaacctg
     5401 gtgctttcaa acgcatcgaa gatttttatt ttaatcatcc agacttagta taaagcaaaa
     5461 aagttagtaa accagcaaga gcaagacgga ttattgtctt gttcttgtct ttttttgtat
     5521 atttgaaaat tgaaacaagt gaaaatttca cctgctcttg aaagcacctt ggacaaccgt
     5581 tataatagaa aagaatttca aaaaagaggg gtttcttatg atatattttg ataacagtgc
     5641 aacaacccca atctttccgc aaagcttaga tgcatatgta aaaaccagtc agcggattat
     5701 tgggaatcca tcaagcttgc atgatttagg caatcaagct aaccggttgt tgcagcaagc
     5761 aagaaagcag atcgctgatt tgatccatgt agaaccagaa gaaatctatt ttacttcagg
     5821 cggaacagaa ggagataact gggtattaaa aggaacgatg atcgaaaaaa gagaatatgg
     5881 gaatcatatg atcatttcag gagtcgaaca tccggccgtc agcgagacag cagaacaatt
     5941 aaaagaatta ggaatcgaag taagtatcgc cccagtggat aaacgcggat ttgtacgtgt
     6001 cgacgaattg aaagaattaa taagaaaaga gacagtatta gtctctgtta tggctgtgaa
     6061 taatgaagta ggatcgattc agcccatcca ggaaatcagt gatcttttgg atgattttcc
     6121 taaaattcat ttccatgtag atgctgttca ggcgattgga aaagtaccat tctcgcaatg
     6181 gttgacagaa agagttgatt ttgcaacatt ttcagctcat aaattccatg gcccacgggg
     6241 aactggtttc atttattgga aaaagggtcg tcgtttagca ccattactga caggtggagg
     6301 gcaagaaaga aatcagcgaa gtgggacaga aaatgttcca ggtattgtgg ctatggttaa
     6361 agctttacgt ttatcattag agataaagga acaaaaacct gagcagacac aacgcttaaa
     6421 agtagctcta atcaatgcgt tgaagacgta tgagaaagtg acgatttttt cagaaggaac
     6481 tgactatgca ccgcatattt tatgttttgc tctaaaagga attcgcggcg aagttctcgt
     6541 ccatgcctta gaagaaaaac aaatatttgt ttctactact agtgcctgct cctcacgtaa
     6601 acatatggca agcagtacat tgcacgcaat gcatgtacca aatgatctgg ctacatctgc
     6661 tgttcgaatc agcttagatg aaaacaattc gcttgcagaa gtcgagcaat ttatgattat
     6721 tttcaagcag ctttataaga aattttctgc aatcaattaa aagaggagtg ttcttgtgaa
     6781 atacagtgaa attatggtcc gttatgggga actttcgaca aaaggaaaga atcgaaaaag
     6841 ctttatcatg caactagcac aaaatgtcag acaagctttg aaagactttc cagaaatcaa
     6901 aatccatgct gatcgggacc ggatgcatct tttattgaat ggcgcagacg ggcagctagt
     6961 tattccaaaa ctagcaaaaa tatttgggat ccaaaatttc tctccaagta ttcgtgtaga
     7021 aaaagatgtc gaagttttga aacaagctgt tcaagacatc atgaaagaaa tttatactgg
     7081 gaaagaaaca tttaaaatta tcgctaaaag aagcgatcac caatttgaat tgacaagtaa
     7141 tgaattgaat caaacattag gtaatgctgt ttttgatatt ttcccgcata tccaagttca
     7201 aatgcgtcaa ccagatattc cattacgtgt agagattcgg cgtgatggcg cttatctatc
     7261 gtatgaaacg atcaagggag ctggaggatt gcccgtagga acaagcggaa aaggaatgct
     7321 tatgctttct ggggggattg attctcctgt agcagggtac tttgccatga agcggggagt
     7381 ggaaattgaa gctgttcatt ttgctagtcc gccgtataca agtgaacaag cactgcaaaa
     7441 agcaaaagat ctaacagcca aactagcacc ttatgtggga acgatccatt ttatcgaagt
     7501 gccatttacc gagattcaag aagaaatcaa aaagtcagtg ccacaaggat attggatgac
     7561 aattactcgt cgaatgatgt tgcgtttgac cgatgaaatt cgccgtattc gtcatggttt
     7621 agtcattttg aatggtgaat cattaggtca agtggcttca caaactttgc aaagtatggt
     7681 ggctatcaat gaagtaacga atacgccaat cattcggcct gtagcgacaa tggataaact
     7741 tgaaatcatt gaagtagcag aacagatcga tacatttaat ctttctatcc agccttttga
     7801 agattgctgt acgatttttg ctcctccaca gccaaaaaca cgccctcaac tggaaaaggt
     7861 ccatcaatat gaagagcgtt tagcaataga tgaaatgatc caacgagcac tagctggctt
     7921 gaagatggaa gagatacaag cagaatcgtc taatgaaaaa actgaagaat tttcagaatt
     7981 gctataagag gttatgaaaa tagtgttttg accagagtat tagagaaaca aaacgaaaaa
     8041 atagctccat gtattttacc gtttttgaac taatgtcgaa gattagctga ttgaatacca
     8101 tttatcaaga agttatggaa aagccggttc ttttt
//
LOCUS       DAYYKN010000119         8134 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000119 DAYYKN010000000
VERSION     DAYYKN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.121"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..141)
                     /locus_tag="ACHBB7_002311"
     CDS             complement(<1..141)
                     /locus_tag="ACHBB7_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFM9210220.1"
                     /translation="MILIVKYRVEVLFMVTRIFILDDSLVFEKMLVEILEESRILLSP
                     WKF"
     gene            complement(350..1186)
                     /locus_tag="ACHBB7_002312"
     CDS             complement(350..1186)
                     /locus_tag="ACHBB7_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM9210221.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1197..2126)
                     /locus_tag="ACHBB7_002313"
     CDS             complement(1197..2126)
                     /locus_tag="ACHBB7_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM9210222.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2318..2671
                     /locus_tag="ACHBB7_002314"
     CDS             2318..2671
                     /locus_tag="ACHBB7_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9210223.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2745..3401)
                     /locus_tag="ACHBB7_002315"
     CDS             complement(2745..3401)
                     /locus_tag="ACHBB7_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM9210224.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3627..5561
                     /locus_tag="ACHBB7_002316"
     CDS             3627..5561
                     /locus_tag="ACHBB7_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9210225.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5647..6870)
                     /locus_tag="ACHBB7_002317"
     CDS             complement(5647..6870)
                     /locus_tag="ACHBB7_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9210226.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(6864..>8134)
                     /locus_tag="ACHBB7_002318"
     CDS             complement(6864..>8134)
                     /locus_tag="ACHBB7_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM9210227.1"
                     /translation="PFAFHYYGLIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGA
                     NVPFGRSMIYRFAVSSFWSAMLWADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILN
                     IGYTYPQLTLTEPYNSTLSPLWLNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEA
                     NLLIQHDNGHTFFLNAGQLGPNFHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDS
                     MLGIQRADTYITTSFKNESIKTYGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWL
                     IPFEGWQIRVHKVELDTEYVLYETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIIC
                     LSDDTIKRKSTAIMGLPNTNLMTWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLE
                     VWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
BASE COUNT         2598 a         1642 c         1373 g         2521 t
ORIGIN      
        1 aaatttccaa ggacttaata aaattctgga ttcttccaaa atttctacta gcattttttc
       61 aaaaaccaaa ctatcatcca aaataaatat acgcgttacc ataaaaagta cctccactcg
      121 atattttact atcaaaatca tttcatacaa cttttcaaca tacatgttag gaataaattg
      181 taattctctt cacaatcttt tccaacacct ttgaaaccga tatcaaatac tttcaggatg
      241 ctgcttctaa aataaatatc aaacaaatga atacatcatg caaaaaaagc cagacaaccg
      301 acgtccttgc gtgtcagctg tctgagctat gtttatttat tttgttcttt taaaaagaac
      361 gatacacata ctgatttttc ggagcagaga ctttctcagc gtttgtaaca actacagatg
      421 ggatttcacg cttgaatggt tgataatagt ctgattggat ggttccttct attttatacc
      481 attcatcatt tttaaccgtt actccttccg gcatatgcgt caacaaacca aagactcctg
      541 aatctgccac acaatggata atgccgaatc ggaacaagaa gatatctgta ttgctatcgt
      601 tcggtgtttg gtaaacaaat ccttcatagc tgattttttt gccaatgaat tcgctagggt
      661 aattataaat tagctccata acttctaaat agttttcatc tgtgatcttg atagtttgct
      721 tatccccata cttttctttc aactcattca tttggtctaa gtagtctgat ttgttgaaat
      781 aaatactggt atccggcttt aagtattgtg tctgcatctc cggatcccct actgattctt
      841 tacttactgg gaaattaaat cctttggctt ctacgatcgt agtgtcgaga ctaactgttg
      901 gaaacaacgt ccccacgatt actggcaaag ctagcagtac ataagcgatt ccacgttgat
      961 aaggttttga caacccatgg tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt
     1021 ttttgaccca caaatacagc tgaactaaag ccaaaagagc agataaaatc attgaaagaa
     1081 tagctaagta gcgataatgg acattgatat attgatcgag tttgccagaa acttgcaagt
     1141 acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt tccctcctaa
     1201 ataaataatg tgaatccaat aacgctaagg gcaatcagtc cgatcagcga taaaataaag
     1261 cgtcctgtaa aatatcgttt catcatcagt agatttttga tatcgaccat tgggccaaag
     1321 accaaaaagg cagctacagg tgctgtgccg aacagactta acaaagaaga tcctataaac
     1381 gcatctgctt ctgagcaaag agacatcgta aaggccaata acagcatgat gataatggct
     1441 aaaatctttg ttgagccgag cgttagcata attctagttg gtaaataagt ctgcatcgca
     1501 gctgcgatta atccgccaat gatcaaataa cgacctgtat caaaaaattc gtcgattgaa
     1561 tgtgtcaaca catgcccagt ttgatggatc agctgatttt ttggttcatt cttctcactt
     1621 tgttcttctt catggacatg atgatggtga ttttctgcta ctttagacgt gattttttct
     1681 ttcaatacag cgtctttttg aaaataggca agccagacac caatcacaag ggcaacaaaa
     1741 aagctgccta tcacccgcca gcttgccatc tgccaagaat tgccaaatgc gataaaagtg
     1801 gaaaaaatca cgattggatt gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga
     1861 actggtacgc cttttttaac aaattgatga acgatcggta cgatgccgca ttcacatgaa
     1921 ggaaagaaaa atcctaagat cgaaccagta aaaatagaga agatacgatt ttcaggaagc
     1981 catctcttca cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat aagacagcct
     2041 aataatacga aaggtaatgc ttcaataacg atcgataaga aaatcgtact catctgcaaa
     2101 accgaatttg gaataaaaga aaacatgagt ttcctccttt ttatttctag ttcattcgct
     2161 ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc
     2221 ttatttttta ccaaagcact tcaattgact ttttctgtta ttcccattaa attaaggtct
     2281 catacatatc acttctatca taaaagggag aataaaaatg aaacaaacca ctaacttgac
     2341 tgaagttcaa gacatactgc aacagtcatc cgttgccgtc atttatcttt cgatgcctaa
     2401 ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa cttctcacac attatgatat
     2461 tccggcactt catttggatg catttgaaac accagaagta gctagtaaat ttgaagtgct
     2521 gactgcgcca gttgtcctga tttttcatca aggaaaagaa gtctttcggc aagcacggtt
     2581 catcgacttt aaaaaaatcc gttatctact agatgaactg acagcagaag atgattccct
     2641 ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa aagcgattga
     2701 acaagtgaaa gttttgcctt catttgttca atcgcttttt atttttattc tgctgtattt
     2761 tcggaatgcg catttgcgtc agaatgcaag aaatagatca aggtttgcaa ttcattcgtc
     2821 aaatcaacat tttggatcaa tacatctgac ggagcattca atcgagcagc ggtaaagttc
     2881 aagattcctt tgattcctgc ttccgttaac tgattgacta cttcttgtgc tctttcagcg
     2941 ggtatcgtta aaattgctac ttcgatttgt tggatcttga tttgttctga catatcttcc
     3001 attggataaa cggggatacc gtctacgatg cgcccaacga tttcctcatt cacatcaaaa
     3061 gcacaactga tacgtatgct attactttga tggaatttgt attttagtaa agcacttccc
     3121 aagttcccta caccgatcag cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca
     3181 aaaaagtgca taagatcttc tacatcataa ccatacccac gttttcctaa ttcgccaaaa
     3241 taagaaaaat ctcgacggat cgttgcacta tctacttgaa cggcttcact taattcagtt
     3301 gaagacactt ttttctttcc agcattattt aaaatcctta aatagcgata atacaaaggc
     3361 agtcttttag ctgtcgcctt tggaataata tgatctttca cgaaatgacc tccacgtatt
     3421 gtctaattac atctttgatt tctccatcat agcagtattt ataggaaaaa atcaattcca
     3481 ttgctcagat attgtgaaat tttattcttt attcatttat ttatcatttc tgcaaatctc
     3541 gcagtataat ggaatttatg gtagactact aattgaaaaa tagtttttta tttgaggaga
     3601 attcacaaag tagaaaagag gattccatga tattattaca agccaatcag attgctcgcc
     3661 attttggtgc agaagtctta tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg
     3721 tcgctttagt tggcagaaat ggcgctggaa aatcgacttt attaaagatc attgctggaa
     3781 ttgaagcacc tgatgaagga aagatcgcta aaaataaaac agcgactctc ggatacctcg
     3841 ctcaggacac aggacttgat tccaatgaaa cagtttggaa cgagatgctg aaagcttttg
     3901 aacaagttag acaaatggaa gagcggatgc gcgagctaga gattttcatt agcgaaggta
     3961 cgccagacac acctgcttat agttcttatt taaaagaata cgaccggctg caacacgatt
     4021 tttcagatcg aaacggctat ggttatgaaa atgaaattcg ttccgtattg catgggttta
     4081 aattcgattc ttcattttat gatcagaaaa tcgatacttt atcaggcgga cagcgcacac
     4141 gtttagcttt agctcgcatg cttttgcaac accctgacat tttgattcta gatgaaccaa
     4201 cgaaccattt agatatcgag acactttcct ggcttgagaa ttatctgcaa agctatactg
     4261 gtgcattatt gattgtttcc catgaccgtt attttttaga taaagtagtc aatgaagtct
     4321 atgaattaag cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa tatctagatt
     4381 tgaaagcaga acagttagca agtgaatgga aggcttatga aaagcagcaa acagaaatcc
     4441 ataaattaga agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc
     4501 aaagccgcag aaagacatta gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa
     4561 aacaagcaaa ctttctattc agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg
     4621 aagatgccgc tatcggatat gataatgtca ccttatctga accaatcaat ctagatatcc
     4681 gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat cgggaaatcg acgttgctga
     4741 aatcaatcat tcaccagatc ccttttatcc gtggaaaaga aacgctaggg acaaatgtca
     4801 ccatcggcta ttacgatcaa ggtcaagctg atctgcatac aaacaagacg atcctagcag
     4861 aattgtggga tgagcatcct accactccag aaaaagagat ccgaagtgct ttaggtgctt
     4921 tcttattttc tggcgaagat gtagaaaaac caatccctct gctaagtggc ggtgaaaaag
     4981 cacgggttgc tttagccaag ttagcgatga acaaagaaaa ttttttgatt ttggatgaac
     5041 caacaaacca tttagatatt gacaacaaag aagtattaga aaatgcattg atcgattatg
     5101 acggcacact gctttttgtt tcccatgacc gatatttcat caatcggatc gcgacaaaag
     5161 tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg agactatgat tattatttag
     5221 agaaaaagca ggaagaagaa gaaatcgccg aattgttggc acaagatgag ccagaatctg
     5281 ctcctgctcc cacgaacaaa tctgattatt accaaagcaa agaacagcaa aagcttatcc
     5341 gtagccttca acgaaaaatt actcaggttg aagaagaaat ggcgcggatc gatgaatgga
     5401 tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac gtaaaactca
     5461 atgagctgaa ccaagattta gaagctgcta gacaagatca agaaacaaaa ttgacggaat
     5521 gggaagaact aagcttggaa ttagaagaat atgaaaatta acgaaaaaac caaaacagaa
     5581 accattcatg ctgttttggt ttttcttttt ttattttatc aaacaaaaat tccatattta
     5641 cctttttcac caataagaag accaattact agtcattctc actaaagctt ctaaataata
     5701 gtaatcaccc caaatacaag gtgtatttac cccattattt ttgggcttat tataaacaga
     5761 ctgtgttaaa aagccatctt cttcttccgt tgtagcatat ctttctttca attgtcgcat
     5821 aattttccaa acagcagact ggtataagtt cttttctgtt ttgtctaata gacctgattc
     5881 tattaattct aataatccac aaaccatgat caccatacta gatgtatctt tttcttctct
     5941 gctagaatca tcaaagatca aatcccaata acaaatggta tcttctggca accgattcat
     6001 aaaataattg gctaatattt tgcttaaatc aaaatatttt tgttctttca aataacgata
     6061 acttaatgca aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg aattatcact
     6121 ataaccttga tgcgtacttc catatcttgg ttttccatct ttgacatcca tataaaatgt
     6181 atgataagtt gaaccatctt ctcttactaa ataatctgct gcattttgaa tatgagcctg
     6241 agcagctttt ttatattttt gatttccagt ttcttcactc gcccaataca ataatggaag
     6301 attcatgcaa caatccatga tcattctacc ttgttgtgaa ggatcactag gatctcccca
     6361 agcttgtata actcctgcct tctcccaaaa acgctgaagt aatgtttctg cagcatcaat
     6421 tgaagcttcc ttcatttttc gatttcccgt tattttataa tctgctacgg ttgataaaga
     6481 atacaaaaaa ccaagatcgt gatgattagt gataatttga cagtcaatcc tgtggataaa
     6541 atctatatct gctttttcag ctgtttgttg aaataaagga tcacccgtct tctcaaataa
     6601 caaccaatta attcctgtcc aaaatccttc tgtccaacca atattgtctc ttaatggata
     6661 acgtcctttt ataggacact cgcttgggaa ttgatactga ctgttactga tacgtttgat
     6721 taacctctcc gtgtgtctgc gacaacgctc gatctcatta ttccattctt tttccattaa
     6781 ttctttataa cattcattta tccatttttc tggatgtatt atctcttcaa acatcacttt
     6841 atcaagcatt tccccaccag cctttagcat aattttagtc tttttttatt gtttttattt
     6901 tggatagtta gcccggttgt cccatcaaaa tcaattttgg gacgattgtt ccatacttct
     6961 aaactataat cccgatcttg atgagcaacc actcctgttc cgagccaatg ggttcctttt
     7021 ccaaatgtac cctctaatcc aggaataaaa gtgttttccc atgtcatcaa gtttgtattg
     7081 ggcaaaccca taatagctgt ggattttcgt ttaatagtat cgtcacttaa acagataata
     7141 cctgaaaagc cagcttcact caccctatag ttttccttgt cttcttctat gattattcct
     7201 tctttctcag gagaacattc aatagcaaac cctgtctcat atagaacata ttctgtatct
     7261 aattctactt tgtgtacgcg aatctgccag ccttcaaaag gaataagcca agttctgatt
     7321 tttgcctgta aagtaggttc ccatgtagag gcaagtacct ctttcttaca tacaatgtct
     7381 cgtacattat ttcgaacgat gaactgtcca taggttttga tgctctcatt cttaaaactg
     7441 gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg aatccattgc catttctgct
     7501 ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc aaattttaaa
     7561 tatttttcat tggtcaatgt atggaaattt ggccctaatt gtcccgcatt caaaaaaaat
     7621 gtatgtccat tatcatgttg tatgagcaaa ttggcttctg aaagaagttt agttccttca
     7681 cgaataggca taggcatttc tttagccgtc caatactcat ggtcttcagg tagcgccaac
     7741 aataaaaaaa tcttattcag ccataatgga gataacgtac tattataggg ttcagtcaat
     7801 gtcaattgag ggtaggtata accaatattc aggatacctt tttcatcaaa tatttctttt
     7861 tgcatccacc atcttatatt ccgattgact aaccccttga tttcacctaa tttaaaaggc
     7921 aaaagatcgg cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg
     7981 cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa aatgaatata gtctctagaa
     8041 aaatcaatag ctcggttctt atatatctgg ctaatgggct tttctggttc tagtacgcta
     8101 taaatcaaac cataataatg aaatgcaaaa ggat
//
LOCUS       DAYYKN010000120         8131 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000120 DAYYKN010000000
VERSION     DAYYKN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.122"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            382..570
                     /locus_tag="ACHBB7_002319"
     CDS             382..570
                     /locus_tag="ACHBB7_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM9210228.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            648..1661
                     /locus_tag="ACHBB7_002320"
     CDS             648..1661
                     /locus_tag="ACHBB7_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9210229.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1728..2762)
                     /locus_tag="ACHBB7_002321"
     CDS             complement(1728..2762)
                     /locus_tag="ACHBB7_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9210230.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2877..5102
                     /locus_tag="ACHBB7_002322"
     CDS             2877..5102
                     /locus_tag="ACHBB7_002322"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9210231.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5120..7003
                     /locus_tag="ACHBB7_002323"
     CDS             5120..7003
                     /locus_tag="ACHBB7_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM9210232.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6984..7958
                     /locus_tag="ACHBB7_002324"
     CDS             6984..7958
                     /locus_tag="ACHBB7_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM9210233.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2632 a         1479 c         1711 g         2309 t
ORIGIN      
        1 gttagtctac attattttgg cgatttgcca ataaggactg ttgagttgtg caaaatcaaa
       61 tctgggtcag aatgaacgtc attctgtctc agatttttcg ttatttcaat ttgattaact
      121 gattgatcaa aaagattttt attttcatat tctcaaatga ttcaacacta aatatacatg
      181 tttgacgcgg aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga
      241 cccattattt tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg
      301 ctgggagttt tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg
      361 actgtaacga aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa
      421 gcgacacgat atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca
      481 acagaagaag aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca
      541 ttgcagaaga agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac
      601 actatggtat aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa
      661 ttcttaaatt tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc
      721 ttgccttcca gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata
      781 ctgttcaaat cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa
      841 cagatttgac gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa
      901 acagcaaggg gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag
      961 ataaatcggg tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg
     1021 ttgcgatgaa ttatgaatta gcgcctgatt cagaataccc taatcaagtt cttcaagtga
     1081 atgaactgat caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac
     1141 aattattttt tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac
     1201 aaacgaatga tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc
     1261 tgaaaggaac catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt
     1321 cggatgatcg atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa
     1381 aagattggaa aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg
     1441 aatttccgcc aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag
     1501 cattagaaaa tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata
     1561 caaaaaaaga gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt
     1621 gttaccagca gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt
     1681 ttcgttttga gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt
     1741 gttcttggga taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt
     1801 tcgtcccgta taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat
     1861 ttcgcattga tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta
     1921 tcaaagcttg ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga
     1981 tagacaccaa ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca
     2041 ttttctaata gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga
     2101 taagacatat atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc
     2161 ttttcattta tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg
     2221 atataggcta ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc
     2281 cgttcaagat ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg
     2341 actatttgca tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca
     2401 acgatcaccg cacctaactg atctagatct tcccattgtt taggattatc agatagatta
     2461 tagattcgat tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa
     2521 tgtttgtaat agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc
     2581 gaaggcattc tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct
     2641 gcctctacta tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac
     2701 acacgggaaa ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaacc
     2761 atttttacac actcctcaaa aaaataaaaa atttttattg acaaaattgt ttagtaaaca
     2821 gtatacagga attataaaaa aaagtaaacg gtttccgttt tgttgggagt gaaatgatgg
     2881 atattacgca tagaaaaaca gataaaaatt tcatcttatt tgatgaaaag agccgagtct
     2941 tccatttacg aaatacattt ctttcttatc tgataaagat agaagaaagc aatgtattgg
     3001 cgcatattta ttttggcaaa ccagtcaagc agtacaaaga caataaaaat tatccccgaa
     3061 gagatcgtgg attttcagga aatgttccct taaatcctga ccggtctctg tcgaaagata
     3121 cactaccgca agaatactct tctcatggca gcatggactt tcgaacgccc gcatcgatca
     3181 ttcagcgcaa gaatggatca gacttactgg atctgagata tgacagccat tatattacag
     3241 atgggaagcc agagatcgaa ggcttgcctc aaacttatgt gatggacaag tcagaggcac
     3301 aaacgttggt gatttcactt aaggatagag aaacagcgat ttatttcgat ttgttctata
     3361 ccatatttac tgatagagca gtgatcacac gatctgtaaa gataagaaat gaaaccggag
     3421 aaacaatcaa actagaaaaa gcggctagtt tccagctaga ctttgcccat actaggcgat
     3481 ttgatgaagt cattgctttg ccaggagcac atgtcaatga aagacagatt tcacggcaat
     3541 ctgttttatc tggaacaaaa gtatttgaaa gccgtcgagg aacgagcagt caccatatga
     3601 ataactttat tgctttagtt catcatcata cgacagaaaa tactggagaa gcgatcggac
     3661 ttcaatttgt ttattccggc aaccattctt tcgaacttga aaaagatcag atcaatcaat
     3721 tgagagtcgt cggtgggatc aacagccatc gtttttcgtg ggaattgaat gcaggccagt
     3781 cttttcaaac acctgaaatg atcctttctt actcaagtca gggattgaac aaaatgagtc
     3841 agatccacca tgagttgcta cgtgaaagaa tcgcaagagg tcgtcatcaa tttgctgaac
     3901 ggccgattct ggtcaataac tgggaagcca cttactttga cttcaatagt gaaaaaatca
     3961 aggcgatcat tgatgaagca aaggagctag gcatcgaaat gttcgtatta gatgatggct
     4021 ggtttggaaa aagagatgcg gacaattctt cattaggaga ctggttcgag tatgaaggca
     4081 agttgacaaa tggattacgt gaaatagcag attatgctca tagcaaagga ttgaaatttg
     4141 gtttatggtt tgaacctgaa atgatctcgg ttgattctga actgtatcga acacatcctg
     4201 attttcttat gcaggagcca ggtagaatgc cttctgcttc cagatcacaa catgtactgg
     4261 attttacaag gttagatgtt cggcagacga ttgagaaaca aatgagaaag attttagata
     4321 cgattcctct tgactacatc aaatgggata tgaaccgttc gctttcagat gtctattcga
     4381 ttacccttga cccacagaga caaggagaag tagcgcatcg ttatatgctc gggctttatg
     4441 aactacttga acatttgaca actgattatc cggaaatctt atgggaaggc tgttctggtg
     4501 gaggtggacg gtttgatgcc gggtttattt attatatgcc gcagtcatgg acaagtgata
     4561 acacggatgc agtggagcgt atgaaaatcc aatatggaac aagccttgct tatccgattt
     4621 cgtccatcac agctcatgta tcagctgtac caaatcatca gaccgacaga tctacttcgt
     4681 tgaaaacaag aggagaaaca gctatgagcg ctgtttttgg ttatgaactg gatttgacaa
     4741 agctgtctcc tgaagaaaaa aagcaagtaa aagaccaaat tatatcctat caaacgattc
     4801 gtccggtgat ccagtatggc cattattatc gtttggcgag tccttttgaa gaaaatattg
     4861 ccgcatggat gtttgtgtct cctaaacaag atgaagctat tgtgttttta gggaggatac
     4921 tagcttcagc tcaaccagcg ttccatgaag tatatctgat ggggttagat gatgaggcac
     4981 tttatcagga acagacctcg aagcggatat tttcgggggc cgaattgatg acagttggac
     5041 tttacttccc cgattttcaa ggtgatttcc aaacagaact gcttcatttc aaaaagttat
     5101 gagagagaag gaaaaaagta tgaaagcaaa tataaaatgg ttagatgatc cacaagtttt
     5161 tagagtgaac caatcaaatg cacatagcga tcatgtttgt tatgcaacaa gggaagaagt
     5221 agaaaccaca tcctccctta aacagtcttt aaatggatca tggcgttttt gttattcgcc
     5281 aaatccttcg gtacgaccaa aaacatttta taaaaaagat tttgatgcct gttcttttga
     5341 tgaaatcact gttcctagtc atattgaatt agcaaattat gacaagatcc attacatcaa
     5401 tacgatgtat ccgtgggagg gacatgtatt tcgccgtccc cctttcacga caagccctga
     5461 gaaagaggcg gaagggatct tttcagctgc taaagacaat ccggttggtt cgtatttgac
     5521 gacttttcga ttagaagaag gtctaaaggg aaaacaaatc tcgattcatt ttgctggtgt
     5581 agaacaagca atgtttattt ggttgaatgg tcattttatc gggtatgccg aagattcgtt
     5641 cactccgtct gaatttgatt taacacctta tttggaagaa gaaaatattc tagctgtgga
     5701 ggtttacaaa agaagtacag ctgcattctt ggaagatcag gactttttcc gattttttgg
     5761 gatcttcaga gatgtcttct tagttgctcg aggacaaaac catttagaag atctttggat
     5821 caaacctcgc ttagatgaca aatgtcaaaa tggcaaactt gaagtagatt tgtcttttga
     5881 gcaagtagcc aaaaacagtc agctgaatgt agcgatacta gatcctgatg ggaaggaaat
     5941 ctatcaacag caatatccgt taaaagagca aatgacgatt cgcatcgatg gatttgaaaa
     6001 tgtggcactg tgggggcatt ttcagcccaa tttatataag cttgaattgg agacgataga
     6061 cgaagaaaaa agagtaacgg agtatattga ctattccttc ggttttcgta aaatcgaaat
     6121 caaagataaa atcatctact tcaatggaaa acggatgatt atcaacgggg taaatcgaca
     6181 tgagtggcat ccgaaaaaag gacgcgccat cactatagaa gatatggaaa aagatttggc
     6241 tatcataaaa aatgcaaaga tcaacggcgt acggacatct cattacccca accagatcac
     6301 ttggtattat ttatgcgacc aagcaggaat ccatgtgatg gcggaaacga acctcgaatc
     6361 ccatggctca tggcaaaaaa tgggcgcatt tgagccttct tacaacgtac caggtgcagt
     6421 acctcagtgg caggcggctg ttcttgatcg agcaaaaacg aatttcgaaa cattaaaaaa
     6481 ccatccaagt attctttttt ggtctcttgg aaatgaatct tatgcggaag aaaacattcg
     6541 acaaatgaac gattacttca aaaaagtaga ccccgatcga cttgtccatt atgaagggaa
     6601 ttttccaaat ccagcattcg aagattcgat ttcagatgta aaaagctgca tgtatgcgcc
     6661 accagaagag atcattgact accttgaaaa taaagcggaa aaaccgttca tcttatgtga
     6721 atatatgcat gacatgggga attcgttagg tggaatggat tcttacatga cgttgcttga
     6781 tcgttatgaa atgtaccaag gaggctatat atgggatttt aaagatcagg cgatccaagt
     6841 gactgatcca atcacaggca gagaagtgat gcgctatggc ggtgactttg acgataaacc
     6901 aagtgactat gaattctcag ggaatgggat cgtttttgca gatggacaag aaaaacccgc
     6961 catgcaggag gtaagatatt attatgaaaa atacagtaaa taaaagtcat atggatacgg
     7021 aaaaagttgc aatcgtcttc ggcgactgta cattaggtgt caaatcgggg aatacgcatt
     7081 atattttttc ttatacaaga ggcggactgg aatcgctcaa taaaaacgga aaagaatggc
     7141 tataccggga aacacttcca gttttttggc gagcaacgac cgataatgac agaggtagcg
     7201 gatttggatt taaatccggt atgtggttag gagccggatt gtatccaaaa gtcaaagaaa
     7261 tagaagtact ggtggacaat aaaaagatcg atttgccgat tgctccagta aataatcaat
     7321 attcaagtaa tgaatacgct gaaacggttg aaatcatttt tacactggaa atcaacacag
     7381 taccaaaaac ccaagtgaca atttcctacc aaatcgctgg agagggtaaa atgcttgtca
     7441 atatgcatta ttatggacaa aaaggattac cggaattacc cgcattgggt ttaaggttca
     7501 ttatgccaac gaaagcaaaa ttttttgagt atgaaggcct atcaggggaa acatatccgg
     7561 atcggatggc tggtggtgtc gaaggtattt ataaagtggc aggactcccc gtttccccat
     7621 accttgttcc ccaagaatgc ggcatgcata tggaaacgaa atgggtagaa ctaacaagaa
     7681 atcaaacgtt ggataatacg gataaagatc aagaagattt ttcgttgcga tttgaacagc
     7741 aggatacgcc atttgctttt tccgctttac cattcaaagc agaagaactg gaacaggcaa
     7801 cacatataga agaactaccg ccagaaagac gaacggttct tacgattttt gcaaaagtaa
     7861 gaggagtcgg gggtatcaat agctggggag cggatgttga gtcaccttac cgaatcagca
     7921 gcgaagagga ccatgaaatc ggctttatta ttcagtagca atctttagat tattatttga
     7981 aaaacggagt ttacaagaca aagctgcttt attctatatg tcatgaacaa gagagtaaaa
     8041 aagttctcag atacttttgt tcatgactat tttcttcaat aaaaccaatt gttatttttc
     8101 tgaaaaacaa agttaactat cagaaaatta a
//
LOCUS       DAYYKN010000121         8067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000121 DAYYKN010000000
VERSION     DAYYKN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.123"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHBB7_002325"
     CDS             476..1339
                     /locus_tag="ACHBB7_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9210234.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHBB7_002326"
     CDS             complement(1468..3090)
                     /locus_tag="ACHBB7_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9210235.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHBB7_002327"
     CDS             3339..3860
                     /locus_tag="ACHBB7_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM9210236.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHBB7_002328"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHBB7_002328"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM9210237.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHBB7_002329"
     CDS             5941..6759
                     /locus_tag="ACHBB7_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM9210238.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHBB7_002330"
     CDS             complement(6862..7059)
                     /locus_tag="ACHBB7_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210239.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHBB7_002331"
     CDS             7228..7806
                     /locus_tag="ACHBB7_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM9210240.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHBB7_002332"
     CDS             7901..8038
                     /locus_tag="ACHBB7_002332"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210241.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2408 a         1469 c         1592 g         2598 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacat cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatct
//
LOCUS       DAYYKN010000122         8016 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000122 DAYYKN010000000
VERSION     DAYYKN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.124"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..885)
                     /locus_tag="ACHBB7_002333"
     CDS             complement(37..885)
                     /locus_tag="ACHBB7_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9210242.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1109..3049)
                     /gene="thrS"
                     /locus_tag="ACHBB7_002334"
     CDS             complement(1109..3049)
                     /gene="thrS"
                     /locus_tag="ACHBB7_002334"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM9210243.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3386..3565)
                     /locus_tag="ACHBB7_002335"
     CDS             complement(3386..3565)
                     /locus_tag="ACHBB7_002335"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM9210244.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3612..5435)
                     /locus_tag="ACHBB7_002336"
     CDS             complement(3612..5435)
                     /locus_tag="ACHBB7_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9210245.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5713..7992
                     /gene="helD"
                     /locus_tag="ACHBB7_002337"
     CDS             5713..7992
                     /gene="helD"
                     /locus_tag="ACHBB7_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM9210246.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2352 a         1706 c         1449 g         2509 t
ORIGIN      
        1 tattctaaaa aacttatata aaaaatagta aggtttttat ttcgaatcca taaaatattc
       61 ttggattgct tctcgtgttt ttttcacttt ttcgctaagc ttttcgtttt tagccatata
      121 gaaataaaaa atgatatcga tcgtagcaaa ttgggccaaa agtgaattag ttgccgcact
      181 tcgattcgtg atttcttttg gtctagatgt ttgtacgagt acatcggcta tcttgctaag
      241 cgggttattt ccaaattgcg tcaacgcaat gatttcgata ttcatgcttt tcgccagctc
      301 ggctaatgcg actacatctg cacttctacc tgagtttgaa acaagccaaa gcactttctt
      361 tttctcattg ctgacaagtt gcgggagcaa gacgtggata tctttttcaa aaatgattgg
      421 cttaccgaca cgagaccatt tttgcagaat atcttctact actaaagaag aggcgccaac
      481 cccatataaa aaaacagtct ctgccgattc caaacactct acgacttttt ggatcgtttc
      541 ttcttctaga atatctactg tttcttttat cgctacttgt gcattgttac caagtttgtt
      601 tttgatagag taaaaggact catttgcttc tatatcacta aaaccttcat cgattccagt
      661 ctgtagttct gttgaaatcg tgattttgaa gtccgtcaga ctttgaaaac cgattttttt
      721 ggaaaaacgt acaactgtcg gcgcactcaa cccggaagct tctgctaatt cattagctgt
      781 cattgtaggg accttctcaa tattaccgat aatgtagttg gccaactttt tttctgctga
      841 agaataatct tccatcatac tttcaatacg actaattacg ctcataagtt cctccaccct
      901 tttaatgata gtctatgtaa gtataccata tcagaccctt attcacggtc gtatactgga
      961 taaaaaacaa aaaaacaatc cgcccaacag aatatcttat cccatcaatc ccacagattc
     1021 tattgagcat ctgtgtcttt gaggaatacc tgttatctat tgttcggatt gctttctatc
     1081 tactactttt ttacaggaaa tttacctatt agttttctct gctgtaattt ttgacttctg
     1141 cttccatagc atcaataaag atattcaaat cttctacagc tgtttcttta cttccataac
     1201 gacgtacgtt cactgttgca tcttccaatt ccttgtctcc aacaactaat tggtaaggga
     1261 tcttttgtgt ttgagaggca cgtatcttat agcccatttt ttcattacga tcatcaactt
     1321 ctacacgcaa ccccttcatt tgcaaacgtt ctttgatttc ataagcataa tcgccatgtg
     1381 catcaacaga aacagggatg attgttgctt gtataggtgc caaccatgtt ggaaaagcgc
     1441 ctttgtatac ttctgttaaa taagcgacaa aacgttccat tgtagaaaca attccgcggt
     1501 ggataactac tggacgatgc gtattttccc cgtcttctcc aacatatgtc aaatcaaagc
     1561 gctctggcaa taagaagtct aattgtatag ttgataatgt ttcttctgta ccaagagctg
     1621 ttttgacttg aacatctagt tttggtccgt aaaaggctgc ctcgccttct gcttcaaagt
     1681 aatctagttc taattcatcc atagcggctt tcagcattgc ttgggctttt tcccacattg
     1741 cgtcatcatc aaaatattta tctgtattgt ttggatcgcg atagctcaag cggaaacgat
     1801 aatctgtaat attgaagtca gcatatactg ccaccatcaa ttcaagcgta cgtttaaatt
     1861 catctttgat ttgatcagga cgaacaaatg tatgaccatc attcaacgtc atttctcgta
     1921 cacgttgtaa acctgacaaa gcacctgatt tttcatagcg atgcatcatt cctaattcag
     1981 cgatacgaat tggtaattca cgataagaat ggatcgtgtt tttgtagacc atcatgtgat
     2041 gagggcagtt cattgggcga agaacaagca tttcgccatc tcccatatcc attggcggga
     2101 acatgtcttc ttgataatga tcccagtgtc cagatgtttt gtacaactca acatctccca
     2161 ttattggtgt atatacatgt tggtaaccta gactaacttc tttgtctacg atataacgtt
     2221 cgatcgtgcg tcggattgtt gccccttttg gaagccagaa tggcaaacca gaacctactt
     2281 ctggggaaac catgaacaag tcaagttctt ttcctagttt acgatggtct ctttcttttg
     2341 cttcttcacg gagacggata tattctttca aagctttctt gtcaaaaaag gctgtcccgt
     2401 atacacgttg catcatttga ttgtttgagt ttccacgcca ataagcacct gccactgata
     2461 ataatttaaa tacttgtata cggccagtgg atggaacatg tggtccacga cacaaatcga
     2521 tgaattcgcc ttgttgataa gcagtaatca cttcttcttc tggcaattca ctaatcagtt
     2581 ctactttgta cggatcactt gcaaaaattt ctaatgcttc ctgttttgaa agaacacggc
     2641 gttcgatcgg cagattttct ttgacgattt tcatcatttc tgcttcgatt gccggcaaat
     2701 cttctgcagt tacttggttt tgtccattat ctgtatcgta gtaaaaccct gaatcgatag
     2761 ctggtccaac gccaaagtgg atatttggat agaggcgacg catggcttgg gccatcaaat
     2821 gagctgctga atgacggact aagccaagtg catcttcatg gtctggtgtg atgatctcaa
     2881 tacttgcatc ttcctcgatt ggacgagtca aatcaacgag ttttccgttc actttaccag
     2941 ctaaagcttt ttttgctaag ctttttgaaa tgctttcagc aattgccaat gttgttgttc
     3001 cagactcaaa ttctttgact gcaccatctg gaaaagtaat ttttatcata atagtttcct
     3061 ccttgaaatg ttttgattga tcagatcatc aagctgaaaa acaaaaaaat cctagcatct
     3121 cttgaaaaga gatactagga cgaaaagttc gtggttccac ctaatgttca aacgcaaatg
     3181 cgttcctcaa gcgttgtaac ggtccgaccg cccttgcttc aacaagggtt tcgaaagtgg
     3241 tcatcctttg gttttctcct ggaaaagttt cagcctagct tttcctctct tatcgaagac
     3301 tctcaaattc agttggcttt ctcatcaatc aattatgtgt ttatttaacc actaataaat
     3361 agaaaaatca agtgggaaac taaacttatt ttttccattc cccatttaca ccgtatgttc
     3421 cttttttcat ctcattgatg atgacatgga tgtgttcttt tggtgcctgc gtgtttttat
     3481 gaacagcttc cgtgatatct ttcatcatat tttctaattg ttccggactg cgaccttcta
     3541 ctaattcgac atgtacgaat ggcataatgc tctctcctct ttctgatcgt tcttttttca
     3601 actcgattct atcatactat gtttgtcttg ggtactgaat caggaaaaat ggctggatct
     3661 ttttttgctc tcaaggcact caaaatagag accgtatcca cgacttcttg caaaacagct
     3721 ccgaataatg cggggattag tcccgtactt gcaattaaca tcaaaaaaat acaaataaag
     3781 atgccaatca gtacagactg tttcgctatt ctcatcgtat ctcttgaaat cactactgct
     3841 tttgctacac gcaagagatc atctttcaag atgaccgcat ctgcactttc actagcagct
     3901 gtagaaccat gagcccccat tgcaataccg atatctgcag cagcaagtgc tggtgcgtca
     3961 ttcacgccgt ctcctaccat cgcaacaggt ctttgatctt tagggatact ttctaaaatg
     4021 gtcagtttat cttgaggcaa gctacgggca tgtacagaat ctaattggac tttttctgca
     4081 atcgtttgag caatggcaga tccatccccg gtgatcatca tcaaattttt cacatcaagt
     4141 tttcttagct ccttgattgt ctttgctgct tctgggcgaa tctgatccga aaataggatc
     4201 gttcctgcaa aagatcgatc aattgttaca tagattgctg tattggacgt ttctatttct
     4261 tcagctgaaa caaaagaggc acgtccaatc tttacttcat gattattgac tactgcttgg
     4321 attccttgac cggtcacttc tttcagttga ctaacaggat acagttggct gtttttcacg
     4381 ttgttcacta acgaacgagc taagatatgg ctagattcct gttctgcact agctgcatac
     4441 agaagtagct cttctttttt tattccattg actggctgaa tcgcctctac ttccaatatc
     4501 cctttggtaa gcgttcctgt tttatcaaaa gcaatcgttt ttgtacgtgc gagcttttca
     4561 atcgttgttc cagtttttac aacgatcccg tttttacttg aacggctcat tcctgcaact
     4621 aaagcaatgg gcgcagctag aatcagtgga caaggtgatg ccactaccag tacctcagca
     4681 aatcgtaaag ggtcttttga gaaccaccaa gaaataccac cgatcaaata agcaatcaac
     4741 gtgaaaggga ctgcataacg atcagctaaa cggacaaatc gggcaggctt ttcttttgat
     4801 tcttcgacta gtttaataat catttgatac tggctgtctg cggcacgttt ttcagcgatc
     4861 atttggatcg atccgtctcc atttaaagac cctgacataa tctgatctcc aggtttcttg
     4921 gtttccgggc gggactcacc tgtcaaagaa gattcatcga ccgaagattc tcctttggtc
     4981 actcgaccat ctgcgggtac aagttcgcca ggcttgacta gtaactggtc atcaatattt
     5041 atttcatcga ttactatatc ctgcaatttt ccttctttga atcgatgggc aattcttggg
     5101 gtgttatcta acaaagactg cagttcttta cccgctcgtt tattcgcgta atcctctaga
     5161 ctatctccgc ccgtaagcat gatcaatatc atcaaactag cccagtattc tttgacggct
     5221 aatgttgcaa ggattgcggt aatcgctaat acatctacgc catatcttcc tgaacgtaac
     5281 gtcttgatca tttccaaaaa catcaagaag gcgagaatcc ctccagtaaa tgcgacaatc
     5341 gcaaatgcaa gggccggctg gtgaaaaaca aattcactta ataaagctat cccaccaata
     5401 agcaaagtaa gtaaaaattt tttgaaatgt gtcattgttt tttctcctct tttatgtgac
     5461 ttagagaggt tagtaataaa tttccagctg cctctttcat cttaagcata gtccttgact
     5521 agcacttttt aaaacaatag gccttaattg aaaacaacta tcactatcag cgtttccaaa
     5581 attcatcagt tctacaaaca aaaatattca gcaaggatta ctttttcgat tcatcagtta
     5641 aaaagtacct tttttcgaaa aactatgcta tacttttaat tcgttattta cggcggaagg
     5701 agcaaacatg cgatgaatga gaaacaacta gaacaaacgc acgtggatga aacgatcgaa
     5761 ctaatccaga tcgaacaaaa tatgctggat acaaaacaac aagaattgac tcaggaaatg
     5821 aatcattcga caaaagacac agctaatcac aagattcgag ctggttccaa tgaagctttt
     5881 tatgaatctg ctgttgaata tcgccaacat gagcaggaat tgcttttgaa ataccatact
     5941 cttgaagcac agcaaaaacg cctgcaaacg ttgtctgtca tgaaagatag tccttatttt
     6001 gcccgtatcg actttaaaga agaccgtgaa acagaaacat tatatctagg tatcgcttct
     6061 ttaagagatc tggcagaaga aacaatcgtt attgactggc gcgcaccaat tgccaatctt
     6121 tattatgaag gtgagattgg tcccgcattt tatgaaactg aaacagatag aatagatgtc
     6181 gatctgcagt tgaaacgaca atttaaaatc caagaaggac gtattttatc tatggtagat
     6241 acgtcggaag taatcaatga tgactttttg ctagagatat tggatgaatc atcaagcaac
     6301 catatgaaaa acatcgtctc tacgatacaa aaagcacaaa atgcaatcat tcgggacaca
     6361 acaagtaaag taatgctgat tgaaggaatc gccggaagtg gaaagacatc cgctttgctt
     6421 caacgtattg cttttctgtt gtatcataat cgcaagtggc tcgaggctga aaacgttctt
     6481 ctattttcac ccaaccattt gttttctgat tacatctcga ctgtattgcc ttcattaggt
     6541 gaaagcggcg tgcctacaca gacattcaaa aattatttgg tgcagctgct gccagagttt
     6601 tctttactag atgaacagca acaagaaagt ggttttttgc taggggaaaa agatccgata
     6661 caagtgatga aaagcggatt gaccttagtc gatcagattg atcgctatat ccaaggcatc
     6721 acaaactacg gtcccttgtt ccgtgatatg aaaataaatg gccgtacgat cctatcaaag
     6781 gaatcgattc gccaatggta taaagaaaca aatgaacaat tacctttaca tcatagactt
     6841 tctctattac aaacgaagct cctgaaaaaa ttaggcggtc tgcaaaaaga cgaaacacgt
     6901 cagcaatggg tcaaagatct ggcggaagaa cagctccaag aactatacgc aacagatcca
     6961 aatcttgaat atacagaaca aaaggagcgt tcattacgta aaaaactggc caaccaaatc
     7021 gtacgtaaaa gattccgtcg aatccatcga gggatcatga attatcaatt tgtcaatata
     7081 acgaaacaat atctccactt tctacaaact gtgccacttt ctcttctaac acgacataaa
     7141 atcagcgaga ccaggtggca ggaacatttg acaactgttc gtacacactt gcgtcaaaga
     7201 gaattgcgtc aagaggatgc tgtactattc tttttactga tgcgccgaac atatccagtg
     7261 acggttacac acaaagctcg ctatatcttt attgatgaaa tgcaggattt cgcaccatca
     7321 caagtggcat tgctgcaagc cttgttccca aaagcaaatt tgactttctg tggagatcta
     7381 aatcaaaatg tctttggaaa tgaaacgatc gttggatcat tggaagacct tttcgtggac
     7441 cgtgaagtca ctcgtttcca attaacgaca agctatcgct ccacaaaaga aatcactgat
     7501 tttgctaatc attttattgt gaaagaacat caagtagaaa caacagcaag aaaaggagac
     7561 cttcctttgc tcatccaagg aacaacagat gatatcaaat tgaattggtt agcagatacc
     7621 attcgaacga ctcagggaaa agcccggtat tggcgtacag caatcatagc taaaacacaa
     7681 gaagaatgcg aagctcttta tgctcaactg ccagacgagt tgaaacaaca agtccagctg
     7741 attttaaatg agagtgactt tatgaagcgt tccattattt tacttccagc ctttttagca
     7801 aaaggtctgg agtttgaccg agttttcttg tgggatgtag atgcagaaca tttttcttct
     7861 cagcaagaca aattgatcct ttacaccatg tgtacacgtg cgatgcatga attgaccctt
     7921 tttacctcca ataatgaaag tcctttgtta aacaatatgg atatttcatc ttatgagaaa
     7981 aaagtactgt aagtaaaaag acaactgcta tcatta
//
LOCUS       DAYYKN010000123         7958 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000123 DAYYKN010000000
VERSION     DAYYKN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.125"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..1641
                     /locus_tag="ACHBB7_002338"
     CDS             4..1641
                     /locus_tag="ACHBB7_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210247.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1786..2526)
                     /gene="truA"
                     /locus_tag="ACHBB7_002339"
     CDS             complement(1786..2526)
                     /gene="truA"
                     /locus_tag="ACHBB7_002339"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM9210248.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2717..3352)
                     /locus_tag="ACHBB7_002340"
     CDS             complement(2717..3352)
                     /locus_tag="ACHBB7_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM9210249.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3499..4719)
                     /gene="sstT"
                     /locus_tag="ACHBB7_002341"
     CDS             complement(3499..4719)
                     /gene="sstT"
                     /locus_tag="ACHBB7_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM9210250.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4938..5300
                     /locus_tag="ACHBB7_002342"
     CDS             4938..5300
                     /locus_tag="ACHBB7_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210251.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFLRTSWQLEYS"
     gene            5287..>7958
                     /locus_tag="ACHBB7_002343"
     CDS             5287..>7958
                     /locus_tag="ACHBB7_002343"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9210252.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2604 a         1492 c         1235 g         2627 t
ORIGIN      
        1 aaaatgaaaa aaaacaaaaa ccaaataata gatatcattt ttatgttatt tctaagtttt
       61 gcttacgtca tccctctatt catgtctatg ggaatctatc atagtgtcaa tcaggatact
      121 tactttcatt tatctcgaat cataggacta gataatgtat ggagttctcc agtaaacttc
      181 aataacttcg atcatcatgg aacaatgatg aatatttttt atccatggct tactctttat
      241 ccagcttttt tattttataa aatgatggga aatcttgttt taagttataa tatctactat
      301 ttttttatca cttttttaac aatggtcgtt tcttattttt ctatgaaaca aataaaaaac
      361 aatcgttata tttcgctgtt attttcgatt atttatacat tttccgccta tcgtgccatt
      421 gacatattcc atcgtgcttc attgggagaa gctgtcgctt taacattttt gcctttgata
      481 ctaatggggt gttacgaaat ttatattaga gattaccaaa aatggtactg gttgtctatt
      541 ggcatgacgc tagtagtgta tactcattta ctttctgtcg ctatggtttc tgtatttatt
      601 ggaggaacgt tgtttctttc tttttatttt tgggaccaaa aaatcgctag actcttaagt
      661 cttttaaaag ctaccgtcct cacctttttt ttaagtgcgg gattcttaat tccttttatt
      721 caacaatcta gagcacaaga attgaaagtc ccactgggaa aagaattaag tggtatggct
      781 cctagtgata tgctgacaca cattttaaat aataactata acaactatac tattggatta
      841 ttcttatttc tcggattgat tggtgctttt atttttataa aaaaattaac tgctgatgat
      901 ttatttattt tctttcttgg tgtatttgtt cttttttgtt caaccaattt atttccttgg
      961 caacttttta accatacacc agttaagagt ctacaatttg tctggcggtt aaatggtttt
     1021 tcttctttat ttatagccta tacgatgagt atagtgatct attatatttt ttctactaaa
     1081 aataattcat ggaaaataat ggtctttacg tttcttgcac taatattgca tctctcagga
     1141 gtaagcaatt ctatccatgc taacaaagat tcattaacac ttatagcacc tgaagacgct
     1201 gttgctatag ctgaaaatta caatcatact gattatgcaa acaaagagtc aatctatcat
     1261 cctgatatga ttaataatga tcaatattta ctaggtaacc aggaaataaa tccaacaact
     1321 cattttatga gtaacaaact tacaattgaa ttagataatt cgaataataa aaatactgtc
     1381 ttgacgacac ctatttatcg ttataaaggg caagtggctt ctattaatgg caaactagta
     1441 caaacaaaat tatctaagtt tggcacaact gaattaacta ttcctccagg tataaataaa
     1501 gtcgttatta cttatcagta cacaaaacta gcaattgctt caagatattt atctatcgtt
     1561 actttgattt tatttttact ttatcgattt actttttcta aacaaaaaca accgagaaga
     1621 gaagtacaac attctcacta gatttttatt taagaaagac tttttagctg tacacataaa
     1681 caggctaaaa agtctttttt tatatttctt taatttgctt catacattat ttaacatcga
     1741 tatttatact gtgaaacatg cgtttcatcc attatcaaag tatttttatc gataataaac
     1801 ttcatccaaa aacagacctt gtgcagggac ggtttctcct gcatgttgcc gcactttgct
     1861 ttcaaatatt tcatcgattg tttctatcgg catggcacct gtgccgattt ctagtaatgt
     1921 ccccatgata atccgcacca ttttatataa gaatccgtct ccgacaaaag taaaatgaag
     1981 catttctccc tttcgttcga tagataattc atcaatcgta cgcactgtgg atttttttga
     2041 ttttttcaat gcagaaaaac caatgaaatc atgtgtaccc actaattttt cgcaagctgc
     2101 attcagcttg tccatatcca actcttgagg gaaataaaag ctgtggtttc gctcaaatgc
     2161 tgaaggaatc acgttgttcc aaacataata gctgtatttt ttgcccaccg cattatatct
     2221 tgaatggaag cgctcaggca tttcttcgat tcttttgatg acaatatccc gcggaaggta
     2281 acgattcata aagtcgatca tttctgccag tccaatttct gtgtttgttt taaaatttgc
     2341 tacttgtcct cgcgcatggg tgccagcatc tgttcttcct gatccaataa tttctatctc
     2401 ttcatttgtc atctgatgca atactttttc gatttttcct tgaatggttt tatcgtcatc
     2461 tccaagtctt tgccatcctg aatatctttt tccgtcatat tcgattgtca acttgatgtt
     2521 tctcatattt tagtggctcc tctgtcttcc atttggtctt ctctctacct ttttaagatt
     2581 gtagcacaaa atatagctcc tttcttctct aaaacagaag ctatacaaga aaactgtgga
     2641 aatatatatt tcagaaaaac aaaaaacagc caccggatga agtatccgct gactgttcaa
     2701 tttgacggct caagttttac tgtttgagat gcaagatact caaccaggca atgggcacat
     2761 aattaaagaa aaaactgatt ctttggcaaa aacctatttt attcagtaat ggccaagaat
     2821 ttatagaagg aattcttgcc agtccataaa tcgccgcaaa aagcaaactg atcacagaca
     2881 gtccaaggta taagtggctt acgtccattt gcccattttt tcgatagatc aagaaaagaa
     2941 gaactggaaa caataaaaaa ccactgtaac ctaaacctga tccgatatta tggacccatg
     3001 tcgagacatt ccacgaagat tcatgggtat tgatactgaa caaacctgta aagatacaat
     3061 cacctatacc atagaacgcg atggctgctg taactagggt agctaacaca cgagatgtac
     3121 ttacaaatgc agcatacaat gcaggcaatg acaggatgaa aaagatccct gaaaccacag
     3181 accaaataag aaacgctttt cttacaggac tggaaacctc tccaaataca ctgatcacgc
     3241 tcttcatttg atctaattca ggataataca atcctaatac gtacggagtc aaaaaatcac
     3301 tcagcacgcc taataataaa aagaaaaaac caaatttacg taaaaaatcc atgatacgcc
     3361 tccaataaca attacctatg cttttaattg ttattataag cgactttatc ttgatttaac
     3421 agaaacctct atttacacga acatctatat aactcattaa aaactcgaat cattttaaaa
     3481 atactaaata tctaacgttc aacgttcact taatccatct aaacaatctt tcaatttgat
     3541 tttttcccca cttcttttac gcgcgcctag ctctcctatt gcagtaaaca acagatcact
     3601 tgacgaattt aaggcagttt ccattgaatc ctggataaca ccgataataa atccaatacc
     3661 gacgatttgc atcgcgacat cattagagat accaaacaaa ctacatgcta atggaatcaa
     3721 taacagtgaa ccgcccgcaa cacctgaagc tccacaagca gctaaagcag acaaaataca
     3781 taaaaggact ttcaaaacaa aaggtgcttc aatccctaaa gaatgtacag cagtcaacgt
     3841 catcaaggaa atggtaatcg cggcaccgcc catattgatc gtagcaccta acgggatcga
     3901 tatggaataa gactcttttg tcaatttcaa tctttcagcc aacatcatgt ttatcgggat
     3961 atttgcggca gaacttcggg taaagaaagc gggaagtgcg ctttctttca aacaaaagaa
     4021 aatcaatgga tagggatttt ctttaataaa caaaaacgtg aggaatgggt acacaactaa
     4081 tgcgacaaac agcatcgtac cgatcaaaac aagtaaaagc tgcagatact ctgccaatcc
     4141 agctattcca gtcgtagcaa ttgattgata aaccaatcct aaaatcccta tcggagcaaa
     4201 tgcaataatc acttgtacga cttgtgacaa cgctatagat atctgatcca ccaactcttt
     4261 tgtcgattct gaacgtgctc gaaaagctag tcccaacaat acagcccaga ataagacgcc
     4321 aaggtaattc gcatcaataa gagattgaac aggattttgt gtgacattcg tcaacatagt
     4381 agataaaaca ctttccaaac tttgcggtgc tttttcagat actgtttctt gtaaagtaat
     4441 aggaacagta aaaattgaac ttacaataac tgctacaagt gcagaaagca atgttcccac
     4501 taaatataag atcaaaatag gcttcacgaa tgtcttattc ccaattttat gcttggcaat
     4561 tgacgccatc gtcaagaaaa aaaccagaat aggtgcgatt gccttcaagc aactgacaaa
     4621 caacgttccc aatattgaaa taaatgtcca tgtggggaaa aagataccaa acaatattcc
     4681 gactaaaata ccaacaacaa tacgttgaac taacgacata tccaaaaact tctctaacaa
     4741 atgaccaccc tttcacaatt ttaacaatca ttgtatcatt ttatgcaaag aaactaaagg
     4801 tataattttc taaaaattct aacaatacaa acaaccattt cttgctaatt ttgtactttt
     4861 tctgtctaca cgttgttttt tgagatattc taagttagga tgttattaat gggcaactta
     4921 tcaggaaacg ttttcttatg tttttgtcca atttttttgt gaggtgttta ttcttgaaaa
     4981 aaataaagaa ggagagttat ggcttcctga caatctttgt cgcttgtata atgatggttt
     5041 tacagcgtat attgatttca gcagtcacta ttcctacata tgcaacaaac cctggtcgat
     5101 tctacatcta tacattttta caaacttccg ttgttattgg tgcaaatttg atcccacttt
     5161 acatgggtta ccaatatcaa aaaatcgccc gtttaaaagt tttacattat ttaagtcgtt
     5221 ttgcctttat attcctaata gccacactag tttgcaatat ctttttttta cgtacaagct
     5281 ggcagcttga atattcgtga ttattggctt atcctaacac ctatcagtca gaattacttt
     5341 acttatgctg tttcttgtac tttgcttttt tgtagtattc cttatacagt tggattttta
     5401 aataaattat cgaaaacaac aattaaatat ctactattat ttttaacaat tggattagtt
     5461 ggctgctcta ctctatttaa taaagacccg tttgcattac agaatggaca gagtatttta
     5521 tggatttttt atttattttt aatgggttat ggtttgaaag aatggaactg gaaagaaaag
     5581 gtccgctata agcttcctca agtatttatc agcttgattg ttttattcgg tttgattatt
     5641 ttgatgacac aaatttcact catcatcaga ggaaatacag atacagcgct gaggttttct
     5701 aaaccatatt ctctcttcgc aatgtattat actttctctt cgtttttatt tttagaaatg
     5761 ctatctgata aattaggtat aaaaggaaga ggaaattttc ttgctgtatt tttgattgtc
     5821 actcaagtta tatgtaattt ccctttagtt tcttatcgcg tatctacatt tatgaaacgt
     5881 gaatttccaa actcagggaa ggcatggctg ataaatatcg ggcagttttt tatttgctat
     5941 ttattagcag tgtccctcct agttatcttt ttattttata tacaaaaaat ccgtttcatt
     6001 catcgtttca tcgaatactt cgcttttgat tcgattgaac aattaactca gcgttggaaa
     6061 aaaagaattc attggctttt tgttcataaa agttattttt tagttgcagc atttttttac
     6121 tgctttacct tcgtgcagat tttcgtttta gaaccaaaag aaggttggaa agaaactatc
     6181 caggttgcct taaaaatatt tactcagcga caagcaccca tgatcctgac aatcatcatt
     6241 attatgggat ttttcttctt actattgttg atttccaatc gtttttggta cgccttgact
     6301 gctaccttga tcattaactt actgctcact atttcgacag taatcaaaat ggaaatgcgg
     6361 gaagagcctg tctttccttc agatttaaaa atactgaccg gattgtcaga actattatcg
     6421 atggttagcc cagtcttatt aatcgtgggc ggattgatcc ttctcctatt attgattact
     6481 agtattattg tacaacgtag actacaaaaa cagtattcct tgaagattca ttggaaacgt
     6541 cgatttatca gtatcgctgt attattagga tgtttttcgg gtgtcttttt cattaatcac
     6601 aaaaattcgc cttctttctt gctgtttaat ttatttaaag taaacaagac tttctttaac
     6661 cagcaaggcg ccgtaaaaga taacggacca gtcattcaat tcctaaacaa tatcgacatt
     6721 aaaatcatgg aagaacctgc tggctattcg aaagaaaaaa ttaatggaat aatgaaaaag
     6781 tacaacacaa aggcagatga aatcaaccaa acaagaagtg actggttaaa caatcagacg
     6841 atcgttctca atttaagtga aagcttcagt gatcctgcac gtgtccctaa tctgacagta
     6901 cctacagacc ctattcctta catcaaaagc attataacag aaggaacagg aggattgatg
     6961 ttatccgtcg gttatggtgg cggaacagct aacatggaat gggaaggact gacaggcttg
     7021 agtatcagta acctgtctgc ttcgttagtt acaccttata cccagctagt ggagcgtcag
     7081 aaaataagtc caaatattac cgatctattc gatgaaaaaa tagccatcca tccttttaca
     7141 gctgctcttt atcgcagaaa acaagtgttt gataaatttg gttttgacag gttctattat
     7201 ttagacagtc cggataaatt aacttatacg gataaaatag aacaaagccc acgcatatcg
     7261 gatgattcag catacaaaga aaccttgaaa gctttacaaa gcaatacaga aacgtcacaa
     7321 ttcatccaat tatccactat gcaaaaccat atgccttatg atgattacta tagtgaattg
     7381 aactacactg ctgaaggaac tgctgtcatt cctaatagaa gaaaagaatt acaaacttat
     7441 atgcaaggcc ttcactatac cgatacagca gtaaaaaaat tcatccaaga aatcgacaaa
     7501 atcgataaac cgattacctt tgttttttat ggtgaccatt tacccgcaat ctatagtggg
     7561 aatgattcga aaaaatatgg attagaacag caccagacag attatttcat ttataacaac
     7621 aagtatagtc gtgaacaagc agctacgtta aataaagaag ttgttgctcc taataacttt
     7681 ggtgctatgg ctcttgagca tgcgaatatc aaagtaactc cttactatgc gttgttgaca
     7741 gaagtatcta atcatctacc tgcattaaca attgatccta cagaaagctt gtctaatcgt
     7801 tttaatggaa aacaggtctt tgttacagag gatagtcaaa ttatccaaga aaaaaattta
     7861 actgagaaac aaaaagaaat tctagaagat tatcgtctga tacaatatga tcttgttgca
     7921 ggtaaccagt acagtgcaac atgggcgaca caaaaaat
//
LOCUS       DAYYKN010000124         7854 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000124 DAYYKN010000000
VERSION     DAYYKN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.126"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(110..1120)
                     /gene="trpS"
                     /locus_tag="ACHBB7_002344"
     CDS             complement(110..1120)
                     /gene="trpS"
                     /locus_tag="ACHBB7_002344"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM9210253.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            1374..2111
                     /locus_tag="ACHBB7_002345"
     CDS             1374..2111
                     /locus_tag="ACHBB7_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9210254.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            2125..2940
                     /locus_tag="ACHBB7_002346"
     CDS             2125..2940
                     /locus_tag="ACHBB7_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9210255.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            2953..3600
                     /locus_tag="ACHBB7_002347"
     CDS             2953..3600
                     /locus_tag="ACHBB7_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9210256.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            3616..4272
                     /locus_tag="ACHBB7_002348"
     CDS             3616..4272
                     /locus_tag="ACHBB7_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9210257.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            4434..5255
                     /gene="racE"
                     /locus_tag="ACHBB7_002349"
     CDS             4434..5255
                     /gene="racE"
                     /locus_tag="ACHBB7_002349"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM9210258.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            5258..6613
                     /gene="rph"
                     /locus_tag="ACHBB7_002350"
     CDS             5258..6613
                     /gene="rph"
                     /locus_tag="ACHBB7_002350"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM9210259.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            6614..7132
                     /locus_tag="ACHBB7_002351"
     CDS             6614..7132
                     /locus_tag="ACHBB7_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9210260.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            7239..7739
                     /gene="cbpB"
                     /locus_tag="ACHBB7_002352"
     CDS             7239..7739
                     /gene="cbpB"
                     /locus_tag="ACHBB7_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM9210261.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2540 a         1359 c         1682 g         2273 t
ORIGIN      
        1 gatgtgacac aacttcgtca catctctttt tttgatttta aaaaaggaat agcacagaga
       61 cgagataaaa aaacaggcct ttacagcctg cttttttaga gtaaatgaat taatggaagt
      121 aattgattcc cattgctgat tttacttcag aaagtgtttg tgcagctact ttagcagctt
      181 cttcactacc tttacgtaag atttccataa cagctgaagg atctttttcc aattcttctc
      241 ggcgcgcacg gatcggtgca aattcagctt ccaatacatc aatgaggtat cgtttgatct
      301 ttacatcacc aagtccgccg cggcggtaat gagctttcat ttcttcaatt gcttctttat
      361 ctgttccgaa tacatccaag taggtgaaga ccatattccc ttctacttgt ccaggatctt
      421 gtacatggat gtggtttgga tcagtataca tactcatgac ttttttctgt aagacatcgg
      481 caggatcgga gatataaata ccgtttccta acgatttact cattttgcca tttccatcga
      541 ttccaggtaa tctacccatt ccttttggtg ggaatacgcc ttttggttcc accaagactt
      601 caccatatgt atgattgaag ctttgtacga tttcctgtgt ctgctctagc attggttttt
      661 gatcctcacc aacaggtaca agattcgctt tgaatgcagt gatatctgct gcttgagaaa
      721 caggatagat aaagaatcct gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg
      781 ttttgacagt cggattgcgc cgaacgcgtc ctacgctgac taagttcaaa taatacatgg
      841 ttaattcagc taattccgga atttgtgatt ggataaataa agtagatttt gcaggatcta
      901 atcccacggc taaatagtct aaagcaactt gcaagacatt tgatgaaacc ttctcaggat
      961 ttttagcatt atctgtcagt gcttgcatgt cagcaatcat gacaaacagt tggttgtctt
     1021 catctgcctg catagccact cgggttttta gagaaccgac gtaatgccct aaatgcagct
     1081 tcccagttgg tcgatctccc gttaaaataa tatttttcaa aaaagacacc tctttacgat
     1141 ttgtaagtct gagcacttaa tatcttaccg tttttcctga aaagaaacaa cagttcctaa
     1201 ggaaaaaaca aggaaagagg ataaaataac taatagatta gaaatggaag aaatatttat
     1261 gggagaataa acgttctaag aaactttaca aattactaaa ctatcagata taatgtattt
     1321 tattatacat ctttaaaaga aatcttacta aataataatg ggagtgtaat tctatggcaa
     1381 tgattaaatt tgatcacgtg gataaatatt atggcaagtt ccacgcatta aaaaatatca
     1441 atctcgaatt cgagaaagga gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga
     1501 gcacgatgct tcgctgtatc aatggattag agacgatctc ttcaggtaag ctgctgatta
     1561 atgatactga cttgcatgat aaaaagacaa aactgacaga agtccgaaaa aacatcggca
     1621 tggttttcca acatttcaat ttgtacccaa ataaaactgt tctggaaaac atcaaattag
     1681 caccaatcaa agtgttgaag caagaccagg caactgcagt caaaaacgcg gagaaatttt
     1741 tgaaaactgt caatatgtta gataagaaag attcttatcc ttccatgtta tccgggggac
     1801 aacagcaacg ggtggcaatc gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg
     1861 atgaacctac ttctgctttg gatccggaaa tgattggtga tgtattagac gttatgaaaa
     1921 aattggctca tgacggtatg tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag
     1981 aagtggcgga tcgtgttatc ttcatggctg atggagaagt attggaagac agcagaaatg
     2041 tgagagactt tttcgaaaat ccaaatgaag tccgagcaca gcaatttatc agtaaagtta
     2101 tcaatcattg ataggaggaa aaagatgcgc aggttaaaaa aaatacgcct gattttcctt
     2161 ttaggcattg cttttcttgc actagccgga tgtaaaggaa atgggttggc aaatgaagat
     2221 attttagagc gaagcaaatc taccaatgaa atcatctggg gtgtcaagta tgatactcga
     2281 ttatttggaa tgatggatat tgagagcaga actgtacaag gatttgatgt agatattgca
     2341 aaagcaatca ctaaaaagat ccttggtgac aatggaaaaa cagaatttgt tgaagtcact
     2401 tctaaaactc ggattccatt attgaaaaac ggaaatatcg atgcaattat tgcaacgatg
     2461 acgatcacag atgaacgaaa aaaacaggtt gacttttcag atgtctattt cgatgctggc
     2521 caagctttgt tggtaaagaa aggaagccaa atcaaaagtg tagatgattt gaatgcttct
     2581 acaacggttc tagcagtcaa aggttctaca tcggctgcga atatccgaca gcatgcacca
     2641 gatgcaaaaa ttttagaatt agaaaactat gcagaagcat ttactgcttt acaatctggt
     2701 cagggagacg cgatgactac ggataatgcg attttgttag ggattgcaga tgaaaatcca
     2761 gagtatgagt tagttggtgg aacgtttact aatgagcctt acgggattgc gatcaacaaa
     2821 gggcaagaaa acttcttgaa agctgttaac caagcgctag aggaaatgca tgcagatggc
     2881 acatacgata aaatctatca aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa
     2941 gggggagagg caatgagcga attgatacaa acttatggac caatctttgt cgacgggttc
     3001 aagatcacat tgttttcgag tgtcttagca cttgtgttta gtttgattat cggaacgtta
     3061 atggcaatct tccaattatc aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta
     3121 gaatttttta gaaacattcc attattaatc atcgtgatgt ttttttatgt tgtcgtaccg
     3181 atttattggt ttaatctaga tggattccaa gcaggtacga ttggattaac gatctataca
     3241 tctgctttta ttgctgaaac agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca
     3301 gaagcaggac tatcttctgg ttttacctat tcagaaatca tgcgttatat tgttttaccg
     3361 caagcattca aaatcgttat cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac
     3421 tcatctattt tagcaatcgt agctggttta gatttgatgt atcaaggaga cttgatcgct
     3481 agtgaaacat tcaatacctt cgatacgtat atcatcgttg ggttatttta cttagttatc
     3541 acgctaccgt tatcttactt aatggtttat cttgagaaaa aatgggcggt tcgttcatag
     3601 aaggagggga aatacatgga ttttagcggc gctttttcat ggatgaatat ccgtttttta
     3661 ttggaaggat taaaggttac tgttgaagtt tcattgtttt cgatcatttt tagtttcttg
     3721 atcggtggtc tgacaggtgt gattcgtttt gcgaatatcc ctttcttttc caaaatcctt
     3781 ggtgtagtga ttgatattat acggaatctt ccattattat tgatcatttt cttcacttac
     3841 tttgccttac ctcaaatcgg cattcagatg aacatcttct ggtccgctgt cgcagcatta
     3901 acgatttttg agtcagctat gttgtcggaa attttccgag ctggactcaa tgctgttcca
     3961 aaaggacaga tggaagcagg attatcaacg ggactgactt atgtagaatc gatgcgaacg
     4021 atcgtgttac cgcaagcatt taaatcaatg atcccggcaa ttgtgagcca attgatttca
     4081 ctgatcaaag atacttcatt agcagtaatc atttctttac ctgaactttc tcatcaggca
     4141 cgtattattt atggacagaa caccaattat gtgcttccga tgttcatcat catgacactt
     4201 atgtacttcg tggtatgtta cgcgctttct ctgctttccg gttacttgga aaagagacgg
     4261 tacagttatt aatagatgat tcttttagcg gttgtggcaa actaaagtca caaccgcttt
     4321 tcctctttcc attcgaaaaa aagagatttc gattgtttta agcaagtaag agcacttgaa
     4381 aattcacgct aaaaagtaca caatagggag gatagaattt attgaaaaaa aggatgatac
     4441 gattgacaga taatcgccct atcggattta ttgattcagg tgtcggcggc ttgactgtag
     4501 taaaagaagc cctgaaacaa ttaccgaatg aaaatatttt atttgtagga gacacagcac
     4561 gctgcccata tggccctaga cccgcggaac aggtaatcca gtatacttgg gaaatgacgg
     4621 attatctggt ggagcaagga atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg
     4681 tggctttaga agaaatcaaa gctgctcttt ctattccagt catcggtgtg atccttcccg
     4741 gtactagagc ggcagtaaaa aaaacacaaa ataaacaagt tggcattatc ggtacgattg
     4801 gtacggtaaa aagtcaagct tatgaaaaag cactgaaaga gaaagtacca gaattgactg
     4861 tgacaagtct tgcttgtccc aaatttgttt cagttgtcga aagtaatgaa taccattcat
     4921 cggtggcgaa aaaaattgtg gcagaaacat tagctccttt aaccactaaa aaaatcgata
     4981 cattgatttt gggatgcacc cattatccat tattacgccc catcattcaa aatgtaatgg
     5041 gagaaaatgt tcaactgatc gattctggag cagaaacagt aggtgaagta tctatgctgt
     5101 tagattattt caatttgagc aattcaccgc aaaatggtcg gacattatgc cagttttata
     5161 caactggctc tgccaaactt ttcgaggaaa tagctgaaga ctggcttgga atcggacact
     5221 taaatgtaga acatatcgaa ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc
     5281 ccaagagctt cgcaaactgg agcttcaaac aaatgttttc aaatatccag aaggatcggt
     5341 agtgatccgt tttggtgata caacagttat ttgttctgca acaatagaaa attctgttcc
     5401 cccttttttg agagaaacag gaacaggatg ggtgacagca gaatatagta tgctgcctcg
     5461 agcaacgagt acgagaaatc gtagagaaag cagcaaaggg aaactttctg gtagaacaat
     5521 ggaaatccag cgtcttattg gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg
     5581 agaacgtagt atcatcgtag actgcgatgt gatccaagcg gatggcggta ctagaacagc
     5641 aagtatcact ggggcatttg tggcattgaa attagccatt gaaaaacttt taagagaaaa
     5701 agaattgtca gaagatccaa taaaagaaca tcttgctgca gttagcgtag gaatcttacc
     5761 agatgggact tgcgtcacag atttggatta ccaagaagat tccgcagcac ttgtagacat
     5821 gaatcttgta atgacagaat ctggaaaatt cgtggagatt caaggaactg gagaagaagc
     5881 aacgtttgat ggtgaacagt tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga
     5941 tttgatcaaa gagcaaaaac atgctttgct aacagagttt gctcaaaatg atgagcggat
     6001 agaagaaaca aaaacaatca tcattgcgac aagaaatcca ggaaaagcag aagagtttcg
     6061 aaatatgttc aaagaagcgg gatatcatgt aaaaacatta cttgactatc ctgaattgcc
     6121 agatgtggaa gaaactggca gcacatttga agaaaatgcc cgattaaaag cagaaacgat
     6181 tgcacaattg cttgatcagc cagtcttagc agatgattca ggtttgaaag tggatgcttt
     6241 aggcggtatg ccaggaatct attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg
     6301 gaataacgcc aaattattat atgaattgac agatgtgccg gatgaaaaaa gaacagcaca
     6361 attccattgt acgttagtat tcgcggcacc caaaaaagac agccttgtag tagaagcgga
     6421 atggcccggt cgagtagccc gtataccatc tggcgaaaat ggctttgggt atgatccttt
     6481 atttatccca gaaggtaaga aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa
     6541 gatcagtcat cgggctcaag caatgaaaaa attgagtgcc gagtggaaac aatggctgga
     6601 aggagagaga tagatgcggt atttagtagt aagtgataat catggagaca gagatatcct
     6661 cgttgactta gtagatcgtt atcgttctga agtggatatc tttttccatt gtggtgattc
     6721 ggaattagaa gcttctgacc cattatggga tacattccaa gtggttcaag gtaattgtga
     6781 ttatggacca ggattcgaac agaaaaaagt gatccaaact ggtcaggata cagtattcat
     6841 gactcacggt catttatcga atgtccgatt tggtttgaca cagttagcaa tcgaagctcg
     6901 agctgcaact gccgatatgg tgttttttgg tcatacccat caaataggct gtgagatgga
     6961 tcagaaaatc ctgtttttga atcctggtag catttctcag cctagaggcc cgatccaaat
     7021 cccatcatat gctcttatcg aaagtttaga tgaccaaatt tctgttcaat actataaccg
     7081 tgcccatcag gcagttgaag agttggcctt ttctttttcg agagagcgct gaataaaaaa
     7141 actctaattt ttaataaaaa taagctttat tttatgaatt ttgcatccgg tttcggtaaa
     7201 atagtagtgt tgaatgaaat agaaatggag gccatcagat gattggacct atagtaagag
     7261 aaatgctgct ggaaaatcag gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta
     7321 tgtatcatca tccattgtcc catggtttac ttgtgttatc gaaggtcggt tattccaaaa
     7381 ttcctgtatt aggaaaagat gatcactttg taggactagt aagtttatca gatgtggtca
     7441 ataaaatgat tgacttgcaa acaatcagca tggacccgtt agaaggacta acggttgctg
     7501 atgtgacaga aacagatgtt ccgacaattg atgaaaattg ggaactagaa gaagtactcc
     7561 atttgttagt cgattcgcct tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa
     7621 tcattacaag aaaagagctg cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc
     7681 gtaatatatt gtctccgaaa atcgaagaat taaaagaaca aatcgatatc gtgagctaaa
     7741 gaacgagaaa ctaaaagata atcaaaaaat aagtagaaaa aacagcgtac cagttttgac
     7801 tttatcttga aaaagaggct gggacaaaag cgtttaactc caagaaataa gaag
//
LOCUS       DAYYKN010000125         7837 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000125 DAYYKN010000000
VERSION     DAYYKN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.127"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..786
                     /locus_tag="ACHBB7_002353"
                     /pseudo
     CDS             278..786
                     /locus_tag="ACHBB7_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            879..2041
                     /locus_tag="ACHBB7_002354"
     CDS             join(879..1155,1155..2041)
                     /locus_tag="ACHBB7_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9210262.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2321..3301
                     /locus_tag="ACHBB7_002355"
     CDS             2321..3301
                     /locus_tag="ACHBB7_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9210263.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3423..4841
                     /locus_tag="ACHBB7_002356"
     CDS             3423..4841
                     /locus_tag="ACHBB7_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM9210264.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4912..6381
                     /locus_tag="ACHBB7_002357"
     CDS             4912..6381
                     /locus_tag="ACHBB7_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9210265.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6391..6858
                     /locus_tag="ACHBB7_002358"
     CDS             6391..6858
                     /locus_tag="ACHBB7_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9210266.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6894..7766
                     /gene="scrK"
                     /locus_tag="ACHBB7_002359"
     CDS             6894..7766
                     /gene="scrK"
                     /locus_tag="ACHBB7_002359"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9210267.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
BASE COUNT         2675 a         1180 c         1600 g         2382 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaggaaata agaaaactaa acttgtatcg
      601 ttaacaaaat atggtcgaaa ggtgtacgaa tcacataatt atttacatga agagctttat
      661 agctcaataa atgaagcgtt agaatcattc tcaccagacc aaatttcaac cattaaacag
      721 attctagaaa aagtatctta tacaatcaat gaatatcaag taagtctgac aagaaaggaa
      781 gattgacatg aaaaaaatga tttttgaatc ggctacacta gactagacag aaaaaataag
      841 gtgtgtaaaa taaatgaaaa cacactggag gaatttttat gtcaagacgt caacgtagaa
      901 cctattcaaa agaattcaaa caacaagtcg tcgatcttta tctcgcagga aagccccgag
      961 ctgaaatcat tcgagattat gagctgacta cctcggcctt tgataagtgg gtcaagcaag
     1021 cgcaaactac tggctcgttt caggaaaaag ataacttgat ccctgaacaa gtagaactaa
     1081 ttgcattacg aaagaaaaat aaacagcttg aaatggagaa tgacatttta aagcaagcgg
     1141 cgctgatatt gggacgaaaa gacaagtaat tgataccaat aagcacaaat actcaatatc
     1201 agcgatgtgt aaagtgttaa acatctcttg ccaaacctat tattatcaag ccaaaccagt
     1261 tgaagatgaa tcagaactgg aagaactcgt tcaagaagaa tttattcgca atcgaaaagc
     1321 ttatggtacc aggaagctga agcagtgctt ggccaatcta ggtcttcaag tgagtcaacg
     1381 cagaatcggc cgtatgatga aacagcgtgg cttgcgttca acatacacat tggctcattt
     1441 caaattccat tcttctactt gtaacgaagc caagaccgcc aatattctag acggaacctt
     1501 cctacaggaa aagtcattag aagcgattgt aaccgacctt acctatgttc gtgttgggaa
     1561 gaaatggcag tatatctgcc tgattcttga tttgtttaat cgagaaatca tcggctattc
     1621 atgcggggag aagaaagatg cgacgttagt caaggaagct tttgcaaaga ttccgtactc
     1681 gttgaataat gtacaacttt ttcacacgga tcgtggaaaa gaattcgata accagacgat
     1741 tgacgaaatt ttatgggcct ttaatatcac gcgttcctta agcaaaaaag gctgccccta
     1801 tgataacgcc gttgcagaat ccacctataa atctgtcaaa gtagaattcg ttcatcaata
     1861 ccgctttgag acattggcta atttgcgatt agagctattt gattacgtcc attggtggaa
     1921 taccatccgg ctgcacggca cgttaggcta tgagacaccg atcagttttc gacagcagag
     1981 attggcgcag cgaatccttg ataatgagct cggatgtgat accggtggcg aggcacctta
     2041 attatttgaa tgagcttctg ccttagagaa tcagattcga ggaggctcgt tgtcaagggc
     2101 agtcggaaca ccagcagcaa gcttacgcaa taccttataa tttttgtcaa aaaaagtgtt
     2161 gccattccac atatatcttt aaacagctat tgccagataa aaataaatga tattatatat
     2221 ataaatactt atccgaaaag gggaatgaga gagtcaaggt tcttggaatg tcgaatagtc
     2281 gagtaggtag attttctatc tacaaccttg ttgtttaaaa atgaaaaaaa ttggaattag
     2341 agaagttgcg aaagaggcaa atgtttctcc tacgactgtg tcgagggtat tgaatgatcg
     2401 tggatatttg agtgaggaaa caaaaagaaa agtttttgat gccatgaaaa aattagaata
     2461 ctatcctaat gaactggcga gagcactttt taaaaacgaa acatataccg taggtttgat
     2521 ttttcctaca attactaatc catttcacgc tgagttaatt caagatatcg agttcgagct
     2581 ttccagcagg ggatataaag tgttgctgtg caatagctta aacaatccaa caaaagaaaa
     2641 agagtacttg acgatgttga gaaagaatca ggttgatgga attatagtag gaacgcacaa
     2701 taaagatatt gcggattatg atattccagg gctacctatt gtagcgattg atagaagtct
     2761 tggtgaaaat acagttacag tatcttgtga taattacagg ggtggggaat tagctactca
     2821 actacttgta aatcatggct gtcagaatat actgtgtatt cggggagata gtaagataaa
     2881 gctacccgcg aatagtagga ctttagcata taaagatgtt ttgaataaat tcatgctgga
     2941 accgttagtc ctagaagtac catttgtaaa agaaatcaat gaaaaaaatg aaataattaa
     3001 gacatatctg gaaactcatc caaatattga cggggttttt gcaggagatg atttattggc
     3061 ttctttggcg attaacaact taggaagttc gggcaaaaga gttccaaaag aggttaaagt
     3121 gattggattt gatggagctc aacaaacatt gatttataat ccagaattat caactattca
     3181 acagcctata gatcaaatat ctaaagtagc agtaaaaaaa ttattgaata tgattaaggg
     3241 cgaaaaggag acggatgaac tttcacttcc agtaaagctc ataaaaaaaa atacagcata
     3301 agttaaatcg gcgaaaaatt ccttcgtcga tttaaaaata tttttatgaa acgggttgac
     3361 atatgaaacg ggttgacata tattagttgt gaagataaaa accttttcac tggaggaaga
     3421 aaatggagaa attattaatt aaaagcaata atcatcaact ttactaccaa cctaaggatg
     3481 tttgggtcgg agatgttatg ccttacggga aagaaggaaa attctttatt tatcatcaac
     3541 gtgatactag aaatccagtt ccttttggag aaccgtttgg ctgggcttta gcaactacga
     3601 aagattttgt tcattttgaa gactttgggg aatctctttt aagaggggat gacaatgaga
     3661 gagaccagtt tatctacgct ggttctgtat ttgaggcaaa tggaaaggtt cacgcatttt
     3721 atacagggta taatcgtgaa ttcttagcgc agggaaagac atctcaagtg ttgcttcatg
     3781 ctgagagtga tgattttgtt cattgggaaa aatcagaaac agcattggag ttagaaccac
     3841 aagaagggta tgatattcga aactggagag atccatttgt cgtttgggat gatgaaaaag
     3901 aagaatattt gctaatttta ggggcgcgaa agggtcagga taaacatcta caaaccggac
     3961 gtttagtgga atttacatca aaagatttaa ctaattggga attcaaaggt gatttttggg
     4021 ctccgggatt gtataccatg tttgaaatgc cagaactttt caagattggt gactggtggt
     4081 atcttgtcta ttcagaatac tcagatggaa ataaaatact ctatcgaatg agtcaaagtt
     4141 tggaaggtcc ttggattaag cctaaagatg aagcttttga tggacgggct tattatgcag
     4201 gtagaacagc ttatgatgga aaacgtcgag tcctatttgg atgggtacca acaaaagatc
     4261 aagaaaatga taaaaacaat tatttatggg gaggtacttt ggttcctcat gaagtctatc
     4321 aaaaagagga tttttcactt ggggtaaaac ccatagacga agtgtggaat gcattcataa
     4381 atttacatga tattgccgat gtagagatag aaacagcaga cacaagagaa gaagtaacgc
     4441 ttgtcaaaaa tagtggaact ttgtttaatt tagaaacaac aatttcattt gatgagaatc
     4501 ttagtgactt ttcactgaga ttatataaaa accttgagac agatgaatct tacgaatatc
     4561 gcttcaattt agatgagggg cgtcttacat ttgataaaaa cccttgttac ccttggtttc
     4621 aatatatgga taaaggactt attagaccta tatctttaaa gcctaaccaa tcttataaac
     4681 taaaagtgat tgttgatgac aatataatga cactttatat tgatggaatc gctcttaacg
     4741 ctcgtgtaca tggttgtttt ggtcaagcaa ttactattac agcaacaaat ggatcaatga
     4801 ctgcaataaa taccaagttt tcaaatgaaa taaagaaata ataaacgtca atcatgtggc
     4861 aggtctgaaa atcaaacatg acctgtcaca tttaaaaggg aggataaatg aatggactac
     4921 aaaaaaattg ccgaagatgt attggaaaac gttggaggaa aagagaatat ctcaagcgct
     4981 acacactgca ttactcgttt acgtttaatt cttaaagaca aaagcaaagc gaatatggaa
     5041 atgcttgagg aaatagaagg tgcaaaaggt gttcttttca atagtggaca gttacaagta
     5101 attttcggaa caggtataat tgaaaaggtt tacaaagagt tcgttgaatt gacaggagct
     5161 aaggaagtat ccgtttctga aatgaaagac gaaggagtca ataattcagg aaatagactt
     5221 caacgagctt ttaaagtttt ctcggatatc tttattccga tcattccagc ttttattggg
     5281 gcagcaatgg ttttaggaat tcgttcgttg attaccactc ctggaatgtt tggaatggat
     5341 ggctcattcg ctgatcacaa tgtattccta aatgatttat ccaaatttct gggtattatt
     5401 gcaaccacat ttgattattt accagtttta gttatgtatt ctgcaacaaa acgttttggc
     5461 ggaaacccta ttttaggatt acttgtagga tttgttatga ttcatccagg actagccaat
     5521 cggaatgatt tcgtcatggg aactataaag cctgattatt ggcatttatt tggattaagc
     5581 attccagcag ttgcgtttca agggggtgtt tttccagcaa ttttgacttc ttggttttta
     5641 gcaaaagttg agaaaattac aaataaatat tctcctcaag tacttgctta cattttagtc
     5701 ccaactgtga caattgttct tgccaacttt gcactatttt tagtgtttgg tccattgggg
     5761 aattttatcg gtaatgtttt aggtggaatc attgatttcc tatatatgaa gatgggtgcc
     5821 tttggtgctt ttgtttttgc aggcgcgtta caaccattag ttgtaacagg aacacaacat
     5881 gctattcaag gtattgaggc aaacttagtc gcaacaactg gattcaatta tatccaacct
     5941 atttggtcag tttcaattat tgcccaaggt ggcggttgta ttggtatgta tttactcttt
     6001 aagaaaaaat ctaaagatag agatattgcg atttcaagct ttattccaac actggtaggt
     6061 attactgaac cggctatctt tgctgttaat ttgaaatatt caatcgttcc ttttgtttgc
     6121 tcgtttattg gtgctggttt tggtggagca tttatgaaaa tttttgatgt aaaagccatt
     6181 ggacaaggat tgactgtatt gccaggtctg actattgcta atccagttct tccttatata
     6241 tttggtaatc tcatagcatt cattttacca atcattttta ttctcgctta tgataaatat
     6301 aaagtgttta tccctgaagc taaacaccgt aaatttgcag aaaaaactga ctcaaaaaaa
     6361 ttgccagttc aagtaaagtg agatgagagc atgttaaatt tttttagaaa aaatgaaata
     6421 agcgttgtag cacctgcaac cggtgttttt gaaaaactgg agaaattaag tgatccagtc
     6481 ttttcaaaag gaatgatggg acaaggattt gcaattgatg caaaaaacga aacgattgta
     6541 tctccaatcg atggttttgt ttcaagtatt tttcctacaa aacatgcctt aggaataaaa
     6601 acaaaatcag gaattgagat acttgttcat gtaggaatag atacagttga gctaaatggt
     6661 gagggatttg atattaaagt tcaagaagga caaaaagtta aggctgggga tattcttatg
     6721 accattaatt ttgtagtttt tgaaaagaat cacaagacta aagatgtcat tgttgttttt
     6781 ccagactacc aagacgaaat tttcattccg aaacttgaag aagtaaaaca aggacaagaa
     6841 ttgtttgttc ttggctaatg ataagtaatt aataatagaa tcgaggaaaa tgtatggttt
     6901 ttggggctat tgaagctggt ggaacaaaat ttgtttgtgc cattggagat aaagatttga
     6961 acatatttga aaaagtcagc tttcccacga ctacacccaa agaaacaatg aatttagttt
     7021 tagatttttt taatcatcat aaagatgatt tagaaggtat cggagtggga tcgttcggtc
     7081 ctattgatat tcattgtgaa tctaaaacgt atggcttcat taaatcaacg ccaaaacttg
     7141 cttggcagga ttttgatttt attggaagtt taaaaaagca acttaatatt ccaactgctt
     7201 ggacgactga tgtaaatgcc tcttgctatg gtgaatatgt ttctggaaat ggcagaggcc
     7261 tttcaagtgt tgtttactat accgtaggaa cgggcatagg aggtggcgct atccaaaacg
     7321 gaaacttcat tgaaggtttt agtcatccag aaatgggaca tatgcttgta aaacgacaca
     7381 ataaagacaa cttttctggg acatgtccat tccacggaga ctgtttagag ggcttagctg
     7441 ctggtccggc aatcgaaaga agatcaggga aaaagggaca agaagtgact gaaaatgacg
     7501 ttacttggga aatagaagct tattacattg ctcagtgtgc atataataca acattgatgt
     7561 tatcacctga tgtcattata tttggtggcg gagtaatgaa acaaaaacac ttaaaaaaga
     7621 aagtacaaga taagtttatt gagttgatca acggatatgt cgctattccc gatgttgaca
     7681 attatattat gacaccaaaa ttgttagata attctggtat tgtaggttgt ttagctttag
     7741 caagagacgt taaagaaaaa tcttaatttg gaaaaaagat cgtctcgact atttgtttgg
     7801 ttgatgcgat ctttttttca attagtaatt gaaggga
//
LOCUS       DAYYKN010000126         7797 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000126 DAYYKN010000000
VERSION     DAYYKN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.128"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACHBB7_002360"
     CDS             complement(105..629)
                     /locus_tag="ACHBB7_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM9210268.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHBB7_002361"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHBB7_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM9210269.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHBB7_002362"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHBB7_002362"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM9210270.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACHBB7_002363"
     CDS             complement(2351..3031)
                     /locus_tag="ACHBB7_002363"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM9210271.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACHBB7_002364"
     CDS             3157..4026
                     /locus_tag="ACHBB7_002364"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9210272.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACHBB7_002365"
     CDS             4052..4567
                     /locus_tag="ACHBB7_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM9210273.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACHBB7_002366"
     CDS             complement(4614..5177)
                     /locus_tag="ACHBB7_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9210274.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACHBB7_002367"
     CDS             complement(5232..5411)
                     /locus_tag="ACHBB7_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210275.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACHBB7_002368"
     CDS             complement(5488..5997)
                     /locus_tag="ACHBB7_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210276.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACHBB7_002369"
     CDS             complement(6093..6473)
                     /locus_tag="ACHBB7_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9210277.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHBB7_002370"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHBB7_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM9210278.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2232 a         1614 c         1382 g         2569 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaa
//
LOCUS       DAYYKN010000127         7783 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000127 DAYYKN010000000
VERSION     DAYYKN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.129"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(575..1921)
                     /gene="gor"
                     /locus_tag="ACHBB7_002371"
     CDS             complement(575..1921)
                     /gene="gor"
                     /locus_tag="ACHBB7_002371"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM9210279.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2055..3308)
                     /locus_tag="ACHBB7_002372"
     CDS             complement(2055..3308)
                     /locus_tag="ACHBB7_002372"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM9210280.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3314..4237)
                     /locus_tag="ACHBB7_002373"
     CDS             complement(3314..4237)
                     /locus_tag="ACHBB7_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM9210281.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4458..6950)
                     /locus_tag="ACHBB7_002374"
     CDS             complement(4458..6950)
                     /locus_tag="ACHBB7_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM9210282.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6957..7424)
                     /locus_tag="ACHBB7_002375"
     CDS             complement(6957..7424)
                     /locus_tag="ACHBB7_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM9210283.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7560..7633)
                     /locus_tag="ACHBB7_002376"
     tRNA            complement(7560..7633)
                     /locus_tag="ACHBB7_002376"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7597..7599),aa:Arg,seq:acg)
     gene            complement(7691..7764)
                     /locus_tag="ACHBB7_002377"
     tRNA            complement(7691..7764)
                     /locus_tag="ACHBB7_002377"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7728..7730),aa:Pro,seq:tgg)
BASE COUNT         2168 a         1695 c         1252 g         2668 t
ORIGIN      
        1 aacaccgttt atcagaggtt gtaaaatcag tgttttgtcc tgagtattag agaaacaaat
       61 ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgaagga cagctgattg
      121 aacactattt gttcggaaaa agtgactaac tatgacacaa tttttttcac agtccctttt
      181 ttcacttgat ttgattcgta ccagcgaact tgacgagaac tttcttggtt tggtaactat
      241 atggtattct tttaacatcc tcgtttgctt cttctaattt atttatacaa agtcacactt
      301 ttttaaacat ctgttcttcg atacatttct atttctttta aacatcgata ccaatatttt
      361 gaacttaaga gtgcatacca aatggattca tttactcttc gtcagttacg aagataatca
      421 aaatctccaa cttccctact tatttcgttt tgagaattat cgaagcaatc ttacataaat
      481 aactaaaaaa caaaaaacca gaaaatcagt atactgattt tctggtttta catggtttgt
      541 tcaataaaaa gctgttggaa ctgttaatat taaattatcg catcgttacg aattcttccg
      601 ctcctgttgg atggatggct actgtattgt caaagtcttc tttagttgca cccatcttga
      661 tagccacggc aaaaccttgc agcatctcat cgacaccaac accaatagca tgtaagccga
      721 cgattctttc ttcttttccg acacagatca atttcatttc gcatttttgg cgataatcat
      781 ttaatgcaaa gtacattggg gtaaagcgtg aacgataaac tttcaattca ttttcgccat
      841 atttttccaa tgcttcttct tctgtcaaac caattgttgc aattggtgga tgcgtaaaga
      901 cgactgtcgg aacaagttta tagtcaagat aagaatcttt ctttccatta aataaacgtt
      961 ctgataaacg tctaccagct gcgattgcaa caggagtcag atctaattta ccgatcacat
     1021 caccaacagc aaaaatatgt tcatccgttg tattttggta tttatctact ttgataaacc
     1081 cttttttgtc caattccaca tttgtatttt ccagacctaa tttttcggta ttcggtacac
     1141 gtccaccggc aaaaagtaca caatcaccag ttaaggtttc accattttca aatagcaccg
     1201 tatattcgtc attgttttta gtaatttctt tagcaatatg atgtgcgtaa gtttttattc
     1261 cagcttcttg gtacatttgg atcaaattgt ccgaaagcat tttatcaaac gttctaagtg
     1321 gtcgttcttt tcgatatgcc cacatgactt cactacctaa accacgaaat acacctgaaa
     1381 gttcagccgc aatgtatcca gcacctaaaa cgattgctct cttaggtaac tctgttaatt
     1441 caaaaaatcc atttgaatca attgcgtatt ctccaccagg aatggtcatt ttacttgcac
     1501 ggcctccagt agcgatcaag attttaggag cacgataggt ttcaccgttt acttcgatcg
     1561 tttgactatc gataaaagta gcgtaacctt ttatagcctc gacatgattg ctatctaatc
     1621 ctctttgata agcaccgtgt agaaaatcaa tatatttttc tcgtctttct actagaccgg
     1681 cgaaatcaac attttttaaa tctgcttgga taccgtaact tgaggcatct cgttcaattg
     1741 tttccaaaat cgtacttgct tgccacataa cttttttagg tacacaaccg acgttgacac
     1801 atgttccgcc caattcattc gcttcaatca gcaaaacttt tgcgccatgc atccctgctc
     1861 tgtttgctga agcaataccg ccagagccgc caccaattac gatgtaatca tatatttgca
     1921 ttttgttcct ccttattgaa gagatcaatc aaaaccttta tttaacgatc ctttttctac
     1981 agaaaacaag tgtaacactt ttcttgaatt ttgtatgcga agatgcttaa aacagggata
     2041 tatcagcttt ttccttattt acgttttcgg atcatatctc caggtttcac cggctgtgcg
     2101 actggcatag ttaaaatcat cattggattt ggtgctcggt caatggattc gccttcttca
     2161 ttatgcataa cttcaacggt ttgactgaaa tgactgaatc ctggaccata aaattcgatt
     2221 tcatctccta cagaaaacaa gtttctctga cgtatcgtag caatctgtgt tgcctcatca
     2281 taagataata cttcaccgac aaatttatat actgggattt tacgacgttc accgaataat
     2341 tgttcgtttt cagttggtgt attgtagtaa aaacctgtag ctaattctcg ttgtgctact
     2401 ttccataact cgtcgatcca ttcttgcttg cagacatagt tttcaggatc ttccatataa
     2461 ctgtctacag ccgctttata gacatttgac accgtagata cataatggat cgatttcatt
     2521 cttccttcga tcttgaagct gtctacgccg ttttcaatca attctggaat atgttcgatc
     2581 attgacatat ccactgcact catcgagaat tcttcttcca cttcgcctgt atcagttaaa
     2641 ctgtttcgtt cttgaccaaa aggcatatcg aacaatccat acttccagcg acaagattga
     2701 gaacatccgc cacggtttgc atcccgcatc gacatatggt tggacaatgt gcatcgacca
     2761 gaataagaga tacacatggc accatggata aatgcttcga tttcggtatc cgtattttta
     2821 cgaatctctg ccacttcctc cattgaaact tcacgagcta aaacgacacg ttccaaccct
     2881 tcttctttcc agaattccaa tgtctcaaaa ttagttgctg aagcttgagt ggataaatgg
     2941 atcggcagac ctggggcttc tgtcgcacaa atttcgatca aagctggatc agaaacaatc
     3001 actgcagaaa taccgacatc acgcaaactg cggaaaaatt cccctgctcc atcttcattt
     3061 ccttcatggg tgaccatatt agcagcaacg tataccttcg catcatgttc ttttgcaaaa
     3121 gcaactcctt cagccatttc ttcataagta aaattacctg cacggcttct taaaccatat
     3181 gcattcccgc caatatatac tgcatctgct ccataatgga tcgcggtttt taacttttcc
     3241 agtgttcctg ctggcgctag gatttctggt cgtttcaatt ttctttttac tgtcatttct
     3301 ttcatcatac tccttatttg atagcagaag gatcaatata aaagaatcct gtatctaatt
     3361 cacggttttc aggatgcaat ttactgattt cgctagtcat tttttccgct ttttcttgat
     3421 cccagctacc ttctaaaatt tgtttttttg cttcggcgaa tagtcgagtg atgtttacga
     3481 atttttcgcc tggagtaaat aatccgtcca atttccatgt acagtaatga tgctgataaa
     3541 gttcattcaa ttcattcgag agattcagat cattgctggc aaaaatatgt gtgccatgac
     3601 tatcttcata aatagaataa tgtgtctctt cttttttagg ttccgagata aaaagacctc
     3661 tttcacgatc tttttgttcg tcttgtttcg tatagttata gtaattctgt agcagtggac
     3721 gtttggattg atggatgcat gtagcaccat acacaagggt ttctacagga atatcgagtt
     3781 tttcttccat cgccttcatc tcttcaaaag gaacttcacg agcaagtact gctccaactg
     3841 cgcctttttt cgcccagaaa ttgatttgac gtgcgctcgt tactaatgtt tcagcatcat
     3901 aaatatacgg tatacgcaat tcaggatttt ttttcatcgt ataaataata cctgtatcac
     3961 caactgtgat cacatctacg tttatttctt ttaaaaaagc caaatattca ggtacatttt
     4021 tcattttttc aggatgaatg atcccattta cagcaatatt cgctttttta ccatacttgt
     4081 gtgctaattc taccaattgt cgctgttctt ctcgtgtaaa tgaagcaggc agacgcaatc
     4141 caaattgctc ttcaccgaaa tatagtgtat ctgcacctgc agcaagcaac tgttctgctt
     4201 gttcaatagt ttctactgtg gttatgagtt cgatcataat tttctcctcc tacaacttca
     4261 taatagtagc atagctctaa aaactttcca atacttcttc tatttaaagt cggatttttg
     4321 aaaattattt cacaaaaaat caaaataaag ttcgatatat tctgtccaaa tataaaaaga
     4381 gattgtgaca aaaatcttgt cacaacctct taatcaagtc ttactattat aaaaattcag
     4441 ctacacaggc caagtaacta tagggaacct actgtttcct ttttaggtcc gcgaacattc
     4501 aatgtgatct taccttttga agcacctact gttacgtggt cacctaaacg aatttctccg
     4561 ccaagcaaag cctcgctcaa tcgatcttca atttcttttt gaagtgcgcg acggattgga
     4621 cgtgcaccat attcaggatc aaatccagct ttcccgatca catcgattgc agatggtgtg
     4681 attttcactt gaatatcttg ttctttcaat cgtttgataa tcgctttgct catgatttgg
     4741 acgattgtat ggatttcatc tttactcaat gaatggaaga cgacagtctc atcgattcga
     4801 ttcaagaatt caggtcggaa agctttcttt aactcttcta agatacgttt ttgcatggct
     4861 ttgtgatctt tagtaacatc ttggacattg aatccgacat ttttttcttc tcgaatttgt
     4921 gtcgctccga tatttgaagt cataatcatg attgtatttc taaagtccac ttttctacct
     4981 ttagaatctg ttaaatgacc atcatccaat acttgcagaa gtaaattgaa aacgtctgga
     5041 tgtgctttct ccacttcatc taagagaatc actgaatatg gtttttgacg aattttttct
     5101 gtcaactgtc caccttcatc ataccctacg tatcctggag gagacccgat taatcggctt
     5161 gtactgtatt tttccataaa ttctgacata tcaacacgga tcagggcatc ttcacttcca
     5221 aacatgactt ctgaaagtgc tttggccaat tctgtttttc cgacaccagt tggtccaagg
     5281 aacatgaatg aaccaattgg acgatcagga tctttcaatc cgcttcgtgc acggcggatc
     5341 gcacgtgaaa ctgctttgac agcctcatct tgaccaacga cacgttcatg cagaagtcct
     5401 tccagttcta acaagcgttc gctttctttt ttctccaact gctgtagagg gacaccagtc
     5461 cactccgata caactgtagc aacatcttct tctgttacac gatcagaata accttgctcc
     5521 tgttttgctt caattgcaga aactttttgc agctttttac gtagtgcttt ttcttgtctg
     5581 cgtaattgag cagcattttc aaagtcttgt ttttggatcg ctgcttcctt ttcttgtact
     5641 agttcatcga tttctagttt gatcacagtt gattttgtta agtgatcggt ttgatccaaa
     5701 cggactttcg ctgctgattc atcgatcaaa tcaattgctt tatcgggtag ttgacgagaa
     5761 gtgatataac gaacagaaag attgactgct gctcgtacag cttcatctgt gatctccact
     5821 ccatggtgct cttcatatct tgtacgtaat ccttgaagaa tcacttctgc ttcttcagga
     5881 gttggctcat ctacttgaat gcgagcaaaa cgtcgctcta atgcagaatc tttttcgata
     5941 tatttttgat attcatctag ggtcgttgct ccgatcgttt gtaattctcc tcgagctaac
     6001 gccggtttca agatattcga agcatcaatt gctccttctg ctccacctgc accaattaag
     6061 gtatgcaatt catcaatgaa taagattact tgaccatcat tatagatttc gtcgatgacc
     6121 tttttcattc gatcttcaaa ttcaccgcga tatttcgtac cagcaaccaa agatcccata
     6181 tcaagcatca tcaggcgctt ttcttgcatg tcttctggca cttcacctaa aatgattttt
     6241 tgagctaacc cttcagcaat ggctgttttg cctacaccag gttcacccac cagtacaggg
     6301 ttattctttg tccgtcggct aagaatctgg ataagccgtt tcacttctct cgaacgacca
     6361 acaactgggt ccagcttgtt ttcttttgct aatttcgtca aatcacgtgc taatgaatct
     6421 agcgtaggtg ttccttgcgg tgtttgccgt ttatcttgta catttacatt tctacgacga
     6481 ttcattcctt ttccagcaga atcaccagct agccccattt tttgtttcag aacttgacgg
     6541 gcttttgaaa ggctgatacc taagttgatc atgattcgag tagccatgat actttcatct
     6601 tttaataagc tcatcaaaat atgttctgtt ccaacttttt gagaaccagt tttcttcact
     6661 tcgctgctag ctaaagcaaa aacttgtttc attcttggtg aatatggaag aaagactcct
     6721 ttagggtaag aacggatacc accataacca atcagatgtt caatttcttc ataaatttcc
     6781 gtttcattca aatccatttc acgtaatact tttccagcaa ttccattttg ctcgaccact
     6841 aaggctagta aaacatgttc tgaactaact gattgatgtt tcaaccgttt tgcttcttct
     6901 tgtgcaattt ctaatactgc ttgggctctt tcagtaaaaa gttcttccat ctaatctcac
     6961 ttcccatctt cataacttaa acgttccaga aatgctcgga gaatccgagc acgtgtatga
     7021 tcttcatatt cattgaaatt caatgtgttt tttgcaattg cactcaacat caagtttcct
     7081 tctttttttg taattatttg gtcttcataa agtttttgaa taatagcaaa agcatttttc
     7141 tcactgattg tatcatcaaa taactgattt atctgctcaa gtaattgttt tttatctgaa
     7201 atccttactt ttgcaattcg tatataacca ccaccaccac gtttgctttc tactagataa
     7261 ccacgttgga tggtaaaacg ggtattaatc acatagttga tttgtgaagg aacacagtta
     7321 aagaggtctg ccatttcaat ccttcgaatc tcgatcattt cactattttc cagaattttc
     7381 tttatatatg attcaattaa atccgaggta ttctgatgtg ccatcttatt cattcccttt
     7441 gactaatatt gaccttaatt ataacgaatt tgataataga aagaaagaag aacgcttttt
     7501 gttaaataaa tcctttagaa tcaacaaaaa aaaatacgga atatactatt ccgtatcgac
     7561 gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta gtcaaacact ctatccagtt
     7621 gagctatggg cgcatattca atttaaaaaa attataaatc atttgaaaac aaaaagcaag
     7681 ataaaaaaaa tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc
     7741 tctaccaagc tgagctactt cccgtttaaa aaataaaaaa gac
//
LOCUS       DAYYKN010000128         7761 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000128 DAYYKN010000000
VERSION     DAYYKN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.130"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..212)
                     /locus_tag="ACHBB7_002378"
     CDS             complement(<1..212)
                     /locus_tag="ACHBB7_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM9210284.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLE"
     gene            complement(209..727)
                     /gene="msrA"
                     /locus_tag="ACHBB7_002379"
     CDS             complement(209..727)
                     /gene="msrA"
                     /locus_tag="ACHBB7_002379"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9210285.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(747..1460)
                     /locus_tag="ACHBB7_002380"
     CDS             complement(747..1460)
                     /locus_tag="ACHBB7_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM9210286.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1463..2359)
                     /locus_tag="ACHBB7_002381"
     CDS             complement(1463..2359)
                     /locus_tag="ACHBB7_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9210287.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2517..3359)
                     /locus_tag="ACHBB7_002382"
     CDS             complement(2517..3359)
                     /locus_tag="ACHBB7_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9210288.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3491..4144
                     /locus_tag="ACHBB7_002383"
     CDS             3491..4144
                     /locus_tag="ACHBB7_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM9210289.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4207..4734)
                     /locus_tag="ACHBB7_002384"
     CDS             complement(4207..4734)
                     /locus_tag="ACHBB7_002384"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM9210290.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4754..5701)
                     /locus_tag="ACHBB7_002385"
     CDS             complement(4754..5701)
                     /locus_tag="ACHBB7_002385"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM9210291.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(5713..7596)
                     /locus_tag="ACHBB7_002386"
     CDS             complement(5713..7596)
                     /locus_tag="ACHBB7_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9210292.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
BASE COUNT         2012 a         1585 c         1360 g         2804 t
ORIGIN      
        1 ttttctaaat atttttccca tgcttcgtca aagatatcca tggacggcaa atagtcgaca
       61 ttcagttcca gataattaga aatttcttcg tagctagagc tgtgcttagg aaattggatg
      121 tctttaccgg cttctgtagc gaaaacagat aaagcatcat gtgtgccacc gcctcgcagt
      181 gtcattaaat aatgaaaaaa gcttcgattc acccttcgtt cctccaattt tcttcgataa
      241 aggcagcccg gcgtgcatgg tttcttttaa aattttcagg atgcttttta taatactctt
      301 gatggtcttc ttctgctaga taaaatggtt gggctggttc gatttttgta acgattggct
      361 gttcgaaacg tccactttca gctaactgtt tcttactttt ttcagcaatg atccgctgtt
      421 cttctgtcaa ataaaagatc accgggcgat aattgtcacc gcgatcctcg aattgtccga
      481 aagcgtcagt tggatcagtt tgctgccagt aaagttctac taattgttca tatgaaatca
      541 atgttggatc atagataatc tccactgctt ctgtatgtcc agtcgtttgg ctttttactt
      601 gttcataagt cggatctggt acatgccctc cagtatatcc tgacatcact gtatggatac
      661 caggaagcgt gtcgaaaggc tggatcatac accagaagca gcctcctgca aatattgctc
      721 gttccattat gattccccct ctaatactac tcatccgatg attttttatt ttcttttgtc
      781 ttgctttcag tactgctggt gctagttttg ttggtatttg agttgtttga agcatcagag
      841 gtatcttttg gcaaatatag actaaaccga atgtcatcat ttactaagtc gattttatct
      901 gctttaatat acatcccgtt ttgcattcgg aattgatcca acctaagcat gatagtcaga
      961 tcatctgtat tgacttcgat ccaatctggt aatttatagt ttctcttgat catattcatc
     1021 acatctttga ttggcaaact caatgttccg atagaaagac ttctagcttt tagttggaca
     1081 tttccgtttt ccatcacata cggatcaaaa tataaataga aatcgatagg aaaacctagt
     1141 acttcgaaag taccattgag tagtgcttca ttttccaaat aaaatttata ttcaatatca
     1201 ctatcttttt gatactcact aaggaaaaag ctgatgattt gattgacttt ttctttattt
     1261 gaattgatcg ttaagacagg atctccttgt ctttccgtaa gagctggtat ttctgggagt
     1321 tctggttccc gattagcaaa aatacggctg cctaaaaata cggtcgaacc aagtactacc
     1381 ccaagaagga taaggaaggc gaatttccag taatttgttt tctttgtttt gtttgaagat
     1441 acgtcagatc ttgttttcat gtttattgat catcctcctt ctctaaccat tggttttgtg
     1501 tctcgatcag ctgatctctc actgcatttg ccatcaattg ataacctaaa ttatttggat
     1561 ggaaatgatc ttcttcatag agcacattat ttttgatgcc atttagattt tctgtcgtgc
     1621 tagttgtttg atctgttcct tcttctgtga cacctacttc ctgattcaat ccttgataaa
     1681 gcaaatcatt gattgggata aagtagcagt tcggcatttt ttttgtcacc gtttctgttc
     1741 ctttgttcca attgttaaca attgtctgca tgtcagtgat ctcaggaaag ttcaaataaa
     1801 atggattgta gatgcctaaa atgtaaatag gtgcggtttt attgagggat cgaaattctt
     1861 ccaatagttc tgtgacattt tcctgatagt ttttcagtgg ctttttaaag gaattgaccg
     1921 ttaacccgaa taaattattt tggataactt tcatcaaatc attaccgcca acagtcagtg
     1981 tgataaaatc agctgttttg atattgtttc gaatttcttc ttttgttttt actcgcttta
     2041 agatctggtc acttcgttca ccatttacac catagttttc tgtttcaacc gcggtcaaat
     2101 tataacggtc ttttagatca tttgcgacaa ggggaacgaa tcctccttgc ttggtttgat
     2161 ctcctactcc ttcagtcagc gaatctccga tagctatgta gtgaagtttt tcttttttat
     2221 ttggatcttc tctcacagta gttactggtt tcattagtgg gccagctttc ggtatagaag
     2281 cagctaaacc tgcatagatg atgatcgcaa ttaataatgg gaacaaaacg aggatcaaat
     2341 gtcgtttcgt taatttcatt ttttcacctc ttgtattcga ttggttctaa tgttatcaaa
     2401 aattttatca aaaaggtagt ctttatgaaa aaagaaaaca attattttcc taattttata
     2461 aagaaaaaga gaataagctg caagcctttc aggctgaggg cttatcctct tttcgactaa
     2521 tctacataat acatgattgc aaacgcgtct ttaccagtat gcgtagcgat aaccggattc
     2581 gtatgcagta cagggatatg catgtccggg aataattctt gcaaaccctc tttgaattct
     2641 tgcgccaatt cttttgcatc cgcatgtgaa ataccgattt gtcgcacatt tggcaattct
     2701 ttcagttcat ttttcaaatt attaaaccat ttatcgaacg ttttctttcc tcgcccctta
     2761 gttacaggga ttaattcaga atgggcgaaa ttcatcacca cgcgtatgtt caaaacacta
     2821 gacagcaaac cagttgttcg gctgattctt ccacctttta ccaagttatc tagtgtggat
     2881 attccgatgt ataattttgt attgtcttgt acttttttga cttcttcaag gattttctct
     2941 actgttttac cttcttttgc taattcagct gctcgaatta cctgaaaaga taatccttgg
     3001 tcagtggtat cactgtcgat aaccgttact ttcgtttttg ttagattact ggcttgtctt
     3061 gctgcttcta ctgttccact caagcctttt gtcatatgga tcgaaatcac ttcactgccg
     3121 tcttctccta gctcatcata catcgagatg aattctccaa ttggtggttg gcttgttttg
     3181 ggcagatttt ttgctttcgc catcatttcc ataaatcttt cgccgtctag ttggtcatca
     3241 tcgggatata ctgttccgtc gatcatgatg gatagcggca tgacacgaat gcctaaagca
     3301 tcgcgtacat ttttttccat cgtacatgaa gaatctgtta cgatttttac atttgtcatt
     3361 attttctctc tctctttctt taacaagatg ccttattcat gttaaactac atgtaagaca
     3421 atttctattt ttagtataac agacaaataa tgatttttca ttaattacac ctagtaaagg
     3481 agccgttttc atggaaaaaa cgaaatttaa tcgaacgtat gaaatattaa atgaagtgtt
     3541 taatgcagtc acccatggaa ttgggactgg attgagtatc gcgggactag tggttctttt
     3601 agtaaaaggt gcccacttgc attcctccct ccatattgtc tcttatgcta tctatggatc
     3661 gatgatgatt cttttatttt taacgtctac tttgtttcat tcattgattt ttacaaaagc
     3721 aaaaaaggtg ttccaagtat tcgatcatag ttcgattttt cttttgattg caggaagtta
     3781 tactcctttt tgtttgctga gtatccgagg atggctaggt tggactttat ttgtattaat
     3841 ctggttgttt gcgattcttg gcattatata taaagcgttg acgttgcata aacgggagag
     3901 agtatcaaaa gtatctacga tcatctatat ttttatgggg tggctctgtg tcgtggctgc
     3961 tgttcctctt tatcactctc ttggaatgac tggtgttatt ttgatggtag caggcgggct
     4021 ttcttacacg ataggcgcat tcttttatag cttgaattct atccgctata tgcatgttgt
     4081 ttggcatttg tttgttatgt taggtgcagg ttttatgtac ttctctgttt tattctttac
     4141 ttaataaaaa aaataaagct atggatggga cagctctaaa taacagctag tctatccata
     4201 gcttttttat atcgtcaatt cgcctgtccc gctttttaca ggattctcat tacgctgata
     4261 cgtttcaaat tgatgcggat aacgattttt ttcatccatt tctccttgac tagtattgat
     4321 cagtgaccaa tcagaccagt caacaggtgg aaaataagca tctccatcga atacatgatg
     4381 gatgactgta cggtataata tcgtacaaaa tggcagaaat tcttgataaa cagctgaccc
     4441 tccagcgatg aaaaagatac cgtctgtttc tttttccaga gcaagcacgt cttccacact
     4501 atgcatgaca tgcacgcctt ctgctttata ggaagaatct ctcgtcaaaa caatcgtctg
     4561 acgattcggc aacggacggc tacccatccc ttcgaatgtt tttcgtccca tcactagtgt
     4621 atttgcttct gtcatttgtt tgaagaattt cagatcattt ggcaaacgcc aaggaagacg
     4681 gtcttctttt ccaatcaatc cgttttcatc ctgtgcccaa attgcagcta acattttctc
     4741 atcctttctg attctaaaca gcgataggag ctttgatcgc cggatgtggg tcatatcctt
     4801 ctaattggat atcttccatc tcaaaatcaa atactgactg cttttcctga ttcaaaacta
     4861 aagtcggaaa tgcgcgtatt tctcggctca attgttcttt catttgttcg atatgattga
     4921 tataaagatg agcatctcct aatgtgtgaa caaattcgcc aaccgctaaa cctgtttcat
     4981 gtgcaattag gtgagtcagc aacgcataac ttgcaatgtt gaacggaacg cctagaaaca
     5041 catccccact gcgttgatac aattggcagc ttagccgtcc ctcttgaaca taaaattgaa
     5101 acatcgtatg acaaggaggc aaagccatgc taggaacatc ttccggattc catgcagaaa
     5161 cgatcaatct tctagaatca ggagtctttt tgattgcttc gatcacttgt ttgatttgat
     5221 cgataaagcc accgtctttt gtttcccagt gacgccattg atagccgtag atgtgtccaa
     5281 gatcgccgta tgtttcagca aaggaatcat cggttaggat cttctcgcaa aaaagcttgc
     5341 tctgcttttt atattcttca ttgaattcag gatctgtcaa acatctatgg ccaaaatcag
     5401 tcatatctgg tccattatag tctggacttt tgatgtagcg ttcaaatgcc cattcatccc
     5461 aaatatggtt tttattttgt aatagatagc ggatattcgt atctcctttt aaaaaccaaa
     5521 gcaattcgct tttgatcaat ccaaaaggta ctcgtttcgt tgttaacaaa ggaaaccctt
     5581 tttgcagatc aaaccgcatt tggtatccga ataagcttaa tgtgcctgtg cctgttctgt
     5641 cttctttacg atgcccttct tccaacaatt ttcttcctaa gtcaagatac gcttgttcca
     5701 tcctttttcc tcctaatctt ctacccattc actcaagtat tcccaccgtg ccattttttc
     5761 ttcaagttgt gcttctgttt cagatacgtc gttttgcaat tcttgtaatc gagtgaaatt
     5821 atctccttga tgattcatct cttcttggag caatgagatt ttttcttcaa gttctgcaat
     5881 ctcatcttca atcgtttccc attctttttg ttccatgtaa gataatttct tcttttcagc
     5941 aggttccttg ctctgtattt ttggtttcgc tttttcagca ggctgctggg tctctttttg
     6001 cttcagtaaa taactggaca tgtttccgta ataaagttct tgttttccgt ttccttggaa
     6061 tactaataac ttatctgcta ccttgtccaa gaaataacgg tcatgggaaa cagtgatcac
     6121 agcacctgga aaacttctaa aataatcttc caaaatcgtc aatgttgcta tgtccaaatc
     6181 attggtcggt tcatccaata ataaaacatt aggttgctga atcagcagtt tcaacaaaaa
     6241 taaccggcgt ttttctccgc ctgaaagttt gcctatcagt gtcccatgca taaatcttgg
     6301 aaataaaaat cgttcaagta attctgctac tccgatttga ctgccatctg ctgtttttgc
     6361 ttcttcagca gcttcttgta agtaagcaat catccgttta tctggtgcca tttcttcatt
     6421 ttcttgtgta taatacgcaa gatgaaccgt ttcaccgata gacattgtac cgctatccag
     6481 gggaattctg ccagctagaa tattaagcaa tgtcgatttt cccgcaccat ttttaccagt
     6541 aatacctagt ctttcccgag attgaatcaa taaatctaaa tgttctagca gtgtttggtc
     6601 atttatacgg taactgccat ctttgatttc cagtactttt ttccctaaac gttgtgtcgc
     6661 taggttcagc tctaaatcat cttcttggtt tacttggtac aagttctcct ttagatcttc
     6721 gaaacggttg atccgtgctt gctgcttggt gccacgtgcc tgtacgccag ccctcatcca
     6781 agctaattcc tgtttataaa gccgcttgcg cttttcttct tgttctactt ctacacgatc
     6841 acgttctgct ttttctaaga cgtaagcttc ataatttcct ttatactcgt ataattttcc
     6901 aaatgaaagt tcgaagattc ggtttgctac tctatctaga aaataacgat cgtgggtaac
     6961 catcaatagt gcgccacggt attgtttcaa atagttttcc aaccactcga tcgcttcata
     7021 atctaaatgg tttgttggtt cgtctagtaa tagtaaatcg ggttcatcaa tcaacacttg
     7081 cgccagacta actcgttttt tctgaccacc tgataaagta ctgatttttt tatctaattc
     7141 actgattcct aatttttgca aaataatctt tgcgttggta tctgtagtcc aagcatcttc
     7201 tttattcatt tgttcttctg ctttagcata acgtttttgc acatcagggt cttctccgtt
     7261 ttcccctagt tcgatcaatg cttcctcata tgctcgaaca gtttggatca aggggctgtt
     7321 tccttgaaag actgcttgga gaacagttaa ttcatcggaa aattcagctg cttgagaaag
     7381 ataaccaata cggtaatcag ttggataaaa cactgtttgc cgatcaccgt cgctgctatc
     7441 gataccggca ataatattta agagacttgt tttacccgtc ccgttcgtcc caatcaaccc
     7501 gatacggtct ttttcgtgga ccaaaaaaga aatctggtca aacaaatctt tttcaccata
     7561 agtttttttg aggtcagtta cttttaattc tttcaacatg ttccctcctt atcttggata
     7621 ttgtatcata aaaatagtga acttcaggaa aatttttttg gtatttgttc tttttgtgtt
     7681 ctcaaaaaaa gaacaggacc gattcgatcc tgttctgata tgttttgttt tttataaaac
     7741 ttgaaaattt ttaccttttt a
//
LOCUS       DAYYKN010000129         7611 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000129 DAYYKN010000000
VERSION     DAYYKN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.131"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..740)
                     /locus_tag="ACHBB7_002387"
                     /pseudo
     CDS             complement(42..740)
                     /locus_tag="ACHBB7_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            819..995
                     /locus_tag="ACHBB7_002388"
                     /pseudo
     CDS             819..995
                     /locus_tag="ACHBB7_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1171..1740)
                     /locus_tag="ACHBB7_002389"
     CDS             complement(1171..1740)
                     /locus_tag="ACHBB7_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9210293.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1814..2137)
                     /locus_tag="ACHBB7_002390"
     CDS             complement(1814..2137)
                     /locus_tag="ACHBB7_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210294.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2291..3103)
                     /locus_tag="ACHBB7_002391"
     CDS             complement(2291..3103)
                     /locus_tag="ACHBB7_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210295.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4305
                     /locus_tag="ACHBB7_002392"
     CDS             3430..4305
                     /locus_tag="ACHBB7_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9210296.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4319..4642
                     /locus_tag="ACHBB7_002393"
     CDS             4319..4642
                     /locus_tag="ACHBB7_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210297.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4639..5391
                     /locus_tag="ACHBB7_002394"
     CDS             4639..5391
                     /locus_tag="ACHBB7_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM9210298.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVCLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5381..7552
                     /locus_tag="ACHBB7_002395"
                     /pseudo
     CDS             5381..7552
                     /locus_tag="ACHBB7_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2263 a         1507 c         1375 g         2466 t
ORIGIN      
        1 agttcaactt ttggggttca gttcaaagag ggggggattt tttatcgttg tggaatattt
       61 aaataccaac gcttgtcatg aaaatcttgt gctccaccct tagtatttcc ttctggatcg
      121 tttgtcgcac gcatcatgac gtatactttc ttattaggga aatcacgcat attgaaagat
      181 acatgatagc caacatttcc taaagtatta taagcttgat ttacgtctgg tctataaatc
      241 ccatcagctc ttactctagc tagttctttt ccagtattgt agtccataat gaaaatatac
      301 tcgtatttat aattagcaat gtgccatcca gctacatgca agtttgcgtt ttcgatttct
      361 ccgaactgat caatgtgggc gtaatttgtt ccatctgtca gcgtagaatt tgcagcacct
      421 gctcgagttg gatcaattac tggtttatcc tccgaagtag ttggattatc atccgtaaaa
      481 ccatgagcca aatcataggc tagtttttct ttacttacac ccatttcaga aagataaccg
      541 taaggatctg tatgatcgcc ccaaatattt tgtgttaccc ataaatgcga tttgattcct
      601 ggttggttat aaggagtgtc taatgttaat ggaataccat atttcattgc tgaatctctt
      661 gccaattcaa cgtatacttt atagttcttt tcaaaagttt ctttatcatg tgtatgttgt
      721 aactcgattt gcacaggact tttggctcac gaggttgtcc tactctacat cttgctgata
      781 gatatgctga aaggaaaaca ccaacattat gttttgcttt ttcagctaga acgagacaag
      841 caggaatacc tttagaagat atcaaaggtt ttttaggtca taaagatgtt tccactacac
      901 aagtctatgc ccatatttct cccgaagtga aaaaaagatc aatgaatcaa ctagaaaact
      961 atatagaaga gcaaataaaa aagcactcaa attgagtgct tttttaagct cactatcact
     1021 aaaactatca ctaattttat ttataagcga acatgtttct atcactgatt ttttaaatct
     1081 agcgaaaaac ccttatctta aaggcgttcg ttcaattctt tggcaaggtc ttcaaagcct
     1141 ggcttgccta gaagtgcgaa catggttttc ttatagctca tgtagaaaaa ttttcttgat
     1201 gatgatttta tccatcaatt gagattgact gttttgctct cctatgattt cagctatgat
     1261 tgtccgattt tgcggttttc ttcttaactg gtagttgatt ttcccagttt ccatcaactc
     1321 gctaagcata tccacgactc cgccaaatcc ggtctttttc gtaataaaac ctagtgctac
     1381 cattcgttca tctagatttt ttgctgtaat cgtcacacca gtaatgagcg agtgctgtaa
     1441 tgtttgggcc agctcttcca tcccttcagc attggatacg acggaagctg ttttgttaat
     1501 aggttcttcg tttatttctt ttggttcttt cctctccact ttcactgttt gacgaaggac
     1561 atctagttct tgcaagagcg tttcgtattg ttcttgggtc agcatcacgc cagctacttt
     1621 ttcacgatta aatatatata cgccagttcc cgcgtctcgc gcttgagcaa atacatccat
     1681 cggcgatcgt ttcacttctg tgatcgaaga tgtgggaact tctaatttct ttaattccaa
     1741 tggatttcct cctttactaa tcgcttttta ttatagcaca gactgacttg ttttgatctt
     1801 ttttccaaag gttttatcgg tttttgccta accagtaatt gatatcggta agaagtcttt
     1861 cgataccaaa tgaaggatgg tacagatacg ttttttgatc ttcgttatgg tctagtggaa
     1921 aaaaatgatc gatgatcact agatctgcat accgaggcgg cttatttcga gtacatacat
     1981 gtatagagat ccgttttcga tgtcttagcg ttttgttcag taccccagca agtcttcttg
     2041 cttcttggtg ttcatagcga aaaaaaaaga cgattttcac ttcaggaggg tggacatcta
     2101 aataccctcg ttcctggagt gttttcagta aaagcatccc ccactctact gcggggtgtg
     2161 caagtgcttg ttggatcgct agccgaaatt gatatagttt atctttggaa ttgggaaatt
     2221 tttctaatgt ttcctgataa taacgaactt cttctatcag ccggtcatgg ataccaatta
     2281 cgtaacgaag tcattcttgg taaagtactt ttgtcagctt tgatttgctt tcgctctcaa
     2341 attcgctatt cagccaaaac agcgaatcaa gcttttcagt cacaatcgtc aatttccgat
     2401 agtcttgtgt ctcttctatc cgtttccacc gcttttcagt aatctggtta cagctcaagc
     2461 gcaaaattgc tagataattt ttttctttta ttggcaaaat ctgctcctct ctaaatagca
     2521 attcaggaaa caattcaaag aatagtgttc gactgtctcc taaaatctcg atttctgccg
     2581 gatcctttct ttgcttcaat aattggatga acatctcaga aaaggactgc tctccatcat
     2641 ctgcaaaaaa atgcggaaaa tctttcaaaa agcaaaacag cagatatctt acttttcttt
     2701 ctgaaccaaa gatctgtgct tttttataag aaaaagaaag gtggtactcg ccaaaccact
     2761 ttctcaatcg ctcttgcgta gaacgatcgt acttctgctc gtaaggttca ttcaatagca
     2821 gatttttcag taaacaaaac gggggtgatt ggatcagaaa aacaaacaag agataaggtt
     2881 cgctatatcc ttgatcataa actaactgaa cggttttctt atgaagaatc agctgaatgg
     2941 gacagtcgct ttccgccaaa cgtttcagat gccgatccac tgttttaaca tcgattttca
     3001 gttcttcagc taaatcagct tttcgccaga tcaaatcttt cattagattg attagtgaaa
     3061 ataaattctg tgcatttttt gataatagtt tttgccatct catcgatcat ttctcacctg
     3121 atttctaaac atattgtttg atcaaaatgg gttcactcct aaaaagataa aaaactccct
     3181 ttctatttat ttaaatacgc aaatcttcct cattattttt tattttactc aatttaatcg
     3241 tattctcatt tcaaaacgaa tgtactttaa aaaaacataa taagatcact ttgcttgtag
     3301 gatgtattcc cttccattac aatgaaataa atagcgattt tatgagaagg tgatgtgaaa
     3361 atggaagaaa aaatagaaga atccaaacaa aaaatcgatc agttgatata agaattagaa
     3421 aaagcggtca tcggcaaacg ttcaactatc caattgatga tcgtggccct attagcaggt
     3481 ggtcatgtct tattcgaaga tattcccggt gtaggtaaaa cgttactggt caaagcacta
     3541 gcaaaagctg tacagggaac cttcagcaga gtccaatgta ctcctgatct gctgcctagt
     3601 gatattcttg gtttttccgt atacaattca caatcaaaag agtttgaatt tcgtccggga
     3661 ccgatcttta ccacgatctt gttagcagat gagatcaatc gtacgactcc tcgtactcag
     3721 tcagcacttt tagaagcaat ggccgagaat catgcaacga tcgacaacaa tacataccct
     3781 ctagacaatc atttttttgt cttagctacg caaaatccaa tcgagtacga aggaacatat
     3841 cctttgccgg aagcacagct cgatcgtttt ttattccgac tacaaatcgg ttatccttct
     3901 tttgatgacg aacttttgct acttttggat aaattcgaaa aagtgttgga taacttaaat
     3961 gaagtattga acagttatga aatagaagaa ataaaaacaa atgtggcgaa tgtctttgtc
     4021 agtcccgaag tggcatctta tgccttgcag ttagtgtcag caacaagaag tcatgctgcc
     4081 atccagcttg gcatcagtcc acgaggaagc ctttccttta ttcaggcagc aaaagcttat
     4141 gctttaatcc atgggagaaa ttacgtcacg cccaaagatt tgcaggattt agttccttac
     4201 gtgttttccc atcgcctgat tttttacgat cgttcattga aggaagacga aaagaaacaa
     4261 atcattcata ctcttgttgc acaaattcct attcctgtga ggtagattga ggtagacaat
     4321 ggaaaaaatc aaagattatt tcattaattt tgtgttattt ctggtatatt tcatcattgt
     4381 gctttttgct cttgtattca acaattcttt aggctggttc ttgttggcct tttttactct
     4441 ttatgtatca tggagcttga tttcattaag cagctttcta aagaaacttt cggttacttg
     4501 tacattgcag ccactcgttt atcgcaaaaa aagtatgcag gttatttttt ccatagcaaa
     4561 aaagagaact ttttctttac ctctccctcg tttacaaatc ggcttcccag caatctttgc
     4621 agaaaaaaac aagagatttt gattcttaca aataaaccgc aagaagtcca attgctttgg
     4681 cagccaaaag aaagaggatg ccatgaaaca ttaactgtca tttttacagc ctatgatagt
     4741 ctaggacttt tcagaaaaag tgcaaaaaga gagatctctt ttcctgtgac tgttctgcct
     4801 gctttttgta agattcaagc agaaaaatta cagagagttc tggaaaaaaa acagcagctg
     4861 aacgcgtacc aaagaggtct tgattttcgg gaacatcgcg cttatcgtcc aggtgattct
     4921 ttcaaccgaa tcgattggaa attgtctgct catactcaag agtggttata ccgtgaatac
     4981 gattatcaag aagaagaatg ttctcctctt ttttgctttt ggggttcgcc cagtccaaaa
     5041 tttgaacctc tgctagactt gtatttttct ttatggcatt tgagaaaaga aaagcagccg
     5101 gaacttctga tattaggcaa ccgcgccttt catggcaaag accccggtca tacctatttt
     5161 gcttctttgg aagctggcga tgagaaagct tttttcgcac acttgaaaaa atatgcaaaa
     5221 aaacaaatcg tcctctttgt tcctgagaat tctcctgaag tctcagaagc tattgaaaca
     5281 ttgtcaaaag atcgaacggt ttgtcttgtt tatttcgtag aaaaacaact catggtccaa
     5341 gaaaaagaca agatatgttc attgccagga ggtgaatgga ttgacgatta aacgggaaaa
     5401 atggctgcgt ggcgtttact cctttgtgat actaagtgta ggtgctccga tatttgtagt
     5461 agctaatcaa ttgcaaaaca gctaccttct tatcctaact attggtgtcc tatgccttat
     5521 tgtctgctgt attcctaaaa aatgggtacg tttgctgctt tctttgccag taatgatagg
     5581 ttgtttgttt ttatattttc cttcgaagac attttccata ttatggatct tacagttttt
     5641 ttcgtcagtc acagaggaat tcttccatct atcccaaggt aaactttatt atttgccaga
     5701 aaagacagct ttttttctca taatgttgct gatctattta tttattacat tgaccgtgga
     5761 ttacgactat tggtacgcac cctttctttc tttgatggct tacttcatga tgcttaccgt
     5821 ttttcggaaa aaagatttac tgtacgaaat gattgcggta gtaactgttt tgttcgtgta
     5881 tctggcagca aggcaatttt tctccatccg gttgttgtcg ggaaacactc gattccagtc
     5941 attgactaca gttctgctta tttgtttctt aacttttagc gctatcttgc ctttatatct
     6001 tccgaaagta caccaatctc tcgaggaaac gagtgaaccg atccgagaat atcttaatga
     6061 tgaagggctt tatgacacac tccatgagta tcaattaacc ggaagtgcaa gaacaggctt
     6121 cagtgagaat gatgaacagc ttggtggacc tttatatgat aatggcgaag ttgtttttac
     6181 agcgaaccaa aaaggaaatg gctattggaa gatcgaaaat aaaaatcatt acaccggttc
     6241 tggatggata gaagatcagg acgcacccat ccaaccaatc caagaattcc ctgttgacat
     6301 cacgagctac caaaccgtta tagaagacaa agaaaacatt acattgaccc taaaaaaaca
     6361 agttttctta ccttatcctt atggaaatac taccttgact gatttttcat tagctagtgg
     6421 acaaacgctt tactatttgc ctgaaagcca gcgattcatt cctgtcccga atgaatcgac
     6481 acgttctgca actttacaaa cagaaaggtt agtttttacg agagaagaat taaaaagcag
     6541 tcagttaaaa gctgatgatt tagaaaatca gcagacaaat cttcaattgc caaaaagttt
     6601 gcctaaacgt attgaggact tggcggtcga attgaccaaa aatgaagcaa ctttaattcg
     6661 taaagtcgaa gctgtctaac agtatttaaa aacaagcgga aatttccgct attccaaaac
     6721 ggataccggg catactccaa aagaacacga ttatgtggat tatttcctct ttgattcaaa
     6781 agtcggctat tgtgacaact tttcaacttc tatggtcgtc cttcttcgaa cacttggtat
     6841 cccagctcgc tggacaaaag gttttacaaa tgggctcaga acaggcacag ataatcaagg
     6901 gaatgaggaa tacagcattc tcaatagtca tgctcattct tggccagagg tatactttcc
     6961 tggatttggc tggcttccat ttgaaccaac cccttcattt gccaatccaa tgggttcaca
     7021 accagttgct gaaacaaccg aaaccgtcca gcctgctatc gaacaaccaa atgaaagcga
     7081 aaatatggca gaaactattt ccagtgagag atctgaggtc attccatcag aagaaacaac
     7141 actcacacca gcttataaaa tcgtcttatt cagccttgga gtgtgtttat ccgtcttgtt
     7201 gttctttcta ttgttattca gaaaatctct gcgctggaca agttatctta tctatcttca
     7261 tttttttgct tctactaata cgattgcata tcaaaaactt cttaaccctt tggaaacatt
     7321 ggtaccgcgc aaacccgcag aatctttgca ggaatatagc ctgcgaatag aacaagaatt
     7381 caagctagat ctgcgtttca ttcgattaac tactcaatat gaagcagaaa tatatggacg
     7441 acaagcagat ccggtaaaat tcgataaaaa aacctattta gaaataacgc atataataag
     7501 aaaaatggta tacaaaaaaa ttctgacaaa agggagccgc ttcaaaaaat aagataaaat
     7561 ttctggagaa tctacttctg gaatgccgag aaccagctga ttgagtatcg a
//
LOCUS       DAYYKN010000130         7422 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000130 DAYYKN010000000
VERSION     DAYYKN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.132"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..312)
                     /locus_tag="ACHBB7_002396"
     CDS             complement(220..312)
                     /locus_tag="ACHBB7_002396"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9210299.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
     gene            complement(583..1563)
                     /locus_tag="ACHBB7_002397"
     CDS             complement(583..1563)
                     /locus_tag="ACHBB7_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM9210300.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(1576..2454)
                     /locus_tag="ACHBB7_002398"
     CDS             complement(1576..2454)
                     /locus_tag="ACHBB7_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFM9210301.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(2470..3270)
                     /locus_tag="ACHBB7_002399"
     CDS             complement(2470..3270)
                     /locus_tag="ACHBB7_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFM9210302.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            complement(3545..3694)
                     /locus_tag="ACHBB7_002400"
     CDS             complement(3545..3694)
                     /locus_tag="ACHBB7_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210303.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            4662..4988
                     /locus_tag="ACHBB7_002401"
     CDS             4662..4988
                     /locus_tag="ACHBB7_002401"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9210304.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            4996..5904
                     /locus_tag="ACHBB7_002402"
     CDS             4996..5904
                     /locus_tag="ACHBB7_002402"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM9210305.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            6530..6784
                     /locus_tag="ACHBB7_002403"
     CDS             6530..6784
                     /locus_tag="ACHBB7_002403"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFM9210306.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
BASE COUNT         2244 a         1472 c         1235 g         2471 t
ORIGIN      
        1 ttcgccaaca caatttgacg aagagccttt tctttcagga agacttcggt aatttttagg
       61 agttctacct attcttttaa ccgtttattc tctttttcta agcgtgtaat ttcatttctg
      121 cgaatggatt tcatgacttc ctctaccgag aaaagtacgt tatccatatt tttcaacttc
      181 ttgaactcat caatacacgc tattagagcg agcttctagc tatagactaa cttctttaac
      241 agaaaaccct tcttccgtta ccagttttac agcggtaatt ttaaatattt catcaaatga
      301 acgtcttggc ataatccaga tcttcgcttt ctcttaattt ggtgtcaatt ttagtataac
      361 ccgtccaact tattttttaa ctaagtgccc ggtttttctt gacaactcca cccaaaagtt
      421 agattttttg gtctaagttt tagggtcgct tcattactag aattcaacaa gagatgtttt
      481 ttattttatt taaattatgg ggtcagtttc catttgatta caaacttctt tgttttcttt
      541 ttttaaacgc aataataaag gttttaattt cagccttttt aattagaaaa tcaattttcc
      601 caaattattt tctttagctt tcttaacgat ttctgtcgca actaccacat ctaatacagc
      661 cgaaccaact gttttgaaga tcgtgatttg ttctccactt gtccgaccga tcaattcttc
      721 attgatcagt tggcctagtt ccccatgata gctgtctctt tgaatatacc catcttgcag
      781 cggtgaaatg aagtccccag cttcttttag aacaccgtcc atggtatcaa aaataacaac
      841 atctgcggct ttgattattt ctcgtggaat ttcacacatt tccggtgtgt aagcaccgac
      901 tccattgatg tgcgcacctt ttttgaccca ctcagcagag aatgtcgcac gttttgaagt
      961 ggtcacactt gtaatgatgt ctgctccttc aacgcattct tgactggttt ttgttgggcg
     1021 catcgttaca gaaaattgtt gcatcatttt ttcagcaaat tggctggcac gctcaaaatc
     1081 aatatcaaaa atccgaactt cttctaattt acgaacggtc aacattgctt ctaattgtga
     1141 ctgtgcttgt ccgccggttc caatcagcgc gccaatttta gcatcttctc ttgcaagaag
     1201 ttctgttgct gctccttgaa cagcgcctgt tcttaattgt gtcaaataag taccatccaa
     1261 acaagcggat accatacccg tttctggatc caatacgatc atcgttgctg gtacacttgg
     1321 taagtttttc ttgatgtttt caggatacac ggaaacgatt ttgacaccta gcgcgccttc
     1381 gccaccttct acataagcag gcatatataa gctttgtccg ttgctttttg gcacatcgat
     1441 attggtgcga agaggcacac ttgcctttcc tgctgaatac aatgacagtg cttttttgtc
     1501 cgcatcgatg gcttctctca ttgaaaaact tttgataata tcttcttttt taagaaataa
     1561 catttgaatc gctccttaat tagctgctgt aacttttttt aattgactgc ccaacatcgt
     1621 atatgcactg tatagtaaga ccaaaatcac gatccatttc aggatatagg tatctaaaga
     1681 tttgactagg aatacagcga ccaatacccc caatacaccg aaggttgaag taaaaagtgt
     1741 gatttttctg ctgtattcat taaatttaat gaattgaaca ctgccgatcg gtactgagaa
     1801 ggtacaagcc cccatcataa taggaaaggc tgcaactgga ttcaagccta gcaagtagac
     1861 agttaccatc gttaacgcat aagaaccaat gccgatattg ttcaatgcac catagacaaa
     1921 gagtaaaaat cctgccaaga ttaattttcc gccatacaat tcactggctg ttccgtttga
     1981 tggaatccag ttcatttgtc ctgcaaaaat caacaatgca gcaatgacca atcctatgcc
     2041 gacaaacagt ttgatcgttt tctctggtaa cttcacgaca aatcttggtc ctaaatatgc
     2101 accgataatt tggcagataa tacaaacagc tagtgtcttg atccctacgt tgatggcagt
     2161 gatgtaagac aatgccatga cggctaccgg tactacacat tgggtattca acgttccggg
     2221 tagttttttc atgctgaccc atttcaactt tggataaagg acggttccga tagcaaagtc
     2281 ggaaacgcca aatgttgaaa gaaagaagat gataaatgac gtgaagggca acagacgcat
     2341 atcccctggt tcttctttga aattttgttt gtttttgaaa aggtctctta gaaagtaaat
     2401 aataaaatat agattgattg cgacaattag tgtcagcaaa atattccgaa tcataaaaaa
     2461 ttcctcctac tagtaaatct ttctgattcc ctcaaagccc tcaccatatt ttgctttaag
     2521 ttcttcagga gattctgcaa tcacttttag tgtatatgca tcatgtttga catattcttt
     2581 tgctagattg accatggcgt ggttttttgt ccaagtgaca tcggttgatt gcagttcttc
     2641 gttaatggta ccaaacacga cttcttgctc atcggcgacg atgttgatcc atcttgctcc
     2701 aaagtcagca agtttgtctg tttcaaaacc atgaatataa tacttttcta agggaagatc
     2761 atattcatgg gtagaactga ataaaatcag tacaaactta tcaactcttt tttcagcttg
     2821 ctgcaatgcc tttagtagtg aatccgtgaa attctcatgc caaacttgaa tgagcaaact
     2881 ttcttttgcg tttttcacca aatgctctac tttatccaga acatactcga taccatcaac
     2941 tttccacagc atttcttctt catctttttc tttgatcatc cctagattct tggtaagaaa
     3001 agaaagatca tttttaacac ttttttctaa tttctctaaa aactcatttg ccgagatggc
     3061 atgataaagt ttaggttctg atttattaac gacaatcaaa ttctttttta atagtttttc
     3121 caaaatatta tatactttcg agcgtggcac acctgagatt ttacttactt catatcctgt
     3181 aagattacct ttttctaata gtgtggtata cactcttgtc tccatctctg aaaaatcata
     3241 ttttttcatg atttgaataa tcgtttccat tgattaatcc tcactttatt tttagtagct
     3301 actacggtaa caactataaa ttctttttga ttatctgtca agagttagtt caataacaca
     3361 catataataa ttagcttaaa aacaatcttt ttattaaaaa gaaacattat aatatgaatg
     3421 tttgtgaaaa aataatacaa cttagtcatt ctttacaacg atttatagaa aaacaacctc
     3481 tttaaatata aaaggtacgg gttactgatt acttgtaaat ctttacgttc cagttttatt
     3541 ctttctaaaa aatatactca cttcttttct tagacttctt tttttgattc tcctctttat
     3601 ggtcttgtcc ttttttgcct tcaaaatatt taaataaacc cctttcaaaa gcatttctag
     3661 gttttggaca ataaggaatg acttttttct tcatataaat acctcattta actatttgat
     3721 atcatcacat tatcaaaaaa aataggtttt ttattaaaac tatcttgacc tttcgggaga
     3781 tttcactgtg atttattttt attcccatac ttttttcgtt ggtcactttc gtatacctaa
     3841 tctaagcgat catcggcgac ggtgctttta caacgaatgg caggacaaaa caaggggcgg
     3901 aaaaactttt ttgcctattt cataaaaaac tttttcctac agcaatccct tgttttgtcc
     3961 taccacacgt tgtgaaccca aagcacgccg acgaacgctt agctatacga aaatgacctc
     4021 caaaaacaaa aaagtgaggt gaatagaaac aaaaagaaat tgaaatctcc caaaatgtcg
     4081 tccgcctcgg agacaacttg ctgctgaaaa tggttggtta attcatttaa gtattaaata
     4141 agataaatca tcaaattaat cggaaaaata gcatgattgg ccttatcaaa aataaactgt
     4201 aaaagcttat atttctatga gtggtggaac aatgctaata ccaaattaat ttatcgatta
     4261 cccacctgca caaaccaaag atagcattat taggaaacga atcaatcagc gaatccagca
     4321 ttataatgag gatactaaaa ataaagtttt cataattata aatccgcctt cctacgaaag
     4381 gcaccaatga ggtgatgaaa accttagagc gctttctttc tttttttatt ccgtgttacc
     4441 ctttccttgc aggtccctgg tacactacag aacccgagga ggtgagtggc ggggctgtat
     4501 agcggcaacc ccgcataatt atatggtgtc ggtcatccgt taaggcatca atgaatggcg
     4561 caaacccgta gcccgtaaac ttatctcttc cgaagttgac tcgatttcgc cggatgacca
     4621 gtccctacca aacctgtact atttttttac aggaggttgc tatgttcaaa atctttcgta
     4681 aaaattgttg tggcttggat gtccacaaaa cgtggatcta tgcgtgtatc ggaatcacag
     4741 atgcgaatgg tcgtacagtg tataagcaag ctcgcttctc ttcctttaca aaaggtctca
     4801 atgaattagc tgaatggctt gctaaataca actgtaagga tgtgtgtttg gaatcttccg
     4861 gtaaattttg gattcctgtt tttaatattc ttgaaaaaac ttgttttgtc acactggctc
     4921 atccgaagta caccaaaccc cagaaaggaa ataaaacaga tcgcaaagat gcaaaaatgg
     4981 atttgtgatc tctttatgtg cgacatgatc aaacccagtt ttattcctgg tcttgaaatc
     5041 cgccagcttc gtgatctaat gcgttatcgg acgaaactga ccaacatgaa aacaagtgaa
     5101 aagaatcgtg ctttaaactg cttaaccgtt tctaatctga aactagatga tgtttttagt
     5161 gatgtgttcg gaaaatcatc cacttctatc atccaatact tgttagaaca tcccggtgaa
     5221 tcatttgatg ttcgaccatt tatccatgcc cgttgtaaaa ctcccgttga agaaatccaa
     5281 gcggcagttg agggggcgat ttctgtacct caagcgatta agcttcgcca atgcctcaac
     5341 catattaacg agattgagaa gcatcgagaa gagatcaatc tagagattca agctcttgca
     5401 gaacccttct cccctgtttt ggaactactt cgtaccatac cgggcttaga cactcagcca
     5461 ataacagcga ttgcgattct ttcagaaata ggaccggata tgtccgtgtt tccaacttcg
     5521 aaacacttta tttcttgggc tggatgctgt ccaagaaacg atcgcagtgc ccgacgaaca
     5581 aaatcaacac gtatctctcg tgcagggcag tcgttgaaac cgctacttgt acagattgcc
     5641 aatgctcttc tcagatccaa agaacatcca gaatttaaaa atcgctatcg tagaatcaaa
     5701 gcgaatcgtg gacacaagaa agctatcatt gctatttgta agatgctcct gaccgctatc
     5761 tggaatgtct tatcaaaggt ggaaccctat tctgctaaag gatatctgga gccaaaacct
     5821 gcgaaagaag agaaagttct gacgcaatcc caagctctgg accttttaag aaaacgcgga
     5881 tataccatta tagatagtgc ttaaaccaaa gttacgttga ttcttcctaa atagactcgt
     5941 tgtcttccat ttcaaaaatt ttgaaaacgg taggcttatt tagtgtaccg ttcttcaggt
     6001 atcttcaaat agatttattt tcaaacttat ttcctttctt cttttactct cctatagctt
     6061 agcatttaag tgggcactcg tgtcaccttg ctagatagct atgaatgccg taataagcct
     6121 cctagtatat aggcagactg ccaattataa ctgattcaat ccacattatg ataaaatcca
     6181 ccttttcgtt tcatatgtgt tgaaatatca catatgtcaa aagtgcacga gtcttttcat
     6241 tcagtgcttt gtaaatactc aaaattattc cttttcactt taaaaaaaag ataattagta
     6301 caaaatcaga aatgaggaaa gtattaggat ggactagaaa ggagttgttg aatttgttgc
     6361 aacagattgg tcatttatca attaaaaatt aggtgaagtt ttattcatca ctgcagctgc
     6421 tgtttcccag agaataaaac gtctagaaga aactggtcag ttactgtatt atcaagcagc
     6481 aattgactac gaacaagctt gattactaac caaagcgttt atccacctgt tgattaaatc
     6541 aaatcaaaaa tcaggttttt atacgttcat aaaagcaatc tccagtgtac tcgaatgtga
     6601 ctgtatcata ggtccttatt cgatgttaat caaaatgggg tttccttcta ccgaatcatt
     6661 ggacagtttt atcaatgatt tatcaaagtt tggacgaacc aacacccaaa tcgttttctc
     6721 gactcttgtt tccttaatgg tttatcagtt ctggaatgtt tatcatgcgc ttttattaaa
     6781 atagaaaaga gcttttttca tgcgacaaag cagtgatcat ttattggcac atgcaatctt
     6841 tgtggatcca ttttttgaaa atacgatatt tagttttgac ccaaaaatct ttaaatacgt
     6901 atttgataat ttatcttaac aaccagataa ttttattcag caccgtatta tctatattga
     6961 attctcggaa tcaagataaa tttttttccg tttttgtttc ttttttaatc ttcttcttgt
     7021 tattaatagt gattaggatg atattcctat attgatcttt gataattaga tataattttt
     7081 gaataacgtt ccctgctatt cgagctagaa atacaagtac gccatgacct agttagcgac
     7141 aactacttag agatttcttt tgttctggga caacaaaatg ccatgacaat agcagtaaat
     7201 aatgatactt ccgtaaggct cgccgtaaac tggggagagg tgaaattcct atgtgcttac
     7261 ccaaagcggt tcaaaaattt taaactatta ttttaggaga tgattttata aaatcgaaag
     7321 cctacttgaa taaaaaacaa gcctgtgaat ttctaggaat tgataataat actttagata
     7381 agtggattcg tgtatataat tttccagtaa taaaaattga tg
//
LOCUS       DAYYKN010000131         7372 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000131 DAYYKN010000000
VERSION     DAYYKN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.133"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..299
                     /locus_tag="ACHBB7_002404"
     CDS             <1..299
                     /locus_tag="ACHBB7_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM9210307.1"
                     /translation="VGNNDYSQFYDWYKKFKYAIKSAEKEQKIQLKIIYEICNNKYFV
                     DYVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            375..593
                     /locus_tag="ACHBB7_002405"
     CDS             375..593
                     /locus_tag="ACHBB7_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210308.1"
                     /translation="MIENRLIDQVWCLDQNTDNLELIETICFNTIVLDLRSENDNCVT
                     HIIIDRKTAQKLSESLRKWAEGGNNENS"
     gene            580..3273
                     /locus_tag="ACHBB7_002406"
     CDS             580..3273
                     /locus_tag="ACHBB7_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM9210309.1"
                     /translation="MKIVKEKIASRKIHWLALSILVLMFIVVFTGNVVQASIFNWFDD
                     TESQTEFLNNPVYQSYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFIESTGMNKVYQSVLSTVVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     TIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISANIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNNGGNDQAEGEERKPERNFGQSLSKKANKTGKALGYAHERGLSGLASDGSTMAS
                     ERATKPFKSMRDIANDTKSKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPT
                     RLSDRKDGAKVENADSIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAK
                     NSNTGLEKLVKENPQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQG
                     TKTIDVRENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDV
                     RENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIID
                     SEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDSLLKK"
     gene            3287..3484
                     /locus_tag="ACHBB7_002407"
     CDS             3287..3484
                     /locus_tag="ACHBB7_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210310.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     KMKNEIESDFKCCSLEKNVRG"
     gene            3488..4099
                     /locus_tag="ACHBB7_002408"
     CDS             3488..4099
                     /locus_tag="ACHBB7_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM9210311.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4084..5475
                     /locus_tag="ACHBB7_002409"
     CDS             4084..5475
                     /locus_tag="ACHBB7_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9210312.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5691..6005
                     /locus_tag="ACHBB7_002410"
     CDS             5691..6005
                     /locus_tag="ACHBB7_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210313.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDSESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            5998..6492
                     /locus_tag="ACHBB7_002411"
     CDS             5998..6492
                     /locus_tag="ACHBB7_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210314.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTIDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESDKEETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFTL"
     gene            6569..6883
                     /locus_tag="ACHBB7_002412"
     CDS             6569..6883
                     /locus_tag="ACHBB7_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210315.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDRIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSETFEIDPDYYQPQEVFREFRNQIIRSSNI
                     EN"
     gene            7131..>7372
                     /locus_tag="ACHBB7_002413"
     CDS             7131..>7372
                     /locus_tag="ACHBB7_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210316.1"
                     /translation="MRSDSRFFVSNLQDDELIRQIDSLLETITESDKRIFLNYVELTR
                     HIIELDKLFNVFRYNLTNLLKHFTIFTNDLIESTGE"
BASE COUNT         2803 a          976 c         1427 g         2166 t
ORIGIN      
        1 tagtgggtaa taacgattac agtcaatttt atgactggta taaaaaattc aaatatgcga
       61 ttaaatctgc tgaaaaagaa caaaaaattc aactgaaaat tatctatgaa atctgtaata
      121 acaagtattt tgtagattat gttagagaac agctatcaat gactttacgt gacttgatac
      181 gaagagcaaa aacagatcac cgtatcaaac aaaaagacga ttatatcttt gcttctctaa
      241 aaaataaagc gttagaacta attgaattgc aaatcagtga aggaatagat aagcaataag
      301 aatagaattg agtcaaatga agtggagggg tatctccgct tcatttgaga actttgaata
      361 taggaggggt tattttgatt gaaaatcgtt taatagatca agtttggtgt ctagatcaaa
      421 atacagataa tctcgaatta atcgagacta tttgcttcaa cactatagtt ctcgatttaa
      481 gatctgaaaa tgacaattgt gtaactcata tcataattga cagaaaaaca gctcagaagc
      541 tctctgagag tctcagaaaa tgggctgagg gaggtaataa tgaaaatagt taaagaaaag
      601 attgccagta gaaaaataca ctggttagct ttgagcatac ttgttttaat gtttatcgtt
      661 gttttcaccg gtaatgttgt ccaagcaagc attttcaatt ggtttgatga cacggaaagt
      721 caaactgaat ttttgaataa tcctgtctat caatcgtatt taaaaaaata taacggaaat
      781 ttttacagtc agtttggatt gtggataggg tggtctgtta taaaatcgat gtttgcaatt
      841 gtagatggta ttcaaaatat gattcccgaa gtgttagatt tgtttagctt tattgaaagt
      901 acgggaatga ataaagttta tcaatcggtc ttaagtacag ttgttattgg tttaatgata
      961 ctttcactga tgtttgttgg ttacaaaatg attactggaa aaggaacagt ggatttaaaa
     1021 tcggttggaa tgaacgtagt gatgtctgta gcattaattc ttttaatgcc gactttgatt
     1081 ggatcaacag aaaaactttc aggaataatg tttgcaaaga ctctttataa tgacgcaact
     1141 agtatcaata atcaagatgg cggtgtagca tggtcattga ttgagcaggg agtgacagat
     1201 ttagcttata ttaataagac agatcaatat tcgacaattg ataaaacaaa tgaccgaaat
     1261 aatctaacaa aagaaaattt ctggaaaacg gatcttacgc aagtattgac agataatgta
     1321 attgataaaa tggaaaaaga gaacccaaca gctgataatc tgcgttatga attagttgaa
     1381 aattctgata atgaatttgt tgctactaaa ttttcagata attttttatc aacctttgtt
     1441 gatggtatca aatcaggata ttatcgctat caagcgaacc tgtggggaat ttcttttgga
     1501 ttgattgctt tagcggtagc gtatgttttt agtgctttca ttataattac agcaatactc
     1561 gagttagcat ttaaacgtgt actcggagtt ttagtatttg ctactgactt agaaacagga
     1621 caacgatcaa aacaagtttt gtcagatatt ttacagtgct atttgacagt cggatttcaa
     1681 gggttcggtt tatctatgtt tgcaatgttc ataaattacc taaatactgg cgaaggtatc
     1741 tcagcaaata tttttataaa aacaattgct tatatctgtg ctgtgtttgt tttaatcaaa
     1801 ggcagtggaa cagtcatgag atactttggt gtggatattg gactcaaaga aggctatggg
     1861 caacttgctt ctgcttttgg aatgggtgca atgctattcc gtaaaggaac aggtggtatg
     1921 aaccggtcta aaggtgggaa aaataatggt ggaaacgatc aagctgaagg ggaagaacga
     1981 aaacctgaac gcaactttgg acaatcactt tctaaaaaag ctaataaaac ggggaaagcg
     2041 cttgggtatg ctcatgaacg tggattatca ggtttagctt cagatggatc tacaatggct
     2101 agtgaaagag caacaaaacc atttaagagt atgcgtgata tagctaatga taccaagagt
     2161 aagtttaaag aaggtttaga tgatggtact gtgtccgcta ttaataaaaa tagcaaacct
     2221 atgttagcta aaaataaaga agatgaaact ggaaaatatg ctgattccat gccaacacgt
     2281 ctttcggata gaaaagatgg tgctaaggtt gaaaatgccg atagtattat gtctagttct
     2341 gaacgtatgc gtgaagcaat gaaaaataat gctgaatcga ataataatcc tgtaagtgca
     2401 attcagcaaa aagtccaaca agatattgaa gaacgtaaaa atgctatgca cggtcaagct
     2461 aagtctgctg aagaattaat taatcagaag cgtcatgaag cgaaatacac gcctgaagca
     2521 atgaatcgcg aggaaataat gttaaggaaa cgtgtagaag ggcggacagg cgctaatatt
     2581 gacggaaaag aagcgttgac caaagaaaga attcaagaag ctaaaaattc aaatacagga
     2641 ttagaaaaat tagtcaaaga aaatccacaa aattctacaa gtagtcatgg tggccgatca
     2701 gtagatgtcc gagaaaatat tcaaggttca ctgaatggtc atgttggacg gaatgttgat
     2761 gtaagagaga atttacaaag ttcttcaaat gctcaaggta ctaagacaat tgatgttcga
     2821 gaaaatctgc aaagtagcag tacaagtcaa ccaaaaacag tagatgttcg agaaaatgta
     2881 caaaattcta caagtagtca agggtttaaa tcagtggatg tccgagagaa tgtgcaaggt
     2941 agcagtgcaa gtcaaccaaa aacagtagat gttcgagaaa atgtacaaag ttctacaagt
     3001 agtcaaggat tcaaaacagt agatgttcgg gagaacgttc aaaaagatag tggagaaatg
     3061 aaagaaaaaa cacaaaaaat caatatcgtt gaagacagaa agtcagcgaa aaaatttgat
     3121 tcaaatcatg aaacagtgat tattgatagt gaaatccgtg aaaatgataa gggatcaaaa
     3181 ccaagacgaa ggtttaccta tgaagacaac gagttattca gagatacact taatgatcct
     3241 aatccacttt tcgatagttt acttaaaaaa taaagaggtg attgtcatgc aattaattct
     3301 acagattagt acacatgatt tcttagaact tgacgaatta tgtgaatcag taaagaaagc
     3361 aacaaatgaa aaaatcacgg tcactaatta tattgaactg atgattcaag aagaattaaa
     3421 gatgaaaaat gaaatagaga gcgatttcaa atgttgctct ctagaaaaaa atgtgagggg
     3481 ataatcaatg gatgataaac aaaaaatgta cgaaaaaaat cgcaaacata gtgaggcctt
     3541 aaattttcgt gtcgataaaa agacttatct tgaactgaca ttgattgcaa aacaaagggg
     3601 aatttctaga gcagacgttt gccgtgagcg gttaaaggga ctcaaatatg aaccgccgtt
     3661 gttagctgaa gatgataggg tagttttaac caaacagtta aatcaaattg gtaacaattt
     3721 gaatcaagcg gtaaaggcat taaatatcat tgctaaatat tttagagaag aatctgaaga
     3781 taaaaaagaa aagagcctac attttagttt tgatgctagc cgttacaaca tttttaaaga
     3841 tgaacttgcg aatagcatag caaaaaagtt gatgattagt tatccagatg acattttttt
     3901 ggagtccaat cggagtgccc cctctttttt taaattatca gaagtagaaa aagaatattt
     3961 cgaatattgg atagaaaagc aaactagacc gggtacgaaa gaagagtatt cccaaaaagt
     4021 tgatcggaat ttaattcact caattcagat tattgcaagt ttaaaagatg aggtgaaaca
     4081 actttgggag ctactataac atcgaaagga cgcaagaaaa atttatcctc gctattacaa
     4141 tatgcagcta atccttcaaa aaaagaaaaa tgtgttgcag tctcaggaat aaattgtagt
     4201 gatgatagtg aggaagcact gcaagaaatg aatttagtac gtaccaagat gaataaatct
     4261 aaagatatag cacaagcgtt tcatgttatt cattcgtttg ataagagtac ttctaaagag
     4321 ttaagtattg ataaaatgca cgaaatagct gtcgaatttg cagagaaagc ttttccaaat
     4381 tcgcaaatca ttgttgcaag tcacaatgat aaagatcatt ttcatagtca tcttgtcatt
     4441 aataatatta acatggaaac atgtaaaagg ttgcgtattg accctaaaga tttagaacta
     4501 atgcgaaaaa ttaacgatca agtcctcaat gaacatggtc ttgaatcagt gaataatgag
     4561 tactatgaaa acttagaaaa gagaaccaat ctttattcaa acgatccttc taagatgaaa
     4621 tatggaaaaa ttaattatca agagtttgtg caaaatgcga tcgaagcaac gttggaaaaa
     4681 tgcgaagtga attcttttga agatttttcg gatcatttgg caaaagaata taacattgaa
     4741 gtttatcaat tttcaaaaac aaataacaaa cttggctatg ttctttacaa acaaggtgta
     4801 aatttctatg agcgtagagg tgagttaatt actttaggaa gagataaaca atctagaaaa
     4861 gaaaaaagtc agtatgtcga acgtactttt agtgcaagaa agttagggaa aagatattca
     4921 tttgaatctg tttctgaaca acttgaaaag aatttagaag aatcacatat aaaaaatttt
     4981 tttaatatgg aaagagagca agttatgtcg ctaattagtg gagtaaatta caaaactggt
     5041 gagttaaatg taccttttgt ggaattagat tccatcgcac aaaagcaatt tagcgaccgt
     5101 gaaggcgatg aaaaattcat taaatatgat gacttcttgc gagaggtaga caagcatttt
     5161 gtaaacacaa tgtctttaga agatgatcaa gaatacacga ttaaacttca tacggatgtg
     5221 aatcctcaaa ctttacgtag tgcgaaaagt aaaacaattt ctattaaaaa gactgatcag
     5281 aaagagttat ttctaccggc aaagggtttt gaaaattgtt ctcatacacc ttctgctaaa
     5341 cagattcact atcaaatcag tcgcataatg aatgacgatg taaaaatgta tcgtgctaaa
     5401 aaggattatg aattcacaca acaaatgatt gaaaatcagc aaaaaaggca acaaaatttt
     5461 gaaatggaat tctgaatggg cttgttagtg gactttccag ttttttgcct atgaagagtt
     5521 gcttggaaag aagcagtttt taggacatac gccagctacc catctacttg ccagtcaatc
     5581 cgccccgctc acgctaggct actttctggc aagtcagatg gtgggtaaat ctctgaggga
     5641 gcaagctccc tacagactcc ctcccaaaaa aagaaaggtg gaagaaaaaa atgaactatc
     5701 catattttaa agtctcggct tcagaagaaa ctaaagaaat attcaacaat ttttacaacc
     5761 aaaacaaagg agtttttggt tccaaggcaa atatgtttcg tgttatggtt tcaaatttac
     5821 cagtgttagc tagtccatca aataataaat ttaatgattc tgaaagcatt aaatttgaac
     5881 aaaaaatttc agaactagaa tcaatgataa gtaatgaagt aattgaaaag cttgatgaca
     5941 ttgatcaaaa gttatcgtat tctctaaaaa acaaatacaa aacggaggag aaaaaagatg
     6001 tttaaagttt acttcccgtt tcagtggctc gttaaaggaa ttcttttagt gctactagga
     6061 ttgattcttt ttggaagtta tctaacaatt aaagccagac attttccaga agaaaagcca
     6121 ataaaaattg taacaataga tttaaaaata ggtgaaactt atcaggcaga tggggttgaa
     6181 ctaacattta aaaatattga agtgaccgaa aaaagaaatg aatttgcaca acatgatcgt
     6241 gtattacaag tgacctatac acttaaaaat gatacagaaa aaccaatacg tccacaacaa
     6301 tggtcgattg aaaataagga tggtgaagag ctttatgagt atgctatgat attagataat
     6361 ccagagtata ttgaaagtga caaagaagaa acagtaaatt actattatga attaccgatt
     6421 gaaaggcagg actaccaaat cgtagtacgt agagcgaatg gttattttgt tggattaccc
     6481 tttactttgt aagtcttatt ttgttagtgg cttttaagtt aatatcattt ttgtgatttt
     6541 tgaaaatgaa agtgaggaaa ttggaagaat ggctgaagaa aataaagttg ttccgtttga
     6601 aacattagtg ataaataagg aagagtttga gcaacttttt cttgattttt ttattcaata
     6661 tgacaggatt tatgatttta gttcgtcact agatttattt gaattagaag cagaaataat
     6721 agaaaacagt cgagagaaga atggtaaaat cattatttca gtagaaaaat tcaatgaatt
     6781 attctcagaa acatttgaaa tagatccaga ctattatcaa ccacaagaag tttttcgaga
     6841 gtttagaaat cagattattc gttcaagtaa tattgaaaat tgagtcaata aaagaagcgt
     6901 tgaagaaaag ttccttcttt cgacgcttct ttttaaaaga ttttacaaat gtcttatgga
     6961 gatttgcttc aatgactact tgtagtcatt tcgtttaaaa taattatgtt acacccttat
     7021 gtgtcacaga gaaatactga tataataaga aataaggtat gtaacaatag agtatacttc
     7081 tatgttacat attgattatt ttgaaaaaat catattagga gaatataatt atgagaagtg
     7141 atagtaggtt ttttgtcagt aatttacaag atgacgagct aataaggcag attgattcat
     7201 tattagaaac aataacagaa tctgataaaa gaatattttt aaattatgta gaattaacac
     7261 gtcatattat agagttggat aaacttttta atgtgtttag atataattta actaatttgc
     7321 tcaaacactt cacaattttt actaatgatt taattgaaag tacaggtgaa aa
//
LOCUS       DAYYKN010000132         7369 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000132 DAYYKN010000000
VERSION     DAYYKN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.134"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1259)
                     /locus_tag="ACHBB7_002414"
     CDS             complement(27..1259)
                     /locus_tag="ACHBB7_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM9210317.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="ACHBB7_002415"
     CDS             1514..2083
                     /locus_tag="ACHBB7_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9210318.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="ACHBB7_002416"
     CDS             complement(2261..2701)
                     /locus_tag="ACHBB7_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM9210319.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="ACHBB7_002417"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="ACHBB7_002417"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM9210320.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..4578
                     /locus_tag="ACHBB7_002418"
     CDS             3655..4578
                     /locus_tag="ACHBB7_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM9210321.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4803..5042)
                     /locus_tag="ACHBB7_002419"
     CDS             complement(4803..5042)
                     /locus_tag="ACHBB7_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210322.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5047..5466)
                     /locus_tag="ACHBB7_002420"
     CDS             complement(5047..5466)
                     /locus_tag="ACHBB7_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM9210323.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACHBB7_002421"
                     /gene_synonym="mefH"
     CDS             complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACHBB7_002421"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM9210324.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7038..>7369)
                     /locus_tag="ACHBB7_002422"
     CDS             complement(7038..>7369)
                     /locus_tag="ACHBB7_002422"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210325.1"
                     /translation="LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSL
                     EIENSHEIAEVIMVLSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETIS
                     AFIQFCK"
BASE COUNT         2373 a         1364 c         1293 g         2339 t
ORIGIN      
        1 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
       61 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
      121 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
      181 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
      241 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
      301 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
      361 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
      421 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
      481 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
      541 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
      601 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
      661 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
      721 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
      781 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
      841 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
      901 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
      961 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     1021 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     1081 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     1141 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     1201 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     1261 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     1321 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     1381 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     1441 tcaaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     4561 tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttattt
     4621 gaaattttat atctaatttt caattatccg cataaacact atacattcaa gcattttgaa
     4681 gtatagaaaa acactcatct taccacgaat ttaccacgat tgaatgagcc ttgatatgac
     4741 cgtaaaacta ctaagggaga tagcacgaaa ctgtgtatct cccgattttt ttatacagtt
     4801 gattacttca ctccgatttc aaatttcaat attgctttca tcatgtctgt atgaatttct
     4861 ccttttagtt cttcatcaac ctgcataatg attttaccac tttcagagtg gtaaaaagga
     4921 cgcaagcata atttagaaat ataaccgtca taaaacttca aaatctcttt aactgccatt
     4981 tcatctccgc ttgacgcttt gcaaatcaat tcaaaagagg gatagccata atctttactc
     5041 atttctttac ttcctttcgc tcaaaagcat ttttaacttc cttagtcctc tagttcttct
     5101 actgcaaata gcagaacgtg atacatggta cagttctgaa atttctctgt cgttcattcc
     5161 ttgaaaataa cgtaataaaa tcgcactacg ctgtttttct gacagtctat gtaaaactgt
     5221 accaagtgtt tcatcagata cataaattgt aaagtgcaag actttaaatg atacacaatc
     5281 ataaaaacag gtatcaatcg ttccacgttc aacttgtttc gtttcggcta tttcacaaaa
     5341 caatacttca cgtttagaat gtcttgcaag ctgtctgttt cgacttttca ctttgttctt
     5401 aatgacaatc agcattaaag aattgaactg taatctgacg atatgctcga aagaagatgt
     5461 cctcataatc tcaccccctt tctgctaatg ctgaaatata taaacggaat accgtttata
     5521 ccctttagaa atacagaaaa atggtaagaa aaaagcccac acaaaacata tatttgtgcg
     5581 agccttaaat actgcaatat tactctttct tttataacac tatcctttta gcagatttca
     5641 aattttcttt tatatattgt ttttagccta acatactatt taaccccttg tattatatct
     5701 ctaacttttg aaaaattctt tttgaatata ttgaaatcat gattgaagca atagaagcta
     5761 caaacataac aacccatgtt tcttgtataa atacatcgaa taaaataccg taaaccattt
     5821 gtccaatagg ttgaccgcac atagcaactg ctaccagcgt agccattatc tttcctacta
     5881 attgcggtgg tgtttgtgtc tgaacaagag tgtaaatttg aacaacaaac atagtagata
     5941 cacccatagc cacaaagcac atcactgtaa ctacccagta ggaaactatt gcaggcaatt
     6001 ctaaaaatag cgaaattccc ataagtaaaa cagaaacaga acaaataagt aacaaggtat
     6061 aaaggttttt tagttttaac tttgaagcaa caagagctgt taaaatacca ccacataaac
     6121 cacctaatga taatgctgat tgtgaaaaac catatagtgt atctggcatt tttaaaactt
     6181 cagaaattaa gataggcaga ccaacaatta aaacagcact taaaaccaaa ttgaaaatgc
     6241 aaaccaaaaa gattacggat ataaaaatgg gtttctcctt tttcaaaaag gtataactat
     6301 cttttaaatc gtttttagca actgcaatta cacctacttc acgaggtctt ttttggtgtg
     6361 gaatatggat aaaaatttcc ataatggctg aaaaagtaaa acatataata ctaacaatta
     6421 aaatgggatt gataccataa aattgaaaaa ctactccgct tatagctggt ccgataaagt
     6481 ttgctaaagt atttacttga tttattatag cattaccgac ggtaagttta tctgatgata
     6541 ccaatagagg aatacttgct tgtaccgctg gttgatatgc accagaaata ccataaagta
     6601 acatcaaagc aacaataaac aaaggtatga taggaatttt tcccaatgac aaataaaata
     6661 ctaaaattat gattgctgtt acaaaatcta gtatgaccat aatgtttctt ttatttgtcc
     6721 tgtcagcaat aataccacct accagcgaca aaataaccat aggtaaaaaa gagcaggctg
     6781 tagcaatacc aaacaaagct gatgaaccag tttcttttaa caaatagtac ggtaatgcaa
     6841 atcgtaggat agcgtttcca aataaagaaa ttatctgtcc tataacaaca agtataaaat
     6901 cacgattaaa aagttttttt tctgcttgca taaaatctct cctttttatt taatcaaaca
     6961 gaccgtcggt ttatataatg gtttaaataa actgctcttt tatagagcag tctatcgtat
     7021 agggataaca aatacagtta tttacaaaac tgtataaatg cagaaattgt ttcttcgtct
     7081 aataatgtgt ctatgcctat tccatttaaa agggcattga atgtattgca acgtttttcc
     7141 ataccctctt tatccgtcat ttcaactaat gggttgagcc atacattact taataccatg
     7201 ataacttctg caatttcatg tgaattttca atctccaaag acttatcctt gataccttgt
     7261 tctaaaacgg gttcaacaaa ttcaggtact acaatttcat acatttgttc tatttgcata
     7321 gcaagaaaac gaggattgtt taatagttga ggagaaactg tcaataaaa
//
LOCUS       DAYYKN010000133         7330 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000133 DAYYKN010000000
VERSION     DAYYKN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.135"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..939)
                     /locus_tag="ACHBB7_002423"
     CDS             complement(79..939)
                     /locus_tag="ACHBB7_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM9210326.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(998..1630)
                     /locus_tag="ACHBB7_002424"
                     /pseudo
     CDS             complement(998..1630)
                     /locus_tag="ACHBB7_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            1797..2558
                     /locus_tag="ACHBB7_002425"
     CDS             1797..2558
                     /locus_tag="ACHBB7_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9210327.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            2584..5175
                     /gene="mprF"
                     /locus_tag="ACHBB7_002426"
     CDS             2584..5175
                     /gene="mprF"
                     /locus_tag="ACHBB7_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM9210328.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(5363..6550)
                     /gene="tuf"
                     /locus_tag="ACHBB7_002427"
     CDS             complement(5363..6550)
                     /gene="tuf"
                     /locus_tag="ACHBB7_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM9210329.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            6918..7274
                     /locus_tag="ACHBB7_002428"
     CDS             6918..7274
                     /locus_tag="ACHBB7_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9210330.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         2162 a         1453 c         1327 g         2388 t
ORIGIN      
        1 acacgaatga ttttacaaaa aagatgccaa cctttttgtt tgaatcaccc gtgtgctgtt
       61 tttattatca atctatcttt acttatattg ttcctccaaa cggctcatcc accaacctag
      121 tgctgtaccg gctataaaaa gaacaggaca agctagtacc ccagcaactc ccaatcccgc
      181 gtaatcggta ggaatgatca taatcgttga agcaaaaact acacctaaaa tgaaatggaa
      241 tagcttgcta tatgcctttg agaaaatgta atccatgatt tttgacaatc caagaacaca
      301 aagaatccca ccaattccga tcggcacaat cacttcaagt cgtacttctt taaatccgtc
      361 agccatcgct ttatacaatc ccatatacac caaaaaatta gaagggctca atcctgggac
      421 gatcataccc aaaccaatca atccgccggc aatcagccat gttccaaaat tctgatcgac
      481 ttgcccgtca aataattgag cgccgtataa taaaaacaaa taagccagaa caaaagtaac
      541 aaccatgatg atcacatcag aagtatctct tccttttttc cctgcttctt tccataacgc
      601 aggaacagtt ccagcgatac atccgacaaa aaaccataat atcgttgttt catacgcacc
      661 taataaaaaa ctgactaaaa aagaaagtag aaagatgcct gtcactccgc ctagaccaac
      721 aggcagaaaa aacaaaacat tttctttgaa attttttgta aggtgggcta aaaaagaaat
      781 cagccgctca tacatgccaa agacagctgc taatgcacca ccgctgactc ctggcaagat
      841 aaatcctgaa ccgataaaca ttcccttcac aaaccgtaac atccagtctt tggttgtgta
      901 ccccacttct tgattttcca ttttttgcct tttttccact atttgcgtac tccttctttc
      961 atttaatttt attcatgatt gaacatttcc tgctatttta cttaaagact ttttcatagg
     1021 tctctattat tttttcattc attttgacaa tatcaggata aaacatgtta cctccattat
     1081 gatagagata gccgccattg taaagcaagg actggatccg ccaatatcgg tattgtttcc
     1141 cttcctcatt tcctaaaagt gggctcagca catctttaga atacgcttct gctaattcta
     1201 ctgtgttttc tccgccatga ttttttacat aggcaatata atctttccca aaattatatg
     1261 cttggacagc tgtccataaa tcgcatcctt gtttctttgc ttctactatc atttccgcca
     1321 aatatttgac accttgatca atactgtcag ctggattatc aatgatctca gcttctccat
     1381 gcagactttc cgagctctgc atgagatctg tcccttttcc ttttgattcg gtcaatatta
     1441 tcgccttgat caaattctcg tagttttcca tatgataatt ttttgcagct gttgttattt
     1501 ctatttgata gctttccact cgttttaccg aacgataagt tcttcctcat aaaaacaaca
     1561 ccgcaccaat aaccgtcaga atgatcatgt aaaaaagaat acggataata gataatcgtt
     1621 ttcgcttcat ttcatcacct agttattttg acatcttttt ttatcttact atttctttat
     1681 cttcttggct atttcttcct tgatttctgt gtacgcctta agaaaaaatg aattttcctg
     1741 tattttttga catcttacga aaatgatcgt tgtttttcag taaaaacaat tgcccaatga
     1801 ctgatataat cgttatactt tacacagtag gtgattatgt gaaaaaagaa aagaaatatg
     1861 caaaaatgcc agatgggagc gaaatttact atgaaaagag cggtcaaggc ttccctctct
     1921 ttttactgca tggcaatgac ggcagcggcc gctttttttc agaacaggtc cctgtactcg
     1981 aacgctacta taccgtgtat ctagtagata gccgaggaca tggtcgttcg acaaacgaag
     2041 catctatgtt gaattttcag ttaatggccg aggatctgaa tacgatcatg ttgctggaaa
     2101 aaatcgatca ggcagatttt cttggattta gtgatggagc aaatctagca ctggtatttg
     2161 ccagcagctt ccctaaaaaa gttcatcgat tgattttaaa ttctggaaac acgcttgtta
     2221 aaggcgttcg tttttctgcc agagtcatca gtaatattca ttatgcatgg gtatggttac
     2281 tctctttatt tcgcccaagt cttcgcaaaa atcttttagt tatcaaactt ttgctgcatg
     2341 acatcgggct tacagaaaat gatttaaaaa aaatcaactc ccccactttg atcatcgtgg
     2401 gaaaaaaaga tgtaatcaag ctaaaacatt ctttgtatat agctaaaact ataccaaaag
     2461 cttcctttgt gcttgtgaaa gaacaaggac atgaattagc tagaaaagat cctgaacgct
     2521 tcaatcgaga agttcttcaa tttctttctg aaacataact attaggaata gaaggtgatt
     2581 tatttgttaa aaaataccat acaatggctg aaaaaccacc taggactgtt caaaacaatc
     2641 tttttgattt ctgtcattgt cattattgtc ggtgaattga tgtccatcgg taaaactcta
     2701 tctatccagc aattaggaga aacgtttgcc actatacctg tatggaaaag cggactaatg
     2761 ctattgattg gtcttatctc tgtgttacct atggttggtt atgatattat tttgaatcgg
     2821 atgcttgaac aaaagcagaa tcctcgttac ctgtttgaga caagctggct gatcaatacg
     2881 atcaacaata ttgccggttt cggcgggttt gttagtgtgg ggcttcgctc tgaactatat
     2941 ggacaaaaga aagagagcag aaacgtgatc caagcattat cgaaagtttt cttgtttttg
     3001 atggctggac tttctattta cagtctgctt tcttttttac ttgtagtctt cacgccagtt
     3061 gctccttttt tgaagcaata ttggatatgg ttgatcggcg gcggtctata ttttcctgtc
     3121 gtctttttat ttactacttt caaaaagaaa ggattccttg gtggtacctc acttaaaact
     3181 caaggacagc tattgctagt ttcattgttg gaatggaccg gtgtcctgtt tagtttcctt
     3241 tctgttggtt atttaatgga aatccggatt gatctgtggc aaacaattcc attattcatt
     3301 gctgcttcag tgataggaat cgtgtctatg attccaggag aaattgggag tttcgatgta
     3361 atgatgatta tcgggttgtc cgctattggt gtgccaagag aaatagtcgt tgtgtggatc
     3421 ttgctctatc gcctgttcta ctacatcatt ccgtttttga ttggaatcgt ctttttcttc
     3481 aaaaatatcg gttccacttt tgatcagcgt tattctggta tccctaaaca attagcaaca
     3541 gaaattgcgc ataaaatcgt ggttgtctta ttatactttt ctggaatcat gctagtactt
     3601 tctgccacta tcccgcaagc ttttacagag tttcgctggc ttcatagttt gaacccattg
     3661 aaatttcatt ttattatcca atttcctagt atactattgg gatttctttt aatcattatg
     3721 ggcagaggga tcgcagcacg tgtgaaacga gcatatttgc ctacaatctt cttgattgtt
     3781 ctagctttac tttatgtttt attaagtgat tttagtttca caccggtggt tttcttgtct
     3841 attctgcttc tgatcattct tgcttctaaa aacgaattat ttcgggaaca attgatctat
     3901 tcatgggagt ggcgtacgat cgatggaatc attattggtg cactgtcctt gctttacatc
     3961 gttattgggg tatacaatct gccggatttc cctcatcggc gtcaccattt tatttccttt
     4021 ttcttatttc cttctgaaaa gatttggttt tcgggattgt tggccatcat cgctgttagt
     4081 ttcatgatcg ttctatttgt tcatttcctt caaggagaaa agaaacagat tggcgaagcc
     4141 tttaatgaag aaaaggccct gaagatcctt acaacttatg gaggaaactc cgatagtcaa
     4201 ttgatcttct taaaagataa gcggatgttt gcctatgaaa aagacggtga accaactgtt
     4261 cttctccaat tcgcttgctt caataataaa tgtattgtaa tgggtgatcc ttctggtaag
     4321 aaagaagatt ttccagaagc aatcgaagct tttatcgaag aaacagatcg actatgctat
     4381 ttaccagttt tctacgaaac aagcgaagag attgttatga ttttgcatga attcggctac
     4441 gatttcatta agatggggga agaagcttat gtagacctga atagttttac aacttcaggg
     4501 aaaaaaatga agggcactcg tgctgtcttg aatcggatcg aacgagaagg atttacattt
     4561 gatgtcttac aaccaccatt ttctactgag caaatgagta ctttcaaaaa tatctctgat
     4621 aattggttgg gttcacgaaa ggaaaaaggg ttttctttag gctttttctc tgaagattat
     4681 ctacaaagag cgcctattgc agttgtcaaa aatacccaag aagaaattgt cgcttttgca
     4741 acgatcatgc ctacttacac gaacaataaa gtaggaacga ttgatctgat gcgatatgat
     4801 tctaaaaaag caccttctgg cagtatggat ttcttgttct tgcatttgtt tgcctatatg
     4861 aaagaagaac atatccaatg gttcaattta gggatggccc ctctttcaaa tgtcggaact
     4921 tccagaaaga gttttttaca ggaaaggatt gcatacttgg tttacgagtt tggttctcat
     4981 ttttattctt tccatggatt aagagaatat aaaagcaagt acgctacaaa ctgggtctca
     5041 cgttatactc tatattctag agatagttgg attgcatatg ttatgattgc tttgctgatt
     5101 attgataatg cgccagttga gcagacacaa ggtagaataa gtggtttgaa aaaatggata
     5161 cgaagaaagt attaaaaact gcataccgat agatgaacac tctcatgatg aataaaagag
     5221 actgaagtaa aacgaaagtt catgcttcta caatctcttc ttatacaatt gaagaacaaa
     5281 gagcggtcca aacagaaatc ctgtttggac cgctctttta ttttaaacat ttttcattac
     5341 caaaatattc gagtcgcgtt tcttatgctt cgatctctgt aacgatacct gaacctactg
     5401 tccgtccgcc ttcacgaatt gagaatgtcg ttccgttttc aacagcgatt ggatggatca
     5461 agtcaacatc aatcgtcacg ttgtcaccag gcatgaccat ttctgtatct tctggcaatg
     5521 tgattgttcc tgtaacatca gttgtgcgga agtagaactg tggacgatag ttgttgaaga
     5581 atggagtatg acgtccacct tcttcttttg tcaacacgta cacttctgct ttgaattttg
     5641 tatgtggagt aatggaacct ggtttagcaa gtacttgtcc acgttcgata tcgtcacgtt
     5701 ggatcccccg taacaacacg cctacgttat ccccagcttc cccgtaatct aacgttttac
     5761 ggaacatttc tacacctgtt acaactgctt tttgtgtttc aggtttgatc cctacgatct
     5821 ctacctcatc accgacacga acagcaccac gatcaatacg acctgaggca acagttcctc
     5881 gacctgtaat tgagaagacg tcttccactg gcaatagtaa tggtttatct gtatcacgtt
     5941 ctggtgttgg gatatattca tctactgtat ccatcaattc cataatagca gcttcagcat
     6001 ctggatcgcc ttgcaatgct tttaatgcgg aacctttgat cacaggagtg tcatcgcctg
     6061 ggaaaccata ttcgctcaat aactcgcgaa cttccatttc taccaaatcg atcaattctt
     6121 catcatcgac taaatcaact ttgttcaaga aaacaatcag gtattttaca ccaacttggc
     6181 gtgacaataa aatgtgttca cgtgtttgag gcatcggacc gtcagttgca gaaacaacta
     6241 agattgcacc atccatttgt gctgcccctg taatcatgtt tttcacatag tccgcgtgtc
     6301 ctggtgcatc aatatgcgcg tagtggcgtt tttctgtttc atattctacg tgtgcggtat
     6361 tgatcgtaat cccacgttca cgttcttctg gagctgcatc aatactagca tagtcttgag
     6421 gatttgctag gccttttttg cctaatactg tagtgatcgc agcagttaaa gtagttttcc
     6481 catggtcaac gtgtccaatc gtaccaatgt taacgtgtgg tttacttctg tcataatgtt
     6541 cttttgccat aatttacaaa cctcctaaat aatatagatc actgaaaaat ttcagctctc
     6601 acctttttat tatagtcttt ttttgactaa atcaaaaaga atacgtccga cttacttttt
     6661 gtaagattcg aactaagctt attatacgtc aatattggtc aaagtcaact aagagcactc
     6721 gtctgtctta tacgcgaaaa aagtaccttt tataaataaa aaaatgtgtt ttttgagaaa
     6781 aacttcaaga tagtccttaa ctttcttttg aagcaataag aacaaaatga ggaaaagttt
     6841 aaaattcagc tgttgccaac aactttcaat agacgaaatc ttctatttcg ttaataataa
     6901 ggaaagaggt gaagtacatg aaaaaattaa ttggcgtact tgtcgtttta ggcatatttc
     6961 tcggcggaag ttttgcagct tatcattatt tttacggcgg cgaagcttat tatacaaaaa
     7021 tcacgacgaa tggcgaaatg tcagctaccc aagcagataa cggagagaaa ttcacgactt
     7081 atacctacaa gcaagcggct tataataaag atggtgaaaa aaaacaggtg acgcttcgcg
     7141 aagaaagaga acatccatta aagatgaatg cgtatcttaa actcaaagtc aatcctcgta
     7201 aaggtgtatt aagctgggaa gaaataaagg cttctgaagt gcctaaaaat gcggcagaaa
     7261 aaatcgataa ttagtaaatg gtcttaaaat tttattagat aaaaagaggc tgggacagaa
     7321 gcgtttaact
//
LOCUS       DAYYKN010000134         7327 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000134 DAYYKN010000000
VERSION     DAYYKN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.136"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(405..779)
                     /locus_tag="ACHBB7_002429"
     CDS             complement(405..779)
                     /locus_tag="ACHBB7_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9210331.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(802..2202)
                     /locus_tag="ACHBB7_002430"
     CDS             complement(802..2202)
                     /locus_tag="ACHBB7_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFM9210332.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2206..2616)
                     /locus_tag="ACHBB7_002431"
     CDS             complement(2206..2616)
                     /locus_tag="ACHBB7_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9210333.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2634..3125)
                     /locus_tag="ACHBB7_002432"
     CDS             complement(2634..3125)
                     /locus_tag="ACHBB7_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9210334.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3135..3926)
                     /locus_tag="ACHBB7_002433"
     CDS             complement(3135..3926)
                     /locus_tag="ACHBB7_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9210335.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3919..4692)
                     /locus_tag="ACHBB7_002434"
     CDS             complement(3919..4692)
                     /locus_tag="ACHBB7_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9210336.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4848..>7327)
                     /locus_tag="ACHBB7_002435"
     CDS             complement(4848..>7327)
                     /locus_tag="ACHBB7_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9210337.1"
                     /translation="YDFSAQDIAMKFCIKRNVASHYLNQLFSDGKLLKNDSVRPVMFK
                     YNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLPLIIKGNSGVGKSFLASLIY
                     QYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKKGAFTGAEKDTAGMLSSANG
                     GYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEYANVRLILATTEDKNTSLLA
                     TFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITIDSKIFCSIELFTDLLSKTFE
                     GNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWIDPEISIDLKKMFLKSLSEI
                     DLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESDEFIALRNYVVNKISPIIDS
                     TGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFRMKNDKQKLLANEIWSSLEV
                     EESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITASAIARETNKLLNTYVYEAFD
                     MPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVS
                     TALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKV
                     KDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTTEFYIQGIDCLNLEN
                     LINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRFLDSKKVFNEIEKVLYL
                     YEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFS
                     EIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         2366 a         1435 c          950 g         2576 t
ORIGIN      
        1 taggagtgag catttttcga aaaatactaa aaatttttca aaataaaaaa aggcatacag
       61 acaataaact gtctatacgc ctcacagact ctattctata catctaattt tggctgtcct
      121 gcaaaaaata tgtgttagcc tccatcgaaa gatcaaactt tggaaaacat cattataact
      181 ttgcttggta agagctttgc taaaacatga tatgacttgt atacaaaatg tggagtgtat
      241 tccaataaat aggaaggatt acaaaatttg aaaccaaata agtaaaaaat actgtcactt
      301 tgaattaatt tgaaatagta aattctcatt aaactattga aatgctgata ccaaaaaatg
      361 aagtactttt cgttcatacc aacttagagc acttcgttat ctatttattt cttcatttgg
      421 cttatctcta taatctcatg atttggtccg tagaaattaa agtaacggat tccattttcc
      481 cagaacggta attccataat atttttatct aataattcat atcctaaatc ttgaattgaa
      541 ttgaatacct catctatgtc atccgtatct aatgctagat gatcaatggc tcctctacac
      601 tcagtagttt tatcttccca tatttctatt atcgttccat tcaactcgaa aaagccgact
      661 ttacttccca cattcatttc ttcattaatc agactaaacc ctaaggattc ataaaagatt
      721 tttgaagctc caaaatcttt tgttggaatt ccaatatgtt gtaaaccatt taatttcact
      781 tacatcatcc cttcaattgt tttaaaatcc acattcataa tttattgaat agtctagttt
      841 atctaccttg attgctccct ccgtaacagt catacttaat gctgttccga ctttatcata
      901 cattctagta cttaaagcaa cattatcaat atataaaaca gctatcgtat catcaacaat
      961 taactttagt tcatattctt tgcctatttc caaaaatagt ggtcgttgca gccccttatc
     1021 aaacatttga ggccaaggat agttcggagt ttttccgaaa gtaagctgat tattaatcaa
     1081 tgaaatacga aattcgtatc cttcttgagt atcactatct acaaataatt ttaaagcaaa
     1141 ctcctttgtt aacgatgtaa tagtcaaagt tgtttcaaat agaaaataat cttcgatttt
     1201 aggaacaatt actctttctt tttttccaaa ttctttctct aacttaaaac ttgaaaatgg
     1261 agtgatttct ttaaaattct caattatttt ttcaggcagt ttaacaccaa gagttttgtc
     1321 tgtcttttga ataatttgat gtggaacaag tgcaccgccc caatcccagt tattttgatc
     1381 gttctcccct tcttttgttg ccacccagcc agttagaaaa cggccagttt cattagttcc
     1441 gactgttctt gctgcataat aggcttttcc gtcgaaagct tcgtctactg gtgatttcca
     1501 atctgaaaat aatcctgctc cgactcgata tttagtcttt ttatcttcac aatactctga
     1561 aaaaagtaaa taccacgttc catcaatttc aaatatatca ggcatttcaa tcatgttaaa
     1621 ttcattatta gcccagtagt ctcctttgaa attccaatct tttaaatctt cggattcaaa
     1681 atagaccaat ctccccgtcg gctttttctt acttgctggt tttctagctc ctagtatcaa
     1741 aacgtatttt ccatgatctt gatcaaaaat tgcaaaagga tcgcgccaat cattcttatc
     1801 atatccttct tgaggaacaa gttttgctgc ttcgccatcc tttttccaat gaattaaatc
     1861 tttagaactt gcaatcatta acacttctga agtttttcca tccaacttag ctttgcgatt
     1921 atgtccagta tacaaagcta aatactcatt gattccttgg acaatacttc cagcgtaaat
     1981 gtattgatca atgtcatcgt tgccaccacg atgaattact tccccgtatt cttcaaattt
     2041 cacaaaatca gtggttgtaa ctagtgacca cccaaaagga tcagttaaag gtttaggatt
     2101 tctcgtatct cgttgatgaa atagataaaa agttccatct ttataaaaag gcatgcaatc
     2161 cccaaaccaa aaatctttag gcttgtaaaa aattttttgc atttatcact catcctcttc
     2221 gttagtatgt ctttgcatat ctgcaagaca cgcaattgta ttcatatttc ttagattttc
     2281 gatgatatca tcgctttttt ttccttgtaa tctcaaattg acagcctcaa tcaaaattcc
     2341 taaattcatg ccaccaagaa gttgaaaccc attttgaaat acttcacttc ttttagcaaa
     2401 ttcgttgaaa ggtgttccac caatcaagtc agcaaatacc ataacttctt gattttttac
     2461 ttgagaaact attttttcaa tgttttcacg gaattcttca atgcctgcat ctccaagtgt
     2521 taccacatga caatttaaat tatcgccaaa aaacattttt aatgtatcta caaatccaat
     2581 ggcaatattt ccatgagatg taattataaa ctccatccac tcatcctctg ttcttaataa
     2641 ataaaatcct ttaaaaattc ttttgaatct ctaggagttt gtatcactgt cacttcaact
     2701 ccatcactaa ttagttcttt aaaagcctct ctctcttctt cagtaatatt tatcgtcgga
     2761 cggatgcgtt ctgttccatt ccttcttgat aaatttccta cgtttacact tgttattggt
     2821 aaaccgttat caatcaaaaa tttaacatcg attggagaaa ctgcaacaat aagaactttt
     2881 tgtccttcat atttaccaga aagaatattt ttaactgcta cttcccttgt aattattgaa
     2941 gttctaacat taggtggtgc agcgaggcga actacagctt tttttacgtc atcattggct
     3001 acttcattat taataaccat aattctgtta gcactcaatt gatttgtcca ttgtgttgct
     3061 acttgtccat gaatcattcg atcatcaata cgaacatgta taataccttt cattttttca
     3121 tccatattca acacctactt caaaatattt aacgctctta acgcaataga aactactaag
     3181 attaatagaa ttacataaac tggtttaacc tttcgtccta acaaataata cacaattgct
     3241 actacacatg caggtaataa accaggcata acttgatcaa agatttcatt tccggtcatt
     3301 gttaagtcgc cttgtttaaa ttcaaagcca acattcactt ttacaactgt ggcaatgagt
     3361 gcaccgacca ccattaaccc taaaattgat actgcatcag taatagactt taattttgtt
     3421 tcaatttgag ttagtaggct cgcacccgat tctttcccag caaggtataa aggataagca
     3481 gtaatcctta ataaaattga tgccagtatc cacaaaataa ttccaacaaa actaccttcc
     3541 aatgccatat tagctgcaat tgctccaaaa atagtccatg gaataactac aaatacacta
     3601 tctccaattc ccgctaaagg acccattaga cctgttttca gagcacttac tgcttccaaa
     3661 gacttatttt tttcttcttt ttcaattcca acattcatac caataatgta aggcgctgta
     3721 tacggcgtag tgttaaaaaa ttgtaagtga gctaaaacgg attgtttcat tccttcagga
     3781 tcatctttat atgtgtcctt gataaaagga agcatagaat acgcataacc caacccttcc
     3841 attttttcat aatttaatga agttgcacca ttgagaatcc accggaaaat tacttgtttg
     3901 tcgatttttg tcagtttctt attcatcttc gtcaaaacct cctgttgcag ctggtgctac
     3961 ttgagtatta tttgatctat ttttatatac gattagtgcg attgcagcgc caagtaacga
     4021 aatccctaaa attggtattt ggaaataaac tgccaaaacg aaaccaacta acaaataggc
     4081 aaagtaatct tttgtaggta aatatttaag tagcattcct atcccaacag caggtaatag
     4141 tccccctgct acctttaaac caccggtaac ccattcagga atataattga ttgcacttcc
     4201 cactaacgtt gggcctaata tcacagttaa caaaacagga attccagata caaagcttgt
     4261 attaattgat gccaaatatt gcatccactc taatttttga taatcttctt tttgtgcgaa
     4321 tttttctgcc ttttgttgaa aaaaaatgtt tgttgaccac tttagaacat ctagttgtac
     4381 aattaacatt gctaccggta ttgctaaagt tattcctata gaagcatcct ggttagttga
     4441 gattgctaaa taagtaccga tcaatgctgc tgtttgataa tccggtactg atgcaccacc
     4501 aaaactagta atacctagtg tcattaattg caaagtccca ccgatataaa gtccagtttg
     4561 aatatctccc ataataagtc cggtcaagaa acctgcaacg acaggttggg aggttccgat
     4621 tttgggccct tccttatccc agttaatgaa aaatccatac aacacaacta aaaatgtttg
     4681 ccaaaacgcc attttgttct ctcctttctt cttatacttt tatataagca attactgtgc
     4741 caactaacat tatccaataa agcgggaaaa tacagtaaat attaattaaa tgagcgtcta
     4801 aatatacttt ggacaccctt tcattttttt agacgctagt atgctattta gaaaatagtc
     4861 tgctcaaaaa ttgtatagat taactcatac tctcctttgg gtatttcaat acagtaaata
     4921 tctttgattt ctgaaaaaaa atcttttgat acatttaaaa aagtatcaaa tagttgcgct
     4981 ccctctttca aatgttcatt tgttattcca tcaccaatca taattcgttc aatcattcca
     5041 gaaagatgta gaaatagatt aattctcaga aaacttggga ttttgacatc gtaatatttt
     5101 tcatacaaat atagtacttt ttctatctca ttgaatactt ttttggaatc taaaaagcga
     5161 agcttagtac tagcgccttc aatagtaaag agtttaacta attcatttaa gcagaactca
     5221 cagctatccg agtccatata cttatttaat ttttctaagg tttgattccc attaattaga
     5281 ttttctaaat ttaaacaatc gattccttga atgtaaaatt cagtggttga aagaacacaa
     5341 attgtattct taaaaatcat agtatctttt tcctttgcta ttttctttaa tgcgttaaaa
     5401 tcaaaaatca aaatctccac ttgattatcg agataccgtt ttaaaatatc cttaactttt
     5461 tcagaaatcc cctcaccaga gagacaagat actattacat ttttcttttg tgataatcct
     5521 ttataatact gaactttaac ttgaaacgaa tcaaaatcca tctggtaaaa atgactaatt
     5581 ggcttttttt ggcaaacttg aatcccacat tcaatcgcta aagctgtaga aacattgttt
     5641 aaaattacca aatcacctat tacattggat tcaatttttt catacatttg ttccaatgat
     5701 cccatatcaa ctagcagaat gagtccattc gtcgtatcta tacttttaca aaaattgttg
     5761 attaggttaa ttaagtcact agtctcacct tcaattggca tatcgaaagc ctcataaaca
     5821 tatgtattca gcaacttatt tgtttccctt gcgatcgcag aagctgttat tttaccgtgc
     5881 ataactatca gacaatgggt ttctggtatt ttcacataga acttctttac aagaaaaagt
     5941 atccatttca acaaaagttc ttgtttactt tcttcgactt ctaaactact ccaaatctca
     6001 tttgccaata atttttgttt atcatttttc atacgaaatt ttctgaactt agacagcaaa
     6061 aaatttcttg atgtttcttc aattacatca ataaacatta taaatagact gatatccgcc
     6121 aagaattcat ctaataaaca tagacctgtc gaatcaataa ttggtgatat tttattaaca
     6181 acatagtttc ttaatgctat aaattcgtca ctttctaaat aattatactc agaagaacct
     6241 gcacactctt taagtattct atctcttaat tgactcatgc tttcttcaat tgaaatgtga
     6301 ttttgaaaaa gttcaatcaa atcaatttca gaaagtgatt taagaaacat tttttttaaa
     6361 tctatactaa tttctggatc tatccaaaaa ccgctgtcta gtttgtcccc taaataaatt
     6421 gaagactttc tcttatttcc taaataacct tctgcacaaa gaagcttgat ttcattcctc
     6481 aattctccaa catttccttc aaatgttttc gataaaagat cagtaaaaag ttcaatcgaa
     6541 caaaatattt ttgaatcaat cgtaattgct tcatctttaa gaaattcaaa taataattga
     6601 attttttcag atgtacttct attattgtaa tctggcagag tgatttcagc tgggatgcgt
     6661 cttctaaaag ttgctaacaa agaggtattt ttatcttctg ttgtggctaa gataaggcga
     6721 acatttgcat actcccattg cacactttca ccaagtttac gatacttttg agaatccata
     6781 agaagaaaca atttctcttg gttttctgca cttagattgt gaacctcatc taaaaaaagg
     6841 tatcctccat tagcagaaga tagcatccct gcagtatctt tttcagcccc agtaaaggct
     6901 ccttttttat atccaaataa aactgctgac aatagctctg gattatttgc ataatctgca
     6961 caattcacaa caacaaattt tgcgtcatta tgaattactt ttctatcaag agcatactga
     7021 tagatcaaac ttgctaaaaa actttttcca acgccactgt ttccttttat tattaatggg
     7081 aggccattag ggggatacat tacagtagct ttgcatttat ctatagtgga tttcaaagat
     7141 atgtctgctc caataaactt agaaaagcag tcttttggct tttgttgatt atacttgaac
     7201 attactggtc taacagaatc attctttagt aattttccat ctgaaaataa ctgattcaaa
     7261 tagtgggaag cgacatttct tttaatacaa aatttcattg caatatcttg agcagaaaaa
     7321 tcgtaat
//
LOCUS       DAYYKN010000135         7225 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000135 DAYYKN010000000
VERSION     DAYYKN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.137"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..1373)
                     /gene="celB"
                     /locus_tag="ACHBB7_002436"
     CDS             complement(21..1373)
                     /gene="celB"
                     /locus_tag="ACHBB7_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM9210338.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1373..1891)
                     /locus_tag="ACHBB7_002437"
     CDS             complement(1373..1891)
                     /locus_tag="ACHBB7_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210339.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1907..2233)
                     /locus_tag="ACHBB7_002438"
     CDS             complement(1907..2233)
                     /locus_tag="ACHBB7_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM9210340.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2246..4225)
                     /locus_tag="ACHBB7_002439"
     CDS             complement(2246..4225)
                     /locus_tag="ACHBB7_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9210341.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4246..4560)
                     /locus_tag="ACHBB7_002440"
     CDS             complement(4246..4560)
                     /locus_tag="ACHBB7_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM9210342.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4728..5021
                     /locus_tag="ACHBB7_002441"
     CDS             4728..5021
                     /locus_tag="ACHBB7_002441"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM9210343.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5099..6211)
                     /locus_tag="ACHBB7_002442"
     CDS             complement(5099..6211)
                     /locus_tag="ACHBB7_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9210344.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6364..7212
                     /locus_tag="ACHBB7_002443"
                     /pseudo
     CDS             6364..7212
                     /locus_tag="ACHBB7_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2326 a         1452 c         1180 g         2267 t
ORIGIN      
        1 agttcctttt ttcttagaaa tcattttaac ttttcactag ctaattctgc tgccttttct
       61 ctttcttcat ttaataattt attatcatag aatttgataa atgggaacca gataatgaat
      121 gcgatgattg cacaaatgac tgctaacaca gcagctttcc aatcaccacc actaccaaca
      181 aatgctccga tcccgactgg tgaaggccac ggcatctgtg caagcatcgg tttgacgatt
      241 tccagccgaa tggcaaaata agcgagtgta gcagaagcca tcggagctaa gaaaaacgga
      301 accgctaaat agggattgta gacgattggc atcccgaaaa tgattggttc attgatattg
      361 aataatcctg gtacaagtga ggcttttcct aacactttca gctgatcaga tttcgcgagg
      421 aatgcaataa agatgatcaa tcctaaagtc gcacctgatc caccaacggt tacataagaa
      481 ttgttgaatt ctcctgccaa cggataattt gcgccttgtg cgttagctgc catattagct
      541 aatacgatcg gggtcaagaa agaagtgatg atattcgctc catggatccc tacgatccac
      601 aatgcatgga tcaagaaata aatcaccatg acaccaagcc atgtattcgt caactgcgta
      661 acaaacccaa atgggatagc aatcattttg aagatatccg ttcctagtgc aactaataat
      721 ccattgatga tcatcaccac aaacgcaatg acaaatgttg ggatcagagc agtgaaagaa
      781 cgagaaactc ctggcggaac aacatctggc atttttatga cccaatttct cttgacacac
      841 atacaataca attcgaccgc aataatcgac ataataatag ctgtaaagat tcctgaagtt
      901 cctaaacgtt cgacaaaaga gcccatccta ataccattta ctacagtttc tgtctcattg
      961 atagaagcaa tcaagctgat tttaccatca gtcatgatca attcagggat acacataaag
     1021 aatgcaaaaa cagatagtaa tgcccccgtg agcggattaa catttaattt ttgttcatct
     1081 cgttcaattg acgttaattc ataagcaaac acaatggcaa agtaaataga taaaatcccc
     1141 atcgtcattg tattgacgat catgtacaag tcactaactt tgaaaaaagt tgcattaaaa
     1201 aatccttcta aaaatgggaa cggtaatggc aaaatattta aaaccaaaaa catcgagcct
     1261 acaatcgtga aaggaataga agccatcccc gctgccatga cagcccgaac gattttaagt
     1321 tgcgcaattt tccccatagg tcccatcagg tatttctgca aaaaatcaaa cattagttat
     1381 tctcctttcc taaatataac gctttttcat attgtttaat acggatatac tgttggtctt
     1441 ggttgagtga aattttcttt aaacgatact gtataaatgc actggcaaaa ataccgctta
     1501 caacaagaat gaaaacaaat aactgtcctc cattgttatt tgccataaaa ggatacaaga
     1561 atgtaaatcc tgaggtaaac aacactggaa tcactaataa attaaagaca ctctggaaca
     1621 aaaaatacca ctttgtccat ggaacgttcg tttggtgttt tcgataaagc tttacttgtt
     1681 ctcctactgc aggcaaaata gctaaaatca gaatcatcgg taaactactt cctaaacttt
     1741 tagctgatag aagcagaatc atccattgca tgttcatgaa aaagaaaaag gcagtcaggt
     1801 aacgtacgat tagataacga ttgaaataca tgtttttcaa actaagttgc tttttttcta
     1861 attcgatttt cctttttact gtatcctgca caatgatcct tcctttctat ttattttttt
     1921 gatacaaata attcatttcc tgtgcaactt ccaataatgt catcgtcgtc atcagatgat
     1981 cttgtgcatg gaccatgata atttccattt ctatttttgt cccgctggca tattctttca
     2041 gaagttgagt ttgtgactga tgcgcatgaa gaatctcttc atttgcttgt cgcaacttct
     2101 cttctacttc ttcaaacatc tctttacgca tcgagcgaaa tgcttcatgt atcagtgtcc
     2161 gtgcatttcc gctatggaaa atgatatcaa aagaagtcac ttgtagtgtt tcactgttta
     2221 ttttttcagc catctgtttt cctccttatt cgatcagttt gataaattta ctgctgaaat
     2281 tctcaaaaat aggttcagct aagatttcct gctgtacttt tagctggtca actaacctaa
     2341 cgattgcttt agtcacgacc gtaattcctt cattttcgac ataagaaggc gaaactaaaa
     2401 aaacaaaatg aatttcttga tattgatccc actgcatccc agaaggaatc aatgcaacgg
     2461 ctacctttgt ttcgattcca actggaacag cgggatgtgg gactgccact gtatcactga
     2521 atatgatttg tcccatattt tcacgttgct ggatctgctt tcgcatttct cttatatatc
     2581 cttcttcttc atttatgctc agcttttgca gcaaatcatg aagaatttct tcctttttcg
     2641 gttcgtacgt ataagtcttg aaaaattttt cagacaactg ttcggtgtaa atctgctttt
     2701 tttgctgtag agaaaatgct ggtatcggtc tatctgagga atgatagggt atagattctc
     2761 caactacctt tcgtatttta cgaacatcat cttcattcaa aaaaatactg acatgtaaaa
     2821 ctggaatttt gaaaaacatt gtcgaaagat caatagaaga aataatcagg tcgattcctt
     2881 ttaaagttgt ttcattgatc tcataatagc cttttacact taccactgta atatttttac
     2941 cgaattcact gactacgcga ttctttaata gttgggcact gccgtaaccc gttgcacaga
     3001 taatcaatgc atgtagttta tgcgcagctc tttctttttc caatgccgcc atgagatgca
     3061 aagccaaata ggcccattca tcttcattga tggtatagtt tttcaaagcc ggcatgttgc
     3121 tgaaatgcct ttttactgcg tcaaagactt tgggattttc tttctttatc tcctcagtca
     3181 atgggttctc aatgcgaata cctcggctca atcgaatcat catcggtgac aaatgatcga
     3241 gtagtccatt gaaaagctgg agatcctcta ctaatgaaaa atgaaattcc tgagacaatc
     3301 ttttcagaac tttcgctaat tcctttgata gttcctgatc attttgattt tctttgtgat
     3361 tcgacttagc catgagatgt aatgtcaaat aagcaatttc ttcttcggga aacgatacac
     3421 ctgcgattac ctcaatacgt tgaacgatcc gctgcgccac ttgatactct actcgcttag
     3481 aaacttcttg ttcgatgcct aattccttga tttcaaaccc ttctcggagc cgtttgatcc
     3541 ccaaagccaa gtgtaaagtc agattctgta tgataaaatc cgaaactttt aacttagctt
     3601 ctcttgtttc atcgagaatg ataatgacta gctcttcaaa actgatgtct gggaaaaaac
     3661 ggctttgccc taaatattct ttcaatgaat ttccataatg gtttccaaaa aaagtatcca
     3721 tgataaaatg ccgtcgattt ctttcttccc cttcgatcca tgtcccttga ccatgcttag
     3781 aaacgatttt tagtgaatat ggaatcaatc gctccttgat ttcttgcatg tcctttgtca
     3841 aactggaacg actgatataa agctcttctg ccaaatcatc cataaaaaga atggcatctt
     3901 ccaaaagcaa ttggttcaat atatactttt gtcgatcttc ggactcataa aatccttgcg
     3961 gttctctagc atatccaggt actatttctt gccgactcaa aaacaagtca aatgtcagtc
     4021 tttgtaaaat atgcagttgg tagccataac ctggtttagc aatgatctta cctccgtttt
     4081 tttctactaa ctctttgatc cgctgcaagt aattacggat tgtccgatca gataataaca
     4141 attcagaagc taaacgttga ctggtgacat actccttctt gtgatcaatc aaatatctca
     4201 acagcaattt ttccttctgt ttcattatca tccactccta ttccatcata tgttctcttc
     4261 gatcacctct gctaacttct ggatgcctgt agggattggg acatacgctt ggaaagggat
     4321 actaacaaca ggctttccaa cagattctcc taattttttg aatttatcca aaaacatcgt
     4381 cgtttgcggg ctgatcagaa ataaatcaaa atcactattc ttaatcatat tatcgccttc
     4441 tgtagctgaa actgcatcta cgagtacctc ttctcttttt tgcttaaaga aatccgttgt
     4501 ctttgaggcc atcatcgagg atgacattcc tgccgcacaa ataatcaatg cttttttcat
     4561 aaatcaatca ctccttttat ttttataaac caagcatatc ttgtaagcgt tttctattca
     4621 aaatatatat ttccgtagca ttgcggaaaa aatgtgtatc atatcttgaa taataatttc
     4681 tggcaccgtg atatactagt gatagaattt tttcaggagg ggatttagtg agtatcacag
     4741 tgaatatttt gtatacaagt acaaatggac aagccagaaa atttgcagaa gaaatggtcg
     4801 aaagaaacat tgtcaaagcg attcgtgcag aagaaggaaa cgagaaatat gactattact
     4861 ttccactaga agatccagaa tctcttttac tgatcgaccg ttggaaagat gaagaagcaa
     4921 tcgaaaagca tcacaaatcc gaaatgatgg ctcaaataaa cgaactccgt aaaaagtata
     4981 aattaaaaat gaaagtcgaa cgtttcactg atttaccgta aaaagataca catggaatga
     5041 tagcaattta tcatccatgt gtatcttcac tttttcctta ttttttattc gatctcatct
     5101 aatgattcaa accgtgtttt tctcttattt ttcggataag tatttcagtc accccaatat
     5161 acatgattcc taaacatacc ccaaggatat tctcaaaaat gcggatcgtt actgctcctg
     5221 gaaccccgaa taaactggca gtgattgcca aagcaccaaa agaattaaaa actgttttat
     5281 atctataggt agaacaaaca cctaaagcta gaccacctaa tataccgacc cagttaaaag
     5341 gaataaattg gctgatacca ataaataaaa cacatccaat caatgagcca atgattcggt
     5401 ctactgctcg ttctttgaaa ccaatcgaca tcaatccata gctagataca atagaagaaa
     5461 atgcaaaccc tgcccacatc aatcgctgaa aagggacata ctctcctgca acgatcaaaa
     5521 gactaatgcc aaatgcatag atgagcatcc aaattttttc ctgcgaatac atgcctttca
     5581 aaaaaatctc tttaaacaag cttttgtctt tgttcttttc tcgatgtttt ctgtaaagga
     5641 gaactgaaaa ccaacaaaaa aacaaaaata ataagctact tgtttgtgta aagaaattct
     5701 tgttcaacga atctttaggt aacgaataaa caataaagag atatgaaaag ccgtacaaag
     5761 aagcattccc cattttggga tcactagcgg ttgccattaa tatgctgctt agtaaaataa
     5821 aatgaatcaa catgcccata actgaccatt tgatcagctg gacatacggt gctactaata
     5881 gtgagaacat gacgattgct aaacctatga tcgaatggga aatcttataa ccaaagtcaa
     5941 caaaacgaat actcatcaac atacagaata aaaccacagc gtatggttta tgcttcgatt
     6001 caaataatgt tgtaattata atgataaaac aaatggcaaa caatacgatg aataatgatc
     6061 gtaaaaacaa tgaacaaata tacatgcact tttctttttt agaagctgct tctcgtattt
     6121 tttgttttaa aataaacgga tctagttgta gagactgata aaaatccacc tgttgtcgtc
     6181 ctttcttttt acttttattt tttacagtca tctatgacag gttgtaaata ctatggaaca
     6241 tcatatggcg ttgtcatttt cttgtcaact atttttccta ttcatgtttt tataaaaaaa
     6301 catatcgttt tcttgtatga ttaaatcacc acaaaataaa caactgagag aacgatatgc
     6361 tttatgaatg gttctatcaa aaaaattctg agatcaatag acaaaaattt gatgattaca
     6421 gaggtctctt acgaaacctt acagaagaaa aaaacattga tcgttgctgc agttctcttc
     6481 cctgctccat gtgtctgtag aaattgtggt tctgctgtgg tagatggaaa taggaaatta
     6541 atggtggtga aaaacaagaa gaaggaaacc attgtccact ttgaataata taatcatatg
     6601 ccaatggtta tacgcctaaa aaagcagcgc tatacctgta aaaactgtcg aagccattgg
     6661 aacgctcaaa gttattttat ccgtccaagg cactcgattt caaatcatgt cagacataaa
     6721 atcacttctt tactgactga aaaagtgtcg ttatttttta tttcgaaaag ttgttaggta
     6781 tctttgacaa ccgttattcg cacattgaaa gagttgagaa gctatttacc aaagtcttcc
     6841 aaaagaattc tgtcttgagt gttgagggtt gtgaatttcg ttctcacgtt tccgttgagg
     6901 ataaactgag ttttatttcc gcggatggcg agacagaaaa attaatcgag aaaatttacc
     6961 tacacaaaaa attcctcgat taacaaacta tttccttagc tgcactaatc ctaaagaaat
     7021 agaattctta gttacagaca tgaacgccac ctactttcag ctcaccaaac atgttctacc
     7081 aaatgcgaaa ttggtgattg atcgattact tggtttttcc acgcctttca aggaggcgta
     7141 acctttattc acgagttggt tgaagctttc cgagacaaaa gacccgactt gtttttctcc
     7201 ttattgacaa agagccatta aaact
//
LOCUS       DAYYKN010000136         7153 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000136 DAYYKN010000000
VERSION     DAYYKN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.138"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1368)
                     /locus_tag="ACHBB7_002444"
                     /pseudo
     CDS             complement(1..1368)
                     /locus_tag="ACHBB7_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1611..2066
                     /gene="tnpA"
                     /locus_tag="ACHBB7_002445"
     CDS             1611..2066
                     /gene="tnpA"
                     /locus_tag="ACHBB7_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM9210345.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2331..2429
                     /locus_tag="ACHBB7_002446"
                     /pseudo
     CDS             2331..2429
                     /locus_tag="ACHBB7_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2652..2882)
                     /locus_tag="ACHBB7_002447"
     CDS             complement(2652..2882)
                     /locus_tag="ACHBB7_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM9210346.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(3112..3930)
                     /locus_tag="ACHBB7_002448"
     CDS             complement(3112..3930)
                     /locus_tag="ACHBB7_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9210347.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4091..4780
                     /locus_tag="ACHBB7_002449"
     CDS             4091..4780
                     /locus_tag="ACHBB7_002449"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9210348.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4794..6296
                     /locus_tag="ACHBB7_002450"
     CDS             4794..6296
                     /locus_tag="ACHBB7_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9210349.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6309..6785
                     /locus_tag="ACHBB7_002451"
     CDS             6309..6785
                     /locus_tag="ACHBB7_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9210350.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6882..6992)
                     /locus_tag="ACHBB7_002452"
                     /pseudo
     CDS             complement(6882..6992)
                     /locus_tag="ACHBB7_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2318 a         1131 c         1294 g         2410 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggatccg atttttggct actgctccga cttgtcctcg ataagaattg tagcgccgtg
      541 atttacgtgt gtatgtcgtt acttttattc ctagcttctt gatcaggcgc tgaactttct
      601 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
      661 ccttgtgctt ctcccgaact ttaagcattt ccgcttcaat ctcttgatct ggatttttac
      721 ggtccaatcg tttttgccag tacataaaga ttgccttagg gaactttaaa gtttctagaa
      781 gctcaactaa tttaaacggt cctcggaggc ttgcgatgat tcgtgcgatt gcttcattcg
      841 tcgttgttgg gcttcctgtt ttcgcagctt cctcaattct tttaaaaagg cattttcaat
      901 ttgaagggaa cggactcgtt tctccaactc ctcgatgcgt tcacgttctt tagacgttgc
      961 tttagttgta gattttttct tatttttggg ttcatttttc ttagacaaga taggtggaca
     1021 tcctttcgtt tttgagagtc catcgatccc accctcacgg aattgacgca tccaactagc
     1081 gatcaaatta gggttgttca tgttaagtgt gttggcaact tcacgatagg acagctcact
     1141 tgttagatat aactctatcg catccagctt aaattgaaca gaatattttt tcttttgtcg
     1201 ctttcttact agaccctctt caccaaattc ttgataggca ttgatccagc gcttcacttg
     1261 tgatggagcc ttaatggaat atttctttgc cagataatca cttccgcctt taccatccaa
     1321 gtaatcatga acgatcttta atttaagttc aaaagtatac ttagccataa aaaataccga
     1381 cctccaaaag ttagattttt tggtctaact tttgggggtc ggtacaaaac ttaaaagttt
     1441 tagacccttt tattttctta atgacttcag tcagttttgt gcgtaaagcg tgcgaaacaa
     1501 gaaaaccgcg cgtttctacg cgtggaagaa gtatctttag cgataaaaac tcctttcgat
     1561 atactagtga gtggctacca accgctcatc agatcgaaag gagttttttc atgtcaaatg
     1621 acgataaaag tttagcatat actcgttgga attgtaagta tcatattatt tttacaccaa
     1681 aatatcaacg aaaagtaatc tatgggcaat tacgaaaaga tcttggaaaa atacttagga
     1741 agttgtgcga aatgaaagat gtggaaatca tagaagcaca tgcaatgcta gatcatattc
     1801 acatgcttgt tcgagtacca ccaaaaatat gtatatccag ttttatgggg tatctgaaag
     1861 gtcgtagtgc ggtactaatc cacgagcagc acgcaaatct caaatataaa tacggaaaca
     1921 aatccttttg gtcgaagaga tactatgtga gtacagtcgg attgaatcaa aagacaaatg
     1981 aaaaatacat ccgtgaacaa gaagctgatg atcgcattcg atatattata agtaaaagag
     2041 agtacgtaga accatttaaa aaataaagag aagtaagtga gtgaagatga gcagttggcg
     2101 gtcttttaaa aatccccttt taaggggcag cgagtgtcga ccccttctag ggggattaaa
     2161 aatacacccg tcctcacggg tgtatgggtt actgatagac ttgattttaa ctggtgtcag
     2221 aaacttatgt ccagaaaggg tgatgaaatt ttggcaattt acggctattc aagaattagc
     2281 acaagcaacc aagattacaa aacacaaatt caaacattag aaaatgctgg cgcagaaata
     2341 atcttcctag aaaatcactg gattatcgaa cacctttgga agtatttttg agttacgtaa
     2401 gtattgatga tctgtctaac ttaatttgac aattaagagt atatttactt cagatggtaa
     2461 ggcaacaaaa taaaatacaa taggcagatg aactaataaa taaaatctgt attaaataca
     2521 gataaattaa tataaaaatc aaaaggatca taaactttta acaagtgatg aaatgggtgg
     2581 aaattgattc ttatagacag tttgtctcat aacattgaag tattttgcaa ttttagcatt
     2641 gaaacacttt tttaatcttc aacgttgttc ttataaacaa gatgattggg aatcttttga
     2701 attaacactg tatagcttag aacacccttt atagaaaaca ttcgcttagc aatcttaata
     2761 attccttgtg cccgatgtcg tttagattca gtaggttcct gctcatcgat catggaaaaa
     2821 atttgaagga tcagatcctt aataaattta tttaagagag gatgatccat tgcttcatcc
     2881 atactcgata aactagtgat aatgagctac acgtttttct ttcaagttga tcaatagctt
     2941 ccacgataaa aatatcttta gataaaatca agaaccgtag gaactacagg gatatttggt
     3001 caataagagc caccgctatt agtaaaaaat aagctatcaa gtatgctctc atcccaagaa
     3061 ttgcctactt caatcgttaa aaaccgttag gtttagctaa gtgggggtag ttcactcaat
     3121 acttttatca ataatagagc gcaaaacttt ttctcttaat tcaactgaat caatattatt
     3181 gttttgttca taaccatgaa ctaatagatc acaaatataa agttgtgata tttttccagc
     3241 taaagatcca ccattaagaa attcttcaat ggctgtttgt aaaactaagt ctgcagactt
     3301 tcctataggt gaatgaatat agttagttat agcaataatt tttgcgccat tttttttagc
     3361 tattttcaat gaatcataag tatctttcgt tttcccagat aaagaaaaaa tgattactaa
     3421 atcattaact gttaataaag aagcgacttg ggcttgaaaa tgagggtcta gtacagcttt
     3481 tgcttgaact cctacgcgaa gaaacatatt ttctaaatca agactagtat ttccactaga
     3541 tcccactcca aatatatata tgttttttga ttgagtgatc attttaatgg cttcattcaa
     3601 tttttcttcg tctaaaagcc taatagtgga atagagagct tctgttaaac tattagcaat
     3661 ttcatcatag tactttacac tagtcggatg ttcttttttt tgactaaagt cttcctttgc
     3721 aatttcaatt tttaaatcag aaaaaccaga aaaaccaatt ttttgacaaa agcgaataat
     3781 agtagcatcg ccaacttttg tgtttttctt aatatcactc atcgtactat aaataatagt
     3841 tttcccagaa tttaaaataa aatcagcgac ttttttttca gacttcgtta aaaatggata
     3901 attttcttta attaaataga tataactcat acaaactcct ctacaatttc aacttttctc
     3961 cattatactc caaaataata aaaaaaactc ctaaatactc catttttatc tttactttta
     4021 tgaaagcgga tgctatattg tttttggagt tattatccaa aaataataaa aaattggagc
     4081 gtttaggata atggataaaa agaggttttt agatcttatt tcaaaaggtt tgattgtttc
     4141 atgtcaagca ttacctggtg aaccatttta caaagaggaa gaaagtttaa tgccttattt
     4201 tgctttggct gctcaaaggg ctggagcagt tggtatacgt gcaaattctg ttcgagatat
     4261 tattgaaatt agaaagtgtg tatcattacc aatgattgga attattaaaa gagattatcc
     4321 accagaatct ccattcatta ctgccactat gagagaagta gatgaattag tagaaaccgg
     4381 tgttgaggta atcgctcttg attgcactct tagaaatcga catgatggac tatctataaa
     4441 ttcttttatt actaaaataa aagaaaaata tcctcatcag ttatttatgg cagatatttc
     4501 tacatttgaa gaaggcaaaa atgcatataa agcaggaata gattttgtag gaactacttt
     4561 aagtggatat actgaggaaa gtaaaaaaca agatgggcca gatatagatt tgattaaaaa
     4621 attggtagaa gaagggattt gtgtaattgc tgagggaagg attcatactc cagaacaagc
     4681 aaaagagata aaaaaaatag gagttgcagg tattgttgtg ggtggtgcta ttactcgacc
     4741 acaggaaatt gcatcaagat ttgttaaagc attaagttag ggggagaaat aaaatgttta
     4801 aaaaattttc acaattagga cgcgctttca tgttaccaat tgctatatta ccagtagccg
     4861 gattactatt gggactggga ggagcgttaa caaatgagag tgctataaat gcttatccat
     4921 ttttagatca accgtggttg catacaattt taagtattat gagttatgct ggaaatgcag
     4981 tttttgcaaa cctagcacta atttttgcaa ttggtattgc tgtaggactt gcaaatggag
     5041 ataaaggaac agctggttta gctggtggag tagcttatct agtatatact gcaactatta
     5101 gtgggtttct tgaacttttt tcagcaaaag atgcaacact agatacaggt gtagtgggat
     5161 caattgttgt tggtggtact gttgcatttt tacacaatcg ttatagaaaa attgaattac
     5221 cacaattctt aggttttttt ggaggatcaa gatttattcc gattgtttct agtttagctg
     5281 caatcattat tggttctttc ttttatttaa tttggccacc aattcaaaat ggtttagtag
     5341 ttgctggtga acaaattgcc caaatgggta gtttaggaac ttttttatat ggttttttat
     5401 tgcgtttaac aggtgctgtt ggactacacc acaccattta ccctttgttc tggtatacat
     5461 ctttaggtgg taccgaaact gtagctggat caacaattgc tggagcccaa aatattttct
     5521 ttgcccaact agctgatcca aaccacactg gtctgtttac ttatggtact agattttttg
     5581 caggaagatt tgcaacaatg atgtttggat taccagcagc atgttatgca atgtatcgtg
     5641 caattcctaa gaaaaatcgt aaaaagaatg gtgggcttta ttttagcggg gcccttacat
     5701 catttttaac tggaattact gaaccagttg aatatatgtt tttatttgtt gctccttggc
     5761 tttatgtgat tcatgcattc ttagatggat tatcttttta ttttgctgat atcttaaata
     5821 ttcgtgtagg gaattccttt tcaggtggtt tgatcgactt tttattattc ggtgtacttc
     5881 aagggaatga caaaactaat tggttaaaat taattccttt tggtattgca tgggcattta
     5941 tatatttctt tgtttttaca ttctttatca aaaaatttaa ggtagctatt cctggaatgg
     6001 aagaagaatc agaagttcat tctgaaaaag taataaacaa aaatacatca ttacatgaag
     6061 agtctcttat cattattgaa gctcttggtg gaaaagaaaa tattgagtct gttgaagctt
     6121 gtgcaactcg tttgcgtgtt gctgttacga atggggagat tattcaaaaa caaacaataa
     6181 aagacactgg tgcaactgct gtcttcgaag tgaagggtgg catccaagct gtttttggag
     6241 ggaaagctga cttattaagt caagaaatca atcaaatatt aggaaaagat aattaggagg
     6301 tttttaatat gtttgaaatt ttcaaaaaga aaaaacaaga aactttttat gcaccttgtt
     6361 ccggaaaaat catttccatc acagaagtaa atgatcctgt cttttcacaa aaattactag
     6421 gtgatggttt tgccgtcata cctgatggtt caaaaattta tgctccattg gctggtcgag
     6481 taacaaatat atttcctact aaacatgcta ttaatataaa aagcaactca ggcattgatt
     6541 atttgattca tattggaatt gatacagttg aattacaagg agagcctttt tcgatttgtg
     6601 ttcaagaaaa tgatgaggtt actcctgaaa cctcgttggt tgatgtggat tttgacaaaa
     6661 taataaaatc tggtaaagat acttctgtta tagttgtttt tccggagaaa aatcagttca
     6721 atgatttaat aatagataat ggtcgaattg ctcatgggaa ttcatgtgga aaaatcactt
     6781 tttaaaaaag tgtatataaa aagctataaa ataagctaaa taggatattt gtcaaatgaa
     6841 gttggtgaac agaaagaact gttttctttc tgttcatttt tttattttat tagtaatgat
     6901 tccccatgcc aaatcatgat ccgtaattta aagttataaa agcttcgaaa tccattggca
     6961 tttcgttgga ataccttgat atgattattc aaatataaaa tatagagaga agagagagag
     7021 ggaagatatt tataaagggc aaagttatgt ttactcaact taggaaatag aaatagatca
     7081 atttatattc tcttgtcaat cgaatatgaa ccttagcact atataccaaa tatagtaaaa
     7141 tgctgaaaga tgt
//
LOCUS       DAYYKN010000137         7107 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000137 DAYYKN010000000
VERSION     DAYYKN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.139"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..1889)
                     /locus_tag="ACHBB7_002453"
     CDS             complement(114..1889)
                     /locus_tag="ACHBB7_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM9210351.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(2102..3004)
                     /locus_tag="ACHBB7_002454"
     CDS             complement(2102..3004)
                     /locus_tag="ACHBB7_002454"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM9210352.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            3283..5628
                     /locus_tag="ACHBB7_002455"
     CDS             3283..5628
                     /locus_tag="ACHBB7_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM9210353.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            complement(5964..6965)
                     /gene="ccpA"
                     /locus_tag="ACHBB7_002456"
     CDS             complement(5964..6965)
                     /gene="ccpA"
                     /locus_tag="ACHBB7_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM9210354.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT         2199 a         1413 c         1317 g         2178 t
ORIGIN      
        1 tagtcctcaa ggctttgttc gaactttctt tcactcttca ctataatcta aaaatagaaa
       61 atagaggaga acatcattcg tcaatgatgt tctcctctaa tcttagtatg tttttatttg
      121 gaagataaag cttttttcag attcaattta ctgaaaacta gcatgcttgt tactacgatg
      181 aattgcgtta atggcacgcc tgcgccagtg tagacactta aaaaataatc ctttttttct
      241 ggcgcataag aacaaaacca cgagacgccg aaaagaaaaa ccaattgtgt cagtattaat
      301 ccggtgacta caatcaaaga ggaactataa taggcacgat cagcttttgc aaatgcggtt
      361 cctagtatca taagtaatat ccctgctatt gaagcaatca gcacgggaat ggccagaact
      421 tggtaaattt ttatcatgac attcgcgact tttgctgttt ttgtcaactg gaaatcaggt
      481 gttctcggat cactgctatc gatccaatta tccattagtg gaagattcaa taattcttgg
      541 gcctgttcga cttgttcttg cgtaccgaaa ctataattcc ccccttgatg gaaatctttg
      601 tatcgccatg cataggcata tcctgatttc agaaaagcaa atacgattgg cagatcattc
      661 atcgtctttc catcccctga tcccatcaga tagaattctt ttttcttttt gatagttccc
      721 ttttgatagg cttcttctaa ttcttgattg acttttttcc aaaaatcatt ggtttctttt
      781 gcattgtttt ggtaataacc agattcgctt accacatctc gcaatgccca aaaaatgata
      841 tctcctggta attcttttac ttttccacct tgggtccacg catgagtcgt atacaattcg
      901 tcaatacgag tagaaacagc atgaaatgta ggagatgcgt ctaatgcttt atttaatgca
      961 gcatgcgaga cccatatctc actgttttct ttatttaagt tcgtttcgtc atctattcgg
     1021 atcagctgtt tcatcaaacg tccaaattcg ccactcgaac ggtcattgat ggcggcaata
     1081 ctatatgttg tctcgttttt atattcgatc cactgataga cgatggagaa accgactaga
     1141 gggagtaaaa acaatgaaag tttcagccat tcttgtttcg aaaggagaaa tggtcgttct
     1201 tttaacttct ttgcttcttt gaaagaaaag tcttgtccta gcaagatctg gacgaaactg
     1261 atgcctaacg cgactacagt aaaaggaaga agccagatgg aatcttcgcg aatgaaccaa
     1321 aagaaaggaa aagaaacagc tagccctaaa aaccacggca gcattttttt gctggaacta
     1381 tatttacggt aataaatagc tagtaaacaa gcgataatga tctcaacagc cggaatcacg
     1441 agggcattac gatacgttcg agtggaatat tcatgtgtaa aactaatcgg agaaaacaaa
     1501 aaataaaaat agaaacttaa aagaagatac ttgttcttca cgatcggttt aaacgcgagt
     1561 gcaaccaata tactggcaaa aatactgaac atgccgaaca aaactgcata agaaagatgc
     1621 agtttatgtg caagtactaa aaataagctg taggagattc ctttggaaag tgttgtagtc
     1681 gtatactccc ctagccattt accgtccaaa atattttgag catagcgaat cagcagctgg
     1741 tcatcatagg ttgccgacat gtctgttcca tagattgctt tactatataa atacaaacga
     1801 ataattgtaa ataatagggc tatgccaaga ataaagtatg tatgtttctt ttttagttgg
     1861 ttgatcaagg gttttaaagt gttcagcatc ttttcgtcct tccaatcacg ttttgccact
     1921 tgcttgtgaa acttttcgtt tttttacttt tctaccagta taccctgttt gtctaaattt
     1981 aaaaagaggg gggagaagat gcatgatgaa aaagactgca aaaagcactc ctttgtttct
     2041 taaacaaaaa aggacaaggc aatgtgcctg gtcctttttt gctgctgtca gatagaaaat
     2101 cttagagtat cacttcttct gctaataact caggaaacac ttctggatcg aatatttgtg
     2161 ctttgaacat ggcgatttct tttccatatg gtgcgtaagc tttcttttta tctttgccga
     2221 catagggagt ttccaagatt ttcgaaatat tgtcaaacat tggatcatgg acgatcttat
     2281 tcagcgtgtc gaatccgatt gttccaaaac caatattggc gtgccggtct ttatgcgaac
     2341 cttgcggatt tttagaatca ttgatatgga gtactttaag ccgatctaac ccgatgatct
     2401 tatcaaactc ttccattaca cctgaaaagt cttctttaat attataaccg gcatcattcg
     2461 tatgacaggt gtcaaaagta acggataatt tttcattcaa ttttactcca tcgatgattt
     2521 gcgctaattc ctcaaatgtt cgaccgattt ccgtaccttt acctgccatt gtctccaaag
     2581 cgatttgtgc cgtctgttct tttgtcaaaa cttcattcaa gccttcaata attctagcaa
     2641 ttccggcttc tgttcctgcc cctacatgag cacctgggtg aagtgtaatt tgagtagcgc
     2701 ctaaagcttc cgcacgaaca atctcttctc tcagaaattg gacggcaaaa ggaaacattt
     2761 ctggtttgat cgtattcccc aaattaatga tataaggggc atgaacgacg atatttgtta
     2821 gaccgttttc tgccatgtac gcttttccgg catcaatatt catctcttcg atggcttttc
     2881 ttctagtgtt ttgaggggcg cctgtataga tcataaaagt cgaagatcca taactggctg
     2941 cttcttctgc tgctcctaac agcattttct ttccgctcat gctgacgtgt gagcctaata
     3001 acatggatgg attcctcctt gaaaggcaat tgttactaac aattcctata tattggtaag
     3061 atgatataca aagaaaatga ttgatttttc tttgccctgt tctccatagt aactgaaatt
     3121 ttctctaaag aaaagcttaa gaactctttt ataaaaaaga tcattttctc acgttttaac
     3181 tcttttaaaa aagaaattcc tgccaaaaac agccttgtct gtttttttgt tctattattt
     3241 gctataatta gcgaaataaa accattagtt gaggtgaccc ttttgccacg caaaaatact
     3301 cgaaaaaaac agaatagaag aaaaaaagaa aaatggtttg ttcctaagat tatttttaga
     3361 gtttttcaat cattgactgt ttttattact gttcttcttt tgatgttcgc agctctaggt
     3421 attggaatag gcgctggcta ttttgcctat ctcgtcgaag acacacaatt gccgactaaa
     3481 aaagcactgc agacagaact aggaaacatt acagaaacgt caaagattgt ttatgcagat
     3541 aatacagaga tatcaaaaat ccagacagat ctgatgcgca cgactatttc atctgataaa
     3601 atttcacctt tattgaaaac tgcaattatc agcacagagg atgaatactt cgataaacac
     3661 caaggttacg ttcctaaagc tgttttacga gcattagtat ctgaagctac tggtattggc
     3721 tcttctggtg gatcaacttt gacacagcaa ttagtcaaac agcagatcct tacagatgaa
     3781 acaacattca aacgtaaagc aaacgagatc attctttctg cacaagtaga aaaatacttt
     3841 tcaaaagatg agatcattgc tacgtatttg aatgtctcac catttggtcg taataataag
     3901 ggacaaaaca ttgctggagt ccaggaagct gcaagaggaa tctttggtgt agatgcaaaa
     3961 gatgtgacgc tgccgcaagc tgcttatatc gctggattac cccaaagccc gatcacgtat
     4021 agtccataca cgaacacagg tgcgctaaaa gaagatcttt ctgccggcct tgccagaaaa
     4081 gattttgttt tattcagtat gtatcgtgaa ggccaaatta cgaaagaaca atacgaagaa
     4141 gcaaaagcct atgaccttac aaaagacttt ctaccacaac aaatcgcaga acagactgac
     4201 agagaattct tgtattatac tgtgatgaat gaagcaacaa gaatcattgc acaacaattg
     4261 gctgaaaaag acaacgctga tatgtctgat tcatcggtca gtgacgctta ttatcaaaag
     4321 gcacagcaaa cgatccaaaa taaaggatat acaatccatt cgacaatcga taaagatgtc
     4381 tatgctgcat tgcaaaatgg cgtagaaaat tatggataca tgttagatga tggtgctggc
     4441 agtcaagtag aaaccggaaa tgtattgatg gataacagaa ctggccgcat ctacggattc
     4501 gttggcggac ggaattacag tcagaatcaa aataaccatg ccttcgatac agaacgtcaa
     4561 gctggctcat caatcaaacc agttcttgtt tatggacctg caatcgacat ggggcttatc
     4621 ggatcagaat cccgtgtatc agattatgca acaacttggc aagaaggaga aaatgctggc
     4681 gagaaaatcg tcaatgctac taacgaagga tcgaacacct tccaaaccgt ccgtgagtca
     4741 ctagaatggt ccaacaatat cccggcttat cacttatatc aagatgtgtt gaattccggt
     4801 ggatcgaaac aatatgctta cgagcactac ttggcaaaga tgaactaccc ggcaaatgac
     4861 aattggggcg ttgaatctgc tcctttaggt acagtagatg tcacgacgct tcaacagacc
     4921 aatggatttc aggctttagc aaacggcggc gtctatgaag aaggctatat catcgattcc
     4981 atcacagata acgcaggaaa tgtaatttat aaacacgaaa gcaactctgt ccggatctat
     5041 tcggaagcaa cggcatcgat catgaatgac atgatgcgca gtgtgatcaa tgcaaaaatc
     5101 actacaccat tcaaagatgc catctcaagc ttaaacggaa atctaggaaa agcagattgg
     5161 gtcggaaaaa ctggttctac caatgaatac cgagattcat ggctaattgt ctctactccg
     5221 tctattacca tcagtagctg ggcagggcac gatgacaata ctggaatgga cagcaaagca
     5281 aggatacgtt cggctaatta tcttgccaat ctgatcaatc aagcttacca agcaaaacct
     5341 gacatttttg gcactgatga gaaattcgaa ttatcatctg atgtgatgaa gaaaaaagta
     5401 tctggattta ctggtcaaac acctggaaaa gttactgtca ataaaaaatc catccaaaca
     5461 ccaggaaaaa cagttgaatc gctatgggcg aaaaatggac cgaagaaaag cgcctttaaa
     5521 tttggcgttg gcggtaccga tgaaaactat acagattact ggaacaccgc ttcagcttat
     5581 gcaagagcga atcaagcaac caaagaatca gatgagaaag atgattgatt taatagaata
     5641 cacagtaata agggactgtg acaatagtat tgtcacagtc ccttattttt ttgatggtgg
     5701 tgaacataat gcagcatcta tacacggatt gttttatata cgataaatgt aatttcaacc
     5761 actttctctc ttttttaaga caaaaaaaaa ggattaaaca aaattttgtc ataacccctt
     5821 ttcccgaata aacggtgttc tcctgccaac tccgaatcac gagtcacaat attcatcaaa
     5881 catgaaatgc tgttttttga aattgctcct tgtaacgcag agttaaacgg caggctcaca
     5941 acctctgaat ggttgtcctt ttattatttt gtagatcctt tttcttcgat tccataaggc
     6001 aagataatcg ttttttcttc gacttcttct ttattcatca ttttcgtcaa taggcgcata
     6061 gataccgccc ctaaatcgta taatggttgt gtgatacttg ttaaacgagg acgagctact
     6121 tctgtcagta gtgaattgtt acttgtgatg atttcgaatt cttccggtac tttcacgcca
     6181 cggtctaata aaccatccaa aataccgatg gctaattcat catctgaaac atagacagct
     6241 gttgcaccgc tattatggat ccgatcgact agagatagac catctttgaa gttgtattgt
     6301 gcttcaaaga tcaatccttc gctatatggt aggttatttt cgcttaatgc ttctttatag
     6361 cctttcagac gattttgtcc attgatagga tcgatcaacg caccactgac aaaagcgatt
     6421 tttttattgc cgctttttgc caatttgttt actgcatctt ttgttgctga tacataatca
     6481 atgtttacgc tacctacttg ttcatctgga tcaatagagc cagctaatac aacaggagtt
     6541 tttgaacgtg aaaattctcc acggatttcg tctgtgatat ggtgacccat gaagatcacg
     6601 ccgtccactt gcttagccag taagttattc aatacattga cttctttttg gtcgttacca
     6661 tctgaatttg ctaagataat attgtatttg tacattgttg ccacatcatc gataccacgt
     6721 gctaaagaag caaagaacat attgctgaca tcagggatga ttaccccaac tgttgttgtt
     6781 ttcttacttg ccaagccacg cgcaactgcg tttggacgat aatccaagcg atcgataact
     6841 tccaagactt tttttcttgt tgctggtttg acgtttggat tgccatttac gacacgtgaa
     6901 acagtagcca tcgaaacatt tgcctctctc gctacatcat agatagtgat tgtttgcttt
     6961 tccatttgtg ttctcctatt catattttat atgttttctg aaaatacgtc ttacatttct
     7021 aaaatagaat atcaaataat ctcaagaatt gcaaccgttt tatctctgtt ttcatgaaaa
     7081 tttttacttt tttcagtttt tagaacg
//
LOCUS       DAYYKN010000138         7038 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000138 DAYYKN010000000
VERSION     DAYYKN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.140"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            350..946
                     /locus_tag="ACHBB7_002457"
     CDS             350..946
                     /locus_tag="ACHBB7_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9210355.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(1004..1486)
                     /locus_tag="ACHBB7_002458"
     CDS             complement(1004..1486)
                     /locus_tag="ACHBB7_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210356.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1592..1789)
                     /locus_tag="ACHBB7_002459"
     CDS             complement(1592..1789)
                     /locus_tag="ACHBB7_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210357.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2084..2842
                     /locus_tag="ACHBB7_002460"
     CDS             2084..2842
                     /locus_tag="ACHBB7_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM9210358.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2902..3330
                     /locus_tag="ACHBB7_002461"
     CDS             2902..3330
                     /locus_tag="ACHBB7_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210359.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3376..3448
                     /locus_tag="ACHBB7_002462"
     tRNA            3376..3448
                     /locus_tag="ACHBB7_002462"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3409..3411,aa:Gln,seq:ctg)
     gene            complement(3598..3969)
                     /locus_tag="ACHBB7_002463"
     CDS             complement(3598..3969)
                     /locus_tag="ACHBB7_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9210360.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4184..4369
                     /locus_tag="ACHBB7_002464"
     CDS             4184..4369
                     /locus_tag="ACHBB7_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210361.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4452..4640)
                     /locus_tag="ACHBB7_002465"
     CDS             complement(4452..4640)
                     /locus_tag="ACHBB7_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210362.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4664..4927)
                     /locus_tag="ACHBB7_002466"
     CDS             complement(4664..4927)
                     /locus_tag="ACHBB7_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210363.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            5085..5342
                     /locus_tag="ACHBB7_002467"
     CDS             5085..5342
                     /locus_tag="ACHBB7_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM9210364.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5413..5604)
                     /locus_tag="ACHBB7_002468"
     CDS             complement(5413..5604)
                     /locus_tag="ACHBB7_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210365.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5745..6065)
                     /locus_tag="ACHBB7_002469"
     CDS             complement(5745..6065)
                     /locus_tag="ACHBB7_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210366.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(6088..6522)
                     /locus_tag="ACHBB7_002470"
     CDS             complement(6088..6522)
                     /locus_tag="ACHBB7_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9210367.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6857..>7038
                     /locus_tag="ACHBB7_002471"
     CDS             6857..>7038
                     /locus_tag="ACHBB7_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9210368.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2216 a         1368 c         1071 g         2383 t
ORIGIN      
        1 atacatcttt accgaaaaat ttaactaaaa aacttattta agaaatataa actaattttc
       61 tataaaagca agtaattttt aatggcaata tcataaaaaa aaagaagata gagatttaat
      121 aattatatat cagctgtttt tttcaaataa gcaaaaattc ctaaaacaca acaatgaaag
      181 cgataactaa gaaaatcctt acataacgat ttttcttatt aaaattgttt tttgaacttc
      241 gcttattttt tgtgagcata tctgtttact tactttttgt aaggtgctat acttcatttc
      301 gtaatcaatt aaaaaaatgc aaaacgcaaa tcaaaggaga aattttatta tgacaacttt
      361 tactgacact ttgaaaaacc gccgttcaat ctatcatcta ggacgtaatg tttcactttc
      421 taatgaagaa ttaacaactt tgatcaaaga agcaattaaa gaaagcccaa ctgctttcaa
      481 tgctcaatca acacgcgcag ttatcctttt cggagatgca catgaaaaac tttgggaaat
      541 cactgaagaa gcacttcgcc cattgactcc agcagaagct ttcccaaata cacaaaacaa
      601 attagctggt ttcaaaaatg gttatggtac agtattattc ttcaaagata ctgacgtggt
      661 aaaaggtcta caagaacaat ttgaattata tgcagacaac ttcccagatt ggtcagaaca
      721 atcaaacgga atcgcaacag ctaatacttg ggtagcatta gttgataaag gattgggcgc
      781 aaacttgcaa cattacaacc cagttattga cgaagcagtt gcaaaagaat ggaatatccc
      841 ttcaaactgg aaactacgtt cgcaattagt attcggttca ccagaaacac cggctgagga
      901 aaaagaatac atgaacgatg ccgatcgctt ccgcgtattt ggataatttt atttgaaata
      961 aaaaacagag gttgtgacaa acatcttgtc gcaacctctg tttttattct ggatcaatca
     1021 taatatcaat ctctgcctca tcgtttaagc gttgggacaa aaatggaact aattcttcta
     1081 atgatgcaga aaatttcagc atcggtcctc gccatccgtt tcggaaaaaa ttcattttta
     1141 cgattttaga accttttggc tgatataata caattttatt gatctcagaa aaaggaacac
     1201 cttttttgtc aaagctattc aaaacaagtc cttcttctgt gattcctcta ctgtccaaca
     1261 aaaaactaat aaaaatcatt gctgacaaga tcccttttgc caattcatct gctgtaggat
     1321 gactgatgag tgtataccaa atgactagtg acactaagaa gggtatcacc aagtagaccc
     1381 atcgatttcg cacagtccga taaataattt cttttcgaac cagccaaaga aataaaaaca
     1441 tcacaacaac gaataaaaac aatagaaccg taaagaatgt taccatgaca actcgcctcg
     1501 ctttttcttc tccagaaaat aggaaaagaa tactgattta ccttctcaaa gaaattggac
     1561 ttgcagcacg ccgcacgtcc aattgttttc tttattcaaa tgtatcatat tttgcaacaa
     1621 ccatgcttgc tttttcatct aataattctt ggtcctctgc agcacattgg gcaactttga
     1681 ccacggcgca gtttgtcatt tttgatacaa cttcaccaat gaccgttttt gtaaaaccaa
     1741 ttggtttgca tgcatattgt tctcctaaat taatcatttc ttccgtcatc aaaatcctcc
     1801 taaactgctg gaacttttct ccacattacc actgctattt ctttggagat ttaactcatt
     1861 gatttttatt tacagttaaa aatcaacctt acaggcatta tagcgtactg ttttcgtttt
     1921 cacaattaaa acagctgttg tatttagaag aatttttttc aaaaaattaa aacatattcc
     1981 ttaaaaacta attatttcct acatattaaa atataatact tccatttcca ataaaaatac
     2041 atttataata ggaaagataa acagctgaaa agggagattt caaatgaaga aaaaaacgtt
     2101 caaaaaacta ttgtttctag ggggcacagc tgctctttta ttaagcggta caagttatta
     2161 cctacatatc cattctccag aacatacaat gaacactgca caaaaaagtt caacacaaaa
     2221 aacgaaaaag acaaaagaga aagcagaagt tttactagat gttccattag agagccagtt
     2281 tgattctcct tctttagaaa atggctgtga agtcacttcg ctttctatgc ttttatcttt
     2341 ttatggttat gaaacaacta aaaaccagtt agctgagcaa ttggattacg tccctgtttt
     2401 caatgcagat gggactcatg gcgatcctaa tgaaggattt gttggtgata tcagtggcgg
     2461 agactgggct atggtcgtct atgttccccc agttgcctcc cttgctcagc aaatcgtcca
     2521 aacggattat cacacttttc caaaaaccga tgctcaactc gatgatatca aaaaagcact
     2581 atctaaagga aatcctgtct ggacttctgt gactatcaat tttgaagtcc cagatgaaac
     2641 tgattttatg acttggacca caaataatgg agaagtaaga gtcactcctc ttgttcatgc
     2701 ttgtgtagtc actggatatg acaaagaaaa tatttatgtc aacgatccct atggtgtaaa
     2761 aaatcgagca gtaccgatca acgatttttc ggcgattttc actgcaatgg gtggacagat
     2821 gctaactttg gagaaaagct agtaattcaa acaaaaatcc cttatactca agataccaaa
     2881 cctttgaagg gagcgtagat aatggaaaaa ttcacacata aaaaaatgga tccaaatgaa
     2941 attccgatta tttttgtccg tgactgcaag ggaaatgttc aaggtaaagt ctctattaat
     3001 gagtggaacg agcgtcgcag acctgctacc ctcaatgaac tggaaatcaa attgtatcgt
     3061 caatctcttg tttattacgc agatcaagag tacgagaaag ccaccgattt attgaaattt
     3121 ctaattgctc gaacagagta tactcgcttt gaatatattg aacgtttagc aaacatctat
     3181 catattatga atgaacctgt caaagaatat cagttattgg acaccgtctt gtcagtagca
     3241 gaactgattg cccttcctgc tggattagaa aagaaactag tacgccgcct tttacgagta
     3301 aagcaacaac tatccgatca agaaaaataa cttgcgtttt tttaggaaag tgctatgatt
     3361 attttgttga ataaatggga tgtagccaaa ctggtaaggc acttgactct gaatcaagca
     3421 attgtaggtt cgaaccctgc catcccaatt tgatgtgctt gctgaaaaaa tgaaccccct
     3481 gtaaaagaac aatctttttg cagggggatt attgtattat ttttactact tgtatttagt
     3541 ttacataatt aaattatgaa taataaaatc gggcgtattc ttaatacaaa aactgattta
     3601 cccaataatt gctaatgctt tctccggaac tttttctcta ggaacttcct catacgtttc
     3661 tatgtgtgct cctttattgc taattttcaa ataatgattt tcttttaaca cagaaagacc
     3721 attaaattca accgttcgtt ctttcccatt ggcatcagca gctttctgca cataccttgc
     3781 tgtaccatat tcatttactt cctctggttt ttgtgtcttt acatagacct ctcctttttc
     3841 aactaatgga tttagctgat caagtatacc agggatttct ccataacttc cttcagtata
     3901 atatttcagt ccaaatagca aaataccact aatcgccgcc agtccaatca aatatttaat
     3961 cactttcatc tttctacctc cttgttatat attcagtata tgggtcagtt aaagaaattc
     4021 catccgtccc aagttggaga tttttctcct actttagaag cgagaatcga gtgttttttt
     4081 agtaaaaatg atcgttattt gatattattg acgtgtggat aaatcagttt ttctcgtttt
     4141 gtccatgtaa aaaatcatgt tgatagcaaa ggatgtgttc aaaatgaatc aagaagaatt
     4201 atttcaaaaa gtcaaggaaa tgattaagaa cggcaatttc gatggcgcaa aacgatttat
     4261 cgaagaacat aaagaacaat taggacctta taaagagaaa gcccaaaatc ttttaaaaga
     4321 tgtaaatatc gacagtgtca aaaacaaatt taaaaatcta ttcaaataag taaaactttc
     4381 acttttgtaa aatcaaagga ggacaatgct gctgcatcgt cctcctttgg ttcttttctg
     4441 aatacttcat actaaaataa acgtgtcgga tcgtaatgat aagtcacatt gccgttcagt
     4501 gaggcttttc ccaccacata tttacgattc tccaacttca gccacgcttc tcgaaaagcc
     4561 attaattctt ctctttttac atcaatcttt tcgatttttc catcgatcaa atcttgaata
     4621 agttgttcat atggattcat ttttttcctc catctgctgt caatcaataa gttgtaaaaa
     4681 taggttcata tgcttttttg gtatctagat cttctctgac tttgactgtc gtataaataa
     4741 ttttttctac tgggatacta taataacttc cttcaccatt attgaatcgg tgaacgtctt
     4801 tctttgctaa aataccattt attttattcg tcagtacacg ttgttcgaca ctgttactta
     4861 caacatagta aatcacccga atattattga tcaaatgcaa atcgatttct aacactacat
     4921 gatccatgaa aatcccactc cttctttcac tttgattata gcaagtttct cctctaattt
     4981 caaaatcgat acagatttta ccggataaat ccacaaaact gcttgcgcca tttcgcacaa
     5041 agtgttattt tgtatcatgg actttattta tcggaggcaa tagtatgatc gcgatcggtc
     5101 aatttgtttt ttatattcct ttttttatta tgataagcat tcttttctat tacataaaat
     5161 ggacaaaaaa gaagttttca gttttactgg cctctttacc tgctgtatat tttacttatc
     5221 aaattttttc ttttaggcat tgggaaacaa cgtctgtatt aataacacac atcatcgaat
     5281 tgacgctttc agtaattttt cttattattt ggatctattt tttatataag aatcagaatt
     5341 gaatccctcg catgtttaaa aagaaaacga ttaaagatgc agcaaatttc tgccgatctt
     5401 taatcgtttt ttctattctg ttataccaaa aaaatgtttg aatcggtgaa ggatcgttcc
     5461 ttgttttaat tctataaatt cttgtgcgat cgggtacttt tcttccaaat tttctttgtt
     5521 cttttcgatc aggcttttcg cttcttcata ttttttatct gcacatagtg cttttacttc
     5581 ctgtaatact tttcctgctt ccatttgcct caccctcttg ccattttatt gctttaagtt
     5641 ttatttaagt tatacgcatt attctagttt catcccaaat aacctttttt catttttact
     5701 cctcgaacta atcagttttc taaaggctga ttggttcgtc ttttttattt ttcctccatt
     5761 tcattctgct cttctgttaa tttatctaat gctttgattg atgctgcggc tcttgcataa
     5821 tcatagctgt ataattccat aaatttatca ggcagaacaa accacgctgg atgataaccg
     5881 ttagctgtga cagggaatcc tttatcttgt tggttatctc catcccatct cagagcaaca
     5941 cctttttttc ctttccactt tactaaagcg atcgagtatc ccttctttgt ttgtgtgccg
     6001 tcttcaataa ttgctaaaat ttctattttt tcttttggcg cacggacttc ttgaggacga
     6061 accattttct attcctccct tatctcatta cttcacgacc aacacactgc aaggggcatg
     6121 tgatacgaca taagaagcgg tcgatccaac taatgcttgt tgtatagcac cttttcctgt
     6181 cgctcccata acgatcaaat caatattttc ttgtccaggg atgacattgg caatcaaatt
     6241 tcttggatct ccagtttcaa ctattgcgtg gatctcagca atgccgtact ctttggcatc
     6301 attgattttt ttcagcatgg ctacttctgt tctttgtttt tcatctgcat agatttttga
     6361 aaaggcatag gcactatggg tcagctcaac tttgttgatg attgacacga taaataagtg
     6421 ggcttcattg tcctttgcta agtcaagggc ttcagcgaac gctttgatcg attgtcttga
     6481 tccgtcaact gctaccatga tgttctggta tcttttactc attggaaccg cctccttttt
     6541 cttttattgt aacatctcaa gcaaaaatac aaaaaatata cgcaatatgt aaataaaatg
     6601 atgaacaaaa gcaatttctt tcaagttatt tatacatttc ttttctcatc agaacacctg
     6661 ttctaatccc attatttctg cattatgtga aattcctcat catttttcac atcgagcgaa
     6721 catcattctg ttacaaaaaa cgtggatttg tcataaattg tcatgcttct tttacctatg
     6781 aaatattttc acgtgttaaa gtattcctcg ttgaacacaa caaataaaac tttttaacga
     6841 ataaaggaga tttttcatga catcacttaa aactttactt tttggaacaa ctttggctgc
     6901 cggcgctgca ttcttcatgg gaacgactgc tcatgcggac gaagcttata ccgttcaatc
     6961 aggtgatact ctatcaacga tttctcaaaa atacgttggt gataactcct tgatcaatgc
     7021 cattgcagaa tcaaattc
//
LOCUS       DAYYKN010000139         6881 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000139 DAYYKN010000000
VERSION     DAYYKN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.141"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..765)
                     /locus_tag="ACHBB7_002472"
     CDS             complement(13..765)
                     /locus_tag="ACHBB7_002472"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9210369.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1129..3795)
                     /locus_tag="ACHBB7_002473"
     CDS             complement(1129..3795)
                     /locus_tag="ACHBB7_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9210370.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4136..5587
                     /gene="cls"
                     /locus_tag="ACHBB7_002474"
     CDS             4136..5587
                     /gene="cls"
                     /locus_tag="ACHBB7_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM9210371.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(5916..6731)
                     /gene="thiD"
                     /locus_tag="ACHBB7_002475"
     CDS             complement(5916..6731)
                     /gene="thiD"
                     /locus_tag="ACHBB7_002475"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM9210372.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         1962 a         1464 c         1190 g         2265 t
ORIGIN      
        1 aaaaagaacc cttcacgagt cgtgaaaggt tcttttttta tctgcggacc aaaggagcag
       61 tctataggtc agcttagtcg tactaaggat ttctcgtttt tcactgaata gtttgcgaat
      121 gtacctggtc acaacaggat cttttgcgta cgcgtcatag tatagcttca tcaattcttc
      181 agtaattgat tcctgtgtaa aataagtaaa atcttttttc ttcactttga atccttcatc
      241 aattaagtat gtttcaaatt gatccattac aactggcgat gtattaggat cttcttctat
      301 cattcctagt tgcttttcga taggataaaa aacatcatcg attgattcca ctactctgat
      361 tatcacgcac catttgcttg actttttcat caatttttct aattgaataa gatgtaccaa
      421 tgctggattt gctgctgtaa aaaccatttc atatgtctca tttgattgga taaattcttc
      481 taacgactgt ttgataaagg aaacatttgg caactcattt ttttctgctt ctttttttgc
      541 attttctagc attcctgaag aaaaatcgat caaatcatag cgattaattt cttttgctaa
      601 ggaaagaaat ctgccagatc ctccagcaac gtctgctgct gtttcagcag gcaggacttt
      661 ctccgttttc agataatttt ttacatcttc agcaatagtt acttgcgatt cttgctggat
      721 ttcataatat tcttcagcaa attcatccca ttctttttca ttcatttgat ttcctctaat
      781 tttttgttat atgttttcac ttttcggaat aaatcattct cctcttttat ctattctatc
      841 tcttttgatg aataataaaa tgattggctt tccgtctatc ataaataaaa aaagggactg
      901 tgaccaaagt cgtgtcaaag tccctctttc cgaataaaca gtgtccaatc agatgaccct
      961 cagtattagc tcaaaatgct gattttacaa cttcttcctt aaacggtgtt ctcctgccaa
     1021 ctcttcggtc acaagtcaca atattcaaca aacatgagaa gctgtttgtt gaaattgcac
     1081 cttgtgactc agaattaaac ggcaggctca caacctcgtt gattcgtttt atttatttcc
     1141 ttgtactcgt acaatgatca atttagtgat ttccatcaaa acaattgtca caagtgcgat
     1201 tcccatacat gtaaagactt ctgtcaatcc aaatgctgat gggatagaga agacttcttt
     1261 cagtcctggg atcattgtta agcagtaaag cgcgctacat acaactacag ctagtaagac
     1321 acttctgtta gagaagaacc ctgcttggat agctgtttgt gtgtttgaac gtgctgggaa
     1381 tgtttgcaat gtccgacacc aaatcaatgt tgaaaatgac atggctacac cgaattcagg
     1441 tgaatgctgc atgccaagcc attgagcaaa gataacagca atcccaatca gcacaccacg
     1501 atacgttaca gaaatcaatg ttttgtccgt aaagattcct gaatttggat ctcttggcgg
     1561 gcggttcatc gtagcaggtt cagctttttc tgtacctaat gcaattgctg gcagtgaatc
     1621 attgactaaa ttgatgaaca acagttgtaa tgctgtaaat ggattggacc agtcagcaac
     1681 taaggcaaag atgatcgcaa tgatcgcacc taaatttcct gcaaatagat aagaaatagc
     1741 tttcttgata ttatcaaaca cattacgtcc tacttctact gcgctgacaa tagaagcaaa
     1801 gttatcatcc gtcaatacca tcgctgctgc atcttttgcc acatctgtac ccgttcccat
     1861 agcaattccg atgtctgctt gtttcaatgc tggtgcgtca tttacaccat cacctgtcat
     1921 agctgagata tgacctttat tttgccaagc acgaacgata cgaattttat tctcaggtga
     1981 aacacgtgca taaacagtga ctttttctaa aacttcgttt agttgatctt ctgtcaattc
     2041 atctagttcc gtcccggtca aagccaggtt tccttcttca aaaatcccga tttgttttgc
     2101 gatagcaaca gctgttgttt tgtggtcacc agtaatcatg attgttttga ttcctgcact
     2161 tcttgcttct gttaccgcct gagtcacttc agcacgtgga ggatcgatca tagcaagcaa
     2221 accaacaagg atcaaatcgt cttcatcttc aaatgataaa tcaaaattat caagtggacg
     2281 gtaagcaaaa gctagtacac gtaatgcgcg tgttgaaaaa gcttcatttt gctcttggaa
     2341 ttttttcatc aattcatccg tcatcggaac gacttcacca tttagcaata cttttttact
     2401 gcgttggaag acgatatctg gtccgccttt aacaaacaag taatttttgt tatcaattgt
     2461 gtgtccagta gacattaatt ttcgatctga gtcaaaagga agttctgctt gtcttgggta
     2521 gttgtttcgg atttcctgat aaggctggtt taatttttca ctaaagtcga taaacgctac
     2581 ttccgtaggg tcgcccaatt tagtaccgtc ttcactgatc gatgcatcat tcgctaaaac
     2641 actgatttcg atcaagcgac gttcgtcagc tgtccatttt gaagggtcat ttgtgaaatc
     2701 cccagtatcg ccattaccta aaaagtagtc aacaactgtc attttgtttt gtgtcaatgt
     2761 accagtctta tctgtacaga tgatacttgt cgaaccaagt gtttcaactg ctggaagctt
     2821 ccgaataatg gcatgacgtt ttgccatttt attcgttccc aaagataaca cgattgttac
     2881 gatagactgt aatgcttctg gaatagcagc tacggctacg gctacggcaa acatcactgc
     2941 gctgacgata tctgcagtca tatctcctgt accttctcca agataaatac gaaacagttg
     3001 gatacctaaa atcaataaag acaatgccaa aatagccaaa cttagttttt tactgaattt
     3061 atccagacct cgttgtaacg gagtgacttg ctcagttgtt gattccaaaa ggccagcaac
     3121 ttgtccgatc tccgtattat tcccagttga agtcactaag aatttaccgc gtccgtaagt
     3181 tacgatcgtt ccactgaata ccatatttgt acgatctccg attggtaccg tacttgtgat
     3241 atccgttact tctttttctg cagaagtcga ctctcctgtc agcatacctt catctacttt
     3301 taatgatccc gcatctagta aacgtccatc agcaggtaca taatcgcctg cgtctaacaa
     3361 cacgatatct cctaccacaa gttcgtttgc cggaatcgtt ttttccttac catcacgaat
     3421 cactcgagca tcgggcgctg aaaggttttt caacgcttct aaagaacctt ctgctttttt
     3481 tgtttggaca acactgatta tagaattcaa catcaatact gcaaaaataa ctaagaattc
     3541 gatgtagtca cccatgacca tctgaatcag tgcgacaatc agcaatacga taaccattgc
     3601 atctttgaaa gtttctaaga acaatttcca agttggcttc ttcggctttt cttttagctg
     3661 gttcaagcca tctctttcta atttttcctt cgcttgcgtg gtgctcaatc catcggttga
     3721 gctctgcgtt tgctgcatca acgagtcgat cgtttcttta tagaccgctt gttgtgcttg
     3781 ttccttgttt tccaaatttc atcccccatt ttttgatctt ggccctagtc ctgtctgaaa
     3841 aaaaagagac ctaagacaaa tacgcatttg tcttaggtct caccatttca tacaatacca
     3901 gaaaaccatt tggttaactt actgttgata ttatcgcaac aaacgttgcc ggttactccc
     3961 ctaaggagat attcaactgt actaatatcc taccttatta acctacaaaa agcaactact
     4021 ttagcccgat ttacctacat tttcatgaaa ggagtgaaaa gtatattcac cttggtaggc
     4081 ttctttcttc tatcatgcta tactaatgtt attgtatgta aggaggaaca tttttgtggt
     4141 atctagtatt ataaccgccc tttatctatt aaatgcactt attgctttgg tggctatttt
     4201 gatcaaaccc cgagatgtag cagccatttg ggcatggctc ttagtattgt ttgcccttcc
     4261 tggggtgggc tttgtattat atttattttt cggacgtgga ttaacggata agaaaaaatt
     4321 ctatctccga caaagtgact tgaaagaatt agaaaacttt cagtctttta gaggagatac
     4381 cattgaacat tacgatcctg acatgggcga taaagacaaa cagcaatttg ttgacttctt
     4441 ctcttcatta aatcgtatgc cgctgacaag aatgaattct gtcactcttc tcacagacgg
     4501 acaagagaaa ttggattcac tgcttcaaga tctaaaaaaa gccaaacatt cgatccatat
     4561 cgaatattac gcatttgtga cagataatat cggccagcaa gtcttacatg ttttagaaga
     4621 aaaagccgca gaaggcgtgg aagttcgaat attatatgat gcatttggct ctcatggcac
     4681 aaaagcaaaa gatttcaatc gtctaatcaa aaatggtgga catgtccata catttgttac
     4741 ctcacaaagg gcattacttc gtttccgatt gaattaccat gatcaccgaa aaatcgttgt
     4801 gatcgatgga aagattagtt ataccggtgg tttcaatatt gccaatcaat atgtaaatac
     4861 aacaaaaaaa ttcggctatt ggcgcgatac gcatatacgg attttcggtg ccgcttcttc
     4921 tttgctccag cttcgcttct taacagactg gaacgtctcg gtacctgaag aaaaaaaggt
     4981 cggctatcat ttgaattatt tctttaaaaa agcagatcga gatgaatcta agcttgctga
     5041 tacatccatc cagcttgttt caagcggacc gaataacgaa agggaacaaa tcaagctttc
     5101 atttatcaaa ttgattactt ctgctaaaaa acgtgtttgg atacagacac cttaccttgt
     5161 tcctgatgaa agtgtcattg ctgctttaaa aatcgcaact gcctctggtg tagatgtgaa
     5221 aatcatgatt cccaacaaac cggatcatcc ttttatttat cgagcaacac aatattatgc
     5281 tcggcagctg atcaaggaaa atgtacaaat ccttgtctat gagaacggct tcctccatgc
     5341 aaaaacattg ataatggatg atgaaatctg catggtaggt tcagcaaatc aagatattcg
     5401 tagctaccga ttgaattttg aaacaagtgc tgtcatttac gatcctgagt ttttagaaga
     5461 acttgctact cagttcaaag aagatgagac acattgttca tccatgacaa ctgaaacagt
     5521 caaggaaatg tctaactggc tattattcaa gcaacaaatt tctcgattat tttctccaat
     5581 cctataattt ttttattcaa gtagagaaaa atatactaag attaaaggga ctgtgacaaa
     5641 aatcttgtca cagtcccttc ttccgaacaa acggtgttct cctgccaact cctcggtcac
     5701 aagtcacaat attcaacaaa catgagaagc tgttttttga acttgttcct tgtgactcgg
     5761 agctggacgg caggctcacg acctctttct taaacggtgt tcaaaagctt gcatctgcgg
     5821 taataagtcc aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac
     5881 ttgatgcagg gcaacttttt cacaacttct ttcttttact ctaattcacc agctgcccaa
     5941 tgattgatgg ggccatattt gtgccctaca tttatttctt cacgaattgc acggtttaca
     6001 tagtcttttg ccaatcggat cgcttgttca actgtttttc ctttcgctaa ctcagctgca
     6061 atacatgcag atagcgaatc gccagttccg ttgatccggt cggtaggata aaatggtgca
     6121 tttaaccaaa atgattttcc tgattcgaac aatacgtagt cattgactgt ttcttgctgc
     6181 gcatttttag catgatgtcc tttgatcata acgttttttg cacccatatc ttgcagttct
     6241 tttgctgctt gcacaaaatc ttcttcagct gctaatgtcc gtccagtcag cttttctgct
     6301 tcataaaaat taggtgtcag tacttcggcc aaagggacaa gcttttcttt caagctttgc
     6361 aaagcagatt cttctagtaa aagatttcca tgtttcgtta cgattacggg atccacgact
     6421 aatggtccaa aatcatattt ttggtaattt tttacaactg tctcaatcaa ctttgaatct
     6481 gctagcatac ctgttttagc tgcacgtatt tgaaagtcct tcgcaatcag ttcaaattct
     6541 ttgtcgataa aatctgttgg caatgtcaca cttgctccga ttccgtaaga attacctgca
     6601 acacatgcgg tcatgacaga aacaccgtaa acatttcttg taaagaatgt atgcatatct
     6661 gcttgcatcc ctgcgcttcc atcgctatct gatcctgcga tcgtcattgc ttgtacatac
     6721 tgatcttcca tcaaatcgat tcctccattt ttataaaaag atttttcata atactgttac
     6781 tgtttctatc atatgttttt caaagtatcc ctcagtaaat ataccactac cttttcttgg
     6841 taatcaaatt tatttgaaaa aacatttgca ataaatcaga a
//
LOCUS       DAYYKN010000140         6686 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000140 DAYYKN010000000
VERSION     DAYYKN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.142"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACHBB7_002476"
     CDS             <1..608
                     /locus_tag="ACHBB7_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210373.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="ACHBB7_002477"
     CDS             628..1656
                     /locus_tag="ACHBB7_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM9210374.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="ACHBB7_002478"
     CDS             1653..2411
                     /locus_tag="ACHBB7_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9210375.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="ACHBB7_002479"
     CDS             complement(2497..3237)
                     /locus_tag="ACHBB7_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9210376.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="ACHBB7_002480"
     CDS             3298..4551
                     /locus_tag="ACHBB7_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM9210377.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4846..5100
                     /locus_tag="ACHBB7_002481"
                     /pseudo
     CDS             4846..5100
                     /locus_tag="ACHBB7_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="ACHBB7_002482"
     CDS             5222..5548
                     /locus_tag="ACHBB7_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM9210378.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="ACHBB7_002483"
     CDS             5616..6329
                     /locus_tag="ACHBB7_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM9210379.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
BASE COUNT         2343 a         1109 c         1318 g         1916 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaat
//
LOCUS       DAYYKN010000141         6335 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000141 DAYYKN010000000
VERSION     DAYYKN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.143"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACHBB7_002484"
     CDS             complement(54..788)
                     /locus_tag="ACHBB7_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9210380.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACHBB7_002485"
     CDS             1115..1525
                     /locus_tag="ACHBB7_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM9210381.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACHBB7_002486"
     CDS             1518..2219
                     /locus_tag="ACHBB7_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM9210382.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACHBB7_002487"
     CDS             2219..3805
                     /locus_tag="ACHBB7_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210383.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACHBB7_002488"
     CDS             3824..4045
                     /locus_tag="ACHBB7_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM9210384.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACHBB7_002489"
     CDS             4042..4803
                     /locus_tag="ACHBB7_002489"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM9210385.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACHBB7_002490"
     CDS             5183..5692
                     /locus_tag="ACHBB7_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM9210386.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACHBB7_002491"
     CDS             complement(5767..6306)
                     /locus_tag="ACHBB7_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM9210387.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
BASE COUNT         1969 a         1168 c         1288 g         1910 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tattt
//
LOCUS       DAYYKN010000142         6280 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000142 DAYYKN010000000
VERSION     DAYYKN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.144"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..184)
                     /locus_tag="ACHBB7_002492"
     CDS             complement(<1..184)
                     /locus_tag="ACHBB7_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HFM9210388.1"
                     /translation="MNILILSCGRRCELVNAFKTALNNNEMEGKIIGADCSELAPALY
                     MCDEKIVLPKVSESNYI"
     gene            complement(196..2013)
                     /locus_tag="ACHBB7_002493"
     CDS             complement(196..2013)
                     /locus_tag="ACHBB7_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM9210389.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(2027..2791)
                     /locus_tag="ACHBB7_002494"
     CDS             complement(2027..2791)
                     /locus_tag="ACHBB7_002494"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM9210390.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            complement(2819..3517)
                     /locus_tag="ACHBB7_002495"
     CDS             complement(2819..3517)
                     /locus_tag="ACHBB7_002495"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM9210391.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3529..4308)
                     /locus_tag="ACHBB7_002496"
     CDS             complement(3529..4308)
                     /locus_tag="ACHBB7_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM9210392.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4324..5268)
                     /locus_tag="ACHBB7_002497"
     CDS             complement(4324..5268)
                     /locus_tag="ACHBB7_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9210393.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5314..6093)
                     /locus_tag="ACHBB7_002498"
     CDS             complement(5314..6093)
                     /locus_tag="ACHBB7_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210394.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1647 a         1308 c         1131 g         2194 t
ORIGIN      
        1 caatataatt actttctgaa actttaggta atactatttt ttcgtcacac atatatagtg
       61 cgggtgctag ttcagagcaa tctgctccaa tgatttttcc ttccatttca ttattgttta
      121 atgctgtttt aaatgcattc actagttcac aacgacgtcc acaacttaat attaaaatat
      181 tcaattttta atctcctatc tattcgaagc atttgcaaaa gtgacaatgt ctttagccaa
      241 ttgttcatcg tcttgcggca ggctcttgac aaaatccatc actttttgta ttgagtaacc
      301 tttgatgttt cctacaaaga ttttctcata aactgcttca tcatttcttt ccttatccaa
      361 caatagttct tcatatagtt tttcccctgg acggatacct gtttcaatga tttcgatttc
      421 gtcttcgcta tacccgctca atcggatcat gtttttcgct aggtctacaa tcttcactgg
      481 ttcactcata tctagaataa aaatttcgcc accttttgct aatgcgccag attgaataac
      541 taaacgacta gcttcaggaa tcgtcataaa gtatcgagtc atacgaaagt ctgttacagt
      601 gattggacca ccttgagcaa tttgttctct gaatactgga atcacactac cgcgagaacc
      661 aaggacattc ccaaatctga cagctgagaa tttcgtacag ccttcttcat tcaatccagt
      721 cacaatcatt tcagcgattc gttttgttga acccatgaca tttggaggat tgttagcttt
      781 atctgtcgat accatgacga agtttttcac ttttgctgct ttagctgctt ctgctacatt
      841 ttttgtaccg aaaatattgt ttttgactgc ttcttttgga ttatattcca tcagtggtac
      901 atgtttatga gcagctgcat ggtagacgat atctggatga tattgttcca tgatctcatt
      961 gattttctcg cgatcttgga tatctgcgat gatcggtacg atttctgtag gacaattttg
     1021 atgatgcgtg cgtaactcac ggtcaatcaa gtagatcgag ttctccccat gtcctaaaag
     1081 caatagcttt gcaggattga attgaatgat ttgccgacag atttctgaac cgatcgatcc
     1141 tcctgcgccg gtgacaagaa tgactttgtc tgtgatctga tctttgatgg attcaatatc
     1201 cagctttacc tcatctcttc ccagtaaatc cactacgtca attgttttta attttgatac
     1261 gctaattttc cctgaagcaa gttcttctat agaaggcatc gtgttgactt tcacacgagc
     1321 ggattcgact aactcgaaga ttcgtcgtaa tttttttctt gataaggaag ggatagcgat
     1381 cgttaccatt tgaatgtcat atttttcaat caattccgga atatctttta atgcacctaa
     1441 tacttttttt cctgaaagat acgtatttct tttatttgga tcatcatcta caaacccgac
     1501 aacgtggata tcttgagccg ttttggatcc aagaaagctg ttgtataaaa tacgcccgcc
     1561 ttcgccagca ccaacaatca atgtattttt agcagaatct gctgatacgt atctcatatt
     1621 ttttgtttcg atgtagatac gccagatcaa tcggctaccg atgatcaata agagcgaaag
     1681 cagataagca aagataacta accgtaaact gtatcgccga ttaatgaaca gcagtatgag
     1741 gatacttgag gcagcacttg cagatagtga actaaaaatt gcaatgatct ctcttaaatt
     1801 tgtatagcga ttgatccgag tgaaaacttt gaataaacta ccataaaata aataaaaacc
     1861 aatcgacaaa ccgatagaga ttaaaataag atccattggt atagatacaa acggcttcat
     1921 gaatttagct gctgcaatat ttgcagcaat taataacaag ctgtccacaa aaaccaatgt
     1981 ggcaattttt cttcttcttg ttaatataaa cactttttaa ttccccctaa aataaaccaa
     2041 acttcttctt ttttacctct tcatagtctg gataactgat ttcatccccg ttgatcagat
     2101 cctttgcaac ttgcttcatc tgtactactt tttccttacc aaagtccttg gcaatctgct
     2161 catagcactc ttttaagtaa aaggtgcgct tagagactcc gtgtgcatca gaagcagtca
     2221 tctgcacgag attatgtttg accattgttt tagctgtttt ctgaatatct ttgccaaata
     2281 ccccaacata acttggcgca gtaagttggg ccaaacaccc catatctagg aacggaagca
     2341 aatgattcgg gtcttcgcga aaatgcgcgt tgcgttctgg atggacgatc actggtgtct
     2401 ttcctagttc taacaactca aaaaaaagcc gttctgcata taacggcact tccagtgtcg
     2461 gaaattcgat cagaagatat gtatcatcta aatctgtaaa taaaatttca tctcggtgga
     2521 tgtcttcgat gagcgttccg gtaatacgta cttcttgtcc ttccaataac gtcaaaggca
     2581 actgtctttt ctctaattcg gcttgtaacg aagcgactag agaaatgact tgtgattttt
     2641 cattgctgta ttttccatta ttgtggtgtg gtgtgcataa aatatgcgtg atgccttgct
     2701 gaatcgcttt ttcagccatt gcaatacttg cttctaagtt ttctgccccg tcatcgattc
     2761 ctggtaggat atggcaatgt agatcgatca tttctcttgt ctcctctctt cttattcctt
     2821 aatttccgta gtaataacca gcatctttgc tatgttctgc tccgttgtat acaacaccta
     2881 aaactcgtgc ttgtaccatc tctagcagtt cttttgcttt attcaatgat tctttacgtg
     2941 atgtattttc acgtacgacc aataaagtgc catccgtttt tgaagacatg atctgtgcat
     3001 ctgttacggc tactactggt ggcatatcga atatcaccac gtcaaataac tgtcttgctt
     3061 cgtctaggat ctgatccatt cttggcgaac ttaacaattc tgatgggttg ggtggcttag
     3121 gtccactagg caaaatcgac aagttctcga ctggcgttcg ttggattaca tctgctacac
     3181 ttcctgatga actaagtgct gtacttaatc ccctggcatt gttcagctgg aatgtcttat
     3241 agacgacggg tttacgtaaa tctgcatcga ctagtagaac tcgttgaccg gatttagcaa
     3301 aaacgactgc aatgtttgct gcagtggtgg attttccttc tccaggacct gatgaagtca
     3361 cgacaatcgt tttgatttgc tgaccagctg cggaggcaaa ctggatattc gtccggatcg
     3421 tacgatattg ttcggcgatt ggtgaagatg ggtttgttaa tgtgatcaaa ctgactgcat
     3481 gagtttgtgc tgctttttgt ttctgtgttc gtgccatttt tcttcctcct agactcttga
     3541 gcggcttctg cgtgatgggg ctgttttatc gtttgtagct tttggtcgtg caggagctgt
     3601 tcctttttta cggataccag cgttcaattc tttcgctgac atttctggaa ctgttcctaa
     3661 gattgggaat cctaatgcat ccgtcacata tttttcatct ttcactgtcc gatcaagtaa
     3721 ttcgaataag aatgccagac ctacgcctac catcattcct aatactaagc caatcactag
     3781 attcaattta ttatttggcg aaacaggtgt cgtattggca actgcacctg aaataatcga
     3841 gattttatcg acattcatca cgtcttttgc gttctcttgg aaaacttgtg aagttgtgtt
     3901 agcgatctgt tgagcagtca ctgcattcgt gcttattgcc tggatcgaga acatttgtga
     3961 attttgtgac tgattgacgg agatcgcatc tttgatttgt ccagcagtca ttttcacatt
     4021 gtcttctgtt tctaaacgat ctttgacttc atttagtact aaatcactga cgatcatatc
     4081 tttatacgta ttgatcattt gcaggttcgt attgacatca ttgacatttg ctgtgtctga
     4141 ttgtggcagt gtcacgatca attgtgcttg tgaactgtat tttggtgtca agatgaaaaa
     4201 tgttacgatt cctgagatgg ccaagccagc aaacattgag atgatgattg ttaccaagtg
     4261 tttgcgtaat gtttcaaaaa tttctttcaa gctgattgtc tcttccattt tttctccact
     4321 ttcctattta ttctctagat tcatttgatc ttttaattct tttttcacac gatcaagttc
     4381 ttgatcgctg actttttgat aggagatatt atcttgcatg aagccttctc cttgcagctg
     4441 atcttgcttg atcgtaccaa atgcctcccg ataatctaaa gcgatttttt tcatttcatc
     4501 aaagctcatg tctgttttca tattcgactc tagagcattc aaaacggatt gatagttgct
     4561 gacaccgttt aaactcaata ctttttttgc tacaccttct acgatctttc tttgacgctc
     4621 ttgtctacca tagtcaccat ttggatcttc ataacgcata cgagaataag ataatgcctg
     4681 atccccgttc aatgtaattt taccgattgg aaagtcatag ccatcttgtg agaaagtgag
     4741 gtcattattg acttcgatac caccaactgc atcaacgagt tcctttagcc ctttcatatt
     4801 gatagagaca tagtgatcga ttgggatgtc tacatatttt tgtagggtat ccatcgccat
     4861 ggcagctccg ccgaatgcgt aagcgtggtt gatcttgtct tttgtgtcac gacctacgat
     4921 gtcgacatac gtatctcgag gaatgctgac gatcgttgtc tgtttgtctt caggactgac
     4981 tgtggcaacc atcatcgtat ctgatcttcc ttgatcgacc cgacctaaat ctccagtatc
     5041 aatgcccatc aatagaacag agaaagattc ttgtttgtcc agatcaactg gtttcgttcg
     5101 ttctccttgg gttcgttcca ctgattcgta agttttctca atagaatgtg agagatccag
     5161 atataatttt gctcctactc ctacaattgc taatactaat acgataagaa ctgctaaaat
     5221 aatcagaaat atttttttcc ctcttgattt tcttcttcgt cttgccatag tttcctccgt
     5281 ttgctaacct tgcttgaatc aacgttttaa ctgtcaataa cttatattgc tatagcccat
     5341 ttgctgagca acagtttgtg cttttgttgc gtatgctgcg cttatttttt cattccggat
     5401 ctgctctatt tgactgagcg ccgtcaaata ttgatcgtaa gtagcggtgg cagtcacttg
     5461 accatccttc aacatcgtat cgaataactg atctagttgc gtgacttgcg cgatctgagt
     5521 atcagcgaat cctagatatt gcataacaac cgttttccaa ggactatctt taaaaaagct
     5581 catattttca cgtacacgag cgacatctgc acttgtcacc ttttctttga tgacgacatc
     5641 gtctacagct tgctgccagt ttgatacatt tttagtaaat aaatcgttga cagctgtctg
     5701 gatcttcagt tttgattctg cactatctaa ctgtgtcacg attgctttct tctcatcttg
     5761 gattgcaaca gcttctttag gcaattctga ttctttgatg tcgtaatcat ctgctgaaag
     5821 tttgacagag gatagcttat ttgacaaact tgtgattgtt tcttctgtta tttcagggtt
     5881 tagaaaattt ttttcttttg tactatacag cttgtctact tcttttgata aagaagtcaa
     5941 ggtgtctttt tgattggtga tggcagattc tgcataattg atccgttgct gatgttgccg
     6001 ctgattattg atatggtaac caaacaaaca gatcgcaata gcacctgcac ctaccgccac
     6061 agcagtcaat gacttgacga atggcttttc catagattca ctcctaagta ttgatgatag
     6121 ataaattaag atataattca catacataaa aatgacagct ttttatttta aaaaacataa
     6181 ttaaattaat atattcacag aaatatttgt aaacaagttt tccctatata atcataccac
     6241 aaactatttt cttgactagg gcaatttacg atttaaaagt
//
LOCUS       DAYYKN010000143         6251 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000143 DAYYKN010000000
VERSION     DAYYKN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.145"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACHBB7_002499"
     CDS             complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACHBB7_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM9210395.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="ACHBB7_002500"
     CDS             499..861
                     /locus_tag="ACHBB7_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9210396.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="ACHBB7_002501"
     CDS             913..2595
                     /locus_tag="ACHBB7_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM9210397.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="ACHBB7_002502"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="ACHBB7_002502"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM9210398.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="ACHBB7_002503"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="ACHBB7_002503"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM9210399.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="ACHBB7_002504"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="ACHBB7_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9210400.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2076 a         1089 c         1426 g         1660 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt t
//
LOCUS       DAYYKN010000144         6174 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000144 DAYYKN010000000
VERSION     DAYYKN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.146"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHBB7_002505"
     CDS             725..811
                     /locus_tag="ACHBB7_002505"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9210401.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1622)
                     /locus_tag="ACHBB7_002506"
                     /pseudo
     CDS             complement(932..1622)
                     /locus_tag="ACHBB7_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1867..2781)
                     /locus_tag="ACHBB7_002507"
     CDS             complement(1867..2781)
                     /locus_tag="ACHBB7_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9210402.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2763..3143)
                     /locus_tag="ACHBB7_002508"
     CDS             complement(2763..3143)
                     /locus_tag="ACHBB7_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM9210403.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3593..3880)
                     /locus_tag="ACHBB7_002509"
     CDS             complement(3593..3880)
                     /locus_tag="ACHBB7_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9210404.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3900..4124)
                     /locus_tag="ACHBB7_002510"
     CDS             complement(3900..4124)
                     /locus_tag="ACHBB7_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM9210405.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4579..4749)
                     /locus_tag="ACHBB7_002511"
     CDS             complement(4579..4749)
                     /locus_tag="ACHBB7_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210406.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4764..5297)
                     /locus_tag="ACHBB7_002512"
     CDS             complement(4764..5297)
                     /locus_tag="ACHBB7_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9210407.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5318..>6174)
                     /locus_tag="ACHBB7_002513"
     CDS             complement(5318..>6174)
                     /locus_tag="ACHBB7_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM9210408.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1634 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa atataacccc tggcaataaa
     1501 aacctcttaa atcgaatttt agagcctttt aatgaatatc ttgtttgata agccttatag
     1561 ctaagccaaa taattcctaa aatactggca aaaatgagta gatagggtaa atagaatgcc
     1621 atttctattt attctcccca taccagccaa aaggctaaca gtgtcatgat taagcaacat
     1681 aagggggcaa gaatatccat tgatcgctgc ataattactt ttttctgttc tttatttcgc
     1741 tggtttaata gcgcatggtg gaattttttg acttgtgaat caattgtagt ttgtattctt
     1801 tctttggtat aaggtgcaag aaaagcacgt tgtttcttta tttcattttc tatgtcttgt
     1861 aacgttttag aacgataatt cttgttggta gtctgctcct ctttcaacgt gttggtcatg
     1921 gtttcttcga gtcggtctaa tttccggttg ttcagtcgta agagttcgtt gttctcttcg
     1981 tacttctttt gcagtttcgc gaatgctagc tcgagctctt cgtagctcgt tggttcgttg
     2041 ttgattgtag gctcgttctc gtctgtccag ctcatgtaaa atcgtctcct tttcgtaatc
     2101 ctttcctagt ttgttggatc gtactttttt ctttttgtgg atcaattcgt aagtaaaatt
     2161 tttcccacga ttcaagcaat gaatgccgaa tgttttaaga gaatcacaaa attggttata
     2221 cgttcgagtt tttttgtttt tcattgtttg atcaatcgca aaccgaattt cgtctttcca
     2281 aacaggttca tttttttctt ttaatttgat ttcagacatg gttcgttttt cgggtttctt
     2341 ccctcgttca gtgattgtta agtcatgttc aagacaaatc tcgtcattta ggtcataaca
     2401 tttgtttaaa aatgcttgac catgggcttg aaatttgttc ccagtttcca gattgacgga
     2461 gttaatgaca atgtggttat gaatatggtc tttgtctgta tgtgtgtaaa tcgcgatttg
     2521 gtggtcaggg gctatttttt tcgcatactc tgcccctagt tgattggctt tttcagcggt
     2581 aatttctcct ggtcgaaaag actggatcaa taagtgtgct tgtacacgat cgtcttttcc
     2641 atacagcatg cgaatttgtt tcatttcgct tttggcataa tcaatgtcac agtttatccc
     2701 tgttttgacg gttgctcgtg gttcggcgta attgatcgct cggctacaag aagtggttcg
     2761 gcttacttta actgttgcca tatcttttgt agctcctttt gcatgatttt taggttttcg
     2821 gataagcgtt gggcgtcgcc aggatcaacg tcgtgttggt gtgtgttgca ccacttggca
     2881 atttgattga tattcccgcc agctttggct aaatcgcgcg cgattgcctg agcaatgtct
     2941 ggcgccactt tcggtgtcac cagacgcgcg ttttgtgcct tggctttcac gaaagccggc
     3001 acactcattt gcaaagcttc agctgactgc ttcagctttg caaattcgga ttcgctcact
     3061 cgaaaactga tttgttttgg ctcggatcga taggtatatt ttttagaatg attgctagcc
     3121 aaatattgat tttgttcgct cataagtccc accctttcag tacgtcctac ggacgtatcg
     3181 ttcgcaatac cattgtacct aaaaaggtac aataagtaaa gctcactaaa gctcactaaa
     3241 gggcttggca agctctatat ttgctagcca aatacctacg tatttggtgc aaataaccgc
     3301 ttgtcaaagg ggcgcaagct cccccttgaa tccccctccc ccccactctc cacaatacaa
     3361 aaaagactag caaatgctag ccactttttg taaagtttcg cttgtttcac cttcggtgaa
     3421 attgttgcaa atcaataatt aggtagagct acattttttt cgttgttcaa agattcttat
     3481 gccatttact attaataaat ctataaatat gataattttt cggctctata atttatggga
     3541 ctgatgaatc agaattgatt cttcagtccc attttcattt tattccaaag aattaactcc
     3601 aaataccaat agaagcccat cctctatata cttgagattc cttactaaca atttcttgta
     3661 attctattaa agatttaggt aatccaaatt tatgttttaa aatatatctt ccaagaaaac
     3721 tacttaattt catactaact tcaccaacac tttttccctc actgatttct ttagaataaa
     3781 gaaagagctt ttcttttaag tcaggatagt catctatagt tgcttcattg tatactttac
     3841 taattgcatt taaaagtttt tcttctttag taaaatccat tataataacc tccctaccac
     3901 taccttctag gtgcccatgg accatgatta acccaaccat taacaataat ttttccaatt
     3961 tctcctttag cttgattcca atctacccaa catttttctt tgttacaata aagaccattt
     4021 ccatagtaag tagctgcatc aacttttatt cctgtaccga tgccagctag acaagttcct
     4081 aatattccta gaataacaca atgttttaat actttctttt tcatgattgt ttttcctttt
     4141 cgttttttta aaattgcttg ttatattaaa taatcttagt aattttatta tataacattt
     4201 tattatataa caatgattaa cgtcataaaa agtagttttg aattcataat cgtttaaata
     4261 aaattataag atcagttatt taaacagcta gtagacataa tgtgtatact accaagtaac
     4321 gcctgtacca agggatccag gcgctgattt tgtgaatttt tattggtcta ggttaaaagc
     4381 tctttttaag gcttggtttt ctgcggttgt ttctttctaa ttctttgtga atattgatcg
     4441 gtcatttttt aaatacttgt tatttttgtt tgtgtttgtt atgcttttta catatctcat
     4501 attcatttat tattcttttc taaggttaat aataggataa tctctttcag aaagactgct
     4561 cgtacgagca gtcttttctt atttatcaag tttttctaca aatacttcct cattttgact
     4621 gttgtagtaa ttaggattga gttttagttc ccctttttta tttcctttgt tggccactga
     4681 taataacatt gggctaaagc ttaatattcc tagtaggata gaaccgaata gcaagtgttt
     4741 ttttgtcata agtatttcct tcttcatttc catagctgcc accatttttt ccaattcttt
     4801 gtttgttcgt ccattgtttc tgttgcttca gaaagagtcg ttgaactatt ctctgaagac
     4861 tcttccgtat aagtcaactg cagttgctcc tgaagttttt cgatttgttg gttggcttgc
     4921 agggtgagtt gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctttg
     4981 agggcaagtt gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt
     5041 gcaaccacgt ttttgtttgg tatataagca tttttatcgt tgttttgttc atgtttatgc
     5101 aacttatcta gcaacctttg attgtctaag ttgcgagcaa gttgcaatac tccagaacgc
     5161 aatacaacct tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata
     5221 tgtttcgata ttgcttgcct actcatctta aaaagatctg acaattcctt tattgtaaag
     5281 gtttcttgtg gttgcatact ggttgcctcc taggttgcta ctggaataaa ttatctaaat
     5341 cagataataa actttttttc tctttttccg aaagtgcgga ctctgtttgt ttttcaagag
     5401 cttttttctc tttttctagt tgtttgattt gttggaggag ttcaagttct cgtttcttct
     5461 gtttgatttt ttcgttaatt gttgtagcgg gttcttcgta ttctttttgt ttatcagcta
     5521 aagccaatgc tttattttgg tcttctttgg ttggtgcatg tttgttaaag tcgtctgctt
     5581 ttttgatctc aggtttgaac gtaaattgat agccaataac aggttttccc cgtccttttc
     5641 cgtatttctt tttgattgtt agtcctttga agattgcagt tagttcttga cgtataggtt
     5701 ttataactcg tttatctata tcaggttgct tataactttt aggaatatct aataattcaa
     5761 gaaagtcttc tttggagaaa tatgcgaaac ctttggttct gtattgcttt aacagacgaa
     5821 acatagtctt tgcatagcca ctttgtagct cgttgaattg ttgtaaagaa tagcgtaccc
     5881 actcgtctaa attatttaac aaaggcagtg ctttttcgta aattcgtata tctacgtaag
     5941 gattttctac gtccccgttg attttaaatt ctgtaaacat tacaaacata tcgtaagaaa
     6001 gcccagactc acttctacga ccaaagcgta aagataagat tttttcataa gttgctttta
     6061 aatcttctac aaatcgtaca tttgcagttg ctttgtagtt acttaaatct tttagttggt
     6121 caaacgtaaa acgaactgtc ttatcccctt tttcacgcat tctagacacg atgg
//
LOCUS       DAYYKN010000145         6154 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000145 DAYYKN010000000
VERSION     DAYYKN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.147"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..1099)
                     /locus_tag="ACHBB7_002514"
     CDS             complement(137..1099)
                     /locus_tag="ACHBB7_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9210409.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1101..2540)
                     /locus_tag="ACHBB7_002515"
     CDS             complement(1101..2540)
                     /locus_tag="ACHBB7_002515"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM9210410.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2725..4671
                     /locus_tag="ACHBB7_002516"
     CDS             2725..4671
                     /locus_tag="ACHBB7_002516"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM9210411.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4935..5807
                     /gene="scrK"
                     /locus_tag="ACHBB7_002517"
     CDS             4935..5807
                     /gene="scrK"
                     /locus_tag="ACHBB7_002517"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9210412.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1958 a         1051 c         1093 g         2052 t
ORIGIN      
        1 atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac aactcctttt
       61 tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt atagacatta
      121 aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt aaaatataat
      181 ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa
      241 tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca atgaaagagg
      301 taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa
      361 ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat
      421 ttataatagt tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt
      481 tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag
      541 aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag
      601 tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag
      661 ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa
      721 gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt
      781 ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa
      841 ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa
      901 aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt
      961 tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc
     1021 ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat
     1081 cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct
     1141 gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt
     1201 tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt
     1261 tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct
     1321 gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca
     1381 tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt
     1441 tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt
     1501 aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct
     1561 tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt
     1621 agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca
     1681 tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag ttttactttt
     1741 tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc
     1801 tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact
     1861 agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact
     1921 tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt
     1981 tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa catttgtgga
     2041 tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta
     2101 atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca
     2161 tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg
     2221 gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga
     2281 aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca
     2341 aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga
     2401 tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt
     2461 acattatcct taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt
     2521 atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt
     2581 aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat atgacaaacg
     2641 tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt
     2701 aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa
     2761 gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta
     2821 ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc
     2881 acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt
     2941 tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa
     3001 attgctagta aagataaaaa attcaacccc attatggcat taattaaatt attatcagat
     3061 atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac
     3121 tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt
     3181 gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt
     3241 ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt
     3301 ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt
     3361 tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg
     3421 atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca
     3481 ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt tattggccct
     3541 gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata taacactact
     3601 ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta
     3661 catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga
     3721 catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca
     3781 tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt
     3841 gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc
     3901 tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt
     3961 aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca
     4021 acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt
     4081 acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa
     4141 caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa
     4201 tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt
     4261 agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc
     4321 attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt
     4381 gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt
     4441 ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa
     4501 aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt
     4561 cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata
     4621 gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata aatatatttc
     4681 cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat caaatatttg
     4741 agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta ttagaatttt
     4801 aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca tgttactaaa
     4861 taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat tctaaaataa
     4921 agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg
     4981 ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca acgacacctg
     5041 aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat ttagcaggga
     5101 ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact tatgggtaca
     5161 ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc atgaaaaaag
     5221 aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat ggtgagtatg
     5281 tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt acgggtgttg
     5341 gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca gaaatggggc
     5401 atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca tttcatggca
     5461 actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg aaaaaaggac
     5521 aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt gctcaatgtg
     5581 cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt ggtgtgatga
     5641 aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc aatgggtatg
     5701 tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac aacgctggaa
     5761 cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg aaaaaaggct
     5821 cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat aaccagttag
     5881 gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat gagataaatt
     5941 cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt gcaatagttt
     6001 tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat aggttctgtt
     6061 gcaaagtttt aaatctacta tcagataagg tagaataata gaaaaagata gcaggaggaa
     6121 tgacgatgag tcattttaaa ggaaagcaat ttca
//
LOCUS       DAYYKN010000146         6149 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000146 DAYYKN010000000
VERSION     DAYYKN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.148"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..937
                     /locus_tag="ACHBB7_002518"
     CDS             92..937
                     /locus_tag="ACHBB7_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9210413.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1218..2438
                     /locus_tag="ACHBB7_002519"
     CDS             1218..2438
                     /locus_tag="ACHBB7_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM9210414.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2435..5155
                     /locus_tag="ACHBB7_002520"
     CDS             2435..5155
                     /locus_tag="ACHBB7_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9210415.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5139..6083
                     /locus_tag="ACHBB7_002521"
     CDS             5139..6083
                     /locus_tag="ACHBB7_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9210416.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2296 a          963 c         1199 g         1691 t
ORIGIN      
        1 tacttactta aattaggtat aattagtata tgttttgtga gaaatttaga cttacaataa
       61 gcagtgaatt tataatcaac gtataggaga catggatgaa atgaaactaa ttggtataga
      121 tttagacggt actcttttga attctgacca gagcattagt caaaaaaatg caagaacaat
      181 gaaacattta ccggaagact gttttccatt catttgttct ggaagagaag tagaagacat
      241 taataatatt ctcaaaaaat ctggtttaag gataccagca gtagggttaa atggttctgt
      301 tggatttgat ggagaaaaaa agatatttga gtcttccttc gataaaaatg atgtaaaaaa
      361 aattgattct gtcctatcga aatttccaac caaaatatat acaaatctcg gaagttatga
      421 atctcagaat tataaaaata aaatgaaaaa aatttttcac gaggtaggga gtgaattttc
      481 tatcaaagag ctaaactatg agcttgagta tgaaaaatct atttgttcaa tcccttataa
      541 taatattgag gaaattatag aaaaggatgc tatcagcatt tacaaatttt ttatttttat
      601 tcctaatcgt caattgaaag ctaatatatt taaccatttg aaaaagatgc taaatgtgtc
      661 tgttactgaa tcggcacctg taaatattga aatcgtacct caaaatgtat ctaaaggtgt
      721 ggtgtttgat cgtttggaat caatctacaa tttaaagaaa cctttgagaa tcgcgatcgg
      781 agatagttta aatgacttat ctatgtttga aagtgcagat attagttttg caatgggaaa
      841 tagtcatcaa gaaatcatga aaaaagctga ttatgtcact gtttcaaatg ataaagacgg
      901 agtatctcaa gcatttgaaa gaattcttac catataaact aattaaggta gtatagaaca
      961 tagaattcta aatacggaaa aatggagcat aaattccagt acaaatagta cttcttcagt
     1021 gtcaaacgaa gcgaatataa catagatatt taggattgtg acaaaaatca tgtcacaatc
     1081 tttttttcga ataaacttat tttttgaagt cggccattta tcgtaatttc tttgcataat
     1141 acgaacaaat tttcggttat tgatgaaaaa aaacattgaa aatggtatga taaaagaaaa
     1201 aaaggaggaa atttttaatg gtgaaattta tccatgcggc agatttacat atggatcggt
     1261 catttgaagg actgacaact cttgataaaa ctgtacagga aaaactattg aagacgaatc
     1321 tgaccgtttt atcaaatatc attgaacagg caataataaa taacgttgat tttgttttac
     1381 ttgcaggaga taattttcat caaaatcgtc cttcattaaa aatacagaaa catttttcag
     1441 aacagatgaa acggttagaa aagaaccata tacctgtatt tataatattc ggaaatcatg
     1501 atttctatca aaaagagcgg tattggttct cctttcctaa aaatgtccat ttatttacat
     1561 ccgaaacggt agagacaaaa aaaataacga taaaaagtgg agaaactgtc tctctcagtg
     1621 gatttagtta tcgtcaaccg tggattcaaa aagacaaagt gatggaattt ccttctagag
     1681 aactcacaga ttaccatatt gggttgtacc acggggaacc tggcgtctct caaaaaggaa
     1741 actatgctcc tttccaaccc tcaaagatgc aagaaaaggg atacgattat tgggctttag
     1801 ggcatatcca tgtgccaacg gtcttaaatg aaaagcaaac aattgtttat ccaggtgcgc
     1861 ctcaaggtca tactaaaaag gaacaagctt ctacatctat tctactagtt gaattaagta
     1921 aaggaagttg ccaaatacat ccgattaaag tagcagaggt ctattggaag acaaaggaaa
     1981 tatcattgag gaaagcaaga acgacaaaag aggtcattgt tcacattcgt cagcagcttt
     2041 caaaagtaga ggatggattt agtttaattg agataaaatt aaaagattat gaccatctca
     2101 atacagatgt tttggaacgg atacaatcag gagagctatt agactatttg ttagaggaat
     2161 tctcagatag gataaatgat ttctttattt ggcgtatttc tctcatagag aatacttttt
     2221 atacgaagac cccacttgct gcttctgcta aattgatgga acagttattt caatactatc
     2281 agacacctca agatttccaa caaattttaa atgaagtata tagtcatcaa gaagcggcta
     2341 gaatattgag cgaactgccg gaatatcaag aagaaacact tgaaaaagcc aagcagctgc
     2401 ttaatcaaga ttttttattt gaggaggatc aggaatgaag attttaagga tcgagattgc
     2461 tgcttttgga aaatggcgtc aaaaaagttt tgacttttat tcaggcaatc agctgatcta
     2521 tgggggaaac gaagcaggaa aatcaacgat ttatcaattt attcaagcaa tattatttgg
     2581 tttcccttca aaaggccgta aaaaaaagga ttatacacca aaagacggct cagcctatgg
     2641 cggcaaaata tggcttaaac atcctgtata tggtgaattt gctgtcgagc gctacaagca
     2701 gcaaaatcgc ggaaaatcga aggtgtggct gggggatcaa gtgggaagtg atgagttact
     2761 agaaaaactg atcgcacctt tgacgaaaga gttattccag caggtattta ctttccagca
     2821 agagcagttg atggaactag atcatttgag agaaaaagag ctacatgatg cgctgatttc
     2881 tttaggcatc acgggcagtt ctcaagtatt ccaaaagcgg caagaatatt atcagaaagc
     2941 acaacaatta ttcaagaaaa aaggccaaaa attactaatc aatcaaaaat taaatgaatg
     3001 gaaagagctg caagaaaaaa ttcatcaaaa acaagaacag gaagcttatt ttggcaagtt
     3061 gatccaacaa gaacaggaat acgagtcgca acagcagcag ttattggcac aattaaaaga
     3121 ggcgaacaaa caattttcat tagccagaca gcaagaactt aatttttctt tatatgaaga
     3181 atggcaatct ctcaaaaaaa acagcactac aggtcttcct gatgagcaac ttcttttaga
     3241 tctggagtct ttctccaaac ggtatcagca aacaaatgag cgtttggaag aattagaagc
     3301 aacactggaa aagcatagcg ggatggatca gcagtcagcc agatattatt tttatttaga
     3361 gcaggagcag gaactaaaag atctccagca acttcaattt acagcggaac aattaaacga
     3421 agaatggcaa agattacaaa ttgagactag agaaacaaat tcagaattga agattttgga
     3481 acaaaggtgg cattggcaga aaaatccgtt accggaagaa tttttatatg aagaagaatg
     3541 gaagcatctt ctggctgaag aaagaaggat tgctgatcta gaaagccaga tttctgtgcg
     3601 tctccaaata gctaaagaac agcaagagat gattgaagca gaagtgaatg acttggaaga
     3661 gacctatcca gcattattac aaaatcagtc agagatagca ccagtctctt catctaaacc
     3721 gtatatgctg atggctggcg gagcaataat cattgcaggt atatttgtac caatgccctt
     3781 gaaagtgatc cttttactaa ttggtgcagg catgattggc tatttcttct acaaaaggca
     3841 accaaaaatt aaaactaaaa atgatttctc tgagataaaa ggaatgtggc aagaaaaact
     3901 aggacaactt gatctagcct tagctcaatt agctgaagca gaagaaaaac aaaagagaac
     3961 tggagaagaa aaacagattc ttcagcaaga agtatatcgt tttgcggatc atcggcactt
     4021 aggaaagatg gatacgctca aactcatgaa agaatatgga caggaagtca tagattatca
     4081 gaaattatat ggcgtatttg agcggaaaca taaaagagaa caggaaatac aagaaaaact
     4141 acgacaaatc gaccaaaaat ttgagtttgt taaagaatgg ttaccgatac aagataagac
     4201 aatttcagaa aaaatggtat tgctttcccg ttttatacaa gagatggaac agatgaaatt
     4261 tgccagaagc tatcagcaaa atactttatt gaagcaagaa atcaatcaat tgaaaaagaa
     4321 acaaaaagta tggatacaag aatacagcga acaattgact caagcaggct tatcttatcc
     4381 ttcggaagta cctgtctttt tacaaaaagc caaacaagcc gaacagcaga tgaaaagata
     4441 caatgaatta tcggaaatgc ttactccact atttcctgaa gagctgacaa aagagaaatt
     4501 atctgtaaga ctgcgccaac tgaaagaaaa gcaatcccaa ctccagcaag aagaaaaaga
     4561 agcaagtgaa aatcggcaac gactccaact tcaagtagaa gagttgcaaa gagacggcac
     4621 attggacgaa ctttatcaac aagctagccg gcaaaaagca aaattacagg aattactgga
     4681 ggaatgggga gcacttgaaa tcgccgggac attcttaggt gatctttcaa cagagatgag
     4741 tgaaagacaa ttaccccagt tgttaagagc agcaggtgat tatttaggta ttttaacaaa
     4801 aggaaattat cagaagatat tactgaaaaa tgatacactg actttaacag acggatatac
     4861 agaatttccc atctatgagt tatctacagg tacgaaagat cagctgatta tggcgatgag
     4921 atttgcttac ctctcattag aagctaaccg ttcgatatgc ccgatcatca ttgatgatgg
     4981 ctggcttcat tatgatcatc agcgaaagta tcaattagct gaacttttag ctaaatttgg
     5041 agaaaagtat caaataattt gtttttcttc tgataaagaa atggtaagct attaccatga
     5101 gatgaaccaa atcgtaaaac aattagaagg agcaaataat gaaaaaaatt cgtgatttag
     5161 taatcgatga gcagtttgaa caatttgtcc tgatcaaaag tgcagatgta agaatcgcta
     5221 aaaacggaaa aaaatttatt gcttttactt tccaagatac ttcaggtact atcgatggta
     5281 aatactggga tgcttcggag gaggaaatca ataaatttac agccggcaca gtcgtttttc
     5341 taaacggtaa aagagaggtt tatcaaggaa accctcaagt aaagattctt catatgcgct
     5401 taacaagggt agatgagcca ggagagccta gtttatacat ggaaagagcc cctttgaaga
     5461 aagaagaaat ggaagaagaa atcaatcaat tgttgtttga gatcaccaat gcgcattgga
     5521 atcgtattgt tcgctatcta cttaataaat atcaaaaaca attttttgag tttccggcag
     5581 caaaaagaaa ccatcatgca tttgccagtg gattagctta tcatactttg acaatgcttc
     5641 acttagggaa atcaatcgcc gatgaatatc ctgagctgaa tcgagcattg ttgtatgctg
     5701 gtatcatttt gcatgattta ggaaaggtga ttgaactttc cggtcctatg tcgacagaat
     5761 atacgcttgc aggaaatttg atcggtcatc tggtattgat tgacgaagaa ataactaaag
     5821 cctgtcttgc gttgaaaatc agcgaagctg acgaagatgt tctcgtatta cgtcatatgg
     5881 ttttagcaca ccatggctta ttagaatatg gctcacctgt acgtccaaga ataatggaag
     5941 cagaaatatt acatcaaatc gacaatatcg atgcttcgat gcagatgatg ttgacttcga
     6001 ttcgtcaaac agaaccagga aaatacaccg atcggatttt cggtatggac aaccgaagtt
     6061 tctatgtacc aaaagattta tagcaaaaat ttattttttt cattcaaaac aaaaaaagcg
     6121 agacataagt cttcgctttt tttgttttc
//
LOCUS       DAYYKN010000147         6079 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000147 DAYYKN010000000
VERSION     DAYYKN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.149"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(699..1202)
                     /locus_tag="ACHBB7_002522"
     CDS             complement(699..1202)
                     /locus_tag="ACHBB7_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9210417.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1561..2586
                     /locus_tag="ACHBB7_002523"
     CDS             1561..2586
                     /locus_tag="ACHBB7_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9210418.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2909..3787
                     /locus_tag="ACHBB7_002524"
     CDS             2909..3787
                     /locus_tag="ACHBB7_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFM9210419.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4263..4379
                     /locus_tag="ACHBB7_002525"
     CDS             4263..4379
                     /locus_tag="ACHBB7_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM9210420.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5307..5645
                     /locus_tag="ACHBB7_002526"
     CDS             5307..5645
                     /locus_tag="ACHBB7_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210421.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5739..5969
                     /locus_tag="ACHBB7_002527"
     CDS             5739..5969
                     /locus_tag="ACHBB7_002527"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM9210422.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         2082 a          903 c         1114 g         1980 t
ORIGIN      
        1 tcgaaaaata gatagttagt ttttccacag tggataactt cgtaagagat aaaaataaaa
       61 aattagtcaa agaagggcgt atagatttct tttgatagga gatatacaac agaaatgaat
      121 aaatattaac gtgttttcaa acagtataaa acggtgtggt ttttggaata aaaaaataac
      181 atatctcatt ttacactttt gttattttat acttaaaaaa taacgacaca gaaaataccg
      241 tgtctcagat ttctctacta cttctaaaaa ctactgtttt tttcctttga aatgccttca
      301 aaaagtagga tcaggcagaa tatactggca gaatcaagga gaagggagcc taaataagga
      361 gaaaaaccat agaaaatata aagtaattac ttataaaaca agctttttat agaaaaataa
      421 tatacctgag tagggtataa attaagacgg aattcctttc tagattgaga tattgactag
      481 gaagaatgtt aagattttaa gcctttaagc taaaaaaaaa ccctttttgt taaataaggg
      541 gaatatagcc tgtcagtttc aaactgcaag tcgtcctgct gcaataatat agtagattag
      601 agatgaaaat agattttttt aaaccaatga ctgaataaaa agaccgtcat agatacacat
      661 tggaatgtgt ctattacggt ctttttttac gaacgatttt attgaagacg gacagttcgg
      721 gtcaaaaata aaaaagctac gatcagaaat aagaataaaa tgtatcccaa aataatggct
      781 agacggtcta acttatttgt cgatagatgt ttgtttagaa gttgataggc ccataaacct
      841 atcagtcctc ctgttgtatt agttatcaaa tcagtgatgt ccgctacgcc aataccgaat
      901 aggaattgaa tcaattctgc caagaagctt acaaggaaaa taatccctaa ttttttagaa
      961 aaagaaagtc tcttgaaatt gacattcaaa agcaccccaa aaggaaaaaa gacaatcaag
     1021 ttataagtgg tctcacgtaa taccgtcgta ttttcaaaag aaaaagtaga aaaagggacg
     1081 agattgacat tgctgtaaga ataagtgaga aattgtggaa gattaaatga tagcttgaat
     1141 aaaatcaacc ataatagaaa gaataaataa atccctaata aaaattttga tacataccgc
     1201 atagacatct cctccattta aaaagtatac catcgaaaac cgctgaaaaa tcataaaaaa
     1261 gtatgaagtt taaggaccaa cgtagttatc gtacgaaaat tagaagcttt tttttgttgg
     1321 gaatccggtt tctttaacaa agctaagata gcgagttgct tggaaaagtt gctatctttt
     1381 ttctgtcaaa acgactcgga atgttcgttt tttgttctaa tttctccaag ttgtttactt
     1441 tttgatacta agtttatgaa aaatcaagaa atagttgtta tactaggtaa tgctgacaaa
     1501 cgggattggc atttcatctt ggttcagctt tttttccaat tactaggaag gagggataga
     1561 ttgttcaaga cttattttcg tttcaataaa atacaagata atccttttcg tatcatgaat
     1621 ctgatgctac ttatctttat tgtattatgg ttaggttaca taaatcatga ttttttgatt
     1681 tcaagttttt catcattagg gatctttact tttttctatt atcaaaacat accgatgaaa
     1741 tatttgctga aacgtatgtt tctgatcggt tgcggactgt tggttgcttt cgttttgggg
     1801 atcttatcga cttatgtttt atggcttgaa ccatttgccg tagctttggt tgctttttcc
     1861 agcagattcg tgttgcgatt attccatatt tctaaaccag gtggcttgtt ttttgcgatg
     1921 ctttctgcca tgggaacgag catgcagctg ccaatcgctc aattaccgat cgtctcactt
     1981 tattttttta tgggtgtcgt ttttgccctg attgcggcag tgattacaaa attactggat
     2041 tcacggcctg agcagacaat tgaaaaagca actttaaaag aaagatttca cgaagagcct
     2101 ttagtgatca ttgattctgt tttctacagc gctgccttat ttctttccgt ttatgtcagt
     2161 cacggcttga atctgcataa tccttattgg ttgaccttgt catgtgcatc gattttgcta
     2221 gcagaaaact tggatgcgat gaagcatcga caagtccaat atctgattgg atcgatgggt
     2281 gggttatgtg tgtcggcatt cttgtcattt gtgccgttta cgcagttgca gaccattttt
     2341 ctgatcactt ttctatatgg aatcgcacaa tttctagttg ctagaaatta tgcagttgct
     2401 aatatttttc ttaatccgat ggcgttgatg ctatctacac ttattcgtgg agcatacttg
     2461 atttctttga tcgaatatcg ttttttaggg atcgtcatag gaagtttcat cggtttaggt
     2521 gttgcgtggg taatgacggt tggtttgcaa cattatttaa cggttgttcg tcaaaaatta
     2581 gaatagaagg acaaatatat gtgccaaaag aggttatgac aagatttttg tcacaacctc
     2641 taattcaagg tgttcaaaag ccagattctc agcattaagt caaattcaca caaaaatgtg
     2701 gaaaggaact ttcaaaaaaa ttttatattt attctatcaa aaaattttgt ttatgatggt
     2761 aactgaataa actttcctga gatggtcaat gctttccttg aaagtcttac agatattctt
     2821 tcttcataga aagtttaagt tttatagtta gtgtacaata aaagtatgtt tataccatat
     2881 ggtgtccgga taatcagtca tttaaacaat gaggttcaaa gatatgaaaa ggggaaattt
     2941 gatgaagaaa ataagcaatg aaatccaaat tctggcagaa actttaactc ttcccaaagt
     3001 cgttatagag caaatgattg tacaagcaaa aaacatcctt gatgaggtgt ttgagcaata
     3061 tagtcatgaa ctaatggatc ctaataaagc aaaggcgact gcaaagaaaa tagacaaagt
     3121 ctatgaagag gaaccttttg cagcattagc tatttatcta tatgcagctt ctcaaagttg
     3181 gaaaaaaatt tatcaaccat tgaagattcc aagaactatc tatttggcaa caatgaatgc
     3241 ttttactaga tttattcatg aacatttcca agtaacagga agctatcgct ttgaccgtgg
     3301 tttttggatt tggcattaca ctagtggctt gatctttcgg attggtgaat tggagtacga
     3361 aatgacatat tttccgaaag ggtacacgga tctaagatta gaaggaaagc agtgcctttc
     3421 tcttcatatt ccttccgatg cagatttatc tcagaataag ataagagata gttacgaaag
     3481 tgccattcgt ttttttgaac tttattttcc tgattaccat taccaagtga tgtacacaga
     3541 tacttggtta ctatcaccca atcttactaa atggttgaaa aaagagtcaa aaatctgtct
     3601 ttttgcagcc gattatcgat tattatcagt tgatgaacag gacgatagtg gagtcccttg
     3661 gatctttgga cgagtagatg cccaaataca tgattatcca gaaagtacga gtttacagcg
     3721 acaagcaaaa gagcaattgc ttgcaggcga gcacattggc tctggcttag gtattcttac
     3781 ttcataagtt tagtacttcg aaatagttta aaagagcgtg agacaaaagt aaagaacact
     3841 tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata
     3901 agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag
     3961 ttaaacgctt ctgtcccagc ctctttttta gtgttctatc gaacacacag tacgttcaat
     4021 aggtaaacta gctataacaa tagatattaa agttaacgga caagagggcc ttttttaggt
     4081 agctaaaacg taataatgaa aatttatttc cagagatagg agtcaatcag ctagtctgat
     4141 cgactgtttt aacaagttga ctttcatttt aacttttgat accatttaaa acacaaatcg
     4201 taattgtttc gatttgtgtt ttttagcatg actacaatca aagaagaaag tgggtcgtcc
     4261 atatggaaaa aacagatatc tcaagtgcat accgccgctt gaaaagccca aatatcaaaa
     4321 cacgaaaacg tgcgctgaag atcatcaaag atgtgaaacg gaatagtggg aaaaggtaag
     4381 aagaaggaaa cttaattaaa aaaatagccg aaaaacgttt ttcggctatt ttcaataatc
     4441 ggtttttgtg taaggtttcc gaaagaactg ctgttgattt tccattttta acatatgata
     4501 aattggtaga atctttcttt tcaaattgat accattccct aattccagtt atatagttaa
     4561 ctacagaacc acaaagcacg tacgtttata aaagtattat ctgtagttat ttttcaattc
     4621 cctttcaacc tcttattttg accagtagta ttgatcactt taccttttaa cacctttgtt
     4681 cgtttttgaa acatttttag tcaagaatgg aaacgtaact acataatcaa aggttttgcg
     4741 aaatatttca tgaaatattt catgtagaag cgagcaatac ggttgcaaat aaccattatc
     4801 gtttaataag cagttacgaa agctcccaat atctctaacg ttcagctcac tccatttctt
     4861 ttcttcaaaa aaatcagtga ttcgtatctc tttctatttg tcgaaaaagt taactttaaa
     4921 aagtaacgcc gaaaaaatat gcaaaaaaat aaaaaagcag ttactattgc tagaataaca
     4981 tcaaggaata ataagttttg gttttcagat aatagtcgat aagttgtgta ttggaatttt
     5041 agatacagta ataaagtatt tttcatgaaa ttttgtgaga atcactatta caagtatctt
     5101 taataaaatt aatggttaag gaggaactta aagtagaatg taaatgaata gttgtagtca
     5161 gtcgattgtg atactagact caaagttgca ggaataagtt tttcaatttg tgattggaag
     5221 ataaaaaatg aaaaagaagg gctttttttt atactttgtt gattgtagtg tttattttcc
     5281 ctctgtttag ccaagtaaca ttttctatga ctcaaccagt ttcaagtgag gaacatgata
     5341 acctttttcg tgctacagaa gagtttaaga aatatcagga aaatattgtt ccggagactc
     5401 cattagctag tgagccaatt actgatattg atggaaacca aataggatgg tatatacagt
     5461 acgaagttat ggtggtaaat aatgactcaa tagtaaaaaa tacagtaggc ttagagctaa
     5521 caccgattga aaaggtgagt tcattgttaa cgtatattta tactgatgaa agtgacgaga
     5581 ctgagatcat tttaacggat tactcaaaaa ttgctactga taaaaagtat atattgtgga
     5641 tgtagacact agtgaaatac tttatgatat agaaattgat gaaaatttac tagatgtagc
     5701 acaagaaatc gatcatcaaa agcaggaagc tattcgatat gctggtagaa aagggaaaag
     5761 aaaatattta ttacgtgaat gtagcaaaag ttagagaaga tgaaaatgag tggaaagaat
     5821 tcaagagtcg ctactcaatt aactcgactc cgacatttac agtttacaga gaaggatcga
     5881 tagaaaaaac tgttttttgg actaaagaaa gtggaatgtc tttagctgaa gtagaagagt
     5941 ttttggatta tgttagtatg caacagtaag ggagttctta ttatgaaaaa ttttgacaca
     6001 tattatgtta ttgttttttc gatatctttg ctgttaatat tactaacttt aatgcagtat
     6061 aaagagtatc cagagtatt
//
LOCUS       DAYYKN010000148         6075 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000148 DAYYKN010000000
VERSION     DAYYKN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.150"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..455)
                     /locus_tag="ACHBB7_002528"
     CDS             complement(<1..455)
                     /locus_tag="ACHBB7_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210423.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGT"
     gene            complement(555..1064)
                     /locus_tag="ACHBB7_002529"
     CDS             complement(555..1064)
                     /locus_tag="ACHBB7_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFM9210424.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1265..2134)
                     /gene="scrK"
                     /locus_tag="ACHBB7_002530"
     CDS             complement(1265..2134)
                     /gene="scrK"
                     /locus_tag="ACHBB7_002530"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9210425.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2154..3275)
                     /locus_tag="ACHBB7_002531"
     CDS             complement(2154..3275)
                     /locus_tag="ACHBB7_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM9210426.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3277..3672)
                     /locus_tag="ACHBB7_002532"
     CDS             complement(3277..3672)
                     /locus_tag="ACHBB7_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9210427.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3691..4488)
                     /locus_tag="ACHBB7_002533"
     CDS             complement(3691..4488)
                     /locus_tag="ACHBB7_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9210428.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4490..5278)
                     /locus_tag="ACHBB7_002534"
     CDS             complement(4490..5278)
                     /locus_tag="ACHBB7_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9210429.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5292..5768)
                     /locus_tag="ACHBB7_002535"
     CDS             complement(5292..5768)
                     /locus_tag="ACHBB7_002535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9210430.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5840..>6075)
                     /locus_tag="ACHBB7_002536"
     CDS             complement(5840..>6075)
                     /locus_tag="ACHBB7_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9210431.1"
                     /translation="SFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQ
                     THFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         1879 a         1319 c          870 g         2007 t
ORIGIN      
        1 gtgccagaca agtgatttgt attgagttga taaaaaagat ctccatctaa aaaaatatca
       61 ttagaagaag atttccccac agtaatgacc attccatcag ctctatgtgt tacatacaaa
      121 taattgtatg acctcatttc acctaatgaa tcaattattt cttgcccttt agctctaata
      181 tattgatctc tattacctat ctcactctta tttaacaaaa gttttttcat gtcaaattta
      241 atcggctgtt ttatttttgt aaattcgctt ctactactaa tccaatccga gattaatttt
      301 tcaacatgtc ccagagtaaa ctctgattta ttcgctaaat actttttttg ttgtattaaa
      361 gacatctttc cccgaagctc aaaattgagc ctacatcgat tctctatctc ttcaaaaagc
      421 atcttatctt catcattctt atattcatca ttcataaaac caatccagct cctcgattaa
      481 gataatattt taatctcata caaaaactag ctctcatcaa aacaaccatt gttttgattt
      541 ctcatgaaat ttctttatac ttttctccga ttataaattc ctaacccctt gagaaaatta
      601 cgcatatatt tatctccgca aggcttaaaa tttcgttggc cttcttttct caaaaatgcc
      661 ccgatttcgc cttttgatgg atagatttct gcctcagcaa aaacgtctaa aatatcatca
      721 gtggtcatag acaaggcgat ttttaatttt tttagcaaca cattgttgat atatttgatt
      781 tccttgcttt tttgcggcgt gtctactatc ggcttatctt tttgcggacc acgcgccaag
      841 agaatcaggc catttaggaa tgcgtcaaaa acttggttgt tgatggtttt ttcatacaca
      901 ttattttctg cattatcttt gttcttatcc tgtactttgg tcagtaccgc ctgagcttct
      961 tctttagtca gtggtacacc gcctagctca aaagctttaa tcaaatccgt atctttcaaa
     1021 tcaatcgcat aacgcaatcg caataaccta tcattgttat tcatttgttc ctccgaagta
     1081 tttagtcttt actttcttac gtactttttg tcttgctaag atttttagga tcaatgataa
     1141 aaaccgcatc acttatgata aggcgctggg tatcatgagt gaatgatatt gctgaataca
     1201 aatagtccga ccacattaag ttgatcggac ttcataagca ttttattgac taatactttt
     1261 ttgcttaaga attgattcta acatcttttg ctaaagttaa gcatcctata gttcctgcat
     1321 tatctgctaa cttaggtgtg acaatgtatt cttcgatatt tggcgtatcg acatagccgt
     1381 taattagttc taaaaaacgt ctttgtactt tctttttcag atgttcttgt tgcatgacac
     1441 caccaccaaa aattataata tctggtgaaa gcatcagggt cgtattacag gcacattgtg
     1501 caatataatc cgcctcgatt tcccagaacg gatcttctat agaaagatct tgtcccttgg
     1561 ttcccgtacg catctcgata gcgggtccag atgccaaccc ttcgagacaa tcatgatgaa
     1621 aaggacaagt accagagaaa gtatctcctg aatgtctctt aactaacgta tgccccattt
     1681 ctggatggct aaacccttca acaaaaatcc catcttgtat agctcctcca ccaattccag
     1741 ttccaacagt gtaataaaca acactggata aaccttttcc atgacctgaa acatactccc
     1801 cgtaacaagc agcattcaca tcggttgtcc aggcaattgg aatttcgaaa tgcttattca
     1861 acgttccaac aaaatcaaaa tttttccagg cagtttttgg agtagaggtg atatgtccat
     1921 aggtctttga ttcacgatgg atatcaatcg gtccaaatga ccctactcca atgctttcta
     1981 attgttcttt atagtgatta aagaaatcaa ttaccaaact cattgtttca ttcggtgtag
     2041 ttgtaggaaa actcactctt tctaaaacct ttaaatcatc attgccaatg gcacaaacaa
     2101 atttagtccc acctgcttct attgagccat acatttctta ctccaccgtc cttttatcta
     2161 ggaattcgtt cttccctgtc taatagtttt tcgaaaaact catgtggttc cttttggtac
     2221 caataggcaa cagaagaaat atcgtcttga cgctcataaa gcttgatgtc atcatttcct
     2281 agggtttgga aagtaacacg taaattttct ttgaaactaa tggggtcggg taaatgccag
     2341 cggtacaacc catgcatcgg taaagcatca tcgccaaaac catgaactgg atttggatta
     2401 ggttttccag cactaaaata atcccgggtg tgatctcttt tagattgaaa aggatagccc
     2461 ataaatagcg tattaaaatt ttgagcatat gccctttctc caaagttctt tttatggaaa
     2521 gcccaagcgc cgccaaaata atcttctgct cctgtagatg ttattgtagg gaaatcttca
     2581 tctccatcta tataaaactt aaattctcct tccccccacc agtatctttc caatgcgcac
     2641 aaagcaaagt aggttcctac ataataccca gtgcctttga ttccatcaat aagggtgtaa
     2701 tcttttcctt ttgtcgtgat gggttcacgg ttccatttag catgaaaata catcgcatca
     2761 tccggtactt cagtcaatgc ataattaaat gtataaaaaa tgtgcttaat aaactcagga
     2821 tgttcattcg ttaaagtaat cttagctttt tttcgaaatg gcatctcaaa atagcaattc
     2881 atccctgatg ttggattcac tacgattggt aaggaattca cttcgcatct tgtcccaaac
     2941 ccattacaaa aaaaatctcc taatggactt tctacagaag gagtggattc atcatcccaa
     3001 taaatacgca aaattaaatc acgtaagacg aaactcccag cgattgtttt gtctgtcact
     3061 gtcatccaaa aatgacgtaa aattccaggg ccttccattt ctgctaaagt aattgtctct
     3121 ccagcattta tcggaacaga aggtgaccct tttctcccaa ttcctagatg actcgctgtc
     3181 attgccgctt tccctttttc accattttta ttttctggtg aaatagttct actttcctct
     3241 cccttgtgga gccatatatt atttaaacaa ttcattttat tcgtcctcta tcctttcttc
     3301 acttttttga ttattcatat agatgatttg ttgttgtgcc tccgcaataa ttgctccaat
     3361 atctaaatta tcatttccta actgcaaagc gattgacatt gccaagggaa gattcattcc
     3421 tgcaattacg tgcactctgt cagaaatata ggtaaaaaac ttttgattta cacttcctcc
     3481 agccatgtca gttaaaacaa ttatttcgtc tgtagtatcc actaactctt taatttgttc
     3541 atctatatca tcaatttctt cgacatatgc acataagcta ctaatttgat tatgcatacc
     3601 tgttaaaaac tctagtgtct ctttaaaacc ctcagctagt attgaatggc tagcaatgat
     3661 caatcttctc ataattcaaa tccttttcct ttacgctaat attccaaatg cagccataac
     3721 catcgcaaaa acaataacga gtagaatcaa aatggtcata ctgatttttt tcttactgag
     3781 tagataatat gctgcagcag taataattac tggaattaat gttggtaaaa ttttgtccaa
     3841 cattggttgg attttcatac taacttcacc aaaagtgaat ttaagtggcg ttgaaacact
     3901 gataactgaa gcaattaatg atccaactac tgttagcccc aatactgaag cagcttcagt
     3961 taaataatta atcttttcac cgtattcagt aacaatcttc attccctgct tataaccaaa
     4021 ttcaaagcct ctcattcgga aaaagaagat taccatgatt aaaatgaacc ataaaaagat
     4081 tcctacagga ttattttcta aagccatata agccgcaatt gatcccatga tggttgggat
     4141 catcgtcatc aaaagagagt ccccaatacc agcaattgga cccatcgtac caactttaaa
     4201 gtcattaatc gcatcttttg cttctaatcc ttgcttttcc tccattgcaa cacaagcacc
     4261 taaaataatg gctgccagcc aaggctgtgt attgtaatac tgaaagtgat tatttaaagc
     4321 ttcgatgtat tcatcatcat ctttgtataa ttttcttaaa ataggtgaca atgcgtaagt
     4381 cacagatgca ccttgctgtg tttgataatt aaaggtacaa actgacatca accaacgata
     4441 gttcgctttc ctaagatcct ttttcgttac ttgatatttt tcgctcattt tattcatcct
     4501 ccccaataaa cccaacatca gaagctcccg cagaagcagt gatttgtgca tctttttcag
     4561 ctctatgata cacattaaat gctaaagcga atcctatgat cgccgctcca ataataggta
     4621 ccttcaaaaa agcggctaag aaaaatccaa caagtaaata actaatgtat cgacctgtag
     4681 gcataaagcg taacaacatc gcaataccaa cagcaggcaa catttttccg gctaatgtta
     4741 gcccatccat aaaccaatta ggtaaaacat ccaaaacgta attaacagca gactgtccta
     4801 aagtaacggc aataaatact ggaattgctg cagataaacc aaagaaaatt ggccccaacc
     4861 acaaaacact attcattttc ttaaattctc gtttattcgc atattcctgt gatttgcgaa
     4921 caacaaaacc atttaagatt ttgacaatta catctagttg cacccctaac atacctacag
     4981 gtagtcctat tgctaatccg gcttctactc caagctggct accaaatgcc gttgcaataa
     5041 ttgcgcacgt tccatagtcc ggtacagatg agccaccaaa accggcaact cctaatgaca
     5101 tcaattgaac tgtaccgcca actatcaaac ctgtttttat atcacccatc aaaattcctg
     5161 tgaataatcc ccaaaaaact gcgtaattta caaagatcat cggaatccca taataatcaa
     5221 catgtttaat aaatgctaat atagtcagca taataatttg aaaaatagta aaatgcatta
     5281 tccttactcc tttatcctaa ttttgattga atttcctgtg ccttatccgc cggaacaaat
     5341 tgtgtaacaa tttctacacc agaatccaac aattttttat acgtgctgat attttcatct
     5401 gatgcatagg ctctagaaga tagctgtaat gcattatctt taacaacagt accgccaaca
     5461 attagtcggt caattctaat accgttatct actaaatgaa ggattgtttc cggctctttc
     5521 acgatgacaa atactttttc ctgatcgtat ttcccctttt taaaattcgc aatcgctgtt
     5581 tcttcagtga taattgatac agccattcca gcaggtctag ctaaacgcat acttgatttt
     5641 tggatgtcgt tatttgcgac agaatcatcg atcaccatca ctctgtttgc tttagacatt
     5701 ggtgcccatt gcgtgacaat aatcccatgc aataaacggt tatcaactct cgacataaca
     5761 atactcataa atacttcctc ctttatcttt cattttctta caagcaatta ccatgccaaa
     5821 acaaaaagaa cttgataaat caagctcttt ttacataatc ataaacgtat ttaacctcat
     5881 gaatcgggat gtcgacacta taataatgtt caacgacact gaaagattgc ttaaaatgtg
     5941 tctgaaattt ttgaaattcc tcactcgttt cattgaaata aatttcatct tccaaagtat
     6001 tttgttttac taatcgttca attaagcaac taatgtgaac atataatccg aaacacgtat
     6061 tattatcaaa tgaaa
//
LOCUS       DAYYKN010000149         6068 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000149 DAYYKN010000000
VERSION     DAYYKN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.151"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACHBB7_002537"
     CDS             832..1566
                     /locus_tag="ACHBB7_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9210432.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACHBB7_002538"
     CDS             1582..2751
                     /locus_tag="ACHBB7_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9210433.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACHBB7_002539"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACHBB7_002539"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM9210434.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACHBB7_002540"
     CDS             3841..4767
                     /locus_tag="ACHBB7_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM9210435.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACHBB7_002541"
     CDS             4786..6036
                     /locus_tag="ACHBB7_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9210436.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAYYKN010000150         6006 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000150 DAYYKN010000000
VERSION     DAYYKN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.152"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..907)
                     /locus_tag="ACHBB7_002542"
     CDS             complement(32..907)
                     /locus_tag="ACHBB7_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9210437.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1055..1387
                     /locus_tag="ACHBB7_002543"
     CDS             1055..1387
                     /locus_tag="ACHBB7_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM9210438.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1499..2707
                     /locus_tag="ACHBB7_002544"
     CDS             1499..2707
                     /locus_tag="ACHBB7_002544"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM9210439.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2861..3316
                     /locus_tag="ACHBB7_002545"
     CDS             2861..3316
                     /locus_tag="ACHBB7_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210440.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3678..3953
                     /locus_tag="ACHBB7_002546"
     CDS             3678..3953
                     /locus_tag="ACHBB7_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210441.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3966..>6006
                     /locus_tag="ACHBB7_002547"
     CDS             3966..>6006
                     /locus_tag="ACHBB7_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM9210442.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFA"
BASE COUNT         1937 a         1018 c         1269 g         1782 t
ORIGIN      
        1 ctttcctcat agcctatctt tgtctttttt cttatagttt tttcagtaat cttgtctgtg
       61 gcttcaaata agtaaagcct tgcccttcca cttcatgcac attgatgatt gaaaggaaag
      121 ctttttcgtc aatctcgtgg acgatcctct tagcttcagt taattcacta gagctaacga
      181 ccatatagat gatttttttt gaattaccag agaatgcgcc ttctccagaa aggaaagtca
      241 cacctctttg cagaccggtc atcagtagtg gtgcaatctc ttcgcttttg ttggagacga
      301 caagtagacc ttttgcagaa tatccgccat ctaagatcga atcaatcaca cgactgaaaa
      361 catacgaaac aatcaacgta tacatcatac gtttcaagtc gatataggtt aatgaagcaa
      421 gtaagacgat gatatcaaat gccaataggg aacgccccat agtgatgccg taattttgtt
      481 ctaaaatccg agcgataata tcactgccgc ctgttgttcc acccatgcgg tagacgatcc
      541 cgcttcctac acctgcaact aaacccgcaa gtagagaaac gatcagtaga tcatgctgta
      601 aattgatttc tagtggaacc ctttgccaga tccataaaaa gaatgacaaa gaaagtgtac
      661 ctagaacagt ataggccaag gaacgtttac cgaggatctt tcctcctagt aaaatcaatg
      721 ggatgttgat taataaagtc gtataagctg gatcaatacc aaataaagct cttaagatca
      781 aggtgatccc tgagactccc ccttctgcta aatgattagc gatgttcaag taaacaagac
      841 caaatgcata aatacttgtt cctataatga taaaaagaac ttcttttact gtttttcgtt
      901 tgaccatatg aattccagct ttcaattatg tattttcaca gaatttccat ttagaaattc
      961 cttcctaaat gtagcatgga aattcgattt tctcaattgc tcttatctag gatttgcgtt
     1021 aaagttttgg taagatagtt ttggagggaa aaagatggat aaaaagcgtt ctttatacag
     1081 tatgcagcag gaagtggatg aatatatcca gcaattcaag gtggggtatt tctctccatt
     1141 agctcagatg gctcgattaa ctgaagaagt aggtgaattg gcaagagaag tcaaccacac
     1201 ttatggagaa aaaagcaaaa aggcttctga accagttaac tcggtagcag aagaacttgg
     1261 tgatgtttta ttcgttacga tgatcatggc taattcattg aatatcgact taactgaagt
     1321 ttttgaaaaa aacatggaaa aattcaatca aagagaccat aatcgctttg agcgtaagga
     1381 cgagtaaaca agatgaagtg gtcctggcct tgaagactct gaaacggact tgtcaaacag
     1441 tcaccatgca actgagtctt tatctcagga caagaaacac gcaggaaagg ttggaaaaat
     1501 gaaattaaaa caattacctc tggaatacca aaaggcaatt ccagttctga aaaaattaga
     1561 gaatgcagga tatgaagctt attttgtagg tggaagtgtt cgtgatatct tgctgaacca
     1621 gccaatccat gatgtggata tcgcaacaag cgcatttcca gaagaaatca aacagctttt
     1681 tccaaaaacg atagatattg ggatcgaaca tggaactgtg ttggtattag aaaatgatga
     1741 acaatatgag atcaccacat ttcggacgga atcaacttat caggactacc gtagaccaga
     1801 tcatgtcgaa ttcgttcgtt cgttggcaga agatttgaaa agaagagatt ttacgatcaa
     1861 tgcgtttgct ttgaaagaag atggagaaat catcgatttg ttcgatggtc tgcaagatct
     1921 agaagaacag atcttgcgag ctgtaggcaa tcctcatgag cggttccatg aagatgcctt
     1981 acgaatgatg cgcgggttgc gttttgtcag tcaactaggg ttcaaattag aggaagaaac
     2041 cttctacgcc atcaaagaaa accacgagct attaggtaaa atatctgtag aacgtataaa
     2101 tgtggaattc atcaagatgc tcttgggaaa tgaacgggcc gcgggaatcc atgcatttat
     2161 tgaaacagag tgctacattt gctgtcccgg tttgaaagaa gaaggcgaag cacttttacg
     2221 ttttgctgaa ttaccatccg tcccattgaa agaagagagc caagcatggg cattattgat
     2281 cagccaactg gatctaaaag aaaatgagat tcgttccttt ttgaaatcgt ggaagtgttc
     2341 taaccaaatg attaaagacg ttcaagcact tgtccaagga ctacgacaac gagaaactgg
     2401 agctttggaa ccattcgaat tgtacttctt aggcaaaaaa catgcactgc aagtagaaga
     2461 attgatgatc tattttgacg aaacacctca gatggatgcc gtaggagaat cctatgatcg
     2521 tttacctatc aagtcattaa gcgaattagc agtagatggt aaaatcttgc tagaagaaac
     2581 tggaaaaaaa cctggtaagt gggttagtga agctttgcaa ttagctgaaa aagccgtcgt
     2641 agaacaaaga ataaaaaatg ataaaaaaca ggttttggat tttttatcga aagaaaaatt
     2701 gatttaaaaa acactagact gtatctgctc agctttgttg gctacttaaa gtgggcagat
     2761 acgtcattag aagaaaaaac ataagatgtg accacttaca taaatttgtg ctaaactgag
     2821 gtagtacaag aataaattac atcataaagg agtcttttta atgagtaaag aaatgacagc
     2881 gctaaaattt tattttagaa atggagaaac ctggacgatc aaccgccgtc atatcggcga
     2941 tctttggatc aaacaaatca caacaagttt tggacgaatc aatggcagtg aatttgttga
     3001 aatccatccg tgtgctggat ttaaaatcga aatcttccat gaaggggacg cagttgcgac
     3061 acatgatatc aacctaggcg gactggaaat gggcatgttt aatcgcgcat tgaaatacga
     3121 agatatcgag cgaatggaaa tcctttaccg aaatggcaca cctgatcttg tgtacttccc
     3181 atatctagat aaaggaacag aaggattaga caatcaatat caatcaacaa aaatcagcga
     3241 aaaaacaggt aacttgtata tcgtgatcaa tccagatcaa cgtgtagaag atgtatacgg
     3301 ggaatttttc gaataaatcg atttttacgc aaaaatgagg ctgtgaaaga gatgtgtctt
     3361 attactactg ataggctgtc tcttccacac cgtttattca gaataaggga ctgtgacaag
     3421 atttttgtcg cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga
     3481 aagtaaacat ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata
     3541 ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg
     3601 atgaaacaat gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg
     3661 tataggagat ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc
     3721 gctatggcgc cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa
     3781 atatattgat gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa
     3841 aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa
     3901 ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat
     3961 ggacgatgga atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca
     4021 tagctagccg cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg
     4081 tattaggtct gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta
     4141 tgattattgc accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca
     4201 aaaatttcgg gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg
     4261 taggtgcgac gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg
     4321 gagcagcgtt aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata
     4381 tccctaaaaa ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg
     4441 ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg
     4501 cggggatgag tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct
     4561 ggttcaaaca aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt
     4621 atctgctgat cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg
     4681 tatctggaat cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa
     4741 tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt
     4801 ttacactgaa tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc
     4861 cagtatgggg agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct
     4921 ctgtcatgct ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg
     4981 gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa
     5041 agggaacagt cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt
     5101 tttaccagcg gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca
     5161 tagggatcat tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga
     5221 atgctttgat gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg
     5281 atcgtaatac aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga
     5341 tacgtgatct gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac
     5401 aagcactgat tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc
     5461 tttcttctaa tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca
     5521 caagccagat tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa
     5581 tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta
     5641 gtgatgaaga tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg
     5701 aaagtttaga taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc
     5761 aacgcaaact tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc
     5821 agcaagatag tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca
     5881 ttgatgaata cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga
     5941 atatcaattt gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat
     6001 ttgcgt
//
LOCUS       DAYYKN010000151         5980 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000151 DAYYKN010000000
VERSION     DAYYKN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.153"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1006
                     /gene="yidC"
                     /locus_tag="ACHBB7_002548"
     CDS             56..1006
                     /gene="yidC"
                     /locus_tag="ACHBB7_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM9210443.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1185..2159)
                     /gene="lacD"
                     /locus_tag="ACHBB7_002549"
     CDS             complement(1185..2159)
                     /gene="lacD"
                     /locus_tag="ACHBB7_002549"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM9210444.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(2156..2614)
                     /locus_tag="ACHBB7_002550"
     CDS             complement(2156..2614)
                     /locus_tag="ACHBB7_002550"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM9210445.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(2611..4074)
                     /locus_tag="ACHBB7_002551"
     CDS             complement(2611..4074)
                     /locus_tag="ACHBB7_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9210446.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(4056..5006)
                     /gene="pfkB"
                     /locus_tag="ACHBB7_002552"
     CDS             complement(4056..5006)
                     /gene="pfkB"
                     /locus_tag="ACHBB7_002552"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM9210447.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            5149..5898
                     /locus_tag="ACHBB7_002553"
     CDS             5149..5898
                     /locus_tag="ACHBB7_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9210448.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
BASE COUNT         1813 a         1305 c         1034 g         1828 t
ORIGIN      
        1 gcgctttttt agtatagtta agtttgtgta aaaattatag aagaggaagt attctatgaa
       61 taaatggaaa aactggttat tgggatccgg acttgtcagt atcctgctct ttttatctgg
      121 ctgtgtgcgg atggattcaa acggaaaccc tgacacttca ggaatcgttt atcgagtact
      181 cgtccatcct atgggccaag cgattacata tctagttgag aatttcaatt ggtcttacgg
      241 ctgggcagtt attgcaatga ctgtgatcgt tcgtatcatc atcttgccac taggtatcag
      301 tcaatcgaaa aaaacaatga tccaatctga aaaaatgcaa gcgttaaaac cacaagttga
      361 agcagcacag caaaagctaa aagctgcaac aacacgtgaa gaacaaatgg cagcacaagc
      421 agaaatgcag caagtatatc gcgaaaatgg attgagtatg accggcggaa tcggctgtct
      481 gcctctattg atccaaatgc cgatcttctc tgccttgtat tttacggcac gctacacaga
      541 aggtatccgg gaatcatctt tttacggcat cgatctaggc tcaccaagta tggtgcttgt
      601 ggccatcgca ggggtcgctt atctgcttca aggatatatt tcaacaatcg gtattcctga
      661 agaacagaaa aagacgatgc gtacgatgct gatcgtctct cctgctatga tcgtcttcat
      721 gtcaatcagt gcgccagccg gtgttacact ttactgggta gtcggtggtg tgttcagctg
      781 tctgcaaaca ttcatcacaa acgttatgat gaaaccacgt ttgaaagcac aagtggcaga
      841 agagttgaaa caaaacccgc caaaacaagt cgtcactcca agaaaagacg tgacagaatc
      901 agcaaagcca gctaacaaca aacaagcaac aaaacaaatc aaacaaccaa aaaacagtac
      961 aaacaacaaa ggccgcaacg caggaaagca gcagaagcgg aagtagatag taaataagag
     1021 tgtaataaaa attcattaac agttttagca attaaaaata tcgaccaaga tttttgaaaa
     1081 agaaatctgg tcgatatttt tatttaagtg aatgtttaat ttgaatcatc gattgatcgc
     1141 tatctaattg cagcagcatc tttttatgaa gaaacaactt tttcttatct atcaacgtag
     1201 acaggtgttg ctgtttcttc taatagtgta cttaattctt taatattttg ttttccttct
     1261 tctaacatcc aactactcgc tttttcttcg ccttcggtaa caaaaggtgc cactcccttc
     1321 gcccatgttg ctcgcccaca taagacacca ttgaatgtcg aacctgattg ttttgcaaaa
     1381 cgtaatgtat cttgaaatag cgtagcattg actcccgcac ttaaaaaaat aaacggttgt
     1441 tcagtcactt tcgattgttc atagaaatac gcggatgcct ctgcttgact ataacaagtc
     1501 tcttgcccat atccttccac aaatctcata ttcactggca cttctacttt caatacatcc
     1561 acttgataac gttcttttga aaattctcgc atggcttcaa taactttatg tggttttact
     1621 tttgcatagg caagagaaga cggatcgcca atagttgcat cataagaaag aatctctaaa
     1681 aagaaaggta tgccttctgc ttcgcattct gaaccaatcc gttctataac tatttgtttt
     1741 tgcacattaa tctctgctgg ttcatcaacg tcatagtaca ataaaaattt acaagcatcc
     1801 gcaccagctt cttttaacct tttcaccgac caaatattca ataaatcagg taatcttccc
     1861 ggtattttcg tatcatagcc ggttttttca taagctagca gtaagccaca ttctttctct
     1921 cgaagcgctg ctgcaggcag gccgtattct ggatcaagta aaatagctga ggcataggga
     1981 gtcaggtatt gagaaaccat ctgtttaaat tggatgatgt cttctgatgt agcttcttta
     2041 tattggttga tcattttctt caaagcacca cgctggtcga ttgctaatgc gctaataatt
     2101 ccagcttcat ccgtcaatcg cttcaaacgt tcctgttttt ctatactcat cttcttcata
     2161 gatcttcacc tatttcttta gttaatattg tggctatttc tgtttttgat tttgcttttc
     2221 tcaactgttc tgtaacctct tttcgcatca atgatcgtgc aacgagagac aataatttca
     2281 aatgagtcgt tcctgcttct gaatctggaa taaataacgc aaaaataaaa tcaactgact
     2341 gtctatctaa actgttccat tcaattcctt tgttcagtgt ggcgataatc atactaggtt
     2401 cgttgattgt ttgagacttc gcatgaggga tagcaaaacc tgacattatt cctgtagtcc
     2461 cttctttttc tctttctaga agtttttgat atacttcttt ttcgtcaaga gcgatcccta
     2521 aacagaatgt ttgctttgct aaaaacttaa atgcttcttc ttgactatcc aatggctgtt
     2581 gtaaaaaaat gttctcttca tgaatgattg tcatgataag tctcctgctc ttcctttttc
     2641 gataactgta cgatttgctg ctttattatc catcttcttg tctttatcag ttactctttt
     2701 cttttggatt aatcctagta tcactccacc aacgatcgaa ccaataagaa tcgctgtaac
     2761 ccattggatt tttcccgtca ctacaggtaa cactaaaaaa ccaccatgtg gtgctggaac
     2821 ttggacgcca aacaaataag ttaagacagc agcaattgat gagcctaaca tcatcgtggg
     2881 taacactcgg atcgggtctt tcgcagcaaa cggaatagct ccttccgtaa tatgagttga
     2941 tcctaaaata aaattaacca aacctgcatt acgatcattg gtatcaaaat atttaccaaa
     3001 caataaagta gcaaaaccag taatcaacgg tggtgcaata caagctgctg acacaccagc
     3061 cataaaagtc gtgtttcctt gtgccaaaag agctgttcct gttacatatg ccgccttatt
     3121 aaccggtcct cccatatcaa acgcacacat acagccaaca atcacgccaa gaacaagtgg
     3181 attcgaatgt tcaaatccag ctaaaaagct catcattcct tcatttatag ccttcattgg
     3241 acctgccagc aaagacatca cgatgccaat aaatgctaca ccaataactg gatataagaa
     3301 gattgccttc aagccatcta gttgtttagg taaaattgtc agtagctttt gcaaaaataa
     3361 gataaaataa cctgctaaga acccagctaa aatacctcct aaaaatccag taccgccatt
     3421 catggctacg attccaccaa caaatccaac gactaaccct ggacgtttcg cgatcccttc
     3481 tgcaatataa gctgctaaaa ctggaaccat cattcccatt gctgcatcgc caactgcttt
     3541 taccattgcc gctgtagcat tgtattgtgc atgatctgga tcaaaagaat agattcccca
     3601 aacggcaaat gaaatagcaa tcaatactcc gccagctacc acgaaaggca acatatgaga
     3661 gacaccgttc attaaatttt tatagatgct acgaccaaaa ttagcaccac tcatcgcttc
     3721 tggttctaaa gtatcagcag cttcattatc tgctaagaca gtaccttttc ctgccaatgc
     3781 ttcgtcaatc agccgttgtg catccttgat tcctgtactg acagaaacat caatgactcg
     3841 ttttcccgca aatctttctg aatggacatc tttatctgcg gcaataatga cagcatctgc
     3901 ctgtttgatt tcttcagccg ttaattcatt ttcgatccca atttgtccat gggtctccac
     3961 tttgatcttg atgccttttt tcctagctgc ttgttctaat gcctcttgcg ccatgtaagt
     4021 atgcgcaatt cctgtcggac aaccggttgc tgcaattagt tgatattttc ccatgtttca
     4081 aacctctctt ccttgatttt gatttgttta cttaattcct ccacatccaa aaaatcagtc
     4141 agtcctttac gaaaagcggt tgagctgcct gctgctatgc ttttacgtaa cgtttcatca
     4201 agcggtcgtc gattcatata tcctgctaaa aaagcgccta acatggtatc acctgcacat
     4261 gctgtattga caacttttcc ttttggcgag tttcctcgaa acaccctgtc tttcgtaaaa
     4321 agaatcgctc ctttctcacc gagggataac aaaacatttt ctgcacctaa ctctactagt
     4381 cgttgtccat aaacaaagta atcttcatct gtaaccattt ttcgtccgaa ccaagaagaa
     4441 agttcttcct cgttcggttt cagcaaaaac ggtcgatagg gtaaacaatc catggcttct
     4501 tgatcactac tatcaataat caaatgaatc cccttttcct gacaaatccg actgatttct
     4561 attaaaacct ttggagacag ccctcttggt aaactcccag aaacacaaag ataatcttct
     4621 gcttgtaatg ctcttatttg atttaaaaaa ttatggacag ctgtctgcgg tattttaggt
     4681 ccttgattca ctaatttgta ttcttctccc gtttgattga cttgtgtaaa tacattgata
     4741 cgcgtcatac caggcacttc aataaactct gtttggattt gtttctgctg taaatagtct
     4801 tctatatact ttcctgtaaa cccagcagaa aaacctaatg ccgtacttgg gaaatctagc
     4861 atttttaata ctagtgacac attcacccct tttccgtttg cctgaatgtc atcctctttt
     4921 gtgcggttta ccataaaagg atgcatctcc tctgtttcaa taaatagatc aattgccaaa
     4981 ttcatcgtgc atgtgtagat agacataacc aacgacctcc ttgactctat tatgaaatac
     5041 gcgaaagaga aaatgttttc ttaatcgtcc caacacaaag aaaaccttta catttataaa
     5101 ctcgtcgtgc tattttttaa ctaacttttg gtgaaagaag gaaagaatat ggatcacaaa
     5161 ttaagtgaaa cagaacagta cctctggaac tttatcgaac accacatcct agagatccct
     5221 aactactcca tcgtgaaact aagtgaacaa gcaaatgtct caacagccac cattgtgcgg
     5281 acaatgaaaa agaaaggcta tgaaggattt acttcgttta agcaccatct aaaagaagga
     5341 gaaaacaaga acatcaattt ttctttctta gataaagtag acaaaggtat ccgtcgagcc
     5401 attctaaaaa atgaacaaga agtggtacgt acgatcaata tgttggaaat cggaaatatt
     5461 gaggatgcaa tacaaagaat caaatttgct gaccgggttt ttgtctttgc acgaggattt
     5521 tcagaaatga ttggtcaaga gatgctcgta aaacttcaat taacagggaa aaattgtcaa
     5581 atgcatactg atccagaaat catcaaaagc atcagtcgga aactaactaa gaaagacagc
     5641 gttatttttg tttctttgaa cggcgagaca aaagaactcg tcaccgccgc aaagaattgc
     5701 tatgatgcag agattggttc gattctagtt acagccaatc attcctctac actgaaagac
     5761 tattgcgaga tcaatctcgt aggtttcaaa tcagaaggtt cttatttccc tgattatgaa
     5821 gtacggtcac gtttgcctct ccagattata gcgagaatcc tgcttgacgc ctatgtattg
     5881 cgtatggggg agaattgaaa agcagtttat tatggttggt tacagtttaa aacatcacat
     5941 tttttattct ttatattgta tggactcacg gaaacttttt
//
LOCUS       DAYYKN010000152         5926 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000152 DAYYKN010000000
VERSION     DAYYKN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.154"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..1080
                     /locus_tag="ACHBB7_002554"
     CDS             76..1080
                     /locus_tag="ACHBB7_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9210449.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1081..2025
                     /locus_tag="ACHBB7_002555"
     CDS             1081..2025
                     /locus_tag="ACHBB7_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9210450.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2025..2987
                     /gene="opp4B"
                     /locus_tag="ACHBB7_002556"
     CDS             2025..2987
                     /gene="opp4B"
                     /locus_tag="ACHBB7_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM9210451.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3014..3928
                     /locus_tag="ACHBB7_002557"
     CDS             3014..3928
                     /locus_tag="ACHBB7_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9210452.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3954..5735
                     /locus_tag="ACHBB7_002558"
     CDS             3954..5735
                     /locus_tag="ACHBB7_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9210453.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
BASE COUNT         1906 a         1101 c         1287 g         1632 t
ORIGIN      
        1 aaaacgcttt attctgtgta atgtcctttt ttattaagaa aaacttccat ttaggaagca
       61 gagaggggta ttcgattgtc cgataaccaa ttattagaag ttcagaacct acatacagga
      121 ttccgactca aagatgacta ttacgatgca gtagatgacg tttcatttac tttagacaaa
      181 aacgagattt tagcaatcgt tggggaatct gggtgcggga aaagtacctt ggctactacg
      241 attatgggat tacttgatcc taataacaca aaaattacag gtgagatttt atataacgat
      301 ctgaatttaa tcgacctgaa cgaaacgctc tataataaaa tccgcggaaa tgatatcgga
      361 atgattttcc aagatccgtt gtctgcatta aatcctctca tgagaatcga agaccagatc
      421 aaagaaggac tttcctatca cacaaagatg aatgcagaac agcgtcaagc tcgtgcacta
      481 gaattgctag aacaagtagg gattcctaat cctgcgagag ttggacgcca atatccccat
      541 gaactatcag gcggtatgag acaacgtgtc atcatcgcaa tcgcaatcgc ctgcaaacct
      601 ccaatcgtca ttgcagatga accgacgaca gccctagatg taaccatcca agcacagatt
      661 cttgatttac tgaaagacct tcaggaagaa acaaaaacag ggatcatctt gatcactcat
      721 gacttaggag tcgtagcaga aatggctgat cgagtggcag tgatgtatgg cggacagttc
      781 gttgaagtag caggtgtaaa agaactgttt gatcatccta agcatcctta tacacgctct
      841 ttactgaact ctataccaca agaaggaaat catgatgcag aactccatgt gatcgaaggt
      901 gtcgttcctt ctctgaaaaa catgcctcgt aaaggttgtc ggtttgcacc acgtattcca
      961 tggataccag caagcgatca tgaagaagag cctatccttc atgaagtcgc accgaaccac
     1021 ttggtacgtt gctcctgcta taagcatttc cactttagag acgaaaaagg agaggtgtag
     1081 atgacagaat taatcacaat caaagacctg aaagttcatt accctattcg cagtggattc
     1141 ttcaatcgag taacggatca tgtatatgct gtagatggtg tggattttat tatcgaaaaa
     1201 ggaaagacat atggtttagt gggagagtct ggctctggaa aatctactac gggaaaagcc
     1261 gttgtgggat tagaaaaggt aacagccgga caaatcatgt atgaaggaaa agatgtaacg
     1321 aagcgaagca accgcaaaaa aatgggttat aacaaggatg tgcagatgat cttccaagat
     1381 tcaatgtcca gtttgaatcc taaaaagcga gtgttggata tcattgctga acccatccga
     1441 aattttgaac ggttaagcga tcaagaagaa aagaaaaagg taaaaggact gctagatatc
     1501 gttgggatgc cggaagatgc gctatataaa tatcctcatg aattttcagg cggacaacgt
     1561 caacgtctag gagtagcccg tgctgttgct accaatccaa aactgatcgt agccgatgaa
     1621 cctgtatctg ctctggatct atctgttcaa gcgcaagtat tgaatttcat gaaacgaatc
     1681 cagcaagaat acggcctcag ctatttattc atttcacatg atttaggtgt agtaaaacac
     1741 atgtgcgaca atatcgcaat tatgtacaaa ggtcgatttg tagaaatcgg tacaagagaa
     1801 gacatttata atgacccgcg acatatctat acgaaacgtc tgctttcagc tattccgcaa
     1861 atcgatgtag aaaataggga aagccacaaa gaaaatcgac gaagagtaga acaagagtat
     1921 atccaaaacc aaaaagaata ctatgatgcg actggtcgag tctacgattt gcgtacgatc
     1981 acaactacac acaaagtggc gttgaaagat ggaggtgcaa gctaatgtgg aaaacgattc
     2041 tgcgacgtgt tttattgatg atcccgcaag ttattatttt aagtgttctg atcttcttat
     2101 tagcccaagc gatgccgggg gatccattta ccggtttgat caatccaaat caggatccgg
     2161 cagtgatcga acagatgcga caagcagctg gcttgaatga cccatggtat gtccagtact
     2221 tccgttggat cggaaatgct ttgcaaggcg attttggtca aagtttctta tacaaacgct
     2281 ctgttgctga cttgatcggc gaacggatca tcaacacttt atatctatcg atcttgacag
     2341 ttgtccttac ttatttgatt gctgtgcctc tggggatgct tgctggccgg tatcaaaatt
     2401 caatcttgga taagattgtc gtaatctaca acttctttag ttttgctgtt cctttattta
     2461 tcttcggctt gatcatgcta tttgtttttg gttatcgttt aggctggttc ccaacaagtg
     2521 gatcacaaac actaggtttg caaggtggat ttatcactca atggatagac cgtttgaaat
     2581 atattctgtt accatcaatt acccaagcat ttttggcgac tgccgtcaca atccaatatc
     2641 tgcgaagtga agtaatcgat tcgaaatcgc tcgattttgt cagaacagcc cgttccaaag
     2701 gggtaccgac gaataaagta ttcagtcgac atattttccg aaatgcagcg ttgccgatgg
     2761 cttctcaaat ggggtatgaa atcacttcgt tgattgctgg ttctgtagtt atcgaaaaga
     2821 tttttggtta tccgggagtc ggaaaactat ttattgattc catcggccaa cgggactata
     2881 ccgtgatcac atccttagta ttgattttag gaatcgcgac gctagtgggg acattacttt
     2941 cagatatcat catgagtatc gttgacccgc gtattcggat tcaataaaac caatcagaaa
     3001 ggagaatgga tcgatgagtg aaaaaaacaa tacagcagtt caagaaagta ttcctcctat
     3061 ggggatacga atgattgctc gagaatttaa aaaagataaa attgctattt tttcattagg
     3121 tttgctagtg gttttattat tagtgatctt tatcggtgcg atgctgaccg atcaagacaa
     3181 agtgatgtat gttagtatct tcgacaaata cgctgcacca ggaacagttt cgacacaagg
     3241 acaaaagttc attctgggag cagatgaagg cggacgtgat gtattaggac aattgatcat
     3301 aggtgctaga aactctgtta cgatcggatt tgcgatcacg atccttactt cgatcatcgg
     3361 ggttggatta gggatcatct ctggctacta cggagggatc attgacaaca tcttgatgcg
     3421 gatcgttgat tttatcatga ttctaccgat catgctgatc attatcgtct tcgtatcgat
     3481 tatttccaga tacaatgtct ggtcctttat attcatcatg agtgcatttt attgggtagc
     3541 taaagcccga ttattccgaa gcaagacatt atctgaagca agaagagatt atgtcagtgc
     3601 ctcaaaaaca atgggaacca atgattttaa aatcatgttc cgcgaaatca tgccgaatct
     3661 aagttcatta atcattacta atttgacgat gaactttgcg gcaaacatcg gtatcgagac
     3721 aactttgact ttcttaggtt tcggcttgcc ggcaaatgta cctagtttag gtacattgat
     3781 cggttatgca agtaacggag aagtcttagt gaatcggcaa tggatctggt tacccgcatc
     3841 aatccttatt ttagttttga tgttgagtat caattatgtt ggacaagcat tcaagcgttc
     3901 tgcagatgcg cgccaacggt taggctagta tagaagcgaa gggggaaata ggaatgaaga
     3961 aaacgatttt tggatttgca gcgctgatgt ctgctgtcgt tcttgcagca tgcgggggcg
     4021 gaggtacaga tacctctgga aacaactctg gaggcaacgg caataacaaa gaggaattaa
     4081 cttttccaat tgcaacaaaa aataatgatg atgcaatcga agacggcgaa ttagaagctg
     4141 cggtcgcaac ggacagtcag tttaaaggat tgttccagtg ggagttttac caagattcat
     4201 atgatgcagc ttttatggct ccttctcatg aaccgctatt tgcaaacgat gacgatttca
     4261 gaatcacaga cgacggagct gcaacgcttg atctagatga agatacgaaa aaagcgacaa
     4321 tcaaagtaaa agacaacgtc aaatggtcag atggtgaaga tgtaacggcg gacgatgtga
     4381 ttttccctta cgaaatcatt gggaataaag attacactgg tatccgttat gacgacactt
     4441 tccgtaacat cgtaggtatg gaagaatatc atgatggaag tgccgacaca atctccggaa
     4501 tcaaaaaaat agacgacaaa acagttgaaa tcgaatataa agaagtaaac ccaagcatgc
     4561 ttcaatcggg tggtggtatc tggtcatatg cagcaccaaa acatacgtta gaaggtgtag
     4621 caatcaaaga tatggaatca agcgatcaag tacgtaaaaa tccagtgact tttggccctt
     4681 attacatgag taatatcgtt gcaggagaat cagtagaata cttgccaaac gagcactact
     4741 ggaatggtac gcctaaattg aaaaagatca cgatgaaatc tgtaccgatg gcaagtgcaa
     4801 ctgaagcatt gaactctaaa ttatatgata tgattttcca aatgccaaca gatacttacg
     4861 atacctataa agaaattgct ggctatacaa acttaggccg tgaacaaact tcttatactt
     4921 atctaggatt caagttaggt aaatggaatg cagatacaaa gagtgtggaa tacaatccag
     4981 attcaaaaat ggctgataaa tcattacgtc aagcaatggg ctatgcatta gataataatg
     5041 cagtaggtga acgtttctat gcaggtttga gaagcaatgc gacttcattg attcctccag
     5101 tattccagtc ataccatgat gatgagatga aaggattcac acaagatcta gacaaagcga
     5161 aaaaactatt agatgatgct ggctacaaag atgtggatga cgatggttta agagaagaca
     5221 aagatggaaa taaactaaca atcaactttg cttcgatgtc cggtggggaa acagcgcaac
     5281 cattggcaga ttactatatc caacaatgga aagaaattgg tttggatgtc caatacacaa
     5341 caggtcgtct gatcgaattc aattctttct atgataaatt agaaaacgat gatcctgaaa
     5401 ttgatgttta ccaagctgct tggaatacag gaactgatcc aaatccaagt ggactatggg
     5461 gaccaaatgc tgcgttcaac tacactcgtt atgaatcaga agaaaataca aaattgatca
     5521 acgatatcag ttcaagcaaa gcatttgatg aagagtacca aaaagaagca tatcaaaaat
     5581 ggcaacaata tgcatttgaa gaagcatttg cgattccaac gttgttccgt aatgaagtat
     5641 tgccagtcaa cgaccgtgtc aaagactggg attggtctta tgatgtacca aacccatggg
     5701 caaatgtttc tgttacagca gattcacgtt cataatcact aaaaatgacc gaaaaagggc
     5761 gatgtccttt ttcggttttt tgatattttt ctgtttattt ctctaatatt caggtcaagg
     5821 cgctgatttc atgacttctc caataaacgg tgttcaaagt ccagtttctc ggtattaagt
     5881 caaatttatg caaaaatatg gaaagccatt ttcacaaaat tttttc
//
LOCUS       DAYYKN010000153         5899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000153 DAYYKN010000000
VERSION     DAYYKN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.155"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..767
                     /locus_tag="ACHBB7_002559"
     CDS             <1..767
                     /locus_tag="ACHBB7_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HFM9210454.1"
                     /translation="TDILTEYSKEHQQIFLHFWQYDCTVILGMKDVRTPFLSDGIQTL
                     KDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTKKITIDDAYEQMLLLTKYAFPKA
                     PIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNGISVMMYLSVNGNQEKRGQVMKR
                     FYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFVKEVKEAFAKAFEKLYSASDLQD
                     STDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL"
     gene            751..2118
                     /locus_tag="ACHBB7_002560"
     CDS             751..2118
                     /locus_tag="ACHBB7_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9210455.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            2137..2946
                     /gene="yidA"
                     /locus_tag="ACHBB7_002561"
     CDS             2137..2946
                     /gene="yidA"
                     /locus_tag="ACHBB7_002561"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM9210456.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(2989..3432)
                     /locus_tag="ACHBB7_002562"
     CDS             complement(2989..3432)
                     /locus_tag="ACHBB7_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM9210457.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            3566..3937
                     /locus_tag="ACHBB7_002563"
     CDS             3566..3937
                     /locus_tag="ACHBB7_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9210458.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(4106..4888)
                     /locus_tag="ACHBB7_002564"
     CDS             complement(4106..4888)
                     /locus_tag="ACHBB7_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM9210459.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(4885..5856)
                     /locus_tag="ACHBB7_002565"
     CDS             complement(4885..5856)
                     /locus_tag="ACHBB7_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM9210460.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
BASE COUNT         1846 a         1191 c         1053 g         1809 t
ORIGIN      
        1 taacagatat attaacggaa tattctaaag aacaccagca aatcttttta catttctggc
       61 aatatgactg cacagtcatc ttaggtatga aggatgttcg aactcctttt ctaagtgacg
      121 ggatccaaac actgaaagat gcaggttacg ctccagtgat cagaaattca ggtggattag
      181 ggatcgtatc agatgaagga attttgaata tttctttgat tttcccccaa agtgacacaa
      241 aaaaaataac catagatgac gcttacgagc aaatgctcct attgactaaa tacgcttttc
      301 caaaggcacc gatacaagca ttcgaaatca cccattctta ttgtcctgga acttatgatt
      361 taagcatcca tggcaaaaaa tttgccggta tcgctcagcg tcggatcaaa aatgggattt
      421 ctgttatgat gtacttaagt gtcaacggta atcaggaaaa acgcgggcaa gtcatgaagc
      481 gattttatca atctgcttta aaagaagact ttggtacaaa tggctatcct gcagtggatc
      541 ctgctacaat ggcaacactt gaagaactgc ttgaccaacc cctttttgta aaagaagtca
      601 aagaagcatt tgcaaaagct tttgaaaagc tgtattctgc aagtgattta caggattcta
      661 ccgactggct aaaaaaagaa atccagctgg agcaatggaa caaccaaatc cagagaatgg
      721 aacaacgtaa taacataaag gagctacatt atgacaaccc cttataaata tcaaatgctg
      781 cctatggaaa aagtttttcg tgatccagtc cataactata tccatgtcca gcatcaagta
      841 atcttagatt tgatcaattc gaaagaagtc caacgtctac gacgcattaa acaattaggc
      901 acttcttctt tcacttttca cggagcagaa catagccgtt ttactcattc attaggcgtt
      961 tatgagattt caagacgaat ctgcgatatc ttttctcgaa atttttcaaa agaaaaaata
     1021 ggcaacggag gctgggatga tcaggaacgg ctggtgactt tatgtgcggc actgttgcat
     1081 gatgtcggtc atggtcctta ctctcataca tttgaacata tcttccatac gaatcatgaa
     1141 gccatcactg tagagatcat cacttctcca gaaacagaag tgtatcagat tcttaaccga
     1201 gtagaagaag gttttcctga aaaggtagct agtgtgatcc aaaaaactta ccccaaccca
     1261 caagttgtcc aaatgatttc tagtcagatc gatgcagatc gaatggacta tttgcttcgg
     1321 gatgcttatt tcactggaac agaatatgga acatttgatc ttacccgaat attacgtgtg
     1381 attcgtcctt atgaaggcgg gatcgctttt tccatgagtg gcatgcatgc agtggaagat
     1441 tatatcgtta gccgatatca aatgtatgtg caagtgtact tccatcctgt ttcccgtggt
     1501 atggaagtaa ttttggagca tctgcttcat cgcgcacacg agttgtatca agaagatgcc
     1561 agcagttttg aactccattc tcaattactt ctgccatttc ttgacggaag ctttacttta
     1621 gaagaatacc tcaaacttga tgatggggtc ttgggcactt actttactca atggtctgaa
     1681 tctgctgatc ctattttaaa tgatttagct aaacgttttt tgaatcgtaa accactgaaa
     1741 tctgctactt tttctggcaa ccgggattca aaattagtcc aagaactcac tttgttagta
     1801 gaaaaggtcg gatataatcc tgtttattac actgctgtga attctagcta cgatctacct
     1861 tatgattttt atcgcccaga acaaggcaga catcgcacac aaatcgagat tttgcgaaat
     1921 gacggaactt tgatagaatt atcacaggtt agtcagcttg ttgctgcatt agcgggacaa
     1981 gaacaaggag atgagcgatt tttctttcca aaagaaatgg ttgatccgtc cttgcgtgat
     2041 cattatgatt tatttgacga aacttatcaa gaatttgcca gccatatccg taatggcgca
     2101 ttgattgaaa taaattaaaa tatattggag gaaattatgt ctatcaaact agtcgctatc
     2161 gacatcgatg gtactttatt aaatgagaaa agagaagtaa caaaagaagt aaaagaagcg
     2221 attgccgctg ctgttgcaaa aggtgtttcg atcgttttat gcactggccg tcccttacca
     2281 ggtgtccagg aacagttaaa cgagttgaac ctttttcaag ataatgatta cgttattact
     2341 tataacgggg cactcgtaca acaaacgaaa tcaggtaaaa tcatcgcccg tcacggactg
     2401 acacatgaag atttcctaga gatcgaagtg atggcacgcc gtgtcggcag tcatctccat
     2461 tcgatcgatg acaaagcgat ctacacgcct aatcgagata tcagcgctta tacgatccac
     2521 gaagcaagtt tagtcaagat gccgctaaaa tatcgaacac ctgaagaaat gacaccagac
     2581 atgaacatcg tcaaaatgat gatgatcgac gaaccagaaa ttttagatgc agcaattgcg
     2641 cgtttaccgc aaactttccg tgataaatac acaacggtca aaagtgcgcc ctattacttc
     2701 gaagtactaa ataaagaagc aagtaaaggt gcggcagttg cgaatctcgc acagcacctt
     2761 ggtattgatc aagatgaaat catggctatc ggagataacg agaacgactt atccatgatt
     2821 gaatatgctg gtctaggagt agcaatggga aatgccgttt ctcttgtcaa agaagcagca
     2881 aatgtcatca cttcatcaaa cgacgagcac ggtgtagctg aagcaatcaa aaaatatgta
     2941 ctgtaataaa taaaaaaact gagagaaagc tgatctactc gctttctttc agtttttatt
     3001 tttttcgatc catctctcga ctgcccatcg gtggctacca cgtttcaatt tcacaagcgc
     3061 ttcttgaggg ctcacccacc aaattttatt ggtcttttca gttggttctc ctatacgctt
     3121 ccaagaatca gcaacaaaga aatatcctgg attataataa taagttgcac gataattcga
     3181 gtaaaaatac tcatcagctt ctcccagata agaagaaatt tctgccacaa ttcccatttc
     3241 ttctatcagc tcacggacaa tcgcttcttc tttcgtctct tctccttcaa tttccccgcc
     3301 aggaagaaaa aaagcaccat ttggagcctc gattactgca atctgctgat ctctttctat
     3361 gacaatatat gctgcataac gggtttgata atcagcaaat tgcttttttt caccaaaaat
     3421 tggtttatcc atgaatagtt tccaccttaa cagttttatt ttctatccat tttacatgag
     3481 aaatttctca tgaacaagca caaatcaact atttccttac ttctttttgt tatgattgag
     3541 taaaaggaaa aggagagatg aatctatgaa aatggctcac acatgtgttc gagtgaaaga
     3601 tcttgaagca tcaatcgaat tttatcaaaa ggcatttggt tttgaagaaa gccgccgccg
     3661 tgattttcca gaaaacaaat ttacacttgt ttatttgaca cttcctgggg atgactatga
     3721 attagagttg acttacaact atgatcatgg tgcgtatgat ctaggagacg gctatggtca
     3781 tatcgctata gcagctgatg atctagaaaa attacacgag caacataaag ctgctggtct
     3841 tgagattact gacttaaaag gattacctgg cactgcgcct tcttactact ttgtcattga
     3901 ccctgatggt tataaaatcg aagtcatccg aggatagtta gtatggggca ttttactctt
     3961 gattgaataa aaaacaaaca ccaataatta aaatgagacg caaacttttc attaaattga
     4021 aaaagtttgc gtcttttttt aacaaccatt gtagctataa ggttgccact ctccaccaat
     4081 ttatttttaa ttcatttgaa agaaatcaaa taaacgaggt acctttagaa aacaaaactg
     4141 cttttcctcc tattatcaca cgatcttctt ttaattcaca ttctagatcc ccgccccttg
     4201 atgacatttg ataagctgac aattttgttt tattcattct ttctgcccaa taaggaataa
     4261 gattagaatg tgctgatcca catactggat cttcattgat tgttaatttt ggaaagaagg
     4321 ttctagaaac aaagtctttt tgcacacttt ctgctgtcac gatgactcct attcctaatt
     4381 caaaattttt gatagctgta aaatctggct gcaatttagc caccgttttt tcatcttcta
     4441 atacgaaaaa taaatcacgt gctaagtagg cttctttaat cttagcccca atcgcttctt
     4501 catattcaga caatatcggc acttttttcg gcataatact aggaaaatcc atgtaataat
     4561 aatctttaat tcttgtcaca aatagattac cgctttgagt agaaaattta atcgtttcat
     4621 caggtatttt gtaatagtta aataaaacaa atgctgttgc taatgtcgcg tgaccacaaa
     4681 gatcgatttc tcgatctggc gtaaaccatc taagctcaaa ttcttggttt ttcttcactg
     4741 taaatgctgt ttcagataaa ttgttttcaa tggcaatttt ttgcatcgta ccttctggca
     4801 gccattcttc tagcacataa accgctgctg gatttccttt gaacacctca tctgcaaatg
     4861 catcaactac gtaataatct aagttcattt taaagcccct ttctctaatt tgttataaat
     4921 atattctgca caacaaagat cttcaatagc tagaccaact gcgtcgaaaa tcgtcggcct
     4981 cgattctcct ttataaaatg tttttttctc agaaactaat tcgcctaatg taccaacaat
     5041 acaagattcc tctaaatctc cttctgccat ggctataagc aggtccccgc tttctgttaa
     5101 ggcagctgag taatcatcta catagatttg agaagtttcc atcaacgatg tacttatctc
     5161 tcttgtatca ggagtaaatg tgcccacagc attgatatgt gcgtcttccc ttacccactc
     5221 tttttgaaga aatgcctctt tactagaggt caacgtacaa atgatttccg ctggacgtac
     5281 tgcatcttct actgatattg catttataaa cgttatttta ggatactgtt ttttagcttt
     5341 agcgataaaa gtatttcgtc tttcttctaa aatatcgtaa acaaacactg tagtgatggg
     5401 acgaatggta gaaatcgcag tcaaatgcga caatgcttgt tgaccagctc caattaaagc
     5461 aagtgtttct gcatgcggat ttgctaagta atctgttgcc agcgcagata cagcagctgt
     5521 tcgaatccag gtgactgcat ttgcgtttat caatgctttg ggtagtccgt tttgagcatc
     5581 aaacaagaaa atctctccta aatgagaagg taaatgatac tgatgattat ccggaaaagc
     5641 ggtaatgact tttgtgccaa aatatctgtt ctcccccaaa taagcaggca tcatcgcaaa
     5701 catatttttg ccttttccgt caggtaaaat ttccaccaat cgaggatttt ggctgatttt
     5761 accactttca taatcaaaaa aacatttttt cattacttga atcgcttctt caaaactaaa
     5821 agaagctact acttcctcaa aattaataat tttcatagac aattccctcc tcacaaaaga
     5881 ttaacttcta aaatcaata
//
LOCUS       DAYYKN010000154         5808 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000154 DAYYKN010000000
VERSION     DAYYKN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.156"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACHBB7_002566"
     CDS             complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACHBB7_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM9210461.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2240..4426)
                     /locus_tag="ACHBB7_002567"
     CDS             complement(2240..4426)
                     /locus_tag="ACHBB7_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9210462.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACHBB7_002568"
     CDS             complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACHBB7_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM9210463.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4648..5088)
                     /locus_tag="ACHBB7_002569"
     CDS             complement(4648..5088)
                     /locus_tag="ACHBB7_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM9210464.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5162..5701)
                     /locus_tag="ACHBB7_002570"
     CDS             complement(5162..5701)
                     /locus_tag="ACHBB7_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM9210465.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1503 a         1305 c         1015 g         1985 t
ORIGIN      
        1 tcgaaaaaaa ggttgtgaca cgttttttgt cacaaccttt tttcattatt atctgatttt
       61 caatgtcaaa gcatttagag ctacaactac cgtacttaac gacatcaaga cagcaccaac
      121 tgctggactt aatatgatac caattggcgc aagaattccg gctgctagcg gaattgcaag
      181 aatattgtaa cctgcccccc accagaggtt ctggatcatt ttctttcgcg tttgcttggc
      241 tagatcaaga aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga catccgcaga
      301 atcgattgcg atatctgtcc ctgcaccgat tgccatcccg attgaagcac gggctaaact
      361 tggcgcatca ttgatcccat cacccaccat gattactttt ttcccttggt caaggtattt
      421 ctggacaaca gcttctttat catctggtaa taaaccgcca taatattctt cgatccctaa
      481 ataatctgct actgcttggg cagcgttttt attgtcacca gtaagcatga caggagtgat
      541 ttttctttct ttcaatgttc ggataaattc ttttgcttct gtttttacta cgtcacctaa
      601 agcaacgatt gcagctagat gattttctaa aatcaggaag ctgatggtat ttccttgttc
      661 ttggtaaggt ttcaataatg cttgttctac gtttaatccg agacgctcaa cttctttttc
      721 attaactatt ttcacttgtt gttttttgac tgttgcctct agcccgactc ctgaaagatt
      781 ttttagattt tcagcttgat aaggcttgat ttcatgttct ttcaaataat tcataatgcc
      841 aacagccaat ggatgattgg cattcgcttc caaggctcct agatattgaa ggatttcatt
      901 tttattgaac gcatcatcaa gtacttcgac acccgttaca gtaaattttc cttctgttag
      961 tgttcctgtt ttgtccagca tgatcacatc tagatcattg gcttgctcca gtgcattgcg
     1021 gtttttcaaa agtaatccgt ttttagctgc tattgatgtg gaacgagcaa caactaatgg
     1081 gatagctaaa cctagagcat gaggacaggc gatgataaag actgttacca tacgttctaa
     1141 tgcattagga agatctgtca agaacaacca ggcgatgaat gccaggatac ccacaattaa
     1201 tgcgacataa aacagccatt ttgccacttt gtcagataat gactctagct tggatttttc
     1261 tccttgtgct tttcgcacca tttccataac cttcgctaga taaccatttt caccagttcc
     1321 agttacttcg atttcgatcg ttccatcccc attgatagaa ccgccaatca ctttgtcatt
     1381 ggcttgtttt ttgattcctc ttgattcgcc agtcacagct gattcgtcaa caattgtttc
     1441 acctttcaag attacaccat ctgttggcat tttatcacca gaccggacga tcaaacgatc
     1501 accttcgttt acttctttca atgaaactgt ctcttctttt ccatctttcg tcaagcgttt
     1561 cactgattct ggcagtaatt ctgctagttt ttgtaaagca ttgctggcat tcgacacagc
     1621 attcatttca atccagtgac ctagtagcat aataacgatc aaagtagcca attcccagaa
     1681 gaaatccatg acgtgtgtat gtggattgag cagatttgcg ataaacgaat agacactata
     1741 aatatatgca actgtaatcc ccatcgcgat cagtgtcatc atagcaggac ttttttgttt
     1801 caattccatc ttagctccac ttaaaaatgg ctgaccgcca tagataaata gtatagttgc
     1861 taagatcaat acgagccaat cagaaccagg aaacgttact tgaaatggaa attccattcc
     1921 catcattggt gaaaaaagaa tgattgggat agctaggata agcgatagcc aaaatttttg
     1981 tttgaaattc cccatgtgca tggaatgatc catgccgccg tgttcgtgat ccatgtgcat
     2041 cgaatggtcc atttgtttgc tgtttgctat atctccatgg ttatgtccca tgtgcatgga
     2101 atgatccatc tgactatggt catgtttcat ctccatctgt ttttcatgag attctgtact
     2161 ttctttgttt actacttctt ttttatgctc atgttttttc tccatatcgt tcccattcat
     2221 ttttactcca tcccttctct tactttttca tgtgtcgatt taagcacagg gagttcaaca
     2281 atacactgac ggaactaaac gccatcgctc ctcctgcaat gattggattc aaaaagccta
     2341 gtgcggcaaa aggaatacca atcgtattat agacaaaagc ccagaaaaga ttttgtttga
     2401 tttttctcaa tgtcaaccta gacaactcga ttgtttgtgc aatggatgcc aaactgctat
     2461 tcatcagcgt cacatcggct gtttccatcg caatgtccgt tccacttccc attgccatac
     2521 cgacatcagc taatgctaag gccggtgcat cattgattcc atcgcctgcc ataccgactt
     2581 ttttgccatc tttctgaagt ttttctacgt aggctgcttt atcctctgga agtacttcag
     2641 caaagatatg ctttggatca ataccaactt gtttaccaat cgtttcggct gccagtttgt
     2701 tgtctcctgt cagcataaaa acatctaatc ctttattctg gagctgcttg attgcttgtt
     2761 tgacttctag tttgatttga tcagccacgg caatcaaccc gatcacttgc ttttcatcag
     2821 ctaaaaacat gactgttttt ccctgctgtt ctaattctaa cgcgtgttct tggtattcat
     2881 caaatgatag gttcaattca attaatcgtt tacgtgtacc cgcaaaatag cgtactccgt
     2941 taatcgtacc gctgattcca gcacctgggt gggctgtaaa atcaatcata tcgtatgttg
     3001 ctgcttcttg cagttttccg tattccacga ttgctttacc tagtggatgt tcagaagctt
     3061 gttctaaagt ataaaaaata gataggactt ctttagaacc gactagatct gtaactttgg
     3121 gctttccttc tgtaatcgtt ccagttttat ccaaaacaat gctgtctaag tgagcagctg
     3181 cttctaatgc ttctccgcct ttaattaaaa ttccatttcg tgcaccaaca cccgttccta
     3241 ccataatagc tgttggagtc gctaaaccta atgcacatgg acacgcaatg actaatacag
     3301 agacactatg gatcaatgcg agttgccaat caccagtgat caaacctgta gctatgagcg
     3361 tgataaacgc gatgactaat acgatcggaa caaaaatccc tgagatccga tctgcgattt
     3421 tttggattgg ggctttgctc ccttgagcat cttccaccat ttgaatgatt tgcgctaata
     3481 ctgtctgctt gccgacttgt gtaacttcca tgtgaagtag accattcgta ttgattgtcc
     3541 cgccaaagag cgtgtcatcc ggattctttt ctacaggaag attttcacca gtaagcatac
     3601 tttcatctat cgcactgctt cctgaaataa ttcgaccatc tgctggaatc tgctcaccag
     3661 gacggatgac tagttgatca ccaacgacta cttcttcaat cgctaacgtt tcttctttcc
     3721 cgtttcttat cacttgggcg gtttttgttt gaagagacat caattgtttg attgcattac
     3781 ctgttttcgt ttttgctgta tgttccaaat atttgccgag caaaatcagc gtgatgatca
     3841 tactgctgct ttcaaaataa agatcttggg ggtgtccacg gaaaaatccg ttgtaaatgc
     3901 tcaatgcaaa ggcagccgat gttccaattg ctaccaatac atccatattt ggtgctttcg
     3961 tcttgattgc atggtacgca cctttataaa aacgagcacc gacataaaat tggacaggag
     4021 cagacagcac taactggacg attggaaaat gaaaaaagtg aacaatcgcc gcatggcttc
     4081 ctaacatcat agcaatcatt gacaacatca acggcaaagt caaaaccgta ctcaatataa
     4141 gatcgaatag catttttttc aaatacgctt gcttttcttc tgcaattttt tgtttgtggg
     4201 cttcatcgta caaaattgca ccatagccga tgttttctac gctttggatt aatttttcag
     4261 tggtcgtccc ttcatatttg acacttgctt tttcagtggc caagttgact gtggcattga
     4321 ccacaccagg ttgtccgttc agctcttttt cgatcctagc agaacaattt gcacaagtca
     4381 tacctgtaat gacgaacgta tccattttgg tttctgctgt tgccatcaaa tcacctctgc
     4441 tttataaccc aatccattga ttgcttgaca aatcttttcg gattgaactt ttgtttcatc
     4501 aaacttgacc gtaccgtttg cttttttcaa atttactttc actttttgca cgccgtcaag
     4561 tgcgcttact gtttcttcta ctcttgctgc acaatgattg cagctcatac cttcgattga
     4621 aaattgttgt ttcatttttt cttcctccta ttgatgttgt ttacattcac attgacctgg
     4681 gatacagtta cattcgatcg tatctactgg ttgcttttgt tccagttgtt tcaaaatctc
     4741 attgatatct tcttgagtca aagtggcttc ttctactaga tccgcaatcg tctctccaat
     4801 cctttttgca cagatatggg aaaaaagatt ttccgtcgca ctgcgaactg tttccatctc
     4861 cgagacagct ggataataaa tgaatttttt tccttcttgt tctgtccaga tcacttcttt
     4921 ttttaccaaa cgacccagca aagttttgat cgttgctggt ttccaatcca tcgaatcagc
     4981 taagatctgc gtgatttctt gggctgtcgt ctgtccaagt gtccaaataa cacgcatgat
     5041 ctcccactct gaatcactga tctttactgg cgttttgact ttttccattt atttcacacc
     5101 tttcgtttac aaatgtaatc attatcttat gaatacagat tacacttgta aacaaaaact
     5161 gtcaattatt tttgctctct attttcaatt tagttaaaaa ttcttgtttc tctcgtcttg
     5221 ttttaaggat gatcagttta tttcttgcat tagctacttg gatcagctgc tgtattttcg
     5281 gttcattgtt ttcttcaaaa tgccagataa aagataaaaa ttctaagtat tccctgttta
     5341 gtttctccga aaatgattca ggcatatctg gccgagattt tttgtttatc tttcttttta
     5401 gagaacgcca aactactcgg tagatggcga gataacgcgg cactttaagc caaatgatct
     5461 tatctgcttc tttaatcctt tcctctaatg tagaagtata atttccttca acgatccatg
     5521 acggattaga taccataaac aattgctgtt cttgcaaaaa ccactttttt gcctgaatac
     5581 tgtagtctgt tgtatgccat aaaaggtcta gcggcagtag cggaaagtcg gtgatttctt
     5641 tgatcttcct tgcgagggtc gattttcccg acccactttg tccaatcacg atgatcttca
     5701 ttcttctccc tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa
     5761 aaaacaaggg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaa
//
LOCUS       DAYYKN010000155         5774 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000155 DAYYKN010000000
VERSION     DAYYKN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.157"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..675
                     /locus_tag="ACHBB7_002571"
     CDS             <1..675
                     /locus_tag="ACHBB7_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HFM9210466.1"
                     /translation="YEFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILE
                     LLDGYNSDLISEYKNEIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKL
                     SHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEH
                     RGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVK
                     SYLKMN"
     gene            678..1574
                     /locus_tag="ACHBB7_002572"
     CDS             678..1574
                     /locus_tag="ACHBB7_002572"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9210467.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1624..2013
                     /locus_tag="ACHBB7_002573"
     CDS             1624..2013
                     /locus_tag="ACHBB7_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9210468.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            2000..4330
                     /locus_tag="ACHBB7_002574"
                     /pseudo
     CDS             2000..4330
                     /locus_tag="ACHBB7_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4438..5625
                     /locus_tag="ACHBB7_002575"
     CDS             4438..5625
                     /locus_tag="ACHBB7_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM9210469.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         1953 a          916 c         1219 g         1686 t
ORIGIN      
        1 tatgaattta cacaagagaa tggtctggtt gaaaaagaaa cagaacggtt gagaatgatt
       61 tccgataagg tgagtatata ctctcaaaaa caaatttatg aaatttcaaa aaatcaaact
      121 agtatactag aattactaga tggatataac tctgatttga tttcagagta taaaaatgag
      181 attgaaacat gtgtaaacga aataagaaaa ttaaattggg atattgtttc tgtgaaaaaa
      241 gaaatacaag ataaggcgaa ggtagagctt gaaattgaag atttaaaaaa acaagtagaa
      301 aagctatctc ataagagtta taaagatgtt tatgatgagt attcaaaaga gacagatatt
      361 tatagggaac tgaaacgtga tgtggaggca cttaaggaaa ttcccaaaaa tctaactgat
      421 agtgttgaca aaattgactt tggcaatggt ataaaagtta cagacgaaat tgatgaacac
      481 cgcggagaat tgattaagaa tttagtgacc aatcgggcta agattcaaac aataattaat
      541 gaaatgtcag atgaaattga tagttataga gcagagctga ataaatcaga ttggaaagtt
      601 aactataatg aggtttcaaa gaggtatcaa agaggtatca aaaacttagt gaagagttat
      661 ctcaagatga attaaaaatg ttacaaaacc ttgattcttt atatcaatct ttatcaaaaa
      721 ctgaagagcg attggaagaa attattcaac aagaaataaa gcttaaagat taccaagttg
      781 aggttgactc agcaatgtca agaatgaata aatcatatca aaatctctat tctgagagaa
      841 gtagttcctc gcgcgaaatt ttttctgata ttacaggaat agaggctact attcaaccaa
      901 atagggattt caataattat gttaaagagc tcagagcttg tattaataag gaggacactt
      961 ttgacagtga gtttggatct cttattgata agctgacaaa aaattccatt actaatcaag
     1021 atatatataa tgacatactt gatataagag ttagtgggga aagtagtatt ttttcagata
     1081 aaaaattaat caattctata agaaaattga atgatgcaca agttcttgaa ttacaaaccc
     1141 ttaagccaaa tgatttagta gttatccgac ttgaactaaa tggaaaaaaa gttagtttat
     1201 cgaatgctag tgctggtcaa aagacatctg caattctttc tatgattttg gcgtatggaa
     1261 attcaccact catattggat cagcctgaag atgatttgga tagtcaatta attaataact
     1321 taatcgtaag aagtatcatt acaaaaaagg aaaacagaca aattttaatt gtaactcata
     1381 atgcaaatat cccagttaat ggtgattcag aatggttgat tagcatggga gattcaaaag
     1441 aaatatcggc tgatcagtgc gggtcggtag atgaacccaa tatcaagcca agaatatgta
     1501 ctgtgatgga aggtggcgag gatgctttta caaatagagc gaaacgctat ggttttaaac
     1561 aaattgtaga ataacactag agggtgagac atattattat gtccgccctt tttaggagga
     1621 aaaatgaaaa aaataaaaag ctataccagt atctgggcag ttgaaaaagt catttacgca
     1681 atcaatgatt ttcagttgcc ttttcctgtg acttttaacc aaatggcttg gttcgtttta
     1741 tctttactgt ttgtcatcgt atttgcccat gtgcctccac tatctatgat agaggaggtc
     1801 tttttaaaat acttaggcat accggtggct gtcacttggt ttatgtcgca aaagacgttt
     1861 gatggcaaga aaccactggg atttttacga tcctttatca gctatcactt gcgatttaag
     1921 gtgacttttg caggaaagaa agtcaaagaa cagaagaaac gatgggatga acccagtact
     1981 cttgtaagga gtgtgaacta tgtatccgat taagtatatt gaaaataatc tcgtctttaa
     2041 ccaagaaggc gagtgttttg cctactatga attagtgcct tacaattact cctttttgtc
     2101 acccgaacag aaatttcaag tgcatgataa ctttcgccaa ctaattgcgc aaaatcgtga
     2161 aggcaagatc catgcgttac aaattgccac agaaagtagt attcgcgcca cacaagaacg
     2221 atcaaaaaaa gaaattaccg gtagactcaa agaagtagcg aaacaacgaa ttgatataca
     2281 gacagatgca ttagtatcca tgattggcga tagtcagatt gattatcgct tttttattgg
     2341 ctttaaacta attgcgacag atgaagaagt caatctaaag agtctcaaga aatctttctt
     2401 ttctgggctt caagaatttg tctatggagt gaatcatcat ttgatgggcg attttgtttc
     2461 cttatccaat gaagaaattc gctgctattc taagcttgaa aaattaatgg aaagcaaatt
     2521 ggctcgaaga tttaaagtca ggcgagtcac accgagtgac ctgacgtatt tgattgaaca
     2581 tatttatggc gaaaaaggga taccgtttga agaatacgaa tttcagctac caaagaaaaa
     2641 gctaaaatca gaaacactgg tgaaacgtta tgacttatta cgtccaaatc gttgtttaat
     2701 tgaagaaaaa ccaaggtgct tacggatgga acatgagaat catgagtcgt acgtagcgta
     2761 tttaacaatt aatacaattg tgggggagat ggaatttcct tcctcagaac ttttctatta
     2821 ccagcaacaa caatttacat tcccaatcga cacaagtatg aatgtagaga ttgtgacgaa
     2881 taaaaaggct ttagctaccg ttcgtaataa gaaaaaagaa ctgaaagatt tagacaatca
     2941 tgcttaccaa tctgacaatg aaacgaattc caatgtctta gatgctttgg attccgtgga
     3001 tgaattggaa accaccttgg atcagtcaaa agaatctatg tacaaactga gttatgtggt
     3061 tcgagtgagt gccgaatcag tggatgaact aaagcgacgc tgtgatgaag tgctcgattt
     3121 ttatgatgat accaatgtaa aactagtccg gccctttggc gatatgatgg ggctacatga
     3181 agaattctta ccctcaagta aacgatatat gaacgactat attcagtatg ttacgtctga
     3241 ttttttggcg gggcttggtt ttggggctac tcagatgcta ggagaattag aagggattta
     3301 ctttggctac aatgtggata caggtcgtaa tgtttacctg aaaccggcat tggcttcaca
     3361 aggagtcaag ggttcagtaa ccaatgcgct ggccgccgct ttcctgggtt ctcttggtgg
     3421 aggtaaatcc tttagtaaca atctcttagt ctactatgct gttttgttcg gtggtcaagc
     3481 tgtgattgtc gatccaaaag gggagcgagg agggtggaaa gaaaccttgc cagaaattgc
     3541 ggatgaaatc agtatcctca acttgacgag taagtcagaa aatcaaggtc tgcttgatcc
     3601 ttatgtgatt atgaagaaaa agaaagactc ggaaagttta gcaattgata ttctgacttt
     3661 tttaacagga atttcaagtc gagacgggga gaagtttcct gtgttacgta aagccattcg
     3721 aacggtgact caaagtgatc aacgggggtt attacaagtt attaacgaat tgcgaaaaga
     3781 cgggtcacca atcgcggaaa atatcgccga tcatatcgag tcctttaccg attatgattt
     3841 tgctcatttg cttttctcag acggctcgat tacccaatcg attagtttag ataaacagct
     3901 aaatatcatt caagtggcgg atttggtttt accggatgca gaaacgactt ttgaagaata
     3961 taccaccatg gaattgctta gtgtttcgat gctaatcgtc atttccactt ttgccttgga
     4021 ctttatccat agtaaccgag aaatcttcaa aattgtcgat ttggatgagg cttggagttt
     4081 tcttcaagtg gctcaaggaa aaacgttatc gaataagctt gttcgtgctg gtcgggccat
     4141 gaatgctggg gtctatttcg tcactcaaaa tagcgacgat ttattagatg aacgtttaaa
     4201 aaataatatc gggttgaagt ttgcttttcg cagtaccgat attcatgaaa tcaagaaaac
     4261 cttggaattc tttggtttgg atcaggagga tcaaagcaat caaaaacgcc tgcgagattt
     4321 ggaaaacggg attacgtcca tataattgtg taaaagtgaa gcatcaaaaa agccattgat
     4381 ctcagttagg agtacaatgt tttttgcaga aaacatactc gaataggact gatatcaatg
     4441 actcaagtac attttacttt tgaaagcgaa gaaattcaag caattataaa cgaaagcggt
     4501 gcgaatgata ccgcaaaaac acttatgact atcatgttta atcagctgat ggaagaacaa
     4561 cgaaatcaat atattcaggc aaatgcctat gaacgttcag aagaacggca atcacaaaga
     4621 aatggttact acgatcgttc gtttacaact cgaattggta cgttagaact tcatgttcca
     4681 cgaaccagag acggtaagtt tagtcctact atctttgaaa gatatcagcg aagcgaaaaa
     4741 gctttgatcg ctgccatgat tgaaatggtc atttctggtg tctccactcg caaagtaact
     4801 aaaacagttg aactgctaac ggatggtgcg actgtttcta aatcattcgt ttcaaatctg
     4861 atgaaacagt tggatccatt tgtttttgag tggagaaacc ggagcctaga aggctcggaa
     4921 tatccatttt tcatgtgtga tgccctgtat atgaaagtaa gggaaaatca tcgtattgtc
     4981 tcaaaaggtg tatatattgg tatcggcatt gattctgatg gacgacgtac gattcttggt
     5041 tttgatgttc aagacggcga atcggaagat aattgggata ccgtttttca atcgttcgtt
     5101 caacgaggtt tgttcggtgt aaaactggtc atttctgatg cacacaaagg gttagttaag
     5161 gctgttcgca agaacttttt aggtgcgagc tggcaaagat gccaagccca ctttttgaga
     5221 aatatttttg ataagctgcc aaagaaagtt tcttctgatg taaaagacga gttgaagagt
     5281 atctttaaag cctctgaact tgaattgaca cgtgaaagaa aagagcactt cttggaaaaa
     5341 tatggttgtg attcaaaatt aagtgctgct tgcgacattt tagaaaacgg ctttgaagat
     5401 gctattcaaa ttttgtcttt tcctgaaaat atccgccgaa ggattcgtac aaccaatgtg
     5461 ttggaacggt tgaacgaaga gattcgccgg agagaacgag tgattcggat ttttccaaat
     5521 atcaattcca tcactcggat aattggaaca cttttgatgg aaaaagacac tgagtggctg
     5581 gcttctccac gaaaatattt agaatttaat tcgaataaca tttagcacca tgaacctgtg
     5641 cgagtctctt ctttctgagg agagacatca agagtcgctg cgctactgct cctgacctct
     5701 ctcctcagaa agaaaggcat ggcagttagg ttcacaacgc aaaacattgt gcttttgatg
     5761 aggttgaact ttta
//
LOCUS       DAYYKN010000156         5720 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000156 DAYYKN010000000
VERSION     DAYYKN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.158"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..717)
                     /locus_tag="ACHBB7_002576"
     CDS             complement(<1..717)
                     /locus_tag="ACHBB7_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9210470.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKV"
     gene            900..2024
                     /locus_tag="ACHBB7_002577"
     CDS             900..2024
                     /locus_tag="ACHBB7_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9210471.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            2049..2228
                     /locus_tag="ACHBB7_002578"
     CDS             2049..2228
                     /locus_tag="ACHBB7_002578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210472.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            2251..3168
                     /locus_tag="ACHBB7_002579"
     CDS             2251..3168
                     /locus_tag="ACHBB7_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9210473.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            3161..3994
                     /locus_tag="ACHBB7_002580"
     CDS             3161..3994
                     /locus_tag="ACHBB7_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9210474.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            4077..5195
                     /locus_tag="ACHBB7_002581"
     CDS             4077..5195
                     /locus_tag="ACHBB7_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9210475.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            5216..>5720
                     /locus_tag="ACHBB7_002582"
     CDS             5216..>5720
                     /locus_tag="ACHBB7_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM9210476.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVK"
BASE COUNT         1948 a          871 c         1121 g         1780 t
ORIGIN      
        1 cacttttcta aaatgttttt ttaagctggc tggactgata aaaaaaattt gggctaattg
       61 ttcaagcaaa aaattttctg aaagatgttc ttctaaatat cgtatgattg gatcaactaa
      121 ttcatctgaa taaagaatat gctttttttt aggaatgact ttctcatttg aaggtattgt
      181 caataaagca agttcctgtt gataatgcct tggtaaggaa gacaattgaa ttttttgcag
      241 acaataagta actaataagg aaagcaaatg aatgctcatc tgttggcata gtatgaaaaa
      301 agaattgatt tcctgatcat aggaaatagc tggcttacta tgttttagat aatcaatttt
      361 gaactgttca tttttcagaa aagtaatgat agatgttatt tcttgatcta tagaaaaaga
      421 ttgtcccgta atatgtgcta ataattgctc atcaaaataa aactggataa tgataaaagt
      481 ttgtgtttcc ttttgagtag aaaagatttg gacttcttgc tgtttatcaa tgaataaaag
      541 ggaaggatta tctagttcta tagatttatg tgcaaattca gcatataaat tagcgctttc
      601 atttattagt agtcgaaaat agggagcagc agataaatga agatgggtat ctatggaata
      661 ggttagtgtt tttttgacaa gattggagga ttggttgttt gttaacaaaa gaaccacttt
      721 cctttctctt tttatataaa aagctttcta tttataatta atgactatta taacaaaaat
      781 gaaaagccaa aacgacaagt aaaatatttt tttatataaa tatttgtgtt actaattccg
      841 ttaatataca taagtgaaag cgttttatat aagaacttga aaagaaagaa gggatagaca
      901 tgaaaaaaag atacaaattg gtgatagctt tgctaaacat taccctatta ttcagcttaa
      961 gtgcatgtgg aaaaactaac gaagaagtaa cagcaaacat cgcagatcca atgataaaaa
     1021 aagccactac attagaagta tatgtcgcaa acggcaaact gacaaactat gttgaaaatg
     1081 ctgcaaaaat ctataaccgc aaaactggag caaatattga cttaaaaatt gtgaatgtag
     1141 cttcgggaac agcaacagta caaatgatca ccccaaaatt ggtaacacac gaagatatgc
     1201 cggatattat ctccatggca gacacttcag cagcaggtgt gctggaaaaa tttcaagatg
     1261 ctttttatag tgctgatgaa tatggatttt atgacaaata tggcgatcag ttttatgatc
     1321 aaaaattgaa tattttgcgt caacaaacga gtaatgaaac gattattcct tgggctagtg
     1381 attttacccc agcactttgt tattatcaac caaaaaaatt tgctgaaata ggtgtagatt
     1441 ttgaagaaat cagttcttgg gaagaatata tccaggtggc aaaagcatta aaagaaaaaa
     1501 ctggtagttt tggaattgcc ttgccagaat ctggggacca agaattgttt atcaacatgc
     1561 tagcccagca gaatcaatct ttgcttgatg aagaaggaaa tatccgtctg aatactaaag
     1621 aagcgaaaca tgctgcaaag ttaatcaaag aaatgatcga ctcagatatt gtacatttct
     1681 acggtgcaca agatgctgag aaagcatttc aagaatctgc aatgtttgtt gctggcggct
     1741 ggtatgctac gaatatgtct ttgaattttc ctgatgcatc tgacaaatgg cgcatggctc
     1801 cactgattcc tttttcaaaa gaaaatccag gaaaaggtgc agtatcagga ggatcaagtt
     1861 tttatgtccc taaagatgca aaaaatccac aagtggcaca acaatttctt acatttatgc
     1921 tgacagatga agaatgctta gccaacgcac tggagctggg cgtggctaca tctaactcat
     1981 tggcctatca aacagaagca gctgagaaaa agtttgacta ctaaggagga caaaaatact
     2041 atgaactatt gaataaaatc aatgaaaata catcaaatac tgttttacca gccacttatt
     2101 cagatgctac gtcttatttg actaccgctg catataacta ttggcaaaca ggtgattttg
     2161 aaaattcata tctaaaagaa gcagaaaatt ttgctcataa gtacggcgta aaattaactg
     2221 gtgattaaaa aatattagga ggacttaacg atgatgcaat cttcgttacc aagcaagaca
     2281 ataaagaaca gcaagaaaaa agcaacagat acgtctcata aaaaaagtgc ttatggattg
     2341 gtcacacctt ttgttatttt ctttattgtg acgattttga ttcccatcat tatctcgttt
     2401 gcttttagtt ttatgaaaat tggctactca tttgaatttg ttggatttga caattatcta
     2461 gctatcttcc gagatccatt gtttattaaa tcttacacga atgttttgat tatcatggca
     2521 ggatcaattc caataacgat gctattagct atttactttg ctgttttatt gaattcagca
     2581 cagttaaaag gaaaaggatt ttttagaact gtctattata ttccaaccat tacatcaacg
     2641 gtggctgtag cttccgtatt catgactttt ttcaatccga ctgggttatt caatggattt
     2701 ttgaatgcgc taggaattaa cggtgtacag tggttaacaa atcctttttg gatacgaata
     2761 tcaatgatca tcacgatgat ttggcttagt gtaggatata acactatttt atttttagct
     2821 ggtcttcaag ggatttctaa agagatatac gaaagtgctg aaatagatgg agcatcgaaa
     2881 tttcggcaat tctttttatt aacgatcccc atgctaaagc caatcatttt aatggccaca
     2941 gttttagcca ctatcaatgg cttgggtaat ttcaatatcc caaatatatt ctttgggact
     3001 tccaatggac cagaaaattc agctttggtc gtaggagtca atttatataa aacaagtttt
     3061 gaaatggttg attttggaaa agcctctgct attgcctgga cgatggtgtt tgtatctgtt
     3121 atattatcgg ctattcaatt taaattcgga ggtgataaaa atgactaaac aaaagataca
     3181 aagcagaatt tttacgtacg tcattattgt gtttggtgcc ttaatatttt tgatgccata
     3241 tttatatatg attgtagctt caacgcaaag taatcaagaa attttaagtg gcggaatcaa
     3301 tttcaagttt ggggatatgc tattaaaaaa ctggcaagcc atcatggcga gatataatta
     3361 tcccttgatt atttggaatt cgattgttat tgctgtaatc agtacagttt tagctacagc
     3421 cagttctaca atggcgggtt atgcattagc aaagtatcgt tttatgggga ataaactgtt
     3481 gttcaataca gttatgctat ctaggatggt gccttttttt accacattga ttcctttatt
     3541 ttacattatg agtcgtttag gattatcgaa tacttatgca ggtgtgatca tcccttcgat
     3601 tgccagtaca acttcagtgt ttatgatgcg acaatatagc agtcaatttc ctacagcact
     3661 gatggaggct gccagaatcg atggtgccaa tgagtgggtg atttttacga aaatcgctgt
     3721 accgatttta tcacctacga ttattactag tggtttactt atctttatgg gaagttggaa
     3781 tcaatatcta tttccattag tcatgctttc agataaagat aaatatacgg ttcccttaat
     3841 tatcaaaaat atgacggttt ctagtacagg tgagccaatt aattatggcg caattatgct
     3901 aattttagca acttcagtga tcccaatggt cttgatttat gtgtgggcgc agtctaaatt
     3961 taaggagaat gacattggag ctgcaaataa gtaaaaaaaa taacgaatgt taaccatcta
     4021 gttcgttcta ggagcaactc cacattcata aaaattaaaa gaatggcggg atatccatga
     4081 atgaacagac gaaggaatgg tcaagaaaaa ttttgaatgt agcttctgaa aagatagatg
     4141 cggaattaga cagaatgggg aataaaattc catatatttc ttacaacgga aaatatacag
     4201 acgtaataaa ggacagagga atcgactggt ggaccaatgg tttttggagt ggtattttat
     4261 ggcagctctt tcacaagaca aaacgagaaa agttcaaaga ggcagcaata caacaagaac
     4321 aattattaga atctgccctg taccattttt cagacgttca tcatgatgta ggttttttat
     4381 tcttaccatc ggctattgca cattatcaac agtctcaaaa caaacatagt cgacaactag
     4441 ggttacatgc agctacggta cttgctggac gctataatat ttcagggaac tatttagtag
     4501 catggaatga ccataaacct ggttggatga ttattgatag tatgatgaat ttaccattac
     4561 tgtattgggc ttcgagggaa actggtgatc cgaggttctc taaaattgct cggcagcatg
     4621 cagatacaac agctaagtat ctagtgagga aagatggatc aaccgggcat atcgcagaat
     4681 ttgatgcaga atcagggaaa tatattcggt tacttggcgg gcaaggatac aatgagaatt
     4741 cttgttggag cagaggaaat gcatgggctt tatatggatt cgcgttaagt tatcgttaca
     4801 caaaaaatcc gtactatcta gctatagcag aaaagatagc agtaaatttt attcagaaaa
     4861 tcgaaaaaac tggttatatt cctctcattg attttgctgt aaaagattcg aatattgata
     4921 ccagtgctgg agtatgcgct gcatgcggtc ttctggaatt agcaggatat gtttcctctg
     4981 tacgatcaaa aatgtatcat gaagctgcta tcaatatact ccaaaagaca gaaaagcact
     5041 atgcaaattg gaatatggat gaagatggga tcattggagg cggagcagaa gcataccata
     5101 gagaactgac ttataatgtg ccactgatct attctgatta tttttttatt gaaggcttac
     5161 ttcgattaga acagtcggaa ttagctattt ggtagtaaaa agaggagaaa atacaatgaa
     5221 acatccagta ccactattaa ggaccagaca ggattacttt gacttattta aaaagataaa
     5281 tcaaccactc cagccgtttt atcgagcaag tggcgcacaa attgattata tacatcaagg
     5341 ggtaggatat ggaaataaaa ttgcaggaat ggaaggattc tctcgggttt tatggggagc
     5401 ggggcccgca attgaccaat tagatgaacg atggttgaaa ttaattctat caggaataaa
     5461 agctggaaca gatccttccc atcccgatta ttggggagaa atacatgaaa gagaccagcg
     5521 tatggtagaa atgcccgcaa tgttattagc gttatatcat catgaacaat tattatggaa
     5581 aaaattagat acaggagaaa aaagccagat tattcattgg ttctcgcaaa tttttgaata
     5641 tgaatgtgca gatggtaatt ggcaattttt taagataatt gtcggaacaa ttatccaaaa
     5701 atttggttat ccagttaaag
//
LOCUS       DAYYKN010000157         5660 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000157 DAYYKN010000000
VERSION     DAYYKN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.159"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..1162
                     /locus_tag="ACHBB7_002583"
     CDS             170..1162
                     /locus_tag="ACHBB7_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9210477.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1222..2643
                     /locus_tag="ACHBB7_002584"
     CDS             1222..2643
                     /locus_tag="ACHBB7_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM9210478.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2636..4135
                     /locus_tag="ACHBB7_002585"
     CDS             2636..4135
                     /locus_tag="ACHBB7_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM9210479.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4174..5568
                     /gene="uxaC"
                     /locus_tag="ACHBB7_002586"
     CDS             4174..5568
                     /gene="uxaC"
                     /locus_tag="ACHBB7_002586"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM9210480.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1858 a         1048 c         1163 g         1591 t
ORIGIN      
        1 taattaaatg agcattcccc gctttcaaga atagcataat taaaaataaa aatccaagta
       61 atttttaacg tgtgcaatct tttcatatca aagatctatt gacttatcgt agttatcgta
      121 atatgatgaa tgcgttaaca aaaagtgaaa aagtaaggaa aatgatggaa tggtaacgat
      181 caaagatatc gctaaagcag ctggcgtttc tcatacgaca gtttctcgtg ctttgaataa
      241 cagcagttta ataaaaaatg aaacaaaaca aaaaatacga caactagcta aggaaatgaa
      301 ttattcgcca aactatagtg caaaaggttt agtgaatcaa aaaaaatatc taatcggact
      361 atttttctca agtttgcata aaggaacttc atcaaatttc ctagcagatt ccattgcagg
      421 catccattca gtattagaca caaattacag tttatctgtt gaaagtatcg atgttgttga
      481 tttaacagaa atcaacctgc aacgctacga tggactgatc gttatgagtc aaagtgatga
      541 tgatcagcct ttcatcgact atctcaaaga aaaagaattt cctttcgttg ttgtcaatcg
      601 tcacatcaat gatccagcgg ttatcaacgt cactgccgat gatgcggccg gtgttatgca
      661 ggcagtagat tacggtattg gatgcggaca tacgaagatc gcttatattg gcggaaagaa
      721 aaattttcgt tcgacaaaag aacgggaaaa aggggtattg aaaagtctaa aaaaagcgag
      781 catccctgtc aatctagcct attttttaac aggagattac agcttagaaa gcggcctcca
      841 aaacatggaa tacctattat cactttctga cctgcctact ctggtttttt gcggcaacga
      901 cgacatagcg attggtgcat tgagagctgt acatttagct ggcctccgtg ttccccagaa
      961 catttctctg atcgggttcg atgattctcc agtcgtctcc tatctcaatc cgccgcttac
     1021 caccgtacgt aagcctttga aagaaatgtg tcaagaagga acaaagcttt tactagattt
     1081 gatcgaaggc aggacgatcg aacaatctaa aattgagctc tccaccttgt tgatccttcg
     1141 tgagtcgatc aattacttgt aaactacaag taattttata taaaaatgtt aacgtgtgca
     1201 taacacagaa aggaagttta tatgaaaaaa ctgacaaaag aattagtgaa aaaaaacgaa
     1261 gcacctgtaa aagttcttca gtttggtgaa ggcaatttta tgcgtggatt tattgattgg
     1321 cagattcagc agctgaataa ccaacaattg tttaaaggta atgttgcaat cattcaacct
     1381 ttagaacaag gtcttggaaa tatgatgaac cagcaagata atttatatac ggtcatttta
     1441 caaggactca gcaatggcga aatcgttgat acttccgaga ttatcaccgt agtagaacaa
     1501 atgatcaatc cttatgatga atgggatgct tatctatctc tagcagaaaa tgacgacttg
     1561 gcctatatcg tctccaatac aacagaggca ggaatccaat tcgtggagac tgataattta
     1621 acagatactc ccccacagag cttcccgggc aaactgacag cctttttata ccgacgtttc
     1681 aaattaaaca aagctggctt taccatcatt ccttgcgaac tgattgacag aaacggcgaa
     1741 cgtctaaaag aaatcatttt acgctacgca gaaaaatggg atttagaacc tgcttttgtt
     1801 gagtggttag aaaaagaaaa tgttttctgt tgtagtttag ttgatcgcat cgtcccaggt
     1861 tatcctcgcg aagatgcagg tgttttcaat caacggcata actatgaaga tcaattgatg
     1921 gtcaaagcag aaccattcat gctttgggtg atcgaagggc ctaaagaatt ggagaatcaa
     1981 ctaccattca aacaggctgg cttgaatgtc atcatcacag atgatatgac tccttatcgt
     2041 gaacgtaagg tgcatttgct taatggaccg catacagcga tggtccctct tgctttgttg
     2101 gcggaattgg aaactgtaga agaagtaatg aaagatcctt tattctccgc atatgttgat
     2161 cagttattca atctagaatt gattcctatg ctttctttac ctaaagatga attagccatt
     2221 tatgctgacc aaatcaaaga acgtttcttg aatccttttg cccatcacaa attagaagcg
     2281 atttcattaa atagtgtttc aaagttcagc acccgtctgc ttccagtgtt caagaagtat
     2341 atcgaagaac aaaatcaggt accaccgtta atcactgttt cactagctgc gctacttctt
     2401 atgtatcgag gcgatcaagt aaagccacat gatgatgaaa agacgataag cgaatttact
     2461 gatgcttggt ctgataatgg aacagctatt cctcgattat tgcaaaacgc tgcgctttgg
     2521 ggagaagatt tgtctcaaat accgaatgtg acagatacag ttcaagaaat agctgcaaag
     2581 atcgatcaaa aaggtgtccg gtcagtatta caagaaatga aaggaacgat ggtccatgct
     2641 tgaaaccctg caaccaacaa tgaaattaaa tcgacgagac agtgtggtcg tcgcattaac
     2701 ccctattcca aaacaaacac aactgacgat tgatcagcaa acgatcacca ctcttgaaga
     2761 tatcccgcaa ggtcataaga ttgcattggg tgatttgaaa gctggagaca acgtgatcaa
     2821 atatggctat ccaatcggtc atgtgacgac agacgtaact gccggtcaat ggctgcacac
     2881 tcataatgta aaaaccaatc tttctggtga attggattat cgatatgaaa aagatgtcca
     2941 tccaagtcat tacctttttg agaaccgaac atttcaaggt tacttgcgaa aaaatggaaa
     3001 agtagggatt cgtaatgatt tgtttttagt acctaccgtt gggtgtgtga atggcattgc
     3061 tgaactgatc gtcaaacagt tcaaggaaaa acatccagat ctgggacagt ttgaccatat
     3121 tacgatttta aagcatcctt atggctgttc gcagcttgga aaagatcatc aaaatactcg
     3181 agaaatttta gcggacgcag taaatcatcc caatgccgga ggagttttag tatttggatt
     3241 aggttgtgag aacaatacag ttcctgaatt caaaaaaatt ttaggtgaat atgatgaaga
     3301 acgagtgaaa tttctagtgg ctcaagatgt ttacgatgag atcgaacaag gtgtggctct
     3361 attagaagag cttttaacag cggcggaaaa tgaccagcgc atatcagtcc cgctctctaa
     3421 gttgaatgtc ggtttgaaat gcggcggttc tgatggtcta tcgggaataa ctgctaatcc
     3481 tttacttggt gcattttctg actacttgat tgctcaagga ggcagcacga tcttaacaga
     3541 agtgccagaa atgtttggcg cagaacaagt attgatggca cgagcggaaa atgaagaagt
     3601 ttttgactca atcgttgact tgatcaatga cttcaaacag tatttcttgt cttacgggga
     3661 accagtttac gaaaacccat ctcctggtaa taaagcaggt gggatcacta cattagaaga
     3721 taagtcttta ggctgcacgc aaaaatcagg aagctctcct gtcgttgatg tgctacaata
     3781 tggcgaaaaa atcagaaaac cagggcttag tttattgcaa gctcccggga atgatcttgt
     3841 agctgcctct gctcttgctt catccgattg ccagttggtc ttgtttacca caggaagagg
     3901 gactccgttt ggctcatatg tcccaacggt caaggtatct acgaatacaa ctctttttga
     3961 acgcaaaggt cattggatgg attttaatgc aggcgtctta ttagaacaac caatggaagt
     4021 aatcttagaa tcgtttgttc aaaaaatcat cgctgtagct agtggagaag aaacgaaaaa
     4081 tgaacaaaat gacgttcgag aaattgccat ttttaaaaac ggagtaacac tttgattttc
     4141 ataaaacagt ataaaaagaa agaaggaaga tttatgtttt tatctgatga ttttttatta
     4201 aaagacgatt gggcgaaaaa gctttaccat tcctacgcaa aaaacatgcc cattattgat
     4261 tatcactgtc acttagatcc aaaagaaatt tacgaaaaca acaatttcag caatctgaca
     4321 gaagcttggt taggaggcga tcattacaaa tggcgcttga tgcgagcttg cggcgtgcct
     4381 gaagaaaaaa tcacaggaaa tgcttctgat tttgaaaaat ttttagcttg gtgtcaaacc
     4441 gtaccaaaaa ttatcggaaa tccgctttac agttggtcac atcttgaatt gaagcgtttc
     4501 ttcggtattg acttgttgat aaatgaagaa aatgcagaga aaatttggac acaagcaaat
     4561 gaaattttgg cagctcctga ttttcgccgt cgggaaatcg caaaaaaatc gaatgtagaa
     4621 gtgatttgta caacagatga tcccgcagac gacttgcact atcatcagtt gcttgctcat
     4681 gaagaaccag atttgaaggt tctaccttca ttccgtccag ataaagcact caatattgaa
     4741 aaagaaggat ttaatgcatg ggtcacacta ctggaagaag cagctgacca aacgatcacc
     4801 acctatcaag aactgataga agtcttaggg cagcggatag aatacttcca tcaacatgga
     4861 tgtcgtgttt ccgaccacgc tttagacatc ttacgctata aagaagcgga tgaaacagta
     4921 ttagaagaga tttttcaaaa agcacgagct aacaaactat tatcttcaga tgaaatcgat
     4981 gcttatctta cagaaacact gatacatttg acccacttct atcattctca taattggacg
     5041 atgcagctgc acatccatgc ttatcgtaac tgtaatacac aaatgttgga aaaattaggg
     5101 cccgataccg gctatgatgg aatgaatgat ctttctttga ctattcctct gcaaaaatta
     5161 ttgaaccgtg cagaagaaac ggatcagttg ccaaagacga tcctttattc attgaatccg
     5221 aatgactatc ctgcgatttt agctttgatg ggctgtttcc aaaaagaaca gaatggaaaa
     5281 ctgcagttag gttctggatg gtggtacaac gatactcgag ctggtatgcg cgatcaattg
     5341 acacaatttg ctgatggtag tgctttgggg aattttgtcg gcatgctgac agattcaaga
     5401 agtttcctgt cctacactag acatgaatat ttccgtcgtg ttctttgtga atttttaggc
     5461 gaaatggtca tgcgaggaga agcacctgaa gatgaacaac ttttaggaag tctagttcaa
     5521 gcgattagtt atacaaatgc taaaaattac tttggctttt ttgcctaatt gattaatagt
     5581 aacagtaacg aatcaaaaac aggatagtga aataacggaa tccgtttatc cacgttttag
     5641 agaagaagac ggaccaaatt
//
LOCUS       DAYYKN010000158         5530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000158 DAYYKN010000000
VERSION     DAYYKN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.160"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHBB7_002587"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHBB7_002587"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFM9210481.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHBB7_002588"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHBB7_002588"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFM9210482.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACHBB7_002589"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACHBB7_002589"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFM9210483.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ACHBB7_002590"
     CDS             complement(4028..5044)
                     /locus_tag="ACHBB7_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9210484.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5298)
                     /locus_tag="ACHBB7_002591"
     CDS             complement(5146..5298)
                     /locus_tag="ACHBB7_002591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210485.1"
                     /translation="MDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGIEF
                     KISDVQ"
     gene            complement(5315..>5530)
                     /locus_tag="ACHBB7_002592"
     CDS             complement(5315..>5530)
                     /locus_tag="ACHBB7_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFM9210486.1"
                     /translation="YEFVGMSVRKNGRLVIKYAKDNHAKRFMREENRKIDQKEFRQQY
                     EEEKEKTFKAIRWDWERQREIYMSKLW"
BASE COUNT         1452 a         1370 c          970 g         1738 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata
//
LOCUS       DAYYKN010000159         5528 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000159 DAYYKN010000000
VERSION     DAYYKN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.161"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..293)
                     /locus_tag="ACHBB7_002593"
     CDS             complement(<1..293)
                     /locus_tag="ACHBB7_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM9210487.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLL"
     gene            386..735
                     /locus_tag="ACHBB7_002594"
                     /pseudo
     CDS             386..735
                     /locus_tag="ACHBB7_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1007..1297)
                     /locus_tag="ACHBB7_002595"
     CDS             complement(1007..1297)
                     /locus_tag="ACHBB7_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210488.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1699..1935
                     /locus_tag="ACHBB7_002596"
     CDS             1699..1935
                     /locus_tag="ACHBB7_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210489.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1942..3894
                     /locus_tag="ACHBB7_002597"
     CDS             1942..3894
                     /locus_tag="ACHBB7_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM9210490.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3966..4463)
                     /gene="dfrG"
                     /locus_tag="ACHBB7_002598"
     CDS             complement(3966..4463)
                     /gene="dfrG"
                     /locus_tag="ACHBB7_002598"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM9210491.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4790..5482)
                     /locus_tag="ACHBB7_002599"
     CDS             complement(4790..5482)
                     /locus_tag="ACHBB7_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9210492.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1797 a          963 c          898 g         1870 t
ORIGIN      
        1 caaagtaata ctacaattcc taacataatc gtagaattta ataatttcca tagcataaag
       61 tgtttagaaa agaacattaa gaatagttct ataataattc ttgaactcca tgtcttataa
      121 tgttctatcg aagcacccaa atagccaagt tcatctaatc tacttgcata aactacgtca
      181 tccgctctca tagtcggcat atacatatta aagagtaaaa agatacccca aatccctatt
      241 agtaacaaga tcagttcttt gttattcgtt atttttctat tatgtttgtt catttttata
      301 ctccttctta tatttcctaa tagagtataa catagcttta agcattactt aaagataact
      361 taaaagttat cttttaaata cttaattgtt aaatacctta tttgatgcaa aagaaatcta
      421 cgatagtcaa caaaaaaatt ttcattatat ccagaaaaat ttttcttagt taaggaccta
      481 tggattgcag taatgcagaa ttcgtcgaat aaactgccaa agacatacaa tcatattgct
      541 tttaaaattg acgaacaaga tatagattcg tttgtctcaa aaatccaaat gttaggttta
      601 actgtcgaac caggacgttc tcgtgtgaaa ggcgaggctt cttcaattta cttttacgac
      661 tatgataatc acctcttcga gttacatacc ggtactctcc aggaacgttt aaaatcctac
      721 aattccacta cctgagacta gtaatgacta gtctcttttt atggcctctg atccccccat
      781 tgagcgaata ctaatagggc ttcgaattga tctgttcaat ctttaagcaa tatccctcaa
      841 atgagtttgg aaagtttagt gattcatcac aaatacttaa atacatgtag ttatatatag
      901 attgaggttc ttgtgagcgt atgctcgtgt gggttcaaat cccatcgtct gcatccatct
      961 ttccaaaaca agcggctagg acatttttgt ctcagccgct tgttttctag tttattcttt
     1021 catcttgtcg gacctttttt cttgggtata gacttttctc atattctacc acaattttgg
     1081 gtttccctaa cacatgatag gatctcacaa caaattgctt tctattatta taataacctg
     1141 taacgactaa cttcatccct tcgtctactt cagctaggaa gttcaaacta tgggaagcaa
     1201 tcaaacaatt gaccccattt aaactgaaac ggatcaacgg tctggcacta acttgcaaag
     1261 tacggatctt atctactgtt ccatattttt tctccataac cttcacctca ataccattat
     1321 acgaacatac attccctttg taaagaagaa actctgtttt atctttttga ctttttgtct
     1381 attacctttc ttaaaatcat attggcactt cctgcgaaga tgaaggacat gtactaaaaa
     1441 aaagaggcag cagtatagat ttaagtagtt cagttttgga gtacaaaaag tgaaaatgta
     1501 aacctaaatg agtatcaaat tgcaactgaa tttgatactc gttttttatt gttaaatcaa
     1561 cgtttgttgt taatttacac cacaaaatgt aagttaaaat gtatataaaa atgcgaacgt
     1621 gaaataatta ttgtaggaat gcaaattggt gtgttaaaat ggttacaatt tactaagcaa
     1681 aaaggggtgc attttcctat gcaatttctt gagccaaaaa ataagaacgc taagtctgtg
     1741 gattgggaaa tatcagagca agtaagggtg atagttaagc aatacgctga atatgcagag
     1801 cgtactgaaa gcgaagcagt ggatgaattc ttattgaaca ttttagacga taagaagttt
     1861 attgaatgga ttgccaacaa gcgaagtaat aagcggattg ttgaaaaaat gggtatcaaa
     1921 gatagagtgg gttgatagac aatgaccaag cttaaaagat tagccacgaa ggaagatgaa
     1981 attgtagatg ttaaaattcc gatttcaaac gaagaattaa aagatagggc taaacaatat
     2041 gatttattaa ctccaaaacg atttgcaaca aggtataaca aaatgttgtt cttgccaacg
     2101 tcttttaaat ggaacggttc tgaatatccc attcaatata attactgcat caatccgttt
     2161 tgttgtaatt ttgggaaaga acagcacaaa ttcaaagatg taaaagggaa accaagtcga
     2221 tataaaatga ctggtagttc caaagataaa ggtcataaag gtatgtactg caatgacaat
     2281 cctattggca gaggtgtatc gcaaaactgt acagtaaccc ctttatctaa ttggtctgtt
     2341 gttgaagaaa ttaaacgcct tatagaaatc aatagcatcc aagatgttga gccagattat
     2401 caatttcata aagaaggctg ctcggaagaa gaatcaaccc cattcaatga gccgaagcaa
     2461 ttttacaaaa gggggaaaag taggggcaaa tctcaacgct atcaatgtaa ggcgtgtaag
     2521 aagttcacta atgttctacc aaaaagagaa gaaaccacta cttatcacca acaaaagaac
     2581 accattcttc caatgttcgc taagatggtt gttggaaggg tttcagtcag tcgtacttgt
     2641 gatattttag gaattggagt tggaacctac tatcataagc tggagtggct gtataggcgt
     2701 tgtttggagt ttttagaacg atatgagaca caaccccttc aaacaaagaa atttaacgag
     2761 atgtggctta atacggacaa gatgcactac tacctaaata acgtccgtaa gaaaggacaa
     2821 ggttcaaaga aatatacggg gtttgaagac cttaatatgc aaacgtatat agttgtatct
     2881 gctgaggtct tatcaagata tgtgttccgt tctgatgttg cctatgattg gaatatttca
     2941 atggatgaat taaatgaaga tacaagaaaa ttcaaagaag accatctcaa tacttttagt
     3001 cgtaaaaatg ataggttaga ttggtcttat tatccacaag aaccatccgc taacgatagc
     3061 gaaaatagga atgcttatct tcacgaatta gggaaaatta ctaataggag tagatttgtc
     3121 gatggtttaa atgttgatgc tccttatact acaactgctc actattggtt aattaaacaa
     3181 atggttaatg ccgacgaatg gcgaatgatt agtgatgatg acttctctat tagaaacgcc
     3241 ttttatagag tattcacgaa agaattaagg ttgagcgatg cacatcattt tatttgccaa
     3301 gttaacaaga cgaaaagcag aaaacaatgc ttaaaagaat tcggacaagc aaaagcagaa
     3361 ctgctcgatt ggggagatat cagaggattc aaaacaaagt ttctacggac tttggcatct
     3421 cattatctaa ctgaattact aacaagtcat caattccacg aagaggctat tagtaaagat
     3481 ggagaaagat atcgcaaata tgccgataac cctatcaaac atccattagc cactaaggat
     3541 aaaggatttt attcagttga ttgtagaacg gatttatctg ctctggaacc aaacgaaatc
     3601 gcaaagatgc tattaaatgt taatgaccat tcgaccaata gctttattca acagataaga
     3661 cgttatatat catctcttga aagaccatta acaacagctc gtggtgataa gaagagctat
     3721 atttacgcta acttcaatcc gaagtatgct caatttgcga ttacaattct aagaacttac
     3781 tacaactttt gtcgcccttt taaatcagcg gataaaaagg ttttgacacc tgctcaaaga
     3841 ttagggatta cagataaaca atttgattgg aaagatataa tttatttcaa atgacaaaaa
     3901 gagcttggag taatcccaag ctcttgtgct ttatctttac tgttcttctt caattaacgc
     3961 acccgttaac tcaataagtt ttttctttca tatacatgaa aatagtagtt atacggattt
     4021 ttatcattct ttatcccttt ttgggcaaat acctcattcc attcctcata attcacttct
     4081 ggaaaaaaag tatctccttc gaattcatga tgtatttttg tgatgtacat tttctcaaca
     4141 taagggaaaa acaaattata aatctgttct cctccgaaaa taaaaatttc ttcttcgttt
     4201 ttacataact caaaaacatc ttctattgaa tgaacaattt cacaaccatt aaaggtaaac
     4261 cccttatctc tcgtcagaat aatatttctt ctgtcaggta aggctcttcc gattgattca
     4321 aggttcttcc tacctaatat tatcggatgt ccctttgtag tatttttaac atattcccag
     4381 tccttgggaa tcctccaagg aatgtcattc tctttgccaa tcactctatt cttatccatc
     4441 gcagcaatca aagaaacttt catttataac acagcctttt taactaattt accatctaat
     4501 ttcttatttc tatcaaaaaa ccttttctca ttatcctgcc cgttagttga ataagcaatt
     4561 ttttctataa gttatattat cataatacca caaaatcaca aattttcatt caattatcat
     4621 tttcaattgc attttataaa tcattttgta tgtaatttgg tatgtaagtt aaaatgaaaa
     4681 aagtagtcca ttttgaagta cattttgtca ttttgtactc caaaactgaa ctacttagta
     4741 tagattactg ttgcttcttt actaatttag gtttacttgt taagaaagat tatttacgca
     4801 aatgttcttc aacaatttgc tgagatttat cagctgtatc ttccacttca tcttttgctt
     4861 tttctaactt ttgaatatat tgtttcaagc ttgagttttc agcagttgct tcgtctaatt
     4921 tcttttgtgt atctgttaac tgagtttcta catttgtttt ttcttgtgtc agtgttgcga
     4981 tttgttgatt caaaccattt acagtatttt gtaattctga aagttttgct tgtgcttctt
     5041 gtagtcttgc tgtccaatct gcattcagat gattgatttc atcatttttt tggttgatct
     5101 catctagttt gacattgatt tcattttgtt tgctttgtag ttgtgcatcc ttgctgacta
     5161 attgactttc taagttttgt ttttctgctg ccgattgatt tttttggttt tctaattcat
     5221 ttacttttgt ttggtattct tggacatctg cctttgaatc ttgcaatgtc tgttcttttt
     5281 gtgctagtcg agcggtaaga tctgtaatcg cttgctcttt atctgtcaat gtatgatcaa
     5341 gctgtgtcag attctcattg attgtatcaa tatctgtctc tccaccccac aaaataattt
     5401 catttgcgta ccgcgtgcct gtgtagccaa ttagacatag aaatgcaacg acaatcccac
     5461 cagtaataaa tttctttttc atttgcatag ctccttaatc caatactttt acatgctaaa
     5521 agtaagtg
//
LOCUS       DAYYKN010000160         5458 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000160 DAYYKN010000000
VERSION     DAYYKN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.162"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..1530)
                     /locus_tag="ACHBB7_002600"
     CDS             complement(25..1530)
                     /locus_tag="ACHBB7_002600"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM9210493.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1622..2212)
                     /locus_tag="ACHBB7_002601"
     CDS             complement(1622..2212)
                     /locus_tag="ACHBB7_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9210494.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2391..4037
                     /locus_tag="ACHBB7_002602"
     CDS             2391..4037
                     /locus_tag="ACHBB7_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFM9210495.1"
                     /translation="MDNQRKPSLDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4086..4805
                     /locus_tag="ACHBB7_002603"
     CDS             4086..4805
                     /locus_tag="ACHBB7_002603"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9210496.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5284..>5458
                     /locus_tag="ACHBB7_002604"
     CDS             5284..>5458
                     /locus_tag="ACHBB7_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM9210497.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLA"
BASE COUNT         1648 a          994 c         1102 g         1714 t
ORIGIN      
        1 gaaaccacta gtatataaat accattattc ttcaatcaac cgcttcacga ttgcataatc
       61 cccttcataa ggttcatcta caccattgat tttttgatag ggatccacgc cttttcccgc
      121 aataacgact gaatcttgcg gagcgctttc tgctaaagcg cgtcgaatcg cttcaggacg
      181 atccatttca taatgaacga tcaaatctgg attagtaatt gcttcattga tttcttccgc
      241 gatttttttc ggatcttcaa atgctgggtc atctgccgtc aaaaaggcga tgtcagctgt
      301 ttctgatagc acttttccaa aatcatgtcg acgggagatt gctttgtttc ctgggctgcc
      361 taatacaaca attacacggc catctggatg ttcatttttg gcaaatttca gcaatgtttt
      421 caagcttaga taattatgtg catagtccac atagacatgc gctccattgg attggatcaa
      481 ttgatccatt cggcctggta cacgagcttc ttttaatccg tcttgtgcat cttgactgga
      541 ggcacctgtt agtgctgcgg caatgatcgc actggctgca ttttcttggt tgaatcctcc
      601 agccagcaaa atatcataag cagtatcgca gatagctaag cgatcttcat gagaagataa
      661 agtaaaaccg tgagtacctt tatctgaacg gatcacttga taattgcttt cttccgaacg
      721 accataagtg atcgttggaa tctggaatag atcggctgtt tccctaagta aatcaaaata
      781 gtcactttca tgatttaaaa caactgtttt tgaattcaag atcaattgtc gcttgcaata
      841 gaaatagtca tcaaatgtcg gatgttcgat cggactgata tggtcaggcg agatattcaa
      901 gaagattccg acatctaaag tcaagccgta tacgcgttgt gttttgtaag cttgtgaaga
      961 aacttccatc actagatgag tcataccatt ttcaactgct tctgacatca tacgatataa
     1021 atctaatgac tcaggcgttg tcaaatggga tttgaaatat gttttgccat ctaacgttgt
     1081 attcatagta gaaagcaatg ctgttttttt atttgttgtg tgatccaaga ttgcttttgt
     1141 aaagtacgca gcagttgttt taccttttgt tccagtgaat ccgatcactt tcaatttatt
     1201 ttgcggatag tcgtaaaaag ccatagcaag aagcgccatt gctttacgga tatccgttac
     1261 gatgattcct actgctgaat tttcgtatgg ttgttctgat acataatatt gaataccctg
     1321 ttcgattgct gaatccaaat attcttcttt gaaattcatt cctttacaga aaaacaaagt
     1381 agaagcatca gcttttcttg aatcataact taactgaaga aattcagctt tttctggtac
     1441 atcaagatgc cagcctcttg ttgaaacaaa ttcttttaat agattttctt ttaatagtaa
     1501 atgacggatg tcttctaaag agaagctcat tgattgatcc ctcgctttat tcaatttctt
     1561 ggtttattat agcacactct ttttaatata tcgtttgttt ccgctatgac aaaatccagc
     1621 tttattcaaa ttttcttgac acaggggaca agcggatgag tattttcata aacaatgaac
     1681 tgactgtaat agataggaga acgaatagtg tctccaacaa tccaaaaaca taataattca
     1741 aaaagacgat aagactattg gtaagtaaaa gtccttgcgg cattggaagc cgaaaaaatt
     1801 tgtggaaaat aatcggcggt atcgttatac cgccatttga ggccccgact gtgtacaatg
     1861 tgtaaacccc aagactgaac aaaagacttc ctgaaatcag tgaaaagaaa tgtgaggaga
     1921 tcatttccac ttttggaaca atctctaaaa caacaggaaa aagcagactt cccgctacag
     1981 tcttaaaaaa tactgatttt cctaatagga cagcggctag aagcagcatc ataagattga
     2041 tgctccaaac agtaactgag cgttggatag aaaaggccgt ttcaactaaa tgtccgatgg
     2101 cgcccactcc ggcggaagct atatcgtgag ggagcaagaa ccaattcaat ccaactgtga
     2161 taaagaaaag acctaaaaat ataacgggta gtttttttat ttcttgtgtc attgatatct
     2221 cctataaatg ttagccataa aaggctgtat atttctttac tttaccaaaa aaacgaaacc
     2281 gaattcaaaa aaacaattgt tcttataaaa gttatataaa atcgattgcc agtttctgtt
     2341 ttaccaatta aaatgatagt aatgttttgt agaaagttgg aatctgaccg atggataatc
     2401 aaagaaaacc atcattagat caattaacac cgcaagaaaa aatggcacga ggctctgctt
     2461 ggatgaccgc cagtaatatc atttcaagat tgctaggcgc tgtctatatc attccctggt
     2521 atgcttggat gggcgaaaat gctaaggctg ccaatggatt attcaatatg ggatacaata
     2581 tatatgcatt gtttctactg atctctacag caggaatacc tgctgcgatt gcaaaacaaa
     2641 ctgcacgcta taattcattg aatgaatatg gcacttcacg acgattattt atccgtgcat
     2701 tacagatgat gggcgggtta ggcctgttgt ttgctttgtt catgtacatc gcctctcctt
     2761 ggctagctca tgcttctggt ggcggagaag aactgattcc aacgatgcgt tctttgagta
     2821 tcgctgtatt ggtttttcct tgtatgagtg tgatacgagg gtatttgcaa ggcaatcaag
     2881 aaatgatgcc ttatgcgctg tcgcagattg tagaacaggt ggcacgtatt ttttatatgc
     2941 tattggctac attcatcatc atgaaagtca tggatggtga ctatgtgacg gcggtcacgc
     3001 aatcaacttt cgcggcattt atcggtatgc tagcaagtat tcttgtcctt ttgtattttt
     3061 tgaagaaaca tcaagcgtat acatcggcgt ttattcacta cagcgaaaac aaaataaata
     3121 ttgcaacgaa ggaattgttg ttagataccg ttaaagaagc gattccgttt attattgtgg
     3181 gttcaggggt aacgatcttc aaattagtcg atcagttcac ttttatgaag atcatgtctg
     3241 catccaccga gtattccaat gcacagttac tggatctttt ttcgattttt agtgccaatc
     3301 cagataagtt gacgatggta gttattgcat tggcaacatc gatcgcgtct actgggttac
     3361 cgttgattac agaagctgtt acgttgaaag atcgagtagg tctggcaaaa ttgacaagcg
     3421 gaaatttaca gttgttttca ttttttatgt ttccggcgac atttggtgtt atgttacttg
     3481 cctatccgct aaatacatta ttttatacac cggattctct aggaagtaat gtactgatcc
     3541 aagcaagttt tgtaggactc ttcctaggac tatatatgct agtttccaac atgctgcaag
     3601 gaatgtttga aaacaaggcg gcaattaatt atttggttat tgggtttcta gtgaaactcc
     3661 ttcttcaata cccagccatt cgtttatttg aagtatacgg ccctttattg gcgacaatga
     3721 tcggatttgc tgtgtcttgt accttgatct taaagagaat ccatgaagta gcccgcttta
     3781 aaccaagttt tgtctggcgc aggacattgc tgatctttat tcttaccttg attatgcttt
     3841 tggcttcgtg gatcacaaag atgatttttg gtgctttttt aaacgaagat agcaaattcc
     3901 agtctctatt gttgattctt gtagtagcag gtataggggc tttagtgtat ggttatttag
     3961 ctttgaaaat ccgcttggca gaaagactac taggaccagg aatagcgcgt ttacgacata
     4021 aattaaaaat aaaataagtg aatcagaaga gctagattga tgctcttttg aaatggggga
     4081 aacgcatgcg tttagataag tttttagcag atatgggact tggcagccgc aaagaagtca
     4141 aaggattaat caaaaaagga acgatcgaag tcaatggaca agtggtgaaa agcgataaag
     4201 cgcaagtaaa tgaattcgaa gatgcggtta cttatttagg agaaatcatt acgtatcaaa
     4261 aagattttta ctacattttg cataaacctg caggagtcat ttctgctact caggataatc
     4321 atgatcaaac cgtaatcgat ttattggaag accaggatta tcgggaagat ctttttccag
     4381 tcggccgatt agataaagac acagagggcc tcttgatttt aacgaatgac ggggtgtttg
     4441 cccatcagtt actatcacct aaaaaacatg tggagaaaga atatctagct gaggttagcg
     4501 gtataatgac agaagaagat atccgtttat ttgcagaagg actgattatt gatggcgatg
     4561 agcagacatt gccggcaaaa ctccaaattg atcaagtaga tcaagagaca gaaacttcaa
     4621 ttatccgtct gattttgcat gaaggaaagt ttcaccaagt taagcgaatg gtcaaagcag
     4681 taggaaaaga agtcttatat ttaaaaagaa tccgtatggg aaaattttgg ctgcctaaag
     4741 atttgaataa agggcaatat cgacccatga ccaaagaaga gctagaacaa gtgagagaaa
     4801 aataattgaa tggatagcta aaagaataag cagagtagtt gtagcgccat ctgaaaagca
     4861 gacttgtccg tctgactttc ggatggcttt tttgtgaaat ttcggcttat ttccgtagaa
     4921 gctacttctg gaacaccgtt tgttcggaat aagggactgt gacaagactt ttgttacagt
     4981 ccccattgtt ccttgtgact cagagttaga tagtaaggac acgatctccc taatactcag
     5041 gccaaaacgc tgaattcaca acctctgata aacggtgttc aatcagctga ccctcggtat
     5101 tagctcaaaa aacggcaaaa tatgaggaac tattttcccg tttttttctc taatactcag
     5161 gccaaaacgc tgatttcaca acctctccta tactttgttt cattttccct atactttgtt
     5221 tatttattct cattattgaa agcgctataa ttacgttgcg aatgaatcga aaggggtttt
     5281 tagatgaaaa aacgaaaggg gttcaatcat ttttttatga atctatggcg ttataaagca
     5341 cttgtcctga tggctgtacc gggtatgctt tggatgattt ttttctttta catacctgta
     5401 atggcgaatg tcgtagcatt caaaaatttt catatttcag cagatgggtt cttggcta
//
LOCUS       DAYYKN010000161         5367 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000161 DAYYKN010000000
VERSION     DAYYKN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.163"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..2374
                     /locus_tag="ACHBB7_002605"
     CDS             47..2374
                     /locus_tag="ACHBB7_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFM9210498.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2371..3285
                     /locus_tag="ACHBB7_002606"
                     /pseudo
     CDS             2371..3285
                     /locus_tag="ACHBB7_002606"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3358..3873
                     /locus_tag="ACHBB7_002607"
     CDS             3358..3873
                     /locus_tag="ACHBB7_002607"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM9210499.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            4001..5299
                     /locus_tag="ACHBB7_002608"
     CDS             4001..5299
                     /locus_tag="ACHBB7_002608"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9210500.1"
                     /translation="MHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYP
                     SKDTAVPADATPFASVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLS
                     LPFYNPNSSDPDKPLDTIHLYAKNVIKESNIKIQKAGNFSGVDKLAGAQFQIKNSENQ
                     FVTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVETKAPDGYQLTNPQ
                     KETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTITVENVQNYGDFHF
                     IKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWSDEVTGSDWEEVKL
                     ISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDDDPTTSDETTIDNQ
                     PKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
BASE COUNT         1903 a         1028 c         1063 g         1373 t
ORIGIN      
        1 aataacgtgt aaaggattat ttgatgattt tttattgaga ggagggatga aaatgattaa
       61 gaaaactcaa aataaaatcc aacgtatatg tcaaaaacat tttgtttcta gactcaaagc
      121 tgtatctaaa agaaaatact tacatcccat tatgatttcg atcatcttga tttttacttt
      181 cttagggagc ttatcaagta tttatgtaaa cgtttataca gaaacgtcaa attttgctaa
      241 ccaaacgaca gaggagacaa atagtccacc ctattctgaa atacagtcca tagaaagcac
      301 caatcagtct agcaacaacg agacaacctc aaccacatcc tcgactcaac tgattgatcc
      361 gacagaaaaa gaaatcacag cagcaaaagc agcaggggag aaagcttatt cagaaacagg
      421 tagaagccag acatttagta ctgttgcggc cactgcgaat ttcaacggac ataccatacc
      481 agatggatat acagtgatat atgccaaaga tggagtcatt gttgcggtta acggatcgat
      541 gagtgcggat aaaactgcgt tacaaggcgt aaccaaagca atcgctttta ttgataaccg
      601 tccagatatc cataatctgt ttattggctc cacagtaaat ggtactacta tcacattccc
      661 aaaaagtatc tatatggggg tggctaccgc tggaaatacg atcgattcag ttacacccaa
      721 tgcggataaa agttattctt ttacaccctc aacatgggga gggcttcctg ccggaggagg
      781 tatgagtact ttttcaatcg gtatacagtt agcaaatgat attccattca cggggtactc
      841 gatcaaaaat gggcagatcg ttgatggtaa gggaaatgtt tcaaattttc caacctatca
      901 agcagcaacg gttcaggtca atgcgaatcg gacaggaact ttttcttata cagtcaatgg
      961 tggagcagca aaaatcggaa cgtacgggac tacattatct ggaatccatg tgggtgacaa
     1021 agtggttatc actccaacag cagtaacggg ctattctttt gcacaaacaa acggttcttt
     1081 cacagcccta gaaagtaacg tcgatacggt gacctataca ggcaaacaaa caccaatcac
     1141 tattaagtat caagactatt ttggacagat gattgctcct tctaaaataa tgaatcttac
     1201 ttacggaagt gctgcacaag acctgacgac taatacgcct actatcaacg gatatacctt
     1261 tacagctgtt tcagcgacag aaacaaagaa ccaatcagca atgagtgttt tagctagttt
     1321 agatacaaat ggaaatgtaa tcgtaaagga cgcaaatgga aaacagattt ctgaagtcat
     1381 cctatattat aagacaaatg caacagtatc aatcaatgca aatggcagta agtactatga
     1441 tggtctttct gttgtaccga tcgttaaata tgcctttaat gataaaacag aatcaacatc
     1501 ccttttaaac aatgatctca gtattgatca aatcacatgg aatccaactg atttcaaagc
     1561 gatgaatgaa aagggagttg aagtaactgc gcctactgaa attggaacct atacgtcttg
     1621 gcaattgaca aaaagcggac tagctaaact agctgccaga acaaactatt tatttactat
     1681 cgtgcagaca tctgatgtct atacgattaa gcaaatcagc ggtgcggtag agctcagtgg
     1741 cagtaagaca tatgatggaa aagcaggtgt cccgtcaatc catgtcaagc tagcagaggg
     1801 tgtcactagt tcgcttactc cagtacaagt ggcacttagc agtatggatt acacagcaga
     1861 tgcacaatca gcaaaaaata tggttaatgc tggcagctat acaattaaac taacgaatag
     1921 cggaatagac aaagtcaaag cagcaaatcc caagttgtca tttactgata tagccaacac
     1981 atcaggaact tataccatct ataaagctaa cgcagtgatt actgtcgatg acgctgcctt
     2041 taattatgat gcccaaagtc actcgatttc ggttggaaat ctccacgtta ccggtgttgt
     2101 cttaggcgaa tcattggatt ataccctaac agttaacagc cggacagata ttggatcaca
     2161 aatagtaacg attagtttag gcgaaggaag tgtaaacaat aattatacga ttatcgcaac
     2221 agataccgct aagttgacga tcaatccgac tctcttgctt ccttcgacag gtgggatagg
     2281 tctactgccc tttgtctttt taggactgat ctttattttt tctggcttct tttattttat
     2341 ccatcgtaag aaaaaagcag gtgaacagcg atgaaaaaaa caaaaaatgt acgtgtggat
     2401 atatttgctg ttctcttttt tatcctactg gtctttttta taggttctcc gactgtgtca
     2461 gctagagaat cacctattac atttactttg cataatcgaa tgttcgaaag cgataagatg
     2521 ccaagtatca gtcaaaacga cggaataaat atcccggata actttcaagg agtaaaaggg
     2581 ttgaacggcg ttactttcgc tatatatgat gtctcggatg aattttacaa gctgcggtca
     2641 gaaggaagta gtgtggaaga cgcacaaaga aaactagcac aaaaaagtga tagtgaaaaa
     2701 atactggcag aaaatgtgac aaaaacagta gacgaggaag agggcattgc ttcttttagt
     2761 gcatcggaca aagatgagca aggtcgtgac gctgtttacc gatttgcgga aataaagact
     2821 tcagattaag taaaagaaaa atcagcacca tttgtcgttg tgcttcctgt gacagattca
     2881 tctaatcata aattgacgac catccacttg tatccaaaga gcgaacaaaa aatacctgct
     2941 gcactcacat taacaaaaac agtagagaat aagcagacag attttgcaga tggggataaa
     3001 gttccttatc tcatcaccgt aacaattcct gagaacatca acgagatagg aacctatacg
     3061 atcaaagata cagctgatcc tcagttatgg ctagaaccag aaacgatcga ttttttgatt
     3121 ggtggagaca agattcatac atttcacaca caaaaaacaa aacatggttt tgtcttatca
     3181 gattctggaa aagatcttag ttcgtttgct gggaaaaaat taacgatccg ttaccaaatg
     3241 acattgaaag aaaacagtga aaaaacgaca tttgataacg atgtacgttt gacgactgac
     3301 aatcaaacag tagaaacaca tcttgccatc caaacaggag gcaaacaatt tgtcaaagtg
     3361 gatcggcaaa cgaaacaaaa actagcagat gctcaatttc tagtcaaaaa aaatgaccaa
     3421 tacttagcgc aagaaaacgg gataaatcat tgggtagcca aagatgattc aagagcaacg
     3481 atttttactt ccagtaaaga tggaacattt tctgtccatg gattgtcttt tggaagttac
     3541 gaactagtgg agattaaacc gcctaaagga tatatattga gtcaatcaac cattctcttt
     3601 gaagttgaaa aaggcagtta tgatccgctt catcctatac cattggaaat catcaacgaa
     3661 ccaaaagcaa atccgacgaa taatggaaaa acgccagttc agatgcagac aaatggtcca
     3721 tcaaatgaaa aaaatggagg aagctttcca aaaacaagcg aagagatgct tggaggtttc
     3781 tcaattttag ggttgttgct agtcctttca acagggacag cttggtttta taaaaaacaa
     3841 caaaaaggaa acaataggag ggaaataaaa tgaaaaacat caaaacaaaa atatggacgt
     3901 ttttaggaac tgctatcatg ctgcttcctt tcgttctcgg cctaggaact gcagaggttt
     3961 ctaggccgta tcacctacac cagaaaatgt tacggttaat ttgcataaat tgaaattcac
     4021 ttcagcacca gaaaatcaaa taaacaacgg aacggaattg acatttccga atagcgaacc
     4081 gttgaacggc gttgaattca acgtatatga catcactgct acgtactatc caagtaaaga
     4141 cacagcagtt ccagcagacg ctacaccctt cgcaagtgta acgacttctg gtgaaggatt
     4201 agctaacctg actcttccag gtaaatctga tgggaaagat gctgtatacg tctttgtaga
     4261 aacaccaaag cccggtgtcg aaacgtcgcc aaatatcgtg ttaagcttac ccttttataa
     4321 cccgaattca tcagaccctg acaaaccttt agacacgatt cacctatatg ctaaaaatgt
     4381 catcaaagaa agcaatatca aaatccagaa agctgggaac ttttcagggg tagataagtt
     4441 agcaggggcg caattccaga tcaaaaattc agagaatcaa ttcgtgaccg gttttgatga
     4501 aaacactggt gtcgctctct atgatcctgc tgagtcagcg ttgacattca caactgatac
     4561 tgaaggatcc atcagtgtca atggattaat agacggcaat tatactttag ttgaaacaaa
     4621 agcaccagat ggttatcaat taaccaatcc acaaaaagaa actactttta tagtaaaaaa
     4681 cggaaaattg caacaaacaa ccggtatcat ccatggcgca gatgaagcca atcttgggaa
     4741 tccaaacggt gtagtcaatc gggaaaatac aactacggat aacacgatta ctgtagaaaa
     4801 tgtccaaaac tacggtgatt tccacttcat caaacaagat gtgaacacgc aagaaaaatt
     4861 agccgatgca gaattttcta ttctaagagc aaaagatcca actgttaaat taatgaaaaa
     4921 aatagcaaat cttgcctcag gagaataccc atatgcttgg tcagatgaag tcacaggttc
     4981 tgactgggaa gaagtgaaat tgatttctga ttcagaaggg aaattttcaa tcgaagggct
     5041 gaaacaaggg gcatactgcc ttcaagaaac aaaagcacca gaaggctacg cattacctaa
     5101 gaatgaagct gcgtacactg aatttacagt agatgatgat ccaacaactt ctgatgaaac
     5161 aacgatcgat aaccaaccta aaggaatctt gccttcaact ggtggtatgg ggatcatcgt
     5221 attcatcttg gtaggtactg ctttagttgg aggagcagtg atctacttca aaaaacgcca
     5281 tcaagaaaca gaagcttaaa ctattggaaa caaaaatcat acgataaata aacgatagtg
     5341 attcaaatgg ggagaaaggt tgtgaca
//
LOCUS       DAYYKN010000162         5343 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000162 DAYYKN010000000
VERSION     DAYYKN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.164"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACHBB7_002609"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACHBB7_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACHBB7_002610"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACHBB7_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2092..3132
                     /locus_tag="ACHBB7_002611"
     CDS             2092..3132
                     /locus_tag="ACHBB7_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM9210501.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3956..4288
                     /gene="traE"
                     /locus_tag="ACHBB7_002612"
     CDS             3956..4288
                     /gene="traE"
                     /locus_tag="ACHBB7_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM9210502.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="ACHBB7_002613"
     CDS             4907..5248
                     /locus_tag="ACHBB7_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM9210503.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1772 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattga
     4861 tataaaacat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 ttt
//
LOCUS       DAYYKN010000163         5322 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000163 DAYYKN010000000
VERSION     DAYYKN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.165"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..1076
                     /locus_tag="ACHBB7_002614"
     CDS             30..1076
                     /locus_tag="ACHBB7_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9210504.1"
                     /translation="MSVKISVIVPCYNVEKYIDECINSLINQTLKDIEIILLNDGSTD
                     NTLAILKKYEKLDSRIKVYEHKNMGLGPTRNRGIKIANGEFLAFVDSDDFVSNTMFES
                     LYNCAIAENADIAIGEVGIFSDREIERVKIRKSLTNLKTIELATYNKKEFFKQFYFNR
                     LYSHNAWDKIYRRSLVLEFNLSFGDNKIIFAEDNWFQLQVFMTNTRICFVDKLCYYYR
                     QQNNSIMHKPKSNLVFRHGNMIADYEKLIRQNGNNIIDKKACMLVAFDVLIMEIFNQK
                     NVGGTCEDYKNALVSLKHHKILRENISNIVRMKAYELEPNMRKKLALVTISIMFNFKM
                     YKTAYNMLWRIYNR"
     gene            1090..2169
                     /locus_tag="ACHBB7_002615"
     CDS             1090..2169
                     /locus_tag="ACHBB7_002615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9210505.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAKKYFSKINDKKVK
                     TIIIPNPIKENLPYYNKTTSSKKFITACRLDKQKNLPLMIDAIGKLIHEGYECELDIY
                     GEGGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEAL
                     AIGVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQK
                     GIKLRKELTSKNISNIWYEYLNQFF"
     gene            2188..3612
                     /gene="wzy"
                     /locus_tag="ACHBB7_002616"
     CDS             2188..3612
                     /gene="wzy"
                     /locus_tag="ACHBB7_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFM9210506.1"
                     /translation="MKEKINILMFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAIC
                     TWHWRKGDSIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSES
                     DMIMVKWYSCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIV
                     VPIVFVSKLLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKV
                     ITRFVFIIFALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIG
                     IYILNVITKARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNG
                     IYPFANTYLTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNG
                     GYIGGIVYIFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIV
                     KNFVYGVLIILLINFFLQQFNKRL"
     gene            3658..5163
                     /locus_tag="ACHBB7_002617"
     CDS             3658..5163
                     /locus_tag="ACHBB7_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM9210507.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAVIVFICIILVNVLFYRRYVVKIISKIIVKIKKI"
BASE COUNT         1903 a          621 c          952 g         1846 t
ORIGIN      
        1 tataacaaat tgataaagag gtaataataa tgtcagttaa gattagtgta attgtacctt
       61 gttataatgt tgaaaaatat attgatgagt gtataaatag tttgataaat caaacattga
      121 aagatattga gattattttg ttaaacgatg gttcaacaga taatacgttg gcgatattaa
      181 agaaatatga aaagcttgat tctagaataa aagtttacga acataaaaat atggggttag
      241 ggccaactag aaatcgaggg atcaaaatag ctaatggaga atttttggct tttgtggatt
      301 ctgatgattt tgtttccaat actatgtttg aaagtttata taattgtgca attgcagaga
      361 atgcggacat tgcaattgga gaagtaggca ttttttctga tagagaaata gaaagagtaa
      421 aaataagaaa aagtctaacg aacttaaaaa caattgaatt agcaacatat aacaagaaag
      481 aattttttaa gcaattctat ttcaatcgtt tatattcaca taatgcttgg gataagatat
      541 atagacgtag tttagtatta gagtttaatt tatcatttgg tgataacaaa ataatttttg
      601 cagaagataa ttggtttcaa ttacaagtat ttatgacaaa tacaagaatc tgtttcgtag
      661 ataaattgtg ttattattat cgccaacaaa ataattctat aatgcataag ccaaaaagta
      721 atttggtttt tagacatgga aatatgattg ctgattatga aaagttgata aggcaaaatg
      781 gaaacaacat aatagataaa aaagcttgta tgttggttgc atttgatgta ctaattatgg
      841 agatttttaa tcaaaaaaac gttggtggaa catgtgaaga ttataagaat gcattagttt
      901 ctttgaaaca tcataaaata ttgagagaaa atatttcaaa tatagttaga atgaaagcat
      961 atgaacttga acctaatatg agaaagaagt tagcccttgt gaccattagt ataatgttta
     1021 attttaaaat gtataaaaca gcgtataata tgctctggag aatatataat cgataaagag
     1081 gtaggaaata tgaggaaatt gacctttgtg ataggaatta tgggaaaagg aggggcagaa
     1141 cgagtaattg cgaatttggc aaataatttt attcaagata actgggaaat tgatattata
     1201 actatatatg ggaatcggca agactatgaa ctgaatagaa aaatacaggt acatccaata
     1261 atatgtaaaa gttcgtttcg tgcatttaga cctttggaac gctttaagga acttaggaaa
     1321 aaaataaggg aaatacagcc tgatctggtt atttcgttcc ttgctgatgt aaatatccat
     1381 gtcattattt cattgttagg agtaaaacaa aaattaattg tttctgaacg aaatgatcca
     1441 caacggaatc cagaagaacc atggattagg aaaattagag attttcttta taaaaatgta
     1501 gatggaattg tttttcaaac accagacgca aaaaaatatt ttagtaagat caatgataaa
     1561 aaagtaaaaa caattataat tcccaaccca ataaaggaaa atttacctta ttataataaa
     1621 actacaagta gtaaaaaatt tattactgca tgtagattgg ataaacaaaa aaatttgccc
     1681 ttaatgattg atgcaatagg aaaacttatt catgaaggat atgagtgtga actggatatt
     1741 tatggagaag ggggattaaa aaccgagctt gaaagttata tcaaccataa tggattaaca
     1801 gaatttgttc atctaaaaga atttacaaat aaaatacatg aaaagatgca aaatgcggca
     1861 ggatttgtaa tttcttctga ttatgaaggt atatctaatt ctatgttaga agcactggct
     1921 attggagtgc cagttatcgc tacagattgt ccagtaggtg gcgcaagaat gtatttaggt
     1981 gatagaaatg aaataggtca tcttgttgaa tgtaataatc tagataagtt aacagatgca
     2041 atgcgtaggg tacttttgga tagagaaagt gtggtaagaa aagcgcaaaa aggaattaaa
     2101 ctaagaaaag aactgacatc taaaaatatt tcaaatatat ggtacgaata tttaaaccag
     2161 ttcttttaac caaaaggagg atattgtgtg aaagaaaaaa taaatatatt gatgttttcg
     2221 ttaacgttag tcgcgtttac aatattttgc gctttttcat taaatcagaa ctattggcta
     2281 aattggcttg gaatattgat ttacttatat gcaatctgta catggcattg gagaaaagga
     2341 gattcaatat tttcattata cacgattttt ttcactcttt ttttattatt tagttatggg
     2401 caatgcatta tgtgggcatt cggtattgga acagatcgtg gaatcggtac aacagtagta
     2461 gcttatggta cggggattat tcctagtgaa tcagatatga taatggtaaa atggtattct
     2521 tgtatttcta tgtttgtatt tcacatagga gcactcttat ttacgagaaa aaccacacta
     2581 agaaaagtta gttggtatga atctggagat gctgatgccg atagaaagtt tttatttaaa
     2641 atagggtgta tcatttctat aatagtagta ccgatagttt ttgtttctaa attactagag
     2701 gtacgtatag cattagtcca tggttataat gcattatatt atggtgatta tgctacacaa
     2761 agtggatatt tacaaattat tctatatctt ttttttccag ccttaatatg tttattaatt
     2821 ggtagcaact tttcaaaagt aataacgaga tttgttttta ttatttttgc tttatattct
     2881 ttattaggaa ttatgtcagg agacagagga agttggttgt atagctctat tatactcata
     2941 tgggcataca ctcaatataa aggaaccttt aattataaaa aattaataaa gtggcttatt
     3001 ttagctgtta taggaatata tattctaaac gtaataacga aagcacgtga tggtggagga
     3061 ctaagtaaac ttactttaaa agattttaca tctgtatttg attcagatga ttctccttta
     3121 gttgattctt tttttgaaat gggggggact atgagtatta taactttttt cctaataagt
     3181 ggtaatggaa tatatccctt tgctaatact tatttgacct cactcttagg ctctatatca
     3241 acaagaatgc tatcaatgtt tggaataaag tttgttttat tagctgattg gttttctcag
     3301 gattatttag gaatcacttg ggggactgga ttctcaatga ttgcagaagc ctatgtcaat
     3361 ggtggataca ttgggggtat agtctatatt tttattattg gtatagtcat cggtaaaatc
     3421 ctttctaata gtatagacaa ccaagatatt gaaaatcatc ctatgaagat gtttttggct
     3481 ataactacag caaatattat aatgggtttt cctagagctg cttcatattt aattgtgaaa
     3541 aattttgtgt atggtgtttt aattatattg ttgataaact ttttcctaca gcaatttaat
     3601 aaacgtcttt aatatttaag cttaataaaa agataaaaca agtcaggagt tgcaaaaatg
     3661 gaaagaactg ctcggctcaa aaaaaacact atttgggctc tggtgtttca aataacttct
     3721 attgtctgtg gtatgatctt gctacgtttg atattaacaa agtacggatc cgaggttaat
     3781 ggaatgatta gctctattaa ccaatttcta caaattattt cttttttaca attaggagta
     3841 ggcgctgtaa tgcaatcaac tctatacaaa ccactagcag aaaaggataa tttaatgata
     3901 agtcaaatct tagcttctgg agggaagttt tatcggcaaa ttgcatatat tctactagta
     3961 tacgtgggtt tgttacttgc agtctattat gttctcgttc ctaatgaatt tgggagatca
     4021 tatacaatgg caatgattgc aacattaagt attaattcat ttgctcaata ttattttggt
     4081 attgttaata ctttgttatt aagtgctgac cagaaaattt atattacgta tattgcacag
     4141 acaatagcat ttatttctaa tacagttgca tgtgctttat taattagtct gaacacagag
     4201 atccttatgg taaaacttat tactgctata atttatcttg cttgtccaat atattataga
     4261 tggtatgtgg atcgaaacta taatataaag aggaatgtgt catatgataa ggaaccgatc
     4321 cctcaaaaat ggaatggggt tgcacaacac gtagcttctg ttgtactaga tagtactgat
     4381 gttgtagtgt tatctatatt ttctaacctg atatatgtat ctatatataa tgtgtacaat
     4441 ttagtaatat acggagtaaa acagctattc atggctatga caaatggcgt tcaggcctta
     4501 ctaggggaac tttgggtcag aaaagaaata gaagttcttt acaaaacatt tgcatggttc
     4561 gaatggattg tacatacttt gacagttttt atttttggct gtaccggtat actaatcgtt
     4621 ccatttattc aagtatatac aagaggagta acagatgcta attataatca attatcattc
     4681 gcagctttac ttgtaactgc taacgcactt cactgtctgc gtacacctta tcatgtaatg
     4741 ataaaagcag ctggtaaata taaagaaact caaaatagtt ttataattgc ttctttgtta
     4801 aatttaggtt tatcattaat tttggttaaa ccactagggc tagttggagt agcattagga
     4861 tctctaattt caatcacata tcatacactt tggagtgcaa attataattc aaaatatttg
     4921 cttaaatggc cgatgaaaaa attctataaa cagttatttg taaacatctt aactgtagtt
     4981 ctttcttttg gggcgacatc acaattgaaa atgatgtcaa tcacatattt ggattggatt
     5041 gttctcgcaa ttaaatgtgc agttattgta tttatatgta taatcctagt taatgtttta
     5101 ttttatagaa gatatgtagt aaaaataatt agtaaaatca tcgtaaaaat aaagaaaatt
     5161 taatacacaa taaatgtttt aataaactta ataaaattat atttgccaaa attagacaca
     5221 tatattaaat tttttctttt aaggttactt atgtgatcaa gaataatttc tattcgtttt
     5281 agaataagtt aatagataat agaaagaagt gggttgctta gc
//
LOCUS       DAYYKN010000164         5311 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000164 DAYYKN010000000
VERSION     DAYYKN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.166"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(72..1220)
                     /gene="nagA"
                     /locus_tag="ACHBB7_002618"
     CDS             complement(72..1220)
                     /gene="nagA"
                     /locus_tag="ACHBB7_002618"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM9210508.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1296..2132)
                     /locus_tag="ACHBB7_002619"
     CDS             complement(1296..2132)
                     /locus_tag="ACHBB7_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210509.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2316..3533)
                     /locus_tag="ACHBB7_002620"
     CDS             complement(2316..3533)
                     /locus_tag="ACHBB7_002620"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9210510.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3682..3843)
                     /locus_tag="ACHBB7_002621"
     CDS             complement(3682..3843)
                     /locus_tag="ACHBB7_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210511.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3916..3999
                     /locus_tag="ACHBB7_002622"
     tRNA            3916..3999
                     /locus_tag="ACHBB7_002622"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3950..3952,aa:Leu,seq:gag)
     gene            complement(4239..5311)
                     /locus_tag="ACHBB7_002623"
                     /pseudo
     CDS             complement(4239..5311)
                     /locus_tag="ACHBB7_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1737 t
ORIGIN      
        1 tttactagaa cacaaaaaaa accttaaaac caaacttaag aagtttagtt ttaaggttaa
       61 cttaaattat attatgcgtg gaaacgttcc acgccatcta gataagttgc ttccaatgtc
      121 atatcagggt tcaatacgat aaagtctgca tcgcgtcctt gagcgatttt tccacatgta
      181 tcatcgattt tacagctgat tgctggaatc agactagcca tcatgactgc ttgttctggt
      241 gttgctaatc cccagtctac aacattttta attgcttctt tcaatttcaa tatacttcca
      301 gctaagttgc cagattctag acgtgctgtt ccttctttta ctactactgg aaattcgcct
      361 aaattgtaat ttccatcagg catcccaccg gccatcatac agtctgtaat taatgcgaca
      421 tgatcatgtc ctgctttttc cattaggatt cccgcagcat ttggatgtac atgatggcca
      481 tcacaaatca attcagaaaa gacatgttct aaagataata acgcacccac catacctggt
      541 tcgcgatgat tcaatccacg cataccattg aaagcatgga caaatacact tgcgcctgct
      601 tcaacagctt tttgcgcttc ttctagtgta gcattactgt gtcctagaga aacaactact
      661 ccttcttcag tcacttgttt gacaaactct tctacacctt gacgttccgg tgctaaagca
      721 atttttttga ttaaaccacc tgaagcttct tgccattcat ggaaagtatc caaatcagga
      781 tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt ctgtgaagaa aggaccttca
      841 aaatagattc ctttgatttt agctcctggt gcttcctgat acattttgcc gattgtctca
      901 gctacatctt ttaaacgttc tttgcttgac gtcaatgttg ttggtaaaaa tgaagtcact
      961 ccacaagata acagaccttc agacatcact ttgatgcctt ctgcatcatt atccattaca
     1021 tcatgattca tatagccgtg aatatgtgta tctaccaaac caggagcaat ccatttgccg
     1081 ctttggtcaa tgatgttgac ttctccctca ggtagttctt tggtgtattc accaaaaagt
     1141 ccatcggtaa tttcaagata gcctgcatgt ttgacacctg acgctaaaaa gaatttgtca
     1201 gcatagataa aagttctcat tctattctct ccctttgttt ttctacctct aaagtactct
     1261 tatttagaga agaagtcaag aatggtctac accatttaca aattttttga aatttgtttc
     1321 acagcttgtg ctaagccttc atagaatgct gctctttctg gttcttcttt acgcttgtat
     1381 attaagcttt taagcacaaa taaatcattg aggtaatagt agctattctt ctcttgagcc
     1441 caatagattc cttgattcac ctgttctaac gcttcgtcga tttcattttg atggaataag
     1501 aaatttccat aattataaaa aatttgcgaa aattcacttt ttgatcgttc atcagctgat
     1561 tgatgcagca gttggatgct tcgactcaaa tagtagcgtg cttcagccgt ttttcctgcc
     1621 acaccatata tttttccgat acaactaatc agttgtattt ctgtgtcaga aacatggatt
     1681 ctttcttttt ggctagagta gcttaatgct tctttcaaat aataaaggga ctgagagata
     1741 ttttgacgca acgtgaattc gcaaacaccg agataataat aatagcgttg aaagtcagtt
     1801 gcttgataca gattttcaga aatatcagaa gattttagta cttgttctaa cttccgataa
     1861 tcacgttttt ggaaacaact atccaacagt tcaaataatt tatcgtttgt ccgagtagtt
     1921 tttcgagaga gattcatcac atgatcaatc gttaccccta gccgttgaca aatctgcatc
     1981 atcaccaata cattcggcag ttcctcatta ttttcgatcc gacttaatac actttgtgaa
     2041 caaatatcat gcgaaagcat tttttgcgtc attttttgtt cttttcgaat ctctttaatg
     2101 atcgttccaa aggactcgat taacgtttcc ataagtatcc tccccaaaat atgcgtattc
     2161 gcattctatt cgtattttac tttaggaagt cgcaaacttc aagaaaagac ttgcaactaa
     2221 ataatatact ttagttattt aagaaaactc cactagaaag agagcctcag tgtgtttgtc
     2281 tgtggccctc ctactgaaaa atttggtttt taaaatcatt tttggcgata ggttgctagg
     2341 aattctctca ttttcttagc tgtttgtttc aaatcatcca ttttaggaag ataaacgatt
     2401 cgaaaatgat ctggctggtt ccaattaaat ccaccaccat gtacaagtag aatatgatgt
     2461 tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa tattgaatcg ctttgtatca
     2521 atttttggga atatataaaa agcagctttt ggttttacgg ctgaaagacc aggaatatca
     2581 ttgattgcat tatagatata ttctctttgt tcatagatcc ttccgcctgg caggagcagc
     2641 tcatcgacac tttgataacc gcctagcgcg gtttggataa tttgctgaga caatacattg
     2701 gaacacaaac gcatggagga caacatattc agcccttcta tatagccttt gacgtgtgac
     2761 ttatctccac ttaggaccat ccagcctact cgaaatcctg ctacacgatg agatttggat
     2821 agaccgttca gcgtaacaac gaacaaatca ggcgctaatg tagcaatcgg aatatgttct
     2881 agaccatcca tcactaagcg atcatagatt tcgtcggaat aaataatcag gttattttgt
     2941 cgagctactt caacgatttc ttctaataat tcttttggat aaagtgcccc agtaggatta
     3001 ttaggattaa tgataacaat agcttttgtc cgactcgtta tttttgattt gatatcatca
     3061 atatcaggat accattctgc ctcttcatcg caaatataat gaacaggtgt gcctccagct
     3121 aatgcaatag aagccgtcca caacggatag tccggcattg gaaccagtac ttcatctcca
     3181 ttgtccagca acccctgcat acacatcgtg atcaattcgc tcactccgtt gccggtgtag
     3241 atatcattga tcgtcacatt cggaaaatgt ttcagttggc aatattgctc aattgctttt
     3301 ctagcagaaa agatcccctt ggaatctgag tagccttctg agtcacgggc attcatgatc
     3361 aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag gtgcgggatt tcccgtgttc
     3421 aattttaaaa tactaatgcc ctcttcttgc atccgctccg cttcttctag aacgggaccg
     3481 cgaacatcgt aactgacacc ttctaatttg ttggattttt caaattctct catcacttag
     3541 ccaatccctc atttcaaaaa gttagaactt tccaaatttt ttgttattta ctatttcaat
     3601 atcatactcc atcctagaaa attttgccac ttatttagga aaaacgtaag tattaaaaaa
     3661 acacaaacaa gcgttgagaa cttaattgtc atgatagtca ttccatagat tcgctgccca
     3721 atccattgca attccacaaa tgattcctag cataaaacca agcaaaaaac gtccgaatgc
     3781 aagtccaatc aatacaccta agatcattcc cagtcctgaa aattctccgt catatttctc
     3841 catcttaata agtcctccca tttttgagct tgacaactat gtaaaacctc gctatcatta
     3901 gtgtgttatt attacgcaga tgtgttggaa ttggcagaca agcatgattg agggtcatgt
     3961 gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac tacaactcgt gaagttaatt
     4021 ttgcttcacg agtttttttg tttgaacgtt tctttatgcc attatataca tatcactgta
     4081 tcctttacaa aaagaacgta caatcatgta aacagcctga ggtgagtata tgaaggtcgg
     4141 aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat taatcgaaaa acgagtaaaa
     4201 tccctcacct aatactaaag cgagggattt tttgtatatt atctgtttat caccgttatt
     4261 ataaaggtac aaataccaag cacggttaat ccaaattgca taaattcatt tttttgtaca
     4321 ctcatgcttt tcattagctc tttaactgcc attagtacta agatggcgat aacaacataa
     4381 agtatatagt acgtccgcat tccggaagcc catactgtgg gagaaaatcc cataattaac
     4441 cttgaacaaa atcccattat catcaaaata actaaaaaaa tagaagtttt cttattgtca
     4501 aaacttaaat aaattccaac aattaaacat agtaataatg ccaagactac taaagtagct
     4561 atccaagtac ctggataaaa aagtgacagt ttcgttccta actctgtaaa gagattattc
     4621 aggttactac tactccaaat cattgccctt ttatttccat tgacgtaagt aaagctttga
     4681 cccattgtat taccaaaata aataatcagg tttaagaaaa acggtaatgc agtcaatata
     4741 cgcacataat agttttgaca tttcctatat gttagaagga aaattaagaa aaatagcaat
     4801 aaaaataaga tattcatgtc taaaaaaacg gctttccaaa tgatgaaata cctaaatcta
     4861 acttattaag aaagttgaaa tttttatatt caggaaacca tttattaatt tcatgtgcag
     4921 cacgtaatgc attccctgga gttgtcaaag agaaaattaa ttctgctaag cttattattg
     4981 aaaatactga taacttataa ttatatcgtc cattgacaat caaatataaa ctaactatac
     5041 ttgttagaac aaaaaaacag gcgttgactt gttcttgatt agttaaaaat atcaataaag
     5101 gaatacttac ccaatatatt tttctattta tttcttctcc tcttaagagt tgaaaaaacg
     5161 gataaaaagt tagtaaacta aatgctactg gccactgata gttcaaggtg gttgctctcc
     5221 aaccggtttc ccccattact gtcaatggta ttaaacagta tatagagcaa accaataata
     5281 aatttttaac atttattttc tcaaaaacat a
//
LOCUS       DAYYKN010000165         5207 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000165 DAYYKN010000000
VERSION     DAYYKN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.167"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1368
                     /locus_tag="ACHBB7_002624"
     CDS             <1..1368
                     /locus_tag="ACHBB7_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210512.1"
                     /translation="SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIAL
                     TIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSK
                     QIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDS
                     IIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFL
                     FQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIA
                     AKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGE
                     SWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVD
                     VNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINK
                     KNENK"
     gene            1355..2989
                     /locus_tag="ACHBB7_002625"
     CDS             1355..2989
                     /locus_tag="ACHBB7_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM9210513.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3164..3997
                     /gene="msrA"
                     /locus_tag="ACHBB7_002626"
     CDS             3164..3997
                     /gene="msrA"
                     /locus_tag="ACHBB7_002626"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9210514.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4077..5066
                     /locus_tag="ACHBB7_002627"
     CDS             4077..5066
                     /locus_tag="ACHBB7_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9210515.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1825 a          806 c          951 g         1625 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttggtctgtt
     2221 atctttatgt attcccctga ttttatttaa aaaattaaca aacatagaaa aaaagattta
     2281 ttatggtacg tgtttgactt ggctagctac aactagtttg gtgccatggt atcttctgca
     2341 aaatacgcca gccaaattgt tacaatttcc ttggcggatc ttaagcttgc aaataatatt
     2401 ctcttctctg atattaacga tgatattttt caaaaataga agatacaata agacaagaga
     2461 actattttat ttatttatga ctgttttgtt attaatcact ttaactgttt ctagtaaaaa
     2521 aaatttcaat caaaaaataa tttctcagcc gggacatatt gttgtaaaca aagaaacagt
     2581 agaggcgtat accactaaag gaatggatta ttttttatat gattattctc ctatcgcttc
     2641 attggagaat aaggagcata taataaaaca cgaagcaaag attggcagtc aatgggtaaa
     2701 ccttccttat aaaacaactg gtagttctat catatataga gtaactaatg agaagagcca
     2761 agtaatcaca cttcctgtat atgcttattt aggaacgaat gtagaaataa atcatcaacc
     2821 tgtaaaggag caggtgaatg ctaatggatt ggtacaaata agcggagtca aaggagttgc
     2881 tgatattaaa gtaagcacaa gttatacagt aacgactatt atttcttatc ttgttacatt
     2941 aactgcatat ttatgtttat tttttaaaat agttttccat aaaaggtaaa ttaacttttc
     3001 atttataaaa taaattgatt aaaaaggaga atctgtcgat aaggattctc tcttttttat
     3061 acgaaaaatc tgtaggggga tgaagtaatc gcttatttcc tcactttatg ataaattaca
     3121 gctaaagtca ctagcttgtc ttcttcggaa aggaggagaa tctatggatg aggaacaaat
     3181 attcattcaa acactttata atctgatttt gaatccgaat acacgagatt gggaacgaaa
     3241 agtattgatt cagacaaaga atgacataag agaaaatata tccgttaaag aacagttatc
     3301 aaaactagaa gcaacactac gtccattagc tatccgaatg aatctgacgc ctgatgtaat
     3361 ggacttttat ttattgatta cggaaggatt tgataaggag caaaaatatg acttttccaa
     3421 gcatgccatg caagatgctg actatcaaga acgtgcagta tttgcagggg gatgtttctg
     3481 gtgtatggtc gagccgtttg aatcgaaaaa agggatccta tctgttttgt caggatacac
     3541 gggcggacat gttgaaaaac caaattatga tcaagtaagt ggtggttaca caggtcatgt
     3601 ggaagctgtg gagatcattt atgacacaag agaaataagt tatagcgagt tgctgacaat
     3661 ctattggcaa attactgatc ccacagatac atttggacaa tttcaagata gagggaagca
     3721 atatcgtcca gtgatttttt atcaaggcga acgacaaaaa gaattagcgg aacaatcaaa
     3781 acagaaacta gacagttcag gaactttcca ccaaccaatc gtcacaaaaa tcgaaccagc
     3841 cggtacgttt tggccagcag aaaactatca tcagcaattt tacaaaaaac aaccgaaacg
     3901 ttataaaaaa atccagcaag cacgtaatca atttctgatc tatcaacgag taaaaaacaa
     3961 atggcaaaaa aatatacgaa aaaatcattt tgattaactc cttttcttta aaatgtagta
     4021 aactaaaaat agaaaaaaga aagcgtttta aaaagatttg atgaggagga gaaaaattgt
     4081 atattgcaga tagtgcacgt tatgaaaaaa tggaatatcg taaatccggt cattcaggtt
     4141 tgaaattacc gattttatct ctcggattat ggcaaaattt tggtgattat gatcctatcc
     4201 ataatcaaag agagatttta agaggcgcat tcgacatggg aattacccat ttcgaccttg
     4261 cgaataatta tggaggccct gctggagctg cagagaaaaa ctttgggcgt atttttagag
     4321 aagattttca agcctaccgt gatgaactga ttatttctag caaagcaggt tatcatatgt
     4381 gggaagggcc atatggggaa tggggctctc gtaaatccat catttcaagt tgtgaccaaa
     4441 gtctccaaag aatggggcta gattatgttg acatcttcta tcatcatcgc ccagaccctg
     4501 atacaccttt ggaagaaaca gcagaagctt tgatgcaact tgtaagacaa ggaaaagcat
     4561 tatatattgg tatatccaac tataatggag aagatacaaa aaaaatgact gaaattttaa
     4621 aaagaaagga agcaccgttc attattcatc agatgcgcta caatatgttt tctcgtgcac
     4681 tactagaaga cgatctatct ccagtattag aagaagaagg gctaggggca atcacgttca
     4741 gtccgcttgc tcaagggttg ctgacaaatc gttatctaca tggtattcct gaagattcta
     4801 gagcacatcg taaggaaata ccattccttt cagaagaaca agttggatca actctggaaa
     4861 aaatcaaagc actgcaaaca atcgccgtat cacgaggaca atcattagcg caaatggccc
     4921 ttgcatggaa tttgcgacaa aaatctgtta caagtgtact ggtcggagca agtcggttaa
     4981 gtcagttaca agaaagtgtt cgaatgatgg ataatttaga tttctcacca gaagaagaat
     5041 tgagaatcga ccaaattcta gaatagaaac gatgtaatca aactttttat ccatatatta
     5101 aaaaaaacaa aagatgacat caagaatcga atgaagatcg tctgttattg aacatcatat
     5161 tttttgtcgt tcaaaataaa aatgatcgaa ggctaggagt gaatttt
//
LOCUS       DAYYKN010000166         5199 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000166 DAYYKN010000000
VERSION     DAYYKN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.168"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..696
                     /locus_tag="ACHBB7_002628"
     CDS             25..696
                     /locus_tag="ACHBB7_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM9210516.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACHBB7_002629"
     CDS             755..1195
                     /locus_tag="ACHBB7_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210517.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACHBB7_002630"
     CDS             1207..1875
                     /locus_tag="ACHBB7_002630"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM9210518.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACHBB7_002631"
     CDS             1939..2562
                     /locus_tag="ACHBB7_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM9210519.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACHBB7_002632"
     CDS             2618..3004
                     /locus_tag="ACHBB7_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM9210520.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACHBB7_002633"
     CDS             3217..4608
                     /locus_tag="ACHBB7_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM9210521.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1728 a          861 c         1095 g         1515 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacag
//
LOCUS       DAYYKN010000167         5017 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000167 DAYYKN010000000
VERSION     DAYYKN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.169"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..165)
                     /locus_tag="ACHBB7_002634"
     CDS             complement(<1..165)
                     /locus_tag="ACHBB7_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9210522.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFK"
     gene            complement(367..762)
                     /gene="panD"
                     /locus_tag="ACHBB7_002635"
     CDS             complement(367..762)
                     /gene="panD"
                     /locus_tag="ACHBB7_002635"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFM9210523.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(772..1620)
                     /gene="panC"
                     /locus_tag="ACHBB7_002636"
     CDS             complement(772..1620)
                     /gene="panC"
                     /locus_tag="ACHBB7_002636"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFM9210524.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            complement(1635..2462)
                     /gene="panB"
                     /locus_tag="ACHBB7_002637"
     CDS             complement(1635..2462)
                     /gene="panB"
                     /locus_tag="ACHBB7_002637"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFM9210525.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(2474..3334)
                     /locus_tag="ACHBB7_002638"
     CDS             complement(2474..3334)
                     /locus_tag="ACHBB7_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFM9210526.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            3572..4741
                     /locus_tag="ACHBB7_002639"
     CDS             3572..4741
                     /locus_tag="ACHBB7_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9210527.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT         1611 a          942 c          633 g         1831 t
ORIGIN      
        1 tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag aaggcgtaag
       61 ctcatactct cgaataattt ctgcgcgagg cttaccagcg agatagagat cgacgatttg
      121 ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt gacatgaaaa atcctccagt
      181 gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta gccgattcac
      241 ataaaatgga ctttgtctac agtctgtatc ttctcttttt gtgtctactt tattgacatc
      301 tatccaatta gtaatcaata aatatttcaa taatagtcta gctatatata aataacatta
      361 caaaacctaa agaattatat ctgataacct tccatgtttt tcatatgatg ttactctttc
      421 aactttatta ttatcatcga caaaaacaac cttaggtcta tgatccttaa cctcattttc
      481 atttaattca caatatgcca ttaaaattat tttatctcca gttgatacac atctagctgc
      541 agctccattc aagcaaatca tccctgttcc tctctctcca gctattgtat aagtttcaaa
      601 tctatttcca ttattaacat caactatttg tactttttca tattcatata ttcctgctgc
      661 ttctaataaa ttcatatcaa cagtaatact tcctacataa tctaactcag cttgttcaac
      721 tgtagctcta tgtatttttc cttttaacat tgtaactttc attatagccc cctattgtaa
      781 ttcaaatgta aaattatcta ttaatcttgt ttttcctata aatactgcta ttgcaactaa
      841 aactgatttt tcaatataat ttactctttg taatgataaa gaatcaacaa cttctacata
      901 atcaatcttt gccaaattat aagtatttat atttttactt attatctcaa ttatttttaa
      961 gctgtctctc tctccattat ttaaagcttc cttagctaaa gttaaagatt tatttaaaat
     1021 agtagctgaa gatctttcct ctaaacttaa gtatgtgttt ctagaacttt tagctagtcc
     1081 atcctcttcc cttataattg gacaccctac aacgtctata tcaatattaa ggtccctaac
     1141 cattctttta attacagcta actgttgtgc atccttttct ccaaaataag ctctatctgc
     1201 tggtattatg ttaaataatt tacttacaac agtgcaaacc cctctaaaat gtattggcct
     1261 actagctcca cataaacctt ctgttaatcc agttatatta acaaaagtac ttgcattgtc
     1321 acaatacatc tcttcaactt caggattaaa tattgctgtt gctctacctc ttgtgcatac
     1381 ttctatatct ctttctaagt ctcttggata actacctaaa tcttcatttt tcccaaactg
     1441 agttggattt acaaagatac ttacaacaac tttatcattt tcttttgatg cttttctaat
     1501 taaactttca tgaccttcat gtaaatatcc catagtagga actaatccta cctttaagcc
     1561 ttgttttttc cactctttta cttgacttct aacatcattc actgttttaa atataaccat
     1621 atttcttgac ccttttaata taatttttct aaaacatcat cacttatttt aaacccatgt
     1681 tctggacctg gaaaaactcc atcttttact tcctctatat attttgtaaa tgctttattc
     1741 atctcttctg ataaattagc atatttcttc acaaatttag gggtaaaatc actatacatc
     1801 cccaacatat cttgatatac aagaatttgt ccatcacacc ctgcacctgc tccaatacca
     1861 attgtaggta ttgatatctc tttagaaatt aactctgcaa gcttagttgg aacacattct
     1921 aacactacag cgaatgctcc tgctttttct acagctttag cagctcttat taattcttta
     1981 gcagcttcct tgtcctttcc ttgtacctta aatcctccaa atgcattaac agattgaggt
     2041 gttaaaccta tatgcgccat aacaggtata gaagccttta ctatagcctc tattttatcg
     2101 catacttcta tccctccttc taatttaaca acctgagctc gtccttcttt tattaatctt
     2161 cctgcattaa ctactgaatc atatactgaa gtttgataag acataaatgg catatctgca
     2221 acaactaaag tgttcttagc tcctcttgta actgctctag tatgatgaat catgtcttcc
     2281 attgttactg aaagtgtatc ttcatgtcct agacatacca taccaagaga atctcctact
     2341 aatattccat ttatcccagc ttcatcaatt atttttgctg ttgaatagtc ataagctgta
     2401 agcattgtta atttttcatt tcttaattta gattctttaa atgtaactgc tgtatttttc
     2461 atctatttct ctcctaactc tctcctaaat acttttctaa ttcactataa tctctattaa
     2521 tattttttaa catagctatc tctaatacat tttttgataa tagcatatat aaagctctgt
     2581 cttcatctga taaaacttca cagtgacctt taattgttga aatatcacaa cgttcaactg
     2641 gccctgtcaa actttctata attccttttt cttttatatt tctaatatta aattcaatta
     2701 atggatataa agcttcaatt gccatttttt cagtaaatcc acattgaatt agatatgtaa
     2761 cgcttgtatt aataagtcct agcacaaggt tactagatac tactgatgcg gcatgataaa
     2821 gagatttatt ctccttagat acaattacag tactattacc taactttttg aagaaatcac
     2881 ataaaaactt tgcatatttt tcatcgcctt caattgttat aaaagatttt tttaattttt
     2941 tgtatgaatt atatttatct gatattgaaa acataggatg gacagaatag ccataagcac
     3001 catagtcctg tatatttgaa aagatatctg aagatattgc accactgcaa tggcagatta
     3061 atttgttttt gatttgataa tttttaattt cttgccatac ctcctgaatt tgaccatcag
     3121 gagtcgtaat gaaaatagta tcattttcat ctactaattc tcttaagttt aaatattgtt
     3181 tactagatgt aaaattcgaa gctgctaatg acgagtcttc gcttttacta taaaatcctg
     3241 ttacttgtat tttatgttcc tggaaatact tcccgaatga acaacctacc ttgccggctc
     3301 caataaaacc aacgttcata attatcacct ccataaagaa gtgagactac aagtcccatt
     3361 cttacgatat gagcttaaaa ataaaaatta aatcaaatat acagtatgtt tttttcaaat
     3421 atcatccata gttaatttat cataatatcc catattttaa aataaaagat agcgtaaaat
     3481 attttgtgta aatagaaatt tagggtagaa aaaaaagtcc attctgtaaa attaaagtta
     3541 ccacacagaa attttatagg aggactttct catgactaaa tttactatag aaattatgca
     3601 aacactactc aataaagggg atttagatga attatttcag gatcatttag agcgagcgat
     3661 taattcactt ctacaagcag agctaactgc ttttttagat tacgaaaaat atgatcgaaa
     3721 tggattcaat tcaggaaatt cccgtaacgg gaattattca cgtacattta aaacagaata
     3781 tggcgagttg aatttaacta ttcctagaga tcggaatggc gaattttctc aacaaacact
     3841 accagcatat aagcgaacca atgattcatt agaaacgacc attatccaat tatttcaaaa
     3901 aggcattact atggctgaaa tctccaaatt aattgaaaaa atgtatggtc accactatac
     3961 gccacaaaca atctccaaca tgagtaaatt ggtggctgaa gatgttttag cttttaaaga
     4021 aagaacgtta gaagccaatt attccgttat atttatggat gcgacgcata tccctgtgaa
     4081 gcgacagact gtttcgaagg aagcagtgta tattacgatt ggtattcgtt tggatggaac
     4141 caaagaagtt cttgggttta ctattgcccc aactgagtct gcttatattt ggaaagaagt
     4201 tcttcaagat cttagaaaac gtgggttaga agaagtttta ttagtagtga cagacggatt
     4261 aagctgtatt gaagaaagta tccatagtgt gtatccgaat gcccaatttc aacaatgttg
     4321 tgtgcatgta tctagaaata tcgctcataa agttcgtgtt cgaggtcgaa aagaaatttg
     4381 tgaggatttc aaattggttt accaagcgaa ttcaaaagaa gaggcattgg atcacatcga
     4441 ctttatgact aggaaatgga aaaagcagta tccaagagtc gtcaatttac tcttgaatcc
     4501 tgccctatta accttttata atttccctca cgccatcaga cgaacaattt attcgacgaa
     4561 cctgattgaa ggctttaata agcagctaaa acgatatact cgaagaaaag aacaattccc
     4621 taatgaagaa tctctagaga gattcttcat ttctcaattt aatcaatata accaaaaatt
     4681 tttaggtaga attcacaagg gatttaaaga aattcaggat acattagagt cgatgattta
     4741 actgtaatta acagaatgga ttttccattt acacataatt cttgacgcta ccataatcta
     4801 aataaccatc atattcatct actacttata taattgagtt ttattaaact ttttaagttt
     4861 ctattctatc aaatatttaa acaatctctg catccgaaaa gactacctcg tatagtttta
     4921 ctaacaatat ttttattagc gtgttcaaat cctgatttct tgtatcaatt attttctgtt
     4981 cactttcaat aagaaaaaag tctttacttc catggat
//
LOCUS       DAYYKN010000168         4934 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000168 DAYYKN010000000
VERSION     DAYYKN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.170"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..1177)
                     /gene="xerS"
                     /locus_tag="ACHBB7_002640"
     CDS             complement(92..1177)
                     /gene="xerS"
                     /locus_tag="ACHBB7_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM9210528.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1526..1721
                     /locus_tag="ACHBB7_002641"
                     /pseudo
     CDS             1526..1721
                     /locus_tag="ACHBB7_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3253..3771)
                     /locus_tag="ACHBB7_002642"
     CDS             complement(3253..3771)
                     /locus_tag="ACHBB7_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM9210529.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4138..4386
                     /locus_tag="ACHBB7_002643"
     CDS             4138..4386
                     /locus_tag="ACHBB7_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210530.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1633 a          804 c          754 g         1743 t
ORIGIN      
        1 atataaaaat gataatgaag cttgccttta aagttgggct tttatctaat tttagagaca
       61 ggcttcatta ttatactatt ttttactaca tttacaaagc gttcaaagca tcaattgtag
      121 ctgcattgtc tatatgagta tataaagttg ttgtatttgt acttgtatgt cccaattgtt
      181 gggcagttaa taccaatgaa tttgtctgtt gatagagtct agtagccaaa gtatgacgta
      241 atttatgtgg actgacttgg actctgaatc cttttgaata ttttgctacc atccgttcga
      301 ttgcaatacc ggatatacgc ttggcttctc cttgataaat tgctagaaat aaagcttttt
      361 cgttttctgt tactttgtat cgttctttcc gaatagctag ataattagct aaatattcca
      421 ttgcaaatgc tgctatattt actgcgtctc tcttatttcc ttttctagtt actaccacat
      481 tattcttttc taaagatagg tcctctaaat taatatttac aacctcggag agtcttaatc
      541 cagatcctaa aatcaatgca ttgatcgcca ggtctctttc tacatctcgt tgataataga
      601 tcaaagctcg tttgctcaaa gtttttttat attcattggc aatataatct agataatttt
      661 gtgtatcttc gtctagaaat aattttttct cgattgcgtt tgcacgggca gctaacgtag
      721 ttttatctgt gatagttgct acttgatcca taggattatt ttctaagtat ggttttccta
      781 aaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgct gtgatatttc
      841 tattgatagt agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg
      901 tctctttttt tacattttcc tttgtgcgac gtttcaaatg agatttgtac aagtttagat
      961 cataggcagt taactgtctt atcgaggtga ttgaagtatc gggaacgata gactcactca
     1021 gaagccattc ataaaagagc ttataatttt ttagatattc taaagtagtt cgaggtgatt
     1081 gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga
     1141 ttgtatctat tttttgatat atttctgtat ttttcatgtt attcatcctt tcgataagta
     1201 ggggagagga gtatctctat aattggacta taccaattat agagtgtttt gtcaattggt
     1261 atactctgaa atttattaat ttatttcaaa aaagcagata aataggtata gatttaataa
     1321 tttaaaataa atggtataca tgaataggaa aggaaacaga aggaggcaat cttaattttt
     1381 taaattgtct aaattatata gaaagaacaa tctttctata taattagaca taagagagac
     1441 tacggttgat ttgatattaa caagcgtata tgaatgtata tacaaataaa aaaggggagg
     1501 gagaagttaa agaaaattca gagaagtggg gcactcagta gtattaacaa tttaaaagga
     1561 atttcttaaa gaaaatgggt tgcatcctgg agatacaatt tttttagata aagaaaaaat
     1621 tcaagcaaca attcaaaaaa atcttaactg ctgagattga tgtagcaata tctcaatcat
     1681 caacaaagta cgatcctgcc tttcaaaagc ttattgataa atgtttattc attataaaaa
     1741 taaaagaaaa atttatgtca gcttttttca tcaaagtggt aatgataatt cgtctattaa
     1801 ttgtattttt taggaaatat ggtataattg ttttaacgaa aaggtaattc atgatacacc
     1861 aaaaaccact agctggcggg ctagtggttt tcttttacgt tctattcaga ttaacgggta
     1921 ggcttactgt cttttttggc aataattttg caatcaagcc atttacagat gtaatgcgcg
     1981 gatacttctg ctaagattgc aaaaaacata tcgaaaaggt aattcataat acaacctcct
     2041 ccccttgcca taataggccg gggtagcaga cagcataaat attataccat aactgaaaat
     2101 ttcattttag tacatttcaa tatattgatg aaccagcaca ataaaacatg ccatgactaa
     2161 cagaaaaaaa cttattaaac cagatataac ttgtaaacat ccaactatat agaaaacgta
     2221 aattttctat atagttggat taagataacg actatttatt ttatcatatt tttactatac
     2281 cctatacttt tacttggtat atttatgaat aatcgcaaat ttacccctat actactagtt
     2341 tgtaagttag attaataaaa atactaaagt tatttgttct tttattaaat acacctacaa
     2401 cttataaatt gatggtattt agataacacg aaagaataaa aataacagct gatagtaaag
     2461 tttgttgacg attgctactc aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa
     2521 acttatgaaa tgaaagacct tttacatctt gcagaaatcg ttactgggat taaactaagc
     2581 aataaaaact aatgttcccc tcattctttg aacttctact atttgattta tttattctga
     2641 taaatgacga tttcaccttc aggatcttca aatcccgtat agaatttatt taagatctct
     2701 tcagtatcag taataggata ggcgataggc cacataccct ttgatggtta gaataaactg
     2761 ctcaaaatct gtatcgtatg ttggctttag catattaaca aaggctatct ccaaattata
     2821 aaccataagc gttgaatcgt tcttaacaat ttcaattctt cctaacccaa agtcagaaga
     2881 tataattacc ggacttgcct tacccttcat gtgggttgtt gagattccat atttaaaggg
     2941 aattgtcggt actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg
     3001 ctagtctcct aagtcccttt gtagcggata tggcaccata atctcatttt atcgaagtga
     3061 gtcatggttt atctgttggc caaccaaaat tgatacaatc taacgatgca ccctctacgg
     3121 cattctaggg gagtgcactt gcattcaccc aataaaaacc ggcagttagc aaagaactta
     3181 tgtagtcgta tgttcttact gttaacatac aagaggtctc ctttccacaa ttgcttagca
     3241 gtaaacggga atttacaaag ctaatctctt ccattttctt atctttgttc taaaagtccc
     3301 aaatggagca actgtattta catgaataaa tttatatact tcccatacag ccgttttggt
     3361 tgcctcgtct gcccattttc ttttgtgtgc agtgaataga tcttcatcac tcattgaatc
     3421 aatcatcatg ttaatatcat caatattacg attgagaatt tcctctaatt ctttcaatgg
     3481 tagatgtgca taggtatttg taaaccactt atataactct cctaactgat tccatttaaa
     3541 ttgatcagaa ggcgttttaa cagataatcc atttctttca tcattttccc attttagaat
     3601 taaattcgtc caaccaactt gatatgctaa attttcagca ggtgttctat ccacttcctc
     3661 aactctttta tccttcaatt cttcaggaat agtttcaaat tctaaaatat atttagtata
     3721 agtcctattt atttcttttt ttaattcttc tttattttta tatgttctca ttatattacc
     3781 tccataaatt cctaattgtc tcctttaatt catctataat ttcatccacg atattttaat
     3841 ccgttctaca atgcatatct gatatgcatt gactgtctaa ctcatatgtg aatgatttat
     3901 aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac
     3961 gaatatattt cttttgtagt aaaatcggac tggtttgcat atcattttct tcgtaaatat
     4021 caaaatcatg ttaactacta tcaattacta ataaaaacta ccactttaag cgttaagaac
     4081 tgttaagttt agttaagtga ggatagttca caaaatccat tgttaaactt cacgaaagtg
     4141 gtaaaactca aaatgcttta tataggatta cagcatcgct ctttcttcga ttgcgcgttg
     4201 ggtaaaaaat attatgaagt aaaaattgac gatgacacgt tccttacggc tcatcagatg
     4261 aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt taattctaaa aaagcgattg
     4321 ccatattcac gcctcgctcg acgaactatt aaaggtcaag atttacctga agatcatcct
     4381 ttttaggaaa ttgaagcttt ttatgttgca caatgcattt ataatactac aatgatgttt
     4441 tcaccagata ttcttctagg gtgcggtgta atgaaacaga tacacttgca aaagaaaatt
     4501 caccgcgaat ttactaaaat tatgaataat tatgttaatc ttccgaaatt tttttccaaa
     4561 attaagggat caagctggtg ttatagattg tttaaggttg gcaaaaaaat aacttctttt
     4621 taaacaatta tagaaaaccg atactctgtt aagttggttg acacggtgtc gatttttatg
     4681 actttcttat tctgaaactt cactaacgga cagtacctaa aaagtctata gtatggacgt
     4741 ttacgagtac tattttatca aaaatgctgc tcatttcaac ttatatgttt tagtgaacta
     4801 cccccactta gcttggatag gctacaatta actagacaga aaaattaagg tgtgtagact
     4861 agaagaaaac ataccaggag gaatttttat gtctaagaga acacgaagaa ctttttcaca
     4921 agaattcaag caac
//
LOCUS       DAYYKN010000169         4890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000169 DAYYKN010000000
VERSION     DAYYKN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.171"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2269)
                     /gene="esp"
                     /locus_tag="ACHBB7_002644"
     CDS             complement(<1..2269)
                     /gene="esp"
                     /locus_tag="ACHBB7_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM9210531.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
     gene            complement(2886..3239)
                     /locus_tag="ACHBB7_002645"
     CDS             complement(2886..3239)
                     /locus_tag="ACHBB7_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210532.1"
                     /translation="MSCFVESERLSNGNNSVLNEYDKVVINKVKSLCEEARESISHIN
                     GSNFKQTLFNLIKLDAKISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKL
                     NNYKLIDYKYLSESN"
     gene            complement(3336..4541)
                     /locus_tag="ACHBB7_002646"
     CDS             complement(3336..4541)
                     /locus_tag="ACHBB7_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM9210533.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
BASE COUNT         1550 a          899 c          641 g         1800 t
ORIGIN      
        1 aaatatcttt acttacagtt actgctaaat cggtcgtgcc tacagaacca tcttggtaaa
       61 caattttaat aggaattgtt ccagtaatcg gctccgattc tcctacacct ttagctggag
      121 tgaatgtaac aataccagta tttgcatcaa cacttgcatt accgaagtcc ttattataga
      181 catctaaaat tgaaaaggca tctttagcta aaggaacatc tggtttatca aatgtaggtt
      241 ttccactttg aactgtcgtt tctggagttg ttgttatagg tacgtatgtt gcatcatttt
      301 ccatagcttg agaagctttt acagcaaact ctacatccac gtcgaaagtt cgatttccga
      361 tcttaacagt tacaatacct ttcgtactat cagtaacgtt agaagtatca ggctctgttt
      421 tccatgaata ctctgcatct tcaggtaagt ttttattatt ccgaattgat tctttagcat
      481 ctggtttaaa tccaactacc acggtttgtt tatcaaccgc ttttggtgat tccttaataa
      541 cggttgaacc ttcttctggt ttatcaaaac ctggagaaac gatttggatt tttaattcat
      601 ctttcgcgat taatttactt gaatctacac ccgtaaattc aaattctaca gtatctgaaa
      661 ttggagcccc atctttttca tttggagcga tagttttttc tgctaataca gtaccttctt
      721 tatcaacaag ttgggctttg tgacctgttc ccgctaactc gtggatgaat actttcccct
      781 taactgttgt gtcaacatca tatacttctt ctaattcagg tttcccgata tatggattcg
      841 tgtctccgct ctcttcttta tcgtcagggt ttccatcacc atcaatatct tgcaaataat
      901 aataacctgt tccataagtg ttctggatta attttttatt cgtatccgac aaccaagaat
      961 caaagatgaa atcttcacca aatggtttat tttcttcaac ttgtgctgtt gctttcatat
     1021 cttctaagat ttcatctaat ggcttagtca attgtaaaac atatcgaata acgactggac
     1081 gtggacttgc attagcaaaa tcaataagtt tggtaagatc attatcagta attgaaccta
     1141 caccataatt ttcatgatct ttgcgccgtt caattgctaa atcagccact tttttccctg
     1201 attcaaaaga aacatcatat gttactttgt cagcctgaac catgtgcatc tgtatacccg
     1261 ctacatatgg aactaatcgt tcatcaattt tgtaattagc ataccaaggc gtgccctttg
     1321 cggaagctga atatgagaaa ttataatttt tcgttaactt atgatctaag attatagctc
     1381 cattagcacc tgtggatcct gaaccatctt ttgtttgagt attaggttcc tcatattttg
     1441 cacttactga tccatgttta aactggtcag gattattttc agatacatct aagctattat
     1501 agaaatcatc tgaatctgtt aaaaagtagc ccgtactatc aatacctggt acaattttat
     1561 catcgtcttg atgacttacc aagtagattc gatacattaa acgatcggtc ttgagattgt
     1621 ctatgcctat ctctttaaaa atttcatcta agctcttttc aaacgtaatt accccattag
     1681 gagcagtttg tgtatcagtt gtttcagccc cagcaaataa tccatcactt gcacgaatat
     1741 aattgacctt ccaaatattt gtaggtctac ccagcgaatc attaattctt acaaaggtcc
     1801 gtctagaaga tccaattcct ttctttggat cgacctgaat tttttctaca tatttagcta
     1861 atcgttcatc taattgaaga cggatttccc agtttttagt tgatgctgca atattatgtt
     1921 ttccgtaaac ttcaaatcca attgtattgc cttttagtgt gtctccagtt agttcttgag
     1981 gactaaattg taaatccgaa aagttataat ttttcgtata accaggattc tcctgaaaag
     2041 tattccccga agctactttg tcacctactt gtacggagct tatcgctgtt tcaagtgggg
     2101 tgcttggaga aatttcatct tgtgcttgaa ctagttcagt tgttcccaag acaagaccaa
     2161 caccaaccaa aacagatgca accccaacac ttaatttttt gatactgtac ttcaatactc
     2221 gtttctttgt tttttctaga aatactctct tattattctt gctaaccatt attcaaatca
     2281 actcccttaa tagttatttt cttagtaaga ataaaagata aatctgatac gtattattca
     2341 cagaatagga atcttactaa acttatgtta actagtttac ttaaagaatt tcatgcacat
     2401 tacaacaaaa aaagtacatt tcttcatgtt tttttcggat atttgtgctt tttaaccatt
     2461 cagcagctga aaacatataa aaagatatgt atgtattctt tctgaaatta agttcatttc
     2521 tatgcgatga agcatgtttt caaaatatta ctctttctta gattaaactt cagagtattc
     2581 aaatagcgga ataaggagcc ttatcccgca ttactgaatg agactgtgac ataagtaaaa
     2641 aggtgacttc aaattccttc ttcggaattt gaaatcacct tctttataat tataaagtat
     2701 cggtttcaca gcattcctga atgattccta gaatcttaaa cgaatgattg gagttatcaa
     2761 cattttttca ctcgttttta gcgaaacaaa aaatgacatt tgtcacagcc tcattaaatt
     2821 attgattctt tttctttata attgaattgt ccatatttaa agtgtctagt actctacatg
     2881 aataattaat tgctttcact caaatactta taatctatta acttgtagtt gtttaacttt
     2941 tctgaaaaag ttagacccac gtaataatct tcccagcttt ctctttcaat taactgatcc
     3001 aatttttgat aatccataaa ttcatcatgt attaaaaaga ataaataact tgatatttta
     3061 gcatctagct taatcagatt aaataatgtt tgcttaaaat tgctaccatt aatatgactg
     3121 atagattctc tagcttcttc acataaactt tttactttat taattactac tttgtcatat
     3181 tcatttaaca cactattatt accattactt agacgctcag attctacgaa acaactcaaa
     3241 tcagaaattt cccaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3301 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattatcc
     3361 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3421 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3481 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3541 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3601 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3661 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3721 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3781 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3841 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3901 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     3961 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4021 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagatttcct
     4081 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4141 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4201 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4261 aaatctttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4321 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4381 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4441 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4501 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4561 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4621 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4681 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
     4741 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4801 aggaattttt cgtttctaaa tactccaatt tgggaatcca ataaaaattt tcgttcaaat
     4861 acgtgtctat tcgaactctt ttctagttgt
//
LOCUS       DAYYKN010000170         4864 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000170 DAYYKN010000000
VERSION     DAYYKN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.172"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..939
                     /locus_tag="ACHBB7_002647"
     CDS             139..939
                     /locus_tag="ACHBB7_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9210534.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            958..2826
                     /locus_tag="ACHBB7_002648"
     CDS             958..2826
                     /locus_tag="ACHBB7_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM9210535.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            2819..3310
                     /locus_tag="ACHBB7_002649"
     CDS             2819..3310
                     /locus_tag="ACHBB7_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM9210536.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3298..3852
                     /locus_tag="ACHBB7_002650"
     CDS             3298..3852
                     /locus_tag="ACHBB7_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM9210537.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            3870..>4864
                     /locus_tag="ACHBB7_002651"
     CDS             3870..>4864
                     /locus_tag="ACHBB7_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM9210538.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1691 a          761 c         1026 g         1386 t
ORIGIN      
        1 ctatgcaaaa agatgaaaat tttttttgct cttccattag tacacatttt ttagctgaag
       61 ctgtaaattt catgtcttaa ttttttttag tgagctttta tactggattt gtaagaaaaa
      121 agaaggagag gatcacatat gtcggattgg ttggatattg caggaaaagt agtaattgta
      181 actggaggtt cctcaggaat tggaagaagt atcgtcgaaa atttgttgaa gcagaatgca
      241 caagtagcga attttgatgt aactgaatgc cgtatacagc atgaaaacct actatcttta
      301 aaagtcgatg tgtcttctaa aacggacata gaagaaggga tatataaagt aatgaaacat
      361 ttcaagacga tcgatggtct agtcaataat gcaggaataa atattccttc tcttctaata
      421 gatagaaatc atccaaaaag caagtatgaa ttatcagaac aagtttttga taagatgatc
      481 gctgtcaatc aaaaaagtgt atatttgatg agccaagcag ttggaagaat cttggtgcaa
      541 aagggatcag gagtgatcgt gaatctttct tctgaatcag gattggaagg atcagaagga
      601 cagagctgtt atgctgctac aaaagcagcg atgaacagtt ttactcgttc ttgggcaaaa
      661 gagttaggaa agaggaatgt tcgtgtggta ggtgttgcac cagggatact ggaagaaacc
      721 ggtttgagaa cacaagaata tgaagaagct ctttcttata cgagaggtat aagcgtcgaa
      781 cagctgagaa acggttacag tcgaacaagt acgatccctc taggaaggag tggtaagctg
      841 caagaagtgg cggatctagt ctgctattat ctttcagaaa gatcaagtta tatcactgga
      901 gtcacgacta atatttcagg aggtaaaaca agaggataaa ggataggggg gacgcacgtg
      961 acgctagtaa acagatggta ccaattattg aaattactag tcactcacaa gaaagtttca
     1021 atccaggaag tgcaaaaaga aatcaaaacc agtaatcaaa ccataaaaaa agatattggg
     1081 gagttgaatg aagaaattgc cggtattgca aaaatcatcc aaaaaaacaa acattttgaa
     1141 cttgaaattt atgattttga tttttttgat gagatcatgc aaggaaaatt gaaaacagcc
     1201 agtgatttca attcggcatc caaaagaatc gcttatttac taaataggct gatcgaaaca
     1261 gaggatttct tacgtatgga tgatttgtct gaagaagtcg gtgtcagcag aggaacagtg
     1321 gtcaaggatc taaagacatt aaaagagatg atcaatgatt ttgatgtaaa gattacaggc
     1381 acaccaaata aaggactaag aataatagga gatgagctag aattacgact acttttattt
     1441 tattttgttt ctccctactt ttcagagacg aaaacagaac agtttctaga agaaaaacaa
     1501 gcaatcaaac attttcagaa gaagacgaat gctagtaaac aagaaatcac ccttttaacg
     1561 aaagttatcg gtatttcttt gaatagaatc tctagtaact atctgttgaa gaagcccatc
     1621 aatcgttaca gtgggtgttt tgaatatatc caagaattca atgaactgat caatcagttg
     1681 gaagaaatct atgaactaac tttgagccaa tatgaaaaag attttatcag ttttcctttg
     1741 aacatcatca acacaagtat aacgatgata aggaaagcta actctgattt gcgcttatat
     1801 ttcgacccca tgatgatgcg tattcgacga ttagatctca ttgatctgga tgagtccttc
     1861 ttatatcaag agatgaaaca gcacctctct ttattgataa atcgggtaat ttttcgttat
     1921 cgattgactg atttatttta tggtgagata gaaaaaaagt atcctttttc ttaccaaatc
     1981 gctagcgaat gcatacaagc attagaagct atcttgcaac ataagatctc taaagtagag
     2041 atcagctacc taacgttgta tttggagtta gcatttcgat atcaaaaaga gcatttttcc
     2101 tctaaaaaag tagccattgt tcatacgaca ggaaaaggaa cagcattaat gatccaaaga
     2161 caaatcaaaa ggatattggg gagggatata caaataacac atgtttcaga agaacaatat
     2221 gaaaaagtag atctaaacaa atatttcgct gtttttacaa cgattccgtt aaaaaatgta
     2281 aatgaagaga caccggtgat ccatttgact aatctttttg atgactcttg gttaagggca
     2341 caatggcaaa aagcaaaaaa aaagcgatta gccgggtttc attctactgt ttttttctat
     2401 caaatatgga aagaaaaaaa gacgtatcaa gaatatttaa acaaattagt cgctcctttg
     2461 atcagaggct attatgtgga tgaagaattt gaaaaaagga tcttgaaaag agaatatcag
     2521 caaagcacat ttgttaacaa agaatttgct tttcctcaca caaccaacca gctatctcaa
     2581 cacattgttt tatcagtagg gctaataact gatcaaggtt tagcagaaac aggaggaaat
     2641 ggagttattt ttttatttgc catacctgaa gaaatgacgg ctgaagttga gaatgaatta
     2701 tttgagctat acgatatcct atttgaactc ataaaaatgg gaatagatga gtttattgaa
     2761 ttagcaccga cacaagaatc atttttaaaa ctaatcaaaa gtaaaggaga gagacttcat
     2821 gaatgatatg tatctattca tagttttcgc agcttcagct tatgtgctgc aagcagttat
     2881 cagttggaag caactaaagc atttcaatca aacatatagc gaactgcgaa gaaaaggaaa
     2941 agtagcgatt ggtaaacgac ctgggaaaat ccgttcaggc acgattgtta tgctagctat
     3001 agacgatcat gatcaagttt tagaagtcag aaaaatgcaa ggggttacat ctttggcaaa
     3061 atttcattca ttacctgtct atgcgggtga agatatccat tacatcgatc gttaccatcc
     3121 ctcagttcga gcggaagatc gtttgacaca agcagcttta gaaaatgcca gagaagtata
     3181 cttacgaata gaggcaggaa atgaaaaaag tgaacaacct tcattgcctt ttattgctat
     3241 gctcaataat tttcggatat ggaaaagtac gatcaaagaa aaaaggagtg acaaacgatg
     3301 gatatcataa caaaatttgc tgagggattt atgaggctgt tccaaacagg tgcagaaaca
     3361 ttcataagct ggatgacagg aatcgtacca gttgtcttga tgctgatggt tgcaatgaat
     3421 acgctgattg cttttttagg tgaagaccga gtaacgaaag tagccaaatt atcagcgaaa
     3481 aatccgctta ctcgttacct tgtgttaccg ttcttatctg cttttatgtt gggaaatcct
     3541 atgtcattta caatggctcg tttccttcca gaatactaca aaccaagcta ttatgctgct
     3601 caagctcaat tttgtcatac gagcaatggt gtattccccc atattaatcc tggagaattg
     3661 tttgtatgga tggggattgc tcaagggatc gaaaccttag ggctaaattc gatggagctg
     3721 gctatccgat acttgcttgt gggactgctg atgaacttta ttggtggttg gataactgat
     3781 tttactactg gatttgtatg cagacagcaa ggaatcgttc ttagcaaaaa agtagagctt
     3841 tcagttgatt agagaaagga gagttttaaa tgtatggtga tattaagatc gtaagaggaa
     3901 acggcgggta tggaggtcct ttgattatta ggccgacaaa agataaacac aagttcatct
     3961 atgtcacagg tggaggtgag aggccaatga tcgtagataa aatagaagaa ctgacaggta
     4021 tggagtctat caatgggttc aaaacatcaa tacctgatag tgaaatcgcg cttgcaatcg
     4081 ttgactgtgg agggactctt agatgtggaa tttatcctaa aaaagggata ccaaccatca
     4141 atatcgtttc cacaggaaaa agtggcccat tagcacaata tatcactgaa gatatttatg
     4201 tttcagcagt agggttagaa caaatacagc cactaatgga aactgatgat aacaaagaaa
     4261 cagtaaatac tgcatctcca gataaaaagt acaaatatga tacttctaaa aagataaccg
     4321 agcaaaaagc agagcagcaa aactggatag caaaaattgg aatgggcgcg ggaaaagtag
     4381 ttgctacatt caatcaatca gcaagagaag cagtagatac gatgatccgg acagtgatcc
     4441 cttttatggc tttcgtttcc cttttgattg gtgtgataca aggttctggg atcggtaatg
     4501 tatttgctaa cattatggca cctttagcag gaaatgcagt tggattaaca atcattggct
     4561 ttatttgttc actgccattt ttatctcctt tgcttggtcc aggggcagtt attagccaag
     4621 tgattggaac attgatcggt gtagagattg ggaaaggaaa tattcctccc caattagcat
     4681 taccggcact atttgctatc aatactcaaa atgcatgtga ttttattcca gtcggtctag
     4741 gtttggaaga agcaaaagct gagacagtag aagtaggtgt accatcagtt ctttattcaa
     4801 gatttcttaa cggcatccca cgagttatca ttgcttgggt agcaagtttt ggattatata
     4861 gctg
//
LOCUS       DAYYKN010000171         4855 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000171 DAYYKN010000000
VERSION     DAYYKN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.173"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(366..1157)
                     /gene="msrA"
                     /locus_tag="ACHBB7_002652"
     CDS             complement(366..1157)
                     /gene="msrA"
                     /locus_tag="ACHBB7_002652"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9210539.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1348..1983)
                     /locus_tag="ACHBB7_002653"
     CDS             complement(1348..1983)
                     /locus_tag="ACHBB7_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM9210540.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2249..3301)
                     /locus_tag="ACHBB7_002654"
     CDS             complement(2249..3301)
                     /locus_tag="ACHBB7_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9210541.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3314..4252)
                     /locus_tag="ACHBB7_002655"
     CDS             complement(3314..4252)
                     /locus_tag="ACHBB7_002655"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9210542.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         1577 a         1020 c          737 g         1521 t
ORIGIN      
        1 atagttattg tttgcgcttt tatacggaat ttagttcatc attaatgtgg aattcgatta
       61 ggtgtctatt aattatcaag atgattctct acaaaattcc atagaaaaat caagattcta
      121 caattcgaat tctctttcac atggaaataa aggctagtaa aaaaacagga gttgtgacaa
      181 aagacttgtc atacctcctg tttccttgcg acttgaagct gaatgctttg cgccacaacc
      241 ttttcctcgg tgttctcaaa gcaactcctc gaaataattc ataactgtcc aaaaatatga
      301 aaagctattt ttgtcagttg ctcattattt tgtcggagct gaacgctttg cgccacaacc
      361 ttcctctatc tatgtcgctg aaaaaataaa tgaagctgat gcgaatgctt gatcttttta
      421 tatcttttag gttgcttctg ataaaattgt tgatgatgat tttcagctgg ccaaaaagtc
      481 tttgcttggc gaatctctgt aacgatagga cggctaaatc ggttagattg aatcagttgt
      541 tctcttgatt tttcagccaa ttgcctttgc ttttcattaa caacaaatag aattgaacga
      601 tattgtttcc cccgatcttg aaattgacca aaggcatctg tagggtctga aatttgccaa
      661 taaatctcca acagctcttc ataggttatt tgacgtgtat caaatatgat ctcaacagct
      721 tcaacatgcc cagtatatcc accactgact tgatcgtaat ttggtttttt ggtctctcca
      781 cctgtataac cagataacac tgagattaca cctggttttg tttcaaaagg ctcaaccata
      841 caccagaaac agccaccggc aaaaatcgcc cgttcttggt ataagggtga agttatctta
      901 tgtttggcat aatcataagc ctttgcactc gctgactccc ctgtgattct taaataaaaa
      961 tccatcgcat ctggtgtcaa attatctcgt agtgctagcg gacgcaagtt gacctctagt
     1021 ttttctatca cctgctttat cgacattttt gctaggtctt cttttgcctc ttcaaattgt
     1081 gttctttccc attctcttgt atttcgattt aaaataatat tataaaggtc tatttccatc
     1141 tgctccacat ctaacatgaa gtcaccctct ttcaagtttt cttcttattc attcaaaaaa
     1201 tgactacctg aaatttagca caacgcataa caagttaaga ctaacttgct tttcgctctc
     1261 ctcatctcta ttcttagtcg aacacatcca cacgaatggt tacccataga gaaattcttt
     1321 tacttaatca gcttcacact gctttttcta ctttcgtagt tgaaaaaaac ttaaacaaat
     1381 catcggcaaa tagttaaaga aaaaacttaa gcgttgaaag aagcccaatt gcttaaagaa
     1441 ggcaaactga ctaatcgacg gaatccgtgc taatccgtaa agacctgcaa ttagtaaact
     1501 aataactgtt aaaacaagat aaaaatggct aagcgtacct tgaccacttt gacgataaag
     1561 cagaacaagt aacaatggga aaagtagaaa gcctgcatag cctagcccag aacctatatt
     1621 atggatccaa gtcgatagat tccaagagct ttcattcgta ttgatactga ataacccagt
     1681 aaaaatacaa tcacctatcc catataaacc aactgttgca acagcaagta ttgcaagtgt
     1741 ttttgatgtt ccaataaata ggtgatacaa cgcaggcaac gaaagaacaa aaaaaagtcc
     1801 agatacaact gaccagacca agaaagcacg tctgactgga ctatctacgt caccaaacac
     1861 actaattacg gtagtcattt gattcagttt gggataaaaa aggccaagga tatacggtgt
     1921 cgataaatca ctaatcacac ctaataataa aaaataaaaa ccatattttt gcaaaaaaat
     1981 cattttcttc tcctactttt tagtgattat tcgatacatc caacaatcaa attttttctc
     2041 attttaacac atatttttat tctagttgcc ttttgaacaa tcaataacat aaaggtgagt
     2101 ttcttctaca ttttaaaaaa tcgaatggca ctgaagagtt cagttgtcag catcaatctc
     2161 ctactcaaaa aggcaaaaaa acgtaagtca gatgttgttg acatctcact tacgcatcca
     2221 ttgattgaaa atttcttagt aaaatctact agcctaaaaa tttgaagact tcttctcgat
     2281 catacagttt aaatactttc tcgcataaaa attgagtaat tagtgccgca aaaacaggga
     2341 tgactaacca acaaagaaca acaagaaagc catttagccc tgcatctaaa gaagccaacg
     2401 gaccaacaag tccgaccacg ccgaaaccag cagaagcagg cgtacctgaa atagaaaata
     2461 gagcgacagg aatagctgta atcactgcac taactaaaca aggaataaga atcacaggtt
     2521 ttctaaatag attaggcatc atcatcttca tagcgcctaa agcaatcgca attgtcacac
     2581 ctgacttatt aaccttccaa gagttcacta ctaaaacaaa agtggttgca gctacgccca
     2641 tcgcagccgc accagcagaa acaccatcta gttggattgc cattccgatt gctactgttg
     2701 aaataggaga gataatcaaa aaggcaaagg aacaaccaat caagatagac attaacagcg
     2761 gttgtaaggt agtgaagtta ttaatcaatg atccaattgc agaagtaatt gctgttacaa
     2821 aagggaataa ataaaaacca agtaaacctg cacctacacc tacaacaatc ggtgttaaca
     2881 caatcgacac tgacccaaaa ctatttccaa tcagtaaaat cagcccgaca gcaatcgatg
     2941 ctgtcagcat cgtattaata atatcgcctg taccagcacc aacgtaaaca cccgtttcac
     3001 ccaaatcagg tgtgaatttg actacaccag aaccaacaaa agaagctccg gctacaacca
     3061 tcattctcat cggatcaaat ccaaattgta aggcaatcaa accaccaatg atcaaaggtg
     3121 ttgctagttg aaaaataact gctaactgtg cgatcgttac cgcaaaggga tacgcactaa
     3181 aatattttaa aatggcacct aatactgcat taggaatcaa tccaataatg attcctgttg
     3241 ctgtaccagc gaggacttta ttcagaaaaa ttttcggtga tattttttgt gaagtttcca
     3301 ttcaatctct cctttatttt acgttcaaac tatcttcttt tgcatggaca agctcagtca
     3361 agaaatcaca ataaggcgtc gcaacaccat atttcttccc ttttctagaa attgcgccat
     3421 tgatataatc gatttctgtt aatcgatggt tcttaatcaa gtcttgatac attgaaggat
     3481 aatgtaaccc aattgtttcc gggtcaaaac aagattcaca atgtgcaatg acttctggga
     3541 catctagttc aatcttctct actgctgcta ctttggcaaa ctcattgaca atcgtcgcca
     3601 ccattttatg agcagtcgat gtttttccta gctcagccat attaacgtct aagattgtgc
     3661 atagtccgtt cattgttccg ttcacacatg ctttgcgata aatagaatag tgaatattgt
     3721 cagaaaaatg agcatttaat cctgattcag acaatttgtc tgctaatttt tttgcagctg
     3781 cttcttttcc atcacctaaa ttttgtaatt ctactgaacc acttccaaat aatttgacct
     3841 gaccaggacc ttcaagacct gccgtccaca tggtatttcc aatatagata ttttccattg
     3901 gtacaaattt ctcaataata tcttcatgcc cgataccatt taatagacat aagacctctg
     3961 tatctttttt gattaatgat tggatatcct gcagcatttt ttccagctgc atcgctttgg
     4021 taaatagaat aatcagatca acttgatctt ctttttctac ttcggattga agaacgattg
     4081 gtagttttgc ttctacctct ttaccattaa aatttgcttg taatccatgc tctttgattt
     4141 gttggacatg ttctgcccag ccatcgatta ataatacttc gttcccactt tggtgaagca
     4201 ttaacccaaa gcgacttccc attgcgccag cgcctgcaat tgctattttc attgattctt
     4261 cctccttttt ctttctatta tattcatttg agaatcattt cacaaccttg ttttgttatt
     4321 cttctcttat ttatcatgtt tatatccacc attcaagtta ttttatcagc aaatcaataa
     4381 aaactggaat agaatcccaa tttcatcgta agagaaacta gactagcaat aagaaaatag
     4441 aaacattcat agaaaaaacc tcactcctct gattataaaa gagggggcat taacctcgtg
     4501 tatactaaaa ccttccattc attcgatgga tgattttctt tttgtgaaaa aatattcatt
     4561 gtagtacgat gttgcgcaac acagacggat ggtattgctt attatttatt aaaatcgaac
     4621 aatacactta tttaaaaact tctttcccag attttcttcc atttttacag ttataactag
     4681 tattatcatg aaaaatagcc ttttattgtc tatgggaata ataatttaaa attggtactt
     4741 ttttattaat tagttcatcg cccaataatc tctccagttt cttagcatct cctgactcaa
     4801 ccggaattat ccaagcccag tccaaataat tttttaaaat ctcatcatat tttgc
//
LOCUS       DAYYKN010000172         4732 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000172 DAYYKN010000000
VERSION     DAYYKN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.174"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..828)
                     /locus_tag="ACHBB7_002656"
     CDS             complement(55..828)
                     /locus_tag="ACHBB7_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9210543.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            972..1322
                     /locus_tag="ACHBB7_002657"
     CDS             972..1322
                     /locus_tag="ACHBB7_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM9210544.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1303..2019
                     /locus_tag="ACHBB7_002658"
     CDS             1303..2019
                     /locus_tag="ACHBB7_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM9210545.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2019..3467
                     /locus_tag="ACHBB7_002659"
     CDS             2019..3467
                     /locus_tag="ACHBB7_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9210546.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3537..4046)
                     /locus_tag="ACHBB7_002660"
     CDS             complement(3537..4046)
                     /locus_tag="ACHBB7_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210547.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4036..>4732)
                     /locus_tag="ACHBB7_002661"
     CDS             complement(4036..>4732)
                     /locus_tag="ACHBB7_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210548.1"
                     /translation="LIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSALANQVQDRLS
                     FIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNKQELINWRLTG
                     LSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVLKTTELPDIRD
                     ILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLILSLFPFTFFI
                     HHLQSRRPGRNET"
BASE COUNT         1617 a          811 c          958 g         1346 t
ORIGIN      
        1 ttaagaaaaa ataagcgatt ttcttaagaa aagaggtagc ttcaaaaaaa gatcttaaat
       61 cagatcaaaa tacttcaatg cgtggacaat tccgccttct gtatttttct ttgtgataaa
      121 ggtagaaagt tcttttaatc cgtcataggc gttgtccata gcgattttgt tgtcgcaagc
      181 ttccaacaag gcaaaatcat tcggaccatc gccaaatcca aaagtaggaa ctttttccag
      241 ctgaagttct ttttgcagac gtttgacacc tgcccctttt gagattcctt tcttcactgt
      301 atcgatcgag taaggaccat tccgataaaa agttagctca ggaaaatgtt cgtgatagta
      361 ttcgtcacct gattgagata agatcagcaa catatttact gtgttttctt taaagcctaa
      421 aggatcaatt tccggaacat ctgaatgaat gaagtcgtaa gcattgatca ttgtttggct
      481 atgaccggta caccagattt tctgatcgtt atagtaagaa agttcatgac cgttcaactg
      541 tacatgatca tacatttttt gacattcttc tgaagaaaat gtttgagaat agatttcttc
      601 gccgtctact cgaatgaaag aaccattcat ggtgaccgca gaagtaatgc ccgcatcttc
      661 catgatagag tggatttctg cttctgtacg tcctgtagcg atgacgggga gaacattgtt
      721 ttcttttaat gcgttcatag cttttgccac ttcgggtgtg atttttgatt tcgcatctag
      781 taatgttcca tcaagatcaa agaaagtgat tgcacgataa tttgacacaa ataaaactcc
      841 ttttttattg gaataagttt atcttaacaa agaatgaaca aaagacaaac ctttcgtgaa
      901 gaattcctga acatattggt tcagtcaaca atttagagta tactataaga agagaattta
      961 ggagggaaag aatgattttt ttcagtattg gtatcttatt cttaataatt ggcagtcttt
     1021 ttcggattct gccctcaaaa ggaaatctgc ctttttatgg gtatcattcg cctcttgccg
     1081 caaaaacaga cgctcattgg cggctggctc aaaagacaag cggaaactgg ttttttctta
     1141 tgggcttact gatggcattg atcggctatt atttaaagac atctgggcat acgaattatt
     1201 ttctgattga aatgcttctg ctagtatttc caattatgcc tatttttatt atgacagaaa
     1261 agaaattaca gaaatatgac ctggaaactg gagggaacga taatgaatat tttaatgatt
     1321 gaagataatc aatctgtttc tgaaatgatg cagatgtttt tcttaaatga aggttgggaa
     1381 gcaactttca agtatgatgg aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat
     1441 tgggatatga ttacactaga cctgaatttg cccacactgg acggtatgct agtaggaaga
     1501 gagatccgca aagtttcaag tactgtgccg attatcatgc tgacagctag agattctgaa
     1561 agcgatcagg tgatcggctt agagatgggg gcagatgatt atgtcacaaa acctttcagt
     1621 cctctgacgc ttattgctcg aatgaaagct ttgcatcgcc gtagtgagtt ggcagaagca
     1681 agagaagaag cagcagtttc agatgatcat tttgatatcg agactagtca tttcaagatg
     1741 aatacgaaaa cacgagaagc gtatctggac aaccggccaa ttgaaggact gacgcctaaa
     1801 gagttcgatc tattgtttac tttagcaaaa aaaccacgtc aagtattctc tcgggagcag
     1861 ttgcttgaat tagtctggga ttatcagtac tttggtgatg aacggacagt ggacgcccat
     1921 atcaagaaat tacgacaaaa aatcgaaaag gtcggaccac aagtcatcca aactgtttgg
     1981 ggagtcggct acaaattcga tgattctggt gttgcctaat gcgttatttg tatcagcaac
     2041 tcttagcttt ttgcggaatg gtcgcgttga tcttattgat cgttggagtt tcttttacac
     2101 aactgacaaa acaaacgatt gaagaaaaca attaccagca actgtttgga tatgctgaat
     2161 cagttgaaaa gacaacgcaa acctatgccg atacgctccc gcagtttagt caagatcagg
     2221 cgtttcaaaa tgcattgctg ctgacagagc aagtactgac agaacaaaat gtgaacttta
     2281 tatttatcga taaaaacgaa cgtgtcattt atccgactaa tacagcttta ctgaatttta
     2341 ccttaacagc tgatcaatgg aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga
     2401 ataaaaatat tttaggacaa gcacaggcga cctcttacgc acttgtgcca tttaatttga
     2461 atcatgaatt ctatggagca cttgtagtta gtcaacctgc gcgaaatatc gataacagcg
     2521 ttcgtccaat tacactgaac ctgttcaaag gatttatttt ttccagtatc gttgcagtta
     2581 ttgccagcta tgcttttgca gcattccaag ttaagcgtat caatcggtta aggaatgcga
     2641 cgaaagaagt gacgaatggt aattttgatg tacagctccc cgtacatgac aaagatgaat
     2701 ttgatgagtt agcagatgat ttcaataaaa tgacgaattc attgaaagaa tctcaagcag
     2761 aaattgaaga acaagaaaat cgtcgaagac aatttatggc agatgcttct cacgagatga
     2821 gaacacctct tacgacaatc aacggacttc tggaagggtt agaatacaat gcaatccctg
     2881 aaaatcagcg agaaaacgcg attaaattga tgaaaaatga aacagagcgt ctgatccgtt
     2941 tggtgaatga aaacttggat tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta
     3001 aaaaattcaa tggaacagaa acattgaaaa atattgttgc acagttagaa gctaaagcag
     3061 aagcagcagg agatacattg acattgaaag ccgttgatga tattgatgtt tacgctgatt
     3121 atgaccgatt tgtccaaatc atggtcaata ttattcaaaa cgcgatacag tttactgaaa
     3181 acggccaaat cacgattact ttagaaaaag gctatcttga aacgatcatc acgattgaag
     3241 atacgggaat tggaatgtct gaacaacaaa tgaaaagtat ctgggatcgt tattataaag
     3301 tagatccttc aagaaaaaat acaaaatatg gtgaatcagg attaggttta ccaatcgtgc
     3361 agcaacttgt acgcttgcat aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa
     3421 caaaattcgc tgttcgtttg cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta
     3481 taaaagatca tgaatcgatc ttctataccg cttgttttta ctataaaatg attgatttag
     3541 cccgcgggct ctaaacgatc aatataagga ctttgagcaa tagtagcatc atgagtaaag
     3601 agtaagatac cttgattttc ttcatgagct aaataagaaa gcaagtggag tagctcttgg
     3661 atctccaata cactaagttc atcaaatgta tcttcaataa caatattgtt tcgcttggaa
     3721 agcaaggcat gaagtaattg cagctttact ttttcaaaag acgtcaactc agctgccgca
     3781 caattaagca aggaaggaga gatatcaagt ttttcttgat aggccaaacg atcttgtttg
     3841 atttcttttt ccggtacatc caacaggaca tggtctttaa gattcagata aggaaacatc
     3901 ggccaatttt tttggatgat cgcaaaatta tccagttttg caacaagaac ctcttttttc
     3961 aagtgctcca atattttatg ctgtttttcg agaggacaca aaataacggt caagtgttca
     4021 gcatgaatca aatattcagg tttcattacg tccaggcctc ctactttgca aatgatggat
     4081 aaaaaaagtg aaagggaata gactaagaat gagacagcat ccactgagcc caataagagt
     4141 ttgggcaaat cccttaagta tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa
     4201 aaaagtctgt tgatccatcg gaattgtaag tccgcttacc gaagtcaaga tatcacggat
     4261 atctggcagt tcagtagtct ttaagacaag aaaattgatc ctttgaagaa gcgcttcata
     4321 aactgactgg aagctgacga tccacaataa aaccaagata caacaaagaa agagaggaaa
     4381 gaacagctgc cagaaaataa aaaggaataa tttggtttta gaaagaccag tcaggcgcca
     4441 attgatcaat tcctgcttgt tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa
     4501 ggcaaataga aaaaataaac tgaatatgcc ttttatataa aatcggctag ctaattctaa
     4561 agccggaaga agctctgtag atgcagaagc gccaataaat gaaagccgat cttggacttg
     4621 attggctaaa gccgattgcg aatggtaaag agtcaggcat gctatcaata aaaaagcaga
     4681 aaagcttgaa aataaacaat agagtattgt ctgctttttg taataaatta at
//
LOCUS       DAYYKN010000173         4646 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000173 DAYYKN010000000
VERSION     DAYYKN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.175"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..865
                     /gene="vanY"
                     /locus_tag="ACHBB7_002662"
     CDS             59..865
                     /gene="vanY"
                     /locus_tag="ACHBB7_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFM9210549.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            883..1710
                     /gene="vanW-B"
                     /locus_tag="ACHBB7_002663"
     CDS             883..1710
                     /gene="vanW-B"
                     /locus_tag="ACHBB7_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFM9210550.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1707..2678
                     /locus_tag="ACHBB7_002664"
     CDS             1707..2678
                     /locus_tag="ACHBB7_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM9210551.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2671..3699
                     /gene="vanB"
                     /locus_tag="ACHBB7_002665"
     CDS             2671..3699
                     /gene="vanB"
                     /locus_tag="ACHBB7_002665"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM9210552.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3705..4313
                     /locus_tag="ACHBB7_002666"
     CDS             3705..4313
                     /locus_tag="ACHBB7_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM9210553.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4379..4552)
                     /locus_tag="ACHBB7_002667"
     CDS             complement(4379..4552)
                     /locus_tag="ACHBB7_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210554.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1305 a          984 c         1240 g         1117 t
ORIGIN      
        1 aataacaatc gtaccagcca caaatcgtag ttttattgca aaggaggcat tcgatcaaat
       61 ggaaaaaagc aactatcatt ccaatgcgga tcatcacaaa cggcatatga aacaatccgt
      121 ggaaaagcgg gcttttctat gtgcgttcat tatttcgttc acagtctgca cgctgttttt
      181 ggggcggaga ctggcttccg tattggaggc aacacagata ccgcccatcc ctgcaactca
      241 tacaggcagc agcactgacg tagtggagaa tttggaggaa aacgctcttg ccaccgccaa
      301 agaacaggga gatgaacagg aatggagcct gattttagtg aacaggcaga accccatccc
      361 cgcacagtac gatgtggagc ttgagcaact atcaaatggt gagcggatag atattcggat
      421 ttctccctat cttcaagatt tgtttgatgc cgcaagaact gatggagttt acccgattgt
      481 cgcatccgga taccgaacaa cagaaaaaca gcaagaaatt atggatgaaa aaattgccga
      541 atataaggcg aaaggctaca cctctgcaca ggctaaagcg gaagcagaaa cttgggtggc
      601 cgtgccggga acgagcgagc atcagcttgg tcttgctgtg gatatcaatg cggacggaat
      661 tcattcaaca ggcaacgagg tttatagatg gctggatgaa aacagctatc gttttggttt
      721 tattcgtcgc tacccaccag acaagacaga gataaccggt gtgagcaacg agccgtggca
      781 ttaccgatat gttggcatcg aagctgccac agagatgtac aaccaagggg tttgccttga
      841 ggaatattta aaaccagaaa aatgagaaaa ggatgttatg ttatggacag aaaaagattg
      901 acacagcgtt tcccgttcct gcttccaatg agacgggcgc agagaaaaat gtgcttttat
      961 gcgggaatga gatttgacgg ctgtcgctat gcgcagacga taggggaaaa atcgctttcc
     1021 catttgctct ttgaaacgga ttgcgcatta tacaaccaca ataccggatt tgacatgata
     1081 taccaggaaa acaaggtgtt caacttaaag ctggcagcaa agaccttaaa cggcttattg
     1141 ataagaccgg gggaaccctt ttctttctgg tggctggtac gccatgcgga caaagatacc
     1201 ccctataaag acggccttac ggtgaccaat ggtaaactca ccaccatgtc gggcggcggt
     1261 atgtgccaga tgagcaattt actattttgg atgttcctgc atacgccatt gacaattatc
     1321 cagcgcagag gtcacgaagt aaaggagttt ccagagccaa acagtgacga gattaaaggg
     1381 gtggatgcaa ccatttcaga gggctggatt gatttaaaag tgcgaaacga taccgactgc
     1441 acctaccaaa tatgggtgac cttagatgat gagaaaatca tcggtcaggt gtccgccgac
     1501 aaagagcccc aagcattata caaaattaca aatggcagta tccagtatgt ccgtgaaagt
     1561 ggcgggattt atgaatatgc ccaggttaaa cggatgcaag ttgccttagg taccggggaa
     1621 ataatagatt gcaagctgct ttatacaaac aaatgcaaaa tctgttatcc cctcccggaa
     1681 agtgtggata ttcaggagga gaaccaatga gaaaaagtgt gggcattact gtttttggat
     1741 gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc ggattttcat attatcccta
     1801 cgctgattag cgatgcgata tcggcagaca acgcaaaatt ggccgctggc aatcaatgcg
     1861 ttagcgtagg ccataagtcc gaggtttccg aggcgacaat tcttgcgctg agaaaggtcg
     1921 gggtaaaata catttctacc cgcagcatcg gctgcgatca cattgatacg actgccgccg
     1981 agagaatggg aatctcggtt ggcacggttg cgtattcgcc ggacagcgtt gcggattatg
     2041 ctttgatgct gatgctgatg gccatacggg gtgcaaaacc caccatgcac gccgtggcgc
     2101 aacaagattt cagattggat cgtatccggg ggaaagaact gggggatatg actgtgggag
     2161 ttattggaac cggccatatc gggcaagcgg tcgtcaaaag gctgcgggga tttggatgcc
     2221 atgtgctggc ctatgataac agccgaaaaa tggatgcaga ttatgtccag cttgatgagc
     2281 ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct ttgtgcggat acccgccatc
     2341 tgatcggtca gaagcaaatt ggagagatga agcaaggcgc atttttaatc aacaccgggc
     2401 gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt ggaaagcgga aagctgggtg
     2461 gtgcggcact ggatgtgttg gagggcgagg atcagtttgt ttataccgat tgctcgcaga
     2521 aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat gccgaatgtg atcatcacac
     2581 cccatacggc gtactacact gagcgtgtgc tgcaggatac tacagaaaaa acaatcagga
     2641 attgtctcaa ttttgaaagg agtttacagc atgaatagaa taaaagtcgc aatcatcttc
     2701 ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg caatagaaat tgctgcgaac
     2761 attgatacgg aaaaattcga tccgcactac atcggaatta caaaaaacgg tgtatggaag
     2821 ctatgcaaga agccatgtac ggaatgggaa gccgacagtc tccccgccat actctccccg
     2881 gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg aatacgaaac acggcgtatt
     2941 gatgtggctt tcccggtttt gcatggcaaa tgcggggagg atggtgcgat acaggggctg
     3001 tttgtattgt ctggtatccc ctatgtgggc tgtgatattc aaagctccgc agcttgcatg
     3061 gacaaatcac tggcctacat tcttacaaaa aatgcgggca tcgccgttcc cgaatttcaa
     3121 atgattgata aaggtgacaa gccggaggcg ggtgcgctta cctaccctgt ctttgtgaag
     3181 ccggcacggt caggttcgtc ctttggcgta accaaagtaa acggtacgga agaacttaac
     3241 gctgcgatag aagcggcagg acaatatgat ggaaaaatct taattgagca agcgatttcg
     3301 ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg atttgattgt cggcgaagtg
     3361 gatcaaatcc ggctgagcca cggtaccttc cgcatccatc aggaaaacga gccggaaaaa
     3421 ggctcagaaa atgcgatgat tacagttccc gcagacattc cggtcgagga acgaaatcgg
     3481 gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat gcagagggct tgcccgtgtt
     3541 gatctttttt tgcaggagga tggcggcatc gttctaaatg aggtcaatac cctgcccggt
     3601 tttacatcgt acagccgcta cccacgtatg atggccgccg caggaatcac gcttcctgca
     3661 ctgattgaca gcctgattac attggcgtta aagaggtgat aagtatggaa aatggttttt
     3721 tgtttttaga tgaaatgttg catggcgtcc gttgggatgc caagtacgcc acatgggata
     3781 acttcacagg aaaaccggtg gatgggtatg aggtgaatcg catcatcggc acaaaggccg
     3841 tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc gcttggctat ggcttgcttt
     3901 tatgggatgg atatcggcca agaactgcgg tggactgctt cctgcgttgg gcagcgcaac
     3961 cggaggacaa tctcacaaaa gaaaaatttt accccaatat agagcgagcc gagttgatta
     4021 caaagggtta tgtggcctca caatccagcc atagccgtgg aagcgcaatt gatcttacgc
     4081 tctaccacct ggatacaggg gaacttgttt caatgggaag taacttcgat tttatggacg
     4141 aacggtcgca tcatacagca aaagggatag gggatgcaga ggcacaaaat cgaagatgct
     4201 tgcgtaaaat catggaaagc agcggatttc agtcttatcg ctttgaatgg tggcactata
     4261 agttgattga tgagccatac cccgatacct attttaattt tgccgtttca taatgaaagt
     4321 attggttttg tatcagattt tggatttgat aaaatctatg ctggtcatgg acaatctttt
     4381 actggaaagt ggttaaagca tcttttgtaa tacattgcaa attatagtat tcaatctgcc
     4441 cagcggcaga aaagaaaaaa accgctgttg ggcagacctt tcccacgccc attttgtatt
     4501 ttgctggcgg cggttttgag aaatcaaggc cgccgttctt ttctggcagc aaaaggtgtg
     4561 gcggcccata ggaggatgcc gctatgctgt tttgctattg ccatgctcta atccacttcc
     4621 accactcaaa aaaagccttg cccctc
//
LOCUS       DAYYKN010000174         4589 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000174 DAYYKN010000000
VERSION     DAYYKN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.176"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHBB7_002668"
     CDS             186..2855
                     /locus_tag="ACHBB7_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM9210555.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACHBB7_002669"
     CDS             complement(2946..3560)
                     /locus_tag="ACHBB7_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9210556.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3679..4218
                     /locus_tag="ACHBB7_002670"
     CDS             3679..4218
                     /locus_tag="ACHBB7_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210557.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4215..4553
                     /locus_tag="ACHBB7_002671"
     CDS             4215..4553
                     /locus_tag="ACHBB7_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM9210558.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1419 a          790 c          860 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa ataacggtgt
     3601 acatttttaa aaggagatgt ataatcgaga tttagaaagc gttttcctca aaatttaatg
     3661 aattaggaag tgattactat gaaaaaatta tttagcgcac tttttattct gttatttggc
     3721 tttattggta ttgggacttc tggaaatata gttaaagcta gcacgatggt tgaacaaaca
     3781 agtgaaatgg caaaaacttt tagtcaatta acaccagaag aacaaagtta cttcaaatcc
     3841 aaaggattta gtgataaaga tactttttac actcaaaaga tagagcctca agatccgaaa
     3901 acacgttttt ctgcaaatgt attgcggtta accgcatcta ctaaaaaaat tactaatacg
     3961 caggggcgga cagaatatat tataacaagt cccaattcgg cgatgtatcg tatttctaca
     4021 cgcttgagtt taggtagtgc aaaatcatat actcaaacag taaatctagc taattcttat
     4081 ggttatggtg gaggaatgta ttttacattt actgggaaaa gacaatatta cagtgtaaaa
     4141 ttagctatgc aagtttataa tacatttggt gtagggtcag tctcttgcac tgcgggagga
     4201 ttgacaatag gaaaatgata atttcttact atgtcttaaa tagaagtgat gatgaaggac
     4261 tcttcgtttt tcaatcatta catgaggata cgatgctaaa agagttttat aatcggatta
     4321 agaataaaca gatagaaaca aatttatatt ttttaagtga ctctatcttg ccttttatta
     4381 gcaaccctaa aattcttaac ttgttgcaga caaaagattt gaaaagttta ccgattattg
     4441 tcattgatga ggaaattgta aaatatggcg cttatctaag cgtagaagaa ttagaaaaat
     4501 taactaacat tggaatgagt attcaggagg ataatggggt acatggaaca tagaatgaat
     4561 atgcacagtt gagaacggaa tttgacatt
//
LOCUS       DAYYKN010000175         4455 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000175 DAYYKN010000000
VERSION     DAYYKN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.177"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..115)
                     /locus_tag="ACHBB7_002672"
     CDS             complement(<1..115)
                     /locus_tag="ACHBB7_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM9210559.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..698
                     /locus_tag="ACHBB7_002673"
     CDS             246..698
                     /locus_tag="ACHBB7_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210560.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            712..1401
                     /locus_tag="ACHBB7_002674"
     CDS             712..1401
                     /locus_tag="ACHBB7_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210561.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1526..3877
                     /locus_tag="ACHBB7_002675"
     CDS             1526..3877
                     /locus_tag="ACHBB7_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM9210562.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3957..4352
                     /locus_tag="ACHBB7_002676"
     CDS             3957..4352
                     /locus_tag="ACHBB7_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210563.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1565 a          573 c          885 g         1432 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      601 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      661 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      721 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      781 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      841 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      901 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      961 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
     1021 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
     1081 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
     1141 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1201 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1261 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1321 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1381 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1441 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1501 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1561 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1621 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1681 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1741 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1801 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1861 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1921 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1981 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     2041 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     2101 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     2161 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     2221 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     2281 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2341 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2401 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2461 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2521 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2581 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2641 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2701 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2761 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2821 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2881 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2941 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     3001 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     3061 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     3121 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     3181 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     3241 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3301 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3361 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3421 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3481 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3541 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3601 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3661 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3721 tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt
     3781 tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt ctgaaaaaat
     3841 tattcaattt caagaaaata gaagtaaact taattaagaa tcgtttacta tttaaacagt
     3901 gtccttttcc tttttaaata tatacgataa aataagtata tgaattgtaa aaggacatga
     3961 tgattatgga agaaatagaa tggaataatc tgactcaaaa taataaaaat aattctggat
     4021 gtggaattat ctttatgttt gttatagttt tgggaatagt agtactttta ataactgtca
     4081 agatagtaga ttactttgcg aatactcctg aagaacaaat acagagaaaa cttcctgagt
     4141 ttgaaagagt accaccttat gatccaagag ataattcatt tcaagtaaaa aaattaaaag
     4201 agtctccaaa tgaaatgtgg tttgaagtta cagttgaatc aagcgacttc aaagaaaaaa
     4261 agaaagaata caattatatt gtaaaggcaa aagtatcaaa aagatttatt tttaacccta
     4321 aattagaaat atatagtgtt gtaatagaat aatagtgtta aaaaaattgt aaaagttaaa
     4381 gaagtcaaat tctgatgtga aaaataaaat ataccatgga agacataaaa gaaaaactgg
     4441 ataactagtt tattt
//
LOCUS       DAYYKN010000176         4434 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000176 DAYYKN010000000
VERSION     DAYYKN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.178"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACHBB7_002677"
     CDS             complement(543..2048)
                     /locus_tag="ACHBB7_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9210564.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACHBB7_002678"
     CDS             complement(2045..2731)
                     /locus_tag="ACHBB7_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9210565.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHBB7_002679"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHBB7_002679"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM9210566.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAYYKN010000177         4426 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000177 DAYYKN010000000
VERSION     DAYYKN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.179"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..1830
                     /locus_tag="ACHBB7_002680"
     CDS             199..1830
                     /locus_tag="ACHBB7_002680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM9210567.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1833..2912
                     /gene="uxuA"
                     /locus_tag="ACHBB7_002681"
     CDS             1833..2912
                     /gene="uxuA"
                     /locus_tag="ACHBB7_002681"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9210568.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPACEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2986..4413
                     /gene="uxaC"
                     /locus_tag="ACHBB7_002682"
     CDS             2986..4413
                     /gene="uxaC"
                     /locus_tag="ACHBB7_002682"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM9210569.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
BASE COUNT         1536 a          611 c          919 g         1360 t
ORIGIN      
        1 atgaaaaaaa gaaaaaataa gtttcgataa aatcagtcca aaaagaagga gtattttatt
       61 tttctgaata aaaaatacaa tcattctgat atggtagaaa aaagcaagtt tattcccttt
      121 ttatattgac aacggttaca atgggtttta aaatataaaa tgtatcaaaa ctaacatatc
      181 agtttgagga ggttaattat ggtttcgatt aaaaattggc aaaattctat cgaagaattc
      241 aatgaaatgc agattctact gccgtgtttt gatgtcaaag aagttaagca agaaggccag
      301 caacatccag tatggcttca ttttggtgga ggaaatcttt atcgaggatt tcatgcagaa
      361 attgctcaat gcttagcaga tcaaaatgaa ttagaaacag gagtagttgt gtgtgaaaca
      421 tttgatgaag aagtagtgga aaaagcttat catgactata acaatgacat tttggaaatt
      481 gttatgagtg gagatggtga actcaaaaaa agacttttgg cagtaacggc tgatagttta
      541 tattgcaatc cgaagaatat agcggactat caaagagtgg ttaaatattt tgaagatcct
      601 aaattacagt ttacaacgtt tactattact gaaaaaggtt acagtttaaa agattctaaa
      661 gggcaattct tttcggtagt caaagctgat atcaaatctg gacctagcaa tcctcaacat
      721 acgatgagta ttgtaacatc cttgttatat agcagattta atgctggaga gttgccgatt
      781 gcgatggttt ctactgataa tttctcacag aatggtcaac gttttcaaga tagtattttt
      841 acgattgcag aagcttggca aaaagaagga tatgtttctg aaaaatttat tgcttattta
      901 aaggataaag aaaaagtgac ttttccttgg agtatgattg atcgaattac gccaaatcct
      961 tcagagaatg taaaggaaga tctattgatg atggggctcg aggatttaga tatcattcac
     1021 acaaaaaaac ataccaatat tgctgctttc actaatactg aggtagttca ttatttagtt
     1081 attgaagata gttttccaaa tggccggcct aaattggaaa aggctggtgt gattatgaca
     1141 gatagagaga cggttgataa agcagacatc atgaaggtta cgacaagctt gaatccactt
     1201 catactgctt tggcagtaac aggttgtctg ctaggatatc aatcaatttc agcagagatg
     1261 caggatgatg atttggttgg tttaatcaaa ggaataggtt atttagaagg tttaccagta
     1321 gttgaaaatc caggcattat tgatccaaaa caatttattg atgaagtaat taataaacgt
     1381 ttgcctaatc cctatattcc agatacacca caacgaattg caacagatac ttctcaaaaa
     1441 gtagcgattc gctatggtga aacaattaaa aagtatgtcg aaaaagacgg aaatgcagaa
     1501 aatctaacat ttattcctat cgctattgct gcttggttac gttatttact ggcagtcgat
     1561 gatcaagggg aaaattttgt gccaagtcct gatccgttat tggtagagtt gcaggataaa
     1621 ttatcagtat tgaaattagg tgaacaagat ccttcaaaga ttcattgtgc ggtacaggaa
     1681 attttagaaa ataaagaaat ctttggttta gatttatatc aagttggggt aggaaagaaa
     1741 actgaaaaaa tcttttgcga aatgctagta ggtcctcaag cagttcgtaa aacaatcaat
     1801 aaatatataa cagaagttgg aggaaagtaa ctatggaaat gacttttaga tggtatgggg
     1861 caaatgaaga aaaaatctca ctacaagaaa ttcgtcagat cccagcaatg aaaggaattg
     1921 ttggaacatt aatggatata ccagccggag aggtttggcc gagagaacga attaaatctt
     1981 taaaagatga agtcgaagcc aatggactta ccttgaaagt tattgagagt gttaatgttc
     2041 atgatgatat caaaattggt ttaccaacac gagatcaata cattgagaat tataaacaga
     2101 cgattcgcaa tttggcagaa tttgggattg aggttatctg ttataatttt atgccagtat
     2161 ttgattgggt aaaatcggat ttggattatc gtttagatga tgggtcttct acgttagcct
     2221 atatttcaaa aaatatccct actgatccgg aggaaattat tagagaagta gaaaattcta
     2281 ctaataattt tgaattgccg ggttgggaac ctcatcggtt atcacaaatt aagcaattat
     2341 tagaagccta taaagaagtt gatgaagaga aattacgaga gaactttgca tattttctca
     2401 aagcaattat cccggcatgt gaagaagttg ggattaaaat ggcagttcac ccagatgatc
     2461 caccatattc cattttcgga ttacctcgta ttgttaaaaa tcgtgacgat ttagattggt
     2521 tgtgtaatgt agtggattct ccttctaatg gtattacatt atgtactgga agtattgcag
     2581 aacgaccaga taatgatgtc tatgaaattc tatctgaatt ttgtcaacgg gatcggattc
     2641 cttttgcaca tgttcgtaat atcaaatatt taagtgacaa tggactcgat ttttacgaag
     2701 cgccgcataa atcagaatat ggttctctag atatgtataa gatattaaaa acaatgtacg
     2761 ataatggatt taatggctat attcgtccag atcatggtcg gatgatttgg ggagaaaccg
     2821 gtagacctgg ttatggatta tatgaccgag ctctaggtgc agtttattta aatggtattt
     2881 gggaagcgct tgaaaagggg gcgagtaaat aattatataa gggagtttat ttagaggaaa
     2941 aattttctct tctaaaaact aacggaagga aaggagtgtt taaatatgtt tttagataag
     3001 aactttttat tagagaatga aatagctaaa agactttttc ataaatttgc aaaacaccaa
     3061 ccaattatcg actatcattg tcatttagac ccagcatatt tgctagaaaa taagcaatac
     3121 aagaatttga ctcaggtatg gttaaatgat aaaggagctg gagatcatta taaatggcga
     3181 ttgatgcgag ctaatggtat agaagaagag ttgatcagtg gtccaggtga tgattatgaa
     3241 aaatttttag cttttgtaaa aacagtggag aaggcgccag gtaatcctat ttttgaatgg
     3301 tctcatttgg aattacaacg ttactttgat attgatttga caatttctga gaagaatgca
     3361 ccagaaattt ggcgacgtgc taacgaaaaa attcaaacgg attcctatcg cccgaaagaa
     3421 ttgttggaga tgatggatgt gaaagccatc tgtacaacgg atgatcctac agatgacttg
     3481 tggtatcata ctgagctttc taaacaggat aattctttca aggtattacc tactttccgt
     3541 ccagataagg tctggagtat caaatcatca ggcttttcga actatatttt aaaacttagt
     3601 caaacagcgg ctataccaat caaagacttt aatgggttga agcaagcatt gagccaacga
     3661 gtggatcatt ttcatcaaat tggctgtcga ttagcagatc acggtttgaa tacttatttt
     3721 tatgaaatag cagatgaaaa aatactcgac aatctgttaa taaaagcttt agcaggaaaa
     3781 acggatttcc atttatcaga aattgcacaa tttgttacag gtattcaatt acatttgatg
     3841 tctgagtata caagatatgg ttggacgatg caaatgcatt taaatgcttt aagaaataat
     3901 tcaagtatat taaaggaaca aattggagta gatgtcggtg gtgattcgat gggggatcaa
     3961 gctagtttga cacaaaatgt tgtagcattg ttcgataccg ctgaaaagag tgggtgctta
     4021 ccaaaaacaa ttttatattc attaaatcca actgattggc ttccactagc aacagcaatg
     4081 caaagcttcc aagggggtat gattcaaaaa atccaattgg gatgtgcttg gtggttcaat
     4141 gatacagctg aaggaatgcg tcaacagttg actacgatgg cacaacaaag tttgttgtcg
     4201 aattttacag gtatgttgac tgattctcga agttttctaa gctatcctcg ccatgagtat
     4261 tttcgtcgag tattatgtca attactaagc gagtgggtta aagcaggccg aattccagat
     4321 gatgaaatgt atcttagtca gatagtggag aatatttgtt atcgtaatgc gtataattat
     4381 tttaattttt ttgaaatcgg aagtagaaaa taatacttga aataaa
//
LOCUS       DAYYKN010000178         4416 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000178 DAYYKN010000000
VERSION     DAYYKN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.180"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..913
                     /locus_tag="ACHBB7_002683"
     CDS             59..913
                     /locus_tag="ACHBB7_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9210570.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ACHBB7_002684"
     CDS             1010..3259
                     /locus_tag="ACHBB7_002684"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9210571.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="ACHBB7_002685"
     CDS             3262..>4416
                     /locus_tag="ACHBB7_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9210572.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          625 c          806 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DAYYKN010000179         4399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000179 DAYYKN010000000
VERSION     DAYYKN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.182"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="ACHBB7_002686"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="ACHBB7_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM9210573.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="ACHBB7_002687"
     CDS             complement(2017..2919)
                     /locus_tag="ACHBB7_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9210574.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="ACHBB7_002688"
     CDS             complement(2916..3608)
                     /locus_tag="ACHBB7_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM9210575.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="ACHBB7_002689"
     CDS             complement(3651..4292)
                     /locus_tag="ACHBB7_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM9210576.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1348 a          945 c          761 g         1345 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttacca
//
LOCUS       DAYYKN010000180         4361 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000180 DAYYKN010000000
VERSION     DAYYKN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.183"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..386)
                     /locus_tag="ACHBB7_002690"
     CDS             complement(<1..386)
                     /locus_tag="ACHBB7_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210577.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDF"
     gene            complement(594..1520)
                     /gene="glsA"
                     /locus_tag="ACHBB7_002691"
     CDS             complement(594..1520)
                     /gene="glsA"
                     /locus_tag="ACHBB7_002691"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM9210578.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1543..2970)
                     /locus_tag="ACHBB7_002692"
     CDS             complement(1543..2970)
                     /locus_tag="ACHBB7_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9210579.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(3001..4215)
                     /locus_tag="ACHBB7_002693"
     CDS             complement(3001..4215)
                     /locus_tag="ACHBB7_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM9210580.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1370 a          926 c          774 g         1291 t
ORIGIN      
        1 aaaaaatcca atacgatttt tttgattgta tatactttgc tcaaagaaat aaattcaaga
       61 tcagccagtt ctgtactcgt gaatatacca ttaaaataat aacttaaaaa ctttaaaaaa
      121 taagattgac cgtacagaaa ctgtgtgagc tcagaaagag caataccatg tttgaatacg
      181 agtttatact tatgcttctc tcttacttgt tcttttatac aatattctag acactcggct
      241 agactttcta gatcgtgaag gatcgttggt gtagtcaccc ttagagaaat agctgtttgt
      301 tcgatgtcta tttcttggaa ttcaaataat tgttcacaca gatacacttt tctaaatata
      361 tttcgctcga tgtaattttc cagcattcct gatacacgcc tccagttttt cttatgattt
      421 tatttaatca tatctgtaaa tcgtattttt gtttcctttt tatttagaaa agccctttca
      481 aaaaccaata aaaaaagaaa ctgggacaaa agtcaaacaa gctcacaatt tcagcctcat
      541 tgtaggtagc tttgagccgt ttggactttt accccagcct ctttcaagaa tggttattca
      601 aagatgtcag cgtctagttc tttgactaca tctttcaaca attggatacc agctgcgctg
      661 ttaccaaatg gatctagtgc cgggctgaag acacctatgc cgtaacgatt tggcacagct
      721 gccatcaacc cgccgcctac accgcttttt gcaggtacac caacatggac ggaaaactct
      781 cctgattcat catacaaacc agcagttgtc ataattgatt tgacgattgt agcactttct
      841 tcagtaatga tttgtttgcc attccaagga gcaattcctt tatttgccag cacagcagct
      901 aattttgcta tgcctgtagc ggtgacattg agggagcatt gtctgaaata agcatcgagt
      961 atctccggta cgtctccttc gatcatttga ttacctttca tatagtaagc aagagagcga
     1021 ttgatatcac ctgttcttga ttcagaatga tagatttctt cattcaatgt gatattagga
     1081 tcatcacaga tctctttcat aaatgacaag attcgctcaa atttttcatc tgcatcttta
     1141 ccggcaatca acgaggttgt tgcaatcgct cctgcattaa caaaaggatt cattggatgt
     1201 ttgcgatgat tgatttccat attcatgata gaattgaagg caaaaccggt tggttccgtg
     1261 ttgattgttt gaaagacttt ttctattccg ttgtctttga ttgctaataa caatacggga
     1321 acttttgaca tgctttcgat ggcaaagcgc acatcggcgt ctcctccctc aatcagttgt
     1381 tttgtatcca aatcataaat cgccatacct aattgtttgg gatccacttc agctaatgct
     1441 ggaatgtaat ccgccacttg tcctgtttga taatagtgga aattttttcc aatcaattgt
     1501 tctaattctt ttacttccat ttgaataacc agctttcatt ttttattttt ctgcatgctg
     1561 gaatttatga cggcatgcgt agatcgtaaa tggaataact aagacgataa gataagaaac
     1621 aatcagaatc gtttcataaa tcccatgctg tccagctgcg atactagcag gtggaacaaa
     1681 tgagatacaa aaagcaaaaa gtgaagtcaa aagacctaaa caagcaacga ttacttttac
     1741 aacttttccg cctggaacct gataggctct ttcaagactg tcatctttaa aaaccagctt
     1801 caaatagccc gcgaaaaaca taaagtacaa catcaggtag attacaacag tcaatgacat
     1861 cgctgcaaga aatgaaagat tattcccccc gccgcttaat gtaaagacgg ctgcccagat
     1921 tgttgcaacg atcccatttg cgatcatcaa atgagcagga acaccgtgtg tgttttcttt
     1981 tgcaaaatat tcaggaagta atccttcttc tgctgctacg tgaagtgctt ttgaaggaga
     2041 ggttacccaa gaagcaattt ctccgatgac acctaaacaa acaagaatcg caagaatttt
     2101 cgcaatccat gcaagatttg gatcaatgtg tcttaatagg aattctaacg attgaatcac
     2161 ccctgtatta agagacaaac catgttgagg tactgtagta gcgacggtca atccgccaaa
     2221 agtatctaaa atgattgcca gaataaccaa tacaaacatt gctaatggat aatccttttt
     2281 agggttttcc atttcatttg catgactggc agatgtttct acgcccatat atgctaaaat
     2341 aaaggaaaca aaaacaacca gcgtaggcaa tttcgaaaaa tcaggaatca atgttttcat
     2401 agacagtatc gtttgtagtg gagcgcctga agcgacataa tggatcccta agccaaacaa
     2461 gactaatgct ggcaacaaga taccagctac aaaaccgatc cgagcaatca atgaagtgta
     2521 tttggttcct tttaactgtg agagtaaaat agcccagaaa ataatcaata ggattgccaa
     2581 tttcagatat ttattgtttt gtatagcacc aatacccgtt gcataggata atgcaccagt
     2641 taagaaatac agcatggtca agtagccaac tgttatctca aaccattgga aaaagattgc
     2701 ggcaaaaccg aatcgttttc ccaatgtgcg actgacccaa gtatagacac cgcctttttc
     2761 ccatcctttc accgttgcca tctctgctgc gcacaatgcc acagggataa accatagcaa
     2821 accgcctaat aacaagaaaa atactaagct gaagcctgag gtcgcgaatg tgggatattc
     2881 atataatgaa acgaccattg agatggtcat agccaaaaag ccaaagaacg ttaatttttt
     2941 tgcaggaatt ttcgttttgc tttgttgcat gaatcttcct cttttctatt tgaatttgtt
     3001 tcaattgagt ttgttgccaa agaagaaaaa gtccaatcct tcttcttgtt cttcttttgt
     3061 agtagtgatc gttgtaaagt gcatcgccga ttttaaaatc actgttgtca gcacataggc
     3121 atacacggca gtaatgatga caccaatcac ttgaatccct aagaagtacc agctgcttgg
     3181 attcgttagt aaatggctgt tagatgcaaa gatccctatc agaatcgtac ctgtgaatcc
     3241 gcccatgcca tgaacacccc acacatcaag tgcatcatcc cattgtttct ttttacgaaa
     3301 atcaacagca gcatgacaga cgatacccgc aatgatccca atcagcatgg cactttttgc
     3361 ttctacataa ccggcacaag gcgtaatggc tgccaatcca gcaacggatc ctgttagtac
     3421 gtcgacaaaa tcaaagccat ttccttttac ttttgcaatg atcagccaag taatcatcgc
     3481 aaatgcaaga gcgatgaatg ttgaaccaaa tgcattgact gcttgttcat ctgctcgaag
     3541 tgcaccaccg gaattaaaac cgaaccagcc aaaccaaagc agtcctgtac cgatagcagc
     3601 tgccataaga ctgctatgct gacttctttc tcctaaagga atctttcttt gaccaagtgt
     3661 gaggatacat gctaaagagc cgaatccagc tgtcgtgtgg atgactgctc caccagcgaa
     3721 atctctaaat cccatctgat ccaaaaatcc gccaccccat atccagtggc aaacagggaa
     3781 ataaatcagc agattccaaa aaataacaag tataatatag ccttttaaat tcaatctccc
     3841 tgcaaaagct cctgtcatca aaggaacggt aatcacgata aacatcagtt ggtaaagaaa
     3901 gaacattaaa aatggaatcg ttgcaccatg aaacatgttg gggaagcttg taacatgtct
     3961 catcaaaaag aaatctgccg ggtttccaat aacaccgccg atatcccgtc caaaggccaa
     4021 tccgaatcca ccaaataccc acataatcgt tgttatccca acggagagaa aagattgaga
     4081 catgatcgtt agtatattat tcttccgtgc taatccgccg taaaaaaaag ctagaccagg
     4141 agtcatcagg caaaccattg ctgtacataa aatcatgaat gctgtatctc ctgtattgat
     4201 aggtaatgta ttcatttttt tcgcctcctt tctacgatta cgagtataaa agaaggtgta
     4261 gttgtttttt tcttctgttt ttttgaatta atacttgcat ttataaaaga cagacaatta
     4321 ataaaagctt gatgtgttgt gctctttttc gtttttcaca c
//
LOCUS       DAYYKN010000181         4200 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000181 DAYYKN010000000
VERSION     DAYYKN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.184"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..365
                     /locus_tag="ACHBB7_002694"
     CDS             24..365
                     /locus_tag="ACHBB7_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM9210581.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            674..1717
                     /gene="pheS"
                     /locus_tag="ACHBB7_002695"
     CDS             674..1717
                     /gene="pheS"
                     /locus_tag="ACHBB7_002695"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM9210582.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            1722..4142
                     /gene="pheT"
                     /locus_tag="ACHBB7_002696"
     CDS             1722..4142
                     /gene="pheT"
                     /locus_tag="ACHBB7_002696"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM9210583.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
BASE COUNT         1451 a          709 c          948 g         1092 t
ORIGIN      
        1 tactctggga agaggtgagt atgatggagc taacacagac agaattcgtt ttatgcccta
       61 aatttgaaaa aacattctct attttaggaa aaaaatggaa cggattgatt atcgatgtac
      121 tactcgaaaa cggtccgcaa cgctttgttg agcttgcggc aaaaatcccg atggtcagtg
      181 atcgagtatt agtcgaacgc ttgaaggaat tagaaaaaga aggaatagtc actcgaaact
      241 ttgcctgtag cgaaaagagc cgttcagaat atctcttgac tgaaaaagga gcagacttgc
      301 aaaaagcgat gcgtcaaata caagtttgga gcgaaaaatg gatcacagag gaagaatgcg
      361 gttgattttg taaaaaaaat cttgtaaact atagtccata cgaataagta aagaacgatg
      421 acgaaaaaga gtagtttggc taaaccagtt cagggagatc tgtcgcagac tgaaagcaga
      481 tcctgtgttt gaaaaatgaa gttcactttc cgagttcgtc tgataaaatc agaccggcaa
      541 ttgtcgttaa cgaagtaaga gtgcggtgta agtcatttat ccgcaaatta ggatggtaac
      601 gcgaagacct cgttccttaa caggaatgag gtctttttgt tttgtatttg gaatttaaag
      661 gaggaacaag cacatgtctc tacaagcaca attagagagc ttgaaagaag caacgctcgt
      721 caaaatcaca gcggcacaag atttaaatga attaaatcaa atacgtgttg aaacgttagg
      781 aaaaaaagga ccgatcacag aagtattacg tgggatgaaa gatctaagtc cagaagagcg
      841 accaaaagtc ggaagtttcg caaatgagat ccgagatctt ttgacagaag agatcgagcg
      901 aaaaaaacaa gtattagaag aagaagcgtt aaatgcagct ttggcaaaag aaacaataga
      961 cgtgacacta cctggtagaa aagtaaaaca tggtacacgt catatcgtga cacaaatcat
     1021 ggaagaaatt gaagatattt ttgtgggtat gggctatcag atcgtagaag gatatgaagt
     1081 agaatctgat cattacaatt tcgaacggat gaatctgcca aaagatcatc cagcaaggga
     1141 catgcaagat actttctata tttcagacga gatattgatt cggacacata cttcaccagt
     1201 ccaagcacgg acaatggaaa aacatgattt ctctaagggt gctctacgaa tgatctcgcc
     1261 agggaaagtc ttccgcagag atacagatga tgcgacccac agtcatcagt tccatcaaat
     1321 tgaagggcta gttgttgata aaaacatcac aatgggcgac cttaaaggaa cattagaagt
     1381 cgtaatgaaa aaaatgtttg gggaagatcg tgaaattcgt ttgcgtccaa gttatttccc
     1441 atttacggaa ccatcagtag aagtagatgt cagctgtttc aaatgtggtg gtgccggttg
     1501 taacgtatgt aaatacactg gatggatcga aattttagga gctggcatgg tacacccgaa
     1561 tgtgttgaag atgtcaggaa tcgatccaga agaatattca ggctttgctt tcggccttgg
     1621 accagaccgc gtagctatgt tgcggtatgg agtgaatgat atccgtaatt tctatcaaaa
     1681 tgacttacgt ttcttaagtc agttcaaggg ggagtaagaa aatgttagtt tcatataaat
     1741 ggctcaatcg ttatgtagat ttatcgaatg taacaccaaa agaattagcg gataagatgt
     1801 ctgtgacagg aatcgaagta gagggagtaa cggttccaga agaaggcttg aaaaaaatcg
     1861 ttgtcggtga agtcaaggaa tgcgtaccac atccagattc tgatcacctt tctatttgtc
     1921 aagtcgatat tggcgaagac gaactgtctc aaatcgtttg tggagcgcct aatgtgaagg
     1981 ttggaatcaa agtgattgtt gcacttccta actcacgtat cgctggtaat gtaaaaatca
     2041 aaaaaggaaa aatgcgtggc caagtatcaa atggcatgat ttgctcatta caagaaattg
     2101 gctattcaga tagtgttata ccgaaagaat acgctgaagg tatctattat atgccacaag
     2161 aagctgtaaa tggcgatcca gtttttcctt atttggatat ggatgattcg attattgaat
     2221 tatctatcac accgaatcgt gcggatgcat tgtcaatgcg tggcgtagct tatgaagttg
     2281 gggcaattta tcgtcagact cctacattcg atgacgaaaa gctggtagaa tcctcacgtc
     2341 ctgctagcga taaaattcaa gtggaagttg aagataaaga agcagttcct gcttatcaaa
     2401 tcagaatcat tgaaggagta agaatccagc caagtcctca atggctgcaa aatcttttga
     2461 tgaacgaagg cattcgtcca atcaacaatg tagtagatgt gacgaactat attttgcttt
     2521 tattcggaca accactacat gcatttgact acgacaaatt gaaatcaaaa gagatctttg
     2581 tccgccgtgc aaaagaaaat gaaacattag taacactaga tggagaatct cgggaattga
     2641 caactgacaa tctcgtcatt actaacggca aagaaccagt tgctttagct ggtgtcatgg
     2701 gagggttaga ctcagagatc acgaatgaaa caacaacagt tgcactagaa tctgctttat
     2761 ttgatccaat ttcgattcgt cgtacagcaa aacaatttaa tttgcgtagt gaatcctctg
     2821 cacgttttga aaaaggaatc aaccatgcga ctgttggaga agccagtgag gttgctgcag
     2881 caatgatcgt ttctcttgcg ggtggggaag ttctacaagg cgcggtcaaa ggctcagaga
     2941 tcaaagcaga agacgtatca gtgaagattt cacttgaacg catcaatcaa tatttaggaa
     3001 catctcttgt cgttaaagaa gtaaatgaga tttttgaagc acttggattt agctatgaag
     3061 aaaaggagta tgtttattcc gtagtagtac caccaagacg ctgggatatc caaatcgaag
     3121 cagatattat cgaagaagtt gctcgtattt atggatacga ccagttgcca tctacacttc
     3181 caagtggaga aacggttgct ggaagcttgt caaaagaaca agaaactact cgtaaaattc
     3241 gtacaatctt tgaaggaagc ggattgagcg aagcaatcag ttatgcattg acaacagaag
     3301 aaaaatcaag acagtttgtt ataaatgaaa gtaatattac acgtttggac tggccaatga
     3361 gcgaagaacg ttctgtatta cgaatgaatt tgatcagcgg actattagat gatatcagtt
     3421 ataacactgc ccgaaaaaat acagagattg ctttatatga aattggtcgg gtgttcttcc
     3481 aagaaaatga tccacgtaca catctgccgc aagaagtaaa acatgcagca atcgctattt
     3541 ctggtatttg ggaagaaaaa gattggcaga cgaaagctca aaatgtagac tttttcacca
     3601 tcaagggtct tttagaaaac ttatttgaac aactaggcat cactgaagat gtccattatc
     3661 aaacagtgac agaaatgaag gaaatgcatc ctggaagaac tgctgcgatt taccttggcg
     3721 aaaaatttat tggttttgtc ggtcaagtgc atccaacgat tacaaaaagt tatgatattc
     3781 cagaaacata tgttgctgag tttgatttat ctgctatagt ggaagctgct caaaaaggga
     3841 tcgttttcga agcagtgaca aaattcccag cagtcagccg ggacatcgct ttgcttgtaa
     3901 aagaaactgt gacaaatcag gaattaacag aagttatccg ttctgctgcc ggaagatttt
     3961 taacagatat ccaattattt gatgtctatc aaggagaaaa cattgaaaaa ggacataaat
     4021 ccatggcgta tagcctgaca ttcgtcaacc cagaagcaac attgacagat gaagaaatca
     4081 ataaagcaat ggaaaaagtc gaaaaagcgt tggtagaaaa cctagaggca agtattcgct
     4141 aagaaggaaa atatagtaag taacgatcaa gtagcttttg aagaccatac atttgaaaaa
//
LOCUS       DAYYKN010000182         4190 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000182 DAYYKN010000000
VERSION     DAYYKN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.185"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHBB7_002697"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHBB7_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFM9210584.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACHBB7_002698"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACHBB7_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM9210585.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            2068..2322
                     /locus_tag="ACHBB7_002699"
     CDS             2068..2322
                     /locus_tag="ACHBB7_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9210586.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            2323..2640
                     /locus_tag="ACHBB7_002700"
     CDS             2323..2640
                     /locus_tag="ACHBB7_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9210587.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            3199..4134
                     /locus_tag="ACHBB7_002701"
     CDS             3199..4134
                     /locus_tag="ACHBB7_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210588.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT         1402 a          647 c          736 g         1405 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     1801 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcgacttctg
     1861 cctaattctg ttaaatggat gttaacggaa gtgtatggtg agtagttcac tcgtattttg
     1921 atatcgtttg tatatgtttt cagcgttcta agtggcttat tttgtccaat aataatatgg
     1981 agtggaatcc cttaagaaac accgttggca attgttgtta taacatgtta taatttattc
     2041 atcaaaaact aaaaggagtg tgataacatg ggaaaagaac taaaggttag aaagatcggc
     2101 aatagcgtgg gagtgatcct tccaagctct cttggattaa aaagcggaga cattatccaa
     2161 gccaaacaag aaggcaactt attcatatta gatactactc aaatagcaaa agaacacgat
     2221 cgcaaattga ttgaagaaag tttccaagac tttgagaaag gtttaacagt ttcagaaatt
     2281 gaaatggtaa aagctttcgg aaaatacggc tggagtgaat agatgaaaga gtacacgcta
     2341 gtttattcta aaagttttca gaccagcctt gaaacgataa tagaagaatg ggaaaatgag
     2401 cttgctcttt ctccagccag cataaaaaga tttgtaggtg cgatccagaa ggctttagaa
     2461 ctaaccaaaa attttccaac tatgcatgag gaagtttccg aaatctatgg aatggatatc
     2521 ccaacatatc ggatcgtgat cggcaaaagc tatgccttat tttatcggat cgaccatgaa
     2581 aaaaaagaga tacttgtagg acgtattttc caatctaaac aaatgaaact aggcttttaa
     2641 caataattag gaaaaagtcg ctcatcctct acaggtgggt gattaatgcc gctcgatatg
     2701 gttgttaacc gtcggttact cgaagattga tatataagac ttagtacctt tttattttgc
     2761 tccatgttat ggccgaaggt ctaaagatac aatcattagt ctagagaaaa attaagaacc
     2821 gtaggaacta cggggatcgc ttggtgaata agagacactg ctattagtga aaaaataaga
     2881 tatcaagtac gctctattcc caaaaatttc gtgactttac tcatgagagg ttcaagtagt
     2941 atatttttaa tcaaaattga cgtaactttt aataaatact acctaaataa ggtagtattt
     3001 atttttgtgt tttaaaagag gaagtccaat atatctatgg gaaacttctt aaatattatg
     3061 ttggtacatg aaaatataca tccctgctag actatttaca acattttact ttgtacgtat
     3121 tcttgtttta aaaggaatgg gataaaatat aggtacaata actatttgat gctaaatata
     3181 attaatggag gagagatagt gaacatactt aatgaaaaaa gatatgtatg gtttgctggt
     3241 aaaaaatata ttactacttt caaaagatta tccgagttaa tgcagcagac tgttaaaagt
     3301 gctatgcctg aaggatacaa gtttaatttt gttcaatctt cagtatctac tgaaagtatc
     3361 tatttttcaa ttctttctga gagtaggctt tttcatttta gaataagttc tcatgtaaag
     3421 aaaactgatg ttgatatata tacgttccgt acaaataatt ataaaaattt aaatgatttg
     3481 agtagaagta tcagtgaata tctcaagggg aatagctatc tcaagattcg attttcacat
     3541 atattaattt tactaccata tttgaatgag aatttcaccc taattcaagt agataagtct
     3601 ttcattgcct caggtaatca aggaaatatg aatattacaa gtaacaaaac gtcacgtgtg
     3661 ctattggcgt tgatgggtta cggaattctt tatagagatg accaaaatgg ccagattcta
     3721 ataaatcggg ctgggcgttc attattaatg aaatatagaa aatttatacc ggaagatttt
     3781 caaaacttac agttagaaaa catgcgttta gaagatttat ttagattagc agaagatgat
     3841 aagtatagat ttgaagtttt gaaaattcaa aagaatgaac tattgaataa gttatatcct
     3901 gagaatgcga taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct
     3961 aaaagattta gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt
     4021 acagatgtcg taatgataac agatatctat gtagaagatg aagatataat caaatatatg
     4081 aagcctgtga taaaacttgt taataaaaat aaaggaaccc gtggtgctag ttaatttttt
     4141 cctagagaaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
//
LOCUS       DAYYKN010000183         4163 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000183 DAYYKN010000000
VERSION     DAYYKN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.186"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACHBB7_002702"
     CDS             352..1041
                     /locus_tag="ACHBB7_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9210589.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACHBB7_002703"
     CDS             1028..2236
                     /locus_tag="ACHBB7_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9210590.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACHBB7_002704"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACHBB7_002704"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM9210591.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1368 a          718 c          842 g         1235 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt att
//
LOCUS       DAYYKN010000184         4150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000184 DAYYKN010000000
VERSION     DAYYKN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.187"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..636
                     /locus_tag="ACHBB7_002705"
     CDS             133..636
                     /locus_tag="ACHBB7_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9210592.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="ACHBB7_002706"
     CDS             763..1800
                     /locus_tag="ACHBB7_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic GMP-AMP synthase DncV-like
                     nucleotidyltransferase"
                     /protein_id="HFM9210593.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="ACHBB7_002707"
     CDS             1815..3512
                     /locus_tag="ACHBB7_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HFM9210594.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="ACHBB7_002708"
     CDS             3505..3966
                     /locus_tag="ACHBB7_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFM9210595.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
BASE COUNT         1451 a          523 c          858 g         1318 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa
//
LOCUS       DAYYKN010000185         4082 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000185 DAYYKN010000000
VERSION     DAYYKN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.188"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            558..3197
                     /locus_tag="ACHBB7_002709"
     CDS             558..3197
                     /locus_tag="ACHBB7_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9210596.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3365..4063)
                     /locus_tag="ACHBB7_002710"
     CDS             complement(3365..4063)
                     /locus_tag="ACHBB7_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM9210597.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1231 a          784 c          904 g         1163 t
ORIGIN      
        1 aaaaagccga gctctcgata agctcggctt tcttgtacct cggaaggatt caaataaaca
       61 gaaatgattg taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa
      121 acaaaaattt tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat
      181 tttcagacgt ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt
      241 cgtgatccaa acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct
      301 gggtgaaacc tgtacttcta acaactttgt ttgcttacac tgttctatcg agaaattctt
      361 ctgtctattt gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt
      421 ttgcaaatat aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta
      481 tttgaagtaa ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat
      541 tttaaaggag gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa
      601 cagaagcaga tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc
      661 agggattgaa taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa
      721 gcttgtccga ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg
      781 catttgctac agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga
      841 tcaactcggt attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc
      901 aagatatgaa taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt
      961 cagagcaact ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg
     1021 atgcccgttt gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa
     1081 gtgaagcagt tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc
     1141 aacttaatat ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgatcgtca
     1201 ctgccattgg catggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta
     1261 tgcaaaaaac acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg
     1321 ctttagcggc tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg
     1381 agatgttcat gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta
     1441 ttgttacgtt gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc
     1501 gacagttgcc agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag
     1561 gaacgctgac acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa
     1621 cgaatatcga agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat
     1681 gtacagatgt gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag
     1741 aagtagccat cgtaagggct gttgaagaga attatcatac aaaggcagaa ttagaagaaa
     1801 aatatccacg agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc
     1861 accaaatggg cgaaaaatac atctcaatca ccaaaggagc attcgatgtt cttgctccgc
     1921 gctttagttc aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg
     1981 cactgagagt gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt
     2041 ctgaagcact ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac
     2101 gtccagaaag taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga
     2161 tcactggaga tcatgtggtg actgccagtg ccatagcgaa agaattaggt atcttgaatg
     2221 atccgtcaga agcactttca ggatcagaac tgcatcaatt atcagacgaa gaattagatg
     2281 cgcgtgtcaa agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg
     2341 tcaaatcttg gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg
     2401 caccagcgtt gaaagcaagt aacgttgggt gtgccatggg gatcacagga acagatgtgg
     2461 cgcaaagtgc cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagacgctg
     2521 tcagtcaagg acgttcggtt tatcagaata ttcgtaaagc tatcaacttt ttgctaagct
     2581 gtaatatttc ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat
     2641 ttacagctgt gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt
     2701 taggaagaga gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag
     2761 ggatctttgc tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg
     2821 taaccttgtt tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg
     2881 caagtcagca tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc
     2941 atgtgttcaa tgtacgcagc agccaatcga tcttcaaagt caatttagcg accaataaag
     3001 ctttggtaga aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat
     3061 ttacgcaaga actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga
     3121 ttttatctat cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag
     3181 cagaagaaga ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca
     3241 aaaaggaggt gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc
     3301 ttttactgcc tctcgataaa cggtgttctc ctgccacccc ctcggtcaca agtcacactc
     3361 tcttttaaat atcttttggc aattgttcat aaaatgctgc ttttgttgca gaaatgtatg
     3421 ggctgagcca tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa
     3481 atgaaagatc taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag
     3541 tgatcgtatc cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtaaataa
     3601 aatagctttg agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc
     3661 ataatgttgt aaagatcgag atcagcacag ctaagctgat cactccgccg ataaacaaac
     3721 ctttgaatcc acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa
     3781 tatcaagata agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa
     3841 ttccgccacc gcctcctgaa ctaccgcctc ctgaagttgt cgcttctgag acgctcgctg
     3901 atccatcata tatggtggtg gcaaacaaga taataggaat cataataaga agaatagtag
     3961 caataccaat caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta
     4021 gactctcttt tgcttggcgt tttaattctg cagaagattg catgattttt ccctcctttt
     4081 ct
//
LOCUS       DAYYKN010000186         4066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000186 DAYYKN010000000
VERSION     DAYYKN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.189"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACHBB7_002711"
     CDS             85..1434
                     /locus_tag="ACHBB7_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9210598.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACHBB7_002712"
     CDS             complement(1562..2152)
                     /locus_tag="ACHBB7_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM9210599.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHBB7_002713"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHBB7_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM9210600.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAYYKN010000187         4044 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000187 DAYYKN010000000
VERSION     DAYYKN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.190"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            310..618
                     /locus_tag="ACHBB7_002714"
     CDS             310..618
                     /locus_tag="ACHBB7_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM9210601.1"
                     /translation="MFQNKGQRYVTKGVMDSLLVELQTLCWNLIDQNVQKKLPLDYLQ
                     IFEFSIEKGNQKLVHRQEEPEARKEYLISPKLRLESVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            645..944
                     /locus_tag="ACHBB7_002715"
     CDS             645..944
                     /locus_tag="ACHBB7_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM9210602.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGTLPKNVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVCGLLVEGRLVWSQIGGTNE"
     gene            1118..1663
                     /locus_tag="ACHBB7_002716"
     CDS             1118..1663
                     /locus_tag="ACHBB7_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM9210603.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEEKEKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFSRGNDEQSLE
                     ILTTIFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLSDHSLLFQ"
     gene            1819..2115
                     /locus_tag="ACHBB7_002717"
     CDS             1819..2115
                     /locus_tag="ACHBB7_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210604.1"
                     /translation="MKVDGECLTSSYKSIYEFFEEKGLDTDLLYQFYIAFRGQQLSFP
                     MKMYDRELVAQKIETMIQENHSIDSRKLTDRYGFSTRWIQSIIRQQQKEQHVHG"
     gene            2108..2998
                     /locus_tag="ACHBB7_002718"
     CDS             2108..2998
                     /locus_tag="ACHBB7_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HFM9210605.1"
                     /translation="MANEETVGLRIIEEAFKLPMVKVDRRAFLIQTFQGNVSDIEGLI
                     EKGPQAFLSKEMLDREAQKCIQSITLKSSAVSLATGLPGGIAMAATIPADLLQFFGYS
                     LRLAQEISYIYGFKDLMGETGEFSEEAKNTLILYLGIMLGVTSAGSAVRAMSGKLSTQ
                     ALKKIPQKALTQTLYYPVIKRVLGIFGTKLTKNTFAKGISKAVPVVGGVVSGGINYLS
                     MKPMATKLQTELGKNVAYTEKNLQQDLDILEGEFEEVSSDIVSSDTVLSQLERLSHLL
                     ETNMITEEEFQQLKQELLKK"
     gene            3080..>4044
                     /locus_tag="ACHBB7_002719"
     CDS             3080..>4044
                     /locus_tag="ACHBB7_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM9210606.1"
                     /translation="MKAKRLFLLGTILLLGGCSTVSQNEQKKQVDSTTYETETTRKES
                     STKEIETTTQETKTTESSSKATSSDTSDFQIKQELWDTNKASQLATFVPQWGKTLGQE
                     YKSYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGNAGFNLVAVYSDV
                     ETGEYLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFTDIINGQT
                     PQTPVVEESTQQATTTTTQSTSSSGISNWEAAKETVLNNKALWIYESPDKNEANTNII
                     ESTQYRKDLMQDENGEFYPVDISTDDIGNTVMNGGKQFRVYTDGR"
BASE COUNT         1507 a          580 c          784 g         1173 t
ORIGIN      
        1 ttatttataa taacaatagt agtataacat taataggata gttcattaag caaaattaag
       61 tatcttagtt actaataact atataaattt taaacttgta gtcgcaattt agtatagagg
      121 tactaaatat gaaaactcca tacttttaga aaagcatttc ttatatttag ttgtgatgtc
      181 tattcagtta attgaaataa ataatatagc aatgaaatag agataaattc aaatatatat
      241 tatatccatg aagtccaatc ttctttatag aagattgggc tattttttac gacaaaggag
      301 ggaaaagaaa tgtttcaaaa taaaggacaa cgttatgtca caaagggagt aatggacagt
      361 ttactggtgg aattacaaac tttatgttgg aatttaatcg atcaaaacgt tcaaaagaag
      421 cttcctttag actatcttca aatctttgaa ttttcgatag aaaaaggaaa tcaaaagttg
      481 gttcatcgtc aagaagaacc ggaagcaaga aaagagtatc tgatttcacc taagcttcgt
      541 ttggaatcag tgtctcaaaa aatatgggtc attgattcgg gtgagtatca aacgatgtta
      601 ttaccagaag aatactgata tctaaaagaa aggagcgaaa tgacttgtta gaaacggcta
      661 tcgaagtact ggaaaagtgt gcacagctag tcactactcc tgaagagtgg ggctatgaat
      721 ccgttacgat ggaaaaagag gaaattgaaa tgggtacgct tcctaaaaat gttcatctac
      781 cacgtttagt gatgacacat ctttatattt attgcgcacc agaagatgga aaagactatg
      841 tagtttattt cattacagat attaccagcc agcgagaatt tgtttgtggg ttactggtag
      901 aaggacgatt ggtttggagt cagattggag gaacgaatga ataagatcga ttatttgaga
      961 atgattgcga aatgtttaga agatatcctt accgcgattt atcgaattct gaagaaaaaa
     1021 ggagaaagaa tatgaaatat tttcaactag tattgacact tataacggca atggtaattg
     1081 aattgcttga acaaaaatta gcataggaga aaaagagatg attgaaaaag aattagtgac
     1141 cacacaaaca gaagtttggc gagatcctca tcatacccat cgctatgttt tcaaacgaca
     1201 atgggcaatc aaaggaaaag aagagaaaga aaaattagca gtagtcatta ccattcgtcc
     1261 aacagataca gaaccattta cgaatgattt gagtatgtta ttaatcgaaa agaatatccg
     1321 acaattggga tttactggtt ttattgccgt gaatcttttt tcgtatacaa aagcgaaaag
     1381 tcctcgggta ttttctcgtg gcaatgatga acaatctttg gaaatattaa cgaccatttt
     1441 tacagaaaag aaaatcaaca ctattatttt tgcttgtggt tcgattacgg caacgagtca
     1501 agtggcttat gaacaagcaa agaccattta cgatcaattg agtgcgaaac aaaagaagat
     1561 gaccaaagta ttgataaatg cagaaggcaa gttatctcac ccattaagta ttcatgttcg
     1621 taatgaatgg caattgtcgg atcattcttt gttatttcaa taaacgaaaa taagtattca
     1681 atgtatatgt ttctttttct gttattttac ttgttctttt gcagactaaa caacttgtaa
     1741 aacctcttgt tatatgcatt atcaatgaag gaatcaatat gatattttta aaatgtcagg
     1801 ggaaatggag gtgagcaagt gaaagtagat ggggaatgtt tgacgagttc gtataaaagt
     1861 atctatgaat tttttgagga aaaaggacta gacacggatt tactttatca gttttatatc
     1921 gcttttcgtg ggcaacagtt gtcgtttcca atgaaaatgt atgaccgaga attagtcgct
     1981 caaaaaattg aaacaatgat acaagaaaac cattccattg attctaggaa attaacggat
     2041 cggtatggtt tttcaacgag atggattcaa agtattatca gacaacaaca aaaggagcaa
     2101 catgtacatg gctaatgaag aaacagtagg tttgagaatc atagaagaag cattcaaatt
     2161 accaatggtc aaagttgatc gtcgagcatt tttgattcaa acatttcaag ggaatgtttc
     2221 tgatattgaa ggactcatag aaaagggtcc acaagcattt ttatccaaag aaatgctaga
     2281 tcgtgaagca caaaaatgta ttcaatcgat taccttgaaa tcaagtgcgg tatcgttagc
     2341 aactggactg ccaggaggta tcgctatggc agcaactatt ccagcggatc tccttcagtt
     2401 ctttggttat tcattacgat tagcacagga aatttcgtat atttatggat ttaaggattt
     2461 aatgggagag actggagagt tcagtgaaga agcaaagaat acgctgatat tgtatttggg
     2521 aattatgctt ggagtaactt ctgcaggttc tgccgtacga gcgatgtctg gaaagctgtc
     2581 tacacaagca ttgaaaaaga ttcctcaaaa agcattgaca caaacgctgt attatccagt
     2641 aatcaaacga gtactaggta tttttggtac aaagttaact aaaaatacct ttgcgaaagg
     2701 tatttctaaa gcagtaccag ttgtgggagg cgttgtttca ggaggcatca attacttgtc
     2761 tatgaaacca atggcaacga agcttcaaac agagttaggg aaaaatgtag cgtatacaga
     2821 aaaaaatctc caacaagatt tagatatttt agaaggtgaa tttgaagaag tatcttctga
     2881 tatcgtgtct tctgatacag ttctttctca attagaaaga ctttctcact tgttggaaac
     2941 gaatatgatt acagaagaag agtttcaaca attgaagcaa gagctgttaa agaaataatt
     3001 atttagaaaa agcaaccact tatacgggtg gttctttttc ttgaatgaat tcaaatttat
     3061 tattaggaga agaaaaagga tgaaagcaaa acgattattc ctattaggca cgattctttt
     3121 acttggagga tgttctactg tctctcaaaa tgaacaaaag aaacaagtgg attcaacaac
     3181 ttatgaaact gaaacaacaa gaaaggaatc ttctacaaaa gaaatagaaa caactactca
     3241 agaaactaaa acaacagagt cttctagtaa ggcaacgagt tcagatacga gtgattttca
     3301 aataaaacaa gaattatggg atacaaataa agcatctcaa ttagcaactt ttgtcccaca
     3361 atggggaaag acattaggtc aagaatataa aagctacaat cctcagaaca atgtaagtct
     3421 gtatggtacg cctttaccaa gtgcagttat tgatggaaat tggaaaatgg caatcaatga
     3481 agcaccagtt actgtacaat ggtcagaaga tggaactgga aatgcaggat ttaatttagt
     3541 agcagtttat tctgatgtag aaactggaga atatttaggg cagcatgttt actttttcgg
     3601 ttttcaaaat ggacaaccta aagtttattt aacgcaacaa aatcaaggta atgaaaataa
     3661 ttatctgtac tttaacgaaa ctcaaaatca acagctaaaa caaggattta cagatatcat
     3721 taatggacaa actccccaaa caccagtagt agaagaatct actcaacaag caacaactac
     3781 aacgacacaa tctacaagta gttcaggaat ttcaaattgg gaagctgcta aagaaactgt
     3841 actaaataat aaagcgttat ggatttatga gtctccagat aaaaatgaag caaatactaa
     3901 tattattgaa tccacccaat atagaaaaga tttaatgcaa gatgaaaatg gagaatttta
     3961 tccagttgat atttctactg atgatattgg aaatacggta atgaatggag gaaaacaatt
     4021 ccgtgtttat acagatggaa gaat
//
LOCUS       DAYYKN010000188         4034 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000188 DAYYKN010000000
VERSION     DAYYKN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.191"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..437
                     /locus_tag="ACHBB7_002720"
     CDS             3..437
                     /locus_tag="ACHBB7_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210607.1"
                     /translation="MIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGND
                     HSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAV
                     LLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(498..863)
                     /locus_tag="ACHBB7_002721"
     CDS             complement(498..863)
                     /locus_tag="ACHBB7_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM9210608.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(939..1184)
                     /locus_tag="ACHBB7_002722"
     CDS             complement(939..1184)
                     /locus_tag="ACHBB7_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9210609.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1254..1508)
                     /locus_tag="ACHBB7_002723"
     CDS             complement(1254..1508)
                     /locus_tag="ACHBB7_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210610.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1672..1995)
                     /locus_tag="ACHBB7_002724"
     CDS             complement(1672..1995)
                     /locus_tag="ACHBB7_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210611.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2182..2255
                     /locus_tag="ACHBB7_002725"
     tRNA            2182..2255
                     /locus_tag="ACHBB7_002725"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2216..2218,aa:Ile,seq:cat)
     gene            complement(2640..3137)
                     /locus_tag="ACHBB7_002726"
     CDS             complement(2640..3137)
                     /locus_tag="ACHBB7_002726"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM9210612.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3177..3986)
                     /locus_tag="ACHBB7_002727"
     CDS             complement(3177..3986)
                     /locus_tag="ACHBB7_002727"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9210613.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT         1346 a          718 c          708 g         1262 t
ORIGIN      
        1 ttatgataaa aaaactattt ttactatcaa tattaacgct agcaatggca ggttgttctg
       61 actctggctt agcaactgaa ctgatcacaa aatcaactga agccacgaca gaggagttaa
      121 aatgtggaaa cgatcacagt gactacagag gcttaaatcg acgatgcatg ggaatattag
      181 gcagaagcaa ttgtaagcat cgatgtagta atcgaagaag gtgtagaaga agcaacagaa
      241 aaatcaaaag aaagatcaaa agaagcaatc tgtgctgtgc atcgtataca gacgtcgaag
      301 atgcggtact tttaaaccac gtcaggagat gttcatttat gtctagggaa gttggcgatg
      361 aaccaaaact agagtacgct tttagttttg cacgtgagga tgaagataac cctaaaactt
      421 acgacatttt agcttgatct aagatggtta aagcttttta atgaagtaag aggagataga
      481 aggcttttct agttatatta ggtaagataa attccttttt gctccaaatt tgctaaacat
      541 tctgctagat aaacttcatc gtgttccgga taaatcggca cgttgcgctc tacagtaggt
      601 aaatcaaaaa atgcttcgca tgatttgcca cgatacatag gatttttcca ctctggtgct
      661 gggctgtaac cacgtttttc catttcatcc atgacaagct gatgatattg gaataatcta
      721 taaggagaat agtgaaaaac ataatccaca gtagcatgtt tctttcccca gccatttcct
      781 cttaatgcac agcattcccg atgttgtcct aaaagctgtt gacgaggaag ctttgaaatc
      841 aatttttcat gccataatcg catagaaatg tctcctttat atatagattg ttaaccttag
      901 acgttaaatg aaaaaaccag caccaaggct ggaaaaaatc acttcagttt gcttgctgtt
      961 tctgagcgac caattaggta atcaacggaa atatcgtaaa agtcagcgat atagattaat
     1021 tcaatgatgc ttggaatttc ttctccttct tcgtacttac gatatttgaa taaaggaata
     1081 cttaacgcgc tagaaacagc tacttgagat aaagatcggc cagttcttaa gaaattcaga
     1141 cgacaacgta aagtatccag taaaatagct tcttcgatac tcatttaatc acctttcatt
     1201 atagtagaat agttataatg actatagaaa gctacacatg caatcaatag tactcaatgt
     1261 tttttattta aacgtatcaa ataaagaatg acgatcaata aagacaaaac cagtcctgat
     1321 aattcttcta taaaaacact atcgggttca aagtagttgt aattaaaaac tttccacata
     1381 aagtagatag ccggaagagt caaaatagct aaataaaacc gttctttgtt ccaattcata
     1441 agataaacaa tacttgcgac cagtgcgacc gcaggcaccc aaaacaaaac tctcaccaac
     1501 gtaatcatgt aacggactcc tcaacgattc atataataaa agataacatg ttattgcata
     1561 catcacaaat gaaatgctag gaaaataaaa gaagaataga tcaatgaaaa atgaaaaagc
     1621 agaacagttt tctgctgata agcaggaact gttccgctga atgtgtctta tctatcgaga
     1681 aaaaatcaat ggattgatat ctaagtaatt caatacatat aaaaagagga cgatcgcatt
     1741 cactaaaaag gaaagaatga tgatcccata gcgaatggaa acatatttaa caaatacttt
     1801 tgtttgaata cgggcatatc tttttccttt atagctgagc atcatgacaa gaagcaaaag
     1861 ggtaatcaca ccttgaaaaa tcattccaat aaacagaatt cctgaggttt gataatttaa
     1921 ttggagaaaa taagctgctg ccgcaactaa atcgaataaa atcgcaataa atggcaaaat
     1981 aatcactcct aacattgaga ataaataaaa gaataacatt ttttctaaaa taagcaaata
     2041 aaaattaaag taacaaaata gttatgacac aagtaaaaga aataagaata acaatattat
     2101 tttacttata ttttgaaact tgtgagagtt tgtcattcgt gctatagttg aaaagtaaca
     2161 aaaataaatt ttatttatga tgtaccttta gctcagttgg ttagagcaga cggctcataa
     2221 ccgtccggtc gtaggttcga gtcctacaag gtacataaga ggttgtgaaa aagctgccct
     2281 gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttac ttggctgttc
     2341 ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg aaatcagcgt
     2401 tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt gccgtttgtt
     2461 aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg tgagcctgcc
     2521 gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac gtaggactaa
     2581 aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga tagatatttt
     2641 tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga ttctccgtgt
     2701 tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa agcactctgc
     2761 aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac ttcgatctct
     2821 ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt ttcaggtgga
     2881 atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag aagagggatc
     2941 aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat atcccgtgtg
     3001 aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat acgttctgcc
     3061 aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg attttgcata
     3121 tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta ttctttttaa
     3181 tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt tacttggttt
     3241 gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc gctgtaattc
     3301 aacatagcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga ttcgattcca
     3361 aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg cgtgatttct
     3421 aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc tctttgtagc
     3481 ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct aagagaagga
     3541 tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa atgttcataa
     3601 taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc ttgggtttct
     3661 cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt tctcagtgta
     3721 tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt catttcagca
     3781 atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga aaaaatatct
     3841 ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt ttcatcaaat
     3901 ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag caaggtaccg
     3961 tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac atatctattt
     4021 tcctgttacc ttaa
//
LOCUS       DAYYKN010000189         3925 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000189 DAYYKN010000000
VERSION     DAYYKN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.192"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..2146
                     /locus_tag="ACHBB7_002728"
     CDS             152..2146
                     /locus_tag="ACHBB7_002728"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9210614.1"
                     /translation="MVLKQGKQDYQIIGYLEDDLNKIKINSDDVMYFFMTPLSDFPSS
                     VIDNVEGIQIYSNTLTKFGFIDDINESHSTRAQSLKEFLDPYIIVCTPKIKRNHEQAR
                     NYYQGDDLELIPKPKSEVFKNLIKENESLVPIPVFSINNSDSVIKDETSFENQLLNGK
                     LLGKIINFSKDNEDYPLNVFWENTDGSRVLFGEIIGQNNSMYGVAYRIEKDNFYKKFI
                     DEGSKNYVGYDDFINQTKDIIFVPYLLLQKIIETKKDINEGIENISDSKISPVSSIIT
                     EQTQIDISELEDKHAIQQNDHFSEHNEFIERFNQLTIRSKLHYSKRDLWNFHTAMLGD
                     SLVVLAGLSGTGKSQLVTSYAKALQLSDTQIKFISVRPFWEDDSDLLGYADTVNSVYR
                     PGDSGLIDALLEASRHKENIYMVCFDEMNLARVEHYFSQFLSVLEMEPNTRKIKLYNE
                     DLENKLYNSSTYPSTIEVGQNILFVGTINTDESTHQFSDKVLDRSNIISLELVPFHEI
                     SEVNDYIQSDQKKRNQLKYDDFKNFKNSNPTNGLTQKEKEMFWKLHLAINSKDKNIGI
                     GWRILKQIDEYLKNLPVQNDLSRKEAIDIQLVQRVLSKIRGSDEQLSELVGKWNENDL
                     KQVGEFEQIINEYPESSDFKYTKKILLQKARELNLHGFTL"
     gene            2130..3920
                     /locus_tag="ACHBB7_002729"
     CDS             2130..3920
                     /locus_tag="ACHBB7_002729"
                     /inference="COORDINATES: protein motif:HMM:NF016309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HFM9210615.1"
                     /translation="MDLPFSLKLNFGYKSEEQEYELLFFQDEASLLEYTPVKVKEFSN
                     LSFTFNSIDKHAKLYIPELDDYLIENQVKEENGDICYIPSDDNIIIYNRDQGKTVPLI
                     PGYYTLYVKIGETIFYSRFEIILKDLGTPEWEKMKIEIEKMVGGLARTFIPKKTSRRE
                     ITTSNYLMNDQLFQRILEFENNIPQAIVSLESLKKEAKYRIDKRYNWKPIGSKNLVDQ
                     KTISQLQIHPEKRGLLYTPSRIIKYDIIENQWIKFILSYFKNFSLLADRYLEKILQGI
                     IEEKKREDKFTSAYSQSVQNYNTNSYNRKIENLKNNQQKIKHFYAYILEFLDTPVMKN
                     ISSRRPINVPKSLVMNPKYNTIYKMYLKSRNNRNTLNFSNRYQYYWKSTDVLYEIWCF
                     IKIIRSLSLQGYTPISGWIFENDILSQELPFLHEDTTVVLSKNDIRINLVYNNSMEKE
                     YFSNTLDIPVKTSSKRNKPDIRLDIILQDKYYIGSLIFEVKYKKLDNILDNDKGRQRQ
                     QLMAYKQNTMSSILAFPEILTRSLQAVSAVFALYPSNEGRKKTAPKYYEKEGIFFHLL
                     NPSSDEKELSVKIQTSIEERISLFNQQSRN"
BASE COUNT         1590 a          526 c          576 g         1233 t
ORIGIN      
        1 ataattgaag aaaatgatat taatgaatta tggattacaa cctttcaatt aattcccaga
       61 atgaaaaaaa agattataca aagtttaaaa aataaaataa aaattcaaga attaaatgat
      121 tttactatgc ttatgaagga ggaaaatata aatggtttta aaacaaggta aacaagatta
      181 ccaaattatt ggttatttgg aggatgattt aaataaaatt aaaataaact cggatgatgt
      241 aatgtacttt tttatgactc ctctttcaga tttccccagt agtgtgattg ataatgtaga
      301 gggtatacaa atatatagta atacgcttac taaatttggg tttattgatg atataaacga
      361 atctcatagt actcgtgccc aatcacttaa agaattttta gatccttata ttatcgtttg
      421 tacaccaaaa attaaaagaa atcatgaaca agcaagaaat tactatcaag gtgatgattt
      481 agaattaatc cccaaaccaa agtctgaagt ttttaaaaat ttaataaaag agaatgaatc
      541 attagtacct ataccggtct tttctataaa taacagcgat tctgtaatca aagacgagac
      601 aagttttgaa aaccaattac taaacggcaa actactcgga aaaattataa atttctcaaa
      661 agacaatgaa gattaccctt taaatgtttt ttgggagaac actgatggat caagagtttt
      721 atttggtgaa ataatcggac aaaacaattc aatgtacggt gtagcatata ggatagaaaa
      781 agataatttt tacaaaaagt ttattgatga aggtagtaaa aactatgtag gttacgatga
      841 ttttataaat caaacaaaag atataatttt tgtgccctat ctattacttc aaaaaattat
      901 agaaactaaa aaagatatta acgaaggcat agaaaatatc tctgattcca aaatatctcc
      961 agtaagttca attattactg aacaaacgca aatagatata tctgagttag aagataaaca
     1021 cgctattcaa caaaatgatc acttctctga acataatgaa tttatagaaa gatttaatca
     1081 gctcacaatt cgtagtaaat tacattactc aaaacgagac ttatggaatt ttcatactgc
     1141 tatgttgggt gatagtttgg tggtattagc tggactaagt ggtaccggta aatcacaatt
     1201 agtaacatcc tatgcaaaag cacttcaatt atcagataca caaataaagt ttatttctgt
     1261 gagacccttt tgggaggatg attctgattt attgggctat gctgatactg taaattctgt
     1321 ctatcgtcca ggagatagcg gtctaattga tgcactatta gaagcatcaa gacataaaga
     1381 aaatatttat atggtttgtt ttgatgagat gaatttagct cgggttgaac attatttctc
     1441 ccaatttctt tcagttttag aaatggagcc aaatactagg aaaatcaaac tgtataacga
     1501 agatctagaa aataaattat ataactcttc aacctatcct tctacaattg aagtaggaca
     1561 aaacatatta tttgtaggaa caataaatac agatgaatct actcatcaat tctcagataa
     1621 agttttggat agatcaaaca ttatttcact tgagttagta ccattccatg aaattagtga
     1681 agtaaatgac tatattcaat cagatcaaaa gaaacgtaat caacttaaat atgatgattt
     1741 caaaaatttt aaaaactcta accctactaa tggtttgacc cagaaagaaa aagaaatgtt
     1801 ttggaaactc catttagcaa ttaattctaa agacaaaaat atcggtatcg gttggagaat
     1861 attaaaacaa attgatgaat atttaaaaaa tcttccagtg caaaatgact tatcaagaaa
     1921 agaagctatt gatattcagc ttgtacagag agtattaagt aaaatcagag gttctgatga
     1981 acaattatca gaattagttg gtaaatggaa tgagaatgat ttaaaacaag tgggtgaatt
     2041 tgaacaaata attaatgaat atcctgagtc ttcagatttt aaatatacta aaaaaattct
     2101 tttgcaaaaa gctagggagt tgaatttaca tggatttacc ctttagtctg aaattaaact
     2161 ttggttataa aagtgaagaa caagaatatg aacttttatt ttttcaagat gaggcatcgt
     2221 tattagagta tacaccggtt aaggtaaaag aattttctaa tctaagcttt acatttaaca
     2281 gtatagacaa acacgcaaaa ctatatatac cagaattaga tgattatttg attgaaaatc
     2341 aagtaaaaga ggaaaatgga gatatttgct atattccaag tgatgataat ataatcattt
     2401 ataataggga tcaaggtaaa acagtacctc ttattccagg ttattacact ctttacgtta
     2461 aaataggtga aactatcttt tattctcgat ttgaaataat acttaaagat ttaggtacac
     2521 cagaatggga aaaaatgaaa atcgaaattg aaaaaatggt tggcggtctt gctagaactt
     2581 ttattcctaa aaaaacttct cgtcgtgaaa ttactacttc aaattatcta atgaacgatc
     2641 aactttttca acgaatatta gagtttgaga acaatatacc tcaagcaata gtttctcttg
     2701 aaagtttaaa aaaagaagca aaatatagaa ttgacaaacg atataactgg aaaccaattg
     2761 gaagcaaaaa tttagtggat caaaaaacta tttcacagtt gcaaatccat ccagaaaaac
     2821 gtgggctact atatacacct agccgcataa taaaatatga tattattgaa aatcaatgga
     2881 tcaaatttat cttatcttat tttaaaaatt tttcacttct agctgataga tatttagaga
     2941 aaatcctaca aggtattatt gaagagaaaa aaagagaaga taagtttact agcgcgtatt
     3001 ctcaaagtgt acaaaactac aatacaaata gctataatag aaagattgaa aatttgaaaa
     3061 acaatcagca aaagataaaa catttttatg cttatatcct tgaattttta gacacaccag
     3121 taatgaaaaa catttcatca agaagaccta taaatgttcc aaaatcactt gttatgaacc
     3181 ctaaatataa cactatatat aaaatgtatt taaaaagtag aaacaatcgt aatactttaa
     3241 atttttcaaa tagatatcag tattattgga aaagtacaga tgtactctat gaaatttggt
     3301 gctttataaa aattattaga tctctatccc tgcaaggcta cactccaatt tctggatgga
     3361 tttttgagaa tgatattctt tcacaagaat tgccgttttt acatgaagac actaccgtag
     3421 tattgagtaa aaatgatata cgtattaatc ttgtatataa taattccatg gaaaaagaat
     3481 atttctccaa tacactagat atcccagtaa aaacctcttc aaaacgaaat aaacctgata
     3541 ttagattaga cattatcctt caagataaat actatatcgg aagcttaata tttgaagtaa
     3601 aatataaaaa actagacaat atacttgata atgataaagg taggcaacgt caacaattaa
     3661 tggcttataa acaaaatact atgtcaagta ttctagcatt tcctgaaatt cttacacgtt
     3721 ctttgcaagc tgtctctgct gtctttgctt tatatccttc caatgaagga agaaaaaaaa
     3781 ctgcacctaa atattatgaa aaagaaggta ttttttttca cttattaaat ccttcatcag
     3841 atgaaaaaga gctttcagta aaaattcaaa caagtattga agaaagaatt tcattattta
     3901 atcaacaaag tagaaattaa aaaga
//
LOCUS       DAYYKN010000190         3868 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000190 DAYYKN010000000
VERSION     DAYYKN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.193"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..592)
                     /locus_tag="ACHBB7_002730"
     CDS             complement(50..592)
                     /locus_tag="ACHBB7_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM9210616.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            727..1116
                     /locus_tag="ACHBB7_002731"
     CDS             727..1116
                     /locus_tag="ACHBB7_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210617.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1120..1884
                     /locus_tag="ACHBB7_002732"
     CDS             1120..1884
                     /locus_tag="ACHBB7_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9210618.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2013..2960
                     /locus_tag="ACHBB7_002733"
     CDS             2013..2960
                     /locus_tag="ACHBB7_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9210619.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3090..3830
                     /locus_tag="ACHBB7_002734"
     CDS             3090..3830
                     /locus_tag="ACHBB7_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210620.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1282 a          659 c          793 g         1134 t
ORIGIN      
        1 taacatttaa aatttaattt agatattaga cagtttttaa gtttgtgagt tattttcctt
       61 gtcgttttct cactttttta gtccagaaac cacctgaaat atattttggc tcataagaaa
      121 tgataaaagc acgctcttcg atttctttga tcaggcgata cagttcacgt tcgctttttc
      181 taggagaaag tatttcgaga actagccgct caccttccag ccccattgca gaagtctgtg
      241 tcacaccata accgtgttct ctcagtatac ccggcaaact tttatcttct gcgctatttg
      301 ctggcaaaat cgttgtgacc atgatgtatc ctaatgccaa tttgtcttca atcaaaattc
      361 cgacactgat acctactgcg taacctaacg catagacaaa taaattcagt ggattatcta
      421 aacgatctaa aacaagggat aagccgacga tataaatcgt gatctcgacc atactgacaa
      481 gtggagcaat cagtcgatag cctttcattg tcaacatgaa acgtaacgtg ttcagagtga
      541 tataagcaaa attaatgagg aagatcatcc ccaacatttt tggatcaatc atttgtaaaa
      601 ctcctttcat ttatataaca acaatatcaa aaaaaatcgt aagtgaagaa aaattgaaac
      661 tttatacata caaaaaaatg ataaaatgat tataatagaa ttataaagat aggttaggtg
      721 attaaaatga aaacggtcaa tcgtgtgttt tgtgccagcc acgtgctaag tctccttctc
      781 agtatatact taggtgtcta ttcgattcaa cagccaattg aggtagagaa cattgttttt
      841 cagatgtcgc tgacagatgg tttaatttta agcgtttttt tctttttgat actgtttatt
      901 ggtaatattt tcggtagcgt tgccagtttt ttgaattttt ctatttatcc actcctttcg
      961 ctagcattag gagtggtggg actttgttct ctgtgcttat ttcccgagcc attcagtcct
     1021 gcatttatat tatttggtac actgaacctg ttccagacaa ctgttggagc atggttgcta
     1081 tggcgatcag gaaatttaat gaaaataggt gagtaacaat tgaatccaat tatattgaaa
     1141 cgttctgtcc atcatattcc agtgttagaa gttgttctga aagaattaag agaggaacgt
     1201 attcccgttg tcatctatta tcacggatgg cagacaagta aggagttagt tttgacgcaa
     1261 ggaagaaaaa ttgcgcagca gggcattcgt gtgatcctgc cagatgcgat gaatcatggg
     1321 gagcgcaaac agccggtttc ttctattcca tcctatacat tttgggatag tatctacgga
     1381 aatctgtttg aatttgacac acttatcaag catttagaaa aaagaggtct tctacatgat
     1441 cagcttgcag ttggcggggt ttcgatgggg ggaatgacaa catgcgcctt gttggctaaa
     1501 catcctcata tagtagcggg cgtgtgtttg atgggttccc ctgcaccttt ggaatatgga
     1561 aaagaaatcg taaggcgcgc atcggaatat aattatgtcg taccaaaaga ctattttgat
     1621 ttgatttcat gggttaagag ctatgatttg tcattgcagc cggaaaagtt agctggacgt
     1681 cctctgttct tttggcatgg acaagctgac gaaaaaatcc cttatataca aacggaacga
     1741 tttgtagaaa aaaatcggca taagtcttac ggaaaagaaa ttgtttttcg ttcctcaaaa
     1801 acacagcgac atatggtaca aataccacta atggaagaag cttccacttt tttagcaaaa
     1861 aatttgataa aaaagtataa ataattgaaa cggaaaatca ctcatgacga tagaggattt
     1921 tccgtttttt attttgggct ataataaact ctgttaggaa aatttggtat aattgatagt
     1981 aagaatgaca acatgttaga aaggaagaga gcatgcccac tttagcagat gttgcaaaac
     2041 gtgccaatgt ttcaaaaatg acggtctctc gagtcatcaa tcatccggaa caggtgactg
     2101 acgaattgaa agagttagtt ttctcagcaa tggctgaact ggattaccgt ccaaatattg
     2161 ctgcaaaagc acttgtcacg aatcggtcgc agatcatcaa attatttata ctggaagaaa
     2221 tcgacacaac tgagccctat tacatgaatt tgctgatggg aatcgctaaa cggatcggta
     2281 aagcgcatta ttctctccaa ttagttacaa atgatgcctt cgacgtcggt tcttgtgatg
     2341 gatacatcat cacaggtgtc agaaaaaaag acttcgattg gatcgaccgt ttggaaaaac
     2401 cagttgtctt gtttggggaa aattctgaag gatatgatta tgtagacagc gataatgaat
     2461 atggtacagc aaaagcaacg caatatgcct tagaaagaga ttatcaaacg attatttata
     2521 tcggtatcga agtggacgag ccttttgaaa tttcaaggga aactggttat ttatctgtga
     2581 tgaaggaagt tgatcttcct agtaaaatct tgcggtttcc aaatcattcg acagaagtag
     2641 aaagatatat gacagaaaac tgggcagatt ttacagataa aacttgtttt gtatgtagtt
     2701 cagatcggct agcactagga gcagtaagag ggattataaa tgctggagga cacttgcccg
     2761 aagaatttgg tgtgattgga tttgatgggg tatttttaga ccaaatcagt tcacctaaat
     2821 tgacaacgat caagcaagat attatacgta tgggtgaagt atgtggagaa atgttgttaa
     2881 aaaaaataga agaaaaaggg gcgagtcaag gttatcgtcg tttttttcct gaactagtta
     2941 tcagagagac aacgaattaa gtcttattac tatatatata tttatgaaat tcatcgctat
     3001 tttcttaaaa taatcagcgc attgctactg aaaacaatga atctgtgcta ttattgagaa
     3061 aagttccatt tttgagaagg atggtttgaa tgaaagaaat cagacgaaaa catttccgtt
     3121 ttcctgcgta cgatgatgag ttaggcgtaa aattaaccaa cgacaagaag cgagtattat
     3181 ttgacggaca agaagatctt ttgactgaac agaatcagtc cccgtttgaa cagatgaaag
     3241 aatttcattt cacgaacacg aacaccgaga aaatccgtcg caagcgtcgt gcaacaaatg
     3301 gattgaagtc agctccgcgt aaggaaaatt tggaaaagca taaaggaaaa ttaccggatt
     3361 ataccggcca ttaccaatca gaaatcacat cgacaggacg aaaaaaactt tttggtgatt
     3421 cctctcgttc tgtcagacaa agtgaaacaa ggacagagtc caagcatact acacgaaacg
     3481 catctgttga cacgaaggtg cgggagcgtt cttattttgt tcctaaatat gtacccgcat
     3541 cgattatccc agaccctaga gaaagcaaaa tttctgagga tcatttaatc gaatcgatga
     3601 aaaaaacgga tgactcttat ctgttgttcg atacagaacc gacgccgtat caagaaaaaa
     3661 gaaatggtga gccgtctgta cgaacattca atcgaacaaa aacgttagag gaaacaaagc
     3721 agtcagaaga aaaaaaatcg aaaaaaagcc atggtatctt agaacgctca ttacaaggat
     3781 tgatcgatga tcaaggaaac tctttgggag aaaatagcta ttttaaataa gtgatataca
     3841 aatttatatt cggctacagt tggaaagg
//
LOCUS       DAYYKN010000191         3836 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000191 DAYYKN010000000
VERSION     DAYYKN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.194"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHBB7_002735"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHBB7_002735"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM9210621.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACHBB7_002736"
     CDS             complement(2260..2511)
                     /locus_tag="ACHBB7_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM9210622.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACHBB7_002737"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACHBB7_002737"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM9210623.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACHBB7_002738"
     CDS             3461..3679
                     /locus_tag="ACHBB7_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210624.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1063 a          814 c          712 g         1247 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttct
//
LOCUS       DAYYKN010000192         3748 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000192 DAYYKN010000000
VERSION     DAYYKN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.195"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..331
                     /locus_tag="ACHBB7_002739"
     CDS             <1..331
                     /locus_tag="ACHBB7_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210625.1"
                     /translation="LLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSIS
                     LCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNF
                     YFYRNKL"
     gene            complement(457..1134)
                     /gene="nth"
                     /locus_tag="ACHBB7_002740"
     CDS             complement(457..1134)
                     /gene="nth"
                     /locus_tag="ACHBB7_002740"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM9210626.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1141..1839)
                     /locus_tag="ACHBB7_002741"
     CDS             complement(1141..1839)
                     /locus_tag="ACHBB7_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM9210627.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2107..3291
                     /locus_tag="ACHBB7_002742"
     CDS             2107..3291
                     /locus_tag="ACHBB7_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM9210628.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3297..>3748)
                     /locus_tag="ACHBB7_002743"
     CDS             complement(3297..>3748)
                     /locus_tag="ACHBB7_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9210629.1"
                     /translation="VEMDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIE
                     HIGKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQ
                     KIRNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          968 a          745 c          627 g         1408 t
ORIGIN      
        1 acttttaagc actactctta aaggaaaatt tatggaaaga agctctcatg caatgattct
       61 cactttttta tggttgttgt tcaccagaat ctatattttt ctgatgagcc tgaaaccgcc
      121 caaatccatt agtttatgtt tagttataaa tggtattttt ttattatcac ttatcatttt
      181 tattacacct ttagatagat acgtcactta tttatataat cctttggaat actggactta
      241 ttttataggt atattggaag gcattatttt ctacatcggg tattttccca ataaaaataa
      301 caacttttat ttttatcgca ataaattgta ataagttacc atcctagcta ctgagtatgt
      361 acatttactt tttcatgcaa aaaagataaa cgaaacagct tttctccata ataacaagat
      421 aattcataca tttatttgca attaaagaaa ccacgtctac aggcgtggtt tcttttttag
      481 cgccttttct tttagacgca tccgattttt cccatcttga catattgata gcaaaggaca
      541 tacctcgcac ttgggatttc ttgccgtgca atgatatcta ccaaaaaaaa tcagtgtatg
      601 atgtgtcttt acccataatt cttgtggtac tttccgcatc agtgtttctt ctacttccat
      661 gaccgtagca tctagtttgc agatcctcag gcgttttgag acacgttcca cgtgggtatc
      721 tactgcgatt gctggtatcc cgaatgcatc ccccaatacg acatttgctg tctttctgcc
      781 tacaccagga agagacatca attcttctct tgacgttggc acttgcccat cgaaacgttc
      841 aatcagctgc tgtgcacacg ctttgatgtt ttttgcttta tttcgataca aaccaatcgt
      901 ttttatttta agaatgattt catcgattga agcttctgct aacgcatcgg gtgtcggaaa
      961 agaggcaaac aaatctggcg ttgctttatt cactgataca tctgtcgcct gggcacttaa
     1021 gatcactgca atcaataact caaatggatt attgtgcttg agttctccat gtgcttcggg
     1081 aaacattcca tacatcgttt ctagagcttc cattgttttt tgtttgctta acatattccg
     1141 ctactcctcc aaccaatttt tcagtgagac tttcggcaat tcttcttgtt gagctgactg
     1201 ttctacttct ctttgaagaa gctgctgttt tcttcgtttt tggtcttctt ccacttgttg
     1261 ttttgtacgt agattttttc tttcccacga tagcaaaatg cggtcaacgt aattaaaact
     1321 ataagcctga tttaatacgg cttcccgcag tgctagcttc aaaatttccg gctgataatg
     1381 gtcttcttct agccattgtc cgatccgctg gaactcgatc gaagaaagcg ggcgtccaaa
     1441 ttcttgttca aataattgat acactgactg gatttctttt tcttgtgttt tttcttcgta
     1501 ttttttttct tgtgttttta aatactcacc taatagatca tagatggggt aaagattata
     1561 tcgatctgct tttttaccgt tgacaatcgt cgtttctatc ttgataaatc catttgttac
     1621 gagacgatcc agtatttgaa aaatctgatc ttgtttgatt cccatatcca tagcaatcat
     1681 ctgcaaatca gggaaagtat ctccttcaag ctgtgccata tgcaactgta gaacaaataa
     1741 aaattcttca gattgtagac cgatcctatg ataattttta agtaaaagat tagatatgac
     1801 tgtttgtcca gcgcttaagt aattttctaa attcaacatg atttctcctc cgctcaatca
     1861 cagtatacgt aaaaaaaaca tgggaagcaa gaacaggttt cttttctatt tacttcactg
     1921 atcggtcaga tttacaatgt cgtctacttg atttttgttt gttcatgttt ttgatgcgta
     1981 tcttcacttt tcggaataaa taaattaagt agatttcgct gttccttaga atgttgacaa
     2041 ggaaacaaaa ttactataca ctatttttgt ttccaagtta acattattgc ataaaggagt
     2101 ttttacatgt acagtttcac aaataaacat tctaaagttt ttggatacgt ctctgtctta
     2161 ttcactggat taggatttac tatcgtcagt cctgtacttc cgtttttaac gctgccttat
     2221 tctcactcca tacatcagca agctttctat atcactttgc tcatgtctgt ttatgctcta
     2281 gctgccttct tatctgcccc cattctcggt agcttaagtg atcattttgg gcgtcatccg
     2341 atccttatta tcagtttatt aggatcaagt atcggatatt tgctttttgg tttaggaaat
     2401 tcgatatgga tgttgttcct tggtcgtatc attgaaggat tgacggcagg agaaatctcc
     2461 accttatatg cttactttgc cgacatcacg gaacctaatg aaagaaccaa agtttttgga
     2521 tggatgggtg ctttagtcgg tataggaaca acgctgggtc ctattatagg gggtctatta
     2581 gctgagttgg ggaattctgt tcctatattt attggagcac tctttacttt cttaaatgcc
     2641 gtttatggct acacatttat gctagaaagc cttccaataa aaaaacgttc agtagattta
     2701 agtttctccc atgtaagacc ttttcaccaa ttacaacaat tattcaagat ttcatccgtt
     2761 attcctttat tgacagcagg ttttgctgtt tggttggcag ctggctctct tcaatctatc
     2821 ttttctcaat tttcgattga cgcctttcag tggaaggctg ggttggttgg tttaagcttc
     2881 tctttgattg gaatcttgga tactgtctcg caattgttca ttatgcctcg attgctcaaa
     2941 aaattttcag aacagcagat cacaagaata gggatgttca gtgaaatcct cgcctatcta
     3001 ttcatcacat tatctggaat tcttctactg ccgattttgt ttttatttgg gattatctgt
     3061 tatggtttcg gtgattctat ttttactcct gtattcaatg gccaactatc caattctgtc
     3121 tcagataacc agcaaggtct tgtaatgggc ggtacccaaa gtatacaatc tctatcgcgc
     3181 gtcatcggcc cgctaatcgc aggacagctt tatgctgcag caccatttct tccgacggta
     3241 ttcggatttt tgcttatgat ttttgcctat tatcactaca ataaaaaata gtagcttcat
     3301 ttgctacttc gtataaattc tctgtatcga ccgataaaac gaaatactgt gtcaaattct
     3361 tcttttgaca tattttcaaa tacagtagca tctcgctgta cccatttttg atggagttgc
     3421 tcgtgtctat tccttatttt ttgtccgatt ggcgtcaatt gaaaatagat ttctttttta
     3481 ttctcttcgc tttgataagt gtttatgatc cctctagcta tcagtttttt tgttagctta
     3541 ctgatggctc ctctagtcat gtaactagca ttcgctaatt ttgtcacgtt aggatcaggg
     3601 attttcccga tatgttcaat cacttctagt tcatttagag taaatcctgc aaattctttt
     3661 tccatttctg gtttattacg atgaataatc tcattagcta aatccagata ttcttctatt
     3721 agcagttgtc ttttatccat ttctacac
//
LOCUS       DAYYKN010000193         3719 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000193 DAYYKN010000000
VERSION     DAYYKN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.196"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..354)
                     /locus_tag="ACHBB7_002744"
     CDS             complement(<1..354)
                     /locus_tag="ACHBB7_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210630.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(614..1162)
                     /locus_tag="ACHBB7_002745"
     CDS             complement(614..1162)
                     /locus_tag="ACHBB7_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210631.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="ACHBB7_002746"
     CDS             complement(1163..2020)
                     /locus_tag="ACHBB7_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM9210632.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="ACHBB7_002747"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="ACHBB7_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="ACHBB7_002748"
     CDS             complement(2582..2911)
                     /locus_tag="ACHBB7_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9210633.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2988..3260)
                     /locus_tag="ACHBB7_002749"
     CDS             complement(2988..3260)
                     /locus_tag="ACHBB7_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9210634.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3260..3523)
                     /locus_tag="ACHBB7_002750"
     CDS             complement(3260..3523)
                     /locus_tag="ACHBB7_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9210635.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1092 a          754 c          473 g         1400 t
ORIGIN      
        1 aaatgttctt actgcctctt ccacactaat ttcttcgttc aatttcacta ataatacttg
       61 atctcgctta ttataatcgc tttcatttat cctaagagtc aaatcttttt ccaccataac
      121 ttttacaccc aataaatttt gcggtttatt tttctctgtt gaaattcttt gtgtagcctt
      181 taaactgaca attagctcat tgggtacata ctctatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca
     3061 acccggtgtt gtctattcaa tcttctagaa tacctacctt catgtgtagg ttttaacttc
     3121 tcaaatgatt gggtagggag atagggatct tcctttaaag tctgaataac cttctcaaat
     3181 tgaggtttca aattagactg tttgattttc tttaaatcag ctttagccga gtttttaatc
     3241 atcacagaat agttattcat tataagttat cccaatcaat ttcatcaaca ttagtaaatc
     3301 cactatcatc tttttctcgc ttacgaacaa cacccaaagt gcctatttgc tcaagtaata
     3361 gcgtttcttt gattgctttc caatcatcca aaccaataag caccgcatta ttttcactgc
     3421 gctcactaat gatttcaatt tccgtatgat tctcatttac atctttcaac aacttataaa
     3481 aatttttccg tgctgagcta ggatttaaga ctgtttttgc catatctact ccccctctaa
     3541 tttatatctc cattctaacg tacttaatta agtacgtaaa ttcgattgtt ttgtttaaat
     3601 tggagtacca aaacagcatt gtaaagtgtt atccgtttcc tttatacccg tggtgctagt
     3661 taattttttc ctagaggaaa atgtctaaac atgttggtaa atcaacaaaa aaacgcata
//
LOCUS       DAYYKN010000194         3678 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000194 DAYYKN010000000
VERSION     DAYYKN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.197"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..251)
                     /locus_tag="ACHBB7_002751"
     CDS             complement(87..251)
                     /locus_tag="ACHBB7_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210636.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            546..1874
                     /locus_tag="ACHBB7_002752"
     CDS             546..1874
                     /locus_tag="ACHBB7_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM9210637.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2076..2909
                     /locus_tag="ACHBB7_002753"
     CDS             2076..2909
                     /locus_tag="ACHBB7_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM9210638.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2925..3662
                     /locus_tag="ACHBB7_002754"
     CDS             2925..3662
                     /locus_tag="ACHBB7_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM9210639.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
BASE COUNT         1228 a          577 c          767 g         1106 t
ORIGIN      
        1 tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac
       61 gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa
      121 taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc
      181 ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc
      241 aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt
      301 attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc
      361 attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac
      421 atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga
      481 gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg
      541 attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa
      601 atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg
      661 aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga
      721 aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc
      781 gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt
      841 ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa
      901 aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg
      961 gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga
     1021 tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac
     1081 tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta
     1141 ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag
     1201 gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg
     1261 accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt
     1321 atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag
     1381 agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa
     1441 ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg
     1501 atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg
     1561 atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa
     1621 gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa
     1681 aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga
     1741 gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga
     1801 aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg
     1861 gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg
     1921 cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta
     1981 taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac
     2041 gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg
     2101 atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc
     2161 gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat
     2221 tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata
     2281 cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg
     2341 gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc
     2401 cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg
     2461 aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag
     2521 aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg
     2581 tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga
     2641 gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt
     2701 atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat
     2761 ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga
     2821 tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc
     2881 aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata
     2941 ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt
     3001 cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca
     3061 gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta
     3121 atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata
     3181 agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa
     3241 tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag
     3301 agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg
     3361 aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta
     3421 atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact
     3481 ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg
     3541 ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac
     3601 atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat
     3661 aaaaaagagg ttgtgaca
//
LOCUS       DAYYKN010000195         3655 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000195 DAYYKN010000000
VERSION     DAYYKN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.198"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..1773
                     /locus_tag="ACHBB7_002755"
     CDS             7..1773
                     /locus_tag="ACHBB7_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM9210640.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            1796..3604
                     /gene="uidA"
                     /locus_tag="ACHBB7_002756"
     CDS             1796..3604
                     /gene="uidA"
                     /locus_tag="ACHBB7_002756"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM9210641.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT         1321 a          458 c          744 g         1132 t
ORIGIN      
        1 aagaaaatga ctataaaagt agatttacga aaaaaaccat ataatttaga tgaagatgct
       61 atttcttgga ttgaagagac gattgattct atgactttgg aagaaaaaat aggtcaattg
      121 tttgttaata tgggatctag tcgtgataaa aattacttga gtaatatggt caatcagtac
      181 catattggag ctgtacgata taatccagga actgctgatg aggtatatga tcaaaactat
      241 atcttacaaa cagaaagtaa aattcctcta ttaattgctg ctaatacgga agcaggtggt
      301 aacggagctt gtacagatgg tactgaagtt ggtgtagaag taaaaattgg tgcaacaaat
      361 gatgcgaaat atgcttatga attagggaga gtttctggga ttgaagcatc ggctattgga
      421 tgtaactgga gttttgctcc tattgtagat attaatagaa attggagaaa tcctatcatt
      481 tctagtcgat cttttggaca aaaccctgat aaagttttag aacttagttt agcttatatg
      541 aaaggtattc aggagagtgg aattttgcct gcagctaaac atttccctgg tgacggtatt
      601 gacgaacgag atcagcatct atcttttagt attaatagtt taggtacgga agaatgggat
      661 aattcttttg gaaaagtata ctcaggctta atagaagctg gattaccttc aattatggca
      721 ggtcatatcc atatgccagc ttattcaaaa cattttaatc ctgatctgaa agatgaagat
      781 ttacttcctg caactttatc aaaagaatta atcactgact tacttagagg aaaactcgga
      841 tttaatggat tagtagttac tgatgctagt catatgttag gaatgactgg ggcaatgaaa
      901 cgatcagaat tattgcccac ttctattgca gctggatgtg atctatttct attttttaat
      961 gatccagatg aagactttaa ttatatgatg gaaggctatc gtaaaggaat tatcactgat
     1021 gatcggttat tagatgcttt aaaaagaatt ttagggttaa aagctaaatt gggattacac
     1081 aaaaaagcaa aagaacaaat tttattacct aagcaagaag ctctggaaaa aattggttta
     1141 attgaaaata aatcaatttt taaagaggtg gctgaccaag caattacatt agttaaagat
     1201 aaacaagata tttggcctat tgtaccagaa aaatataaaa ggattttact agtagatgta
     1261 catggggtaa aaggtgggtt tggtgaagta gttgctggaa atagacctca agccattgat
     1321 ttattgaaac agattttgtc agcaaaagga tttgaagttg aaaaatacat ttcacctgaa
     1381 gaagaattga aaggattatc gcaagaagaa aaaattaaaa atatagctgg aatatatgct
     1441 gctaagagac caattaaaaa tttaactaaa aaatatgact tgatcattaa tatagctaat
     1501 gttaatccag gcacagttca acgaattgtc tggccagcaa gtaaaggaac tccggatata
     1561 ccgttttaca ttcatgaaat tccaacaatt tttgtatctg tacagtgtcc gtttcatttg
     1621 gctgatgtac ctcaagtaaa gacttatatt aatacttatg atggaaaaca agaaacttta
     1681 gaaatattgg tggacaaact aatgggtctt tctgaattta agggagtaag tccaatagat
     1741 gcttattgtg gtttaaagga tacatttatt taatatttag ataggagtgg tttatatgtt
     1801 gtatcccatt atgacagaga cgaggcaagt aattgactta agcggtctct ggagatttaa
     1861 attagataca aatgatgact tacaggaaga acttgcttta gaaccaataa atgaaaaaga
     1921 gagttatccg atttcagttc cctcttctta taatgattta tttgaaacag agaacattag
     1981 aaatcatgtt ggatgggtgt ggtatgaaaa agaagtgact cttcctaaaa gattattaga
     2041 agaaagatta gttcttcgtt ttggatctgc aacacatgaa gcaaaagttt ttttgaatgg
     2101 aaagtttatt actcaacaca aaggtgggtt tacaccattt gagtctgaaa ttaatcggtt
     2161 tgtaaaagac gggaataatc gaattacagt tgctgtaaac aatattattg acgatagtac
     2221 tttacctgtg gggattatga aggaaacagt acaggaaaat ggggatataa aaaaggaaaa
     2281 cttagttaat tttgatttct tcaattatgc aggtattcat agacctgtta agttatatac
     2341 aactccgaaa gactatataa gtgatattac tattaataca agaataactg atttggatgc
     2401 aagtgtagaa ttcaatatcg atacagaatg gaccggaaaa atcttaatag cgatatatga
     2461 cgaagaggct caacttgtag ctgaagctga aggaaagaaa ggaatgattc agcttaagaa
     2521 tccaattttt tgggaacctc tagacgctta tttatatagt atgaaaatta gtttaattga
     2581 tgataataaa gtgatcgata cctatacaga agaatttggt attcgcagtg ttcaggttac
     2641 caatggcaaa tttttgatta ataataaacc tttttatttt aaaggatttg gtaagcatga
     2701 ggattcgtat gttaatggac gtggtttgaa tgaggctgta aatattaaag attttaattt
     2761 aatgaaatgg ataggtgcta attcttttag aacatcacat tatccttact cagaagaaat
     2821 tatgcgatta gcagatcgag aaggtattgt agttattgat gaagtacctg cagtagggtt
     2881 acatttaaat ttcatggcca ctttattaga tgataatgtg gaaaaacata atacgtggaa
     2941 agagatacga acagaaaatt ctcataaaac ggtgatcaag gaattgatag aacgcgacaa
     3001 gaaccaccca agtgttgtaa tgtggtctat tgcaaatgaa ccagattcgg attccgaagg
     3061 tgctaaagaa tattttgaac ctttagttga attaactaaa gaacttgatc cgcagaaaag
     3121 acctgtaaca atcgttactt atctaaaatc aacgccagat gtttgtaagg taggagatat
     3181 tgtcgatgtt ttatgtttaa atcgttatta tggttggtat gtttcgggag gggatctggc
     3241 acatgcaaaa tatctgttga aaaaagagct agatggatgg atggaaagat gtcctgatac
     3301 gccaataatg tttacagaat atggagcaga tacagtagct ggatttcatg atacagtacc
     3361 aacaatgttt acagaagagt atcaagtggc ttattacgaa gcaaatcacg aagtaataga
     3421 tacatgtgaa aattttgttg gtgaacaagt gtggaatttt gcagattttg caacaagtca
     3481 agggatttta agagtgcaag gaaataagaa aggaatcttt actagagata gaaaacctaa
     3541 aatgattgct tactatctac agaaacgatg gtcatcaata cccaatttca gttataaaaa
     3601 ataatttgga ttttttaaga atgttcaaaa aacgattagc ctagcctaat cgttt
//
LOCUS       DAYYKN010000196         3529 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000196 DAYYKN010000000
VERSION     DAYYKN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.199"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(267..971)
                     /locus_tag="ACHBB7_002757"
     CDS             complement(267..971)
                     /locus_tag="ACHBB7_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210642.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1158..1403)
                     /locus_tag="ACHBB7_002758"
     CDS             complement(1158..1403)
                     /locus_tag="ACHBB7_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210643.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1420..2271)
                     /locus_tag="ACHBB7_002759"
     CDS             complement(1420..2271)
                     /locus_tag="ACHBB7_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9210644.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2437..3441
                     /locus_tag="ACHBB7_002760"
     CDS             2437..3441
                     /locus_tag="ACHBB7_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM9210645.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1092 a          603 c          683 g         1151 t
ORIGIN      
        1 cttttttctc tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca
       61 tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg
      121 taggatcata ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg
      181 aatttctttt tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc
      241 ccagcctttt atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt
      301 tcgatcaaag gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata
      361 acgaactcat tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt
      421 ttttcattaa taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca
      481 acggcttttg aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt
      541 tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct
      601 gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc
      661 cagtaagaca cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct
      721 cctgaacgaa ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt
      781 gtatcttcat ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca
      841 agcatgactt gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat
      901 aattgtcctc ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt
      961 tttttcttca tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc
     1021 aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg
     1081 cgtattttcc cgttttttga aagaaaaata aaaaatggaa cggattatgt atatatacat
     1141 aatccgttcc attctttcta ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa
     1201 taaatgactt catgttcgtg ccagggtttg accattagcg aatgatcgaa ccgttttttc
     1261 agttcttcga tgatttgatc atgtgtccag tttcttgcag gaaaatgttg gattttctct
     1321 gcatcgatca aaaagacaaa atcggaatga aaagtctggg taacttcttc cgagagagaa
     1381 ttcaagcgtt cgattggatc cataaaatca cctttcgact taaaattggg tagtatctgg
     1441 gtcttttgct ttgccgacta caccatctaa ctgatcgatt tgtttcattt cttcttctgt
     1501 taattcaaaa tcaaataatt cagtattttc tttgattctg cttggtgtca ctgacttagg
     1561 aagaggaagg aattcgtgtt gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc
     1621 atgtgcttca gcgatttgtt tcatttcagg cacatcgaag attttaccag tgcctaatgg
     1681 actatatgct tcgaggatca tatcatgttt ttttgcatac tcaactacag ttgtctgtaa
     1741 ttcgcctggt gcaaggaaaa cttgattgac catcggcata atttttgctg tttttgctag
     1801 tgtatctaga tggtggggta agaaattgct cacaccgatt gctttgattt tacctgcttc
     1861 atacagttct tcaaatgctc gccatgtatc tgcatttgct tgttcccaat tatcacgaaa
     1921 agcaacaggg tttggccagt ggatcaaaaa cagatctaaa taatctgttt ggagcttctt
     1981 caatgattct tcaaaagaag acatcacaag ttcatagctg tggtttgcat tccataattt
     2041 tgttgtcaag aatatctctt ctctaggaat tccactttcc ttaatcgctt ggccgacgct
     2101 ttcttcattc ttgtatcctt gggcagtatc gatatggcga taacctgcag ccagcgcttc
     2161 tttaacagag gaaacagcga catccccatc aggtgtttgc catgtgccga atcctactac
     2221 cgggatttcg taaccatttg atagacgata agtgcttgtt aatgaattca tactttcagc
     2281 tcctttttta ttcgttcaac tataagtgta gcgcattttt cgaaaaaaag aatccatttt
     2341 gtttctactc ttgaaatttt tgtttaaaag aaaatgattt tgattctggt atttttgatt
     2401 ttttttccgt aaactaagac agaagggaat gagaaaatgg atatctattt aaaaaaagca
     2461 attctgcata ttattgacag agaaactggt tctccagtct tttcacaaaa agagctggat
     2521 ctgacaaaag aatatattcg tgattttctt caaaagaaga tccaaaaaat ctcttctgct
     2581 caaacaaaaa ctggacaact agcagaagaa agcatgatcg ttgaatcact taaaatcgct
     2641 caagaaaatt ttgtggaagc aagtgaagca atcgttcagc ggtggttcga tatctaccaa
     2701 gaaagtgaag aagcacctag tgcagatgta tttgttgtct tgtatgaatt agatacagtg
     2761 atgtacttat ctttattgaa agtcaattat cgtgaggctt acactcactt tgttgaagca
     2821 gacgaagcag ggatcgacaa ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca
     2881 caaaaagcag atgaagcatt cgctgtgaat ttgactgatt ggacctacga attaattgaa
     2941 aaaaaatatg agttttctgg caaaaaagag ctttattttt ctggaagagt gatcgaaagt
     3001 gttcctgcac cttctttaga agaaaatgta aaagtgatcc aaaaagtagc caagcatctt
     3061 ggaaaaaaat ttgagacaga agaatttgat atcatggctg atttgaaaga agctgtctat
     3121 gatacgatag aagaaaaagg tcggttggat catgagatga ttgcggaaaa agtcttcaag
     3181 gaaaacatca cggcaaaaat ggcttttaaa gaagaagtac aagagcaagg atttgtccca
     3241 gaagcacctc ctgtaaaaga agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa
     3301 ttcaagctat caaacgggat cgaactgatc gtaccagtag atgtatatcg caacccagat
     3361 ttgattgaat ttgtgaataa tccggatggt acgatatccg tgatgatcaa aaatgtggat
     3421 gaagtattga atcgtttata aaaatgacta gaatattagg tgttcaagta ccagtgatca
     3481 gttaaatgaa ttgtgtcaag agaaggatcg gaaaagacga atataacaa
//
LOCUS       DAYYKN010000197         3471 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000197 DAYYKN010000000
VERSION     DAYYKN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.200"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHBB7_002761"
     CDS             complement(<1..2232)
                     /locus_tag="ACHBB7_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9210646.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHBB7_002762"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHBB7_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          973 a          769 c          549 g         1180 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt c
//
LOCUS       DAYYKN010000198         3444 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000198 DAYYKN010000000
VERSION     DAYYKN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.201"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..1289
                     /locus_tag="ACHBB7_002763"
     CDS             3..1289
                     /locus_tag="ACHBB7_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM9210647.1"
                     /translation="MVSQKRKYFYLGFTGVFFLFIGLLSNTPTDNWSGLLTILTSPSN
                     LLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLY
                     NSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFGMRFPLLIGILLGNLA
                     GIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNE
                     YHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTTDFIANSGIGATLINM
                     GLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTF
                     HEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGF
                     STGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1255..1605
                     /locus_tag="ACHBB7_002764"
     CDS             1255..1605
                     /locus_tag="ACHBB7_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210648.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1697..2116)
                     /locus_tag="ACHBB7_002765"
     CDS             complement(1697..2116)
                     /locus_tag="ACHBB7_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM9210649.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2117..2461)
                     /locus_tag="ACHBB7_002766"
     CDS             complement(2117..2461)
                     /locus_tag="ACHBB7_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM9210650.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2811..3407
                     /locus_tag="ACHBB7_002767"
     CDS             2811..3407
                     /locus_tag="ACHBB7_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9210651.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          890 a          632 c          629 g         1293 t
ORIGIN      
        1 gaatcgtttc acagaaaaga aagtattttt atcttggttt tacaggtgtt ttttttcttt
       61 ttattggctt gctttctaat actcctactg ataattggag tggattgcta actattctta
      121 cctcgccaag caatcttttg acagattatt ttgctttagg tgttttcgga agcgcattca
      181 taaatgtagg gattttgacc ttactttctg tgcttttagc ttatcggcac aaggtgattt
      241 taaacggtcc cttatttgct tcgattctaa cagttaccgg tttctctttt tttggcaaaa
      301 acctttataa ttctatctca atcattttag gtgtgtatct atacgctgta tttgtcaata
      361 aacctttttc tcagtacatc atgatcggac tgtttggtag cgtattatca ccggtcgtta
      421 gttatatcac ttttggtatg cgttttcctt tactgatagg tattttattg gggaatttag
      481 ctggcatagc aattggtata cttcttccgc ccttagctgc tcaaacttta gttttccatc
      541 gtggattcac actttataat attggattca cctctgggtt gattgcgatg acttttactg
      601 ctgttttgag gttattcagc tattcaatcg tcgaaaatac attggttttc aatgaatatc
      661 actttcctct catttggatt atttttggct ttttttcgct gacagtcgga attggtttct
      721 attataattc ctttcggctt tcggggattc gagaaatttt cgattcatct ggaaaattga
      781 caactgattt cattgctaat tctggtattg gtgctacttt gatcaacatg ggcttagttg
      841 gtctgatgct gtcttcttat gttttgctgg ttggcggtca gttaaatgga cctgtcatcg
      901 gtgctatttt atctgcggtg ggtttcagtg cttttggttg tcatttaaaa aacagttttc
      961 cgatactagt cggaatattt attgcttctt tgtttggaac ttttcatgag atcaccagta
     1021 ctggcatgct tgttgcagct gtgtttggga caggtcttgc gcccataagc ggtttttacg
     1081 gcagttttta cggtgtaata gctggtgtgc tacatattgc tcttgtccat aacgtcagca
     1141 ctttgcacgg aggactaaat ctatataaca gtggtttttc aactggtttc gttgcaggta
     1201 tccttgtacc gatactagat aactttaccg ctgtcagaaa ggaaaaaaag acacttggaa
     1261 aaagaattat caaaaagaat catcgatgag cttcttactt atttatattc ccatgaaata
     1321 actgaaatac gtattggagt agaatttgct gaggaagggc ttttcataga gatacagggt
     1381 acggcagaaa ctcagccaga agatttagat gtacttgctg atttgctgaa caatcctcgg
     1441 gaccctagta tggaaatcta ctatcaagaa ctattaggta atccccgaca tgatcgggaa
     1501 gattatcatc tgttaggtag tttgattgat gacgtggaaa tcatgtatgc ccagccgatt
     1561 atcaatatta agatctttcg acgtgcttct cattcctatt attaacccta ttttctattt
     1621 ataaaagact gaatcaaaaa agcagctcct gaaagttcga ttaaaatcta ctttcaggag
     1681 ctgttcaagc ttgacattat tgattttcca atccttcata cacttcatct gttacaggtt
     1741 ctaaaaactc tgaattacca gcagtgatcg ccaaatgttc aaaccaactg tcttttgtcg
     1801 caccatgcca atgtttaatc cctttgtgcg ttacgacgac atctccagcc attaatcgtc
     1861 ttgctggctt tccttcttcc tgataccagc cttctcctcc ggtcacaagt aagatttgat
     1921 agccgtctgt atggacatgc cagtgattgc gacaacctgg ttcaaaagtt acattaccta
     1981 caccaacaga aacttcggga tcattcacta acatattcag atagctttgc ccggtaaata
     2041 cttgagcgta agcttcgttt ttttcacctg ctggaaaaat cacgccattg tgtatttctt
     2101 cgtgttttgt catcatttac gcctctttct tatcttcgct attttcttct aaatagattt
     2161 cttttgctaa actaaaagct gaccaagctt ttggccattc tacataaaaa gctaaatggg
     2221 taatgatttc tgctatttct tcactagtaa tgccattttg cttgccaatc ttcagatgag
     2281 ctggcaattg ttcaaaattt cctcctgaaa ttaatgcaga tatggtgatg atactacgct
     2341 gatgagcaga taattcttct ctggaccata cttgtccaaa taaaacatca tcatttaatt
     2401 ctgcaaattt tggtgcaaaa tctcctaatt gatctcgtcc tgcggtttgt ttttctgcca
     2461 tttctcttat tctcccttca cgactgttag gtgattcaac tatggttcta aggcaacttt
     2521 attttctatt gtttcttcgc atttcatttt aaatgtagtc tttcagcctg tccaatgctt
     2581 agtttattca gtcgatatgc tttttaagca tagttgctaa cgcttttttt attttaaaaa
     2641 aaacaagtta ttaaataagt ctttagttgg atgttttttt gagatcaatc ttttatactt
     2701 atgatgaact tacaaaattg tttgttttct gtcatatcaa aaaatggcgc agttagttgc
     2761 ttttctagta aagtatagtt aacaaagaac ctctaaggag ttgatgaata atgaatattg
     2821 ctgcctctcg aaaactgata aatttcatat ccattatcgg attaggccta tcaatcgcgc
     2881 tgacgattta ttttattaat ttaggtgttt tcaaagattt gaatgcgtta agaggattag
     2941 taggtgattc catcatttta ggaccgatta tttttgtctt catccaaatc ctgcaagtag
     3001 ttattccaat cattcctgga gggatcagta ctgcagctgg tgtcctgata tttggccctt
     3061 atgctggatt tatctataac tatattggaa tctgcattgg ttcgattatc atttttctgc
     3121 tagggcgtcg ttatggaaag ccattcatac tttccatgat tagcgataag acatataaca
     3181 aatatattgg ctggctggat aatcaaaacc gttttgagaa gctgtttgct ctagctattt
     3241 ttcttccagt tgctcctgac gatgcgttat gtttgatggc tggattgaca aacatgtcag
     3301 tgaaacgata tacactaatc attttgattg ctaaaccact gtcgattttc ttatatagta
     3361 tggccttgat ttatggaggt caatatctaa gtggtcttct tggataatat ttattttgta
     3421 aaattagtca ttgattgaca atgt
//
LOCUS       DAYYKN010000199         3391 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000199 DAYYKN010000000
VERSION     DAYYKN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.202"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(565..840)
                     /locus_tag="ACHBB7_002768"
     CDS             complement(565..840)
                     /locus_tag="ACHBB7_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM9210652.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            947..1711
                     /locus_tag="ACHBB7_002769"
     CDS             947..1711
                     /locus_tag="ACHBB7_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM9210653.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1834..2337
                     /locus_tag="ACHBB7_002770"
     CDS             1834..2337
                     /locus_tag="ACHBB7_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9210654.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            2616..3263
                     /locus_tag="ACHBB7_002771"
     CDS             2616..3263
                     /locus_tag="ACHBB7_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM9210655.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     regulatory      3305..3371
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT         1146 a          595 c          687 g          963 t
ORIGIN      
        1 gaaaaatttt gaaaaaagac aaaaaatcaa gaaagaaata cttagaaaga aaagagtgat
       61 tccaagtgtt tctttcttat ttaaaaaact aaaaactttt ttgttctgtc tggaaaatat
      121 gggttaagca aattcattgc aagttataga tcagcttttt tactacctgt ctttaatact
      181 tgaggcaaag cgcctcaaaa acaaccttat cgacggtgtt caaaagcttg catctacggc
      241 attaagtcca gcctgttgaa aaatatggag agctatttat cacaaccagt ccttaatgct
      301 tgatgcagac cagctttttc acaaccttat tgacggtgtt ttatcagctg cctctcggta
      361 ttaagtcatt acatttcaaa atatgagaag ctattttgaa aatgtttctc cttaatactc
      421 gaggcaaagc gcttcaaaaa caaccttatc gacggtgttc aaaagtcagt ttctcggtat
      481 taagtcaaat ttatgcaaaa atgtggaaag ccattttcac aaaattttcc ttaatactca
      541 aaactaagcg accttttcac aaccttatcc cactacatca aatttctttc gttcctcaat
      601 catcggatct tcttggacat ccacatactg cactcgtcca cttgggctcg gagacttctt
      661 gacttcttcg ataaattttt ggatgatgtc gtcatttccg attgcttcga ttgtgactga
      721 gccgtcgtct tcgtttttta ccgttccggt cacgcctaac tgatcagcaa ccatcttggt
      781 catatagcga aatcccacgc cctggacacg tccttggaca ttcattttaa ctttgcgcat
      841 gttcgtcacc tttctttcct gttttataaa tcaatgatac cgattttttt aggatgaaac
      901 aagctcaagc acctattcca tgatataatc agttgaggtg aggaatgtga aagagatcca
      961 atcagcaaaa aatccaagaa tcaaagaatt aaaaaaacta cataaaaaga aatacagaga
     1021 agaaaaggca gaatatcttt tggaagggtt ccatctgatc gaagaagctg cgtcgtctaa
     1081 tgcagaaata aaagaacttt ttgtgacaca gagaggtatg aaagaatggg gagactggct
     1141 ggctgtaaac aaagagattt ctacttttct agtttcagat gaggtcatga ccgctatgtc
     1201 agatctacct acaccgcaag ggatcttagc agtgatgacg ttaacatctg aagatacgcc
     1261 agatttttca ggaggctggc ttttactaga taatgtacag gatcctggaa atgtgggaac
     1321 gatgatccgt acggcagatg cgtttggtat ggctggtgtt ttattaggta aaggaacggc
     1381 agatatttat agtacaaagg tgctgcgaag catgcaggga agcaattatc atttgcctgt
     1441 gttacaaaaa gatttggcag atgtaatccc tgtaatgaaa ggcaaagggt ttacggtgta
     1501 cggtacagaa ttaaatgaag aagcacatgc attagacaaa gtagaaaaaa cagaggattt
     1561 cgctataatc atgggaaacg aaggtcaggg agtaagtcag gaaatcttgt ctttgactga
     1621 tcagaacgta tatatcccaa tgaaaggaaa tgcggaatca ttgaatgtcg gagtcgcagc
     1681 agggatcgtt ctttatcatt tttctttatg attaaggaac tattaagctt tagctatttt
     1741 atctagagcg actagaataa tccgaacact ggtggtatac ttagatcaat ttcagaagaa
     1801 aatgaaattc aaaaaggtgg cagatcgata attatgacag aaaaatggcg tgaagacgaa
     1861 gaatatatgt cttatgtaca agatctttta gaaacagaag cagtgaaaaa attagcgaat
     1921 tacacacagc atgttcattc cactcgtttg gagcattcca tcagtgtttc ttactatagt
     1981 tatctgctcg caaaaaaatg gggtggcaat gctaaagcaa cagcgcgtgc tggacttttg
     2041 catgatctgt tctactatga ttggcgtacg acgaaatttg acgaggggac acatgcgtat
     2101 atccatccaa gaattgctgt aaagaatgca gaaaaaatca ccgatttgtc ggatcttgag
     2161 cgagatatca ttttgaagca catgtggggt gcaacgatcg caccgcctaa gtataaagaa
     2221 ggatatattg tgacattcgt ggacaaatac tgcgcagtca aagaagcggc tcagcccatg
     2281 tctgcaagta tgcgtaagag atggcaacgt tactttggga aagaatcctc gatttaatag
     2341 gtaaaaccaa taataataga ctatattccg agaaaatctg gctgattttc tcggtttttt
     2401 ctgtttgcag ttgactatct gttcaagata cggtttactt ttatttatcg aatgaatcat
     2461 gtacatagaa gaatgaacat taaagggggt gatgataaga tgcaaaacgt atcttattta
     2521 aaagagtccg ggatcttcag gtaacacata ctttgcagaa gatcactttt ttctacaaag
     2581 tatgcacttt ggttagaaga aaggacttta aaatcatgga acaaacaata aaaccatacc
     2641 aatatttttt tataaaaaga gaagtagatc agttattaaa tgcgtactca tctgtcaatg
     2701 atccaaaaac ggtacaaaca gttcaggcac tagcagctga aaagattcga gacatattag
     2761 aacacgaact gccagaaatc gatacatttt taacgacagt tttagacgta aaactgacaa
     2821 aagcgcaagc agaaagagca ttggaggagc tgaagcaaat cgtccagccg tttatgcagc
     2881 ctagcaaacc acaaatcgag aaattattcc gaaaagtgaa aaaactcaaa caaccagctt
     2941 ggaatgaaat cgacttgcaa gaacatacgt acatcggctg gaatgatcca ggtacgcaga
     3001 aaaaattcat ggtctattac gaagatgaaa aattaaaagg gattagcggt actttatcac
     3061 caacgataaa taagggaatc tgtgcaatct gtcagaaaac atcgaatgtg tcgctttttc
     3121 tttctacaac gaaagcaggt tctgatggca cgtatacgaa aaaggggaat tatatttgtc
     3181 atgatagtga ccaatgtaat caacagttaa cgaaattggc tcctttctat gaatttgctg
     3241 aacgtgtaaa gaaagaacga taattttatt gttttttaca gaaaaatatg ctatctttta
     3301 atggaagtga ataggtgatg gtgttcgcct ttaaatgcta acgaaagata gctaatgacg
     3361 cctactaaag tctggggata gcccctttct t
//
LOCUS       DAYYKN010000200         3343 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000200 DAYYKN010000000
VERSION     DAYYKN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.203"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACHBB7_002772"
     CDS             <1..189
                     /locus_tag="ACHBB7_002772"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210656.1"
                     /translation="DLEKLTKQRDMKNFNNCVVLIIITELTLFTNIYYIHEAQSSLIE
                     DWAIFMAKEGKEMFQNKG"
     gene            202..477
                     /locus_tag="ACHBB7_002773"
                     /pseudo
     CDS             202..477
                     /locus_tag="ACHBB7_002773"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            504..803
                     /locus_tag="ACHBB7_002774"
     CDS             504..803
                     /locus_tag="ACHBB7_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM9210657.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            977..1528
                     /locus_tag="ACHBB7_002775"
     CDS             977..1528
                     /locus_tag="ACHBB7_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM9210658.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1674..1967
                     /locus_tag="ACHBB7_002776"
     CDS             1674..1967
                     /locus_tag="ACHBB7_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM9210659.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1960..2256
                     /locus_tag="ACHBB7_002777"
     CDS             1960..2256
                     /locus_tag="ACHBB7_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210660.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2331..3329
                     /locus_tag="ACHBB7_002778"
     CDS             2331..3329
                     /locus_tag="ACHBB7_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM9210661.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1255 a          458 c          663 g          967 t
ORIGIN      
        1 gatttagaaa aacttactaa acaaagagat atgaaaaact ttaataattg tgtagttttg
       61 ataattataa ctgaattaac tttatttaca aatatatatt atatccatga agcccaatct
      121 tctttaatag aagattgggc tatttttatg gcaaaggagg gaaaagaaat gtttcaaaat
      181 aaaggataac gttatgtcac aaagggagta atggacagtt tgccggtgga attacaagct
      241 ttatgttgga atctaatcga tcaaaacgtt caaaagcaat ttcctttaga ctatcttcaa
      301 atttttgaat tttctacgga aaaaggaaat caaaagttgg ttcatcgtca agaagaaccg
      361 gaagaaagaa aagagtatct gatttcacct aagcttcgtt tgaaatcagt gtctcaaaaa
      421 atatgggcca ttgattcggg tgagtatcaa acgatgttat taccagaaga atactagtat
      481 ctaaaagaaa ggaacgaaag gacttgttag aaacggctat cgaagtactg gaaaagtgtg
      541 cacagctggt cactgcttct gaagagtggg gctatgaatc cgttacgatg gaaaaagagg
      601 aaattgaaat gggtatgctt cctgaagatg ttcatctacc acgtttagtg atgacacatc
      661 tttatattta ttgcgcacca gaagatggaa aagactatgt agtttatttc attacagata
      721 ttaccagcca gcgagaattt gttcgtgggt tactggtaga aggacgattg gtttggagtc
      781 agattggagg aacggatgaa taagatcgat tatttgagaa tgattgcgaa atgtttagaa
      841 gatatcctta ccgcgattca tcaaattctg aagaaaaaag gagaaagaat atgaaatatt
      901 ttcaactagt attgacactt ataacggcaa tggtaattga agtgcttgaa caaaaaatag
      961 cataggagaa aaagagatga ttgaaaaaga attagtgacc acacaaacag aagtttggcg
     1021 agatcctcat catacccatc gctatgtttt caaacgacaa tgggcgatca aaggaaaaga
     1081 agcggaagat aaattagcag tagtcattac tattcgtcca acagatacag aaccatttac
     1141 gaatgatttg agtatgttat taatcgaaaa gaatatccga caattaggat ttactggttt
     1201 tattgctgtg aatctttttt cgtacacaaa agcgaaaagt cctcgggtat ttcttcgagg
     1261 aaatgatgaa caatctttgg aaatattaac gactgttttt acagaaaaga aaatcaatac
     1321 cattattttt gcttgtggtt cgattacagc gacgagtcaa gtggcttatg aacaagcaaa
     1381 gactatttac gatcaattga gtgcgaaaca aaagaagatg accaaagtat tgataaatgc
     1441 agaaggtaag ttatcccacc cactaagtat tcatgttcgt aatgaatggc aattggcaga
     1501 tagtcaatta ttatttcaag aaaaataaca ttttattttc tcatgttact tataagtagt
     1561 tttacatgct ttttgacttt aaaaatacat gtgatgatac atgttatatg aattatcaat
     1621 gtacccagta atatgctatt ttcattatgt aaaaaataac agggggtgtg tagatggaga
     1681 ttgatgaaac ttgtttgagt aactcttata aaagtatgaa tgactttttt gaggaaatgg
     1741 aactggattc agaattgctc tatcaatttt atcttgcttt tcgagggcaa cagatttctt
     1801 ttccaatgaa aatgtacgat cgtgagttag ttagaaaaag aatcgaaaac atgatagaac
     1861 aggaaaaaac tgtagatatc cgtcaattga cagaagttta tggtttctct gcaagatgga
     1921 ttcaaaaagt aattaaacaa aaagaaagga gacgagtaca tggctaagct gattgtatta
     1981 ggtggtttaa tttacggtgg atattggtta tatcaatata tgcttgcgca tccgaatata
     2041 gataaaggaa aagtaattaa gacagttggg gtaatcatag ttggactcat agctctaact
     2101 aagaaaaata atactaatag cccaaaaata atgagaactt atcgctgtag aggatgtgga
     2161 agggaaattt ctactagttt taatccatct gagagtcctc aaggaagaga ccgctgccat
     2221 acgggctatc gacattattg ggaaagaatc gattaaaaga ttaccattta gatcaattaa
     2281 ttgtctgata gaataagtac gtgagggaga aattgaaagg gggaaatata atgaaaaaat
     2341 ggctgttatt actaatagga atgggaattt tattcactgg ttgtacatct gagaatacga
     2401 ggactaaagc gactatagaa actgatacaa caattgagac taaaaaaagt aaaacgcaat
     2461 caactgaaca aactacaaca gaaatgaagc aatctgtgga aacaagtgca agttcaatca
     2521 ctgaatcttc ttctgaagag gttaagaata ctttgtggga tacgaataaa gcttccaaat
     2581 tagaaacttt tgtcacacaa tggggaaaga cattaggtca agaatataaa agctacaatc
     2641 ttcagaataa tgtaagtctg tatggcactc ctttaccaca agccgtgatt aatggcgatt
     2701 ggaaaatggc aatcaatgaa gcaccagtta ccgtacaatg gtcagaagat ggaactggtc
     2761 atgcagattt caatttagta gcagtttatt ctgatgtaga aactggagaa tatttagggc
     2821 agcatgttta ctttttcggt tttcaaaatg gacaacctaa agtttattta acgcaacaaa
     2881 atcaaggtaa tgaaaataat tatctgtact ttaacgaaac tcaaaatcaa cagttaaaac
     2941 aaggtttcag tgatattgtg aatggacaaa ctcctcaaac accagtagta gaagaatcta
     3001 ctcaacaagc aacaactaca acgacacaat ctacaagtag ttcaggaatt tcaagttggg
     3061 aagctgctaa agaatctgtg ctaaataata aagctttatg gttgtatgat tctcctgata
     3121 aaaatgtatc aaatactaat ttaattagca caaatgaata tatgaaagat ctaatgcaag
     3181 atgaaaatgg aagctactat tcaattaata ttgtaacgga tgatgatgga aacacgacca
     3241 tggctggtgg aaaacaattt cgtgtttata cagatggaag aatttctcaa cgtattggaa
     3301 tgactaacaa ttttcaaatg attaaataat tgaaaatatt att
//
LOCUS       DAYYKN010000201         3237 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000201 DAYYKN010000000
VERSION     DAYYKN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.204"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..503
                     /locus_tag="ACHBB7_002779"
     CDS             75..503
                     /locus_tag="ACHBB7_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM9210662.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="ACHBB7_002780"
     CDS             490..732
                     /locus_tag="ACHBB7_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210663.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="ACHBB7_002781"
     CDS             complement(1032..1247)
                     /locus_tag="ACHBB7_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210664.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="ACHBB7_002782"
     CDS             1354..1668
                     /locus_tag="ACHBB7_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210665.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="ACHBB7_002783"
     CDS             1772..2950
                     /locus_tag="ACHBB7_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9210666.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
BASE COUNT         1067 a          487 c          649 g         1034 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagta
//
LOCUS       DAYYKN010000202         3208 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000202 DAYYKN010000000
VERSION     DAYYKN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.205"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(428..1042)
                     /locus_tag="ACHBB7_002784"
     CDS             complement(428..1042)
                     /locus_tag="ACHBB7_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9210667.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1648..2025)
                     /locus_tag="ACHBB7_002785"
     CDS             complement(1648..2025)
                     /locus_tag="ACHBB7_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210668.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2325..3206)
                     /gene="galU"
                     /locus_tag="ACHBB7_002786"
     CDS             complement(2325..3206)
                     /gene="galU"
                     /locus_tag="ACHBB7_002786"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM9210669.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          977 a          659 c          496 g         1076 t
ORIGIN      
        1 tatttctatt agaaaaaggt atcctgttgg acaattttgt tatatcaggt aaacttcatt
       61 gcaataatga taacaaatgt actataatta ctttgtaaag agttttgggg aagactcttt
      121 catcaacgtg tattaagaga caagaaagaa tccatatgga ttctttcttg tctctttttc
      181 cactactttt tatttttctg acactacata ttcttataac tcatttctca taactgtgaa
      241 acaacttata aaaataacaa actttaaaca aaacaagctt aaaatcaaag atgatccatc
      301 ttaaaataac taaaacaaaa aaagtagtcg ttgttaatcc tcattaataa tcgttttctt
      361 tttttcaaac gctccatttt tatgtggaac attatttttt taaggaactt tgtcaacatg
      421 tggataacta ttttccactt gtgtcctgga ctagctgttt tgctgtaaaa tacaaaactt
      481 gctgcagcaa aaagatcgtt gcaagagagg ctgttccgca gatcgctgga tctagatttg
      541 gcaaagtcaa gactgcatca tgtaaattgg cgtggttgtc caaaaccaaa tcagcaaatt
      601 caaataatcg caaaccactg ctatgttgcg atttgatact tcgtgacaaa tcaaatccag
      661 taacaatgat taatggatga ccattttctt tgatccactg agctagttcg atgattgccg
      721 ggtttcttcc ttcattcgaa agaagaagaa aaatgtcttc tgatttgaca gaaagctgat
      781 caataattgc ctgtcctgca ccttctagcg tttcatagac acctccggct ggattttcaa
      841 tcaatatact tggtacaaga tccggggttt gatcccaaaa ttcaaatgct acaccactta
      901 gtgtgcggct agcatatatc tgcaatctac caccttgatt aatttgtttc gcgatccaag
      961 tgccagcttg aagcatcagc ggctcttcta tccttaattt ctcttccaat tggtgataat
     1021 agtcaaaata acataaactc atttatctct cccgcctagc tatacttgac atgaatagaa
     1081 acagctgcct aatctaagga agtacataaa aaaacagtag ttagacagta acacatgtgg
     1141 tctttgctgt ttcaaacaca aaaaaggcat tgaacgaata cgcccaatac cttgacatag
     1201 caaataaaac aacgtgcaga ccatggaaaa tatctacata ggggtagaat tttcacccta
     1261 gattaggtac aataaattag gtcgatgcac tgcattgact atggcaaaat gatcggtcag
     1321 ggtgtatgtg tgtgactggt cattccctga ttcttagcgc caactaaggg tcagggagcc
     1381 tttttttatt cagctgtttc tatctgaatt tattgtaagt aatctcttat ccaagtgcaa
     1441 gcaagaaaat acaaaaaaac attggtatga tttatatata tataaataac ataaaaaaag
     1501 gaatggctta tcccgataca acaagcattc ctttaaaaaa gattctttta tataagtgaa
     1561 aaaagctttt ttccaccaat taaataaatc tctcatctca atagtttatc cattatttta
     1621 tctttttttg cgtataccta ctaaatatta ccgtcttctt ctcgttcttc tactttgttc
     1681 gagagaatct gtactagaaa aagtagttgt atgtctacct tttctgccta tccgagactt
     1741 tcttttcata tatgggatat aaatgattag atacagtaca gcaaaaaata aagcaaatac
     1801 gcaagcttcc caactaccta acgccaattc aaccatcaag gtagcaaccc caacggatgc
     1861 tgcataccag ataaaaatca gaaacgtttc tttttttctg accatgcgaa acatgataaa
     1921 tagactaata agcaatagtg gcaacacaaa tatccaattg cttccttcca tcagccactt
     1981 gagtatatca agcagcgact gtgcaacagc gactaatttt cccatagtga acacacacct
     2041 ttttttataa ttaaaaaatt cgccttttta ttctactatg aatcttctgg cttggctttc
     2101 atttctctta tgtaatcatt ttcttttata caacttaaaa aaaaattaaa ccattagcat
     2161 aatattttct gaatctattc acgaaagtct ttttctatct ttcgttttcc acgactcaac
     2221 tgatctagag tcaaaaaact aaaacgacag ttgcgcaaat gacgcacaag ctgccgcttt
     2281 aataacaaat agcctttcat tctcagcagg ccacttattt ccagttaact ttctgttctt
     2341 agctttttac tcaactggat aatatatgca cttaatgaag tcccaatttc cgaatgttga
     2401 agtccatatt caatatttgt cttcatatat cctaacttat cgcctacgtc gtatcttttt
     2461 cctttaaact ctctcgcata gaccgcctct gtctggttaa gagtctgaag tgcatccgtt
     2521 agctgtatct cgtttcctgc tcctggttct tgagtttcta atatctcaaa tattttagga
     2581 gtcagtagat aacgtccgat aatggctaaa ttacttggtg cttttcccat ttcaggtttt
     2641 tctacaaacc cttttactct gcaacgaccc ttttcgctat ctgttttagg atcaataatt
     2701 ccgtatttag atgtttcatt ttctggtaca cgcatgacgg caacagcgga agcttgtgtt
     2761 tcttcataat ccatcatcaa ttgttgagtc aatgggatat catctttcat caaatcatcc
     2821 cccagcatga cgacaaacgg ttcatttcct ataaatgctt ttgcttgaag cacggcatcc
     2881 cccagtcctc ttggatgaga ttgacggatg aagtgaagat tgacttgagt cgtttcttca
     2941 actaaagcga gtaattctgt tttttccttt tcccgcaaat tgttttccaa ttcgatattt
     3001 gcatcaaagt gatcttcaat gggtcttttt tcttttcccg ttacgatcaa gatgtcttcg
     3061 attcccgaat ccaatgcttc ttccacaata aactggatcg ttggtttatc cacgattgga
     3121 agcatttctt ttgccatggc ttttgttgcc ggcaaaaaac gtgttcctag cccagcggca
     3181 ggaatgactg cttttttgac cttcactt
//
LOCUS       DAYYKN010000203         3199 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000203 DAYYKN010000000
VERSION     DAYYKN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.206"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACHBB7_002787"
     CDS             301..585
                     /locus_tag="ACHBB7_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210670.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACHBB7_002788"
     CDS             588..1004
                     /locus_tag="ACHBB7_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210671.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACHBB7_002789"
     CDS             complement(1141..1860)
                     /locus_tag="ACHBB7_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9210672.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACHBB7_002790"
     CDS             2522..2608
                     /locus_tag="ACHBB7_002790"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9210673.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..>3199)
                     /locus_tag="ACHBB7_002791"
     CDS             complement(2910..>3199)
                     /locus_tag="ACHBB7_002791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210674.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DAYYKN010000204         3135 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000204 DAYYKN010000000
VERSION     DAYYKN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.208"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..899
                     /gene="fabI"
                     /locus_tag="ACHBB7_002792"
     CDS             144..899
                     /gene="fabI"
                     /locus_tag="ACHBB7_002792"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM9210675.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(1009..2061)
                     /locus_tag="ACHBB7_002793"
     CDS             complement(1009..2061)
                     /locus_tag="ACHBB7_002793"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM9210676.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2187..3107
                     /locus_tag="ACHBB7_002794"
     CDS             2187..3107
                     /locus_tag="ACHBB7_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM9210677.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          918 a          626 c          588 g         1003 t
ORIGIN      
        1 caatcttttt ttaatttaag aaaaaatagt ttgacaatca aaacattttg actaataatt
       61 aagaaatcaa gaagttattt tagctattat tctctatttt tttacgacta tcaaattttt
      121 atcagaaaca aaggagagtc attatgtcat ttctagaagg aaaaaaaatc gtcattatgg
      181 gcgtcgctaa caaaaaaagc attgcttggg gttgtgctga agcaatgttg aaacaagggg
      241 cagaaattat ctatacttac caaaatgaac gaatgaaaaa atcattagtc aaattggtcg
      301 gcgaagaagc attcactgta gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa
      361 tgaatgccat cggtgaatat gctggggaga tccacggcct tgtccatgca gttgcttacg
      421 ccaacaaaga agaattgtcc ggaaatgtgt ctgatatttc cagagacggc tatcaattag
      481 ctcaagatat cagcagctac tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg
      541 caccaaacag cgggatcgtt agcatgactt atctaggttc agaacgcgca atcccgaatt
      601 ataacatgat gggtattgcg aaagcttctc ttgaagctgc tatccgttat ttggcagctg
      661 agttctcgcc taaaggaatt cgggtcaatg gaatctcagc tggggcaatc aaaacattag
      721 ctgtcacagg tgttaaagac tatcaaaaac tgatccagtt atctgaatct cgtacgcctg
      781 atcagcaagg tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta atcagcccac
      841 tttccagcgg aatcatcggt gatattatct acgttgacaa aggtgtccat ctttcataaa
      901 cttttgttgc attctttaaa gtaaaacata taaaaatcag gaaaaaactt tttaaagtct
      961 cttcctgatt ttttcttttt acgttttttt caactcatta ctttgaaatt attccacgac
     1021 ttcaatccag ccttctggtg cttctatgtc tccaaattga atacctgtca attcatcata
     1081 caaccgtttt gttactggtc ctacttccgt ttcactataa aatacatgaa gattgttatt
     1141 gtgataaatc ccaccgattg gagaaataac agccgctgta ccacatgcac cagcttctgc
     1201 aaattgatct agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc ctagtcgttc
     1261 ttcagctaac tgcaacaagg aatatttcgt gatacttggt aagatagagg gcgattgcgg
     1321 agtgatgaat tttccgtctt ttgtaattcc aaagaaattt gctgcaccta cttcttcgat
     1381 tttcgtatgt gtggctggat caagataaac acaatctgag taatccagtt cttttgcttc
     1441 tgttcctgga agtaaactcg ctgcgtaatt tccgccgact tttgctgcac cagtcccctt
     1501 aggagcagca cgatcgtaat cagatacaat aaaattcgtt ggttttagtc cacctttgaa
     1561 atacgctcct actggcgtac agaagacgac aaataaatat tctggagctg gatggacacc
     1621 aatgttttca ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc ctgttccata
     1681 gggcggcaca aaatcttcat ttgcttttac cacttgcttg acagcatcaa cgaacatctt
     1741 ttcagggacc attggcatca gcaatcgtct agcactttga ttcatccgtc gactattttg
     1801 ttcaggtcga aagagattga ccttgccgtc ttttcgacga taagctttta gaccttcaaa
     1861 acattgctga ccataatgaa gcgctggaga gccttcatga atcgtcaggt aattatcttc
     1921 tgtcaattct ccttcttccc attgtccgtc tttccatttg gcaataaaac gccaaggagt
     1981 tttgatataa gaaaaaccta gattgttcca atcgatttgc atatacttac caccttcgtt
     2041 attttctcca cattttatca ttatccatat tatttgtcat tttattttca gcttttttcc
     2101 ttcattatag ttatttttgc tatttccttt tttgaaaact tcttggtaca atacagaaag
     2161 aatcaaattt tagaaaagag gattttatgt tttcattatt agcaactgcc ggtacgacag
     2221 actcttctgg cgagacattg acagatcaag ctgtcagcaa agtaaatgcg tttcagcggt
     2281 tttgggacgg gattgattgg gacgccattt tggctgcctt tatccaaaaa agtttatatc
     2341 tgattttttt gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat ctgattgata
     2401 agtcttttaa tcagtattct aaaaaagcca ttctaaacga gagccgctta aagactttac
     2461 atagtctttt aaatactatt tttcattata cccttggttt cttttttatc tatgcaattt
     2521 tatctgctat cggtgtgccg atcggatcac ttttggctgg tgccggaatt gcaggtgtgg
     2581 caatcggtct tggtgcccaa gggttcatga gtgatgttat taccggattt tttattatca
     2641 tggaacaaca gatggatgtt ggggactaca tcaagctagc aaacttaacg attgaaggaa
     2701 ccgtcgtatc tgttggtatc cgtacccttc agctgaaagc aacggatggt actgtccatt
     2761 tcatcccgaa tcgaaacatc accacgatca gcaatctctc tcgtgcaaat atgcaagtac
     2821 ttttagatat tcggattgtt cctgaagaag gatatgacaa aatctatgag atcattgatc
     2881 gagtaaatcg tacattagct gaaaaatacc aagaagagct ccaaactgaa ccttctatct
     2941 ttggattagt ggatcttgga aacagcaatt ttgcgattcg tacggtctgc tacgcattga
     3001 acggaaaaca atatgcctta aaagaagagt tcttgagctc ctatgtcaaa gaattgacag
     3061 cttctggttt cacgattccg aacagtccaa ttgctgtcgg aaaataattt ggacaaacga
     3121 tgcaacgaat ctcac
//
LOCUS       DAYYKN010000205         3066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000205 DAYYKN010000000
VERSION     DAYYKN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.209"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(264..1574)
                     /locus_tag="ACHBB7_002795"
     CDS             complement(264..1574)
                     /locus_tag="ACHBB7_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM9210678.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1586..3004)
                     /locus_tag="ACHBB7_002796"
     CDS             complement(1586..3004)
                     /locus_tag="ACHBB7_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9210679.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          954 a          631 c          475 g         1006 t
ORIGIN      
        1 aaatctttca gcaactcatt tatcataact tatcgtttgt tatttgtcaa gaactttttt
       61 gaagtttttt tcgatagaat caagcttcgc aagtggttct ttttaacgac ttagttatct
      121 tatcacgtct aaaaacatgt gtcaactctt ttttggaatt ttcataaaat gtttcaatga
      181 agcgcgacaa cgttgtttaa tatatcatta aaaaaaataa aggtcaagct aaaatattat
      241 ttaagcttga ccttattata atttcaacga ttgtcaaaag tattgtttgc agctgtgttg
      301 ccagtttcta tagataaatc acagcttttg aaacataagt tttgtactgc ttcgattcgc
      361 ccgaattctt ttgcagttat ttggcaatga gaaaagcaga catcttctat aggcttttca
      421 ggaaatgcta ctaaatcaaa agctcgggaa ggaagtgagt aacctggaga aaggcatgcc
      481 tgtacgtttt tgatgtgtat gtctttgact ttcgtcattt gctttgattc aggtatttct
      541 tcagccactg cttgccaact ttctggcatt tgagcaaggt cattgctatt ttttctattg
      601 tattcattat gccagtccat tatccaagaa aaaggaaatt gtacatcaat catattcaga
      661 ttttctaccc tgatattttc gatcactccc cctctttctt ttgaagattt cattcgaata
      721 ccacagtctg actgaaaaaa atcgatatcg tgaatatata cgtcactaac tccagcacta
      781 acttcgctac caatagttac accgtatcct gaacggattt tacatctagc aacctcgatt
      841 cttgatgatt ttcgattgac tcttgcacca ttgccatctc tgccagactt aatagcaata
      901 caatcatcac cacaggccaa atcacattca tagacacgca catttgttga agaatcaata
      961 tcaatcccat ctgtacttgg tccatcattg ttgcggatga ttactttttc tactaataca
     1021 tcgttagaat aagtgatttg caagttccaa aaacctgatt tctgaaaaat caaatcacga
     1081 acaacaacat gttctgcatg atacagtaaa caagcgcgtg gacgtttaat agcataatcc
     1141 gcaatccaac gaagtccctt ttgatcgtat tcagctcttg tcccgctttt ttgatctttt
     1201 ccccaataca gctcccacca atggtctcct tgaccgtcga ttattccttt tccttctata
     1261 agaatatctt tcgccgagag aacattcaaa atcgctgctg gccatttcat ttctacgccg
     1321 gctactcgtg tatcgatttc tggaaatgct tcgatatctg ttctcccctt gaggactgca
     1381 ccttcttcaa agataagatg actcttatct tttaaaaaaa gggctccgac taaataaatg
     1441 ccttttggaa taaatacagg taagtgttct ttcgcacctt gatcgattgc tttttgcagt
     1501 attattgtat catcttgttg agtgtcgttt gttttaaagt tcaaagcatt gataatccct
     1561 ttttttgtcc gcaatacgtt tctccctatc tttcattgat ttttttcaat tcttcaccaa
     1621 taaatacagt ttttgaacga ttcagtgggt aacctactag caaaatgatt gcggatacaa
     1681 ataagcagaa tgctggtaac aagtttgcca tcgtataaat cctttcttgt actaaaactg
     1741 attggatggc tccacctgtt gtagacgatt ggtaaccaat gagtgccagc ataaatccgc
     1801 ctaccgctcc tgcacatgct tgtcctactt ttctcgcaaa agaatttact ccatatactg
     1861 ttccatcttc tcttaagcct gtcttatatt gatgataatc aatcacgtca gtaatgaatg
     1921 cccagaccat caaattgaat aaaccagatc ctaaagtagc gataaataaa agaatcagat
     1981 atacccatgc atttgttata tgaaggaaat acatcagcaa gtacatacat gaagaagcaa
     2041 gtaacgcaaa tacgcttgct tcctttttac caaataattt tgtcagcttt gtagcaaatg
     2101 gtgctaaaac tactactgtt ccgtaagtaa ataaaagagc aacagacatt gcttgtttat
     2161 tatggaagta atcattgaat aaataagtca tattcgtccc agctaaaatc tgattgatca
     2221 caatgaaaat gtcaacaatg actaaaataa tcagtgctcg attagctaac aaacctttta
     2281 ccaactgaga agcagatact ttttcgtttt tgacaactct tactctttca gtagtcaatt
     2341 tacaagttaa catatatgcg acaaatgcta ttgcagcaca tccgattgaa atccaaaaga
     2401 aatgattccc tgaaacgact tgttgtccac tcttgttatg ggtatacatt aaaatcggaa
     2461 tgaagaatcc tgtagttgat ccgccaatcg cagaaccaat gcttcggaac gtggacaacg
     2521 aggtgcggtc attaggattt gagctaattg ctgctgccat tgctccatac gggatattga
     2581 ttgttgagag aaaaatccca taaacaatgt atgaaacaaa aatataagca atcttcaccg
     2641 tcattggaag atgttggacg aatggcaaaa acaaaatgac agtcactaag caaaatggat
     2701 acttcatttt tctgatccaa ggaataaatc ggccagtttc tgttaactct gagacatcac
     2761 ataatcttcc aaccatcaca tcagcaaacg catccaaaat tctcgaaatc aagaataacg
     2821 tacctacgat tgcccctgaa acgccaagaa cattcgtata ataaatcatc aagaaactat
     2881 tgactaaacc taaaataaaa cagttaccta gatcaccgaa catgtagcct atcttatcgg
     2941 aaatcccaaa ttcctttttt tcttttttta ttgtatttgc tgtaacagca ttttgtgaaa
     3001 gcatttgaat tcctcctacc tcttctagta aacgttttca aaattataac tctttttttc
     3061 ttgttt
//
LOCUS       DAYYKN010000206         3041 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000206 DAYYKN010000000
VERSION     DAYYKN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.210"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            718..1560
                     /locus_tag="ACHBB7_002797"
                     /pseudo
     CDS             718..1560
                     /locus_tag="ACHBB7_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
     gene            1682..2977
                     /locus_tag="ACHBB7_002798"
     CDS             1682..2977
                     /locus_tag="ACHBB7_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9210680.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          944 a          558 c          587 g          952 t
ORIGIN      
        1 aactagatca tcaatcgtaa gcatcgcaaa ttgatttcta ccattcgtat tgtttttaga
       61 catcatagct atcactcctt gcattcgttt tcataaatct attataaaag aaaagactgt
      121 agacatcatc cataaaatgg actttgtcta cagtctggat aacgtgattt tttgctgtaa
      181 tgacaaggtt ttagctgttt cagataccat gtatccaaaa atagtttatt gagaaaatag
      241 gagagtattt gatactaact agtaggaaaa aatgagataa aaagtaaatg gaatcattta
      301 aggtaatctg aaataaaaaa tatacagccc tgtggtactt tacctacaag gctgtatata
      361 ttttttactc tcagcgtttt tgactccgtg tctgactccg ttttattgat tttgaatgtt
      421 attcaatgac catttaacag gtcaaatttg atgtttttga ttccgtatga cgccgtttga
      481 tatctagtga tatagtgatt tgagtctaca tcgaataagg gaataaacca ataaaaaatg
      541 atttagaata ttaaaataac aaaaaagagg actacctgta ttagtcctct tttttgacca
      601 gattttgacc agtctatttt acatcctagt atatttatat gagctaattt tatgattaaa
      661 tggtaagttt ttccttgtgt gttttagttt aaactagtgt gtaattcaaa aatctatatg
      721 gaaggaacag tattaagaat agaacagggt tctctacatg atggtgctgg tttacggacc
      781 gttgtttatt taaagggctg cccattacgc tgtgcctggt gttcgattcc cgaatcacaa
      841 agcaagcaga ttgaaaaagg atttggccag accatgaccg ctgaagaggt aatggatgag
      901 attgagaagg atgctgtttt ttactttcac tcggatggcg gagtgaccat tagcggaggc
      961 gaggcactgg ttcaggcgga ttttgctaaa gaaattttgc aaaaatcgaa atacattgga
     1021 atcaataccg tactggaaac cagcttttgt ggtgcttata atgaaatcca aaaggtagct
     1081 ccctatgtgg atacattgtt tgtggatgta aaaatgttta ccagcaagct ccacaaacaa
     1141 tggaccggct tagataacca acaaattttg aagaatattc gtcgcttttt aattgagtat
     1201 cctaactgcg aagtgcgaat tcgagttcct gtggttccag gtatcaatat gaacctgacc
     1261 gagctgctga cgattgcttg ttttgtggca gatcttgatc ggttcgtacc gttagaatta
     1321 ctaccgtacc actgctatgg tatgcacggt taccaagcat tgggactgga gtatccgttg
     1381 gcagacacgc cagcgccttc acctaaagaa atgtttgcat tggcagatca actggctcgc
     1441 acggttcctc atttgctggt cacagcttta accaaaacat ttatttattt attgaatcca
     1501 agctttatct tatggataaa tgaaagacac tcactagctg cagccagctc aagaatagag
     1561 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
     1621 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
     1681 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
     1741 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
     1801 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
     1861 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
     1921 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
     1981 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
     2041 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
     2101 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
     2161 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
     2221 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
     2281 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
     2341 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
     2401 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
     2461 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
     2521 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     2581 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     2641 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     2701 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     2761 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     2821 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     2881 caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aactacgaat
     2941 tatgattcgt catggcaaaa catttttaac caaataaaca gaacagaaag aggttttctt
     3001 ctttctgttc tgtccaaatt attctaagat ttctctttac c
//
LOCUS       DAYYKN010000207         3040 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000207 DAYYKN010000000
VERSION     DAYYKN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.211"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="ACHBB7_002799"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="ACHBB7_002799"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM9210681.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="ACHBB7_002800"
     CDS             complement(1036..1956)
                     /locus_tag="ACHBB7_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9210682.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /locus_tag="ACHBB7_002801"
     CDS             2069..2821
                     /locus_tag="ACHBB7_002801"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM9210683.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          856 a          612 c          526 g         1046 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat
//
LOCUS       DAYYKN010000208         2998 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000208 DAYYKN010000000
VERSION     DAYYKN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.212"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..534
                     /locus_tag="ACHBB7_002802"
     CDS             <1..534
                     /locus_tag="ACHBB7_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFM9210684.1"
                     /translation="FIKPLNGSSSVNTFIVNNEKELLFFSEYIDNPLIQEYILGEEYT
                     IDVCLNFDSTPVSIVPRKRIATRGGEILKGQIIKDRELIETTKKMLEKLNAIGHITIQ
                     CIKNEKGIFYLEINARYGGGAPISIKSGANSPEYIIKMIDSKKNLRYRENYLDKMIAL
                     RYDQAIYLDEKGNVYND"
     gene            527..1222
                     /locus_tag="ACHBB7_002803"
     CDS             527..1222
                     /locus_tag="ACHBB7_002803"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9210685.1"
                     /translation="MIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIFPATFDEI
                     YRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLENADTLLTE
                     LRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPISYLLLLE
                     KFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFYQADKKIR
                     SLWEIKNFLGVIS"
     gene            1219..2337
                     /locus_tag="ACHBB7_002804"
     CDS             1219..2337
                     /locus_tag="ACHBB7_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFM9210686.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            2327..2947
                     /locus_tag="ACHBB7_002805"
     CDS             2327..2947
                     /locus_tag="ACHBB7_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9210687.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
BASE COUNT         1141 a          370 c          576 g          911 t
ORIGIN      
        1 tttataaaac ctctgaatgg tagttcatct gttaacacat ttattgtaaa taatgaaaaa
       61 gaattgttgt tttttagtga atatatagat aatcctctta ttcaagagta tattttaggt
      121 gaagaatata ctatagatgt ttgcttaaac tttgattcga caccagtaag tattgttcct
      181 agaaaaagaa tagcaactag aggtggggaa atactgaagg gtcagattat taaagataga
      241 gaactaattg agactacgaa aaagatgttg gaaaaattaa atgcaatcgg acatattact
      301 attcaatgta taaaaaacga aaagggaata ttttatttag aaataaatgc aagatacggt
      361 ggaggagccc ccattagcat taaatcagga gctaattccc ctgaatatat tataaagatg
      421 atagattcaa aaaaaaattt gagatacaga gaaaattatt tagataaaat gatagcactg
      481 agatatgatc aagcgattta tttagatgaa aaaggaaatg tatataatga ttaaagcggt
      541 gatctttgat ttagatgaca ctcttgtgtc tgaatatgat tatgtaaaaa gtggatacaa
      601 atgtgtagct agaagattag cagtgcaaaa aattttcccg gctacttttg acgaaatata
      661 tagagtcctt ttagatcttt ttgctgaaga tagtaaaaat gtatttaatc gtctatatga
      721 ctattttgaa attccttata gtaaaaaaga aattaaagaa ctggtaaatg tatatcgaga
      781 acacaaacca gacataaatt ttttagaaaa tgcagatact ttattaacgg aattaagaag
      841 tagaggatac aagctgggga ttattacaga tgggtatgct ataacccaac gaaacaaatt
      901 ggatgctttg aaagtagact atttggtaga ttatatttgt attactgacg aattaggccg
      961 tgaattgtgg aaaccacacc ctatctcata tcttttattg ttagaaaagt ttaatttaaa
     1021 ggcacaagag tgtatatata ttggcgataa tgagttgaaa gattttataa ctgccaaatc
     1081 aataggaatg caaacaatta aaatagaaag agtaaatggt atttacagag aaagcagttt
     1141 gaagttcaaa agattttacc aagctgataa aaaaatacgt tcgttatggg agataaaaaa
     1201 ttttttggga gtgatttcat gaaaagtaaa agaattctat tagcttctcc acatatgagt
     1261 gaagaaggat atgaaaaaga gtatgtaaaa gaagcatttg atacaaattg ggttgctcct
     1321 ttaggtgcga atgtgaatgg atttgagaaa gaattagcag aatacgtggg agctagtcat
     1381 gctgccgcat tatcttctgg aacagcagct atacatttag ctttgaaagc ggctggggtc
     1441 ggtaaagatg acatagtgat ttgtcagtcc ttgacgttct cagctactgc taatccaatt
     1501 ctctatcaag gagctatacc tgtatttgtt gatagtgatt acgaaacttg gaatatgaaa
     1561 cctgaacatt tgcgaacagc actagaaaaa tatcctaaca caaaggctgt attagtagta
     1621 catttgtacg gtttatcagc taatttagac gaaattacta aaatatgcaa agaatacgat
     1681 gttactctaa ttgaagatgc tgcagaatca ttaggaacaa catataaagg aaaatatact
     1741 ggtacatttg gcgactatgg aatttattct tttaatggaa ataagataat cacaacttca
     1801 ggtggcggaa tgcttgtatc caatgacaaa gaaaaggtgg aaaaagtgag attttgggct
     1861 acgcaatctc gtgagaaggc acgccattat caacacaatg aaattggcta taattatcga
     1921 atgagcaata tcgttgctgg tatcggtcga ggacaattga aagtattaaa tgaaagaatt
     1981 gagaaaaaac gatatattca tagtttttat aaaaaagaat tgtctaaatt gtcaaatata
     2041 caattcatgc cagaaaataa ttggaatgaa ccgaattatt ggttaagtgt cataacaata
     2101 aagggaataa atgcgaatca tcttattgat gagttagaca aaaataatat agaagcccgc
     2161 ccagtatgga aacctatgca tttacaacct gtttttaaaa attatgatta tattggcgat
     2221 gatgtagcag aaactttatt tgacttagga gtatgtttac catcagatac aaagatgaca
     2281 gatgaagatt taaaatatgt agtatctacg attaaaagag tgatcaatga tgcgtaaaaa
     2341 aactttctat cagctttttg ggaaaagaat tttagatatc ctattgtcag gaattgctct
     2401 catcgtttta tcgccgataa tcttaattgt aggtttttta gtaagaatta aacttggttc
     2461 tccaattatc ttcaaacaag aacgaccagg aaaaagtgaa aaaatttttt ccatgtataa
     2521 atttcgtaca atgactgacg aaagagacca caatggagaa tatttacctg atgaaattcg
     2581 actaactaaa tttgggaaaa tgcttagagc aacaagtctg gatgagctac ccgaactttg
     2641 gaatattttg aaaggtgata tgtcgatcgt aggacctcgt ccattattag tggagtattt
     2701 gccactatat agtgagaagc aacggaaaag acacaacgta agaccaggat taactggact
     2761 ggctcaggtc aatggaagaa atgctattag ttgggaagaa aagtttgatt tagatgttta
     2821 ttatgttgat aaaattagtt tctttaatga tcttattatt ataattcaga catgtaaaaa
     2881 agtaatcaag aaagagaata ttaatacaat caataacaat atcccagaaa aatttaaagg
     2941 gagttaaata cagcagctta atgcaagttt tgtgacttgc attatattta ggtataaa
//
LOCUS       DAYYKN010000209         2979 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000209 DAYYKN010000000
VERSION     DAYYKN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.213"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..152
                     /locus_tag="ACHBB7_002806"
     CDS             <1..152
                     /locus_tag="ACHBB7_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM9210688.1"
                     /translation="IIFVSIFSLILHILINQMLIRYVLTSLLIISLVIYFLKIRNRII
                     DDYNS"
     gene            165..1172
                     /locus_tag="ACHBB7_002807"
     CDS             165..1172
                     /locus_tag="ACHBB7_002807"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM9210689.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1184..2188
                     /gene="neuB"
                     /locus_tag="ACHBB7_002808"
     CDS             1184..2188
                     /gene="neuB"
                     /locus_tag="ACHBB7_002808"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM9210690.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2185..>2979
                     /gene="neuC"
                     /locus_tag="ACHBB7_002809"
     CDS             2185..>2979
                     /gene="neuC"
                     /locus_tag="ACHBB7_002809"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM9210691.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYI"
BASE COUNT         1101 a          376 c          565 g          937 t
ORIGIN      
        1 taataatttt tgtatcaatt ttttctttga tacttcatat tttaatcaat caaatgttga
       61 ttcgttacgt tttgacatca ttattgataa ttagtttagt tatatatttt ttaaagatta
      121 ggaatcgaat tatagatgac tataattcat aggaggagat ataagtgttt aaaggaaaaa
      181 caatattagt aactggcgga actggttcca ttggaagtga aattgttcgt cagcttttaa
      241 aatatgaacc aaaagctatc agaatttata gtagaggtga agaaaaacaa ttttatatgc
      301 aacaagagct ctcaaatcac actaatttga gatttttaat tggtgatatc agagactctc
      361 aaaggattga ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc gcaatgaaac
      421 acgttccagc aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg atgggaactc
      481 aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt atcagtacag
      541 ataaagtagt gagtcctact aacaccatgg gtgcgacaaa gttattgagt gaaaaattaa
      601 taatagctgc caatcagtat aaaggaaaaa cagaaacaat attttcttgt gtaaggtttg
      661 gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt tagtaaacaa ataaaaaaca
      721 ataaagatat tactttgaca gatgttgata tgacacgatt tatgatgtca ataccggatg
      781 ctgcaagatt agttctgtct gctgctatga attcaaaatt tggagaaaca tatgtcttaa
      841 aaatgccggc aatcaatatt ttaactttag ctcaatcatt aataaaaata tataatgata
      901 cacatgatga aaaatataga ggtgaaataa aagttgtagg tatcaggcca ggagaaaaat
      961 tatatgaaga gctaatgact acagacgaaa ttgaacgctc atatgaaaat gataaaatgt
     1021 atatcattcc accacactat catgctactg acgaactgta caaagattat gaaaaaatta
     1081 aaatcactgc atactcttca gatgcaacag aattaatgag tggacaggaa tttattagaa
     1141 tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt atgatgaaat cagaagtata
     1201 tgtaattgca gaaattggtg taaaccataa tggaagtcta gatttagctt tagaaagtat
     1261 tgataaagct aaagaatgtg gagctaacgc ggttaaattt caaacattta aaacgaataa
     1321 gctagtttcc aaaaaggctc caatggcaaa atatcagaag gataatctta aagaggacag
     1381 ttctcaattt gaaatgctaa aaaaattaga attatctcaa tctgatttta ttagaataaa
     1441 agattattgt atacaaaata aaattgattt tttgtctact ccttttgatg aagaaagtgc
     1501 agactttcta aaacaaattg gtgtacaagg atttaaaatt ggctcaggag atttaacaaa
     1561 tttacctttt cttagaaaaa tagatatgta tgggttgcca gttttattat ctacaggtat
     1621 ggctgatatt catgaggtca gtgaggctgt agattgtttc atagatagtc cagttactat
     1681 tttacattgt acttcaaatt atccggctag ttctgaagat attaatttat tggcgattaa
     1741 cacattaaaa gaaatgttcg ggatacccgt tgggtactcc gatcattcat tgggatatga
     1801 tgttggtatt tgtgctgttg cgatgggtgc taaggtaatt gagaaacatt ttaccttgga
     1861 taaggaacta cctggtccag atcataaagc ttctttagat cctcaggaat ttgcagattt
     1921 tgtaaagcat attagaaaca ctgaaatatt tttaggtgat ggtgttaagc gagcaatgcc
     1981 ttctgaactt agtacaaaag aagtatcaag aaaaagccta gtggtcactg agaatttaaa
     2041 tgtgggagat attcttacag agaaatctct tacaataaaa cgtcctggaa atggaatagc
     2101 tcctaagttt ttatatcaat atattggcaa aaaagttaaa agaccaataa gtgcagatag
     2161 tgttctgctt gaggaagatg ttgaatgaaa aaaatactta tagtaagtgg aacacgagct
     2221 gattatggaa tatataaacc tgttgctgag aatttactac aagataattg ggatgtatca
     2281 tttgttgttt ctggaatgca tttatcccat aaatatggat ttactaaaga attgatctta
     2341 gctgacggat ttagaattag tggagaagtt caaagtttgt ttttagagaa caataaggga
     2401 aacatggctc ggtctgtggc attagagata aatggtttca caaatatatt tgaacaaact
     2461 agccctgatt ttgtattatt actaggggat agaggagaaa tgttggcagc tgcgatagct
     2521 tgtttgtatc ttggaatcaa aacagcacat attcacggag gcgaagtgtc tggtactgta
     2581 gatgaatcaa taagacatgc cataacgaaa atagcatctg ttcattttgc tgctacggaa
     2641 aaaagcaaag agcgtttaat aaaaatgggg gaagattctt ggagagtttt tcccgtcgga
     2701 gctcctagaa tagatactat tttaaataat agattaccta gttttagaga tataaaagaa
     2761 aaatatcatc taaaatttga aaagaaaaaa tacgttttgc aagtatttca tccagttgta
     2821 accgaactag aaaatataga aaaacaaatt ttagctttga tcaattcaat ggaacaacaa
     2881 tctcaaccaa ttatttgcat tttacctaat agtgatgctg gtagcgaaat tatcagaaaa
     2941 atgtaccaga aatatttaaa aacggatatt atttatatt
//
LOCUS       DAYYKN010000210         2938 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000210 DAYYKN010000000
VERSION     DAYYKN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.214"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..1657)
                     /locus_tag="ACHBB7_002810"
     CDS             complement(365..1657)
                     /locus_tag="ACHBB7_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9210692.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="ACHBB7_002811"
     CDS             complement(1931..2191)
                     /locus_tag="ACHBB7_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210693.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
     gene            2442..>2938
                     /locus_tag="ACHBB7_002812"
     CDS             2442..>2938
                     /locus_tag="ACHBB7_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM9210694.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT         1007 a          547 c          452 g          932 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
     2341 gtaaaatatt ttgtgtaaat gaaaaaatcc atacaaaaaa ggaagtccct tctgtagaat
     2401 aaagttaacg acaaccaatt cacagaaaag aggacttccc tatgaatgat tttactacag
     2461 aaattgtgca aactctagtc actaaaggcg atttaaatga attattccgt tcgcacttag
     2521 aaaaagcgat aaacacactc ctacggactg aattaacggc ttttttagat tacgaaaaat
     2581 atgatcgcac tggttttaat tcaggtaatt cgagaaacgg ttcttacttt cgatcaatca
     2641 aaaccgaata tggtgaatta acattggaaa tacctagaga tcgtaatggt gagtttaaac
     2701 aacaaacttt accagcctac aaaagaacaa acgatacatt ggaaaccact attatccatt
     2761 tattcgaaaa aggtgttacg atgtctgaaa ttgctgattt gatcgaaaaa atgtacggtc
     2821 atcactatac tccacaaacc atgtccaaca tgactaaagt tctgactgaa gaagtaaatg
     2881 cctttaaatc cagagcctta aatgataagt atgtcgctat ttttatggac gctactta
//
LOCUS       DAYYKN010000211         2881 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000211 DAYYKN010000000
VERSION     DAYYKN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.216"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..665
                     /locus_tag="ACHBB7_002813"
     CDS             18..665
                     /locus_tag="ACHBB7_002813"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM9210695.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            785..2161
                     /locus_tag="ACHBB7_002814"
     CDS             785..2161
                     /locus_tag="ACHBB7_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9210696.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            2171..>2881
                     /locus_tag="ACHBB7_002815"
     CDS             2171..>2881
                     /locus_tag="ACHBB7_002815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9210697.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDV"
BASE COUNT          993 a          342 c          577 g          969 t
ORIGIN      
        1 aaaggaagga gatagatatg aaaaaagtag aaattttaag taagttagaa gcggctggag
       61 tcattgcggt tgtacgtggg aaaaataagg atgaggccat taaagcaata aattcaatta
      121 ttgcgggtgg aattaaaggg attgaactaa cttttacagt tcctaatgct gaagaaataa
      181 tcaaagaaat tgctaaaaaa tatgctgatc aaaaagatgt aataatcgga gccggtacgg
      241 ttttagatgc gtttactgct cgtatcgcaa ttatggctgg ggcagaatat atcgttagtc
      301 catcttttga tcgacaaaca gctgagattt gtaatttata tcaaattcct tatttaccag
      361 gttgtatgac aattactgag ataaaggagg cacttactgc tggcgtagat atcattaaac
      421 tattcccagg ttctgctttt ggtccatcca ttgtaaaagc ctatcaagct ccattaccac
      481 acgtaaatat aatgcctact ggcggagtaa gtatagagaa cttagaagat tggtttgaag
      541 taggagttgt tgcagttggt gtaggtggga atttattggc ttctgttgaa aaaggtgatt
      601 ttgaagaagt aagtcgagta gctagtaaat atatagataa gttttatgag ataaagaatt
      661 attaggagaa aaaacaaata atactctgaa atgtaaaaaa taaatactga ctctttacgc
      721 ttcagagtat agtacgtact ttcatcagaa tgaaaacgct taacaacaga agggagagaa
      781 ttgtatggaa agaaaatttg gtataagaga caaaatagga tatatgtttg gagattttgg
      841 aaacgatttt actttcattt ttgctagttc ttttttaatg atcttttata caaaagtatt
      901 ggggataagc ggagctgccg ttggaacatt atttttagta gcaagattta tcgatgcagt
      961 tactgatgta actatggggc gtattgttga tagatcgaaa gcaactaagc agggtaagtt
     1021 taaaccgtgg attttaagaa tgagtggtcc agtcgcttta gcaagttttt taatgtatca
     1081 aacttctatg atttcagctt ctatgagcgt taaggtagta tacatgtatg ttacgtatat
     1141 attatggggg agtatttttt acacatctat aaatattcct tatggatcaa tggcatcagc
     1201 tattacttct attacagaag aaagaacaat gctatcgaca ttccgaaatg ttggagctac
     1261 tttagcaagt ttggtcattg gtgtattaac accattattt atttatacaa gagatgattc
     1321 aggcgctcaa atagttcgtg gtggatcaac ttttacaatt gttgcgggaa tattttcgat
     1381 atgtgcactt atttgttatt taatatgtta taaactaact actgaacgag taagaattga
     1441 ccaatctcaa tacaaagttt cattgttaaa atctttaaaa gggttagtta aaaatagggc
     1501 attactttct ataattggtg cggctatttt cttactcctt gctcaattat taattatgag
     1561 tatgaataat tatgtatttc ctgattacta taatgatgca cagggaatag ttttaatgaa
     1621 ttttatcaat cctattttgg ttttaatagt tgtttcgcct ttaactttgt ttttaagtaa
     1681 aaaatatgga aaaaaagaac tggcatcagt aggaatgttc ttttcagcaa tagtatattt
     1741 gatcttattt ttgataaaag ctagtaatat gtatgtattc ttaattctta gttcggttgg
     1801 ctatatgggt ttgggtgtgt ttaatactgt tatttgggca aatattactg atgttattga
     1861 tgatcaagaa gtaaagtctg atcaaagaga agatgggacc gtatatgcgg tatattcttt
     1921 tgctagaaaa ataggtcaag ctctagctgg aggcgtcgga ggttggactc tttctattat
     1981 aggctatgac tctttagcaa atgtacaaac gggagatgtt ttagaaaagt tatataacgc
     2041 ttcaacatta attccatcaa tttgttttct gattgtagga ttaattttat ttttccttta
     2101 tccattatct aaagataaag tcttagaaaa ttgcgagctt ttaaaacaaa aacataaata
     2161 ggagagaaat atgaataatt ggatattttg tattgattca gatggctgtg ttatggatac
     2221 gatgacttat aaacacaatc tctttttcgg accaattgct gcagagattt ttgatgttca
     2281 tcatagagaa aaattcctac aggattggaa taatattaat ttatattcag agactcgtgg
     2341 aataaacaga tttttaggtt tagttaaaac attagaaaaa aatgatgtaa catcgattaa
     2401 tcgattaaag cactgggtat ctctaacaaa agaattgtct aatccatctt tgaagaggga
     2461 aatagaatat tctcagtcag aggatttatt aaaagcttta aaatggtcgg aagaagtcaa
     2521 tcaacagatt agtcaagcaa aaggattaga taaacccttt ttaagtgcta gagatactat
     2581 aaaagctctg aaaaaatatg ggaagataat tattgtaagc tcagctaata aggaagctgt
     2641 tcaagaagaa tgggaaagac atgaattatt atcattagtt gatgaactgt gttgtcaaga
     2701 taaaggaaaa aaagaagata ttattcgaag tgtaattgaa aatggttgtg atttggataa
     2761 gatattaatg attggtgatt ctcctggtga tctagaagct gcaaataaaa ataaagtttt
     2821 cttttatcct attttggtca ataaagaaaa agaatcatgg gaaaacttaa gaactgatgt
     2881 t
//
LOCUS       DAYYKN010000212         2858 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000212 DAYYKN010000000
VERSION     DAYYKN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.217"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(887..2227)
                     /gene="glnA"
                     /locus_tag="ACHBB7_002816"
     CDS             complement(887..2227)
                     /gene="glnA"
                     /locus_tag="ACHBB7_002816"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM9210698.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(2255..2647)
                     /locus_tag="ACHBB7_002817"
     CDS             complement(2255..2647)
                     /locus_tag="ACHBB7_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9210699.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          865 a          611 c          460 g          922 t
ORIGIN      
        1 caaatcagaa aatcgttact gaaaaatttt aagtgaaata atagataaaa aaataaaaca
       61 aacattatga tagataatat cccacctaac aaagaactac tttttcctac ttgcagcaac
      121 agtttatata gcttatcgta gaaacaatct ttttctttca cccttattta cctcctttac
      181 gtaaaaaacg tagtccacag ttctcggatt tttggcatat cttttctatt aaataatatc
      241 aaaaacaaaa caattccact aattttttca aagatgacct ttttttcaac aaaagtgcct
      301 tgtagggatt cattgaaggt aacgaatcac ataagtaaaa aataaggaga tgcgacaaaa
      361 gtcgtgcctc cttatttcta aataaacggt gttctcctgc caactcctcg gtcacaagtc
      421 acaatatcca tcaaacatga gaagctgttt ttgaaattat ttcttgtgat tcggagttaa
      481 atggtaggct cacaacctct ggataaacgg tattcaaaag ccagtttctc ggtattaagc
      541 caaatttatg caaaaatgta gaaagccatt ttcacaaaat ttttcttaat actcgaaact
      601 aaacgtcttt ttcaaaacct cttggtaaac ggtgttcaaa agcttgcatc tgcggtaata
      661 agttcagcca attcaaaaac aagaaaagct gtttttgctt ggctattctt attacttgtt
      721 gcagggcagc tttttcacaa cctctccact aaacggtgtt caatcagctg accctcggta
      781 ttagctcaaa aaacggcaaa atatgaggaa ctatttttcc gttagtttct ctaatactca
      841 ggtcaaaacg ctgatttcac agcctctaat tcacggtatt cctgttttaa taaagttcta
      901 gatattgttc tctttcccac tcggaaacag tttgtctgaa tgccgcccat tcgagacgtt
      961 ttgcttcaac aaaatttgag aagatatgtt gtcctaatgc gtcaatcatt acattatcct
     1021 tacgcaattc tttgatagca ttatggatag ttgaaggtaa gtcctggata tgtgcagctt
     1081 ctctttcttc ctcatccatt acataaatgt tacggtcaac agctgctggc ggtgtgatct
     1141 gattacggat accgtctagt cctgcttgca acaatactgc cattgccaag tatgggtttg
     1201 cagatggatc aactgaccgc aattccaaac gtgtggataa accgcgagat tctggtacac
     1261 gtactaatgg cgaacgatta cggccactcc atgcgacata aacaggcgct tcataacctg
     1321 gaacgagacg tttataagaa ttgactgttg gattgcaaac ggctgtgtag gcacgagcat
     1381 gtttcagcaa accacctaag aaatgataag ctgtttcact tagttccaat tcaccgtctt
     1441 tatcatagaa aacatttcct tcatcattga acaaagacat attgcaatgc ataccagaac
     1501 cattgatccc aaataacggt ttcggcatga aagtcgcgtg aagtccatgc ttgcgtgcaa
     1561 tcgttttgac aacgagcttg aaggtttgga tattatcaca agcatctacc acatctgcat
     1621 atttaaagtc aatttcatgc tgtcctggcg ctacttcatg atgtgaagct tccacttcaa
     1681 agcctagact ttctaattcc aacacgatgt cacggcggca attttcacct aaatctgttg
     1741 gtgcaaaatc aaaataacca cctttatcat tcaaggtcgt tgtgatccca ccgtcttcgt
     1801 ctaatttaaa taagaagaat tctggttcag gtcctaaatt gaaagaggta aaaccaaggt
     1861 cgcgcatatc tttcaatgct cgtttcaaat tcccgcgagg atcgccggca aatggtgtcc
     1921 catccggatt atagatatca caaattaaac gagcaacttt tccatgagca ctttcccacg
     1981 ggaagatcat ccaagtggag agatctggat acaaatacat atcgctttct tcaattcgaa
     2041 caaatccttc aatcgaagaa ccgtcaaaca tcattttatt ttctaatact ttgtcgatct
     2101 ggctcactgg aacttctaca tttttgatcg tacctaagat gtctgtgaac atcaatcgta
     2161 aaaatcgtac gttttcttcc tcaatgattc gtttgatttc ttcacctgtt atctgtttct
     2221 ttaccatggt atccctcttc cttttttaat ttattcagtc aaagaattgt ctttgcaatc
     2281 cttgcgactg ataatgacct gttttgctta acccaccttg cgccaaaatc tcatcacgaa
     2341 aaatttgtcg aacgtcatga tctgtcaacg gcttcttgtt ttttgcttct tgttttttcg
     2401 cctgtgccat ttcgtataca cgtttgatcc cagccatatt caatccctct gaaagatagt
     2461 ctttaatctc aagcaacata tcaatatcat tcagcgaata catacgtcgg tttccttcgc
     2521 ttcgttccgg atgaactaaa tcttgttctt cataatagcg aatttgccga gcagacaaat
     2581 cagtcagctt cataactgta ccaattggaa atacggacat agatcggcgc aattcctttt
     2641 ctcccatcag acgtcccctt tcgttttata agttggctaa agtgtaccaa ttaaaaaaaa
     2701 tattgtcaac tgcatatgtt aggttttcta acatgagatc tctatttttt ataaaataac
     2761 aaaaaaataa gaacacaaaa aaggtatcta gttgattttc gtaccagata ccttttattt
     2821 tttattatac tgtactatcc tttattagtg tttttatt
//
LOCUS       DAYYKN010000213         2796 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000213 DAYYKN010000000
VERSION     DAYYKN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.218"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACHBB7_002818"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACHBB7_002818"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM9210700.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1085 a          435 c          569 g          707 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattgg
//
LOCUS       DAYYKN010000214         2773 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000214 DAYYKN010000000
VERSION     DAYYKN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.219"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..562
                     /locus_tag="ACHBB7_002819"
     CDS             116..562
                     /locus_tag="ACHBB7_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9210701.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            835..2187
                     /locus_tag="ACHBB7_002820"
     CDS             835..2187
                     /locus_tag="ACHBB7_002820"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM9210702.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2358..2645
                     /locus_tag="ACHBB7_002821"
     CDS             2358..2645
                     /locus_tag="ACHBB7_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9210703.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          975 a          453 c          546 g          799 t
ORIGIN      
        1 ttttacgtct tttttcatta aaatacaata aaattgaatt ttcttttcat gttaagtgga
       61 ttttaaaatg taaaaatagt atgataagac gagaataaca acgtggggtg tttacatgga
      121 aaataagtta gtcgaagagg ttatcgaaaa gttaaaaaaa gagaacattc gaatcacacc
      181 tcaacggtac gcggttttag aatatttgat tgaacatcat tcccatccaa cggctgatga
      241 gatttataaa gcacttgaac atcggtttcc aaatatgagt gttgcgactg tttacaataa
      301 ccttcgctta tttacttcga ttggatttgt tcaagaaatg aagtacggcg atgcttctag
      361 ccgatttgat ttcagttcaa agcgtcatta tcatgtaatt tgtgaaaact gcggtaagat
      421 tgttgatttt cattacccag ggcttgaaga tatagaaatg gctgcaagca gactgactga
      481 ttttgagatt aatgaacatc ggttggaatt gtatggactc tgtccggatt gtaaagagtc
      541 agcaaaaaag gcgcaaatgt aaaaacagtt tctacaagtg ttatacctaa aaaaagctag
      601 ctgtgtaaaa aaaacgctag cttttttatt tttttgtctt ttattcacaa tcttagatat
      661 aaaagtgaat attattaaaa taagaatctt gtagatattg ggttcttatg cttgaaaagg
      721 aatgaaacta caaacaaacc tttgtgaatt cacaagcatt aaactgttgt atccgtttta
      781 tttctgttat agtagttttg tcgaaggaca aaatgttaat cggagggacc ataaatgaaa
      841 gtagtagtaa ttggctgtac tcatgcaggt actgcagctg taaaaagtat tttaaaaaat
      901 catccagaag cagaagtgac agtatatgaa agaaatgaca atatttcttt cttatcttgc
      961 ggtatcgcac tttatgtcgg cggagtagta aaggacccag caggcttgtt ctactcaaat
     1021 cctgaagaat tatcttcatt aggagcaaca gttaaaatgg aacacgatgt aacagacgtc
     1081 gatacagaaa ataaaacagt aacggcaaaa gatctgaaaa caggagaaac agaaacggtt
     1141 tcttatgata aattagtgat gacaactggt tcatggccta ttatcccacc gattagtgga
     1201 atcgatgcga aaaatgtact cctatgcaaa aactacaatc aagcaaatga aattattgca
     1261 caagcgaaag aagcaaaacg tgtagtaatt gttggtggtg gatatatcgg tattgaatta
     1321 gtagaagctt ttgtcgaatc tggaaaacaa gtgacattga ttgatggatt agatcgcatc
     1381 ttgaataaat atctagataa accattcaca gatatcttag aaaaagaatt gacagaccgc
     1441 ggtgttactc ttgcgctagg cgaaaatgtt actgaattta tcacagatga agaaggaaaa
     1501 gtgaaacaag tagctacacc aacagataca tttgatacag atatggtcat tttatgtgtg
     1561 ggcttccgtc caaatacaaa actgatcgaa ggaaaagtag atacactgcc aaacggggca
     1621 atcaaagtga atgaatacat gcagacaagt aatccagaca tttttgcagc tggagactct
     1681 gcagtagtca actacaatcc aagtggcacg caaaactata ttcctttggc aacaaatgcc
     1741 gttcgtcaag gtttgcttgt gggaaataac ttgacagatc ataaaatggc gtatcgtggt
     1801 actcaaggaa cttcaggact ttacctattt ggctggacga tcggttcgac aggtgtgacc
     1861 aaagaaagcg caactttgaa cgatctagaa gtccaagcga ctgtatttga agataattat
     1921 cgtccagagt tcatgccaac tactgaaaaa gtaatgatgg agctagtcta tgaaaaagga
     1981 acgaatcgta tcgtaggtgc tcagttcatg tccaaatacg acattacaca atcagcaaat
     2041 accatgtctt tagctgttca aaataaaatg actgtagaag atctcgcact ttcagatttc
     2101 ttcttccaac cacattttga ccgcccatgg aattacttga acctacttgc acaagcagcg
     2161 ctaggcgaaa tcgaaactgc acaataaaga aaaagggttg tgacacaatt ttgtcacgac
     2221 cctctgtctt tttttgtgaa aagtacaatt agttgtcttt cttcgaattt tttgtcacaa
     2281 taagcagcaa agataaagct gttacataaa agaacaagca gctttatttg attttgaaaa
     2341 gaaaggtgag aaagaagatg aataaagaat attttgtcca acaagatgca tttttagaag
     2401 attataaaag tgcttcgaag aaattaacga tcaatattga tgagttggca gatcaagtac
     2461 tctctcagtt gaatgaaacg caagaggaat attacacgat gccagcaaat gacgccaaag
     2521 ataatgtttc tcatcgtttt cctttcagta gacgcatcta ctcagatgaa aatgacggca
     2581 cattgaatac agagtacaca tatgaaggtc agccatatgt gctagaaact gatgatgatc
     2641 tttaaagaca gtttttgaaa aggccgctca tccttaagtg atgacaaaaa atcatcacaa
     2701 ttaccgatga ctgtctcttt actatctttt aatcagaaaa aggggttatg acaagctttg
     2761 tcaacccctt ttt
//
LOCUS       DAYYKN010000215         2739 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000215 DAYYKN010000000
VERSION     DAYYKN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.221"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHBB7_002822"
     CDS             <1..156
                     /locus_tag="ACHBB7_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM9210704.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="ACHBB7_002823"
     CDS             175..1452
                     /locus_tag="ACHBB7_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9210705.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="ACHBB7_002824"
     CDS             1462..2118
                     /locus_tag="ACHBB7_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9210706.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..>2739
                     /locus_tag="ACHBB7_002825"
     CDS             2118..>2739
                     /locus_tag="ACHBB7_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9210707.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGL"
BASE COUNT         1162 a          252 c          469 g          856 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctat
//
LOCUS       DAYYKN010000216         2733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000216 DAYYKN010000000
VERSION     DAYYKN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.222"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..1488
                     /locus_tag="ACHBB7_002826"
     CDS             10..1488
                     /locus_tag="ACHBB7_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM9210708.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(1556..2647)
                     /locus_tag="ACHBB7_002827"
     CDS             complement(1556..2647)
                     /locus_tag="ACHBB7_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM9210709.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT          950 a          496 c          440 g          847 t
ORIGIN      
        1 gagtgcatca tgaaaaagtt atctcaaaaa cgtttagctc aaatcattat tgattctaga
       61 aaatctctga aaatgacaca acaacaactt gcagtagcta ctggtatcaa ccgtgcctta
      121 attagtcgat tagaatctct cgattttatc ccgtcaatac aacaattaga atcattagca
      181 gaagttctaa attttgaacc aacagatcta tttataaatg aatcaaataa atcaaacaga
      241 acaaaacatc agtcgaagaa tattgctgta gccggtactg gttacgtagg tctttccatt
      301 gctactcttc ttgcccagca caatcaggta acagcagtag acattcttcc tgaaaaagta
      361 aaacaaatta acaataagaa atctccaata caagacgatt acattgaaaa atatctagct
      421 gaaaaaaatc ttaatttaat tgcaacgact gatggtgcag ccgcctatag agaagccgat
      481 tttgttgtca ttgctgctcc aacaaattat gatagtaaac aaaattattt tgatacttct
      541 gctgtagagt cagtaatcaa attggtgtta gaagtcaatc caaaggcaat aatggtcatc
      601 aagtcgacgg tacccgtagg ctataccgaa agtattcgta aaaaatacaa tactaaaaat
      661 attctattca gcccagaatt tctcagagaa tctaaggcat tatatgacaa tctatatcct
      721 tcgcgaatta ttgtcggcac ggaccaaact gacaaaactt tacgagaagc ctctcacact
      781 tttgcagatt tactccaaga aggagcaatc aaaaaagata tcgaaactct tttcatggga
      841 ttcacagaag ctgaagcagt taaattattc gctaatactt accttgcact tcgagtatct
      901 tacttcaatg agctggatac ttatgcagaa atgaaaggac taaatacaca agaaatcatt
      961 cacggggttt gcttagatcc aagaattggc acacattata ataatccatc ttttggatat
     1021 ggaggctatt gtttaccgaa agatacaaaa caacttctca ctaactataa cgatgttcct
     1081 cagaatatga taagtgcaat tgtcgatagt aatcgtacaa gaaaagactt tatagcagat
     1141 cgagtgttag aacttgctgg cacgtatgag gctaacagtt cgtggaatgc agaaaaagaa
     1201 aaagaggtag tcataggtgt ataccgtctt acaatgaaga gtaattcaga caacttccgt
     1261 cagtcatcta ttcaaggagt tatgaagcga attaaagcaa agggggcaac tattgtaatt
     1321 tatgaaccaa cacttgaaaa taacactaca ttttttggtt caaaagtaat aaataattta
     1381 gatgatttta aaaatatcag tcacagtatt attgctaatc gttacgatag taatcttgat
     1441 gatgtaaaag aaaaagtata taccagagat ttatttcaac gagattaaaa aataacacct
     1501 cagatttgta gtgtctgagg tgtttctata aacttttata agtaaaaaaa tacaattata
     1561 cttcataaaa agacttatac cactgcgcaa atttacgaag cccgtcacgc aaacttgtac
     1621 ttggtttaaa ttcaaaatct cgttctaacg cactagtatc tgcataagta actggcacat
     1681 ctcctggttg cattggaact agttcggtat gagattcaaa gtcgtaatct tctggtagca
     1741 cccctgctgc aattagctct tgttgtagaa tatctacaaa atctagtagg ttttccggcg
     1801 tattgttacc aatattataa atatgatatg gtgggattgg tagcccatct tccccatcaa
     1861 ttttctctgg agcgcactgc ataaccctct ttactccttc aacaatatca tctatatagg
     1921 taaagtcacg cttgcaattc ccataattga atatttggat attatcacca gcacgaagtt
     1981 tatcagtaaa accaaaataa gccatatctg gacgcccagc aggaccatat actgtaaaga
     2041 aacgtaaccc tgtagacgga atattgtaaa gtttagaata tgcatgtgcc attgattcat
     2101 tacttttctt agttgctgca tacaacgata cttgattgtc tactttatcc tctgtagaat
     2161 aaggaatttt cttattagta ccataaacgg aagacgatga ggcatacact aaatgttcca
     2221 caccagattc tccattatca tatgaatgac gacaagcttc aagaatatta tagaatccta
     2281 ttatgttaga ttcgatatac atatctggat tagtaattga ataacggact cctgcctgag
     2341 ctgctaaatt caccacgata cttggtttat aattatcaaa gatgaaatca attgttgctt
     2401 tatcagcaat agaacctttg ataaaattcc aagtagatcc tggatggttc tttgcctccg
     2461 tttctatctc tgataatcta tagttcttaa tagagacatc atagtaatca ttcatattgt
     2521 caatgccaat gatttttatt gatgactgag tacgtaataa ctcaagaacc aaatttgatc
     2581 caataaaacc ggctgcgcca gttattaata ttgttctatt tgataaggac gtgtttaatt
     2641 ccagcatttt ttagcactca ctttcttttt ttcttatgaa tacatatttg ttatatatta
     2701 aaaaatctta tttagactta tctattgtca ctt
//
LOCUS       DAYYKN010000217         2678 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000217 DAYYKN010000000
VERSION     DAYYKN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.223"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..213)
                     /locus_tag="ACHBB7_002828"
     CDS             complement(<1..213)
                     /locus_tag="ACHBB7_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9210710.1"
                     /translation="MKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVPAFIIMMLI
                     DELVLENVKPPWLYFTVSFVTLLFMYW"
     gene            complement(197..1951)
                     /locus_tag="ACHBB7_002829"
     CDS             complement(197..1951)
                     /locus_tag="ACHBB7_002829"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9210711.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(2061..2630)
                     /locus_tag="ACHBB7_002830"
     CDS             complement(2061..2630)
                     /locus_tag="ACHBB7_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM9210712.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT          928 a          505 c          363 g          882 t
ORIGIN      
        1 ccaatacatg aaaagcaaag taacgaacga tactgtaaaa taaagccaag gcggctttac
       61 attttctaaa actaattcgt ctatcagcat cattataatg aatgctggta ccatattaat
      121 gcagtataca aaaaagctag aaatagatgc tttaaccaaa tcttctgcgc cctgattagt
      181 taaagcgaat ttgtttttat aaaaactttt cattgtcaag cctccaatca tttgcattct
      241 cataattagt gaccaattgt ttatacactt gatgtttagc cattaattca ttatgatttc
      301 cacgttctac tattttccct tctgacaaga caagaatttc atctacagat ttaatgctgg
      361 ataatcgatg agctatcata ataactgttt tttctttcat cagatgttga aaagcctttt
      421 gtagttcata ctcattatct ggatcaatag atgcactggc ttcatccata ataacaatct
      481 ttgcatcttt taaaattgct ctggcaatag ctatacgttg tttttctcct cctgacagat
      541 aaactccttt actaccaatt aaggtatgct ctctttcggg aaatttttct aaaatcgatt
      601 cgcaaccggc taatcttagc gcgtcaaata ctttacttgc tggcgcttga ggatttgcta
      661 tagaaacgtt atcgtaaata ctctgattga atagtttcgt atcttgaaag acaaaagcaa
      721 ttgtgttaat aattgtgtct tttgaatagt gatttaaagg cttaccacca attttaatct
      781 gtcccttatc cacattgtag aaacctgaaa ttagtttagc tatcgtactc tttccgcttc
      841 ctgattttcc tactaatgca taaattcttt tttgttctaa aactaagttg aaattttcta
      901 aaacacactc ttctttgtat gaaaagctga cattttcaaa tgcaatgtca caatttttaa
      961 tctgtgtctc gttcccatat tttagttggt cttgcttcat ttctaaataa agggagtcta
     1021 gtgtttcgac tgcatatctt gcttcaaaca agtgcatact tacatacatt attctcatga
     1081 aagaaacaaa aataattcca cttaaaaaaa acaacatcaa caagtcaaca gctaatatct
     1141 tgggagtggt aagagaagtt ataaaaaagg ttataggaac tatcaaaatg caaactatgc
     1201 caaaaaatag ccattgaaaa agaacaaaag gtgttttccc actcaaagaa tacttatatg
     1261 caaacttagc ataatcgtta attgctttat tcaaggcttt aaatgatgtt actttagcac
     1321 caaatatctt tataacttga attcctcgga tatattctac cgtttcagag cttaacacat
     1381 ctaacgattc ttgatatttt tgaataaaat ttgtattctc tgtcattttc tttaacaaga
     1441 atacaccaat tactaacatg gcgattagaa ccaatccaat tttaaaatta atgaaaaagc
     1501 ctaaggcaac tgataaaaca ggtgttacta tggcttgagc actatctgga atcaagtgag
     1561 caattgccat atgtgtttta gccgcgttgt catcaattgt ctttcgaata tatcccgaag
     1621 aatttaaatc aaaaaaacga aaacttgctt ctgttaaacc gctaattccc ttttttctca
     1681 aattagtctc taagcgaaac ccaaggatat gagaaaataa gccagaaaaa atataaaata
     1741 aagctcctaa agttaaccaa gcaacagttt gaaccgcata actcttagct aaatattcat
     1801 ctcctacaat aattagagac ttaagaaatt tgtaaatact gtaatatcca tagactgtta
     1861 atacgacaga aaaatcagaa aataaagtgg ctaaccatcc gtagtatttc attttaggga
     1921 cataactaaa taattttttg tatactttca ttttttcctc ccccgcattc aatttcttct
     1981 cttattcgtt tacaactgtt aatgctttcc gtaatagcgt aaattagaga tgtacaaaca
     2041 gatagaaggt aaactacgtt ctattttttg tattttttta agacttttct tccaatcaat
     2101 acaccaatca tagataaact tgctacaact aacactgaaa tgattcccca tatacctacc
     2161 gcatagtgga tcattgcatc cattgttgca gcagattgtc ctttatctat ccaatcttga
     2221 cgaaaacgat ctgtaaagaa ccatatagga atccactgtc cggcagcact gcctaattgc
     2281 attagagccc aagacgcaac ttgaggaatt gctcgatcat atccaaatac agatagaata
     2341 atatcagcta agatcgccat actaatcgaa ctgataaacc aaggtaagta cgccccgccc
     2401 attaacgtaa aaaataacaa taaaacaatt gtgtaaatag taaaaattcc ttttctaggg
     2461 tagttatagc taatataaac aaatatgatt ccagataata atccaaaaat ccctggggat
     2521 atgctgtgac caaacgcccc accaatacta ctaaccatta ttgaaaaaaa gtaaattact
     2581 agggaaataa ctgtagctaa accaataaaa atataatctt ttgttttcat atatattctc
     2641 ctcaattcta tttcgttatg tcattaaaaa tctgttct
//
LOCUS       DAYYKN010000218         2672 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000218 DAYYKN010000000
VERSION     DAYYKN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.224"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..1128)
                     /locus_tag="ACHBB7_002831"
     CDS             complement(34..1128)
                     /locus_tag="ACHBB7_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM9210713.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1317..2078
                     /locus_tag="ACHBB7_002832"
     CDS             1317..2078
                     /locus_tag="ACHBB7_002832"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9210714.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2193..2633)
                     /gene="fabZ"
                     /locus_tag="ACHBB7_002833"
     CDS             complement(2193..2633)
                     /gene="fabZ"
                     /locus_tag="ACHBB7_002833"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM9210715.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          833 a          538 c          459 g          842 t
ORIGIN      
        1 ttctagcgga ttatttctgc aataactaat tatctatttt ttattttcaa ttaatttaaa
       61 gcttgaccat ggtttcaaaa agtcaaggag gaataattcg tcatcggaga ttcttcctac
      121 aacattgaca cgtccgtcgt ttttcatatc cttcaaggcg atttgtgttt ctcctttgta
      181 ttgaccgtac ccttcattgt caatcagtac atccccatga tgcatatctc gcgtattatg
      241 aggcgggaaa tctttatctt tgtagtatac acgagtcatc gttgaacgaa ggatatattc
      301 tgagcgatcc cctcgataac tatgaaggtt atcaaacaag cagattcgtt cgttttctgt
      361 aataccttct tccgtatcta ctttcaatgt tggataatcc gcgttaaatg cttctgccat
      421 ttctttcaat tcttcttcac ttgcgtacgc atttccaata gaaatatcat caatcaatcc
      481 tgtaaggatt aaatgcttca cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt
      541 gcacaagcca tcttgcgtag gccaagggcc aaactcagca gattgcgaat tgacgaatgc
      601 catcgtattc aaattatatt ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc
      661 taaaccagag tatcgatgag gataaaaatt atgagaacct aataaattat ccgtgtttgg
      721 cgaataactc atgatgttgt caacgtaatt cgtgcccgaa ctcatattga tctcgatttt
      781 gataccaaaa ggattgcgcg tcatttttgc ttcttctgct cctgtgaatc cgatatctaa
      841 gcggaccccg taggcaccca tcttatggaa aaaagataag tcatcgtaag aaatctccaa
      901 ctgttcaaat aacgctggat tgatatcaac cattacttcc atgcctaggc tattcgcgta
      961 atcaactact tctttaaatt ctgaaagaac tttttcttta tcatcattga tctgcagcaa
     1021 actggtaaaa acccgtttat acccatactt atgagctaaa tccaagtacg ctttgtcctt
     1081 ttcaaatgtt gaccgttctg gataaatcga gattcctaat tttcccatat taccatttcc
     1141 tcctgcatct tttcgttttt gtatccactt acattatagc aaacacaaag taaatggtct
     1201 acaccttttc tttttctttt aaaataaatg ataaattcca ataatttaag taggtatttt
     1261 ccctctattt tgttatcatt aaagataaca aaatagtaaa ttggaaagga acagttatga
     1321 agaaaaataa agttatttta agtattagtt tagcagcagc attgctgttt gctgctggtt
     1381 gttctgcaaa caacacgact tcttctagtt cgtctacaga aagttcaaaa agtacatcta
     1441 ctgcggaaag cacgactgcc agtagcgtcg atttgaacgc attggaatta ccacaattga
     1501 cgaaagatgt cgccgaaaat gaagatctgg tcgaaatggt gacgaataaa ggcacaatca
     1561 aaatcaaatt atttcccgaa cacgcaccaa aagcggttga aaactttatg acacatgcaa
     1621 aagacggtta ctacaacgga ctgactttcc atcgcgtcat caaagatttc atgatccaag
     1681 gtggcgatcc taaaggcgat ggcactggag gggaaagcat ttggggagaa ggatttgaaa
     1741 cagagatcaa caaccacctt tataatataa gaggtgcgtt atctatggct cgctcacaag
     1801 atcctaacag taacggcagc cagttcttta tcgttcaaaa tacagatgat atgcatgatg
     1861 gtcttttaaa agacgactat cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc
     1921 cttcactaga tggccattat actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg
     1981 atgctattgc aaatatcgaa acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg
     2041 ataaaatcaa tatactgcaa gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc
     2101 atagatgaca acgtatgcta ggattcaggc gtcttctttt tacataaaaa gcaagtaagc
     2161 ttccttttgt tttgctatat caggttttat agtcaagcat tttggttgac gatgaacgtg
     2221 aactcgcact cacagacttt tttatcatct acatatgctg cagataacgc tgtaccgact
     2281 gatcccttca ttttggtgat ttcaaaatga catttcagca catctcctgg aacaactttt
     2341 tgacggaatt tggccttatt gatcccaccg atataagctc tttttcctag aaactcttct
     2401 gattttagga taagtatcga acctgcttgt gccagtgttt ctagaatcaa cgcacccggc
     2461 atcactggat tgtcaggaaa gtgaccttgg aaaaaatctt cattgattgt tacatttttt
     2521 gtcgcaacga tcttttttcc tggttctaaa gtatctacat aatcaatata acaaatagga
     2581 taacgattag gaatcaattc cattacttca gtagcagata atacgtaaga cataattaaa
     2641 ctccttcttt caggcaattg ccattttcat ta
//
LOCUS       DAYYKN010000219         2660 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000219 DAYYKN010000000
VERSION     DAYYKN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.225"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACHBB7_002834"
     CDS             complement(138..428)
                     /locus_tag="ACHBB7_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210716.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACHBB7_002835"
     CDS             complement(546..806)
                     /locus_tag="ACHBB7_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210717.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACHBB7_002836"
     CDS             complement(943..1356)
                     /locus_tag="ACHBB7_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM9210718.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACHBB7_002837"
     CDS             complement(1812..2297)
                     /locus_tag="ACHBB7_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210719.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACHBB7_002838"
     CDS             complement(2436..2651)
                     /locus_tag="ACHBB7_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM9210720.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
BASE COUNT          983 a          441 c          409 g          827 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct
//
LOCUS       DAYYKN010000220         2654 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000220 DAYYKN010000000
VERSION     DAYYKN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.226"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..713)
                     /locus_tag="ACHBB7_002839"
     CDS             complement(147..713)
                     /locus_tag="ACHBB7_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9210721.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(713..1669)
                     /locus_tag="ACHBB7_002840"
     CDS             complement(713..1669)
                     /locus_tag="ACHBB7_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM9210722.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1669..2325)
                     /locus_tag="ACHBB7_002841"
     CDS             complement(1669..2325)
                     /locus_tag="ACHBB7_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM9210723.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          823 a          535 c          487 g          809 t
ORIGIN      
        1 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
       61 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      121 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      181 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      241 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      301 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      361 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      421 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
      481 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
      541 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
      601 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
      661 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
      721 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
      781 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
      841 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
      901 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
      961 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1021 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1081 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1141 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1201 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1261 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1321 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1381 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1441 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     1501 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     1561 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     1621 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     1681 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     1741 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     1801 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     1861 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     1921 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     1981 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2041 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2101 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2161 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2221 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2281 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2341 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2401 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     2461 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     2521 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     2581 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     2641 ttaagagaag ttat
//
LOCUS       DAYYKN010000221         2622 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000221 DAYYKN010000000
VERSION     DAYYKN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.227"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACHBB7_002842"
     CDS             <1..435
                     /locus_tag="ACHBB7_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM9210724.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACHBB7_002843"
     tRNA            complement(759..842)
                     /locus_tag="ACHBB7_002843"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACHBB7_002844"
     CDS             1370..1870
                     /locus_tag="ACHBB7_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9210725.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2284)
                     /locus_tag="ACHBB7_002845"
                     /pseudo
     CDS             complement(2060..2284)
                     /locus_tag="ACHBB7_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
     gene            complement(2370..>2622)
                     /locus_tag="ACHBB7_002846"
     CDS             complement(2370..>2622)
                     /locus_tag="ACHBB7_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9210726.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          815 a          497 c          457 g          853 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaggctctt cgtcaaattg tgttggcgaa gacaaatttg aataaaactt agtccgatat
     2341 gcggcgcgtt ctgtgcgccg catatttttt tagttttcaa agatcagacc ttgttgtaaa
     2401 tagatccgtg cccgaaagtt ataaaagttg cgataaccaa acgcaattcg tttaatcagc
     2461 ttgatcttgt tattcagccc ctcagtgacg ccgtttgagt ggggcacctt aaaggcattt
     2521 gaaatacccg attggtagcg atggaagatc gtgaatttct ttttaaacga ctgaggtaac
     2581 gtagatgaaa ttgttttaag ggtttcttga aagccttccg ta
//
LOCUS       DAYYKN010000222         2578 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000222 DAYYKN010000000
VERSION     DAYYKN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.228"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..2353
                     /locus_tag="ACHBB7_002847"
     CDS             200..2353
                     /locus_tag="ACHBB7_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM9210727.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          884 a          444 c          564 g          686 t
ORIGIN      
        1 acataatttt caaaaataag tttatttttt aatgaaaaaa atagttggat atgtaacttt
       61 aagtgattgc tgtgaaacaa aaagttgatt ttatcttgaa attaggtaaa gaatttcttg
      121 ctccgcccat tttttctttt ttattaagaa gtttttgggt acaatgaagc atgaatgtaa
      181 ataggcggaa ggataggaaa tgatgaaaga ttttatgaaa aaaatattaa atacttcttg
      241 gttagaaaaa ataagaaaag gtgcacaaga acctaagaac aaaacgtttc gccggtccca
      301 tgttcctttt cgcttaaatt ttcttttttt cattatattt acgttatttg ttgcgttgat
      361 tgctagattg ggttatttgc agatcgtgaa cggcgaagag atggaggcgc gagtgaagtc
      421 tacttctacg attacggtcc aagaaagtac tcctcgcgga atgatctacg actccacagg
      481 aaaagcttta gttacgaata aagcgaatca ggccatcaca tatacgagag gaacacaagt
      541 tacggctaaa gatttgcttg aaatcgcaac gaagctgaac tcgctgatcg atgttccggt
      601 agatgaaaat ctgacagata gagataaaaa agatttttgg ctggctgatc ccaaacattt
      661 gaaagaagcc acaaaaagac tttcggcaaa agaaaagcaa ttagggaaca gcgaacaata
      721 tagtgcgatg gtcaataaag tcacagctga tgagattaat ttaaacgagg agcaattaaa
      781 aattgctacc atctttaaac ggatgaatag tgcatatgaa ttaaacactg tctatatcaa
      841 aaactctggt gttactgatc aagagttagc tgttgtggca gaacgcgctt ctgaacttcc
      901 aggagtttcg actggcacag actggacacg agaatataat gcagcaagtt cactgaaaag
      961 tattcttggc agtgtgacga ctgaaaagca aggacttcca gcagatgaag cagagaaata
     1021 cctagcaaaa ggttattctc ggaatgaccg tgtaggacaa agttatttgg aaaaacaata
     1081 tgaagatgtc ctgcaaggaa ccaaaaccca atatgaagtt tcccttgata atgaaggaaa
     1141 tgtttcgaat caaaaagaaa tcttctctgg ggaaaaaggt tctaatttaa tgttatctat
     1201 gaatgcagaa ttccaatcaa aagtagaaga aattttgaaa aggaattatc aaacattgat
     1261 caacaacggc aaagcacagt attcgcctgg tgcttatgca gtggctatga atcctcagac
     1321 aggagaagtg ctagcaatga ctggtttcag tcatgaacaa ggttccaaag aaatcaccga
     1381 gaatgcttta ggcacgatta ctagcgcctt tgatccaggg tctgtagtca aggctggtac
     1441 tttgacagca ggctgggcaa gtaacgcgat cagcggaaat caagttttga ttgatgagcc
     1501 gattcgtctg caaggtgctt cagaaaaaag ctctgtcttt aatcgaagcg gacaagttgc
     1561 tcttgatgca gtcaaagcat cggaattgtc ttctaatacg tatatgatca aagtggcgtt
     1621 aaagatgtta ggactagact atacacctgg aatgggacta cctagtttag atgaagaagc
     1681 taaagcttat cagcaattga gagactcttt caaagagttt ggtcttggta caacgactgg
     1741 gatcgatttg ccgaatgaat ctcctgggat ctcacgttct gttgattata tgaagaagtt
     1801 caatgcggat aatggcaaag aatggtatgc tccaggtaac tttaccgatt tggcatttgg
     1861 acagtttgac acatatacac caatccaatt agctcaatat gcttcaacag tcgctaatgg
     1921 cgggaaacga gtacagccgc acctagtcaa agcgatctat ggcaatgacg aaaatggaaa
     1981 cttgggtgaa gtgaagaaag aaattggcac aacagtagaa aatacggtca atatttctgc
     2041 agaaaatatg gcgatcctcc gtgaaggatt ccaccaagtt gtccatggta ccgatcctta
     2101 cacaacagcc aaaccattag ccagtgcgaa aatggacctt tccgcaaaaa cagggactgc
     2161 agaaacggtt gcggaaggtc atccagatat tgcgacagtc aatagtaata tcgtggcata
     2221 cggaccaaca gacaatccgg aaattgccat cagtgttgtc ttgccgaact tattagacga
     2281 acaagaccac ataaacttga cgattgctaa agaaataatg gacgcgtatt atgatatgtt
     2341 catggcaaaa taaaaaagag gtcgtgaaaa agccgcttag tttcgagtat gaagaaaaat
     2401 tttgtgaaaa tagcttctca catttttgct taaatttgac ttcataccga gaaactggct
     2461 tttgaacacc gtttactcgg caaaaaaggt tgtgacaaaa attgtggctc tttgtcaata
     2521 aggactgatg agttgtgcaa aatcaaatct gagtcagaat gaaccacatt ctggctca
//
LOCUS       DAYYKN010000223         2568 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000223 DAYYKN010000000
VERSION     DAYYKN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.229"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            227..979
                     /locus_tag="ACHBB7_002848"
     CDS             227..979
                     /locus_tag="ACHBB7_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9210728.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1119..2042)
                     /locus_tag="ACHBB7_002849"
     CDS             complement(1119..2042)
                     /locus_tag="ACHBB7_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9210729.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2057..2329)
                     /locus_tag="ACHBB7_002850"
     CDS             complement(2057..2329)
                     /locus_tag="ACHBB7_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210730.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT          857 a          493 c          416 g          802 t
ORIGIN      
        1 tcatctcatt cctcttttct atatagtcga tttagatcca atcttaagtt atgttaccat
       61 ctttcctagc attaaggaat cggttacaat tgaaccttta aagacaaaag gatttacctt
      121 ttatcactta atagtataac ataatttcat gagaaaaaaa tacaattttt tcttgaccat
      181 ctcgttacgt aaaggattat gcttacattt gtcaggagga aaaataatgg agtacacaat
      241 aaagaaaatg tcagagatat caggagtcag tccgagaacc ttgcgctttt atgatgagat
      301 tggactgcta aaaccagcta ggatcaattc ttcaggttat cggatttatg gcaaaaaaga
      361 agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg gcattcaagt tggatgatat
      421 ccaagaggtc ttagacaatc catcttttga ccaccagaaa gctttaatca agcatcagca
      481 aatgttgctg gaaaaacggg cacaaatcga taccctcttg acaactgtcc aacagacact
      541 tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca
      601 acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa
      661 aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga
      721 aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga
      781 agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttat cttacagctg
      841 gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg
      901 gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat
      961 tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc
     1021 taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc
     1081 attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa
     1141 ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa
     1201 gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg
     1261 cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc
     1321 atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt
     1381 tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa
     1441 agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc
     1501 aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt
     1561 gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa
     1621 actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta
     1681 atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct
     1741 ttctgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg
     1801 atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg
     1861 cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata atcgacttct
     1921 ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt
     1981 acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc
     2041 actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac
     2101 cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc
     2161 aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc
     2221 tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat
     2281 aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc
     2341 cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc
     2401 aaagaaaagt tttattcctc tttcattatt accaaagtct cttattttaa attgaataca
     2461 ttataatgat actgtaaata tatctttatt attagtgaat ggatatttac ttttaataga
     2521 taaaatatta taactattaa aaataactat tttcattata attacaca
//
LOCUS       DAYYKN010000224         2538 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000224 DAYYKN010000000
VERSION     DAYYKN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.231"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..2469
                     /locus_tag="ACHBB7_002851"
     CDS             28..2469
                     /locus_tag="ACHBB7_002851"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM9210731.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          840 a          455 c          603 g          640 t
ORIGIN      
        1 ggtatatttt tgtaggaggt ggcttacttg aaagaagtcg ttatgataga tgctgcaaga
       61 acgccgatag gaaagtatcg cggaagttta agtccattta ctgcagttga attaggcaca
      121 cttgtgacta aaggattatt agataaaaca aaactgaaaa aagataaaat cgatcaagta
      181 atatttggaa atgtcctgca agctggtaat ggtcaaaatg ttgccagaca gattgctttg
      241 aacagcggtc tccctgtaga tgtcccagca atgacgatca atgaagtttg cggatctggt
      301 atgaaagcag tgatcttagc acgccagctg atccaattag gtgaagcaga acttgtgata
      361 gccggcggaa cagagagtat gtctcaagca cctatgctga aacagtatca gtcagaaaca
      421 aatgaatatg gtgaaccaat ttccagtatg gtcaacgacg gattgactga cgcattttca
      481 aatgcacata tgggattaac cgcagagaat gttgcaacac aattttctgt gagcagagaa
      541 gagcaggatc gctatgcctt gtcgtcccag ttgaaagcag cacatgctgt cgaagccggt
      601 gtattttctg aggagatcat cccagtcaag atttctgatg aagacgtgtt atctgaggat
      661 gaagcagttc gtggaaatag tacattggaa aaactgggca cgttacgtac agtattctca
      721 gaagaaggaa ctgtaacagc aggaaatgct tccccgttga atgacggtgc ctctgtggtg
      781 atccttgcat ccaaagaata cgcagaaaat aataatctgc cttatttagc aaccatcaaa
      841 gaagtagcgg aggtcggtat tgacccaagt atcatgggaa tcgctccaat caaagccatt
      901 caaaaattga cagatagatc aggaatgaat ttatcaacaa tcgatctttt tgagatcaat
      961 gaagcatttg cagcttcttc tatcgtagtt agccaagaac tgcagttaga tgaagaaaag
     1021 gtcaatatat atggaggagc aatcgcatta ggccatccaa ttggtgcaag cggtgcacgt
     1081 atcttgacta cattagctta cggattgctt cgtgaacaga agagatacgg tattgcttca
     1141 ttatgtattg gtggaggttt aggcttggca gtcttgttag aagcgaatat ggagcaaaca
     1201 cataaggatg tgcaaaaaaa aaagttttat cagcttacgc catctgaacg gcgcagtcaa
     1261 ttaatagaaa aaaatgtatt gacacaagaa actgcgttga tttttcagga acaaacactt
     1321 tctgaagagc tctccgatca catgatcgaa aaccaagtga gtgaagtgga aatcccaatg
     1381 gggatcgcgc agaattttca aatcaatggc aagaaaaaat ggattccgat ggcaacagag
     1441 gaaccatctg tcattgcagc tgcaagtaat ggtgcaaaaa tctgtggaaa tatctgcgca
     1501 gaaacacctc agcggctgat gcgagggcaa atcgttctat caggaaaatc ggagtatcaa
     1561 gcagtgatca atgcagttaa tcatcgaaaa gaagagctga ttctttgtgc aaatgaaagt
     1621 tatccttcaa tcgtgaaacg cgggggcggt gttcaagata tctctactcg ggaattcatg
     1681 gggagtttcc atgcatactt atctattgat tttttagtgg atgtaaaaga tgcaatgggc
     1741 gctaacatga tcaactcgat tcttgagagt gtggcaaaca agttacggga gtggttccct
     1801 gaagaagaga ttctattcag tattttaagt aattttgcta cagaatcatt ggcttctgcc
     1861 tgttgtgaaa tcccattcga acgcttaggg agaaacaaag aaatcggtga acaaattgcc
     1921 aaaaaaatcc aacaggcagg ggaatatgcg aagcttgacc cttaccgtgc agcaacgcac
     1981 aacaaaggga tcatgaatgg tatagaagct gtagtggcag ctacaggaaa tgatacacgt
     2041 gcggttagcg cttcgatcca cgcctacgcg gcaagaaatg gattatacca aggattaaca
     2101 gactggcaga tcaaagggga taaactagtt ggaaaactaa cggtgccttt ggctgttgcc
     2161 acagtaggcg gagcttccaa tatcctgcca aaagcaaaag catctttagc tatgttagat
     2221 attgattcag ccaaggaact agcacaagta attgctgcag tagggttagc tcaaaacctg
     2281 gctgctttgc gtgcattagt tacagaagga atccaaaaag gccacatggg acttcaagct
     2341 cgttcattag cgatctcaat tggggctatt ggtgaggaaa tcgagcaagt tgcgaaaaaa
     2401 ttacgtgaag cagaaaagat gaaccagcag acagctatac aaatattgga aaaaatccgg
     2461 gaaaaataaa aaagcaatat ctttagaaga ataaagcaag atttacagtt attttattta
     2521 ctgcagatct tgcttttt
//
LOCUS       DAYYKN010000225         2522 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000225 DAYYKN010000000
VERSION     DAYYKN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.232"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..462
                     /locus_tag="ACHBB7_002852"
     CDS             <1..462
                     /locus_tag="ACHBB7_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM9210732.1"
                     /translation="YKFIVAIGDCVVRKKIIKKLNMPSEQYGIAVHPSAIVAKEISIG
                     YGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQ
                     IGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            413..1594
                     /locus_tag="ACHBB7_002853"
     CDS             413..1594
                     /locus_tag="ACHBB7_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM9210733.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            1607..>2522
                     /locus_tag="ACHBB7_002854"
     CDS             1607..>2522
                     /locus_tag="ACHBB7_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HFM9210734.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEP"
BASE COUNT          897 a          326 c          500 g          799 t
ORIGIN      
        1 tataaattta ttgtagctat tggtgactgt gtagtaagaa aaaagattat aaaaaaattg
       61 aatatgccaa gtgagcaata tggaatagct gttcatccaa gtgctatagt ggcaaaagaa
      121 atttcaattg gttatggaac tgttattttt gcgaatgctg taattaaccc agatgctgta
      181 attggagagc atgctataat aaacacaggt agcattgttg agcatgattg tagaattaat
      241 aactatgttc atttatctcc aggtgtatgc ttagcgggtg gagtacatgt aggtgtagga
      301 actcaaatag gaattggttc tcaatgtatc cagatgaaga aaattggatc ttggtgtatg
      361 ataggcgcag ggagcactat tgttaaggat attccctcac attccttagc ttatggcaat
      421 ccagctaaaa ttaagaaaga aggaattaat tttgaattct agaattttat tatcaagtcc
      481 tcatatggga ggaacagaag aaaaatatgt tgctaaggct tttaaagata attggatagc
      541 gccattaggt gaaaatgtgg ataaatttga agaaagtatt acaaaatata ctggttctaa
      601 gggcggagct tctgcgttaa gttcaggtac agctgccata catcttgcct taagactgct
      661 taatgttgga aaaaacgata ttgtctttgc tccttcattt acttttatag ctacagtaaa
      721 tcctatttta tatcaagggg ctgtaccagt gttcattgac tcagaaccta atacatggaa
      781 tatgagccct gttgcactag agagcgcatt agaagaagct gaaagaaaca accaattacc
      841 taaggcaatc atcgtagtga atttatatgg tcaaatggct gattatgaac gaattttagc
      901 tattgcaaac caatttaatg ttccagtgat agaagatgca gcggaatcgt taggttctac
      961 ttataaggga atttcgtcag gcttatttgg gacaattggg atctattcat ttaacggtaa
     1021 taagatcatc acaacctcag gtggaggagc tctagtgtct agtgataaaa aatatgttga
     1081 gcattcaaga tttttagcaa cacaagcaag agatcaagca ccgtattatc aacatagcga
     1141 aataggttat aattaccgtc taagcaatat ttctgccggt atcggacgtg gacaaatgga
     1201 agtattgaat gatagagtag ctaaacgaag agaaattttc aatcgatata aggaagcttt
     1261 ttctggaatt gatagaattg aaatgatgcc agaagtggat ggcatggtgc caaatcgttg
     1321 gttgtctact ttaacgatca atgattataa ttctattgat caagtagtta acgtaataaa
     1381 taaactaaat gaaaaaaaca ttgaaacaag agctttgtgg aaaccactac atttgcaacc
     1441 agtatttcaa ggggtaaaaa tgtattacca tgataataat cttagtgtgt cagaaaagtt
     1501 atttaatagc ggcttatgct taccttctgg aagtaatatg acagatagtg aacaagaatt
     1561 tgttattgat gagtttaaaa aaatattcaa gtaggtgtga tattttatga acataggaat
     1621 tattggttta ggttccatgg ggaaaagaag acttagactc attcaagaac actttaatga
     1681 tattttacta tgtggtgttg atactaatga ggcgagacga aaggaagttg aaaaggaatt
     1741 tggaatacca acttataata gtctaacaga ggcaaaaaat ttgtttgaaa taaattcagt
     1801 ttttgtttgt acatccccta taacccatga atttattata aaagaggctt taaatttaga
     1861 ttgtaatatc tttacggaaa tcaatttatt aaatgaatat tatgatgaaa taatcgaagt
     1921 agctaaaaag aaagatagat tactttatct atcttctaca tttttgaaga gaaaagaaat
     1981 tcaatatatt aagtctaaag tgacgcataa taaaaaaatt tcttatcaat accatgtagg
     2041 acagtattta cccgactggc atccttggga agattatcgc aatttttttg tttcaaacaa
     2101 aaaaactaat gggtgtagag aaatattcgc tattgaatta ccttggatta tagatacatt
     2161 tgggaaagtt aaatcgttta ctgtaatgac ccagaaatta tccaatcttg atattgatta
     2221 ttcggacagt ttcactctta ttattgagca tgaaactggt atagttggaa ctttaagtgt
     2281 agatgttgtt tcaagagtag ctaaaagaaa tttaactata attggtgaaa agattttaat
     2341 agaatgggaa ggtaacccgg attctctatt ttcatggaat gaagaaactt ctagtatgac
     2401 agatattcaa ttgtacgatg aggttatgca tgataataat tattcacata ctattattga
     2461 agatgcatat ttagaggaga ttaaagaatt tttcacgctt ttggaaaaga aaatggaacc
     2521 cc
//
LOCUS       DAYYKN010000226         2495 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000226 DAYYKN010000000
VERSION     DAYYKN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.233"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACHBB7_002855"
     CDS             <1..963
                     /locus_tag="ACHBB7_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9210735.1"
                     /translation="PINTFAYTINNEFNLGPNEGSSQVANNQYILLHETANETATGRN
                     EAQYMKRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIYRPDVNQAYNTLGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
     gene            1267..1674
                     /locus_tag="ACHBB7_002856"
     CDS             1267..1674
                     /locus_tag="ACHBB7_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210736.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            1688..2089
                     /locus_tag="ACHBB7_002857"
     CDS             1688..2089
                     /locus_tag="ACHBB7_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210737.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            2091..2462
                     /locus_tag="ACHBB7_002858"
     CDS             2091..2462
                     /locus_tag="ACHBB7_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210738.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          913 a          373 c          463 g          746 t
ORIGIN      
        1 cccattaata cgtttgctta tacgatcaac aatgaattta atttgggccc aaacgaaggt
       61 agctctcaag tagcaaataa tcagtacatt ttactgcatg agacagctaa cgaaacagca
      121 acagggcgca atgaagcgca gtatatgaaa cgttcatgga ctagtgctta cactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc taacagtccc gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tagctagaga ttcagcaatg
      361 aaatatggta ttccacttac actagacaca ccgtataatc aaccagggat caaatcgcat
      421 ttatgggtaa cacaaaatat ttggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcctat gatttggctc atggatttac cgatgaaaat
      541 ccgacaactt cagatgataa accagtcatt gatccaactc gagcaggtgc tgcaaatcct
      601 accctgacag atggaacaaa ttacgcccac attgatcagt tcggagaaat cgaaaacgca
      661 aacttacatg tggctggttg gcacatcgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaaaagctg atggaatata tagaccagat
      781 gtaaatcaag cttataatac tttaggaaat gttggctatc atgtatcttt taacatgcgc
      841 aacttcccta ataagaaagt ctatgtaatg atgcgggcaa cgaatgatcc agaagggaac
      901 actaaaggcg gagcacaaga ttttcatgat aaacgctggt atttaaatat tccgcaacga
      961 taaaaatagc ccctcatttt tgaggggaag tacatatagt tgttgatata gttgctaata
     1021 ttgttagcta atttgttagt aaaactgtat acgaatttag atagtaaaaa aacagtttgt
     1081 tccttatata gaggcacttt tactactgat taaatgtcag atatgcttac aaatatactt
     1141 ataaatatgc ttacaaaatt agctaatatt tccgttgaca aagctttgtt actattgtta
     1201 aactaaattg actagcaaaa gtgaaacata aatttattaa ataaatgtaa aaattaggag
     1261 aaatcaatgt cttatagagt acaattaata atttcagaag atgtagaaag tcagcaattt
     1321 ggcactaatg ttattaataa agtaattaat ccagctcttt ctattaatgc gcctttaatt
     1381 cctactgctt tatcattttc tgtaacagct gtggtgtctg agatagagga tacagaaaag
     1441 ataaaaatag tagaaattga agttttaaac aagaatgaaa aacaaatttt ttctacgggt
     1501 gaagtatcgg taagcttgcc tcctcaagtt aacgatataa actttaatat aaacgccaga
     1561 aatgtcttgg ttgaagaagc aggagagcat tatgctgttg ttaaatttaa tggaactgag
     1621 attggaaggc aaatctttga tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac
     1681 gatacggatg cttgatatag taccatcaaa tacacatcgg agtggtagct taataaattt
     1741 tgctgcgatt tttgcttgcg catcggtaat tctagcatct ccagttgttg tggatgcaag
     1801 ttctactccg aaaactaaaa atgataatca acttgtaatt actacaaaaa atgagattaa
     1861 cacaatttca agtaatgatg gcaaaatttt tgatgtgata aatacggttt taaaaagaaa
     1921 agctgaaatc aatataaaat atgatgaaga tttaaacttg tatttctttg ttatcaaaac
     1981 aacatctgaa ttgttttcct cagattacga tgttttggat actctcgata atgtattatc
     2041 tgactataaa tatatgggga aatctgtagt agcaactttg ggggaataaa atgtttgact
     2101 ttaatgaata tttatctatt tccaaacaaa ttgaatcaga tgataaatat aattctaaag
     2161 aatcttatag aagaactgct gtttcaagag catactacag tgcttttaaa aaatctgatg
     2221 agtatttgaa agaaaattat gatattattt ataatggaag tagcggaaaa ggaagccacc
     2281 aaaccgtttg gaatttgttt tcaacagtta aggaattgaa taccttagga atacataata
     2341 gtgggtatag aatgttagag aaaagaaaat gtgctgacta tattcctaat gaaaccatta
     2401 cgaaaacaga tatggcgctg atgaatcgag aagcagaaaa aataataaac aaactcacct
     2461 aatgaatttc aagcgagttt gtttgttatt ttttt
//
LOCUS       DAYYKN010000227         2446 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000227 DAYYKN010000000
VERSION     DAYYKN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.234"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..139
                     /locus_tag="ACHBB7_002859"
     CDS             <1..139
                     /locus_tag="ACHBB7_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9210739.1"
                     /translation="RLVLENELTYTEIADILGFHSIHHFSSAFKKYTGLSPSKYYLSL
                     K"
     gene            280..1893
                     /locus_tag="ACHBB7_002860"
     CDS             280..1893
                     /locus_tag="ACHBB7_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9210740.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          773 a          433 c          464 g          776 t
ORIGIN      
        1 acgtctcgtg ctagaaaatg aattaaccta tacagaaatt gcagatatat taggatttca
       61 ttcgatccat catttttctt cagcttttaa aaaatataca gggttatccc cttcaaagta
      121 ttatctatcc ttaaaataga aaaaaatttt taggttttat ccacaaatta tttctctata
      181 ttttctgtct attcaaaata aacaataatt ttccggcaca attatttgct gttttttacc
      241 ataacatagt acgatcaacg tagaaaagga gatgttttta tgaaagttgt tgtcattggc
      301 ggtgtagctg gcggtccttc atttgccact cgtttccgtc gattgaatga agcgcacgaa
      361 atcattatct atgaacgcgg agaaaatatt tcttatgcaa gttgtgcttt gccttattat
      421 ttaggaggtg tgatcactga ccgtgactcg ctgatcgaac gtacaccaga aatattgaaa
      481 acaaaaaaca acatcgatgt ctttactaaa cacgaagtaa cagcaatcga tccttctact
      541 aagcgattaa cagttaaaga cctatccaca aatgaagaaa taaaaacaga ttacgataag
      601 ttgatcatct cttctggtgc tagaccagat tatccggata ttcccggagt ttttgaagca
      661 gaaaacggct ttgtcctccg tagtgtgacg gatgcggatc gaatcaaatc gttccttgaa
      721 gaaaaaaatc cacaacatgt cgtcattctt ggtgcaggtg ttatgggtct ggaattagct
      781 gagaatttca agcatcgcgg cttagacgtg actttaatcg atcaattgcc acaagtcgct
      841 ttcccttatg atccagaaat tgctaattta gtttatgaca aattgctgaa agaaggatta
      901 gccgttcatt tagaaacaag agttactgag atccgtgata aaggtcgaga aatagtatta
      961 tcagatggct ccgtcctttc tgctgatatg ctaatttttg ctgttggtgt ttctccgaag
     1021 aatgaagtgg tgaaagcaac cggcatacaa ttatcagata caggtcagat cattgttgat
     1081 gaccagttac aaaccaatct gccggacatc tatgcgattg gcgatattat cgaaacgact
     1141 agtgtagtga ctggtcagcc gatccaaagt atgctttcca gtgcggccaa tcgtcaagga
     1201 catatgctgg cagatatttt aaatggtacg cctatgcgct atcgcggata tattggtgca
     1261 ggtgtcgcaa aaatctttga tcatacagca agttatgcgg gaatgacaga acatgaatta
     1321 aaagcatcag gcataacaaa ttataaaact gtttttatca ctccctttga ccatgcctat
     1381 ttctatccag aagctacaag attaaatcta aagctgattt ttgatgcaga tagcggtcgt
     1441 attttaggtg gacaagcatt tggagaaaaa ggtgtcgata aacggatggg agaactttct
     1501 gtagcaatca ccggaaactt gacagtcttt gatttgcctg atttggagtt gccttactct
     1561 ccaccgtatt ctactacccg tgacccgttg aatatagctg gttatgtcgc aatcaatcaa
     1621 atgacgaata tcgtagaaac gatcaaagca agtgatatac ccgaaaacga tttgaaagaa
     1681 gcgttctttt tagatatacg tgaacctaat aaagcacctt ctggcagtat ctcggcaaca
     1741 aaaaatattc caatgaatga gttacgagat cgaatcaatg aaatcccaaa agataaaaaa
     1801 gtttacatta ctttcagaag aggattgaat acttatactt ctgcccgaat cttggcaggt
     1861 ttgggtatca aagcgatttt gattgaagaa taaaaaaacg accgcggtgg gtagccgcgg
     1921 tctaggagtt atttttactt gaggagtaaa aatgaaaaag tatatagggt tgttggttgg
     1981 tatgtatata gaatagaaga taaatgtgaa gaaattgtgc attatttctt cacatttatt
     2041 aggttatagt ttaagaaagt ttaaacattg cgcaatttaa ttattttagc tgtttctttt
     2101 cataaaaaaa catgttgtga ctctgagaca aatcatagtc catagtgatg tctctagagt
     2161 aactacgcct aatattacga ggcatcatat taatctatca gcactagtac ctaaactagc
     2221 atccctttcg aatatttttt cttatataat aaagtttgct ttcactcctc taatctcacc
     2281 aaatcgactg gcttgcgttg tgtgatacta ggaactgatt ggtttagaac gacttttcta
     2341 ttgtaatcat atttttcgcc atcctcgttg tcttctaatc cttcaatgaa tgatccctaa
     2401 tgtgtgtatt aaataattta atttttttgt tcctatttat ttaaca
//
LOCUS       DAYYKN010000228         2388 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000228 DAYYKN010000000
VERSION     DAYYKN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.235"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHBB7_002861"
     CDS             207..2069
                     /locus_tag="ACHBB7_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9210741.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2388
                     /locus_tag="ACHBB7_002862"
     CDS             2069..>2388
                     /locus_tag="ACHBB7_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9210742.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETA"
BASE COUNT          857 a          353 c          466 g          712 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagc
//
LOCUS       DAYYKN010000229         2293 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000229 DAYYKN010000000
VERSION     DAYYKN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.236"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACHBB7_002863"
     CDS             complement(51..1598)
                     /locus_tag="ACHBB7_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM9210743.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACHBB7_002864"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACHBB7_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM9210744.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="ACHBB7_002865"
     CDS             complement(2043..2237)
                     /locus_tag="ACHBB7_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210745.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          477 c          408 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DAYYKN010000230         2188 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000230 DAYYKN010000000
VERSION     DAYYKN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.237"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..804
                     /locus_tag="ACHBB7_002866"
     CDS             130..804
                     /locus_tag="ACHBB7_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210746.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQA
                     LPENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQ
                     AAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPC
                     SKCYGN"
     gene            complement(966..2138)
                     /locus_tag="ACHBB7_002867"
     CDS             complement(966..2138)
                     /locus_tag="ACHBB7_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9210747.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT          700 a          435 c          395 g          658 t
ORIGIN      
        1 tgtatcaaaa cgggattaga ttaagtttcc tcaatcacta ttcataacac aattattata
       61 acaagtaagt aaacaattga aaaggacatt tattgaaaac ggttgttttt tacttaagga
      121 ggaaatttag tgaaaaaatt agttgcattg tttttggtcg caggaagttt gttgagtggg
      181 tgtggtgcta ctacgtctac tactcaaagc acaagttcag ctcaggcttc cagcagcagt
      241 accacgatct ccgaaagtac ggcagattca tcgaaagaac aagaacaaat tgaaaagaaa
      301 aaagaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaaagt gacagaagcg
      361 gaatctgctt tgtcacaagc tgaagcaaat cctacaaaag aaaactatgc aactgcttca
      421 gcagctatcc aagcgctacc tgaaaacaaa caagagttga caagccgtct tgctgcaatt
      481 gattccacaa tcaaagcaaa agaagcagaa gaagaacgga aaaagcaaga agaaattgca
      541 cgacaacaag cagaacaagc gcaacaagaa gcaatcgcac agcaacaagc agaacaggcg
      601 cgacagcaag cagcagctga acaggcctct cagcaggcac aagccgcagc tcctgagcaa
      661 aataatgaac aaactgtcta tgtcacaccg accggttcaa aataccatac acataaatgt
      721 ggaaatggta cgtatacccc tgccacttta agcgaagctc aagctcgtgg tttaacccct
      781 tgctccaaat gttatggaaa ctaattcaaa agttatacta taataataaa attcttctga
      841 aacataaaaa aatagaagct ggcgttccag atagtaaacc aaaaatagca gtttacacgt
      901 ccatttcgtc agcttctctc ttttttcatt tagtctgttc cctctgcttt tttggacatc
      961 ttcattcatt ctttctcaat caaatagtac acctcttttt cttttcgcag tttatatctt
     1021 ccttttttca atttcattga aaactgtttc gaatgtttat gttttacatg gattttaagc
     1081 aggagctcct catgttgcaa gcgatagttc ctgcctttta aatcagaaaa tctcactagt
     1141 ccgtattcat cagaagcttt cacaaatgga actttttctc tagtaatata gtgtttgccg
     1201 ttgagcgttt ttacagaaaa gaccattcct tcgattggtc gtccgtttgt gttctttaca
     1261 taaaaagata aaggatagtt tcgcttagcg atggccaaag aatctaaata agcgatgagt
     1321 agccaaacta agccgattga tccaagtagt ataccgataa accaaataaa aaatttccat
     1381 tttttccatt gatcaattcc ttggatcgct ttttttgcct cttttgcttg ataaggaata
     1441 cgatgccccg tcacaagcag tcgatgtgta ttgaccatat aaggagtaca ggtaagcaaa
     1501 gtcacgagat ctctgccttt ttggataccc aaagaatcta cttcatcagg taagaccact
     1561 tggatttttt ctacttgata cgccaatgtc tttccattga tctggatgaa aaactggtct
     1621 ccttttttca acttaggcaa atctgtaaat aattttgctt ctgggagtcc acgatgccca
     1681 gaaagaacgg catgggtgtt tttcccgcca gtcggataag aactttcttc caataatgac
     1741 gttccttttt gcaaaaatat ttccgttgtc tgatcgaata taggtaagct gacattgatt
     1801 tttgggattt cgatgactcc aagcatatgt tccataaaaa aggcattact aggtaaatct
     1861 tttaatgctt ttgcatcaac tgcttggttg aaactgccta tccctggtga tacgttttgc
     1921 tctgccaact gttggttttt ctttgtcatt ttttcctgct gtttttttat ttcctctgaa
     1981 ttcttattcg atgcttgctt ttgataatgg atgatcattt gctgggcaag aaaatcattc
     2041 agtgaatctt ctacaaaagg ataagaaaag atcccgatcc caacgactaa aatgattgcc
     2101 atgaagatgt ccagtattct tattctgctt ttattcatca tgtccaccta ttttctaata
     2161 tgtatatgaa gaggttgtga aatcagcg
//
LOCUS       DAYYKN010000231         2182 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000231 DAYYKN010000000
VERSION     DAYYKN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.238"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..181
                     /locus_tag="ACHBB7_002868"
     CDS             <1..181
                     /locus_tag="ACHBB7_002868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210748.1"
                     /translation="DSFWNHDQGRTYTKIFCPFDPNRLTTNLVSNIDWYVVKPANGKV
                     VYSTPRALKTASSGV"
     gene            complement(136..2169)
                     /gene="brxL"
                     /locus_tag="ACHBB7_002869"
     CDS             complement(136..2169)
                     /gene="brxL"
                     /locus_tag="ACHBB7_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFM9210749.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
BASE COUNT          696 a          483 c          294 g          709 t
ORIGIN      
        1 tgattctttt tggaatcatg atcaaggacg gacatacaca aaaatatttt gcccattcga
       61 tccgaataga ttaacgacta atctagtaag taatatagat tggtacgtag tcaaaccagc
      121 aaatggaaaa gttgtctatt ccaccccaag ggctttaaag acagcatctt ccggcgtttg
      181 ataaaaaatc aaattaaagg tacccattaa ttctgggggt acagttccaa tatcagcagc
      241 gcttatcata ggtacaagta cttttttcgc tccggcatct tttgctactt gtaaacagtt
      301 agctaaatta tctaccttaa tcatagaacc accaattgta atatccccta aaataactaa
      361 acttggtaat acaggtttat ttaaggaaac tgagcataaa gctattatag ttgataacga
      421 taatgttttt gtcataccaa tgccattcat atcttgaaca tgaacgacaa aatcttttgt
      481 cgtcaaacta atagactgac taacacttcc ataattagct cttaaatatc ggtatgccga
      541 atccatcgct tctcttgctc cagcatctga attgatccca gtacgttcaa actttccatt
      601 tcccgaagtc atttgcgttt ccaatttata ggtacctaat tgcccagttt tgccataaga
      661 taccgtgtac aaatgccctg gcttccctaa cccttctgga atcaaagttc ctccaccttg
      721 ttcaggaaca gaaacatact cttctcgctc ttcttctaaa tcaatgtatg aaaaattgac
      781 atcataaaat tccattccac caattttctt taattgttct tttacacgac gacgcatttc
      841 aagagcaaat cttagtattt cttcgacatc acttttttca aatctaccat caggatacaa
      901 cagtttcatc atcccagata caatccgacg aactgcaatc gtatcccgct gatttaattg
      961 agaaccaagt ttgaaatatt tatcaaaact atcgccaaaa ctaacttttc gaagctcacg
     1021 catgacttca gaaaaataat ctgtaatgaa gccatagtca tttgtaaaaa aatctggtcg
     1081 atatttagga atttcccaac ctggtaaata acaatgcatc cgatccaaaa aggctgaatc
     1141 cgtccccata acctccggaa atggctcaaa taaattcgaa gttttcaata ttacatcaac
     1201 gctttgatta atatttccta caaaagccat tgatgctgaa gcattctttt cttctttgcc
     1261 tctagcaaaa gatcctgaag ccatgtaatc tttcatgatt tgaacgccat cattatcctt
     1321 aaatttaatc ccagcaactt cgtcaaaagc aacacagtcc cacataccta caagtcccat
     1381 tgtacgacta gccatattat agaaaaggtt tgctactgtt gtttgtccac ctgaaacaag
     1441 gattgagttt ggtgaaattt ctttatatac atgcgattta cctgtaccac gtggtcctag
     1501 ttcacagaga ttaaaattat tttcaattaa tggaataagt cttgctaaat gaagccattt
     1561 taccctttta ttaaattgtg taggttccat accaatggac cgtagaagta agtcaatcca
     1621 ttcatttgta gtaaactcct tgcgagccat cttgtaagcc tcaatgtcta aaccaggcat
     1681 ttggattgga tcaacctttt ttgcaataaa agggctattc caacgatcat tctcatcata
     1741 ccaatattcc atcgaaacaa tacaccagat ccccccacct aataatcgat caaatttttt
     1801 cggatactct tcactaattt gaacattttt taatcccagg ttagagaatt ctgcttcata
     1861 acaatctttc ttttcattta aatgtacagt tactttatca ataactgtat aaccacccaa
     1921 ttcacgaatt tttgctttta ctttttctgc ttcatcagga cgcacatagt ttcgagcaag
     1981 aatattttta acattttcca cacctttttc aaccaatgtt tcatccaatg acgaagcata
     2041 cataccaagt aaatattcca atacataaac aggtacattc gcaccttctt taattttctt
     2101 tgttaaatct tttctgacaa tctttccggg aaagaattca gataacttta aaatttcctg
     2161 ttgttccact agaattcctc ca
//
LOCUS       DAYYKN010000232         2104 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000232 DAYYKN010000000
VERSION     DAYYKN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.239"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..993)
                     /gene="cysK"
                     /locus_tag="ACHBB7_002870"
     CDS             complement(67..993)
                     /gene="cysK"
                     /locus_tag="ACHBB7_002870"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM9210750.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1133..2020)
                     /locus_tag="ACHBB7_002871"
     CDS             complement(1133..2020)
                     /locus_tag="ACHBB7_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9210751.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          579 a          471 c          356 g          698 t
ORIGIN      
        1 ttttgtgtgt tcttttcatc tttaaaagtt ttttacataa acctatctgg agaaaaacca
       61 agtgccttat atctcttggt aaagggctgt agataaataa cgttcaccat tgtcgggaac
      121 cacagctaat accttcttgc cttttcctaa ttcttttgcg acttttagcg cggcagcaat
      181 tgcagcgcca gatgagatgc ctactaaaat cccttctttt cgcccaactt ctctagctgt
      241 ttccatcgct tcgtctccac taatagaaag gactttatca tacacagaag tatccagtgt
      301 tttaggaaca aaacctgtac cgattccttg gattttgtga ggtcccgctt ctttcccttc
      361 taaaatagca gattctgctg gttctacccc gattaattcg attttcggat acacacgttt
      421 taattctcgg ccagcgccag tgatcgttcc tcctgtaccg atacctgcaa caaacgcgtc
      481 caagccattt actccaaatg cttgatgtat ctcaggacca gtcgtcttct catgcacaag
      541 aggatttgct tcattttcaa attgtaatgg taaaaagtag ccgttttctt ttgccaaacg
      601 ttctgcttct ctgattgatc cagaaatacc atccgcacca ggagtcaaaa tcaactctgc
      661 gccgtaccct ttcatcaata aacgtctttc gatactcatc gtctcaggca tcacgatgat
      721 tactttatat ccttttgcaa ctcctaccat cgacaatccg attcctgtat ttccagaagt
      781 aggttcaatg atagtatcac ctggtttcag tagaccatcg tgttctgctt tttctatcat
      841 acttaaagca atacgatctt ttacacttcc tcctggatta aaaaactcaa gctttacaaa
      901 aacatctgct gaatcttctg gtacgatttt atttaatttg acgattggcg ttttgccaat
      961 caattcggtg actgagttat agatagctgt catcttcaat tcctccttgt ctttgctctt
     1021 cttacttatc ataatacaat ctgttcattt atggcgagtg attcgtcttt gactagcaaa
     1081 aaaaagacaa agatatacca tatatggtaa tttctttgcc tttttatcta aatcaaacgt
     1141 ttagttcttg accaactaat aaagaataac tggacataga catgccattt ttcgccatta
     1201 gatcatcaac agacatgcca aatttttgtg caatatccca tagcgtatca cctgacacga
     1261 ctgtatatgt ctgaccatta tttgtattta catctgttgt tgaagatgat gacgttgaag
     1321 tcgtcgtagt agtcgttgtt gttgtacttg tggttgttgt agccgaacct gttgatggcg
     1381 tatcaaaacg ggttaaatta taagtattaa tcaatgagtt caatttggcc ccataatttg
     1441 gatcggtagc ataacgacct gtcaagtacg ctgttgcatc ataaaaactt gttgtattgc
     1501 ttttccacac gccagcataa tgataatcgc ccgtggaaaa actggttgaa cgaagaacat
     1561 tcgcatggtc ttggaaggat tcccaataag aagaatattt tcggaatggc tcattcatcg
     1621 ttacccattg tccgtttaca tattctttgg ttgccatata aacgacctgt ccgttatagc
     1681 ttcctttcac accgaataga ttataatttg gcgcactagc taatccggaa gtgccatatg
     1741 cactttccaa caacgcttga gcgatcatga ctgaagcata caagtcatta cttgcagcaa
     1801 cttctgacgc agaccagccg atttgatcga taaacgcttg aggatcagcg actgtttgag
     1861 atccttgttc atctgcatga acatgttgcg gtaccgctgt aaccatcatc ggcccaacta
     1921 aaagactcgt tccaattaaa ggagcaatcg cttttttatt gatcaccatt tttcttttag
     1981 gtctatggtg ccgggcactg cgggtcttca gctcttgcat tcaaattcct cctcaaaata
     2041 aaaaaactta ataaaaagtt ctcttcttaa aacaaactta attaattaaa ttatactttt
     2101 aatt
//
LOCUS       DAYYKN010000233         2064 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000233 DAYYKN010000000
VERSION     DAYYKN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.240"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1364)
                     /locus_tag="ACHBB7_002872"
     CDS             complement(45..1364)
                     /locus_tag="ACHBB7_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9210752.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="ACHBB7_002873"
     CDS             complement(1361..2014)
                     /locus_tag="ACHBB7_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9210753.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          688 a          312 c          161 g          903 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atgg
//
LOCUS       DAYYKN010000234         2057 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000234 DAYYKN010000000
VERSION     DAYYKN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.241"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            494..715
                     /locus_tag="ACHBB7_002874"
     CDS             494..715
                     /locus_tag="ACHBB7_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210754.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            716..973
                     /locus_tag="ACHBB7_002875"
     CDS             716..973
                     /locus_tag="ACHBB7_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210755.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1109..1711)
                     /locus_tag="ACHBB7_002876"
     CDS             complement(1109..1711)
                     /locus_tag="ACHBB7_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM9210756.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1778..1933)
                     /locus_tag="ACHBB7_002877"
     CDS             complement(1778..1933)
                     /locus_tag="ACHBB7_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFM9210757.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          603 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt tttctactag tcatttcgat aatataaaat tgaccacaac acagaaaact
      421 tatattataa gcttatgtcg taagtccgac gtactcacgt aatatagacg gacgttaaat
      481 ggaaggagca taaatgagag tgataaaatc agaaaaattc actttggccg aaattatgac
      541 gaccagcgaa cttgcacaag aacttgatta caaccaagct tacgttttgc gcttagcgaa
      601 agaacatctg actgaaggaa aagaatatcg ctctgccggt agaagaaact atcttttcag
      661 cccagaagcc cttaaaaaac tagaaaaagt cataaccacc aaagaaggtg agtaaatgcc
      721 taaagaaaca actatcaacg ttgaacaact agaaaaattg ctagaatcat attccaatga
      781 ttttatcgaa ttgaccggcg gaaaagtcga agaaaccacc gacctctttg acctacttct
      841 cacaaatatt ctggaattaa actctgaaga acaaaaagag cataagggaa aactgatagc
      901 accttcctac aggaaggtgg ctaagtcggc tgtgccaact ggttttctta caaaacaacc
      961 ttatattttt tgatatcaca aaaaaatcca ttttggacct attttcggtc ataatatagt
     1021 acctactttt ggtcatagtt tcgtccgtag ttttggtcat agtgactaag actttcacgc
     1081 acaaaaaaag agctgaaatt aacagctctt atcctttatt tatttttgaa tttacacttc
     1141 caccatgacg attcgatttt atatattgcc cgataatata agctgaatta tcacaaagaa
     1201 gcggaaacca atcatcaaat ctttctgaca ttgcatagtc aattaaatct ttgatttcag
     1261 tgatattctt agaatcaaca aacgacatca tctctctaat caactcatat cgttctgcac
     1321 tcgttactga caaataacta gcaatttctg caccaccatg agcgataata tcacttttag
     1381 catattgaaa tttttctgga ttatccatat gagcaaaata tcgcaccata ccttttgcat
     1441 tagcacattt ttgcgggttt ggagaccgaa gcttttgagt tatctcacgt atttgatcat
     1501 acgtcttttt accgctaaac attatcaaaa catgccaatg aggctttttc aattcaccgt
     1561 caggattcac atctttatca tgaagtggac tctcaaccca aggaacatgt aactcatcta
     1621 aaaactccct ccaattttca ggagccgatt caggatagac tacaaacgtc caattccttg
     1681 tgcgttcatc tttagttttt ctactcgtca tgctataatt accttaattg aacaacagtc
     1741 taacagcaaa agcagtgcgc caacactaat gcttttgcta ttttttttgc tcattttcct
     1801 ttccgttagc attaacccaa attgtaataa gaccagactt actaagaccc atttgtttcg
     1861 caattttatc aagattatct gccacttcct tagtaagcgt tacaacgact ctcttttttt
     1921 cattactgat cataattcgt atcctccaac gactttttct taaaaaccat agtaacaaaa
     1981 aagtaactaa ggtgcaacag tttagatact aattagcgaa taataggcct taaacccttg
     2041 gcattactga ttttgtc
//
LOCUS       DAYYKN010000235         2055 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000235 DAYYKN010000000
VERSION     DAYYKN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.242"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..263
                     /locus_tag="ACHBB7_002878"
     CDS             <1..263
                     /locus_tag="ACHBB7_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM9210758.1"
                     /translation="IEIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEM
                     PESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            264..1016
                     /locus_tag="ACHBB7_002879"
     CDS             264..1016
                     /locus_tag="ACHBB7_002879"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM9210759.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1021..1968
                     /locus_tag="ACHBB7_002880"
     CDS             1021..1968
                     /locus_tag="ACHBB7_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM9210760.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          721 a          271 c          403 g          660 t
ORIGIN      
        1 ttatagaaat tcatttagat tattttggaa gagttcctac aagaaaattt caattgttta
       61 ctttagatga atgtatcaat gttgacttat taaataataa aataagtttt gaagaaaaca
      121 ttgttaaaga aatgcctgaa tctgctaatg atatgtatga gaaggagttg aaatatttct
      181 ttgaaaacgt agtaacaggc aaagaaaact ggaatgattt atatcatgca aacgaagtat
      241 tgaaaatagc tgaggagaaa taaatggaaa ttttatttac tatttgtggt agagctgggt
      301 ctaaggggtt taaaaataaa aatttgaaat atttggcagg aaagccccta gtccatttcg
      361 ctttagcaat gatagatatc tatcaacaaa aacatcctga acataattgc acgacttgtt
      421 tgaatacgga cagtaatgag ttagtagaat tgacgactaa gttaaatccg tctgtgaaaa
      481 ttgtagaacg taaacctgaa ttagctacag atactgtagg gaaaattgaa gtgatacgtg
      541 attcattctt acaactagaa agagaagata atatatttga tttaattata gatttagatt
      601 tgacttcacc tttacgtgaa gtttctaatc ttgaagaatt aatagatgaa catctaaaaa
      661 atttagataa agacgtctac ttttcagttg tttctgcaag acgtaatccc tattttaata
      721 tggtaaaaag aattgaaaat gaagttacta tagttaataa atcaagatac acatctaggc
      781 aacagacacc tgaagttttt gaaatgaatg cctcaatgta ttcttttaaa cctgaatttc
      841 taaaaaaaag taattatatt tttgatgggc aatgtgggat aataaaaatg agagattatc
      901 taattttaga tattgatagt gaagaagatt ttaaatggtt agaatttttg tttcctaaat
      961 ttttagaaga ttcttcggag cttcaagata tctataatta tttatatcgg aggtaaaagg
     1021 atgagctgtt atcttatcac cggtggggct ggcttcattg gttctacctt agccaatcat
     1081 ttaggagaag aacatgtggt aatcgtcgtc gatgacttat caatgggaaa agtagaaaat
     1141 cttgatatga cgaaaaatat cacttttatt gagggagatg tcgctgagaa atccttgatg
     1201 gaagagatca tgaaagctaa taaatttgat tatatctttc atttagcagc agttgctagt
     1261 gtggcagatt cagttgcccg accagtagaa acacatcgtg tgaattttga aagtgtatta
     1321 atgattttag aattgatccg caagtatcaa cctgatttaa aacggatcgt gtttagttct
     1381 tcagcggctg tttatgggga tgaaccgact ttaccaaaaa aagaagagtc cgtgattcgc
     1441 ccattgacgc cttatgcggt tgataaattc gctgcagaac aatatgtgct agattattgt
     1501 catttatatg atgtacccgg tagcgcagtt cgttttttca atgtctatgg tccaaatcaa
     1561 aatccgaatt ctccatacag tggtgtgatt tctattctcg tagatcgcta taagaaacaa
     1621 atcgtaggag aagcaacttc atttacatta tttggtgatg gaagccagtc tagagacttt
     1681 gtttatatag atgatgtaat ccaagcatta cttttagttg cgaatgagga agctgcactg
     1741 gggaaacaat tcaatgttgg aacgggtaaa gcaaccacac ttttggaatt gattcatgca
     1801 atcaatgaaa ttctcggtgt agaattgaca cttgaataca aagaagagcg ttcgggtgat
     1861 atccatgact ctctagcaga tattactaag atccagtcaa ttggctatca accgaaatat
     1921 ggtattttaa gtggaatgac taattatttg aaaactgaaa tcaattaaaa catactaaga
     1981 taagattaga gggtaaattc aggctctttg tcaataagga ctgatgagtt ttgtaaaatc
     2041 aaatctggga tagaa
//
LOCUS       DAYYKN010000236         2003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000236 DAYYKN010000000
VERSION     DAYYKN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.243"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..425)
                     /locus_tag="ACHBB7_002881"
     CDS             complement(<1..425)
                     /locus_tag="ACHBB7_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM9210761.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="ACHBB7_002882"
     CDS             complement(534..1565)
                     /locus_tag="ACHBB7_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9210762.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          618 a          328 c          347 g          710 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtagga gcaattatat tga
//
LOCUS       DAYYKN010000237         1991 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000237 DAYYKN010000000
VERSION     DAYYKN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.244"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACHBB7_002883"
     CDS             <1..963
                     /locus_tag="ACHBB7_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9210763.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="ACHBB7_002884"
     tRNA            1152..1225
                     /locus_tag="ACHBB7_002884"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
     gene            complement(1738..>1991)
                     /locus_tag="ACHBB7_002885"
     CDS             complement(1738..>1991)
                     /locus_tag="ACHBB7_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9210764.1"
                     /translation="RFVKDFYKVWDSEVFFLAIEDSLLINLYYSNKNYFKIPAAKTRM
                     KKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          663 a          350 c          366 g          612 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaat
     1261 tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa tgagtggctt
     1321 ttttatgtat ccttttatgg attaatgaaa ggatgtttca catagttata tttctgtata
     1381 tttgaaaagt tttactttga tttttaaata gaaagacatt tgggttaaat tgtgagatga
     1441 taataaagaa gagtttaaag cgcaccccaa gccacttccc cataagtgtg ttacgcttta
     1501 aactctttta tatttgaagc tattaaaagg cataccatat ttttgaaaaa aagtgagaaa
     1561 aaaggcttac aattggagtg gtagttaatt agtgacttat ttttgatttt atagcactga
     1621 tactataaaa tatagatatc atcatattac acaatcttaa tactaactta aaaaatatct
     1681 cctttcacaa gtatggtgat aaaattcgtt ccgggctacc tttttaggta gcctacttta
     1741 atctttgtat ctttctggat caacgaaagt atactttata tagtcataac gccgatgatc
     1801 gcttcgagcg tctagcacat cagtcacgat atcaaacaaa aaatatacgt ctttcttcat
     1861 tctagttttc gcagcaggga ttttaaagta gttcttatta gaatagtaga gattaattaa
     1921 taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt
     1981 gacaaatcta t
//
LOCUS       DAYYKN010000238         1979 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000238 DAYYKN010000000
VERSION     DAYYKN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.245"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..367
                     /locus_tag="ACHBB7_002886"
     CDS             <1..367
                     /locus_tag="ACHBB7_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM9210765.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..749
                     /locus_tag="ACHBB7_002887"
                     /pseudo
     CDS             567..749
                     /locus_tag="ACHBB7_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1330..>1979
                     /locus_tag="ACHBB7_002888"
     CDS             1330..>1979
                     /locus_tag="ACHBB7_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9210766.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVDDK
                     VHPLKLSWSIDRITIVGKLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQ
                     LFDQYGEDIAYIEMLKFHPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIA
                     CDILGVPDDFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          657 a          305 c          461 g          556 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggtttaact
      661 tggggtttag tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaatg gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcgttaaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacga tcgtgttaga ttgtgaaaac cgatagctga atgaaataaa cagcgggtgc
     1021 gccctttcca tttcggcagg aggaggctca aggggttcgg ggaatgaaat tccctgaggt
     1081 ctttttattt ttttaggagg agtgctataa tcaaagaaaa cgagaaaaaa gagcaaaata
     1141 aaagccgagc agtgcgctaa cactctcgac tatttacgga aactacttca cctagctccg
     1201 taactcaggt tcattatacg aatattttta ggtttcttca agctagagat ttttggatat
     1261 gaatttttag ggttacgtga actctggaaa ttctttccaa aagtctcttt tttcgtctgg
     1321 aggacgaaaa tggcaaatga aaatcagttg agtgaaattc aatcaaaaat tgaaggtcga
     1381 ggggcagaaa aaagaacaac gataaatcag ctcgaaaaac ttaaccgtat ttctaacagg
     1441 cggttaagtg ttgacgataa ggtacacccc ctaaaattaa gttggtcaat cgatcgaatt
     1501 acaatcgtcg gaaaactgaa tgataccata gcttattgga cgcaaaatgg agttgtgttt
     1561 gtcgattttg agcaattaat gcgtttgaat gaaaatcaag ggtatttgaa gtcggttggt
     1621 acaaatggtt ggcaattatt tgaccaatat ggagaagata tcgcctatat cgaaatgtta
     1681 aaatttcacc ctgacaaagg tcgtattgat tttaatccaa ataaaattgg cgagttttta
     1741 aaggggtcga tgaaacaatt cattcatgat ttgtttctgg atgcgcattt ctcacgtgca
     1801 gatattgctt gcgatattct cggtgttcct gatgatttta ttacccagta tcgaatcgtt
     1861 gaccctgtgt cttttaagcc aatttatggc cgttctggca aattagaaac agcgtactgg
     1921 ggtagtcgtg ccagcgagcg acaagtgcgg atgtacaaca aaaagcttga acaggaacg
//
LOCUS       DAYYKN010000239         1966 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000239 DAYYKN010000000
VERSION     DAYYKN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.246"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..946)
                     /locus_tag="ACHBB7_002889"
     CDS             complement(107..946)
                     /locus_tag="ACHBB7_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9210767.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1131..>1966
                     /locus_tag="ACHBB7_002890"
     CDS             1131..>1966
                     /locus_tag="ACHBB7_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM9210768.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLIDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VM"
BASE COUNT          755 a          376 c          302 g          533 t
ORIGIN      
        1 tctaacaaaa gatgtcgtgt attcgcttga atttgtttac tttttcgttt aatcaatgac
       61 agacttaaaa aagcaagacc gatatcagcc ttgctttctt catcttttat tgataaacag
      121 cagtgtattt cttcatccgc tttgttttat atgcatagag taacaaacca acaatcaaga
      181 ctaacaagaa taatgtagtt gatatagatg cttcagcccc aaagtttccc ccagtaatta
      241 ttgtattttg cgattggatc agagtggaga aaaaaccttg ttcttgcgtt ccgctgactg
      301 gtatacccaa aatagctcct tgtgtccaat tccaagtaat atgagcacca atagccgtaa
      361 ataaacttcc tgtactgtag ataagtgcgc tgaaaaagca acctgctaaa aagatattga
      421 aaactgccag aaatgtcatg ttaggattca atccgtgcaa aagagcaaat aatgcagcag
      481 atccaatcat tgctattaac ggaccaaatc taagcatctg atgctgaaaa aagccacgga
      541 ataaaagctc ttctgatgcc cccactgcaa taaataaaat aaaactcgtt atcagcggca
      601 caatgtttgc gtttcctgaa tctaaccgat acgcccccgt caaatataat ggactagtaa
      661 taagcagaaa cactccaatt ccaacaacga gacctcccgc taaatacccc cagtcttttc
      721 ctcgaacaga aacaccataa tcttctattc tttttccttc gaccttacta gcaagaaata
      781 aagctagtaa aaaaacagct aaatgtccga ccgtaccggc aattgctcca atccattcac
      841 tatttccaat aaacatatcc gcaagtgcat tattgacaag aattcctaaa acaaaaccaa
      901 ctaagactat taaaacagat ccaatcaacc gaataatcct gatcatcttt catccctcct
      961 ttatattaag tttaacaacc cttttctttc ttgtctcgtg aaaactatgt aaatgttctc
     1021 catcatagag agaatagatt tttacgaact aagaatatct acagcaacct tacgaaaatg
     1081 aaagactgtc tccctaattc ttctgttaga ctgtttttaa taaggaaagg atgggaaatt
     1141 ttttgaaaaa acaactacta attatgtgcc tttcagcatg tgcaatttta ctattctcag
     1201 gttgtagcaa gtccaatgaa acgattgcaa cgtttaaagg tggaacactt acagttcagg
     1261 atttttatga taacccaaaa gttaaatcaa ataatcaaac actattaaaa aaaatgattg
     1321 tcttcaaagc atttgatgat gtatacggaa aagagatttc ttcaaaggaa gtaacaaaag
     1381 aatataatga acaaatcaaa aaactcggtc caaactacaa ggaacaatta aaagcagttg
     1441 gccaaacaga agagacatat aagaaactct ttaaacagat gctggctttc caatacggtt
     1501 tgaaagccaa tgtaaaatta atagataaag atctagacac tgcttggaaa gaattctatc
     1561 cagaagttag tacacagatc attctatttt cgactaaaga agaagcagat aaagcaaaaa
     1621 aagaagcaaa tgaaggagaa aatttctcca agcttgttca agcttatgga aaaaataaaa
     1681 cattaaagga aactgatgga aaaatgaatt ttgattcaac caatccagag attcctactg
     1741 aagtaaaaaa agcagcattt aaactaaaaa acggggaagt ttctgatatc attcctgtaa
     1801 cagatccaac gacttatcag caaagctatt atctagtaaa aatggtaaaa aaacaggata
     1861 aaggttctaa taaagacaaa tacaaatctg agctagaaaa aattgctacc gaagcaaaaa
     1921 ctcttgatac ggaattcatg gataaaacaa tccgtaaagt gatgaa
//
LOCUS       DAYYKN010000240         1954 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000240 DAYYKN010000000
VERSION     DAYYKN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.247"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            679..1905
                     /locus_tag="ACHBB7_002891"
     CDS             679..1905
                     /locus_tag="ACHBB7_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9210769.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT          713 a          283 c          404 g          554 t
ORIGIN      
        1 ggctaagtaa atgcatttgt tcacttgaca tgtcctaact aattatcaag caaggaacat
       61 tttcttcgtt agtaatggat tttaaagaaa tttcagtttt tggatgttga ataaaagacc
      121 atttgtgaaa ccactagtat acatactcat taccatgtca attctattac tttaaaagaa
      181 aatcgtttta ctttgaagag gagcatatct atccattaaa agatgatggt aatgcaatca
      241 ctagttcgag tcattttaag agccatgcag tgagctggaa ataaaacgaa tagaagatgg
      301 gtttaacaaa aggaccagtc ataggaagaa attgtcgtga aatcgaatga taatctgaac
      361 ggtaggcaaa gactgttaaa aagatgggaa aggtatgtgc gatcatttta ctgcacgatt
      421 atttggaaaa taccttgttg ttcgcttttt ctggaaaaaa tacataaaaa aagataaaac
      481 gattgcgaac tgcgtgtatt cggtctttcg atctttttca cggtatggta tgatagttaa
      541 gaattttgag aaattttaaa aaataataat cgttacacaa aaatgttttt taaagttttc
      601 ttatgattaa aaagtgaatt tgctgattga acagttatat gagacaatat tttagaaaat
      661 gtgaaacaga ggtgtattat gaagcgatgg caaaaagttc tattaacctt gttaggggta
      721 ttcgttttgc tgattgccgg attatcagca tacggaatca gatttatggg agaagcgagc
      781 caaacagtaa acaaaatttc taaaaactct aatcgaatat cctccaaacg ttctgagaga
      841 gtaagcattg atgacaaaga acctttttcg gttttgctat taggtttaga taccggtgga
      901 ttaggaagaa cggaacaagg acgttctgat acgatgatgg tagtaacagt taatccgaaa
      961 caaaaaaaat caacgatcat cagtttggac cgagatattt atacgaatat cgtaggttat
     1021 ggaacggtag ataaattaaa tcatgcctac gcatttggtg gtgtcgaaat ggccatggat
     1081 tcaattgagc aactgctcga tattcctatt gatcattatg taacgatcaa tttggatggc
     1141 atggaagatt tgatcaatgc ggtcggtggc atcaaagtga ataataaaat tgactttact
     1201 ttggatggtg tccatgtacc agtaggtgaa caaacattga atggagaaaa aggtttagcc
     1261 tattctcgaa tgcgacacga agatccagaa ggcgatatcg gtcgacaacg tcgtcagcgg
     1321 gaagtagtga cgaaaatcgt caataaagtt ttaagtttag atggcgtaag caattataga
     1381 aaaattttaa aagcagttga gaacaatgtg acgacggact tagattggga tgacatgctt
     1441 gatgtagcga cgaactacac cccggcgttt gaaacaatca aacaagacca gttgcaaggc
     1501 gaaggacaaa tgatcaatga tatctactat caaattttag gaaaaaatga tcttctttcg
     1561 atccaaaatg aactgaagaa acaattaaat ataaaaacaa gcgatacatt acctaatttg
     1621 aaaaatgaga atgcttcaat gatgttcttt gatgattcgg aagatggcga ttcgacaaac
     1681 ggtgatggat cgaatagtga agggtacacc gattattctt cctattcaga aaacacatat
     1741 gatcagtacc aagatccgaa tacctattat cagcaaacag aagatacaag tcagtactat
     1801 acaccaagtc aagatactca aagttatgat ccttacgctg gacaggaaca aaatggttat
     1861 ggttatgacc agaataatga gtatggaaca acaagtgggt actagaaaaa aatagtttgg
     1921 ttaataaaga gtgccaccta aattagacaa aaaa
//
LOCUS       DAYYKN010000241         1949 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000241 DAYYKN010000000
VERSION     DAYYKN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.248"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHBB7_002892"
     CDS             <1..1087
                     /locus_tag="ACHBB7_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9210770.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1294..>1949)
                     /locus_tag="ACHBB7_002893"
     CDS             complement(1294..>1949)
                     /locus_tag="ACHBB7_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM9210771.1"
                     /translation="ENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKR
                     LMRFIFVTGVRSEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRII
                     SLDRQTVEDLKTWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHM
                     HGLRHSHVAFLIEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
BASE COUNT          665 a          347 c          336 g          601 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa tccatgtaaa catggtttat
     1141 tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc gtccattttc
     1201 gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa aaataggcta
     1261 tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg taatttcatt
     1321 tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag tagttttaat
     1381 agaggcatga cctagacgct tagatattgc atagatattt ttattatgtt caattaaaaa
     1441 ggcaacatgc gagtgtctta atccatgcat atgaattggt tttactcctg caatttcagc
     1501 tagttttttt attctgctta aaatagttct tggagatggc ggtaatccat caaaagaaaa
     1561 aaccaattct atatttttcc caattgattg ttgagttttt ttccatgttt ttaaatcttc
     1621 aacggtttgt ctgtctaacg aaattatccg aatacttgat gtattttttg tttcagcaaa
     1681 ttcataatct tgtcttgtac ggatgtaaat tgacttattt acgctacatc tgccatttat
     1741 taagtcaaag tctttccatt gtaacgctaa gagctcctca ctccttacac ctgtaacaaa
     1801 aataaaacgc atcaaacgtt tataaaattc ttcataaaaa tccccgtgat aaacactatt
     1861 atatactttc ttaaattcat caactgtcca aaattctact tgaggacgct gagtttttat
     1921 tttacctata gtatctacag gattttctt
//
LOCUS       DAYYKN010000242         1937 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000242 DAYYKN010000000
VERSION     DAYYKN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.249"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="ACHBB7_002894"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="ACHBB7_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM9210772.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="ACHBB7_002895"
     CDS             853..1041
                     /locus_tag="ACHBB7_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM9210773.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="ACHBB7_002896"
     CDS             1054..1611
                     /locus_tag="ACHBB7_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9210774.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="ACHBB7_002897"
     CDS             1643..1885
                     /locus_tag="ACHBB7_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9210775.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          714 a          298 c          398 g          527 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgta
//
LOCUS       DAYYKN010000243         1874 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000243 DAYYKN010000000
VERSION     DAYYKN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.250"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..633
                     /locus_tag="ACHBB7_002898"
     CDS             133..633
                     /locus_tag="ACHBB7_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9210776.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..>1874
                     /locus_tag="ACHBB7_002899"
     CDS             730..>1874
                     /locus_tag="ACHBB7_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM9210777.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQ"
BASE COUNT          598 a          257 c          427 g          592 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaat
//
LOCUS       DAYYKN010000244         1824 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000244 DAYYKN010000000
VERSION     DAYYKN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.251"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..768
                     /locus_tag="ACHBB7_002900"
     CDS             <1..768
                     /locus_tag="ACHBB7_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM9210778.1"
                     /translation="KEFSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQA
                     TSAAETIAEQLKRLSVDEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAG
                     NEKYRFITLSSIEHFPLTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVI
                     VDRIRKNKYKNRRPSIFHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTM
                     EEMAFLKRQIFSELSKKFIPHAFSYIKEYGTVLVSKT"
     gene            902..1027
                     /locus_tag="ACHBB7_002901"
     CDS             902..1027
                     /locus_tag="ACHBB7_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM9210779.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1161..1793)
                     /locus_tag="ACHBB7_002902"
     CDS             complement(1161..1793)
                     /locus_tag="ACHBB7_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210780.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT          697 a          272 c          345 g          510 t
ORIGIN      
        1 aaagaatttt cgcaaaataa attttcccat tttcttgatt tattgatcga tgcaggatta
       61 atcaagaggg aagaacgaag atatcatttg aactttccaa tatttgatcc taaagattac
      121 ttgcaacaag ccacttcggc tgctgaaacc atagctgaac aattgaagag actctctgtt
      181 gatgaacaaa aattagcgat gggagaagtt atttgggcgt attgtttcga agatgaacgg
      241 aaagaagcgt atttttatgg agtaagaaac agccgagaga cagaattgct gaggacaact
      301 gcaggaaatg agaagtaccg ttttatcaca ctttcctcta tagaacactt cccgcttact
      361 ttggctaatt acttttttgt acagaagaat cagcttcctg taacgaaggc attcaaagaa
      421 ttagccgaac ttattggaga cgtaaatgaa tcgtactttt ttgatcaaat agaagttatc
      481 gttgacagga tacgtaagaa taaatataaa aatcgccggc ctagtatttt ccatcagtct
      541 ctacttgtga cgaatactat aaaggaagaa gaatcattca cattagaatt acctattgtc
      601 gaaaaaaaca attttgagat tgaattacca acattggatc cttcattgac aatggaggaa
      661 atggcctttc ttaaacgaca aatcttttct gagctgagca agaaatttat accgcatgca
      721 ttttcttata tcaaagaata tggaacagtt ttagtgagta aaacctaaga gagcagtaag
      781 caaaaattat actgactctt gcaggaggag cctttctctt cctttatggt taaaagaatt
      841 atttgagtat tttgcctatc taccacctat tagattcact acatggtctg gaaacaaagg
      901 aatgcaaaaa aatggtgaga aatgtggaat gacaaaggaa gtagtgataa gaaaagttag
      961 atttttaaat aatcaatatt atgattcagt caagtatggc attttatggg aagaactagc
     1021 tgattagatt aggaagtcca tttgataatc cagaatcact aagaaacgaa tggcgagtca
     1081 ttaataattt atccaatctt gggttattct tttagattga agtgtttaaa actaaaaaag
     1141 catttatgaa gtcgtaatag tcaggcttca aaatgctttt tattttgttt gttttttctt
     1201 tgccacataa atacacctag actgacgatg aagagcatat ccaacagatt tcctgcgact
     1261 tggatcgtcg aagggttggt cagtggcatt aaagcaagga caaaaaagga aaacaagctg
     1321 taaataaaac aggtgacagg aactaatcga aagaagagcg gacatttcaa ttggctaaaa
     1381 atgagtgaaa aaataaaata agtaaaaatg gataaaatca cgactaaacc attcatgata
     1441 aagaagaaaa gcgtattgtg attaaacgaa taaaaaagaa ttgctaaaaa agaaaacgtt
     1501 ttaaatgcaa tcaatacgct tgaagcttcg tttaacgtac caggatgtcg atgaaaaaaa
     1561 taggcaccga aataaacaga aaacagtaag ccgcaaagag aagacaaaat caaaaaaaac
     1621 ggattttctt cggcttctgt gatatcagaa tgatccaaaa gcaaccagcc taatgcacta
     1681 gaaggcgcat gtgaggtctt aaggtatgaa taacatagaa aaaataagat cgtcaaagtt
     1741 gcaaaaaaag agcctaaatg taaaaaatta gtggttctac gttgaatttc cataatatct
     1801 ccaaattaaa ttgataagta tata
//
LOCUS       DAYYKN010000245         1773 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000245 DAYYKN010000000
VERSION     DAYYKN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.252"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACHBB7_002903"
     CDS             <1..1604
                     /locus_tag="ACHBB7_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM9210781.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          641 a          285 c          359 g          488 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaagtgta gaaaataggg atagatgtct
     1681 ttttacttgt tcacatttta taacggagta tttaagaaag ctattgaata atgattcttc
     1741 catctttttc attcatttac cggttctgtt gca
//
LOCUS       DAYYKN010000246         1752 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000246 DAYYKN010000000
VERSION     DAYYKN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.253"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            383..1633
                     /locus_tag="ACHBB7_002904"
     CDS             383..1633
                     /locus_tag="ACHBB7_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM9210782.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          578 a          335 c          309 g          530 t
ORIGIN      
        1 ttgtctaata gtttttattc aaaatgtttc tagattataa aatttattga ataacacatt
       61 cctaacggaa tttgtttcga tattgtgtga tttctcagag tacaatggat gtatggaaag
      121 gatccaacgg gaatttgcta tttcctcctg tagcgtttgt ctaccctgtt tagtctgtta
      181 cccgaaccac tgttatcact accaactcat tagctgtttc gataatggac tagactttca
      241 aatagtagtt tggaaggcag cttctagctc ttttatcacc gtattctatt aggcgaggtt
      301 gtggctatac agtgagtttc tgggaaatcc cacagtatcg aagcattttc caaaatttat
      361 atcgaaagga ggtccttctt ccatgaaatt atttgtcggt attgacgtta gctctgaaaa
      421 actagatgtt tgttttttaa cagacgacaa tcaattgtct atcttatctg aaatctctgt
      481 tgccaatgac atcgaaggtg cttcattcat acgagaaaca attcttgaat tcaataacag
      541 ctaccacttt gatcaaattg tgattggcat ggagtcaacg tccatgtaca gcttccatcc
      601 ttccatgttt tttcatgaag atgagaaact aaagaagctc aacacacttg taacgattga
      661 aaatcctttt cgggtgaaac aatttagtcg catgtttgat gagaataaaa ctgatcgaaa
      721 cgatgcatta agaatcgctg atttcttgcg tattcaacga ttcacaactt cccctatcaa
      781 agaagagaaa tacatggcct tacagcgttt aacacgtact cgctatcaac ttattaagca
      841 attgattcgt acaaagcaac attttcttga aaatctaacg tacaaatgta atacgcttgc
      901 tcgagaaatg cgagacgaat cgacgagtct ttttagtgca accctcattt cactcatgac
      961 ggaagatttc acattggatg aacttgctga gcttccttta gaagctttct gtgatttact
     1021 tcaagaaaaa ggaaaaggcc gtttcaagca accagaaaaa atcgctaaag caattcaacg
     1081 tgcaattacc atgagttacc gcctaggtgc tcttgcacaa gaatcaatta atgttgtttt
     1141 aagtgttctc gtacgagaaa tacgagcact tgaaaaaaat atcaaagaat tagataaagc
     1201 catcgaacaa gtaattgttg tcattccaga atatcaatgc ttaaccagta ttccaggcgt
     1261 tggtaaagtc tatgctgccg gtttaatcgc tgaaattggt caaatcgaaa gatttgaaga
     1321 tcagacaaaa ttggcaaaat acgctggatt aagttggaaa gtgaaccaat ctggaaacta
     1381 tcagtcgcaa aatacacctc tgacaaaaca aggaaatcga tattttagat actacttagt
     1441 tgaagctgcc aactctgtaa aaaattatct tcctgaatac aaagcttttt atcaaagtaa
     1501 atataaggag gttccaaagc atcaacacaa acgtgcactc gtcctaaccg caagaaaatt
     1561 tacgcgtctg gtggatacgc tactacgtaa acaccaactc tatacgccac caaggagtgt
     1621 gatagagaaa taacaatccg ttattgacgc aaatccttga atcaacccga aaaaaatttg
     1681 attttgatcg ggtctagttt cgtgcgcttc aaaacatatt actcaataaa gaaaatctta
     1741 aatttcatct tg
//
LOCUS       DAYYKN010000247         1721 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000247 DAYYKN010000000
VERSION     DAYYKN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.254"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..585
                     /locus_tag="ACHBB7_002905"
     CDS             19..585
                     /locus_tag="ACHBB7_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9210783.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            730..817
                     /locus_tag="ACHBB7_002906"
     tRNA            730..817
                     /locus_tag="ACHBB7_002906"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:762..764,aa:Ser,seq:cga)
     gene            1151..>1721
                     /locus_tag="ACHBB7_002907"
     CDS             1151..>1721
                     /locus_tag="ACHBB7_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM9210784.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          583 a          267 c          340 g          531 t
ORIGIN      
        1 agaagtgggg gagttttaat gagaagaaaa gtctatacca aggatcaaat tctaaaagct
       61 gcttatgatg ttgttgcaaa ggaagggttt aaaggattta ctgcgagaaa tattgctaaa
      121 aagatgggga tttcgacaca gccgatttat ctggaattca aaaacatgga agatttaaaa
      181 aatacgttgc tggattctat ctttgaacaa ctaaaagaag atgttttccc tattgaacgt
      241 actggtgata aaatcattga tctcggtttg aactatatct actttgcaaa aaagcatcat
      301 agtctttaca tggcattgta cattgatgaa catggggacg gaaagaaaat gcatgatttt
      361 tcatacaatt atttccgtga aatagttaaa gaagatgaaa gatacaatca attatcggat
      421 gaatatgtcg atgctctgca tactggtaca tgggtagcag tgactggctt agcgtcattg
      481 atgtcatcaa acatcatgca tccaacagaa gaacaaatca ttgcactgat caaacggaca
      541 attgactcga ttttagcatt agataatcct gaaataatca gatagtgttc tatcagtatg
      601 aatttgatcg gtaagtccgt tggctgcaaa ctgtcaatgg acttttttgc taaaaaaagc
      661 gatttctgtt atttatctat tagaaatagg cttgacattt cgtgaaaaaa gtttaaaaat
      721 agtacttacg gaaggttggc agagtggtaa tgcaccggac tcgaaatccg gcgaaccggc
      781 taataccggc gcgcaggttc aaatcctgta ccttccttat atcaacgatt agaggttgtg
      841 aagaagctgt cctgcatcaa gtaataagaa cggccaaaca aaaatagctt ttcctatttt
      901 ttcgtttgat tgggcttatt accgcagatg caagcttttg aacaccgttt gttcggaata
      961 aggggctgtg acaagacttt tgtcacagcc ccttattgtt gtacttttaa cctacaatat
     1021 tttttgattc cccctgagta ctatttttta taaatgctag taaatcaatc ttctttggcc
     1081 tttttttgat aaaaaaaata ctagatgtaa gataaaagtg taaaaacgat ttgttttagg
     1141 aggggaaacg atgtgtaccg caatcactta tgcaacgaaa gaccattatt ttggaaggaa
     1201 ttttgattat gagatgtctt ataatgaagt ggtcactatt actccaagaa attatcgttt
     1261 cgattttaga aaggtaaaaa atttagataa acattatgca atgataggaa tagcagctgg
     1321 ggtatccaac tatccactct attatgaggc tacaaatgaa aaagggttga gtatggcagg
     1381 actgaatttt cctggcaatg ctgattataa agaattacaa gaaggaaaag ataacgttgc
     1441 accatttgag tttattccat ggatactagg gcaatgctca accatagacg aagctaaaga
     1501 attattggct actatcaatc tagtcaatat cgattttagt gaaaaactac ccttgtcacc
     1561 tttacactgg ttattagctg ataaagaaaa atcgatagta attgaaagta tgaaagatgg
     1621 acttcattta tatgataatc ctgtcggtgt gttgacaaat aatccgccat ttgattatca
     1681 attatttaat ctgaacaatt atcgctcgct atcaaatgga a
//
LOCUS       DAYYKN010000248         1681 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000248 DAYYKN010000000
VERSION     DAYYKN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.256"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..51)
                     /locus_tag="ACHBB7_002908"
     CDS             complement(<1..51)
                     /locus_tag="ACHBB7_002908"
                     /inference="COORDINATES: protein motif:HMM:NF040897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM9210785.1"
                     /translation="MGLKFERTDNLDKLFES"
     gene            187..1515
                     /locus_tag="ACHBB7_002909"
     CDS             187..1515
                     /locus_tag="ACHBB7_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210786.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT          561 a          279 c          311 g          530 t
ORIGIN      
        1 tgattcgaaa agtttatcta aattatctgt acgttcaaat ttcaacccca ttgtaaacac
       61 ctctttgttt ctttttactt agtctatcat gtaatcgtcc aaattggttt ctaagacata
      121 aagttttagt ttttctttat cgttggttat aattaaatta taaaaagatt ttaaagaggt
      181 gagaaaatga ttggcgaaga taaattattg atgactatcc agctcacaga cttaaatgaa
      241 gaagaaaaaa aacaggaaat aagcgattgg gctattttga cacgtctgtt gatccagcca
      301 acatttatcc gctctttcac acaacaagcg gatccgtatg atcttcgaaa gcttcaagaa
      361 aaaataatgc tagcatcggt aagagacgaa agttggttag ttgtaggaaa tgacgagaac
      421 gaatgtagtt ttcgcttgga agataatcaa ttattgataa aaaatgtatt gtctatctct
      481 gttttcaaag aaaagcaatt tttgattcga gacttcattc agaaaaaaat gattgctcat
      541 ggggtgttcg cctatatgcg agcttatagc gagtttatct atcacaatac aaaacagatc
      601 tctcaacggc tattgtttga gaaaaaggat gaaatcgaac atttacctaa aatgaaacaa
      661 caaaacgggg aagtagtagt cgattgcaac caatttccag gatacgatct tttttaccaa
      721 ggtttatgtt tcacttcgtg ttgggaaatg tattatagtc gctattatca tcaaatcatc
      781 cctaaaccga tttttctcga catccaacaa gtagaaaaag tgaaggaatt agaaaacgaa
      841 gtcatatgta tccagcttta tcgagacccc tttaactggc aaaagccaaa caatcaaatg
      901 tttcaatcct attttaggga ccagcttggg tttgatcatc ttgcttggga caatggtgtc
      961 ggtttattga aaccgccatt cgttgaatac atttacactg accatacgat ccaatctgtc
     1021 cagtatcaga atgcacaaat gcaacctgtt cctaaaaaga atgcttcatt ttttgtaaca
     1081 aaaagttatg atattcaaca aggagattat aaagaaaggc gtgttcgcgg aacactaaat
     1141 gctcaggcgt attttccttg ggttgacgag aatcgctcgc gcatgatgtg ctataaagtt
     1201 atcgatccga ctgttgcttt ggataatgga atcgaagctt attgttatta tattagggaa
     1261 tatctagaag tagaggtaac agatgaaaaa tatcaagagt atcttttatc cttaagaatc
     1321 tatgttcctt cagaatccct gtcagaatta ccgttaaagg aaatcaaaca tagactctct
     1381 gatgttacct tcaaacgaat aaagaaaaag cggcgaagta tccaattcga tgtcaagaaa
     1441 ggtgagcagc atctgcgtgt gcaattttta gattaccgag aattagaaca attgattact
     1501 ttacaaaaaa tatgaggtga agaaaatgga taaacaactc tattatcgaa taatggttcc
     1561 ggcatcatta ttatccgtct gtctcttttt tgttcctaat tttcctattc gatcagtcat
     1621 cggttttttg gtcatgtttg gcgggatggg actttattat tatcttcttc ataaggaaag
     1681 a
//
LOCUS       DAYYKN010000249         1676 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000249 DAYYKN010000000
VERSION     DAYYKN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.257"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..402)
                     /locus_tag="ACHBB7_002910"
     CDS             complement(<1..402)
                     /locus_tag="ACHBB7_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFM9210787.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIE"
     gene            complement(407..592)
                     /locus_tag="ACHBB7_002911"
     CDS             complement(407..592)
                     /locus_tag="ACHBB7_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9210788.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(946..1191)
                     /locus_tag="ACHBB7_002912"
     CDS             complement(946..1191)
                     /locus_tag="ACHBB7_002912"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210789.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(1185..1586)
                     /locus_tag="ACHBB7_002913"
     CDS             complement(1185..1586)
                     /locus_tag="ACHBB7_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9210790.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT          530 a          312 c          216 g          618 t
ORIGIN      
        1 ctcaatgccc ttttttacaa catggatttt cgataaaaac tgttgaaatt cctgtatatg
       61 aattggtcga atctttacaa cctgcatttc tccaaaaaaa tccaatgcac gttgtaaaat
      121 atgatctgtt ttcatatatg ttttttctac agttcccaac ttataccaag gaaaaaaata
      181 ctctcgataa aaatcatgaa acgttattcc ttgcagataa attccttgtg aatcagaaaa
      241 actttgtttt aactcttcga gtccccttct agcttctcct tgagttttga agcctcttct
      301 ggtcacttga attctttttc caaagcgatc ttgtcctaaa gttgcagaaa aaaaccaagt
      361 gcctcttttt ctgtctttat aaacattcgc aattttcctc attttactat tcctcccaag
      421 aaatatggag aacttcttct aaaattgaaa taggaattct ttttattttt gaattacgat
      481 aaaaaaagta tcctttctca actaaaagtt ctttacattc cttgaagatt ttattagcat
      541 agtactgaga aaaacctaga ttaatcaaat cttttcgagt agctgttttc attatagcac
      601 cttccttact ttttttcatt tatttctatc agctgctttt gtcaaaagca cataggaatt
      661 tcacctctcc ccaagtttac ggcgagcctt acggaagtat cattatccca actttaaatc
      721 atcgcttttt attgactata aaaattattt tagaatattt atcgctcctt ctttacagca
      781 agaaatcatg gcgcaactaa aatatgctcc ttaaagtgtt aacgtactga tagaatctat
      841 ttaattttca atgaacaata ggttttctac ctaaaagaat ctactaattc cttaactatt
      901 agaagattct tttaaacaga aaaagtggaa aggggatttt tttaattagt taattttaaa
      961 ttttaagatc gcttctatta atttaatttc tagctgactt atcatatatt catctacata
     1021 ggtaacgaat tgacctacct cgttaaatcc atttgtcaaa cataacttag ccatataaga
     1081 atgataatgt ttgagtatca tcttgatcga aattggatct ccttctagag cagataagat
     1141 aatggatagt ggaactaata ggtactgccg ctttttagat ttatctacca taggtcatac
     1201 cccattttca attgttcaac tgccttcttt cgttgatacc agataccact tgtgctcata
     1261 ttgagaattt taccgatttc agcatcatta aaacctgcaa aataataaag taggataatc
     1321 tttcttctta tctccgataa ctgatcaatt gctgctgata acttttgatc tgaaattaat
     1381 atttccattc caaataagaa atagacatct tgttcatcaa aatgagtttg ggaatacctg
     1441 ccttcatttg aatctgccaa tggttcctca ttatccttta tgttagcgtt tcgttttcga
     1501 atagacttag cttcgtttcg taatactttt ttacagtagg catcaaattg gtgttgtata
     1561 gatatgtcat tgtaatgttg ttgcatgata atatgctcct ttttccccct ttccactaat
     1621 acaacaatca gaaagcccct ttttttcgaa aaaactagaa aaaaaacata aaaata
//
LOCUS       DAYYKN010000250         1664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000250 DAYYKN010000000
VERSION     DAYYKN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.258"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHBB7_002914"
     CDS             131..505
                     /locus_tag="ACHBB7_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9210791.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHBB7_002915"
     CDS             complement(619..1248)
                     /locus_tag="ACHBB7_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9210792.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHBB7_002916"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHBB7_002916"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAYYKN010000251         1657 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000251 DAYYKN010000000
VERSION     DAYYKN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.259"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..403
                     /locus_tag="ACHBB7_002917"
     CDS             5..403
                     /locus_tag="ACHBB7_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM9210793.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="ACHBB7_002918"
     CDS             387..1082
                     /locus_tag="ACHBB7_002918"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFM9210794.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          625 a          244 c          325 g          463 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccc
//
LOCUS       DAYYKN010000252         1639 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000252 DAYYKN010000000
VERSION     DAYYKN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.260"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            415..>1639
                     /gene="ltrA"
                     /locus_tag="ACHBB7_002919"
     CDS             415..>1639
                     /gene="ltrA"
                     /locus_tag="ACHBB7_002919"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM9210795.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRL"
BASE COUNT          585 a          299 c          388 g          367 t
ORIGIN      
        1 acttggtggt gaaagtccac tacaggcttg gcagtaggaa ctgttagcga aagacaaggc
       61 ggcaatcgtg aggttgtggc tgaaggaagt cggacgcaaa ctcctgaact gacgaacaga
      121 aactagatac aaggctgaat taggtcggat aagtcttcat cacaagacaa agtccaatac
      181 tgcccgaaac ctatacagta aatctagcag ttacatggga ggaaagtttt cactcttacc
      241 gggggagatc tcatgagggt gttgtgggcg ttgaatgaaa acaaaccagc acaacgatca
      301 tggcgacatg atggtgaatc atgagaagtc agccgaggtc atagtagtaa cgacactgac
      361 cgttacgaag gactgaacaa taagaatctt gaataattta ggaggtgtga gaagatgcga
      421 cgaccgcaga aaacaagaaa acttggctat catcaaagag ctagtgtgga acacgaaggg
      481 tatgatgaag tgcgcagcac ttcttgcggt gaacagagga aacaagatgg tgtagaaaat
      541 ctgtttggga gaattctaga tcggaataat ctcaatcaag cgtatcttca agtagtcaga
      601 aataaagggg cggctggtgc agatgggatg accgtcgatc aactgcttcc ttacttgaaa
      661 gagcaccgcg aggaattact atccaattta cgccaaggga actataaacc tcagcctgtt
      721 ttgcgagtag aaataccgaa accaaacggc ggtgtaagaa aactaggaat tcccacggtt
      781 attgatcgta tgcttcaaca ggcaatcaat caagtgttac aaccgctatt tgaaccagaa
      841 ttctcagata atagttatgg gtttcgccca aatcgcagcg cacatgacgc agtgaagaaa
      901 gcaaaatctt attatgaaca aggttaccga tatgtggtgg acattgatat gaaagcctac
      961 tttgacaccg taaatcacga taagctgatg tatttcattg agcggaaagt aaaggacaaa
     1021 caagtcctaa aactgatccg tcaatactta atgagtggtg taatggaaaa tggactcatc
     1081 acaacaactg aagaaggaac gccccaagga ggcaatctct ctcccctgtt aagtaacatc
     1141 tacctgaacg aatttgatca agtgctagaa aatctaggac ataaatttgt tcgatacgcc
     1201 gatgactgca atatctatgt tagaagcaaa cgagcaggac agcgtgtgat gaagaaatcc
     1261 attcaatttc tcgaagaaca actaaagttg acggtaaatc gagaaaagag tgcggtagga
     1321 agtccattga aacgaaagtt tcttggattc tgcattctgc caacaaagaa aggtattaaa
     1381 attcgccctc acgcaaaagc aaaacagcgt gtgaaaagca agctcaaaca actcactaaa
     1441 agaaaccgtg gaagaagtat ccaacttatt ttaaaagaaa tcaagcaact aatgactggt
     1501 tggatcaact actatggaat tggtgagatg aaagaattca tgagggagtt aaacggttgg
     1561 ttgaagcgaa gaatcagaca atatatttgg aaacaatgga agaatccaag aaccaaaaga
     1621 aaaaatctga ttcgtttag
//
LOCUS       DAYYKN010000253         1622 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000253 DAYYKN010000000
VERSION     DAYYKN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.261"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..279
                     /locus_tag="ACHBB7_002920"
     CDS             19..279
                     /locus_tag="ACHBB7_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210796.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(641..880)
                     /locus_tag="ACHBB7_002921"
     CDS             complement(641..880)
                     /locus_tag="ACHBB7_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210797.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1166..1480
                     /locus_tag="ACHBB7_002922"
     CDS             1166..1480
                     /locus_tag="ACHBB7_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210798.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          558 a          219 c          286 g          559 t
ORIGIN      
        1 attaaggatg gatgaaatat gaataacgaa actatcattt taggaaaaaa atatatttgc
       61 caacctattg gcttgaaaca aaaagtgatt ggagaagtaa tcagtaaatt agaaaattgt
      121 gttgtgatct gcgtagacga atatcatgac gtcgatcgca atgaaatatt agaaaaatgc
      181 ggaaaagtag tggcaaaata tactcacgtt tatgagttgg aaacaacaga atatctatat
      241 aactatagca acaaatatga acatacttta gtttcataaa gatttaaata aagtgccttc
      301 aaaagcgaga agaccaagct tttgaaggca ctttatttag ttggcagtga ttgttcatga
      361 tttttgattt attcaaaagg ttcgcctaga gaatctgtct ctgtaacgag gcctggtgga
      421 gaaactcgat aaaaacctat actgccagaa ctgtcctatt ttcggataga caaactagta
      481 gttttaaaaa gatcatccga tccaatcatt tcaaaaaatg caaaactgat atcaggatca
      541 tttagctcag gcttgttatc gtaaagcatt tgtaaaggtt gcatttagtt aggagtaaac
      601 gcgaaactat catcattatt tgctgaagaa gatgaagaag ctaattcaga gtttgtttga
      661 aataaaggag acgtgctgac agtttcttta ccctgttttt tgcagaggta gaaactttta
      721 tatcggaagt ttctaccgga gaatttttct catttgtaga aattgttgaa tcgatactat
      781 tcgaaatttg cgtatctgaa ggtatgttct ttgatgcaga agtcccacaa gcagtaagta
      841 acaataaatt tgataaactg ataaacagaa ttcttttcat tatgtacaca ctccttatag
      901 tatcgtttag catacaccta tgctcagtat taggcaaaat caatgcaaaa ctatcaagtc
      961 ttttatagta tctattagtc ttagtaatac atatatggag agggtgatat cccagttcaa
     1021 accagataca ttggttacca tctgtattaa cgattatgcc tctaaacgct ttttttatga
     1081 aaaaatttat aagtgaaaac gtaggtatga tattatctag aggtaatttt tttgggtatt
     1141 taaatagttg ctaggaggat agcttttgga tttttacagt aattttatct taataatagc
     1201 aatattatta cttctaaata tttggttttt tgataagagt agaaatgcag ggataggttt
     1261 tagaaccaaa cgaagtacgt cttctgaaaa aaagtgggta tattcacaga ctatttttta
     1321 cggaggggtt atttcaatca gtttactttc ttcaacactt tattctttta atgttataga
     1381 cgtttccatg tcaaatttta tatctattat tggaatcctt atttctgcaa ttattacaca
     1441 actacttctt gtgtttgaag agaagtcaaa aaataattaa agcaattatg ccttttagta
     1501 caggaattat tacttcttac aaatgtatag cgaatgtgaa gcctgctgtt ttaattgttc
     1561 tagaaaaact tttgagtgaa caattatttt atgttaggat gaattaattt ctctagagtt
     1621 ta
//
LOCUS       DAYYKN010000254         1501 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000254 DAYYKN010000000
VERSION     DAYYKN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.263"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1192
                     /locus_tag="ACHBB7_002923"
     CDS             <1..1192
                     /locus_tag="ACHBB7_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM9210799.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIV
                     VVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQ
                     YHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDN
                     KTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
     gene            1326..>1501
                     /locus_tag="ACHBB7_002924"
     CDS             1326..>1501
                     /locus_tag="ACHBB7_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9210800.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDL"
BASE COUNT          597 a          211 c          319 g          374 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct gatggtacga ttgataccaa cacaccaggt aattacgaag gggttatcga
      421 agtaacgtat ccagatggta cgaaagatac agtgaaagtt ccagtagaag tgacagacaa
      481 tcgttccgat gccgataaat acaatcctga aggacaaaaa gtgactacag acttaaataa
      541 agagccagac gcatctgagg gaattaagaa caaagaagat ctaccaaaag gaactactta
      601 tacttggaaa gagaaagtag atgttagtac agctggaaac aaaaaaggga ttgtagtagt
      661 aacatatcca gatggatcta aagaagaagt agaggttact atttctgtag aagataaaaa
      721 agcaccgaat aaacctcaag ttgatcctat tacagagggt gaccaaattg ttactggtaa
      781 aactgaacca aatgcagagg tgacagttac attaccaaac gggagtcaat accatgggac
      841 agctgataaa aatggtaatt ttacagttaa agttcctaaa ttagaggcag gaacaaaagt
      901 gatagtaact gcaactgatg aatctggtaa tactagtgaa cctactaatg tagttgtttc
      961 ttcaaacgaa aaagacagtg aaaaagccgt tagtaaagat aataagactg acaatcaagg
     1021 tagcaagcaa aacaaaaata gaggcaaaag cagtcctcaa aagcagtcct caaaagctta
     1081 tcctaagacg ggagagattg atagtaatat ctttactatt agtggtggtt taatcctgtt
     1141 aggaacttta gggttattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa
     1201 tttcttaaat cttctctgaa aagaggaaga atctttttct tcctcttttt gttttttatt
     1261 ttgggagata ggttgttagt taaaaatgaa gatacttttc atagaaggga aaagaatgtg
     1321 attaagtgaa aaaaatagta attattggat caaaccattc ggggctagct gcagctcgat
     1381 ttctaaactt atgtgattca aattatgaag ttattctgat agataaaaaa tcagaaatta
     1441 gttacttaga atgtgggaca tcgttgttca taaaaagaga agttagcaat ttagaagatc
     1501 t
//
LOCUS       DAYYKN010000255         1501 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000255 DAYYKN010000000
VERSION     DAYYKN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.264"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..125)
                     /locus_tag="ACHBB7_002925"
     CDS             complement(<1..125)
                     /locus_tag="ACHBB7_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFM9210801.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
     gene            complement(143..463)
                     /locus_tag="ACHBB7_002926"
     CDS             complement(143..463)
                     /locus_tag="ACHBB7_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9210802.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            762..>1501
                     /locus_tag="ACHBB7_002927"
     CDS             762..>1501
                     /locus_tag="ACHBB7_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM9210803.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEG"
BASE COUNT          557 a          258 c          234 g          452 t
ORIGIN      
        1 ccgtatagat tttcattcaa aaaaatgatt ctgggttttg gatagtagaa accaggctct
       61 ttcatctcca tataaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
      121 tacatacaaa ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg
      181 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtctt
      241 ctgataaatc cacatgttta ggggtttctc tttcttctct acctagaagg tagtctacag
      301 agacatcgaa atagtcagca atccgactta attcatctga attaggagta ttatttttcc
      361 actttgctag ataaccgtta gaatatccaa aattaatttc taactgccgt atagatatct
      421 ttttcctctt cgccaagtct tttattattt cataggtatt catcgataaa acaacctttc
      481 taaatgttta caaaaaaagt ttagaaaaat acgcagaaat aatttgacta attctgagta
      541 atgagctata ctatatcttg taaacagatt taacaactaa aaagacaaca aaaaacacta
      601 ttgatatgta aatgcagacc gccaagaaag ctttaaaatc aatgttttta tgtcttattt
      661 aattatgctc ttagtataga gtattactca gatactgtca attgaattta gaaaaaagtt
      721 gttaaatttg tttacgaata taaaagaaag gaatgagaaa tatgaacaca ccgcaaattt
      781 ttaatttcga acaaaacgaa gttcggacaa ttttagtaaa tgatgaacca tattttgtag
      841 gcaaagacgt tgcgagtgtt ttgggttatt caaacactaa agatgctttg tcgcggcatg
      901 tagatttgga agataagatg gggtcgcgaa tcacgacctc aggtcaatca agagagatga
      961 caatcatcaa cgaatctggt ttgtacagtt taatcttaaa atcaaaactt ccctctgcca
     1021 aaaaatttaa acgttgggta acaagtgaag tgttgccagc aattagaaaa catggaggtt
     1081 atctaactcc agaaaaagta gaagaagctt tgcttaatcc agatacaatc attcaattag
     1141 caactcaact aaaagaagaa agaactggaa gattaatcgc agaacaaaag attgcagagt
     1201 acgaacctaa aatctcctat ttagatagca tattatcttc tacagattca gtaacaatta
     1261 gtcagattgc agcagattat gggatgtctc cacaacagat gaataaatta cttcataaac
     1321 taggtattca gaaaaaagtc ggtaaccaat ggttattgtg caaaaaacac atgaaccaag
     1381 gatacacaaa atctcataca actgagatcc cgaaagccga tggtggcact aaaattgtaa
     1441 tgaataccaa atggacacag aaagggcgtc tatttatcta cgaattacta aaaaaagaag
     1501 g
//
LOCUS       DAYYKN010000256         1474 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000256 DAYYKN010000000
VERSION     DAYYKN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.265"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..>1474
                     /locus_tag="ACHBB7_002928"
     CDS             119..>1474
                     /locus_tag="ACHBB7_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210804.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CL"
BASE COUNT          528 a          203 c          272 g          471 t
ORIGIN      
        1 atgagaataa ctgatgaaaa aacaagcata atccatcata aaatcagtat aggtgaaaag
       61 atgaactttc atctgcatat caagtaattg gtatgaggca ttttttgagg tgtaggatat
      121 gaagttggat taccttatag aagaggaaga tagagaaaaa ctgctgctag ctagatattt
      181 agaatttgag aaaaagaatt atgtagtgct gcctaagtta tatgaaacat taggtttgtc
      241 taaatttaaa acaagaaatt atatcataga attaaataaa gaatttactg aattcaaaac
      301 aaagccagag atccaaattt cagaagaagc agagatccat gtcgaacgtc tgaatttgac
      361 ggatatcaaa aatttaagaa gaatctactt tcaacgatca aaaattgctc gactgttaac
      421 ggagatcgtg gattcaaaag taacgatcga aaaatttgca gagaatcatt ttttaagcat
      481 atcaagagcc tatgtaaaaa gaagagaatt agtctctttt cttgaacagc atcaaataaa
      541 gttaaagaaa aacatattag ttggggaaga aataaacata cggaacgtat tatttggggt
      601 atatttcggg gtctttaatg gtttttctat gccatttaga gaaagcgtca tgaatgaagc
      661 aaagagtatc gtgcaatatt ttgaatatct gttcaattta aaactgtctg caacaaaacg
      721 aatcaaattc aatttgctaa ccactattag tttgttgagg gcctatggga atcaaccaat
      781 cggaaaacgt ttcttttcag atgaatactt taagagctca gaaggaatga agatcatcaa
      841 ggatttatca catatttctt ttcttcatat ccctaaagag cagatcattg atgagatttc
      901 ttatatcatg ttgtttttga tcacggaaga agaatttgaa ttagatggcg catttggact
      961 tgcggaagct cattttcatg atatccacat gattagtcaa aaaatgtcag tggaattgat
     1021 tgatcaatta ccattttcga ttgaaatctc tgaagaagaa agactatata tacgaaaaga
     1081 acttcgtatg ggtatgatgc atgtcaatcg aaaaaattgg atattcgact ttgagatgac
     1141 tagctttacg tctaaaaagc agctacagtt tttcttcgag acgtatccaa tattcagtac
     1201 agcaatccaa aaggtcatac aagagaataa acgctatttc ttgggaaaag atgaacgttt
     1261 catgattcgc ttattttatg attatctctt tttattggtc ggtatttgtc cggtatcata
     1321 ttttgaagca ccgattcatg tctgtattga tttttcgcaa ggatctgctt ataccgatta
     1381 cattatttct caagtgaaag gatttaaaaa ttacaatatt gtgattgaga gtcgaatcac
     1441 ttctcgtacg aatatttatt tatctgattg ttta
//
LOCUS       DAYYKN010000257         1374 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000257 DAYYKN010000000
VERSION     DAYYKN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.266"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..1372
                     /locus_tag="ACHBB7_002929"
     CDS             join(33..474,474..1372)
                     /locus_tag="ACHBB7_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM9210805.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DAYYKN010000258         1373 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000258 DAYYKN010000000
VERSION     DAYYKN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.267"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..1339
                     /locus_tag="ACHBB7_002930"
     CDS             38..1339
                     /locus_tag="ACHBB7_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9210806.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          447 a          275 c          253 g          398 t
ORIGIN      
        1 attgaatcac cacaacacaa tcaaaggagc gttctgtatg cctgatcttc atcatatcaa
       61 actactactt ggaatcaaag ataaaaacat tttcatcaca aatgtggaat cgaagagcat
      121 taagaatatc aagagtcttg ttgtttctgc gacgttaagt aaagaaattc gtcgctgtcc
      181 tttgtgtaaa cagatgaatc atgaaggcat gatcgttaag aacggaaaga aaaaatcgct
      241 cattcaactt aataaatgtg cgaaccaact gacttatctt gctttagcga aacaacgcta
      301 ccactgtcgt ggctgccaca cctattttac ggcgaatacc tacattgtcg atagaaactg
      361 ctttatcgct aaacaagtgc gctataaaat tttagaagag ctaactgaaa aacaagcaat
      421 gacgacaatt gctaagcatt gcggcgtttc ttggtcgacc gtttctcgga cactcgcttc
      481 tctgatcccc atgacaaaag taaaaagaaa ttggttaccg cgttgtttat taatggatga
      541 atttcgttca ctgaaaaatc aagtcggtcc ctactccttt tcttgtatgg atggcgatac
      601 tggaaaatta ctggatattc ttccttcacg gaagaaaaag gctctcgttt cttattttat
      661 gcaatttgaa cgacgcgcac gattgaaggt aaaggtcatc gtgacggaca tgaatgtttc
      721 ttatgcctct ctgataaagg aatgttttcc aaaggcaaaa ctcgttgtgg atcgttttca
      781 cattgtcaaa catctcattc gtaaatttga ggatattcgc gtacgaatca tgaaaagctt
      841 tgatcgaaac gatccaacac aagccaaata ttatcgacaa ttaaaggcgt tgtctcggct
      901 tctgatcaaa cgacaggaca agcttgttta tgacaaatgg accaaatggc gcaattttgg
      961 ttgggcgtat ctcaccgaaa gtgaagtggt ggatcgtctg ctttcgatct ctgacgaatt
     1021 acgaatcgcc tatttttact atcaagaaat tcttcaagcg tttcacgaca aagaggcgga
     1081 cactttcttt aaacttgtaa gaacgatgcc caattccgtt ccaaaagaac tgcattctat
     1141 aaaaaaagct tttataaact atgaatccgg cattcgttta gccctcgaat tgccttattc
     1201 aaatgccaaa atagaaaatc tgcataccca catcaaagca ttgaaacgag tcgcttatgg
     1261 ctttcgaagc tttcggaaaa tgaagaccag aattttctta ctaaataatc tgattaccta
     1321 tgaatcaaaa aacatctaaa gtagaaaagg aattctacct tagatgttct tat
//
LOCUS       DAYYKN010000259         1357 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000259 DAYYKN010000000
VERSION     DAYYKN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.268"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..1035)
                     /locus_tag="ACHBB7_002931"
     CDS             complement(112..1035)
                     /locus_tag="ACHBB7_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210807.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLENINETVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT          446 a          244 c          135 g          532 t
ORIGIN      
        1 gatcttcagt tctacaaaaa tatatcccac tcaaaatctt gacaaaagaa aaaagttgtg
       61 atttccaaaa tcacaacttt tttcttttac aataaatcaa tccttattaa attatttagg
      121 aaaatattgt tcaattattt ctttatatat tttctcatag tatgtttctg tccatcctaa
      181 tgttcctata aaatcaagtc ctctacattt taaaggttca cttaaactat taatatagtt
      241 aataatgttg tctatccttt tctctttatc ttcaccttca atctggggag catatagtcc
      301 aatttcctcc caataatatg aatctctttc aattcccatg atggtagctt tggctaattt
      361 caaatcaaat aacgatttta gaattcttag tgtctcaata aagtcttctc tcttcaactc
      421 tttaatccat tgtgaatttt ctggattatc taaaatttca agttcatctt cattataagt
      481 atatttcaaa tttgacttgc ttaactcata gataaatgaa gtttcttgac tcataattct
      541 tttaagtact tgactataag tttcgtttga ctttgcccat ttcgctaatt ctaccactgt
      601 attcactaat aaatcgaaat aatgatgttc tcgttcaata gtctcagcat tttgaaaatg
      661 aaaatcacct ttataaactc cattcttttc cactgtttca ttaatatttt ctaaaaacca
      721 gaatgagttt gcaaatttag tcgtactttt tcgattaact aagttaataa ctcttccatg
      781 taataataag acaaaaatac ataaattttt agcttcctta ttattagata ctaattgatc
      841 aatcataggg agccattttt tgattttcac taattgattg ctcataaatc tttcactgga
      901 cttttgttca atcttaaatt gcaaaacttc taattcataa tttccatctt ctgatttatt
      961 tttgggaaca actatcaaac ttttataaag tttatccaca tcaaaattta tcttttttgc
     1021 gtaatttgta gccattaata catctcctta ttcataataa atttcatatg acgctcctct
     1081 caaaatttac cggtagacta gaaaaaagaa taaaagatac gtcaattcac actttattct
     1141 taaaaaaaac tggtctctct accggtaata tcgattgagt ccgcctttat cgactttatc
     1201 ctttttttat attaaatgtt ttcaccggta gacagggaga gaaagaataa actaaaagcc
     1261 cttaattctt aatgttttca cctccttacc gttcctaccg ctaaaattta atataactaa
     1321 attctataac attagaagaa attttaaata cctttga
//
LOCUS       DAYYKN010000260         1357 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000260 DAYYKN010000000
VERSION     DAYYKN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.269"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..437)
                     /locus_tag="ACHBB7_002932"
     CDS             complement(<1..437)
                     /locus_tag="ACHBB7_002932"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210808.1"
                     /translation="MFDKNINSEIGKRIQEIRVSLGYTLEEFGENLTPPASKSNVSRW
                     EKGKNIPNKARLLSIAKLGGVSTEYLLTGNSQKTKKYIKYLFNEIFFDNGTFHNKFKD
                     FGKDRDRLGLYDLFLAVSKTYSLDKSPSLKDNFVEMIIDDVYK"
     gene            601..807
                     /locus_tag="ACHBB7_002933"
     CDS             601..807
                     /locus_tag="ACHBB7_002933"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9210809.1"
                     /translation="MKPKDFRKFCGLSQSELAEKYGISVQAVRNKESGKTNYSPEEMI
                     ILKYLVKEKGIENITIDQIFFNEK"
     gene            858..1139
                     /locus_tag="ACHBB7_002934"
     CDS             858..1139
                     /locus_tag="ACHBB7_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210810.1"
                     /translation="MKSEIQVILPSDLDQELRRYFYRVAKEAVDQVRIETSLSKEVFR
                     LGEACKYLSCSRSTLKKFIREKGLKVSIIGDCQFIRKEELARFIKEYEV"
BASE COUNT          508 a          169 c          245 g          435 t
ORIGIN      
        1 tttttatata catcatctat aatcatttct acaaagttat cttttaaaga aggtgattta
       61 tctagactat atgttttcga tactgctaaa aataagtcat atagaccaag cctatctctg
      121 tctttaccga aatctttgaa tttgttatgg aaggtaccgt tgtcaaaaaa aatctcattg
      181 aataaatact tgatatattt cttagttttt tgagaattac ctgtcagtag atattcagta
      241 gaaacaccac caagtttagc aatacttagt agccgcgctt tgttgggtat gttctttcct
      301 ttttcccatc tagaaacatt gcttttgctt gcgggaggtg tcagattttc gccaaattct
      361 tcaagagtat accctaaaga aacacgtatt tcttgtattc gttttccaat ttcagaatta
      421 atgtttttat caaacaaaat ggtttcacca ccttcatcta ttctattcta cagtttacac
      481 tacattctca tataatgaaa tgaaaaagta gataaaagtt gaattttacg ttgcggtatg
      541 aataaatata tgttattctt ttagtgacga aaagttgaaa agaaggagga gtgaggagtc
      601 atgaagccta aagattttag aaagttttgt ggattatcac aatctgaact tgctgaaaaa
      661 tatggcattt cagtacaagc ggtaagaaac aaagaaagcg gtaaaacaaa ctattctcca
      721 gaagaaatga ttattttgaa gtatcttgtg aaagaaaaag gaattgaaaa tatcacaatt
      781 gatcaaattt tttttaatga aaagtgacga aaagttgaat ttgaatacat gtagtcaaat
      841 tagtgggggc aaagaaaatg aaatctgaga ttcaagttat tcttccatcg gatttagatc
      901 aagaattgcg aagatatttc tatcgagttg caaaagaagc agtagatcaa gttcgaattg
      961 aaacatctct aagcaaagaa gtttttagat tgggggaagc ttgtaagtat ttaagctgta
     1021 gcagaagtac attaaaaaaa tttatcagag agaaaggttt aaaagtaagc ataatcggag
     1081 attgtcaatt tattagaaaa gaagaattag caaggtttat caaagaatat gaagtataaa
     1141 aagttgcgtg gcgtgctttt gatagaaaaa aggaagtgtt tgtttgaata atgatgaagt
     1201 atagaaaata taaaaatact ttgaaaaaaa ttaaaaggag tgttcccgac agtttggcga
     1261 ccacaggaaa cactcgacaa taaagttcta agaaaacttt atttgactac attttaacat
     1321 ttattagaaa gaagaggaat aaatggaaga aatagat
//
LOCUS       DAYYKN010000261         1335 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000261 DAYYKN010000000
VERSION     DAYYKN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.270"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(316..1206)
                     /locus_tag="ACHBB7_002935"
     CDS             complement(316..1206)
                     /locus_tag="ACHBB7_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM9210811.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          340 a          287 c          241 g          467 t
ORIGIN      
        1 tacaacaagg tctgatcttt gaaaactaaa aaaatatgcg gcgcacagaa cgcgccgcat
       61 atcggtctaa gttttattca aatttgtctt caccaacacg atttgacgaa gagccaaaaa
      121 aagttacgac agttctatgt cgtagctctt tttttcgaat aaacggtgtt caatcagctg
      181 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctatttttca caattaaagg
      241 aaaaatgtat atgtggggaa acatagttga tttttcctta atagtaacga ctaccgtggc
      301 atgtttatct gctagttatt tgatatttcc ttcgtgatca cgttctactt ccatcattgt
      361 gattggtaaa tagcccaatt ctttgacagg tccttcttgg atttcttctg tcatcatgta
      421 gtctaagaat ttttggactt caggggaagg ttttccgttc gtgtacatat gttcatatga
      481 ccagattccc cagctgttat ctgctacatt ctcttctttt ggttctacac cgtcgatact
      541 tagtgcttgt gtggaatcat cgagatatga gaaagctaaa tagctgattg ctcctggtgt
      601 ttgacttacc agctgccgaa ctgttcctga tgaatcttgt tcttgggact gcacaggggt
      661 agcaccatcc agtccccatt tttcgaatgt cgcgcgggta ccgctgccgt ttgcacggtt
      721 cacgacgttg attttttgat ctttcccgcc aacttccttc cagttagtga tttttcctgt
      781 aaagatatca atcaattcct gtttggtgat atctttgaca tctgtatctt tattgacgat
      841 tggtgccatc ccgaccactg ctactttatg atccactaat tttgaagcat caactccgtc
      901 tctttcttct gcaaatacat cagagtttcc gatagtaact gctccagctt cgacttggct
      961 caaaccagtt ccgcttcctc ctccttgtac agagatttga taatttggat tttcttgtac
     1021 aaagctttct tgtgctgcat cgactaacgg ctgtaatgct gtcgaaccta ccgcaacgat
     1081 ttttacttgt tcatttgagt cacttgaatt attttgtgtg gcagagccat ctgtatttcc
     1141 tgatcctgag ccgcatccgg ctaaaagaat tcccatcata cctagagtaa acaatacttt
     1201 tttcatttta ttgctccttt cttatttaca actaaagttt atcagtcgac tgtaaatccc
     1261 tcgtatggtt gctgtaaaaa tatagttaag ttatgtaaaa atcatgtaaa cgtttttcgc
     1321 attctttaca taata
//
LOCUS       DAYYKN010000262         1311 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000262 DAYYKN010000000
VERSION     DAYYKN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.271"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACHBB7_002936"
     CDS             200..742
                     /locus_tag="ACHBB7_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210812.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1311
                     /locus_tag="ACHBB7_002937"
     CDS             898..>1311
                     /locus_tag="ACHBB7_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM9210813.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMA"
BASE COUNT          532 a          148 c          255 g          376 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DAYYKN010000263         1281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000263 DAYYKN010000000
VERSION     DAYYKN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.272"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..427
                     /locus_tag="ACHBB7_002938"
     CDS             32..427
                     /locus_tag="ACHBB7_002938"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9210814.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            424..>1281
                     /locus_tag="ACHBB7_002939"
     CDS             424..>1281
                     /locus_tag="ACHBB7_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9210815.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     KYLNIKKDII"
BASE COUNT          512 a          143 c          243 g          383 t
ORIGIN      
        1 aacaattaaa tcattattta gttaaaaaga tatgttttgc aaagtatgtg atagaaaaga
       61 gggattctgt ggcttcagaa tagatatcaa agaattggca aaagtttctg atattttttt
      121 atttactaca ttacaagaag gaatgccaag atctatgatg gaagctatgg caagtggatt
      181 gccatgtata gcaagtaaaa tccgaggtaa tgtagacctt ttagtggaag gaaaaggtgg
      241 atatcttgtt ccagctaaag atgcagaatt agtagcagag tgtttgagag aactaatcaa
      301 taataaatct cttagaaagt atatgggaaa tgagaacctt tccagaatta aagaattcga
      361 ggttagtatc gtaaaaaaaa gaatcaaaga aatatatgaa gaagttatag gtgagagtgt
      421 aaaatgaaaa atccaatgtt ttcaataata gtacctatct attgcgttga aaaatatttg
      481 aaagattgct tagattcaat aagaaatcag acatttaaaa attttgaagc aatactagtt
      541 gatgacggta gtcctgacga atgcccagaa atatgtgact attattccca aatagatgga
      601 cgtttaaaag taattcataa gaaaaacggt ggaataatag atgcacggaa aacaggagtc
      661 actagtgcta tgggagagta tatagttttt ctagatggtg atgattggct tgcaaatact
      721 tatttagaaa aatatgcgga agtaattact aagtacaatc cagatataat atgtagtggg
      781 tattttgcat cacgttccaa caaaattaga aaaagagaat tgtcttgctc gtttggatat
      841 tatgacaaag ataaaattat aaatttgatt tatcctaaat taattgagga tgaacatgga
      901 gagtatttta gaccacaatt atgggcaaaa gcttttagaa ctactctgta caaggaacaa
      961 caacaagtat ctgggcaagt tagtgttggt gaagatcatg catgtacaaa accatgtatt
     1021 taccatgcaa attcgatata tatgatggaa aaatgtttat attattacag aataaacgaa
     1081 aattctataa caaaaaaacc acaaccatat tattgggaag gaccaaaggc tattggagag
     1141 cattttgaaa agcagattga tatgaataag tatgattttc aagatcaagt atatagaaat
     1201 gttacacaca atttatttaa tgttgctgtt tcacaattta ataaacagga caaatattta
     1261 aatataaaaa aagatattat a
//
LOCUS       DAYYKN010000264         1276 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000264 DAYYKN010000000
VERSION     DAYYKN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.273"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..532
                     /locus_tag="ACHBB7_002940"
     CDS             35..532
                     /locus_tag="ACHBB7_002940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9210816.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="ACHBB7_002941"
     CDS             complement(569..1252)
                     /locus_tag="ACHBB7_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM9210817.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DAYYKN010000265         1251 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000265 DAYYKN010000000
VERSION     DAYYKN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.274"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..713
                     /locus_tag="ACHBB7_002942"
     CDS             42..713
                     /locus_tag="ACHBB7_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9210818.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(873..1064)
                     /locus_tag="ACHBB7_002943"
                     /pseudo
     CDS             complement(873..1064)
                     /locus_tag="ACHBB7_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          455 a          192 c          174 g          430 t
ORIGIN      
        1 attaaaatat cagttaaaat aaaagttaag gagcattgtg tatgacaatt aaaactagtc
       61 tccaaaatca agcttaccaa tctattcgcc gacaaattat ctatgctgac ttagaacctg
      121 gaaaaaaagt atcagaaaaa aaattagaag aaacactttc tattggtcgt acaccaatac
      181 gtgaagcact gattcaactc cgacaacaag gtcttgttga tacaattcct caaagcggaa
      241 attatatctc attaattgat ttagattcgg ccaaaaatgc tcgatttgtc agagaacatt
      301 tggaacgaca aattatgtta gagtgttgtg ccaaaatgaa taaccaaaaa aaacggattt
      361 tagaaactat aatttcagaa caagaaaaag ctgttagtca aaaaaatgct cgtagttttt
      421 ttcataatga taatttattc cataaagctt gttttgaaat tgcagagcga gaagaagtat
      481 ggcaatggtt agaaaataac aatacacact tagaacgatt tcgatggcta cgagtaaaaa
      541 cggaaggatt aaagtgggaa aaaattatgg aacaacatta tcaactattt gaggcattaa
      601 caaaacaaga tccagaagaa gctaactttc taacatcgtt gcatcttcat ttaatgctta
      661 atgagcaaaa tattgtttta gctactcatc caaaatattt caaaaatata taacttttaa
      721 ctctttttaa attaaatggt tgttctgatc agaaaaatat cgcttgtttt cttattatgt
      781 aatatatttc ataaactatt ctgcataaca actattgttt gtacttttta cattggataa
      841 ctaattatga ctgctgataa cttttgatat taaactagta tctccaatac aaataaaaaa
      901 tagatatctt gttcatcaaa attagttcgt ggatacttac cttcatttac atcattcagc
      961 ggtttcccat tttcttttat tctattattt cattttctaa tagacttggc ttcatttcgc
     1021 aatacttttt ttcaataggc atcaaattgg tgttgtttag atatttagtt gtagttttgt
     1081 gacatgataa gtatgctcct ttttctcttt tccactaata caacgatcaa agggtgcttt
     1141 tttcaaaaaa ctagaaaaat caaaataaga ttcgtatttt gattctatat atcctgtttc
     1201 aactacatct tatatttcac aatagaataa atagcctttt gcatagctgc t
//
LOCUS       DAYYKN010000266         1248 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000266 DAYYKN010000000
VERSION     DAYYKN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.275"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..900
                     /locus_tag="ACHBB7_002944"
     CDS             <1..900
                     /locus_tag="ACHBB7_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9210819.1"
                     /translation="NQSVAKTAHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSN
                     CGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDL
                     PMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITL
                     VERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVN
                     DIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKS
                     LDYRTPLEVFLSYVSIDDLSNLI"
     gene            1033..1239
                     /locus_tag="ACHBB7_002945"
     CDS             1033..1239
                     /locus_tag="ACHBB7_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM9210820.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          431 a          197 c          253 g          367 t
ORIGIN      
        1 aatcaatctg ttgctaaaac tgcccattgc ttgaatcgtt caagacaaac gatccataaa
       61 gtatatctgt tcttcaagca aggaaaatca gccttagaat attatcaaca gtataagaaa
      121 aacaaatcaa actgtggtag acgtccgctt gttttacccg aggaacaatc agaatatatt
      181 caaagaaagg ttgttcaagg atggacaccc gatgtgattg ttggtcgtgc agcgtttcct
      241 attagttgtt ctgctcgtac catttatcgt atgttcaaaa aggggctatt tgattcttct
      301 gacttaccga tgaaaggcaa gcgtaaaccg aatggacatc aagaaagacg tggaaaacaa
      361 actttccgcc gctctattca tgaacgtgaa aaggattata gccaattctc aaatgagttt
      421 ggtcaccttg aaggtgacac tatcgtaggt ctgaaacata aaagtgctgt aattacctta
      481 gttgaacgat tatcaaaagt tatcatcaca ttgaaaccgt gtggtagaca agcgattgat
      541 attgaaaaaa aattaaatca atggtttgaa tctgtaccga aaaacctatt caaatccatc
      601 acttttgatt gtggaaagga attttcaaat tggaaacaga tcagtaatgt caatgatatt
      661 gccatttatt tcgctgatcc aggaacgccg tctcaaagag gcctaaacga gaattctaac
      721 ggattgttac gtagagatgg tttattgaaa tctatggatt tcaattcagt agatgaattt
      781 tttattcaat ctgtcgcatc taaacgaaat aatattccta gaaaatcact ggattatcga
      841 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
      901 caattaagaa agataaaatt gaacaactgg acgggcaaat tgatgccttg ctttcagaaa
      961 gagaaatatt gatagaagaa aacaagcgat tgaaaaagaa agtagcccaa ccaatgaagg
     1021 gaggtggata gtatgaacaa tagaaccttt gatattttga aatggatcgc tatagtagtt
     1081 atccccgctt tagcgacttt tgtcggtctt gtcggaaaag gtgttaactg gcggtacaca
     1141 gatataacgg tgatgattat tacaggtttt ggagcatttc tagggagtat tttaggtgta
     1201 tcaaatcgaa cgtataagaa aatttcatca gataaatagg aggataga
//
LOCUS       DAYYKN010000267         1215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000267 DAYYKN010000000
VERSION     DAYYKN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.276"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACHBB7_002946"
     CDS             414..947
                     /locus_tag="ACHBB7_002946"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM9210821.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAYYKN010000268         1193 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000268 DAYYKN010000000
VERSION     DAYYKN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.277"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1026
                     /locus_tag="ACHBB7_002947"
     CDS             <1..1026
                     /locus_tag="ACHBB7_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM9210822.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
     gene            complement(1080..>1193)
                     /locus_tag="ACHBB7_002948"
     CDS             complement(1080..>1193)
                     /locus_tag="ACHBB7_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9210823.1"
                     /translation="FISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT          423 a          176 c          202 g          392 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaa
//
LOCUS       DAYYKN010000269         1168 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000269 DAYYKN010000000
VERSION     DAYYKN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.278"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1155)
                     /locus_tag="ACHBB7_002949"
     CDS             complement(1..1155)
                     /locus_tag="ACHBB7_002949"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM9210824.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT          320 a          255 c          219 g          374 t
ORIGIN      
        1 ttatattttg tagtaacgaa tatcattttc gatctttgta atcgaaaaag gtacatctga
       61 agtatattct gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg tttcatattc
      121 gtcaacagtg atccgtgttc gagaatctag catctcttga tgagattgtg cgaagagata
      181 ttcttgatag ttttcttcca gataacctgt aaaaaattca cttactgcac cagaaccgta
      241 gctgaataga ccgatgcgat ctcctggctg tagtgatttc gaattttcca gtagagaagt
      301 tagccccagg tataatgaac cagtgtaaag attaccgatt cgtcggctgt aacggatgct
      361 ttcttcatag cgagccataa gacgttcctg attttcttcg tctgtttggt ctaatacgct
      421 ttgcaatgcc ttttttccca tcttagtata cggaatatgg aaagcaatcg cctgataatc
      481 ttcgagtcct cgacccgaca attctttatg tcgattccaa actttttgga atgattcgat
      541 atacgtagaa ttagataaag gaccatcaac aacaggaaat tcgctataat ctggacgcca
      601 gaaatcatag atatcttctg tcagaaatac gctgtcgtct tcaatcgata aaatacgcgg
      661 gttttgagtg atcatcatcg caacagcacc gacaccttgc gtcacttcac caccgcttgc
      721 caagctgtaa cgagcaatat cacttgctat gactagtact tttcgttctg gatgattttt
      781 gacatattct ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct cttttacttc
      841 aaaagaacga gcaaaagatt ggattttcag taaatggtgg ataattaccg cactcgcttt
      901 tgaatgatca atcccggatt ctgttccgac gatgaccatg tcgattagct cacggtcttt
      961 ttctgttacg attttgctgg cagcatttgc tcctagtgta atgatgtctt catttgcacg
     1021 attgactgcc atttggtctt gaccaatccc tatatggtat tttgcaggat catccccacg
     1081 gctttctgcc aactctgtca tatctaaata taaattagga ataaaaaagg aaagacgatc
     1141 aatccctatt ttcatttcaa cacctctt
//
LOCUS       DAYYKN010000270         1156 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000270 DAYYKN010000000
VERSION     DAYYKN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.279"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1061)
                     /locus_tag="ACHBB7_002950"
     CDS             complement(<1..1061)
                     /locus_tag="ACHBB7_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9210825.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITE"
BASE COUNT          304 a          257 c          183 g          412 t
ORIGIN      
        1 tcttcggtta ttctttcttc ccaggatttt ggtcttaagg cctcattcag attgataaac
       61 agctttttta ctcgtttctg atactccttc atgacctttt tcattaatgt tttgattcta
      121 cgactataga ttccatagcg acgaatcgtt ttgaaattct tattttgaat atggcgaatc
      181 agtgcagcta tgaattcgtc tatactcatg gtcatcgtct ctttagtatt cgttcgtttg
      241 tccatgtagt tgaacatgac agtgtcgcca tcatacatta agattcggct taatgcgatc
      301 gggccacgct tcatataacg actgatgtat tggagtagtg actttagatt tgtcctgctc
      361 cttttaggag catttacgta aaacccttct ccattgttct tgtacgcagc ttgtaatctt
      421 gattgaactc tttgcttgtc ccactcacct aacgttcgtc taattagttt caatacagca
      481 tttttccagt attttcttaa catagaaaaa ggtaaataat cgtattcttc ccaacttcca
      541 tctcttgtta agccacccat agtaacaacc atatgaacat gcggattaaa ttctagtttt
      601 gatccaaagg tgtggattgt agctactata cctgccttca cctttctttt ctcaaaatag
      661 gcaagaataa tttttgcggc ttcatccatt agtcctttca gtaaggaatc tcgatagttt
      721 tccatcataa agatttcccg taacccctca tcgatcgtaa agattacgtg ccgatgtgtc
      781 actttaaaca tatcattcgc cacaaccgct gaccaccttt ggctttcacc tgttgcacac
      841 gttggacaga actttccacg acatttcaag ggaacaaact ttgtttgatg acatccctcg
      901 cagacaaaca gtcggtatcc tttttgaata tctccacaat atctaaactt gtttacttcc
      961 cgtttgacga ctagccgaat ctttcgcccg tatttctcaa caaagttatc ccaattatgg
     1021 tgttcgtcaa aaaagattgc ttcgagaata ttcgctttca tagtaatatt ttacttgttc
     1081 aaaaacaaaa tgaaaagggc taaaagactg gtagtgcagc ctttttagcc cttaactttt
     1141 tatactttct ttactg
//
LOCUS       DAYYKN010000271         1149 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000271 DAYYKN010000000
VERSION     DAYYKN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.280"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..308)
                     /locus_tag="ACHBB7_002951"
     CDS             complement(<1..308)
                     /locus_tag="ACHBB7_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9210826.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     "
     gene            complement(438..>1149)
                     /locus_tag="ACHBB7_002952"
     CDS             complement(438..>1149)
                     /locus_tag="ACHBB7_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM9210827.1"
                     /translation="YENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKD
                     LGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMN
                     ARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVT
                     GDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLII
                     SSFNILRRNPKELLIDNN"
BASE COUNT          375 a          206 c          159 g          409 t
ORIGIN      
        1 ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc aatgtttact
       61 ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt gtcatgtttc
      121 tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc ttgaagcatt
      181 ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc tacagattcg
      241 ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt tcttttcttt
      301 ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc gtgaagcaac
      361 cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat ttttggtgct
      421 aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt ccttcttaat
      481 atattgaatg aagaaataat caatgataca agaagtacta aactcataaa tataactaca
      541 taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt gttaaatcct
      601 tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat ttgtttagct
      661 atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt atattgagct
      721 aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat taggaattga
      781 ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc tttctttcta
      841 gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat taatgagcca
      901 aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa tgcagggtaa
      961 tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt cccaagatct
     1021 tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc ttggtataca
     1081 gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt gattaatgta
     1141 ttttcgtat
//
LOCUS       DAYYKN010000272         1147 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000272 DAYYKN010000000
VERSION     DAYYKN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.281"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..138)
                     /locus_tag="ACHBB7_002953"
     CDS             complement(<1..138)
                     /locus_tag="ACHBB7_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9210828.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FK"
     gene            341..>1147
                     /locus_tag="ACHBB7_002954"
     CDS             341..>1147
                     /locus_tag="ACHBB7_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFM9210829.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKE"
BASE COUNT          467 a          133 c          192 g          355 t
ORIGIN      
        1 cttgaagaac agatatactt tatggatcgt ttgtcttgaa cgattcaagc aatgggcagt
       61 tttagcaaca gattgattaa ttttgaaata agactctatt attacaagtt catccgttgt
      121 aagatgggta taggtcattt gtactcactc cttataattc tttggtcgga actattttga
      181 gtgtagcaca aatgactttt ttagttgtct agcttaattt tacaatcggc gtgatgaaat
      241 aaatcaaaag tatgatgaaa cattaaaaaa tgtctattct tcacttatcg tttcaaatat
      301 agaagaaact tcatcttctg aaataaaaaa taagctttct ttgttaaaat taaaagaaaa
      361 ggaatatatt attgacgatg ctgtttctgt ccctcttatt ttaaataaac gagagaaaaa
      421 taatttaaag aaaaaaatgc tcattccatt tgattcagaa gtgaaaggat tactcaataa
      481 actaactatt tctaatatag actcgactag ggaaaaaatc ataaaaatat ttgataaaat
      541 taataattta ttcaagaatg acaatgctga attaaggaag ggtcttttag agtttaaact
      601 tcaagaacta gaacttcatt actcttattt agtgaaaatt accgaagaaa aagaacaaca
      661 aaaagcaata aaagaacaga tgatagaaga ggaaaaagtt cgtagagaaa ttgatcgtga
      721 aaagaaacgt attgataaag aacaacgtca attcaattct gaaatttcca aattaatcgc
      781 atatatgcaa aaagctaatg cagatgtgga aaaagaatta tatgcaaata aaattcaaga
      841 actggaagaa aaattaaaag aattagaagt tatacgtgaa aatgtattac aaagagaatt
      901 aaatactaga gccggttatg tgtatgtaat tagtaatatt gggtcttttg gtgaagacat
      961 atataaaatt ggtatgacta gaagattaga acctatggat agaataaaag aattgagtag
     1021 tgcatcggtt ccatttgaat ttgacgttca tgcgatgatt ttttcagagg acgctccgtc
     1081 tttggaaact aaattgcata atcattttag aaaacaagaa gttaataaaa ttaatcaaag
     1141 aaaagaa
//
LOCUS       DAYYKN010000273         1128 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000273 DAYYKN010000000
VERSION     DAYYKN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.282"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>1128)
                     /locus_tag="ACHBB7_002955"
     CDS             complement(37..>1128)
                     /locus_tag="ACHBB7_002955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9210830.1"
                     /translation="IQNKKIICVKKDGTRFSQEYDKLILATGSKQIMLDVPGNNLANI
                     FSIKNFPNAKEIFKALDDNTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVADRVLS
                     TYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIG
                     FLSNSPIGKKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVDFSISN
                     ALRTAYIATQHIDNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSESIDYIEIE
                     DFIRPVFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQITLDKL
                     KMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT          368 a          184 c          139 g          437 t
ORIGIN      
        1 taatacagta tatttcttga tttcatttag taacgattat gtttcaatgt ttaatcctgt
       61 tactgccttt aaaataaaat tatagggttg gctaaaacta ggatgaaata aataatctaa
      121 cattttcaat ttgtctaatg taatttgttc ttctatagca agagaaaaca ttgataatag
      181 tccagataaa tcttcttgac tacaaagttg tacacctact attctctttg tatttttttc
      241 aaagctaatt cttaatttta cacgactatt ttttctcaaa aaaacaggtc taatgaaatc
      301 ttctatttct atataatcta tagactcact taataatcgt gctgttttta aattcaaacc
      361 tgctgcgtaa aaattgtaat taaatataga aaagccattt gtaaattggg aaccagaagg
      421 tgtaaaatct tgattgtcaa tatgttgagt tgcaatataa gctgttctta gagcattaga
      481 aatagaaaaa tcaacactac ttctattaat ggacgtaaaa aaagttgtag cacaatcccc
      541 aatagcatat atatctttta tatttgtttg ctggtattca ttagttagat atgctccact
      601 aacatctctc aagagttcct ttttgccaat tggactatta gatagaaatc ctatagcttg
      661 tacgaccata tccatagagt attgggtacc acttgtagtg actatttttt caatcttttt
      721 tcttcctaaa tattttttag gactttctga tagacaaaga tgtattccat tttttgatag
      781 tatttcttca actttatctg aaaaactacg gtcgtaataa gtcgacaaca ctctatctgc
      841 aacatcgaaa agatatactt ctttccctct ttttcttatg gcttcagcaa tttcaactcc
      901 aatatatcct gctccaataa ttcctacttt tttaatagta ttatcatcta aagctttaaa
      961 aatctcttta gcatttggaa aattctttat cgaaaaaata ttagctaaat tattccccgg
     1021 tacatctaac ataatttgct ttgaacctgt agctagtata agtttgtcat attcttggga
     1081 aaatcttgtg ccatcttttt ttacacaaat aatcttttta ttttgaat
//
LOCUS       DAYYKN010000274         1088 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000274 DAYYKN010000000
VERSION     DAYYKN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.283"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1088
                     /locus_tag="ACHBB7_002956"
     rRNA            <1..>1088
                     /locus_tag="ACHBB7_002956"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          287 a          250 c          332 g          219 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagc
//
LOCUS       DAYYKN010000275         1058 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000275 DAYYKN010000000
VERSION     DAYYKN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.284"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..808
                     /locus_tag="ACHBB7_002957"
     CDS             <1..808
                     /locus_tag="ACHBB7_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9210831.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPI
                     EFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLT
                     TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT          385 a          179 c          257 g          237 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgca
//
LOCUS       DAYYKN010000276         1030 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000276 DAYYKN010000000
VERSION     DAYYKN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.285"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..252)
                     /locus_tag="ACHBB7_002958"
     CDS             complement(<1..252)
                     /locus_tag="ACHBB7_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210832.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSD"
     gene            complement(254..>1030)
                     /locus_tag="ACHBB7_002959"
     CDS             complement(254..>1030)
                     /locus_tag="ACHBB7_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM9210833.1"
                     /translation="IFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKI
                     KWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQH
                     SSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELK
                     RAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFIL
                     SIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          394 a          176 c          125 g          335 t
ORIGIN      
        1 atccgagaaa aattgattat ggttagttaa caagttaatt ttacgtttaa aggaagaacc
       61 gattttccgg tgattcacaa aaacaacatc attaaatagc cctaattctt ttatacgctc
      121 actatatctt ctagcgtttt caaattcaga ataaataaat aaatctcctt ttgattctcg
      181 gtaaaaattt tgataaacat ttaatatatt aatcaaatgt aaaggagtcc aagcaatgaa
      241 agctgtcttc atattaattc tcctttttta aatacaagta agaaatgctt cccaaataat
      301 accagaaaac aattgaaatg acatcgcgct tattgaaaag tacatgagcc tccaccattt
      361 catctataaa aatagatagt ataaaaatta aaacaatacc aagaaacgca taattcactt
      421 tacttctaaa aaatgttttg agtaaactaa agatatttaa cacatagaaa gcatagatac
      481 atataaaagc aattatacca cttgatacaa gtacttgaag aaatccatta tgcatgtttc
      541 catgtgctct ttttagttca ttcacatctg tagtatcaag atctactttt ttcaaaaatt
      601 ttgcatcgct attcccatag aaatcggtct ctccataacc aaataacaaa gttggtctta
      661 taactttaag cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg gagttatcac
      721 tagaatgctg taatattact tctttttctg gaagtggagt tttatctgat tcatttgact
      781 ctataacttc tacttttcta tcaatcgata caggaattgt tgctaaaaaa tccttagcat
      841 aattagtacc aaaagaaaaa tataccaata gaactaataa tatgcttacc atccatttga
      901 tttttacagg tagtacttct ctaatgctct ttctaaattt tgaattggca aacaacaaaa
      961 gaaataatac taaaaaagaa caaattacta cctgggcacc tctagacgaa gaaagtgtaa
     1021 tataaaatat
//
LOCUS       DAYYKN010000277         1001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000277 DAYYKN010000000
VERSION     DAYYKN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.286"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..936
                     /gene="bsh"
                     /locus_tag="ACHBB7_002960"
     CDS             <1..936
                     /gene="bsh"
                     /locus_tag="ACHBB7_002960"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM9210834.1"
                     /translation="FGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLDTHYAMIGIAAG
                     IADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGEA
                     KKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPS
                     FDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTK
                     LNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTY
                     EDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          361 a          126 c          176 g          338 t
ORIGIN      
        1 tttggaagga attttgatta tgaaatatct tacaatgaag tagttactgt tactccaaga
       61 aattataagt tgaattttcg aaaggtaaat gatttggata ctcattatgc aatgattggt
      121 attgccgctg gtatagctga ctaccctctt tattacgatg cgacaaatga aaaaggattg
      181 agtatggctg ggctaaattt ttctgggtat gctgattata aagaaataca agaagggaaa
      241 gacaatgtat ctccttttga atttattcct tggattttag gacaatgctc aacagtagga
      301 gaagctaaaa aattgttaaa aaatatcaat ttagcaaata taaattatag tgacgaactt
      361 cctttatccc ctttacattg gctattagct gataaagaaa aatcaattgt cattgaaagt
      421 atgaaagatg gacttcatat atatgataac cctgtgggcg ttcttaccaa taatccttca
      481 tttgactatc aattatttaa tttaaacaat tatcgtgtct tatcgagtga aactcctaaa
      541 aataattttt caaatcaaat aagtttgaat gcctatagcc gcggtatggg agggataggc
      601 ttgcctggag atttatcctc agtatctcgt tttgttaaag cgacttttac gaagctgaat
      661 tctgtatctg gagattcaga gtcagaaagt attagtcaat ttttccatat cttaggttca
      721 gtagaacaac aaaaaggttt gtgtgatgta ggtgatggaa aatatgaata tacaatttat
      781 tcttcttgtt gcaatgttga caaaggaatc tattattatc gaacatatga agacagtcaa
      841 attactgcaa ttgatatgaa taaagaagac ttagatagtc ataagttaat tagttatcca
      901 attatagaaa aacaacaaat taaatatata aattagttaa tgtgttgtga ttgattattt
      961 aatatagata taatacaaaa aggcaaagat attttcaaaa c
//
LOCUS       DAYYKN010000278          997 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000278 DAYYKN010000000
VERSION     DAYYKN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.287"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..407
                     /locus_tag="ACHBB7_002961"
     CDS             90..407
                     /locus_tag="ACHBB7_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM9210835.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(498..863)
                     /locus_tag="ACHBB7_002962"
     CDS             complement(498..863)
                     /locus_tag="ACHBB7_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210836.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          373 a          125 c          160 g          339 t
ORIGIN      
        1 tttttaaaac atcacaagga aaaacttaac tatggtatta tctaaacata attttttcgg
       61 tatttaatat ttaattagga ggtaatattt tgctttttta tagtaatttt atcttaatag
      121 tagcgatatt attacttctg aatatatgga tttttgatag gagtagaaat tcaagtatag
      181 gttttagaac taaacgaagt ttgtcttcta aaaaaaattg ggtatactca caaactattt
      241 tttatggagg aattgtttta attagtttac tttcttcaac actttattct ttgaatatta
      301 tagatgtttc cacctcaaat tcaatatcta ttattggaat cattattgct gcaattatta
      361 cacaactgtt tttagtgttt ggagagaaga aaagaagtaa aaaatagaaa aactagtgat
      421 agttttggtg atataagaaa aacatatatg tataaaaaga agaaagtagc ttctctttgt
      481 acatatatgg tttgttttta tggatgccga gcttttttat aagataaggc taccgcccat
      541 aacgacccaa ggatttgtgg aactaagtaa atacctgcaa gaagcaagat cagctgggtc
      601 ggttttaaag gatgatagaa gtaacgccat tcagtaaaat tggcgcaaaa taaataggaa
      661 gtgacagtag atataatatt ggcaatgaat aaagatataa tacgcctgtt cctttgaaaa
      721 taatatccag caaaaagaga taaaaatatc aatgtgatga acccgcttac ggaatgataa
      781 cgaaaatcaa gcagtaaggc gaaatgcaca taaggaacaa aagataaaag caacggaagg
      841 tttttccagc aagtttttat catctttcat caccacctaa caagtgttat tattttgtta
      901 gttttattgt aaattgaatc ctgcaataat caattacttt ttcattattt ctagattaaa
      961 tgaataaact ctcatgccat tatctctaaa aaaaaag
//
LOCUS       DAYYKN010000279          990 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000279 DAYYKN010000000
VERSION     DAYYKN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.288"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            359..990
                     /locus_tag="ACHBB7_002963"
                     /pseudo
     CDS             359..990
                     /locus_tag="ACHBB7_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          339 a          180 c          182 g          289 t
ORIGIN      
        1 agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa caccttcaat
       61 tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt aaacgaatta
      121 ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag tgcaaatatt
      181 aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga tctatatatt
      241 catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata taggctctat
      301 aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt ttaggtatat
      361 gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa ggaccctgcc
      421 aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata aaatgagctt
      481 tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac gaaaattgtc
      541 tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat atttagtgac
      601 agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg cgaaagtcgt
      661 cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt ttcgtgtaca
      721 agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag tgaaaaagaa
      781 ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca aaacatggcg
      841 cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg atcgattgct
      901 ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga ccgaagcctt
      961 tagagacaag gaatcagact tattttttac
//
LOCUS       DAYYKN010000280          984 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000280 DAYYKN010000000
VERSION     DAYYKN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.289"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..446
                     /locus_tag="ACHBB7_002964"
     CDS             36..446
                     /locus_tag="ACHBB7_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9210837.1"
                     /translation="MIKVYTSPSCTSCRKARAWLKANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            460..816
                     /locus_tag="ACHBB7_002965"
     CDS             460..816
                     /locus_tag="ACHBB7_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9210838.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          338 a          151 c          196 g          299 t
ORIGIN      
        1 gtagaattgg attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta
       61 gctgtacttc ctgtagaaaa gcaagagcat ggttgaaagc gaatcaactt gaatttgaag
      121 agaagaatat ttttgccgaa ccgttaacag aaagtgagat caaaaagatc ttggcagcga
      181 ctgaaggagg agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca
      241 tagattttaa cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat
      301 tattgagacg acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg
      361 agatccggtc atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac
      421 aaaacaataa catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga
      481 tcagcgcact gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct
      541 tatcaatttg ttgacttaga agaacgcgat ttaagtacag aagaagcaga agaattatgc
      601 agttttgaag aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac
      661 attgcgattg acctttcctg tgtaggaaaa aaacaactga tttttttatg cacacaacat
      721 accaatctcc tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat
      781 gagcagttga tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca
      841 ctgagcattt catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct
      901 ttttttgact ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa
      961 tctaggtggg gatatagagt taaa
//
LOCUS       DAYYKN010000281          969 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000281 DAYYKN010000000
VERSION     DAYYKN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.290"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            359..505
                     /locus_tag="ACHBB7_002966"
                     /pseudo
     CDS             359..505
                     /locus_tag="ACHBB7_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(593..886)
                     /locus_tag="ACHBB7_002967"
     CDS             complement(593..886)
                     /locus_tag="ACHBB7_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM9210839.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          291 a          166 c          145 g          367 t
ORIGIN      
        1 gaataaaaga gttaggacgg gattatctac tatttttctt tgttttaaat atttgttatc
       61 tttgaacata aaagagaaat gtgtcgactg cagtgttgtg tccctcatca cgaataaacg
      121 gtgttcaatt agctgatctt gtattcactt aaaaaatggt aaagtacgag ttgctattcc
      181 ccattttcaa ttttctaacg ctcaggacaa aatactgttt tcacaacctc attggaataa
      241 aagcatatat attgtttcaa gaacgaaatc ttgttatctt tttaaagggc atctgtccaa
      301 aatctaattt taggaggtac gttttggtat ttattcgtac aaataaaaga gaattatctt
      361 tattcatgta acgcatccaa ttgatttttt catgtacgct cctggaacga ttttactgac
      421 gattgcttat ttaactgcga atcgctcatt agcttttatc atagctatcc atattttgaa
      481 taacgttctt ggctttgtat tatagataca gatgacaaaa aaagttcaag tatgcggtgt
      541 agaaccaata tcgcatactt gaactttttt caatgagtat aaattaaaac ttctaatttt
      601 caattcccat catctgatat gcccgaccgt ctgaacttct tttgaaaaag ccaaatgtga
      661 ttagataacg tctttgttcg acataatcgc cactagaaaa tacgttcagc agtatcgaat
      721 taacctctgt ttctgtataa gtcactccat ggtcaaagcg tttcaagact tctttcaata
      781 ggatgtattt cttttttgtt gaacttggta cttgttttac ttttccatca ttcatgaatg
      841 tgtctaaaac tttcatcttt tcttgctccg tataagtata aaccatcttc tttctccttt
      901 tgattctaag aatactccat ttatgttaga atattctgga ttttttatta aagcggttac
      961 tcttaagaa
//
LOCUS       DAYYKN010000282          963 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000282 DAYYKN010000000
VERSION     DAYYKN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.291"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..573)
                     /gene="aac(6')"
                     /locus_tag="ACHBB7_002968"
     CDS             complement(25..573)
                     /gene="aac(6')"
                     /locus_tag="ACHBB7_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM9210840.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          292 a          190 c          140 g          341 t
ORIGIN      
        1 tacttctgtc acaacctctt tttattattg agaatttggt cgaggaataa tcgtttttgc
       61 catccaaata tccggtttgt cccagccatt tgcatttggt aagacaccta cgattttata
      121 acctaatttt tcatagaatt catacggatg ttcacgaagg ttctggatgg aagccacttt
      181 atcaaatgta tgctcataca ggtccgtttg acttaacgtt gttccatggt ctaaatcatc
      241 cgtacctaaa taaatcgtga ttcctcctct ggaagctact tctttttcta agtaattgac
      301 taatcgagta cctatttggt tctttcgtcg ggagctttct acaactaatg gatgcaattc
      361 ccaacctgtg ataccgtatt gagggattgc accaataaat cctactaact catcttggtc
      421 taccgctgct accgcgattc gttctggatt catcatttct tctacttctt ctgccgagct
      481 gtctccatat tcttccggcc aagtcagtct cagtaaatca gaaagctgat ctttcaatac
      541 tggattatta cggtcaaatt cactgattat catacgtctt cccccttgtt cttgcttatt
      601 ctaacagtcc ttttgaaaaa acgaaatgtt ctcgctcaaa aatctttagc taccaatcag
      661 tatctctatt ctttcattat ttatcttact tataaaataa aaaattatac agaaacactt
      721 tattcaaaat aggaaagatc aggaaagcca tcaattggag gaaaaaactc tgtttgttca
      781 tcatgatata gattatcttt tgctttaaaa aaaaccaatt atgaattgac aaataaaacc
      841 tgttttcata taataatctt aatttttaga atttttagat aattacaaaa aagcaaaggg
      901 aatcggtaga aatactaaga ggaacaaaaa ttcattattg tcgttttcaa tgtttttgta
      961 ctt
//
LOCUS       DAYYKN010000283          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000283 DAYYKN010000000
VERSION     DAYYKN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.292"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHBB7_002969"
     rRNA            <1..>943
                     /locus_tag="ACHBB7_002969"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYKN010000284          922 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000284 DAYYKN010000000
VERSION     DAYYKN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.293"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>922
                     /locus_tag="ACHBB7_002970"
     CDS             <1..>922
                     /locus_tag="ACHBB7_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9210841.1"
                     /translation="VSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIV
                     FLLIGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFI
                     STFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWR
                     YALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNN
                     AWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSL
                     VVPITLGYFFQFLYSLPVNAEFYEKKTSFIA"
BASE COUNT          278 a          115 c          157 g          372 t
ORIGIN      
        1 gtatcaattt tttcgattct tgttgggcta agcctaaatt cggcaattaa taatgcaagt
       61 tttgactata aagatgacat aaatgaattt atgtcatcgg tactttctct agctacactt
      121 tcgttaattg tgttcttatt aatagggaac atatattttt tgttttcaaa tgcttttttt
      181 gatatgaacc aacttgtatt taatttgatg ttattacaaa gttttgctag ttttgtaatt
      241 agttatatcc agtcttattt tacgattaac tttaaccatt ttaaatattt gattttatct
      301 tttatatcta cttttttgaa tattgcaatc tctgtagctt tgatgcagac attatatttt
      361 gatgataaat atgttggcag agtagtcggc ggaacactgt catttgtgtt gataggttta
      421 gtaatttatt taagaataat gataaagggt gctcattttt ttgataaaaa acagtggaga
      481 tacgctttaa aaatctcttt gccgctaatc ccacattcct tagcaaatat agttttagca
      541 caattcgata gaattatgat taattcttat ttaggtcctg ctgcgggggg aatatattcc
      601 tacattagta acataggtat tattctaagc gtaatatgga tgtctactaa taacgcctgg
      661 gttccatggt tctatggtga gatgagtaaa aataatgaaa aaaaagtgaa gaaagttagt
      721 aactattatt tagtagtctt tactttgatt acgatgattg taatggtagt ttcgattgat
      781 ttagcaaaat ttatggctcc gaaagactat tattctgggt tatctttagt ggttccgata
      841 accttaggct atttctttca gtttttatat agtttaccag ttaatgctga attttatgag
      901 aaaaaaacat cgtttattgc ga
//
LOCUS       DAYYKN010000285          920 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000285 DAYYKN010000000
VERSION     DAYYKN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.294"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..>920)
                     /locus_tag="ACHBB7_002971"
     CDS             complement(56..>920)
                     /locus_tag="ACHBB7_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9210842.1"
                     /translation="GKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPEL
                     EEWQARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTE
                     SKNRWMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRY
                     LPSKDYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFD
                     YGSAIRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFM
                     KTNARSDSYDV"
BASE COUNT          285 a          194 c          163 g          278 t
ORIGIN      
        1 aactagatca tcaatcgtaa gcatcgcaaa ttgatttcta ccattcgtat tgtttttaga
       61 catcatagct atcactcctt gcattcgttt tcataaatct attataaaag aaaagactgt
      121 agacatcatc cataaaatgg actttgtcta cagtctgcgc attctcatta gagaacactc
      181 ccttttttgt gacttttctg aagctagagt gaatactttc aactgcatta gtagtgtaca
      241 tgatttttct aatggcgctg ccataatcaa agagttgttc aacatgagaa aagtttcgtt
      301 tgcatacatc tactgcacca gaataatgag accaccgatt ttgaaaactg tcgaaggcag
      361 cgtgtgcagc gtttaaagaa gaagcactgt agaatttttt catgtctcga cagacttcct
      421 tataatcctt acttgggagg tatcgtaacg catttcgaac aagatgaaca atacagcgct
      481 gcacaatgac agaaggaaaa attgcttttg cgccttcttt gagaccggaa acaccgtcca
      541 tcgaaatgaa aaaaacatct tctacaccac gtactttcag ttcatcaaat acttgcatcc
      601 agcgattttt agattctgtt tgattgagcc ataggcctaa aatctccttg tttcctttaa
      661 gatcatagcc aagaatggtg tacacagcat attctttagc ttcatagttt tcgcgtaaag
      721 tgacgtacat acaatcaacg aatagaaatg cataacactt ggctaatggc cgagcttgcc
      781 attcttccaa ttcaggaagg acggcatccg tgatatcaga aatcatttcg tgggaaatat
      841 caaagccgta aatatcttca acggttgcgg cgatatcgca ttgactcatt cctcgtgcat
      901 acatggaaag aaccttacct
//
LOCUS       DAYYKN010000286          905 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000286 DAYYKN010000000
VERSION     DAYYKN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.295"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..905
                     /locus_tag="ACHBB7_002972"
                     /pseudo
     CDS             1..905
                     /locus_tag="ACHBB7_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          330 a          157 c          172 g          246 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacac
//
LOCUS       DAYYKN010000287          898 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000287 DAYYKN010000000
VERSION     DAYYKN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.296"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>898
                     /locus_tag="ACHBB7_002973"
     CDS             4..>898
                     /locus_tag="ACHBB7_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM9210843.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHY"
BASE COUNT          298 a          169 c          181 g          250 t
ORIGIN      
        1 tatatgaatg attctatcaa aaaaatgctg agaataacag agaaagattt gatgattaca
       61 gaggtctctt acgagaccct tcagaagaaa aagacgttgg tcgtcgatgc tgttctctcg
      121 cctactcctc gtgcttgtag aagttgtggt tctactgtgg tagatggaaa cgggaaagca
      181 attatagtga aaaatggaaa aaaggaaacg attgtccgtt ttgaacaata caatcatatg
      241 cctttggtta tgcgcctaaa aaagcaacgc tatacctgta aaaactgtcg cacccattgg
      301 acggctcaaa gttattttgt ccaacccaga cattcaatcg caaatcatgt tagatataaa
      361 attgcttctt tactgactga aaaagtatct ttatctttta ttgcgaaaag ctgtcaggta
      421 tctttgacca ccgttattcg tacattgaaa gagtttaaaa gctatttacc aaagcaatct
      481 aagaagatcc tccccagagt attgatggtt gacgaatttc gttcgcatgc ttccatagaa
      541 gataagatga gctttatttg cgcggatggc gaaacaggaa aattaataga tgttttgcct
      601 acgcgtaaat tacctcgatt aacaagctat ttcttaggtt gtaccaatcc agaagaagta
      661 gaattcttgg tgacagacat gaacgccgcc tacttccagc tcaccaaacg tgttctgcca
      721 aatgcgaaag tcgtgattga tcggtttcat attgtcaaac acatgaatca agcgttcaat
      781 gagttgcgta tccgtgaaat gaatgaactt cgtaaagccg gacagaaaag ccaggcagaa
      841 aaactgaaaa agaactggcg ctttttgcta aaaaatcgtg caaacatcaa ccattatg
//
LOCUS       DAYYKN010000288          875 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000288 DAYYKN010000000
VERSION     DAYYKN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.298"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..243
                     /locus_tag="ACHBB7_002974"
     CDS             <1..243
                     /locus_tag="ACHBB7_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210844.1"
                     /translation="LNLKMFIIEKWIVIIAKIFSSISIIIPMTLTFSKEQFNVATTFV
                     SFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            complement(302..472)
                     /locus_tag="ACHBB7_002975"
     CDS             complement(302..472)
                     /locus_tag="ACHBB7_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210845.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(472..771)
                     /locus_tag="ACHBB7_002976"
     CDS             complement(472..771)
                     /locus_tag="ACHBB7_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210846.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
BASE COUNT          257 a          175 c          130 g          313 t
ORIGIN      
        1 cttaatttaa aaatgtttat aattgaaaaa tggatagtta ttatcgcaaa gattttctca
       61 tcgatatcaa ttatcattcc aatgacattg acgttttcta aagagcaatt taacgtagct
      121 actacattcg tatcttttat agctctattt tcgtttgctg ttcctaaaac ttttcaaagc
      181 aaatctagca acgcaaaggt taatgtagaa aacactgata gaaaaaatga tgaaagttat
      241 taaaaaaaat aattctttca tttattgtca aacctttctt atagacatta gactttttta
      301 gtcagtccct cccgactggc ttttttgttt tgtactcagc ttcatccagc cctataaaaa
      361 tccaaaccat gtacacaatc gtacctatca acgcttgtct gcttccccag aggcctaaag
      421 cgtagatgat tagcggtgcg ctgaatacta gtgctctgtt gaatttgccc attaacttgc
      481 ctccttcaaa acatctgaag gcgataaact ttttacacga tatttgtttg cgtctttcca
      541 ctttaaaaac caaataaaag tatggtaatg aataaatgtg gttgagtgac ctggacgaag
      601 tattccttct gcaaattcag gaattgtttc catttccttg cagtattcaa ctaaagtagt
      661 ttttgacatg ccgtggaact tctccaagat taaactttgt cggtaccagt cgtctggttt
      721 tactgccttc tcagcagctt ctatcagctc tgaaagtgtt ggttttttca cttattatcc
      781 ctcctaaaaa ttatcgttcc atactcgatg tttaacattt cttgaagctc tgattttgta
      841 agaatgattt tatcttccaa cgactttacc tccct
//
LOCUS       DAYYKN010000289          801 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000289 DAYYKN010000000
VERSION     DAYYKN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.299"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(333..446)
                     /locus_tag="ACHBB7_002977"
                     /pseudo
     CDS             complement(333..446)
                     /locus_tag="ACHBB7_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          287 a          125 c          127 g          262 t
ORIGIN      
        1 tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata
       61 aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa
      121 aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc
      181 aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct
      241 tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
      301 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
      361 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      421 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      481 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      541 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      601 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      661 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      721 atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc
      781 ctttattcta tgctttatat t
//
LOCUS       DAYYKN010000290          792 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000290 DAYYKN010000000
VERSION     DAYYKN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.301"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..717
                     /locus_tag="ACHBB7_002978"
     CDS             1..717
                     /locus_tag="ACHBB7_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM9210847.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          200 t
ORIGIN      
        1 ttgaaaaaaa ggattttagt aacaattggt ctagcttttt tgttagctgg ttgtaccaac
       61 gggaatgaat cgaaaaaaga atcaacaaaa gaaacagcga aagcaacagt tgcaactact
      121 acaacaacta gcacagagag caagtcaact gcagcaagta cagaaacagc agatacctct
      181 acttcgtcag agtcttccaa aacgactgaa acaactcaaa catctacttc aagttcttct
      241 gtagaggctt catcttcctc ttcagaaagc agcgaacaag ttgaacaaac actgtggaat
      301 gcagacaaat ccgcacaatt agaggcattt atgaccaaat ggggccaaac aatgggacaa
      361 caatacaaaa gttatacgaa tcaaatgagt gtggacttat atggtcttaa agtccctcaa
      421 gctattttga acggggagtg gaagatggct attggtggag tgcctgtttc tgcagaatgg
      481 tcagaaagtg ggacaggtca agcagactat caaatcacag ccgtctattc cgatgcagag
      541 acagaaccat atctgaaaaa gcacgtctat ctattcgggt ttcagcaaaa tcagccaaaa
      601 gtattggtga cacaacaaaa tcaggggaat cccgataatt atctttactt caacgagaca
      661 gcgaataatg aattgaaaaa tggctttaat caaatcgttt atggacaagc aaattaatga
      721 gataaacaag gggttgtgac atgtctttta tcacaacccc ttgctcagtt tatttgttag
      781 tcttttattt tt
//
LOCUS       DAYYKN010000291          765 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000291 DAYYKN010000000
VERSION     DAYYKN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.304"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(238..>765)
                     /locus_tag="ACHBB7_002979"
     CDS             complement(238..>765)
                     /locus_tag="ACHBB7_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210848.1"
                     /translation="GLIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLV
                     ELDVNNYRELYVFNSLDVEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKNIKKCDK
                     CFESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIK
                     KAFKIKTIGTAPNFK"
BASE COUNT          261 a          103 c           85 g          316 t
ORIGIN      
        1 taacaataaa tgaagacttt tttattgtta attgtttatt tttctattat tagatatgaa
       61 aatcgcattt agataaaata gaaaaatatg aatatatttt aaatattaag atgagagtca
      121 ttaacttatg atagtagtca atttgctttt cactaattac aaaatatgtg atataggaag
      181 tttagttatt gttctcataa tgccatcctg tttttagatt atctagtaat ttttttgtta
      241 tttaaaatta ggagcagtgc caattgtttt aattttaaag gcttttttta tatcttctaa
      301 attagaagat atttgtatat caatatggaa tatattacct tttttgtatt tttttatgtt
      361 atctttagct aaaacatcaa atacatgtcc ttgaaattgt atcgaggcag ctggatttag
      421 tttaaattga ggaaacttta ccacagctga ttcaaaacac ttgtcacatt tttttatatt
      481 tttacctttg taaaaatatt cgattgaata ttctatttgg cacgactcac ttggcagtat
      541 atgtatgtca atatttttca tctgtttcat aggtatttca acatctaaac tattaaaaac
      601 atataattcc ctgtaattat ttacatctaa ctctactaaa tttatctttg gaacaataga
      661 tcctccaccc attccaccat aataaaaaaa atcttgttct ctatcaaaat agcacatatt
      721 aagctcagtt tgtttatcat cctcaataat tttataaatc aaacc
//
LOCUS       DAYYKN010000292          760 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000292 DAYYKN010000000
VERSION     DAYYKN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.305"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..482
                     /gene="ssrA"
                     /locus_tag="ACHBB7_002980"
     tmRNA           116..482
                     /gene="ssrA"
                     /locus_tag="ACHBB7_002980"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(528..>760)
                     /locus_tag="ACHBB7_002981"
     CDS             complement(528..>760)
                     /locus_tag="ACHBB7_002981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210849.1"
                     /translation="FEGTCLNHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEYVLG
                     ISKCLNVFLNAKSMQKILYFSTFLSLTKIKKR"
BASE COUNT          248 a          126 c          149 g          237 t
ORIGIN      
        1 aaaggggcag ttattatata aataatggga tatgattcgc ttttttattg aatttatccc
       61 aatatatgct atactaattt tgtatgaaaa atacacgtaa cccttgttag ctaacgggga
      121 cgttacggat tcgacaggca cagtcgagct tgaattgcgt ttcgtaggtt acgtctacgt
      181 aaaaacgtta cagttaaata taactgctaa aaacgaaaac aactcttacg ctttagctgc
      241 ctaaaaacag ttagcgtaga tcctctcggc atcgcccatg tgctcgagta agggtcctaa
      301 ctttagtggg atacgtttca actttccgtc tgtaagttga aaaagagaac atcagactag
      361 cgatacagaa tgcctgtcac tcggcaagct gtaaagtgaa tccttaaatg agttgactat
      421 gaacgtagat ttttaagtgg cgatgtgttt ggacgcgggt tcgactcccg ccgtctccat
      481 atatgtatgg ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt
      541 attttggtca aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc
      601 aaaaaaacat tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg
      661 tttacaaaaa gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa
      721 ggaaaaacac aattatggtt taagcatgtt ccttcaaata
//
LOCUS       DAYYKN010000293          732 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000293 DAYYKN010000000
VERSION     DAYYKN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.306"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            373..603
                     /locus_tag="ACHBB7_002982"
     CDS             373..603
                     /locus_tag="ACHBB7_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210850.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            609..>732
                     /locus_tag="ACHBB7_002983"
     CDS             609..>732
                     /locus_tag="ACHBB7_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9210851.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLI"
BASE COUNT          255 a          115 c          122 g          240 t
ORIGIN      
        1 cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt gaaaagcaat
       61 ttccgtgaat ttcttcttat ttcttggagt taaacgcttc tgttccagcc tctttttatc
      121 tgtctttcac taatgatcag ttgtagataa gcaagttgct ttttcttaca tcataccgcc
      181 catcaagaca aacgttttta gcttgtacgt actggacaaa aatcccatta tatcaagaaa
      241 aaacaacgtt gataatactt aaaattatta ggctagaacg gactagtaag agttttttgc
      301 cccttttttg cccctcaaat atcgctttac agccagaaca taagttcgta taatcgtttt
      361 gaggtgattt ttatgatgga agactttatt agaaaaaata tcagtgatga atatgcagac
      421 ttttatgagc aaagcagcaa aaaagataaa ttccagatgg atgtttcaat tttagctata
      481 ttagcttttt ccgaaaataa acaacctgta actgcaaaaa aagaaacagt attctctgaa
      541 ggcaaaataa aaactcgata tatattagag gtagaaacta agtttaaaaa tagatcggag
      601 taatggctat gctttttaat gagacgcaat tatggtttaa attcgatcct tcgaatagat
      661 ttgtcaaaga tttttataag gtgtgggatt cagaagtttt ctttttagca atcgaagata
      721 gcttattaat ca
//
LOCUS       DAYYKN010000294          687 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000294 DAYYKN010000000
VERSION     DAYYKN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.308"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>687)
                     /locus_tag="ACHBB7_002984"
     CDS             complement(<1..>687)
                     /locus_tag="ACHBB7_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210852.1"
                     /translation="EIIIPEFDSRNILLALVRYVNWEGYENKNPYVEEACNELIDYVE
                     THFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDKKSAETKPLFQLVCQGLEEQK
                     GILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVYKNFVLNDLSLNELILQIISI
                     AEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLSEKEQLLYGKIVEILEEWKKK
                     NQIQLRWNEN"
BASE COUNT          201 a          137 c           91 g          258 t
ORIGIN      
        1 aattttcatt ccatcttagt tggatttgat ttttcttttt ccactcttcc aatatttcta
       61 caatttttcc gtacagaagt tgttcttttt ctgatagaag atagatcctt tccggtaaaa
      121 atacttgtcc atctgcccac aacgtacgga caaattgtaa aaaggattgc ttgaaaataa
      181 aattttctgc atcgtcgatt tcagcgatac taataatctg taaaatcaat tcattcagtg
      241 ataggtcatt gagaacaaaa tttttataaa caacttcgaa atcttttctt aacagctcca
      301 tgttctcatt ggtataattt ctactgttga acaaagaaaa aatataatag acatcttctt
      361 cttgtaagtc tagaattcct ttttgctctt ccaatccttg acagactaat tgaaataacg
      421 gcttcgtttc tgcgcttttc ttatcttctt ctttcagtcc aaggggaaat gcctttcttc
      481 cgattgcgat ctctattcct cgtatgataa aggacttttc atcttccgaa tatcttcttt
      541 taaaaaaatg agtctctaca taatcaatca actcgttaca agcttcttct acatacggat
      601 ttttattttc atagccttcc caattgacat aacgtactaa agctagcaag atgttccgcg
      661 agtcaaattc tgggataatg atttctt
//
LOCUS       DAYYKN010000295          631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000295 DAYYKN010000000
VERSION     DAYYKN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.309"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>631
                     /locus_tag="ACHBB7_002985"
     CDS             <1..>631
                     /locus_tag="ACHBB7_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFM9210853.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGEREK
                     QFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEINCVVADAGYKTP
                     KFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLA"
BASE COUNT          211 a          116 c          126 g          178 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 agaaaaacaa tttgcttatt ctgcccatac ttcatgcgac gacaatggat ttattcttag
      361 cacaatcatc actcctggaa atattcatga cagtcaagtc gcctttcagc tcgtcaaaca
      421 atcgaagaga ctttttcctg aaatcaattg tgttgtcgct gatgctgggt ataagacacc
      481 taagtttgtt cactttttga ctcatttgaa ccttcgacct tgtttgcctt attcaaggcc
      541 caaagggaaa aaggggctat tatcaaaaaa tgagtttctc tatgacgagt attttgattg
      601 ttatatttgt ccgcaagatc aaatgctcgc t
//
LOCUS       DAYYKN010000296          591 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000296 DAYYKN010000000
VERSION     DAYYKN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.311"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..225
                     /locus_tag="ACHBB7_002986"
     CDS             <1..225
                     /locus_tag="ACHBB7_002986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210854.1"
                     /translation="VFRRKLIISKYSLKKLITIMPFLIYNTVIFKNKNAKSLYNNGES
                     TKNKKIITLLKKQMKDQVLLDLILSSLEGI"
BASE COUNT          204 a          106 c           64 g          217 t
ORIGIN      
        1 gttttcagac gaaaattaat aatttctaaa tattctttaa aaaagcttat aactattatg
       61 ccttttttaa tatataacac agttattttt aaaaataaga atgctaaatc actctacaat
      121 aacggcgaaa gcacaaaaaa taaaaaaatt attacgttat taaaaaaaca gatgaaagat
      181 caggttttat tagatctcat tctttcatca cttgaaggga tttaatgaac tactttttcc
      241 tctttgtcgt ttttatatat tcgtattcct tcaaacagta ggatagcctc tttcaatgct
      301 tgatatttga actgtccgtt acttaaaaat ttagatgtgc cgccctcttc ctagaacata
      361 ttctttttta ctttttcatt aattgacaga ccaatcgaac gaaactgtaa atttcgatag
      421 gataatgatt ggtcaaactc attcattttc aagacatctc gattcatatt caagatagga
      481 atctttttat caacatcact ttattcgtat tttcctctac cataaaagct tcacgctcca
      541 caaaattgtt ttatacacta ctattcagcc cattcaacga ccaatgaatt t
//
LOCUS       DAYYKN010000297          588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000297 DAYYKN010000000
VERSION     DAYYKN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.312"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACHBB7_002987"
     tRNA            complement(65..150)
                     /locus_tag="ACHBB7_002987"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACHBB7_002988"
     tRNA            complement(167..238)
                     /locus_tag="ACHBB7_002988"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACHBB7_002989"
     tRNA            complement(250..322)
                     /locus_tag="ACHBB7_002989"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACHBB7_002990"
     tRNA            complement(340..421)
                     /locus_tag="ACHBB7_002990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACHBB7_002991"
     tRNA            complement(429..501)
                     /locus_tag="ACHBB7_002991"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACHBB7_002992"
     tRNA            complement(505..577)
                     /locus_tag="ACHBB7_002992"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAYYKN010000298          585 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000298 DAYYKN010000000
VERSION     DAYYKN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.313"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>585
                     /locus_tag="ACHBB7_002993"
     CDS             <1..>585
                     /locus_tag="ACHBB7_002993"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9210855.1"
                     /translation="TFQTLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVHFLFIGR
                     IMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYHGYQSDI
                     RPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEAVIESKSGYLCERKN
                     SCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKF"
BASE COUNT          226 a           65 c          119 g          175 t
ORIGIN      
        1 aacatttcag actttacaaa ttgctaaaca aagtaaatac aagctattaa acggtgcagg
       61 agtagatgta gaacacttta gttatttaga atatcctaaa gatgagcaag ttcatttttt
      121 atttattgga agaattatga aagagaaagg tgtagatgaa ctttttgaag caatgaaaaa
      181 cttatatagt gaagagaaaa attgtgtact tgatgtcctt gggtattttg aagaagatta
      241 cggcataaaa attgaaaaat acgaaaaaga gggatggtta aaatatcatg gatatcaatc
      301 tgatattcgt ccctttgtag aaagagcaca ttgctttgtt ttgcctagtt ggcacgaagg
      361 aatggcaaat actaaccttg aatcagcagc atcagggaga ccaattatta caagtgatat
      421 cccagggtgt aaagaagcag ttattgaatc gaagtctggt tatttatgtg aaagaaaaaa
      481 tagttgtagt ctctataatt cgatgaataa atttctaaaa ttatcatatg agcagcgcat
      541 taacatggga aaaatgggga gaaattatat gattaaaaaa tttag
//
LOCUS       DAYYKN010000299          575 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000299 DAYYKN010000000
VERSION     DAYYKN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.314"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            258..575
                     /locus_tag="ACHBB7_002994"
                     /pseudo
     CDS             258..575
                     /locus_tag="ACHBB7_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          194 a          106 c           82 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctccccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttcc
//
LOCUS       DAYYKN010000300          571 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000300 DAYYKN010000000
VERSION     DAYYKN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.316"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          186 a           81 c           81 g          223 t
ORIGIN      
        1 tcttgatgtg atatccttgt cttttttgta atgtttttaa cttattgctt ttgcaaaatt
       61 tatttacctg ttttctggat caacgatagt gtacattatg ctatccttgt ttggatttta
      121 ggacgtttaa agaggcactt ttttacataa cttaatccat agacctttta agcatgctgt
      181 gagataataa taaagaagag tttaaagcgc accccaaacc acttccccat aagtgtatta
      241 cgctttaaac tcttttatat ttgaattcac taagaagcat accatatttt tgaaaaaaag
      301 tgagaaaaaa ggcttataat taggatttag agtaattaat tagatgttta ttttatagga
      361 ctgatactat agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg
      421 tgaataagat aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac
      481 ctttctggat caacgaaagt atactttata tagtcataac gccgatggta actatgcact
      541 gtaacgataa tgctgctaaa atatcttttt a
//
LOCUS       DAYYKN010000301          570 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000301 DAYYKN010000000
VERSION     DAYYKN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.317"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>570
                     /locus_tag="ACHBB7_002995"
     CDS             <1..>570
                     /locus_tag="ACHBB7_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210856.1"
                     /translation="YMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHIN
                     RALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVT
                     LITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILL
                     GFIKLYDSKKESWVMLAISMTLLIYTHVL"
BASE COUNT          172 a           81 c           79 g          238 t
ORIGIN      
        1 ggtatatgaa tttgaaaaaa attttttctg taaaaatatt tatagtttat gcatttgtta
       61 gtttattata tttatttcca gcatttaaaa acatggaaat atttggcttt tatgatacgg
      121 catttcacat taatcgtgcg ttaagtttag aatctatttt tagctcccct attaattttg
      181 aaacgtttag atcatatggc atgcaagtaa ataattttta cccatggtta actctatatc
      241 ctttattttt attgatcaag tttactaatt tagcaatagg ctataacctt tttctttata
      301 ttgttacgct cattactcta tttatatgtc attatgtaat gtatgaaatt accaaaaaac
      361 atgttactag ctcctttttc gctattatat atacaacatc ttcttttagg agtgtagaaa
      421 tatttcttcg aggggcaatg ggggaattac tcgcaatgtc aatacttcct cttattttac
      481 tcgggtttat aaagctttac gactcaaaaa aagaatcttg ggttatgttg gcgattagca
      541 tgactttatt aatttacact catgttcttt
//
LOCUS       DAYYKN010000302          569 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000302 DAYYKN010000000
VERSION     DAYYKN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.318"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>569
                     /locus_tag="ACHBB7_002996"
     rRNA            <1..>569
                     /locus_tag="ACHBB7_002996"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          139 a          131 c          173 g          126 t
ORIGIN      
        1 gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccagtggc
       61 gaaggcggct ctctggtctg taactgacgc tgaggctcga aagcgtgggg agcaaacagg
      121 attagatacc ctggtagtcc acgccgtaaa cgatgagtgc taagtgttgg agggtttccg
      181 cccttcagtg ctgcagctaa cgcattaagc actccgcctg gggagtacga ccgcaaggtt
      241 gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa
      301 gcaacgcgaa gaaccttacc aggtcttgac atcctttgac cactctagag atagagcttc
      361 cccttcgggg gcaaagtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt
      421 tgggttaagt cccgcaacga gcgcaaccct tattgttagt tgccatcatt cagttgggca
      481 ctctagcaag actgccggtg acaaaccgga ggaaggtggg gatgacgtca aatcatcatg
      541 ccccttatga cctgggctac acacgtgct
//
LOCUS       DAYYKN010000303          527 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000303 DAYYKN010000000
VERSION     DAYYKN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.320"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..507)
                     /locus_tag="ACHBB7_002997"
     CDS             complement(25..507)
                     /locus_tag="ACHBB7_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210857.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
BASE COUNT          200 a          104 c           83 g          140 t
ORIGIN      
        1 tgttttttta ctgatgatcc accatcaata gttatttttt atagatacag atcgttcact
       61 ttttatcctg atttctgtcc agaagtaaaa aaatgttgca agaataaatg aggcaattac
      121 taaatagaac catggtttga tcgtcaagaa atacatggag gaataagcta aatccttcaa
      181 tccactctcg ctcaaaccat tagggccgat aatcaagcga ggcagcaaag aaaacgtaga
      241 tgtaaatact aataagcaag aaaagtaaag cagctgccaa ttgtagtgat tgatcagaga
      301 ttttggtaat aagagaccta cacttatcac taaatagaaa acaatcaaag ctggaaaatt
      361 cctagaaacc acaaatccgc ttccttggat caggcctaat atctccgcac cttgtacaac
      421 agcatttacc catgtaaaag gtagcaaaac gataggaaaa aaccaaaaaa ataaataaag
      481 ccgatcgatt actgcttttc ttctcattaa cgttccccca aactact
//
LOCUS       DAYYKN010000304          526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000304 DAYYKN010000000
VERSION     DAYYKN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.321"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..435)
                     /locus_tag="ACHBB7_002998"
     CDS             complement(136..435)
                     /locus_tag="ACHBB7_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9210858.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          167 a          100 c           63 g          196 t
ORIGIN      
        1 ataagataca atataaggaa acatctgttt ctttatttag aataaaaaaa tacagactct
       61 gttcaaataa atgaacctag cctgcattat gagattgact atacttttta attcattgag
      121 atgaataact cgtttttata aatgcccaaa agtaatcggt gccgaagcca ttcctcgacc
      181 taattctttt tcaaattttc ctattgttga tatctccata taaaacggtg atactggttt
      241 agacaattct cccctgattg ctaacggacg taaactacgt tccaaattat ttatgacttt
      301 aggataatac tgaccttttt ctaagcctgt ctttgcgtct atcaaaagta tacgttcctt
      361 ttctttgatc tctggattta aaacgagatc atataatttt agtaccatgt tttctatttc
      421 ttcactttta gacatcacga tttcctccca aaaatatatt tcttcccact acattctata
      481 aacaaagtat acgctacatc tagatttttt taaatcatta ctggat
//
LOCUS       DAYYKN010000305          526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000305 DAYYKN010000000
VERSION     DAYYKN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.322"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..475
                     /locus_tag="ACHBB7_002999"
     CDS             5..475
                     /locus_tag="ACHBB7_002999"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM9210859.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          186 a           99 c           77 g          164 t
ORIGIN      
        1 ctttatgtct atttataatt ttgaaactac tttagaagac ggcactgtct attcattgaa
       61 aaaatatgca ggcaaaccga taatcattgt taacactgca acaaaatgcg gtctagcacc
      121 tcaatttaaa caattagaag aactttacca agcttatcag acacaaggtc tggttatctt
      181 aggctttcct tccaatcaat ttaaacaaga gctagattca gcgactgaag cagcagaagc
      241 ttgtaaaacc acttatggcg ttaccttccc tatgcatcaa ttgattgacg taaatggaga
      301 aaatacagat cctcttttta cttatcttaa aaatgaagct cctggtactg tcactaaagc
      361 aataaaatgg aattttacta aatttttgat tgatagaaat ggcaaagtaa tcgaacgatt
      421 tgcaccacaa acttctccat tgaaaatgac cgctgcaatt gaatccgttc tttaacttaa
      481 taaaaataaa aaaagatgaa ctatttctag ttcatctttt tgcctt
//
LOCUS       DAYYKN010000306          493 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000306 DAYYKN010000000
VERSION     DAYYKN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.324"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..424)
                     /gene="spxA"
                     /locus_tag="ACHBB7_003000"
     CDS             complement(26..424)
                     /gene="spxA"
                     /locus_tag="ACHBB7_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM9210860.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          141 a          102 c           85 g          165 t
ORIGIN      
        1 cttttccaag tttcgtatta tccttctata atccagccat caattgtgct tgacgtaatt
       61 ctaattgtcg tacatcgcgc ggtaggaacc gacgaatttc atcttcatta aaaccaactt
      121 gcagacgttt atcatcaatc atgattggac ggcgaagcaa tccaggattg ttttgcacaa
      181 gttccaataa ttcttgtaac ggcagatcat ctaaatccat gttcagtttt tggaatactt
      241 tggaacgtgt cgagatgatt tcttctgttc cgtcttctgt catttgtaaa atcgctttta
      301 attcagaact gttcaatggt tctgaaaaaa tattacgttc aacaaatgga atctgatgtt
      361 cttgcaacca cgcacgagcc tttctgcagg aagtacaact tggggaagta tataatgtta
      421 acaacgtgca ccactccttt tcgtgataag aatcactttt ttgtaacaga agatcataat
      481 aatctcccat ctg
//
LOCUS       DAYYKN010000307          493 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000307 DAYYKN010000000
VERSION     DAYYKN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.325"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..>493)
                     /locus_tag="ACHBB7_003001"
     CDS             complement(54..>493)
                     /locus_tag="ACHBB7_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9210861.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT          133 a           92 c          115 g          153 t
ORIGIN      
        1 tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt tagttaatgg
       61 tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat agcgtgcata
      121 catttcagaa gcttgttcat acaagcaact gtggcaacct tatctttctt agggataggt
      181 tgctttttta atttgtagta ataatcgaca atatgattag gagctgcgcg ttgctggcga
      241 atcatatttc gaatggtgaa gaaaataatt ttccgtgctt tgggattacc acgcttattg
      301 atgcggtctt ggccagtata cttacctgaa tgataccgtc ggatgtcgat tccgacaaag
      361 gcattcaatt gtttatgggt tttaaatcgt gtgatatctc ctaattcacc gagcaataaa
      421 gcggctgtga gtgcgccaat cccagggaac gtttggtaca acaaaaagca aggagagcct
      481 tcggcttttt tga
//
LOCUS       DAYYKN010000308          476 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000308 DAYYKN010000000
VERSION     DAYYKN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.327"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>476
                     /locus_tag="ACHBB7_003002"
     CDS             22..>476
                     /locus_tag="ACHBB7_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9210862.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKK"
BASE COUNT          178 a           87 c           95 g          116 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaatattt ctgaacgtaa aaaaga
//
LOCUS       DAYYKN010000309          468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000309 DAYYKN010000000
VERSION     DAYYKN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.328"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACHBB7_003003"
     CDS             <1..>468
                     /locus_tag="ACHBB7_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM9210863.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAYYKN010000310          460 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000310 DAYYKN010000000
VERSION     DAYYKN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.329"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACHBB7_003004"
     tRNA            complement(20..91)
                     /locus_tag="ACHBB7_003004"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACHBB7_003005"
     tRNA            complement(113..200)
                     /locus_tag="ACHBB7_003005"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACHBB7_003006"
     tRNA            complement(225..298)
                     /locus_tag="ACHBB7_003006"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACHBB7_003007"
     tRNA            complement(312..382)
                     /locus_tag="ACHBB7_003007"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACHBB7_003008"
     tRNA            complement(384..459)
                     /locus_tag="ACHBB7_003008"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAYYKN010000311          443 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000311 DAYYKN010000000
VERSION     DAYYKN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.331"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..>443
                     /locus_tag="ACHBB7_003009"
     CDS             147..>443
                     /locus_tag="ACHBB7_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9210864.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDK"
BASE COUNT          161 a           75 c           61 g          146 t
ORIGIN      
        1 aaaacatttt tgaaaataaa catcagcagt cctatcaatg catgtgagct tatagaagtt
       61 attcgcagcg cagcataatt gcattagctt cttttcgtgt tttttgcctc ttttcccttt
      121 aaatcttaat ggaggaggtg tgcaatatgt cagataacga cgaacgcgta aatgcccagt
      181 tgattcgatt ctttgaaaag gtcattttga acaccgctaa tacttattat aaaaaacaag
      241 aagaaattca aaaacatgaa caacttgatc aactcccacc atatttgttt actaaagata
      301 ctataaaatt taaacaaccg attaatgttt taaatgtgac tctccttgtt gaagatataa
      361 aattagcaaa aatccttcct ttgttgaaag aacctgaaca aatttttctt atcgaaaaat
      421 ttatctttaa taaaactgat aaa
//
LOCUS       DAYYKN010000312          397 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000312 DAYYKN010000000
VERSION     DAYYKN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.332"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            184..333
                     /locus_tag="ACHBB7_003010"
     CDS             184..333
                     /locus_tag="ACHBB7_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210865.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          129 a           64 c           68 g          136 t
ORIGIN      
        1 aagttgaata accgcgattg attcactttt tgttttcttg tttatttgct tttatctgca
       61 acttttgact ttaaaatttc ttaactatga aaacctttca attggtggta tacttataaa
      121 tggaatttcg ttatcaacgc gttcgttgcc tttcagcaag tatttaaaga aaggagaatg
      181 gctatgacat tctataacaa agatatacaa tatcgagtaa ccttggatac agatttgaat
      241 ctatttgtcg tttttgacaa agaaaatacc aatcatgtag cgacaggtgt tacgatcgaa
      301 caagcagtgc aagaattaaa aaaatctgca taaggagtgg aaaccctcat atggacgatt
      361 ctcgttcttc tctttaaacg catctactgg tagataa
//
LOCUS       DAYYKN010000313          381 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000313 DAYYKN010000000
VERSION     DAYYKN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.333"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..94)
                     /locus_tag="ACHBB7_003011"
     tRNA            complement(21..94)
                     /locus_tag="ACHBB7_003011"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="ACHBB7_003012"
     tRNA            complement(108..181)
                     /locus_tag="ACHBB7_003012"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="ACHBB7_003013"
     tRNA            complement(209..281)
                     /locus_tag="ACHBB7_003013"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="ACHBB7_003014"
     tRNA            complement(301..374)
                     /locus_tag="ACHBB7_003014"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DAYYKN010000314          377 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000314 DAYYKN010000000
VERSION     DAYYKN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.334"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..341
                     /locus_tag="ACHBB7_003015"
     CDS             141..341
                     /locus_tag="ACHBB7_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9210866.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          136 a           57 c           70 g          114 t
ORIGIN      
        1 aagcatgata tattaaatga gtaatctatt ttgaaacgta atttgagcga tatatctaca
       61 tagataaaac tccttttgcc tatcaaatat ttattgcaac gaaaaaacgt acattgatac
      121 gtatcaggag gaatttattt atgaataacg gtacagtaaa atggttcaac gcagacaaag
      181 gttttggctt tatcactggt gaagatggaa atgatgtatt tgcacatttt tcagctatcc
      241 aaggtgacgg cttcaaaact ttagaagaag gccaagcagt gtcttacgat gttgaagatg
      301 gtcaacgtgg ccctcaagca acaaacattg ttaaagctta ataaaaattg acttttaacc
      361 gtcttttcga aagaaaa
//
LOCUS       DAYYKN010000315          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000315 DAYYKN010000000
VERSION     DAYYKN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.336"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHBB7_003016"
     CDS             complement(<1..>321)
                     /locus_tag="ACHBB7_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9210867.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAYYKN010000316          318 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000316 DAYYKN010000000
VERSION     DAYYKN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.338"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>318)
                     /locus_tag="ACHBB7_003017"
     CDS             complement(<1..>318)
                     /locus_tag="ACHBB7_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM9210868.1"
                     /translation="IDAPNGTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKAT
                     FTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYY
                     RTYE"
BASE COUNT           99 a           66 c           52 g          101 t
ORIGIN      
        1 ttcatacgta cgataataat aaatcccttt atcaacgtta cagcaagaag aataaatcgt
       61 atattcgtat tttccttctc cagtatcgca tactcctttt tgttgctcga ctgagcctag
      121 tatatgaaaa aattgactga tactttcaga ttctgaatca cctgatgcag cattcatttt
      181 agtaaatgtg gcttttacaa atcgagaaac agaagagaga tctccaggta agcccaatcc
      241 tcccatccca cgactataaa cattcaagct gatctggttt gaaaaatgat tttctggtgt
      301 tccattggga gcgtcaat
//
LOCUS       DAYYKN010000317          313 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000317 DAYYKN010000000
VERSION     DAYYKN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.339"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..293
                     /locus_tag="ACHBB7_003018"
     CDS             63..293
                     /locus_tag="ACHBB7_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210869.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          125 a           40 c           52 g           96 t
ORIGIN      
        1 cccctcaaat atcgctttac aacaagaacg taagttcgta taatcgtttt gaggtgattt
       61 ttatgatgga agaatttatt agaaaaaata tcagtgatga atatgcagac ttttatgatc
      121 aaagcagtaa aaaagataaa ttccagatgg atgtttcaat cttagctata ttagcttttt
      181 ccgaaaataa acaacctgta actgcaaaaa aagaaacagt attctctgaa ggcaaaataa
      241 aaactcgata tatattagag gtagaaacta attttaaaaa cagatcggag taatggttat
      301 gctttttaat gag
//
LOCUS       DAYYKN010000318          310 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000318 DAYYKN010000000
VERSION     DAYYKN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.341"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>310
                     /locus_tag="ACHBB7_003019"
     CDS             82..>310
                     /locus_tag="ACHBB7_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9210870.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQY"
BASE COUNT          112 a           46 c           65 g           87 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca
//
LOCUS       DAYYKN010000319          299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000319 DAYYKN010000000
VERSION     DAYYKN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.345"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>299
                     /locus_tag="ACHBB7_003020"
     CDS             <1..>299
                     /locus_tag="ACHBB7_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9210871.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAYYKN010000320          266 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000320 DAYYKN010000000
VERSION     DAYYKN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.348"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..>266)
                     /locus_tag="ACHBB7_003021"
     CDS             complement(34..>266)
                     /locus_tag="ACHBB7_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9210872.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaata
//
LOCUS       DAYYKN010000321          248 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000321 DAYYKN010000000
VERSION     DAYYKN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.349"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..>248)
                     /locus_tag="ACHBB7_003022"
     CDS             complement(44..>248)
                     /locus_tag="ACHBB7_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFM9210873.1"
                     /translation="KVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQI
                     RQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           63 a           62 c           49 g           74 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttg
//
LOCUS       DAYYKN010000322          235 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000322 DAYYKN010000000
VERSION     DAYYKN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.351"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>235)
                     /locus_tag="ACHBB7_003023"
     CDS             complement(<1..>235)
                     /locus_tag="ACHBB7_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9210874.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT           69 a           45 c           36 g           85 t
ORIGIN      
        1 atatcctaaa gaatatctca aagttccttc aaaacgtcgt tgtgctcgac ttaatagaac
       61 actgtaagcc agtaatatgc tttcaaatct actctctagc cctttaacag aacgtgagtt
      121 gaaattttga caacctaatt tttctaagga agaaaaaaca gtctcaactg tttttctttg
      181 ttttccaagt aagctgtggt ctagtttttc tgatttatcc atgtttttac gtggc
//
LOCUS       DAYYKN010000323          233 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000323 DAYYKN010000000
VERSION     DAYYKN010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.352"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAYYKN010000324          231 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000324 DAYYKN010000000
VERSION     DAYYKN010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.353"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>231)
                     /locus_tag="ACHBB7_003024"
     CDS             complement(<1..>231)
                     /locus_tag="ACHBB7_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9210875.1"
                     /translation="IDAALDFEFIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIR
                     SMRQTIKEVDTNVAYRWFLGYSFEEKIPHFST"
BASE COUNT           77 a           43 c           54 g           57 t
ORIGIN      
        1 aagttgagaa atgggggatt ttctcctcga aggagtagcc taaaaaccag cggtaagcga
       61 cattggtatc gacttcctta atggtttgtc gcatggaacg gataccgaat aaatactgaa
      121 taaacaccaa tttgatcaga atcactggat cgatacttgg gcgacctaaa tcggagtaag
      181 tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc t
//
LOCUS       DAYYKN010000325          231 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000325 DAYYKN010000000
VERSION     DAYYKN010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.354"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..201
                     /locus_tag="ACHBB7_003025"
     CDS             <1..201
                     /locus_tag="ACHBB7_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM9210876.1"
                     /translation="YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTR
                     KEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT           77 a           45 c           45 g           64 t
ORIGIN      
        1 tacctgaata atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat
       61 cgaagtttac gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat
      121 aagaaaaccc gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta
      181 ttattgggaa tcccagctta aatcatagat accgtaaggg attttattct t
//
LOCUS       DAYYKN010000326          224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000326 DAYYKN010000000
VERSION     DAYYKN010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.355"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACHBB7_003026"
     CDS             <1..>224
                     /locus_tag="ACHBB7_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM9210877.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAYYKN010000327          224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000327 DAYYKN010000000
VERSION     DAYYKN010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.denovo.356"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..93
                     /locus_tag="ACHBB7_003027"
     CDS             <1..93
                     /locus_tag="ACHBB7_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HFM9210878.1"
                     /translation="FRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT           82 a           42 c           32 g           68 t
ORIGIN      
        1 tttcgttata ttgtctatta ttgccataca aagataaaaa agaataaaca aaaagaaact
       61 gtgacaaaag tcttgtcaca gtcccttatc tgaataaacg gtgttcaaaa gccagtttct
      121 cggtattaag ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttac
      181 tactcgaaac taagcgactt tttcacaacc tctggtacac ggtg
//
LOCUS       DAYYKN010000328        10087 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.guided.096, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000328 DAYYKN010000000
VERSION     DAYYKN010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.guided.096"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            353..523
                     /locus_tag="ACHBB7_003028"
     CDS             353..523
                     /locus_tag="ACHBB7_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210879.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            537..1139
                     /locus_tag="ACHBB7_003029"
     CDS             537..1139
                     /locus_tag="ACHBB7_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9210880.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1155..1832
                     /locus_tag="ACHBB7_003030"
                     /pseudo
     CDS             1155..1832
                     /locus_tag="ACHBB7_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2143..2640
                     /locus_tag="ACHBB7_003031"
     CDS             2143..2640
                     /locus_tag="ACHBB7_003031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFM9210881.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            2641..3057
                     /locus_tag="ACHBB7_003032"
     CDS             2641..3057
                     /locus_tag="ACHBB7_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFM9210882.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3077..4621
                     /locus_tag="ACHBB7_003033"
     CDS             3077..4621
                     /locus_tag="ACHBB7_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9210883.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4764..4988
                     /locus_tag="ACHBB7_003034"
     CDS             4764..4988
                     /locus_tag="ACHBB7_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210884.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5003..5872
                     /locus_tag="ACHBB7_003035"
     CDS             5003..5872
                     /locus_tag="ACHBB7_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM9210885.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5853..6587
                     /locus_tag="ACHBB7_003036"
     CDS             5853..6587
                     /locus_tag="ACHBB7_003036"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9210886.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6620..7528
                     /locus_tag="ACHBB7_003037"
     CDS             6620..7528
                     /locus_tag="ACHBB7_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM9210887.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7525..8067
                     /gene="sat4"
                     /locus_tag="ACHBB7_003038"
     CDS             7525..8067
                     /gene="sat4"
                     /locus_tag="ACHBB7_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFM9210888.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8160..8954
                     /locus_tag="ACHBB7_003039"
                     /note="aph(3')-IIIa"
     CDS             8160..8954
                     /locus_tag="ACHBB7_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM9210889.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9230..9361
                     /locus_tag="ACHBB7_003040"
                     /pseudo
     CDS             9230..9361
                     /locus_tag="ACHBB7_003040"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9437..9930
                     /locus_tag="ACHBB7_003041"
                     /pseudo
     CDS             9437..9930
                     /locus_tag="ACHBB7_003041"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         3548 a         1572 c         2207 g         2760 t
ORIGIN      
        1 acaagaagca ttttattcta gcttaatttg acaaatcata ctatgatacg tgtttgtttt
       61 tctgtgctgt ttagtgaatg attagcagaa atatagagag taagatttta attaattatt
      121 agggggagaa ggagagagta gcccgaaaac ttttagttgg cttggactga acgaagtgag
      181 ggaaaggcta ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg atcttttaag
      241 ttgctatctt tttaaggtca atagagtata cttatttgtc ctaatatggt tttagcagta
      301 taattgactt ggtgaatagg tcatttaagt tgggcataat aggaggagta aaatgaaaaa
      361 atttatttat cgagttttag aaaatgacga agtggtggct atttttaatg agcaacaata
      421 tgcgcaagat tttatcgctt acgaaaagac aatttctgat aagcaatttg aaattgaaaa
      481 agtagatata gctgattggt tattgcaacc gagagaattt tagaggttgg ttgaaaatgg
      541 ctaaaattgg ttatgcacgt gtcagtagca aagaacagaa cttagataga caactgaaag
      601 cgttagaggg cgtttctaag gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc
      661 cacaattaca agctatgctt aactatattc gtgaagggga tatagttgtt gttactgaat
      721 tagatcgatt aggacgaaat aataaagaat taacagaatt gatgaatcaa attcaaatta
      781 agggggcaac cctggaagtc ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt
      841 taagacggct gattaataat ttagtgattg aattgtataa gtaccaagcg gaatctgaac
      901 gcaaacgaat taaagaacgc caagcccaag gaattgaaat tgctaagaaa aaaggaaaat
      961 tcaaagggcg acaacctaaa ttcaaagaaa atgatccacg tttacaacac gctttcgatt
     1021 tatttttgaa tggttgttcc gataaagaag ttgaagaaca aactggaatt aatcgccgaa
     1081 cgtttagaag gtatcgatca agatacaacg tgacagtaga tcaaagaaaa aaacaatgaa
     1141 aagagggata gttaatgagt acggttattt tagctgaaaa accaagccag gcattagcct
     1201 acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac
     1261 tatttacaga tgaaacgttt atcacctttg gttttgggca tttagtcgaa ttagcagaac
     1321 caggtcatta taacgaaaag tggcagaact ggaaacttga atctttgccg atttttcctg
     1381 atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgctgaac
     1441 ttctcaaaaa ggcaaataca attattgtcg caacagatag cgacagagaa ggtgaaaata
     1501 tcgcctggtc gattatccat aaagcaaatg ccttttctaa agataaaacg tataaaagat
     1561 tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag
     1621 gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga
     1681 tcggcatgaa cgcaagccct ttatatacct tgaatttaca acaaaaaggc gtacaaggta
     1741 cattttcatt aggacgtgta caaacaccga ctttatatct catttatcaa cgccaggaag
     1801 caatagaaaa ctttaaaaaa gaaccttttt tcctgaataa ttcatagttt tactaatagt
     1861 ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat
     1921 gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat
     1981 aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc
     2041 aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac
     2101 gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat
     2161 ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta
     2221 atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga ccgtgaaaga
     2281 atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca
     2341 ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat
     2401 agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg
     2461 gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat
     2521 ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg
     2581 gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa
     2641 atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag
     2701 gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg
     2761 gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac
     2821 gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa
     2881 gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct
     2941 ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca
     3001 gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt
     3061 gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa
     3121 agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga
     3181 acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa
     3241 tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa
     3301 gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat
     3361 tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca
     3421 gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc
     3481 caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct
     3541 ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa
     3601 ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga
     3661 agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg
     3721 cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg
     3781 accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca
     3841 aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc
     3901 ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc
     3961 gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat
     4021 ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta
     4081 tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag
     4141 aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg
     4201 tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact
     4261 ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa
     4321 caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta
     4381 tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga
     4441 agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa
     4501 gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt
     4561 tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg
     4621 aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc
     4681 tgattataat aatattttaa taactattgt gtttatcaac caaatggttt ataatattct
     4741 tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc
     4801 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
     4861 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
     4921 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
     4981 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
     5041 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
     5101 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
     5161 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
     5221 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
     5281 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
     5341 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
     5401 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
     5461 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
     5521 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
     5581 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
     5641 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
     5701 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
     5761 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     5821 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     5881 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     5941 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     6001 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     6061 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     6121 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     6181 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     6241 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     6301 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     6361 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     6421 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     6481 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     6541 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     6601 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     6661 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     6721 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     6781 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     6841 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     6901 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     6961 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     7021 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     7081 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     7141 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     7201 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     7261 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     7321 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     7381 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     7441 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     7501 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     7561 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     7621 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     7681 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     7741 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     7801 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     7861 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     7921 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     7981 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     8041 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     8101 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     8161 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     8221 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     8281 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     8341 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     8401 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     8461 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     8521 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     8581 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     8641 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     8701 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     8761 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     8821 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     8881 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     8941 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     9001 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     9061 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     9121 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     9181 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     9241 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     9301 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     9361 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     9421 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     9481 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     9541 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     9601 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     9661 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     9721 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     9781 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     9841 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     9901 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     9961 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
    10021 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
    10081 ttgtgat
//
LOCUS       DAYYKN010000329         8513 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.guided.116, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000329 DAYYKN010000000
VERSION     DAYYKN010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.guided.116"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1524
                     /gene="eat(A)"
                     /locus_tag="ACHBB7_003042"
     CDS             22..1524
                     /gene="eat(A)"
                     /locus_tag="ACHBB7_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM9210890.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1591..2118
                     /locus_tag="ACHBB7_003043"
     CDS             1591..2118
                     /locus_tag="ACHBB7_003043"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9210891.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            2329..3390
                     /locus_tag="ACHBB7_003044"
     CDS             2329..3390
                     /locus_tag="ACHBB7_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM9210892.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3393..4289
                     /gene="murQ"
                     /locus_tag="ACHBB7_003045"
     CDS             3393..4289
                     /gene="murQ"
                     /locus_tag="ACHBB7_003045"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM9210893.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4303..6285
                     /locus_tag="ACHBB7_003046"
     CDS             4303..6285
                     /locus_tag="ACHBB7_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM9210894.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6350..6796)
                     /gene="nrdI"
                     /locus_tag="ACHBB7_003047"
     CDS             complement(6350..6796)
                     /gene="nrdI"
                     /locus_tag="ACHBB7_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM9210895.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            7007..8431
                     /locus_tag="ACHBB7_003048"
     CDS             7007..8431
                     /locus_tag="ACHBB7_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM9210896.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         2737 a         1478 c         1913 g         2385 t
ORIGIN      
        1 ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac attcggctac
       61 gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac acaatggaaa
      121 ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat tctacaaaac
      181 aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt cccgcaacag
      241 acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga ttttgagata
      301 tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt atggagagaa
      361 ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt atttgtggat
      421 gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc tggtagaaaa
      481 caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg
      541 ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca actggaactt
      601 tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg
      661 gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa
      721 gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg attcatcgat
      781 actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg gacgatgaaa
      841 cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga aaaactatta
      901 aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca ccataagcga
      961 cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga gccaattcat
     1021 tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca
     1081 tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga aaaataccag
     1141 ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg aggaacgttg
     1201 gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa cctccgaaag
     1261 cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat gggccaacgg
     1321 aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac atgggatgaa
     1381 ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat cttgaacgtg
     1441 aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt atctactgag
     1501 attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg tatggttaag
     1561 gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca
     1621 acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac tgaaaaagga
     1681 cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa
     1741 atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc tgatcgtctc
     1801 agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga ttatgatgga
     1861 ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt acgattccca
     1921 aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa agaatgcatc
     1981 gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag agtgatcaat
     2041 gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga taatacagaa
     2101 ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt caaggcactg
     2161 cttttgctta gcagtgcctt ttttaggtta tgacaattct tttgataata taaactcaac
     2221 ctttagaaat gaaatttaat tattttaata aaagtattga aattttgttt ctttagatat
     2281 atactctagt catagaaatg aagacggata cagaaaaaga gggataatat gtttggcttt
     2341 tcaatattta tgaacgattc tttgtcagaa gagaagaaac agtatatccg ccaaatggca
     2401 tccaatggct ttgtcggtat tttcacttcc atgcatatac cagaagatga tgtttcggca
     2461 tatcaaaagc gattgatcga actaggaagc gaagcgaaaa agaataaact agaattgatg
     2521 gtcgatattt caggagaggc attggaacga gcgggttttt cattagaaga tctaacgcct
     2581 ttgaaagcaa ttggtgtaac aggtttaaga atggattatc atatttccaa tcagcagatt
     2641 gccgactggt cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac gcctaaagat
     2701 atcgatgaat tgaaagaagc agaagctgat ttctcacaga ttgaagcttg gcataattat
     2761 tatccacgtc ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa tcaatggctc
     2821 aaaattcaag gtctccttgt ccaaggtttt gtaccaggag atacagagtt gagaggccca
     2881 ctatatcaag gattgccaac tttggaagaa catcgaggga tccatcctct tgcaggcgct
     2941 ctagatctat tagcaagtaa cacggatatt gtttacatcg gagatgcagg tctgtcagaa
     3001 aacgttcagg agcaatttgc ttctttccaa aaagaacaga cagtcttgct ccatacggaa
     3061 cctgtagatg aagaatttta cgaatatata ttagggaagc atacgaatcg gcaagacgat
     3121 gcaagagatg tcatacgaag cgtagatgca cgttttcgag agattccgcc tatcccagca
     3181 agaaatacag ctacgcgtat gaaagggtct attactttag ataacgaaaa gtatttgcgc
     3241 tatatgggtg agatccaatt aaccaaatat gatttgccag cggatgaaaa agtgaatgta
     3301 gtagctaaag ttatcaaaga agagctacca ttgatcaacc aaatcaaagc tggtatgaac
     3361 tatcaattta taagaaaaga aggaagataa aaatgagcaa aatcaattta gagaacctaa
     3421 caacggaacg cagaaaccaa gaaacttttg gattagatga gatgagtatt tctcaagccg
     3481 ttcaactaat gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa gaactgacaa
     3541 gtatcgaacc agtgattgct aacacgattg agtcctttaa aaaaggtgga cgtctgattt
     3601 atatgggagc cggaacaagt ggaagactag gtgtgttaga tgctgctgaa tgcgtaccga
     3661 cattcggtgt agaaccggaa atggtcatag gtctaattgc tggtggagaa gaagcaatga
     3721 ctgtagctgt cgaaggggcc gaagattctt atgagctagg gcaagaagat ctaatcaagc
     3781 tgaatttgac aaaaaatgat atagttattg gtatcgcagc tagcggtcgt acaccatatg
     3841 tgatcggtgg attagattac gcaaaatcga tcggtgctgt cacaggaacg atttcttgca
     3901 ataaacaagc cgaaatcagt aaacacgcgg atttcccaat cgaagtcgat gcaggtccag
     3961 aatttttgac aggctctact cgattgaagt caggaacagc acaaaaactt atcttgaata
     4021 tgatttctac tatcagtatg attggtatag gtaaagtatt caataacttg atggtcgatg
     4081 tcaaaccaac aaatgaaaaa ctaatcgaac gctcaaaacg aatcatcatg caagcgacag
     4141 aagctgatta cgaaacagct gaaaagtact ttgaagaagc agatcaaaat gttaagctgg
     4201 ctatcgtcat gattttgaca aatagtgata aagaggaagc tcaagcaaaa ttagctgaag
     4261 caaacggatt tgtaaaaggg accttatgag ggggaaaaga ccatggcaaa aaacgaactg
     4321 actttggaag aactggcaaa acaaatttat aaaggggcag gcggcatggg caatgtagaa
     4381 agcattgtcc actgcatgac tcgtgtgcgt atgaagatca tcaatgatga tcttgtaaat
     4441 gtctctgaat taaaagcgat tccaggagtg cttggtgtag tagaagatga acagcttcaa
     4501 gtcatcatcg gacctgggaa agtgaacaaa gtagcacagc aaatggttga tttggcaggc
     4561 gtgaaattag gagaaaaact ttcttctgta agtacagcta cagcaaattc atcagcatct
     4621 ggaaaagacc gtgtaaacga acgggcacaa gaaatgaaag cggcacaaaa atcgaaacaa
     4681 aaaccatcta aaatcaaatc ggtattaaaa gacatttcta atatctttgt gccaatgatt
     4741 cctgcatttg tcggtactgg gatcgtggca gggattgccg cagttttagc aaacttagtc
     4801 acagccggaa cacttgatgc agcaacatgg caacaatata tcgatgtgat gaacatcttg
     4861 aaaaacggga tgtttagcta cctagttatc tatacaggta tcaatgctgc acaagttttt
     4921 ggtgcaacac caactcttgg tggagttatc ggggccgttg tgatgctgac agggatgaat
     4981 ccggaagcac cactgaaaaa cttatttact ggtgaagctc ttgcagctgg acaaggtggg
     5041 atcctaggtg tcatcttcgc tgtttggcta ttgtcaatcg ttgagaaaaa actgcataaa
     5101 gtagtacctg atgcggtaga tatcatcgtt acaccaactc tttcattgat tgccatcggt
     5161 ctgattgaaa ttttcttcat catgccttta gcaggtttca tttctgatgg catggtcggt
     5221 gggatcaact gggtacttgg cgttggtgga gcatttgccg gcttcgttct aggcacatta
     5281 ttcttgccga tggttatgtt cggactgcat caaattttga ctccaatcca tgtgcaaatg
     5341 attgatgaaa ctggtcgtac gctattgctt ccaattcttg cgatggctgg tgcaggacaa
     5401 gtgggtgcag cattggcatt atggattcgc tgcaaaaaag ataaagaatt aacagaaatg
     5461 atcaaaggag ctttaccagt cgggatccta ggaatcggcg aaccattgat ttacggagta
     5521 acattacctc taggacgccc atttattact gcttgtatcg gtggaggtat cggcggggca
     5581 gtgatcggaa tgatcggaaa tgtcggggct atcgctattg gaccttcagg agccgctttg
     5641 atcccattga tctctgacgg taaatggtta ggctacatcc ttggattatt ggcagcttat
     5701 gctggtggct tcgttgctac attcttcttc ggtattccta aagaccgttt agaaaaagaa
     5761 gaattagcga ctgaaacagc cacagtaaat gaagtgcctg aaaaagtagt acctcaaact
     5821 tcagctgaaa gtgtgactat cctctcatca gttgctgacg gaacagttgt ttctttagat
     5881 aaagcaagtg atccagtttt tgcacaaaag atgatgggtg aaggctactt tgtagaacca
     5941 gaaaacggac atatctattc tccagttact ggggtagtca gctctgtctt cccaacgaaa
     6001 cacgctattg ggatcacaac gccaaatggt ttagaaatct tgcttcatat gggaatcaac
     6061 acagttgact taggcggtac gccatttgat gtgaaagtaa cagaagggca gcaagtgaca
     6121 cctgatacgc ttgtagcaga agctgactta gaagcgatca aagcagcagg caaagaaaca
     6181 tccatgatcg tcttggtaac caatatggat cgtgtaaaaa actttgtctt ggaaaaaaca
     6241 ggtaaagcaa aagcaaaagc acctgtgatg gatgtcaaag cttaatatga tagaaacaat
     6301 agaaacgaaa gaagaaactg aaatctattt ttcagcttct tcttttttat tatttattgt
     6361 tttctgctaa aatctgataa attcgtgtca catcagatgg tgttccccgt aattcataat
     6421 ctgccaagaa aggaaagcca aatgtttgtg catattgttt ggctgttaga caatactgat
     6481 tattgaagtt tttatttccg ctaccgacta caccaagaca aagtttatga ttgtcttcat
     6541 gttccagata ttcacgcatc gtttcggtca agatttctgt atcaccgtta tctaaaccat
     6601 ttccgccgtc taaataagta ggaacgaaag taaaaaatgg ttctgtttct ttcgtaaaat
     6661 cactattttc atggatttct tttaaagtaa cttcaggatt gacaggatct tctgcatgca
     6721 ttttttcagc atattctgct agatgtttgg caaaggcacg cgtatttcct gaaatcgata
     6781 tatataaaat gttcatggat catcctccaa ttctttcctt ttagtatagc atctttttta
     6841 agttttttgg tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata atgagaaaaa
     6901 agccggtaga attatatttc tattaaagtt tttattaatt aagtaaaaat aaaaaaggtt
     6961 tgttatactt tttgataaaa gcggttttat tttggaggat gcttacatgg atacagagca
     7021 acagaaaaaa ggaattggtt taattccact tgctgcctta gtaattggtt cagcgattgg
     7081 aggtggcgta tttggtatta tgacggacat ctccggatcg gctggcggcc cagctatttt
     7141 cagttgggta ctcgtaggaa cagcaatgtt gatgctctcc ttgtcgatca ataatatcaa
     7201 taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag gttttggccg
     7261 tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag gcaacgtagc
     7321 ttttgcaaca ttgttgatga gcgcattagg atatttcttc ccagtatttg aaggtgggca
     7381 aaatctggca tcgatcgtga tgagtacagt ctttttatgg ggatatgcat ggctagtgaa
     7441 tcggggagtc gaaaatgcta gtttcgtcaa tgctatcgtc acattctgta aattagtgcc
     7501 attattttta tttattgtag tagctatcag cacgttttcg atccaacaat tcattgataa
     7561 cttttctagc gacattatat tgaatgcgaa tggacagccg atcccgtttt ccagtcaagt
     7621 attgagttgc atcatggtga tgctttgggt atttgttggt attgaaggag cctctgtcgt
     7681 aggttcacga gcaaaaaaac aatcagatgt tgggaaagca acgattctag ggatattcgg
     7741 cttgattatt atttacgtct tggtttccat gcttccatat gggacaatga cgatgaatca
     7801 gctgcaaacg atcaaggcgc agcctgctat gggctatgtg tttgaaatga tggttggtaa
     7861 atggggagca gtcgtgatca acggcggttt gatcatttct ttagttggtg tttggctctc
     7921 atggacgatc ttaccgattg aaacaatggg aaacatggct gaagacggac ttttgcctaa
     7981 agcatgggga aaactcaaca aaaatggggc accaacttat gctatcgtat tgacgacgtt
     8041 atgtacgaat gtctttttac tttcattatt attcactgac agtgcttata actttgctta
     8101 cacacttggt acagcagcta ttttcttcac gtggcttttc ttaggattgt accaaatgaa
     8161 actttcttat cagcggaaag aatgggtcca atttttagta gggcttttag caagtgcctt
     8221 tcaagtatgg gctatgctag tcgtagcctt taatgaagta atgcttgttc ttgttctttt
     8281 cttgccaggc atttattttt acctcaaagc acgtaaggag caaaacgagc ctgtacaaca
     8341 tatccactta gataagattt tacttatagg tgtgacgatg gtcggagtga ttgcaatggt
     8401 tttatttgcc actgggatgc ttcacgtata gaagtataaa agaagtataa agagaggttg
     8461 tgagcctgtc gtttattcaa aaacatacta ggaaaggagc tgtgacacaa atg
//
LOCUS       DAYYKN010000330         4401 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.guided.181, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000330 DAYYKN010000000
VERSION     DAYYKN010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.guided.181"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..903
                     /gene="vanY"
                     /locus_tag="ACHBB7_003049"
     CDS             97..903
                     /gene="vanY"
                     /locus_tag="ACHBB7_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFM9210897.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            921..1748
                     /gene="vanW-B"
                     /locus_tag="ACHBB7_003050"
     CDS             921..1748
                     /gene="vanW-B"
                     /locus_tag="ACHBB7_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFM9210898.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1745..2716
                     /locus_tag="ACHBB7_003051"
     CDS             1745..2716
                     /locus_tag="ACHBB7_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM9210899.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2709..3737
                     /gene="vanB"
                     /locus_tag="ACHBB7_003052"
     CDS             2709..3737
                     /gene="vanB"
                     /locus_tag="ACHBB7_003052"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM9210900.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3743..4351
                     /locus_tag="ACHBB7_003053"
     CDS             3743..4351
                     /locus_tag="ACHBB7_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM9210901.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         1246 a          921 c         1184 g         1050 t
ORIGIN      
        1 gaatgatttt ctatcagtcc tagaaattat agagccataa taacaatcgt accagccaca
       61 aatcgtagtt ttattgcaaa ggaggcattc gatcaaatgg aaaaaagcaa ctatcattcc
      121 aatgcggatc atcacaaacg gcatatgaaa caatccgtgg aaaagcgggc ttttctatgt
      181 gcgttcatta tttcgttcac agtctgcacg ctgtttttgg ggcggagact ggcttccgta
      241 ttggaggcaa cacagatacc gcccatccct gcaactcata caggcagcag cactgacgta
      301 gtggagaatt tggaggaaaa cgctcttgcc accgccaaag aacagggaga tgaacaggaa
      361 tggagcctga ttttagtgaa caggcagaac cccatccccg cacagtacga tgtggagctt
      421 gagcaactat caaatggtga gcggatagat attcggattt ctccctatct tcaagatttg
      481 tttgatgccg caagaactga tggagtttac ccgattgtcg catccggata ccgaacaaca
      541 gaaaaacagc aagaaattat ggatgaaaaa attgccgaat ataaggcgaa aggctacacc
      601 tctgcacagg ctaaagcgga agcagaaact tgggtggccg tgccgggaac gagcgagcat
      661 cagcttggtc ttgctgtgga tatcaatgcg gacggaattc attcaacagg caacgaggtt
      721 tatagatggc tggatgaaaa cagctatcgt tttggtttta ttcgtcgcta cccaccagac
      781 aagacagaga taaccggtgt gagcaacgag ccgtggcatt accgatatgt tggcatcgaa
      841 gctgccacag agatgtacaa ccaaggggtt tgccttgagg aatatttaaa accagaaaaa
      901 tgagaaaagg atgttatgtt atggacagaa aaagattgac acagcgtttc ccgttcctgc
      961 ttccaatgag acgggcgcag agaaaaatgt gcttttatgc gggaatgaga tttgacggct
     1021 gtcgctatgc gcagacgata ggggaaaaat cgctttccca tttgctcttt gaaacggatt
     1081 gcgcattata caaccacaat accggatttg acatgatata ccaggaaaac aaggtgttca
     1141 acttaaagct ggcagcaaag accttaaacg gcttattgat aagaccgggg gaaccctttt
     1201 ctttctggtg gctggtacgc catgcggaca aagatacccc ctataaagac ggccttacgg
     1261 tgaccaatgg taaactcacc accatgtcgg gcggcggtat gtgccagatg agcaatttac
     1321 tattttggat gttcctgcat acgccattga caattatcca gcgcagaggt cacgaagtaa
     1381 aggagtttcc agagccaaac agtgacgaga ttaaaggggt ggatgcaacc atttcagagg
     1441 gctggattga tttaaaagtg cgaaacgata ccgactgcac ctaccaaata tgggtgacct
     1501 tagatgatga gaaaatcatc ggtcaggtgt ccgccgacaa agagccccaa gcattataca
     1561 aaattacaaa tggcagtatc cagtatgtcc gtgaaagtgg cgggatttat gaatatgccc
     1621 aggttaaacg gatgcaagtt gccttaggta ccggggaaat aatagattgc aagctgcttt
     1681 atacaaacaa atgcaaaatc tgttatcccc tcccggaaag tgtggatatt caggaggaga
     1741 accaatgaga aaaagtgtgg gcattactgt ttttggatgc gaacaggatg aggcaaatgc
     1801 tttccgcgct ttatcgccgg attttcatat tatccctacg ctgattagcg atgcgatatc
     1861 ggcagacaac gcaaaattgg ccgctggcaa tcaatgcgtt agcgtaggcc ataagtccga
     1921 ggtttccgag gcgacaattc ttgcgctgag aaaggtcggg gtaaaataca tttctacccg
     1981 cagcatcggc tgcgatcaca ttgatacgac tgccgccgag agaatgggaa tctcggttgg
     2041 cacggttgcg tattcgccgg acagcgttgc ggattatgct ttgatgctga tgctgatggc
     2101 catacggggt gcaaaaccca ccatgcacgc cgtggcgcaa caagatttca gattggatcg
     2161 tatccggggg aaagaactgg gggatatgac tgtgggagtt attggaaccg gccatatcgg
     2221 gcaagcggtc gtcaaaaggc tgcggggatt tggatgccat gtgctggcct atgataacag
     2281 ccgaaaaatg gatgcagatt atgtccagct tgatgagctt ctaaaaaaca gcgatattgt
     2341 tacgctccat gtgccgcttt gtgcggatac ccgccatctg atcggtcaga agcaaattgg
     2401 agagatgaag caaggcgcat ttttaatcaa caccgggcgc ggagcgcttg tcgataccgg
     2461 ggcgctggtg gaggcgttgg aaagcggaaa gctgggtggt gcggcactgg atgtgttgga
     2521 gggcgaggat cagtttgttt ataccgattg ctcgcagaaa gtgcttgacc atcccttttt
     2581 gtcgcagctt ttaaggatgc cgaatgtgat catcacaccc catacggcgt actacactga
     2641 gcgtgtgctg caggatacta cagaaaaaac aatcaggaat tgtctcaatt ttgaaaggag
     2701 tttacagcat gaatagaata aaagtcgcaa tcatcttcgg cggttgctcg gaggaacatg
     2761 atgtgtcggt aaaatccgca atagaaattg ctgcgaacat tgatacggaa aaattcgatc
     2821 cgcactacat cggaattaca aaaaacggtg tatggaagct atgcaagaag ccatgtacgg
     2881 aatgggaagc cgacagtctc cccgccatac tctccccgga taggaaaacg catgggctgc
     2941 ttgtcatgaa agaaagcgaa tacgaaacac ggcgtattga tgtggctttc ccggttttgc
     3001 atggcaaatg cggggaggat ggtgcgatac aggggctgtt tgtattgtct ggtatcccct
     3061 atgtgggctg tgatattcaa agctccgcag cttgcatgga caaatcactg gcctacattc
     3121 ttacaaaaaa tgcgggcatc gccgttcccg aatttcaaat gattgataaa ggtgacaagc
     3181 cggaggcggg tgcgcttacc taccctgtct ttgtgaagcc ggcacggtca ggttcgtcct
     3241 ttggcgtaac caaagtaaac ggtacggaag aacttaacgc tgcgatagaa gcggcaggac
     3301 aatatgatgg aaaaatctta attgagcaag cgatttcggg ctgtgaggtc gggtgtgcgg
     3361 tcatggggaa cgaggatgat ttgattgtcg gcgaagtgga tcaaatccgg ctgagccacg
     3421 gtaccttccg catccatcag gaaaacgagc cggaaaaagg ctcagaaaat gcgatgatta
     3481 cagttcccgc agacattccg gtcgaggaac gaaatcgggt gcaggaaacg gcaaagaaag
     3541 tatatcgggt gcttggatgc agagggcttg cccgtgttga tctttttttg caggaggatg
     3601 gcggcatcgt tctaaatgag gtcaataccc tgcccggttt tacatcgtac agccgctacc
     3661 cacgtatgat ggccgccgca ggaatcacgc ttcctgcact gattgacagc ctgattacat
     3721 tggcgttaaa gaggtgataa gtatggaaaa tggttttttg tttttagatg aaatgttgca
     3781 tggcgtccgt tgggatgcca agtacgccac atgggataac ttcacaggaa aaccggtgga
     3841 tgggtatgag gtgaatcgca tcatcggcac aaaggccgtg gcgtttgctc tgcgcgaggc
     3901 acaaatccat gcggctgcgc ttggctatgg cttgctttta tgggatggat atcggccaag
     3961 aactgcggtg gactgcttcc tgcgttgggc agcgcaaccg gaggacaatc tcacaaaaga
     4021 aaaattttac cccaatatag agcgagccga gttgattaca aagggttatg tggcctcaca
     4081 atccagccat agccgtggaa gcgcaattga tcttacgctc taccacctgg atacagggga
     4141 acttgtttca atgggaagta acttcgattt tatggacgaa cggtcgcatc atacagcaaa
     4201 agggataggg gatgcagagg cacaaaatcg aagatgcttg cgtaaaatca tggaaagcag
     4261 cggatttcag tcttatcgct ttgaatggtg gcactataag ttgattgatg agccataccc
     4321 cgatacctat tttaattttg ccgtttcata atgaaagtat tggttttgta tcagattttg
     4381 gatttgataa aatctatgct g
//
LOCUS       DAYYKN010000331         3180 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.guided.207, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000331 DAYYKN010000000
VERSION     DAYYKN010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.guided.207"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..499
                     /locus_tag="ACHBB7_003054"
     CDS             35..499
                     /locus_tag="ACHBB7_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9210902.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            483..1112
                     /locus_tag="ACHBB7_003055"
     CDS             483..1112
                     /locus_tag="ACHBB7_003055"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9210903.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1466..2944
                     /gene="msr(C)"
                     /locus_tag="ACHBB7_003056"
     CDS             1466..2944
                     /gene="msr(C)"
                     /locus_tag="ACHBB7_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM9210904.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2991..>3180
                     /locus_tag="ACHBB7_003057"
     CDS             2991..>3180
                     /locus_tag="ACHBB7_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM9210905.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAK"
BASE COUNT         1106 a          520 c          680 g          874 t
ORIGIN      
        1 cgttgacgag tcaattatta gaaaggagga aattatgtct ttagaagaga ttagtcacct
       61 tttatatcaa atcaaattag ccgatcaaaa aatcgtccag atctttgaag aaaatatcgg
      121 tataagtctt actagatacg agatcatgat gagactgaaa gaagaaaatc cattgctaca
      181 aagcagtttg caagaaaagt tgcagattga tcaaggagca gttactcgtc atctgaaggt
      241 attggaagca aatggctatg tttcgcgtca gagaaatcct cagaacaacc gggaagtgta
      301 tgtagcgcta acagaaaaag cccagaagaa aatcgagcat tgtgaaatgg accataaaga
      361 tacaggttcc gtgctatccc cggctttcag cgatgtagaa atgaaacaat ttctaacact
      421 tttagataaa ttgaataccc aactttgcaa tataacaaca gaaacgaaag aggttaataa
      481 taatggaaca aaaaaatgat ttttcaacaa ttatgactgg tagaagatcc gttcgcgtat
      541 atgatgaaac atacaaaatc ccacacgaag aaatgctgga aatgatcaaa gaagcgacga
      601 ctgcaccttc ttctgtcaat atgcaaccat ggcgctttgt ggtcgctgaa agcgaggaag
      661 caaaagaaat cttgcgtcct ttgatccgtt tcaatacgag acaaaatgat acttcttctg
      721 caatggtcat gatttttggc gatatgcaat gttacgaata tggcgaagaa atctataacc
      781 aagcttacga atcagggaag atgccaaaag aagtcagaga tcaacaatta gctgcgatca
      841 ttccttatta caagagtttt tcaagagaag aaatgaacga tgtagtcaaa gtcgattcta
      901 gcttagcggc tatgcaattc atgttagttg cgcgtgatca tggctatgaa acaaatccaa
      961 ttggtggagt cgaagcagat cagttggcag aggcatttgg tttggataaa gaccgttatg
     1021 ttccagttat tattttatca ataggaaaag cggtagaaga aggttacgag tctgttcgtt
     1081 tagcaccaga aaagatcact acgttcgaat aaaaggtttt cagttactat tcagtagcta
     1141 ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag gaggtcggaa aaatgactgc
     1201 atcgatgaaa ttacgtttcg aacttttgaa taacaactaa ataaattcaa aagacatgta
     1261 taaacaacaa tttcatggat ggagtatgtc cattttttcg aacgacagta gaataggtga
     1321 aagctatgac agatttggtg atttagtcat agttatttcg ttcagagtag gaaaagatgt
     1381 gatgcagttt ttttgcaaac agatttttct gctctttttt agtatcgata attgttgttt
     1441 tacatgaaat aggagtgtaa aacatatgga aaatttagca gtaaatataa caaatctaca
     1501 agtcagcttt ggcaaccagc tagaactatc tattgattct cttcgtgtct atcagcaaga
     1561 tcgtataggg atcataggag aaaatggtgt cggtaaatcg actttgctca aactaatagc
     1621 cggtgaactt tttcccgatc atgggaaaat ccaaacagag atcaccttca actacctgcc
     1681 tcaattaacc tatctcgctg aagcaaagga cctaaatttg gaattagcca gtcatttcca
     1741 gttaagactg gaagaaactt cagagcggaa atggagcgga ggggaagaac gaaagatcga
     1801 gttgatacgt cttctttctt cttatgaaca agggatgctt ctagacgagc caacaaccca
     1861 tctagataga aaaagtattg atcgactgat tgaagagctt cgttattatt atggtacgct
     1921 ggtttttgtt agtcatgatc gctattttct agatgagcta gcatcgaaaa tctgggaagt
     1981 aaaagacgga gaaatccgag agttttcggg gaattatagt gcctatctca ctcaaaagga
     2041 attggagaaa aagactcagc tacgagaagc agagtcgatc atgaaagaga aaaaacgatt
     2101 ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag tgtccagtaa
     2161 aaagaaaaag caacaaatca gaccggatcg gttgtcttcc tctaaacaaa aagacagtgt
     2221 acaaaaagcc atccaaaaga atgcgaaaac attagagaga agactccaaa aaataggaga
     2281 aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat ctcttgaact
     2341 ccacagccgc tatcccatca tgggacaaaa tgtccaattg gaaagaagtg ggagaacatt
     2401 actggtaaat ggtgattttc agttttcttt aggtaaaaaa atcgcgattg tcggtgaaaa
     2461 tggttcaggt aagacaacct tattagaaca tatccgcaaa caaggagaag gaatccttct
     2521 ctctccgaaa gtaagctttc aagtatatca gcaaaagggt tatcaaatga catctgaaga
     2581 atccatcatt cgttttgtca tgagacaaac agagttttca gaatcgcttg tccgtagttt
     2641 gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta cattaagtgg
     2701 gggagaagcg acccgtctga cgattgcttt gctttttact aagccaagta atgtgttgct
     2761 gttagatgaa ccgactaatt ttattgatat ggcaacgatc gaagctttag agaagctaat
     2821 gcaaatatat ccgggaacga ttttgtttac ttctcatgat tcttactttg tcgagcgtac
     2881 ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa aaagtactta cgagaaattt
     2941 ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga gtggtacaga ttgcctacaa
     3001 ataaaaaaga aggaataatt tttacaacaa tgatgtgttt tttgatggtc ttggggatga
     3061 gcgcgtataa tttatggctg catcaagatc ttcatttatc aagtttgttg attggtctag
     3121 tgcctggatt cgtggtggct ttcattttgg atgtcttcat catcggtgtg ctggcgaaaa
//
LOCUS       DAYYKN010000332         2929 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.guided.215, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000332 DAYYKN010000000
VERSION     DAYYKN010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.guided.215"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..362
                     /locus_tag="ACHBB7_003058"
     CDS             <1..362
                     /locus_tag="ACHBB7_003058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210906.1"
                     /translation="TAVFNSNQSVLLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPV
                     LEQGIKDKSLEIENSHEIAEVIMVLSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGI
                     DTLLDEETISAFIQFCK"
     gene            469..1707
                     /gene="mef(H)"
                     /locus_tag="ACHBB7_003059"
                     /gene_synonym="mefH"
     CDS             469..1707
                     /gene="mef(H)"
                     /locus_tag="ACHBB7_003059"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM9210907.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            1934..2353
                     /locus_tag="ACHBB7_003060"
     CDS             1934..2353
                     /locus_tag="ACHBB7_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM9210908.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            2358..2597
                     /locus_tag="ACHBB7_003061"
     CDS             2358..2597
                     /locus_tag="ACHBB7_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9210909.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(2822..>2929)
                     /locus_tag="ACHBB7_003062"
     CDS             complement(2822..>2929)
                     /locus_tag="ACHBB7_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM9210910.1"
                     /translation="AVVQEYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT          939 a          411 c          533 g         1046 t
ORIGIN      
        1 agacagcggt attcaattct aaccaaagcg ttttattgac agtttctcct caactattaa
       61 acaatcctcg ttttcttgct atgcaaatag aacaaatgta tgaaattgta gtacctgaat
      121 ttgttgaacc cgttttagaa caaggtatca aggataagtc tttggagatt gaaaattcac
      181 atgaaattgc agaagttatc atggtattaa gtaatgtatg gctcaaccca ttagttgaaa
      241 tgacggataa agagggtatg gaaaaacgtt gcaatacatt caatgccctt ttaaatggaa
      301 taggcataga cacattatta gacgaagaaa caatttctgc atttatacag ttttgtaaat
      361 aactgtattt gttatcccta tacgatagac tgctctataa aagagcagtt tatttaaacc
      421 attatataaa ccgacggtct gtttgattaa ataaaaagga gagattttat gcaagcagaa
      481 aaaaaacttt ttaatcgtga ttttatactt gttgttatag gacagataat ttctttattt
      541 ggaaacgcta tcctacgatt tgcattaccg tactatttgt taaaagaaac tggttcatca
      601 gctttgtttg gtattgctac agcctgctct tttttaccta tggttatttt gtcgctggta
      661 ggtggtatta ttgctgacag gacaaataaa agaaacatta tggtcatact agattttgta
      721 acagcaatca taattttagt attttatttg tcattgggaa aaattcctat catacctttg
      781 tttattgttg ctttgatgtt actttatggt atttctggtg catatcaacc agcggtacaa
      841 gcaagtattc ctctattggt atcatcagat aaacttaccg tcggtaatgc tataataaat
      901 caagtaaata ctttagcaaa ctttatcgga ccagctataa gcggagtagt ttttcaattt
      961 tatggtatca atcccatttt aattgttagt attatatgtt ttactttttc agccattatg
     1021 gaaattttta tccatattcc acaccaaaaa agacctcgtg aagtaggtgt aattgcagtt
     1081 gctaaaaacg atttaaaaga tagttatacc tttttgaaaa aggagaaacc catttttata
     1141 tccgtaatct ttttggtttg cattttcaat ttggttttaa gtgctgtttt aattgttggt
     1201 ctgcctatct taatttctga agttttaaaa atgccagata cactatatgg tttttcacaa
     1261 tcagcattat cattaggtgg tttatgtggt ggtattttaa cagctcttgt tgcttcaaag
     1321 ttaaaactaa aaaaccttta taccttgtta cttatttgtt ctgtttctgt tttacttatg
     1381 ggaatttcgc tatttttaga attgcctgca atagtttcct actgggtagt tacagtgatg
     1441 tgctttgtgg ctatgggtgt atctactatg tttgttgttc aaatttacac tcttgttcag
     1501 acacaaacac caccgcaatt agtaggaaag ataatggcta cgctggtagc agttgctatg
     1561 tgcggtcaac ctattggaca aatggtttac ggtattttat tcgatgtatt tatacaagaa
     1621 acatgggttg ttatgtttgt agcttctatt gcttcaatca tgatttcaat atattcaaaa
     1681 agaatttttc aaaagttaga gatataatac aaggggttaa atagtatgtt aggctaaaaa
     1741 caatatataa aagaaaattt gaaatctgct aaaaggatag tgttataaaa gaaagagtaa
     1801 tattgcagta tttaaggctc gcacaaatat atgttttgtg tgggcttttt tcttaccatt
     1861 tttctgtatt tctaaagggt ataaacggta ttccgtttat atatttcagc attagcagaa
     1921 agggggtgag attatgagga catcttcttt cgagcatatc gtcagattac agttcaattc
     1981 tttaatgctg attgtcatta agaacaaagt gaaaagtcga aacagacagc ttgcaagaca
     2041 ttctaaacgt gaagtattgt tttgtgaaat agccgaaacg aaacaagttg aacgtggaac
     2101 gattgatacc tgtttttatg attgtgtatc atttaaagtc ttgcacttta caatttatgt
     2161 atctgatgaa acacttggta cagttttaca tagactgtca gaaaaacagc gtagtgcgat
     2221 tttattacgt tattttcaag gaatgaacga cagagaaatt tcagaactgt accatgtatc
     2281 acgttctgct atttgcagta gaagaactag aggactaagg aagttaaaaa tgcttttgag
     2341 cgaaaggaag taaagaaatg agtaaagatt atggctatcc ctcttttgaa ttgatttgca
     2401 aagcgtcaag cggagatgaa atggcagtta aagagatttt gaagttttat gacggttata
     2461 tttctaaatt atgcttgcgt cctttttacc actctgaaag tggtaaaatc attatgcagg
     2521 ttgatgaaga actaaaagga gaaattcata cagacatgat gaaagcaata ttgaaatttg
     2581 aaatcggagt gaagtaatca actgtataaa aaaatcggga gatacacagt ttcgtgctat
     2641 ctcccttagt agttttacgg tcatatcaag gctcattcaa tcgtggtaaa ttcgtggtaa
     2701 gatgagtgtt tttctatact tcaaaatgct tgaatgtata gtgtttatgc ggataattga
     2761 aaattagata taaaatttca aataaattac tgatataaat ttacaataag aactcttgtt
     2821 tctacttttt tttatgagaa aatttagttt tcaatttatt gaaccatttt cggcttggat
     2881 ctcgacgttt tttgatttga tgacccgtca cgtattcttg aacaactgc
//
LOCUS       DAYYKN010000333         2772 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.guided.220, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000333 DAYYKN010000000
VERSION     DAYYKN010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.guided.220"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..194
                     /locus_tag="ACHBB7_003063"
     CDS             111..194
                     /locus_tag="ACHBB7_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM9210911.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="ACHBB7_003064"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="ACHBB7_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM9210912.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1319..2182)
                     /locus_tag="ACHBB7_003065"
     CDS             complement(1319..2182)
                     /locus_tag="ACHBB7_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM9210913.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2184..2456)
                     /locus_tag="ACHBB7_003066"
     CDS             complement(2184..2456)
                     /locus_tag="ACHBB7_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9210914.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2473..2682)
                     /locus_tag="ACHBB7_003067"
     CDS             complement(2473..2682)
                     /locus_tag="ACHBB7_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM9210915.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          488 c          443 g          990 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta
     1141 ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat
     1201 ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt
     1261 tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt
     1321 aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc aagctttcca
     1381 ttttttgttg aattgcttta aattcagggg tttcttggtg ttgattttga accatttttt
     1441 gctctattct ctccaaagtc ggttgaattt ctttccctga tactttacga tttaattctc
     1501 tttctaaggt ctctttcgga ctaatggaag gcgtttctaa gcttgaatag agtttcactc
     1561 cttctctatt gtaaagcctt atatcgctaa ataagcccgt tttatgaagg gtttctaaat
     1621 tggtcggtaa gtttttgaca acaatatcat gcgcttgttt tggtgttgcc ctagctgtca
     1681 ttggatcatc tgcatacatg gtttcatatc gttcaatcgt tcctaagtat gattcaattt
     1741 tcggtactgc catgacgtat atttttgttt cataaccttt agattgaagc attgtggctg
     1801 tttgaatggg aacgtctgtt gttcgtcctg ttccttcaat cactaaatta tagccttgat
     1861 cgcttaaacg gctaataagt gcttctgtca tgcgattcga ataaggggta acgtgtttta
     1921 ctacgtcttt ttcatagagt ttcactaatt cgtcaaaatt tgggtgctgt tgtttaaagg
     1981 tatcattatc aatgacaata acattccctt gtgtttcttc aaaaattgct gatcgcaaac
     2041 tggttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgcta
     2101 gtctattttt agttagtcgt tcaacattct tttctaaacg atcttcaaat tgcttttcgg
     2161 tgaagtcagt aatattcgcc atgttaagcc actttctctt tattcaaaat ttgttttgaa
     2221 tattgattca ttgaccgcca atactcatga atagcttgta attcttcaaa tggttgttga
     2281 aataaatcaa cttcttgtgc cacttctaat tgatttaaaa gattcacttc tagtaaacga
     2341 gtatttttag tatctagttc atgattcaaa acataattta atacccgatt ataaacactt
     2401 gccgataact cattaatgat ttcaatttca aatgtttttt catacgtaac tgccatttca
     2461 ttttcactcc ttttaaagtt tgtcaggtaa atatttccgt atatattctt ctaaggcttg
     2521 atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta tgatatggtg
     2581 caagtcggca cgaacacgaa ccgtcttatc ccccattata tctttttttg cgctgattgg
     2641 cgtatcattt cgttttgctt tctgtgcgcc taaatttccc acgatcgctc acttctttct
     2701 atttcttctt actatttatt ttatcatcac ctatcacaaa tcacaagtga tttgtgatta
     2761 atcacttgtg at
//
LOCUS       DAYYKN010000334         2563 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.guided.230, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000334 DAYYKN010000000
VERSION     DAYYKN010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.guided.230"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..221
                     /locus_tag="ACHBB7_003068"
     CDS             <1..221
                     /locus_tag="ACHBB7_003068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210916.1"
                     /translation="VRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            434..1093
                     /gene="vanR-B"
                     /locus_tag="ACHBB7_003069"
     CDS             434..1093
                     /gene="vanR-B"
                     /locus_tag="ACHBB7_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM9210917.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            1096..2439
                     /gene="vanS-B"
                     /locus_tag="ACHBB7_003070"
     CDS             1096..2439
                     /gene="vanS-B"
                     /locus_tag="ACHBB7_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM9210918.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT          752 a          526 c          638 g          647 t
ORIGIN      
        1 gagtgcggca agagcgaccg gagggagtac acacaggccc tgcctgtgtg aaaagccata
       61 ccgcaaggtg cattcgcaaa aaagaggccg cgcattttgc tgaggcttgg ggtgtcccca
      121 acaagcaaaa cgagaacgcc gcaggcgggc aagtggtgga tcagaatatc cgtaaaggat
      181 attctgatcc attgcttgcc agtattatcg ggatgttttg acaaccgctg taatcgaaac
      241 atcccaaaca ttaagaaggt gttaattatt attatcttga tttaattcgt ttaaaccaat
      301 aatatagata catacaccat gaaaagaagc gagtatccca atgacggatg attaaaatcc
      361 gaaaagaaat cgctttgaaa ctacagggaa actacagact gttatgttat cttcttaaaa
      421 tggagggatt tttatgtcga tacgaattct acttgtcgag gatgatgatc atatctgtaa
      481 tacagtaagg gcgtttctgg ctgaggcagg atatcaggtg gatgcctgca cagatggaaa
      541 tgaggcatac accaagtttt acgaaaacac ttatcaactg gttattcttg atattatgct
      601 gcccggtatg aacgggcatg aacttttgcg tgaatttcgt gcgaaaaatg atactcccat
      661 tctgatgatg acagccctgt cggatgacga aaaccaaatc cgggcgtttg atgcagaggc
      721 agacgactat gtaacaaagc cattcaagat gcagatttta ctaaagcggg tggaagccct
      781 gctgcggcgc agcggtgcgc tggcaaagga aatccgtgtc ggcaggctga cacttctgcc
      841 ggaggatttc acggtacttt gtgacggtac agagctgccc ctgacacgaa aagaatttga
      901 aatccttttg ctgctggtgc agaacaaagg cagaacctta acgcatgaaa tcattttgtc
      961 acgtatatgg ggatatgact ttgagggtga tggcagcaca gtccacactc atatcaaaaa
     1021 tttgcgggcc aagctgccgg aaaatatcat caaaaccatt cgcggtgtag gataccgatt
     1081 ggaggaatca taataatgga aagaaaaggg attttcatta aggttttttc ctatacgatc
     1141 attgccctgc tactgcttgt cggtgtaaca gcaacactgt ttgcacagca atttgtgtct
     1201 tatttcagag tgatggaatt acagcaaaca gtaaaatcct atcagccatt ggtggaactg
     1261 attcagaata gcgataggct tgatattcaa gaggtggcag ggctgtttca ctacaataac
     1321 caatcctttg agttttatat tgaagataaa gagggaagcg tactctatgc cacaccaaat
     1381 gccaatacat caaatagttt tagacccgac tttctttatg tggtacatag agatgataat
     1441 atttcgattg ttgctcaaag caaggcaggt gtgggattgc tttatcaagg gctgacaatt
     1501 cggggaattg ttatgattgc gataatggtt gtattcagcc ttttatgcgc gtatatcttt
     1561 gcgcggcaaa tgacaacgcc gatcaaagcc ttagcggaca gtgcgaataa aatggcaaac
     1621 ctgaaagatg taccgccgcc gctggagcga aaggatgagc ttggcgcact ggctcatgat
     1681 atgcattcca tgtatgtcag gctgaaagaa accatcgcaa ggctggagga tgaaatcgca
     1741 agggaacatg agttggagga aacacagcga tatttctttg cggcagcttc tcatgagcta
     1801 aaaacgccca tcgcggctac aagcgttctg ttggagggaa tgcttgaaaa tatcggtgac
     1861 tacaaagatc attctaagta tctgcgcgaa tgcatcaaaa tgatggatag gcagggcaaa
     1921 atcatttccg aaatactgga gcttgtcagc ctgaatgatg ggagaatcgt acccatagct
     1981 gaaccgttgg acatagggcg cacggttgcc gagttgctgc ccgattttca aaccttggca
     2041 gaggcaaaca accagcggtt cgtcacagat attccagccg ggcaaattgt cctgtccgat
     2101 ccgaggctgc tccaaaaggc actatccaat gtcatattga atgcagttca gaacacgccg
     2161 cagggaggcg aggtacggat atggagtgag cctggtgctg aaaaatgccg cctttttgtt
     2221 ttgaacatgg gcgttcacat tgatgatact gcgcttccaa ggctgttcac cccattctat
     2281 cgcattgatc aggcgcaaag cagaaaaagt gggcgaagcg gtttaggact tgccatcgta
     2341 caaaaaacgc tggatgccat gagcctccag tatgcgctgg aaaacacctc ggatggcgtt
     2401 ttgttctggc tggatttacc gctcacatca acattgtaaa tatttaaaac ttaaatgatt
     2461 ttgaccgaca ggtataaccc tgccggtctt tttttgctgc tacaggaaaa ctacagactg
     2521 gctacaggga aagtacagat gcgcttgcca taataacggc tct
//
LOCUS       DAYYKN010000335         1687 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.guided.255, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000335 DAYYKN010000000
VERSION     DAYYKN010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.guided.255"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..324)
                     /locus_tag="ACHBB7_003071"
     CDS             complement(<1..324)
                     /locus_tag="ACHBB7_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002415466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM9210919.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVE
                     SKKYFV"
     gene            417..766
                     /locus_tag="ACHBB7_003072"
                     /pseudo
     CDS             417..766
                     /locus_tag="ACHBB7_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1038..1328)
                     /locus_tag="ACHBB7_003073"
     CDS             complement(1038..1328)
                     /locus_tag="ACHBB7_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9210920.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          553 a          312 c          239 g          583 t
ORIGIN      
        1 cacaaaatat tttttactct caacatactt gcaaagtaat actacaattc ctaacataat
       61 cgtagaattt aataatttcc atagcataaa gtgtttagaa aagaacatta agaatagttc
      121 tataataatt cttgaactcc atgtcttata atgttctatc gaagcaccca aatagccaag
      181 ttcatctaat ctacttgcat aaactacgtc atccgctctc atagtcggca tatacatatt
      241 aaagagtaaa aagatacccc aaatccctat tagtaacaag atcagttctt tgttattcgt
      301 tatttttcta ttatgtttgt tcatttttat actccttctt atatttccta atagagtata
      361 acatagcttt aagcattact taaagataac ttaaaagtta tcttttaaat acttaattgt
      421 taaatacctt atttgatgca aaagaaatct acgatagtca acaaaaaaat tttcattata
      481 tccagaaaaa tttttcttag ttaaggacct atggattgca gtaatgcaga attcgtcgaa
      541 taaactgcca aagacataca atcatattgc ttttaaaatt gacgaacaag atatagattc
      601 gtttgtctca aaaatccaaa tgttaggttt aactgtcgaa ccaggacgtt ctcgtgtgaa
      661 aggcgaggct tcttcaattt acttttacga ctatgataat cacctcttcg agttacatac
      721 cggtactctc caggaacgtt taaaatccta caattccact acctgagact agtaatgact
      781 agtctctttt tatggcctct gatcccccca ttgagcgaat actaataggg cttcgaattg
      841 atctgttcaa tctttaagca atatccctca aatgagtttg gaaagtttag tgattcatca
      901 caaatactta aatacatgta gttatatata gattgaggtt cttgtgagcg tatgctcgtg
      961 tgggttcaaa tcccatcgtc tgcatccatc tttccaaaac aagcggctag gacatttttg
     1021 tctcagccgc ttgttttcta gtttattctt tcatcttgtc ggaccttttt tcttgggtat
     1081 agacttttct catattctac cacaattttg ggtttcccta acacatgata ggatctcaca
     1141 acaaattgct ttctattatt ataataacct gtaacgacta acttcatccc ttcgtctact
     1201 tcagctagga agttcaaact atgggaagca atcaaacaat tgaccccatt taaactgaaa
     1261 cggatcaacg gtctggcact aacttgcaaa gtacggatct tatctactgt tccatatttt
     1321 ttctccataa ccttcacctc aataccatta tacgaacata cattcccttt gtaaagaaga
     1381 aactctgttt tatctttttg actttttgtc tattaccttt cttaaaatca tattggcact
     1441 tcctgcgaag atgaaggaca tgtactaaaa aaaagaggca gcagtataga tttaagtagt
     1501 tcagttttgg agtacaaaaa gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga
     1561 atttgatact cgttttttat tgttaaatca acgtttgttg ttaatttaca ccacaaaatg
     1621 taagttaaaa tgtatataaa aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg
     1681 tgtgtta
//
LOCUS       DAYYKN010000336         1606 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.guided.262, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000336 DAYYKN010000000
VERSION     DAYYKN010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.guided.262"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..619
                     /gene="dfrG"
                     /locus_tag="ACHBB7_003074"
     CDS             122..619
                     /gene="dfrG"
                     /locus_tag="ACHBB7_003074"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM9210921.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(691..>1606)
                     /locus_tag="ACHBB7_003075"
     CDS             complement(691..>1606)
                     /locus_tag="ACHBB7_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM9210922.1"
                     /translation="DHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRS
                     RFVDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAH
                     HFICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFH
                     EEAISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHS
                     TNSFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKS
                     ADKKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          529 a          237 c          301 g          539 t
ORIGIN      
        1 tatgataata taacttatag aaaaaattgc ttattcaact aacgggcagg ataatgagaa
       61 aaggtttttt gatagaaata agaaattaga tggtaaatta gttaaaaagg ctgtgttata
      121 aatgaaagtt tctttgattg ctgcgatgga taagaataga gtgattggca aagagaatga
      181 cattccttgg aggattccca aggactggga atatgttaaa aatactacaa agggacatcc
      241 gataatatta ggtaggaaga accttgaatc aatcggaaga gccttacctg acagaagaaa
      301 tattattctg acgagagata aggggtttac ctttaatggt tgtgaaattg ttcattcaat
      361 agaagatgtt tttgagttat gtaaaaacga agaagaaatt tttattttcg gaggagaaca
      421 gatttataat ttgtttttcc cttatgttga gaaaatgtac atcacaaaaa tacatcatga
      481 attcgaagga gatacttttt ttccagaagt gaattatgag gaatggaatg aggtatttgc
      541 ccaaaaaggg ataaagaatg ataaaaatcc gtataactac tattttcatg tatatgaaag
      601 aaaaaactta ttgagttaac gggtgcgtta attgaagaag aacagtaaag ataaagcaca
      661 agagcttggg attactccaa gctctttttg tcatttgaaa taaattatat ctttccaatc
      721 aaattgttta tctgtaatcc ctaatctttg agcaggtgtc aaaacctttt tatccgctga
      781 tttaaaaggg cgacaaaagt tgtagtaagt tcttagaatt gtaatcgcaa attgagcata
      841 cttcggattg aagttagcgt aaatatagct cttcttatca ccacgagctg ttgttaatgg
      901 tctttcaaga gatgatatat aacgtcttat ctgttgaata aagctattgg tcgaatggtc
      961 attaacattt aatagcatct ttgcgatttc gtttggttcc agagcagata aatccgttct
     1021 acaatcaact gaataaaatc ctttatcctt agtggctaat ggatgtttga tagggttatc
     1081 ggcatatttg cgatatcttt ctccatcttt actaatagcc tcttcgtgga attgatgact
     1141 tgttagtaat tcagttagat aatgagatgc caaagtccgt agaaactttg ttttgaatcc
     1201 tctgatatct ccccaatcga gcagttctgc ttttgcttgt ccgaattctt ttaagcattg
     1261 ttttctgctt ttcgtcttgt taacttggca aataaaatga tgtgcatcgc tcaaccttaa
     1321 ttctttcgtg aatactctat aaaaggcgtt tctaatagag aagtcatcat cactaatcat
     1381 tcgccattcg tcggcattaa ccatttgttt aattaaccaa tagtgagcag ttgtagtata
     1441 aggagcatca acatttaaac catcgacaaa tctactccta ttagtaattt tccctaattc
     1501 gtgaagataa gcattcctat tttcgctatc gttagcggat ggttcttgtg gataataaga
     1561 ccaatctaac ctatcatttt tacgactaaa agtattgaga tggtct
//
LOCUS       DAYYKN010000337          877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5935
            SAMEA115708052-rid22596053.guided.297, whole genome shotgun
            sequence.
ACCESSION   DAYYKN010000337 DAYYKN010000000
VERSION     DAYYKN010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708052
            Sequence Read Archive: ERR13197777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290204.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,076
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 36 of 85
            Pseudo Genes (incomplete)         :: 48 of 85
            Pseudo Genes (internal stop)      :: 21 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708052-rid22596053.guided.297"
                     /isolate="VRE5935"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            305..853
                     /gene="aac(6')"
                     /locus_tag="ACHBB7_003076"
     CDS             305..853
                     /gene="aac(6')"
                     /locus_tag="ACHBB7_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM9210923.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          314 a          125 c          181 g          257 t
ORIGIN      
        1 taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat
       61 tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc
      121 caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta taatttttta
      181 ttttataagt aagataaata atgaaagaat agagatactg attggtagct aaagattttt
      241 gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag
      301 acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga
      361 tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga
      421 aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt
      481 tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga
      541 aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt
      601 agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac
      661 gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct
      721 tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc
      781 aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc
      841 aaattctcaa taataaaaag aggttgtgac agaagta
//
